LOCUS       DAYYFD010000001        89393 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000001 DAYYFD010000000
VERSION     DAYYFD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.001"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(551..2164)
                     /locus_tag="ACHA25_000001"
     CDS             complement(551..2164)
                     /locus_tag="ACHA25_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8761342.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="ACHA25_000002"
     CDS             complement(2305..3213)
                     /locus_tag="ACHA25_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8761343.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3396..4520
                     /locus_tag="ACHA25_000003"
     CDS             3396..4520
                     /locus_tag="ACHA25_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8761344.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4747..5664
                     /locus_tag="ACHA25_000004"
     CDS             4747..5664
                     /locus_tag="ACHA25_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8761345.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5657..6490
                     /locus_tag="ACHA25_000005"
     CDS             5657..6490
                     /locus_tag="ACHA25_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8761346.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6573..7691
                     /locus_tag="ACHA25_000006"
     CDS             6573..7691
                     /locus_tag="ACHA25_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8761347.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7712..9583
                     /locus_tag="ACHA25_000007"
     CDS             7712..9583
                     /locus_tag="ACHA25_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8761348.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9577..10800
                     /locus_tag="ACHA25_000008"
     CDS             9577..10800
                     /locus_tag="ACHA25_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8761349.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10886..12820)
                     /locus_tag="ACHA25_000009"
     CDS             complement(10886..12820)
                     /locus_tag="ACHA25_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8761350.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13046..13702
                     /locus_tag="ACHA25_000010"
     CDS             13046..13702
                     /locus_tag="ACHA25_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8761351.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13776..14129)
                     /locus_tag="ACHA25_000011"
     CDS             complement(13776..14129)
                     /locus_tag="ACHA25_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8761352.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14321..15250
                     /locus_tag="ACHA25_000012"
     CDS             14321..15250
                     /locus_tag="ACHA25_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8761353.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15261..16097
                     /locus_tag="ACHA25_000013"
     CDS             15261..16097
                     /locus_tag="ACHA25_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8761354.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16345..17121
                     /locus_tag="ACHA25_000014"
     CDS             16345..17121
                     /locus_tag="ACHA25_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8761355.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17297..18040
                     /locus_tag="ACHA25_000015"
     CDS             17297..18040
                     /locus_tag="ACHA25_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8761356.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18033..19613
                     /locus_tag="ACHA25_000016"
     CDS             18033..19613
                     /locus_tag="ACHA25_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8761357.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19864..20343)
                     /locus_tag="ACHA25_000017"
     CDS             complement(19864..20343)
                     /locus_tag="ACHA25_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8761358.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20359..21111)
                     /locus_tag="ACHA25_000018"
     CDS             complement(20359..21111)
                     /locus_tag="ACHA25_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8761359.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21228..21298
                     /locus_tag="ACHA25_000019"
     tRNA            21228..21298
                     /locus_tag="ACHA25_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21260..21262,aa:Gly,seq:ccc)
     gene            21659..22384
                     /locus_tag="ACHA25_000020"
     CDS             21659..22384
                     /locus_tag="ACHA25_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8761360.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22639..22905
                     /locus_tag="ACHA25_000021"
     CDS             22639..22905
                     /locus_tag="ACHA25_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8761361.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22902..23333
                     /gene="ruvX"
                     /locus_tag="ACHA25_000022"
     CDS             22902..23333
                     /gene="ruvX"
                     /locus_tag="ACHA25_000022"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8761362.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23358..23663
                     /locus_tag="ACHA25_000023"
     CDS             23358..23663
                     /locus_tag="ACHA25_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8761363.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23744..24889)
                     /locus_tag="ACHA25_000024"
     CDS             complement(23744..24889)
                     /locus_tag="ACHA25_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8761364.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24950..25597)
                     /locus_tag="ACHA25_000025"
     CDS             complement(24950..25597)
                     /locus_tag="ACHA25_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8761365.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25791..26084
                     /locus_tag="ACHA25_000026"
     CDS             25791..26084
                     /locus_tag="ACHA25_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761366.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26128..26439
                     /locus_tag="ACHA25_000027"
     CDS             26128..26439
                     /locus_tag="ACHA25_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761367.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26436..27060
                     /locus_tag="ACHA25_000028"
                     /pseudo
     CDS             26436..27060
                     /locus_tag="ACHA25_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27201..27359
                     /locus_tag="ACHA25_000029"
     CDS             27201..27359
                     /locus_tag="ACHA25_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761368.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27360..27722
                     /locus_tag="ACHA25_000030"
     CDS             27360..27722
                     /locus_tag="ACHA25_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761369.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27759..28607
                     /locus_tag="ACHA25_000031"
     CDS             27759..28607
                     /locus_tag="ACHA25_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8761370.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28597..30072
                     /locus_tag="ACHA25_000032"
     CDS             28597..30072
                     /locus_tag="ACHA25_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8761371.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30338..30745
                     /locus_tag="ACHA25_000033"
     CDS             30338..30745
                     /locus_tag="ACHA25_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8761372.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30748..30963
                     /locus_tag="ACHA25_000034"
     CDS             30748..30963
                     /locus_tag="ACHA25_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761373.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30967..31347
                     /locus_tag="ACHA25_000035"
     CDS             30967..31347
                     /locus_tag="ACHA25_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8761374.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31481..31636
                     /locus_tag="ACHA25_000036"
     CDS             31481..31636
                     /locus_tag="ACHA25_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8761375.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31705..32178
                     /locus_tag="ACHA25_000037"
     CDS             31705..32178
                     /locus_tag="ACHA25_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8761376.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32175..33869
                     /locus_tag="ACHA25_000038"
     CDS             32175..33869
                     /locus_tag="ACHA25_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8761377.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33835..34020
                     /locus_tag="ACHA25_000039"
     CDS             33835..34020
                     /locus_tag="ACHA25_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761378.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34024..35199
                     /locus_tag="ACHA25_000040"
     CDS             34024..35199
                     /locus_tag="ACHA25_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8761379.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35192..36715
                     /locus_tag="ACHA25_000041"
     CDS             35192..36715
                     /locus_tag="ACHA25_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8761380.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36771..37055
                     /locus_tag="ACHA25_000042"
     CDS             36771..37055
                     /locus_tag="ACHA25_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8761381.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37042..37377
                     /locus_tag="ACHA25_000043"
     CDS             37042..37377
                     /locus_tag="ACHA25_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8761382.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37672..38298
                     /locus_tag="ACHA25_000044"
     CDS             37672..38298
                     /locus_tag="ACHA25_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761383.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39040..39876
                     /locus_tag="ACHA25_000045"
     CDS             39040..39876
                     /locus_tag="ACHA25_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8761384.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39940..40371
                     /locus_tag="ACHA25_000046"
     CDS             39940..40371
                     /locus_tag="ACHA25_000046"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8761385.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(40433..41755)
                     /locus_tag="ACHA25_000047"
     CDS             complement(40433..41755)
                     /locus_tag="ACHA25_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8761386.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41909..43549)
                     /locus_tag="ACHA25_000048"
     CDS             complement(41909..43549)
                     /locus_tag="ACHA25_000048"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8761387.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43882..44352
                     /locus_tag="ACHA25_000049"
     CDS             43882..44352
                     /locus_tag="ACHA25_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8761388.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44574..45677
                     /locus_tag="ACHA25_000050"
     CDS             44574..45677
                     /locus_tag="ACHA25_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8761389.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45961..46641
                     /locus_tag="ACHA25_000051"
     CDS             45961..46641
                     /locus_tag="ACHA25_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8761390.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46731..47765
                     /locus_tag="ACHA25_000052"
     CDS             46731..47765
                     /locus_tag="ACHA25_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8761391.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47779..48114
                     /locus_tag="ACHA25_000053"
     CDS             47779..48114
                     /locus_tag="ACHA25_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761392.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48098..50578
                     /locus_tag="ACHA25_000054"
     CDS             48098..50578
                     /locus_tag="ACHA25_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761393.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            50634..52193
                     /locus_tag="ACHA25_000055"
     CDS             50634..52193
                     /locus_tag="ACHA25_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8761394.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52190..53554
                     /locus_tag="ACHA25_000056"
     CDS             52190..53554
                     /locus_tag="ACHA25_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8761395.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53725..54795
                     /locus_tag="ACHA25_000057"
     CDS             53725..54795
                     /locus_tag="ACHA25_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8761396.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54810..55478
                     /locus_tag="ACHA25_000058"
     CDS             54810..55478
                     /locus_tag="ACHA25_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8761397.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55498..56076
                     /locus_tag="ACHA25_000059"
     CDS             55498..56076
                     /locus_tag="ACHA25_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8761398.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56477..57388
                     /locus_tag="ACHA25_000060"
     CDS             56477..57388
                     /locus_tag="ACHA25_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8761399.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57766..58683)
                     /gene="rnhC"
                     /locus_tag="ACHA25_000061"
     CDS             complement(57766..58683)
                     /gene="rnhC"
                     /locus_tag="ACHA25_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8761400.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58885..59322
                     /gene="zapA"
                     /locus_tag="ACHA25_000062"
     CDS             58885..59322
                     /gene="zapA"
                     /locus_tag="ACHA25_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8761401.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59380..59928
                     /locus_tag="ACHA25_000063"
     CDS             59380..59928
                     /locus_tag="ACHA25_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8761402.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            60043..62403
                     /locus_tag="ACHA25_000064"
     CDS             60043..62403
                     /locus_tag="ACHA25_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8761403.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62490..62804
                     /gene="trxA"
                     /locus_tag="ACHA25_000065"
     CDS             62490..62804
                     /gene="trxA"
                     /locus_tag="ACHA25_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8761404.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62986..64764
                     /gene="uvrC"
                     /locus_tag="ACHA25_000066"
     CDS             62986..64764
                     /gene="uvrC"
                     /locus_tag="ACHA25_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8761405.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64909..65808
                     /locus_tag="ACHA25_000067"
     CDS             64909..65808
                     /locus_tag="ACHA25_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8761406.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65801..67063
                     /locus_tag="ACHA25_000068"
     CDS             65801..67063
                     /locus_tag="ACHA25_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8761407.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67224..69122
                     /locus_tag="ACHA25_000069"
     CDS             67224..69122
                     /locus_tag="ACHA25_000069"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8761408.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(69452..70171)
                     /locus_tag="ACHA25_000070"
     CDS             complement(69452..70171)
                     /locus_tag="ACHA25_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8761409.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    70361..70408
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            70508..70840
                     /locus_tag="ACHA25_000071"
     CDS             70508..70840
                     /locus_tag="ACHA25_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761410.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70827..72830
                     /locus_tag="ACHA25_000072"
     CDS             70827..72830
                     /locus_tag="ACHA25_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8761411.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72849..73319
                     /locus_tag="ACHA25_000073"
     CDS             72849..73319
                     /locus_tag="ACHA25_000073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8761412.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73340..73924
                     /locus_tag="ACHA25_000074"
     CDS             73340..73924
                     /locus_tag="ACHA25_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8761413.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73939..74925
                     /locus_tag="ACHA25_000075"
     CDS             73939..74925
                     /locus_tag="ACHA25_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8761414.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74915..75226
                     /locus_tag="ACHA25_000076"
     CDS             74915..75226
                     /locus_tag="ACHA25_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8761415.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75248..77029
                     /locus_tag="ACHA25_000077"
     CDS             75248..77029
                     /locus_tag="ACHA25_000077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8761416.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77022..78398
                     /locus_tag="ACHA25_000078"
     CDS             77022..78398
                     /locus_tag="ACHA25_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8761417.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78405..79040
                     /locus_tag="ACHA25_000079"
     CDS             78405..79040
                     /locus_tag="ACHA25_000079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8761418.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79070..80425
                     /locus_tag="ACHA25_000080"
     CDS             79070..80425
                     /locus_tag="ACHA25_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8761419.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80617..82011)
                     /locus_tag="ACHA25_000081"
     CDS             complement(80617..82011)
                     /locus_tag="ACHA25_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8761420.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82103..83074
                     /locus_tag="ACHA25_000082"
     CDS             82103..83074
                     /locus_tag="ACHA25_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8761421.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83071..83853
                     /locus_tag="ACHA25_000083"
     CDS             83071..83853
                     /locus_tag="ACHA25_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8761422.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84022..84393)
                     /locus_tag="ACHA25_000084"
     CDS             complement(84022..84393)
                     /locus_tag="ACHA25_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8761423.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84527..84970
                     /locus_tag="ACHA25_000085"
     CDS             84527..84970
                     /locus_tag="ACHA25_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8761424.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85013..85822)
                     /gene="yidA"
                     /locus_tag="ACHA25_000086"
     CDS             complement(85013..85822)
                     /gene="yidA"
                     /locus_tag="ACHA25_000086"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8761425.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85841..87208)
                     /locus_tag="ACHA25_000087"
     CDS             complement(85841..87208)
                     /locus_tag="ACHA25_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8761426.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87192..88022)
                     /locus_tag="ACHA25_000088"
     CDS             complement(87192..88022)
                     /locus_tag="ACHA25_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8761427.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88185..88622
                     /locus_tag="ACHA25_000089"
     CDS             88185..88622
                     /locus_tag="ACHA25_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8761428.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88698..89318
                     /gene="rpoE"
                     /locus_tag="ACHA25_000090"
     CDS             88698..89318
                     /gene="rpoE"
                     /locus_tag="ACHA25_000090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8761429.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        29444 a        15120 c        18426 g        26403 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaaa
     3301 tattttttta tataaatatt tgtgttacta attccgttaa tatacataag tgaaagcgtt
     3361 ttatataaga acttgaaaag aaagaaggga tagacatgaa aaaaagatac aaattggtga
     3421 tagctttgct aaacattacc ctattattca gcttaagtgc atgtggaaaa actaacgaag
     3481 aagtaacagc aaacatcgca gatccaatga taaaaaaagc cactacatta gaagtatatg
     3541 tcgcaaacgg caaactgaca aactatgttg aaaatgctgc aaaaatctat aaccgcaaaa
     3601 ctggagcaaa tattgactta aaaattgtga atgtagcttc gggaacagca acagtacaaa
     3661 tgatcacccc aaaattggta acacacgaag atatgccgga tattatctcc atggcagaca
     3721 cttcagcagc aggtgtgctg gaaaaatttc aagatgcttt ttatagtgct gatgaatatg
     3781 gattttatga caaatatggc gatcagtttt atgatcaaaa attgaatatt ttgcgtcaac
     3841 aaacgagtaa tgaaacgatt attccttggg ctagtgattt taccccagca ctttgttatt
     3901 atcaaccaaa aaaatttgct gaaataggtg tagattttga agaaatcagt tcttgggaag
     3961 aatatatcca ggtggcaaaa gcattaaaag aaaaaactgg tagttttgga attgccttgc
     4021 cagaatctgg ggaccaagaa ttgtttatca acatgctagc ccagcagaat caatctttgc
     4081 ttgatgaaga aggaaatatc cgtctgaata ctaaagaagc gaaacatgct gcaaagttaa
     4141 tcaaagaaat gatcgactca gatattgtac atttctacgg tgcacaagat gctgagaaag
     4201 catttcaaga atctgcaatg tttgttgctg gcggctggta tgctacgaat atgtctttga
     4261 attttcctga tgcatctgac aaatggcgca tggctccact gattcctttt tcaaaagaaa
     4321 atccaggaaa aggtgcagta tcaggaggat caagttttta tgtccctaaa gatgcaaaaa
     4381 atccacaagt ggcacaacaa tttcttacat ttatgctgac agatgaagaa tgcttagcca
     4441 acgcactgga gctgggcgtg gctacatcta actcattggc ctatcaaaca gaagcagctg
     4501 agaaaaagtt tgactactaa ggaggacaaa aatactatga actattgaat aaaatcaatg
     4561 aaaatacatc aaatactgtt ttaccagcca cttattcaga tgctacgtct tatttgacta
     4621 ccgctgcata taactattgg caaacaggtg attttgaaaa ttcatatcta aaagaagcag
     4681 aaaattttgc tcataagtac ggcgtaaaat taactggtga ttaaaaaata ttaggaggac
     4741 ttaacgatga tgcaatcttc gttaccaagc aagacaataa agaacagcaa gaaaaaagca
     4801 acagatacgt ctcataaaaa aagtgcttat ggattggtca caccttttgt tattttcttt
     4861 attgtgacga ttttgattcc catcattatc tcgtttgctt ttagttttat gaaaattggc
     4921 tactcatttg aatttgttgg atttgacaat tatctagcta tcttccgaga tccattgttt
     4981 attaaatctt acacgaatgt tttgattatc atggcaggat caattccaat aacgatgcta
     5041 ttagctattt actttgctgt tttattgaat tcagcacagt taaaaggaaa aggatttttt
     5101 agaactgtct attatattcc aaccattaca tcaacggtgg ctgtagcttc cgtattcatg
     5161 acttttttca atccgactgg gttattcaat ggatttttga atgcgctagg aattaacggt
     5221 gtacagtggt taacaaatcc tttttggata cgaatatcaa tgatcatcac gatgatttgg
     5281 cttagtgtag gatataacac tattttattt ttagctggtc ttcaagggat ttctaaagag
     5341 atatacgaaa gtgctgaaat agatggagca tcgaaatttc ggcaattctt tttattaacg
     5401 atccccatgc taaagccaat cattttaatg gccacagttt tagccactat caatggcttg
     5461 ggtaatttca atatcccaaa tatattcttt gggacttcca atggaccaga aaattcagct
     5521 ttggtcgtag gagtcaattt atataaaaca agttttgaaa tggttgattt tggaaaagcc
     5581 tctgctattg cctggacgat ggtgtttgta tctgttatat tatcggctat tcaatttaaa
     5641 ttcggaggtg ataaaaatga ctaaacaaaa gatacaaagc agaattttta cgtacgtcat
     5701 tattgtgttt ggtgccttaa tatttttgat gccatattta tatatgattg tagcttcaac
     5761 gcaaagtaat caagaaattt taagtggcgg aatcaatttc aagtttgggg atatgctatt
     5821 aaaaaactgg caagccatca tggcgagata taattatccc ttgattattt ggaattcgat
     5881 tgttattgct gtaatcagta cagttttagc tacagccagt tctacaatgg cgggttatgc
     5941 attagcaaag tatcgtttta tggggaataa actgttgttc aatacagtta tgctatctag
     6001 gatggtgcct ttttttacca cattgattcc tttattttac attatgagtc gtttaggatt
     6061 atcgaatact tatgcaggtg tgatcatccc ttcgattgcc agtacaactt cagtgtttat
     6121 gatgcgacaa tatagcagtc aatttcctac agcactgatg gaggctgcca gaatcgatgg
     6181 tgccaatgag tgggtgattt ttacgaaaat cgctgtaccg attttatcac ctacgattat
     6241 tactagtggt ttacttatct ttatgggaag ttggaatcaa tatctatttc cattagtcat
     6301 gctttcagat aaagataaat atacggttcc cttaattatc aaaaatatga cggtttctag
     6361 tacaggtgag ccaattaatt atggcgcaat tatgctaatt ttagcaactt cagtgatccc
     6421 aatggtcttg atttatgtgt gggcgcagtc taaatttaag gagaatgaca ttggagctgc
     6481 aaataagtaa aaaaaataac gaatgttaac catctagttc gttctaggag caactccaca
     6541 ttcataaaaa ttaaaagaat ggcgggatat ccatgaatga acagacgaag gaatggtcaa
     6601 gaaaaatttt gaatgtagct tctgaaaaga tagatgcgga attagacaga atggggaata
     6661 aaattccata tatttcttac aacggaaaat atacagacgt aataaaggac agaggaatcg
     6721 actggtggac caatggtttt tggagtggta ttttatggca gctctttcac aagacaaaac
     6781 gagaaaagtt caaagaggca gcaatacaac aagaacaatt attagaatct gccctgtacc
     6841 atttttcaga cgttcatcat gatgtaggtt ttttattctt accatcggct attgcacatt
     6901 atcaacagtc tcaaaacaaa catagtcgac aactagggtt acatgcagct acggtacttg
     6961 ctggacgcta taatatttca gggaactatt tagtagcatg gaatgaccat aaacctggtt
     7021 ggatgattat tgatagtatg atgaatttac cattactgta ttgggcttcg agggaaactg
     7081 gtgatccgag gttctctaaa attgctcggc agcatgcaga tacaacagct aagtatctag
     7141 tgaggaaaga tggatcaacc gggcatatcg cagaatttga tgcagaatca gggaaatata
     7201 ttcggttact tggcgggcaa ggatacaatg agaattcttg ttggagcaga ggaaatgcat
     7261 gggctttata tggattcgcg ttaagttatc gttacacaaa aaatccgtac tatctagcta
     7321 tagcagaaaa gatagcagta aattttattc agaaaatcga aaaaactggt tatattcctc
     7381 tcattgattt tgctgtaaaa gattcgaata ttgataccag tgctggagta tgcgctgcat
     7441 gcggtcttct ggaattagca ggatatgttt cctctgtacg atcaaaaatg tatcatgaag
     7501 ctgctatcaa tatactccaa aagacagaaa agcactatgc aaattggaat atggatgaag
     7561 atgggatcat tggaggcgga gcagaagcat accatagaga actgacttat aatgtgccac
     7621 tgatctattc tgattatttt tttattgaag gcttacttcg attagaacag tcggaattag
     7681 ctatttggta gtaaaaagag gagaaaatac aatgaaacat ccagtaccac tattaaggac
     7741 cagacaggat tactttgact tatttaaaaa gataaatcaa ccactccagc cgttttatcg
     7801 agcaagtggc gcacaaattg attatataca tcaaggggta ggatatggaa ataaaattgc
     7861 aggaatggaa ggattctctc gggttttatg gggagcgggg cccgcaattg accaattaga
     7921 tgaacgatgg ttgaaattaa ttctatcagg aataaaagct ggaacagatc cttcccatcc
     7981 cgattattgg ggagaaatac atgaaagaga ccagcgtatg gtagaaatgc ccgcaatgtt
     8041 attagcgtta tatcatcatg aacaattatt atggaaaaaa ttagatacag gagaaaaaag
     8101 ccagattatt cattggttct cgcaaatttt tgaatatgaa tgtgcagatg gtaattggca
     8161 attttttaag ataattgtcg gaacaattat ccaaaaattt ggttatccag ttaaagaatc
     8221 agcactgaaa gaagcatttg ataatataga aaattgttat cttcaaaatg gctggtatcg
     8281 agacagttca agaggtcgcc aagattatta taatcctttt gcatttcatt attatggttt
     8341 gatttatagc gtactagaac cagaaaagcc cattagccag atatataaga accgagctat
     8401 tgatttttct agagactata ttcatttttt tgcggaagat ggtgcaaatg tgccttttgg
     8461 tcgaagcatg atttatcgtt ttgcagtttc aagcttttgg tcagctatgt tgtgggccga
     8521 tcttttgcct tttaaattag gtgaaatcaa ggggttagtc aatcggaata taagatggtg
     8581 gatgcaaaaa gaaatatttg atgaaaaagg tatcctgaat attggttata cctaccctca
     8641 attgacattg actgaaccct ataatagtac gttatctcca ttatggctga ataagatttt
     8701 tttattgttg gcgctacctg aagaccatga gtattggacg gctaaagaaa tgcctatgcc
     8761 tattcgtgaa ggaactaaac ttctttcaga agccaatttg ctcatacaac atgataatgg
     8821 acatacattt tttttgaatg cgggacaatt agggccaaat ttccatacat tgaccaatga
     8881 aaaatattta aaatttgctt attctagtca atttggtttt tcgataccgc gagcgaatca
     8941 attgaaagca gaaatggcaa tggattcaat gctagggatt cagcgagcgg acacatatat
     9001 cacgaccagt tttaagaatg agagcatcaa aacctatgga cagttcatcg ttcgaaataa
     9061 tgtacgagac attgtatgta agaaagaggt acttgcctct acatgggaac ctactttaca
     9121 ggcaaaaatc agaacttggc ttattccttt tgaaggctgg cagattcgcg tacacaaagt
     9181 agaattagat acagaatatg ttctatatga gacagggttt gctattgaat gttctcctga
     9241 gaaagaagga ataatcatag aagaagacaa ggaaaactat agggtgagtg aagctggctt
     9301 ttcaggtatt atctgtttaa gtgacgatac tattaaacga aaatccacag ctattatggg
     9361 tttgcccaat acaaacttga tgacatggga aaacactttt attcctggat tagagggtac
     9421 atttggaaaa ggaacccatt ggctcggaac aggagtggtt gctcatcaag atcgggatta
     9481 tagtttagaa gtatggaaca atcgtcccaa aattgatttt gatgggacaa ccgggctaac
     9541 tatccaaaat aaaaacaata aaaaaagact aaaattatgc taaaggctgg tggggaaatg
     9601 cttgataaag tgatgtttga agagataata catccagaaa aatggataaa tgaatgttat
     9661 aaagaattaa tggaaaaaga atggaataat gagatcgagc gttgtcgcag acacacggag
     9721 aggttaatca aacgtatcag taacagtcag tatcaattcc caagcgagtg tcctataaaa
     9781 ggacgttatc cattaagaga caatattggt tggacagaag gattttggac aggaattaat
     9841 tggttgttat ttgagaagac gggtgatcct ttatttcaac aaacagctga aaaagcagat
     9901 atagatttta tccacaggat tgactgtcaa attatcacta atcatcacga tcttggtttt
     9961 ttgtattctt tatcaaccgt agcagattat aaaataacgg gaaatcgaaa aatgaaggaa
    10021 gcttcaattg atgctgcaga aacattactt cagcgttttt gggagaaggc aggagttata
    10081 caagcttggg gagatcctag tgatccttca caacaaggta gaatgatcat ggattgttgc
    10141 atgaatcttc cattattgta ttgggcgagt gaagaaactg gaaatcaaaa atataaaaaa
    10201 gctgctcagg ctcatattca aaatgcagca gattatttag taagagaaga tggttcaact
    10261 tatcatacat tttatatgga tgtcaaagat ggaaaaccaa gatatggaag tacgcatcaa
    10321 ggttatagtg ataattcctg ttgggctaga ggccaagcat gggggattta cggttttgca
    10381 ttaagttatc gttatttgaa agaacaaaaa tattttgatt taagcaaaat attagccaat
    10441 tattttatga atcggttgcc agaagatacc atttgttatt gggatttgat ctttgatgat
    10501 tctagcagag aagaaaaaga tacatctagt atggtgatca tggtttgtgg attattagaa
    10561 ttaatagaat caggtctatt agacaaaaca gaaaagaact tataccagtc tgctgtttgg
    10621 aaaattatgc gacaattgaa agaaagatat gctacaacgg aagaagaaga tggcttttta
    10681 acacagtctg tttataataa gcccaaaaat aatggggtaa atacaccttg tatttggggt
    10741 gattactatt atttagaagc tttagtgaga atgactagta attggtcttc ttattggtga
    10801 aaaaggtaaa tatggaattt ttgtttgata aaataaaaaa agaaaaacca aaacagcatg
    10861 aatggtttct gttttggttt tttcgttaat tttcatattc ttctaattcc aagcttagtt
    10921 cttcccattc cgtcaatttt gtttcttgat cttgtctagc agcttctaaa tcttggttca
    10981 gctcattgag ttttacgtga tcttccaggt tttctggcgc gaccatttct tgttccaact
    11041 gatcgatcca ttcatcgatc cgcgccattt cttcttcaac ctgagtaatt tttcgttgaa
    11101 ggctacggat aagcttttgc tgttctttgc tttggtaata atcagatttg ttcgtgggag
    11161 caggagcaga ttctggctca tcttgtgcca acaattcggc gatttcttct tcttcctgct
    11221 ttttctctaa ataataatca tagtctccta gatagacttt gctgccattt tcagagagtt
    11281 cgacgacttt tgtcgcgatc cgattgatga aatatcggtc atgggaaaca aaaagcagtg
    11341 tgccgtcata atcgatcaat gcattttcta atacttcttt gttgtcaata tctaaatggt
    11401 ttgttggttc atccaaaatc aaaaaatttt ctttgttcat cgctaacttg gctaaagcaa
    11461 cccgtgcttt ttcaccgcca cttagcagag ggattggttt ttctacatct tcgccagaaa
    11521 ataagaaagc acctaaagca cttcggatct ctttttctgg agtggtagga tgctcatccc
    11581 acaattctgc taggatcgtc ttgtttgtat gcagatcagc ttgaccttga tcgtaatagc
    11641 cgatggtgac atttgtccct agcgtttctt ttccacggat aaaagggatc tggtgaatga
    11701 ttgatttcag caacgtcgat ttcccgatac cattcggacc tactagagca atcgcttctt
    11761 gacgacggat atctagattg attggttcag ataaggtgac attatcatat ccgatagcgg
    11821 catcttcgat ttgcaagact acatttccag aaggcttttc tatactgaat agaaagtttg
    11881 cttgtttttc atccccttga ggacgatcca gccggtccat tttttctaat gtctttctgc
    11941 ggctttgcgc tcgttttgtg gtagaggcgc gaacaaggtt ccgagcaacg aagtcttcta
    12001 atttatggat ttctgtttgc tgcttttcat aagccttcca ttcacttgct aactgttctg
    12061 ctttcaaatc tagatatttc gaatagttac ctttgtaatg gcgcattttt cttctgctta
    12121 attcatagac ttcattgact actttatcta aaaaataacg gtcatgggaa acaatcaata
    12181 atgcaccagt atagctttgc agataattct caagccagga aagtgtctcg atatctaaat
    12241 ggttcgttgg ttcatctaga atcaaaatgt cagggtgttg caaaagcatg cgagctaaag
    12301 ctaaacgtgt gcgctgtccg cctgataaag tatcgatttt ctgatcataa aatgaagaat
    12361 cgaatttaaa cccatgcaat acggaacgaa tttcattttc ataaccatag ccgtttcgat
    12421 ctgaaaaatc gtgttgcagc cggtcgtatt cttttaaata agaactataa gcaggtgtgt
    12481 ctggcgtacc ttcgctaatg aaaatctcta gctcgcgcat ccgctcttcc atttgtctaa
    12541 cttgttcaaa agctttcagc atctcgttcc aaactgtttc attggaatca agtcctgtgt
    12601 cctgagcgag gtatccgaga gtcgctgttt tatttttagc gatctttcct tcatcaggtg
    12661 cttcaattcc agcaatgatc tttaataaag tcgattttcc agcgccattt ctgccaacta
    12721 aagcgacacg agcgcctgta ctgatttcta aatgtatgtt ttcaaataag acttctgcac
    12781 caaaatggcg agcaatctga ttggcttgta ataatatcat ggaatcctct tttctacttt
    12841 gtgaattctc ctcaaataaa aaactatttt tcaattagta gtctaccata aattccatta
    12901 tactgcgaga tttgcagaaa tgataaataa atgaataaag aataaaattt cacaatatct
    12961 gagcaatgga attgattttt tcctataaat actgctatga tggagaaatc aaagatgtaa
    13021 ttagacaata cgtggaggtc atttcgtgaa agatcatatt attccaaagg cgacagctaa
    13081 aagactgcct ttgtattatc gctatttaag gattttaaat aatgctggaa agaaaaaagt
    13141 gtcttcaact gaattaagtg aagccgttca agtagatagt gcaacgatcc gtcgagattt
    13201 ttcttatttt ggcgaattag gaaaacgtgg gtatggttat gatgtagaag atcttatgca
    13261 cttttttgca aagacattaa acgacgatca attgacaaat gttgcgctga tcggtgtagg
    13321 gaacttggga agtgctttac taaaatacaa attccatcaa agtaatagca tacgtatcag
    13381 ttgtgctttt gatgtgaatg aggaaatcgt tgggcgcatc gtagacggta tccccgttta
    13441 tccaatggaa gatatgtcag aacaaatcaa gatccaacaa atcgaagtag caattttaac
    13501 gatacccgct gaaagagcac aagaagtagt caatcagtta acggaagcag gaatcaaagg
    13561 aatcttgaac tttaccgctg ctcgattgaa tgctccgtca gatgtattga tccaaaatgt
    13621 tgatttgacg aatgaattgc aaaccttgat ctatttcttg cattctgacg caaatgcgca
    13681 ttccgaaaat acagcagaat aaaaataaaa agcgattgaa caaatgaagg caaaactttc
    13741 acttgttcaa tcgctttttt ctgtttggac aacgattatt ttgttcggaa gatttcttca
    13801 taagaaaggg aatcatcttc tgctgtcagt tcatctagta gataacggat ttttttaaag
    13861 tcgatgaacc gtgcttgccg aaagacttct tttccttgat gaaaaatcag gacaactggc
    13921 gcagtcagca cttcaaattt actagctact tctggtgttt caaatgcatc caaatgaagt
    13981 gccggaatat cataatgtgt gagaagttcc tctagtcgtg gacgaacagc gtgacaaaca
    14041 ctgcaattag gcatcgaaag ataaatgacg gcaacggatg actgttgcag tatgtcttga
    14101 acttcagtca agttagtggt ttgtttcatt tttattctcc cttttatgat agaagtgata
    14161 tgtatgagac cttaatttaa tgggaataac agaaaaagtc aattgaagtg ctttggtaaa
    14221 aaataagagt tgtgtaaaaa tttacagagc aacttgtttt ttttgaataa aagcgtatat
    14281 ttgcaaagcg aatgaactag aaataaaaag gaggaaactc atgttttctt ttattccaaa
    14341 ttcggttttg cagatgagta cgattttctt atcgatcgtt attgaagcat tacctttcgt
    14401 attattaggc tgtcttatct caggcgcctt acaagtattt ttgaccccag aacgtgtgaa
    14461 gagatggctt cctgaaaatc gtatcttctc tatttttact ggttcgatct taggattttt
    14521 ctttccttca tgtgaatgcg gcatcgtacc gatcgttcat caatttgtta aaaaaggcgt
    14581 accagttcac acagcgtttg cttttatgct gactgctccg atcatcaatc caatcgtgat
    14641 tttttccact tttatcgcat ttggcaattc ttggcagatg gcaagctggc gggtgatagg
    14701 cagctttttt gttgcccttg tgattggtgt ctggcttgcc tattttcaaa aagacgctgt
    14761 attgaaagaa aaaatcacgt ctaaagtagc agaaaatcac catcatcatg tccatgaaga
    14821 agaacaaagt gagaagaatg aaccaaaaaa tcagctgatc catcaaactg ggcatgtgtt
    14881 gacacattca atcgacgaat tttttgatac aggtcgttat ttgatcattg gcggattaat
    14941 cgcagctgcg atgcagactt atttaccaac tagaattatg ctaacgctcg gctcaacaaa
    15001 gattttagcc attatcatca tgctgttatt ggcctttacg atgtctcttt gctcagaagc
    15061 agatgcgttt ataggatctt ctttgttaag tctgttcggc acagcacctg tagctgcctt
    15121 tttggtcttt ggcccaatgg tcgatatcaa aaatctactg atgatgaaac gatattttac
    15181 aggacgcttt attttatcgc tgatcggact gattgccctt agcgttattg gattcacatt
    15241 atttatttag gagggaaaat atgctgcggt ttttgatttt atctgggtat ttcgaattga
    15301 tgatgtactt gcaagtttct ggcaaactcg atcaatatat caatgtccat tatcgctact
    15361 tagctattct ttcaatgatt ttatctgctc ttttggcttt agttcagctg tatttgtggg
    15421 tcaaaaacgg aaaagcagaa gaaaaggcac acgagcacga tcatgaccat gggttgtcaa
    15481 aaccttatca acgtggaatc gcttatgtac tgctagcttt gccagtaatc gtggggacgt
    15541 tgtttccaac agttagtctc gacactacga tcgtagaagc caaaggattt aatttcccag
    15601 taagtaaaga atcagtaggg gatccggaga tgcagacaca atacttaaag ccggatacca
    15661 gtatttattt caacaaatca gactacttag accaaatgaa tgagttgaaa gaaaagtatg
    15721 gggataagca aactatcaag atcacagatg aaaactattt agaagttatg gagctaattt
    15781 ataattaccc tagcgaattc attggcaaaa aaatcagcta tgaaggattt gtttaccaaa
    15841 caccgaacga tagcaataca gatatcttct tgttccgatt cggcattatc cattgtgtgg
    15901 cagattcagg agtctttggt ttgttgacgc atatgccgga aggagtaacg gttaaaaatg
    15961 atgaatggta taaaatagaa ggaaccatcc aatcagacta ttatcaacca ttcaagcgtg
    16021 aaatcccatc tgtagttgtt acaaacgctg agaaagtctc tgctccgaaa aatcagtatg
    16081 tgtatcgttc tttttaaaag aacaaaataa ataaacatag ctcagacagc tgacacgcaa
    16141 ggacgtcggt tgtctggctt tttttgcatg atgtattcat ttgtttgata tttattttag
    16201 aagcagcatc ctgaaagtat ttgatatcgg tttcaaaggt gttggaaaag attgtgaaga
    16261 gaattacaat ttattcctaa catgtatgtt gaaaagttgt atgaaatgat tttgatagta
    16321 aaatatcgag tggaggtact ttttatggta acgcgtatat ttattttgga tgatagtttg
    16381 gtttttgaaa aaatgctagt agaaattttg gaagaatcca gaattttatt aagtccttgg
    16441 aaatttgaga tcgttcaggg attgaacgtt atacaatttg ttgaattcat taaaaataat
    16501 gaaatctatt cctcagatat ttttttccta gacatcgatt tgaacgccta ttatagcggt
    16561 atcgaactag gcgaaatgct tagagaaaag aatgaaaagt gtaaaattgt ttatctaacg
    16621 tctcatccga ataaagcaat aactgttatt aatcgaaaga ttacgcctag tggttatttg
    16681 ataaaagaat tcacttatga aaaaaataaa aaaagcatcc aaagttactt attgaaagaa
    16741 aaagaaaact atcctgttca acatgctacg aaaaaaaggt ggacaactgt ccgttatggt
    16801 aatagcgatc actatatcca ttacgatgaa atcctttatc taatgagcat gccaggttat
    16861 aaaaataaaa taggcctagt attgaaagat gaagagattc tcgtaaacgg cacaattaaa
    16921 aaatataaaa aatcactcga atatgagtat ttatgtaaag atttaaaagc ttatatcatt
    16981 aatactgaaa acattacatc tattagcaga gtaaacgaaa tagtaacatt tgcaaatgga
    17041 gaacaactag agatgggaaa gagaagtatt gataaactaa agaattttat gcaagaaaag
    17101 atagaaaatg aatcatttta aagaaaacaa agactcatat cttacctgtt agaagagtgt
    17161 tttcctgtag aagagaacaa cccaagtagc agcctagtcg tttctatcct tttttgttat
    17221 gcactctttt tgacatttac tccaccaata acgaagggta caataaaaag taataacaaa
    17281 caaaaggagt acagaaatga ttgaattcat caatgtaagt aaaagttacg ggaaaaaaca
    17341 agcattaaaa gacttgtctc tctcgataag acaaggggaa atttttggat ttttaggaca
    17401 taatggtgcc ggaaaatcaa ctacgattaa aagtttagtt agtattattg aaccatcatc
    17461 gggaacgatt ttagtagatg gaatgaaatt gacggaaaat cgattgagta tcaaacaaaa
    17521 aatcggttat gtgcctgatt ccccagatat ttttttacaa ttgacggctg gagaatattg
    17581 ggacttgatt tcagctgcct atgaattaga cacacaaaaa aaagaaaagc gtttagcaga
    17641 attgacagcg ctttttgata tgtatagtca tcaaaatgaa acaatcgcaa gtttttcaca
    17701 tggaatgcgt caaaaaacga ttttgatcgg cacgttgctg ccagatccag atatttgggt
    17761 attggatgaa cctttgcaag gacttgatcc tcaagcagca tttgatttga aagaaatgat
    17821 gaaggctcat gccgcaaaag gaaaaacagt cattttttct acacatgtat tggataccgc
    17881 acaacagtta tgcgataaac tggctatttt gaagaaaggt gaattgattt atcagggggc
    17941 agttagtgaa ttattgaatg gctcacctga tgaaacgcta gaaaagattt atttgaaaat
    18001 ggctggaagg caagcgacag gagatggacc gtatgtataa gcgacaatta ttagaattaa
    18061 ttcgagttaa tcttttatat gttaatccac agctcacgaa taaagcaaga gagaaaggaa
    18121 aaaagggaaa gcgattaact atctatcttt tgtctcaaaa tcttttttca gggctgattt
    18181 ttttactgat ttacggattt gctatgtttg ctatgaactt tgttaaattg ccaggtttct
    18241 ttacttatta cgtggcactt tttggtatgt taggtttaac tcaaagtata tcagtcattt
    18301 ataatatctt ttttgaagga aatgacctgc aagtattttt gcctctgcct tttggacaag
    18361 aacaggtttt ttcagcaaag attttggtag ttgctttgac catcattcct tttgtttttc
    18421 cgcttctagt catgtttatt ttaaccggat ggcgagctga tctagggatt ttgcttacta
    18481 tcattatctc aatccttttg ttttctctat ttttgattat tcttttttgt ctggctagtt
    18541 tgattgtttt tggtttggct cgaacagcag tgtttaaaaa acatagaaaa acagtttcta
    18601 gtatcttgct tggcagctcg atgctgatcg catttgctgg aatcatgtgg atgaatcatc
    18661 aaacggggac tattgaaaat gagttaacag atcggcatcc gatttcgata ttattgccat
    18721 tctatgatgc tgccagtcag ccactttctc aacaaggttt attgggttgg ggaatactag
    18781 ctatcacggc tctgggattt ttccttgctg taaaacagct gatagtacca aagcttacag
    18841 agcaactgac tgattcaaac agtgagttca aaataaaaag aacccataaa aagaaccaaa
    18901 atgtgcatca gcttttattt agttacaatg ctcaattagt aaaagaacct aatttgatta
    18961 tgcaagttct ttcttcttcc ttgttaactc caatcatttt tattcttgct tttgccctag
    19021 gaggcgaaat aaaattaact gatttagata ataagttaat cggtgttgtt ttcctggtag
    19081 ggatcgcatt ggctttctta acagttaatc agacttcttt tatcagcaat cttatttctt
    19141 tggatcaaga aaattttctg tttatccgtt ctttgccaat ctctatgaac caatatttaa
    19201 aagaaaagtt cagattcgga tggatattgc agagtttttt aactggtggg atcgcattat
    19261 taagcggact gtcttttcag ctgcctttgt tttttatccc tagcttgcta tttggttctt
    19321 tattcggctg ctacttattg tcactacgtt atttcgctag agattatcgg ttgctgctgc
    19381 tgaactggac caatattaac caattattta acagaggttc aggaagtttg ggtttagtcg
    19441 caactatgat gggttcattg atcatcagta ttattcttat cgtcttatac ggaatgttgg
    19501 ctttgtttta tccattttgg ttagtgaata ttcctgtgat cggtttagtt ctaatacttt
    19561 ccgctctatg gatcgtttat tatcaacgaa cgttttggaa aaaatttgaa taaagaagag
    19621 gggccgtgac aaaaatcatg tcgcagcccc ttttttacag atagagggtg ttcaaaagtc
    19681 agtttctcag tattaagtca attttatgta aaaatgtgag aagctatttt tacaaaactt
    19741 tttttaatac tcataattac ttcttattgc tcctgcggtt acttgtcgca atgtttgtga
    19801 gatattttgt catcgctcaa ggctgaacgt ctctttcaca accttttctt tgttacactt
    19861 cgtttattta aaaagccgtt tttttcgttt catcatgatt gcgtcacgct ttactttgaa
    19921 attacggatg ttatggttaa agacttctga taatacaaga cctaaagcga ttgctccagc
    19981 aatcataatt gcttggaccg cataatgagc cgcttctcga taggattcag taacgagatt
    20041 tcgaaccgct tgataagcta aaccaccagg gactagagga accatcccgg gaatattgaa
    20101 aatcgttacg ggcattttca aacgtttaga gaaaagatcg ctgatgaatg ctactcctaa
    20161 agaaccaagc aatgaaccaa aagcagcaga tccgcctatt tgaacacaga tccagtagat
    20221 catccagcca aaagcaccag aaagaccaca acaaatcaat gaacgcctag gtacatttgt
    20281 aataacggca aatgccgcag tcgctaaaaa actaaaagaa aactgaataa atatcgtccc
    20341 catacttgtg ttcctttcct aataaaaaag ctggaaaata aaagcaatcg caaaaccgat
    20401 catcgtagca gtcatcatcg cttctgttcc gcgagaaact cctgagacat aatgtccagc
    20461 tagcaaatca cggatagcgt tcgtaatttg aactccagga actaaaggca tcacacatcc
    20521 gataatgatt agatcctgat tgcttcctaa gccaaaacgt acgctgtaga tagcggctat
    20581 gccaatcaat aaagaagata aaaattccga taaaaatttg attctaaaaa atttcaaact
    20641 atatagataa gctgcgtatc ccaatcctcc aatgactaaa gtcacaggta aatcggtcat
    20701 ctttccgcca aataacacca taattgttcc actgatcact gccgcgctaa cgatttgcaa
    20761 ccagacagga aagaaagaac ggtctcggtc gattgcttgc agttcgcagt aaagttcttc
    20821 taatgttagc tctgaagcaa catatttacg tgaaagatca tttactttta ctactttttc
    20881 taaattaatc gaacggttgg taatttgttc cattcggatg gtcgaggtac gatcaagacc
    20941 aataaagaga ccggtttgag tgacatagct gaccaaacgg aaatttccag aagctaaagc
    21001 aattcgactc atcgtatctt ctacccgata catctccgca ttgctttcca tcatgatttt
    21061 cccagcgagt aaacaagtgt ccagcaacag atctaattct tttcccgtca tagcatcttc
    21121 tccaataatt gattgattag ttttatttta ctacaaaaca taccagaagc gtaaagaata
    21181 aaaaaacttg aggaaggaat aaaagaggcg tatactgttt ttgtattgcg ggtgtagttt
    21241 agtggtaaaa tcccagcttc ccaagctgat gtcgcgggtt cgattcccgt cacccgctta
    21301 aaataggttg tgaaaaagct gctctgcaac aagtaataag aacagccaag caaaaacaac
    21361 ttcttatgtt tttaacttgg atggacttat taccgcagat gcaagctttt gcgcaccgtt
    21421 taccaagcgg ttgtgaaatc agtgtattgt cttgagtatg aagaaaattt ttgaataata
    21481 ataaatacct tacagataga gtggtgactg attagagacg aggaatccgt ctcttttttg
    21541 tatttttaag ccatcttaaa acaggtttta ttttttgctg tttctatttc tttctagtat
    21601 ttatttatag gggaacatat gttaagattt agggagctgt tagtaaagga gtaaagaaat
    21661 gagcaaaaaa acaaccaagg cgatcgtggg gatcgtcgct gctgtgattg caggggcatt
    21721 cggtgtttcc accacacaaa aagattcaga tatctttcag cagattacaa ctatttttga
    21781 ttccacacca caagcttctc aagtagcgga tgtgccagaa tatgatggaa aacatcaaga
    21841 aatagaaatc aatgggaatc agccggaatt tacaaaagag gatcttagcc tttccaaagg
    21901 aacatgggaa agctattcaa atattgataa attgaacaga gtaggacaag ccaatgctat
    21961 gcttggaaaa gatttgttcc ctactgaaaa gagagagcct ttgtatattg acccgacagg
    22021 ttggaaacaa aagaaattaa gtgacgggca atggttatat aatcgcagcc atttaattgg
    22081 tttccagctg actggtcaaa ataacaacat taaaaacttg atgacaggga cacgttcgtt
    22141 gaatgctcca tacatgcttg ctcacgaaaa tgatattgcg gcatatatca aagaaacaaa
    22201 tcatcatgta cgctaccgtg tcactcctca ttttgagggg aatgaactgg tagccagggg
    22261 agtgcaattg gaagcagaaa gcattgaaga caaaaaaatt gaattcaatg tctttatcta
    22321 caatgttcaa gatggctaca cgatcaatta tgagacagga caagcaacaa aaacaaaaaa
    22381 ttagtagcag ctattcgaat tttttatgaa tattatgaaa aaattgtgta aaaacataca
    22441 ttcaaagaaa tatagcgtat actttatgaa aagagtactt ttgataaagc tacagaaaaa
    22501 aggaaaaaaa catacaaata gtgtttctta atttattcgg aagtttggaa tatttgtttg
    22561 tagttttccc tttcttcgag taaaataagg ttgtagaagg taagctggaa ttaccttgcg
    22621 ggaaagaggt gtagaatcat gggttttaca gatgaaaccg tacgttttgg ttttgacgat
    22681 agtcgcaaaa aagaagtgag cgaaacatta gcgattgttt atcgggcttt ggaagaaaaa
    22741 ggctacaatc caatcaatca gattgtcggc tacttgcttt ctggagaccc gtcctacatt
    22801 cctcgttatc gtgatgctcg aaatttgatt cgccgtcacg aacgcgatga gatcatggaa
    22861 gtaattgtca aggactacct ctcaaatcat ggagtgaatt tatgagagta atgggcttgg
    22921 atgtcggttc taagacagta ggtgttgcta tcagtgaccc ctttggctgg acagctcaag
    22981 gagtagagat cattcggatc aatgaagcag aagaagagtt tggctttgat cggttgaagg
    23041 aattagtagc acaatatgaa gtagaccggt ttgtagttgg attacctaaa aatatgaaca
    23101 attccatcgg accaagggca gaagcttcca tgtcatacgg agaaaagatc aaagaactat
    23161 tctccttgcc agttgattat caagatgaac gattaaccac tgttcaggca gaacgtatgc
    23221 tagtagagca ggcaaatact tcaagagcca aacgaaaaaa agtcatcgat aaattagcag
    23281 ctgtgatgat cttacaaaat tatttagacg gctcaggtaa gcaattattt taataataaa
    23341 agaaaagagg taatcttatg acagaacata accattccca cgaccatgaa ggacacgaac
    23401 acatcacatt ggtggatgac caaggaaatg aaacattata tgaaatccta ctaacagttg
    23461 acggacaaga agaatttgga cgtaactatg ttcttttata tcctgctggt gtttcagaag
    23521 atgaagatgt agaattacaa gcttatgcat acatcgaaaa cgaagatgga acagaaggcg
    23581 agttagaaca aatcgaaaca gacgcagaat gggacatgat tgaagaagta ttcaatacat
    23641 tcatggcgga agaagaagaa tagactataa agctaggaga aaccttgata tgatagggtt
    23701 tctccttttt ttatttttta ctgtatgcca tattgtatgc catctagtca atatttaagt
    23761 gttgtactaa tttttcaaca gtcttttctt tcgtttcttt tgccaagtga ccgtaacaat
    23821 cgatcgtctg actaattttt gtatgccgta atctagtttg aacttctacc atagtagctc
    23881 cagcgtccaa gagcattgaa gcatgagtgt gtcttgctac atgcacaccg agaaatggaa
    23941 ccatagcttt ttcacaaata gtaatcaatc ttgcataaat agcgtggtgt gtgattagtt
    24001 ctttatagat accacaaaaa atcatagtcg gatcagttac accaagtgtc agcatatatt
    24061 tccgctgatt aatctgccat ttttttaata tagaaattgt tctttcatct agtggaagaa
    24121 ccccagcact acaatcagtt ttaggatcac tgattttata gccatttttg gattggctta
    24181 gtgttttatt gatggtgacc gtattttctt gaaaatcaat gtctgaccaa ttcaatgcaa
    24241 gggcttcact gatcctaacc ccgctataaa agagaaagcg tagtaacgtt ttatcaaagt
    24301 cgtttcgata agtttcaggt ttgctgttta ggtaatcaaa caaggtgatc agttgttctt
    24361 tactgtaaac tttgaccttt tctttatgct tagtggtggt tcttttaggc attaatacgt
    24421 tgtctagtgg attagtctca ataatcccaa gattgatagc gtagctaaaa atacgcttag
    24481 aagttgaatg aagtaccttg tattggtcag tagtttcagc ccagctattt acaatttgtt
    24541 gggctttttt taccgtgact tcattcacac gaagatttcc atattttgga agaatccact
    24601 ttctaaactt gatttcagtt ttcatgaagg tggcttctcg gacagtggtt ttatatgttt
    24661 ctagccacaa gtcataaact tctttaaacg tttcgttgtt cgctttttta ataccgtttt
    24721 tatcgtagtc cacttgcaag cgtgacaagg caagctgggc ttcttttttt gttttgtaac
    24781 ctcgtctacg aaccttatca ggtttgccag tcattttgtt cattccaaga taggcttcat
    24841 agagccaaaa tttacctttt ttagtttcat attgcttgaa tgttgccata atagttttcc
    24901 ctccatagtg tagcaagccg tgggggcggt tggaggaggc agtttattat tattttgaat
    24961 agtgtttatt tagctcagta acaagagctg tgaactcttt tagaagatca ttttcataaa
    25021 attcttgata ctcttttttc tcatctgtat ttttttctga atcattaatt aatgagtcat
    25081 atcgtaaaag cgtatttaga agagctgcga gataagcaat tggggtactc ccgtcaggaa
    25141 attcaattcg agatcctgtt ttaagatatt ttattgcgtt tgttaaataa atcttctgtg
    25201 agacagtcag gttatcttct tcgatgttat tgatagcatt agaaagatta gaatcaatta
    25261 tagagttatt tttttcaata gtttttttca attgcttttc aagcacattt cgcttaattt
    25321 cgggcatcat atgcatatct cttaatgtat aagatccgtt taatagatag gtagtggata
    25381 catctaatac ctttgaaagt tgtttaagcc tggtgttatt gggaagatta tagccatttt
    25441 cccaattact aacggcacct ttactggccg gtggggataa tttacctcca agttgttcca
    25501 ttgagagtcc cttttcaatt ctagccagtc taattctttg tcctgtctcc actgcttcgg
    25561 ggtatttttt tctagccaca ttttcaccac cattcatata ttttctctac tatatcacga
    25621 tttaaatatg tgtgaaacat aaaagttcaa aaaagtatgt taaaattctt gcaaatgaaa
    25681 aggtatgatg gtatattgag tttataagag gtatagaaat gtattcaaga gcctcttgca
    25741 tttttttaaa atctttgtat agaaatgtat gctaacgagg ggggataaat atggaattaa
    25801 aaattgacaa actcacgctt gatgatccag cttttaaaat tattgacgat cgagttgttc
    25861 aacaagtaga gcaaactcaa ggaacgttac tttcattatt agaaaaaaag taccagtttc
    25921 caaaatatat gaacaaggca caagctgcta agtatatgaa cgtttcatac aatactatga
    25981 tgcagaaata tgttcctaac ggtttaaagt tgattatagt tgatggagtc gtaagaattt
    26041 cacaagatga atgtgatcgc tttatggaag aataccaaaa ataaacgcaa gccgtggggg
    26101 caaagggatt aataggagga aataataatg gaatcgacac aaaataaagc aatcgaaaaa
    26161 gtattatcaa caattcttac agaagacaca gcggcagaat tagcaaactt ggaaggcaaa
    26221 gcgctagaag aaacgtttga atgtttgtac gaacaaatgg actatcaaaa attgctacca
    26281 caagaaccaa cggtaagcgg agtattgcgt ggcttgaacg atttagtaca agcagagttt
    26341 aaagaacgac tttctattga agagtatcaa gaaattttgt atcagcaagt tgagttgtta
    26401 gcgagcttgc tagggcttga actggaggac gttgaatgaa aactgaaatt tggaacggac
    26461 acattataag atttgtcgat attaatgatg aatggtgggc agtggcgaaa gatgttgcgg
    26521 aagcgttagg tcttaaacag gttactagag ccattcactc tttgcctaaa gatggggtta
    26581 ctactagtaa ggtcatcgat tcattgggca gaacacaaga tgtaaacatc atcaacgaaa
    26641 aaaaatattt accgcatggc attcaaaagc cgtaaaaaag aagccgaggc atttcaagac
    26701 tggattttcg acatcatcaa agaactacgc cagtccacag gacttgaagg ctttcaagta
    26761 ttccgcatgc tagacaaaga acaccaaaaa gaagcaatga ctaaattaag ccacgctatt
    26821 actgaaccaa aaccagtaga ctacattaaa gcgaatgtga tagccaataa agctgtatca
    26881 actatttatg gtcattccaa aatggtaaaa aagaaagata tgactcctga aatgttggtt
    26941 gatcgtgaac ctattttaga tgagacagta gaattaatga ctgttaaaga aaaatatggg
    27001 ttgcagtttt cagtgagtga gaaaatttat aatcgttctg ctgaattgca gacaacttag
    27061 gagcattaac atgatcgaga acaaagaaaa agccttatcc ataacagtct tggcggacag
    27121 gataaaggca acttaattgg gataaaaccc gatattcttt gttccgatta taccatatta
    27181 aaaaatcgga gggaaccatt atgaaacgaa acttaattat aggaagcatt ttaatcggcg
    27241 gaattttcgg cttattacca ttaattgcaa aaatagctat ctttgcagga ttagcgttat
    27301 atctcttcac tgcttatgac gagtacgact atcaattaag aactggaggg cataagtaga
    27361 tgtacaaaac aaaactatta aaccaactcg atagccttga attagaggaa attaatcaag
    27421 ggatcgctga actagaaaat aatatcggga aaacctactt tggaaattca ttcaatgaaa
    27481 aactaacagt tttgtatgta ctcaaaaaac atgcagaaca caaaataatt tgtcgagaaa
    27541 tcaacgagct aaaaaatcag attttaaccg catggttaaa cattactgat atgcaagaag
    27601 caagggtgaa aacatttaat acatgggtaa agtatcagaa tcaattaaaa ggtgctgaat
    27661 ttgttcggga tgggttgaaa tacgaacttg agcagttgaa gctcatggag gtgtctgaat
    27721 gatttatacc agtattttga aaaaggagga gaggtttatt ggcaaataat tatcaagcag
    27781 caacggaatt acaaaagtcg ggatggtcaa tttatccctt atctcctgga actaaaattc
    27841 caataaaagg ctctagtggt tggaatgatg caactaacga ttctaatcaa attaatgaat
    27901 ggtggacgga agatgcaact agaaatattg ggttaatgct tgaagatcat caaatggtgg
    27961 tagttgacct agatcaacat tctgaaacac gaaacggcgt tgagaattat aaaaaacttg
    28021 cttcaatgta tgatccattt ccttctactt atacggaaat cacaccaggt aaaggaatcc
    28081 atttcttctt taaaattcct gatggtgtaa ttatccaaca agaaacaggt gcatttgctg
    28141 atatcttcgg gcgtgatgat aaaggtaaaa gtttaagcgg gattgatatt atcactaaag
    28201 gagttccaat agcgccaact atgattattt caagaagtgt tgggagagca tatgaaccta
    28261 ttgaaaacgt atctctaaat aatattgcac cagctcccga ttggctagtg aacctactgc
    28321 taaaaaaacc gcaagaaata aagactcaat accgacccgt agcaaaaaca gggacagcaa
    28381 aaaaattaga tatgattgtt caaggcgctg gcgaaggtag tagaaacgat catttaacta
    28441 agttatgcgg ttggttacta tggcacggtg tagataatca aactttagca gaattgattt
    28501 atacagcaaa tgcgtacaat gaaccaccgc taaaagataa agaagtgaat caaattattc
    28561 gatcgatgat taaaaaagat ttgaggggga aagctcatgg ctcataagca taaattagat
    28621 agtattttag actttcccga agccagcgag cgtgaagaca atatcattga attgaaaacc
    28681 tggatgtcac gattaagatg caataaagac gatcagatta aaagtaacag tgttgtgaat
    28741 gcagaattga ttttaaccaa tgatagcaat ttagctggaa caattgctta taacgaattt
    28801 agcggataca ttcacctatt aaaagattct ccttggatca atcgtaccgc tggagaatgg
    28861 gaagacagtt ttgaggatgc cttaacggca tatattgaag aaaactacaa tgtagttttt
    28921 gatgacaaca aaatacacaa agcagtagta aatgtggcta gaaagaatgt tttcaaccca
    28981 gtaaaagaac gtatcgaaaa agttaaatgg gatcaacagc ctagactgga aacactgttt
    29041 attgaccttt taggggtaga cgacaacctt tacacacgag aagtaacaaa acgttggata
    29101 gtcggatcag tcgcacgtat ctataaacca ggaataaagt ttgagatcgt tccagttttg
    29161 gatggaccgc aagggattgg taagagtacc gttccagcat tactttacac ggatgatttt
    29221 ttcaccgatt cattagattc gctaggtgaa aagaaagatg attatatgca gcttcaaggc
    29281 aatgtaatta ttgaacttgg agaattatct tcaatgaaca aaactaaaat tgaacagata
    29341 aaaaacttca tcagtgccaa gatcgataaa attagacctc cttatggtcg gaatgtcata
    29401 gcttgggcaa ggcaatgtgt atttattgga acgtcgaatg atgggcagta tttgaaagat
    29461 gatacaggaa accgccgttt ttatcctttg ccttgtaaga ataagccaaa gatgaatccg
    29521 tttaaaacaa aggatgaata ttttcttcaa gtgttagctg aagcaaaggt gctttttgat
    29581 aaaggacaac gaatctattt tagtcccgat gaagataaag aagtcttaga aattgcaaaa
    29641 gactatcaag aggatgccaa gatggagaat ccaattaaag aagcaatcac taaatatttg
    29701 gaaatggaaa tcccgtataa atgggaagaa gctccagcgt gggcgagacg ttcttattat
    29761 cagaactatc ctgacagtaa atgtaatgag aaaaatttac agcatttcta taatggagct
    29821 actatgcaac aagagtttta tttagttgat agcgtactca ccgctgatat tttggaagct
    29881 gtttttgata aacaagcaaa ggatttattg aatggtcgat cagatgcaga aactaaaaag
    29941 attgccttga ttattagcag tattcctggt tgggaacgga agcaactgcc aaaaagaaat
    30001 aaacgacgag gattttacaa taagtcaaat gcctatgaaa ataaaaatcg agctggaaag
    30061 tacaaaaagt aacgggtagt aacgggtcag taacacctag cccgttacta gtttaccctt
    30121 actgccccaa gggatacagc aatttggtaa cggggtaaca ccttatttcc ttaaaacttt
    30181 tgaaatagtt agctaatatg ctggggaata atgttactgg attttacccg ttacctgtta
    30241 ccaaaacgct agaacccttg tccccgttgg gattgagtgg taacgggtac ctgttacgta
    30301 ggtgttacca cgtgttacga atcaaggagg aaagtaaatg ctcaatatta tttcaacaaa
    30361 caaagcaccg aactttcaat atacagatga aatggatcgg tttctaatga acacgctggc
    30421 atttagtgtg ggattggtaa cagaagatta ttccacattt gatccagaag tattaaagat
    30481 aatggaagaa gaacctgatt ggctgcagga gtcagtagcg tggtgtcaat cgctagtagt
    30541 gggatcattg gtagatagtg gcaactatga tgatacaggt gagctaatgg atgaatttaa
    30601 ttgtctgctt aacctatatg atcgagcgag acaacgagag cttacatcga atgaggataa
    30661 tttgttttta aacatccatg ataagttctt ggctttgcta ctaacagatg atgaattgat
    30721 aactaattta ttggaggtag aataattatg catatggaaa gtattagata cttcgatggt
    30781 gagaaacaca ttgagtattc aaacactcaa gaagatgtag actttattag ttttgaggaa
    30841 gataaaatca ctaaagttaa ttttaaagac ggaacgtgct tgaagatagt ttcgccttat
    30901 attgaataca aatcaaaatg ggaacaagat gattctgacg aacgagatcg ctttggttgg
    30961 taatctatgg cagttaaaaa acaatgtaat catgctggat gtaaaacatt gattgattat
    31021 aggcagaagt attgtgggaa gcataaagca aagcaaacgg caattaagcg tgaagaaaga
    31081 aagcaaagcg aaggtaaata ttttcaattc tatcaaagta gaacatggcg aaaggcttca
    31141 tacttgtatc ggttaaatca tccagtatgt gaggattgcc tagaagaagg cttgataagg
    31201 aaagctgatg ttgtagatca caaaattgaa ctaaaagatg attggaatag acggttagat
    31261 gaaactaatt ttcgttcttt atgccatgca caccataatt ccaagacagc caatgaaaga
    31321 caaagacgag aaaagagtac cctttgagtt aagggggcat agtgtgaaac ttactgacaa
    31381 tcgatgccta ctcattttgg tacaaataac cgttgtgaaa aggcataagg gtaattacaa
    31441 ataacgatta cacttgtaat cacaagtgaa aaagtatata atgaaagtag gagatttatt
    31501 gaaaaataca attgttatta ctgataaaac agggaaacag cgcaccattg agttacctca
    31561 ttttggtcaa gtgaccattc aaatgcagaa cggaaaaatc atttatatag ataaattaga
    31621 taaagaaaaa ttctgatcga aaaacgaagg aatgtgagct taattgctta cgttcctttt
    31681 tcttttgtct gaaaggagga caacatggga caccctaaaa tattacacga tacaaaaggc
    31741 aatatatcaa gtgaagaaaa agcaatccgt gtggacgcta gagaagaatt gttcaagcag
    31801 caaccattaa taaatatcac gccccctgac tggatggcag cgagcgctag aagtgaatgg
    31861 aatcgtatcg taccaacatt aaaaaaagat tatccattga gtgaagcaga ttatggttca
    31921 ttggtagcct attgtttagc ctttgctcga atgaaaacag ccgaagccga gataagaaaa
    31981 tcaggtacgt ttatcacatg cgaaaacgga gtaaagaaag ccaatccagc ggttcgggtt
    32041 caatcacaag ctatgagtga tttgaaaaaa caagccacct cactaggtat gaccttagaa
    32101 tcacgatcaa aactagcttt gaacaaggtt aaaaatgatg aacccgaaga cccgttcaaa
    32161 gagttgatga attcatgaat gattacattg agaaagtcct atcaggtaag ctgattgcac
    32221 ccaagaaaat tatccaagcg tgtgagcgtc atataagcga tttggagcgt tctaagtcag
    32281 atagcttccc ttatgtgttc gatgaagaac aagccaccaa agcgattaaa ttcattgagt
    32341 tgctaccatc tacggacggt aaagcaatca agatgttagg atttcaaaaa ttcattctag
    32401 gcagtcttta tggctggcgt actaaagaag ggaattacag gcgattcaat cgagcgttta
    32461 ccagtatgag ccgtaagaat gggaaaacgt atatcgcaag tggcatggct gccaatgcgt
    32521 tgattatgga acaagaacca gcagaagcaa ggcaagtatt gtttgtaagt aacgctttga
    32581 aacaagctaa attgggctat gatatgctgt ctaattcact tagaaacgtg gtcaagtcta
    32641 gtaagttttt aagaccacaa ctgaaaatta tgaactctaa gattcagcac ttgccctcta
    32701 attcgttcgc tatggcactg gctagtgaaa ccagcacgct agatgggttt gcaccaacaa
    32761 ccgcaattct tgacgagtgg cacgaagcaa aaactcgtaa aacgtacaac gtcattaagt
    32821 caggaatgac ccaacaaaag aacggcttat tgtgtgttat tagtaccgct gggcttgatt
    32881 taaacgttcc tatgtacgag gaatacttat tgttagatcg tgtgctaaaa ggcgaagaac
    32941 aagccgacag gtactttata gcgatatggg aattggacga tcccgaagaa attcacgatc
    33001 aagagaaatg gatcaaagcc aatccgattt ttgaaagtga agaaatcaaa aaagtaatga
    33061 ttccaaccat tcaagatgat gtgaaccttg ctttgaaaca aaataacctt aattctgtat
    33121 tggtgaaaaa cttcaattta tggagacaag cgagtgagga cagctatatg attgctgaag
    33181 actggcaggc aaccgaagta gaaccacaag atattacagg caagcccgtt tatatcggag
    33241 tggatttatc taaaacagat gatttaacta gcgtttcatg gatcgtaccg ctagataacg
    33301 gcgaacttta ttgtgattca catagctttg tagccaccaa atatgggctt caagacaaag
    33361 aaaagcgcga cggtttgcct tatcgagaac tagaaaaagc tggtgagtgt tccattactc
    33421 aattagaaag tggaatcgtg gactatgacc aagtatttca gtttattcaa gatttgattc
    33481 aagaaaatga tttggaatgt atggggattt gttatgaccc gtataacgct aattcgctta
    33541 tcagtaaagc tgaaaaagcc aactacccaa tgttagaagt aagacaagga acgattactc
    33601 taaacgttcc gacccgaact tttagagaac aagtttatga aggcaacgtt attcacaata
    33661 aaaatacgat tctcacccat gcagtgaaca acgctatttt aaaaacggat aacaacggca
    33721 ttcagattaa taaatcaaag aacagtaaca aaattgatcc aatagctgca ttaatcaatg
    33781 cctatgtgtt tgcaatggat tacttcacca caacggaagg agcgaaagca gacaatgaat
    33841 tttatacaag tgaagaattt tctttctaat tacattcata ccgttctttt acttcttgga
    33901 ttggtgtgtg tgttggttgc tatcacctta ctaacaaatg tctactatgg cttgttagcg
    33961 ctgggcatag tgcttattgg ggtagcggtc atgctaaata cagaacagaa gggaggttaa
    34021 aagatggcat tttttaaagc gagacaaaat acaacgggag atcctttcct acaacatgtg
    34081 gtatcaatcc aatcggatga ttacaccacc agctttacaa gcgttcgtgc attaagaaat
    34141 agtgatgtgt ttgcagccgt tcgaattatt gctagtgata ttgcttcaag tccgattcaa
    34201 ttggtcaaaa acaatatgcc acaagctgat aatgaactgg tgaagttact aaacgagaaa
    34261 cctaattcag aaatggatgg ttggcattta aagtttgctt tggcggtcaa tatgttgcta
    34321 aacggtaata gctttgcaga gattaaacgc aacggtgaaa aggtagaaga aattcactta
    34381 ttacctaact caagtgtaac ggttactcaa ttagataatg gtacgttgtc ttatcagatt
    34441 ggtgataaaa aaagacgtgt gaagtctagc gatattttgc acttcaaata tttcacccaa
    34501 gatggtttaa caggattacc accactttat gcgttacgtg atgaactgaa aattcaacaa
    34561 gctggcaacc gcacattgca caatttcttt actcgtggtg tcagtgggtc aggtattttg
    34621 aaggttcata agtcagattt agacggatca gcaaagaacg caattcgtga aaagtttgaa
    34681 gaagccaacg gttcaagtag tggagataat gctcttagaa cgatcattct tgatgaaaca
    34741 atggactaca aaacattaga aataaacacc gatgttttga agcttatcaa ttccaatgat
    34801 tggaacacaa aacaaattgc caaggcgttt ggtgtaccga ttgagcgttt aggcgttgaa
    34861 aatgaacatt ctagcaccgt tcaaagtaat ctccagtata tccaaagtac gctgattcat
    34921 tactttaatg tgtttgtgag tgaattagat acgaaacttg aaaccaatat ccgctttaat
    34981 tccgatcagt tactagagac tgatcctgaa acaaaagtaa aaaatgtatt ggatcaggtc
    35041 aaagggtcac ttctcacgat taatgagggg cggtcgaaaa tggggctacc ccccatggat
    35101 ggtggcgatc gtttactagc aagtttgaac tttacgtatt tagatacgtt ggagaaatat
    35161 caattaaaag aacaggaagg agttacacca gttgaataat gaagaagaaa aggaaaaacg
    35221 gctgacagaa gaagctgagc taacagccga ttctcccaaa gtagagaaag aaaatgaaaa
    35281 acaaccaaca gacggcaaaa ctatttcagg ctatgcgttg aaattcgggc aaccgtcaaa
    35341 agatttaggc ggctttgtag aagtgattac acccgaagca ttaaaagagg tggatttatc
    35401 aaatgtgttc ttattatatg gacacgatta ttcaaagcca ctagctagtg tcaaagcagg
    35461 tacgttgaaa ttgaatgtcg atgatacagg attacatttt gaagcagagc taaccgacac
    35521 gacctattca aatgatgtgt atgagaatat ctcaaaaggg gttgttgatt ctatgtcctt
    35581 tgggttcgtg cttggattag attcattcga caaaaaagaa gatggcacaa ttgaacgatc
    35641 aatagataaa atcaaagcac ttaatgaaat tagtgtcgtg accgttcccg cttatgattc
    35701 atcaaatgtc caagtaaata agcgttctta cgaatcgttt atgagtaaca accaagcaaa
    35761 acaaacaaac aatagcttag aatccacttc taaagcacaa aaggagagta ataacatgga
    35821 aaaaacttta atcgataacg aaaaaacaga attgcgtggg tatgaagaat atatccgttc
    35881 acaaggcgaa gtgcgtgatg gagtcactac tgtaaatgca gcggcagttg ttcccgaaga
    35941 agtaatcggt gaagtctttg atttgaaacg ttcaaattat aacttagctc aatatgcaac
    36001 agtaaaaaca gtatcaaatg gacaaggtaa atatccagta gcaactaacc aacaagcagt
    36061 gttagcaaca aaagctgaac ttgctgaaat tggtgatatt gacgctgaaa tgtttacttc
    36121 agttgattat aaagtagaaa ctcgtgctgg taagattgcc ttatcaaatg aggttgtgga
    36181 agattcagca gtgaatattg tacaagaggt caaagatcag ttagcgaaat tggtagaaaa
    36241 cactgacaat aagcatatca tggatttatt aaaaacattc actaagaaaa cggctgctac
    36301 gttggacgat ttgaaacaac tatacaatgt ggcattagac ccagcattaa ataaaatggt
    36361 aattctaaac caaagcggat attaccacct agatacattg aaagattcag atggacgtta
    36421 cattttacaa cccgatgtga cagcacctag tggtaaatca ttattcggta tgccagtagt
    36481 attgattgca gatacattgt ttgccaatcc taaagctgga acattcccta tgattatggg
    36541 ggatattgca caatctatct ttgttgctcg tagaaatcaa gtaacgactc aatgggaaaa
    36601 attcgattac tactcacaag gacttgcagt gatcgttcgc aacgattata agaaaattga
    36661 tcaaaatgct tcagtgtata ttgagtttac gccagttgta acaccaaaag tatagaaaaa
    36721 tattgggcgg cggtttatcc cactgccttt ttttcattag gagagataaa atggtaattt
    36781 tggatagcat aaaaaaaagt atgcggatcg atcacactat tgatgatgac tttattcaac
    36841 aattgattga tacagcaggc gaatatataa aaagtgctat tgatagtagc gcaacggata
    36901 aagatatgga taattatcag caatttgatt tggcggtgtc attacttact caacattggt
    36961 atttgaaccg tcaagaagcc agcagcgaac ggataccagt aacggtacaa gcattagtac
    37021 aacaaatgag aggtgcttat tatgccaatc attaagaatg taaatgaatt gactgagaga
    37081 gtcaagttta aaaagacaaa acgggtaaaa gatgaagatg ggcagatggt ggatagtgaa
    37141 gaaactgtat ttgaatgttg ggctaatgtg cgttcacaaa tgctaaaaga cgtgcttgct
    37201 agtgtaggta ccattcttga aggaacgttg acgtttatta ttcgatacga tcaagattat
    37261 gaactaacaa acgatatgaa agtagcttgg aaaaacaaga gttataaaat tatttcaatc
    37321 aatgaaggaa cggcgtttaa agattataca acgatcatag ctaaacagat ttcttagaat
    37381 gattacactt gtaaacgttg tgggtattta gtataattaa ggtagtaaat gaagggttag
    37441 ctgcctgaat tttgcaagac ttagctagtc gaaatctatt gttggactga aaattgtagg
    37501 tgtggttgca aacatgctgg actaagaaag atgatagatg atgaacgttc aattttaata
    37561 caggcatgct aggactgatt ggatggtttt ggcaagctga ttgtgtagaa atcaaagaac
    37621 acgtctgttg tggacgtgtt ttttttgtta taattcaaaa gaggtgattt aatggacaaa
    37681 gcagagttta caaattttta ttggaagtat tatctacatt tagaaaatga gtttattaat
    37741 accacagatt ttgtgatgct cgatgagaat aatttcaaaa cattctctat tgaatatcaa
    37801 aaactactac tagcgattgg ttcggagtgt gagattattt tcaaagaact ttgcggattt
    37861 gatagcagta gcaataaaag cataactgat tttaaagctg ttattaaatc tagcgaatta
    37921 atgtgtttgg ataatggtgt tagggttatg aattctttaa cattgacttc actaaaacct
    37981 tttggagatg ggtggcctga agatacacca gattggtgga aaatatataa cagagtaaaa
    38041 catggtcgtt cttcaaatta taaggaagca aatttggaaa atgtgttgta tgctttagca
    38101 agtctatatt tattagagga atatttgcat aagaaagtaa tactagaagg tgaagtagac
    38161 tttataagtc cagagtcaaa tctatttacg ttatcttggg aaagaaaaca ttcgtcgatg
    38221 caaaaagtga cgtttgaaaa agtggattca aattatgtag ctccactcat tgattttaaa
    38281 gaacaagaag aaatttaaaa acccccacgg cttattgaag tttttgtatg ccatattgta
    38341 tgccatttgg tttaaatcgc ttgaaaaata gtgatagata actctaattc atttagtcat
    38401 aaacgctgtt ttgataacct ttatagttct ttgatatggg tttctcattc atggcggaag
    38461 aagaagaata gaacaaaaga atgaattaat aagatataaa aaaagggatt gtgacaaacg
    38521 tcttgtcaca atcccttttt ccaaataaac gatgttcaat cagctgcccc tcggcattcg
    38581 ctcgaaaaac ggcgaaatat gaagaactat tttgccattt gtttctctaa taatcaggtc
    38641 aaaacgttga cttcacagtc tcttccttaa acggtgttct cctgccaact cctcggtcac
    38701 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    38761 agctgaacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
    38821 taataagttc aaccaaacga aaattatgag gagctatttt tgtttggctg ttcttattac
    38881 ttgatgcagg acagcttttt cacaacctct tttcgtttag cactaaattt ccttaaaaag
    38941 tagaaaagct ttcgaagcct cgtttgtttc ttttactgta taataaaaga aaagagtaac
    39001 aatcgctaaa agatataaac aagagaaagg aaatacaaga tgattggtct gatttatttt
    39061 gtggtgatcg ttctggcgaa cacagttggc gctatttccg ggatgggtgg aggcgtgttg
    39121 atcaaaccga ttcttgatct tatcggcgcc cattctgttg cagggatttc tttttactca
    39181 acagttgcgg tattcacgat gtccattgtt tcgactgtgc gacaagtatc aagcgggaaa
    39241 tcactgaatt ggcagattgt cggttgggta tctagtggag ctgttgttgg aggagtagtc
    39301 ggcaacattg tattcgaagt ttttttacag ttatttgaaa acgagaagca tgtgcagctg
    39361 atccaaattt ttttgacggt attgaccttg gtttttgcgt tcttctacac caagcatcat
    39421 cagccaaaat ttcatttgac gtcatggact tggtacctta tatgtggggg ggttctgggc
    39481 tttttagcta gttttttagg tattggtggt ggaccagtca atgtttcttt gttgatgctg
    39541 atgtttgcat tgccaataaa agaagcaaca ctatattctc tatctacgat tttcttttct
    39601 caactggcaa aattggtaac gattgcttta acaagtagct ttatgcgctt tgatttatct
    39661 atgttgtttt atgttatccc cgctgctgtt attggaggtt tgtggggagc aagatttagc
    39721 agggttttgt ctccaaaaaa agtgaccttt atttttcaag taattgtcct cgtggttctt
    39781 ttgatcaatc tttacaatgc tttcgtcatc ttacaaacat taggagaatc tagactaacg
    39841 caagtcaaaa tcactgaaac atattgttct aaatgaaaaa aggagtatac tgtgataaaa
    39901 aaattcattt aaaaaaataa aaattggctg gtgatttgga tgttgcggga aatgcgttta
    39961 ggagatgcag aagcactacg agaaatcaat gcggaacaat taggatatga tgtaccatta
    40021 accgttacgg tgagacaaat gaagaaactg ttgaaggagc cagaaaaaaa ttttttcttg
    40081 gtctatgaag atcctacttc taaaaaagca gctggctatg tacaagcgga gtcctatgaa
    40141 tcgattttca gcgatccgat gttcaatatt atggcacttg ctgtgtctaa acatgctgaa
    40201 aaaagaggaa tcggaaaggc cttgatgact ggcgtagaag aagcgaaaag acgaggaata
    40261 tcagcaataa gacttaattc tgcagaatac agagtagaag cacaccgatt ttatgaacgc
    40321 atcggttatc attccgataa aatgcaaaaa agatttttga aaatcctgta aatttttatc
    40381 aaaaaatacc tggtcatcaa gccgatactt gatgatcagg tatttttttt gactaagaaa
    40441 gtaattgcat caagaaacct gagatgatca aaacgatggc accaccaagc cgattgccca
    40501 tttgagcaaa agcaataagt tccatccgat tagctgcaga taagacagct acatttcctg
    40561 taccacccat cgagttgttg cataagccag cagtaatagt tgattcgatt ggatataaac
    40621 caaacaaacg accgacaaat cctgaaacta ctgaaatcgc gataacactt gtcaaacata
    40681 agacaacaaa ttgccatgtc aaagcagaag ccaatacatt tagattcaat aacgcaatac
    40741 cgataccggc aagaacagct gctgtcaagt ttttgacaat caagttgttg aacatgatag
    40801 ctgcatcttc gtaatattct ggtaataggt ttgcgatttt acagataaca acgataatga
    40861 tcatgaatgc gtaagcatgg actttaggaa caaaatagtt acagatttgt cctaagatga
    40921 agaaagtcaa tgataccatc aaaccgacac ctaattgtgg aatggaaggt tttacatttc
    40981 taggttttcc atcgccaagg tcgccttttt ccattaattt accattaccg ttgaattttg
    41041 gcattgattg tcccgcacga gcaactaacg ctgcaccaat gatagctagg acatttgtca
    41101 ttgctgtcgc aggaatcagg cgggaaatga tagaagaagc gtccgtacct aaaacatttg
    41161 cataaatcgt tgataaaggt acagctccgg ctccaacacc accagccatt gcgggcaatg
    41221 agatatataa aacggaattt gcaaaaccat ttccgataag catacctacg agtcctacgg
    41281 caaaaaagga gacgaccatt gaaataagtg caacgggaat aaaacgaaca gaagctttca
    41341 tcaatagatt acggttcata ccaaggatgc taccagtaat caaggcagca atataaaagt
    41401 ctaaaaagcc catattgctg acaaagtttt ctgtggattt tactacatac tcaggtaaga
    41461 tcccaaatgt agcaatcgcg gcagaggcaa agatcgcaaa aacggagcca ccacctaaat
    41521 aacttttgac gatagggatt tttgttccga taaaatgaaa taaattaccg aaaatgacca
    41581 aaacagcgat tgggccaatc atattcgtcg gtaattttcc taaagccatt gcaatgatca
    41641 ggacaaggac catgatcaaa taaaggggca aacttacgcc ggtaattttt gttgaccaaa
    41701 acccttcttt tttcttttcc tcaactgcag ttgcggttgt tgttttttgt gccattttta
    41761 cagatccttt cttattctgt atcgtatgaa aaaagccaaa gagaagcggc aaaagagcag
    41821 tagttggctg gctgctgttt tgccgcagtc gcttttttct ttttagacgt catacaaagt
    41881 ttctgtatgt tcttttacta taacaatctt aatcttctaa agatttatat tcaggaaccc
    41941 atttagcaga ttcaacagct tctttgatat ccgtgatttc ttcacgattc aatttttgtg
    42001 caacaactga ttttgcgaca gtttcagcaa tcgtttgaga gaattcagtg atttttgcta
    42061 ctggaggcaa gatagctgct cctggttttg ttacatcaac gattccgcct aatgctcgac
    42121 tagcttgaga aatgatttca gcatttactc gtgtagctgt tgaagcaatc aaaccaagcc
    42181 ctaaacctgg gtacatcaac gcattatttg cttgaccgat ttggtaagtt acaccgttat
    42241 attccacatc agcagcaggg atacctgttc caactaaagc ttttccgtcc gtccattcaa
    42301 tcaaatcttt tgcttttgct tctgccaatt ttgttggatt agacaaaggc atgatgattg
    42361 gacgtggggt atgagccgcc atttctttca cgatttcttc agtgaatgct ccaggttggg
    42421 tagaagtacc gatcatgatc gttggatgga ttgcttttac aactgcttct aagtttgtca
    42481 attcatcagc attagtgaat tctgagcgtt tacgagcgaa tgggatttga ccaggagtca
    42541 atccttttgt atcttcaaac aataaaccct gtttatctac ttgatagaaa tgttggcgtg
    42601 cttcttcttc aggtacacct tgacggatca tttcatctaa aaggatgttt gcgataccta
    42661 caccagcagt accagcacca aatgtcaaga tcgtttgatc tttcaattgc tcacctgata
    42721 tattcaatcc acctaataca ccagctagta caacgattcc tgtgccttgg atatcatcat
    42781 tgaaagtagt gattttatct tcgtattttt ctaagatcgt tgcagcattt cctcgtccaa
    42841 aatcttccca atgtaataac aattctggga ataattcagt agctgattga acgaattgat
    42901 caatgaattc gtagtaagtt tcgccttcta cacgagcatg acgattccct aagtataatg
    42961 gattattcaa taattcttcg ttattagtac cagcgtcgat tgatacaggc aagacttgtg
    43021 caggattgat accagccgca gcagtgtaga ccatcaattt accgatagcg atgtcaacac
    43081 cgttcacacc ccagtcaccc atacctaaga ttccttctgc atctgtcaca acgatcaaac
    43141 gaatgtcgcg tccatctgct gcgtttttca aacttgcttt gatattttct ggttcgtcga
    43201 ttgaaagaaa tgcagcatct tgtggtttca agaaaatttc gttgtattgt tcgattgaat
    43261 ccgcaacgac aggatcataa acgacaggca tgaattcaac taaatgttcg cccatcaatt
    43321 tataaaacaa tgtccggttc gtattgaata gattcattaa gaagatacgt ttttccaaat
    43381 cggtctgttt gcttaagtat tgaccgtaag cttggacaga ttgttgttcc aatgtttgga
    43441 cagtacttgg aagcatacca gtgattccgt attttgcccg ttcttctttt gtaaaagctg
    43501 ttcctttatt tagaaaagga ttatttaata agtttagccc tgtaagcatg aatgattcct
    43561 cctaagtttt aatacaaacg aagtatagga gtcagatgag gatatagtca aaaagagcag
    43621 gtataataaa ttttatttat atgagtaata gagtcaaaag aaatcaaaat agcggtattt
    43681 gttcttttta tatcaacaat ccttgcttga ttactatgcg tatctcttat atgctaaaga
    43741 aagaaaagta gaagaataaa ggatatgtta gatagataaa tagaatctct gaagaataaa
    43801 tcacgtgatc gtgacgataa aatacactta cgtcaggtga taaaaaatac ataagtagaa
    43861 aactaagagg aggaacagaa aatgaaagaa gttcaattga tcagaaaaag cgaactatca
    43921 gaaggtggtt gcaatgcatg cggcgtagta gaagcaacaa gctatacatt aaagcttggt
    43981 gccaacaaag cgataatctc tgagttgaca gtaggcggtc ttgtggacag tctggcgctt
    44041 gctgaaggtt ttattggaga agatatttat gagatgtttt cagaaatccg tcagttaaaa
    44101 aaaggggaaa actgcataga ggttcatcat gagagcccga atgtgagatt caaacgtggt
    44161 gataatgaga tgatcttcaa taatcatgtg tccgatcaca ccgagctata tggaatcgtg
    44221 aatcaaatac taactgagct ttttggattg ggaccttatg cgttcaagga agaaaacggt
    44281 aatccaaaat taaacgaaga atggcaagaa acgattgaaa tacaaagaaa caacccgcac
    44341 ttatttcaat aggcatccac agtatttcgt aaaagggact ttgacacgac ttttgtcaaa
    44401 gtcccatcgt tttttatcta tttgtgttct aaacatttct ttcagagtag taacaactgg
    44461 aatgactgaa atgtaaacag attatttgcc tgtatcaata cggaagagta atataaagtg
    44521 tgtaaacgct atcatattat tcattcaatt ttttatagag ggaaggcatt gaaatggaaa
    44581 aaacgtatca agcaggtaca aatgaaggaa tagtggattt tatcaatatg gaagatttag
    44641 aaatagccgc ttcacaagtg attccagctg gtggttatgg ttacatcagc agtggtgctg
    44701 gcgacttatt tacttatcaa gaaaatgaac gggcattcaa tcatcagctg attattcctc
    44761 atgtattaaa agatgtagaa ttacccgata caactacaca ttttgacaaa gaaacgctga
    44821 ctgcaccaat catcatggca ccagttgctg ctcatggctt agctcatgtg aaggcggaga
    44881 aagcttctgc aaaaggtgta gcagattttg ggacgatcta tacagcaagt tcttacgctt
    44941 cttgtacatt agaagagata agagaagcag gcggggagaa agcaccgcaa tggtttcaat
    45001 tttatatgag caaagatgat gggatcaatt tggatattct tgaagtggcc aagcgtaatg
    45061 gagcaaaagc aatcgtattg acagcggatg caacagttgg cggaaaccgt gaaacagacc
    45121 gcagaaatgg gtttaccttc ccactgccaa tgccgatcgt tcaggcttat caatcaggag
    45181 taggccaaac gatggatgct gtgtacaaat cttccaaaca gaagcttagt ccaaaagatg
    45241 tagaatttat tgctgctcat tctgatttgc cagtctatgt gaaaggtgtc cagtcagaag
    45301 aagatgtcta tcgttcatta gaatctggtg ctggcggaat ctgggtatct aatcatggtg
    45361 gccgtcaact agatggtggt ccagcagcgt ttgattcgtt gcaatatgtg gcagaagcag
    45421 tagataaacg tgtaccaatc gtgtttgaca gcggtgtacg acgcggacag catgttttca
    45481 aagcaatcgc atcaggtgca gatcttgttg ctattggcag acccgttatt tatggattgt
    45541 ctttaggagg aagtacagga gtccatcaag tgtttgattt ctttaaaaca gaattggaaa
    45601 tggtcatgca attagctgga acacaaacag tagaagatat caaaaaaata aaattgcgtg
    45661 agaatcgttt tatttaagaa acaaatgacg aaaaaaatag gagtgcggca gatgatgccg
    45721 tactcctatt tttatagttc aaggatgtca ctaatagaat aatttgaaac gaaaaaataa
    45781 aattgaaata ttttttcata cttaataaaa aaggaagaat attcatgtaa acgattttat
    45841 tgtttttttc tcttttatac aaaaagggtc ataatttcaa gtaagcggat gaattcatga
    45901 ggattttttg catactgtct ctactatact taggtcacta agaaaaagga gtgatttatt
    45961 atgaaaaaga acatgattgg gacattatta ctttcaacac ttcttctagg cggcttggca
    46021 acaccagctt ttgctgaggg tcaagcgact tcaaaaggag atattacctt tactgaacca
    46081 acaaataccg tggagcctct taaccctact gatccttcaa aaccagtaga gccagcagat
    46141 ccggaaaatc cagcaactgg ccagacaggt cctttaacac tggatgtagt tcccgaattg
    46201 ccatttggta cacacgagat tgaaagcgga acaaaaacgt atcaagtaga cgcttcaaaa
    46261 aacgatacac cttatctcca agtatcagat cgtcgaggtg taggtgcaga tggccaagca
    46321 caagggtgga acgtgacagt ttcggtttca gattttgtaa acggttcaca agttttgcaa
    46381 ggcgctgaat tagattttgg aacatccact gttaaaagta cgtcagataa cgaatccact
    46441 ggaccaacta gtcaaactgt aactgggttg agtaaagcaa gtgcagcgac accaatattt
    46501 acagcagcaa aagatcaagg tctgggaaca tggctttctg tttatgatcc agcaaatatc
    46561 acactgaaag tgccgaaagc agcagctgga acattcacgg ctgatttgac ttggaacctc
    46621 gtggctggcc cagtagcata gagtatgaaa taaattcaac aaactgcggt gaaaatacta
    46681 taggtgaagg attttttacc gcagtttttg ttgaaaggat gtggtggaca atgaatcaaa
    46741 gtaaacgact gtttgtttcg tttttttcta ttttaagtct atttttcatt tttccgtcaa
    46801 tcagccaagc aaaggattca gcaggagact ttggaatcaa accggtattt ccagagaacc
    46861 aaatagataa agcaatcggt tattttgatt tattagtggc accggaacaa aatcagacac
    46921 tagaagtaat tatcagcaat tcttcagatg aagaacgaac atttgaggtc tctgtcaatc
    46981 cggcggtaac gagtgatggt ggaacgatcg attatagtca aaaaaaccca acgttagatg
    47041 agacattgcc gtttgatgtc cgtgacgttt tgctcattgc gaaaaaggaa atcaatgtat
    47101 cagcacatgc agaaacaact gtacctatag aagtgaaaat cccagcaaaa agctttaagg
    47161 gacgtgtatt agcgggtatt catgtttcac ctaaagaaga agctgaaaca gaaaatgcta
    47221 cagaaggagc acaaatcaaa aatcgtattg cttataatct cgcagtcgtt ttacaagaaa
    47281 gtcaagagac gatagaaccg gatctgaaac ttttatcagg agatttggac gaggtcaacg
    47341 caaagccaac tgttcagctt cgtttccaaa atcctcagcc taggattatc agcaatttga
    47401 tatttacgag caagatcttt tatgaaaatc agctttatat tgaaaatacg tctaatgcat
    47461 ttttagttgc accaaattcg aattttcact tgaatcttga tttagctggt gataaagcaa
    47521 aagccggcga ttaccgcgca gaaatcatag caaaatcagg cgacagtaat gaatggcgat
    47581 ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa agtaaatgaa aattccgttt
    47641 ttgcagtaca agaacaatct ttcccatggc tttatgcagg attggttgct gcagttctag
    47701 tgttatggtt gattttgctg tttttgtggg tccacaagaa aaagaaaaaa ggagaaacaa
    47761 catgatgagg tgatttcgtt gaaaacatcg atcttattgg gagggattat tgcaagtttg
    47821 ctgttcttca gctggcaaga gccagtccaa gcagatacca aaagttcagg tacgattaca
    47881 ttttatgatc taagtccaat tccgcatcca agtggttcag tcaatggcgc atcaaaacag
    47941 cagacacagc taccgattta taaagaatct aatcaacatg gcgtattccc ggagacgaat
    48001 gaacaaaaga tccgttttct ttatttgatc attggtatag aattatggct tcttgcgtgg
    48061 ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg ttgctacaaa ataaattttc
    48121 attttttcgc tccttctttt ttacttcgct ttttgtttta agtatattta tcttttcacc
    48181 ttcggcagaa gctgttggag caacgcctcc tgccatttct tatgatccat acacgtatga
    48241 aaatatcgta cctccaaatg gtggacgtag tgcagtcaat ggcgggaagc ttgtccaact
    48301 aactggggct aaccaattgc ctaaattgac tgctacttat ttgaacgatg gttcaaatga
    48361 tccttggaaa ggtggcacgg tccgttttca ttgggaaagc catgtgaaac taacaaatgt
    48421 acttgatttg gtgaatatca ctgcacaagg atttgcattt gtggcgatca tcaatttgcc
    48481 ggaacatgta tcaagcagtg atgtattaaa agcaattgac tggaacaacg cctacttgta
    48541 tattgccggt gcccccatca aggttactag cggaagttta ttatcagcag gaagccatac
    48601 cttgcgtttt gggatggggt cgtgggatac aaatactcta ttgacagata ttattgctat
    48661 tattacgcga ggcgaattca acgtggacga tatcccgctt caatttgatg ttacagctaa
    48721 tgtcgcgaag atgacagaaa acggagataa atatgatacg agtccaaaca aaattttgac
    48781 caagggtaaa ttacaacctt ctatgtctaa aaaggccgtt ttttctgttg atttttatga
    48841 ttcgaataat attatccaag atcgtatgtt caattcgatt ttagctccag ctgcaggtag
    48901 attttatcct aaattaactg gcggaaactt gaatccagga gcatatgcat tttctgcaag
    48961 tgcagagata aatacgtgga atagttacat ctcaccaagt gattcaacag gaaaatataa
    49021 tacatttgct gataccacag aatacgtgga tggaaataac cagaaccttc caggctctac
    49081 caatagtcgg acattgacga tggggctgac tgaaggacgt tttgatcagt tgccatttag
    49141 tcgctttgat cgcgtagtca actattttac aggaaaaaac gttacacaag gagcaatact
    49201 gactcatact ccagcaagca caagtggatt gaatcagaca gccaatatcg tgtacagtgg
    49261 gaaagatgcg caaggcggat tgctttctcc tgttgcttta aaggtattgg agcagtacaa
    49321 gctagacgga acggttttac caacaaactt aacagccgat agtcagtggg gaaagctcct
    49381 ttcttctgca tcgtaccaag tcacggccaa ttggtcagca actgaactaa aaaagggttc
    49441 gatccattac aggctttatt cgaaaggaac ccaaaaatta gcgacaggat ggacagatca
    49501 acttttccaa acgatccaat caaataacgg gggtgtaaat acagctactt ctacactacc
    49561 tgcattggct tcagggcaat attattttga ttatcgcttg gtggacgatg tacttactaa
    49621 catgtatccg tcttttgcgt acaaatggca gtctgaacaa gctggaatca ccactcttcc
    49681 tcaaataaca gtggctaatt ttccatctgt gagtgcagat agtcagctaa aaaaccttag
    49741 cagacaacct gagacaggag atcccctgtc tgctctatca ggagatacga ttcaagaaaa
    49801 tgtgacattc acgttaacaa aatctgggga tgcgataacc gacaaaaaaa tcacgatctc
    49861 acttcctgaa aatactactt atatcaatgg ttccttaaaa ttaaatggta caactatcgc
    49921 agataccggc attcaacaag gagtttctgt cccagcagat ctattgagca aaataggaga
    49981 taccattcat cttacttaca actatcaatt aaatactgta gatacttctt tacaatccgt
    50041 cagcatactg acaaaggcag ctgtactttc cagcaatatt acattagcag atggtgctaa
    50101 gcttcctaac ccagttgtcc aaacatctgc taaaacaatt cttgtcccca aacaagaatt
    50161 aacattagtt aatgtaccag atgattttac atttggtaac gatctcccca aaccgttgaa
    50221 gacaagttat tatgaatcaa aaggcgattt ttcttttgat gtgcgggata ctcggcttcc
    50281 aagcacgtct ccttggcaac tgacaggtac attgacttct ttatttaaga atgatcaagg
    50341 tcaagaatta agcggaacaa aattatactt caatcattct ggatcaaagc aattgattca
    50401 gcaaggtcaa aacacgttga tttatgagag tgatggaaca gctaaaggag aggttctcgt
    50461 tgactttccc gataccgatg gtttactctt agaggtaaat agcagtacga atgctcaacc
    50521 tggcgcaacg tatcaaggca tggtcacatg ggaactgaca gctgggccaa ccagctaagt
    50581 gattagaatt tgtatggatc tgtgataact gtcacagatc ctctttttca aggatgagaa
    50641 ggaaacttct gctgttaaaa tcagaaatgc ttgaaggagt ggacttggaa cttttcagtt
    50701 tgcttgaaaa aaatgaacaa cggcaaatca aacttttcca acttttatta gaggaaaaag
    50761 gagaggtttc cgttaataac gtacttaccc gcttatcagt tgatcgggct accttgaaag
    50821 aagatctgtt ttatttacaa gctaacttgg aaaatatcaa acaattggaa attgttatcc
    50881 aagaagggaa tattttatta atcagacatg gaaatatagc agcagtcgat gtttattatc
    50941 attgttactt gcgctattct acaaaatatc agatacttct ttatctgttg gaaaatggct
    51001 catttgatcg gcagaaactt gtcatggaac ttaatatcag ttcggctact ttgtctcgca
    51061 gagtgaagga attaaatgat tctttaaatg agtttcatct gaaaataaaa agcgggcggc
    51121 tgaccggtcc agaaagtcaa attcgttatt tttatttcca attattttgg tttggacgtc
    51181 cctatcttga aaacaaaaaa gaatacagtg atttatcagg aaacactttt ttagatctgc
    51241 tgcaaaaaga gtttagtttt gctattacag aagaaggacg attgaaattt gaaatatggc
    51301 tttccattat gaagaagcga atgcgctgta cgaaaaaatc caaaccagat tatagcaatc
    51361 ttcctgttgg attgtttcaa acgacacaat ttttattgga cctccataca tttttgaccc
    51421 gttacttctt tcaatatgcc tttccttgga atcaaaatga aacgttggtt ttttatcttt
    51481 tcatgatcag taactttaca ttagatacac ataactcgca tgtccaggag tttttgataa
    51541 gcgaaaaata caaagacgac gtagtagctc aattgaatcg aacgtttaaa caatcgatag
    51601 aagaatattt ctctttttat gtttttccag aagagatttc gcaaaagatt gcgttgacag
    51661 tgatgcaaaa tcacgctcat tttgcctatt ttagaggatg gatgaatatt tttggccaag
    51721 aaggattaaa acagcggtta actgaagtag aagataaaca attcctggtt ttatgtgaaa
    51781 gattggtcag acaaacattt gctattgttg attttactga agtagacgag gaaaatgccc
    51841 gctttgaatt atttgctcgc tatgcatcta ttttacggac gctgatcaag cagttagaat
    51901 tacgtctgca aattgcttat gatcttcctt atgaaaggct aatggcggat atcgtgaaag
    51961 aaatcatcca ggaacaattg ctgaatacga tcaaatatga tttagtcgaa tacgagaaag
    52021 aaaagcagta tgatatcatt ttgactagcc aattgaaaga gtacccgtca cagaagtccg
    52081 ctaaagtttt tgtattcact agcaacgaat ataattatga tttcccctat ttgaaccaat
    52141 ttttaaaaga atgttattta gaaaaattga atttaaggat gaataaaaca tgaaaatcgt
    52201 gatcattggt gcttcccatg aaggcatcac agctgcccta aatctaagaa aacttcaacc
    52261 gaaagcagaa gttttactga tcgatgaaga tcataaagat ggactaggct atgtttcaaa
    52321 tggaatcaac ctttatttaa aaggaaagat acgtagttta gctgaagttg cccataacat
    52381 gcggactctt caagaaagtg gggcaaaact gatcaccgaa tggagagtaa ctgaacttga
    52441 tccagacaaa catcagctca tcctcgcatc caaagaagga aaaaacgaga ccataaccta
    52501 tgataaatta atcgtagcga caggttcctc tcctgtcacc ttgtataaac aaattgaagc
    52561 tgaaaatgtc tacacctata aaaatctagt gcaatctaag caagtacttg ccgctttaaa
    52621 agaagcaaaa gaagtagtga tcttcggtgc tggttacatt ggattggaat tagcagatgc
    52681 tttgagaaat aaaggataca ttattcatct agttgattat atgccgaatg ttttatcaag
    52741 atatttcgat aaggatatga tcaacagttt tcaaaatcag ctgcaaacta agcaaataaa
    52801 tttttatcca aatgaattct tgatcgattg gaaaaaatca gaagagaaag tagtatccgt
    52861 acaacttctg tctcaaacaa taaaggcaga tatggtcatt ttttctgccc aaactcgtcc
    52921 taatacgaca ttgttaaaag aaaaagtggc tttgtatgag gatgagactg tgatagtcaa
    52981 tgaatatctg cagacaagtg atccggacat ctatgcgatt ggagatatcg tcccagtcag
    53041 ttttgacaaa aataaacggc atctcttttt acctttagtt actcgagctg tacatatggc
    53101 aagagcagta gcgctaactt tgagtggtca gccaaccgct tacgacctca gacaaaagat
    53161 aacagcagca gtcattatag actacttttt aggaactgta gggctgacag aagatgaagc
    53221 gccattcttg gaacaaaata ccggttcgtg ttcaggagaa tttgatctat ttccacaata
    53281 cgacgaagaa aacaaaacag taaatgcaaa gctgatctac catccggata cattagaaat
    53341 aattggcgga cagctgataa gccaagaatt cttattatca gatttgaacc tgcttgcgga
    53401 tatcgtgaaa cacaaaacta cgattccaca gctagcagtt gaggattttg gatttctgtc
    53461 tgaatacacc tctcgttttc actacctcaa tgagttagct tttaaagtac tagctaagag
    53521 agcaaatagc aatagagtag ataaaagact gtgagaaata tcacggtctt ttattgtgtg
    53581 gggatataat aaatattaat caatcgagaa taaaaaattc atataatgaa atagcggaag
    53641 aattttttga gcataatatt cgccaaaaat ttatcaagtg ataaaattgt tgtataaaat
    53701 gattatttca ggaggaggag ttaaatgttt ttagcattaa atgaaattat gcattcaaag
    53761 ttacgttatg cgttagttgc aggggtgatg tttttgatcg cctacctggt attttttttg
    53821 actgggttag cttatggctt agctcaagat aatcgaacag cagtcgacaa gtgggaggca
    53881 gattcgattg tgctttcgaa agatgcaaat tcaaatctag ggatgtccat gattactaag
    53941 aagatagcag aagaagtcga aggtgggaaa gtggcgtatc ttgcccaaac acctggagtc
    54001 gtgactagta aagacagtac agaagaagga aaaatcaatg ttagcttctt cggtatcgat
    54061 aaaaatcaat tcatcatgcc caatcttgtt gaaggaaaag cttttgataa cgatgatgag
    54121 gcagtaggcg atattagttt gaaagaagaa tatggtctag ccataggaga tacagttaag
    54181 ttatctggta gtgacaagac gtttaaacta actggcttta cggatcatgc caaattcaat
    54241 gtctcaccag ttctctatac aaccatcaat gcttaccaag aaatccgctt cgaaaaagaa
    54301 gatacaagtg aaaatgcacg gattaacgca atagttgtca gaggtaaaat cagtaattta
    54361 cctgaagatt tagaacagat caaaatttct aaattcatca atgaattacc tggatataat
    54421 gcgcaagtat tgacttttgg atttatgatc ggatttttga tcgttattgc ggctattgtg
    54481 atcgggattt tcatttatgt actgacgatg caaaagatta atatctttgg tgtaatgaaa
    54541 gcacaaggga tcacaggagg atttattgcg cgttctgtag tggcacaaac attcatttta
    54601 tcatttgttg gtattcttct tggtctatta ggaacagtgg gaacatccct tgttctacct
    54661 gatgcggttc cattccaaag caattggctg ttttttggcg tcattagctt gttgatgtta
    54721 gtagtagctg tgttaggcgc actattttct gttcggacaa tcgtaaaaat cgatccgttg
    54781 aaggcaattg gataagaagg aggaagaaca tgaaagcaat agaatttaaa gaagtagaaa
    54841 aaagtttcca agatggcgac caagtagtta aagcattgaa agaaacaacc atgagtatcg
    54901 ataaagggga atttgttgca atcatcggtc cttctggttc aggtaaaagt acttttttga
    54961 ctttggcagg cggattgcag acgcctagca gtggctctgt atttatcaat gatcaagaat
    55021 tcagtaatca aaaagaaagc gaacgcgtta aactacgttt taaagaaatt ggctttattt
    55081 tgcaagcttc caatctggtt ccttttctga cagtaaaaaa gcagttatta ttagtagata
    55141 agattaaaca tgaacatcgt caaaaagaag caaacgaatt atttgagcag cttggcgtgg
    55201 ataagttgat caacaagtat ccagaagaac tatctggtgg agaacggcaa agggtcgcaa
    55261 tcgctcgtgc attgtacaat gatccgtcca ttattctagc agatgaacca actgctagtt
    55321 tggattcaga aaaggcacga gaagtagtcg atatcttggc aaaagaatca aaagacaaaa
    55381 ataaagcaac gatcatggtt actcacgata cacggctgat tgacaaatgc gatagaatat
    55441 ttatgatgga agatggtgta ttgaaagaaa aagaataacc aaaaagcagg tggaaaaatg
    55501 gtccatcgat acaacaaaga tacacagcaa aaaattttga aagaagcgac tgacttgttt
    55561 atgtccaagg gatatctcgg cacttcaaca agagaaatcg cacaaaaagt tgggatcacc
    55621 cagcccaatc tctatcatta tttcggtgat aaagaaaaac tatatacggc agttttagaa
    55681 aaccacttaa aagatgttgg aaaagctttg agagaaatcg ttcaaacaag cgaaacggga
    55741 tttcaagtaa ccttgacaaa aatggcgcag tttttgattg agacacatct ggtcgatttg
    55801 tttctgatgc ttcacgatct tgaaagcaat ctttcaaagg aaacaagaga tcatcttttc
    55861 tttctatgga agaaaaatta tcgtgaacct tttgaagaaa tcttttcagc aaaccaatct
    55921 gtgctaagaa atgggatttc aaaagaaatc gcagcaaggc attttttctt agtgttagca
    55981 ccttacatca ctcggtcgaa aagacctgtg gaagaaccgc taaatgtggc gcaattgatc
    56041 gatttatatt tgaatggtgt attaggagaa gcgtaaagct gttatatagg gagaaaatag
    56101 ttaggtgaag aagcaagata tgacgaagat cgttatatct tgcttcttta tataggtagt
    56161 ttttcataaa tacaaatttg ttattctctc atgattatca gggtatattt ttatttatta
    56221 tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga aattaattgt ttgtagaatg
    56281 aaggaaacta tttattgggt agatgaatca ccacgaatag tcaaaaggag atttcaggat
    56341 atgccaaaaa tgaaaaaaat cattttagca gtaatgttat tttctacggt tatatctttt
    56401 acagcccctt ttttgcttac ttaagctgat tttaagtttt ggcccttagt ctggatctac
    56461 gaggtgagag tcaagaatga aaaaaataat tggagctttt gtatttgcaa tcattatcat
    56521 tattatctgt tttcaaatag atgtttttgg tcaaaaaaag atagttggac aaacggtcag
    56581 cagtacgaac agtacggtta caaagacaaa ggaatcattt gatgatacag aacaagagac
    56641 attaatcaaa atcggaaaaa atgaccgtac actatataca gatgactcgc taactacccc
    56701 acttgcaact ataaacggcg gagagctggc ggagtttctt tctgaaacaa aaaatgctta
    56761 tcagatcaag acaaatgatg gctatactgg atatttagcc ctcgaagatg gaaccaaagt
    56821 cacaaagaat atacaaacaa aaccggaaga gctatctgat gcagtgattg tattagatcc
    56881 tgggcatggc ggaaatgata ctggggcatt aagcaatgat gaacaaacag aagaaaaaga
    56941 tataacatta agtacagcca taaaagtaaa aaaggcgcta gaggatgctg gagcaacggt
    57001 ttatttgacc catactacag atgaattagt acagcttgga gatatatgcg actacagtga
    57061 agaaaaaaag gcagatgtct ttatcagttt acacgcagac tctacggaat atgcgaatga
    57121 agcaactgga atcacgacat attattacta tggacaagag gaaacattag cacagacaat
    57181 tgctgactcc ttcactgatc tgcctctgga ttcaagagga atctccactg ggaactatca
    57241 agttcttcga gaaaatcttc aaccttctat tttaatcgag atgggttata tgaataatga
    57301 tagtgattta aaggaattag taacagacag ttatcagcag cagattgctg caagtttgac
    57361 acaagcattg accacttatt ttcagtaata aataagccca gccaaacaaa aatagctcct
    57421 catatttttc gtttggccgg gcttattatc gcagatgcaa gcttttaaac accgtttaag
    57481 gaagaggtcg tgagcctgcc gttcaactct gagtcacaag gagcaatttc aaaaaacagc
    57541 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcatgag aacaccgttt
    57601 aagaaaaagg ttgtgaaatc aacgttttgt tctgagtatt agagaaacaa acggaaaaat
    57661 agttcctcat attttgccgt ttgttgagct aataccgagg atcagatgat tgaacaccgt
    57721 ttattcggaa caagggactg tgacatgact tttgtcacag ttccattatt tttttagtag
    57781 cttcaaagct ttttctgtat tggcaaaatg aagtttggca tatttttcaa gcataggcat
    57841 gccgcctttc tccaatagtt ttgccgctac ttggtcagat ttcgttccag ctcctgacgg
    57901 aagggtgatt ccagcttcag ctgatgcttt gtctaactct tccaaaaagc tggcacgact
    57961 gatgatagaa gcagcagcaa cagctaaatg atattgttct cctttagtaa cgaagtacag
    58021 tttttcttgt aattgatttt tttctaaacg cgcatatttt ttaaaatttg cttccggcgt
    58081 aaactgatcg atcaaaatcg cttctggttt ttctggctcg atttttttta gcagcaaatg
    58141 gatcgcttga ttgtgcaaag cgactttcat ccgaacggca ttgtattttg gttggatctc
    58201 attgtatttt tctggagata agacaagcag tttatagggg atggtttttt ttaagatgtc
    58261 agcaagctgg atgatttggc tatcttttaa ctcttttgaa tctctaacac caagtttacg
    58321 taattctgca agctgatcac gtttcacata agcagcacat actgttagcg gaccgaaata
    58381 gctgccattt ccgacctcat cactacctaa aacggataat tgactgatat tttttggcaa
    58441 agaagaggct gtttttgctg gcttggcttg tttatcctgt gcctcccccc atttatcagc
    58501 ttctattgct gcattcgtac cttgaaacat cacttttccg gatgtataag cggtaatggt
    58561 cgtcccgtct ttttttgcag caaagctagt ataaggaatc tgtttattta acaaataaga
    58621 gcgatagtat gctttcattt gactaagttg gtcgtttgtt gctttgataa tgacgttgct
    58681 cataaaattc tcctcgtttt tatacataaa actataccgc aactcacgac ttttcgtttt
    58741 ctaagagatt gcgagacata gcattttttt aaagtttatc atataatagt taagtatgtg
    58801 aaaaatattt gtggagaacg gtgaaagaac caacttcatg gtacactaaa taaaactttc
    58861 atcaaatagg aggaaatgat tcgtatggcg cacgaaaaaa cacgctacaa agcggtgatt
    58921 gccaaccaaa catatactat tatcggacgg gaaaccaagc atcatatgga tatcgtaacg
    58981 aagctgatca atgaacagtt agcagaattg aaacaattat caccacaaat ggataatgaa
    59041 caagcagcaa tcttgatggc tgtcaatgcg ctttcggatc aattgaagaa acaagaaaga
    59101 atattggagc tggaagaaga aacagcagaa ttaaagaaaa aaatgatcaa atttacagag
    59161 cttgaaaatc gtgtgaaacg cattgaagca atcgagaacg aagctcggga agtattgaaa
    59221 gaaaatggtc aagcagatca taccattaaa aatcatgtgg aagcacaaca gattttaaac
    59281 gagaaacgaa aaggacaaat taaacaaaaa tcttctaatt aagatccttt tatcaaaata
    59341 agaaaaattt aaaaaagtta gaagagaaag gaacgtagca tgctgagttt gctgatttta
    59401 tttttattgc taattgcgtt tttttctgga gcacgtcgcg ggtttgcttt gcaaatcata
    59461 ttcgcgattg gatatgtatt gtcatttatt gctgcccagc atttttacaa accattagca
    59521 agtcatttgg aattatacat tccataccca gctgtcaccc caacatcaaa gttagcattt
    59581 tttgatcagg tatttgcctt tcacctagat gaagcttttt atgcaggaac tgcttttctg
    59641 attattttgc taattggctg gctgcttacc cgatttgtgg gggtttttgt ccatggattg
    59701 acgtatgtgc cgatcttgag acaggtagac tggattgcag gaggcatcct gtcactaatc
    59761 atggcttacg ttactatatt tttgattttg cagttacttg cctttgttcc attagatgtg
    59821 gtccaaaatc aattcggcga gcatagtttc gctcgtttta tcgttgagag aacgccattt
    59881 cttaccaaca agatgtatga gctttgggta acgaatattt tgaattaagg aaaatagtgc
    59941 tggaaaatat ttcgtttttt gtacagagta gtttatttaa gaaggctgcg tagcgtatgt
    60001 ccggcacagc tcttttttca tagtgaaaga taaggtgata aaatgaataa ccgaatcctt
    60061 gaaacattgg aatttgaaaa agtcaaacag atggtcagac aatttgtagt gactgcacaa
    60121 ggaaaagaag aactggcaga attggtacca gtgagtgaga aacaaacgat cacaaactgg
    60181 cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc gaggcgggat ccctatacct
    60241 aaaattgaaa atatccgtcc ccatatgaaa cggattgaga tcggagcaga cttgaatgga
    60301 gtggagcttg cacaagtttc cagagtattg tctacgacaa gcgaattgaa tcgtttcatt
    60361 gatgatcttt ctgacagtga aatcgaattt gcacgtttgt atatgtgggc agatcagtta
    60421 gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa tcgatgaaga cggacgcgtg
    60481 actgatgacg cttctcctga gttaaagagc ataagacaga acattcggcg aagtgaacaa
    60541 gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa atgcaaagta tttaagtgat
    60601 gcgatcatca ccatgcgaaa tgaccgctac gttatcccag tcaaacaaga ataccgcggt
    60661 gtatttggcg gtgtagtcca cgatcaaagt gcttcaggtc agactttatt tattgaacca
    60721 aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc aaatcgctga acgaaatgag
    60781 attcaacgta ttttaagtga actttctgct gaacttgttc cccatcgtca agaaatcatc
    60841 cacaatactt atgtgattgg taaaatggat ctgatgaatg caaaagcacg atttggtaaa
    60901 gaagtaaaag ccatcgtgcc gggaatcagt gaagacaatc atgtcgtctt gaaacaagca
    60961 agacatccac tgatcgatca ggaaaaagta gtttctaatg acatcacaat cggaaaagac
    61021 tatcaagcca tcgtcattac aggacctaac acaggtggga aaacgatcac gttgaagact
    61081 ttaggtctgt tacaactgat ggggcaagca ggattaccga ttcctgcagg tgaagaaagt
    61141 caaatcggta tttttgagga agtatttgcg gatataggag acgaacagtc gattgagcaa
    61201 agtctatcga ccttttcttc tcatatgacg aacactgtag atattttatc aaaagtcaat
    61261 gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa cagatccgca agaaggtgct
    61321 gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt ctgcttatgt gatggcaaca
    61381 acgcattatc ctgaattgaa agtttatgga tataatcggg caaatacgat taatgccagc
    61441 atggagtttg atgtggatac attaagtcca acgtatcgtt tgctgattgg cgttcctgga
    61501 cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg atacagaagt tattgatgaa
    61561 gcgaagcagt tgatgaatga tgagagccaa gatttaaacg aaatgatcac agatttagag
    61621 aatcgccgga aaatggcaga gaccgaatat ctggaaatgc gtcattttgt ttctgaggca
    61681 caagaacttc acgatgattt gaaagaagct tacagctatt tctttgaaga acgtgaaaaa
    61741 gaaatggaaa aagcaaagaa aaaagcaaat gaagtcgtgt cagaggcgga agaaaaagca
    61801 gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga ttgggcaagg aaatgtgaaa
    61861 gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc tacatcaaga agaaactttg
    61921 aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa cgttgaaacc gggagacgaa
    61981 gtgttggtta cgacttacgg ccaaagaggg acgcttttac gtaaaaacgg gaatcagtgg
    62041 caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag acgagctgac gcctgttgct
    62101 cctcaaaaag agccgacaca acgagttatc catgctgtac gatctgaatc tagtagtcac
    62161 gtgccgaatc aattggacct tcgaggaaaa cgatacgaag aagcgctaag tgaggtagat
    62221 caatatttgg attctgccat tctagctggt tatcctcaag tgactattgt ccacggaaaa
    62281 ggaacaggcg cacttcgcaa gggaattact gattatctta aaaaccatcg gagcgtcaaa
    62341 agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg cgacaattgt taaatttaag
    62401 taaagcttac taaaggtaag cagatggttc gctatccaag tatagaaatg ctatactagt
    62461 catagatttt aaaaatggag gtctttgata tgtcacaagt aattacagat aagacattta
    62521 atgaagaaac agataaagga cttgtattga ttgatttttg ggcaacttgg tgtggtccat
    62581 gtcgtatgca agcaccgatt ttggatcagt tggaacaaga atatgatgaa gaagaattca
    62641 gaattgctaa aatggatgtg gatgaaaatc cagaaactcc tcaacaattc ggtatcatga
    62701 gcattccaac attaatgttg aaaaaagatg gtcaagtagt agaaaaagct gttggtgttc
    62761 attcaaaaga acaactacgt caaatgatcg atcaatattt ataaaaactt acagcaaaaa
    62821 taagctgagg gtgggactga atccagttcc gtcctcggtt tttttgcggt tgagttaagg
    62881 ttattcaaat taatattttt tgagtataat tacgttgtca ggctggattg tttttttgac
    62941 agacatgcta taataacttt aattgaatat gaaggggagt accagatgaa cgaacgaatc
    63001 aaaaacaaat tagctttact tcctgatcag cccggctgtt acttgatgaa agataaaaat
    63061 ggaacgatca tttatgtcgg caaagccaaa atattaaaaa atcgtgtccg ctcatatttt
    63121 actggtagcc ataatacgaa aacggaacgg cttgttagtg aaatcgaaga ctttgaatac
    63181 atcgttacag aatcgaatat cgaagcattg ctcttagaga tcaatctgat aaagaaaaat
    63241 gatcctaaat acaacattat gttaaaggat gataaaacct atcctttcct taagataacg
    63301 aatgaaaaat atccccgttt ggtgattact cgtaaggtac taaaagacaa agcactgtat
    63361 tttggaccat atcccgatgt cggtgcggcg aatgagacaa agaagatcct cgatcgatta
    63421 tttcctctga gaaaatgcaa acctagtcag acgaaggagc cttgtcttta ctatcatttg
    63481 ggacaatgcc tgtgtcctta ttatttcgac gtagatccga aagtctatac gaaaatcgtt
    63541 gatgaagtga aaaacttttt aaacggagga cacgaaaaaa tcgagtcaga gatccatcag
    63601 aaaatggaaa aagctgctga aaacatggag tttgaaaaag ctgcagaata ccgagaccag
    63661 atccgtgcca ttgagacaat catgactcgc caaaaaatga caaatactga tttactggat
    63721 cgagatgtat ttggttatgc gattgataaa ggatggatgt gtgtccaagt gtttttcgta
    63781 agacaaggaa agctaattga acgagatgtt tcaatgtttc ctttctataa cgaagcagaa
    63841 gaagatttct tgacttacct cggtcagttt tatcaggaaa atgagcattt cattcctaaa
    63901 gaagtcttga ttcctgatac gatcgacaag cccagtgtag aagctttgtt agctacgaaa
    63961 gtgctgcaac caaaacgagg agaaaagaaa aaattagttg aattggcgag taagaatgcc
    64021 tcagttgctt tgaatgaacg gtttgatctg attgccagaa aacaggaacg gacgatcgga
    64081 gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc caatacgtat cgagtctttt
    64141 gataactcta atatcatggg gacgaatcct gtggctgcga tggtcgtatt cattgatgga
    64201 aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa cagtcaacgg accggatgat
    64261 tatgcatcaa tgagagaagt catctatcgc agatattcgc gagtgattcg cgaagggttg
    64321 cctttacctg atttgatctt gatcgatgga ggaaaaggac aagtagatgt ggcaaaagat
    64381 gtcttagaaa atcaattagg aatcgatatt cctattgctg gcatggtaaa aaacgataaa
    64441 cataaaacaa atgagttgct attcggtaca gatttggctg tagtaccgct tgagcgaaat
    64501 tcaccagagt ttttcttact ccagcgaatc caagatgaag tccatcgatt cgcaatcact
    64561 ttccatcggc agctacgcag taaaaacagt tttgcttccc gattagatga aattgaaggg
    64621 ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca aatcgttgaa aaatatcaca
    64681 gcagccacga cagaagaatt acaagaaatc ggactgccga aaaatgtcgc acaaaacgta
    64741 tttgataaat tacatcagga atgaggaaag ggcactttac aaagaaagta aagtgtccta
    64801 tcattttatg tacaacaaaa agagcgttta cgttttgttg gtaaataata aatcgcttac
    64861 gtctggtgtc taaggatata tgtgatttct tgaagaagga gtgaagaaat gttagaagtc
    64921 aaggatcttg ttaaaacttt tgggaactac acagctgtcg atcatgtctc tttccaaatc
    64981 cccgatggaa aaatcatggg gctaattggc caaaacgggg ctgggaagac aacgactttt
    65041 cgtttgatct tggatttttt gacacaagac caaggagaag tgttatggaa tggccatcat
    65101 cttggggaga aagattatga tgtcatcggt tatcttccgg aggaacgggg actttatccg
    65161 aaagtaacga ttcaagacca actgctttat tttgcagaac ttagagggaa atcaagaaaa
    65221 gagattgaac caaaaatcga cttttggatg gaaaaatttc aggtaaaagg gaaaaagacg
    65281 gataaagtaa aatctttgtc taaaggaaat cagcaaaaag tccagctgat cgccacgtta
    65341 atccatgaac ctaaattgat cattttggat gagccattca gcgggttaga tccggtaaat
    65401 gctgaattgc taaaagatgg aatattggaa ttgaaaaaac atggttcatg tgtcattttt
    65461 tctagccaca atatggataa tgtagaaaaa atctgcgatc atttgatcat gttgcgaaac
    65521 ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg aatcatttgg ccgtacgaag
    65581 ttattcttgg aatctggttt aagccaacag gaagtagaag aaatcgaagg cgtgaaaaaa
    65641 gtgatccttc atgaagacaa tacgttagag attaccttag aagatcctga agctgggaaa
    65701 gaaatcttta ctcgtgcgac tcagttcggc tacattccta tgtttaacca gcaaccgcca
    65761 actttggaag agattttcaa attgaaagca ggtgaaatcg atgcgtaagt tttggattat
    65821 taccaaagat gtctatttaa aaaatgttaa gtcgatttct ttcttgatca tgatattagt
    65881 cccttttatc atgctgggta tcatttatgt agcaggaaac tttgctcaac aaaatagtga
    65941 tgtcaataaa atcggcgtat tggctgataa tgagcaaatc agtcagcaaa tgagccaagt
    66001 caaaacagat gattttgagt tcaaagcagt tgattctgaa gaaaaagcac aaaaagagct
    66061 ttcggaagaa aaaatcgatg cgtatttagt agtctcaact gagaatgata agataaatgg
    66121 gatgctttat agcgaaaatt cattaggaca aagtacacaa ctactgatac aacaacaact
    66181 gactggcttg caatcgatgc ttcgagcgag tcaattaggc atttcaccag aagaagtagc
    66241 tagcttgagc caacaagcaa gtttgactcg acaaaaagtc aattttgatt caaaaggaaa
    66301 aatgacagtc ggagaagata actcgacagt ccaatacgtc gtaagttatg tagcaacgat
    66361 tgtcttgttc attattattt tgacgtatgc gcagatcatt gcacaagaga tcgcttctga
    66421 aaaaggcacg cggatcatgg aagtgatcct gtccagtacg actgcacaaa agcattttta
    66481 cggaaaatta acaggtgttt tacttgttgc attaacgcaa atggcattat acggtattat
    66541 ttttggtata ggatacagtc aattcaaaga tatggaaatg gtcaaaaata tattagacgg
    66601 tatctcattg gataacttga ttggaccatt cttgtggtat tctcttctat tcatgatctt
    66661 tggcattttg attttttctg ttttagcagc tttatgtggc tctttggtca acaaagcaga
    66721 agatacagca aaagcaattt tgccagtgac ttatctatct ctaggcggat acatgcttgg
    66781 attgatacta ggagcttcag atccaaacaa tatcgttatt cggatcactt cgtatattcc
    66841 attcttatct tcatatatta tgccgattcg cttagcaaat gagacagtcg gtatgggtgg
    66901 agcattgatt tccttgttgg tacttgtagc cgtaacattc ttattgatga tcttgtctgc
    66961 aaacatgtac aaatcgaatg tactcgttta tagcgaaggc ggattatgga gttcattgaa
    67021 acaatcgatt tcaattatga ggaatgaacg aaaaaaagca taaaacaatc ctaaaataca
    67081 gaaggaagct ggacatctat tgttcccagc ttctttttgt atagacctat tttatttatt
    67141 ttactatatt ggatatatat tcattgaaaa tagtgtataa caactttata atattgagta
    67201 taccaatttt tggaggcgtt ttaatggacc gttatttaga tttactttca gaaaaattcc
    67261 cctcaacgga ggatgtcatc acggaaatca tcaatctcga agcaattttg cagcttccaa
    67321 aaggaacaga gttatttttg agtgatatac atggcgaatt tcctgctttt gatcatattc
    67381 tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga ccttttttct gaacaaatga
    67441 cggaaacgga tatcagccaa ttcactattt ttacggctta tccagaatat gcattaacga
    67501 ctgattggta caaagaacaa gataaatcgc agctgatcca tcatttgatc gatcttttac
    67561 ggtttacgac agtcaaatat acccggtcaa aagttcgaaa aggattgccg aaagaatata
    67621 gctatatcat tgaggaactc ttgtatatcg atgaccgtat caatggaaaa aaagactatg
    67681 taaaaaaaat cattgaacaa cttgttttga tgaaggaaga agaaaaattt ttaacgaaac
    67741 ttgcacagac gatccaaaaa tctgtcatcg atcatttgca tatcgttgga gatatttttg
    67801 accgaggaac tcaagcagcc aaagtgatgg atcgaatcat ggatttttct tctgtagata
    67861 tccaatgggg aaatcatgat attttgtgga ttggtgcgta ttgcggctct gaggcatgtt
    67921 tactgacact tttacggatt gcagcacgct ataattattt atttgaatta gagaaagaat
    67981 atgggttgaa tttacgtcca ttgttttctt ttgctcatca aacttatcag aagaatccag
    68041 tatttgcgcc taaaaacagt aaaaatctat ccgaaagaga gcaagaagaa ttagaaaaaa
    68101 tccatcaggc actttctatc cttcaattca aatcagaaca tcagttattg gatcgtcgac
    68161 ctgaatttaa aatggacgat cgtaaattgc tggaaaaaat cgactatgaa gcatcaacga
    68221 ttgatttaga aggtcagctc tatgagttga aaggaacttg ttttcaaacg atccagccag
    68281 atgatccaaa aaaattcttg gaagaggaac aaaaagtgat tgatagtttg atgtattcat
    68341 tccagcactc tttgagaatg caaaaacata tgacgttttt gatcgaaaaa gggggaatgt
    68401 atctcacgaa caatcagcat gtgctgtttc atggctgtat acctgtagac gagaagggac
    68461 agctcttagc atttgaatta gaccaatctt atcgcggaaa agaactgctg ggcttttttg
    68521 aaaaacagat aactgaaagc gcaaaagatc tgactgcaaa agaagattta gcaaccgatt
    68581 tgatttggta tgcgtggagt gggccatttt cgcctttatt cggcaagagt aaaatggcga
    68641 cttttgaacg ttattttctc acagagcctc atacgcacgt cgagaaaagc aatccttatt
    68701 atcacttgcg agatgaagag tggttcagtg aaaaaatttt agcagaattc gacacaaaag
    68761 aagaagggtc agcaatcatt aatgggcaca cgccagtaaa agtaaaaaaa ggggagtcac
    68821 cgatcaaagc aaatggaaaa gtatttgtca ttgacggagg tttatccaaa gcttatcaaa
    68881 aaacgacagg aattgccggt tattcactat tatgcaattc ttatggtttc caaatcgtga
    68941 cacattatcc gtttttgcct attgaacaac aatttgcaaa aaaatccgac caaacaaatg
    69001 tgaaaaaagt aatcgaacag caactaccac gcaaactcaa tcgtgataca acaaaaggta
    69061 tggaactgca gcagcagatt acccagctca aaaggctgtt ggactatcga aaggacgatt
    69121 gagaagaatt ccagtgaaca ttgttccata gactggagac tgagacaaaa gtcgtgtcgc
    69181 agtctctttt tccggataaa cggtgttctt ctgccaactc ctcggtcaca agtcacacta
    69241 ttcaacaaac atgaaaagct gttttttgaa attgctcctt gtgactcaga gttgaacggc
    69301 aggctcacga cctcttggta taaacggtgt tcaaaagcca gtttctcggt attaagccaa
    69361 atttatgcaa aaatgtgaaa agccattttc acaaaatttt tcttaatact cgaaactaaa
    69421 cagctttttc acaaccccct cagaaagtct attaaaattc attcacaaag aaatcgaata
    69481 agcgctgttc agatggatgt acacggtggg taagttccgg atgccattga acaccgagga
    69541 ttttatgcat atcatctttg ctttgaaccg cttcaacgag tccatcgtta gaaaaagcga
    69601 tggcttttaa taaaggtgaa agatctttga ctgcctgatg atggtaagaa ttcacaaaat
    69661 atggatcggg gagaaggttg cttaacaaac tgtctgaaac aatcttgact tcgtgagtcg
    69721 taaattgcgg ggcagtgggc tgttgttcat gttttactga ccactttgga tataatgaaa
    69781 gatcttgata aagtgtacct tccaaaacga cattcaatag ttggagtccc cggcagactg
    69841 caaaaattgg ttttctttgt ttcaatgctt ccaagatcag tgcctgttca aattggtctc
    69901 gttgaatatt tgtttccgtt agctttggat gaggatcttc catgtaaagt tgtggactga
    69961 catcttgacc acctgcaaga ataagtttgt cgatttgtcc aatcagttga ggagctgaat
    70021 ctggagacat gactggcaga ataagaggca atccttgagc atctaaaact gcatccacga
    70081 atccttgcgg tgtatagctc acttgatttc cttggaaagt atctgttgct cgaatcagtt
    70141 gatttccggc aatgccgatg attggtttca tttgtattcc ccctaaagta gacttattgt
    70201 aacaaggatt taaaaaaaat taaactatgt aagacttgcc cctatactta tctgttcttg
    70261 ctgtgttact atctgtaaag agaaatttcg tgtgaaattc atgacgatag ttgaaaatat
    70321 tgcttaattt gttataataa aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag
    70381 gatctagttc ttcaaggggt gagaaggaca ttttattgaa ggggcctgcc tatactaagt
    70441 tatctttaga ggtacatact ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg
    70501 aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa
    70561 tgaaaagcgt gctcaaacac tgcggcagca gctgaagatc tatgcaacag aaaaagagca
    70621 ggaattggaa acgatccaaa caaactatcg acaacaacta aaacagttgg tggaagaaaa
    70681 agaacaattt gaaatacaag aattagaaaa agaaaaaatg aaattgaaag cagaagcaga
    70741 caaggtgcaa gcaactttga aagatcaata tcaaaagcat cagagtcaag cgatcggtgc
    70801 gatcattgaa agggtgaaag aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca
    70861 tttcagataa aagaaatcag gagaggattt tacaagcagt acaaggaatc caaaacgttg
    70921 aaatccggga tctgtatcaa aatagaacaa acaaccagtg ggtggagaag tattttccag
    70981 ataccacgat tattgataaa gaagcaaaat taagcgcttt aggtaatcgg ttgaaagaca
    71041 tccaagaagc tatccaattc atacaacacc acggcgatag ccgtcaaaag aaactccatt
    71101 tgaaacgtca agaactttcc ttacaagatt tggaagccaa ttattccgaa gaaaatttca
    71161 taaataaatt gaaagaagtc cttgatttaa aagaaaagtg ggaacaacta actgaagaac
    71221 gtgatcgttt agaagaagag gaagaatggc ttcttcattg gcaacagctg gatattgctc
    71281 caagtatgta tgcaagtaag acgaccattt ttgagatggg gactgtgagt gcagcaaatt
    71341 ttgaaagttt caaggaagaa ctgagtaagc ttgatgacgt ttatatggaa gaaatagatt
    71401 atacgccaaa acaagtttat ctttcgtatg ttgctttaaa aaaatcagtt gctgttatag
    71461 aagaaattgc tgggaaatac gggttcaata aagaaacgta tccttatgaa caaaatccta
    71521 aagaaaaact gcaagagacc aagcagcaat tatccattgt tcacgagcag caaaagaaga
    71581 taacgacagc gttaggtcag tgcagtggct atatccaaga atttgaatgg gtagaagaag
    71641 tcacacttgc cgttgcagaa cgagaaaaaa taaaagaacg gtttatccat gcgacttatc
    71701 tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca gaatttgctg catgtgttaa
    71761 acgaaaccgt atcagaagaa gatatatacg tcagtttcga aacaccaaac tcagaagaga
    71821 ttcagcagga agtccctact aaattgaaaa accatccgct tgtcgaacct tttgaattgc
    71881 tgactgaaat gtacagtctg ccaaaatatg aagaagtaga tccaacacct tggatgacac
    71941 cattttatct tgtcttcttc gggatgatgg tcgcagatgt tggttatggt ttgctgatgc
    72001 tcattgggac aatactagct caaaaactac ttgttctacc acgcgggatg aaacgtttcg
    72061 taaaattctt tgagattctc tcgataccat cgatcatctg gggattgatc tacagttcat
    72121 ttttcggaca gtcattacca aaagaactgt ttggcatccg cttgcctttc ccaatcctat
    72181 ctacgacgga tgacgtcaat acgattttga ttttatccgt gattttcggt ctgatacaga
    72241 ttttggttgg attatttgtg gcagcaaagg aaaatatcag gagaaaagat tacttgagtg
    72301 ctgtcagtga tggatttgcc tggcaaggga tcctaattgg gatcgttatt gcgttggttg
    72361 gtgccatgtt gataaaaaat acagcatttg tttaccttgg cgctgggcta tcgatcattg
    72421 ctgccttatg tatcttgatc gttccgatta tccaaacgcc gtctaaagtc aaaggtgctg
    72481 ccaaaggtgc gtataatctt tacggactga ccggctatat cggtgattta gtcagttata
    72541 cacgtttgat ggcattgggt atttcaggcg gaagtattgg agcggcattc aatatgctag
    72601 ttgctttcat gccgccagct gctcgattca gtgtagggat ccttttgatc atcgctttac
    72661 acgcactgaa tatgtttttg acactgctaa gtgcctatgt ccatggcgca cgattgcagt
    72721 atgtcgaatt ttttgggaag ttttatacag gaggcggacg tgcctttgat ccgctgaaaa
    72781 cggcggaaaa atacgtcaac atcaatcaca agaaaaaaaa caataaataa ttggaggatt
    72841 tttatataat gatggattac ttaattagtc aaaatggtgg aatggtattt gcggtattag
    72901 cgatggcaac agcgacgatt ttttcaggga tcggttcagc taaaggtgtt ggaatgacag
    72961 gtgaggcagc agcagcattg acaacaagtc aacccgaaaa attcgggcag gctctgattc
    73021 tgcaattgct tccaggcacg caaggtttgt atggattcgt tattgccttt ttgattttta
    73081 tcaacttagg aaatgacatg tcagttgtac aaggactgaa ctttctaggt gcttctctgc
    73141 cgattgcttt tacaggcttg ttttcaggta ttgcccaagg aaaagtcgca gcggcaggta
    73201 ttcaaatttt agcgaaaaaa cctgaacatg ctacaaaagg aatcattttt gctgcgatgg
    73261 ttgaaacata cgcgatttta ggtttcgtta tttccttcct attagtgttg aatgcctaag
    73321 cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc atctctcaga tgaacgaagc
    73381 agcagagcag gaaagagcgg cgttagaaca agaagagcga atgaagatcg atcaaaactt
    73441 tgaacaaaaa cgtacgcaag ttgaaacaga acatcaaaaa caaaaagaaa aacaaatcga
    73501 attactcgaa aaaaaatacc gtcagctaag gaatcgccag caagtagaag tacgacaaga
    73561 aaatttgaat gcgaagcagg aatttttaag acgattgttt gcagatgcgg taactgaaat
    73621 ggaaaattgg gatgaatctg aacaaatcca gtttatcaaa aatgcattgt attcattacc
    73681 tttgacagga aaagtagcat tcattgctgg agaaaaatca gcagcctatc tttctcagac
    73741 gttgttggat gaatggaaca atgaacttcc ctttatgatg gttttaagtg atgaaactgt
    73801 tgctgatcaa gcgggttttc tgataaacga tcaaggagtt cagtataatt ttcttttcag
    73861 cagccttgta caagatatcc aaggaacgat gagttttgag attgccaatc aattattcga
    73921 ataaggaggg atcagtgtat ggaataccac gaactaaatc ccttgattcg cggaagagag
    73981 cttgaacttt tgtccaatga gacatttgaa cggatgattc aaacaaattc tgtcgaagca
    74041 cttggtgaaa tattgaaatc cacgatttac agtccatata tttatgacgg atatgaatat
    74101 gacttcgaac agaatctttt aaaggagcag agccgtctat ttcgctggtt aaaggaatca
    74161 gcaccggagc ctgaaatcgt ttggatctat acgatgcgtt atactttcca taatctgaag
    74221 gttttgacaa aagctgagat gacaaatcaa aatctggacc atttgtatat cgacgatgga
    74281 ttttattcat tagaaacctt gaaggatgcc attcatacgc aagtttcaaa tgaacttcct
    74341 gaaagcatta tggacagtat acgagaagta cgtgattatt ttgaagagtc taccattctt
    74401 cagggaattg acgtgattta tgatcgacag tttttgacag agcagcgacg gcttgccgat
    74461 aagctaggat atcaagaatt gctgacagaa gtcattgctt ttattgactt gaccaatatc
    74521 acaacgatgg ctcgaggaat attgcagaaa agaacatatg ggttcatgac gacggtactt
    74581 tccagctcgg gaagtatcga aaaagaagta tttttgaact ttgtagataa agatatggaa
    74641 gattacacga attttctatt agagacagat tatgctgatt ttctgcgccc tgtcatccgt
    74701 gagggaaata ttgatctcgt aaagcttgaa cagttgaaag atgattatct aagttcattg
    74761 tatcaagaag cacaaaccca ggctttcgga ccactgccac tactagcctt tttgaacgca
    74821 aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa
    74881 tcagaaacaa tcagaaagcg ggtgagacaa gtctatgacg cataagatcg gtgtagttgg
    74941 cgacaaagat tctgttttgc cattcaaatt gtttggcttt gatgttcgtt atggtacaac
    75001 caagcaaacc gttcgtcagg caattgaaga aatggcaaag aatgagtatg gcgtgattta
    75061 tgtcacagag caatgtgcag cattgagccc agaaacgatc gaacgttaca aggaacaaat
    75121 gatacctgct atcgtattga ttcccaatca tcaaggatca ttagggatag ggctggaaga
    75181 aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc ttatagtatg aaaaggagat
    75241 agaaattttg caaattggaa aaatcataaa agtctccgga cctcttgtaa tggctgagaa
    75301 catgtctgat gccagtatcc aagacatgtg tctagtggga gatctaggag ttatcggaga
    75361 aattatcgag atgcgtggcg atgtggcgtc tattcaagta tatgaagaga cttctggaat
    75421 tggtccaggc gaaccggttc gctctactgg tgaagcttta tctgttgaat taggaccagg
    75481 gatcatttca caaatgtttg acggaatcca acggccatta gataccttta tggaaatcac
    75541 acaaagtaat ttcttagggc gcggagttca attgcccgca ttggaccatg agaaaaaatg
    75601 gtggttcgaa ccaacagtag aagccggaga aacggtgagt gctggagatg ttatcgggat
    75661 cgtagaagaa acaaaagtta taaaacataa aatcatggtg ccaaatggga taaaaggaac
    75721 gatcaaaaca atcgaaaaag gttcattcac tattgatgaa acagtatgtg tcattgaaac
    75781 cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc
    75841 aatcaaagaa aaattaaacc cggatgcacc catgatcact ggacagcgag tgattgatac
    75901 attcttccca gtgacaaaag gaggagcagc agctgtacct ggtccttttg gtgcagggaa
    75961 aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg
    76021 atgtggcgag cgtggaaatg agatgacgga tgttttaaat gaattccctg aattgattga
    76081 ccctaataca ggagaatcgt tgatggaacg tacggtattg attgctaata catcaaacat
    76141 gccagtagca gctcgagaag cttctatcta tacgggaatc acaattgcgg aatatttccg
    76201 tgacatgggt tatgacgttg ctatcatggc agactccact tcacgatggg cagaagcact
    76261 tcgtgagatg agcggacgtt tggaagaaat gcctggtgat gaaggttatc cagcttatct
    76321 tggttcacgg cttgcggaat actatgaacg atctggaaga gtcattgcat taggatcaga
    76381 aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc ccgtctggag gggatatttc
    76441 ggaaccagtc acacaaaata cattgcgggt cgtaaaagta ttctggggtc ttgattccag
    76501 tttagcccaa aaaagacatt ttccttcgat cgattggata caaagctatt ctctttattc
    76561 aacagaagta gggaagtata tggatcatat ccttcaagct gactgggcat ccatggtcac
    76621 cgagggcatg cgtattttgc aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg
    76681 gattgattcc ctttcagata atgaccgtct gacattagaa gtggcaaaat ccattcgtga
    76741 agattatttg cagcaaaatg cttttgatga tgtcgataca ttcacttcaa gagagaaaca
    76801 attcaaaatg ttgaagatga tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt
    76861 aggtgcttat ttaagtgaga tcatggacgg taccgtatcg attcgtgaaa ggatcggacg
    76921 aagcaaatat atcccggaaa cagaattaga aaaactagat caaattattg gtgaaatcaa
    76981 gcaaacgatg caaacgatcg tttcagaagg aggcatgagc aatgattaaa gaatatcgta
    77041 cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa agttgctggt gtcaaatatg
    77101 aagaattaat cgaagtccgt atgcaaaatg gcgagattcg tcgtggacaa gtattggaag
    77161 tccaagaaga taaagcaatg gtccaaatat tcgaaggaac aagtggtatc aatttacgtg
    77221 actcttctgt acgattttta ggccatcctt tagaactagg tgtatcggaa gacatgatcg
    77281 gtcgagtatt cgatggtttg ggacgaccga aagataacgg accagagatc ctgcctgaaa
    77341 agcatttgga tattaacgga gaagtaatca atccagtagc tcgggattat ccagatgagt
    77401 ttatccaaac aggtatctct gcaatcgatc atttgaatac attagttcgt gggcaaaaat
    77461 taccagtatt ttctggctct ggtcttcctc ataaagaatt agctgctcag atcgcgcgac
    77521 aagcaacggt actcgattca tcagatgatt tcgccgtagt ctttgctgct attggaatca
    77581 cttttgaaga agctgaattt ttcatggaag attttcgtca aacgggcgca atcgaccgtt
    77641 ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga acgtatcgct actcctcgta
    77701 tggctttgac tgctgcagaa tatcttgctt atgaaaaggg aatgcatgtt ttggttatca
    77761 tgacagacat gaccaactat gcagaggcat tgcgagagat ttctgcagca cggcgagaag
    77821 ttccaggaag aagaggatat cctgggtatc tttacacgaa cttagctact ttattcgaac
    77881 gagcaggaag aatccgcgga ctaaaaggat cggttactca aattccgatt ctaacaatgc
    77941 cagaagacga taaaactcat ccaatccctg acttgactgg ttatattaca gaaggacaga
    78001 tcatcttgac tagagagctt tacaagagtg ggattcagcc accaattgat gtactcccgt
    78061 ctctttcgcg gttaaaagat aagggaactg gacccggaaa gacaagagaa gaccatgcag
    78121 ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa acaggcaaaa gaattagccg
    78181 tagttttagg tgaatctgct ttatctgatg tagacaagat ctatgcgaag tttgctgagc
    78241 ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa ccgttcgatt actgaaacac
    78301 ttgatctagg gtgggaactg ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag
    78361 acgacatgct ggacaaatat ctgccagaag ggaagtgagt tctgatggca cgattgaatg
    78421 tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca gttgacaact gccacaagag
    78481 ggcataaatt attaaaagat aagcaagatg aactgatgcg tcaattcatc ctgttgattc
    78541 gaaagaacaa ccagctgcgt caagaaatgg aagagtctat gacagaagca atgtctgatt
    78601 ttgtattggc aaatgcttcg atcacggaac ctttcatcga agaattattt gttttgccgg
    78661 cggaaaacgt tgatttgtct atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga
    78721 tgaattttga atatgatgaa cagctgacag acacaccttt ggaatacggg tatttgaatt
    78781 ccagtgccga actggatcgg tcaatcgatc ggtttactac cctgcttcca aaacttttgg
    78841 aactgacaga aatcgaaaaa acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc
    78901 ggcgagtcaa tgcattagaa tatatgacga tccctcaatt agaagaaacg atctattata
    78961 ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa
    79021 atatgggtac cactgactaa gtggtatctt tttttaaaag aggaaaacga tgattaaaca
    79081 aagcaaacaa ataaagaaac gtttgccggc tgttcagctt ctcgcattag gattttttct
    79141 cctgatttta gttggcggcg gcctacttac cctaccattt tttagtaata gtgggcatgg
    79201 gacaaatttt atcgatgcgc ttttcactgc aacttctgct gtctgtgtaa cgggattgac
    79261 aacattgaat acagcagagc attggaacag tgctggtcaa tttttgatca tgcttttgat
    79321 tgagatcggc ggtcttggtt ttatgatggt tccgattcta tgctttgcat tgataaagaa
    79381 gaaagtcagt ctcagcacac gtattatatt aaaagaagcg ctgaatctag aagaagtatc
    79441 aggtgtgatg aagctcatgc tttacatcct aaaattggcc tttgtgatcc aattgattgg
    79501 tacggttgct ttatcttttg tatttgtgcc tgaatttggc tggttgaaag gtatctggta
    79561 cagtttgttc catgcaatct ctagtttttg taatgcaggg tttgatttgt taggagacag
    79621 tttagccaat caccagcaaa atgtttattt gatcatggtg gtatctgctt tgatcatttc
    79681 tggtgggtta ggcttcatcg tatggcgtga tttactggaa tatcatcaga aaaagaagat
    79741 gacgattcat tcaaaaatcg cattaaccgt tacaggtacc cttttattag gcggattcgt
    79801 tgtgttcttt ttcacagagc gcaatggggc aacgctagtg gaagggaata tgtttcaacg
    79861 attggcaaac acctttttta tgagtgtgac acctagaacg gctggatatt attcagttga
    79921 ttacctccat atgagccatg caggtctgat tttaacgatg tttttgatgt acatcggcgg
    79981 aacatctggg tcgacggccg gcggattgaa aacaactacg ttaggtgtgt tgctgatcca
    80041 gatgcgttcg atgttgaaag gacgtacgag agcggaagca tttggccgta ccatccgaca
    80101 aggcgcagtg ttgcgggcac ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc
    80161 catcatgatt ttatcggtga ctgaaacgat acctaaaact tctggcatcg aatatattgc
    80221 ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg atgggattga cgccagactt
    80281 gactgcaata gggaaattga tcattatagc gcttatgtac attggacgag ttggtgtatt
    80341 gactgtggtc ttctctctga tgacaaaagc acaaagaaca caatcaaaat acaaatatcc
    80401 agaagaaagt gtcatgattg gataatttca tcttctgaca gaaacctagt gatttagtaa
    80461 aataagaaaa taaaataatt tttcttgctt cttggatact ttaagtgcaa aatgttcgtt
    80521 tattttttga ggcactaccc gttgttgaac ctcgggtagt gcctcttttt tattgaaagg
    80581 aatcaataag atttgattcc tttatttatc aataggttat aattcatatt tttttatttt
    80641 ttcttttacg atacttagtc cagaagctaa aatttcaagg ttattagagg atagggaaat
    80701 tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat
    80761 accgttagca agaaagtcat tctcaattat cttagggtca atctcaggat caactggaag
    80821 aattcgatag taagggacac gattcgttgg tttttctact ttgaaaagag aatcaaaaag
    80881 ctgattggct ctttttgtaa gacttaattt agtttctaag atcctatttg cttcatttga
    80941 atggaggatt tgagtgatga cttccacatc taatccagaa gttttcacat ttatattaaa
    81001 aattgcttgt tctatcttgc tttggacatg tactggaact acaagataag caactcgcaa
    81061 tccagaacaa ataaattttg tcattccagc taagtagatt gtttggttag gtaataaatt
    81121 ttggaaagga ggaatgatcg tatgttgata gtaagttgtt aaaaatgaat ggatatcatc
    81181 ttcaataacc catagacctt ctttttcaat cacttcggct aattgctctc gtcttttgtg
    81241 agagatttga aaccctatag gattattaca agaaggcata agaaagacac catgaatctt
    81301 cttttttcta cattcctgaa tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat
    81361 tggaacgatc tctaaatgat ataactgagc caattcaatg aaattagcgt atgtatatcg
    81421 gtctgttgct attctatcac ctggagagaa aagtgcagaa agtgtgatag ccaatccatt
    81481 ttgaacaccg gaaacaatag atagagattc taaggacgga atgactcctt gagtttttaa
    81541 ccaatttgct gcagtagtca attgaaaatt tgtgcctttg ggatcttggt aattaagcaa
    81601 accaacaaga tttgggttat tactagttga ttgaataaac gggacaacga atttattaca
    81661 ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt tcggttactt cattggtaga
    81721 gatggtgatg gactcaaaga cattgggaga aataaatgtg ccacttccaa tattcgtata
    81781 cagcaaacct ttctcaattc catatttata agccttgcca acggtagtaa aatttaaatc
    81841 taaataatca gctagctctc tttgggaggg taactgagtg tttttctgta atttattctt
    81901 taaaatatct tgttctaatt gttgaatcaa cgatcgataa taaggacgct tcaatttttg
    81961 tttgtctggt cgccaagtta acgggtaatt gtcataagaa ttaataggca ttttctccac
    82021 tccttaaaat tgtataccat acaattttac cattgattgt attgatttta gaagttaatc
    82081 ttttgtgagg agggaattgt ctatgaaaat tattaatttt gaggaagtag tagcttcttt
    82141 tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa
    82201 aatcagccaa aatcctcgat tggtggaaat tttacctgac ggaaaaggca aaaatatgtt
    82261 tgcgatgatg cctgcttatt tgggggagaa cagatatttt ggcacaaaag tcattaccgc
    82321 ttttccggat aatcatcagt atcatttacc ttctcattta ggagagattt tcttgtttga
    82381 tgctcaaaac ggactaccca aagcattgat aaacgcaaat gcagtcacct ggattcgaac
    82441 agctgctgta tctgcgctgg caacagatta cttagcaaat ccgcatgcag aaacacttgc
    82501 tttaattgga gctggtcaac aagcattgtc gcatttgact gcgatttcta ccattcgtcc
    82561 catcactaca gtgtttgttt acgatatttt agaagaaaga cgaaatactt ttatcgctaa
    82621 agctaaaaaa cagtatccta aaataacgtt tataaatgca atatcagtag aagatgcagt
    82681 acgtccagcg gaaatcattt gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga
    82741 gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca tttactcctg atacaagaga
    82801 gataagtaca tcgttgatgg aaacttctca aatctatgta gatgattact cagctgcctt
    82861 aacagaaagc ggggacctgc ttatagccat ggcagaagga gatttagagg aatcttgtat
    82921 tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca ttttataaag gagaatcgag
    82981 gccgacgatt ttcgacgcag ttggtctagc tattgaagat ctttgttgtg cagaatatat
    83041 ttataacaaa ttagagaaag gggctttaaa atgaacttag attattacgt agttgatgca
    83101 tttgcagatg aggtgttcaa aggaaatcca gcagcggttt atgtgctaga agaatggctg
    83161 ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt tatctgaaac agcatttaca
    83221 gtgaagaaaa accaagaatt tgagcttaga tggtttacgc cagatcgaga aatcgatctt
    83281 tgtggtcacg cgacattagc aacagcattt gttttattta actattacaa aatacctgat
    83341 gaaacgatta aattttctac tcaaagcggt aatctatttg tgacaagaat taaagattat
    83401 tattacatgg attttcctag tattatgccg aaaaaagtgc cgatattgtc tgaatatgaa
    83461 gaagcgattg gggctaagat taaagaagcc tacttagcac gtgatttatt tttcgtatta
    83521 gaagatgaaa aaacggtggc taaattgcag ccagatttta cagctatcaa aaattttgaa
    83581 ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa aagactttgt ttctagaacc
    83641 ttctttccaa aattaacaat caatgaagat ccagtatgtg gatcagcaca ttctaatctt
    83701 attccttatt gggcagaaag aatgaataaa acaaaattgt cagcttatca aatgtcatca
    83761 aggggcgggg atctagaatg tgaattaaaa gaagatcgtg tgataatagg aggaaaagca
    83821 gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt caaatgaatt aaaaataaat
    83881 tggtggagag tggcaacctt atagctacaa tggttgttaa aaaaagacgc aaactttttc
    83941 aatttaatga aaagtttgcg tctcatttta attattggtg tttgtttttt attcaatcaa
    84001 gagtaaaatg ccccatacta actatcctcg gatgacttcg attttataac catcagggtc
    84061 aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct tttaagtcag taatctcaag
    84121 accagcagct ttatgttgct cgtgtaattt ttctagatca tcagctgcta tagcgatatg
    84181 accatagccg tctcctagat catacgcacc atgatcatag ttgtaagtca actctaattc
    84241 atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat ttgttttctg gaaaatcacg
    84301 gcggcggctt tcttcaaaac caaatgcctt ttgataaaat tcgattgatg cttcaagatc
    84361 tttcactcga acacatgtgt gagccatttt catagattca tctctccttt tccttttact
    84421 caatcataac aaaaagaagt aaggaaatag ttgatttgtg cttgttcatg agaaatttct
    84481 catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa ctattcatgg ataaaccaat
    84541 ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt tatgcagcat atattgtcat
    84601 agaaagagat cagcagattg cagtaatcga ggctccaaat ggtgcttttt ttcttcctgg
    84661 cggggaaatt gaaggagaag agacgaaaga agaagcgatt gtccgtgagc tgatagaaga
    84721 aatgggaatt gtggcagaaa tttcttctta tctgggagaa gctgatgagt atttttactc
    84781 gaattatcgt gcaacttatt attataatcc aggatatttc tttgttgctg attcttggaa
    84841 gcgtatagga gaaccaactg aaaagaccaa taaaatttgg tgggtgagcc ctcaagaagc
    84901 gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc gagagatgga tcgaaaaaaa
    84961 taaaaactga aagaaagcga gtagatcagc tttctctcag tttttttatt tattacagta
    85021 catatttttt gattgcttca gctacaccgt gctcgtcgtt tgatgaagtg atgacatttg
    85081 ctgcttcttt gacaagagaa acggcatttc ccattgctac tcctagacca gcatattcaa
    85141 tcatggataa gtcgttctcg ttatctccga tagccatgat ttcatcttga tcaataccaa
    85201 ggtgctgtgc gagattcgca actgccgcac ctttacttgc ttctttattt agtacttcga
    85261 agtaataggg cgcacttttg accgttgtgt atttatcacg gaaagtttgc ggtaaacgcg
    85321 caattgctgc atctaaaatt tctggttcgt cgatcatcat cattttgacg atgttcatgt
    85381 ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat cttgactaaa cttgcttcgt
    85441 ggatcgtata agcgctgata tctcgattag gcgtgtagat cgctttgtca tcgatcgaat
    85501 ggagatgact gccgacacgg cgtgccatta cttcgatctc taggaaatct tcatgtgtca
    85561 gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg tacgagtgcc ccgttataag
    85621 taataacgta atcattatct tgaaaaaggt tcaactcgtt taactgttcc tggacacctg
    85681 gtaagggacg gccagtgcat aaaacgatcg aaacaccttt tgcaacagca gcggcaatcg
    85741 cttcttttac ttcttttgtt acttctcttt tctcatttaa taaagtacca tcgatgtcga
    85801 tagcgactag tttgatagac ataatttcct ccaatatatt ttaatttatt tcaatcaatg
    85861 cgccattacg gatatggctg gcaaattctt gataagtttc gtcaaataaa tcataatgat
    85921 cacgcaagga cggatcaacc atttcttttg gaaagaaaaa tcgctcatct ccttgttctt
    85981 gtcccgctaa tgcagcaaca agctgactaa cctgtgataa ttctatcaaa gttccgtcat
    86041 ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc tgggcgataa aaatcataag
    86101 gtagatcgta gctagaattc acagcagtgt aataaacagg attatatccg accttttcta
    86161 ctaacaaagt gagttcttgg actaattttg aatcccggtt gccagaaaaa gtagcagatt
    86221 tcagtggttt acgattcaaa aaacgtttag ctaaatcatt taaaatagga tcagcagatt
    86281 cagaccattg agtaaagtaa gtgcccaaga ccccatcatc aagtttgagg tattcttcta
    86341 aagtaaagct tccgtcaaga aatggcagaa gtaattgaga atggagttca aaactgctgg
    86401 catcttcttg atacaactcg tgtgcgcgat gaagcagatg ctccaaaatt acttccatac
    86461 cacgggaaac aggatggaag tacacttgca catacatttg atatcggcta acgatataat
    86521 cttccactgc atgcatgcca ctcatggaaa aagcgatccc gccttcataa ggacgaatca
    86581 cacgtaatat tcgggtaaga tcaaatgttc catattctgt tccagtgaaa taagcatccc
    86641 gaagcaaata gtccattcga tctgcatcga tctgactaga aatcatttgg acaacttgtg
    86701 ggttggggta agttttttgg atcacactag ctaccttttc aggaaaacct tcttctactc
    86761 ggttaagaat ctgatacact tctgtttctg gagaagtgat gatctctaca gtgatggctt
    86821 catgattcgt atggaagata tgttcaaatg tatgagagta aggaccatga ccgacatcat
    86881 gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc atcccagcct ccgttgccta
    86941 ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat tcgtcttgaa atctcataaa
    87001 cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg aaaagtgaaa gaagaagtgc
    87061 ctaattgttt aatgcgtcgt agacgttgga cttctttcga attgatcaaa tctaagatta
    87121 cttgatgctg gacatggata tagttatgga ctggatcacg aaaaactttt tccataggca
    87181 gcatttgata tttataaggg gttgtcataa tgtagctcct ttatgttatt acgttgttcc
    87241 attctctgga tttggttgtt ccattgctcc agctggattt ctttttttag ccagtcggta
    87301 gaatcctgta aatcacttgc agaatacagc ttttcaaaag cttttgcaaa tgcttctttg
    87361 acttctttta caaaaagggg ttggtcaagc agttcttcaa gtgttgccat tgtagcagga
    87421 tccactgcag gatagccatt tgtaccaaag tcttctttta aagcagattg ataaaatcgc
    87481 ttcatgactt gcccacgttt ttcctgatta ccgttgacac ttaagtacat cataacagaa
    87541 atcccatttt tgatccgacg ctgagcgata ccggcaaatt ttttgccatg gatgcttaaa
    87601 tcataagttc caggacaata agaatgggtg atttcgaatg cttgtatcgg tgcctttgga
    87661 aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt catctatggt tatttttttt
    87721 gtgtcacttt gggggaaaat caaagaaata ttcaaaattc cttcatctga tacgatccct
    87781 aatccacctg aatttctgat cactggagcg taacctgcat cttccagtgt ttggatcccg
    87841 tcacttagaa aaggagttcg aacatccttc atacctaaga tgactgtgca gtcatattgc
    87901 cagaaatgta aaaagatttg ctggtgttct ttagaatatt ccgttaatat atctgttaat
    87961 gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat ctagtcgaat cgcagggttc
    88021 attcaaccgg ctcctttgta taaattcata ttttctacaa tattatactc taaaatcaaa
    88081 gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa gttggttgac aaaacaaaag
    88141 aaactaggaa caatgtagct atgagaagta aaggagaatg aataatggaa ttatcacaag
    88201 gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca gcaaggggaa gtgcaagatt
    88261 tttattttga tttgaaaggg caaatggtga caattggcga tacgctgtac atacgttata
    88321 aagagattca ggaggagacg ggcgatgaga ttcctgtaac gatcaaatta gttccagatg
    88381 gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg tttgaaattt ggctataaag
    88441 aacggttaga aacatcctat cgaacaccat acggtatgat ccagattgct acttttacga
    88501 aagaactgca tgttagtttg aaagaccgtc ccacagcagg gaaggttcga atcgattatg
    88561 atctgttcat gggaccggaa aaaatcggcg aatattattt gaccttggat tttacagcgt
    88621 aaatataaaa gaaaagtttg attttttgtg tctgattttg tatgataagg agtagactgt
    88681 gaaaggacgt gtcctgattg gaaattagtg tatttgaagg tgcaaacaaa agcgaattat
    88741 caatgatcga agtagcacac gcgatcttag aacaacgtgg cgatgtcatg gatttttctg
    88801 atttagctaa ccagatccaa aactatcttg ggaaatcaga cagtgaaatt cgtgattcac
    88861 tcgcacaatt ttatactgac ttgaatattg acggcagctt tatctcatta ggggataatc
    88921 gttggggctt gcgttcatgg tatgctatcg attcgatcga tgaagaagta aaccatggaa
    88981 tggacgaaga ggatgaagat actccacgtc gccgtaaacg caagaaagtc aacgccttta
    89041 tcaatgatga tgaagacgcg atcgattaca atgacgatga cccagaagat acagatctga
    89101 cagaagaaga tgacgatgat ctgtttgatg acgatgatga tgaagatgaa gaaatcgctg
    89161 cttataattc tgacttgcaa gaaatcggag cagatgaccc gacagatgat gaagaagact
    89221 tgccaggaaa tatcgaagaa gatttaagta tcatagacga agacgatgat gatgaagatt
    89281 atgaagagga agaattttct gactctgaaa aagaataaac attattttct tacgagaggt
    89341 tgtgacagag acgatcagcc tatcagaata aggaggtgtg acacgatttt tgt
//
LOCUS       DAYYFD010000002        75225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000002 DAYYFD010000000
VERSION     DAYYFD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.002"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..1582)
                     /locus_tag="ACHA25_000091"
     CDS             complement(8..1582)
                     /locus_tag="ACHA25_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8761430.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVIPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(1837..2346)
                     /gene="mreD"
                     /locus_tag="ACHA25_000092"
     CDS             complement(1837..2346)
                     /gene="mreD"
                     /locus_tag="ACHA25_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8761431.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(2350..3204)
                     /gene="mreC"
                     /locus_tag="ACHA25_000093"
     CDS             complement(2350..3204)
                     /gene="mreC"
                     /locus_tag="ACHA25_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8761432.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(3359..3997)
                     /locus_tag="ACHA25_000094"
     CDS             complement(3359..3997)
                     /locus_tag="ACHA25_000094"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8761433.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3998..4648)
                     /gene="rpe"
                     /locus_tag="ACHA25_000095"
     CDS             complement(3998..4648)
                     /gene="rpe"
                     /locus_tag="ACHA25_000095"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8761434.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4661..5560)
                     /gene="rsgA"
                     /locus_tag="ACHA25_000096"
     CDS             complement(4661..5560)
                     /gene="rsgA"
                     /locus_tag="ACHA25_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8761435.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5725..7794)
                     /gene="pknB"
                     /locus_tag="ACHA25_000097"
     CDS             complement(5725..7794)
                     /gene="pknB"
                     /locus_tag="ACHA25_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8761436.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7791..8531)
                     /locus_tag="ACHA25_000098"
     CDS             complement(7791..8531)
                     /locus_tag="ACHA25_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8761437.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(8544..9902)
                     /gene="rsmB"
                     /locus_tag="ACHA25_000099"
     CDS             complement(8544..9902)
                     /gene="rsmB"
                     /locus_tag="ACHA25_000099"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8761438.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9902..10840)
                     /gene="fmt"
                     /locus_tag="ACHA25_000100"
     CDS             complement(9902..10840)
                     /gene="fmt"
                     /locus_tag="ACHA25_000100"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8761439.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10833..11324)
                     /gene="def"
                     /locus_tag="ACHA25_000101"
     CDS             complement(10833..11324)
                     /gene="def"
                     /locus_tag="ACHA25_000101"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8761440.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(11339..13747)
                     /gene="priA"
                     /locus_tag="ACHA25_000102"
     CDS             complement(11339..13747)
                     /gene="priA"
                     /locus_tag="ACHA25_000102"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8761441.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(14000..14305)
                     /gene="rpoZ"
                     /locus_tag="ACHA25_000103"
     CDS             complement(14000..14305)
                     /gene="rpoZ"
                     /locus_tag="ACHA25_000103"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8761442.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(14310..14924)
                     /gene="gmk"
                     /locus_tag="ACHA25_000104"
     CDS             complement(14310..14924)
                     /gene="gmk"
                     /locus_tag="ACHA25_000104"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8761443.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(15411..15695)
                     /locus_tag="ACHA25_000105"
     CDS             complement(15411..15695)
                     /locus_tag="ACHA25_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761444.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15713..16603)
                     /locus_tag="ACHA25_000106"
     CDS             complement(15713..16603)
                     /locus_tag="ACHA25_000106"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8761445.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16623..16841)
                     /locus_tag="ACHA25_000107"
     CDS             complement(16623..16841)
                     /locus_tag="ACHA25_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8761446.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16811..18511)
                     /locus_tag="ACHA25_000108"
     CDS             complement(16811..18511)
                     /locus_tag="ACHA25_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8761447.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18778..19551
                     /locus_tag="ACHA25_000109"
     CDS             18778..19551
                     /locus_tag="ACHA25_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761448.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19632..20201)
                     /gene="thiT"
                     /locus_tag="ACHA25_000110"
     CDS             complement(19632..20201)
                     /gene="thiT"
                     /locus_tag="ACHA25_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8761449.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(20267..20369)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20375..21346)
                     /locus_tag="ACHA25_000111"
     CDS             complement(20375..21346)
                     /locus_tag="ACHA25_000111"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8761450.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(21440..22813)
                     /gene="glmU"
                     /locus_tag="ACHA25_000112"
     CDS             complement(21440..22813)
                     /gene="glmU"
                     /locus_tag="ACHA25_000112"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8761451.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(23316..24134)
                     /gene="purR"
                     /locus_tag="ACHA25_000113"
     CDS             complement(23316..24134)
                     /gene="purR"
                     /locus_tag="ACHA25_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8761452.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(24301..25125)
                     /locus_tag="ACHA25_000114"
     CDS             complement(24301..25125)
                     /locus_tag="ACHA25_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8761453.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(25098..25778)
                     /locus_tag="ACHA25_000115"
     CDS             complement(25098..25778)
                     /locus_tag="ACHA25_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8761454.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25801..26739)
                     /locus_tag="ACHA25_000116"
     CDS             complement(25801..26739)
                     /locus_tag="ACHA25_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8761455.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26984..27835)
                     /gene="ispE"
                     /locus_tag="ACHA25_000117"
     CDS             complement(26984..27835)
                     /gene="ispE"
                     /locus_tag="ACHA25_000117"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8761456.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27989..30562)
                     /gene="mprF"
                     /locus_tag="ACHA25_000118"
     CDS             complement(27989..30562)
                     /gene="mprF"
                     /locus_tag="ACHA25_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8761457.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30703..31509
                     /gene="proC"
                     /locus_tag="ACHA25_000119"
     CDS             30703..31509
                     /gene="proC"
                     /locus_tag="ACHA25_000119"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8761458.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(31588..33144)
                     /gene="rny"
                     /locus_tag="ACHA25_000120"
     CDS             complement(31588..33144)
                     /gene="rny"
                     /locus_tag="ACHA25_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8761459.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(33555..34604)
                     /gene="recA"
                     /locus_tag="ACHA25_000121"
     CDS             complement(33555..34604)
                     /gene="recA"
                     /locus_tag="ACHA25_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8761460.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34713..35954)
                     /locus_tag="ACHA25_000122"
     CDS             complement(34713..35954)
                     /locus_tag="ACHA25_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8761461.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(36194..36772)
                     /gene="pgsA"
                     /locus_tag="ACHA25_000123"
     CDS             complement(36194..36772)
                     /gene="pgsA"
                     /locus_tag="ACHA25_000123"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8761462.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36981..37904)
                     /locus_tag="ACHA25_000124"
     CDS             complement(36981..37904)
                     /locus_tag="ACHA25_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8761463.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38643..39929)
                     /locus_tag="ACHA25_000125"
     CDS             complement(38643..39929)
                     /locus_tag="ACHA25_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8761464.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39922..41190)
                     /locus_tag="ACHA25_000126"
     CDS             complement(39922..41190)
                     /locus_tag="ACHA25_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8761465.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            41442..41903
                     /gene="mscL"
                     /locus_tag="ACHA25_000127"
     CDS             41442..41903
                     /gene="mscL"
                     /locus_tag="ACHA25_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8761466.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41962..42408)
                     /locus_tag="ACHA25_000128"
     CDS             complement(41962..42408)
                     /locus_tag="ACHA25_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8761467.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(42501..42746)
                     /locus_tag="ACHA25_000129"
     CDS             complement(42501..42746)
                     /locus_tag="ACHA25_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8761468.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42861..43439)
                     /locus_tag="ACHA25_000130"
     CDS             complement(42861..43439)
                     /locus_tag="ACHA25_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8761469.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(43516..44046)
                     /locus_tag="ACHA25_000131"
     CDS             complement(43516..44046)
                     /locus_tag="ACHA25_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8761470.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(44050..44910)
                     /gene="rlmB"
                     /locus_tag="ACHA25_000132"
     CDS             complement(44050..44910)
                     /gene="rlmB"
                     /locus_tag="ACHA25_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8761471.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44903..45298)
                     /locus_tag="ACHA25_000133"
     CDS             complement(44903..45298)
                     /locus_tag="ACHA25_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8761472.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(45295..46707)
                     /gene="cysS"
                     /locus_tag="ACHA25_000134"
     CDS             complement(45295..46707)
                     /gene="cysS"
                     /locus_tag="ACHA25_000134"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8761473.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46704..47240)
                     /gene="epsC"
                     /locus_tag="ACHA25_000135"
     CDS             complement(46704..47240)
                     /gene="epsC"
                     /locus_tag="ACHA25_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8761474.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(47326..47565)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(47562..49022)
                     /gene="gltX"
                     /locus_tag="ACHA25_000136"
     CDS             complement(47562..49022)
                     /gene="gltX"
                     /locus_tag="ACHA25_000136"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8761475.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(49192..50349)
                     /locus_tag="ACHA25_000137"
     CDS             complement(49192..50349)
                     /locus_tag="ACHA25_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8761476.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(50383..51753)
                     /gene="radA"
                     /locus_tag="ACHA25_000138"
     CDS             complement(50383..51753)
                     /gene="radA"
                     /locus_tag="ACHA25_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8761477.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51816..52343)
                     /locus_tag="ACHA25_000139"
     CDS             complement(51816..52343)
                     /locus_tag="ACHA25_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8761478.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            52573..53379
                     /gene="proB"
                     /locus_tag="ACHA25_000140"
     CDS             52573..53379
                     /gene="proB"
                     /locus_tag="ACHA25_000140"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8761479.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            53376..54623
                     /locus_tag="ACHA25_000141"
     CDS             53376..54623
                     /locus_tag="ACHA25_000141"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8761480.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54699..55043
                     /locus_tag="ACHA25_000142"
     CDS             54699..55043
                     /locus_tag="ACHA25_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761481.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(55160..55486)
                     /locus_tag="ACHA25_000143"
     CDS             complement(55160..55486)
                     /locus_tag="ACHA25_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761482.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(56101..57303)
                     /locus_tag="ACHA25_000144"
     CDS             complement(56101..57303)
                     /locus_tag="ACHA25_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8761483.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            57420..57935
                     /locus_tag="ACHA25_000145"
     CDS             57420..57935
                     /locus_tag="ACHA25_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8761484.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(58134..58580)
                     /locus_tag="ACHA25_000146"
     CDS             complement(58134..58580)
                     /locus_tag="ACHA25_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8761485.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58627..60105)
                     /gene="msr(C)"
                     /locus_tag="ACHA25_000147"
     CDS             complement(58627..60105)
                     /gene="msr(C)"
                     /locus_tag="ACHA25_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8761486.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(60459..61088)
                     /locus_tag="ACHA25_000148"
     CDS             complement(60459..61088)
                     /locus_tag="ACHA25_000148"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8761487.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(61072..61536)
                     /locus_tag="ACHA25_000149"
     CDS             complement(61072..61536)
                     /locus_tag="ACHA25_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8761488.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(62023..63291)
                     /locus_tag="ACHA25_000150"
     CDS             complement(62023..63291)
                     /locus_tag="ACHA25_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8761489.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(63522..64259)
                     /locus_tag="ACHA25_000151"
     CDS             complement(63522..64259)
                     /locus_tag="ACHA25_000151"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8761490.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(64326..64895)
                     /locus_tag="ACHA25_000152"
     CDS             complement(64326..64895)
                     /locus_tag="ACHA25_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8761491.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            65023..65895
                     /locus_tag="ACHA25_000153"
     CDS             65023..65895
                     /locus_tag="ACHA25_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8761492.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            66237..67109
                     /locus_tag="ACHA25_000154"
     CDS             66237..67109
                     /locus_tag="ACHA25_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8761493.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            67106..69064
                     /gene="nagE"
                     /locus_tag="ACHA25_000155"
     CDS             67106..69064
                     /gene="nagE"
                     /locus_tag="ACHA25_000155"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8761494.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(69396..70247)
                     /locus_tag="ACHA25_000156"
     CDS             complement(69396..70247)
                     /locus_tag="ACHA25_000156"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8761495.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            70370..70837
                     /locus_tag="ACHA25_000157"
     CDS             70370..70837
                     /locus_tag="ACHA25_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8761496.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(71100..71582)
                     /locus_tag="ACHA25_000158"
     CDS             complement(71100..71582)
                     /locus_tag="ACHA25_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8761497.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71682..72545)
                     /locus_tag="ACHA25_000159"
     CDS             complement(71682..72545)
                     /locus_tag="ACHA25_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8761498.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            73143..74018
                     /locus_tag="ACHA25_000160"
     CDS             73143..74018
                     /locus_tag="ACHA25_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8761499.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(74163..74315)
                     /locus_tag="ACHA25_000161"
                     /pseudo
     CDS             complement(74163..74315)
                     /locus_tag="ACHA25_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(74583..>75225)
                     /locus_tag="ACHA25_000162"
     CDS             complement(74583..>75225)
                     /locus_tag="ACHA25_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8761500.1"
                     /translation="TEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLI
                     QNMQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAK
                     LSVTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIP
                     WTTPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        21217 a        16040 c        13451 g        24517 t
ORIGIN      
        1 atcattctta catgctgaca gcaaagtcag gggcaaacca ttgaactgaa ccaactttta
       61 cgctttcgcc tggttgagga gcatggatat attgtcctcc gcctaaagca atcgctacgt
      121 ggtaagttcc gccttgtgac ccccagaata acaagtctcc agcttttgct tgagaaactg
      181 aaatttttgt tccagctgat tcttgaggaa ctgtccaacc accgatgtca cgaccagtta
      241 cttgcatgta aacatagcgt gtgaaccctg agcagtcaaa gccacttgga tctttaccgc
      301 cccaaacata tggagtacca atatatttgt aagcttctgc tacgattgct gcaccattca
      361 cacttccaga aggagctgga gcaggtatta caggttttgg ttgttcaggt gttggtgttg
      421 gcgttggagc cggagtacta cttcctgtgc cgtttcctgt atcaacactt tgatctgttg
      481 atggtgttgt aggcgcaggt gttgttgatt cttctgttgt tgaagattca ggcactgttg
      541 ttgattcttc tgttgctgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt
      601 caggcgctgt tgttgattct tctgttgctg aagattcagg cgctgttgat tcttctgttg
      661 aagattcagg tgttgttgat tcttctgttg ctgaagattg tgtagctgat gatgattctt
      721 cagtcattga agattgtgta gctgatgatt cttctgttgc tgagctttgt gcagtagatg
      781 aagcttgagt agcttgtgct tcagcctctg cttgttcgcg tgcttctttt tcagcttttt
      841 cttgtgcagc ttgttgacga gcttgttctg ctagacgttg ttgttcacga atacgagctt
      901 gctctgcttc agcttctgct ttttgacggt tcaagtcagc tttcttgtct tcagcagttg
      961 cttgttcagc agctaatgaa gttttcagta cgttcaaatc agcttgttta gataaaagat
     1021 cgcctttttg agattccaaa gcagcttggt tttcagctaa ttcttttagt ttagcatcgt
     1081 tttcagcttt tttgtcttct acagcttttt tatcttgttt ttgttgttcc atcaagtcgt
     1141 tgtttgcttt taccatcgtt gtcattgctt gaacgcgtcc aatagcatct gctaatgaat
     1201 cagcattcaa tacagcatcg atgtagtttg agcttgtgtt gcttacttgt gcttcgcgtg
     1261 cttgtttttg gattgtatct tcacgttttt caatacgttc ttgtaaatct gcaatgtctt
     1321 ttactaattg tgcagattct tgacgcaatg tatcttgttt tgctaatagg tcttgtgctt
     1381 gtgtgttgat ttcagaaact tggctttcta aggcatcgat ttgtgattgt gcatctgctt
     1441 gttgattttt caagtctgca atcttttgat cttgctgttg tatctgagaa tcaaaatcgt
     1501 ccgccgcggc agcgatcggt gatgctacgg cagtcaaggt cattgaacat accattactg
     1561 ctgatattaa actctttttc actcttcatt cctccgactg gcttaattta ataaatatct
     1621 aaatatattt aagtctttct taacgatata aaaattgtac catagcagtg tgtcaacgat
     1681 ataatagtta tgttacaaaa atgtttcaaa acaactttta aaaatgacaa aactgttcgt
     1741 ttcagaaaat gatcatgaaa aagccgcgtt aagtctagca tttcttaaga taacaacgat
     1801 tctttttcgc tttcgaccta aaaataaaaa aagaaattat tcatcactga ataatttctt
     1861 aaagggaaaa atgaataata caaatataat catgttcaac agcagtgtag gtcctaaaaa
     1921 ccttgtaata aaatacgtgt tatttactac tgctaattta aaaatcaact ggaccaccat
     1981 cgtaaacaat tcataacccg tgacaaagat gatcatactg aaaaattcag taaagatgtt
     2041 gacatggata acgtctttca ttacatacat caaccataca attaaaggga gagctactgc
     2101 atagataccg ataacgccaa tatagtaagc gtcatagata gccccgagaa caatcgtggt
     2161 aatcagtaaa taacgttttt caaacgccat actgcaacat aataatgcca atatcagaaa
     2221 gtgggcattt gacatatagg tgcctttcga ccactcccct agcattcttg ttaaatgacc
     2281 atctatcaac atcaaaaaga acaaaacgat tggcagatag tacttcattg tttctttctt
     2341 caacatggac taatcctccg ctagccgttg gacaatcgtc acaactgaca cgtcatacat
     2401 ttctgcataa ggtttcacat aaacttcccg atccagccca taactatctg gtttcgtttt
     2461 aataaccgta ccgatcggca agttagctgg agaattccct ccaagaccgg atgtctggac
     2521 aacatcccct tctttgatat ccgtatcacc agttaattgg gtcaccacta aggcatggag
     2581 cttttcatca tagtttttaa gcaaaccaaa cgcttcgcca ttagccgaag atacccgtac
     2641 tggaaaatga ttggagcttt cattagagga tgacagtaat tcgattttag acgaagccgt
     2701 attgacctcg atcactcggc caatcaatcc tttttgcgac atgactgcca tatttgcttc
     2761 gataccgtct ttagaaccct tgtctacgat cagcatgtcc tgccaactgt caggcgaacg
     2821 agtaatgaca tttgccgtta ctttttcgta actagtcagc gtctgattca actcaagttc
     2881 ttttttcaac gcttcgattt ctctctcgta attcttgttt tgctgtgaaa cgtcatcata
     2941 gctatcgatt ttttctttta gccgctcatt ttcggcataa gttgaaaaaa gattatggac
     3001 agaatctact ccatttcgta cccaccgtac tggggcagag attgttttat caaccattgc
     3061 aacactgtca ttaacgatgg attggaagaa attgggcttt ccttcattcg ctcgctgcgc
     3121 agcggtcaca cttagtatcg tcaccacgat gataacaata accaaggtaa taataatatt
     3181 tttattagga ttgaacttct tcacaaagac acctcgaatt aattagactc ttgtgttcaa
     3241 ttttatcact aaatgccgtg aaacaaaagg taagttgctc ctcttaagct tttttttagc
     3301 aaataataga ttctgtcatt tttagcctga aagacaaatt tttttatttg tttatggatc
     3361 aatctttgct ttgaataact gcaatcatac catctgaaaa agaaaattct gcggtatctg
     3421 aaagaaattc gttacttgca taagatgtcg gatattctac ttctttattt tccaatacat
     3481 acttgctgcc ttttaatgta agattcgaga cgggagtcaa gcagcaataa gctaaatact
     3541 tcatatctgg tatctgtctt actatatgct ctccaggcaa ataataagac aaataatttt
     3601 gacaatctct gataataagc tgatgggcaa agggtttgaa gcgaggttcc atacctaacc
     3661 agagattggc taaaagatga tccaatcttc cgcccgtagc tccaatcaaa gtgacttccg
     3721 cttgcgggta acgttcaaat gtttttaaga tacctaactg tgtatctgta tcgtcttttt
     3781 ctgcagggga acgggtatac tctttggctt ttgcaaatac ttccttctgc tcttcgttcg
     3841 acaatgaatc aaaatcaccc actgccagat ctactgggta gcctgcttga agcaggtaca
     3901 gcgcaccacg gtctatcccg acataggcat cgtatttgga cagcagagac tgcggccagt
     3961 tttgcggatt acctccagca gctaataaaa ttttcattta gtctaatgcc tcttttaatc
     4021 gttcgatttg tccgactgga ttttcggctc catagatata tgatcctgca acgaagacat
     4081 ccgcccctgc atctttacag atttttgctg tttctggaac gattccgcca tctacttcga
     4141 ttgcataagt ccaattgttc tctctacgta gatcagcgag ttccgtaatt ttttctactg
     4201 tttcttcaat aaagctttgt ccgccgaacc caggattgac agtcatgact agtacttgat
     4261 cagccaaagg caaaacatgt ttgatcatgg agactggtgt tcctggattg ataactactc
     4321 cagctttaac tcctaagctt tttgtcattt gaagtgcccg atgaatatga ggagttgctt
     4381 cgacgtggac agtaatgata tctgctccag cttttgcaaa tgctggaatg tagttttctg
     4441 gatcaacgat catcagatgg acgtctaacg gtaatttagt aacaggacgg attgcctgaa
     4501 caatatttgg ccctaatgtg atatttggga caaatcggcc atccattaca tctacgtgga
     4561 tgtaatctgc gcctcctttt tccactaact caatgtctct ttgtaaattg gcaaaatctg
     4621 cactcaaaat ggatggtgct atcttcattc ttgtttcctc ctattttcgt ttaccttctt
     4681 tttttgcata cattggtttt cttttttcga tttcttgtaa gaattgcaaa taattgtcgt
     4741 aacgtgtctg tgcgatacca cctgtttcta ctctatgttt tacttcgcag ccaggttctt
     4801 tagcatgcat gcactctcta aatttgcaat ggacagaagc ttctacaaat tcaggaaatt
     4861 gtctcggcag ttctcctgct tcaattgtca aaaagtcaat cgagctaaat cctggtgtat
     4921 ctgctactaa cccgccatag ataggaatca gttccacatg acgtgttgta tgccgtcctc
     4981 ttccaagtgc atcagaaatc tctcctgttg ctaaattcag ttccggtgat atttgattca
     5041 gtagcgtcga ttttcctgct ccagactgac ccataaagac cgatagccgt tctgggaagt
     5101 aacggatcag ttctgtcgta tcacccgtat gattgggtac aatcaccgga tagcctattt
     5161 tagtataggt ttgcttgatc tcttcgattt tttctgggtc gtctgacaag tcggtctttg
     5221 tcaaaaaaat aatcggttcg atcgactcgt attccaaggt aactagaaag cggtcaagta
     5281 gattataaga aaatgcaggc tctacgatac ttgtgacaat gattccaaga tcaacatttg
     5341 tgactggtgg gcgaactaat tgattttttc tcggcaagat ttccagaaga tagccgtctg
     5401 tttgattttc actctcaaag atcacctgat ctccgacaag cggcgtgatt ttccgattcc
     5461 ggaaatttcc tcttcctctt gtttgatagg tctctccgtt tgcgtataca tagtaaaaac
     5521 cgctgattgc ttttctgatt tgtcctttga taagtgccac ctccgttttt gtttatttta
     5581 ccatacgaag aggaaagtag gtgttattcc atgcaaactt ctcaaagtat atggcttgaa
     5641 aattgatcaa aagagataga aacacaaaaa agagaatgca acaaatcaat gtcgcattcc
     5701 cttctgatat attttattca gcacttattt tgaactttct gaggtgctag ctgatgaact
     5761 tgtggagctg gaagattctt cttttgaaga gttgcttgtg ctactggagc tacttgagct
     5821 gctagaactg tctgaagagt ctttttcaga actagaagac gctggatctg gtcctttaga
     5881 gtaaattacg ttcacaacca ttcctttggt aattgtcgtt cctgcccctg gtactgtacg
     5941 aatcacttta tctttatcca ccgtgtttga atattcggaa gtctcatgcc cgatatattc
     6001 tgcaccgaca ctagctaaat aactttgtgc ttcgcttttt gtatagccgg agatatcatt
     6061 caatttaggc tctgttcctt ggcttacgac aaaattgatg taagtttctt ctgctacaac
     6121 ttcacccgag gctggatcct gactgatgat cgaacctgat ggtacagtgt cgctatagtc
     6181 ataagtagca ctaatattat tgatattgaa tccttgtgta acaagccaat tacgaacagt
     6241 ttcgtagttt tctcctacat gactactcaa agtcactgtt tctggtcctt tgctgactgt
     6301 gaaagtaatc gtgtctgttt taggatcgac ttctgtatct tctgatggag attgcgaaat
     6361 gatcattccg ctttctacct ctgaactgaa ttcctcttct ttttgaatca agtcagcaga
     6421 aaaccctaat tctttcaaac ttgcttcggc atcttcataa cttttaccac tgtaatcatc
     6481 taatttgatt tttttcgtgc cgctactaat atacagagtg acttcgcgtt tttgcttgac
     6541 tgtcgttccc gctgccgggt ctgtcttcac tacattacct tcttcgattt tgtcatctgc
     6601 aatttcttct gtttttgttg ctacttctaa tttcgctttt gcgagtgttt ctctagcaga
     6661 agcttcagaa agatcagaaa catcaggaac aacaacttct cctcgcccgc cagaagcaaa
     6721 gaatacagct gttccgattc ctaaaacagc aagaatagct aataggatca gccaccattt
     6781 tttctgtcct ttctttttag gatcaggcag aacttcttcc tcctctggta aggtttcgtt
     6841 ttctggcttg tcttttggca gaggcataga tttgaatgaa tcaggcattg gtgcatcttc
     6901 cgtaaggggt gtgataactt ttgtttcatt atccatcaca tgaggctgcc atttttcttc
     6961 gtttgcacga gctggtgata atactgtaga aagatcatca gacatttcct cggctgactt
     7021 ataacggtct gcaggttctt tagccgttgc ccgtaaaatc acgttttcca gtgcttgtgg
     7081 cacatttgga tcaagtgctt ttatagatgg aagatcatct tgaaaatgtt ttaaagcaat
     7141 tgttacagca gattcaccat caaacggtac gctacccgtc aacatctcat aaaggatgat
     7201 ccctaaagca taaatatctg actgcttcgt tgccatactt cctctggcct gctcagggga
     7261 cagataatga acagatccta gcatcgtatt cgtctgcgtg atcgatgttt cagataaagc
     7321 gatagcaatc ccgaaatccg tgatttttac aacaccttca ttatcgatca gtacgttttg
     7381 gggtttcaaa tctcggtgga tgatttgatg gttatgagct aaagaaatag cagaaaggat
     7441 ctgctgcata atatttactg ctgtttcata aggaacagga taatgtgttt ggatatatcg
     7501 tttcaagtcc attcctttga cgtattccat caccagatac tgcatgttgt cttcttcacc
     7561 aacatcgtaa acactaacaa tgtttggatg aacgagttct gttgctgcta aagcttctct
     7621 ctgaaatcgt cgaatcgcag tctggtcatt ttgaaagtca aatcgtaaga ctttaactgc
     7681 tacatcacga tccaagatta aatcgtgcgc caaaaataca tttgccatac caccgctacc
     7741 gatatttcca gtgatttgat aacggccatt taattttttg cccaactcga tcatacactg
     7801 tcctccttat attcgataag aagaacggtg atattatccg ctccgcctgc ttcattggca
     7861 cgatcaatca gctcagttac tttatgagac aatgggcctt cttgattgat gatcgttcct
     7921 atttcttctt cagacagcat gttcgtcaag ccatctgaac ataacagcag tcgatctttt
     7981 agttgccaaa gatgagtaga aacatctact tcgactgttc caggcatgcc gacagaacgt
     8041 gttaaaatat ttttccttgg atggttcact gccatttctt cgctgatttc tcctgatttg
     8101 actagttcat tgactaaaga atgatcctcc gtcaattgca tgattttatc ttcacgaatc
     8161 aaataagctc tgctatcccc aacattggca atcgtgaacc agtcatctga cagagcggcc
     8221 gctacgattg ttgttcccat accgttatat tccggctgtt cttgtccacg ctgataaatc
     8281 agcatatttt cttcttgaat cgattgaatg aaccattggg cgattttttc atcgtcaaca
     8341 gcttcttgtt tttcccaagc ttcgcctaga ttcgttaccg ccatctgact ggcaatgtct
     8401 ccggcacgat gaccacccat cccgtctgct aatacagcaa gcttgagacc tgaacggctg
     8461 gtaaatacac tggcatagtc ttgatttgtg ttacgtcgtc ttcctacatc tgattgatat
     8521 tcaatctgca ttttttcacc tcattacttt ttgcgcaaac aacaaatgaa aaatccatct
     8581 gtgtaaaact gatgcggata caaagtcaac attttctctt cgattgatga ttgtagcata
     8641 ggattggtca aaacatcaat cctctcaaaa tcagggtgtc tttccaagaa agcagaaaca
     8701 acgtcttggt tttcttcttt taagatcgta caagtgctat aagtcagaat accggaagac
     8761 tttaaagttg gtgcgcagct ttctaaaatt tccaattgga tttttggtaa attcgctaat
     8821 tcatccggct gttttttata acgaatatct ggttttcgcc gaagcagccc aagacctgaa
     8881 caaggcgcgt ctactaaaat ccgatcaaac tgctctgctt cgaactgttt actgacttct
     8941 ctagcatcca tcttttgagc gtagacagca gaagagacac ctagacgtgc tgcattatct
     9001 tcaatgagtt tgactttgtg ttcgtgtaca tctaaagcga ccacttctcc accgcatgac
     9061 ggatctaaaa aagaagcaat atgcgtcgtc ttacctccgg gtgctgcaca ggcatctaag
     9121 acatgatgtt caggctcgat ttgcatagac ggcgctacaa gcatcgaact ttcatcttgg
     9181 atcgtcatga cgccatcgtg gaacaatgag ctagaagcta aatgcccttt tttcgctact
     9241 ataccgtatg gactgacttg gcttttttca gctgtgatct cttcttcttg caaaacagtg
     9301 atggcctgat ctctggaaag tcggcgtgta tctactcggg cactagcatg gctaggctga
     9361 tatagcgata aaccgagctt ttcagtttct tcgtatccga tttgatccac cagtcgtttc
     9421 gtcatccata gaggcaggct gattttgaca gaaagtcttt cgataggatc agcaatgcct
     9481 tgcagatctg gtgctccttg acgctgataa ttcctcaaga caccatttac aaatttccct
     9541 gtcccaggat ttccttttac ttttgcgatt tcgaccgcct cgtttaaaat agcatgtgca
     9601 gggactttat ctaaaaattc taattggtat acagaaagat aaagaagcat cttgacccag
     9661 tcatccactt tttttgcttt agcgatcaat ggtgcaacgt aaaattccaa tagtcgctga
     9721 tggctaatcg taccatagat caattctgta aataaaccca catctttttg atttagctcg
     9781 ctttgtttga tcatctcatt caacaaaaga ttcgagtagg cgccaccctt gttcactcgc
     9841 tctaatgtca gtaatgccat atatcgtact gattttttca tttttttagg gatttttttc
     9901 attatttcac ctgctgacca atctgaactt gctgaccgct tccgttaagg aaatcatgta
     9961 cggcctgttt tcctttacca gctggctgta attcattgat ggctaagacg gtctgtttac
    10021 cgcaagcaat ccatagattt tttttgtctt tccggatgat cgtaccaggt gctttgtctg
    10081 tagtttctgc caatacttca accgataata atttccagcg tatgccctca taggtcgtga
    10141 atgcaattgg ccaaggacgc attccgcgta cttggttgtc aatttcttct gcagttttat
    10201 gccaatcgat tgcttcttgt tcccgtgtga tgtttggcga aaaagttgct tctgactcgt
    10261 cttgaggtac tggtttttgt ccatccaaaa tattgggcaa tgtttctagc aatagctttc
    10321 ttcctaaaag gctaagcttt tcaaacatcg tccctacatc atcttgttta gtgattggta
    10381 tgctttcttg cgcatagatt ccgcctgcat ccattttttt gatcatttcc atgatcgtta
    10441 caccagtttc tttctcccca ttcatgatgg cataatgaac aggcgcacct ccccgatatt
    10501 taggaagtaa agaagcatgg acattgattg cccctaattt tggtacttgc aataactttt
    10561 caggcaagaa ttggccaaat gccgcagtga cgatcacatc aggcaccaat tcaatgattt
    10621 cttccatttc tggcgaaccg gagattttct ctggctgcag cacacggata ccgtgcttca
    10681 gagccgcttt ttttaccggt gtcggcgtaa ttactcgttt ccgcccaact ggacggtcgg
    10741 gctgtgttac cactgcctgg atctcgtaac ctgattctat cagcccttca agaatcggca
    10801 cagaaaaagc aggtgtgccc ataaatacta gcttagtcat caagatgttc ctccatatat
    10861 tcttcaagtt cttcttccgg gatctgctgt atcattttct caatgaacaa gataccgtct
    10921 aagtgatcga tttcatgctg gaaggcacgg gctaaatagc caaaagccgt cacttctatc
    10981 tcttctccgt ctcgatcata ataacgcacg gtcacttcat ctgctcgctt gaccgttcca
    11041 tagacatgcg gaatactgag acaaccttct acgtcaaggc tttcaccttt tgcttcgatg
    11101 atttctgggt taatcagttc aaatttctcg ccttcatcta cttcgatcac agcaattctc
    11161 ttgttttgtc ctacttgagg agcagcgatc cctattccgt cattcgcaat cattgtctca
    11221 tataaattgt ctaataaagc gattgtctcg tccgtgatta catcgattgg ctgagcagtt
    11281 ctttttaatt tatcattagg gtggatcaat ataggatagc gcataatttg gccctttctt
    11341 aaataaagtt taatggttcg ttgtcaattg ccacaaaaag accttgtcgc tgttctttct
    11401 ggcttccatt caaaatttca tccaaaacat gaggaagatt cggttcatgt ttatatttga
    11461 tgattagttg ataataatat cggtttttca ctctcaaaat catgctgggc gttggcccta
    11521 atagcaagct ttgagggctt aacacttctt ttagctgatt ggcaatctga aagattttct
    11581 ttgccgctac ttgttcttca ggatggctgg cggttatttt cacagtaaag taataaggag
    11641 gatagcctcc acgatgccgt acatgcattt cctgctgata aaactgctca taattttgtt
    11701 ttttagccaa ttctatggcg taatgttgag gattgaatgt ttggatcacg acttcgcctg
    11761 ctttttccgc tcgccctgct cgtcctgaaa cttgggtaag caattggaat gtatgctcac
    11821 tagaacgaaa atccggtaaa ttcagggagg tatcagcatt caacacccca actaatgtca
    11881 cttctggaaa gtccaaacct ttagcaatca tttgtgtccc taataatata tctgcttctt
    11941 ttgcaccgaa tttttgtaaa atctgttcgt gtgccccttt tcttcgagtg gtatctacat
    12001 ccatacgcaa aatcctagct tgcggataaa gttctcggag ttcctcttct actttttgag
    12061 tacctgttcc atagtagcgg attttattgc cgccacaatt aggacagcga ttgggaatag
    12121 gttcttcatg tccgcaataa tggcagcgca ttgaacgtgt atccatatgc agtgtaagag
    12181 agatatcgca attaggacat ggaagaacaa atccacagtc cctacacatc acaaaagacg
    12241 aatatcctcg acgattgagc agtaggacag tttgttcttt tttgtctaat cgattttgta
    12301 ttttttcctg caaattagca gaaaaagtag aggtccgatg attctgaaac tcttcacgca
    12361 tatctacgat ctcaatagct ggaagctgtg cagctgcttt ggctcggtgg tttaactgca
    12421 ataactgata aacgcctttt tgagctcgtg ctcttgattc taaagagggc gtcgcgcttc
    12481 ctagaactat tgggcaatga tgatatttac tgcgccaaat tgctagatct ctcgcatgat
    12541 aacgcggtgt ctcatcttgt ttgtaacttg attcatgttc ttcatcgata atgatcagcc
    12601 cgatattttt cagcggggca aaaatagcag atcgtgcgcc tactactact tgcgcttctt
    12661 cccgctcaat cttgcgccat tcatcatatt tttcaccttg ggaaagtccg ctatgtaaga
    12721 ccgcaactga ttctccgaag cgtcctttga atcgctcgac catttgcgga gtcaatgcga
    12781 tttcaggcac taacataata gccgttttat tttctgacaa aacatcagcg attgcctgaa
    12841 gatatacttc tgttttcccg ctgccagtga ttccctccag caaaaagacc tgatcttttt
    12901 gctgctgacc tgctgtgatg atttgttcga cagcattttt ttgttctgca ttcagttcca
    12961 atgccgtcgt ttggtcaaat actcggtctt tataagggtc gcgataacgt tctgattcta
    13021 taaactccag ccagcccttt tcttttcctt gattcaagac agcagtactg attcctttat
    13081 ttttgaaata agcaattggt tcttcttggc tcagttcaga taaacacaaa agaagttctt
    13141 tttgtttctt ggcattttgc cgtaattttg cccattcttc ttcgatttgc ttttcagtta
    13201 acaaagaacg gaccaaacga atcgttttta ctttatttcg tgtatggact tcatatttga
    13261 tatctactaa ttgctcttgg cgccagcgaa tcaattgaga aaggctgcct tcttcttctg
    13321 cttccttcca ggaaatcact tctcgctgat caaacaatac ctgaacttct ggtgtgtctg
    13381 ccaaaggata gatcaattta tcatattctg ctttcatcac actcggaagc atcgtttgta
    13441 gacaagtgat tttgaatgcg taagtaattt ttttcatata atcagctaat gaaagtaatt
    13501 cttcattgac caccggagca aggtctagca aacgaataac tgccttcaaa gatggattag
    13561 ctgattcttc tgatttttgg atagccataa caaatccttg gacatgacga ttgccattac
    13621 caaaagggac ttctactcgc atcccgactt gcaaagctgt ttccatttca gagggaacca
    13681 aataagtgaa tggctgatcg gtctgcatag taggtacgtc cacaatgact tcagctgtta
    13741 atgtcatggt tcgtttcctc ctttttttgt cttttcttat caagcgacaa aaagttcctt
    13801 gcctatttta ctagagatcg atggatttgt cttatcttct gccaaatcct aacaaaaaat
    13861 taacagcaga taagaaaaaa aagaactgtg tcaaaaagtt cagacacagc cttttctacc
    13921 agacaataag tggcgaatgt ttgaaaagga aacagctggg tttgcttttc tttttagatg
    13981 ttcgctctat ttttctttat tacatttttt gctcttcacg gatacgtgat tccaattcgt
    14041 gttgttcacg ctctttttgt gcttgacgtt cctcttcttc catcttcaaa cgttcacgtt
    14101 ttaattcagg atgtggatca ttgactacat ttcctgcatc gatttcttct aaagcttgac
    14161 cgacgctttt tactgaatca aaagaatcta gagtaggctg tgcacttgca tccaattcat
    14221 gcgcgcgttt acttgctaaa atcactagag aatattttga atttacacga tctaatagtg
    14281 agtcaattga aggttttaac atcataagac tacatctcct ttaacatttt aatatattta
    14341 ccgattacgc gatctactcg aaaatgttca ctggcaataa tgtttttgat tcgttcaact
    14401 gctagcggca cttcatcatt tacaacagca tagtcgtaaa gagccatcat ttcgatttct
    14461 tcttttgcaa ctttcattcg tttttcgatt acttcatctg catctgttcc tcggcctacg
    14521 atccgtgatt ttaattccgc taaatctggc ggagtcaaaa agatgaatac accatcgggc
    14581 actttttctt tgacttgttt tgctccttgg acttcaatct ccaagaaaac atcttttcct
    14641 tcatccaatg tcttgttgac atatgacaaa ggagttccat aataattccc gacatattct
    14701 gcgtattcca gcatctctcc agcttcgatc attgattcaa actcttcttt gcttcggaag
    14761 aaataatcaa cgccttcgac ctccccttca cgcattttac gggtagtcat tgagatcgaa
    14821 tactgaaaat cattatcctc actctcaaaa attgcttttc gtactgtgcc ttttcctaca
    14881 ccggatggac cagacaacac gattaataat ccacgctctg acatgacgac gtcctttcaa
    14941 ctagtctttt tgtacttatc gtttattttg cacttttttt ctgcaacttt caaggttatc
    15001 tttaattata gctagaaatt ggtaaaaaag ctaccctgta ccaagaggtc gtaaaaaagc
    15061 tgtcctgcat caagtaataa gaacagccaa acataaacag cttctcatgt ttttcgtttg
    15121 gttggggtta ttatcgcaga tgcaagcttt tgaacgccgt gtataagagg tcgtgaagaa
    15181 gccgattagt ttcgagtatg aagaaaaatt ttgtgaaaat agtttctcac atttttgcat
    15241 aaatttggct taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag
    15301 attgtgacac gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg
    15361 aagaaatcca attctgttta tttattgtat ttctttgttc agcatccttg ctaaaagccc
    15421 atattggaca aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct
    15481 gtcggatctt ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg
    15541 taagcaccgt cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct
    15601 ttccatacgt caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca
    15661 cttaattcgt ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa
    15721 tattttgtac ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt
    15781 taatttcgat tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga
    15841 tcaggaaatc taattcccga cctacaggac attgtgcttc gataagttca tctagttttt
    15901 gtacatggat cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg
    15961 ctagctccgt caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt
    16021 tttgatacct tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta
    16081 gtacttcttt gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt
    16141 tttctcgact cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg
    16201 ccaattcttc catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagca
    16261 cgaagcgttc cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa
    16321 ttccgtctgt caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt
    16381 tttcgttttt gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact
    16441 gctgtttaat aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta
    16501 tttgaacatc tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt
    16561 ttgtttcgcg gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct
    16621 cattacatat tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg
    16681 cctttcccca ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga
    16741 ccattccagc tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata
    16801 gcgatttctt ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat
    16861 aatataaata taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac
    16921 gatccttgcg ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt
    16981 gtattttttg ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt
    17041 gtgccaataa atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca
    17101 tgtctacttg atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca
    17161 tcgccaatcc gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc
    17221 aaaattttat gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca
    17281 aaaatcctcg ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat
    17341 agtaatagcc taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc
    17401 tttggctatc aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga
    17461 taaaaatgtc tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa
    17521 atgcctctgt tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac
    17581 cgtctgcata tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag
    17641 aggcgagtat ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt
    17701 ccgctagttc tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga
    17761 gtaaggacaa atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc
    17821 cttcaattag tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt
    17881 cttcaactgc agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata
    17941 aattcacagc ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg
    18001 aggtacctgt gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa
    18061 tctgataagc accgtgcgct cctcctctac ctaaaacaag tgctgtatga tagaccagct
    18121 ttttatttaa ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt
    18181 ctgatagagg agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag
    18241 cttccatata ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat
    18301 taaaaataac atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata
    18361 ctgctttaaa agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat
    18421 gactttgaaa aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt
    18481 tctgtgaaaa gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca
    18541 gtacgtcata aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa
    18601 ttcataaaac ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag
    18661 agtgttttca atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt
    18721 tttaatgcaa atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg
    18781 actgagtcag tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg
    18841 actgctactg actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat
    18901 ccatctgctt ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca
    18961 gaaaatattc agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag
    19021 tggatcgact ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa
    19081 ttattatatt ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag
    19141 aataattttg tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta
    19201 gatgaatttt ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat
    19261 gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg
    19321 aaagaaatgc gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg
    19381 atcgatggta aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa
    19441 ggcacccggt atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga
    19501 cactggcaat tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc
    19561 ttatcaaaaa ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc
    19621 ttctttttta tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt
    19681 tggatcccta ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc
    19741 attacgaaag aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc
    19801 actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg
    19861 atgctttttc taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc
    19921 atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg
    19981 acacttaaat aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc
    20041 agcatccctg cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat
    20101 gaaatagaaa aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct
    20161 gcaacaatcg ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg
    20221 aataaaaaag tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca
    20281 caattcctac gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc
    20341 agcccaattg gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg
    20401 gactaactgg tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac
    20461 ttacttcatc gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa
    20521 ctagacgctc gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat
    20581 gtgtacatga agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg
    20641 tgatcgtacc tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt
    20701 gtccaatgat attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg
    20761 caatcggaga tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg
    20821 gtgaaacaac gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa
    20881 gtggcgcacc catcaaatga tcgactggaa tatcaaagaa accttggatt tggactgcat
    20941 gaaggtccaa agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt
    21001 tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag
    21061 gcatgactac gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca
    21121 actccatcaa gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc
    21181 cacgaatact ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg
    21241 attttcctaa ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat
    21301 ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt
    21361 tctttttcac aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc
    21421 aagtgatttt gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat
    21481 tgacttgtct tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg
    21541 acccggcagc aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag
    21601 aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga
    21661 cgaagacaac accacatccg acattgattt cttctcccag cgtagcatca ccaacataag
    21721 tcaagtgacc gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc
    21781 cgatatggac gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat
    21841 ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct
    21901 caatcctact gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg
    21961 tatttccttg gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg
    22021 catcgatgta tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt
    22081 taattcgctt tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca
    22141 ttgattcttc aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga
    22201 tttcgatgat atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta
    22261 acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa
    22321 gagcttcttc tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt
    22381 cacggatgat tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac
    22441 ttgcattttt tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg
    22501 gtgtatcgcc actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt
    22561 gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc
    22621 ctagttgtgc tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt
    22681 tcgtttcact gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta
    22741 aaactttata taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat
    22801 aacgtgcgtc caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta
    22861 tacccatttt ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa
    22921 aatagataga ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc
    22981 ggtcacaagt cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg
    23041 acttagagtt aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc
    23101 ttcggtatta gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta
    23161 atactcagga caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt
    23221 tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct
    23281 tactactcga aactaagcgg ctttttcaca acctcttatt ctgaaaaata atttcctggc
    23341 acaaccgtaa tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa
    23401 tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt
    23461 tctgattcga attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg
    23521 aaatcatcca ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc
    23581 ttttcaattc tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt
    23641 atttttgagt cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg
    23701 gcaataggta cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat
    23761 ttagaagcaa tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag
    23821 agataaacat atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat
    23881 tcaatatatt ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca
    23941 gcacccggga ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt
    24001 cccaaatctt cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag
    24061 gaaatcaatt catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg
    24121 cgacgtactt tcacctattc caactccttt taagaaattt tatcgtttta cattatataa
    24181 gtttttcttc aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt
    24241 aaaataaaaa gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat
    24301 ttagttattt tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa
    24361 caaagcaata aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct
    24421 agttagtcct gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt
    24481 gaagcttttg cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa
    24541 aagtgcaccg gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat
    24601 catcgacatc aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag
    24661 aacatacatc ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc
    24721 taaaaagatg acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat
    24781 acttgttgct gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc
    24841 agccattaaa atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc
    24901 caatatcaca gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa
    24961 aaagaatcct aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga
    25021 ttgtcttctg atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc
    25081 ggcaagaaac gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt
    25141 ctcaccagtc ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc
    25201 attagaactg atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt
    25261 ctggattctt catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta
    25321 gcaaaaatac gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc
    25381 cgatttcgca tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct
    25441 ttttcagaga gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg
    25501 atcaccgtac taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata
    25561 cttaattttt ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc
    25621 agtaatccta acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa
    25681 atgacaaatt cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca
    25741 tagtaaaagg tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac
    25801 ttaattgata ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc
    25861 gcctgctttg atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac
    25921 tcctgtttca ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat
    25981 cactttgatg ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg
    26041 ttcctgatct ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa
    26101 ataagcaaaa gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc
    26161 ggagtatttt tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa
    26221 agtttccttt ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt
    26281 tatagctaat accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc
    26341 ttcttctgat tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc
    26401 tgcttcgatc atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa
    26461 ttccatgtct gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt
    26521 tgcacttggt tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc
    26581 ttcatcccct acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt
    26641 caaatcctct tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc
    26701 acctatcatt accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa
    26761 caaatcggaa ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt
    26821 caatagaaat ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg
    26881 gatgagttgc tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt
    26941 taggattcct cagtttttca tcacctattt tacttcttat attttattgc agggttcgta
    27001 ctaaataaac ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat
    27061 aatgttgaca caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc
    27121 cgtacttgat catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat
    27181 cttctaaact gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt
    27241 cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg
    27301 tacgtgtaga gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag
    27361 gtaatacttg gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt
    27421 aaggaacatc tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca
    27481 gattccataa ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta
    27541 atccagctgc tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt
    27601 atttattttt tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa
    27661 atgctttatt tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat
    27721 ctgccaagtc tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat
    27781 ataatacatc cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct
    27841 cacccattcg aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc
    27901 ttatgtctgc ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct
    27961 tcataatcca aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac
    28021 tcggaacatt tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga
    28081 aattacttcc tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt
    28141 tcttttcata agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca
    28201 tgactttttc ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc
    28261 ctagattggc ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat
    28321 agtcagctaa ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct
    28381 tcttcgtcat cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac
    28441 cgacaccgct agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc
    28501 gtgcaccttg aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca
    28561 gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct
    28621 gactcagccg agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg
    28681 tgaagtgaaa acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg
    28741 caagctgata gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca
    28801 ttttaggatt gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac
    28861 caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc
    28921 gaagctgatg gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt
    28981 tttcgtactc actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta
    29041 aagctagcaa tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa
    29101 acaaaataaa atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg
    29161 caattgcgta aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc
    29221 aagaataaac gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa
    29281 tcaagacata taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac
    29341 caaaagatag taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc
    29401 tagcaaggcc gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga
    29461 atgagaacgg caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg
    29521 atagaagtac catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa
    29581 ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat
    29641 gtgtgatgaa caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat
    29701 ataatagcca aactactgct acatcttgcc gaacagacaa ttgactcatt cctaagatca
    29761 tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag
    29821 caatcatgaa cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga
    29881 tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa
    29941 gcaagccttt tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga
    30001 aaatgactgg tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac
    30061 tttctgactg aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag
    30121 tcagcataaa tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct
    30181 ttccataaaa actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt
    30241 tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc
    30301 cttgtttttc taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca
    30361 gcagtccagc cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc
    30421 aaatatcttg ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta
    30481 acacggagcc gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca
    30541 tcaagtatcg tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac
    30601 cccaatgtta tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg
    30661 atagaaaaag aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc
    30721 ggcacaggga atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg
    30781 aaagaagagg tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa
    30841 actggcgtcc atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt
    30901 ttagccgtga aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag
    30961 aaagtcttat tcatttctat tgcttcagga aaatcattaa tagatttaga aacagcgctt
    31021 gaagatcctc aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca
    31081 ggcgtttctg cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg
    31141 atgatttttg aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc
    31201 attggccttg ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg
    31261 actggtgtac tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt
    31321 atgggttctt gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa
    31381 gtttcttctc ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc
    31441 atcccagcaa tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat
    31501 aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt
    31561 gccgcggctc ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg
    31621 aataacgatt actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat
    31681 atcacgtact aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg
    31741 aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat
    31801 attttccaga cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg
    31861 tcttgctgct gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc
    31921 cacatctcca tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc
    31981 agctaattcc gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc
    32041 aatatcatgg agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc
    32101 taagatacca gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact
    32161 agttctgaat cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc
    32221 gacttcaaac gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga
    32281 tttctcgacc atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc
    32341 caaggtcatc cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc
    32401 cggtgtatca tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg
    32461 accttctcgt ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac
    32521 agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt
    32581 cgctttgcga tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa
    32641 ctcatgattc agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga
    32701 taaaccagca attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt
    32761 ttctcgctct tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga
    32821 gttctcgcgt ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa
    32881 acgattctct tgagatttta attctaactt actttctctc agctcacttt caacttcaga
    32941 acggtatttc tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc
    33001 ttcttttttt gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt
    33061 ctcgtgacgt gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat
    33121 agcgaggaga atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc
    33181 atctatacag cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat
    33241 gtgtacaaaa aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa
    33301 ataaacaaag aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca
    33361 ctagtttcat ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta
    33421 gatgaacaga tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct
    33481 ttattcaata aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt
    33541 gtttatcata atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct
    33601 gggacagcga cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc
    33661 atcatttcag gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga
    33721 tcttctttat aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg
    33781 tctagaagtt ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt
    33841 ttgaaaggag gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata
    33901 tctgtacctt gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat
    33961 ttcaatgcgc gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca
    34021 cggatttggt tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta
    34081 cgcaatgctt gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg
    34141 tcgatttctg ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc
    34201 ccacttgata ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa
    34261 agaagtagtt catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc
    34321 tcagcatcaa tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt
    34381 gcaactgttg ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg
    34441 taaccgccga cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt
    34501 tgttggtcga ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt
    34561 tttttcagtg cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt
    34621 atatttacaa atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa
    34681 gcaagaaaaa accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa
    34741 agatcaaatc cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga
    34801 tatttttccg aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat
    34861 tgaccttcta acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt
    34921 gctatctcac gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat
    34981 tctgctaata gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta
    35041 acgaatccgc ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag
    35101 gcgccggctg tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc
    35161 actacgactt ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt
    35221 tcttggattt tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt
    35281 ttcactgtca gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg
    35341 atctgtgtct caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa
    35401 aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga
    35461 cgtacttgtt ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac
    35521 gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga
    35581 accccgcctt cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt
    35641 gtttcaaaat atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg
    35701 aggtgagccg cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat
    35761 aagagtatga gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga
    35821 ttatctccta caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac
    35881 aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt
    35941 atttctgctt tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt
    36001 ttatttaaaa agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa
    36061 taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa
    36121 agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc
    36181 cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca
    36241 cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac
    36301 cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag
    36361 ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta
    36421 aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg
    36481 cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg
    36541 ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta
    36601 accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca
    36661 gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga
    36721 aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga
    36781 ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt
    36841 tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa
    36901 gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat
    36961 gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac attttttgga
    37021 ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca
    37081 ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc
    37141 tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct
    37201 aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata
    37261 tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca
    37321 cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag
    37381 gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt
    37441 ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca
    37501 accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc
    37561 tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat
    37621 cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca
    37681 gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa
    37741 tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca
    37801 tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa
    37861 gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa
    37921 agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa
    37981 cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct
    38041 gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt
    38101 aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag
    38161 atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc
    38221 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta
    38281 agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
    38341 cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat
    38401 ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa
    38461 cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat
    38521 ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt
    38581 ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg
    38641 gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca
    38701 gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt
    38761 cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat
    38821 tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc
    38881 ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg
    38941 tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca
    39001 aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct
    39061 aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct
    39121 tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct
    39181 tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct
    39241 tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca
    39301 ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag
    39361 cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg
    39421 atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta
    39481 gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt
    39541 tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc
    39601 tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa
    39661 gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt
    39721 tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata
    39781 aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca
    39841 tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca
    39901 ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc
    39961 ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg
    40021 tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc
    40081 ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt
    40141 tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg
    40201 aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa
    40261 tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt tctctctgac
    40321 attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc
    40381 aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg
    40441 aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa
    40501 cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc
    40561 tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc
    40621 ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa
    40681 ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc
    40741 aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa
    40801 gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt
    40861 taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa
    40921 actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct
    40981 tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccgaat aattcaaact
    41041 atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg
    41101 cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag
    41161 attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc
    41221 ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta
    41281 taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt atagcataga
    41341 tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt
    41401 ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag
    41461 agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt
    41521 ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg
    41581 tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc
    41641 cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga
    41701 tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct
    41761 ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca
    41821 tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa
    41881 atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt
    41941 cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg
    42001 aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc
    42061 ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat
    42121 gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac
    42181 aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat
    42241 cttcccatca gtatcttgaa aaaacgtatg ccaagcttct ccatcaatgt catcaacttc
    42301 ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta
    42361 aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc
    42421 ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga
    42481 atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga
    42541 gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa
    42601 tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc
    42661 tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc
    42721 ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat
    42781 ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag
    42841 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
    42901 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
    42961 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
    43021 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat tgctaaaggt
    43081 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
    43141 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
    43201 atttgattgc ttataacgct tttataataa cttccaaagc taacaccttt gcttttttcg
    43261 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
    43321 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
    43381 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
    43441 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
    43501 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
    43561 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
    43621 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
    43681 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
    43741 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
    43801 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
    43861 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
    43921 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
    43981 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
    44041 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
    44101 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
    44161 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
    44221 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
    44281 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
    44341 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
    44401 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
    44461 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
    44521 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
    44581 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
    44641 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
    44701 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
    44761 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
    44821 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
    44881 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
    44941 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
    45001 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
    45061 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
    45121 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
    45181 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
    45241 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
    45301 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
    45361 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
    45421 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
    45481 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
    45541 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
    45601 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
    45661 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
    45721 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
    45781 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
    45841 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
    45901 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
    45961 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
    46021 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
    46081 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
    46141 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
    46201 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
    46261 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
    46321 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
    46381 tgtagcaggt tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
    46441 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
    46501 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
    46561 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
    46621 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
    46681 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
    46741 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
    46801 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
    46861 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
    46921 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
    46981 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
    47041 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
    47101 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
    47161 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
    47221 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
    47281 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
    47341 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
    47401 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
    47461 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
    47521 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
    47581 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
    47641 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
    47701 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
    47761 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
    47821 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
    47881 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
    47941 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
    48001 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
    48061 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
    48121 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
    48181 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
    48241 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
    48301 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
    48361 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
    48421 gcaacagcaa aattataagt tggcatacca tcgcgttttt ggatgacaaa gtcgccaccg
    48481 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
    48541 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
    48601 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
    48661 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
    48721 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
    48781 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
    48841 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
    48901 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
    48961 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
    49021 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
    49081 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
    49141 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
    49201 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
    49261 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
    49321 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
    49381 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
    49441 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
    49501 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
    49561 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
    49621 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
    49681 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
    49741 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
    49801 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
    49861 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
    49921 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
    49981 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
    50041 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
    50101 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
    50161 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
    50221 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
    50281 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
    50341 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
    50401 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
    50461 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
    50521 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
    50581 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
    50641 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
    50701 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
    50761 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
    50821 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
    50881 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
    50941 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
    51001 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
    51061 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
    51121 aatggctatc ccatttgttt tagcggtttt gagtagttct gcagtggttt ctcttacttg
    51181 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
    51241 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
    51301 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
    51361 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
    51421 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
    51481 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
    51541 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
    51601 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
    51661 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
    51721 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
    51781 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
    51841 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
    51901 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
    51961 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
    52021 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
    52081 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
    52141 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
    52201 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
    52261 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
    52321 acttcaaatc ctctttttct caccggtttt cctccctaaa taaactactc ctattctaac
    52381 atatccgagg ctgtaaaaga ctttctttac aaattcttca agaaattctg ctatcttaaa
    52441 gtaaaccatt aattcgttct ttttccaaac gactgcaagt tgtacaaaaa aactattttt
    52501 tacatgactt tactcgtcaa aacaagtaga atagaacgaa attaatgagt ggatatttga
    52561 ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac gaattattgt caaagtagga
    52621 acaagtacat tgatttatcc caatggaaat atcaatcttg cggcaatcga tcagttagct
    52681 tttgtccttt ctgatcttag aaatcgggga aaggaagtga tcttagtctc atcaggagct
    52741 gtaggcgttg gtatgcataa attacatttg tcagaacgtc cgactttgat tcctgaacag
    52801 caagctgcgg cggctgttgg tcaagccgaa ctgatgaata tctataatca aagattcagt
    52861 gtatataatc aacagacggc acagatcctc gtgacaagag acgtggtaga ttatcctgaa
    52921 agtcgtaaaa atgtgaggaa tacattggaa cagcttttga ttatggacac gatccctatc
    52981 gtgaacgaaa acgatactgt agcagtggat gaattagatc atctgacaaa atttggcgat
    53041 aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt
    53101 tctgatatcg acgggtttta ttcagataat ccgactacaa atcccgaggc tcagatgtat
    53161 cgctatatcg ataaaatcga tgaaaagatt ctggcacaag caggtggtgc tggtagcaca
    53221 tttggaacag gcggtatgca gagtaaatta aaagccgctt ctaggatatt tgaagcaaac
    53281 agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta tttttgatat tcttgcagga
    53341 aaagaagtag gtactttatt taaggaggac ttgaaatgac tgacttgatg caattaggac
    53401 aacaggcaaa agatagcgcg caacaactag cacttatgcc tacaaaccgc aaaaatgagc
    53461 tacttgttca aatggcacaa accatcaaaa agaatcaaca agcgatcatt gaagcaaatc
    53521 ataaagactt agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc
    53581 agctgacacc tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt gcttcgttag
    53641 acgatcctat tggaaaagta gatgagatgt ggacgaacac cgacggattg agaatcggca
    53701 aaaagcgcgt gccactaggc gtgatcggca tcatttacga atctcgtcca aatgtcacga
    53761 cagatgcagc tagtctagca ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag
    53821 aagcattctt ctcgaatcaa ttattagtcc aattattgca gcaagtactg gtcagtgctg
    53881 gcgaatctcc ttatgccatc cagtttgtcg atgatacttc tcatgaaact gcaggacagt
    53941 tgatgcgatt gacagaatac ttagatgtgc tgattcctcg aggcggagca aaattaatcc
    54001 aacgagtcaa agaacaggca acggtaccta tcattgaaac aggtacagga aataatcacg
    54061 tatatatcga taaagaagca cagttatcta tggcagtgaa catcatcgtc aatgcaaaag
    54121 cttcccgtcc atctgtctgt aacgcggcag aaacattgct gatccattct gagatcgcac
    54181 cttttttctt gccagctatt gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg
    54241 atgctcgtgc gttagaatat ctagagacgg ctgctttagc agaagacaca gattgggata
    54301 ctgaatatct agattatatc cttgctgtca aagtggtcga ttctttggat gaagcaattg
    54361 cacatatcaa tcggcataat actaaacatt cagaaacgat cgtgacagat agttatgcgg
    54421 ctagtcaacg tttcttaaat gaagtggacg cggcggctgt ctatgtcaat gcctccactc
    54481 gttttacaga cggatcggta tttggttttg gtgcagagat tggtatctct actcaaaagt
    54541 tgcatgcccg tggtccaatg gggttaaatg aattaacttc cacaaaatat atcatttatg
    54601 gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc ttttgccttt
    54661 tttctgtaaa ggttctatcc aactagaaag gtgtcaatat gtttgaaatt ttaatgattt
    54721 tttttattta ccttatttct ctgaatatcg ctgcttttct gggagtcggt attttatccc
    54781 ttttctttca attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt
    54841 ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca cattagttac atggttgcat
    54901 tatgcttatt agcgacaatc aattactatt cttttttcga tcattcaatc gcttatacga
    54961 ttacgctact gattgctggg atttttcact tatcttataa atatcagctg aataaaaacc
    55021 acttgaatcg gactttccgc taaaaaatat ccgtacgata agtagagtgc cttgaagatc
    55081 ggcaaaaaac tgccggactt tttagaggct gtgacaaaga ttttgtcaca gcctcttttt
    55141 ccatataaac ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct tcgttaagaa
    55201 gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag cagctcatcc atggacaagt
    55261 tcatcgtttt ttctttttct tcttccgctg attcagccag tagctgttcc cgatacacct
    55321 gatacatacc agctattttt tgctgcatcg cttttctagt ttcaccttcc gcattgatta
    55381 ttggcaattc tttgatatag ccacgataaa tcaaccgatc atctgaaaat tcaatgggta
    55441 attcaacgag tgtcaacccg acttcctgaa aatattcttc cagcatctat tatcccccca
    55501 ttctttttct taaatgaatt ttagcttacc tactgaaaat tttccagttt cattattttg
    55561 agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt
    55621 tcttctgcca actcctcaat cataagtcac aatattcaac aaacatgaga aactgttttt
    55681 tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc acaacctctt ggtaaacggc
    55741 gttcaatcag ctgaccctcg gtattagctc aaaaaacggt aaaatatgag aagctatttt
    55801 tccgttttgt ttctctaata cttaggataa aacgctgatt ttacaacctc tgttaaacgg
    55861 tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga ggaactattt
    55921 ttccgtttgt ttctctaata ctcaggacaa aacgctgatt ttacaacctc ctgataaacg
    55981 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagaagcta
    56041 tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac ctctgatttc
    56101 ttattcttct tcatttgtag caaaataatt acggtgataa atgatacaac atacactgat
    56161 aagaacaatg gcacctgcaa aataaaacgc atagctttca ccaatatgtt caccaatcac
    56221 acctgcaata aatggtgcta aagctccacc gaaaaagcgg acaaaactat agctggacga
    56281 agcaacgttt cgttgcagac ctggtatttc catagcaatc gtcgtcaaca gtgtattgat
    56341 aattccttgg aaaaatcctg ctaacacaat gctgatcggt acaaggactg gcatagaagt
    56401 tccagctcct aataacagca aacaaataat gaataagaca aatgctgcta aaattgtccg
    56461 ataagtcgta aacttccgct caagagccgg tgcaacgaaa atcgaagcaa tagccagaag
    56521 cacaccccag ccaaaaaata caaaaccgac ttctaattca gaatagccaa tcaataaaaa
    56581 tggcgcatac gcaagtaaag taaagaatcc gaaattatat aacaatgcga ttagtccaac
    56641 tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc caaaggccta cttttctctt
    56701 tggtttctca gtttcaggta aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa
    56761 tgctacccca aaaaatggat aacgccatga accactgccc aagaccccac cgactagagg
    56821 gccaacagcc attccgcagc caagtgctgc ttcatacatc atgattgctt tttctgtgtt
    56881 accaactaaa atactgacga tcgttgctaa agcagtagag acaaataaag cattcccgac
    56941 accccagccg gcacgtaacc caatcagcat tccgatattc ccagataatc cgccaagacc
    57001 agcgaataca acgatgataa acaatcctgt catcaatgtt tttttcaaac cgatccgact
    57061 agataagaaa cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa
    57121 cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc
    57181 tactagaccc actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact
    57241 tttccccttt gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc
    57301 catccctttc tctctattag catcatacat gatttatttt acatgtcaat atgcacatgt
    57361 aaaacgtgga aactttactg tgatataata aagaaaataa gacgagaggt aggattttta
    57421 tgaatacttt agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg
    57481 aattgaagca gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc
    57541 ttgccaagtt agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa
    57601 aaaagatcta ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg
    57661 aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc
    57721 tcgataaaac aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca
    57781 tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac
    57841 gttatgttgt gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc
    57901 aagtaagaga acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa
    57961 tatctattga agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc
    58021 gatccgaacg acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca
    58081 agagactatg acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca
    58141 ttactttgaa ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac
    58201 ggtaaagcgg caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca
    58261 ggtattcctt gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc
    58321 atcaagcatg aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta
    58381 aaggcaattt ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg
    58441 aatccaggca ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa
    58501 ttatacgcgc tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct
    58561 tcttttttat ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta
    58621 gcacatttaa aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac
    58681 ttcatcagcc gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg
    58741 atatatttgc attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg
    58801 ttcatctaac agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt
    58861 cgcttctccc ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa
    58921 gtgattcagc aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg
    58981 aatgatggat tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac
    59041 tttcggagag agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt
    59101 acctgaacca ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc
    59161 atttaccagt aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata
    59221 gcggctgtgg agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg
    59281 cttggttgtt tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat
    59341 ggctttttgt acactgtctt tttgtttaga ggaagacaac cgatccagtc tgatttgttg
    59401 ctttttcttt ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat
    59461 cgatttttcc aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt
    59521 tttctccaat tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc
    59581 tccgtctttt acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact
    59641 aacaaaaacc agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt
    59701 tctatctaga tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag
    59761 acgtatcaac tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc
    59821 cagtcttaac tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata
    59881 ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa
    59941 aagttcaccg gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat
    60001 ccctatacga tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc
    60061 aaagctgact tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct
    60121 atttcatgta aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa
    60181 aaactgcatc acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct
    60241 gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa
    60301 ttgttgttta tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa
    60361 tttcatcgat gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca
    60421 aaaaaagcaa tagctactga atagtaactg aaaacctttt attcgaacat agtgatcttt
    60481 tctggtgcta aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata
    60541 ataactggaa cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg
    60601 aatccaccaa ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata
    60661 gccgctaagc tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg
    60721 taataaggaa tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat
    60781 tcgtaagctt ggttgtagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc
    60841 atgaccattg cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag
    60901 atttcttttg cttcctcgct ttcagcgacc acaaagcgcc atggtttcat attgacagaa
    60961 gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat
    61021 gtttcatcat atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt
    61081 tgttccatta ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa
    61141 tttatctaaa agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac
    61201 ggaacctgta tctttatggt ccatttcaca atgcttgatt ttcttctggg ctttttctgt
    61261 tagcgctaca tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt
    61321 tgcttccaat accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg
    61381 caaactgctt tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt
    61441 aagacttata ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat
    61501 ttgatataaa aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga
    61561 ctcgtcaacg attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc
    61621 tgtgtaataa aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa
    61681 tgaacggaaa aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca
    61741 aactatagaa gatagctctt ctgcagtaga taattttaca ccgccaaaaa tttccgaaat
    61801 cgccttttgc aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat
    61861 aaaaaagtta tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt
    61921 gcatctgcgg taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg
    61981 ttcttactac ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc
    62041 tattccttca tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt
    62101 atagtgatgg gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc
    62161 accagaataa aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc
    62221 agattgtgcc attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg
    62281 ttgtccgctg tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag
    62341 ataatttttt tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag
    62401 acgatacatc ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc
    62461 gattacattg ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca
    62521 agccgtagca tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac
    62581 gcctggctga aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta
    62641 cgaaggatat tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga
    62701 agtaggcatc gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt
    62761 aggggcagaa gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat
    62821 gactgatgca tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc
    62881 aatgaacgct tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt
    62941 ttcgaattca gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg
    63001 gttcgtgatt tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc
    63061 ggtttgtgtt tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga
    63121 gacagtagct gactgcaaag tactttctgt atcgttttca attcctgttt ctgaagaact
    63181 cgatgtctga gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac
    63241 tgctagattt cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct
    63301 cctttgaatt atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca
    63361 atgtccatat ttaaataaca tttgtttctt tttaataaat ttgctttttc tgcataaaaa
    63421 gacgtaaatg aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac
    63481 tatttaacaa gtaattctct ctggatatta tccgttatgg attatacttc ctgtgtcgtc
    63541 ggacggataa tcatgttgct gacagacatc cggtctggcg tttctatcgc aaatgctaca
    63601 gcagaagcta tatcttcaga tgttaatccc cattcttttt gcgcttcatg aagttcaaga
    63661 gcagcctttt tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg
    63721 atcgtagatt tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg
    63781 acagcaaatt ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca
    63841 gaatcagttg aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa
    63901 attccattta acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga
    63961 ccttcaacta atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga
    64021 ccgaactttt ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct
    64081 gctgtttgaa tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct
    64141 tctcggcgtg cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct
    64201 tcaccgattc cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt
    64261 ctattctcct cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt
    64321 actgttcaaa taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg
    64381 gtattgtctg ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca
    64441 atcgttacgc ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg
    64501 attggcttta ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg
    64561 aggttgacac gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg
    64621 aacatcactt gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct
    64681 gtataaaaag gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct
    64741 agccgtttgc ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct
    64801 tcgttttccg cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct
    64861 ttattgacga tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact
    64921 ttataacgga cggcttatga tgtctaatac ttataaaaca tacttaacta ttccttttaa
    64981 ctatacttag tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg
    65041 acgtattttt tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat
    65101 atcacgcaac ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca
    65161 cttttcactc gaggctcccg agagatacag ttgacggatt ctggtcgata tctttattca
    65221 aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca
    65281 atcatctctg gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct
    65341 gatagccttc attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg
    65401 aatgcagatg atatcataga caaactggat agcggattac ttgattttgg actcgtaatc
    65461 gaaccgacaa acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga
    65521 cttttgatac atcggaatca tcctttagca aagcaaacag tcatccatcc gcaggaactg
    65581 aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg
    65641 ctaggtttca gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct
    65701 tctttgatgg tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact
    65761 tatgggacgg aaaaagtatt catcccactc gatccgccat tacaagcttc actgaatctc
    65821 atttggaaaa aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg
    65881 gagaaataca gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag
    65941 ttaagcaaaa gatccaatga ttaaataaca taagtttctt aatttgttcc ttccttttat
    66001 ttctgataat tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt
    66061 ctatataaaa ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca
    66121 tgaaaaatct ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg
    66181 ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga
    66241 aaatcaaaaa gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag
    66301 tagctattgg aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg
    66361 atgtcgaacg gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta
    66421 atcagattga tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg
    66481 ttttgatgga aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt
    66541 ctgcggagaa cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag
    66601 tgctttacag tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc
    66661 ttgggattcc atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac
    66721 gaagcggtcg gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca
    66781 tccatttagt ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt
    66841 attcaagact ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg
    66901 ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga
    66961 tttctaaaaa aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag
    67021 aactgggtta cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg
    67081 ttaacaatca tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct
    67141 tcaacttcca gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg
    67201 ggcatcttat agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt
    67261 aggtcaatta cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc
    67321 aggtgctgct gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac
    67381 tgattcagta gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca
    67441 gatcagtaac gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg
    67501 tttccacgaa gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc
    67561 aatcttggct gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc
    67621 ggtttactct gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc
    67681 aggtctttat ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa
    67741 ctccgtgttc tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga
    67801 aggaacaaaa ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg
    67861 gcttccagca ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac
    67921 agcttccttg atgttagcag cagcttttgc ttcattcttt actggtgtaa cagaaccact
    67981 tgaattctca tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg
    68041 gatttcttta gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg
    68101 atttgtcgat tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat
    68161 cgtccaagga ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa
    68221 attccatctg atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac
    68281 gactaattca gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg
    68341 agccgacaat gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga
    68401 tacaggcctg atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat
    68461 cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat
    68521 ggcaaaacta catggtggtg ctgcaacacg acctgcacaa acaaacaccg tggttaaaac
    68581 agaaacattt gccggtggaa cgatcgaaca agatatttac gctatcgcaa atgggaaact
    68641 gattccaatc actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta
    68701 tgctgtatta ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat
    68761 tttcccaacg aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca
    68821 tatgggcatc aacactgttg acttaaaagg cgaaccattc actttatatg taaaagaagg
    68881 tcaaaaagtc gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc
    68941 aggcaaaaac acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga
    69001 aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc
    69061 ctgagccatc gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa
    69121 tcttgtctca gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat
    69181 aagtccagcc aaacgtaaca tatgaagagc tattttttat ttgactcttc ttattacttg
    69241 atgcagaaca gcttttttac aacctctgat aaacggtgtt caaaaaccag tttatcggta
    69301 tgaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt cttcatactc
    69361 aaaactaatc ggctttttca caacctcttt atacattatt cctgatccaa tatttctttt
    69421 aatgctgctt caagagaagt taatggtttt cccaatactt tttctaaatc atcagaggaa
    69481 atagctaact gatccttttt aatatcatgt tggatcgcta aaaacatgtc agctgcttct
    69541 tgagggaatc cggcttcttt tagattttcg acaaacgctg catcacttgc ttcaataatt
    69601 tctaactttt tacctgtcgc acgctctaat gccttggcta attcttcata gtcagttaga
    69661 ttgccggata attctaagat ttctggagag tcactttcta ctagtacttt tgctgcagct
    69721 tctgcatatt cacgtttcaa tgcccaacct gtttttccat ttcctgcagc ataaacgaat
    69781 tgaccagttt ttaaagcctg cccaatgata ggtaattcat tctctacata ccaattattc
    69841 cgtaaaaatg tatgggaaat acctgaatcc ttaatcattt tttcagtgaa ttggtgatct
    69901 ggcgctaata cactgatcga gtgatcggca tctgcaaaac ttgtataagc gatataagat
    69961 accccagctg cttgtgctgc cttcactaca ttcttatgtt cggcttgacg atttcctgga
    70021 acgccagaga caaaaagcaa tcgatctatt cctttcaacg cttctttcat tgagtctaaa
    70081 tcatcataat caccaatacg tatattaaat ccagcttctt tcagtgcttc tcctttttct
    70141 tcactacgta ctagtacata gatttcattt tgtggaacca tcttttttaa gaattccagc
    70201 gcataaccac caaaacctcc agttgctcct gttactaaat aatgcatatt tgccactccc
    70261 tattcttttt atttgttaag atttacagct gtacatttat aaattacacc tttatcataa
    70321 agagtgtggt aagataagtc aagcaaaaaa aatgaaagga tgacaactga tgaagtattc
    70381 tctgcaattt agtgatgcaa tccatgtact tgcctatatt gaaatgtttc aagatacccg
    70441 atcactttct agtgagatga ttgccagtag tatcgaaaca aatgcagcaa atgtacgtaa
    70501 aatcatgagt cagctcaaaa aatccggttt gatctttaca aaaaccggaa aaccgcagcc
    70561 ttcccttgcc aaaagtccag atcagatttc tttactagat gtctatcgca gcatcgaagg
    70621 taatacacag cttatccaag tcgatccaaa aacaaatcca aattgcgtcg ttggtgcgaa
    70681 tatccaagaa gctttagaag aaagctacgc tcggctgcag aaaaaggcag aagaagaaat
    70741 ggctaagatc acacttgata gtctgataca taaaatagcc gttttggaaa aagaaaaaag
    70801 accagaaaac acaggcctcg tagagagatt tttataattc tctctactgt gcctgcattt
    70861 ttctgatcgg aatcgtctac ataaattctt ttattctccg ggggacaaaa gccttgtcat
    70921 gccccttttt cctctctaat gctcaggtca aagcgctgaa gtcatgacgt cttgataaac
    70981 ggtgttcaag tagctgaccc tcggcattag ttctgaaaaa tgcaaaatat ggaaagctat
    71041 ttcccatttt tcctctctaa tgctcaggtc aaagcgctac tttcacgacc tctatattat
    71101 catggtttat tgactaccgg tatccagatt tctcccatat atttcccgtt ggtaactccc
    71161 atttcgacta cagcatttgg acccccaaca taggcgtaat cttctacttc tcttaaaaca
    71221 tgcccaaagg ccgtattcgt caaatcattt cttaattctt caggcgattc tgctaaagcg
    71281 attacaacaa gatacgttcc ttttgggaat gggatgattc gggtagtgtt tggaacatcc
    71341 tgactcgttt gaacaccgac atagtacatc attttcccgt tatagggttc gttgactgta
    71401 aagaaataat gattttcagc agcttgtttc aatgcatcta ttgttccatt accgataact
    71461 ttttgctcaa atgcttgttt ctctgcagcc aaaccgacaa aatccgtatt gctactcttc
    71521 aattccacac caatacccgt caccgtaaaa tctggttttt cttcgatcct atactttgcc
    71581 atttctccca ccctttctat tatattaagt tcatcttaca taaaattaaa gggaaatgac
    71641 acgattacgc tcttttttct agggatattt ttatttttta actatttttt tgctttcact
    71701 gcaatccaaa tctcactgta aacattactg aaatcctctc cccattttgt aaaagagatc
    71761 tcgggagctt cgactaactc ataatctgaa gaaggaagcc attctgcata gattttcccc
    71821 caagtatctt gtaaagtttg gggaaatggc cctttattag gaaaaatggc ccaagtgtgt
    71881 tcttctatag caagcatttc taaatcagcg tacggatttt cttttgtcgt agcaaagcca
    71941 atcatgtgca tcaaactgcc cttttcttcc aagcgggctc catcaaatcc ataagaaacg
    72001 ttgattactt gatttggata taaatctcct aacttatgca tctcttccct ttgagtctcc
    72061 gtgatcgtct gcgctaactt ttgtatttct tgattgactc cttcaaattt gacaggaact
    72121 tttttcgcca cacctgccac ataaaactgt tctttctttt cgatacgaaa ttccatcgat
    72181 tttcctcctc tgacatcaat aaaaaatgtc aagcgtggaa aagatttttg tatgccgtat
    72241 ctgctgattt ctgaaggcag atatcctgac caatcacgaa aagctcttga aaaaccttcg
    72301 actgtttcat atccatatct aaacgccgtt tctgtcactc ttgatccatc taggagatcg
    72361 cgattagcca aagccaatct acggtttttg atatagtcca ttagcgacat ccctgcaata
    72421 aacgtaaaga tgcgttttaa ttgatattca gagattcctg tattttcggc tattatttta
    72481 taggaaagtc cttctgtcaa atgttcttcg atgtaatcga tcaattggtt cagatcttta
    72541 agcatattta tcttcctcct tgcttgcctt tttatcatat tatcaaagaa agtcaattac
    72601 ttgatgaatc aggaaccaat ttagtcagat gtagaaaatg ttgtatggat ctaaagaaaa
    72661 ttctcaagca gcgtttcgta acggataatt ggtattatct gcctatctat gttttacctt
    72721 tagaaaaata aattttgggg aagacacatt ctaagtaaac aaagcggtaa aaatatcttt
    72781 tattccctta gttaatccgc ttactaccta ctgtacaaag caaattgttg ttcaaactgc
    72841 tgatcagaac gctgcttgga tttttctttg cgatacatca tacctctttt atcaattgga
    72901 acaattattt atttctaaca aaatcacttc atttttgtca gataaataaa aaacaaaaaa
    72961 agttccatct ttaaaacacg ctattctttt actttttctc acattctcta agaaaagaaa
    73021 aggagaatga atttttgaca attcgagcaa aagtgattat caaatatgta aaatcagcta
    73081 cttttttctt aaacgtttct ttatactaaa tataaataag tgtttcatga aaggagaact
    73141 agatgaataa ttggcttgag tattttcctg aaaacgtttt ggaacgcggt tacagctatc
    73201 atctgcacgg ttttgtccgt catttgaact atacaagcaa gtacttgtcc gcaacagttt
    73261 ctggcacgga agattataag gtagtgatca cttgggatga aaagaccaac atgacttgtg
    73321 attgtctata tgccatagaa ggaaaaaaat gcaaacacat ggctgctgtc ttattcgctt
    73381 acgaagaacg tccaataaaa aaatccaact actcactttc cgaactctcg tcccttgttt
    73441 cctccgctag ttcctctctt gtacgggagc ttttaacaga gattcttata gaacatccgc
    73501 aatttatcga acgcttcaaa gttaagatgc catttcatgc aataaattat tcagataaat
    73561 taacaactat catccataaa tacgatcata ttatcaagaa aaacaaaaac aggaaaacag
    73621 cgaagttcat catggagatg cgaaagttca tccaagaagc agttgaatcg ctcattcagc
    73681 aaaatgccta tcttcccgca ttcgaattga tcaatgaagt gattgctact ctcgaaacat
    73741 tctattggga gcctgaggat gaacgaaccc ttcttctgat agaggactgt tattatttat
    73801 ggaaagaatt attagcagaa gcacctcatg cagaaaaaag gcaaatgttc tcttggttcg
    73861 tctgccaagt cgatcatact gacgcctctt attcaaaacg ctactccata aaaatcctga
    73921 aagaagactt tcgggaaaaa gaattttcta accaaaagaa aaaaatagat aaaaaaacta
    73981 aagaaacggt aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc
    74041 ctaaatccac aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga
    74101 agtaaagaag acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg
    74161 ttttataagt catgacaaat ttccttcgtt gctgggtaga ttttccggca ttgttgatag
    74221 attttatgat acttttctac attttgtaag agaggttcca ctgcatctgt atattggata
    74281 aaattctgac aacaactttc aggtgtgtcg aaccactcta atctttaaag ataaatatta
    74341 aaataacgat taataatatt aacagtcttt atttttcacc accaaaaacc tatctttgca
    74401 tatactaaat agacgtatat acacagatag gtttgtcttg tgaaaaataa tgattatcct
    74461 taccaaaaaa tgtttgtttt tctacatgtt cgtacttatc aaaaaatatg atccttattg
    74521 aaattactgg ctatttatat tccaacgact tcttgtgtta atttttgtta tctcatagta
    74581 aattatgttt cattttcact taatttagtt gtcacgtgtt cgtcaattcg taaaaacggt
    74641 aaatatataa acacacttgc ggctataatt aatatttgta aaacagctgc tcgccagtct
    74701 ccggctgttg ctaaaaatcc cgatataact ggtggcgtag tccaaggaat ttgcactact
    74761 acatgattaa ttaatcccac agcagttgca aaatatgcta ctgtcaaaga aaaaatcggt
    74821 gcaagcacaa agggaataat cagactgata ttaaatacaa taggcaatcc aaaaataata
    74881 ggttcgctta tattgaatat agcgggagtt accgataatt tagcaaagtc cttataatct
    74941 tttctacgac tgaagataaa tattgcaatc aataaagcaa tcgtatttcc cgatccaccc
    75001 attaccgcaa aggtatcttt aaaggccata ttgataatat gtggaatttg ctctttgttt
    75061 gcataagctg ccatattttc ttgcatattc tgaatcaaaa acggatctaa caatgaacct
    75121 gatattactg cttgatggat accaaatcca aaaaatagat tagcaataga tgtaattaac
    75181 aaaaatcctg gtaaaccagt tgtaatttta cttaatggct ctgta
//
LOCUS       DAYYFD010000003        65563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000003 DAYYFD010000000
VERSION     DAYYFD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.003"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..839
                     /locus_tag="ACHA25_000163"
     CDS             207..839
                     /locus_tag="ACHA25_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8761501.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            962..1600
                     /locus_tag="ACHA25_000164"
     CDS             962..1600
                     /locus_tag="ACHA25_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8761502.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(1719..1791)
                     /locus_tag="ACHA25_000165"
     tRNA            complement(1719..1791)
                     /locus_tag="ACHA25_000165"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1756..1758),aa:Thr,seq:cgt)
     gene            1912..2154
                     /locus_tag="ACHA25_000166"
     CDS             1912..2154
                     /locus_tag="ACHA25_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761503.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2293..2922)
                     /gene="upp"
                     /locus_tag="ACHA25_000167"
     CDS             complement(2293..2922)
                     /gene="upp"
                     /locus_tag="ACHA25_000167"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8761504.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3056..4003)
                     /locus_tag="ACHA25_000168"
     CDS             complement(3056..4003)
                     /locus_tag="ACHA25_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8761505.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4156..5400)
                     /gene="glyA"
                     /locus_tag="ACHA25_000169"
     CDS             complement(4156..5400)
                     /gene="glyA"
                     /locus_tag="ACHA25_000169"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8761506.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(5591..6616)
                     /locus_tag="ACHA25_000170"
     CDS             complement(5591..6616)
                     /locus_tag="ACHA25_000170"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8761507.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(6638..6817)
                     /locus_tag="ACHA25_000171"
     CDS             complement(6638..6817)
                     /locus_tag="ACHA25_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761508.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(6810..7649)
                     /gene="prmC"
                     /locus_tag="ACHA25_000172"
     CDS             complement(6810..7649)
                     /gene="prmC"
                     /locus_tag="ACHA25_000172"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8761509.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(7642..8715)
                     /gene="prfA"
                     /locus_tag="ACHA25_000173"
     CDS             complement(7642..8715)
                     /gene="prfA"
                     /locus_tag="ACHA25_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8761510.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(8718..9302)
                     /locus_tag="ACHA25_000174"
     CDS             complement(8718..9302)
                     /locus_tag="ACHA25_000174"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8761511.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9408..9656)
                     /locus_tag="ACHA25_000175"
     CDS             complement(9408..9656)
                     /locus_tag="ACHA25_000175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8761512.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            complement(9647..9877)
                     /locus_tag="ACHA25_000176"
     CDS             complement(9647..9877)
                     /locus_tag="ACHA25_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761513.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            9983..10585
                     /locus_tag="ACHA25_000177"
     CDS             9983..10585
                     /locus_tag="ACHA25_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8761514.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(10689..11258)
                     /gene="lepB"
                     /locus_tag="ACHA25_000178"
     CDS             complement(10689..11258)
                     /gene="lepB"
                     /locus_tag="ACHA25_000178"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8761515.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(11488..12255)
                     /locus_tag="ACHA25_000179"
     CDS             complement(11488..12255)
                     /locus_tag="ACHA25_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8761516.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12252..12731)
                     /locus_tag="ACHA25_000180"
     CDS             complement(12252..12731)
                     /locus_tag="ACHA25_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8761517.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(12856..13593)
                     /locus_tag="ACHA25_000181"
     CDS             complement(12856..13593)
                     /locus_tag="ACHA25_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8761518.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            13754..15154
                     /locus_tag="ACHA25_000182"
     CDS             13754..15154
                     /locus_tag="ACHA25_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8761519.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15377..16729
                     /locus_tag="ACHA25_000183"
     CDS             15377..16729
                     /locus_tag="ACHA25_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8761520.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            16722..17420
                     /locus_tag="ACHA25_000184"
     CDS             16722..17420
                     /locus_tag="ACHA25_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8761521.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(17482..18633)
                     /locus_tag="ACHA25_000185"
     CDS             complement(17482..18633)
                     /locus_tag="ACHA25_000185"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8761522.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(18646..19083)
                     /locus_tag="ACHA25_000186"
     CDS             complement(18646..19083)
                     /locus_tag="ACHA25_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8761523.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19098..20864)
                     /locus_tag="ACHA25_000187"
     CDS             complement(19098..20864)
                     /locus_tag="ACHA25_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8761524.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21128..21805
                     /locus_tag="ACHA25_000188"
     CDS             21128..21805
                     /locus_tag="ACHA25_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8761525.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            21808..22935
                     /locus_tag="ACHA25_000189"
     CDS             21808..22935
                     /locus_tag="ACHA25_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8761526.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23135..23575)
                     /locus_tag="ACHA25_000190"
     CDS             complement(23135..23575)
                     /locus_tag="ACHA25_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8761527.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(23586..25619)
                     /locus_tag="ACHA25_000191"
     CDS             complement(23586..25619)
                     /locus_tag="ACHA25_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8761528.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(25621..27324)
                     /locus_tag="ACHA25_000192"
     CDS             complement(25621..27324)
                     /locus_tag="ACHA25_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8761529.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27418..28368)
                     /gene="manA"
                     /locus_tag="ACHA25_000193"
     CDS             complement(27418..28368)
                     /gene="manA"
                     /locus_tag="ACHA25_000193"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8761530.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28407..29432)
                     /locus_tag="ACHA25_000194"
     CDS             complement(28407..29432)
                     /locus_tag="ACHA25_000194"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8761531.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(29641..30510)
                     /locus_tag="ACHA25_000195"
     CDS             complement(29641..30510)
                     /locus_tag="ACHA25_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8761532.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31214..33106)
                     /locus_tag="ACHA25_000196"
     CDS             complement(31214..33106)
                     /locus_tag="ACHA25_000196"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8761533.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33103..34830)
                     /locus_tag="ACHA25_000197"
     CDS             complement(33103..34830)
                     /locus_tag="ACHA25_000197"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8761534.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(34846..35496)
                     /locus_tag="ACHA25_000198"
     CDS             complement(34846..35496)
                     /locus_tag="ACHA25_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8761535.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            35700..35996
                     /locus_tag="ACHA25_000199"
     CDS             35700..35996
                     /locus_tag="ACHA25_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8761536.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36188..36808
                     /locus_tag="ACHA25_000200"
     CDS             36188..36808
                     /locus_tag="ACHA25_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8761537.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(36900..38066)
                     /locus_tag="ACHA25_000201"
     CDS             complement(36900..38066)
                     /locus_tag="ACHA25_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8761538.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38078..38431)
                     /locus_tag="ACHA25_000202"
     CDS             complement(38078..38431)
                     /locus_tag="ACHA25_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8761539.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            38559..39581
                     /locus_tag="ACHA25_000203"
     CDS             38559..39581
                     /locus_tag="ACHA25_000203"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8761540.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            39600..39761
                     /locus_tag="ACHA25_000204"
     CDS             39600..39761
                     /locus_tag="ACHA25_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761541.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(40020..40454)
                     /locus_tag="ACHA25_000205"
     CDS             complement(40020..40454)
                     /locus_tag="ACHA25_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761542.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(40564..42423)
                     /locus_tag="ACHA25_000206"
     CDS             complement(40564..42423)
                     /locus_tag="ACHA25_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8761543.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            42424..42564
                     /locus_tag="ACHA25_000207"
     CDS             42424..42564
                     /locus_tag="ACHA25_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8761544.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(42541..43701)
                     /locus_tag="ACHA25_000208"
     CDS             complement(42541..43701)
                     /locus_tag="ACHA25_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8761545.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(44008..45432)
                     /gene="araA"
                     /locus_tag="ACHA25_000209"
     CDS             complement(44008..45432)
                     /gene="araA"
                     /locus_tag="ACHA25_000209"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8761546.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45448..46146)
                     /locus_tag="ACHA25_000210"
     CDS             complement(45448..46146)
                     /locus_tag="ACHA25_000210"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8761547.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46103..47764)
                     /locus_tag="ACHA25_000211"
     CDS             complement(46103..47764)
                     /locus_tag="ACHA25_000211"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8761548.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            47982..49382
                     /locus_tag="ACHA25_000212"
     CDS             47982..49382
                     /locus_tag="ACHA25_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8761549.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(49634..50713)
                     /locus_tag="ACHA25_000213"
     CDS             complement(49634..50713)
                     /locus_tag="ACHA25_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8761550.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(50799..51758)
                     /locus_tag="ACHA25_000214"
     CDS             complement(50799..51758)
                     /locus_tag="ACHA25_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8761551.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(51770..53281)
                     /locus_tag="ACHA25_000215"
     CDS             complement(51770..53281)
                     /locus_tag="ACHA25_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8761552.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53303..53866)
                     /locus_tag="ACHA25_000216"
     CDS             complement(53303..53866)
                     /locus_tag="ACHA25_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8761553.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(53872..54735)
                     /locus_tag="ACHA25_000217"
     CDS             complement(53872..54735)
                     /locus_tag="ACHA25_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8761554.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(54749..55645)
                     /locus_tag="ACHA25_000218"
     CDS             complement(54749..55645)
                     /locus_tag="ACHA25_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8761555.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(55820..57109)
                     /locus_tag="ACHA25_000219"
     CDS             complement(55820..57109)
                     /locus_tag="ACHA25_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8761556.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57081..58598)
                     /locus_tag="ACHA25_000220"
     CDS             complement(57081..58598)
                     /locus_tag="ACHA25_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8761557.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            58796..59686
                     /locus_tag="ACHA25_000221"
     CDS             58796..59686
                     /locus_tag="ACHA25_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8761558.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(59759..60583)
                     /gene="nadE"
                     /locus_tag="ACHA25_000222"
     CDS             complement(59759..60583)
                     /gene="nadE"
                     /locus_tag="ACHA25_000222"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8761559.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(60595..62067)
                     /locus_tag="ACHA25_000223"
     CDS             complement(60595..62067)
                     /locus_tag="ACHA25_000223"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8761560.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(62704..63066)
                     /locus_tag="ACHA25_000224"
     CDS             complement(62704..63066)
                     /locus_tag="ACHA25_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8761561.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63289..64041
                     /locus_tag="ACHA25_000225"
     CDS             63289..64041
                     /locus_tag="ACHA25_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8761562.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64181..65104)
                     /locus_tag="ACHA25_000226"
     CDS             complement(64181..65104)
                     /locus_tag="ACHA25_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8761563.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65119..65391)
                     /locus_tag="ACHA25_000227"
     CDS             complement(65119..65391)
                     /locus_tag="ACHA25_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761564.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18956 a        13434 c        11413 g        21760 t
ORIGIN      
        1 tctaaattaa atcagtaaaa atttttattc taagattttt attagaatta cgcctaattt
       61 gtgtctctca gggcaattct aagaacgttt caagagatag cgacactatc gttatcgaaa
      121 aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg tttagctttt gttaatatga
      181 ctttatgaaa atggaggtaa ttaaatatga tttttcctaa agttaatgaa gcaagcttga
      241 ataaaggatc cttttttcat atctatgaaa acgaagattt tgaactgtat aatatccttt
      301 cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc aattatttac aaatctattt
      361 tcaagtatgc attttttgct tccaatgatt ttcaaagaat ggagattacc gactgtatat
      421 tcgaaaactg tgatttttca ggctgtatat tgacaaaaag catgttgcat cgcgtgaaat
      481 ttgtcaattg taaattaaca ggtactaaat tgatggaaag ttatattggg aacacgttat
      541 tcgagaattg taaaatggac tatgtaaatt tatctggttc aaacataaaa gaaagtacct
      601 ttgagcactc tgtcttatca agcgcagatt ttgtagactg ttccctcact aaaataatgt
      661 tttctactaa tgatttagaa aatgctagct tttttgatac tgatttaaaa aatatcgatt
      721 taagtagcaa ctcctttgaa atgattgaag tacgctcgga acatctaaaa aattgtacgc
      781 ttaatcagtt gcaagcactt ggttttgcta gaaaattttt acaaataaaa gtagtataat
      841 caaaatgaat atataagata aatttttagc aatacaaata tccataaaaa aacattgcct
      901 attttaactc ttcatgtata ctgactctat cttcaaaaac tgaatagtag gagttattat
      961 catgaataac attttaggaa aaaaaataga acttaaaaaa gggatattgg gcactattgt
     1021 cgttgtactc ggtttgttag ggttgctatt tgcgccatcc attattagca ttttttcttt
     1081 aactatcact ggatttatag catttacact tgtttttggt aaaaaagata cgaaaaaaat
     1141 gttctcgaaa cctgtcgctc ctgtcaaaaa tatagccaaa tattttttta tcaatgtagt
     1201 aatcagtgcc gcagtctcgg tatttttgca acaaattttg aaatggggtt taacaggcaa
     1261 tccaataaat gaggctttca gcccgttatt atttattatt ttgcctatca tgatacttgg
     1321 agaagaattg ttctcagtgt attttctcgc tattttttct agtaagttca gcattcctgt
     1381 cgctagtttc ttgtcagcaa tcatctttgg atttatccat tacagtactt atgataatgg
     1441 aaatatttta cacactgtag cacacatcct tttgatacaa ggggtggcaa gattgttatt
     1501 caaccaagct gccattaaaa gcaactcgat cattacaagt tgggcagttc atgtgatatt
     1561 tgatttcaca gctatattgc tagtcgtttt attctcctaa atttttatag aaataataca
     1621 tgctctattg ctaagaaagc atactttatt tcctttaaac aaaaagacag atgtatcaat
     1681 ggattaaaaa tccttgatat atctgtcttc tcttttaatg ccagctatag ggatcgaacc
     1741 tacgacctac gcgttacgag tgcgttgctc taccaactga gctaagctgg ccttatccaa
     1801 aagaaattat atactctatt tggctgattg taaagagtcc gatggaaaat tagtttaatt
     1861 tcatgatatt tttcataaaa cgtggtataa ttgatgtgaa aggcaggttt tatgaaaatg
     1921 gcaacttttg gaaaatttga tacaactatc gtaccatcgg aaattggtga aaaattcaag
     1981 atcaatactc acgtaatgtt cacaattcac ggcaagtcgc aaactggaac gatttcaaag
     2041 caattgaaaa attccgctgt tgttgagatt gatgaaactg cagaaaatgc gaacttgatg
     2101 acgcaaagta agggtgtagt aatcgttaac tacaaagaac taaaaaagat ttgaatttga
     2161 ccatctctgc atttttggga tggttttttt atatgcaaac gaaagctgtg aaaaagctgt
     2221 tctgcatcaa acgttaaaaa tgaccaaaaa aggatgtgac aaaagttgtg tcacaccctt
     2281 tttcgtattt acttattttg taccaaacaa acggtcaccc gcatccccta gtccaggaac
     2341 gatataacca tgttcattca atttttcatc tagagaagct gtatagatgt caatatcagg
     2401 atgcgcttct tgcaaagctt ttactccctc aggtgctgct accaaacaaa caaatttaat
     2461 gttcgacgcg ccacgttctt tcaatgcatc gattgccatg attgctgatc cgcctgttgc
     2521 caacattgga tctactacaa acaactgacg tgcatcaatg tcttctggaa gtttgacgaa
     2581 atattcatgt ggttccaatg tttcttcatc tcgataaaga ccgatatgtc ccactttagc
     2641 agctggaatc aattccaaga taccgtcaac cattccaata cctgcgcgca agattggaat
     2701 aatcgctact tttttaccag acaacgtttt ttgtgttgtt tcagtgatcg gtgtttcgat
     2761 cacgatgtct tccaatggca tatcccgtga tacttcatat gccatcaaca tagcgatttc
     2821 atcgactact tcacgaaaaa cctttgttcc gcagtttttg tcccgaataa tagttagttt
     2881 gtgttgaatc aatggatgat cgatcacttg aaatttgccc attttttaag ctctcctttg
     2941 ttctgtcgtt tctttttaat tgtaaaacaa aatcaagtga ttgactagtc aaactcgcca
     3001 tttttcctaa gaaaatccgt ttttgaaaag aagttttcct gcggacaagc aaagcttatt
     3061 cttttttctt gatccaagaa gtagataatg ccattgctgc acgcgttgcc tttgtctgat
     3121 ccatagcatt taccatgaca aaatcatgga tgattccttg gaagcgtact tgtgtaacag
     3181 gaacccctgc atcacgaagt ttgcgcgcat aagcctcacc ttcatctctt aatacatccg
     3241 cttctcccgt taaaatcatt gctgaaggaa gatcatttag ttcatttata tttgctctca
     3301 aaggcgaagc tgtgatttca gcacgctctt cttcattcgt cgtatattgg tcccaaaacc
     3361 acatcattcc ttctcttgtc aaaaaatagt tttctccgaa ttcatgatag gaatctgtat
     3421 caaaatcagc gtttgtgact ggataataca gcaattgctt ggatattggg aggccctttc
     3481 gttcttttgt taagatcgtc atgactgtag ccatgttacc acctactgaa tctcctgcaa
     3541 cgattagatt ctcgagatcc agattttttg acttggctaa tttattcagc tgttgcaaca
     3601 cagcataatt ttgttcaatt gctgttggat attttgcttc tggtgatcga ctatattctg
     3661 gaaagacaac gacggaattc gtacgaaccg caagttctct gatcaattta tcatgggttt
     3721 gagcactgcc aaaaacccag cctgctccat gaatataaaa aataactggt aatttatcca
     3781 tattcccttc tggtcgtacg agacgaatat tgagttttcc ccattcgcca gtatccacca
     3841 tcaagtcttc aatatcgacg gaatatttgt tcacaggtga ttcttgtact tcttctagta
     3901 aaactcttcc ttcttccggt gctagttcat agatacgcgg atgaggcgca tttgcatcac
     3961 tgaatgctac tgcttccttt tctaacgaga tacgatcagt catgactttt cctccctgag
     4021 atttagtgtg aagactaaaa ttacacctca tatactagag agcatcaccg atattgtcaa
     4081 agaatccgtt ttcccaaaaa ataaacagaa aagcccaaca ttgaactttt ctgtttacta
     4141 atggaattta aatttttatt ttttatgcaa cggaaatttt tcagttaatt cgcgtacacc
     4201 tgttttgact tcgtctagtt gagcgttatc atcttttgct tgtaatgctt tgacaactag
     4261 ttcagccact tttacagcat cttcttcttt gaacccgcga gtagtgattg ctggcgtgcc
     4321 gattcgaata ccgcttgttt tgaatggact tagtgtttca aaaggaatcg agtttttatt
     4381 tacagtgata ttcacactgt ccaaaatgct ctctgcttct tttccattta tacctaactc
     4441 gcgaacatcg atcaacatca gatgattatc tgtcgcccct gaaatgactc tggttccaat
     4501 tgcttgattg aaaactttga ccattgcttt tgcattggcg atgatttgtt cactatattc
     4561 tttaaatgca ggatctaatg cttctttgaa tgctactgct ttacccgcaa tcacatgctc
     4621 tagagggccg ccttgaatac caggaaatac cgcactattt attttttttg ctaatgcttc
     4681 atcgtttgtc aaaatcatgc cgcctcgagg gccacgcaat gttttatgag tggtagtcgt
     4741 tgtgatatct gcataaggaa ctggattagg atgaagtcct gctgctacta atcctgcgat
     4801 atgtgccata tctaccatca gttttgcgcc tacttcatct gcgatttctc tgaatttcgc
     4861 aaagtcgatc gttcgtccgt aagcactggc acctgctacg atcaattttg gttgatgttt
     4921 tctagctaaa atacgtacca cattataatc aataacttct gttgtcggat caacaccgta
     4981 agcaacaaag tgataggttt tgccactgaa gttgactggt gatccatgtg ttagatgtcc
     5041 gcctgcggaa agatccattc ctaagatcgt gtcccctggt tctaccaaag cgagataagc
     5101 agccgtgttg gcttgagagc cggagtgagg ctggacgttc gcaaatttcg ctccgaataa
     5161 ttcttttgca cggtcaatcg ctaaattttc aacgatatcg acaaattcac acccaccata
     5221 atagcgatgc cctggataac cttctgcgta tttatttgtc aagatacttc cttgtgcagc
     5281 catcactgct tcggagacaa agttttcaga ggcaatcagc tccaaattat gttcttgtct
     5341 ttcttcttct ttggcgatcg cagcccaaag atcagggtca aacgttttgt aatctaccac
     5401 aaacaacagc tcctttttat tttcctacaa ttttattttc ctacaatttt atttttccca
     5461 tcaaaaacaa attgccaaat attcgtatct cttctattac gattagttac gcttagtttt
     5521 tcgttcactg atagaagatt tacttgatag cattgtatca tactaaaaga atgcgacaat
     5581 aaccaattga tcatttttca aaataatttt gtcctgctgc ttttttcaag cgattcatgt
     5641 aagcattccc caatccagtt tctggataca cttgtacaaa aatcaaatct aatccaagtg
     5701 ttggttcatc taatcctctt agacctgcaa acaatccttt agccgccgct tctactgagt
     5761 tgtcgttata catataaaca gctgcagtat ctgtccgcac ttgatccgca atttcggggc
     5821 ttgctatgac acctgcacgg atcttcttat tttttgccca ttgaactgca gttgaccaat
     5881 cgccatctct gaccatcaga acacgtgtat cgggtgagta gtgtttgtat ttcatcccag
     5941 gggcttttgg tgtttcattt tcttttacaa gatgctgatc aatagcaaca ggagattcga
     6001 tgactgcttc gatttgttct ttcgttactg cacctggtcg taaaatcata ggcatggctg
     6061 ttggatcact taaatcaaga acagtggatt ctacaccaat tcgcgtagct ccatcatcaa
     6121 taatcccggt aatttttcct tgtagatcat gatatacatg atctgcagtc gttggacttg
     6181 gttttcccga cgtatttgca cttggaccga caagcggaac accggaaagt tcgattactt
     6241 ctagtgtttt tttgttatcc ggtatacgaa atgcagctgt ggataatcct cccgtcacaa
     6301 ctgagggcaa agtgtctttt ttgatcttaa atatcaaagt taatggtcca ggccagaagt
     6361 tcttcacaat cttctctgct gccggatgaa agttgtccac atactctttt acttgttcaa
     6421 aagaggcgac atggacgatt aatggattat cttgcggtct cccttttaca gaaaacactt
     6481 tcttgacagc gtttggtaaa agagcgttgg cgcccaatcc gtatactgtt tcggtaggaa
     6541 aagcaacaag ttctccttct ttcagtgctt cggctgcctc ttgcaattct gtttcatcat
     6601 atcgtttcgt ttccacagtt tatccacaac ccctttttca tacattctta ttttgtctga
     6661 gtttatcctc aagttttcca ctgtttgtgg ataatctatt tgtcaatagt ggataaactt
     6721 gttgataaac caaaaagtcg tttacatcct tgtcaaataa gtgttttttt ataataaaaa
     6781 aacaaataaa aaatttttca tccacaactt caaccataaa cttggatcat tcgttcattg
     6841 ccaaaaagat ccttttttat cgccactttt ttatctggaa atgcttgctg aaatattttt
     6901 tttactgctt caccttggcg aaatccgatt tctaaaaaaa tctttccatt tgattttagt
     6961 acgcttggtg cttcaactgc tattttttca taaatagcta atccatcgtt ctcagcaaaa
     7021 agggccattt taggttcgaa tgaccggaca ctttcatcca tcagttccca ttcattccga
     7081 ctgatgtacg gcggattaga aataatcaca tcaaattgct gatccattac tggtcctaat
     7141 gtatctccat gataaaaact gattttcgtt ccttctttct gagcattttc tttcgctact
     7201 tctaatgctt cttctgaaag atccacagca gatatatgcc aattttttct tgctgctttc
     7261 aaactaatag caattgctcc agtccctgtc ccaatatcta tcacttctaa cggtacacca
     7321 ggcgtctcgt ctaaacacca ttcgactaac tcctctgttt ctggtcgagg gatcagtgtt
     7381 gcttcagtta cctttaaccg atgatcataa aaatctgcat atcctagcaa atactgaggt
     7441 ggatgatctg caagcagaag tcgcagatcg gtttcgatca accgctgttc ttcttctgaa
     7501 atctcttcgt tcatatgaag gagccaatca agttttttcc attgttttcg ttccaaaaat
     7561 aaaaattgga tactatatgc ttctttccca tgatcctcta aaaaagaaga agcccgtgac
     7621 aggacttctc gataggtcat tttacccatt ttgcatagct tccagttttg atgtttgatc
     7681 atacatgatc aatgcatcaa cgatctcatc taatttccct gctaaaatct gatctaattt
     7741 ttggatagtt aatccgatac gatgatcagt cacacgattt tgcgggaaat tatacgtccg
     7801 gatccgttct gaacgatccc cagttccgac tgctgatttt cgattggcat catactcgct
     7861 ttgtgcttct tgctggatct tatcgtacac acgagcacgc aagattttca ttgctttttc
     7921 acggttcttg atttgagaac gttcatcttg catagcaaca acgatccctg ttggtaagtg
     7981 agtcaaacgt acagcagatg ctgttttatt gacgtgctgt ccgccagcac ctgaagcatg
     8041 atagatatct gtacggatat ctttgtcttc aatatcgatc tctacttctt cagcttctgg
     8101 catcacgaca actgttgcgg tggaagtatg gatacgccct tgcgattcag ttgaaggaac
     8161 acgctggacg cggtgcgcac cactttcata ttttaatttc gaatatacgt tgtctccggt
     8221 gatcatcatg atcacttctt tatatccacc gattccggta acgttagctt ccatcacttc
     8281 tgttttccag ccttgtgctt cagcatactt ttggtacata ttgaaaagat cgccagcgaa
     8341 taatgctgct tcgtctccgc ctgctgctcc acggatttcc atgatgatat ttttatcgtc
     8401 atttggatct tttggtaaaa gcaagatctt gattttttct tctaagactt ctttttcttt
     8461 ttttaattca gataattctt cttttgctag ttcagccatt tcatcatcca gtttttcacc
     8521 aagcagttct tctgtatcgg tgattccttg tactacttct ttataacgac gataaacttc
     8581 gactgtttcg cgcgtgtttg cttcttcttt tgaaagctgc ataaaacgct ttgtatcaga
     8641 aatcacttct gggtcactta aaagttctcc aagctcttca taacggtctt caattgcttg
     8701 taattgatcg tacatgctta ttctccttct gtaatcattg atgggtgaaa gtaatgtttt
     8761 cgacaaacgg ggtaataagc ttcatttccg ccaatttgca cttgatcgcc ttcataaaca
     8821 gggtttccat caatataatg taaattcatg attgcttttt tgtggcagaa ccaacagatt
     8881 gttttcattt cttcgatttt gtctgcataa agcaataaat attttgatcc ttcaaataac
     8941 tcgtttcgaa aatcattttt caaaccaaat gccataaccg gaatatccaa ttcgtccaca
     9001 attctcgtaa aagcaatgac atgtttcttt tttaagaatt gcgcttcatc gatcagcaca
     9061 caatagggtt tgctttccat tttctgaatt acgtcaaata cgtctgtttc ttcaaatatc
     9121 ggcatcgcat tacgtttcag tccgattcgg cttgaaacaa cgcctaaacc atctcttgta
     9181 tcaataccgc ttgtcattaa gacaactggc ttattttgtt cttcataatt atgtgcgact
     9241 tttaggatct cgatcgtctt gccgctattc attgcgccat acttaaaaaa tagttgtgcc
     9301 atgctctttt tcaccttcca gtttgttcct atggtattat acttgatttt cagcattttt
     9361 tacagtgaga attttcatca agcacagatt agtattaaat ctattgctca cttttgttcc
     9421 aattttttga caagaactaa acatgggttc cattcatccc ataaatttgg aaaaacttcc
     9481 aaccttgaaa atcctaatga ttcgtagaaa caaatagttt gatcatatat agagtagtgc
     9541 ccctcgtcta cagtcttcac ttgcaaatat ttgtaatgtt ttgctgcgta accttctgct
     9601 tctttaaaaa gttttgttcc taatccttgg tgatgatagt ctttttttat tcccatacaa
     9661 tagaatagat ttcggcagta tcttcacttg tctgtgaaag agtgataaag cctaaatatt
     9721 cttcttttct tttttcacac cataaaggta gtttacttga ttcctcgata tatttttccg
     9781 ttgattcagg aagcccaaac cactcaggca agtctagtaa tatttctcgg acacactttc
     9841 ctttttcctc tctatctttt acaaacgata tcggcatatt ctttctagat tctccctttt
     9901 tgaattgagt atagcatgct ttctttcttt tcacgtgcta taataagtta aaatttcaaa
     9961 gttttacgta aggagctatt tgatgacaaa aaatattttt gattccatcg ctaaccatta
    10021 tgattcccct gaccgtcaag cattagccaa gattattcgc gaagaactaa ctacttatct
    10081 accgcgcgac agccaccaaa aagtcctgct ggattacgga ggcggaactg ggcttgttag
    10141 tttaccgctt gcggaaagat tcaaagaact gatcattgcc gatgcttcag aaactatgct
    10201 gaaaatggca gaagaaaaga tccaagcagc agatctgaaa aatgtccgaa cgatccacgc
    10261 tgatgcttct gtcgaatttc ctgctgtcca agctaatctc atccttctat cacttgtact
    10321 tttacacatt cctgatacgg agaatattct tacaaaactt tatgaaattt tagctcctgg
    10381 cggtcaattg atcatcgtag actttgacaa gaatgaacaa attagtcatc cgaaagtcca
    10441 taatggcttt agccaagaag aattgaataa tcgattggaa aaaacgggct ttgtctctgc
    10501 tactagtcat accttccatc gtggggaaaa gctatttatg aacaaaaacg cttcgctttt
    10561 cttatccatc agccaaaaag cttaaaatga gattgcaaaa aaagtgagca actccaagaa
    10621 ataagaagga attttcggaa aaagaagctg tgacaaaaaa catgtcacag cttcttttta
    10681 ttttttcatc acatgatttt aatttctgaa agaggccaat aacggaaaac aacttttcct
    10741 tggatcatat ctttgtcgac taacccaaac attcggctgt cttttgaaat caagcggtta
    10801 tcgcctagaa caagatactt tccttttggc acagtatatt caaagtcact cgtaaaggtg
    10861 gatgaacttt ctgcttgtgc ttggaatcct gagctataag catattctcc ttgtaactta
    10921 tcttctgaaa attctttttt gaactcatcc agataaggtt catcatactt tttgccatta
    10981 atggtcaact ggtcattttc catcttgact gtatctccag gcataccgat gatccgttta
    11041 acaatcatcc gttcttcatc ccccggttcc tttgtagtaa taatatccat tcgttcatag
    11101 tttgagatct tagatgaaat caatcgttgt ccatcactta atgtagggtc catcgaatgc
    11161 ccgtttacag taactggtgt aaacacgtac actctagcta ataaaatagc cgctaataca
    11221 aggatcaaaa tcccccaatt tttcaaaaat tctttcacag cttacctcct acttgtattt
    11281 tcctcgttat ttattacgtt ttttcacaat acttcgttgt actttcttta ttctacaatg
    11341 tttataaatg aaaggaaaga ataagtgatg tgttcgtctt atctttctcg ttatataaaa
    11401 aaggaacgtg acaaaagacg tgtcacatcc cccttattcc gaataaacgt ttttatcgca
    11461 accactgata cacggcgttt catcacatta tgaatgtctt cttctttggc tacgactata
    11521 aaccactggt tcttcctgcg actgtgcctt ttttgcaatc aatttcagac gataaggctc
    11581 atgatagcgt aacccgtctt tgacaaaatc accgccgacg atgaacaatc cgacaggttc
    11641 caatgtgtct tctgttgagt caagtcgaat ggtccctact ttttccatgt ccgagtagct
    11701 tcgtccaata tttagaacat actctttctc tcctgttttc ttgacatagt aataaggata
    11761 caatgtattt ctatcgacca gaaatccatc catgcgaccg agttcatttg ttttgatctc
    11821 accatttcca atcaaatacc gcatattctc ggctaatttc aattgataat cttcgtcata
    11881 agaaaccatg acctcttgtg tattcacttt atcgatcgaa tttccattat ctatcgattc
    11941 ttctgtctgg atgttgaacg cttcatcagg taaatacttc acaaaaagct gagtgacatc
    12001 ggctttcgtg tttttatcta aaaagtaata aaatacattt aaaacagtaa aataaacaag
    12061 cactagaaat gcaagtccat ataagaaaaa ttgaagatag ttttggtttt gccaaagtcc
    12121 ataaacaatt ctcaataaat aaagcgtggg aatcacactt aaaatcgtat atccacggtt
    12181 aagatattta ggactgatgt ctaaatagcc aaggaattta tgcaccccgt tgataaggtc
    12241 tagtaaaaat gtcattgaga atcacctgct tgctggttat acgtttgggt atttcctgaa
    12301 tcttggtttt gctgtgctgt acgtgctgca tcatcatttt gcgtgttatc atctacagtc
    12361 gtactgcttg gtatcgtttc ggagctagca cttgtatccg gacttgtact tggattggtt
    12421 gaatccgatg gagtgccatc tggtaccgta ttacttgttt cgcttgagct gtcagttgta
    12481 tatgtctcat catacgacga actagttgtt gtatcgtccc atgtacttgt cgtagtggtt
    12541 tcattcaaac tttccgttgt tgttggttca gatgtcgaga ctgaagtagt tggtgttgtt
    12601 tcttctacag tactagaact tgaacttggt gcacggtagc tggaactatt cacaatacct
    12661 gtagtagtcg cgacaatcgt cgagattgtt aatacagcga ttggcttcaa aataggtgct
    12721 acttttcgca tcttatctct tccttcattg aaatttattc ttgtttcctt actttactac
    12781 ggacaaatta tacagtctac cttatttcta ccataaattc tttatttttt tcttacgttt
    12841 ttccgaaaga tttctttaat tttcctcaaa aactacggtt gatgttttta agtaagcttt
    12901 tgcttcccga ttgatttctt tcagatcgca tccttgctgc ttggcagcga acacgcaacc
    12961 acaataacat tggcgataaa tatcatactc tttgcacatt tgcaaagagc gttcatagcc
    13021 tttgtttttc ttgaaatcac ttggcaagta ttgtgtcccg taaatctttt ggatatccat
    13081 tccgatctga ttgatcaaag ctgcattttt ctttggcgag atggtcaggg cacttccaaa
    13141 gtaatcgtat cctagttctt gcgctttttg cgcaacaaga tctaatcgca tattgaagca
    13201 tgcactgcag cgagcaccac cttctttttc gttttcaaga tggtttgtta aaaccatttt
    13261 ggtgaattca tttggacggt attcatcaac gattagcgca acatgatggt cagttttctc
    13321 attaaattgg tctacaaatc gtttttgttc atgtaatctt cgcaaatact cacttttagg
    13381 atgaatattc gaatttgaaa aaaataacgt gatctctgca tattgagaca tgaattccaa
    13441 tgtgtgcgta ctgcaaggag cacagcatgt atgtattaat atcttaggct ttacttcttc
    13501 tttctcccat tgcatgatca tttttttcag aactgtatca taatttattt tttgattttt
    13561 cattttttct acgatttgtt gagcattaat caatttgtta ttcctcctcc ttcttttttc
    13621 taggattttc cttattataa gccatttcta ttttaatcga aagctgtatt gttatctgtt
    13681 tgataatttc tttgagtatt cctaattccc cttgtatact ggtagaaacg actattgtca
    13741 ggaggatagt actgtgcatg ttgatttatt ctttcgaagg ctgcagcggc gaggacagaa
    13801 gtttgctgcc aatcatttct catcgatcca aatcatcgtt ttttattata tattgatgac
    13861 ggtcttatca ctcgttctat tttatatgcc gatttttcgc gaaccagata gccatgtttc
    13921 ttttgtggac atgctgttca tggcaatcag tacagtgagt gtgactggac tatcgacttt
    13981 tgatatcaat tctgtgttca atgataacgg tattatactg ctggaaatat tatttcaagt
    14041 tggtggtttg gggatcatga tgatttctac tgcttttgtc attttttcta aacgacggat
    14101 cacactgaaa cagcgacagc tgatcatgac ggatatgaat caaccgcgtt tgtctggtat
    14161 cgtccgactg attcggatca cttttgctat tttaatctgg tttcaattac tttttgggac
    14221 attcttttct atttattttt attacagagg ctattttgat cgttggcgtg atgccgtttt
    14281 ttacggtttt tatcaggcga tatcggctgt cacgaactct gggtttgacg tcactgggga
    14341 ttccatcaag ccctttgcac atgactattt tttcttgatc ctgatcatgt ttttgatttt
    14401 tgtcggagga attggttttc ctgtattgat ggagtgccgg gaatggctat tatacaaacg
    14461 ttccaatgcg aagcttccgt ttcgtttttc cttgtttacc aaattagctg tgttagcttt
    14521 tgttatcttg tttgtttcgg gaactgtctt gatttatcta ttggaaaagg accatttatt
    14581 ccaagattcc aatatgagtg tcaaatggat caattcgatg ttttattcga ttacgactcg
    14641 taatgctggt ctgcagattc acgatttggg tgattttcaa atcacaacac tgatcatttt
    14701 ttctttactg atgtttatcg gatgcagtcc tagttctgta ggcggcggga ttcgaacaac
    14761 tactgtagca atcatcggtt tgtatctata ttccttcctc aaaagtgaag ataacatcaa
    14821 tatttttgga cggagaatcg atcaagatga tgtgcgaaaa tcagtcgttg tctttatgct
    14881 ttctcttggt atgtgtttct tttgtatcgt ttttttatct gctacagaag aacaaacgct
    14941 gatctcgatc atcatcgaag taacttctgc gtttggaaca actggtttat ctttagggat
    15001 taccggagac ttgtctgttg tgggcaaaat cacgattgct acactgatgt ttattgggcg
    15061 gatcggaatg ctatatacat tgatgttatt cgtgccaaaa gaaacacgag atctaggcta
    15121 tgaatatcca agcgagaaaa ttattatcgg ttaaaatggg aatataggta agagggaata
    15181 ataaaatcac agcagcttga aacaatcatt ttgtttcaag ctgttttttt gttaaatcat
    15241 gcgattttgt ccgatttcat gcactttcag ccgtaacctt ttcttaagaa taagaaaagc
    15301 aatttaacct ttttttcaac atatggtatg attagttgaa tttattcgtc agcaaatcgt
    15361 ttttggaggt tacataatgg gaatacgcag tcattttgcg attgcagttg gaaaaacgtc
    15421 gcaatgggca ttaaaaactt tttttaaagg cggatcaagt ttaccaggta aactagcgct
    15481 gaaagtcgat ccacatatac tagatagttt ggcaaaagac taccaagtcg tcgttgtcac
    15541 cggaacaaac ggtaaaacat tgactaccgc tttaacagtc aatattttgc gccagcaatt
    15601 cgatgaagtt ctaaccaacc ctactggtgc gaatatggta cagggaattg tatcgacttt
    15661 cttgcaggca aaaccaaaaa aaggacagaa aaagtttgct gtcctagaaa tagatgaagc
    15721 aagtttgagc aaagtcacag aatatatgaa accagaactg ttcttattta caaatatttt
    15781 ccgtgatcaa atggatcggt atggagaaat ctatacgacc tataaaatga ttgtagacgg
    15841 tgccgcaaag tcaccaaatg caacgattat cagtaacggt gattcaccaa tcttcaattc
    15901 tatcgaaaca gtcaatccta gaaaatatta tggctttgac catgaagaag atcgtgaaca
    15961 aatggctcat tacaatacag acggtgtctt atgtccaaat tgtcatcaca tcttgcatta
    16021 taaaatgatc acttatgcaa acttagggaa atattactgt ccaaattgtg acttcaaacg
    16081 tcctgaattg gattatcgat taactgatat ggtacggatg gacaatgtgt cagcagattt
    16141 cgtgatcgat ggtcatgaat acggaatcga agttggcggt atgtataatg tctacaatgc
    16201 tttagcagct actgcagtgg cagagtactt caatgtcgat cctgataaaa tcaaacaagg
    16261 tttaggatat gacgagaaag tattcggccg acaagaagtc atcaatatca atgggaaaaa
    16321 atgtacatta gtacttgtca aaaatcctgt aggtatcaat caagtaatcg atatgattgg
    16381 tctagctcct tatccatttt ctttagtggc tttattaaat gccaattacg cagacggaat
    16441 cgatgtcagc tggatttggg atggtaattt tgaaaacttt gccgatatgg atatcccagc
    16501 tgttatttct ggaggagacc gtcatacaga tatgacttta cgtctaaaag ttgccgggat
    16561 tccagaaaat aaattgacag aaacaaaaga attaccagaa gtaatcgaaa aaatcaaaga
    16621 attgccaact gagcatgtct atattttagc tacttacact gctgtattgc agcttcgtaa
    16681 agaactagct gctcaaggat ttatcaaagg aggaatgaac cgtggctaat tatgaactca
    16741 atatcgccca cctttatgga aatttgatga acacatatgg tgataacgga aaccttttga
    16801 tgttgaaata tatagcagaa aaaatggggg tttcttgcca tacggagatc gttagtatac
    16861 atgaaccttt tgatgctgat aaatatgact tggttttctt tggcggtggt caagactttg
    16921 agcaattaat catttcaaaa gacatccaga aaaagaaaga ttctttgatc aactatatcg
    16981 aaaatgatgg cgtcatgtta gccatttgtg gtggctacca attgctaggt cactactaca
    17041 tgggagctaa tggagaaaag atccaaggaa tcagtgcact ggatcactac acgttaagtc
    17101 aggaaaataa ccggtttatc ggtgatatcg ttatccataa cgaagaattc aatgaaactt
    17161 attatggatt tgaaaaccac aatggtcgga ctttcttagg tgaaggtgaa cgacctttgg
    17221 ggaaagtggt acaaggtcaa ggaaataatg gtgaagacca atctgaaggt gtcatttacc
    17281 gaaatgtctt cggttcttat ttccatggac ctatccttgc aagaaatgag aacctggcag
    17341 ctcgtttgat ccgcctagct ttagaacaac gttacggtaa agaattggat ctgccctatc
    17401 ttgaaggaca aacagcctag ttattcgaaa aaggggaatg tgacaaaaat ttcgtcacat
    17461 tccccttttt cgtatcctat tttaagcgac ttgcttggca taacggttca cgttttgctt
    17521 gatattcccg attgttttcg gatcattgat tcctgtgact tcttgaatca attgttccaa
    17581 gttttcatgg atcttcattt catgcaattg acggctttgt tcatcttcag gatcatagta
    17641 attaaaaata atccccattg ttgccgtcaa atgtgggcaa gtcaagttat gttcttgtag
    17701 ttctctcact ggacgtgtaa atctttcttg ataacctaac ttacgtaatg gtgtccgagc
    17761 gacacgtgtg atcgcatcag agatattttt gttttggaag cgatgaatga ttttttcaat
    17821 atacgcatga tgctgctctg catcaaaccc ccatttggca atcagcaaac ttcctgtttc
    17881 ctgcaaaact gattttaatt gtgccacaac taacgcatct tgcattgctt catcaatggt
    17941 ctggtaacct aacaaggcac ctgtataagc gacagtcgca tgaccagtat tgacactaaa
    18001 tagtttgcgt tcgatatatg gttctagatc atctacatat aaaacccctt ttaattggat
    18061 ttttttattt tttctttggc tatcgtcaat gacccattct ttgaaaggtt ccacttggac
    18121 gaataaagga tcttcatggt gttgtaatgg gacgattcga tcgactgctg catttggaaa
    18181 gccgatatat ttgtcagtaa atgatgtttc cggaagatat ttatacactt ctttttctag
    18241 aaatgctgaa ccgccaatca tattttcaca ggcaatgata tctaaagatg ttgtgcttcc
    18301 ttccttttct ctttcttgga taccttttgc aatcaactca gcaataaaag gtaagatatt
    18361 cgggccaatg gcggtagtaa ctaaatctgc ttcagcgatt tcttggatta ctttttctgg
    18421 atttgtttga ttgttgattc ctttgacatt ttttacagtg attttttctt ttgattcatc
    18481 tgctaattcg atttcgtatt cttttctttc atttaaagca tcaatgatcg tgtgattcac
    18541 atctacgaaa gtaatctcaa atccattagc tgccaaaacc tctccaataa atcctcgacc
    18601 gatatttcct gcaccaaaat gtactgctct catttttctc tctccttaag caattgctag
    18661 attttccgtt acttcttctt tgctgagcgc gtctgctagc tggacaacat tgtccatatc
    18721 actgcaataa agtgcgattt gctgtacaag ttgcaaatgc tcatctccga ctcctgcaat
    18781 accgaacaaa acagtcgcaa tcttttcttc ttcttcggta ccaaaatcta caccatctgg
    18841 tacttgaacg acacagatgc cggatttttt cacatatttt tttgcttcat ctgttccatg
    18901 aggaatcgcg ataaaattcc ccatataaac agataacatt ttgtctctat ctaccatggc
    18961 atcgatatat tcctcatcaa cgcaccctgc ttctacaagc atctgaccgc aatagcggat
    19021 cgcttcttct ttggttttaa aagatcgatt gagctcgatc atgtcgaatg gcaaactttg
    19081 catgctgttt tcctccctta agctgtctgt ttcaaatatt gtgcaatcga ttcgtatttt
    19141 tgcggattga ccatgctgtt gactgataaa tgaatggatt gaggtgcagc catctgtact
    19201 ttttccgcta aactttcttt ggctacgatc agtgaatcag gttcatcttt taaatcttgg
    19261 atattgactt ttcttaacgg gatagttaaa tggttcttgt ctaatatatc tttgagcacg
    19321 gtcatcccca ttgttgctga tccggaacga tcatcgtgga caagtagaat ctctttgatt
    19381 tgtttgatgt ttgctgaaag aaactcttct gctggctcat tctcaatttc agaaactgat
    19441 gttttctgct tgctgcttaa ttcttgaatg atttcatcat atcgaggtga attcaaaaag
    19501 ttttctactg agacaaaacg cgcgctcggt gctttttgtt ttgctctttc ttgtagttcg
    19561 acctgagtaa cgatcaatgt attcgagtca tctgtcagtt ggctgattgc ttgattggtc
    19621 accgtttgag gcaagtctgc ttcctgtact ttttttctca agatcgaagc gcccatcgca
    19681 cttgatccca ttcctgcatc acaagcaaaa atgatttttt gtacgccaga gaatgtagct
    19741 ccttgaggaa taccttgggc acctttggaa gcttgcttag ctgcctttgt ctcttgaaca
    19801 gatttttcga agtcttcatt tgtatctttc gcatccgctt tcaaaattac tgcagaaata
    19861 ccaaacgaaa cagctgcggc aacaatcacg cccaaaataa caggaaggta agaattgaac
    19921 ggtgtcatcg ctagaatcgc aatgatcgac cctggagaag ctggcgctct tagtccagca
    19981 tccatcaatt ggaaaatgaa acttcctgac attcctccgc ctataactga taagaataat
    20041 aacggtttca tcataacgta aggaaaatag atttcatgga tcccgcctaa aaagtgaatg
    20101 atcatggctc ctggtgctga agattttgct gctcctttgc caaacaaagt aaaagcaagt
    20161 aatacgccaa gtcctggacc aggatttgct tcaagcaaga atagaataga ttttccagcc
    20221 tctgctactt gttcagttcc caatggtgtc aaaatcccat gattgattgc attattcaaa
    20281 aataagattt ttgctggttc aataaaaata cttgtcaatg gaatcaaatg ggcagataaa
    20341 atgatctcta caccttttgc cattgcttga gtcaatgcat cgacaactgg accaatcgca
    20401 gaaaaaccta ataacgctaa tccaaaaccg ataagacctg ctgaaaaatt attgactagc
    20461 atttcaaaac cggatttgat ccgtttttgg aatagctgat caaatttctt gatggcatat
    20521 ccaccaagag gtcccatgat catcgcgcct agcatcattg gaatatctgt tccgacaatc
    20581 acacccatcg ttgcaattgc cccaacaact gatccgcggt caccagcaat tacttttccg
    20641 ccagtataac cgatcaataa aggaatcaga taagttagca ttgggcttac catggtagcg
    20701 aaagcctcat ttggtaaata accatctggg ataaatagag ccgttaatac accccaggca
    20761 atcagtgctc caatatttgg catgaccatg cttgataaag tactgcctaa tttttgtacc
    20821 ctcgctttta gcgaaatgtt gtttttggga atcgtccgtt ccattttgtc tctccctctc
    20881 tccttttgat actctaattg tatccgctta ctaaaaagag aaatagtcgt actttggcac
    20941 gaaactttgt gccaaaggtt tgagtatgtc agcaaaataa aaagatttcg gtatcagaaa
    21001 acttcaattt taaataaaac tgttatttta atttttttat aatatgcaaa caataacttt
    21061 tgttagcatt taatttagat gttttccatt ctattatgtt attaaatttt atattggagg
    21121 cctatctatg acattactga tcaaaaacat gaacatgaaa cgtggtaaaa aacgtgtgat
    21181 tcgacaattg aatcttttag tagaatcagg agaagtagct gctttgattg ccccgaacgg
    21241 atttggcaaa acaactttgc tgaatggcat tgcccaactg ctgccaacaa cttatgatca
    21301 attgtccctt cacaacatct ctcctactgc ttatacagat tatcgccggt ctttcttttt
    21361 tctagaatca acgaatcagt tatccgacta tctatcggct gttgattatt tgactttgat
    21421 caaatactat tggcagagca aagtagaatg ccaaactgtt ttccaacgat tgaagatgac
    21481 tgagtacctt actttgccga ttggaaaaat gtctctagga atgaaacaac atgtcttgct
    21541 tgctgcttac ttgatcagtg attcatctgt catgttattc gatgaaccgt tgaacggatt
    21601 agacccagga agtattgata tcttgaatag cctcatcaga caatggcaga aagaggggaa
    21661 gacgattctt ctttcttccc acgctatcgc gaatatccaa tcgatttgtt ctaaagcatg
    21721 ttttctaaaa aatggagaaa tcgtcacaga atcctcaaat atggaagaga ttttagctat
    21781 ttatcgtaaa ttatatgagg tgtaactatg tctttactta catttgaacg caatctctta
    21841 ctaaaaaaca aagtaaactt tttatttcct ctattgttgg tcgtgctctt tgcattccca
    21901 cttttttttg atcataaact tgcttataca gaattcgatg aactgaatca taactatgaa
    21961 gagatgcaac gtttgattga aacgttgaaa gaagatgaga atgagaaaga atttgtggaa
    22021 agtttagaga aaagcaacaa actgatcgaa gcaatcttac atgcaaaaaa cacagggaat
    22081 gtccagcaaa tggtcgaagc aacttatcat tatgaaaaag atatactgga tcgactgatt
    22141 tctggtcaaa gacaaggaat tccaatcatt gaacaacaaa aacgtgtcga gttgctccgt
    22201 tatatgaaag aacatcagat ccaaagatat tctatttttg acttgccagc gcatctctct
    22261 ttagcaaatt actacgaaaa tattttttca ggaatgattt ctagtttttt gattttatgt
    22321 ataaccgcct tgtttctttc ttccatcatc tcttatgaaa aaagaaagca ggtaatctct
    22381 ctagtcaatc tattactgga tagtatggta aagaaacata gcatccgttt taccatttat
    22441 tatggtgcag ctatgctaag tctcgtgatg ccatttttga tcgtctctat actagtaata
    22501 atcaaaaatg gtttgggaga cttccgttat cctgtaggaa cgatcattgg tcaggagata
    22561 cgaatcctgc cgatgtatga gtatcttttc cagagctttc tgttcctgtt gttgtgggtg
    22621 ctctttttaa gtacaatcag ttttcttcta tctgctttat ttgaacattc tctagtcaat
    22681 cttttaggta cacttctttg tcttttctta gcagagtatc gcttgttttc ttcgattgga
    22741 tggatagaat caatcagtca ttacttgcca acttcttatg ttgattttca aaatgtgatc
    22801 ataggaggag atattttctc gccactagct tcagaacagg ttactttcat gaatggtatc
    22861 ttgacacttg gtatatggag tatcgtttta ttattcatag gtatgggcac tatctatata
    22921 aaaaagagct attaaaacac aaaaataata ggggcggtga caaaaacctt gtcaccgtcc
    22981 cctatttcaa ataaacggtt ttctagaata aatagttttc aaaagcttgc atccgcagta
    23041 ataagaacct cccaacaaaa atagctcctc ttatttttat cttggttgaa gttattactt
    23101 gatgcaaggc agcttttttt actgtgaaca ttggttatat atgtccatgt tcaaaaatca
    23161 tttggacttc ttcaatggtt tccgcatcac tgagcgccat tacttggtcg atgtctgaac
    23221 agaaaaaggc taaatcttgg agcaccgcta actgatgttc tttttttaaa gccactacaa
    23281 ataagagtgt cgcgattttt ggttcctctt ttgttccaaa gtccacacca tcaggcactt
    23341 ggactaaata gattccttct tctactactt cttcatcttt gtcagtccca tgtggcaagg
    23401 ctacgaaatt cccgatatag acactcgctt tttcttgtct ttcgatcatt gattggatat
    23461 atgccggttt gattccccct tgttgggtca tccactggcc gatctcggtc aacgcttctt
    23521 tccaattttg ataggattga tcgagcaata ttttttctgc tggtagctgt atcatctttt
    23581 caccttcatt tctttatttc ttcaatcagg attttcgcta acaactgata aacgatttct
    23641 ttatttcctg agcggaagat ctcggtgttc aagtcattca tgatgattgc tccactgatc
    23701 tttcctaaaa gaatcccgtc tttttcttgg attggcgaag gcgccagcat aactaacata
    23761 cgggacaaat cgactggctg tctatccatc ccttcaatgg tcaacggatg tttcaaattg
    23821 aaaatacaaa agatcggttt agaaatcaat gagctggatg tatggaataa ccccatattt
    23881 gtatgaggaa taccgatagg tgcttgttca tatcgcttaa gcagccggcg atgaaccacc
    23941 tcttcatccg ttactagtcc ttcaggaaaa taaggaaata agctgcgaat cgtttcggaa
    24001 atcgtctcag gattatccag ttcttggata aaaaaacgac cgagtaattg attgatttga
    24061 tccatgaaat gcattgtttc ctcataagta tcttctatca tccactcgtt attgctgtta
    24121 tacgtaattt tttgcctttt tttcttctca atatgcgaaa aagcttcttt tagctgcttg
    24181 acttcatctt ctagcagtag cggggagact aaaatatatt ttccaccgta tcctggtaaa
    24241 atagatgtag aaataacaac atcatattct tcttgtaaat cgattttttt taaatcagca
    24301 atccgataga aatgaatttg ttggatgaag ggaaaatacc ttttcaatcg tagttccaac
    24361 atgctagtag aagctaatcc acttggcgaa aatcctgcaa tactgatatt gctttctttc
    24421 ggacttcgtt ccaatgaatt cgcaaaatga aggaccatat aagcgatctc ttcttcggat
    24481 aatttttttt cttgaaaaat ccgctctact gctttacgga ttgcattcgc aacttcctcg
    24541 tattgaacca tgattttttc taaaatcgga ttggtcagtg tctcttcttg taaaacggct
    24601 cttgaaaaca caccagataa atgagtcaac agcattttgt acaaagtttc atcttcataa
    24661 aaatggacat tcgtctcttc acttacttct tgaatcagtt gttttactaa ataagcaagc
    24721 tgtgtatcga acgtctcttc aaagaaattc tgatcaaatg agttcgtgaa gtcatttaat
    24781 aatcctgcaa aaaagacgat ctcatttact ggataaagtt gttttgtctc agaagcgatc
    24841 cgtgaaaaca atcgtttagc tgtctgtagt aattctttgt ttgccagact attatatgtc
    24901 tcatttgtca gtaaatgatt tttcgtcaca cgctccatcg tcaatgtcag catcaaaacc
    24961 aaatgttcaa gctttctatc acttagattg gtcttttttt ggatcaattc ttccccgatt
    25021 actttgcgtg caagagatag atgttgcgga tcaacgaatc gaaaaaaata attcagtgat
    25081 gcatcgaaag aaatactatg aaaaagttca tattcattga tttctaattc taatacattc
    25141 gctgtcagaa gccggcggta tttttccggt ccttcgatct cgtatccctg gttccgaatg
    25201 atttccaaag gcagttggcg aatactttct tccaactgtt tgatatctgc ataaaaagtg
    25261 gtattactga tgcgatatga ttccatgaaa tgagcaagac tcaacggctc tgtcgtcagt
    25321 aacaactcgg ctaaaatcgc gtgttgtcgt ttagcagtgg ataagtcatt acttttccca
    25381 tctgtatttt cttttaattg ctctaatact tcttctgatg cctcaatgac aaatgagcct
    25441 ctagctgctt ttttcaaaga agcatttacc gatttcaatg agacttcaag attatctaat
    25501 tcgcggtaaa tagtccgttt actgaccttc aataaattca tcattttttg gatagataca
    25561 ggtgacgggc tactgagaaa ttctgctaat aattgttttt ctctgggcga taaatacatc
    25621 ttatatttct cccttcacga gtttttcgta ttcattcgta gctacccaat gttcaactag
    25681 aactagccga gggatctttt cttctagctg ttcagttaca gctagttctc tttgtgcgat
    25741 ataaatcgtt tcttcttgga ttttgatttt ttctattgat tcttttgaga taacaaactg
    25801 ttttttcgct ttttttgcta attgacttaa aatcgtcatg cccatagttt gtgagccgcg
    25861 tctgttgttt ttatacaaaa atacaatatt agcaggcgac gttttttgtt ctgtgatatc
    25921 tgctatctct tttttttctt cccgctttgg aaattggatc gcttctttta ttcgtgacag
    25981 caagatatct gtttccaaaa agtgatccag tacatagatt tgactttgag gtactttttc
    26041 tcttgcaaga cccgctagtt catgttgcac gataaccagc tgatttactt ggttattcaa
    26101 ttcatagata gagctgtatc cgacagaaag atcgattttt tctgctgcta atttcttttt
    26161 cagcagggcg gctcccatag cacttgatcc catacctgag tcgcaagcaa aaatgacgga
    26221 atgaatgata gatggttcca agatttctcc cctttctttg accgttttgt tctctttttc
    26281 tttatttggt aatttcaata gtatcgctgc acaaatgaag gtcacaaatg tactgattgc
    26341 tatccctata cttaccccag ctagttgtgg ataaggcgca ttggcaaaaa tcacgagtag
    26401 tgacccaggc gatacaggtg cagctaatcc cgtatctaac cattgaaata tcaacgtgcc
    26461 gcttgcccca ccagccatta cagctaaaaa tagatacggt cgcagcaaga caaatggaaa
    26521 atagatttcg tgaatcccgc caatcgcatg gatcatcaat gctccactcg catttacttt
    26581 ttcctgtttt tctgcaaaac aaaggataga aagtaagatc cctaaaccag gtccaggatt
    26641 tgcctccatc aaaaataagg aagagtaccc gactgtttgt gagaattcga ttcctaaagg
    26701 tgtcaataca ccatgattga ttgcattatt gaaaaatagt acttttagag gttctaagat
    26761 cagactgaca agtggtaaca gattcattgt gactagccaa gacacaacgg aggcgagcaa
    26821 caagtgaaca ctatttaaaa ctggttcaat aaaaacgatt ccaattatac aaataacgct
    26881 accagtcagt cctactagta ggttgctgac aagcatttca tatccagcct tgaatttcac
    26941 atggaatcta gaaacaaatt gttcatatac gatgccggca agcggcccga taatcatcgc
    27001 tcctacgatt tgtggagcat tggaacgagc gatcaaccct aaagaagcaa ttgcaccggc
    27061 gactgcccct ttttgtcccc ctaccgattt cccgcctgaa taactgatca agatgggtaa
    27121 aagcagctgg atcatccaat ggcatacctc ttctaatgtg gtgtgcaaat cgccagacac
    27181 accttttgtt gctaatgaca agattcccca agcgataaac agacttagat ttggcatgat
    27241 catttcattt aagtgatgtc cagcttttct cagttgtttc atagacacgc gactgctgtt
    27301 gtttgctttc catttgtatt ccatcttttt caccagactt ctatttattg atggacggat
    27361 tttatgtaga aaaagcagca aacgaaaaac cgtctgctgc tttcagctat ctgggaacta
    27421 tttgcctggt tcagacgcaa tgattgctag ttcaccttgg accgcccact cagaaacgcc
    27481 atttggaata atacagcttg ttcctttttc taatggatat gtttttccgt caacgacaag
    27541 gtctcctgct ccttcgatta ccgtcatcaa tgtataaggg gcttgttttt tgaaagatgt
    27601 gattccttta atatcccact catatacatt gaaaaactca gtttctaaat aagtgacgat
    27661 cgatgaattc ccttttcgga cttctttgat ttgtaattct ggtgttttcg cagggacagt
    27721 tgttacatcg atcgattgtt ggatatgcag ctctcttgtt tgtccttgcg catcttttct
    27781 gtcgtagtca tacacgcggt aagtcgtgtc agaactttgc tgtgtttcta aaatcatgat
    27841 tcctttacca atagcatgga tcgttccgct tggtacataa aagaaatcac cttttttaac
    27901 tggtactttc ttcaaaaggt catcccagcg cccatctttg atcatctcag ctagttcttc
    27961 tcttgtcttt gcatgatgac catagatgat ttctgcacca ggttcagcat cgatgatata
    28021 ccagcattca gtttttccta attcaccttc atgtttcatc ccataggcat catctggatg
    28081 tacttgtaca gatagatcat cttctgcgtc taagatcttg atcaataatg gaaacactgg
    28141 ttctgaagga tggccaaaca attccttgtg ttctgaccat agttcatcta atcttttccc
    28201 tttgaattcg ccattttcta ctacactgac accatgagga tgcgcactga tcgcccaatc
    28261 ttctccgatt ttatcgttag ggatatcaaa accaaagact gaacgtaaac gacttcctcc
    28321 ccaaattttt tcttgaaaaa ctggttttaa aaacataggt tcctgcataa ttcaaactcc
    28381 tctcattttt catagctatt atagcattat ttcggtaata ccgctttctc tttcaaaaag
    28441 ttttcaacaa tttcatctcg acatatgaga tactccgcac gatgatcgac aggatgtacg
    28501 cgatttgaca aaaatatcaa cgcttcttgt gtttttatat caatcaagaa aaacgtacct
    28561 gtataaccag tatgaaaaag aatcgcatga ccgtcttcta catccttttt taagtcccat
    28621 cctaaagaac gatagccatt ttttttcggt gtttgatctt gcagtaaaga atcaatcgtc
    28681 tcttcttcta aaaaacgacc attacggatt tttcccttgt tcaaatacat ttggacaaaa
    28741 gtaatgctgt ctttcaaatc aataaagaga cccgcgttac ctgcatgttc agccaaaact
    28801 cttgctttcg gatcatgagt ttgtccacgc aaaacctcac ccgaaggcag ttgttctgtg
    28861 ggaacgattt ttttggaagg aaaaggaaga aataagctgt tcatcattcc taatggttcc
    28921 aatacttgtt cttgaaagat ttccactgca gtttcttggt aaatttcctc tagcatcaac
    28981 ccaagaagaa tcgtaccggc atctgtatat tttacttctt ttcctagttg ctcacctggt
    29041 tctagcttca aataagcggc gatcaattct tcctgagata gcttatctct gtttggtatc
    29101 catgttttga tatcagaagt atgtgtcagt aaatgacgca aagtgattgt ctgattttca
    29161 aattcaggaa gatatttatg aagcggttga tcgagttcta tctttccatc ttctaataga
    29221 cgtaaaacta atgaagtggt tgcaacgact ttcgtcagtg aggctgcatc aaacaacaga
    29281 tcttctgtca tcagctgctt ttcaggaacg acttgggcaa agccggctgt aaatgtttct
    29341 acttgatttt cttttatgaa ggcgactgct acacctggat aaaatccttc ttccatcttt
    29401 ttcaaaataa gctgtttcgt gtgtggatac atctttttct cctgcttttc tatcagattc
    29461 ttacagtcca agcttaaagc ttaatctatg aggaatcaac cttttttaca caaaaaaact
    29521 gcgcgaaaaa ccgcgcagtt tcccccggta tactattttc tctttctatc tctagaataa
    29581 gcgatagtat gaccgttcat aaaatgacga gacatgcgac aatcgttaag aaagaaaaag
    29641 ttaagcgtcg catcgaatgt cagttttatt cttttctttt ggctcactga cgaaccacca
    29701 ttcgattcct atagcatcat agattgtgag tactgtgtgc tttttgtcta agaagaaatc
    29761 aactgatagt tgctgcaagt gagcaatcag tccttccatg ctttcagtat cgatcatgaa
    29821 tttcagaaaa tcaagtccaa tcacttcatc tgtaggcaaa tcaattgcgc ctccgctagc
    29881 ttctgtcaaa ccaacatgga aatctccttc attcaataca tggatgccct cttcttcatt
    29941 atgtaccttc aaaccaagta catgattaag aaattggcgt tcttttgtta agttagtaac
    30001 atttaaatgg attttttcaa aataagaacc tgttgatagt ttactgaatt tttcttcaga
    30061 ttttcttaat aatgcttctt gatccattgt ttctggaatt tctctttggt cgttcttatt
    30121 ctcttcatgg taaatttcca attcattctc ttctgggtct acgagcaaaa tccccatacg
    30181 tccatgatct tctaaagctt ctttgattgg ataattcatt ttacggatac gacaaaggat
    30241 atctgccatt tcttctgtgc tagggataat caatgaaaaa cggttcagct ttttgatcac
    30301 ccccatgtga tcattcgctc taggactttc ttccagcagc aacatttcac ttccagcttc
    30361 ttctgttcct aaaatggcta attcgttttc ttctcttttc aaatcaaagc caacaacgtt
    30421 cttgtaaaaa tcaatcattt tatctcggtc tttcacccga atggctacac tttttaaata
    30481 ggtggcagcc cccaattgaa aatctttcat atttcttgct cccctctacg tcaagcttta
    30541 ttttaacgat ggattcttct tctttcaagc aataactttg attttgttaa aaaaaatgtg
    30601 agaaaattcg cattttgctt gttttttgta aattaatgag taaaaactct tataaaaaga
    30661 ggttgtgaaa aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagctcctc
    30721 ctatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg
    30781 aagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt gaaaatggct
    30841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta
    30901 ccaagaggtt gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt caaaaaacag
    30961 cttctcaggt ttgttgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
    31021 taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca aaacggaaaa
    31081 atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
    31141 gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa aaaaataata
    31201 aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa agatctgcat
    31261 agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat
    31321 tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac
    31381 ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct gttcgtgtat
    31441 ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag aatgcccgcg
    31501 caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg tttaatacag
    31561 tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct ttagcagcct
    31621 caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca tagatacttc
    31681 ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg
    31741 aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca tcgtagaacc
    31801 gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca atagcgacca
    31861 tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct ggtgtgtagc
    31921 cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct ggaaggccag
    31981 atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg gctgaagcaa
    32041 ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga gagaactgtg
    32101 ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt gcaactttta
    32161 ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc atcaacggca
    32221 tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc tgattttctt
    32281 tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg gtatgaccac
    32341 catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga tctgcgaaaa
    32401 acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg atcatcaata
    32461 acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc ccaatcaatg
    32521 tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca
    32581 tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa taggaaatcg
    32641 gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt cgttgggaaa
    32701 cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga
    32761 ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt gttacttgtt
    32821 ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct gtggttgctt
    32881 tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct agtacaaata
    32941 cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt accgttcccc
    33001 agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca ccatgtcctg
    33061 gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta agtcctcctc
    33121 tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata gttcttcatg
    33181 tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg catccattac
    33241 tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg ttttcaatgc
    33301 ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat caaacacata
    33361 gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt gtccaccaga
    33421 gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct ctgaaacaaa
    33481 atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg catccggctt
    33541 accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt gaggaacaaa
    33601 gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc catttatttg
    33661 gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg tcgttttacc
    33721 tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc
    33781 ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga aggccaatgt
    33841 tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga taccgatttt
    33901 ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca ccatgataaa
    33961 aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca taaatgcaat
    34021 cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc
    34081 tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga cgatcgtatt
    34141 aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat tttcttcaaa
    34201 gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc gaaataccag
    34261 gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa agtacatgat
    34321 tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg tcaactgatg
    34381 atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat
    34441 acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg ttcttgtgat
    34501 caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg attttttata
    34561 gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa aaaatgtatt
    34621 tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca
    34681 aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg taagtgtcgg
    34741 caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa ttgctgaagt
    34801 caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt tatatactcc
    34861 gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc
    34921 ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag tattcatcac
    34981 taattggatc agtaattcca attcatgatc attttttact ttcagcttcg tccgatcgat
    35041 caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt gaggcgtttt
    35101 ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt tcataaacaa
    35161 gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt tagaaaaata
    35221 ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta acatatcttt
    35281 agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt tatcttcaaa
    35341 atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag caatcgaagc
    35401 ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc ttttttgttt
    35461 ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg attctccttt ctttcaagat
    35521 gacacattgt catatgacgc tatgtcatta taacacctcc tacctgtttt gcaagacatt
    35581 tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg aatccttcca
    35641 tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg atgagataaa
    35701 tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct gacagcatcg
    35761 ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac tcgctttcta
    35821 tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa ctttattata
    35881 ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact gctaatggtt
    35941 ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat
    36001 tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc
    36061 tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag ccttttcttt
    36121 ttcgagtaaa ctgattttgt tagtcatagt tataacaatc atagcaataa aaggagaagg
    36181 taagcatatg gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg
    36241 cacagttaag gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga
    36301 agtgactgtt ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc
    36361 aggttgggaa gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca
    36421 aaaaattgct cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat
    36481 cgcaaacgcc ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt
    36541 caatgtagct ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg
    36601 taaaaaagca ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc
    36661 tcaatatgta aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat
    36721 cgaaggaatc gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa
    36781 agctgctgct ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga
    36841 ggaaaaaatc ttcaaagcag catttgtgcc ttggatattt tcccatctgt ctctttttgt
    36901 tattctttct gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat
    36961 tcaaaaacat gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca
    37021 ccgatgattc cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg
    37081 attgctaact gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct
    37141 actgcaaaaa cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag
    37201 atagccggaa tcatcgctaa aaatgatcct aagtaaggga tcaggtttaa aaaacccgca
    37261 ataatcccca acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg
    37321 attgccacag caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc
    37381 atttcactca gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa
    37441 taaggtgcta attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc
    37501 agagccacta aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc
    37561 ttagatatat cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc
    37621 aactgttcgc gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca
    37681 atcgtatcta catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta
    37741 gggatgatca ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg
    37801 atactgtaaa gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag
    37861 taatacaaga ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt
    37921 ggtgcaaaca aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca
    37981 acaagtaagg ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt
    38041 ctgttcttct tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc
    38101 ccaaaccaat agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt
    38161 cttctggaca agcatcaaaa atcaatgata cgtgagctac tgtctttaaa ttcccattgg
    38221 ccatcggacc gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg
    38281 ctttcaaaga aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg
    38341 cagctgttgc tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg
    38401 tcccaatttc tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa
    38461 acagtataac acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac
    38521 gaaaactatt tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag
    38581 gaacctatac gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag
    38641 aagaattagc caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa
    38701 gtaaagctgg caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt
    38761 acgataagga ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg
    38821 tagcggttga tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa
    38881 atgtctacca tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag
    38941 aagcgacagg tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc
    39001 ctgaccaacg attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt
    39061 ccgaaaacgg caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc
    39121 gccatcttgt ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca
    39181 cagtccgcgt gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat
    39241 cgaccttgcc agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg
    39301 atggcaagtt cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt
    39361 ttgaagatgg ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc
    39421 gcgatttcgc actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata
    39481 atctgacatt atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg
    39541 tctatgtacc agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa
    39601 tgaatcagca agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg
    39661 ggaaaatcga agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac
    39721 agaactactt tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct
    39781 gaaaagaaca tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa
    39841 aaccgccgag atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac
    39901 aaggttgttg ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata
    39961 aaggtaatat tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt
    40021 tattttttca acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct
    40081 tggtcttgtt ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg
    40141 atactttgga tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt
    40201 ttatgaatgc ccattgcttt tgctggtgga cgcaaagtac tgactttcat aagcattgcc
    40261 cgatattttt ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc
    40321 agttcacttg tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt
    40381 ccttctccga ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca
    40441 atattcggta acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt
    40501 gtttttatcc tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca
    40561 ttcttattcg tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc
    40621 atattggatc aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc
    40681 tttataccac tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc
    40741 taatgataaa agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt
    40801 cgctgcaaaa taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc
    40861 tttactgtcg aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg
    40921 gatcgtatct gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct
    40981 agaaagattc ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc
    41041 ttgcagataa ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc
    41101 tagattatca tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa
    41161 ctgaggtgtt ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga
    41221 aatatatgga ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg
    41281 gtcaaatcct aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg
    41341 gatcgctgtc gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc
    41401 catttttggt acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga
    41461 aaaaccagtc aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag
    41521 catattgcca ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga
    41581 tacttctacg ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat
    41641 gttcggcgac tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc
    41701 agtgatttct ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt
    41761 cagattgtac aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta
    41821 aggtatccat agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa
    41881 attactgata taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg
    41941 gaatcgcctt ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt
    42001 tcctgcggca gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc
    42061 gatttcagtg atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt
    42121 catataattt gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg
    42181 gacagatcct gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa
    42241 gaagaatttt tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc
    42301 attttgacac cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat
    42361 tgctgctgtt ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt
    42421 caaatgcttg ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt
    42481 acgaaattcg aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct
    42541 ttatggcctt tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag
    42601 ctgttgtttc acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta
    42661 gacatgctca ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag
    42721 cagagaaaat atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa
    42781 cggcgcttgg aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa
    42841 caaaagataa tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg
    42901 agtcgatccc cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc
    42961 tggaaaatca gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata
    43021 tttatcaaca atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc
    43081 tatattcgcc tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat
    43141 gtaatccgag gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc
    43201 atcgcggata gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt
    43261 tgtgcctatc gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac
    43321 ataagcactt cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac
    43381 catacgactt gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc
    43441 ttttactgca ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc
    43501 tttttcaacg ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg
    43561 tgtaagccat tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc
    43621 atctattatt ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc
    43681 aatcaaatga acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg
    43741 caattagaca actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag
    43801 taggctgagt gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga
    43861 gcaaaatatt gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat
    43921 aagactaaat ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa
    43981 actaagtggc tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa
    44041 ttttgcccac gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc
    44101 gccacctgcc tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa
    44161 cacacgtgca acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt
    44221 gatcagcagt ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc
    44281 tttcccatca aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac
    44341 aacgattctt ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa
    44401 gattgcttct tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt
    44461 ccctcgagcc atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc
    44521 agcaaaaccg tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat
    44581 tccatacagg tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg
    44641 gcgaataccg atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc
    44701 ataatcacca tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc
    44761 ttcgtctgat acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt
    44821 ccaaccaaat tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat
    44881 gttgtcacca aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc
    44941 catccaatca gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt
    45001 attttgttta tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg
    45061 atttaaattc atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt
    45121 agctaaatga agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg
    45181 tgagaaagta tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt
    45241 catgattttt gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg
    45301 caattggcca ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg
    45361 cagtgcttct tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc
    45421 aatattcaac ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc
    45481 gtgttttcgt aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt
    45541 gagctgtctg gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac
    45601 cgcactttgc gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg
    45661 aattgctaag ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc
    45721 atattcgtac ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc
    45781 ataaaaagta tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca
    45841 gatcgtcgcc catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata
    45901 taaaacagca tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt
    45961 gtccaagtcc acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg
    46021 tttgataacg aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt
    46081 gacgagtccg tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc
    46141 cagcattttc tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga
    46201 tcccctcacg gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg
    46261 tgatggtgat tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact
    46321 tcccatgtcg atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc
    46381 cggctgtgtc catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa
    46441 cttctggtgt tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag
    46501 atttcaatat atccattccg attctcattg caccaaaagc actggctaaa tggatccgca
    46561 tgaaattcgc taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat
    46621 ttactcctgt aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg
    46681 tatcgccttc tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca
    46741 tacccaaaga attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc
    46801 agttattcgt atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt
    46861 ccggatggac attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag
    46921 ccgaaatgtt ccctgtccct ttggctacac tgttcgtagc gaccataccg gttcctgcat
    46981 ccccttctgg cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat
    47041 aagcccctga ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa
    47101 tgtgatccaa gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca
    47161 tttcttgatg gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta
    47221 cgcctagtac tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa
    47281 aattaatttc tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac
    47341 tccatctctg cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag
    47401 tgattgtatt tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta
    47461 gataaccgtg catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat
    47521 agatttcgaa tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat
    47581 cttctaatga ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag
    47641 cggcaatagg agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact
    47701 caatccctaa agatgtacga ccatctatga catctaacat actttgcgca tttacttcct
    47761 tcataatgat ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg
    47821 cttacaatga ttattgtata cttttgtacg tacatgtcaa gcagttattt acaagaatac
    47881 aataaaaaac acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct
    47941 gatcaaccgc cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag
    48001 tttcctcatc gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata
    48061 ttggtgttat gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag
    48121 cttctttgat aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt
    48181 tatctggtct tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta
    48241 tttttatggc tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga
    48301 ttgcagcaag gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt
    48361 atatgtctga aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga
    48421 tgattgcttc tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gggttgcctg
    48481 aaacgatggc ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt
    48541 tcggtgtttt agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag
    48601 aggcaaaaag agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac
    48661 aaatccagct gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt
    48721 tagaaaaata tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc
    48781 aaggagctaa tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg
    48841 cagcgagcga tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt
    48901 tgctcttttt ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta
    48961 gcgtgatggg actttctttc atcctaccag ctgttttagg aactgtactg gatgctcata
    49021 caaattcgtt gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat
    49081 gggctccact gacatgggta atcgtaggcg aaatattccc actagctgtc cgcgggcgag
    49141 catctggcct agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc
    49201 ctgtgatgac tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct
    49261 ttttaggggt tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg
    49321 aaatcgaaca aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt
    49381 aaagaggttg tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca
    49441 gtcacaagga acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga
    49501 ccagttggta ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag
    49561 cgatgacaaa agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt
    49621 aaaaacccgt ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt
    49681 catagagaat atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg
    49741 catcttgtcc caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat
    49801 ggcttaaaga agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat
    49861 tttctgtcaa cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat
    49921 ctaaagcttg ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt
    49981 ccgtggtgaa agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc
    50041 tgataaaacc tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca
    50101 gacgctgatg tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat
    50161 ctaaacaaat ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa
    50221 tgccttgttc tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg
    50281 taggctcaac gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct
    50341 ccaattcgtg atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt
    50401 cattttcgat tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga
    50461 caccgatcgt ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat
    50521 atgtgcccga ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt
    50581 gccgaatcgt atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg
    50641 ttgtatttac ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt
    50701 gatattttgc catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct
    50761 gcaaggttta ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt
    50821 tcaccaaatt ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga
    50881 ttaggattgt ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa
    50941 atatcaaagc acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta
    51001 ttttttttag aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa
    51061 agattcttgt gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta
    51121 ccagaatacg taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact
    51181 gaaaaacccc gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt
    51241 aacgtccaag gattatccat ttcggataga taaagattag aattcccagt aatttcagga
    51301 tcttgttggg cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa
    51361 ctgaaagttt cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga
    51421 tctatatgct gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt
    51481 ttatgattgc ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt
    51541 gcccagatca attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca
    51601 gcatgttcta attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga
    51661 accgatcctg taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt
    51721 tgccgaacga taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct
    51781 ttattctaat tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt
    51841 ctattgaaaa atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt
    51901 ccaacagatc gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat
    51961 gttcaatcgt tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat
    52021 tgccgttatc tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat
    52081 aaacagggca aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt
    52141 gcatcacata ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag
    52201 gtattgtgtt tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa
    52261 tagccagccc taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga
    52321 tgggactacc gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt
    52381 catcaaacgc tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag
    52441 tagcaacgat ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg
    52501 tatcatcacg ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat
    52561 agttatgcaa agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc
    52621 attcagggta cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat
    52681 catccactaa cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac
    52741 tcttagcccc cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc
    52801 caaaaggctt tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa
    52861 aattacaata ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg
    52921 tgaatatcgg tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag
    52981 aatttggttc tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac
    53041 ctacagtatc cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac
    53101 ctaattctaa ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg
    53161 tttcatggcc cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg
    53221 agccaaacag ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca
    53281 cttcttcgtc caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa
    53341 gtttccatac gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta
    53401 caaatcctaa taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc
    53461 atgcactttg ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata
    53521 tccaggataa aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta
    53581 cgattgtctg ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac
    53641 taaggaacaa gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat
    53701 ttcctgatcc ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca
    53761 cgatagtaat aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag
    53821 aaaccacaat caactgtcca taaagtttag tctcaactat tttcatcttc ctcactcttt
    53881 cattccagac atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag
    53941 taatggcagc attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg
    54001 ccctgtaaat aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat
    54061 caatggataa agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag
    54121 agtaggtttg ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc
    54181 aatgcgtgct gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa
    54241 gataccaaat gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc
    54301 aagcaaattg acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt
    54361 cagcaaaacg aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata
    54421 ccccgcaaga gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt
    54481 atttttgatg taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt
    54541 atagctatct gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga
    54601 agaaataatc agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat
    54661 cgttcgaatg atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga
    54721 gctttctggt gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt
    54781 acacattagt ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg
    54841 atgcatagcc caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg
    54901 tttctgtctt aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca
    54961 tcgaaccaat cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg
    55021 taatgttaaa aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag
    55081 agggagagat tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc
    55141 aaacagtcat gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga
    55201 tcggcaagcc gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca
    55261 tagaaaaaat aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac
    55321 gacaaaaacg attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga
    55381 taatttgtgt tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg
    55441 ctcgttcatc actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa
    55501 agaaaatatt caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa
    55561 ataataataa aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat
    55621 tccaatattt ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata
    55681 caatcttggt ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat
    55741 aacacctatc tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta
    55801 tcctgtataa agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt
    55861 tcatgagtgt ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat
    55921 cgttattgat cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt
    55981 gtttcgataa ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat
    56041 ccaagtaggc aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc
    56101 aatatttcaa aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg
    56161 catatcctac gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg
    56221 tagtcacatt ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga
    56281 tttcaatggc tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt
    56341 ctggaccagt cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct
    56401 caggagacgc aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta
    56461 acatccagtt atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt
    56521 tactactatc agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt
    56581 gttcagtatc taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt
    56641 gtacctgcat ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt
    56701 ctttaaaatc agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg
    56761 ctccgttttc tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt
    56821 ttttagaaga aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca
    56881 tttcaatttt gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata
    56941 tttcaccatc agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat
    57001 cagattctgc tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca
    57061 aagagccaat taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga
    57121 catctgacca cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct
    57181 tttacatttg ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt
    57241 gtatcaaatc cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag
    57301 tgaacagtta gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg
    57361 tcatttatta cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag
    57421 tttgctgaac gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata
    57481 aatggataat agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca
    57541 ataacattta ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa
    57601 gtgattaata gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa
    57661 ggaggagcaa tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata
    57721 ttccattcat caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta
    57781 tcgcacatcg ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa
    57841 tgagaaagat catttttttg gttgccataa tattgatgca gagacaaata atctatttga
    57901 tcgtacgctt gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc
    57961 gaacttccgc ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt
    58021 tcctctgcta accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc
    58081 atttcattcc ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc
    58141 cttaaatcac tccaataagt tcctttagga aaattacaat attcgactaa attacgagct
    58201 gcatctactc cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga
    58261 caccagttat aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa
    58321 tccaaccttc taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac
    58381 acaaaatttc caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg
    58441 acgtcttggc gaaatccact tttattagct gatggatgat ctggctgata aatcccttca
    58501 tatactgctc gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct
    58561 acacgcttga cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata
    58621 catgaataat acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt
    58681 ttttatcgat tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa
    58741 cttttattaa caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca
    58801 actacaaata aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat
    58861 caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact
    58921 tggagtgagc agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat
    58981 caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat
    59041 cagttttcca gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg
    59101 tcactataca aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat
    59161 cgggaaagtg gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct
    59221 ctggtttacc agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac
    59281 attggagatg ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa
    59341 ttggccttca gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc
    59401 tggagatttt gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa
    59461 tcaatacggc ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga
    59521 acccttgtca aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg
    59581 aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt
    59641 cggtaattac gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct
    59701 attttataaa acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt
    59761 atttccagaa atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc
    59821 accatgcttc gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat
    59881 caatttcatt atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt
    59941 caagatctgc agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca
    60001 attgtttccc ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac
    60061 caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc
    60121 ctgctctttc tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc
    60181 ctttgttaaa atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa
    60241 cagctggttt gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt
    60301 ttgcatcttc ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt
    60361 tttcatcttt tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta
    60421 aagtagaatc ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt
    60481 tcaaatatgc tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg
    60541 gtttgacacc taattcattg ataatttctg tctgcaggct catttattct cctcctatgc
    60601 ttctgtatga agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt
    60661 ccagcattca gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc
    60721 ttcccatagt gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa
    60781 ttcgtaaact ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc
    60841 tcgaacagtt ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc
    60901 tcgagggtct tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg
    60961 agtgatgcgc cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa
    61021 cttgatcgta tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc
    61081 accaagggct ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat
    61141 tttagctttt tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata
    61201 gatttttgct tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata
    61261 ggccatatct ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt
    61321 cgccacgcga atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa
    61381 cacgcagtct ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga
    61441 ttggactaac gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg
    61501 tacattgctt gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc
    61561 atccagttct tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga
    61621 tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc
    61681 tgtttctact aattggcagt gcgctaaagg cccttccact tggattaatg gttcatttgc
    61741 aaaaactagt tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa
    61801 atactccaaa aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc
    61861 attgaacgtc agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta
    61921 tccgtggtta aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg
    61981 acctaattcc cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa
    62041 actgtcgtct gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc
    62101 ctacccatta taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag
    62161 cacatgtttt acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc
    62221 ttattggtat ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac
    62281 gaagaaatta gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca
    62341 atcgtagccg ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa
    62401 tcaatggcaa aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa
    62461 agaaacggat ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat
    62521 ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag
    62581 taagaaagca gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc
    62641 ttgtttttta ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg
    62701 ttattaaata gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg
    62761 tttgaattgt attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc
    62821 ttctcgtgtc atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt
    62881 tttcttcgta ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg
    62941 tgttcgatct aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc
    63001 cactacttta ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag
    63061 cttcatctca ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc
    63121 atctttccta gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc
    63181 ttttatcact taatagtata acataatttc gtgagaaaaa aatacaattt tttcttgacc
    63241 atctcgttac gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca
    63301 ataaagaaaa tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag
    63361 attggactgc taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa
    63421 gaagtcgatc gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat
    63481 atccaagagg tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag
    63541 caaatgttgc tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca
    63601 cttgatatgt acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa
    63661 caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga
    63721 aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa
    63781 gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca
    63841 gaagatgaaa gcttagcaga ccgtatcttc caagctcata agaagtggtt ctcttacagc
    63901 tggcctgaat atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa
    63961 cggttcactg catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa
    64021 attatccaaa aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga
    64081 cctaataaca aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct
    64141 ccattctttc acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat
    64201 aaggtatttt cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc
    64261 aagcttccag atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat
    64321 cgcgtccaat caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa
    64381 ccatatccga atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt
    64441 gttttccttt ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa
    64501 aaagttgatg acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc
    64561 ccaacgtgat gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa
    64621 ttgattgttc tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa
    64681 aaactagagg tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg
    64741 taatgatcaa tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt
    64801 ctttatgata gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa
    64861 tgatcccact gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa
    64921 tgcttttcgt ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt
    64981 ctttcatgac cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga
    65041 ttacacgtga aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg
    65101 ccactgctgc ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg
    65161 accgcattca aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc
    65221 ccaatatata tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt
    65281 cctattcaac ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc
    65341 ataaaaataa ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc
    65401 tccttttgat actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg
    65461 ccaaagaaaa gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata
    65521 cattataatg atactgtaaa tatatcttta ttattagtga atg
//
LOCUS       DAYYFD010000004        54574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000004 DAYYFD010000000
VERSION     DAYYFD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.004"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..312
                     /locus_tag="ACHA25_000228"
     CDS             <1..312
                     /locus_tag="ACHA25_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HFM8761565.1"
                     /translation="EEFSTEEAGSMVWDDTFWIMLLSHDFYKKFIKDKEIADNKKTSG
                     ALISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMK
                     M"
     gene            549..1091
                     /locus_tag="ACHA25_000229"
     CDS             549..1091
                     /locus_tag="ACHA25_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8761566.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1174..1725)
                     /gene="lepB"
                     /locus_tag="ACHA25_000230"
     CDS             complement(1174..1725)
                     /gene="lepB"
                     /locus_tag="ACHA25_000230"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8761567.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1877..2104)
                     /locus_tag="ACHA25_000231"
     CDS             complement(1877..2104)
                     /locus_tag="ACHA25_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8761568.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2101..2619)
                     /gene="msrA"
                     /locus_tag="ACHA25_000232"
     CDS             complement(2101..2619)
                     /gene="msrA"
                     /locus_tag="ACHA25_000232"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8761569.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2639..3352)
                     /locus_tag="ACHA25_000233"
     CDS             complement(2639..3352)
                     /locus_tag="ACHA25_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8761570.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3355..4251)
                     /locus_tag="ACHA25_000234"
     CDS             complement(3355..4251)
                     /locus_tag="ACHA25_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8761571.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4409..5251)
                     /locus_tag="ACHA25_000235"
     CDS             complement(4409..5251)
                     /locus_tag="ACHA25_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8761572.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5383..6036
                     /locus_tag="ACHA25_000236"
     CDS             5383..6036
                     /locus_tag="ACHA25_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8761573.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6099..6626)
                     /locus_tag="ACHA25_000237"
     CDS             complement(6099..6626)
                     /locus_tag="ACHA25_000237"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8761574.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6646..7593)
                     /locus_tag="ACHA25_000238"
     CDS             complement(6646..7593)
                     /locus_tag="ACHA25_000238"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8761575.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKRPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7605..9488)
                     /locus_tag="ACHA25_000239"
     CDS             complement(7605..9488)
                     /locus_tag="ACHA25_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8761576.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(9651..11729)
                     /locus_tag="ACHA25_000240"
     CDS             complement(9651..11729)
                     /locus_tag="ACHA25_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8761577.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11742..12017)
                     /locus_tag="ACHA25_000241"
     CDS             complement(11742..12017)
                     /locus_tag="ACHA25_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761578.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12379..12834)
                     /locus_tag="ACHA25_000242"
     CDS             complement(12379..12834)
                     /locus_tag="ACHA25_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761579.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(12988..14196)
                     /locus_tag="ACHA25_000243"
     CDS             complement(12988..14196)
                     /locus_tag="ACHA25_000243"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8761580.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14308..14640)
                     /locus_tag="ACHA25_000244"
     CDS             complement(14308..14640)
                     /locus_tag="ACHA25_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8761581.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14788..15663
                     /locus_tag="ACHA25_000245"
     CDS             14788..15663
                     /locus_tag="ACHA25_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8761582.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15745..16326)
                     /locus_tag="ACHA25_000246"
     CDS             complement(15745..16326)
                     /locus_tag="ACHA25_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8761583.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16332..17591)
                     /locus_tag="ACHA25_000247"
     CDS             complement(16332..17591)
                     /locus_tag="ACHA25_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8761584.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17593..18639)
                     /locus_tag="ACHA25_000248"
     CDS             complement(17593..18639)
                     /locus_tag="ACHA25_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761585.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18874..19149)
                     /locus_tag="ACHA25_000249"
     CDS             complement(18874..19149)
                     /locus_tag="ACHA25_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8761586.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19392..20702)
                     /gene="der"
                     /locus_tag="ACHA25_000250"
     CDS             complement(19392..20702)
                     /gene="der"
                     /locus_tag="ACHA25_000250"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8761587.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(20884..22113)
                     /gene="rpsA"
                     /locus_tag="ACHA25_000251"
     CDS             complement(20884..22113)
                     /gene="rpsA"
                     /locus_tag="ACHA25_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8761588.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22248..22928)
                     /gene="cmk"
                     /locus_tag="ACHA25_000252"
     CDS             complement(22248..22928)
                     /gene="cmk"
                     /locus_tag="ACHA25_000252"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8761589.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(22977..23612)
                     /locus_tag="ACHA25_000253"
     CDS             complement(22977..23612)
                     /locus_tag="ACHA25_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8761590.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23678..25081)
                     /locus_tag="ACHA25_000254"
     CDS             complement(23678..25081)
                     /locus_tag="ACHA25_000254"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8761591.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(25068..26099)
                     /locus_tag="ACHA25_000255"
     CDS             complement(25068..26099)
                     /locus_tag="ACHA25_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8761592.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26130..26351
                     /locus_tag="ACHA25_000256"
     CDS             26130..26351
                     /locus_tag="ACHA25_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8761593.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26516..27253)
                     /locus_tag="ACHA25_000257"
     CDS             complement(26516..27253)
                     /locus_tag="ACHA25_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8761594.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27406..28746)
                     /locus_tag="ACHA25_000258"
     CDS             complement(27406..28746)
                     /locus_tag="ACHA25_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8761595.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(28983..30506)
                     /locus_tag="ACHA25_000259"
     CDS             complement(28983..30506)
                     /locus_tag="ACHA25_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8761596.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(30884..31495)
                     /locus_tag="ACHA25_000260"
     CDS             complement(30884..31495)
                     /locus_tag="ACHA25_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8761597.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31618..31743)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31825..32541)
                     /locus_tag="ACHA25_000261"
     CDS             complement(31825..32541)
                     /locus_tag="ACHA25_000261"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8761598.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32547..33116)
                     /gene="scpB"
                     /locus_tag="ACHA25_000262"
     CDS             complement(32547..33116)
                     /gene="scpB"
                     /locus_tag="ACHA25_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8761599.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33100..33870)
                     /locus_tag="ACHA25_000263"
     CDS             complement(33100..33870)
                     /locus_tag="ACHA25_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8761600.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(33867..34238)
                     /locus_tag="ACHA25_000264"
     CDS             complement(33867..34238)
                     /locus_tag="ACHA25_000264"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8761601.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34257..35144)
                     /gene="xerD"
                     /locus_tag="ACHA25_000265"
     CDS             complement(34257..35144)
                     /gene="xerD"
                     /locus_tag="ACHA25_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8761602.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35240..35713)
                     /locus_tag="ACHA25_000266"
     CDS             complement(35240..35713)
                     /locus_tag="ACHA25_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8761603.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(35818..36681)
                     /locus_tag="ACHA25_000267"
     CDS             complement(35818..36681)
                     /locus_tag="ACHA25_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8761604.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(36877..38976)
                     /locus_tag="ACHA25_000268"
     CDS             complement(36877..38976)
                     /locus_tag="ACHA25_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8761605.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(39196..40305)
                     /gene="rpoD"
                     /locus_tag="ACHA25_000269"
     CDS             complement(39196..40305)
                     /gene="rpoD"
                     /locus_tag="ACHA25_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8761606.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(40329..42170)
                     /gene="dnaG"
                     /locus_tag="ACHA25_000270"
     CDS             complement(40329..42170)
                     /gene="dnaG"
                     /locus_tag="ACHA25_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8761607.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(42352..44667)
                     /locus_tag="ACHA25_000271"
     CDS             complement(42352..44667)
                     /locus_tag="ACHA25_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8761608.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(44852..45712)
                     /locus_tag="ACHA25_000272"
     CDS             complement(44852..45712)
                     /locus_tag="ACHA25_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8761609.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(45771..46403)
                     /locus_tag="ACHA25_000273"
                     /pseudo
     CDS             complement(45771..46403)
                     /locus_tag="ACHA25_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            46570..47331
                     /locus_tag="ACHA25_000274"
     CDS             46570..47331
                     /locus_tag="ACHA25_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8761610.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            47357..49948
                     /gene="mprF"
                     /locus_tag="ACHA25_000275"
     CDS             47357..49948
                     /gene="mprF"
                     /locus_tag="ACHA25_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8761611.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(50136..51323)
                     /gene="tuf"
                     /locus_tag="ACHA25_000276"
     CDS             complement(50136..51323)
                     /gene="tuf"
                     /locus_tag="ACHA25_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8761612.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            51691..52047
                     /locus_tag="ACHA25_000277"
     CDS             51691..52047
                     /locus_tag="ACHA25_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8761613.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(52288..53292)
                     /locus_tag="ACHA25_000278"
     CDS             complement(52288..53292)
                     /locus_tag="ACHA25_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8761614.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            53458..54309
                     /locus_tag="ACHA25_000279"
     CDS             53458..54309
                     /locus_tag="ACHA25_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8761615.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            54326..54571
                     /locus_tag="ACHA25_000280"
     CDS             54326..54571
                     /locus_tag="ACHA25_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761616.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT        15274 a        11376 c         9367 g        18557 t
ORIGIN      
        1 gaagaatttt ctactgaaga agctggttca atggtatggg atgacacttt ttggataatg
       61 cttctctctc atgattttta taaaaaattt ataaaagata aagaaatagc tgataataaa
      121 aaaacaagtg gtgctttgat ttcgtttagt gttgaaaatg cggatgaagt aaaaaaattt
      181 gctcaaattg caaaagaaaa tggtggtact ttccacgaag tagatatggg aatgcctgaa
      241 gaagaaatgt tttcattaga agtccaagat ctagacggaa atacattaga acccgtatgg
      301 atgaagatgt aagcagataa ctaatctgac gttttttata acaaaaaaac aaaggcggat
      361 gatacgataa gtattctccg cctctatttt ttttattcgt acaatctctt ttttacagtt
      421 tataaaagca gtcttctcac ccttttttta attgttagat gccagtgtat gtaaatcacc
      481 aatgtgtcat gacacattgg tgattttatg gtatcctttt attattaact gacaaaggag
      541 agaatatttt gaacactttg attgcaaaag acgtttttag attgcccgaa atgaatgcaa
      601 gattgttgtc gacctctgct cttttattag cccttgatgt catcttatac aagatagcga
      661 ttggtcctgc ttattttcaa atcacattcg gatttttaag tatggcgctt atgggttatc
      721 tatatggacc gatctgggct ggtttgcttg aaatgttgtc gaacacttta aatttcacga
      781 tatttggttc aggaacgttt caagctgctt ttcttatcac ggctttcctt ggtggattta
      841 ttaatggctt atttttatat cataaaaaga taaacctgcc ttccgtcatc gctgcccaat
      901 tattgattat gatcccagtc agccttttta tcaattcttg gctgatgtct gttttgtttg
      961 gtgcagattt caaagtaatt tttctaggta gattcatcag gaatatgatc caaatcccta
     1021 ttcaaataat ggttgtttat tcatttatca aaactttaga ggctagaaaa gtgtttaaac
     1081 gagctgtttg attttacgta ctaaggtagt aaactgattt tggattaaac aataaagcgt
     1141 gcagtctcaa cagactgcac gctttattgt ttctcactgg atccatttta tttcatcaag
     1201 tggataatag acaaatcttg ctttacctag aatatcagtt ttattgatcg tgccaaacga
     1261 acgactatct tttgaaactc ttcgattatc tccaagtaca aaataattat cttccggaag
     1321 cttggattgt cccgttagct cttgtagtgt aaagtccgtc gtataggatg catttgcatg
     1381 atctgatttc aggtttttag ttagataagg ttccttaact actttcccat tgatcttcaa
     1441 ttgatcattt tggtagctga cattttctcc tggaagaccg attactcgtt tgatataaat
     1501 cgtgccatct gctaattgaa aaacaacaat atcaaatcgt ctgatctccg aaaatttttc
     1561 catcacaacc atatcacctt gcttcaacac gttttccatt gaattgcctt ccactggtac
     1621 cgggatgaat aaaaatcccc gcaacatgaa cgcaaggaat acagaaacaa gtaagtattt
     1681 gaatacaagc cagaagcgat taatatagcg ttgttttttt gtcaatctct atcaatccat
     1741 ccctcttttt ttcattcttt ctttattata agaaaaatat caaaaaaatg cgactagata
     1801 gctgaaataa aaataattct ttcaattagg ctattggaca aaaaactttt ttgatcataa
     1861 agtacaaaac tatctcttat atgtcatgat tattttctaa atatttttcc catgcttcgt
     1921 caaagatatc catggacggc aaatagtcga cattcagttc cagataatta gaaatttctt
     1981 cgtagctaga gctgtgctta ggaaattgga tgtctttacc ggcttctgta gcgaaaacag
     2041 ataaagcatc atgtgtgcca ccgcctcgca gtgtcattaa ataatgaaaa aagcttcgat
     2101 tcacccttcg ttcctccaat tttcttcgat aaaggcagcc cggcgtgcat ggtttctttt
     2161 aaaattttca ggatgctttt tataatactc ttgatggtct tcttctgcta gataaaatgg
     2221 ttgggctggt tcgatttttg taacgattgg ctgttcgaaa cgtccacttt cagctaactg
     2281 tttcttactt ttttcagcaa tgatccgctg ttcttctgtc aaataaaaga tcaccgggcg
     2341 ataattgtca ccgcgatcct cgaattgtcc gaaagcgtca gttggatcag tttgctgcca
     2401 gtaaagttct actaattgtt catatgaaat caatgttgga tcatagataa tctccactgc
     2461 ttctgtatgt ccagtcgttt ggctttttac ttgttcataa gtcggatctg gtacatgccc
     2521 tccagtatat cctgacatca ctgtatggat accaggaagc gtgtcgaaag gctggatcat
     2581 acaccagaag cagcctcctg caaatattgc tcgttccatt atgattcccc ctctaatact
     2641 actcatccga tgatttttta ttttcttttg tcttgctttc agtactgctg gtgctagttt
     2701 tgttggtatt tgagttgttt gaagcatcag aggtatcttt tggcaaatat agactaaacc
     2761 gaatgtcatc atttactaag tcgattttat ctgctttaat atacatcccg ttttgcattc
     2821 ggaattgatc caacctaagc atgatagtca gatcatctgt attgacttcg atccaatctg
     2881 gtaatttata gtttctcttg atcatattca tcacatcttt gattggcaaa ctcaatgttc
     2941 cgatagaaag acttctagct tttagttgga catttccgtt ttccatcaca tacggatcaa
     3001 aatataaata gaaatcgata ggaaaaccta gtacttcgaa agtaccattg agtagtgctt
     3061 cattttccaa ataaaattta tattcaatat cactatcttt ttgatactca ctaaggaaaa
     3121 agctgatgat ttgattgact ttttctttat ttgaattgat cgttaagaca ggatctcctt
     3181 gtctttccgt aagagctggt atttctggga gttctggttc ccgattagca aaaatacggc
     3241 tgcctaaaaa tacggtcgaa ccaagtacta ccccaagaag gataaggaag gcgaatttcc
     3301 agtaatttgt tttctttgtt ttgtttgaag atacgtcaga tcttgttttc atgtttattg
     3361 atcatcctcc ttctctaacc attggttttg tgtctcgatc agctgatctc tcactgcatt
     3421 tgccatcaat tgataaccta aattatttgg atggaaatga tcttcttcat agagcacatt
     3481 atttttgatg ccatttagat tttctgtcgt gctagttgtt tgatctgttc cttcttctgt
     3541 gacacctact tcctgattca atccttgata aagcaaatca ttgattggga taaagtagca
     3601 gttcggcatt ttttttgtca ccgtttctgt tcctttgttc caattgttaa caattgtctg
     3661 catgtcagtg atctcaggaa agttcaaata aaatggattg tagatgccta aaatgtaaat
     3721 aggtgcggtt ttattgaggg atcgaaattc ttccaatagt tctgtgacat tttcctgata
     3781 gtttttcagt ggctttttaa aggaattgac cgttaacccg aataaattat tttggataac
     3841 tttcatcaaa tcattaccgc caacagtcag tgtgataaaa tcagctgttt tgatattgtt
     3901 tcgaatttct tcttttgttt ttactcgctt taagatctgg tcacttcgtt caccatttac
     3961 accatagttt tctgtttcaa ccgcggtcaa attataacgg tcttttagat catttgcgac
     4021 aaggggaacg aatcctcctt gcttggtttg atctcctact ccttcagtca gcgaatctcc
     4081 gatagctatg tagtgaagtt tttctttttt atttggatct tctctcacag tagttactgg
     4141 tttcattagt gggccagctt tcggtataga agcagctaaa cctgcataga tgatgatcgc
     4201 aattaataat gggaacaaaa cgaggatcaa atgtcgtttc gttaatttca ttttttcacc
     4261 tcttgtattc gattggttct aatgttatca aaaattttat caaaaaggta gtctttatga
     4321 aaaaagaaaa caattatttt cctaatttta taaagaaaaa gagaataagc tgcaagcctt
     4381 tcaggctgag ggcttatcct cttttcgact aatctacata atacatgatt gcaaacgcgt
     4441 ctttaccagt atgcgtagcg ataaccggat tcgtatgcag tacagggata tgcatgtccg
     4501 ggaataattc ttgcaaaccc tctttgaatt cttgcgccaa ttcttttgca tccgcatgtg
     4561 aaataccgat ttgtcgcaca tttggcaatt ctttcagttc atttttcaaa ttattaaacc
     4621 atttatcgaa cgttttcttt cctcgcccct tagttacagg gattaattca gaatgggcga
     4681 aattcatcac cacgcgtatg ttcaaaacac tagacagcaa accagttgtt cggctgattc
     4741 ttccaccttt taccaagtta tctagtgtgg atattccgat gtataatttt gtattgtctt
     4801 gtactttttt gacttcttca aggattttct ctactgtttt accttctttt gctaattcag
     4861 ctgctcgaat tacctgaaaa gataatcctt ggtcagtggt atcactgtcg ataaccgtta
     4921 ctttcgtttt tgttagatta ctggcttgtc ttgctgcttc tactgttcca ctcaagcctt
     4981 ttgtcatatg gatcgaaatc acttcactgc cgtcttctcc tagctcatca tacatcgaga
     5041 tgaattctcc aattggtggt tggcttgttt tgggcagatt ttttgctttc gccatcattt
     5101 ccataaatct ttcgccgtct agttggtcat catcgggata tactgttccg tcgatcatga
     5161 tggatagcgg catgacacga atgcctaaag catcgcgtac atttttttcc atcgtacatg
     5221 aagaatctgt tacgattttt acatttgtca ttattttctc tctctctttc tttaacaaga
     5281 tgccttattc atgttaaact acatgtaaga caatttctat ttttagtata acagacaaat
     5341 aatgattttt cattaattac acctagtaaa ggagccgttt tcatggaaaa aacgaaattt
     5401 aatcgaacgt atgaaatatt aaatgaagtg tttaatgcag tcacccatgg aattgggact
     5461 ggattgagta tcgcgggact agtggttctt ttagtaaaag gtgcccactt gcattcctcc
     5521 ctccatattg tctcttatgc tatctatgga tcgatgatga ttcttttatt tttaacgtct
     5581 actttgtttc attcattgat ttttacaaaa gcaaaaaagg tgttccaagt attcgatcat
     5641 agttcgattt ttcttttgat tgcaggaagt tatactcctt tttgtttgct gagtatccga
     5701 ggatggctag gttggacttt atttgtatta atctggttgt ttgcgattct tggcattata
     5761 tataaagcgt tgacgttgca taaacgggag agagtatcaa aagtatctac gatcatctat
     5821 atttttatgg ggtggctctg tgtcgtggct gctgttcctc tttatcactc tcttggaatg
     5881 actggtgtta ttttgatggt agcaggcggg ctttcttaca cgataggcgc attcttttat
     5941 agcttgaatt ctatccgcta tatgcatgtt gtttggcatt tgtttgttat gttaggtgca
     6001 ggttttatgt acttctctgt tttattcttt acttaataaa aaaaataaag ctatggatgg
     6061 gacagctcta aataacagct agtctatcca tagctttttt atatcgtcaa ttcgcctgtc
     6121 ccgcttttta caggattctc attacgctga tacgtttcaa attgatgcgg ataacgattt
     6181 ttttcatcca tttctccttg actagtattg atcagtgacc aatcagacca gtcaacaggt
     6241 ggaaaataag catctccatc gaatacatga tggatgactg tacggtataa tatcgtacaa
     6301 aatggcagaa attcttgata aacagctgac cctccagcga tgaaaaagat accgtctgtt
     6361 tctttttcca gagcaagcac gtcttccaca ctatgcatga catgcacgcc ttctgcttta
     6421 taggaagaat ctctcgtcaa aacaatcgtc tgacgattcg gcaacggacg gctacccatc
     6481 ccttcgaatg tttttcgtcc catcactagt gtatttgctt ctgtcatttg tttgaagaat
     6541 ttcagatcat ttggcaaacg ccaaggaaga cggtcttctt ttccaatcaa tccgttttca
     6601 tcctgtgccc aaattgcagc taacattttc tcatcctttc tgattctaaa cagcgatagg
     6661 agctttgatc gccggatgtg ggtcatatcc ttctaattgg atatcttcca tctcaaaatc
     6721 aaatactgac tgcttttcct gattcaaaac taaagtcgga aatgcgcgta tttctcggct
     6781 caattgttct ttcatttgtt cgatatgatt gatataaaga tgagcatctc ctaatgtgtg
     6841 aacaaattcg ccaaccgcta aacctgtttc atgtgcaatt aggtgagtca gcaacgcata
     6901 acttgcaatg ttgaacggaa cgcctagaaa cacatcccca ctgcgttgat acaattggca
     6961 gcttagccgt ccctcttgaa cataaaattg aaacatcgta tgacaaggag gcaaagccat
     7021 gctaggaaca tcttccggat tccatgcaga aacgatcaat cttctagaat caggagtctt
     7081 tttgattgct tcgatcactt gtttgatttg atcgataaag ccaccgtctt ttgtttccca
     7141 gtgacgccat tgatagccgt agatgtgtcc aagatcgccg tatgtttcag caaaggaatc
     7201 atcggttagg atcttctcgc aaaaaagctt gctctgcttt ttatattctt cattgaattc
     7261 aggatctgtc aaacatctat ggccaaaatc agtcatatct ggtccattat agtctggcct
     7321 tttgatgtag cgttcaaatg cccattcatc ccaaatatgg tttttatttt gtaatagata
     7381 gcggatattc gtatctcctt ttaaaaacca aagcaattcg cttttgatca atccaaaagg
     7441 tactcgtttc gttgttaaca aaggaaaccc tttttgcaga tcaaaccgca tttggtatcc
     7501 gaataagctt aatgtgcctg tgcctgttct gtcttcttta cgatgccctt cttccaacaa
     7561 ttttcttcct aagtcaagat acgcttgttc catccttttt cctcctaatc ttctacccat
     7621 tcactcaagt attcccaccg tgccattttt tcttcaagtt gtgcttctgt ttcagatacg
     7681 tcgttttgca attcttgtaa tcgagtgaaa ttatctcctt gatgattcat ctcttcttgg
     7741 agcaatgaga ttttttcttc aagttctgca atctcatctt caatcgtttc ccattctttt
     7801 tgttccatgt aagataattt cttcttttca gcaggttcct tgctctgtat ttttggtttc
     7861 gctttttcag caggctgctg ggtctctttt tgcttcagta aataactgga catgtttccg
     7921 taataaagtt cttgttttcc gtttccttgg aatactaata acttatctgc taccttgtcc
     7981 aagaaataac ggtcatggga aacagtgatc acagcacctg gaaaacttct aaaataatct
     8041 tccaaaatcg tcaatgttgc tatgtccaaa tcattggtcg gttcatccaa taataaaaca
     8101 ttaggttgct gaatcagcag tttcaacaaa aataaccggc gtttttctcc gcctgaaagt
     8161 ttgcctatca gtgtcccatg cataaatctt ggaaataaaa atcgttcaag taattctgct
     8221 actccgattt gactgccatc tgctgttttt gcttcttcag cagcttcttg taagtaagca
     8281 atcatccgtt tatctggtgc catttcttca ttttcttgtg tataatacgc aagatgaacc
     8341 gtttcaccga tagacattgt accgctatcc aggggaattc tgccagctag aatattaagc
     8401 aatgtcgatt ttcccgcacc atttttacca gtaataccta gtctttcccg agattgaatc
     8461 aataaatcta aatgttctag cagtgtttgg tcatttatac ggtaactgcc atctttgatt
     8521 tccagtactt ttttccctaa acgttgtgtc gctaggttca gctctaaatc atcttcttgg
     8581 tttacttggt acaagttctc ctttagatct tcgaaacggt tgatccgtgc ttgctgcttg
     8641 gtgccacgtg cctgtacgcc agccctcatc caagctaatt cctgtttata aagccgcttg
     8701 cgcttttctt cttgttctac ttctacacga tcacgttctg ctttttctaa gacgtaagct
     8761 tcataatttc ctttatactc gtataatttt ccaaatgaaa gttcgaagat tcggtttgct
     8821 actctatcta gaaaataacg atcgtgggta accatcaata gtgcgccacg gtattgtttc
     8881 aaatagtttt ccaaccactc gatcgcttca taatctaaat ggtttgttgg ttcgtctagt
     8941 aatagtaaat cgggttcatc aatcaacact tgcgccagac taactcgttt tttctgacca
     9001 cctgataaag tactgatttt tttatctaat tcactgattc ctaatttttg caaaataatc
     9061 tttgcgttgg tatctgtagt ccaagcatct tctttattca tttgttcttc tgctttagca
     9121 taacgttttt gcacatcagg gtcttctccg ttttccccta gttcgatcaa tgcttcctca
     9181 tatgctcgaa cagtttggat caaggggctg tttccttgaa agactgcttg gagaacagtt
     9241 aattcatcgg aaaattcagc tgcttgagaa agataaccaa tacggtaatc agttggataa
     9301 aacactgttt gccgatcacc gtcgctgcta tcgataccgg caataatatt taagagactt
     9361 gttttacccg tcccgttcgt cccaatcaac ccgatacggt ctttttcgtg gaccaaaaaa
     9421 gaaatctggt caaacaaatc tttttcacca taagtttttt tgaggtcagt tacttttaat
     9481 tctttcaaca tgttccctcc ttatcttgga tattgtatca taaaaatagt gaacttcagg
     9541 aaaatttttt tggtatttgt tctttttgtg ttctcaaaaa aagaacagga ccgattcgat
     9601 cctgttctga tatgttttgt tttttataaa acttgaaaat ttttaccttt ttattttatt
     9661 ggactcgttt tttttgactt tgctcgaaac gcaaatcgga aagaaaagtt atcagatggt
     9721 ttgttcatcg tataagattc taacaaattg atattcctgc gatagctgtt cgcagaaaac
     9781 gtcgtgattt tttcagcaac ttcgtattca tcaatgattt ttctttcaag ataataacct
     9841 cttagcattt ttttcgtaaa cagactatct tgctggatca tccacgaaga aatcaaattc
     9901 cccgagttca tttttattcc ttcaagtttg cgttgctgga taaaaaattg aaccatcgtt
     9961 tcatcataaa tatcagtcaa gttatctaaa ctttcaataa tcaactgagt atttttttga
    10021 tatgtctctc tgatttcttg gatatcttca tcactaataa atgtctgacg atcagcatat
    10081 cttctttgcc aaaacatttt cggatggaga acgattcgca ttaacctacg gacaacaatg
    10141 aaccaaaagc cgataaaaga caaaatctgg cttgtgatag aatgttcgaa tcgagaaaga
    10201 aaacgggagt aaaaacgata accattagaa gaaagcttgc ccgaacgata actttcatcc
    10261 aatccatccc gttcgatcga caaaatcagt gcttgtattt cttgtacttc ttgcttttca
    10321 tcatcggtta aatcttccaa gtacagatca cgtatccgtt cttgataatt ttctatcact
    10381 gcttcagtgg ctaaaaattc ttttgtatta cgatcaattt cctttatttc ttttcgcaaa
    10441 acttcaacga cctcttctaa aatcatcaaa gcattcaaat ccgtgctttt cgcttcctct
    10501 gtttccgtca gcataggcaa gacaatgatc cctatgacca aagtcactaa gatgacaccc
    10561 gccgtcagaa acaaaagcaa agaccgctgg taaaacatca tattattgat tgaaaatggc
    10621 aaaataaaaa tcgtagccaa actgactgtt ccctttacac cgccaaatgt taagatcaag
    10681 atctcgttca actgtttttt gaacttttta ctgccatctt tgaatacata aaaaagactg
    10741 atagaaatga atctgatcac aaaaagcatg acagagatta atacgataat cgctaataaa
    10801 agaccgtttg gatataaacc gtctccccat actggcgaga acacttgcgt caattcgatt
    10861 cctaaaaata taaatactaa tgcattcagt gtaaaagcaa tagttgtcca tgtactgtgg
    10921 gacaagctcg acaattctgc atcaaacaca gagattttcc taaaaccaga agcttggaga
    10981 atacctgctg ccactgcagc aatgattcca gatacatcaa atgcctctgc caatacatag
    11041 gctagaaaag gaagcaaaag ttcgatcagc agataccctg tgacatcttg tgctgatgct
    11101 ttttcgatca aatgaattat tttttgtttg aaccagacaa gcaaaaagcc gatcaaagct
    11161 cccccaatac tagaaactac tagactcatc cccgcgttta ctgctgaaaa agatccggtg
    11221 atcagcgcac caagagcaaa ctggaaggca gtaacaccag atgcatcatt caaaagacct
    11281 tctccttcca aaatatgcat cgccttttta gggatattca ctcgtccaga aagagaactg
    11341 actgctaccg catctgtcgg acctaacgct gctccaaaag caaagcaggc agcaagtggg
    11401 acacttggca acaggaatga taacgtcgca cctaccccga caagggtcaa aatgactcct
    11461 ccaaatgcca agaacaaaat tgtcccgaaa tttttgagaa tcgaagaaat atctgctttt
    11521 tctccttctc taaataacaa tggtgcaata atcataacta agaacaattc aggttcaaaa
    11581 tcgattgatt gtccccactc tgtcagacct aataccactc caaggaaaat ttggatcagt
    11641 ggagctggaa tcattggcaa aatgcggcta gctatgttcg ataaggtgat cgttactgca
    11701 aatacaataa ctaactcagc aaattccatc gtccatcccc tttatttttt attttgtgta
    11761 agcattttta aaaattgttc ttttttcttt aaaattttgg tttcgactac ttcaacctgt
    11821 gtttggttcc cctctaattc acactgtaca cgttttgact ccaaacgaag cagatcatgt
    11881 ttcagacgtt ccaactgacg atacatcaat atatttcttt tacgcaactt acaaagtatt
    11941 tgacggcggt catccatcga aagggcgcca tagcgatcat gcaacagttt tttttgttgt
    12001 tcaaaactct ctctcatttt ccagatctcc tatacacaaa cttccatcat tatagcaaag
    12061 ttctttttgg atgaaaaata tttcattgtt tcatcgttta ttgctagtta gaaatttcca
    12121 taaatttttc aacgcctcat tctattccaa atagtatcag atatcaagta aaacttaaga
    12181 aagataaaag tttctgacaa aaggatgttt actttcccat tcctgccttt cttgttcttt
    12241 tatcacaaca aaaaaatggg gctgcgacaa aaatcttgtc acagtccctt attctgaata
    12301 aacggtgtgg aagagacagc ctatcagtag taataagaca catctctttc acagcctcat
    12361 ttttgcgtaa aaatcgattt attcgaaaaa ttccccgtat acatcttcta cacgttgatc
    12421 tggattgatc acgatataca agttacctgt tttttcgctg atttttgttg attgatattg
    12481 attgtctaat ccttctgttc ctttatctag atatgggaag tacacaagat caggtgtgcc
    12541 atttcggtaa aggatttcca ttcgctcgat atcttcgtat ttcaatgcgc gattaaacat
    12601 gcccatttcc agtccgccta ggttgatatc atgtgtcgca actgcgtccc cttcatggaa
    12661 gatttcgatt ttaaatccag cacacggatg gatttcaaca aattcactgc cattgattcg
    12721 tccaaaactt gttgtgattt gtttgatcca aagatcgccg atatgacggc ggttgatcgt
    12781 ccaggtttct ccatttctaa aataaaattt tagcgctgtc atttctttac tcattaaaaa
    12841 gactccttta tgatgtaatt tattcttgta ctacctcagt ttagcacaaa tttatgtaag
    12901 tggtcacatc ttatgttttt tcttctaatg acgtatctgc ccactttaag tagccaacaa
    12961 agctgagcag atacagtcta gtgtttttta aatcaatttt tctttcgata aaaaatccaa
    13021 aacctgtttt ttatcatttt ttattctttg ttctacgacg gctttttcag ctaattgcaa
    13081 agcttcacta acccacttac caggtttttt tccagtttct tctagcaaga ttttaccatc
    13141 tactgctaat tcgcttaatg acttgatagg taaacgatca taggattctc ctacggcatc
    13201 catctgaggt gtttcgtcaa aatagatcat caattcttct acttgcagtg catgtttttt
    13261 gcctaagaag tacaattcga atggttccaa agctccagtt tctcgttgtc gtagtccttg
    13321 gacaagtgct tgaacgtctt taatcatttg gttagaacac ttccacgatt tcaaaaagga
    13381 acgaatctca ttttctttta gatccagttg gctgatcaat aatgcccatg cttggctctc
    13441 ttctttcaat gggacggatg gtaattcagc aaaacgtaaa agtgcttcgc cttcttcttt
    13501 caaaccggga cagcaaatgt agcactctgt ttcaataaat gcatggattc ccgcggcccg
    13561 ttcatttccc aagagcatct tgatgaattc cacatttata cgttctacag atattttacc
    13621 taatagctcg tggttttctt tgatggcgta gaaggtttct tcctctaatt tgaaccctag
    13681 ttgactgaca aaacgcaacc cgcgcatcat tcgtaaggca tcttcatgga accgctcatg
    13741 aggattgcct acagctcgca agatctgttc ttctagatct tgcagaccat cgaacaaatc
    13801 gatgatttct ccatcttctt tcaaagcaaa cgcattgatc gtaaaatctc ttcttttcaa
    13861 atcttctgcc aacgaacgaa cgaattcgac atgatctggt ctacggtagt cctgataagt
    13921 tgattccgtc cgaaatgtgg tgatctcata ttgttcatca ttttctaata ccaacacagt
    13981 tccatgttcg atcccaatat ctatcgtttt tggaaaaagc tgtttgattt cttctggaaa
    14041 tgcgcttgtt gcgatatcca catcatggat tggctggttc agcaagatat cacgaacact
    14101 tccacctaca aaataagctt catatcctgc attctctaat tttttcagaa ctggaattgc
    14161 cttttggtat tccagaggta attgttttaa tttcattttt ccaacctttc ctgcgtgttt
    14221 cttgtcctga gataaagact cagttgcatg gtgactgttt gacaagtccg tttcagagtc
    14281 ttcaaggcca ggaccacttc atcttgttta ctcgtcctta cgctcaaagc gattatggtc
    14341 tctttgattg aatttttcca tgtttttttc aaaaacttca gttaagtcga tattcaatga
    14401 attagccatg atcatcgtaa cgaataaaac atcaccaagt tcttctgcta ccgagttaac
    14461 tggttcagaa gcctttttgc ttttttctcc ataagtgtgg ttgacttctc ttgccaattc
    14521 acctacttct tcagttaatc gagccatctg agctaatgga gagaaatacc ccaccttgaa
    14581 ttgctggata tattcatcca cttcctgctg catactgtat aaagaacgct ttttatccat
    14641 ctttttccct ccaaaactat cttaccaaaa ctttaacgca aatcctagat aagagcaatt
    14701 gagaaaatcg aatttccatg ctacatttag gaaggaattt ctaaatggaa attctgtgaa
    14761 aatacataat tgaaagctgg aattcatatg gtcaaacgaa aaacagtaaa agaagttctt
    14821 tttatcatta taggaacaag tatttatgca tttggtcttg tttacttgaa catcgctaat
    14881 catttagcag aagggggagt ctcagggatc accttgatct taagagcttt atttggtatt
    14941 gatccagctt atacgacttt attaatcaac atcccattga ttttactagg aggaaagatc
    15001 ctcggtaaac gttccttggc ctatactgtt ctaggtacac tttctttgtc attcttttta
    15061 tggatctggc aaagggttcc actagaaatc aatttacagc atgatctact gatcgtttct
    15121 ctacttgcgg gtttagttgc aggtgtagga agcgggatcg tctaccgcat gggtggaaca
    15181 acaggcggca gtgatattat cgctcggatt ttagaacaaa attacggcat cactatgggg
    15241 cgttccctat tggcatttga tatcatcgtc ttacttgctt cattaaccta tatcgacttg
    15301 aaacgtatga tgtatacgtt gattgtttcg tatgttttca gtcgtgtgat tgattcgatc
    15361 ttagatggcg gatattctgc aaaaggtcta cttgtcgtct ccaacaaaag cgaagagatt
    15421 gcaccactac tgatgaccgg tctgcaaaga ggtgtgactt tcctttctgg agaaggcgca
    15481 ttctctggta attcaaaaaa aatcatctat atggtcgtta gctctagtga attaactgaa
    15541 gctaagagga tcgtccacga gattgacgaa aaagctttcc tttcaatcat caatgtgcat
    15601 gaagtggaag ggcaaggctt tacttatttg aagccacaga caagattact gaaaaaacta
    15661 taagaaaaaa gacaaagata ggctatgagg aaagattcca catccagctg acctttgtct
    15721 tttttagtac tatactattt ttaactaatt gttatgttca cttacccggt tacttttctt
    15781 ttgtgtctgc aaaaggatct ttcggttaac ttcatcagat aatttcaaaa aagcatccct
    15841 atctccatct tctagtgctt gatcgatttg agcataaagc atttttagtt ttgcctcttc
    15901 ttctttttct tgaaaatatc gttcaattaa ttcagtatct tcttcactga ctgaagaatc
    15961 ccaagaagaa aatggattgt cttctaagat agatagatat tctcttgtct gccaagcatt
    16021 ttcgaatata cattccacat ataaaggctt tttccaattc aaacggattt catgaaaaat
    16081 ctggtctgtg tccgtaaatt cttttccctg caaaaacaat ttcatgggtt ccccttcaac
    16141 agatagatca ctgatctgca gtcctcgatc cgcaaaattc gccccttcaa caaagtggac
    16201 attgcttaaa atcgcttcat gattggcaag ataattcaag atccaaaaaa cttctcttcg
    16261 gctaaaagat acatgattga ccatccacgt taaaaaattc ttcttttccc gtacatcaat
    16321 tagcattttt ctcacctctc tgttaggtca tctaataatg acagtgcctc catatctcct
    16381 ggttcaagtg ttaaataata agtcaataat tcttgcgctc ttttcagctg cccttcttct
    16441 cttaagaaaa aggcatactc tttcaagaaa tctggttcat gtgatagttc atgataagcc
    16501 tgttcatagt gtacagctgc aacggtaaag tcttctaatt cattataagc atgggctagg
    16561 ttccattctg cataaggatt agcagtttct tccatctgat tgatcacttg tatcacatct
    16621 tcatatcttt catcatccaa gtaaagatta cttaaagtaa gtaatgtttc atcttgtttg
    16681 tctcctactt ctaaagcttt cagtaaaagt tcttctgctc gctgtctatc atgcagacgg
    16741 aaagcatttt ccgaggccag gtgataaagt tcgacttggt aaggattttc tttgattcct
    16801 tcttctaaca ctgtttgtgc ttcttcaatc atttcttctt cttgaagagc ttgtcctaga
    16861 tatagatata acgattgata atgcggatta acttctcgaa gttcttccag atagttgatt
    16921 gcctgctgat tttctttcaa ttgaagattc gtaaaagcta gctggaacag tcgatcatct
    16981 gtcctttctt ctttcagtgc ttcttctaaa ggtacgatag ctgcttcgaa ttctccttgc
    17041 atacttaagg attgtcctaa acgttcctgc atcgagattc ctgaaatttc atttatcccg
    17101 cttgccaaca atgtttcata aaccactgct gcctctttga aacgatcaac tgaaaaatac
    17161 aattcagcta gcgcaaactg gatcaatggt tcatcaggaa gcaacccagc tgcttctttc
    17221 aattttgctt cactcacttc agggattccg ataacttggt acaaatcagc tatcgccaat
    17281 aagctttgcg gatagtaatc actttgctta tccacttttt ccaggtattc aaaagctaag
    17341 tcgatctcat tatcttcgat tgcaatttct gctaacggga tgttcagtcc gtcattttct
    17401 gggtattggt tgatcagttg ttcaaaaatc tgttttgctt cgatcaaaaa gccaatagcc
    17461 agcaattctt cgcctagttc agctaatata tctgctgggt cttcttttaa tgctttttct
    17521 aaaaagattt gtgcttgtga caagtcttca tctgataaag cttgcaacat tttttcgcta
    17581 tttgtattca tttcaattcc ctcttatctc actgacgatt tgttgaaata ccgtcattgt
    17641 tctcaataat tctttttcgt ttggatgttg gtatccgtct attataccta ttttttctaa
    17701 aacaagtatt tttttgcttt tttctgccaa aaaaagaact ttttccaaat actccatctt
    17761 tgaaatctgt tccagccagt ggattggata gccaagcaag tccagccaca caaaaaagtt
    17821 ttttagttgg aaatcaaaag gatatgcttg gagattccac acgaaatgaa acatcattcc
    17881 taaaaatttt ttcatactag cagaaagtag atggccattt tcggtcagat aaaaagcttg
    17941 ttcaaacagt ttaccataat ttgacaggtt ttctgcctct aattgataaa aatagatcat
    18001 ttcttcgatc atcccggcaa acggccgagt cgttaattgc tttttatttg gatagttttt
    18061 atacagattt tgcaaaaaac gatgatcaca aatcattccg caacagataa aagtcaaaaa
    18121 gtcaatctgc tttccgtcca cctgccgctc gctgattgtt tgatcataaa cgatgcgttc
    18181 tggcagattg atggtttgca gcactacacg atttggtagc tgcaaaatcg tccgctgcgg
    18241 tgttaatgct cggcttagag aacggatcga tacagggatc atccatagtg atgaatcaag
    18301 ggttgtatgt ttttgaaaaa aacctgccag atccatgatc ccttcatttc caaatccgat
    18361 gataaacatg gattgatttt cgggatagcg tttgacgaaa tcaagcaggt tctttaattc
    18421 tgttaaatga ttgcattgag tgttggcaca aatataccag tcattgtttg cttgatttgc
    18481 aaataacggg ttgatcttat ctgcaaacaa atcataatag cgctgattcg ttaaaaataa
    18541 gattggctga tcaatggaaa gttctcttag ctgactgact agcgtttctc cgtaaaacac
    18601 tttcgtcaac gcatcttcta cttgataatc aagttccata cgcatccctc ctcttcttat
    18661 ttttatttta ccattagtaa gcaaattcta gaaaagataa gagcaaagct cacctcataa
    18721 cttgcaaata aataaaaatt aaataaaata catgtgtttt ctatgtatga catacggatt
    18781 cggaatctct gtctcttatt tattcctttt ctgctaggaa ataaaaagaa aagcacttgt
    18841 agccctagag ccacaagcgc tttttcaact gtcttattta acagcgtctt ttaatgcttt
    18901 acctggtttg aatgcaggta ctttgctagc tggaatttcg atttcttcac cagtttgtgg
    18961 gttacgacct ttgcgagctg cgcgttcgcg aacttcaaag ttaccaaaac cgattaattg
    19021 aactttttcg ccgttagcta aagcatcttg aatagttgaa aatacagcat ctactgctgc
    19081 agttgcatct tttttagtta aatcagtagc tgaagcaact ttttcgatta attctgcttt
    19141 gtttgccatg gatagattca cctcctccac aagagatatg atagaaatat catttagtta
    19201 aaactttctg agtggtccag aagtttaaaa agaaatattg attaacttca atattctgtt
    19261 atcacgatat catatgaacc gcttaatagc aaggttttta ccaattttca catgcttttt
    19321 ttagtcatag aaacgtaaca aacgaaactg tctttttctt ttgaaaaaaa aacgtcgata
    19381 tggtaaaatg cctattttct cctacgaggg ataattcgga tggacgtccc ttcaaaagta
    19441 aatgcttttc tgatttgatt ttctaagaaa cgttcataag aaaaatgcat caattcctct
    19501 tcattgacaa aaacgacaaa tgtaggtggt ttgacagcga cttgtgtagc ataaaaaatc
    19561 ttcaggcgtt ttcctttatc tgttggtgtt ggattgattg ctacagcatc cataatgata
    19621 tcattcaaca cagccgaaga gatacgaaga ttctggttca tacttacttg ttcgatcatt
    19681 tcaggcagac gttccaaccg ttgtttagtc actgcagaaa caaaaacgat tggagcgtaa
    19741 tcaagatacc ggaattcatc acgaatctct tgttcgaaat cacgcattgt attggtttct
    19801 ttctttacaa gatcccattt attcacgacg ataataattc cacgaccagc ttcatgggcg
    19861 taacccgcta ctcgcttgtc ttgttcccga atcccttctt cagcattcag taccatcaaa
    19921 acgatatcag aacggtcaat ggcacgcatt gcacgcatca cgctgtattt ttcagtattt
    19981 tcgtatactt ttccgcgttt acgcatacca gctgtatcta tcattaagaa tttctgacca
    20041 ctttcagaaa cgaagtgggt gtcgattgcg tctcgggtcg ttccttcaat atccgaaaca
    20101 attacacgtt cttctcctaa aatcgcattg atcaaagagg atttaccaac atttgggcga
    20161 ccaatcaaac taaacttaat cgtgtcgtcg tcctcttctt cagtctcttc agagaaatac
    20221 ttcactgctt catctaaaat atcaccaatc cctagtccat ggcttcctga aacaggataa
    20281 ggatcgccta gccctaagga ataaaattca tagatatcgt ttctcatttc tggattatct
    20341 actttattga ctgctaaaat cactggttta ttacttctgt ataaaatttt cgcaaccatc
    20401 tcatctgcgt ctgtcacgcc ttcgcgtccg ctgactacac aaatgattac atctgcttct
    20461 tcaatcgcga tttctgcttg atgcttgatc tgatccataa aaggttcatc gccaagatca
    20521 attccgcctg tgtcgatcac gctgaattct cgacctaacc attcaccttt tgcataaata
    20581 cgatcacgag ttactccagg agtatcttcg acaatcgaga tacgctcacc agcgatgcgg
    20641 ttaaaaatcg tagacttacc gacgttcggg cggccaacga ttgcaattgt tggatttgcc
    20701 atattatttc ctcctcacaa gtttccaatc tcacttagtt taacgaatgc attgccaact
    20761 ttcaagcata gattgtcaat taaacgaagt atttttattc cttattgaaa tactaatcgc
    20821 ttttcaaaag aaaaactgag acaaaagcag ctgctttcat ctcagtcaca gatacaaaat
    20881 caattatttt tcagaatcat cggttgcttt ttcttctact tcttctttca acgcatcacc
    20941 taaaatgtct cccatagtaa atccagtatt ttcttctggt aattcataag actcaacatt
    21001 ttttggttct tcttctgaag ctggtttttc ttctaaagct ttgatactca atgcaatacg
    21061 atgttcttct ggatggatct ctaatacttt cactttcact tcgtcacctt catgaagcac
    21121 ttcatgtggt gtcgcaatat gtttatgtga aatttgagag atatggacta gcccttctac
    21181 tccagggaac acttcaacaa aggcaccgaa gcttgtcaaa cgtttaactg ttccgtctaa
    21241 tactgtgcca actggagctt ttgtatcgat ttctgaccaa ggtccaggct gtgtgtcttt
    21301 gattgacaag gaaacacgtt cttgttcagg attgatagac aacactttga catttacatc
    21361 atcattgaca gccaatacat cacttggttt tgctacatga ctatgtgaaa tctctgagac
    21421 atggactaga ccgtcaattc cgcctaggtc gacaaatgcg ccaaaatcag taagacgtgc
    21481 aacacgtcca ctgagaacat ctccctcatg gatagttgaa agaatttctt ttttcttttc
    21541 ttctctttcg ttttccacta ccgctttatg cgataagatc aaacgatttt ctgatggttc
    21601 gatttcgatg attttaaagc tcaatgtttt gcctttatag tctgaaaagt ctgcaacaaa
    21661 atgatcttcc accattgatg cagggacaaa accacgtaca ccgacgtcaa cgactaaacc
    21721 gcctttgaca acatttgtaa caggtgcttc gatgaccttg ccctcttgga aatctctttc
    21781 gatatcttcc caaacttttt tagcatctaa acggcgtttt gaaagtagat agctgccgtt
    21841 ttctttatct ttgccgatcg tactgataac gactaaatct aatacatcac cgacttttac
    21901 tacttcgctg atatcttcta ccggcaaagt tgataattct tttgccggaa cgactccttc
    21961 aactccagcc ccttcgattc cgacaatcac ttgtttgtct tcaatgacta aaacttcgcc
    22021 cttaacaaca tcgccaactt ttacttcttg tacgcttttc attgcgtctt ccattgactg
    22081 gttgttctct tgattttgat caaattctgt cataacactt gtcctcctaa ccaacattct
    22141 gccgtaagta cggtactgtt tttagtttaa ttgattattc aaaaaaaata aagcgatttc
    22201 gttcgttttc cttgaaaaaa caattgtttt tcattttttt tcttaattta aaaaagaaga
    22261 taaccttttt tcaaaacaac agcttttatt gctgcaacga cttctggaat cgatttgcca
    22321 gtcgtatcga tacgaacagc atcctctgct tgcttcagcg gagagacgtc ccttgttgaa
    22381 tcataatggt ctctttcctg tatctctttc gtcagtgttt caagatcagt cggaattcct
    22441 ttttcttggt tctctttgaa acgtctttct gcacgctcac ttacgcttgc tactaaaaag
    22501 attttcactt ctgcattcgg taatacagct gtcccgatgt ctcttccgtc catgacaacg
    22561 ccaccgcgtt cccctatttt tcgttggaca gcaactaatt cttctctgac ttttccatgt
    22621 gcagagactt cagaaacagc ttttgtgaca tctggttgac ggatttctaa agtcacatct
    22681 tttgatgcta tgaatacatg ctgtccatct tcttgttgcg aaaaagagat aggatacttt
    22741 ttgatcagcg aaactagccc ttcttcatct gaaaaagata cattgttcgt aatagctaaa
    22801 taagttactg cgcgatacat ggcaccggta tctgtataaa tataattcaa ttcttttgct
    22861 aatatcttgg caacggtact tttcccagat gaagcaggcc cgtcgattgc aatactgatt
    22921 tgtgtcatga tataactcct ttttctcaga aaaaaagccg caaagcgcgg ctattattat
    22981 ttaattctta attcttgacc agggtaaagc ataaagttgt ttggatctaa gccattcaat
    23041 tcaaacaatt gatcagtggt gatccctgca cgctcagcca cttgttttgg accttcgcct
    23101 gcttgaacag tcgttgttga accggctgca gctgatgaag aaggtgttgt ttctgctgca
    23161 ttgctgcttg aatcccctgc ttgataagtt gcctcacttg aggaagtcgg tgttgtctct
    23221 gctgcaatac tgctttctga cgttacacct gtatcttgag acacagtaga ttcttccgcg
    23281 attgtactag attctactgt tgaagtctca gttgactcct cttttgttgt acttgtagaa
    23341 ctttctacta ctgatgtcga agtttcagaa gaagttgctg ctgttgcatt gttgcttgat
    23401 ccatttctga tccagaaata agcacctgct ggaatagcga tacataagac cagtaaaatc
    23461 aaaaatagcg aaagaaataa tgtatttcct ttcttttgtt gtctctgaga acttcttgat
    23521 gaaacttcat catctgtatc gtagattggt tgttcccatg gttcttgtgt ttcgttattt
    23581 tctgtagatt gatgtctatc ttttctgctc actgttttac ctcctaaact ctatcgtgtg
    23641 tattatacca taggaaaaag tgtcttgtcc taaaaaatca atgttccatg ttttttttaa
    23701 ataaatttag taaaatcgtt ccccaatgaa gttgttcagt cttagaaaaa agcttcctct
    23761 ttggcagaag ttctgctttt gctccatcaa tatcacaaca attctgcggc ttttcggaga
    23821 gtttttcgcc aaaataagct aagatgaatt ttctgcggca gttttcttca ttaatatagc
    23881 gaagcatctg ctgcaggcgg aattcttttt ctttttcctg acgtttgagc cgttccaccc
    23941 aattttctgg tttttcagac tgctggatca gctccatcca ttttttttgc agctcatcaa
    24001 agggagcttg ctctgtggca tattccaaat aaagctcaaa actttgtcgc tgttctctag
    24061 aaagttgatt aaagaaataa tggatacgtt catccccact ttgataaaaa agaattgctg
    24121 cactttcttc ctgatctctg cctgctcgac cgatttcttg tacatagttt tctagactgt
    24181 ctggcaaatc ataatggacc acataacgga tatcagattt atcgatcccc ataccaaatg
    24241 cattcgtagc aatgagaaac tgcagctgat tttttacaaa ctgctgttga agttgtcttc
    24301 tttgagctgc atcaagacca ccgtgatagt atccaactgt aaaacgttcc ctgaataaat
    24361 gatacaatcg ttcgacttct ttttttgttg cacaataaat gattgccgct ccttgtgctc
    24421 tttccatgaa gtgttcaagt tcttgttctt tttgctgcgt tttccgaaca aaaagcgaga
    24481 tattttgccg attgactgat tgtctgatct cttttggtgg tcgatcaaat agtacatgac
    24541 gtatatctct tgctactaaa tctgttgctg tcgccgttag tgccagtgtg accgggtttc
    24601 ctaaaagctt tcggattttt cctaactgtt gatattctgg acggaaatca actccccatt
    24661 gagacacgca atgagcctca tctactacat ataacgcaat attttgtttt ttcagatgct
    24721 ctaaaagaga aggattagtc aacatttcgg gactcaaaaa caaaaattta tattgcgata
    24781 gatgacggag gacgtatctt ctttcagatt gcgaaagtaa actattgaaa gcagctactc
    24841 gtttctcccc tcgtttttgt aattgcgtca cttgatcttc cattagagaa agcagcggtg
    24901 acacgatgat gaccagacct tccattaaat accccgtcaa ttgatagcaa aggctttttc
    24961 ccgttcctgt aggcaaaata gcgagagtat cttctccatc aagcaacgcc tgaataattt
    25021 cttcttggcc gggacgaaat gaatcaaatc caaaatattt ttttaattca tttattaaca
    25081 ttctgtatcg cttcctttct caatagatag atttgataca aacgaaattc tccataatct
    25141 aattgtccat ctacattcca ttcgctaaag cgccactctc gcacggaatc cttatttggt
    25201 tccaaacgtt cgattgtttc tgcagagaga atccactcgt atggaaaatc atcaaaaaac
    25261 aaggcccact caataagatg atctgttact gttcctcttt ttaaatgacg gattgccatg
    25321 atctggtcaa ttgtctgacc attcataaac atctgcttgg tgatcatcat gctttgattg
    25381 aaattttgct gtaataatga atcgatcaaa gcgtgccaaa gcgaatctgg ccgtttttcg
    25441 atttgtgcta acaaaaggtg gatacatctg ctttgttgca gataaaccgc tgactcatca
    25501 ttgttttttg ccagctgata aggaagcagc cctgttcgct catttccaga taattggtta
    25561 gctaaaaaat ctgctgcttc ttctggtatc tccgaaaaca gctgcaccaa actctcatac
    25621 gcagtatcaa tcaaaatttc acgtggcaat tggctttcac taagccattt ttttagttgg
    25681 aaagtataaa aaggacttgt ctctgctgga agatagtctt gaatacccgt tgccagatta
    25741 gaaattactt gaacagcaaa tttaatgagc cgccaactag tttcccctgt tctgccatag
    25801 cggtcaaaac gcaaaccagt atgttctatt tgatgttcgg acaacatatc agcccctgca
    25861 ggcgttagct ttgcttcatt ttcatttatt tcgatccaac cttgctgcgc aattttttgc
    25921 aaaatttgat aaaacttatc ctgcttcaat tctggaaaac tgttatggat aaacaaaagt
    25981 tcgtgggtaa atccataaat aagtactgaa gtcgttcgtt ttcccacaag gagttggtaa
    26041 agagaactaa tccgcagctt gtgtcctgat gaaaatagcg ataaaataaa aacaatcata
    26101 ttattctcct taagttagga tggttagaaa tgtcactact atgtaaactt gtacctgaac
    26161 gctgtattgc atgcggtctt tgtcaaatcg aagcgccaga tattttcgat tatgatgaag
    26221 aaggaatcgt tttatttgcc caagaaccag aggctcatca acagttcgtg atgccggaag
    26281 aagaaacata tgtaaaaaaa gcgtatcaaa aatgtccagt tcgagcgatt ttattagaaa
    26341 ataaaatata aatataaacg aaactttaaa aacgtacact aagttctcta aacttgaaaa
    26401 taagccggga tctgtttctc ttgttccgtg aaatcccgac ttattttgaa agattcattt
    26461 gctatctata gttaattgac tagttttttc acggacccta taggacccat ttatattatt
    26521 tcaacttttt cagccagttt tccaatgtat tgacgatcac agtttgctgg tctgcgttag
    26581 aaatttctgc ctctccatct cctttttgct caccatagct gccaaaacct ccatgatttc
    26641 cgccattgat tgatacatac tctgttcttt ccggtaaata ctgcttagct tcttgataag
    26701 cttcctgatt caatacttta tcttttgtcg ccgtgatcga taaaacagga acagctgatt
    26761 ctgtcaatgt tccttttttg tcgggataac ttgccatgaa gaatacccct tttaaatgtt
    26821 tgtctgaatg attcttattg acataacgac ttgccattac gccacctaat gaatggccac
    26881 cgatcacata atcggaattt ggaaaatctt ctttgacctt atccgctttg tttccgccca
    26941 tcactgctag atcaagtggc attttgacaa tagcgactga aaagccgttt tttgctaaat
    27001 cttgcgccca aatactataa ctttctggct ctacgagagc accaggataa aaaataagta
    27061 atggagactt tccatgattc tttttctctc cttcaaaaaa taagtaatct tctttgtttt
    27121 ttccttcttt taccaacact tgcgcttctg acgatggttc gtacgtattt tgctgtatat
    27181 agtaataacc ggctccaccg agtaatccta aaatgatgac taagccaatt agtattcgtc
    27241 taaatatttt cactttctcc caccttttct atctgagtga cccctttttc ttaatcaagt
    27301 atgattatac cttaaaaaaa taagagagaa atgcaaactt tccagcatca aataaaaaaa
    27361 gacggaatat ccatttgaat atccgccctt tttgtatgtt tatttttatt tagcacataa
    27421 acgatacaca gcttctgcat aaatttccat tgctttatac atactactta attcccatcg
    27481 ttcattcgcc tggtgcataa agtcaggagt atcagggaac atcgcaccga atgccacaca
    27541 ctggttcatc gtacgagcaa aagtagcgcc gcctgataca aacggttcag tcatgtctcc
    27601 agtaatatcg cgatatgtcc ccaatagatt tttcactaac tcactgtcta gtggcacgta
    27661 taatgaatca aggaagtcaa attcttcata agtcaaccca tatttttctg ctgttttagt
    27721 taatttagct actaaatcat ctttcttgaa tgtaacaggg attctcatat ctacaccgat
    27781 ttttgtttgt tctggtgtga tttccaaact tgcaaagttc atcgttagct cgccggattg
    27841 ttcatcttct gttttgccca cgacagcttc accagttgca ttttcttgaa ctaattttcc
    27901 taagaaattg attgggccaa aatcaaatac ttctgaaaga gcaattgcca gacgagaaat
    27961 agcattcact ccttgtgctg catctttcgc gtggatactt ttaccaagaa cgacgatgcc
    28021 ttcaccttgt tcttcgaaat cgaattcgtg ttttttcaat gcttctttta cttcagaaag
    28081 cttttctcca gagtaaggag ctgcatcagg aacaacattc aacgctcctc cagcttttac
    28141 agaaaactcg ttcgttcctg gtcctgtcaa gtaagcttgc aataagcctt tttctgcata
    28201 aattaaagga aattcagcat ctggcgcaaa tccttgcgta attccttctt ctttttcgtt
    28261 atatttttct aaacaacgcc ataaattttc ttcatctgta ccaaagataa aacgaatacg
    28321 tgtcttgaat tccacgccag catcaatcaa agctttgacg gcatacattg ccgcaataga
    28381 aggacctttg tcatcttggg atccgcgacc aactaaccag ccgtctttta tagttggatc
    28441 gaatggtttt gtttcccaat tattttcgtc ccctgctgga acaacgtcca tatgacacaa
    28501 gataccgaat aattcatctc cagagccaat ctctgaataa ccgtaatagc cttctggatc
    28561 tttaaatgtt ctaaatccta gcttttcact gatttccaat actttatcca aagcttcaat
    28621 cactttttta ccgaagggat gtccttgtcc tgaatctgct tcatctagta cggaaggaat
    28681 tcggatcaat tcgctcaatg attctaatgc ttgttcatga tgttgttctg tcacaaattt
    28741 tttcatgata ttatcctcct aatatgttaa aaagggtcat gtcaaattcc tccgacacaa
    28801 cctcttcttc tcaaataatg acgttttaag cctgctccat cagcaagcag aatgttctta
    28861 acaaatgatg aaagaaaaaa cactcactga atggagctat tgacttatcc acgtgcttat
    28921 cgatagccgt ttatcggttt cttcattctt atgatagacc ttgtacctgt atttgtctag
    28981 gtttagaaca ttgctgctac acctaggatc acacagatgg ctacaaataa tatcgcaatt
    29041 aatttaccgg taaatttcca ccacactgaa acatcgaaac gagcaattgc tacagcaccc
    29101 attacgatag cagatgttgg tgtaattaag tttactaaac cagatgcaga ttgataagca
    29161 gtgattacta aatccttacc gacgccggca aattcaccca taggtcccat gattcccatt
    29221 gttgcagcag ctaagccgga agttgaagga atcaagaatg acatagggat atagaaaata
    29281 aatgtaataa tgatgaatac acttgaagat aatgaactta atccttgttc gccccaatgt
    29341 aagatggtgt ctgtgattaa tccattgttc ataaccactt ggataccacg agcaacagct
    29401 actacgattg ctacaccgat caaatcagaa gcaccagcta aaaattcaga gacaaattca
    29461 gactctttca tgccataagt gattgctact acgatggaca ttactaagaa taacatagta
    29521 atttctggga agtaccagtc acctaatgga agcatgcttt tacctagaac cgtacctagt
    29581 accggaaggt ttgttaacca gtttgtcaat gattcaaaaa tcgtgaaatt cgcattcaag
    29641 gttgtccaag gaatcaaact gatgatcatg ataccaaaag taataaagaa caatacatta
    29701 acatgttttt gttttttcgt gattacttct tgacgaccga ttgcttcaat atcgaaatgc
    29761 ttcatatctt ctttacgttg gttgtatact aatgattttg ttggatcttt ttcgatcttt
    29821 gatgcataac gatacacata tacaatagaa atagctaaca aaataacgaa catcaagaat
    29881 cgaagaccaa taccttcacc catcgaaatc ccaacagctt gtgaagctac gcctgttgca
    29941 aaaggattta ccgttgaagc tagacagcct acttgagacc ctactaatac gattgctaca
    30001 gctactactg tatcaaagcc tacagccatc atgactggga tcagtaaagc atagaatgga
    30061 atcgtttctt ctgccattcc gtaagtagag ccgcctaatg caaataacag catcaatact
    30121 ggaataagca ttttttcttt tcctttgaat ttcttcacaa ctgaagcaat cccggcatct
    30181 agtgcccctg ttttgttgat aatacctaaa aatccaccaa caaccaaaat gaagaaagag
    30241 attgggattg cagcaggtgt ttctttagtt agctcccccg ctggtacacc tagcatccca
    30301 ttgattggcg ccatgaaaat atcccacagc ccttgaggat tttgttgtac tcgttcatat
    30361 gatccagcga cgatgttgcc tgcatcgtct acactatagt gcccagctgg tataaaccaa
    30421 gtaagtacag cgattactgc aataataata aataagaccg tatacgctga aggcatttga
    30481 aattttttct tttttgtttg ttccatcttc ttcttccttt cttttccgtt agagacaaca
    30541 attttggctc taatgttatt ttatcaaaaa gagtatcaga acaaaaataa taacacgcat
    30601 tttgtaaaac ttttgttcaa aaagttcgtt tttccctatt ccctcctctt ttttcaggtt
    30661 tttaaagctt tcttcttttt cattgtagcg tttccttttt tttataaaca aacattttcg
    30721 tcttttaaaa ggagaagaat ttcacatttg caaagaattg attgattttt atgcataaat
    30781 caaaaaaata ctcaaaacaa acaaataaaa cgtttacatt attgcatgtc ttgtattttt
    30841 ttccaaaaaa aaagaccaag aaatttcttg atctttttga tatttatttg actgctgtat
    30901 gacgttgttc gaactgatgt tgttttcgac ttaaccatgg aagaagttgc gcatgcagca
    30961 ctaaaaagac agcactcacg atgaatcctt tgatcaagtt gaaaggaacg atcccaatca
    31021 gtacatagtt tgctaaagac atgcccagca tctgtgttgc gttcagccct aaaaacatta
    31081 agtaaagagg cgtaataacg aaataatttg cgatactcat aaaaatagtt agagcgatgg
    31141 ttcctgtaat gacacctaaa aatttgtttc ttcctttatg ctgtttcttt acactaaaga
    31201 aataataaat cgggaatgta aagatcgttg ttgataagaa actagcagtg tctcctacca
    31261 aattatccgg agaaaaacct gtagtcagca gatgtaatac tgaacgcaag aacgctgttg
    31321 caactccggc cgctggaccg aataagaaca tgctgatcaa taccgagata tcactaaaat
    31381 cgatcttcaa aaaactaaaa gccggcatga tcggaaaagc aaaatactgt aaaactaatg
    31441 cgatcgcagc aaacatcgct actgcaacca ttttctgtac acgggtgttc ttcatttgaa
    31501 atcctcctgt taggactcct cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa
    31561 ctctattttc cagggaaaat agagttagag ttatttgatc aagcgtcgtc aaataagctc
    31621 tatcttcttt atccagacta tactgtcggt atcggaattt caccgattca gctatttttt
    31681 tgcaaaatag gtcgtggact ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg
    31741 aaccttatat aatttttgtt tacatattca ttatacatat ctattcaaaa aacgcaacat
    31801 ctaacactta taaaaagtaa gtgttcattt tgagtggttc agcaaactgc tgacttcttt
    31861 tttgttcagt tcacggtaat ccccaggacg taggccttgt aaagtcagat ctccgtattt
    31921 ttcacgtttt agtttttgca caggaagacc aactgcttga agcatatttt tcacttgatg
    31981 attacgtcct tcgtggatcg tcaattccac gatactgtgg tttgtcttag gatccactga
    32041 taaaatttgg aatttagctg gagatgtttt tcgtccttcg atctttattc cttttgtcaa
    32101 aggtgcaagc atcgattttg tagcgatccc ttttaccttt gcaacgtaaa ctttatccac
    32161 ttcatgtttt ggatgtgtca ggcgctgagc aaaatctcca tcatttgtca gtagcaagat
    32221 ccccgaagta tcgtaatcca gtcgacctac aggatagata cgttctttta ccatcggcaa
    32281 aagatccgta acaacttttc ttcctttgtc atctgcaaca gcagaaataa ctcctttagg
    32341 tttgtacaat aaatagtaac cgtattcttc ttgataaatt gggacttcat ccacttccac
    32401 agtgtcatgt ttcccaactt ttatccctaa ttcccttaca acttgtccgt ttacttttac
    32461 acgtcctgca aggatgaatt cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat
    32521 tactttctgc aatctctcca ttacaattat tctcctttat tcattccatc aaagaataga
    32581 tctggtgaca cgtcttcttc aagtgaaatt tccatttctt gtgtatcagg cagttcttct
    32641 aatgatttca aaccaaaata atctaagaag tagccagttg ttccatataa tatcggccga
    32701 cctggtcctt ctactctacc tttttcttca atcagtttat gggcagctag acgctgtacc
    32761 gctcctgaaa cctgaactcc tcgaatctgc tcgatttcag ctcttgtgat tggctgttta
    32821 taagcgatga ttgctaaggt ttcgacggcg gcttgagata atgtgtttga tgtcccttgc
    32881 gcatatcgct taagtaaggg agccagctct tttttagtcg ttaatacata atggctttcc
    32941 gtttcaaaaa tatgcaaagc tgagtgcgag ttttgagtgt aagattcttt taattctttc
    33001 actaaacttg tgacttctgc ttcgctgaga tttaacagtc cgcttaattc ttcattactg
    33061 atcccttcct cgccaacaat aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc
    33121 ggtatctcct actgctggat aaagcataat tgattcatag ttttcttttt gttgcgcaat
    33181 gatctgccct tttttcatca attctagcaa agccataaaa gtagtgacaa cttcactttt
    33241 agaaggtgac tcaaaaaaag aatcgaaagt acagccatgc gcatttcttt ttatacgctc
    33301 ttcgataaat accattcgtt cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac
    33361 tttttgagaa aatttcttac gttctaagat tttctgcatg gcacggatca aatcgatttt
    33421 tttgacttgt cctcgcttca acggcttttc agcatccgct aacccatcga tgttcatagg
    33481 ttcttttgtg taataaagac ttcgttcctc tgctttttct gtcagtcttt cagctgcata
    33541 tttatattta cggtattcta aaagctgagt gaccaattct tctcgaggat cttcttccca
    33601 taattcttca tcgtctgtga cgaattcttg gacaggaagc aatgtcttgc ttttgattgc
    33661 catcaatgta gcagccatca ctaagtattc tcctgcaaca gctagctcga gtgttttcat
    33721 tgtatggata taattcatat attgctccgt gatcgtagcg atcggtatat catatatatc
    33781 gatttccatt tgctgaatca gatgaagcag cagatcgaga ggaccctcga atacatctaa
    33841 tttaatattg atctccgtca actcattcac tctttcttct ttttctttgc tagccatttt
    33901 ccaatgtaag gctctgtgat ttcagttgca cggatggaga catccgggta cttttcctgt
    33961 aattcatcta acatttgaaa gcccatattc tcaccgcgat aagacggatt caaagaaatg
    34021 tcatggatga tcagctgatc gttcgttgcc cagtaaatac caataatccc ttgaatgttg
    34081 gttgaacctt ctggcgtaaa gaaaaaaagt tcataatcgt cttcagattc gtacgtatca
    34141 atctccttca acaaagaagt tttacctgtc agtttcttat ggaaactcaa tagtcccata
    34201 gcaattttac ggttttcttt ccggtaatgt gttaacatct tctcaccttc ctatgactaa
    34261 gcccttggaa aatgctgctt ataaacatcc gtcattcttt tcttcgtgat atgtgtataa
    34321 atctgagtcg tagagatatc cgcgtgacct aacaattcct gtactgtccg caaatcagct
    34381 ccattttcta gcagatgtgt ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg
    34441 taaatcccag cctctcgtac taactgcttc agatttttcc agattccttg ccgagaaaga
    34501 cctttcccat gattattgac aaatacatgc atttcagatg cgtcctttac aagtaatgga
    34561 cgtgcttcgt ctaagtagcg ttccagccat tggatcgcat aatcacctaa aggaatgatc
    34621 cgctctttat ctccttttcc aactgtctgt accagtccaa gagaaagatg aagatctccc
    34681 aatttcaatc caatcaattc actgacgcgc atcccagtag catacatgac ttccaagatt
    34741 gcacgatctc ttatccctaa cgtttttgtc gtatcaggtg cttcaatcag cttttctact
    34801 tctgtcagag tcaatgtact tggtaatttc tgtgctttct tcggcgtatc gatatgttgc
    34861 atcggatcat gatcagtgat acgctcttgc cgtaaaaact gatggaatct tctgagactt
    34921 gagatcatcc gagtaacagt tgctgtcgct tttttttctt catgcaactt ctccagaaaa
    34981 cggatgacta aaaagcgatc gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa
    35041 tgcaagtact gttccaaatc acgttcatag ctttgtcgtg tattggcgga caatccccgt
    35101 tcaatattca agtagtggag ataatcgttg atttgctctt tcataataat ccccctcatg
    35161 cctctctatc atatcagata ccgcttcatt ctgatagtct ttttccataa tctgagcaaa
    35221 gaaaaagccg tctagacact cagacggact ggtttctttt ttctttttca tagcattcct
    35281 gacagattcc atggaacgtt agccgatgat cttttactaa aaaatgataa cgttcttcta
    35341 ctacttgctc cacttcaagt aaaagatcct cttctacttc ctcgatcgtg ccgcagttca
    35401 agcatagtaa atgatgatgg aaatgtttgg cgccttcttt tctcaaatca taacgggcta
    35461 atccatcatt aaaacttact ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc
    35521 gataaacagt agcaagtcca atttccggac tctttttttt cactaaaaag aagatttctt
    35581 ctgcagataa atgctctttc tcattttcca aaagcacaag cagagtagct tctctttgcg
    35641 gtgtcagctt aaaccccgct tcatgtagct gctgttttgt ttttttcatc gccgaaatag
    35701 cattcatgac catttactca accccaacaa tttataataa ttacaaataa aaaaagatat
    35761 aacactatgt taattaaaat tattataagc tagttaagaa aaacatgcaa gggattctca
    35821 attagatttt ttgactagtt ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa
    35881 aagattacct aatgcgcgct taaatgctcc tttgctgata ccaaaagctt tcatgatttc
    35941 ttctggatcc gatttatctg taaagttgat ttgctgatct gcagaacgtt ctaaaatggt
    36001 taaaatcatt tgtgcatcat ctgatatagc ttcatatgct cttggcttca atgaaagatt
    36061 cagtacaccg tctggtcgga caccaatcac tcgacctttt atctgctcgc ctagacgagg
    36121 ttcattatat cgttcagaag gatggataaa cccaagatag aaatcttccg ttaaaacgta
    36181 cgtacctgtt aatttcaagc ggtatgctgt tcctatcaca tctttgtttt tcatttcttc
    36241 tgttccatat ttgcttaaag atttgaatat tttttcatct gctaaactgc cccaaatacg
    36301 gtcttttgca tcaactctaa gggaaatcat caagcgatcc ccttttttag gccatagctc
    36361 tgtcattgtc ggcaattcat caagagatac gacaaagtct ttatctggaa gaccgatatc
    36421 gacaaacgca cctaaatctt ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat
    36481 acggctttta ggaatttgtg tagtgatccg attttcatgt ttttgatttt ggtaaccgaa
    36541 tccttcaact gcttcaccga ttttgtgttc accttctgat ttgcttaagc caaaggtttg
    36601 tccgtttttt tggacaaagt agtttttttc attttcatca ataataatac ctgtaaatat
    36661 agttgctaat aaatcgttca tttttttctc cctctatagt gaaaggtttt cttaatacat
    36721 ttctttgcta tcataacata atgttatcta cttgaatagc tgagccttgg tttggaaagg
    36781 tgaaaaaagg agtatgacaa cagttgtgtc atactcccct ttttcgaata aacggtgtta
    36841 aacggcaggc tcacagcctc tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa
    36901 ccatccagtt ttacagttga tggttacgat gtaaaatggc aaagcacttt ctccaatgcc
    36961 tttcatcaat gcagattttg ttgaagtggc atatagatta taatagcctt ccccattttc
    37021 gattctggct ttttctaatc tgtaacctcc ttcaacgata tatccacgtt ggatacatgt
    37081 agcaatcact ttgctttgga cacctggtgc ccacgtccat gctggattag atgcatcggc
    37141 aatatcactt gctctggcag atttgattgg ctgattcgct gagtttgaag aggaaggggt
    37201 cgaggatgcc gcttcttgct gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc
    37261 tgcagctgct gcttgagcgg cttgttttgc ttgttcttca gctgcttttt tcgcttcttc
    37321 tgcttttttc ttttcctctt cttctttttt ctctttatca gtgagcatct tatctacttg
    37381 tttcaattgt tcattcaact gagcagatag atctgtattt ttcacttttt ctactgcttc
    37441 ttttgccgtc ttgtattggt cgcgtgttgc tgaatccact acttttccgt ctttaaagac
    37501 tatagctgtt ttttctttag ctgtttgcaa ttcagcatat tgatttttag cttctgtttg
    37561 tgcttgttga atcaattttc caaaatctgt gttatccgtt tcgattgctt ggatctcttt
    37621 atctgctttc aacttgatgt catctgctag atgatcatcg acgatggcag gtgctatgaa
    37681 ccactcattc actttttgga tatctcggat cttagattgg atatcttggt aagttgtttc
    37741 taactctgaa tatccttttt cattcttaag actggataaa tcatttttta gttttgataa
    37801 atcttgatga atcatatctg cacgaataaa ttgatggtga tcatctgtat aaaaggcagc
    37861 aagtttctca tgaagttctt ccatttgttg ctgtcttttt gcttctgctg ctacttcggc
    37921 ttctactttt tgatgatggt cataataagc ccagcttcct ccaccgagta gaagtactgc
    37981 tgctaaaacg agataaggta ctttttttga ttttttcttt tgcgctttat ctggatctgt
    38041 tttttctaag tttgattctt gatgtgtttt ttcagtatct ggcacgattg ttattgattt
    38101 cgcttctgct tcaaacagct taggctggtt ctcttctttt tctatttttg cttcaagatc
    38161 agtaccctct ttgcttacag attgattttc agcatcggat tcactattta tttcatcggg
    38221 ctctgttgac tttttgtctg tgttttcgag tatttctttt ttctctgctt ccggcagttt
    38281 attcgttttt tccttttcta attgtttttc agtaaatact tcagtttttt ctgtgatatc
    38341 cgtttttacg tcttttattt ttactgtttc tttacctgtg gcgttttctc tatcagagtc
    38401 tttttcagtt tcttgttcct tttctggctc tactttgttt tcctgtgcaa ttttttcact
    38461 gatcattttg agcagctctt tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat
    38521 ataagcggat agtatcgaat tttcttcctc attatcgacg atttctttca gttcagctac
    38581 tcttcttttt tgttttgatt caagcgtttc ttcttgattc tttcctgatt cttccgaagt
    38641 ctcatgaaca gttgctttct tctgttcatt tttctctata ttagttactt gatcgtgagc
    38701 aggtggttct tcgtcttctg gttctgcttt ttcgaataat tccattacag attctagagg
    38761 taaatctttc aattccgacc attcgattgg atcgtttaat tctgtatcag gaaagactgc
    38821 atcttcagtt gatttatcct gcatagctgc atctgtattt gtttcttcaa ttgattcttt
    38881 tatttgctga tcttctgcag ttatttgatg accgcagtct ggacagatag tattctgctc
    38941 catttttttc gatccgcaat ttggacattt tttcatcact tagcgatcgg ctcctttact
    39001 aggtttacgc taccatttaa tctatcatat tctatacgtc acgccaatag cttattttta
    39061 ggatttactt aaaaataatg aatgaatttc cttcctatag aaaaaaaagc ttgagactgc
    39121 gtagtctcaa gctttttttc ttttaaaata aaccaaacag ttttactgat ttctcttctg
    39181 gtcaatagtc cacgcttatt ctaagaaatc ttttaattgt tttgaacgag aaggatggcg
    39241 caattttctt aatgctttag cctcgatctg acgaatacgt tcccgagtaa caccaaatac
    39301 tttccctact tcttccaatg ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag
    39361 gacattctct tcccgatcag tcaatgtatc tagtacatct tccaattgtt cttttagtaa
    39421 ctcataagct gcgtgttctg ctggacttgt tgcttcctgg tcttctatga aatcacctaa
    39481 gtgagagtca tcttcttcac cgattggcgt ttcaagggaa acaggttctt gcgcgatttt
    39541 caagatttct cggacttttt ctgttggaag atccatttcc gcaccgattt cttctggcgt
    39601 aggttctctt ccaaggtctt gcagcaattg acgttggata cgtatcaatt tattaattgt
    39661 ttcaaccata tgaactggaa tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat
    39721 ggcttgacga atccaccatg tagcataggt tgaaaactta aatcctttgc ggtagtcaaa
    39781 tttttcaact gccttcatca gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat
    39841 gcctcgtcct acataacgtt tagcgatact tactactaaa cgaaggtttg cttctgccag
    39901 acgctgtttc gcttcttggt cgccttcttc gattttcaat gctaacgcta cttcttcttc
    39961 cgcagttaaa agggagacac ggccaatttc tttcagatac atacgaaccg ggtcattgat
    40021 ttttacaccg gttggcgcag aaagatcttc tgtttttgct tgttcagctt ttttctcagc
    40081 actcttcaag ctgtgaattg atggatcacc attttcatcc accacactga tacctgcgtc
    40141 ttcaatcttt tggatcaatt tgtccatttc atctgcattc aaagtgaatg gtgtagcaag
    40201 ctgattggat aaatcatcat atacaacttg tcgtttgggt ttgttttctt tgatgaaagc
    40261 tgctactgct gcttcatacg tttttttatc tgttccattt gccataaaag aaggcccccc
    40321 ttgagtagct atttggcttg ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat
    40381 tcaagctcaa gctgtttgtt tccagttcgt ctggcttctt gctgcatttg tttttttaat
    40441 gttatttcat cagctaagcc agatttgctg atcacatcta gcaagtcttg gatttctcgt
    40501 ggcgaacttt cttctgccac cggtaaatat tcgatttcca ccaccaaccg ccttaaattt
    40561 tcatcttgca aaaagtctaa aaactctgca aggttgaatt cataatgttg tgagacatat
    40621 gcatcgaata aaaaataaac ttctgcatat gcatcatgaa taaactgaaa ttctcgttct
    40681 tttaaaatat tacggacaga aacttcttta aatgcacgat ataatagaat ctgctctgcc
    40741 ttttctactt gcgtcaaagg tctctttttc cgtgcagttt cttgtctcgt aacctgagtg
    40801 tgttgacttt cctgaggttc ttgacgattt tgctgatttg tccttcgaag atcgcgcagt
    40861 tgtttttgta tcgtctctct gccgatctga aactctgttg acagttgaga tagatacata
    40921 tcttgttcga ttggtgaaac aactcggcta agttcaacta gtaattcttc gaggtattcg
    40981 atcttgtctt tttcattatc taaatttttc ccttgtttta aataacgtga tttaaaagta
    41041 aagactgttt cgcgcccatg agccactaat tcttggaaag atgcttctcc atacttacgg
    41101 agatattcat caggatcaag cttttctgga acgactacga cagaaagtgc taatcgactg
    41161 ttttgccgaa gcaattcaat ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca
    41221 tagcaaaaga ccaattcttt cgtcagcttt tccatttgtt ggatctgttg agctgttaga
    41281 cttgtcccca tagaagctaa tccagttttg ataccagcct gccatgcagc aatcacatcc
    41341 ataaaccctt cgaacaacaa aatttctccg tttttccgga tatcgcttct tgctttatca
    41401 agattaaata aaacttgtct tttattgaac agttctgttt ctggactatt caagtatttg
    41461 ggctgatctt tttcctgccc tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc
    41521 tggccttgag aattacgaag tggaaacatc actctttgat aaaaacgatc agacatacgg
    41581 ccatcatcat gcgtgacaaa caatccagag cgttctaaca attcatttga aattgattca
    41641 ttcttgaata cttgctgaag aaaagaacgc tcattaggtg caaaacctat gttgaattct
    41701 tcaattaatt ctaaagacaa cccacgactt tgcaaatatt ctaaagctgg ctgtccagct
    41761 tttgtatgca atagcatgtg atgatagatt tccgctgctt tttcatgagc agcaatcagt
    41821 ttccccgtag cagattcagg attttggaca gctatagtac gctgacgcca ttgatcttct
    41881 aacggtatct gttcgaagtc tgccactttt accactgctt ctggaaaact tagaccttct
    41941 agttcttgaa gaaaagtaaa cacgtttccg cctttaccac aaccaaagca atgaaaaatc
    42001 tgcttgtctt ctgcaacaga aaaagacggt gtacgttctt catgaaaagg acacagcccc
    42061 aagtaatttt taccagactt tttcaactgg acatattgtc caatcacctc aacgatattc
    42121 gtttgactgc gtactgtttc gataacctct tgaggaatcc gttgagccat tttttcaccc
    42181 cctgctctat tgatatcttt tgggtttaac agaagatatt tcaggagaat aaccggtaaa
    42241 aaatcgcact aactgaaatc agtgcgattt taaaggacac aattatacat gtttgattgt
    42301 atcatatttc attgcttttt caagtataac acatttctat ctcattaagc atcaatttgc
    42361 tactcttttc agtaactgat tttgaatatc agccttccag tacaatactc gaagaacaat
    42421 atttacgagc atgaatacaa agaagtatcc tacgctctct cccaacccta agtttacttc
    42481 caaaattcca tggaacagca ttgcagctac atatatccct acgatcgtcg ttactgctag
    42541 gaagatgcgc aatgggttga agggataaca agcttttaca actgccatgc aactgatccc
    42601 aatcagcaag taatacatca aagtagtagt ttcagcattt ccccatgcga atgtttttcc
    42661 aagtagatac accgcaatga tattcactac tacaagcaag gcatttggta aagcattaat
    42721 aagtgctgta ggcaaataac ggtaagtgat ttttctttta tcttgttcaa aagataaaaa
    42781 gaaggaagga tacccttcaa tcgctaaatc aatcagcgtg atttggatag ggataaacgg
    42841 aaatccaatt gctgtaacgg cacatataat cgataaaata aatgaataaa tcgttttgat
    42901 aaaaaagatg cctgagactt tcgtcacatt gttgaccact cggcgtcctt caaacaggac
    42961 ttctggcaat gtagtaaaat cagagtccaa caataccaga ttcgcaatct gacgtgttgc
    43021 tccatcacct tctgccatag caatgctgca atctgcttct cttaacgcca aaacatcatt
    43081 cacaccgtcg cctgtcattg caaccgttct gccgctttct ttcaattcat ttaccagcaa
    43141 tttcttttgc tgcggactca ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac
    43201 ttccatttct tcagtaattt gagaaagatc gacatacgca tcataattcg gtaatcctgc
    43261 acgtctagcg atattggaga cagtaaaagg attgtctcca gaaatcactt tcagatccac
    43321 accttcctca tgaagataag ctagtgtttc ttgagcgttt tttcggattg gatcatcgat
    43381 ttcaaacaga gcaaccggct caacaaatgc tggtttttct tcgttcaatt gctgatttgg
    43441 agcgatcccc aacataagta cgcgatagcc attcgcttgg gctgttttta gactgtccgg
    43501 aagccgatct tcttccacca aacgttcagg cgctcctacg tacacgactc ccagttctgg
    43561 aaattccatc gcgccccatt tgcgatcaga agaaaatgcg actttgtttg tcacatcata
    43621 ccgattagaa agctcaaaat aatctctcat agcccgcata gtgatgttgt tatcactgct
    43681 ttctgccaga tacgtcccca taatttctgg gatggctttc tcgtacagcg gatgtagtgc
    43741 ttctacccgc tggatactca ttttcccttc agtaatggtc cctgttttat ccaagcataa
    43801 tgtatcgaca tgggccagcg tttcaattga atacatgtct tgaacaagga ttcgtttttt
    43861 tgccagtttg gttacccctg tcgttaatgc aatacttatt agcaatacca aaccctttgg
    43921 aagcatgcct aacaaagctg ctgcagatgc aataacagaa attttgatag aagcatcgcg
    43981 gatgaacagt gcttccaaaa atagtatgat ccccaaagga ataatcacga aactagtgaa
    44041 tttggatact ttgcgaatcg aatctacaag ttcagaacga atagctttgt gagtctttgc
    44101 ctcgctagtc aagcggacag cataattatc tgcacctact cggataactt cagccatgac
    44161 agaaccgctt gtcaaaaagc ttccagaaag caattcagct ccagtttctt tgacaatcaa
    44221 atcagattcg cctgtcaaaa gagcttcatt tgcttctgcc cgtccagata taacctgtaa
    44281 atcggctggt atctgttctc ctgctccgat ttttaccaaa tctcctaata ccagctcttt
    44341 agtagaaatc gattgttctt taccgtcccg gatcactagt acttctgatt gggaaacaat
    44401 cgataatttc cgtacaagat ttcttgcatg tatttcttga taaattccaa taacgatatt
    44461 gacgacaata attgctaaat acgccatgtt ggaatacgca cctacaaata acagacaaat
    44521 cccaatacca aagttcagaa aattgaataa cgtcaatata ttatcgctga atatttgttt
    44581 tgtacttttt gatgcatctt cctggtagtc attttgctgg ccagaagcca ttttctgaac
    44641 tacctcttgc tgtgtaagtc cattcatttt ctttttctcc attttccgcc aactttcaaa
    44701 aataatcacg tgtttaagtc tagcgtgttc caagattaaa aacaacttgt ttttataaaa
    44761 tttatagaaa aacacacgaa tgattttaca aaaaagatgc caaccttttt gtttgaatca
    44821 cccgtgtgct gtttttatta tcaatctatc tttacttata ttgttcctcc aaacggctca
    44881 tccaccaacc tagtgctgta ccggctataa aaagaacagg acaagctagt accccagcaa
    44941 ctcccaatcc cgcgtaatcg gtaggaatga tcataatcgt tgaagcaaaa actacaccta
    45001 aaatgaaatg gaatagcttg ctatatgcct ttgagaaaat gtaatccatg atttttgaca
    45061 atccaagaac acaaagaatc ccaccaattc cgatcggcac aatcacttca agtcgtactt
    45121 ctttaaatcc gtcagccatc gctttataca atcccatata caccaaaaaa ttagaagggc
    45181 tcaatcctgg gacgatcata cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa
    45241 aattctgatc gacttgcccg tcaaataatt gagcgccgta taataaaaac aaataagcca
    45301 gaacaaaagt aacaaccatg atgatcacat cagaagtatc tcttcctttt ttccctgctt
    45361 ctttccataa cgcaggaaca gttccagcga tacatccgac aaaaaaccat aatatcgttg
    45421 tttcatacgc acctaataaa aaactgacta aaaaagaaag tagaaagatg cctgtcactc
    45481 cgcctagacc aacaggcaga aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg
    45541 ctaaaaaaga aatcagccgc tcatacatgc caaagacagc tgctaatgca ccaccgctga
    45601 ctcctggcaa gataaatcct gaaccgataa acattccctt cacaaaccgt aacatccagt
    45661 ctttggttgt gtaccccact tcttgatttt ccattttttg ccttttttcc actatttgcg
    45721 tactccttct ttcatttaat tttattcatg attgaacatt tcctgctatt ttacttaaag
    45781 actttttcat aggtctctat tattttttca ttcattttga caatatcagg ataaaacatg
    45841 ttacctccat tatgatagag atagccgcca ttgtaaagca aggactggat ccgccaatat
    45901 cggtattgtt tcccttcctc atttcctaaa agtgggctca gcacatcttt agaatacgct
    45961 tctgctaatt ctactgtgtt ttctccgcca tgatttttta cataggcaat ataatctttc
    46021 ccaaaattat atgcttggac agctgtccat aaatcgcatc cttgtttctt tgcttctact
    46081 atcatttccg ccaaatattt gacaccttga tcaatactgt cagctggatt atcaatgatc
    46141 tcagcttctc catgcagact ttccgagctc tgcatgagat ctgtcccttt tccttttgat
    46201 tcggtcaata ttatcgcctt gatcaaattc tcgtagtttt ccatatgata attttttgca
    46261 gctgttgtta tttctatttg atagctttcc actcgtttta ccgaacgata agttcttcct
    46321 cataaaaaca acaccgcacc aataaccgtc agaatgatca tgtaaaaaag aatacggata
    46381 atagataatc gttttcgctt catttcatca cctagttatt ttgacatctt tttttatctt
    46441 actatttctt tatcttcttg gctatttctt ccttgatttc tgtgtacgcc ttaagaaaaa
    46501 atgaattttc ctgtattttt tgacatctta cgaaaatgat cgttgttttt cagtaaaaac
    46561 aattgcccaa tgactgatat aatcgttata ctttacacag taggtgatta tgtgaaaaaa
    46621 gaaaagaaat atgcaaaaat gccagatggg agcgaaattt actatgaaaa gagcggtcaa
    46681 ggcttccctc tctttttact gcatggcaat gacggcagcg gccgcttttt ttcagaacag
    46741 gtccctgtac tcgaacgcta ctataccgtg tatctagtag atagccgagg acatggtcgt
    46801 tcgacaaacg aagcatctat gttgaatttt cagttaatgg ccgaggatct gaatacgatc
    46861 atgttgctgg aaaaaatcga tcaggcagat tttcttggat ttagtgatgg agcaaatcta
    46921 gcactggtat ttgccagcag cttccctaaa aaagttcatc gattgatttt aaattctgga
    46981 aacacgcttg ttaaaggcgt tcgtttttct gccagagtca tcagtaatat tcattatgca
    47041 tgggtatggt tactctcttt atttcgccca agtcttcgca aaaatctttt agttatcaaa
    47101 cttttgctgc atgacatcgg gcttacagaa aatgatttaa aaaaaatcaa ctcccccact
    47161 ttgatcatcg tgggaaaaaa agatgtaatc aagctaaaac attctttgta tatagctaaa
    47221 actataccaa aagcttcctt tgtgcttgtg aaagaacaag gacatgaatt agctagaaaa
    47281 gatcctgaac gcttcaatcg agaagttctt caatttcttt ctgaaacata actattagga
    47341 atagaaggtg atttatttgt taaaaaatac catacaatgg ctgaaaaacc acctaggact
    47401 gttcaaaaca atctttttga tttctgtcat tgtcattatt gtcggtgaat tgatgtccat
    47461 cggtaaaact ctatctatcc agcaattagg agaaacgttt gccactatac ctgtatggaa
    47521 aagcggacta atgctattga ttggtcttat ctctgtgtta cctatggttg gttatgatat
    47581 tattttgaat cggatgcttg aacaaaagca gaatcctcgt tacctgtttg agacaagctg
    47641 gctgatcaat acgatcaaca atattgccgg tttcggcggg tttgttagtg tggggcttcg
    47701 ctctgaacta tatggacaaa agaaagagag cagaaacgtg atccaagcat tatcgaaagt
    47761 tttcttgttt ttgatggctg gactttctat ttacagtctg ctttcttttt tacttgtagt
    47821 cttcacgcca gttgctcctt ttttgaagca atattggata tggttgatcg gcggcggtct
    47881 atattttcct gtcgtctttt tatttactac tttcaaaaag aaaggattcc ttggtggtac
    47941 ctcacttaaa actcaaggac agctattgct agtttcattg ttggaatgga ccggtgtcct
    48001 gtttagtttc ctttctgttg gttatttaat ggaaatccgg attgatctgt ggcaaacaat
    48061 tccattattc attgctgctt cagtgatagg aatcgtgtct atgattccag gagaaattgg
    48121 gagtttcgat gtaatgatga ttatcgggtt gtccgctatt ggtgtgccaa gagaaatagt
    48181 cgttgtgtgg atcttgctct atcgcctgtt ctactacatc attccgtttt tgattggaat
    48241 cgtctttttc ttcaaaaata tcggttccac ttttgatcag cgttattctg gtatccctaa
    48301 acaattagca acagaaattg cgcataaaat cgtggttgtc ttattatact tttctggaat
    48361 catgctagta ctttctgcca ctatcccgca agcttttaca gagtttcgct ggcttcatag
    48421 tttgaaccca ttgaaatttc attttattat ccaatttcct agtatactat tgggatttct
    48481 tttaatcatt atgggcagag ggatcgcagc acgtgtgaaa cgagcatatt tgcctacaat
    48541 cttcttgatt gttctagctt tactttatgt tttattaagt gattttagtt tcacaccggt
    48601 ggttttcttg tctattctgc ttctgatcat tcttgcttct aaaaacgaat tatttcggga
    48661 acaattgatc tattcatggg agtggcgtac gatcgatgga atcattattg gtgcactgtc
    48721 cttgctttac atcgttattg gggtatacaa tctgccggat ttccctcatc ggcgtcacca
    48781 ttttatttcc tttttcttat ttccttctga aaagatttgg ttttcgggat tgttggccat
    48841 catcgctgtt agtttcatga tcgttctatt tgttcatttc cttcaaggag aaaagaaaca
    48901 gattggcgaa gcctttaatg aagaaaaggc cctgaagatc cttacaactt atggaggaaa
    48961 ctccgatagt caattgatct tcttaaaaga taagcggatg tttgcctatg aaaaagacgg
    49021 tgaaccaact gttcttctcc aattcgcttg cttcaataat aaatgtattg taatgggtga
    49081 tccttctggt aagaaagaag attttccaga agcaatcgaa gcttttatcg aagaaacaga
    49141 tcgactatgc tatttaccag ttttctacga aacaagcgaa gagattgtta tgattttgca
    49201 tgaattcggc tacgatttca ttaagatggg ggaagaagct tatgtagacc tgaatagttt
    49261 tacaacttca gggaaaaaaa tgaagggcac tcgtgctgtc ttgaatcgga tcgaacgaga
    49321 aggatttaca tttgatgtct tacaaccacc attttctact gagcaaatga gtactttcaa
    49381 aaatatctct gataattggt tgggttcacg aaaggaaaaa gggttttctt taggcttttt
    49441 ctctgaagat tatctacaaa gagcgcctat tgcagttgtc aaaaataccc aagaagaaat
    49501 tgtcgctttt gcaacgatca tgcctactta cacgaacaat aaagtaggaa cgattgatct
    49561 gatgcgatat gattctaaaa aagcaccttc tggcagtatg gatttcttgt tcttgcattt
    49621 gtttgcctat atgaaagaag aacatatcca atggttcaat ttagggatgg cccctctttc
    49681 aaatgtcgga acttccagaa agagtttttt acaggaaagg attgcatact tggtttacga
    49741 gtttggttct catttttatt ctttccatgg attaagagaa tataaaagca agtacgctac
    49801 aaactgggtc tcacgttata ctctatattc tagagatagt tggattgcat atgttatgat
    49861 tgctttgctg attattgata atgcgccagt tgagcagaca caaggtagaa taagtggttt
    49921 gaaaaaatgg atacgaagaa agtattaaaa actgcatacc gatagatgaa cactctcatg
    49981 atgaataaaa gagactgaag taaaacgaaa gttcatgctt ctacaatctc ttcttataca
    50041 attgaagaac aaagagcggt ccaaacagaa atcctgtttg gaccgctctt ttattttaaa
    50101 catttttcat taccaaaata ttcgagtcgc gtttcttatg cttcgatctc tgtaacgata
    50161 cctgaaccta ctgtccgtcc gccttcacga attgagaatg tcgttccgtt ttcaacagcg
    50221 attggatgga tcaagtcaac atcaatcgtc acgttgtcac caggcatgac catttctgta
    50281 tcttctggca atgtgattgt tcctgtaaca tcagttgtgc ggaagtagaa ctgtggacga
    50341 tagttgttga agaatggagt atgacgtcca ccttcttctt ttgtcaacac gtacacttct
    50401 gctttgaatt ttgtatgtgg agtaatggaa cctggtttag caagtacttg tccacgttcg
    50461 atatcgtcac gttggatccc ccgtaacaac acgcctacgt tatccccagc ttccccgtaa
    50521 tctaacgttt tacggaacat ttctacacct gttacaactg ctttttgtgt ttcaggtttg
    50581 atccctacga tctctacctc atcaccgaca cgaacagcac cacgatcaat acgacctgag
    50641 gcaacagttc ctcgacctgt aattgagaag acgtcttcca ctggcaatag taatggttta
    50701 tctgtatcac gttctggtgt tgggatatat tcatctactg tatccatcaa ttccataata
    50761 gcagcttcag catctggatc gccttgcaat gcttttaatg cggaaccttt gatcacagga
    50821 gtgtcatcgc ctgggaaacc atattcgctc aataactcgc gaacttccat ttctaccaaa
    50881 tcgatcaatt cttcatcatc gactaaatca actttgttca agaaaacaat caggtatttt
    50941 acaccaactt ggcgtgacaa taaaatgtgt tcacgtgttt gaggcatcgg accgtcagtt
    51001 gcagaaacaa ctaagattgc accatccatt tgtgctgccc ctgtaatcat gtttttcaca
    51061 tagtccgcgt gtcctggtgc atcaatatgc gcgtagtggc gtttttctgt ttcatattct
    51121 acgtgtgcgg tattgatcgt aatcccacgt tcacgttctt ctggagctgc atcaatacta
    51181 gcatagtctt gaggatttgc taggcctttt ttgcctaata ctgtagtgat cgcagcagtt
    51241 aaagtagttt tcccatggtc aacgtgtcca atcgtaccaa tgttaacgtg tggtttactt
    51301 ctgtcataat gttcttttgc cataatttac aaacctccta aataatatag atcactgaaa
    51361 aatttcagct ctcacctttt tattatagtc tttttttgac taaatcaaaa agaatacgtc
    51421 cgacttactt tttgtaagat tcgaactaag cttattatac gtcaatattg gtcaaagtca
    51481 actaagagca ctcgtctgtc ttatacgcga aaaaagtacc ttttataaat aaaaaaatgt
    51541 gttttttgag aaaaacttca agatagtcct taactttctt ttgaagcaat aagaacaaaa
    51601 tgaggaaaag tttaaaattc agctgttgcc aacaactttc aatagacgaa atcttctatt
    51661 tcgttaataa taaggaaaga ggtgaagtac atgaaaaaat taattggcgt acttgtcgtt
    51721 ttaggcatat ttctcggcgg aagttttgca gcttatcatt atttttacgg cggcgaagct
    51781 tattatacaa aaatcacgac gaatggcgaa atgtcagcta cccaagcaga taacggagag
    51841 aaattcacga cttataccta caagcaagcg gcttataata aagatggtga aaaaaaacag
    51901 gtgacgcttc gcgaagaaag agaacatcca ttaaagatga atgcgtatct taaactcaaa
    51961 gtcaatcctc gtaaaggtgt attaagctgg gaagaaataa aggcttctga agtgcctaaa
    52021 aatgcggcag aaaaaatcga taattagtaa atggtcttaa aattttatta gataaaaaga
    52081 ggctgggaca gaagcgttta actccaagaa ataagaagga gttgcttctg cttccgccgt
    52141 ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtctcac gctctttttt
    52201 tgttatattc gtcttttccg atccttctct tgacacaatt catttaactg atcactggta
    52261 cttgaacacc taatattcta gtcattttta taaacgattc aatacttcat ccacattttt
    52321 gatcatcacg gatatcgtac catccggatt attcacaaat tcaatcaaat ctgggttgcg
    52381 atatacatct actggtacga tcagttcgat cccgtttgat agcttgaatt tttgttttcc
    52441 aaattttttc tcagaaattt cttgtacttc ttttacagga ggtgcttctg ggacaaatcc
    52501 ttgctcttgt acttcttctt taaaagccat ttttgccgtg atgttttcct tgaagacttt
    52561 ttccgcaatc atctcatgat ccaaccgacc tttttcttct atcgtatcat agacagcttc
    52621 tttcaaatca gccatgatat caaattcttc tgtctcaaat ttttttccaa gatgcttggc
    52681 tactttttgg atcactttta cattttcttc taaagaaggt gcaggaacac tttcgatcac
    52741 tcttccagaa aaataaagct cttttttgcc agaaaactca tatttttttt caattaattc
    52801 gtaggtccaa tcagtcaaat tcacagcgaa tgcttcatct gctttttgtg ttttcgcccc
    52861 tagaattgct cgattcaaaa tcaagcggtt gtcgatccct gcttcgtctg cttcaacaaa
    52921 gtgagtgtaa gcctcacgat aattgacttt caataaagat aagtacatca ctgtatctaa
    52981 ttcatacaag acaacaaata catctgcact aggtgcttct tcactttctt ggtagatatc
    53041 gaaccaccgc tgaacgattg cttcacttgc ttccacaaaa ttttcttgag cgattttaag
    53101 tgattcaacg atcatgcttt cttctgctag ttgtccagtt tttgtttgag cagaagagat
    53161 tttttggatc ttcttttgaa gaaaatcacg aatatattct tttgtcagat ccagctcttt
    53221 ttgtgaaaag actggagaac cagtttctct gtcaataata tgcagaattg ctttttttaa
    53281 atagatatcc attttctcat tcccttctgt cttagtttac ggaaaaaaaa tcaaaaatac
    53341 cagaatcaaa atcattttct tttaaacaaa aatttcaaga gtagaaacaa aatggattct
    53401 ttttttcgaa aaatgcgcta cacttatagt tgaacgaata aaaaaggagc tgaaagtatg
    53461 aattcattaa caagcactta tcgtctatca aatggttacg aaatcccggt agtaggattc
    53521 ggcacatggc aaacacctga tggggatgtc gctgtttcct ctgttaaaga agcgctggct
    53581 gcaggttatc gccatatcga tactgcccaa ggatacaaga atgaagaaag cgtcggccaa
    53641 gcgattaagg aaagtggaat tcctagagaa gagatattct tgacaacaaa attatggaat
    53701 gcaaaccaca gctatgaact tgtgatgtct tcttttgaag aatcattgaa gaagctccaa
    53761 acagattatt tagatctgtt tttgatccac tggccaaacc ctgttgcttt tcgtgataat
    53821 tgggaacaag caaatgcaga tacatggcga gcatttgaag aactgtatga agcaggtaaa
    53881 atcaaagcaa tcggtgtgag caatttctta ccccaccatc tagatacact agcaaaaaca
    53941 gcaaaaatta tgccgatggt caatcaagtt ttccttgcac caggcgaatt acagacaact
    54001 gtagttgagt atgcaaaaaa acatgatatg atcctcgaag catatagtcc attaggcact
    54061 ggtaaaatct tcgatgtgcc tgaaatgaaa caaatcgctg aagcacatga caagacgatt
    54121 gcacaagttg ctttgcgttg gtcgctgcaa cacgaattcc ttcctcttcc taagtcagtg
    54181 acaccaagca gaatcaaaga aaatactgaa ttatttgatt ttgaattaac agaagaagaa
    54241 atgaaacaaa tcgatcagtt agatggtgta gtcggcaaag caaaagaccc agatactacc
    54301 caattttaag tcgaaaggtg attttatgga tccaatcgaa cgcttgaatt ctctctcgga
    54361 agaagttacc cagacttttc attccgattt tgtctttttg atcgatgcag agaaaatcca
    54421 acattttcct gcaagaaact ggacacatga tcaaatcatc gaagaactga aaaaacggtt
    54481 cgatcattcg ctaatggtca aaccctggca cgaacatgaa gtcatttatt cacctgaact
    54541 acctgttttt gccttgatac cgaaaaaata gaaa
//
LOCUS       DAYYFD010000005        52401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000005 DAYYFD010000000
VERSION     DAYYFD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.005"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..612)
                     /locus_tag="ACHA25_000281"
     CDS             complement(<1..612)
                     /locus_tag="ACHA25_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761617.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLK"
     gene            complement(791..1510)
                     /locus_tag="ACHA25_000282"
     CDS             complement(791..1510)
                     /locus_tag="ACHA25_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8761618.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1641..2792)
                     /locus_tag="ACHA25_000283"
     CDS             complement(1641..2792)
                     /locus_tag="ACHA25_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8761619.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3009..3749)
                     /locus_tag="ACHA25_000284"
     CDS             complement(3009..3749)
                     /locus_tag="ACHA25_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8761620.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3753..4076)
                     /gene="rsfS"
                     /locus_tag="ACHA25_000285"
     CDS             complement(3753..4076)
                     /gene="rsfS"
                     /locus_tag="ACHA25_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8761621.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4133..4726)
                     /gene="yqeK"
                     /locus_tag="ACHA25_000286"
     CDS             complement(4133..4726)
                     /gene="yqeK"
                     /locus_tag="ACHA25_000286"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8761622.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4710..5360)
                     /locus_tag="ACHA25_000287"
     CDS             complement(4710..5360)
                     /locus_tag="ACHA25_000287"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8761623.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5370..5681)
                     /gene="yhbY"
                     /locus_tag="ACHA25_000288"
     CDS             complement(5370..5681)
                     /gene="yhbY"
                     /locus_tag="ACHA25_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8761624.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5697..6806)
                     /gene="yqeH"
                     /locus_tag="ACHA25_000289"
     CDS             complement(5697..6806)
                     /gene="yqeH"
                     /locus_tag="ACHA25_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8761625.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6803..7330)
                     /locus_tag="ACHA25_000290"
     CDS             complement(6803..7330)
                     /locus_tag="ACHA25_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8761626.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7510..8295)
                     /locus_tag="ACHA25_000291"
     CDS             complement(7510..8295)
                     /locus_tag="ACHA25_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8761627.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8288..9157)
                     /gene="accD"
                     /locus_tag="ACHA25_000292"
     CDS             complement(8288..9157)
                     /gene="accD"
                     /locus_tag="ACHA25_000292"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8761628.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9158..10537)
                     /locus_tag="ACHA25_000293"
     CDS             complement(9158..10537)
                     /locus_tag="ACHA25_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8761629.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10545..10964)
                     /gene="fabZ"
                     /locus_tag="ACHA25_000294"
     CDS             complement(10545..10964)
                     /gene="fabZ"
                     /locus_tag="ACHA25_000294"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8761630.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10989..11465)
                     /gene="accB"
                     /locus_tag="ACHA25_000295"
     CDS             complement(10989..11465)
                     /gene="accB"
                     /locus_tag="ACHA25_000295"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8761631.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11468..12703)
                     /gene="fabF"
                     /locus_tag="ACHA25_000296"
     CDS             complement(11468..12703)
                     /gene="fabF"
                     /locus_tag="ACHA25_000296"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8761632.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12722..13459)
                     /gene="fabG"
                     /locus_tag="ACHA25_000297"
     CDS             complement(12722..13459)
                     /gene="fabG"
                     /locus_tag="ACHA25_000297"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8761633.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13471..14388)
                     /gene="fabD"
                     /locus_tag="ACHA25_000298"
     CDS             complement(13471..14388)
                     /gene="fabD"
                     /locus_tag="ACHA25_000298"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8761634.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14495..14719)
                     /locus_tag="ACHA25_000299"
     CDS             complement(14495..14719)
                     /locus_tag="ACHA25_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8761635.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14772..15734)
                     /locus_tag="ACHA25_000300"
     CDS             complement(14772..15734)
                     /locus_tag="ACHA25_000300"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8761636.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15744..16184)
                     /locus_tag="ACHA25_000301"
     CDS             complement(15744..16184)
                     /locus_tag="ACHA25_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8761637.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16490..17455
                     /locus_tag="ACHA25_000302"
     CDS             16490..17455
                     /locus_tag="ACHA25_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8761638.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17512..17742)
                     /locus_tag="ACHA25_000303"
     CDS             complement(17512..17742)
                     /locus_tag="ACHA25_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8761639.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17828..18715)
                     /locus_tag="ACHA25_000304"
     CDS             complement(17828..18715)
                     /locus_tag="ACHA25_000304"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8761640.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18804..20027)
                     /locus_tag="ACHA25_000305"
     CDS             complement(18804..20027)
                     /locus_tag="ACHA25_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8761641.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20044..21090)
                     /locus_tag="ACHA25_000306"
     CDS             complement(20044..21090)
                     /locus_tag="ACHA25_000306"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8761642.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(21254..22981)
                     /gene="ptsP"
                     /locus_tag="ACHA25_000307"
     CDS             complement(21254..22981)
                     /gene="ptsP"
                     /locus_tag="ACHA25_000307"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8761643.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(22981..23247)
                     /locus_tag="ACHA25_000308"
     CDS             complement(22981..23247)
                     /locus_tag="ACHA25_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8761644.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23726..25960
                     /locus_tag="ACHA25_000309"
     CDS             23726..25960
                     /locus_tag="ACHA25_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8761645.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            26108..26428
                     /locus_tag="ACHA25_000310"
     CDS             26108..26428
                     /locus_tag="ACHA25_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8761646.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(26633..26857)
                     /locus_tag="ACHA25_000311"
     CDS             complement(26633..26857)
                     /locus_tag="ACHA25_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8761647.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(27055..28635)
                     /locus_tag="ACHA25_000312"
     CDS             complement(27055..28635)
                     /locus_tag="ACHA25_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8761648.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(29036..30412)
                     /locus_tag="ACHA25_000313"
     CDS             complement(29036..30412)
                     /locus_tag="ACHA25_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8761649.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(30540..31628)
                     /locus_tag="ACHA25_000314"
     CDS             complement(30540..31628)
                     /locus_tag="ACHA25_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8761650.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            31800..32234
                     /locus_tag="ACHA25_000315"
     CDS             31800..32234
                     /locus_tag="ACHA25_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8761651.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(32312..32845)
                     /locus_tag="ACHA25_000316"
     CDS             complement(32312..32845)
                     /locus_tag="ACHA25_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8761652.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(32939..34117)
                     /gene="mutY"
                     /locus_tag="ACHA25_000317"
     CDS             complement(32939..34117)
                     /gene="mutY"
                     /locus_tag="ACHA25_000317"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8761653.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(34110..34907)
                     /gene="recX"
                     /locus_tag="ACHA25_000318"
     CDS             complement(34110..34907)
                     /gene="recX"
                     /locus_tag="ACHA25_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8761654.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            35014..36396
                     /gene="rlmD"
                     /locus_tag="ACHA25_000319"
     CDS             35014..36396
                     /gene="rlmD"
                     /locus_tag="ACHA25_000319"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8761655.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(36570..37148)
                     /locus_tag="ACHA25_000320"
     CDS             complement(36570..37148)
                     /locus_tag="ACHA25_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8761656.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            37470..37940
                     /locus_tag="ACHA25_000321"
     CDS             37470..37940
                     /locus_tag="ACHA25_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8761657.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(38048..39274)
                     /locus_tag="ACHA25_000322"
     CDS             complement(38048..39274)
                     /locus_tag="ACHA25_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8761658.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(40028..40228)
                     /locus_tag="ACHA25_000323"
     CDS             complement(40028..40228)
                     /locus_tag="ACHA25_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8761659.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(40362..40970)
                     /locus_tag="ACHA25_000324"
     CDS             complement(40362..40970)
                     /locus_tag="ACHA25_000324"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8761660.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(41055..41525)
                     /locus_tag="ACHA25_000325"
     CDS             complement(41055..41525)
                     /locus_tag="ACHA25_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8761661.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(41573..42487)
                     /locus_tag="ACHA25_000326"
     CDS             complement(41573..42487)
                     /locus_tag="ACHA25_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8761662.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            42633..43436
                     /locus_tag="ACHA25_000327"
     CDS             42633..43436
                     /locus_tag="ACHA25_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8761663.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            43614..44561
                     /locus_tag="ACHA25_000328"
     CDS             43614..44561
                     /locus_tag="ACHA25_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8761664.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(44629..44922)
                     /locus_tag="ACHA25_000329"
     CDS             complement(44629..44922)
                     /locus_tag="ACHA25_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8761665.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(44950..45615)
                     /locus_tag="ACHA25_000330"
     CDS             complement(44950..45615)
                     /locus_tag="ACHA25_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8761666.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(45755..46387)
                     /locus_tag="ACHA25_000331"
     CDS             complement(45755..46387)
                     /locus_tag="ACHA25_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8761667.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(46394..47461)
                     /gene="liaS"
                     /locus_tag="ACHA25_000332"
     CDS             complement(46394..47461)
                     /gene="liaS"
                     /locus_tag="ACHA25_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8761668.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(47458..48189)
                     /gene="liaF"
                     /locus_tag="ACHA25_000333"
     CDS             complement(47458..48189)
                     /gene="liaF"
                     /locus_tag="ACHA25_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8761669.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(48358..48837)
                     /gene="greA"
                     /locus_tag="ACHA25_000334"
     CDS             complement(48358..48837)
                     /gene="greA"
                     /locus_tag="ACHA25_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8761670.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(48973..50148)
                     /gene="mltG"
                     /locus_tag="ACHA25_000335"
     CDS             complement(48973..50148)
                     /gene="mltG"
                     /locus_tag="ACHA25_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8761671.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            50352..51521
                     /locus_tag="ACHA25_000336"
     CDS             50352..51521
                     /locus_tag="ACHA25_000336"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8761672.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(51788..>52401)
                     /locus_tag="ACHA25_000337"
     CDS             complement(51788..>52401)
                     /locus_tag="ACHA25_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761673.1"
                     /translation="FMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILN
                     YSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVI
                     YSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSES
                     GINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        15215 a        10947 c         8725 g        17514 t
ORIGIN      
        1 ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg gatattctgg
       61 gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt aaattcgatt
      121 ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc cacaatccaa
      181 aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt ttttcgtgtc
      241 tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc tccactctaa
      301 tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga
      361 acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg gtgcgcggtc
      421 caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc ctaatcgata
      481 gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag ttacgttcac
      541 agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat
      601 ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt tcacatgata
      661 cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac acaaaaaagc
      721 aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa
      781 aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca tcttctagat
      841 cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat agtgttacct
      901 cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct ttttctttta
      961 atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt tgtccttctt
     1021 cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg atctcgtcag
     1081 cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa accgctccgg
     1141 aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct gctgttcgat
     1201 tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca aatccttcat
     1261 agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt gctcggtcaa
     1321 taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt agtttttggt
     1381 ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc ccaaattttg
     1441 cataaatccg gctattatta gcatctttag cagttttctt tgctacgata tttgcccatt
     1501 tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca aaaacgtcct
     1561 gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt ccttcattct
     1621 tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt ctgttccgga
     1681 atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc ttcttgtgcc
     1741 tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa gaatcttctt
     1801 ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac aggaatctct
     1861 tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt ccatgtatat
     1921 ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc agctgctgtt
     1981 ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc cagcaaagta
     2041 gaagataaca accgataacg caaatacggc caataagatt cccaagtctg cagcgaacaa
     2101 gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt tccttcttct
     2161 aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat tttttcatga
     2221 tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata cttggcattt
     2281 tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag cgtaatatcg
     2341 ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt ttgatcagac
     2401 agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg tccgaaactg
     2461 ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt
     2521 tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc ccattcgacg
     2581 ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg atgccattta
     2641 tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc aatgacgacc
     2701 tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt gtgaaaagga
     2761 ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag ttatcgataa
     2821 aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg aactatataa
     2881 actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga gcaatgccta
     2941 tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa ctcgagcagg
     3001 caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc tgatgctgct
     3061 tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc cagcattctt
     3121 aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg aataaatcga
     3181 ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg
     3241 cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg atagctatat
     3301 tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa tcggccattt
     3361 tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct atttttcata
     3421 tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc tgataaatcg
     3481 agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg ctggctggca
     3541 atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc ttttgcaaag
     3601 gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt gtcttcttgt
     3661 aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat cacttcatcg
     3721 taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact aagatcgact
     3781 agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg cgcatggaaa
     3841 acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc ttttccttca
     3901 atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc tgtaattgcg
     3961 ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa caaggaaaca
     4021 tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc ttcaatcgca
     4081 atttttaatt gttctttact atctatgatg atttcctcct aatatgagca gcttattttt
     4141 ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt tcttgcgcaa
     4201 gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga tcagcccatg
     4261 caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata tagtctgcta
     4321 cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc acccggattg
     4381 catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa gcgccaacaa
     4441 gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg tctcttacga
     4501 ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa
     4561 tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct gtttcttcta
     4621 ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt tgcatcaggt
     4681 tttctctaga ataagcagta tattcattac tatagtccat attcatacaa cccttcattt
     4741 tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc ttctttgatc
     4801 ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca aattacagga
     4861 tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac catagaagtc
     4921 agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc gataataaag
     4981 taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata ggtgtaactt
     5041 ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc ttccaccgct
     5101 aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc aacatgaggt
     5161 ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc agcttgttca
     5221 gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat ccctacttgc
     5281 tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg aacaaatgtc
     5341 ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt tgatagcctt
     5401 tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat gatttggacg
     5461 atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat ctcatctgta
     5521 ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc aatttgaacg
     5581 atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag atgatgcgct
     5641 ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct tcttccttaa
     5701 atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc aattactact
     5761 ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca
     5821 gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg aggaggctgt
     5881 aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt cgttcgatga
     5941 atatttacat catttgacac ataagcaata aatgaagcac gattgccgct gataaaatca
     6001 aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata aactttaggt
     6061 ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta gtgcgccatt
     6121 tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc
     6181 tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc ttgtactccc
     6241 gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt tactccgacg
     6301 atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc cgtcatctca
     6361 tgagatttct tagcactagt taataataca tctactggac gaattcctgc ttcgtaagca
     6421 cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa tatatccact
     6481 ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc tgggattagt
     6541 gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt ttgccctaat
     6601 tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt ctcattataa
     6661 tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc tttttccaaa
     6721 gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac ggcaccacag
     6781 ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata tcagggtgtt
     6841 ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt gtcttccaac
     6901 tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga ttggctccac
     6961 gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct aaatccaatt
     7021 gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca acatacaaaa
     7081 gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa acaacaacaa
     7141 cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct gttccatctg
     7201 gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg atgcctagat
     7261 tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt ggtttgtaat
     7321 ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag tactcctcaa
     7381 ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc gtctttcaga
     7441 aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa atttagaaac
     7501 aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt tatgctgatc
     7561 taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt gtgcgacttt
     7621 atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa cgattcctaa
     7681 ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg cgcgactacc
     7741 gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa tcgtttcttc
     7801 cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc caccttcacc
     7861 aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat ttctggcaat
     7921 agcctctcct tcgccgcgct cttctgctcc tacaccgcaa taagcacctg ctgtattgat
     7981 caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta aagcttttcg
     8041 atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca gatcccgtcc
     8101 tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc caccaacaat
     8161 cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat cgaagatctc
     8221 ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa cgacatcatg
     8281 tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga agcgtatgga
     8341 ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga gataataaga
     8401 attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc
     8461 ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct tccattgcaa
     8521 aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa aaaagacctt
     8581 cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga gaaaagattc
     8641 cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga ctttcctttg
     8701 ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata cttcccatga
     8761 taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt gctttaccag
     8821 tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt tccgtatatt
     8881 cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca aaactttttt
     8941 catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat ccgcaatgag
     9001 ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa tttggacact
     9061 ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct ctattaggat
     9121 tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct atctccgttt
     9181 ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta
     9241 tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg attccttctg
     9301 tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc gtatttccat
     9361 ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat cccgggtaaa
     9421 ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt tggatcttcc
     9481 ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat tcgattgcgt
     9541 gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct gctatctcca
     9601 cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt tccacttgga
     9661 ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga aactcgatcg
     9721 ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct cctagtgctt
     9781 ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt tgattgttgc
     9841 gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga tcacctaaga
     9901 tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata tcatcatttc
     9961 caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg
    10021 attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc atcactggat
    10081 aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca ccagtacttc
    10141 ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc ttatttccca
    10201 ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct tcacacatgg
    10261 aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct tcggcttttg
    10321 tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg gcttttgccg
    10381 ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca tctgcttctg
    10441 aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata cgtacagcga
    10501 tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc aatcataaag
    10561 gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt gcctattccg
    10621 gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc cggtgtaact
    10681 ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc cttgaattgt
    10741 tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat caatacccct
    10801 ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat cgttacattt
    10861 ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat caaaagcatt
    10921 ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt ccctctttcc
    10981 tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac aactgactcg
    11041 ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc attcatcagt
    11101 ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc tacttttttg
    11161 aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg cgaaacaacc
    11221 tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc agttgttgct
    11281 tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt ttgaaccata
    11341 ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc ttcacgcaaa
    11401 tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat ttcattgata
    11461 tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg ccaaacccta
    11521 atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg acaactgtca
    11581 agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat tgatgttcta
    11641 atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa tgtccagtca
    11701 tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg atttggattg
    11761 cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg atatatccga
    11821 cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta gatgcaccag
    11881 aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg tatccaacga
    11941 tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca agaactagaa
    12001 cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga atcgaaccac
    12061 gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca atccccatct
    12121 cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca tgtttgatgt
    12181 tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg atagctgtac
    12241 aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca
    12301 ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga ataacttggt
    12361 tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac cggtcttgat
    12421 tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg tagattccat
    12481 agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta tcgtaaggga
    12541 aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg
    12601 gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa
    12661 taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg ctctcctttt
    12721 cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg atatatggat
    12781 tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg
    12841 gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc atatcagttg
    12901 tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct tctcgtgcca
    12961 cagattttgt taatcctact acgccagctt tactagctgc ataatttgct tgaccaatat
    13021 ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc tgcttcatca
    13081 tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata tccagacatt
    13141 ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt ccggcattgt
    13201 ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt tgtcccgctt
    13261 tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat gcttcgattt
    13321 cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt gctccagctt
    13381 ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg ataaatacat
    13441 tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt catcaaatgt
    13501 ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca taaatccgct
    13561 taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt ttaacttatg
    13621 gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc ccgcgattgt
    13681 ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa
    13741 ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg
    13801 aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt ttaatagtaa
    13861 aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag gtgtattata
    13921 atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct cgatcgtttc
    13981 aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag cttctgtcat
    14041 ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg cattactagc
    14101 tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga tgattccatg
    14161 ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct gtgtatattg
    14221 cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt agccgagaat
    14281 ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat cttttcccat
    14341 accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt ttttctccaa
    14401 tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat tcctatttca
    14461 ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt tctacgtaag
    14521 tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg tcaaattcat
    14581 cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag tcttcttgga
    14641 tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg atgatttctt
    14701 gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt ttatttttat
    14761 aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc gccatagcct
    14821 gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac tgcttcatcc
    14881 aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt ggtcaggaac
    14941 ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag gtttgcctga
    15001 tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt gtttgcaacg
    15061 tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata cgaaccttct
    15121 gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa tgagctactt
    15181 ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt cgcttctaag
    15241 agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga ccaatcgatt
    15301 agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca gcggatcatt
    15361 ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat atcaaaagcg
    15421 atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa
    15481 tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag atttttctgt
    15541 tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt tgttacacat
    15601 cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc catcatcttg
    15661 gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc agtaatcatt
    15721 ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg caagtttttc
    15781 agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat gttcttgacc
    15841 atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt cgttagtccc
    15901 aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc tttttttact
    15961 aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt ggctacttct
    16021 gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc tgtgattgac
    16081 agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat atcattgaag
    16141 acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca ccgtccacta
    16201 attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt ttcaaaatgc
    16261 aaccttaaaa gatcattttt ttactatatg agcttttttt aagattttac tcatttaaaa
    16321 caactgataa atcagcattt ataatttctt ttattagaaa aatcatctat ctcacatatt
    16381 ttgattatca aagtattttc tgtgaacaga actatagaat tccctaaaac ttgtccatta
    16441 tttcaaaatt tgttatagtc tttttattca aagcgtaaaa ggagatccta tgttaaaaga
    16501 aaaaatgaaa actctcccca tgctgccggg tgtctatctt atgaaagacc gagatggtaa
    16561 ggtcatctat gttggtaaag caaaacggct caaaaataga gtaagcagct attttcatca
    16621 gaataaacag cattcaaaaa aagtattacg tatgattcat catataacag attttgattt
    16681 cgtcgtagta gacacggaat tagatgcttt attgctcgag tgtcaattga tccaacatta
    16741 ccgtcctttt tataaccgac aaatgaatta tttcagtaat tataattatg tacatattac
    16801 caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc cttttcgtct
    16861 ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa tgccttggtt
    16921 gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg aaatgacttt
    16981 tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa agctactgac
    17041 ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa aagccggtat
    17101 gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg aacaaaagca
    17161 gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg agagtcagaa
    17221 aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct ctggtgacgt
    17281 ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac gtcaattgtc
    17341 aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat tagaaaaaga
    17401 acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa attagttgct
    17461 tctcggcttc ttttaggcat cttcaacttc aaatgtcaat tatcctaaaa tttattggat
    17521 taagtcatag atttccatcg ctacgatatc gatattatca aattcgtatt gctgatgagt
    17581 atctgtttca gttaattcaa atgtatcggt cgctttatca taagttactg tacacttctc
    17641 tacgccttct ttttcaaaac ggcgaacttg cagttcatta tctgctgact cgaccatagc
    17701 ctccagtcgt tttatgattg cgactaattg tgaatgtgtc atcggaggtc ctccttttct
    17761 tatttctgtt atattgtatc aaaagcatag cctacttgca ttgattttct ctaaaaaaac
    17821 tcgtttttta tgattttttt tctggtattt ttccttcagg ccaccaaagt ttgatcaaag
    17881 cttgggttcc gtcattctca aaacctttat ctgccaactc ttcatatagt ttcaatgctt
    17941 gggtagttgc tggcaataca agatccattt tttctgcttc ttctaatgca atctttaaat
    18001 ctttgataaa atgtttgaca aaaaaaccag gtgtgtaatc ttcttttaaa atacgaggag
    18061 aataattgct taaagaccag ttagctgcac ttccacctga aagcgtctca atcactttct
    18121 ttaaatcaag tccgtttttt tgtgcataga caagcatttc cgtcattcct gtcatcgtac
    18181 ctgcaatcat gatctgatta gccatttttg tatgctgacc tttcccagca gaaccatgca
    18241 atgtaaatgt cgtaccaaat tctttgaata aaggcagtac ttgatcataa acggcttctt
    18301 ctccaccgac catgatcgtc agtgtaccat tctttgctcc taagtcacca ccggataccg
    18361 gtgcatctaa cgcatctgcg cctttttctt ttgctgtttt tgcaattttt tctgccaggc
    18421 ttggtgtact tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca gaaaaaatcc
    18481 ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt gtaaagagaa
    18541 tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct tctgttacta
    18601 aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct gcttctagta
    18661 gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt ttcatttttt
    18721 cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga aacaaggatt
    18781 ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat tttgattcgt
    18841 tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac aaccatgtct
    18901 tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata aagtttagct
    18961 tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag tgctggtgca
    19021 aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa tcggttcaaa
    19081 taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt ataagtaagc
    19141 ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata ataaagtgct
    19201 acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg cagttctgag
    19261 atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa tctggcgtta
    19321 ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt tttctcataa
    19381 gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag tgactccctt
    19441 aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat gtcgatacca
    19501 gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc aataacacgt
    19561 tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa gtattttaca
    19621 tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc atacatcgta
    19681 tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc aagtatccct
    19741 gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc aaccaaataa
    19801 gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa tggtacactt
    19861 ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt tgtaaaaata
    19921 tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga tgtagcaact
    19981 ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt tttcgcctcc
    20041 aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt cattcagtac
    20101 actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct cttttgctgt
    20161 ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt ggatcagtcg
    20221 gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta accagccatt
    20281 atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt ctaaaacaac
    20341 gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg aatacgcacc
    20401 ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat gatcttcttt
    20461 tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa accaaataaa
    20521 tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt tacgttcgaa
    20581 aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat attctctgaa
    20641 cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat tggaaatagc
    20701 ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag gtgttggcgt
    20761 gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga taggagaaag
    20821 ctggttcgat ctgataaagg aatttcggaa atcttcctct gtagaatgcg catgatagac
    20881 aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga ttccgtatgt
    20941 attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga taccgacttc
    21001 cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg caagcatctt
    21061 ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta tatagtataa
    21121 catagcctac aaactgtcac tcaaacttaa gccctatttt tgtctattct tactgacaaa
    21181 aacgttttct taaaaaagaa caaaaaaact ggcgtagctc ttttacaagc cacaccagct
    21241 tttgatacgt tgtttatttt acatattcat gaacaagttc aacgacttct tccatcatat
    21301 cgcattcttt caatgcacga tcagcaagtt cagccatttt cgctgtgtcc aagcgtttca
    21361 ttaaactacg cgttttaaga atagatgtcg cactcattga gaactcatct agacccatac
    21421 ctactagcaa tggaacagct gtttgatctc cagccatttc tccacacata cctgcccatt
    21481 ttccttcagc gtgtgctgca tcaattacat ttttgatcaa acgtaaaatt gatgggttat
    21541 atggttggta caagtaagaa acgcgttcgt tcatacggtc tgctgccatt gtgtattgaa
    21601 tcaaatcgtt cgtacctaca gagaagaaat ctacttcttt tgcaaacttg tctgctaaga
    21661 ctgctgctgc tggaatctcg atcatgatgc cgacttggat cgtatcagat acttccttgc
    21721 cttcacttat caatttttgt ttttcttctt caaagatcgc ttttgctgcg cggaattctt
    21781 tgagtgtagc taccataggg aacatgatac gtaaattacc atgaacagat gcacgaagca
    21841 atgcacgcat ttgtgtacgg aacatttcgt cgccttgttc agataaactg atacgcaatg
    21901 cacgatatcc taagaatgga ttcatttcat gaggtaattg cagatatggt agttctttat
    21961 ctccaccgat gtccattgta cgaacaacta caggttttcc ttccatacct tctaatacag
    22021 ctttgtaagc ttcatattgg tcatcttctg ttgggaagtc tggagaatcc atatacagga
    22081 attcagtacg gtacagacca actgcttctc caccattatt atggacacca actagatctt
    22141 ttggtgttcc gatgtttgca gctaattcaa aatgcttgcc gtcagctgtg acagtttctg
    22201 cattttttaa tttttcccat tctgcttttt gtgctgcgta atctgcacct gctttttcaa
    22261 attcagcttt ttgttcatct gtaggatcga tgattacatc gccttcgatc ccgttgacag
    22321 ccaaaatatc gccttctttg actttagcag taatttcttt tgtcccaacg attgctggaa
    22381 tttctaaaga acgtgccatg atagcagaat gtgaagtacg tccaccaatg tctgttacaa
    22441 acgcttttac aaaattgcga tctaattgtg ctgtatcact tggagtcaag tcatgtgcaa
    22501 caacgaccac ttcttcgttg atcattgatg gatttggtaa agtaacgcct aataagtgcg
    22561 ctaaaatacg tttagcaacg tcacgaatat ctgcagcacg ctcttgcata taagcattat
    22621 cttccattgc ttcgaacata ccgatataca tatccgttac ttcttttagc gctgattcag
    22681 catttacgct gttgtcttta atattttgct tgatttgacc taccatttct ggatctgaaa
    22741 gaaccatcaa atgggcatca aatacttgcg cttctgcttc acctaagctt tgcgctgctt
    22801 tttcacgaat ttgctgcaat tcttcagtag atttagctaa tgcagcatcc aaacgagctt
    22861 cttcagccgc agtatcttct actgttgcct tactgaatgt taaatccggt tgaactagca
    22921 ggtaagcttt tgcaacggct acaccgtcac tagcggcaat cccttttagc atttcaacca
    22981 ttattcagat aagccttctt tcttcattgt ttcaacgatt gctgccattg catcagcttc
    23041 gtcagcacct tctgcagtaa tagtaacatc agaaccttga cctacaccta aagacataac
    23101 gcccatgatt gatttcaagt ttactgattt acctttatac tctaagttta catcagagtt
    23161 aaatttgctt gctgtttgta ctaatagtgt agctggacga gcgtggatcc cagtttctgc
    23221 tactacgtga aattctttct tttccatatt aatcggtctc ctttaagtga gtgttgtttt
    23281 tatgtgaggg ttttcttttt tagtaattca gagaatccta acaaatgcca ccctttcatc
    23341 tgtaaagatt aacatttttt ttcgttagat acaacagttt tcattcaatt tcagataaaa
    23401 gtattcaaat tattccggat tctttttcat ccttttcttc acattttata acaaacgtgt
    23461 ttatttcctt tttcttgtcg ctttctcttc tcctgacgtt tatcattctt tcctttgacc
    23521 ctctttgttt tcatatgaaa cgaattactt gcttcccttt ttatttttag tataataaga
    23581 gtcaaaggtc aggaatggtc aatagagatt tcaccttttc tacctcataa atctcttaaa
    23641 aaactagggg ttattctttt acctcatgaa aaacgagatg taaaatatgg atgtacccat
    23701 ttttgtgaaa ggacgtgaat gattcatgct ttgtcaaaac tgcggcaaga atgaggcaac
    23761 gattcacttg tatgccactg tcaatggtca gcggacacaa ctcgattact gtcagagctg
    23821 ttatcaaaaa atcaaaaatc aacaaaatgg aggtctgact aatatggccc aaaatgatcc
    23881 atttggattt ggcagtttgg acgatctttt ccgttccatg tctcgccaaa tgcaacagca
    23941 aggaaattat gagcaaacac caccaacaca atttggcgga aacaacaact tcaatggtgg
    24001 tcagccacct caaggcaata cagaaggttt attgggagaa tacggaatca atatcacaga
    24061 acaagcacga caaggtgata tcgatcctgt gatcggacgc gatgacgaaa tcaaacgagt
    24121 cattgaaatt ttaaatcgcc gaaccaagaa caatcctgtc ttgattggag aaccaggtgt
    24181 tggtaaaaca gcagttgttg aaggtttagc acaaaaaatc gtagatggtg atgtaccgca
    24241 aaaattaatg gacaaagaag tcattcgttt agatgtcgta tctttagtcc aaggtactgg
    24301 tatacgagga caatttgaag aacgtatgca aaaattgatt gaagagatcc gtcaagcaga
    24361 aaatgtgatc ctattcatag atgaagttca cgaaatcgtc ggtgctggtt ctgctggtga
    24421 tggaaatatg gatgctggta atattttaaa accagcttta gctcgaggag aattgcaaat
    24481 ggttggtgct actaccttaa atgagtaccg catcatcgaa aaagacgctg ctttagaacg
    24541 tcgtatgcaa cctgtacggg tagatgaacc aactgtcgaa gaaacgatca gtattttgaa
    24601 aggtttgcaa aaacgttatg aagactatca tcatgtaaaa tatactgatg aggccatcga
    24661 agcagccgca actttatcca atcgttacgt ccaagatcgc ttcttgccgg ataaagccat
    24721 cgatttgcta gatgaaacag gttctaagaa aaacttgacg atccaaatcg ttgatcctaa
    24781 aacaatcgag aaaaaactgc aagaagcaga agaacaaaaa gttttagctt ctcgagaaga
    24841 agatttcgaa aaagcagctt attatcgcga tcagatcaat aaacttcaaa aaatgaaaga
    24901 acgtcagctc actgaagagg aaacacctgt gattaccgaa aaagatatgg aaaaaatcgt
    24961 tgaacaacgg acaggtattc cagtgggtga attaaaagaa aaagaacaga cacaactgaa
    25021 aaacttagca gatgacttga aagctcatgt catcggacaa gataacgctg tagatcgagt
    25081 agcgaaagcg attcgccgaa accgtgttgg attaaacaaa cagaatcgtc caatcggctc
    25141 cttcttattt gttggaccaa ctggtgtagg taaaacagag cttgcgaaac aacttgccta
    25201 cgaactattc ggttcacaag attcaatgat tcgtttcgat atgtctgaat atatggaaaa
    25261 acatagtgtc tctaaattga ttggttctcc tccaggttat gtcggatacg aagaagctgg
    25321 tcaattaaca gaaaaagtac ggagaaatcc ttacagtctt gtcttgttag atgaagtaga
    25381 aaaagctcat ccagatgttt tgcatatgtt cttgcaaatc ttagatgatg gtcgattgac
    25441 agatgcacaa ggaagaacgg tcagctttaa agatacgatc atcattatga caagtaatgc
    25501 cggaactggt aaagtagaag caaacgttgg ttttggtgcg gcacgcgaag gtgttactcg
    25561 ttctgtattg aaccaattaa acaactactt tacaccagaa tttctgaacc gttttgacgg
    25621 gatcatcgaa ttcagtgcat tatcaaaaga aaacttgatg acaatcgtca cactaatgct
    25681 agacgatgtc aaccaaatgc tggcagcaca gcaacttcac atcgaagttc ctacgaatgt
    25741 caaagaaaaa ttagttgact taggctatga tccatctatg ggtgctcgtc ctcttcgacg
    25801 cacgatccaa gaacaaatcg aagatggtat cgctgaattt tatcttgatc acccttctat
    25861 ccatgagttg aaagcaaaac tggataaaga tggaaaaatc atcgttactt ctaaaccaga
    25921 acgtttggct aaagaatcag ccgaagaaac ggctgaataa atccagtttt atagcgtgag
    25981 acggtatttc tttgagaaat gccgtctcat tcttttttca gaaaagtcaa aatactgttc
    26041 attttataaa aaagaaatta taataagaga aacaagcaat taaaaataca taaaagaggt
    26101 gagcgccatg aaattggtaa atgtaacaaa cagccattcc cgtcttgtat taaaccagct
    26161 tgaaaacact gatgcccatc tagtcaaggt ctacacagcc ggaaatacca ctattgtcta
    26221 cactgaagcc cctgaacata atgaaattct gctgatcaat gacaaacgta aaatccagcc
    26281 gaaagaaatc gaagatgcta aaaattattt cttagataaa atcaaagacg ccgtttacca
    26341 cgaggatgaa atcaaagtaa tcgaattggc aggccttgtt gaaatctcga ttccaaaaca
    26401 tgaattaggc gctacattag ccgtgtaaac aaattttgaa cataataatt aaagagcgtg
    26461 ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcgaaagcag
    26521 aagcaactcc ttcggaaata agctgaaatt cacgaaaatt gcttttcaaa tttttgtgaa
    26581 ttccagctta tttcttggag ttaaacgctt ctgtctcagc ctctttttct ttctagcttt
    26641 ttgatttttc tcttttcttt tgtacttttc tcttttcatt ttcagttcta cgcgtgtctt
    26701 tatttttaac tttttcctct ttatggatga ttgatcgtac tttttgtgca atttgctcgt
    26761 ttttgtccca gtcttttaca tctaataatc gacataatcg taacatctct tcttttttct
    26821 gtttttttct ttcacgtctt tgccattctg tttccatgtg tcacactctc ctttctttaa
    26881 tcatatgtac gcaaaataat ttttatattt ttttgattag acatttctat gattttaata
    26941 tagtaaaaag agccgtcacg gaaaatccgt cacggctcta aacaaaatat gagaggctgt
    27001 ttttgtttgg ctattcttat tacttgatgc agggcagctt gtgtcaagac ttctttacat
    27061 caaacttttt aattttacat tgggatattt atccgcaaac cagcgttctg caaattggtt
    27121 ctcgaataag aacaacggtt ggtcgaaacg gtcacgagcc aagatattgc gacttgaact
    27181 catcttctca tccaaatctt ctggatcgat ccagcgagcg attttgtgtc ccattggaga
    27241 catgattact tcggcgttat actcatttaa catacgatat tggaatactt cgaattgtag
    27301 ctgaccaaca gctccaatga tatattcttc cgttagatat gttttataaa gctgaatcgc
    27361 tccttcttga actaattgat agattccttt gtggaaggat ttttgtttca tgacattttt
    27421 tgcagtcact ttcataaaca gctctggagt aaatgaaggg agttcttcat aagcaacttt
    27481 tagttttcct tcgaacaatg tatccccaat ctgataattg cctgtatcat atacaccgat
    27541 gatatcgcct gctactgctt tttcgacgtt ttctctcgaa tctgccataa attgtgtgac
    27601 attgcttaat ttcagttttt tatttgtacg ttccaaccag acatccattc ctcgttcgaa
    27661 tgttccagaa cagattcgga caaaagcgat tcggtcacga tgggctggat tcatatttgc
    27721 ttggatcttg aatacgaaac ctgaaaattc ttcctcataa ggacttacca tctgatcatc
    27781 ttgtgttttg tgtccataag gagctggtgc caattcaacg aaagtctcta aaaaagtctg
    27841 tacaccgaag ttagtcaatg cagaaccaaa aaatactggt gtctgatcgc cacgagcaat
    27901 tttttcttga ttaaatgcat ctccagcctc tttgattagt tctacttctt ctaagacttg
    27961 ttcatataag gaattttcag ttagaggatg attttcggga agcagtccat cttcatccaa
    28021 ttctactaat cgttcatcct gataattttc cggacgataa aactctacac gattatggta
    28081 gatatcataa agaccttcta gccctttacc cataccgatt ggccaattca tcggataaga
    28141 ttcgatatct aatagctctt ctaattcttc taacaattcc aatggttcac gaccatcacg
    28201 atctagtttg ttgataaaag taaatatcgg gatgccacgt ttttttacaa cttggaataa
    28261 tttctttgtc tgtgcttcga tcccttttgc actatcgatt accatgaccg cactatccac
    28321 ggccatcaaa gtacgataag tatcttcaga aaaatcttca tgtcctggtg tatccaagat
    28381 atttactcgt ttcccttgat aatcaaactg cataacagaa ctagtaacag aaatcccacg
    28441 ttgtttctcg atttccatcc agtctgattt ggcaaaatta cctgtttttt tgccttttac
    28501 ggtcccggct tgacggatag ccccaccaaa caatagtaat tgttctgtaa tcgttgtttt
    28561 cccagcatcc ggatgggaaa tgatcgcaaa agttctgcga ttgtccactt gttgctttaa
    28621 ttttggatta ttcataacct aactccattt cttccattca attatttata ttttctctag
    28681 gcacagattg tccgatctct taaagaaaga caatttcata tagggctgtg acaaaagccg
    28741 tgtagcagtc tctttttccg agtaaacggc gttctagaga cagcctatca gcaataagcc
    28801 cacccaagcc aaaaatacga aaagctgttt ttgcttggct gctcttatta cttaatgcag
    28861 gatagctttt tcacaacctt tgtcaactac tacaactgaa tcagtatagc atggattcag
    28921 cgaatgaaaa agccaccaaa ccataagagt caaatatttt tttgtcagct gactaaaaga
    28981 aaagacgtgt tgcttgttta ttcaagcaac acgtctttca taaccaagtt tccaattatc
    29041 ttcttggctc gtcatcttcg aattcttttc tgaagtatct tcgttcttct tcaggttccg
    29101 cttctgtttc ttccggatca tgaaaaatga cacggatttt aaggattcga gaaccttcca
    29161 tttcttcaga aaccagcgta atattatcga catcaaacga taacttttcg ccttcatccg
    29221 gaatcatgcc taatgctgta atcaaataac cagccatcgt atctacatca ctcatatgca
    29281 gatcactgtc aaaagcttca ttgaactcat caatcagcat acgtccttgg atgatatatt
    29341 cgtgttcatc gattttttca taaagattct caacttcatc tgattcatca tcgatttctc
    29401 cgacgatttc ttctaaaagg tcttctagtg ttgctaaccc aacgacgccg ccatattcat
    29461 ctaataaaat agccatctga ttctgcgtct ttttcatttc atataacaga tcatcaataa
    29521 agaccgtttc aggaacgaat aaaggctcct gcatgatttt cttgatatct aaattatcaa
    29581 agccgaattt atgggcagct tttagtaaat tctttgtatg aaggattccg actactttat
    29641 ctttgtcttc gttataaaca gggattcttg aatagttttc ggataaaact tcattcacat
    29701 tttcttccac agtgtcgttg atatcaatca taaatgcatc cgttcgtggg accatgactt
    29761 ctcgggcaac tttcgtatca agagaaaaaa ccccttggag catttctaat tcttcattat
    29821 tcaatacgcc ttcgttttca agcatataac gcatttcatc tctggtcatt tttgaatctt
    29881 catcatcaaa agtcatcggc gtgatacgcg ccaataaact tgtggaagca gaaagcagcc
    29941 agacgaaggg cttagctact acaccaatca agcgtatcag ccctgaagtg aactttgcga
    30001 cttcttctga tttatttaaa gcaatacgtt tagggtacaa ttctccaaaa acaatcgata
    30061 cataggtcaa aatagccagc acaatgatat ttgcaatatt ttttgccgct gtgccgccac
    30121 ctaataccgg agctaaccgt tccgataaag tattagctag tgaagcacca gacaaaatgt
    30181 tgaccaaagt gattcctact tggattgtcg ataggaaatt atttggattc tgcaatattt
    30241 ttaaaagctt ttgagatttc acatctcctt catcagcttt ttgctcgatt cgattcttat
    30301 tgatcgaaac aacagcaatt tcggaagctg ctaaaaaagc attgatcaat gtcaaaacga
    30361 tcaataataa aatctgcgct aacagcgact gactctcagg gtcagcattc ataatcgcat
    30421 tctctcctta taaaattaat ttttagttta cgatagaaaa aaacgtggca ctatagccac
    30481 gttaaaatgg tatcacaaag caattttctt tgcaataaaa acaagtgtac taaaactacc
    30541 tacgaggtcg tagcaatttt cttcttgttt tcattttgct tgtcttcttt gacgattttg
    30601 attatatagg aaatgatcgt cttgcagatt gcgtaaaaag gaacacctaa gaagatacca
    30661 agcaatccgg caatatttcc agctactaga agaacgataa tgatcgttaa tggatggatt
    30721 ttcagtgatt tcccaattac attaggataa atgatattcc catccaactg ttggacgatt
    30781 agcacaacca cacaacaaag taatgctttt accggatcat tgaagactgt tactagaaat
    30841 gcaggtgcta gtcctagata aggtcctaaa tatggaatta ggtttgtcaa tcccgcaatg
    30901 accccaaata agaaagcata atccacacca attaccatat atcctaaaaa tgtgaaggta
    30961 ccaacgaata aacattcgat tgcttgtccg ctaatgtaat tagccaatgt tttattcagt
    31021 tgccccaata actccacaat ttggtttctt cttttttctg ggaaaaactg ttggatgttt
    31081 ggcactagtt tttctccgtc tttcaacata tagaacaaga tgaatggtgc tgtaatgatt
    31141 actacggttg ccgaagcgat cgttccaacg atcgaaccta aactattgga tactccacta
    31201 aggaattgct gaatgattgt cccgtaagaa atgtctaatt gctcaaaata tttattgata
    31261 tctacttcct tgaacaaagg caattcggct acctctttta accagctttc tacctgtttg
    31321 atgaaatcag gcatattaga tgccaatgag gaaatttgct gtactagact tgggatgaca
    31381 ctaagtaaaa tccaaataat tgctgcaact aaaaggagca gcacaattgc gacagcccat
    31441 attcttttga tttttgcttt catcaacagc ttgacaatag gattcaataa atagtaaaga
    31501 aagcctgcaa tcagtacagg tgcaaataac gtggaaaaaa atgtcccgat cggttcgaaa
    31561 ataaagttta tttctgatga aatcatgatc aatgcagcta tgatcaataa ttcaagcgac
    31621 caaaacatta gtttagactg tctaagtttt tcaaacaata gatttcctcc tttctttctt
    31681 actagtatat attatattcg ttaaaaattc ttttattctt tttttatttt tgaaaacttg
    31741 ccatactttt tatttgtttt attttccagt atactatacg aaaggaaggt ggctagacca
    31801 tgaaagttgt acaaacaaaa gatacaatga gcaatattta cttagatgct gtacggatca
    31861 ggcatcaagt ttttgtagtc gaacagggtg tgcctttgtc tcgagaaata gacaaagatg
    31921 aagcccattg tatccacttc gtcctgtact cagataaaaa agaaccgcaa ggaactgtac
    31981 gtcttttgcc tttagaaaac ggaaaaatga aattacagcg gatggcgatt ctttctgaat
    32041 acagacacca agggttagga aaaatattga ttgaagaagc agaaaacttt gctaagaatc
    32101 aaggctataa tacgatttta ttaggggcac agtccacagc tgaaactttt tacgaaaaac
    32161 taggatatac agcttacggc gatccttttg aagatgctgg catgcctcat atttatatga
    32221 aaaagacttt ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa
    32281 tctctcactg gcttactgtt cttttttgtg tttacttacg cgacaattgc ttgtaacggt
    32341 tataccaaat atcaatatag ccttccgaga acgggccatc tccattattg atccagtcaa
    32401 ccagaatctt cacattttct tttaggatga agtcaatatc gttcgggtaa ttcatcattt
    32461 tgctgtgcat ctcatattca tcgacatcca atagtcgttt ctcgccgtca ggaaatacct
    32521 tgatatccaa gtcatagtcg atatatttca aggcctcatc gtccagcaaa aaaggtgaag
    32581 ctagattaca ataataggaa accccctttt ctctgatcat tgctattaca ttgaaccagt
    32641 attttttatg aaaataaaca atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt
    32701 cagtgactaa tgtgtgatca ttcacgccga tcatagagtg ttcgcttgtt tttaatacca
    32761 tggtatctcg ccacgttcga tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa
    32821 actctccttc ttttggaact cccatagcaa cccttcctta agcagcattt tgtgctttgt
    32881 gtttgggtgt tcattacacc gacatcataa ttattatatc aataaaaatg agctttgcct
    32941 agaaaggatt cctattgttt cgatttttct ctaactgatc taccagtttc atttggacct
    33001 taggaaaaac gaccgaatca aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg
    33061 taaattcttg ttctgctccc tcggttgccc gtccgtaaaa aaggagaaca tgccatttca
    33121 gatgactaaa aatatgagta acttctccta aatgtcgtgt ctgccaaaca aagagttcct
    33181 gcttttcaaa tgggagattt tgctcttctt ctgctactag gtcatcaaac agatcgattt
    33241 ctggctttgt ttcccattct tttttcaatc gctcatactc ctcttgtgtc acttccatca
    33301 ttggaaatgt ccacatattc gccagcaatt tttggctatc tctttcttca aaataatacg
    33361 ctccagaatg attttgaaga gctgcactga tataataaac gtcttttggc tttgcttttt
    33421 ttgtttttac aggaaaactc gtttggattc ctcgtttgtt agctaaacaa aaggcttgga
    33481 tcggacaagt ctcacacttt ggagaagttg gtgtacagat agctgaacct agatccatca
    33541 ttgcttggtt gaattcacct ggatgctttt catcaatgat tttccgcatc gcttcatcaa
    33601 agattttcct gctagaagct tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta
    33661 ctcgcatcac gttgccgtca actgcaggtt ctggaagtcc aaatgcgata cttgcaatcg
    33721 cgcctgtcgt atatggtcct attcccttca atgagctaat ttcttcaggc gtttgcggca
    33781 tttccccatc aaattctgac atgatctgtt tagcagcagc ttggatgttc cgggctcttg
    33841 agtaataacc aaggccctcc catgctttta aaagcttttc ttctggagca tttgctagct
    33901 cttcaattgt aggaaaccat tccatgaaac gataaaaata atcgatcact gtgtctactc
    33961 tggtttgctg aagcatgatc tcagagatcc agatacgata aggatcacga ttatagcgcc
    34021 aaggcaggtt tcttttttct tgttcatacc attgaatgaa ctgatcttga aattcttttg
    34081 tttctttatc tgtccatttt tcccattctt tattcacttt cctcttcctt ttccgtaatg
    34141 aatcgctgga tcatatccaa atcaaatcct ttttgatata agctttgctt tacttttaga
    34201 tttctttttt taggttcaaa ccttaaattt cttcgccata gtttatctcc ttgtttgact
    34261 aaataatcat attctatttc ttgatctgct tcttgcggaa gatcagctat cgcttgttgg
    34321 atcacttctt gagaaaagcc tttcgtcatc aatgtttgtt ggatttttct caataattct
    34381 ttttggcttt ttccgtgatt ttttccaaac gctttttcgg ctgtctgatg tgcattttga
    34441 acttggtctt caaatgtata ttggctgagc gcttgttcaa tgatttcagg tttcaaacct
    34501 ttttgctgca tctgctgcgc taatttcttt ggtcctttgt cacttaagcg catattcgtg
    34561 cgcacataac tttccccata gaccaaatca tcgacaaggt ccaattcttt taagcgggct
    34621 acgattttat ttcgatcttc ttgaaggatt tccttttctt ttaagtatgt tcggatttct
    34681 ttttccgatc gcaactgata acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct
    34741 ccgctactct tcttgatctc ttggatagtt tcttcgtcta attcccgtcc ttttaataat
    34801 cgatagcgta ctaatacatc ttcagatact tgtaaagctt ctccttcaga taaatctacg
    34861 cgatagaaag ggcctttgcc tttactgatt ctcgtaacgg ttaacataaa catcctcctc
    34921 gaacatatat tctctatcat accacagaaa atccccgtaa tcaggtatta tatgttagaa
    34981 taaaccacag aaaaaagaaa aagggagata cttatggcag aaacaattgt caaaactgga
    35041 caaacggttc ctttgaaaat caagcgttta ggcataaatg gtgaaggaat cggttatttc
    35101 aaacggttga tcatatttgt tccatatgca ctaccaaaag aagaagtact agtgaaaatc
    35161 acgaaagcaa ctcctcgcta tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa
    35221 gatcgcgtag tggcaccatg tcctgtttac tatgaatgcg gtggctgtca attacaacac
    35281 ttagcttatc aagctcaatt ggattttaaa aaggatctgc ttttgcaagc attggaaaaa
    35341 ttcaaaccag caggctttcg atcttatcaa cttttgccga cgatcgggat ggatgagcct
    35401 tggcattata gaaacaaagc acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt
    35461 gaagctggtt tatacgaagc aaactctcat cagttagtcc ctataacaga ctgcttggtt
    35521 caagaacctc gaacacaaaa agtaatgaat actgttgttc aactattgaa taaatttgat
    35581 gcacccatct atgatgaacg gacaaacagt ggaatcttcc gcacaatcat ggttagaatc
    35641 ggtgttaaaa ctggggagtt acaagttgtt tttattactc gcagtaaaaa attcccgcaa
    35701 aaaaacgcaa tgatccgtga gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa
    35761 aatgtccagg cacaaaaaac gtctctcgtg atgggggatg aaacgatcca cttatgggga
    35821 aaagaagcca ttgaagaaca aatcaatgaa gtgactttcg atctttctcc tcgtgccttc
    35881 tttcaactca atcctcagca gaccgaattg ctttatggcg aaggaatcaa agcattagat
    35941 gtccagccta atgaaacgat cgtggatgct tattgcggag ttgggacgat cggcttaagt
    36001 gtagcaaaac aagccaaaga agtacgggga atggataccg ttcctcaagc aatcgctgat
    36061 gcaaaaaaaa atgcggaaag attaggatat cacaacactc gatatgaagt cgggacagca
    36121 gagcaagttc tgcctaaatg gttgaatgaa ggttttcagc ctgatggtat catcgtagat
    36181 ccaccacgta cgggtttgga taacgcattg atccaatcat tattgaaata ccctccaaaa
    36241 aaacttgttt atatctcctg taatgtttct acacttgcca gagatctggt taatttaagt
    36301 aaaaagttca agattgaata tttacagtcc gtcgacatgt ttcctcagac tgcacgttgt
    36361 gaagttgtcg tgaagatgac gagactcgct gactaaatga aagaggttgt gaaaaagtct
    36421 gccctgtatc aagtaataag aacagccaaa caaaaatagc tcttcatgtt ttatgaatac
    36481 tgtacttgtg accgaagagt taacaggagg acactattta tttaaaaaaa gggttgtgac
    36541 atagtttttg tcacagccct ttttgattat tattggatat tcacgtctat cacttgataa
    36601 aatgcatttg tcgtatcgct gatattccat actgctaaaa tcacattata tccttttcga
    36661 tcctgcggaa gtgtgatcgt ttggttgact gtttttggtg gaacagttgc tttgtcgtct
    36721 atttttgtga tcaattcaaa atcactaaat ttcaaaggtt gattaggatt ccaagttggt
    36781 ttggtaatgt agtaatccca agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata
    36841 tttaatgttc ctggattaac catgttttta tgccatctgt tagatgtctg ttcatctaaa
    36901 ggctcaaagc cgctgactcc tgcactagca atttttccat caatgaatgt atttttggta
    36961 gcttcgatac tttgcggttc ccattgtgct cttccgacat tttgatttaa gtttcctgct
    37021 gctgatgttc ctaggtatgc acggcttcct ggagaagtaa tataaccatg cgcaaaacct
    37081 ggaatgctaa aaccgaacaa ccctaaacta actaacccaa taaacaataa cgtactaatc
    37141 tttttcatgt agaaacctcc ttcatttgat aaccacattg taaacgcttt attgtattaa
    37201 cacaaacatt atgcttttat aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg
    37261 cagattataa cagaagtaga cgagaaaagt acaaatagaa aacatgagaa tcaaatcata
    37321 tgatcttttt caaaaacatc attcatataa acaaatgttc aactttttaa taacaacaat
    37381 tatcaatttt tttcttactt cacaaactaa atacaggtct tgctgtgagc cttcatggta
    37441 aactaatatt atagaaagga gttagtaaaa tgaagatgat aaaagatatt cgtttttggg
    37501 tttgtgttat aggaattgtc atattaggct ttttatcagg gttgcttagc ggaaatcccg
    37561 gaaaatatta ctattctttg caacttccac catttgcacc acctagctgg atatttgggc
    37621 cgatgtggac cttactgtat atattgatgg gtatttctct ttatttgtta cttaatcaca
    37681 acaacaaaaa acagcgaaac aatcttgtcg gtctttttgt gattcagttc atttttaatt
    37741 ttatatggtc tgctctattt tttaatttaa gaaatatttt cattgcagca atcgatatta
    37801 cacttttagt tattttcttg tcagtgctta tgtatcagtt gtggctgcat catcgcctgg
    37861 caatgtggct catgattcct tattatttat gggtactatt cgcaactcta ttgaattatt
    37921 caatttattt tttaaactaa gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa
    37981 agtgccacct aagttagatt ttttgtctaa tttaggtggc actctttatt aaccaaacta
    38041 tttttttcta gtacccactt gttgttccat actcattatt ctggtcataa ccataaccat
    38101 tttgttcctg tccagcgtaa ggatcataac tttgagtatc ttgacttggt gtatagtact
    38161 gacttgtatc ttctgtttgc tgataatagg tattcggatc ttggtactga tcatatgtgt
    38221 tttctgaata ggaagaataa tcggtgtacc cttcactatt cgatccatca ccgtttgtcg
    38281 aatcgccatc ttccgaatca tcaaagaaca tcattgaagc attctcattt ttcaaattag
    38341 gtaatgtatc gcttgttttt atatttaatt gtttcttcag ttcattttgg atcgaaagaa
    38401 gatcattttt tcctaaaatt tgatagtaga tatcattgat catttgtcct tcgccttgca
    38461 actggtcttg tttgattgtt tcaaacgccg gggtgtagtt cgtcgctaca tcaagcatgt
    38521 catcccaatc taagtccgtc gtcacattgt tctcaactgc ttttaaaatt tttctataat
    38581 tgcttacgcc atctaaactt aaaactttat tgacgatttt cgtcactact tcccgctgac
    38641 gacgttgtcg accgatatcg ccttctggat cttcgtgtcg cattcgagaa taggctaaac
    38701 ctttttctcc attcaatgtt tgttcaccta ctggtacatg gacaccatcc aaagtaaagt
    38761 caattttatt attcactttg atgccaccga ccgcattgat caaatcttcc atgccatcca
    38821 aattgatcgt tacataatga tcaataggaa tatcgagcag ttgctcaatt gaatccatgg
    38881 ccatttcgac accaccaaat gcgtaggcat gatttaattt atctaccgtt ccataaccta
    38941 cgatattcgt ataaatatct cggtccaaac tgatgatcgt tgattttttt tgtttcggat
    39001 taactgttac taccatcatc gtatcagaac gtccttgttc cgttcttcct aatccaccgg
    39061 tatctaaacc taatagcaaa accgaaaaag gttctttgtc atcaatgctt actctctcag
    39121 aacgtttgga ggatattcga ttagagtttt tagaaatttt gtttactgtt tggctcgctt
    39181 ctcccataaa tctgattccg tatgctgata atccggcaat cagcaaaacg aataccccta
    39241 acaaggttaa tagaactttt tgccatcgct tcataataca cctctgtttc acattttcta
    39301 aaatattgtc tcatataact gttcaatcag caaattcact ttttaatcat aagaaaactt
    39361 taaaaaacat ttttgtgtaa cgattattat tttttaaaat ttctcaaaat tcttaactat
    39421 cataccatac cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa tcgttttatc
    39481 tttttttatg tattttttcc agaaaaagcg aacaacaagg tattttccaa ataatcgtgc
    39541 agtaaaatga tcgcacatac ctttcccatc tttttaacag tctttgccta ccgttcagat
    39601 tatcattcga tttcacgaca atttcttcct atgactggtc cttttgttaa acccatcttc
    39661 tattcgtttt atttccagct cactgcatgg ctcttaaaat gactcgaact agtgattgca
    39721 ttaccatcat cttttaatgg atagatatgc tcctcttcaa agtaaaacga ttttctttta
    39781 aagtaataga attgacatgg taatgagtat gtatactagt ggtttcacaa atggtctttt
    39841 attcaacatc caaaaactga aatttcttta aaatccatta ctaacgaaga aaatgttcct
    39901 tgcttgataa ttagttagga catgtcaagt gaacaaatgc atttacttag ccaaaaaatc
    39961 taaaaacttc ataaatagac aaaaagacac gactctctaa gaagtcgtgt ctttattcaa
    40021 caaattatta taatttagtc acttcagcag cttgaggtcc gcgtgcacct tcaacaatcg
    40081 tgaattctac ttcttgacct tcttctaggc tcttgaagcc gtctccttga attgctgaga
    40141 aatgaacaaa tacgtcatct ccgccgtcta ctgtaataaa cccaaatcct ttttcattgt
    40201 tgaaccattt aaccgttccg tgttccataa atgtatatcc tccaaaaaag ttcttgcaag
    40261 taaccttgca agaacattat acccaattta gaaaacgcta gcaagctttc gctcttttat
    40321 tttccaaatt atttttaaga ctgatatgac ggaggaaatg gttattttgc tgcttcgaaa
    40381 cgtttgttta cttcatccca attaataaca ttccagaatg ctgcaatata atctggtcgg
    40441 acatttttat atttcaaata ataagcatgt tcccaaacgt ccaaacctag aattggtgtc
    40501 tttccttcca tcaatggaga atcttgattt gcagtagagg taatagctaa ttttccattt
    40561 tccattacaa gccaagccca tccagaacca aatcgtccag cggctgcttt tttgaattct
    40621 tctttaaaag aagaaaaatc accaaaagct tcattaatcg cttcttttat ttctcctgta
    40681 ggttcgccac cagcatttgg tgccatgatt tcccagaaaa atgaatggtt agcatgtccg
    40741 ccaccattgt tacgtacagc tgtcttgata tctgttggaa tagcgtccat atcagataat
    40801 aattcttcta ttgttttttc gcctaattct gggtatttct caattgctga atttaaattc
    40861 gtcacataag tattgtgatg cttatcatga tgcagatgca tcgtttcttc gtcaatataa
    40921 ggttccaatg cgtcatatgc ataaggtaga tctggtaatg tgtaagtcat aaaaaaattc
    40981 ctcctcaatg ttttcttaat aatgtctaca ttacaaagag taacaaagag aaggaaattc
    41041 ttcaattgtt atgcctagtc gtctttttca ctttgaccta cttcttcttc aaaaacctca
    41101 gtttctttaa aaataaacaa cttgctgaaa atatagttcg caaggataac aataatttga
    41161 gtgatgattt tcgccacata ctcattgctg ttcataacat tgatcaacaa taccattgcc
    41221 cccatgtcca atccgtaaga aaggatacgg tagaagaaga acttaccgca ttcaataagt
    41281 gcatgtgtaa aattcggtga ttttgaatgg aatacccaaa gtttgttcgt tacaaaagca
    41341 aacaatacag aacaaaacca cgaaatagaa ttggataagg ccatacttaa atcaaaatat
    41401 tggcgtgcga gagcaaaact aacaaaattt acaatcgtag ccaaaccacc aaaaaagaga
    41461 tagactagta tctcccaata tccttttgct tctaaatatc cttttaactg tcgatactgc
    41521 ttcatgagcc aacctacctt attaaaaatt cttatttatc atactctata tttcaataac
    41581 tttcatcttt atttaaaaaa attaattttt tttgatcctt tgcctttgtc tctgcttgga
    41641 tcgtcacatg tgtaatacca tattttaatt ttaataaaga aattatacga ggatatatct
    41701 tttcaatctc gcttagtagc tgatcttcaa aatcaatatg tccgtcgaaa ataattatct
    41761 tttcgctata ctgccaaata tggacatgat gaacatcctt taccccatcg attgcttcaa
    41821 tttcttgttc cattttttca tagtcgatag caggtgcact ttgtatcaaa atagcggcag
    41881 cttttttgat caccattatc gcttctatca atatatatat cgaaatgatc agtgtaataa
    41941 ctggatcgat ccagtagata tcgaaaaaac ggatcaaaat tgctccgata atcacactta
    42001 ccgaagacag tgtatcactt aacaaatgca aataactaga ttttatattc aaactttcat
    42061 ctgcttcttg tcgtaataat aatactgaga acaaattcgc gagcaatcca ataactgcta
    42121 ctgttagcat caaatgacta tttatctttt ctggtgaagt gaaccgcttg aatgcttcaa
    42181 cggttaaaaa caaggataat atgatcaata tgaatgagtt gatgaaagca ctcaatattt
    42241 ctaaacgctg gtagccatac gtacgttttt tatttgcttc ttttcgggcc attttttgtg
    42301 ccccgtaaga aaaaataatt gccaatgtat cactaaggtt atggaaagca tctgaaacaa
    42361 gagacaaact ccctgacata atccctccaa taaattctga taaagtgatc accgcattca
    42421 aaaccactac tccaaatagt ttttttgatg atctctgtgg ctgtgtaggc actattcctt
    42481 cctccatttt atttatattg gatagtcttt agacaagaat aacatttttt tgatatttca
    42541 ccaatcattc agctgtttcc ttaaagcact tgtatttcac ttgatacttc gctagaattg
    42601 caagtgatga cactactaga tgaggagtac ttatgacaac aaaacaatta attatcagtt
    42661 cctttatacg attcaaagac ctaaaggagg cgagaaatgt cccattttgg aaagccattg
    42721 tctatctttt agttttgagt ataattatgg ctttgccaat ctcttttcaa gtttttcaag
    42781 tcttaaacaa cattcaattg gatgggcaaa agattgctac tcgcattcct gattttacaa
    42841 tccaaaacgg gcagcttcaa accgaagaaa aagaaggatt catttaccaa acaaactcca
    42901 ttatcttcac atttgatcca gagggaaaac gaacagaaca ggacatctct actgatttga
    42961 tggggaattt tgttagtgtc gggatgttaa aaaacaaact tatcatagct cttcctaata
    43021 caggcacgac ttctgctttg ttgaacaata accaattaga acttccctac accaatgaat
    43081 ctttaaaaaa cttaacaggt aagcagctcc gttctttttt aagtgaagca tctataccac
    43141 tttggataaa agtcctgaca tttatatttt ctatttatcc tagttttctg aatctgctga
    43201 tcactttgct atttaccaac attgctgctt ttctctatgc acgctttcga ctaacaaaag
    43261 ctacttttct tgattgttta aaaacattga tctattcaat ttcattgcct actataattg
    43321 caacaatctt gatggtcttt cttccttctt ttgacactag cgccttcatt gcttttgcag
    43381 gaatctttat ttttgctcaa gctgtcaaag gatgggcaaa aatatcgatc aattaagaaa
    43441 taaagacaac tcgttatttc tacttcaata gaccctttta gaggttgtga catttttttg
    43501 tcacagcccc ttttttattt gaaaaatatt tctttctcaa aaaacaagca aggcgcttca
    43561 aagttcttct tgtatgcgct acacttagag taaagaaagg cgggaaatta actatgtcag
    43621 aaagaatcat ttatgcagat cgtccatttc gtgatgaatt tatcgaagaa atccatacta
    43681 tagcgcctgg ttatatcttt aaaacagaat tacaaccaga ggatctacaa aatgtagaga
    43741 tcagtttggg ctggaactca gactaccaag aaaaactatt agcctcttct accttgaaat
    43801 gggtccaatc tatctcagct ggtgttgatc agctacccct tgagactttt tcaaagaaac
    43861 atattttact ttcaaatggt agtggcatcc acagtgaatc gattacggag catgtcttag
    43921 gaatcatcct tgggtattct cgtggtcttt ttcaagcaca aaaggcacag agtgacatga
    43981 aatggttagg ttcatcggtc cattaccaat ctatccaagg gaaaaaaata ctaatcattg
    44041 gaactggcca tattggaaaa atgattgcaa aaaaatgcaa agcattagac atgaactgct
    44101 atggaatcaa tacatccggt catccagcag aaggaatgga aaagactttt tctttaaatc
    44161 aactaaagga agttcttcct gaagtagatt tcgtagtcaa tatcttacct ctaacggagg
    44221 aaacaactgg gctctttgat cagcatatgt ttgaacactt tgatccaaaa tctgtattca
    44281 tcaatgttgg ccgtggtgct tctgttaaga cacaagatct ggtgcaggct ttaaataacc
    44341 agcaactttc ctttgcagca ttagacgttt ttgaggaaga gcctcttcca caagatcatc
    44401 ctctttggaa aatggacaat gtactgatta cttcccacat tgcaggtttg acacctgatt
    44461 ttcaaaagaa attaatggct atttttttaa ccaatctgaa aagctacttt gccacccatg
    44521 agcttcaaac caatcaagta aaattgtctg caggatatta atagctgaaa acaatatttt
    44581 caaaagaaaa aagagaaggt gacaaaaatt gtgtcaccct ctcttttttt attgtttttt
    44641 aaagtgatag gttggctcct ccaggttggg attataatca acaagtaaat catatccttt
    44701 aaagaaccat tcatctgctt cctcgataaa aaaaagtgtc ccatcaattt ccactttctt
    44761 tacaggatgt tctggctgtt caactgacat tccaatcgaa aaaccatcat gaacctctgt
    44821 tttgccgtag acttttccaa aaaaacgtat tccgtaagtt tctggcagat caatttctga
    44881 gtcaaaccac ttttgtgctt ttggcgttac cgttaatttc atcccaacaa cctactttct
    44941 cttttctttc tattgattca tcttatcgtc tagaatatcc acatcgcctg tctctccaat
    45001 aaccagtaga ttatcatttt cacgaacata ttcatctgct gcaggagaag cgataaccgt
    45061 ttgatctact cttcgtatgg cgactactgt taagtcaaaa cgttgcctga aatttaattc
    45121 agctaatgtt ttgttgaaaa atttaggatt ggagactcgg atttctgcta aagaatattt
    45181 atctgacaac tctaggtaat ctaaaatatt ctttgacact aaattatgcg caatccttat
    45241 tcccatgtct cgctcgggat ggacgacgcg atcagctccg atcttttcaa gtactctggc
    45301 atgatactca ttttgggctt ttgctaaaat atttggtacg cccatttcct ttaccatcag
    45361 agtaactaag atactggctt gaatatcttc tccaatagcg acaattacat gatcgaaatt
    45421 tcttatgcct aatgatcgaa gtgtcatttc atcctgtgca tttgccacaa cagcatgagt
    45481 ggctatattc atatattcat tgacccgatc ttcattacta tcaatagcca gtacttcttg
    45541 accagcttcg atcagtgttc ggcagatact tccgccaaat cgtccaagac ctacaattgc
    45601 aaaattttgt ttcaccgttt tcctccttta gtatcttctt ttctcagtat aacaaagcct
    45661 ttacaaaaac aaaagcaagc atcattattg taaaaaagcc aagaaaaata atagttattt
    45721 tctcagcttt tgattatcct ctttatcatc gtttttattt caccaaacca tgtttgaatg
    45781 catagatagc ggcttgtgta cggtcttcca cttctagctt agccaatata ttggaaacat
    45841 gtgtttttac tgttttcagt gtaataaaca attcgtccgc tatttcctga ttactttttc
    45901 cttcagaaat cagcattaaa atttcatttt cccgattcgt tagttcttca tgtaatacag
    45961 gctcatttcg ccgactcatt ttttccatca tttttgttgt cacttctggt tctaatactc
    46021 gctcgccacg ttgcgttgcc cttatagcat cagcaatttc atgtgcggta gaggtcttca
    46081 acaaataccc tgctgcacct gcttcaatgg caggataaac tttttcgtca tcaataaaac
    46141 tggttacgat aatgatttta gcttctggcc aatcttttaa gattgctttt gttgattcga
    46201 taccatccat ttcttccatg actaagtcca ttaaaatgac gtccggtcga agtgtcatcg
    46261 ccttctcata gccttgtcgt ccattttcag cttctccaat tacttcaatg tcttcttgaa
    46321 tagataaata aaaagagacg cctaagcgca ccatttcatg gtcatctact aataaaactt
    46381 ttatcatcct tatctatgcc tccttcatca aagggacctt gatttctaca cttgtacctt
    46441 gacctttaaa actaatgatt tttactgttc caccaatacc agctactcgt tctttgatat
    46501 tgttcaatcc ataacttccc gttttcgttt cattcatatt aaaccctgtt ccgtcgtcaa
    46561 taatacgtaa caaaagatta ttgtctattt tgtgcaaata tacctctaat tcattggctt
    46621 tagcatgtct taatgtattt gataacaatt cttgaacgat tcggaacaag tgatcctcaa
    46681 tggaactaga tagttttaca tcttctacat cccatttcag cgaaatctgg attttatttt
    46741 gcaattcctt caataactgc tcgatacctt gctttaaact tttctcttct aaattgactg
    46801 gacgtagatg aagaagcagc gcacgcatct cagactggga agcgttgatg atctctgcta
    46861 ccattttcaa ctgcttgcga aacatttctg gtgtctcgct tttttctgct tgctccgtca
    46921 atgccgacat catcatcatt gctgcaaaca attgttgcga aactgaatca tgcagctcac
    46981 gggctaatcg atgtctttct aattctaaaa tttcttcttt ggtttgtcca tctacatatt
    47041 ggggacggct cgttacaatt tgtaattcac tagatacttc aatcattttt tcttttatct
    47101 tagtgatttc cttatcaata tctttgatgt ataaatcgtc cgatgcactt gggatcggaa
    47161 gaagcaacaa tttggattca taattgccag aagaaagtgc acgcagcttt tcttcgattt
    47221 tcccgtattt tgtcttttga ataagtgata ataatagaaa gacaattaat cctacaccta
    47281 aggaaatggc taacaaatgg aagataagcg gaacatataa taaacgtgca cgcagtaatt
    47341 ctccccacca atgactttga ttgctggcat aaaaataagt gaaaagtgaa aatagaataa
    47401 taaggaaagc cgcaatcccc gaataaacag ctagcatcgc tctggatatt tttcccatca
    47461 tattcgtatc acctcgacat ctccaatcaa ggtattagta ataatcttca atctacgagg
    47521 gttttcatca taatcactgc tgtagatttt gatttgttca tttttcaaag aaaattgctc
    47581 ttcttcaaac aagacatttc ctactaatgt cgcatgctct aatcggatgg ctacgcccaa
    47641 aggtactaag atccgtgttc tgcctatccc ttttcttaca atcacaatat tatcatcttt
    47701 tggcaaaagc gtattgccca aatctatgat agtatcacca gaaatgacgg cgatgttgat
    47761 atcgtcccat tcatagacgt cgtttccaat acgttggtta ccgaataatt gctgtctttt
    47821 ccgctcatta ttatgtgttt ttaattgctc tgtttggatc atcatgatct gtttctttcg
    47881 ccaaaaagca tttttggtta aatctatccc tgaaatctct acacctttca gaccaataaa
    47941 taaaacggca aatacagcca tcatccataa agcaggactg ttgactaagc taaaaaaaat
    48001 gaccaaactt cctaatatca attgaaaatt ttgaaattta gtacgaggat gtttgcgcat
    48061 agctagatat atattgaaaa taccgaatat cactaaaagc aaaagtccag tattgtttac
    48121 gatttgccaa acagcaaata acagcagtag tgcttcaact accacaaaaa aacgccaaga
    48181 actattcatc gctctttctc cctcctgaca cattctcatt tcatcatacc tattttttta
    48241 actgttggct ataggacttt agatgtaaga tcgtttgttg aaatctaaaa tacacaaaga
    48301 gaaaaagatc tagagtgtat catcagtaag atgaactctc tagatctttc agtcagacta
    48361 cgcttgttct actttgacga ttttaactaa catatcgcca cctggagtag caatagttac
    48421 ttcatcattt ttacgtttgc caatcaatgc ttgcgcaata ggagaatcat tggaaatttt
    48481 gccagaaaac ggatctgctt cagcgcttcc gacaatcgtg tactcttctt cttctccatc
    48541 tggtagttcg atgaatgtaa ctgttttacc aattgatacc tcatctgcat ctacaccgtc
    48601 gttatcgatg atttgagcaa aacgaatcat attttcaatt gttgtgattc ttccttcaac
    48661 aaatgcttgt tcatctttcg ctgattcata ttcagagttt tcggaaagat ctccgaaacc
    48721 gcgggcaatt ttgattcgtt caatgatttc tccgcgtttg actgttttta gttcttctaa
    48781 ttctttttct aatttttctt taccttctag agtcattgga taaactttct ctaccatgtg
    48841 taacgtctcc ttttttcgat aaaataccgc aaaaggtaat cttttgagga atatagtcca
    48901 aaaaagatta ccacgctttt cttcaaatgt aaatgttttt ctaaaaatga tcaatgtcta
    48961 gagcctgttt ttctaagaat tatttacata tttttccact aattccatat gttcttcata
    49021 agttttagcg aaataaacat ttccagtgct cgtatctgct acgaaataat aatagtcatt
    49081 ttctgctggt tctaatactg ccttgattgc ctcttcgctt ggattgtcaa aaggtccagg
    49141 tccatagccg gtattggtat acaaattata tggtgaatct acagctgtat cttcatacgt
    49201 cactaattct ttgtgttcac ctaatgcgta taaaacagag atatctgact gaagtggcat
    49261 atttgctttg attcggttaa agaagacttg tgcaatgttt ttacgatcat tttcttttac
    49321 gccttctttt tcaactagag aagctagagt taaaacttct tgtacagtca aatttttctg
    49381 cttgatagct gaataatagt tttgcatgac cgtattcgtc ttgttcacca tttcaatcac
    49441 tagatcctta agcgaagtac tcgtgtaata atcatatgtg gctgggaata agtaaccttc
    49501 caaacggtat tttgtgttct cagctttaga tgcactttcc aacagttctg ggaatttttg
    49561 gtgcaactcg ttaaagaaag tctcatcatt cactaaatct aaaaattctt tcttgtcttt
    49621 accagttacc ttcgctaccc gttcagcaat ctgatcgata tcgtaccctt ctggaatggc
    49681 aattttccca tctgcaactt ttgttggctc agaagtaccg ccttcttgca actgtttgcc
    49741 aatctcgtca agagtcatat taggcgctaa ctgataatag ccagcttgaa agccagttaa
    49801 atttttaaat tttgtataat aattgaaaac aatcccgctt ttaataatat tgtccttctc
    49861 caaaatctca ccaatctgct tgttagaaga gccactcggg atctcgactt ggactaattg
    49921 gtcatctgat ttatctaaag gcttcaaccc gctatctaca taacgataca cggtaaatcc
    49981 aagaaatccg ccaataatca ataatgtaag agcaatcact aaaatgatct tcctcacaat
    50041 cttatcttct tttttacgca gatttctccg cttgtcgatt atatctgctg tagctgcctt
    50101 actggcttct tttttatctt tacttgggtg ttgtttgttt cctgacaaaa caactcctcc
    50161 ttactatcaa gttcgtaact ttattcatta tacatgatat ttgaattggc gtgaatgatt
    50221 ttatttaaat attctatcaa aaatgtaagt agtaaatcca ttactataag attaaaataa
    50281 atgttatcaa gatacaagct attttagctt tattctttct gtaaaaaaga aaggagacta
    50341 aataaactat tatgaaaaaa ggaaaacatg ctaaaaaaag aaaatattca tttttaatga
    50401 tcgttgtcgt gttgtttagc cttagtgcgt tctctacagg aactattctt ggatggcaat
    50461 tttacaataa tgaacactct cagatagttg cccatgctca atcagctcaa aaaaagttga
    50521 tactgactac caatgaacaa cagctcgttg aaaaaatcga tcaactatta agaaaaaatg
    50581 attatattgg ctcaatttac gtaaagaaaa ataatcgcat catcatggaa aaaggctatg
    50641 gctatgcgaa taaactcacg caacaaccaa atgatccttc tctttattat cagattggtt
    50701 cggttcaaaa agcattgact actcttttga ttttaaaaca agtcgagcgt gggcaccttt
    50761 ctttagatac taagctggct caattctatc cacaaattgc tggcagccag caaattacta
    50821 tccgtgactt gctgtatatg cgctcaggct tgcaccgaac tgcttctcca accataccga
    50881 tgactgatga agaagtcgtt caatttgcga ttcagcactt aaaatttaca gattatcaga
    50941 cctctcatta tgattcattg aactttacca tattgactgg aattttgatc aaattaacaa
    51001 atcaaccgta tgaaaaatta ttgaccgatg atatcatcga acctttagga ttacagcata
    51061 caaaatttta tgaccaagtc aaaaattcat ccaatcatgc actatcctat caaatgtcta
    51121 aagaggatga ttactttaaa ccactgacag aatctgaaac agagatccat aacgaattag
    51181 ggactggtaa tatcagtatg tccgtctatg atttaaatac gctgtttaca caagttttat
    51241 ctggaaaact agtttccaaa gacttactct tctcattatg gcgtgaaaat gcgaatggac
    51301 atccatatag tggtggggta tatagcggag ataattatat cctgtcgcaa ggaaatatcg
    51361 actcttttga tacatctgct atatttagca aagattcaca ggatgctatc atcatggaaa
    51421 gcaatataaa agctgacaaa actatcaaac tgcctactgc tgatctaaga aaccaaatct
    51481 atgacctgat ggaaggaact actaagttgg caaatagctg atatgatgaa aagatagcaa
    51541 atactacttc tttgaatagc atttgcttaa aaaaagtact cgactaaacg taggaaagag
    51601 tgtgggacaa atctaaaaat caatttttgc tacagacttt aattccaaat aaacagaaca
    51661 gaaagaggtg ttcttctttc tgttctgtcc aaattattct aagatttctc tttaccaaca
    51721 ttatttgaca aagggcctat taaatctaaa atagtatctt tagattgcta aaatatgtgc
    51781 tagcaaatca ttcatggctt gttcttattt ttaattttat gcaataaaaa agaagaacaa
    51841 ttagcgtgat tatagaaata aataatgaga ttttagaaaa aaatgtgtat gaatacctga
    51901 ttgttacttt atttattcca ctttcacttt ttactgtaac aaaaggacct ccttgtttta
    51961 gtactgtagc attttttgaa ttaacagtaa ccgttaatcc tttataccgg aatacaggta
    52021 aaactattgt tttgcctttt gaagatttaa acgtaaatgt aatttcatct ttatttggag
    52081 aaaagctact ataaataact tgaccatctt ctgtaacttg atggtttttc aaaagcaatt
    52141 gatctttttt atagcctata ggtacataat caaaattctc aaattcttca agtttatttt
    52201 ccatttgttc attagaataa gttccttgaa aattcatttc tttcaccatg ttaactactg
    52261 atgaataatt taaaattgaa atagctaata ttaataatag tataaaattt cttctccttg
    52321 tataagaaaa tttttccaac tgtggagaaa taaatagaga tactgaaaaa gatatgaaca
    52381 atgttgaaaa tatcataaaa c
//
LOCUS       DAYYFD010000006        51499 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000006 DAYYFD010000000
VERSION     DAYYFD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.006"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHA25_000338"
     CDS             <1..1412
                     /locus_tag="ACHA25_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8761674.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="ACHA25_000339"
     CDS             1546..2889
                     /locus_tag="ACHA25_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8761675.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3001..4452
                     /locus_tag="ACHA25_000340"
     CDS             3001..4452
                     /locus_tag="ACHA25_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8761676.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4801..5433
                     /locus_tag="ACHA25_000341"
     CDS             4801..5433
                     /locus_tag="ACHA25_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8761677.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            6021..7460
                     /locus_tag="ACHA25_000342"
     CDS             6021..7460
                     /locus_tag="ACHA25_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8761678.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7755..7973
                     /locus_tag="ACHA25_000343"
                     /pseudo
     CDS             7755..7973
                     /locus_tag="ACHA25_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            8222..8437
                     /locus_tag="ACHA25_000344"
     CDS             8222..8437
                     /locus_tag="ACHA25_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761679.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8654..8910)
                     /locus_tag="ACHA25_000345"
                     /pseudo
     CDS             complement(8654..8910)
                     /locus_tag="ACHA25_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9097..10008)
                     /locus_tag="ACHA25_000346"
     CDS             complement(9097..10008)
                     /locus_tag="ACHA25_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8761680.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(10027..11031)
                     /locus_tag="ACHA25_000347"
     CDS             complement(10027..11031)
                     /locus_tag="ACHA25_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8761681.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(11028..13151)
                     /locus_tag="ACHA25_000348"
     CDS             complement(11028..13151)
                     /locus_tag="ACHA25_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8761682.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13156..15603)
                     /locus_tag="ACHA25_000349"
     CDS             complement(13156..15603)
                     /locus_tag="ACHA25_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8761683.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15590..15979)
                     /locus_tag="ACHA25_000350"
     CDS             complement(15590..15979)
                     /locus_tag="ACHA25_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8761684.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(16039..16542)
                     /locus_tag="ACHA25_000351"
     CDS             complement(16039..16542)
                     /locus_tag="ACHA25_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8761685.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16555..16779)
                     /locus_tag="ACHA25_000352"
     CDS             complement(16555..16779)
                     /locus_tag="ACHA25_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761686.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16883..18793)
                     /gene="ltrA"
                     /locus_tag="ACHA25_000353"
     CDS             complement(16883..18793)
                     /gene="ltrA"
                     /locus_tag="ACHA25_000353"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8761687.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(19539..19676)
                     /locus_tag="ACHA25_000354"
     CDS             complement(19539..19676)
                     /locus_tag="ACHA25_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8761688.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(19673..20857)
                     /gene="mobT"
                     /locus_tag="ACHA25_000355"
     CDS             complement(19673..20857)
                     /gene="mobT"
                     /locus_tag="ACHA25_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8761689.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(21116..21406)
                     /locus_tag="ACHA25_000356"
     CDS             complement(21116..21406)
                     /locus_tag="ACHA25_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761690.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(21396..22538)
                     /gene="dcm"
                     /locus_tag="ACHA25_000357"
                     /pseudo
     CDS             complement(21396..22538)
                     /gene="dcm"
                     /locus_tag="ACHA25_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(22614..22778)
                     /locus_tag="ACHA25_000358"
     CDS             complement(22614..22778)
                     /locus_tag="ACHA25_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761691.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(22771..23445)
                     /locus_tag="ACHA25_000359"
     CDS             complement(22771..23445)
                     /locus_tag="ACHA25_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8761692.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(23523..24863)
                     /locus_tag="ACHA25_000360"
     CDS             complement(23523..24863)
                     /locus_tag="ACHA25_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8761693.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(24946..25281)
                     /locus_tag="ACHA25_000361"
     CDS             complement(24946..25281)
                     /locus_tag="ACHA25_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8761694.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(25291..25665)
                     /locus_tag="ACHA25_000362"
     CDS             complement(25291..25665)
                     /locus_tag="ACHA25_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8761695.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(25678..25992)
                     /locus_tag="ACHA25_000363"
     CDS             complement(25678..25992)
                     /locus_tag="ACHA25_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8761696.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(26105..29332)
                     /gene="fss3"
                     /locus_tag="ACHA25_000364"
     CDS             complement(26105..29332)
                     /gene="fss3"
                     /locus_tag="ACHA25_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8761697.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            30189..30443
                     /locus_tag="ACHA25_000365"
     CDS             30189..30443
                     /locus_tag="ACHA25_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761698.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            30607..31524
                     /locus_tag="ACHA25_000366"
     CDS             30607..31524
                     /locus_tag="ACHA25_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8761699.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            31517..32299
                     /locus_tag="ACHA25_000367"
     CDS             31517..32299
                     /locus_tag="ACHA25_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8761700.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            32300..32980
                     /locus_tag="ACHA25_000368"
     CDS             32300..32980
                     /locus_tag="ACHA25_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8761701.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            32973..33869
                     /locus_tag="ACHA25_000369"
     CDS             32973..33869
                     /locus_tag="ACHA25_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8761702.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            34081..36003
                     /locus_tag="ACHA25_000370"
     CDS             34081..36003
                     /locus_tag="ACHA25_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8761703.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            36004..36450
                     /locus_tag="ACHA25_000371"
     CDS             36004..36450
                     /locus_tag="ACHA25_000371"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8761704.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            36466..36780
                     /locus_tag="ACHA25_000372"
     CDS             36466..36780
                     /locus_tag="ACHA25_000372"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8761705.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            36806..37855
                     /locus_tag="ACHA25_000373"
     CDS             36806..37855
                     /locus_tag="ACHA25_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8761706.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            37867..40470
                     /locus_tag="ACHA25_000374"
     CDS             37867..40470
                     /locus_tag="ACHA25_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8761707.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            40587..42236
                     /locus_tag="ACHA25_000375"
     CDS             40587..42236
                     /locus_tag="ACHA25_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8761708.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            42552..43487
                     /locus_tag="ACHA25_000376"
     CDS             42552..43487
                     /locus_tag="ACHA25_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8761709.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            43480..44250
                     /locus_tag="ACHA25_000377"
     CDS             43480..44250
                     /locus_tag="ACHA25_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8761710.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            44665..45648
                     /locus_tag="ACHA25_000378"
     CDS             44665..45648
                     /locus_tag="ACHA25_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8761711.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            45969..47006
                     /locus_tag="ACHA25_000379"
     CDS             45969..47006
                     /locus_tag="ACHA25_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8761712.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(47095..47418)
                     /locus_tag="ACHA25_000380"
     CDS             complement(47095..47418)
                     /locus_tag="ACHA25_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8761713.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(47405..48112)
                     /locus_tag="ACHA25_000381"
     CDS             complement(47405..48112)
                     /locus_tag="ACHA25_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8761714.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            complement(48411..49835)
                     /locus_tag="ACHA25_000382"
     CDS             complement(48411..49835)
                     /locus_tag="ACHA25_000382"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8761715.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            50010..50723
                     /locus_tag="ACHA25_000383"
     CDS             50010..50723
                     /locus_tag="ACHA25_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8761716.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            50872..>51499
                     /locus_tag="ACHA25_000384"
     CDS             50872..>51499
                     /locus_tag="ACHA25_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8761717.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFAL"
BASE COUNT        16152 a         9571 c         9715 g        16061 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
     3061 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
     3121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
     3181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
     3241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
     3301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
     3361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
     3421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
     3481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
     3541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
     3601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
     3661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
     3721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
     3781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
     3841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
     3901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
     3961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     4021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     4081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     4141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     4201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     4261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     4321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     4381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     4441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     4501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     4561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     4621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     4681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     4741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     4801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     4861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     4921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     4981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     5041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     5101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     5161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     5221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     5281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     5341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     5401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     5461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     5521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     5581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     5641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     5701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     5761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     5821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     5881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     5941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     6001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     6061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     6121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     6181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     6241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     6301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     6361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     6421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     6481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     6541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     6601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     6661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     6721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     6781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     6841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     6901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     6961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     7021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     7081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     7141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     7201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     7261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     7321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     7381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     7441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     7501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     7561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     7621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     7681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     7741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     7801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     7861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     7921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     7981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     8041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     8101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     8161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     8221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     8281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     8341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     8401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     8461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     8521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     8581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     8641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     8701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     8761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     8821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     8881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     8941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     9001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     9061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     9121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     9181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     9241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     9301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     9361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     9421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     9481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     9541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     9601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     9661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     9721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     9781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     9841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     9901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     9961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
    10021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
    10081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
    10141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
    10201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
    10261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
    10321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
    10381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
    10441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
    10501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
    10561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
    10621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
    10681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
    10741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
    10801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
    10861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
    10921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
    10981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
    11041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
    11101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
    11161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
    11221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
    11281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
    11341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
    11401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
    11461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
    11521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
    11581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
    11641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
    11701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
    11761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
    11821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
    11881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
    11941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
    12001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
    12061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
    12121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
    12181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
    12241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
    12301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
    12361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
    12421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
    12481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
    12541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
    12601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
    12661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
    12721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
    12781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
    12841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
    12901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
    12961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    13021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    13081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    13141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    13201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    13261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    13321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    13381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    13441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    13501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    13561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    13621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    13681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    13741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    13801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    13861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    13921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    13981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    14041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    14101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    14161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    14221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    14281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    14341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    14401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    14461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    14521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    14581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    14641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    14701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    14761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    14821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    14881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    14941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    15001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    15061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    15121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    15181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    15241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    15301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    15361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    15421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    15481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    15541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    15601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    15661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    15721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    15781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    15841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    15901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    15961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    16021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    16081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    16141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    16201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    16261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    16321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    16381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    16441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    16501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    16561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    16621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    16681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    16741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    16801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    16861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    16921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    16981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    17041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    17101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    17161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    17221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    17281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    17341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    17401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    17461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    17521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    17581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    17641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    17701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    17761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    17821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    17881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    17941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    18001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    18061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    18121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    18181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    18241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    18301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    18361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    18421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    18481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    18541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    18601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    18661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    18721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    18781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    18841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    18901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    18961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    19021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    19081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    19141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    19201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    19261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    19321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    19381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    19441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt ttcactaagg
    19501 aacgagtcgc acataaattt tgtccgaaat tcatcttgtc actttccttt cttttccatt
    19561 gctcgatccg ctgctgcact tgcattcatc aagcacatca cagtaacacc aagcatactg
    19621 ccaccaaata ccaaaaaaat atcttttaca attcccatga cgattcctct tttcatataa
    19681 tcaaattttc aattgccata ctttgttgct ccaaaatctt tttatgtcgg tctgataagc
    19741 gtgcattctg taccatatcc tggatatagg ttgtccgatt caatcgatcc aactccttag
    19801 ccattttcca cgtaggggcg acttgtcggc ctagccaatt caaggttcgt tcaatggtat
    19861 acggttttgg ctcggaagtt aattgaatct ctttccgatt ccgtccaata aaacgccccc
    19921 attgttcatt caagggccag ttggttcgcc ttttaccttc tactttatcc gcaaatcgca
    19981 agtaacgatt gataatggaa aacgtggtgc tctcaatgct acgacctttt aacaaatctt
    20041 gaatcgcatg aaaagcacgg tcatttttca gtcgaatctc aaaacgattt ttcgtctcag
    20101 tttggtctaa tggaatccct aacttgatat attgttcata gtctttttca tacaaacaga
    20161 aataaacctc actcttgaga ctgccgatat ataaggtatt gcccatgccg tccttttcat
    20221 cagcctttaa aagttccccg gaacgataac ttttaaaggt acgaaacaag gagatacatt
    20281 cttccttctg acattttttc gttagctcag gtatatccaa tagccccact cgatcattga
    20341 ttgccaagtc caagcgtttc atcacgccac cggcctttag acaatcctcg aaaaagtcgt
    20401 accagctacg cttttgagcg agtaaaaaag tttcaaattg tcggcaacct cgccccttta
    20461 attctaaaag cacaccttta tcttcttcat gagacaacat caccacaata tcacccatga
    20521 catactgttc ttgataggag taaaaggcat aatcttcatg gagcatatac tcaaatttca
    20581 aatggagaat ttcttgaata atttttcgtg catccgttgt gggaaagcga atccttacat
    20641 agtcaaacag taacgtcaaa ggcaaatcgg gattaaagct ttccagttgg tcaaaaattt
    20701 tcctcatggt ttttatagat gcggttacgc caccattttc tatctgattt aagtacggcc
    20761 gactaagtcc acaagccgta gccaatttat tttgtgaaac gccgtattcg ttgcgtcggt
    20821 cttttaatag ccgataatct aaattccgtt gtgccaagtt ctcacctcca aattcaaggt
    20881 gttaactttt tcaaatgagt taacacctat acaaattact tactatcttt gatacaacaa
    20941 gcattcttaa tggttgctta cttatttttt gtgtttttgt acccccctgt tagatacggg
    21001 ggtgagacat catggtcggc taacgccgtc catgaacagg cacagcacca cggaggattt
    21061 ctgcttcgcg aatcctccgc ggtcagcgcc taacagtcag ttgtgttggt ttaggttact
    21121 catctgtaat accatttact tccaactctg ctttcatttc taacaagtat tgacgaatat
    21181 ctggttcttt tgcttggtcc gcaatccgct caaattctgc aattacttga tttatttcaa
    21241 ttcgagcatc cgtctcctca ttcattaaat atggatcgcc agcgactttg acaccattct
    21301 tgcgaatatc aaattttaac cgtcgcaatt gttcttctaa ttctgggtct gactgaatct
    21361 ttccaatcac ccaataaatt tctccaatca actcatcata aaccatgtac atccaactct
    21421 tttaatcttc tggcaatatc aaaaatgacc ggaactgtta cagagtttcc ggcttgctta
    21481 tacaactgac tatctgaatt gacttgagaa tctcgatcaa aagcccaatc gggaaagccc
    21541 tgaagtcgcc aacactctct aggtgttagc ttccgaattt gatactgatc cgtgacgatc
    21601 gcttgttggt ctcctgttag taaggtatga acactctgtt ttcccagacg acctcggcga
    21661 cttgtggaat taggaaaagc cagattcaca ctgtcttctg gcttcacctc ttgatattct
    21721 gtttcatttt ttcgaatcat gatttttgta ccaccttgtt tcgttgacaa ggtgggggcg
    21781 gttccagtaa cttcataaac tcgattacct tgttcaaact ttcccggtaa tagtcctgct
    21841 acagagattc catcccttgc tggcagtgcg attttgattc cttcatcacc tgttaaggtt
    21901 ggcgctacgc catctgaacc atagacttgt ccacccattc ctcgattgga aggattgata
    21961 tttaccaaat ttctgatgtt ttgatcagat gttgaagacg gtccaatgat aggaaatact
    22021 tgttcggtac atgcgtctcg agaatgtccg ataaggaata tccgctcccg gctttgggga
    22081 atgtaggcct ttgagttaag cacctaccac tccacgtcat accccaatcc atccagcgtt
    22141 cggaggatcg tctcgaacgt agcccctcct tcgtgattga gcaatcctcg gacgttctca
    22201 aggaataaaa gcttaggtcg gagaagagtg gtgaaccgag cgatttcaaa gaataaagta
    22261 cctcgagtat cggaaaatct ttgccgcttt cccgcaattg aaaaagcttg gcacggaaat
    22321 ccgccgcaaa ggatgtccac tggtccgagg gattgaataa actcatctga tacacttgtg
    22381 atgtcatgca tttccacctc cttacttgta tcatgaattg ctttataact acgtctagca
    22441 aatttatcaa tttcacaaaa accgatacaa tgatggctcg cctgttccat acctaaacga
    22501 aagccaccaa taccggcgaa taaatctaaa aatctcatac attacctcat tcattgcaaa
    22561 gaaaaaagcc aagaacattt tgctcttggc tcatacatta ttaatttgtc ctactatttt
    22621 ttatttaatc caactaattt ccgttgtttt tcattggcct gatcggctac atatttaatc
    22681 accgttttga actcttttgc agtagcattt tccgataaat ccgcaatgct cttattgtat
    22741 ttctttacaa ttttcgttac ttgttctttc tcagtcatgt aattgaacct ccaaatcttt
    22801 cctactatac cctagtggta aaaataattc tagattatcc gtagcataat gaacttcgga
    22861 aatattattc gtataatctt ttattatgat atgaagcaat acatctggat gtttactctt
    22921 tacttttgca atgttctctc ttgatttgaa aaaagcgtta ataaatgcct ttttcggcac
    22981 atgacgtccc tctgcaattt cacgttcttg tgtaaatttc caagcgattt tagggtcttg
    23041 ataaacatag aaaatcgtca ctcgaaaatg ttttttcaac gcccgcgaga tatttttttc
    23101 agcacttaat attgcaaaag ttgcatccag aataaaagaa tagccatgat ctaagacata
    23161 tctaaatgcc tgctctacca accaagaaga tgctttttga aagagactag agttttttcc
    23221 attataatca ggaaactggg tgcgaaagta atccgcatca ataatcacat ggttatcata
    23281 attctcagct aatccaatgg caacctctga ttttccagcc ccaggactcc ctgccataaa
    23341 aactgcgtct cgtccaactt cgggggattt ccctttgata atataagaaa taaacttatc
    23401 cttatttgct tttgcatact ctaaataaga atcattactt tccatattaa ttatcccctt
    23461 tcttcctact atataaaagt attatacgtt acttgaagta taaagtcagt attgaagatc
    23521 actcaatcat tcatcttttc taatttacta attgaatcta agaaatcata cccttttggt
    23581 acaagtggcg tataaaactc actaatcaca ctgccacctg tatccacata acctcgacct
    23641 ttgatgtgct tcataaagaa gtttttttcg acctccccaa acatcatgga atatccgagt
    23701 tcactcatac gacccaaagc cacgcgaaag ttgaattgat cgcggattcc gtctcctaaa
    23761 tatttcgcat ccggcctttg gcaagccaaa atcagaaaga aaccagattg acgacccaac
    23821 atcacgattt gtttgagctt attcaaaatc accgcacttt ctttcgtcgt taacatctcc
    23881 atataagcca cgtattcatc aaagattaaa aagttgggtg gaagtccaag ataggcataa
    23941 ttctctcctg ttttgtagtt agacatttct ttcattgcct tgctacgagc catcatacgt
    24001 tcatagaaat cttccacaca agcagaaatt tcttcctttt gtgaataaac atgaggcatc
    24061 accgtaccta aatcagctaa atctgcattt ttgggatcga ggataaacaa ttctgcatct
    24121 gacttcaata gtgcttcgat aatggtgagg aggaaatagg tcttccctcc acccgtgcca
    24181 ccagcgatta acatatgggg taaagaatca taagcccata cttgattttt catcaatcgc
    24241 agagtcccat tttctgccac tacttcgtct atcccaattc gattggcgat catatcatac
    24301 agtaaagtat actccacata ggagtctttg agttcctttt ctactaactc acaatacagt
    24361 ccgctttcta acttcttttc caattttaaa agctgatctt ggtatttccc aagtgaaatt
    24421 tgaacgcgaa tagacagtaa tccatctttc attcgataac agattttggg gaagtaactg
    24481 atagtctctc tggttcggct actattcaaa tctttgaaaa agccttcact ttgagtttgt
    24541 ttcacttcat accaatggtt ctctagaatc atccgagcta gcttttgtcg atgccacaag
    24601 cttcgccaat gatcaagaaa aaagtgaata taaagcaata tcatagctcc tgaaaaagcg
    24661 agacttccaa gcagacatac taaatctttt atagaccaaa cgatttcaat agtagaaagt
    24721 gatagcttag aaatatcaaa ttgtctaaac tcttgaaaat aaaagacgct catccaaccg
    24781 atgaacagag cgagtagcca gccaagaaca aagtgataaa ccaagtgctg atctcctgcg
    24841 cgtatgcgat gtcctttgta cattttgatg gtttccacct ccaatcgaaa aagcccaata
    24901 ggatttcatt aggcaatttc aaaaagttat gctagtcatt tactgctacg aggaggggag
    24961 ttgtttcgtt tcaaccaatt gatttaaaaa gccttcaata gcatccggca tctccacacc
    25021 aatttcttcc aaaatggatt ctgcctgccg acataagttt tcatttactt cgacaactat
    25081 aaagaaagac tcttcttctc ctaaccagtc tagtacatca gagtcttctt cactgtcgct
    25141 attttctcca agtaaacctt gatgtataat cagaaacatt ttttcatcag aggttaatgc
    25201 tttaacgagt tgttcctcag tcagctcgcc ttttccatat tgatcgagac aatctaaaat
    25261 atgttcaaca ttaatttcca taatcttcac ctatttcttt ctttcatttt ggttcgtagg
    25321 atttccttga ttcggctgcc gcttcaagac taaatcatcc gccttaatga accaattaac
    25381 ggtagttcct tctcgaaaaa cataattggc aacggtatcc acgattggat tgaccaactc
    25441 tacctctgca tcataagcaa actcgcgtaa gggtacatta gctgggatac taacttgaat
    25501 cattcgccct tgtgcagagg attttaaatt ataggttcgt tcttttacct cattaccaat
    25561 tgttccatcc tcattttgct tccgtacttc tcggcgtaat gcggaaaaac gcaacttccc
    25621 aaatgttaaa cctgaatcta cgacaatacc ttctgttaat ctcatcttct tttcctccta
    25681 tgctttcact aaatcatcaa catacaacac gtaatcagta aacccacgat tctcaatctc
    25741 atagcctttg gcaaccaaat ggggattcac ggcttttagc ggttggtctt gatcaaaatc
    25801 cttttcaccg gcttccgcag ctactaccac ctcaatatca tcggcccgtt gcttactgga
    25861 atacaaatga taactgcgac caatcaccgt agtattccgt ccatatcctt ctgttataat
    25921 ttctccttca ccggcataac ttaactttcc aaaggtttca gccatatctg gcacgacaaa
    25981 tttcaattcc atcttctttt cctcttttca ttgttagttt tggatacaaa aatagcgcga
    26041 ctactttttt aggaggcggt cgcgcacgtt ggttcttagg atttacttgt atcatctcat
    26101 caccttagtt ccggcgattc cagaaataat agcccgccgt cgcaaaaatc aaagtaaaac
    26161 caatgaacag taaaacattg gaatttttct ccccggtttg agggaaggtc ttcactgtgg
    26221 gactagattc ttgagagcca cttggaacag ttggtgtttc cggttgtttc ggcgtactta
    26281 gtatggttgg aacttctttt ggtgttaagg tttgagattt ttctttcaaa tcttcattgt
    26341 gttttccatt gatattccca tctttatcgt aattgatttc cgcaaaagta aacttggttc
    26401 cttctggata ttttccggta tctacttttt tcgcaagcac agttttggtg aattctttat
    26461 catttacttc ataatcaatc ttgccagatt tccaaatttc tttgtttgta ccatccggta
    26521 ataaagcata cagaattgtt tcgaaagctt cttttgagcc atccagtaca tcatgagtaa
    26581 tcgacacatc atcaaacata tccaccacat caccatgagt aaaggtctga gaaccgtttt
    26641 ctaggtgggc ctttgtttgg atagaaacat ggtgttccac tgtacaactg acagtttggg
    26701 cttggttatt caagctaaca tccttcgcta ctggcttatc gcctgcttca aaagcttcct
    26761 tattttcata cacatagttg aataagacaa tagttttatt taaaacttgt tcggcggtta
    26821 atttatgctg aattttccaa gttcctgttt tctctttgtt cgccaacgtt gcgcttgttt
    26881 ctgcaatcac cactggtttg gctttttcat ctttttcttg ggcagctttt gtcgcatcca
    26941 catcaatggc ttgcgctacc acataccagt cttctttgat ttcatgtaga ttgtaactca
    27001 atttatcaac tagctcggta gaatccagag aagttaattc tttgtttccg tctttccaag
    27061 tcgcaagaga agttaaacta tcttcttcct caggcaaatc atagagcatt aaacggttca
    27121 aactaacaga aaatgctttg ttcgtcagct tctcgtaagg aacggtcacc gttttaattg
    27181 gttgtttttg gtcttgttcg gtaatggtaa agacatactc actcttcaca aagtcttctt
    27241 tgttttcctt aaaagtagaa cggatttcta atggcgtaat cttttgaaaa ccttctgggg
    27301 cttctacttc ttcaagtaaa taatccccat aaggaagatt ttcaaacttg ccataaccat
    27361 caaaccctaa ttgctcgtta taagctgtgg tcgcttcatc ttcggcacct gtgatttcat
    27421 tggtcccttc caatggcgac actttaaagg tcagatcatt gaagccagtt tcggtagtgc
    27481 ccgcagctga tccagcaaat ttaaagaaat caaagccaaa gcgaatcact tgttcttttg
    27541 ccgtaacatt tcgggtaatc acggcatttt tttcgctatc atccaccttt ttgatggata
    27601 caggatactt tgtttcatca aggctatatc cttcgggcgc ttttgtttct tgccaataat
    27661 actcgttgat agctaaatgt ttaacggcaa cttggttctt ttcgtctaaa gtcaaagtga
    27721 ccgtttcatc agaacctttc gttcccttcg ctaactctgg tttaaaagcc tcactccatt
    27781 taacagcttt tccatcctta gcagtaaaca aggtgtattc agcaccctca aagactgcct
    27841 taccttgtgc tttatcccca gtatctttgt cttctttcgt caaagtggtt tcgccagtga
    27901 cttcttgatt ttgccctttt acgttactgg taacaggggc tacggtttga ttggcatact
    27961 tcaattcgac ttttacaggt ttgaacgtat tcacgaaccc atgactagct ttagtttcgg
    28021 tcacataata ggttcccaat tccaacgcat tagcaatctc ttttggtgtt tccgcatgac
    28081 cgttttcatc cgtggtcatt tcttgaacga tttcaccggt tggactgtct ttgcgaatgg
    28141 caaacgtatt accagctaag gaataattgt cattccaaag atcactccct gtttcaactc
    28201 cgcttttatc cagaatgatt tgcccttttt cccgtgcgtt ttttgaagtc acgtaaatgg
    28261 tttcgcctgc tttaatggtg gtagtcatgg gggtggtatc aatcgtataa ggcgccggta
    28321 ctgatttttc ggtaatggtt acttttgtac catgaggaat ctcatccaat gtagcaatgc
    28381 cttctttgtc agtagtcacg tcttttgcag gtaaagtttt tccaaagtct aaatggaaaa
    28441 ccgttcctgg tacaacatca cccgattctt tatcgacttt tttgatcttt aaagaaccct
    28501 ccgtttccac atcaattttg attgtgtagg tattaggttt atcaatcgcc ccggccatta
    28561 aagtctgttg gccagccttt ttataggcga caggtgttcc ggtaccagca gatttcttaa
    28621 gcgtcagcac accactttct tttgaattgg catttggggt gattatcaac tgattaccat
    28681 tcacacgata atcaatgttt gcggcatttt ccacaacctc atcgaactct gacaaattca
    28741 gattattcgt atccgtcaca gtagtcgatt gccctaatac tgttttagcc gtactattat
    28801 ggaaggttgg tttcttctga taatcgtcaa tggcttgatt tatttttgcc tcaatggctg
    28861 cgatatttac ggcactacca tctgatcgtt ttattgagtg aagggtatac ccattaactt
    28921 cttgccagat catcttctgt gccacaatat gagtatctgc atctgtacct acttttttcc
    28981 ataaaacgga aactaatttg gctttatcgg acatatctgg taacgggttt ttctcatagc
    29041 caggagtgaa ctcattgtaa atcgggactc ctggttcaat acagaaaata tcttgtttaa
    29101 agccgttgta cactgcataa tgcggaacag tgacttctga cattggacga ccatcactaa
    29161 aagttccttc aacagcatat tgatgtgcta aatctagttc tacggttacc gtttgtggat
    29221 gggtaatttc atcagcaaaa gtaatcgtag gagcaattgc tccaacaatt gcttgaacag
    29281 ccaaactcaa aatagttaat acacataatt tctttttttg ttttttatac atgaaatgtt
    29341 ctcctttcaa attaagcaca aaaaaataag atgctcgctt gaacatcttt gtttataaag
    29401 tctttttaaa atttttattg actgaataga atggatttga gaatgaatga gcctaaaaat
    29461 ttaatctaaa atacgaaata tactgccttt aaattagaac acattttaga acacaaacat
    29521 ttcaaattta atcaaatata ataaaatata tttacgggaa aacctttata aatcaaggtt
    29581 tttcaaactt attcaatgat acgaagaaaa aaccaggtct taagaaagcc cgtaaagctt
    29641 cacagttctc aaaacgttaa ttgcgattat cgttcgagtt cacgagttac gagaatcgta
    29701 ctgggcgaag caatgctgag cgcagttacc ttaatcctaa cttttctggc actttccaca
    29761 atttggaaag tgcctttttt gtgtttttag ctatgttaat aggtaggatt cttggtctat
    29821 aatatcattc gatctataaa caaacgggaa aaagataaag tatcatcaaa caatattatt
    29881 tatccgcttc tgaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaatttaca
    29941 aaaattgctt ctcatgtttg ataaatattg tgacttgtga ccgaggagtt ggcaggagaa
    30001 caccgtttat tcggaagaag ggattgtgaa aagtcttttg tcataacctc ttatttggta
    30061 gctaattgta aatcccaaac ggcatgtatt atttttcata agtccgtttt taaggattaa
    30121 tgttttcttt tgctttttat atggtctggc ttcagcgaga agttccaacg catatacaag
    30181 gacgacttat gtcaacgata acaacgatta ccatggtggc cagtccactt ggattagcac
    30241 tgtttggtgc gctctttgat tggaagacaa cgcaaccacg tctgcgagac agtctaattt
    30301 ttctgggagg gagtcttgct atagttatcc ttataaccgg tctggtaaaa ttactcaagc
    30361 ttgatctgaa agaagcaaaa attttcagtg aagaggaata cagagaaacg actttggtcg
    30421 aaaagaaaga atcaggaaac tgatacaagc gtataacaaa ctgcctgata ataggaaaga
    30481 cacgtattgt ccgattgttt tttttgacat tcaattggaa aatttacact ttctttatac
    30541 atttctggct gctttcttta tacccgtcaa ctatacttca ctcatcaata agaaagaagg
    30601 ataagtatgg attttggaaa tgaaacggtc ttgaaagcaa ctggtataac caaaaaatac
    30661 ggtgctgcca aagcattaga taaagtatca atcgaaatca aacgtggaat gatctatgga
    30721 ttgattgggg aaaatggagc gggtaagtct acctttatga gaactattat gggtttgatc
    30781 tcgattgatg aaggttcgat cgaattattt ggaacgacgg atttgcaggc agcaagacga
    30841 cgtatgggtc aatcgattga aacccctgct ctttatccag aattaactgc aagagataac
    30901 ctacgtatcc aagcagcgaa cggcggtgtg tcggatagag aaatcgaaga cctgctgaaa
    30961 atgatgcgat tggaaaatac agggaaaaag aaagcaaaga atttctcttt aggtatgcgc
    31021 cagcgtttag ctattgcaaa tgcactgatc acgaatccag aatttttgat tttggatgaa
    31081 ccgacgaatg ggatggatcc agcagggatg gcagaaatgc gagaaatcat ccaacgtttg
    31141 gtaaaagaac ggggcatcac cgtcttgctt tctagccact tgctagatga attgtcacag
    31201 atcgccactc actatggtat tttgcatgaa ggacacttga tcaaagaatt atcgaaagaa
    31261 gaattggcac aagaatctcg tcaatttata aaaattgaca cttcggcgac ggaacaagca
    31321 gtaacggttc tcgactctct tggttatagg gattattttg tacaatctag ccgtgttatc
    31381 cagctttttg aaggaattga ccaagtagca gccatcaatc aagcattggt agaagcaaaa
    31441 gttcctgtgg atggtattca cctagttggg caaaaactgg aagattattt cttacaatta
    31501 actggaggca atccacatgt ttaatctatt aactgctgaa aaaataaaag tatggaagag
    31561 ccgtaaaatg tgggtctcac taggactgat ggtcgtactt ccgctttttt attcttggaa
    31621 cgaatggtat atgcatacaa aatacggcaa tgaattaaac caagcaacgg atacagtcat
    31681 caacggtgca acagggattt taatggtaga aaaaatggct gcatggattc ttctggcttt
    31741 cgcagcattt gcgtgctttt atattgggga agaatttcaa aacggtacga ttcgcaatac
    31801 gttatcttta ggacggaatc gaatgactta ttatgtgtcg aaacttctcg ttacgctttt
    31861 gatcacaaca cttggcgtgg ttttgatcac gggactagcc atgcttgctt acacacttgc
    31921 tttcggattt ggtgaagttg agggaatcaa aaactatgga aattatgttc taaaagtatt
    31981 tcctgtattg cttctgctga cattagggac attatctgtt ccagtggctt tgacattcat
    32041 cactagaagt accagtgtca gtttgcttct ttcttttctt tatatcatgg gaacggcctt
    32101 cattcccggt gttttcgcaa aaatcaaagg cttggaattt ctaacggagt ggtttacgga
    32161 aacgtggctc atgtatacgg actttgcgca acaagcaact tattcgcaag caccgaaaat
    32221 gattctggtc agtctagtaa caatcgtggt atcatcagct ctaggaatgt ttattttcca
    32281 aaaatctgac attaagtagg tggtcctgct ggaaactatt ttgattatcg aagacgatga
    32341 agctgttcat tccttactgg aggaggttct tgaacagaga tacaaacttc ttgatgctta
    32401 ttcaggtacg gaaggaaaat tattgttaag cacatatcct gtcgatttga tccttctaga
    32461 tctcatgctt ccagggcttt caggtgaagc attgttagca gaaatccgac agacatcgaa
    32521 tgttccgatc atcgttcttt cagcaaaaag cgatcaaaga gataaagttt ccttgttggc
    32581 agcaggagcg gatgactatg tgacgaaacc atttgatatc gaagaacttt tgttacgaat
    32641 cggcatccaa cttcgtcatc agacttctgt ccaagtaaaa gatctgtcgc aacgcatcta
    32701 ttacaaggaa atcttactgg acaaagagtc gagggatgta aatgtgaatg gtgtggctgt
    32761 ccatttgaca ggaagagaat tcgatctgct gaagcttttt ttagaacatc cgaaaaaagt
    32821 attttcgcga gcaaatcttt atgaaacagt ttggcaagaa ccatattttg atagtgagaa
    32881 aacagtgaat atgcatattt ccaatcttcg agggaagcta gcaaaggcac atgctgtgta
    32941 tattcatacg gtgtggggag tggggtttag atttgattaa atggctgttg cttctcgttg
    33001 ttatctttat gggaagtttt attttcttcc tgcaaaggga aatcaagaga ttaactaaga
    33061 aaatcgccac tctttctgaa cgacaagaat atggtggtcg tctttcagtc gaatttcgag
    33121 aaaagaatct aatggaccta gtagatgcac tgaatacatg gttggatgaa aatgaacaaa
    33181 aaggacaagc atttcaagaa atggaagaaa acatcagact atcgatcgtc ggcctttctc
    33241 atgatctcag aactcctttg actgctatta acggttatgt acaattactg gaacaggtag
    33301 aggatacgga gaaacgggag aagtatttaa cgatcatcaa acaatctgtt gcaagattac
    33361 tcagtatgac ggatcagttt tatgatcttg ctcgaataga gacgaagcaa aaagaaatgg
    33421 atctccatac gctttctttt tctaaagagg tggaagaagt atttttctct ttttatgaac
    33481 aattttctga gaaaaatatc gaggtagcgt tcgctgaaca ctcgataaat acaactgtat
    33541 tagcagatcc gctcatgttg ctgcgagtgc tgcaaaatat catacagaat ttattgcgtt
    33601 atgcagatgg acaagcaaag gtgacatttt caaaagagga tccattcatt cgattaacag
    33661 tggccaatcc tatcaaacca ggaacgcaaa cctctgttga aaaagtcttc cagcgttttt
    33721 ataccgaaaa taccagccgc acaaatacgg aatctagcgg aatcgggctt tacctgtcaa
    33781 aaaaattggt ggaaggaatg ggaggagaga tgaccgctag attagacggg ggcatcttct
    33841 ccatctccgt caagttgcgg agggtttaat tatacaagat tagtgagaac tagtagaaaa
    33901 atgtattatc ttatatatat ttaatagaga aaaaagcctg tgatataaat attgcaggct
    33961 tttttattga tttgaaataa tcaagaatat tagaaacttc tacattaatc aagaactttc
    34021 ttctgtatga aaatagagat aataattgta cacttaagct gtattcagga ggtgaagata
    34081 atgggcatcg aatttagcga acggcaaatg aaaatactat actttttgat tgaaaatgag
    34141 cacacaactt acaaagaatt atcagaacaa ttatatgtat ctacgaaaac aattagcaaa
    34201 gatattttaa ttttgaaaga aataatccaa aaaatgccag atgtacaaat gttggttcaa
    34261 cctaaaaaag gcgtttcgct tattggaaaa aaagaaacac ttcgaaattt attatccagt
    34321 cataaaatca atatcgcaga ctctccggag gaccgtctaa cttatgtgtt tgctaaatta
    34381 ttgaatacta atgattctct aaagattcaa aatcttgctg acgaattata cgtgagcagg
    34441 tcgacggtag aaagcacaat gaaagatgtt agaaaaagac taaacaagca tgggatgtcc
    34501 ttagtttcag atcgaaatgg cgtgcgcatc aaagctgatg aaaaaagtaa aagaaggttg
    34561 atgtcagaag ttgtgaacta ttattggggt ggctttacag catctaacag taaaacagat
    34621 ctcttagact tgaatatcaa tttcattggt gaagcgaata atttcttaaa taaggacata
    34681 ttgaaaaaag tagccgaatt gcttaatcga tacatgaata agacacaatt gaaatttaca
    34741 gattatgaat ttcaaacttt agctatccac cttgctattg cgatagaaag gattgaaaca
    34801 agcaatttct tatcacgtag taataattct gaagaagaaa tctcaaagaa tacacttatt
    34861 ttaatcaaaa tgattgaaaa agcattttca attattgtgc caatagatga acaagcttat
    34921 gttgacattc acattacttc gattgaaaaa aatgctatga atcacgactt agatcaaacg
    34981 aagctttcct atttagatat gggtgttgag ctacaagagt tagtaagaat taaattaaat
    35041 gaattttatc ctgatgaaga attgatcaat aatctagtat tacatctcaa tgcagcagtt
    35101 aaaagactca aattgggagt aaatatctat aacccttata cggacaaaat caaatattct
    35161 tttaaaaggt catttatgat aagtgtagat ttattagagg aaatcgaaga gagattctgt
    35221 atacatttta atgaagatga aatcgcttac gtaacactcc atattcaatc acttttggat
    35281 cgttacaaac ctgacaaaac gaaagtgata ttagtttgta gtagcggata tggaacatca
    35341 aaattattag agcaaagaat aaataacggt tttgcagata tggttgagat agaagatgta
    35401 ctaagcatca atgaactaca agatagaaaa gtcactgatg aacttgttat aagcacgatt
    35461 ccgattgaaa atacaaattt tcctgttatt gttgttagtc ctttgatgtt aggggcagat
    35521 gtcagaaaag taaaacagta tctacagtta aatgaacaaa cagaagatgc ttttctaaat
    35581 ttattagata aacgattttg cttttttacc caccaaataa aggataatca agtgaacgtt
    35641 ttaaatataa ttaccaaaaa attggtaaaa gaaaattacg cgaaagaagg gatactagag
    35701 ggagcaatca aaagagaaaa actatcttca acagcgatga atgattttgc tatgccgcat
    35761 atcgaagtag attttataaa aaagccagta atctgtgtct acgttaatcc agaaggaatt
    35821 gattgggatg ggacacttgt tcattctgtt ttcttttttg cacttaatga aaaagtaaaa
    35881 cctgaattga ataaaattta tgaaacattc aatgaaatcg cttcgaatca tgagttatta
    35941 gcaaatttaa aaacgagttc gtcgtttgaa aaattagttt atatattgaa agaaggctta
    36001 taaatgatca gtgaaaaaat caatataaat aatatagtga tagatttaaa tgcatctgat
    36061 aaaaaaagtg tagtcgaaaa attatcgaaa ttgttagaga ctaatgggtt tgtgtctgac
    36121 cacaaacaat ttcaaaaaga cgtatttgac agagaaaacc atatgaccac aggaattggg
    36181 aaaaatatcg ccattcctca tggcaaaagc ctagcagtaa ttgaatcaac agtggctgta
    36241 gccaagttaa agcggtctgt tgaatggggc gcattagatg atcaaccagt tgatctcgtc
    36301 tttttgttag caataaaaga cactgataaa ggtactaatc atttacgtat tttagcagaa
    36361 ttatctggaa gattgatgga tgacgagttt gttaaaagaa taaaagattc gagaaataaa
    36421 aaagaattgg taaaagctct atcattttaa aaggaggaat ttcatatgac aaaaatagta
    36481 gcagtaacag catgtatcgc agggattgcc catacttata tggcaaaagc taatttagaa
    36541 aagtttgcga agaagaaagg gttagatatt aaagtagaaa cacagggggc tatggggata
    36601 gaaaacaaac tttcaatttc ggatgtttcc gaggcagatg ttgtcatatt tgcagtagat
    36661 acaaatgttt cagaacgagg tcgctttgac ggtaaaccga ttttaacagt agggacctca
    36721 gaggtagtaa aagatggtga agagacgatt gatgaagcaa ttgcattaat taacaaataa
    36781 aaaatatatc gtgggggagt tactaatgga attcaagaaa caaggaagag aaattctaaa
    36841 tcatttccaa agtggggttt cctatatgat cccgcttgtc gtagcagcag ggttactaac
    36901 ttctattgca gtcatttttg gtggcacagg tgtttgggac cagacggata cattctgggg
    36961 agtcctgcga atgattggtc agacgggatt acagtttatc gtaccgatga tttcagccta
    37021 tatcgcatat tcaattgctg accgtcccgg tctagcacca gcttttatca ctgggatgat
    37081 gtgtcaaaat cttggtatgg gatttattgg aggaatggtt gctggattag cttgtggcta
    37141 tttagctaat gcattgaaaa aagtaaaagt accgatgcaa gttattagtc taaaatcaat
    37201 tatgctgatt ccttttgtta ctacattagt tgttggaatc atattatggt atgtactagc
    37261 tacgcctatt caagcaatga caacaggact gactaattgg ctagaaggca tgtcaggagc
    37321 aaatgctgct ttgatgtcgg ctattttagg aggaatgatg gcgtttgata tgggaggacc
    37381 tatcaataaa atcgcatatg cttttggggt tgcttctttt tcaagcggaa gctttggacc
    37441 tagtacagcc atgttattag gaattgctat tccgccattt ggaatgtttt tagcaacaat
    37501 tttaaacaaa aaactttatg atgaatcgga acgagataat gcaaagacag ctatgattat
    37561 gggccttgtg gggattactg aagggacgat cccgtttgcc gtgaaagatc ctttacgagt
    37621 aataccaagc atcgtggtgg gcactgcagt agcatgtggt ctaaatggat tttttggtgt
    37681 tactcaagaa acaatgcttt caacctttat ggcaattcct tttgttacga atataccttt
    37741 atatttagta tcaattgtta ttgggggagt ggttacagcg ttaatggtca atgcactaaa
    37801 atccatcaag tataaaaaag aacaacaagt aaattcagta gaagaaggaa agtaggagaa
    37861 tctgatatgg taaatgcaca tataattcaa cacacgcact gggacagaga atggtatttt
    37921 acaaatgaag atgccatcgt attgtctgat caggtcttta ctgaagttct agatgaattg
    37981 gaaaggaacc ctaaagtaaa cttttgttta gatggacaaa cttcaatcgt cgatgagtat
    38041 ttggagatca atccgcaaaa gcttaaggtt atccaacgac tgatgaaaac aggacaacta
    38101 ttcgtgggac cgtggtatgc acaaacagat gccttattag tggatgctga atcaatttta
    38161 agaaacttaa tgattggaat cagtgatacg atccttaaat atggagatcc aatgatggta
    38221 gggtatttgc ctgacacgtt tgggttcaat gcgaatttgc ctacactttt gcatcaagta
    38281 gaaatcgata atttcatgtg ttggcgagga ttaaactttg aaacgatggt tccatctcct
    38341 tattttatct ggaaaggatt gggagataaa tttgtttatg ccatgaattt tccatttggt
    38401 tatatgactg gaatgatgac cttagacgca attaaagata ttcctgaatt tgcaaaagaa
    38461 aagttggatg aagcagttga aatgcttcat atgcaaggaa aaaacaaaga tattttagta
    38521 ccttcgggtg tagatcaaaa gtcaatgatt ttaaattttg atcaagtcgt agagaagttg
    38581 aataaataca gtaaattcca caatattatt tcagattatc cttcttatgt agaagttatc
    38641 agaaacaaaa aagaattacc tgaatataaa ggtgaacttc gtcagccggt ttactctcgt
    38701 gttcatcgtt ctattggctc agtaagaaca aaaatgaaaa tagaaaattt taaattggaa
    38761 caaaaaattc taaaacgagt tgagccgctc atgctgattg cagaaagaaa tggtatccat
    38821 atcagtaaag gcctacttcg tcggttatgg aaaaagattt tgcaaaacca ggctcatgat
    38881 agtattggtg gctgtgtttc agataatgtt gcggaagata ttttccatcg aatcaaagaa
    38941 gctaatgaga tagctgacgg aatcgaaaac cttattgtaa aacggatggc tgatgcatta
    39001 aagctaaaat cgaatgaagt ccttgttttc aacacaacga ctgagaatta tagtggatcg
    39061 aaaatcgtgc atgtagtagc tgattcaaag aaaatacact tcaaaggtag tccctataat
    39121 atcattgaaa aagaagttta ttttccagaa cggaaaaatg cttatcggat gattgctaca
    39181 ggtttcgaat attttgatga accagcttat tatgaacttg acataaaaat aaatgtaaat
    39241 gtgccagcac taggatacac tgttgttgaa tttgagaaaa ataatgaaaa attagaaaca
    39301 gcaatcgaga tagacaaaac tgagataagt aacaataaat ataaactttc ttttaaagat
    39361 gggcaattga atttgatagt tggagataga caatatcttg atttcgttca tttgatcgat
    39421 tcagcaaatg atggcgatac gtatgattat tcaccattag aaggagatac agaactatct
    39481 ctaaaattag aaacagctaa agtatataaa gattctttac aagaaacatt agttgtttac
    39541 ggaaaagctc aacttcctaa agatttaaaa gatcgtttat ctgaaaaacc cgaaatggaa
    39601 gaaatatctt atgaaatatc gttcagtctg ggagaaagtc aaatcgttga aggcacactc
    39661 aaaattcata atactgttta ctcgcatcgt atgcgcttag caattaatgt tttagataaa
    39721 tctgaaaaat caattgcaca aattcaaaac ggatttattg aaaatacccc tgatgtcatt
    39781 cctccgaatt ggtcagaaac tatggtagag aaacctgtta atttagaaat attcgataag
    39841 agtgtctctg tggcagaaaa tgatcattat tttacgtttt atgcagatgg tttaaaagag
    39901 tatgaacgta tagaaaatca attaatcata accttatttt caacaacagg ggaacttgga
    39961 aaacctaatc ttgcttggcg acctgggcga gcttcaggag atacgacaaa tgaagggcat
    40021 gtcatgatgg aaacaccgct agctcaagag ataggtgagt ataaagtcac ttttggattt
    40081 aatgtagaag aaggaagatt taatgagttc aaagtggcaa aacaggcaga aaaacggtta
    40141 gaacaatcta tttcttatca aaagcagaaa ctgaatgtat ttattcacag gttagataac
    40201 aagatttggc cattacaaaa tgaacttgat gttcctcgta aattttcatt gattgaatta
    40261 cctgaagact taattgtatc atcgatttat ccttcttatt ttcatgaaca aggatttgtt
    40321 cttcgcttag ctaatccaac agaacaagaa aaaatagtac ctgaggctat cctttccctt
    40381 gggacagttg tcaatgcact ggagaataaa caagaattaa caacaattcc tccctatgat
    40441 tatttgtcaa ttttgataaa tgagagataa atatgtaata tacaagaaaa aaacaccttt
    40501 tattttgaaa ggtgtttttt taccctcttt tcaaatagcg caacagtgct atactaaagc
    40561 taacaaataa aggaacggag agggatatgg gtcaaagaga atttatcgtt ttaattatta
    40621 ttgcagctgt tattttatta gttgctttag acattttttc acgtttgaaa ttaaaggaaa
    40681 cagttcgtag caaatgggaa aaaattcctt atcaaccgag atttgataaa gaagaaagct
    40741 tgaaggaagc atggctcacg gagaagaagt tccgttcatg ggatagcgaa atcgatgatc
    40801 ttacttggta tgacctggat atgttcgaag tattcgaagg aatcaacagt acctattcta
    40861 gtgtaggttc agaagcgttg tatcaacgtt tacgcagctt tgattttggt gaagatcagc
    40921 agttagaaaa actgattgct ttttatcagg aaaatcctca actgagagaa aaaatccaat
    40981 atcaattcgc tcgtttagga aaaaaagatc ataattttgc aaaacaatat ctagcggatg
    41041 gaaaaagtaa aaagattgga cgcgtaggtt tatttatttt tcttggcttg cttccgctgg
    41101 tgggtttggg tttgcttctt ttaggacaag tactaggaat ctatttggca atcggtgggt
    41161 tagtattcaa tacggtctat tatctagtga agaaacaaac actagaaaca gagctgaata
    41221 gcatgagtta tttggtttca acgatttcag ccgccaatca gctagcaaaa ctgccgacac
    41281 cacttcaaga agagctgaaa cagcaaacta aaccattaag agacatccct aaattaggtt
    41341 tttcatttcg tatgaagagt gggagtgaag cagagttgat ttttgaatat gtcaacatca
    41401 tgttcatgct gccatttatc tcctatcatt tcgttctcac taaattagaa aagcattcaa
    41461 ctgaagccat caagctgtgg gaattgctgg gagaattaga agtagccgct gcaatattga
    41521 attaccgtac ttatatgccc atcacttgcc gaccagagtt tactgagggc ggtgtgacag
    41581 cgaaagatat ctatcatcca ttgttaaaag aagcggtagt gaatccggtc aactggcaga
    41641 agaatacatt agtcacgggt tccaatgcat caggaaaatc tacgtatgtc aaaagtatag
    41701 ccatcagctg tattctagca cagacgatcc aaacagcgat tgcagaacag tttaccctgc
    41761 aacctggaca tgtgttgact tccatggcaa tcgaagatga tttatttgaa ggcgacagtt
    41821 attttgtttc agaaatcaaa tcgatcaaac gtttgttgga ccaagtggct tccaaagaac
    41881 gctgctactg cttcgtagat gagattttaa aagggacaaa tacgatcgaa cggattgctt
    41941 cttccgcttc agtcgttcgg tggcttgcgg agtatccatc tttagcattt gtcgccacac
    42001 atgatatcga attgacagag attttaaaga attattgtga gaatgtccac tttgaagaac
    42061 aagtaacaga aggaaaagga atcagttttg atttcaaatt aaaagaagga ccttcaagga
    42121 cccgaaatgc gattgccttg ctgaaagttt tggattaccc agagacttta gtagaaaccg
    42181 caaaaacgga agccgcgtta tttgatgaaa agcggcagtg gcatgtattg ggataggcga
    42241 agtgatcgaa agtatttatt ggatcaaaaa tcttggcttt tgcttccgcc gtttatacgt
    42301 gtttagagcg tgagacaaaa gtgttcttta cttttgtctc acgctccttt tgttgcacat
    42361 atgaatagta atcctttatc ttcgatttgt tttttctgaa aaggttgtct gacttcgtcc
    42421 gattgatgtc tttcattagg agattttcct gagcccagca acctgatttt tcgctggaac
    42481 caagttgttt tttcatctgc tgtattccat gatacaataa agaatcatgt ctataaggag
    42541 ataggaaata aatggcaaat gcgcttgaaa taaataattt gaaaaaggtt tatggaagtg
    42601 gggtcgaagc attgcgagga atcgatcttt ccgtgaaaga aggagatttc tatgcgctat
    42661 taggaccaaa tggagctggg aagtctacaa cgatcggaat cattacttct ttagtcaata
    42721 aaacatctgg aaaagtgaat gtcttcggtt atgatctgga tacggatcta gtccgagcaa
    42781 agcaacagat tggattagtc ccgcaagagt tcaatttcaa tccctttgaa acggtccagc
    42841 aaatcgtcgt gaatcaagca ggctactacg gtgtctctcg caaagaagcg atgaaacgca
    42901 gcgaaaaata tttgaagcag tccaatcttt gggaaaagag aaatgtccgt gcacgtatgc
    42961 tttccggggg aatgaagcga agactgatga ttgctagggc attgatgcat gaaccacgtt
    43021 tgctgatctt ggatgaacca acagcaggag tcgatattga attgagacgc gagatgtggg
    43081 agtttctccg agaattgaac gaaaatggta caacgatcat tttgacgacg cattatttag
    43141 aagaagctga aatgctttgt cgcaacatcg ggatcatcca atcaggtgaa ctgatcgaga
    43201 atactagtat gaaagagctt ctatccaaac tgcaatttga aacatttatc tttgatttag
    43261 cgccatatga gaccaaacct gtgattgaag gatacgcttg tacatttgaa gatgaacaga
    43321 caatcgctgt cgaagttgaa cgaaatcaag gggtgaacgg aatcttcgat cagctgacaa
    43381 gacaaggaat caaagttctt tctatgagga ataagtcgaa tcgtttagaa gaactgtttt
    43441 tgaaaatcac agaagaaaat aatcatacgg aggagaaaaa tgtttaatct ttattttact
    43501 gcattaaaaa gtttagctat caaggaaacg aaccgctatt tgcgtatctg ggtccaaaca
    43561 ctggttccgc ctgtgatcac gacatccttg tactttgtta ttttcggaaa aatgatcggt
    43621 ggtcggatcg gtgacatggg tggtttttca tatatggaat tcatcgtgcc tggtttgatt
    43681 atgatgtctg ctatcactag ttcgtatgcc aacgtttctt cgtccttctt ctctcaaaaa
    43741 ttccagaaaa acatcgaaga aatcttggta gcgcctgttc ctacgcatgt gatcatttgg
    43801 ggctttgtga ttggcggagt gggtcgcagt attctcgtcg gctctctcgt aacgatcatt
    43861 tccttgttct ttgtaccact tcatgtgtat tcatggttca tggtcatcat tacattattg
    43921 atgacggcaa tcttgttttc gttagcagga ctgctcaacg ggatttttgc tcagtcttat
    43981 gacgatgtat cgatcgtgcc tacatttgtc ttgcagccgt taacttatct aggtggcgtt
    44041 ttctatgcca tctctatgtt gccgccattt tggcaagctg tttcgaaagt caatccgatc
    44101 gtttatatga tctccggttt ccgttacgga ttccttggaa caatcgatgt gccagtagca
    44161 acatcgatga tcgtacttgt gctattcatc gttgttttgt acgccgttac ttggtatttg
    44221 atcaataaag gaagaggatt gagaagttag aaaagaggtt gtgaaatcag cgttttgacc
    44281 tgagtattag agaaacaaac gggaaaatag ttcctcatat tttgtcgttt gttgagctaa
    44341 taccgagggt cagctgattg aacaccgttt agtggagagg ttgtgaaaaa gctgttctgc
    44401 atcaagtaat aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttggact
    44461 tattaccgca gatgcaagct tttgaacacc atttattccg gaaaaggaat tgtgataaga
    44521 tttttgtcac agtctttttt tgctcaatca gaaacatctt ttaagctgtt cactttgctt
    44581 ctttttttgt ttcctagtat aatgacaatg tttttgagaa agattgcgac ttcactgctt
    44641 gatagaatgg agagtaacaa ataaatgact actaattttt ggaaagagct gccaaagcct
    44701 ttttttgttt tagcaccaat ggaagatgta acagatgttg tatttcgcca tgtcgtaaag
    44761 catgcagcag caccggatgt cttctttact gagtttacaa actcagacag tttctgtcat
    44821 ccagatggaa aagacagtgt acgaggaaga ttgaccttta cagaagatga acagccgatg
    44881 gttgcccata tttggggaga caagccagaa ttttttagag aaatgagcat tgctatggca
    44941 gaaatgggct tccaaggaat cgatatcaac atgggctgcc cagtccctaa tgtagcagaa
    45001 cgtggaaaag gcagtggctt gatccttcgc ccagaagtag cggcacaatt gattgaagcg
    45061 gctaaagctg gcggtttgcc tgtcagcgtc aagacgcgta tcggctatac ggaaatgtct
    45121 gagatggaag aatggatcag tcacctactt catcaagata tagccaatct ttctgtccat
    45181 ttgcgtacgc gtaaagagat gagtaaagta gatgcacatt gggaagtcat ccctaaaatc
    45241 attgctttac gtgaccaaat cgctccgcaa acactgatca cgatcaacgg agatatccca
    45301 gatcgccaga caggagaaga attggccgaa aaatatggcg tagatggcat catgattggc
    45361 cgggggattt tcaagaatcc ttatgcattt gaaaaagaac cgagggaaca tacagaaaaa
    45421 gaactgatcg gactacttcg tctgcaacta gatctgcaag atcattattc agaaatcatc
    45481 ccgcgttcca tcacaggact acatcgtttc tttaaaatct atatcaaagg atttccagga
    45541 gcaaatgact tgcgagttca gttgatgaat acgaaatcta ctgatgaagt acgagagatc
    45601 ttagacacct ttgaaaaaga acatccaacg cttttcagtg aacaatgatt tcttgcttga
    45661 acaccgtgaa ttagaggtcg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
    45721 caaaaatagc tcctcatatt ttgtcgtttt ttgagcgaat accgagggtc agttgattga
    45781 acaccgttta aaagaggttg tgacaaaagt cttgtcgcaa cctctttttt aaccaaaaaa
    45841 gtgcaaatag tttactttaa gtaagagaat aattatgatg aataaaagtg gaacaaatag
    45901 catactatat atattgaaat gaaaactgta tggatgctcc aacttgatga agctaaggag
    45961 gaattcgtat gtcagaaaag gaattaatga aggtagtcgg tttttatgaa gggttgtcga
    46021 tcgatgatcc aaaaagcttt attgatgaaa aagaacagat acctgtaccc agttcacggg
    46081 atcttctggt aaaagtcaac gcagtatctg ttaatcctgt cgatacgaaa cttcgccaag
    46141 ataacgggat aagaaatgct ttacgtatct tagggtttga tggagtcggg aaagttgttg
    46201 ctgtaggtga tgaagtgcaa aaattttctg tcggcgatcg cgttttttac gcaggtacaa
    46261 cgacaagagc aggaagcaat caagaatacc aactggttga cgaacgtatt gtcgctcttg
    46321 caccaaaaaa tctttcagat gaagaagccg cagccttgcc gttgacctca ttgaccgctt
    46381 atgaattgtt atttgaaaaa tttggtctga ctccagaaga aaatgccaac cgcgggaaaa
    46441 aaatcttagt gatcaacgga gctgggggcg tcggttcgat tttgaatcaa ctagcgcatt
    46501 gggctggcct agaagtctac gcgactgcaa gtccgaagaa ttttgaatgg ctgaaaaaaa
    46561 caggtgtaga ctatccaatc gattatcatc acgacttaag taaaagtttt cgtgaattag
    46621 gaatcgatca agtagagtat gtcgctgtat tattcgatat tacccgctac tttgatcaaa
    46681 tcaaacacct tgtccgacca tttggacatg tgggaacgat cgtagggatc aaagagccgt
    46741 tagatatagg tgtttggaaa aaccaatcta tcagcttcga ctgggagtat atgttcgcta
    46801 aaacagacta taactatgaa atcgaaacgc aaggagaagc tttagcgcac attgctgctt
    46861 tagcaaatga agggattatc cgcagtacat tgagcaaggt ctattcagat gggatgaatg
    46921 cgaagaactt gaaacaagca accaaagacg tagaaacagg acatatatta ggaaaagtcg
    46981 ttgttagtgg tccgttcaac ggataagcaa gaatacgccg atttttttct tatttgactt
    47041 tgataaaaac aagagcgcat gacagtaatt tgccataagc tcttgttttt ttatttataa
    47101 taagcgaatc aacgaaaggg aaagaattcc taccaagacg atcaccaata agctttttga
    47161 aagaatagca gctaaaagtg taggaactga agccaaagca ttttcgagat tgatctgcgg
    47221 cagatgtccg atattttggg taaatagact gctgaaccat aaagcagaca taatgacgat
    47281 cgggacaaaa cttaaatact caagcaacgg ttgcggcaaa tcgaattttt tcagtaagac
    47341 aaatggtagg atcctagaaa gccaagttgc taaagtacaa cctaaaatag tcagcaatgt
    47401 aaactcaaaa gaaggcatgt ttgatccaca cccccaatcc gcacccaatc agtgttacaa
    47461 ctaaaatcac taagttactt gggataaaaa tcagtccgac atataccaga atcaaagtga
    47521 gaccaatcag gatcaactgt aatcgtttag aggtgtttct atcagaaatg acttgaagat
    47581 aaagtaaccc gataaacata gcgacgatgg cgaaatccaa accaaacttt ttcggatcag
    47641 taataaaatt ccctaaaagt gcgccaacaa gagaagaacc cacccacgtc aaataagaaa
    47701 tccaattagc tgtattcatc caacgaaagt ttaacttttg tccagtatag ttcaatttat
    47761 tcattcccag tgcgaaactt tcatctgtca gcaatgtgcc aatcagcaga ttttgaagca
    47821 gccgattttt cttgaaataa ggagcaatcg tcatactcat cagaatcatt cgggaattga
    47881 ctagaaaggt cgagagaacg atggagagca gtggactatg gctagcaagc atgctgaccg
    47941 tgataaattg ggcagaacca gcatagatca gcagggacat caacgtgacg accagcggat
    48001 gaaacccggc agctttccca acgataccaa atgcaagtcc aataccgata taaccaaaaa
    48061 cggtaggtaa cgtgtctttg atcgcagttt ttatatctaa cttttcatcc aataacaaaa
    48121 ctcctttagc acgacataaa ttttcataat tgtcgtcaaa cttgtgaata ttctaaattt
    48181 ttaatgaaga cttgtcaact tcaattttaa acaaataaaa aagactggac gtagtttaaa
    48241 aaaatcttct caagttagat ttccagacct aacttctgag gatcactaca tttgtcacag
    48301 tctcttatac tgacaagtat aaatgaagta tgaagaaaaa gtatttgtta ttggtatttc
    48361 tcattattct tgttttctaa tgtgttcact tcaagtggaa ttttagaata ttattcaact
    48421 aacaagtcta catcgtatct aaatccattt gtatcactta attgcttgta aaattcgcca
    48481 ctttttttaa ttgttctttt ttgagtagta aggtcgagtg aaaccagccc atatcggttt
    48541 ttataggcgt tgatccatga ccagcaatca ataaatgtcc aaagatgata ccccttaatg
    48601 ttacatcctt catttattgc cttccataag taagcaagat gatctttgat atactcaatc
    48661 cgataatcat caattatttg ccctgtatca tccatgaaac gttcttcatc cgcaacgccc
    48721 attccgttct ccgagataaa tgcttcgatg tttccataat ggttcttgat atccatgcac
    48781 aaatcataaa ttcctttttc gtaaatttcc catccccggt attcgttcat tcgtcgtcca
    48841 ggcatgatat gtggttcgaa aaaccactct ggtaaaaatg gactagatgt atccaccata
    48901 gaagaacgag atttgacgcg acgtggctcg taatagttta gtccaagaat ttgagcggta
    48961 ttttcgtaga tacatacaag atcttctttc gtataatctt caggaagctg attatattct
    49021 tgtaataaca ggataagctt tttagaaaaa ttccctttaa ccataggatc taaaaagctc
    49081 cgcgtataaa ataaatcagc gatttctgct gctttcacat cagcaggatt ttgactacga
    49141 ggaaaagtag gactcaaatt tagaataacg cctattttac taggaagagc tagttcttta
    49201 aatacttgta ttgcttttgc gtgtgcaagt atagtgaaga atgctgcttg cgctccgcgt
    49261 ttaaagtcaa tgaggtttgg ataatgaaaa tcttcaagat aagtacctaa aataggccca
    49321 agtggttcat tgaaggtaaa ccaatgacaa actaagtcac caaattcttt aaaacaagtt
    49381 tcagcgtaat gaacataagc atcaactact tcacgtgatt cccatcctcc tttatcttga
    49441 agagtagcag gcatgtcaaa atgatataag ttggcaaata ctttgattcc gttattattc
    49501 atttcggaaa ataattttcg atagaaagaa atggctttgg gatttacttt tccggtaccg
    49561 ccatttggaa acattcgtgc ccaagaaata gaaacgcgaa aggagttatg cccagttttt
    49621 ttcattagtt ctatatcttc tttatattga tggatgtgat cggttgtaat agaggaatcg
    49681 attcgttgat aaaaccggta aggttgttct ttaaaccagc gatcccagat agtctctgct
    49741 tttcctgttt ctccttttcc ttctgcttgc tcagctgacc aagcggaacc ccaccaaaaa
    49801 tcatctggaa aagttagctt tttcatagtt gtcataattt ttttacctcc tgtttccact
    49861 taatgataac accgcttaca acttaaagta aatagttttg tttaaatata tatatctttt
    49921 ttattgtgtg tataattttg atgtggattt tatcggttta tttatgttaa agtatagatg
    49981 aagatatgtt tttgattgga gggtcaccat tgaaaaaaat tgcgaagtat atggaaattt
    50041 acttagatat aaagaaaaaa attgtaaatg gagagtataa aattggtgag aaacttccta
    50101 caggagatga attggcagtt atttatcata caagcaagtt aacagtaaag aaaggcgtag
    50161 acttacttgt ttccgaagga gttttgcgaa gtagaagtgg gtatgggact gaagtcgtca
    50221 gaacaccaat tgataactca cgagttcttg gtccaaacga aggtttgttt agcgtggttg
    50281 gtgaagaaca cgtagattca gaaatccata ctttctcaat tgaacttcca tcgaaaaaag
    50341 tagctgataa attaagtatc agtgtaaaag actatgtata taatattgta cgaagccgtt
    50401 ttattgattg tcaaccatac tcgattgagc aaacattcat gccattagca gtcataccag
    50461 gcttggaacc aaaacattta aagaaatctg tatatgctta tatcacaaat gaactgggtc
    50521 tggaaataaa gacttcgcac atacgtatca aaggagatac tgcgaatgaa tttgacgcaa
    50581 aaatattaaa aatagatcca ggtgaattta tgattgagat agacaaagtt gttgctctat
    50641 cgtctggtaa acctttcgag tattcggtat cgagacattt atatcaagac tttgtttttg
    50701 aagctgtttt tgttgaaaaa taagctcggg aaaattattt tttcagagct tattttttta
    50761 tactaaaaaa gatatatata tttaattaaa agtatttact ttttttgata agtgatgtat
    50821 tatttgggtg gaaaaagtaa tcgattacaa taagcataaa ggagaaaaaa tatgggcaaa
    50881 agtagcagga aggacattgt atttgaaaaa tttggtatag ttgcgactaa gttggggaat
    50941 cagatccatt taagaacttt gcgagatgcg tttgctacat ttatgccatt tatgatgtta
    51001 gcaggatttg tgacacttat taattcagtg atcttagaac caacaggatt catgggggaa
    51061 attgtcgata ctagtacatt gaccacgtta caacaaattg gtacttcaat tgccaatggt
    51121 acattaagca taactacatt attagttgta gcagcagtat cttatcatat gtgtgcaagc
    51181 agaaattata caaatcatat tgcagcggta ttagttgcta tttcgacatt tgttgtatta
    51241 acacctatgc agatgatgtt tactcctgaa aatgcggata aagctgttga agtgacgggt
    51301 gtgataccta tatcctatac aggagcttca ggaatgtttg taggtatatt tgttggatta
    51361 gcggctacag aattatttat taaactttct aataataaga aactacagat tcagctatcc
    51421 ggaaatattc ctcccgctgt tttaaagtct tttaatgtat taatcccaat cattattacg
    51481 attacctcgt ttgctttat
//
LOCUS       DAYYFD010000007        45798 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000007 DAYYFD010000000
VERSION     DAYYFD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.007"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..2306
                     /locus_tag="ACHA25_000385"
     CDS             141..2306
                     /locus_tag="ACHA25_000385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8761718.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2610..3443
                     /locus_tag="ACHA25_000386"
     CDS             2610..3443
                     /locus_tag="ACHA25_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8761719.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3545..5245)
                     /locus_tag="ACHA25_000387"
     CDS             complement(3545..5245)
                     /locus_tag="ACHA25_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761720.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            5752..6912
                     /locus_tag="ACHA25_000388"
     CDS             5752..6912
                     /locus_tag="ACHA25_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8761721.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6928..7371
                     /locus_tag="ACHA25_000389"
     CDS             6928..7371
                     /locus_tag="ACHA25_000389"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8761722.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(7854..8384)
                     /locus_tag="ACHA25_000390"
     CDS             complement(7854..8384)
                     /locus_tag="ACHA25_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8761723.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            8489..9040
                     /locus_tag="ACHA25_000391"
     CDS             8489..9040
                     /locus_tag="ACHA25_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8761724.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(9193..9666)
                     /locus_tag="ACHA25_000392"
     CDS             complement(9193..9666)
                     /locus_tag="ACHA25_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8761725.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            9804..11276
                     /locus_tag="ACHA25_000393"
     CDS             9804..11276
                     /locus_tag="ACHA25_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8761726.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            11273..11983
                     /locus_tag="ACHA25_000394"
     CDS             11273..11983
                     /locus_tag="ACHA25_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8761727.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            12335..12619
                     /locus_tag="ACHA25_000395"
     CDS             12335..12619
                     /locus_tag="ACHA25_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8761728.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(12936..13319)
                     /locus_tag="ACHA25_000396"
     CDS             complement(12936..13319)
                     /locus_tag="ACHA25_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8761729.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(13321..13905)
                     /locus_tag="ACHA25_000397"
     CDS             complement(13321..13905)
                     /locus_tag="ACHA25_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8761730.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(13952..15427)
                     /locus_tag="ACHA25_000398"
     CDS             complement(13952..15427)
                     /locus_tag="ACHA25_000398"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8761731.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            15608..15919
                     /locus_tag="ACHA25_000399"
     CDS             15608..15919
                     /locus_tag="ACHA25_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761732.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(16034..18370)
                     /locus_tag="ACHA25_000400"
     CDS             complement(16034..18370)
                     /locus_tag="ACHA25_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8761733.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            18512..19180
                     /locus_tag="ACHA25_000401"
     CDS             18512..19180
                     /locus_tag="ACHA25_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8761734.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            19183..20226
                     /locus_tag="ACHA25_000402"
     CDS             19183..20226
                     /locus_tag="ACHA25_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8761735.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(20463..21344)
                     /locus_tag="ACHA25_000403"
     CDS             complement(20463..21344)
                     /locus_tag="ACHA25_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8761736.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            21503..22231
                     /locus_tag="ACHA25_000404"
     CDS             21503..22231
                     /locus_tag="ACHA25_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8761737.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            22387..22704
                     /locus_tag="ACHA25_000405"
     CDS             22387..22704
                     /locus_tag="ACHA25_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8761738.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            22697..23305
                     /locus_tag="ACHA25_000406"
     CDS             22697..23305
                     /locus_tag="ACHA25_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8761739.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            23360..24151
                     /locus_tag="ACHA25_000407"
     CDS             23360..24151
                     /locus_tag="ACHA25_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8761740.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            24289..24777
                     /locus_tag="ACHA25_000408"
     CDS             24289..24777
                     /locus_tag="ACHA25_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8761741.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            24828..25700
                     /locus_tag="ACHA25_000409"
     CDS             24828..25700
                     /locus_tag="ACHA25_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8761742.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            25876..26238
                     /locus_tag="ACHA25_000410"
     CDS             25876..26238
                     /locus_tag="ACHA25_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8761743.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            26247..26999
                     /locus_tag="ACHA25_000411"
     CDS             26247..26999
                     /locus_tag="ACHA25_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8761744.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            27013..28815
                     /locus_tag="ACHA25_000412"
     CDS             27013..28815
                     /locus_tag="ACHA25_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8761745.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            29092..30132
                     /locus_tag="ACHA25_000413"
     CDS             29092..30132
                     /locus_tag="ACHA25_000413"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8761746.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(30184..32328)
                     /locus_tag="ACHA25_000414"
     CDS             complement(30184..32328)
                     /locus_tag="ACHA25_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8761747.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(32685..34058)
                     /locus_tag="ACHA25_000415"
     CDS             complement(32685..34058)
                     /locus_tag="ACHA25_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8761748.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(34058..34453)
                     /gene="rbsD"
                     /locus_tag="ACHA25_000416"
     CDS             complement(34058..34453)
                     /gene="rbsD"
                     /locus_tag="ACHA25_000416"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8761749.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(34481..35398)
                     /gene="rbsK"
                     /locus_tag="ACHA25_000417"
     CDS             complement(34481..35398)
                     /gene="rbsK"
                     /locus_tag="ACHA25_000417"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8761750.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(35506..36519)
                     /locus_tag="ACHA25_000418"
     CDS             complement(35506..36519)
                     /locus_tag="ACHA25_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8761751.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(36600..37199)
                     /locus_tag="ACHA25_000419"
     CDS             complement(36600..37199)
                     /locus_tag="ACHA25_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8761752.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            37385..38086
                     /locus_tag="ACHA25_000420"
     CDS             37385..38086
                     /locus_tag="ACHA25_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8761753.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            38096..40807
                     /locus_tag="ACHA25_000421"
     CDS             38096..40807
                     /locus_tag="ACHA25_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8761754.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            41258..42886
                     /gene="treC"
                     /locus_tag="ACHA25_000422"
     CDS             41258..42886
                     /gene="treC"
                     /locus_tag="ACHA25_000422"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8761755.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(42946..44127)
                     /locus_tag="ACHA25_000423"
     CDS             complement(42946..44127)
                     /locus_tag="ACHA25_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8761756.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            44379..>45798
                     /locus_tag="ACHA25_000424"
     CDS             44379..>45798
                     /locus_tag="ACHA25_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8761757.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT        14638 a         8113 c         9344 g        13703 t
ORIGIN      
        1 aaattataga agggaacaca cgtattatag gttttataga atggtataga cagaaaaata
       61 aaaaataaag catttacaaa aaagaaaacg cacgctataa tacgtttaac gaaaccgatt
      121 gcgtaaagga gctaaacgga atgaaaaaga tatttagttt tgagttttgg caaaaatttg
      181 gaaaggcttt aatggttgtg gtagcagtta tgccggcagc tgggttgatg atcagtatag
      241 ggaaatcaat tcccttgatc aatcctgatt ggacgccgct tgtcacaacg ggcggagtaa
      301 ttgaaaatat tggttgggca attattggta atcttcatat tttatttgct ttagcaatag
      361 gaggaagttg ggcaaaagaa agagcaggcg gagcatttgc agctgggatc tcatttattt
      421 taatcaatcg gatcactggg gctatctttg gtgtcacaag tgacatgctt gcgaatgaag
      481 atgcatttac ccacactctg ttcggtacga agatcatggt taaaggattt tttacaagtg
      541 tattggaagc accagctttg aatatgggag tattcgtagg gatcatcgct ggatttgtcg
      601 gggcaatggc ttataataag tactataatt atagaaaatt gccggatgct ttgtcatttt
      661 tcaatggaaa gcgctttgtt ccgtttgttg ttatcttatg gtcaacaatc gtggcattga
      721 ttcttgctgt ggtgtggcca aacgtacagg ctggaatcaa taattttggt ttatggattg
      781 cagaatcaca agaaagcgca ccgattctag caccgttttt atttggaaca ttagaacgtt
      841 tactattgcc atttggattg catcatatgc tgacgattcc tatcaactat acacagcttg
      901 gcggatcgta tgagatttta tcaggcgcac aggcggggac acaagtattt gggcaagatc
      961 cgttatggtt ggcttgggcc actgatttag tgaatcttaa aggtgcaggg gatatgtcgc
     1021 aatatgagtt tgtgttgcat aactggacgc cggctcgttt caaagtagga caaatgattg
     1081 gttcttcagg tatcttgatg ggaatggctt ttgcgatgta tcgtaatgtc gatcctgata
     1141 agaaagcaag atataaatca atgtattttt cagcagcgtt ggctgtcttt ctaacaggtg
     1201 taacagaacc attggagttc atgtttatgt ttgctgctgt gccattatat gtcatttatt
     1261 cagtgatcca aggggctgca tttgcgatgg cagatatctt gccattgcgg gtccattcat
     1321 ttgggaatat cgaattgctg actcggacac cgttagctat caaagcgggt ctaggtggag
     1381 atcttatcaa cttcgtgttg tgtgtgatcg cttttggtgt tgttacgtat ttcttagcaa
     1441 atttcttgat caaaaaattc aactttgcta caccgggacg taacggaaat tacgacaatg
     1501 acagtgaaga aaccgttgta tctaacagcg gaacaggaac agcagatcag caagtggtcc
     1561 agatcattca tttgctggga ggaaaagaaa atatcaaaga tgtcgatgct tgtatgactc
     1621 ggttacgcgt aagtgtaaat gatcgagaaa aagtagggac tgaagaagcc tggaaacgag
     1681 caggggcgat gggattgatc gtcaaagaca atggagtcca agcagtttat ggaccaaaag
     1741 cagatgtctt gaaatcagat atcgaagatc tgctgcaatc aggagcagag attccagagc
     1801 cagaaatggt ttctacagga aatggacaag caggtagtaa gcaatactta ggtatagaac
     1861 aagaactagt ttccgcagct tctggagaag tgattccttt atctgaagta aaagatccgg
     1921 tattctcaca aaaaatgatg ggtgacggct ttgctgttat tccaacatca agagaagttg
     1981 ttgcgccgat cgctggaaaa gtgacaagta ttttccctag caaacatgca attgggctgg
     2041 aaacgaaaga tggtatcgaa gttttgatcc atatgggaat cgatacagtc caaatgaatc
     2101 agccagcttt tgagattgct gtcaaagaag gccaagaagt aggaacagga acgaaattag
     2161 ctgagatgga tcttgatgtg atccaaaacg aagaaaaaga tccaacgatc atgatcgtat
     2221 ttacaaacga taaagtagaa gaagtagtga tcaagcaatt aggtacagtg acagctggta
     2281 aagtgatcgg ctccattaaa atgtaaggag aggttgtgaa aaagctgtcc tgcatcaagt
     2341 cataagaaca gccaaacaaa aatagctcct cctatttttc gtttggttgg gcttattacc
     2401 gagaagcagc agtttgaata ctgtttagtg gagaggtcgt gaaaaagctg ccctgcacca
     2461 agtgataaaa acagccaaac aaaaaatagc tcctggtcca ttgagcttat tactgaggag
     2521 ttagcaggag aacactattt gctggagagg ttgtgacaaa aattttgtca caaccctttt
     2581 gtttcgaaat gaagacaagg agaaaaccaa tgaaattcat gacactcaac acgcatagct
     2641 ggcttgagcc agaaccagag aaaaaattgc aagaattagc agataagatc ctgttagaag
     2701 attatgaagt gattgcttta caagaaatca accaattact ggaatctgaa gaagtagaac
     2761 ctgcgaagct aatgaagttc tgtccggtaa agaatcaagt acctatccgt aaagataatt
     2821 ttgcctaccg tttagttcag cttttaaagg aacacggaaa agagtatttt tggagttggg
     2881 aaatgagcca catcggttat gataaatatg aagaaggaaa tgcactgctt acgaaaaaaa
     2941 cattggaaag tcaggtcctt accgtctccc aatttcaaaa gaaagaagat tatcagacac
     3001 gcaagatctt gattgggaaa acaaagatag atgatcagga catttttgtc tcgagctgcc
     3061 acttttcttg gtggacagat aaaaaaagtg gcttttattt tgagtggaaa aacttggaaa
     3121 actacttttt ggaaactcgc gtaccacttt ttttcctagg tgatttcaat aatccagtag
     3181 actctcaagg ttattatact gtaagagaaa gttgccttct tcttcaagat tcgtatgttg
     3241 tcgcgaatga aaaaggaaaa gcagctactg tggaaaagaa aatagatggt tgggaacaaa
     3301 atacagaaaa attgcgtata gattttattt tcgtccctga aggaatgcag gttaaaaaat
     3361 atcaacggat ttttgatggg atcgatagtc caatcatcag tgatcactac ggggtggaaa
     3421 tcgaattaga tgtgaacgaa taaaaaatat aacatataac ataaaaaaac tgattaaaat
     3481 aaaactctcc aaacgattat accgtttgga gagttttttg atcggatgta tttatccctt
     3541 ttgcctaagc acgtaaaagc ttgtatctat gataaataaa taaacatagc aaggtaaacc
     3601 agccaatcaa agtaagagaa agaatgaaat agaaatagat tggcgtttga taatgaagcg
     3661 tgagctcgtt gattcctttt tgctgcttca cgattggggt cccgatatct gttacttcat
     3721 aatcagtcaa tgtttttcca ttttgctgta gcacagtccg atcgtagacg ataacaggga
     3781 tatgaacagt tttgtcccca gtgtttttcc acttgattgt caaaagaccg cctggatgtg
     3841 tttttataaa agttgtttcg ttttctagta tttcttcgtt ataccggtcg tatttattgt
     3901 cttttgtctc cttatagatt ggcagatagt caggtgtggt tttttggaaa acgaacaagc
     3961 tggtagacat atctttgctg aagaatgttt tccttgcttc ttccggcgta tcgaatagat
     4021 aagtatgtcg actgacaaat gtttcatttt cccacttttc cagatgctgg tataagttct
     4081 ggatcgtctg tgctgaaaaa ataagaatgc caaatatact taaaagacgg aaccactttt
     4141 gttcagacca ctggttcagt gaaagggcaa ggtaaagtag aagcaatagc gtaaatggca
     4201 cgaaaaatcg aaatggaaac tggatcaaac taataaaagg cacatttttt tcgatcaatg
     4261 tcgtccatgg aatcaagcta gtagaaagaa aaaagaaaac aagacttgtg tacaacacga
     4321 ttttttggaa taaggtgttc ttttgttgaa agaaaataaa aagcaaaaaa cctgataata
     4381 tcaatatagg aaaggcctta gggtaatgga gccaataact gccatccaga tctatcgcct
     4441 tttgactcat ttctctgtta acaaagggag gaagaatctt attcccactg taaacaatag
     4501 ctaatcctgc ccaaatattt gctgtcagaa caaaataaag caagaccgac ttaatcagtt
     4561 gaagcagtgc ttttcctttt tcagtctggt taaaaaagta ataaagataa aaagggatat
     4621 acatcaaagc taacatgatc gaagacagta aatggatctg tgtcataagt cccactgaga
     4681 cagcgacttt gataacaggg aactttttat gaaggacgaa atcagcaata gggatcatac
     4741 ataaaggata aaaacaagtc ccccagctag tgaatccttg gttgatagac cagtattgaa
     4801 tagaatacgt actcatgaat aaaacggcta ctggtacact tacccaattt cgaacattga
     4861 catatctcag taaaatcagc atagaaaatc ctgaaataag ataaagaatg aaatttgaaa
     4921 gaatctgata atgaaaccag ctacctgcaa gcaacacgat caatccttgt aaataagcga
     4981 aaaatgggcc gtaaacggca ttcacgatcc tgccactttg ctggaatcca taaagggaag
     5041 gaaaataatg gaaatcccca tatttaattt gttgcgcggc atcatagaac cgattgtaat
     5101 ggaatggggc atcagcaccc aaaattagtt tatgtgtgaa taaatgaggc atgagaaatg
     5161 ccaaagcaat gaaaaaaatt gctaaaaatg ggccatattt tttcaattgt cgaactcctt
     5221 tgcaaacttt agataaattc atcatatatc ctatgcgaga ggtaggcaag aaattagaaa
     5281 attcatgaaa atatttatga attttacaag aaaaacataa gtgaaagttc taaggagaga
     5341 ggatgcgaca taagttttgt tgcatctcct tgttttgaaa cagaattttt cttaatactc
     5401 gaaacaaagt agctttttca tgacctctcg gtaaacggtg ttcaatcagc tgcttctcgg
     5461 taataagctc actagcctag aaaatatgag aagctatttt ctgactagcg ctcttattac
     5521 tcaaagcata acgcagtttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct
     5581 gcggtaataa gcccaaccca acaaaaaata tgagtagcta tttttttggg ctgtacttat
     5641 tacttgatgc aggacagctt tttcacaacc tcatcggctt tcattttcta tttttttcct
     5701 gaacccatat aataaaagaa agaacaaaaa aacttgcaag ggaggacaca tgtgaagcag
     5761 agtaagcaaa aacagtctcg tcgacttttg tttcgtttgt ttttattaat atgtatcgtc
     5821 gtagggttac tgatcgcttt atatccattt tatgtggatt ctttaaatag ttttattgat
     5881 caaaaacgta tcgaacaagt acaaaaacgg accgcagctg aaattgaagt gcagcgaaag
     5941 aaaatggagg agaaaaacca acgattaact gaccaaggat tcaatcctgg tactgatcct
     6001 ttcaatgaaa agaacagaaa tgaaagtaca acatccagcc aattagagga atggctgatc
     6061 ggaagtatca atatacctaa gatccaatta aatatttcac tttatgatcg gctcaatggt
     6121 aggatccttg aaaatggagc aggcgtcttg caaggaacct ctttcccgct tggtggaaat
     6181 tcgacgcatt cagtgatctc tgctcacagc ggtttaccta atcgacggtt atttaccgaa
     6241 ttagatcgtt tagaaaacgg agacacgttt attttgactg ttttggggga gaaattggct
     6301 tatcaagtta aaaatataca agtggtactg ccagatgaca cgtctgttct aacgatttca
     6361 gaaggagaag acttagttac cttgcttaca tgtacgccgt atatgatcaa cacgcatcgt
     6421 ttgctagtga caggacatcg gattccttat aacgaatcgg taaaaaaaga agaaggaaaa
     6481 ggaaatcagg aaagaaccat gcggcaatgg tttattcttg caggaacgat aatagctgta
     6541 gtcatactgt tagtgtttat cggacgacta atctatcagt atagattaag taaaaaagtg
     6601 cttgattttt catttattat tagtgattcg tcagggaaac cggtaaatgg tagttcattc
     6661 attttgaaac ataagaaaaa aacacttact agaaatggtg tgccgttttc agtacaaagc
     6721 gaccatcatg ggaaagtcaa gctggatcaa ttgcctggtg gaacttatcg aatcgtttca
     6781 gctgatgatc ctaaagttgc tgtttctttt ggtattagaa aagtaaaaca agagaagata
     6841 tacttttttg aggggcgaaa acttgtaaaa gagcttcaaa aaaatggttt ttattttaaa
     6901 atcaatgaat aagtaaggag agaaaaaatg atccgcacaa caacaaaatt gtctgccaaa
     6961 gaactggaat ctgttctatc gatttggctg catgctaact tggattctca tgaattcatt
     7021 ccatcaactt attgggagaa tcaagtagaa tctgttagga aagctttacc agatgcagtg
     7081 atttacttag cagaaataga tcaagaaatt attggttttg ctggggtgat tgatcagttt
     7141 atcgcaggaa tctttgtgaa aagggaatac cggcaacaaa gaatcggtac gcaactaatg
     7201 aaaaaaataa aagaaaccaa taaaacactg actttgacag tctatcaaaa aaatcaatca
     7261 gctattcatt tctatctttc acaaggtttt acaattcaaa cacagcaaaa agatgaagca
     7321 gtaaatgagg aggaacttgt gatgaaatgg aatcaaaacg ctgatttttg atgaacagaa
     7381 taacgaaata atgctcccag cctaattttt tgctgggagc attatttcat tttgaagtca
     7441 ctaatttaac tgtgtgaata tgcagttgtt acaatctttg ataaacggtg ttcaatcagc
     7501 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttt ccatttgttt
     7561 ctctaatact caggtcaaaa cgctgatttc acaacctctt gataaacggt gttcaaacgg
     7621 ctgcttctcg gtaataagcc cactagccta gaaaatatga aaagctattt tctaactagc
     7681 gttcttatta ctcaaagcat aacgctgatt tcacaacctc ttgataaacg gtgttcaatc
     7741 agctgaccct cggtattagc tcaaaaaacg gcaaaacatg aggaactatt tttccgtttc
     7801 tttctctaat actcaggtca gaacgctgat ttcacaacct ctctttcaca accttatttt
     7861 actcgtttat aattttcatc gaagtatttt ccgctacgga tatcatctgg cattccatcg
     7921 tatggttctt cgctaataac atcttcattg tctatacctg catcacccat ataagtgatc
     7981 gtagcaaatt caggaacaag taattttgga taagtttcat atttttcacg agctgcttcc
     8041 atttctgcga tatgttcgtt ttggaaagta acagtgattt ctggatgttc ttcaacccat
     8101 ttagggaaaa agatcattcc gtgaagtttc ttttcatttg gcataaagtc caatgccacg
     8161 tctgtccctg ttggctctgt ccaagtgatt ttgaatacgc cgtccgtcaa tttaacgata
     8221 tcagcttctt ggtcttttac ccaacggcct ttgaccattc cgccatggat acggtaatca
     8281 acagtatggt catttttggc ataccattca tattcccaac cattgtcata cgtatagata
     8341 aagtgagttc caataaaatc ttccaattct ttaaattctt tcatgtttaa gcatctcctt
     8401 ataaattaaa acatgtaata aactacatgt tttcgattac tcatttatga tagaatggat
     8461 ttaaagattt gtcaaggagg gaaaactgat ggcaagaaaa aatacacttc gatatatttt
     8521 gttagggtta ttaagtaaga agaagatgac aggatatgaa ttgaatcaat cgttcaaaaa
     8581 tgaaatagga gaattttggc aagcaaaaca tagtcagatt tatcctgaac ttgcaaaaat
     8641 ggaagagcaa ggaatcattc agcatcaagt agagatcaca ggtgaaaaat tggaaaagaa
     8701 agtttatgaa ttaacagaag aagggaagga acaattggca gagtggattc atacacccac
     8761 caatgaacta ccggttaatc gagacgaatt tgttctgaaa ttgtatttcg tcaaagacgt
     8821 gaatgatccg gcattagtag aaattattca gcagcagcgt gaccttcatg aagaaaaacg
     8881 acttcatcta ttagagcgaa aaaaaacgat ctttcctact gaaaaagaaa aacatgaaaa
     8941 ttacggacac tacctgattt tagcgcatgc gatcaatcgg gaaacagaat atgcaaattg
     9001 gctaacagat gtattagaag aaagtaaccg aaaaaaataa aaaaatatcc gaacagtgat
     9061 ggagaattcc tttctatatg aaagaaacgc agatcaccgt tcggatattc ttatgttcaa
     9121 acaggccttt tttcaagtat ttatgtagca tctacttatt aaaaagcttg gtacttagga
     9181 gattagtggc gtttagagtt cttttaacaa ctgttcgtag tcagcttttt catcgacagg
     9241 aaggagcttg taagttaatt cgtctgtctc gttgtcatga atgatgtaca tcttagcatt
     9301 ctcttctttg aagttaccga tttgtaaaat cgtatagcgt tcgtcgatca attcgatatc
     9361 tttgattgct tttgaaaaaa ctttacggac atggtgcatt ttcttttggc ggatttctgc
     9421 cttttctgca gcttttgaca aagtaaatcc gtcatctaaa tattttttga tcagttctac
     9481 ttttatgact gtaggtaaac gatattttct aggacaattc gcatcagctt cgatcgaacg
     9541 aatgaatccc ttttgttccc aataccttaa ttgacgtgga gacacaccta cgatatcact
     9601 taattcactg atgccaacaa ccaattgatc attttccaac aaacgtttaa gggaagcccc
     9661 aaccattttt gtcactcctt ttgtcctctt gcttccctta attatatccg tattaactta
     9721 tgtgtcaagt aggttgacaa atagcgaatt taggactaaa gtattctagt agtttcagaa
     9781 aaaattcaga aagaaggaga aatatgagta acaaccaacc cgtagatatt tacggaaaac
     9841 catataatcg gacactttta gttgttgtct tattaattgg tacattttgt accgttttga
     9901 atcagacctt attgacgaca gcttttccta cattgatgaa agctttcgat atttcagcgt
     9961 ctgatgtgca atggttaacg actggtttct tgctagtcaa cgggatcatg atcccaataa
    10021 cagcatggct gatcaataaa ttcagttcaa gaaatttata tctttctgcc atgacgatct
    10081 ttttgatcgg aacgattact tgctttacag cacctaattt tagtacatta ctaatcggtc
    10141 gtttgatcca agcttgcggt gtgggagtat cgatgccatt attgcaaaat atcatgctat
    10201 ctattttccc gccagaaaaa agaggatcag caatggggat gtcagggatc gttattggac
    10261 ttgctcctgc tttaggccct acattatctg gttatatcat tgataactac cattggcgtg
    10321 acttgtttgg tatggtactg ccaatcgttg ttcttgtctt ggttttagca ttcttcctaa
    10381 tgaaaagtgt tattccattg tcaaatccta gcattgatat tttatccgct attttatcaa
    10441 cgattggatt cggtagttta ctttacggat tttctagtgt aggagatgac ggctggggaa
    10501 gcttgaaagt aattggtttc ttagttgttg gtgtgatttt catcgccttg ttctcatggc
    10561 gtcaattaca tttggaacat ccattcttgg aacttcgtgt attcaaatca tctacattca
    10621 cgattgcagc tgttttagct ggtgtaacaa atatggcgat ggtaggggca gaaatggtcg
    10681 taccattgta tattcaaaat attcgtggag aatcagcatt ccattctggc ttgatgttgt
    10741 tgccgggtgc attgatcatg ggggctatga tgcctgtgac gggtgcgatt ttcgataaat
    10801 acggcgcgaa acgtttagcc atcacaggga tgttcatttt gacagcggca actgcaccat
    10861 ttgctttctt gacaaaaacg actccagttt tatatatcgt gatcctttat gcgatccgta
    10921 tgtttgggat ctccatggtc atgatgccgg taacgacttc tggtatgaat gcattgccaa
    10981 ataacttgat cagtcatggg acagcggtaa ataatacatt ccgtcaaatc gcaagctcga
    11041 ttggtacagc tattttgatc agtgtgttga cgaatgtgac aaaagcaaat atcccaagca
    11101 aatctgtatt ggataatacg ccattagctt ataaagatca ggcaatcaat gccacactgt
    11161 caggttacca tgccgcgttt atcgtagcga tcatctttgg gattcttggc tttattattg
    11221 cctttttatt gaagaaaaaa gaacctgttc taatgaaagc gggggataat gcatgattat
    11281 tggagttttg attgtcagct tgcttgcttt tgctttaacg aacgtgttcg ctaaaaaaac
    11341 atggcaaacc tttttatctt tgattttcgg actaatcttc gtcgcttcac taagtttgat
    11401 cgtcgctaat ttgtccaatc actttggaat ggaaaaagta acggaaacaa aaacagaaaa
    11461 aattgtttcc agtgctgatt cccaaggagc agatatgctt ttgtataaag ctttagggaa
    11521 tggaaaagaa aaagtgtatc tttaccgaac aaatgaaaaa caagaaaaac caaaagcaac
    11581 tggtacggat aacgaaacaa ataaagtaga aaagacagac ggcgatgctg aaaaagtgac
    11641 gaaaactact tattgggaat acaaaaacga catgtataaa ttctggttca atattgctga
    11701 taacaatcac gaatatgaca gccgagtgaa tacattcaaa atcccagaga catgggtaga
    11761 attaagtacg gaccaagcag ccaagttagc tgaattagtg aagaaacagc aaagtacgat
    11821 ggaaagtgaa gcaaaagctt atgttcaaga cggtatggta aaagcaatga ctgaaaatcc
    11881 aaaaatgagc aaagcagagc aagaacaacg tacgaaagcc ttagctgctg aattccaaca
    11941 acaagcattc gcaaaattag tgaaagaagc aaaaggcgaa taatattttg aacaccatgt
    12001 aggaggttgt gaaaaagctg ctctgcatca agcagtaaga acaatcagat aaaaatagca
    12061 ctccatattt ttcatctgat tgggcttagt gccgtagatg caagcttttg aacaccgttt
    12121 aaggaagagg ttgtgagcct gccgtttaac tctgagtcac aaggagcaat ttcaaaaaac
    12181 agcttctcat gtttgttgac tattgtgact tgtgaccgag gagttggcag gagaacaccg
    12241 tttattcgaa aaaaggaact gcgacaagac ttttgtcaca gttccttctt ttttttgcta
    12301 tgctatacta gatgtaaaaa ataggagaaa taaaatgagg gaaatttatt cttttgaatc
    12361 aaaaatctat gcttccgaag tcggcaaggg cggggcatat attatttttc cgtatgatat
    12421 aagaaaagaa ttcggtgctg gaagagttaa agtccaagca acttttgaca atgtgccgta
    12481 tgaaggcagt atcgtgaata tgggcgtgaa aaatccagat ggttcagtct gctacatcct
    12541 tggactacga aaagatattc gtaaaaacat cggaaaagaa atcggtgata ttgtagcagt
    12601 tacagtgaag caaaaataaa agaggttgtg acaaaagacg tgtcacaacc tcttgataaa
    12661 cggtgttcaa aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaaccc
    12721 atgtttacaa aatttttctt actactcgaa acgaagcggc tttatcacaa cctcttgata
    12781 aacggcgttc aatcagctgt ccctcggtat tagctcaaaa acggcaaaat atgagaagct
    12841 atttttccat ttttttctct catactcagg tcataacgct gatttcacaa cctcttcttt
    12901 cataatggtt tttcaccatc tttataataa actggttatt tgatatggaa atctattttt
    12961 tccaaccaat ctaaaatctt gtaacctaac tcttctgaca tcggaaaaga ggcgttgatt
    13021 tgtgcattgg agatatggtc aacatggata ccggaagtga tcgtacaact tcccggaacg
    13081 gcagttttga tgatcacagc tattttttcg gctaagacat cgtctttatg gaaccgacca
    13141 tcgtggctag ggaaacgtac agtttgcatc tctgtatcac gagaaaatgt agtgactgtt
    13201 ccgatatgtg gtgtatctcc gcctgttaaa gtgatcaata agtctttacc gattacttta
    13261 acagcagctt ggatcgtgaa acctagtttg gttacttcaa ctatcgtttc tttttccata
    13321 ttaatcaccc tgccaacgtt ttgaatgctc atttacaatt tcaaattgat ccaaaagttt
    13381 cattgtatgg tgattaacaa gatcttggat cgtttgaggc tgattataaa aagcaggaat
    13441 tggtggtatg atttgcacac ctagtcgtgc taatttagtc aagttttcca agtgaatggc
    13501 atttaatggc gtttcacgag gaacaatgac taactttcgt tgctccttca ttgttacatc
    13561 agcagcacgt gcaatcaggt tgtcgcctaa accaacagag atacctgcga ctgttttcat
    13621 acttgccggt acgatgatca ttccatcggt caaaaatgat ccgctggcga cagcagcacc
    13681 taaatctttg tttgaataat ggtaatcaaa caggctttcg aattcagtta gtgataagtc
    13741 ggattccagc tttaagttct gttttgccca tggactgatg attccgtgtg tttctatcat
    13801 cggatattct tttagtttct tcactaggtt gatggcataa atggtgcctg aagcgccact
    13861 tacaccaatc acgatttttt tcttccgttg tgccaattgt tccattttcc gcactccttt
    13921 ttatgatgtc aaaacagttg cagatcattc attatttcaa atatttttcc caatcttcta
    13981 cttcttggaa ttttgcacgt acaaaatgtt ctttcatatc gaaaggaact gtaccgtcaa
    14041 agatagtttt tgcacttatc ccacggaaac gaatagagcc aggattatat tctggtaatt
    14101 ctgatgggtc caatggatgg ttcctcattc ctgggatcac gatgatatct ttatctcctt
    14161 ggaaacgagt gttgaaagtc caaactacat cgttcatgtc gaaaatatca acatcttcat
    14221 cgactaaaat gactgtcttc aattctttga aggctgataa agcaagaata gctgcttgac
    14281 gctgaatacc ttcatccgct tcgttttctt tacggatttg catgatggtc atcaatttac
    14341 caccacctgc aggaggattg tagacattta ctacttttcc tggaatagct ttatcgacca
    14401 aggcaagaat acttgcttct gtaggaatac cggccataga tacgtgttct tcactgggac
    14461 caatcgttgt ttgcatgatt ggattgtctt ttcggtgagt aactgctttg actttcacta
    14521 cttgtacagc tggattagca tccccagtat agccaggaaa ttctggcatt gcttttcctg
    14581 agttcgtgtt gatatcttct tgcattgttt catttggcat gatttctgct tcaatgatga
    14641 attcagcacg agcaataccc acttcatcga ctgctatacc atcaacgatt tgaataggtt
    14701 cgttacgcaa agctcctgca caccatagtt cattgtagcc aagtggagtg gtaggtggtt
    14761 cgaaagtcgt gccaatggca attgctggat ctaaaccaat attgatagtg ataggcattg
    14821 gtttattcaa ggcttcgaat tgtttttgga agtgaccaat gtggcgtccg ccaggcatga
    14881 tgtacatacc gatcgtatct ttatcttcta gaaccatacg atggatcgtt acatcgctca
    14941 ttgttttttc tggatctgat cctaagacta cacccattgt gatgtatgga ccagcatctt
    15001 caggagtatt tgtaggtgca ggcaaaatgt tgcgaatatc gaagtctgca tcggtagcta
    15061 gatgaacgac ttcttgtgct ggagcgtctg ctttgtctac tttcacaggt ttgattggat
    15121 tttctacagc gtctttcaag aaacgaccaa gtgttcgata atcatggtgc aaaatttcac
    15181 ctacacgttt acgactagcc attgttccga tgttcacacg tgtgccgggg aaacctttga
    15241 tattgttgaa agtcatagct ggtccttctt gagtcggacg ttttactgta ccgccagcac
    15301 cgatataacg gtaaacacca gataattctg cttcaggatc gatttcaata tctgtttcgt
    15361 gatattgacc tggaagtgtt tttagttctt cgatgacttt tcttaaatca tacggttgat
    15421 ctgtcatgag agattcctcc ttagttgtct ttacctatct atagtaaaag gtgaaaaaga
    15481 ggaaaacaat tcaacttgtt cggtaaagta ttctacaatg gaatggatgc gtttttttga
    15541 taatagaata aactatgata tacttatcac taaaagaaag cgcatactta gtgttggagg
    15601 aaggaaagtg aaaaaaatgg aggagtcatt tcctaaagca gtaaaagtgg aaaatatagc
    15661 gaatattttg aaagttacat ttgaaaacgg agaagtaaaa tacgtgaaat ctcattggac
    15721 agaagagata accgatgcct tgcaatttgg aaaaaaagga agaggaaaaa gaaaaaatct
    15781 tctcgcactt tctcgtaata tgtggattgg gactgaagta acgattgaag cagatggaac
    15841 agtattcata aatggaaaag acaggtatac gccagaagag ttgtggtaca aaggaaagaa
    15901 aagtatacca gaattataaa aaataattaa aaaaaacagc gcgaaacaag gaacatatta
    15961 cttcctcgtt tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt
    16021 ggttgtcaca accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc
    16081 gtgcaggaag atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg
    16141 catggacagg atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat
    16201 tgatagggaa cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta
    16261 aaatacacgt ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt
    16321 ttagtacacc gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca
    16381 tgatcatact tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat
    16441 ccatcaatcc gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat
    16501 cttcatctga tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt
    16561 caaaattgtt cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa
    16621 gcaatgtttg tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt
    16681 ctctttgagc tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt
    16741 gatcgctata agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta
    16801 ggatccccga gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat
    16861 tagggataaa attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga
    16921 atgttgtacc aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa
    16981 gattcttcaa tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg
    17041 ttgctgaggc tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt
    17101 tgctagtgtc aagctgtgta gggaaacttg acactcccac acctgtcatt gctgacatcg
    17161 tactagataa agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta
    17221 ctttgccatt tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat
    17281 tacttaagtt aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg
    17341 tgtgctgtgc tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat
    17401 ccgctttgct tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa
    17461 tagtaatcgg aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc
    17521 cagaagaaag ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg
    17581 tcaagaaagt acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc
    17641 aaaaactcat ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag
    17701 gatgggaatc tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat
    17761 gggccagttc aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga
    17821 tcaattccat gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca
    17881 aaaggatatc tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac
    17941 cagataactg atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat
    18001 cttctgcccg ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa
    18061 cgttttcaat aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag
    18121 agttgtttcg gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga
    18181 ttaccatatc accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt
    18241 taccagaacc actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca
    18301 caccatccaa tgctttggtg gtggtttcgc cgactttata atattttttt atatctttta
    18361 attgaagcat aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca
    18421 gaacaatgat cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc
    18481 tagaaatttt tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga
    18541 tccgcaatta cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac
    18601 cgcattgaat ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat
    18661 tacagatatc atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac
    18721 cagatttgac acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg
    18781 cgggttcaaa ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat
    18841 cctacgtgtg gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat
    18901 cggacaaaca aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat
    18961 tctgcctcaa aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt
    19021 cactcgccaa cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac
    19081 gatcgatgta catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat
    19141 catcacagta cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt
    19201 tcacaattat ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg
    19261 tgttttctcg gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct
    19321 ggaaaaccaa cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca
    19381 ggaatatcga tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat
    19441 atgagtctgg tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca
    19501 gaagtacagc aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag
    19561 acactgagga atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca
    19621 atccaaggat atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata
    19681 tttttatatc ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta
    19741 aaattgaaca aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat
    19801 gaacaaatca gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa
    19861 ttggatatcg aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg
    19921 tggctgaata tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc
    19981 aaactatttg agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg
    20041 aatgaagaga ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa
    20101 atttctggga atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga
    20161 cgtattgatt atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa
    20221 caataggaaa aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt
    20281 cgtttttttg tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt
    20341 cttattgaac tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag
    20401 agagcgaaag tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg
    20461 gcttagtttt cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca
    20521 ccgactttac gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca
    20581 gtattttctt ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca
    20641 tttgcaatca tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca
    20701 aatccagctt cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg
    20761 agcaaaaagt tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga
    20821 tgatttttag gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa
    20881 cttggtgggg cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca
    20941 ttgtaagtta attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt
    21001 ttcaaaaact cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag
    21061 atatttaatg aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg
    21121 tattgttcta cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg
    21181 atttttctat gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc
    21241 tgtttagaaa tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt
    21301 gtttcggcta aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt
    21361 attccaataa agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta
    21421 caatctttct tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt
    21481 agaaaaagag aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt
    21541 gtttggcgga ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca
    21601 gctgacgatg gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat
    21661 gaaaaatatg ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt
    21721 tcatttaggt aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat
    21781 cttccctagt gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt
    21841 cattgttcta ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac
    21901 aggagacagt acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt
    21961 tgcgtgtaat ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta
    22021 tattttgttc ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga
    22081 ttttaaagct tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa
    22141 aatgttccca gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat
    22201 gtggacaaat tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct
    22261 gactaaaaag gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa
    22321 ctatattata tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt
    22381 gacgtaatgc acatccagat tccaactgtt cttcttgatg gtactgtgct agcagcatta
    22441 agcaaggaag atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc
    22501 agtgaatcta cactatatcc ggttttgaga agattgaaaa aagaaggata tcttgagacc
    22561 tatgatgaac cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt
    22621 cgtcatttag aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta
    22681 ttggaggaga gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat
    22741 tcctgaagac gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg
    22801 aaaaacatta gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt
    22861 aaaaatcagt tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa
    22921 aagacaaatt cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat
    22981 tccactagca ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct
    23041 catttttagt atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat
    23101 ctcaggaatc agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg
    23161 tctgttcgcc acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg
    23221 gcttttttat gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag
    23281 agatctgcat tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt
    23341 gattaccggt attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt
    23401 aggcgctcaa aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga
    23461 gacaaaaaaa atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc
    23521 ggtcatacgc ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga
    23581 aataaaggag aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat
    23641 cattagttta gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt
    23701 atctgaattg acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa
    23761 aaaagtgaat actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac
    23821 aataactgcg aaagatgaat taggctcact tcgattagat gaggccaatg tggataactt
    23881 gatcgtcaca ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg
    23941 aaaagtcatc ctaaacggtg gcaatttatc tttgcatgag atggaaaaca gtggctatac
    24001 tatccaaacg tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt
    24061 gttagaacaa ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac
    24121 gacggatcaa gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa
    24181 aaacagcaaa gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag
    24241 taaaataaga aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa
    24301 gaaggtcaaa gagaaaagtt aaaaacagaa atggaaagat tacatactta tatcactcaa
    24361 ctaagccaaa ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat
    24421 tcagaaggtc gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg
    24481 aagaaagagc tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat
    24541 gggaagaagg aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg
    24601 atggatctag aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca
    24661 aaattggatt atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact
    24721 gaagacaaag agttagaact agaaggtgta atggcgcgta ttcgaaaaaa aaattaaaaa
    24781 agtaacatta gatatttatt gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa
    24841 gagcatttta tatcaatggt tatgcaggga ttgtcggatt ggtcgtatta gctttagttg
    24901 gattgttttt cttctattta ggtatgtggc aagcgaaagt ttgggcactt tttttaagta
    24961 tttttctttg gttgatcacg ttattgttgt taagttcggc aactgtagtc agtcctaatc
    25021 aagcaaaagt aattttgttt tttggacaat atttaggtac gattcgagaa aatggctttt
    25081 tccttacgat acctttggca caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata
    25141 gttctgtttt aaaagtcaat gatttggatg gaaaccccat cgaaatctca gcagtagtag
    25201 tctttaaagt cgttgataca gcaaaggcat tattcgatgt agcgtattat caagattttg
    25261 tagaaatcca aagtgagaca gccattcggc atatcgctag ccaatacccg tatgatacat
    25321 tcaatgagaa tgatttgacg cttcgaggaa acacgacggc agtgtctgac gagttgcaaa
    25381 aagagctgca agagcgttta gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc
    25441 atttagccta tgcaacagaa atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca
    25501 ttttgtctgc tcgacaaacc attgttgaag gagctgtcac aatcactcaa atggccttag
    25561 agcaaatcga agatggacaa gaaatcaatt tcaccgatga tcggaaagtc caattgatca
    25621 ataatttact tgtctcaatc attacagaca aaggaacgca acctgttatc aatacaggcg
    25681 acatgaaaga ccgtaagtga catacttttt gtaagatagg aagaaggagt ttaggacgga
    25741 ttattgtctt gaactctttt tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa
    25801 tgatagttga ttcgaaggca gaattgttcg cttttttcta taatcagtat ataaagtaag
    25861 agagaagggg aaaaagtgaa aaaacttctg atcggtctac ttgttttcat cgttgtggta
    25921 cttgcagggg tgaaaatcgc agattacgta gttatgggtg gagaagaata ctatgtacaa
    25981 attacgacag atggtacaaa aacagtttca aaatcagact ctggacaaga aacagtcaac
    26041 tatagatata tcctgcctgg ctatacgaaa gagggtaagg aaaaagaact ggacttcaag
    26101 ggacaattga ctcgaccgct tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat
    26161 aaaggtgtca cttcgtatga agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa
    26221 aaactaatta aagggtaggg aaagaaatga acaaaatagt cgaagtacag aatgtaacaa
    26281 aagtatacgg aaaaaacaat gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg
    26341 tagaaaaagg cgaatttatt gggatcatgg gcgcttctgg ttctggtaag tcgacacttt
    26401 tgaatatttt atccacatta gataaaccaa cagatgggca tatccgaatc aatcaaaaag
    26461 atgtgaccac attaaaagga aatcaactag cagattttcg tgcgaatgag attggcttta
    26521 ttttccaaga tttcaattta ttagaaagct tgacagcaca agaaaatatc gctgttcctc
    26581 tttctttaca aggcgtcaga ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac
    26641 gtttatccat ctctcatatt ttggaaagct atccttctga gatttccggt ggacaaaaac
    26701 aacgggtcgc cgccgcacgt gcattgatca cacaaccaac gattctatta ggggatgagc
    26761 ctacaggtgc gctggattcc aaaagtgccc gggatctttt agatacgatg gacgaattga
    26821 atacaaaaga tcatgtatcg attcttcttg tgacgcatga tccgttttct gcaagttatt
    26881 gccaacgtat tttattcatc aaggatggcg gtatccatca agaagtgaaa cgtggagatc
    26941 aaagccgtga ttccttctat cgagaaatac tatctattct tggaaacttg gaacagtagg
    27001 gggagattga ctatgctttg gaaattatca cttactggga tcaagagccg tttaagagac
    27061 tacttggtgt tattctcagg attagttatg gcatctgcta ttttttacat gtttgaatca
    27121 atggcaagca acgaagcttt tttgaaaagt aatacgatcg ttgcggctac agttatcatt
    27181 ttccgattcg gttctgtttt actagggatc atcacattcg tttatatttt atatgcaaat
    27241 tcctttttga tgacgatgcg gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa
    27301 ggcagaaaaa tcgcacagat gattttttct gaaacattct tagtagggat cgcagctact
    27361 ttagtgggtt cagctgtcgg tgtaggattg acaagtatcg tccaccgatt attagtggat
    27421 cagttagatc tcaatatttc gcattttaca ccttttagta ttcacgggtt gttgatcact
    27481 gttttgtttt ttgctgtttt gtttttatta gctgctgttg tcaatgcttt ttcgattgtc
    27541 aaaaaaccga ttttagcatt gattcgtgcg gatcagacac caacaagaat caagcagcat
    27601 aaattcttgt ttttccttga agtagtactg ggaattgctt ttctaggctg cggttactat
    27661 atgatgaaac acgtggggac ctatcaagtc tttggtatag gggtagcctt agttacgatc
    27721 gtattaggaa cttatttcat tttccattct gtgatcattt tctttttatc attactaaag
    27781 aaatcagaac agatttcatt gaaaaaattg aacaatttca cattgtcaca gttaaacttt
    27841 cgtattcgtg aatatacaca aatgctctca atggtcgcta tgctgtttgc cttggcactg
    27901 ggcgctttga cggtaggatt aggttttaaa aatgaaattg cagacttgac agaaaacaat
    27961 tctggatatg atctagtgat gaataatcct cgggcagaag atcagcaaaa aatcaaagaa
    28021 ctttcaccaa cactttctgc ttcttatact caaaaagaag atgcgggaac gatttactat
    28081 aatgcaagtg aatttgcggc tcaaccattg atcatgataa aacatgaaga tggaacaccg
    28141 ccgaagatca cgaaagaaaa agttcctgct aaaaaaatga atgaactgac tgtacaagag
    28201 gaacttcgaa gctatgaatt gccggaacag caagaaaaag aaatcaaggt agtcagtcaa
    28261 tcagaatttg atcaactgaa cttgccacaa tccactttac agctagttca ggtaaaagac
    28321 ttccaagcaa acctgaaacc aatcgaaaca cttgttgctc aaaataaaat gaacaatcca
    28381 tctttgcaaa aagtagagta cagcaaccag aaatatgaaa tgtatgaagt gtataatggt
    28441 ctattctctg gttttgaatt tatgggattc ttcctaggtt tagctttctt gaccatgctt
    28501 gctagctgtt tgatgttcaa gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa
    28561 atgcttgaaa agataggaac acgacgcggg ttgcttaaac aatcgatccg tagagaagta
    28621 ggcgtgttat tcttagcacc aggaatctta ggtgtagtcc atgtcttatt cggattacaa
    28681 atgtttggtc tattcatgca aaatccatac aaagatattt gggttccgtt tacgatattc
    28741 ttcgtattgt atttcatcta ctatgtactg acgacatggt tatatactgg aatcgtattg
    28801 aaaaagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata
    28861 gctcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    28921 tttgttcgga aaaagaggtt gtgacaatat ttttgtcaca accttttttt cacggtcttt
    28981 tattagaata tagtaaattt tttctcgtta ctcacggaat aagctgttaa gaaaacggtt
    29041 tccatgtata ctggaatcag tgcaaatgaa agaaaagagg gtgcaaggaa catggaaatc
    29101 aatgaacaaa attttggcga aaactatcac ttgattacga tcacgaatca aaatgggaca
    29161 tctttgtctg tcagtgatct tggggcacgg gttgttcatt ttcaaacaat aatcgataat
    29221 gaaaaacgag agttgatctt aggttttgat gaagcaaaag aatatctaga aaaagaccct
    29281 tatatcggtg cttcaatcgg acgtatggct ggacggatta cgaatggcac attcaaatta
    29341 gatgatgaaa cgttcgaagt aaaaacagac ccggacactg gtcattgttt acatggtggt
    29401 gcacctagct ttgaagccaa gcagtggcat tatacagtca tcaatggtga aaacgaagca
    29461 tcggtcattt tctatcagac tagcccagat atggaaaacg gatttcctgg aactttagat
    29521 gtagaagtac ggtatacgct gacgaacgat gatacatggc gagtgacggt ccaagggatc
    29581 agtgaccgca aaacagtatt caatcctaca aatcatgtct atttcaattt aaccggtgat
    29641 ccagcacagt caatcgacga acatatactt tggttgaata gccgattatt tgctacgctc
    29701 aataaagata caacgcctac aggagaagta gtttcagtaa gtggcacagc atttgacttc
    29761 caacgtccta aaaaattagg tgaggtcttt gccagtgatt ttcgccaatc aatgatagtg
    29821 ggcggcatcg atcatccatt tttgttgaaa aaacaatctg gtttactaaa tgctgcggaa
    29881 ttgacaagcc cagatgaaaa agtatcgatc caagtaaaaa cagatgcttc aggaatcgtg
    29941 atttttacag caaattttgg agaagatgga ccggaaatgc gcgggaataa attagtaaat
    30001 catggcggta tcacatttga gactcaagaa ctgccaggag cggagcgact tgaaagtttt
    30061 ggagatgtca ctctggaacc taaccaagta cgcgagtttg taacggaata caaaataaat
    30121 ataaagaaat agaataaatt aaaaagggag tgtgacagtg tcacatcccc tttttaattt
    30181 attctatttc tttaaaggca gatcaagcca gaatgatttg tctaacctta acaaggttag
    30241 tacaggaaca gttgcagctt taactgtacg agtagttgtt tctggtatct gtcttgccgt
    30301 aaaattgttc cacaaatccc agattgaagc gacacggaag atggaagaag cgttgttgac
    30361 gcgttttatc gttgaaaatt ctaatcctgt gattggcacg ccatctgttg tccatttgat
    30421 tggcacatcg aatcgcttag acatattgag gatttcagcg tttgcttctt cagttgttgc
    30481 tgcttctttt ttcggttcat catattttgt caggtcgtac gcttcgatga tcgcgttcaa
    30541 tttttgtgaa tattggcgat ctgtagcata acgtccttcc aaatatttgg ttgcttcttt
    30601 gtagtctttt gtttccgact tccaagttgg tttgtagaaa tcaggattgc cagtcagtcc
    30661 atttttgatc aagtcggcat agtcttccaa actttccttg taagaaggat atttacggaa
    30721 ttgtgcgcgg atttggtagc tttgtccgga actatcttgt tcaagtgtat tgaaggtcac
    30781 gctgcttcct ttatatgatc ctttgatacc aaaaaggtta tgatttggtt cgcttgataa
    30841 taagctgttt ccagatccac tttcaaggat agcttgagcc atcatgactg atgcgtagat
    30901 tccttctttt tgcccgattt cctgtgcatc atctgcaatc tttttcaaga actgatcagt
    30961 tgtttggttt tgactgaaat ggaacgagcc atctaatcca tctaaaatag atgagccgcc
    31021 tgtcagcgaa cctgaagcag caggtaaacc tgctaagttt actcggacag caaaatcagg
    31081 cgtgaaatct gaaatagaag ttactcgaac tttatctcct ggttgaggcg catggataaa
    31141 ttgtccgttc ccaatataga tacctacatg gtgagtaaca ccggaagaag gtccccagaa
    31201 gacgaggtcg ccaggttggg cctgacttac tggaatctgc gtacctgcat attgctgttc
    31261 gcctgtccag ccaccgatat aatgaccggt tgctttcata tatacgtaat aagtcaaacc
    31321 agaacagtca aaagtatttg gaccttttgc tccccataca tatggttttc caagatgctt
    31381 gaatgcttct cgaacgattg cttgttggac attcgttgta tttgtaggca atacagcatc
    31441 aattggtgat tttacaacaa aatcagaaga agcagaagat ggattctcta taccgctttg
    31501 atttgctgta tttggacgag atgaagcatt gttcgttccg ccattcgatg aaccattgtt
    31561 tggatggttt ggttctctct gagtatttcc attgttattc gatccagatg ggtttgtttg
    31621 attcgaagaa ttcccgctgt tcgaattatt gttattattg tttgaattat ttgaaccatt
    31681 attattgttg ttattaccca catctggttt gtttggcgtt tccggatctg gtgtactagg
    31741 gacttcttca ctaggatcag ttggtttctc tgttgaagta tccggtccag ctttaaattc
    31801 tgcagtaata gataaattgt tcgtacctac tgtaaaggtc tttgcatcat aatttacttc
    31861 tttggcacca ttaacagcga ctgaagcaat ttgttcacct tcagctggcg taatagtgta
    31921 agttaccttc gtttccgcta ataaaccttc taccgttcca ctttccgtaa tatccaaagg
    31981 ttgctgttca ggggaatccg ctgtcgaagc agtcagttgg atcttaccaa tcaaagaagg
    32041 agcaattgta agggaatatg tctcggctgg cttttcattt tctgatggat cagccggatc
    32101 aatttcagtt ggctcggtcg atcctgttgt tgagttagtt gttccagttg ggtcttctgt
    32161 aaaagaatcc gttgtttcac tagtagctga gctggatgta gtcgtatctg ttgtgactgt
    32221 ttgatcagct atttgattat ctacggcaaa tacggtattg gtcgacaaaa cagacggagc
    32281 aatcagccaa gtagcagcta gcaaacgtat cgttttttta tttttcataa taaactagtc
    32341 cttttccttt cattgatttt ctgtcttatt cagcattcat tagacatttt ttggaacaga
    32401 taatcgtgtc gaaaagcttt ttcttttcat aaatctgtct gtgtattcta aataaaacaa
    32461 tttgtaaata agcaacgagc attcattcgt ttttctttga cgttcatttt attgcataga
    32521 ccacctttgt ttggggataa acaagtgggg ataaaaatgg ttatgcatat gtaaattata
    32581 ccaaagtttg gaaagtatca caaaaattta taaaaataat gatcctaaaa agaaaaagac
    32641 tgaaatatgt gataaacatc tcatacttca gtctttttca ttaactattc ataagacatt
    32701 aggtcttcat caaacccgct aacgagagta gtgatcattg ctacgactag actaacagcc
    32761 agtcctgcta agtaccagaa aaaagtaccg gaaatgaatg caggcaatgt ggtcagacta
    32821 ccaaatacaa aagcgatcgc tttcacttgg gcaaatccca ttacagctcc accagcagct
    32881 gcaccaatgc aagcagcaac aaatggtttt ttcaatggga tcgcaattcc gtaaacagtt
    32941 ggttcggtca ctcccgcaag taaaatcgaa gcaaaagcag aaccggagat cgaacgggtt
    33001 tttgaacgtt tacttcgcag aaatacacct aaagctgcac cagccattcc aaaattggct
    33061 gctcctgtca acggaacgat tcggtcgaac ccataagcag agatattact gatcatgaca
    33121 gggtttacag cccattggat accaaatatt accaaaatat tccaaccacc agcgatgatt
    33181 gcgccactga tcggtccgtt aatatccatc atccaagtaa ttgcggcagc taaaccttcg
    33241 ccaaggtaaa cacccgcagg cccgaatacc attaacgtca aaggaaccgt gataactaat
    33301 gaaagtaaag gtacaaatac taattgcata ttttcgcgaa tatatttttt gaggaatttt
    33361 tcaacaaaag aataaaagaa aatacctaaa atcgccggca tgacagtaga cgtatactgc
    33421 atcagtacta caggaatccc cagaaaatca gtagctgctt tgccactgtc tgaaagtaaa
    33481 gaggtcagat tcggttcaat cagtgctgca gcaatgacag ccgcaatata tccattcact
    33541 ttaaacgttt tggcagcaga aaaagcaaga aagattggca gaaaataaaa gacggcattc
    33601 gacgcagcag acaaaatatg ataagttccg cttgtttcac taagccagcc aatcgtagtc
    33661 gctaaaacaa ccagaccttt taatatacca gaaccggcaa acagcggaat gatcggcgta
    33721 aagatttccg atatcaaacc aaatagtttt tcgatcggat ttagcttttc tttcggagct
    33781 gaatgatcga tttgacgcgt caattccttg taagcaggag ccaatccctg atccatgatt
    33841 atttctgcat atctatcgag taaaatgaaa tacttaatgg aagggatttg ctgttttaat
    33901 ttttctgcat tggctactgg ctgttttttc aatttgatac gaagccgagt cgcacatgga
    33961 aggacagaat caatattgtc ttttcctccc aactgttcaa tcagcatatg aatctgctcg
    34021 gacagtacca tctgttcagg attcattgtc gtttccatta gaagaccact ccactcacta
    34081 atataatatt ggcgtaaggt gtcatttcgc ctgtacgaat aaatgcatga cagttattca
    34141 actctttttt catttcttca tgtgggataa agctaatcgg tgtatctggt aacaataatt
    34201 tgattttttc caatatttcc gggttcattg tctttatttc ttccgccaat tcaatcgatt
    34261 gtacctcaag ttcttctaaa acgttttcta aaacagataa aaaacttgga agaccgtttg
    34321 agacagccaa atctattttt tcagtaccaa aggggacagg catgcctgca tccccgatac
    34381 ttaatttatc aaagtgcccc atttgtgcga ttactcgtga aatatctgaa ttgattactt
    34441 ttcctttttt catgtttgtt tcttcctctc attgttaatt ttagactgct gattgttcta
    34501 aagcttcgcg gtaagggata gaaggttgcg caccataaga ctgcacagca acagaagagg
    34561 ctagatttcc ataaaggatc gcttctttca agttactaag gtctggctga agtgtgctgc
    34621 ttaatgcgcc aataaatgta tcacctgcgg ctgttgtgtc aacggcatcc actttaaaag
    34681 cagggacgat accgtgttct ttttctgtat gatagaaagc gccggcactg cctagtgtaa
    34741 tgattactgt tccaatacct aattcatgga gtttttcggc tgctgcgacc aaactgttat
    34801 gatctgtcac tctgattcct gtaatgattt ctgtttctgt ttcatttggt ataatgatat
    34861 ctgtcttttc aagtagttca gtaggaatcg tttcacgagc tggtgcagga ttcaggatcg
    34921 ttgtttttcc tgcgtctttt gcaatagaaa aagctaggat cgtcgcatct aagctacttt
    34981 caaactgagc aataataaag tcactgtttg taatcagttg cttgtttttt aggacttctt
    35041 gttcatggaa agcattattt gcccctgcat ggatcaagat actattttct cctgctgcgt
    35101 ctaccataat cattgcttgt ccggttgtgg cgccttggat ttcagctatc ccagatgtat
    35161 cgatgttttc ttttgtcagt agatctaata actgttgtcc ttcgctgtca gccccaacgc
    35221 ctccaataaa agatgtccgt gcgcctgaac gtttggctgc cactgcttgg ttggcacctt
    35281 tacctccgcc agcgtgaaaa atctcatgcg catgcatcgt ttcgcctggc ttgggcatgt
    35341 tggttaaacg aagggtagtg tccatattaa tacttccaat tactgtaacc gtgttcattt
    35401 tacaaacctc caactatctc atttattttg atgggaaaat tattttttaa aacgttttag
    35461 taaaacgttt taatagttcg ataaaaatat agcacacctt gaaagctatt tcaagggtgc
    35521 agttgaaaat cttttttcta atcgtacagg taatcttttt tcttcccatt ctttttctgg
    35581 gaattggatc cgatctaaca ataacttggc agatgtttgt cccaattcga agattggttg
    35641 tgcgatcgtt gtcagtttcg gcttgatata ctcgcacata tctatattat catagccaat
    35701 aatgctgtag tcttcaggga tgctttttcc agcttcctct aatccgcgat ataatccaaa
    35761 agctaattca tcatttaaag caaacacacc agttgcagca gaatcaagaa gttctgctgt
    35821 gatctggtag ccaccttgtt ttgagaactg tgtaggtaag aggatcagtt ggtcgtgagg
    35881 gatttggtaa acatcaagag cagatttaaa cccctcaatc cgagcgtgga cattttccgg
    35941 tggattttca ggatagacaa gggcgatcgt ttgatgtcct agactaagca agtgcatgcc
    36001 cgctaggtaa ccaccgcgaa aatcgtctgt tcgtacggca tcactaaatc cttcagattt
    36061 tttctgatcc aaaacaatga agggccggcc ttgttttttc aaattttcgt tgattgcatc
    36121 tgtagagaca gcagaagtcg cgataatgaa tccatccaca cctcgacgtg tgagctcggc
    36181 tagatattcg atttcttttt gatgatcaga atcagcatta cataatatcg tgacgaaatt
    36241 ctgcttatac aagatatcct caattcctcg catcagtgta ctgaagaatg gattcgtgat
    36301 atcaggaacg agtacaccaa ttgttttcgt ttccttggta atcatttgtc tagcaaaata
    36361 gtcaggctga tagcctaatt cttctttggc agctaaaact ttctgaacag ttttcggact
    36421 gaatttctcc gtatttccat ttaatatcaa ggagacagtt gtgattgaaa cgccggaaag
    36481 tttagcgact tctttgattg tgatcttttt tttcaccatg ttcatactcc tatctaaatc
    36541 ctgtggttat tttcccatga aatcaaaaag aagtaaatga aaatcacgtg tgccagtact
    36601 tatttgcttt gttggatagc ttcgattatt tgtttcgttg agcgtactcg tgcagttttc
    36661 ggaaatatct tttttatgaa caattgatgg gattctttgt cacgatcact gcaagcatct
    36721 tcagcaagca cgatatggta cccgtgttga aaggcatcta atgcagtagc atatacaccg
    36781 tttgttgtcg ctacaccgct taaaatgata gtttcgattc cacgccggcg aagttgaaga
    36841 tcaagagaag taccaaaaaa agcacttgga ttgtattttg tgatagtcaa catattatct
    36901 gtatctttca aagaatcctt caaaagtaaa tcagtgaatt ctggaggtac agaagcagtc
    36961 attccaccca tatcagtttg ttgactcaaa taagggaaag tggagatgtc tacgttcact
    37021 aaaactttca aggcagcggt acgtttcaat gttcttacta acaaatcatt gtttgtcaga
    37081 atagatgctg cagtgtgtgg agagagtgta ccgttattta cgatctcttt ttgcatgtcg
    37141 atcgcgacaa aagcagtttt tgataaatca aaaatcatat tttcctttaa atcattcatg
    37201 atgttccttc tttcaaataa caatgttttt tatgtaagta aatcataaat cgtttatttg
    37261 tcaactaaat taaaggattc ctttctttag tctagagaaa ttcagtggta aaataaataa
    37321 gtgtgagaaa acgcattcga tgcatagata tagcgagaaa tatcgtgaaa atggaggaat
    37381 cagagtgaag tatgtcgaag taaaaaatga atacaaacga tacaaaatgg gagaaacgac
    37441 cattacagca aatgacgata tttctttttc aattgaaaaa ggcgaattag tgattatact
    37501 tggtccaagt ggagcaggaa agtcaacgat tcttaatatt ttaggtggga tggatacacc
    37561 tgatgaaggt cagatcatta ttgatgacac agatattgcg caattttcag ataaacagct
    37621 tactgcttac cgacgaacag atgtagggtt cgttttccaa ttctacaatc tcgttcctaa
    37681 tctaacagca aaagaaaatg tggaattggc aacggaagtt tctcctgatg cgcttgatcc
    37741 tgtagaggta ttgaggcaag tgggcctagc tcatcgattg aataactttc cgtcccaatt
    37801 atcaggaggc gaacagcaga gggtttcaat tgctcgtgca ctagctaaaa atcccaaact
    37861 tcttttatgt gatgaaccga caggcgcgct ggattttgaa acgggcaagc aagtattgaa
    37921 attattgcaa aacgctagca gacaacacgg caataccgtt ttgatcatta cacataattc
    37981 tgctttagcg cccatcgctg atagagtcat tcacattaat gatgctaaag taaggtctgt
    38041 tgaattaaat gatcatcctt catccattga tgagattgta tggtaggggg attttatgaa
    38101 aacaagaaca tatcttaaag caggttttcg cgaaattcgg caatctaaag gacgattcat
    38161 tgcaatcatc cttattgtca tgctaggaac acttttattt gtaggtgtca aaactgctgg
    38221 acctgtcatg caaaaaacga tggatcacta tgttggtaca gcaggacttt ctgatttgca
    38281 aatcgtttct actggtggct tgacacaaaa agatattgct caagcagaaa agatcccaga
    38341 tgcaaaagta gaaacaggaa aacagatcta ttattccaat actggcaaaa acgaagtgat
    38401 tcaaatattt tcttataaca aaaataacaa gcagaacaag ctacaattaa ctgatggaaa
    38461 actcccggaa aaaccaaacc agcttgtatt ggatgaaaaa gcggaagatg aagggtataa
    38521 aatcggtgat atttatcaga ttgattcaga tgaattgaaa gaaaaagagt ataccatcac
    38581 gggctttgtc cgttctccat tgttcatcaa caatttggag agaggatacg cgaacgtcgg
    38641 aaatggtagc gtcgattatt ttgtctattt acctgaatcc gtttttaaaa cagatattta
    38701 ttctgtctta tatcttgatt ttacaaacgt aaagaatttg aatacgtaca gcaaagctta
    38761 caaagataaa atggagcaaa atcaagaaaa ggcagaaaag tatttacaag atcgacccga
    38821 agaacgctta gaagagctga aaaaatcggc caacgaagaa ttagaaccgg cacagcaaaa
    38881 aatagcggat ggcaaagcac aaacaacgca agcaagaaca cggttagaag atgctaaaaa
    38941 acagttagcg caacagtcag caactatcca gcagcttcct gaagcgcaac gaacaatcgc
    39001 tgttcaaaca ttatctcaac aagaagctca acttgctgaa caagaaaggc aattagttga
    39061 gaaagaacgt gaactggcct ctgcagagga agaattgaat cgaaatgcag aaaaaattga
    39121 aaatttaact aaaccatcat acctatacaa tgaacgttca gaaaatgtcg gttttcaaga
    39181 atttggtgat ctgtctgatc gtatcgctgc gattgccaat gtttttcctg ttttcttttt
    39241 ctttattgcc gcattgatca cctttacgac aatgacacgt atggtagaag aaaaccgcag
    39301 ggaaatcggg acattaaagg cgttaggtta tacgaaagtt gaaatcgcaa aaaaatatgc
    39361 aatctatgca agtctagcct cagggatagg tattgtttta ggaacgatat taggaacgaa
    39421 tcttttgcca aggatcattt ttgagctgtc taacgagcgg tatgatattg ggagtgcgct
    39481 tattttttat gactggccgc cgattttaca agcagctgcc gcattcttca ttgccgcatt
    39541 tggcgctgca atgatcgttt tattcaaaga tttaagagaa cggcctgctg cattgcttca
    39601 accgaaagca ccgaagccag ggaaacgcat ttttcttgaa tacatcactc cgctgtggtc
    39661 acgccttagc tttaatcaaa aaatcagtta ccgaaatctc ttccgatata agtcaagaat
    39721 gcttatggcg attatcggaa ttgctggatg tgccgggctg atggttgcag gcgtgggatt
    39781 gaaagattct ctaagttctg tttcagccaa acaatttgga ccgatcattg actatcaagc
    39841 aatcgttaca ttggattcat ccacggaaga tagtcgtcaa aaagcagcgg atatgctgca
    39901 gaagcaagaa aaagtaaatg agatcctagc tattcatact caaaccgtag aattgagaaa
    39961 aaaaggacat gcttcgcaaa gtctgacagt gatcgtacca agtaaaacag ctgatttgga
    40021 tcgttttatc catttaactg atcttgaaga gaacccacgc tccttgccta aaacaggaat
    40081 cgctattacg caaaaagtag cagaactatt tgacttatct acaggagatc atatttcgct
    40141 ttatgatgaa gatcaaaaag aatggaaagt cgaagtaaaa gaaatcgtga aaaactatct
    40201 aggtagcttt atttatatgt cccctgaata ttatgcttca gttactgatc aatcgatgat
    40261 ggataatgca tttttgctgc gtacagataa gatgacaaca aaagaagaag aaatgctttc
    40321 cgagaaactg cttgccacag acgctgtgac aaatacgtct ttcatgtcca aacaaatcga
    40381 aacgcaagaa gaatcaatgg cgaacttaga ctcggttgtc ttaatctttg tcgtattatc
    40441 aggactgctg gcttttatcg tcttgtataa tctaactaat atcaatattt ccgagcgtgt
    40501 acgcgaatta tctacaataa aagtccttgg attttttgat aaagaagtca cgatgtacat
    40561 tgttcgagaa aatatcatct ttactctttt aggaatcatc ggaggatttg gtgtaggata
    40621 tatattgact gacttcatct tgcaacaagc gtctatggag aatgtcatct ttccattagt
    40681 gatcacatgg tgggcttatg tcttatcagc aggattaact atcgtattta cggttattgt
    40741 gatgattgtt acccatttca aattaaagca tatcgatatg attgatgcgt tgaaatcgaa
    40801 tgaataaaaa cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg
    40861 caaaatagtt tttcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
    40921 caccatgaat cagaggttgt aaaaaagccg tcctgcatcg agcaaaaaga atactcaaat
    40981 aaaattagct cctcatgttt tacatttgag aagactgatt gccgtagata aaagcttttg
    41041 aacaccattt atagaagaaa caagaatatt ttgctatttt aaacaagagg atagacaaat
    41101 acatgtctga tctcttgttt tttattgggc aacagacctc ttagaatgta taaagataaa
    41161 ttttacaaac gctttcggtt cagttataat aataaatgat aacttgtata tataagttgt
    41221 ggtaggtaat aaatcagttg aatggaggta aatatccatg acatttaaag acaaagtcat
    41281 ttaccaaatc tatcccaaat cttacaaaga cagtaatggt gatggaattg gagatttaaa
    41341 aggaatcaaa gaaaaactcc cgtatctgaa tgatctaggc atcgacatga tttggctaaa
    41401 ccctatttat ccaagccctc aaaaagacaa cggttatgat atctctgatt acacagccat
    41461 cgatcctatc tttggtacga tggaggaatt cgaacagcta atagccgaag caaagaaata
    41521 ccggatagaa atcatgctcg atatggtgct gaatcacgtt tcgacagaac atgaatggtt
    41581 caaaaaagca ctagcagggg accagcatta tcaggacttc ttcattttaa gagaagagcc
    41641 aacggattgg gtatcaaaat ttggtggaag tgcttggtcg ccatttggag atacggggaa
    41701 atattatttg cacttatatg atcggacaca agctgacttg aactggcgga accaagcagt
    41761 aagagaggaa ctattcaaag ttgttcgttt ttggatggaa aaaggggtaa aaggattccg
    41821 tttcgatgtt atcaatgtta tcggtaaaga cgaacaattg aaaaataata aagaaaatga
    41881 tggcaaacct gaatatacgg acaaaccaat cacccatacg tatctaaaag aattgaacca
    41941 agcaacgttt gggacggatt ccactatcat cacagtcggt gaaatgagct ctacgacgat
    42001 cgaaaattgt atcttgtata cagaacctag tagagaagaa ttatcaatgg tctttaattt
    42061 ccatcatttg aaagtcgatt atgaaaatgg gaataaatgg tctactcctc catttgattt
    42121 tgcagaatta aagcgactat ttcatacatg gggagaaaag atgagtgagg gtaacggctg
    42181 gaatgctcta ttttttaata atcatgatca gccacgtgcg ctcaatcgat ttgtagctat
    42241 cgatcagtat cgaaaacaag gagcagagat gctagcaacc atgatccatt tgaaccgtgg
    42301 gacgccctat atttatatgg gagaagaaat cggcatgatc gacccagact attcatcgat
    42361 ttccgactat attgacgtag aaagtttgaa tgcttataag ttactccttg aacaaggatt
    42421 cacagaaaaa gaagcgtttc gtcggatcaa agcaaaatct cgcgataact ctcgaacacc
    42481 catgcaatgg tcagacacag aacaagcagg atttactaca gggaaacctt ggttaaaagt
    42541 tggaggcaag ctagaagaga ttaatgtaga aaaagaacta tcttctgaag attctatttt
    42601 gtcctattac aagaaattga ttcggctgag aaaaacatat ccaatcgttg cacaaggaga
    42661 ttatcatgct tatgaagcaa atcatccgga agtttatggc tttcttcgcc aatttgaagg
    42721 acagcagctg cttgtattaa caaatttcta tgataaagat acaaggatcg ccatcccaga
    42781 aggattcaca gaagccaata ttcttatcga taattattcc gatactgttc ttcatcaaag
    42841 tctggagcta aaaccatacc aggcaattgc tttgctgaaa aattaaaaca aaaacacaaa
    42901 acgtcgttcc tacgagctat tctcgtaaga tcgacgtttt gtgttttatt taaaataatc
    42961 ttcttcagaa aatgaagagt tttcatactc tcctttatcg ccataaaatt tattgtagcg
    43021 ttgtttgaat ttacggaaaa taaaagtaac gagtaatttg actaacgtat agataggaat
    43081 accaaaaata acgcccacaa ttcccattag atcacccatg acaagtaaaa cgattaagat
    43141 cgtaatcgga tggatctgta aacgatcacc cattactcga ggaatcacca agtcaccgtg
    43201 gagaagttgt acggcaaacc agacaatgac aaatttgaca gccataaaag tagagtcttg
    43261 gaatgcaatg atcaaacctg gaaagaaggc gataaacggc ccaatatatg gaatgataca
    43321 taacaaccca gcagaaatag ctaaaacact agcaaattct aatccgatca gtaagaaaca
    43381 aacccagtag actgctccaa ggataaatga agcaatgatt tgccccttta gaaatgaacc
    43441 caattgttga ttagcaatct ttgtcaaatt tttaaaatct gtacgaaatg ctggtgggac
    43501 aatcgctaaa actgactgat aaaatttttg aggatctctc aaaaggaaaa aagcgacaat
    43561 tggaccagta aataaagtaa taaaaatagc tgtaacagtt gaaaaaatgt tgctgatccg
    43621 ttgtgcgcca ctttcccaat aatttccaat aaatccagct atatcagacg tcacgtcgtc
    43681 aatagaagcg aaaaattgat taaatgaatc ggcaaaaggt gttttctcca aaaattgtcg
    43741 gagactctct tgaaaatctt gaaacaaatc tggcaaacta ctgatcaatg tctttgcctg
    43801 ttcttcaaga aaaggaataa gccatatgct tccaagcccc aataaaaata aaataatcac
    43861 ataaacgata atgactgaca agttcctcga aaggtgcttg ctcaaacgtt tgatgactgg
    43921 ttccaaaaga taatacaaga tcagtgcaaa caataatggg ggcaagatag tagcgataat
    43981 gattccgaca ggcttgaata taaacgagat ctgatggaaa aaataaatga tcagcgcaag
    44041 taagataact attcctaaaa catagtaaga ggtttttcct cctaaaaagc ggatcaaacg
    44101 attcgttgta tctgtttctt ttttcactct ttttcctcct ttatctataa cttcattata
    44161 gacaaaagcg ggtataaaag gaaaatgata gaggttgatt gcaaaaatat cgtcaatcat
    44221 tcatataaac tttctcatca attaaaataa gaaaacgctt gacagttctt ttaaccatat
    44281 gataaattaa atcctgttgc taaaacgtaa ctattacggt ttgcgaaaag cgaatcggaa
    44341 cattcaaatg aaagaagatg agaagaggag tgtcataaat gaaacgattg ataggtatat
    44401 tggccatgct ggttttagca ggtatgttaa ctgcttgcgg ggcaagtggg aaagccgagg
    44461 acagtaaaga aaaattgagc gtgatgacga ccttttatcc catgtatgat tttacaaaag
    44521 caatcgtcgg ggatgaagga gaagtagagt tattgattcc agcagggact gattcccacg
    44581 attatgaacc ttctgctaaa gacatggcaa agatccaaga tacagatatc tttgtttata
    44641 atgatgaaaa catggaaaca tgggtccccg caatccaaaa aacattacaa gaaggcaatg
    44701 tccatacgat aaaagcgaca gaaggtatgc ttttacttcc gggaagtgaa gaaggacatg
    44761 atcatgacca tgaacacggg gaagaaggcc atacacatga gctagaccca catgtatggc
    44821 tagctccaag tttagcaatc aagcaggtag caaacatacg tgatcagctg attgaagctt
    44881 acccagaaaa acaggaagta tggacaaaaa atgcagcagc ctataccgag aaacttcaag
    44941 cactccatca gctttatcaa gaaaccttta agcaagcaaa acaaagatct tttgtcacgc
    45001 aacatgctgc attcaactat ctagcgttag aatatggatt gaatcaagta tcgattgctg
    45061 gtctttcttc tagtgaagaa ccgtctgcgg cgagaattgc tgaactgaaa cactttgtta
    45121 aagaacatgg aatcaactat atttattttg aagaaaacgc caaagatagt attgccagaa
    45181 cgttagcaaa cgaagcaggt gtatctttag aagtattgaa tcctttggaa ggattgacga
    45241 atgaacaaat agaaaatgga gaaaactatc tttctatcat ggaagcaaac ctcgaagcgt
    45301 tgaaaaaaac gacagagaca gaaaacccgc tagaagatac tttgaccccc aaaaaagaaa
    45361 agagcgtcta caacggttat tttgaagacg aggatgtaca agatcgctct ctttcagatt
    45421 gggcaggaac ttggcaaaca gtagatacgt atgtagaaga tgggactttt gatcctgttt
    45481 ttgaatataa agaaaaattg aatcaggata agacagcaca agaatataaa gaatattata
    45541 cgaagggcta tcaaacagat attgcttcaa tcaagatcag tgagaaagag atggcattta
    45601 cgtttaagaa tggaacaacg aaacgttcaa attatcgtta tgtagggaaa gaaatcttga
    45661 cctacgaggc aggaaaccgt ggcgtaaggt atctattcga agcagaagat aaggaaagtg
    45721 gcgcttttag atatattcaa ttcagtgatc acacgattgc accggctgac tccgaacatt
    45781 ttcatttata tgcaggaa
//
LOCUS       DAYYFD010000008        45039 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000008 DAYYFD010000000
VERSION     DAYYFD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.008"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..2685
                     /gene="adhE"
                     /locus_tag="ACHA25_000425"
                     /gene_synonym="adhC"
     CDS             88..2685
                     /gene="adhE"
                     /locus_tag="ACHA25_000425"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8761758.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2914..4020
                     /locus_tag="ACHA25_000426"
     CDS             2914..4020
                     /locus_tag="ACHA25_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8761759.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4037..5065
                     /locus_tag="ACHA25_000427"
     CDS             4037..5065
                     /locus_tag="ACHA25_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8761760.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5071..5550
                     /locus_tag="ACHA25_000428"
     CDS             5071..5550
                     /locus_tag="ACHA25_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8761761.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5566..6861
                     /locus_tag="ACHA25_000429"
     CDS             5566..6861
                     /locus_tag="ACHA25_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8761762.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6872..7696
                     /locus_tag="ACHA25_000430"
     CDS             6872..7696
                     /locus_tag="ACHA25_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8761763.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7861..8760
                     /locus_tag="ACHA25_000431"
     CDS             7861..8760
                     /locus_tag="ACHA25_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8761764.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8886..9755)
                     /locus_tag="ACHA25_000432"
     CDS             complement(8886..9755)
                     /locus_tag="ACHA25_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8761765.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9908..11368
                     /gene="rhaB"
                     /locus_tag="ACHA25_000433"
     CDS             9908..11368
                     /gene="rhaB"
                     /locus_tag="ACHA25_000433"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8761766.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11365..12621
                     /gene="rhaA"
                     /locus_tag="ACHA25_000434"
     CDS             11365..12621
                     /gene="rhaA"
                     /locus_tag="ACHA25_000434"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8761767.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12635..13480
                     /gene="rhaD"
                     /locus_tag="ACHA25_000435"
     CDS             12635..13480
                     /gene="rhaD"
                     /locus_tag="ACHA25_000435"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8761768.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13482..13793
                     /gene="rhaM"
                     /locus_tag="ACHA25_000436"
     CDS             13482..13793
                     /gene="rhaM"
                     /locus_tag="ACHA25_000436"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8761769.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13875..15026
                     /gene="fucO"
                     /locus_tag="ACHA25_000437"
     CDS             13875..15026
                     /gene="fucO"
                     /locus_tag="ACHA25_000437"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8761770.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15153..15911
                     /locus_tag="ACHA25_000438"
     CDS             15153..15911
                     /locus_tag="ACHA25_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8761771.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16011..16655
                     /locus_tag="ACHA25_000439"
     CDS             16011..16655
                     /locus_tag="ACHA25_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8761772.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16662..17669
                     /locus_tag="ACHA25_000440"
     CDS             16662..17669
                     /locus_tag="ACHA25_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8761773.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17685..18527
                     /gene="kduI"
                     /locus_tag="ACHA25_000441"
     CDS             17685..18527
                     /gene="kduI"
                     /locus_tag="ACHA25_000441"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8761774.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18547..19350
                     /locus_tag="ACHA25_000442"
     CDS             18547..19350
                     /locus_tag="ACHA25_000442"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8761775.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19599..20663)
                     /gene="fni"
                     /locus_tag="ACHA25_000443"
     CDS             complement(19599..20663)
                     /gene="fni"
                     /locus_tag="ACHA25_000443"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8761776.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20660..21745)
                     /locus_tag="ACHA25_000444"
     CDS             complement(20660..21745)
                     /locus_tag="ACHA25_000444"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8761777.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21758..22735)
                     /gene="mvaD"
                     /locus_tag="ACHA25_000445"
     CDS             complement(21758..22735)
                     /gene="mvaD"
                     /locus_tag="ACHA25_000445"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8761778.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22728..23672)
                     /gene="mvk"
                     /locus_tag="ACHA25_000446"
     CDS             complement(22728..23672)
                     /gene="mvk"
                     /locus_tag="ACHA25_000446"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8761779.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23988..24647
                     /locus_tag="ACHA25_000447"
     CDS             23988..24647
                     /locus_tag="ACHA25_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8761780.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24732..25637
                     /locus_tag="ACHA25_000448"
     CDS             24732..25637
                     /locus_tag="ACHA25_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8761781.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25639..26271)
                     /locus_tag="ACHA25_000449"
     CDS             complement(25639..26271)
                     /locus_tag="ACHA25_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8761782.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26422..26547
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26591..27115
                     /gene="infC"
                     /locus_tag="ACHA25_000450"
     CDS             26591..27115
                     /gene="infC"
                     /locus_tag="ACHA25_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8761783.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27187..27387
                     /gene="rpmI"
                     /locus_tag="ACHA25_000451"
     CDS             27187..27387
                     /gene="rpmI"
                     /locus_tag="ACHA25_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8761784.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27440..27799
                     /gene="rplT"
                     /locus_tag="ACHA25_000452"
     CDS             27440..27799
                     /gene="rplT"
                     /locus_tag="ACHA25_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8761785.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28051..29388
                     /locus_tag="ACHA25_000453"
     CDS             28051..29388
                     /locus_tag="ACHA25_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8761786.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29436..31565
                     /locus_tag="ACHA25_000454"
     CDS             29436..31565
                     /locus_tag="ACHA25_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8761787.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31540..32229
                     /locus_tag="ACHA25_000455"
     CDS             31540..32229
                     /locus_tag="ACHA25_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761788.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32637..33314)
                     /locus_tag="ACHA25_000456"
     CDS             complement(32637..33314)
                     /locus_tag="ACHA25_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8761789.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(33450..33644)
                     /locus_tag="ACHA25_000457"
     CDS             complement(33450..33644)
                     /locus_tag="ACHA25_000457"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761790.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33694..34134)
                     /locus_tag="ACHA25_000458"
     CDS             complement(33694..34134)
                     /locus_tag="ACHA25_000458"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8761791.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34138..34983)
                     /locus_tag="ACHA25_000459"
     CDS             complement(34138..34983)
                     /locus_tag="ACHA25_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8761792.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35132..36550
                     /gene="pepV"
                     /locus_tag="ACHA25_000460"
     CDS             35132..36550
                     /gene="pepV"
                     /locus_tag="ACHA25_000460"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8761793.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36607..37554)
                     /locus_tag="ACHA25_000461"
     CDS             complement(36607..37554)
                     /locus_tag="ACHA25_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8761794.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37777..38127)
                     /locus_tag="ACHA25_000462"
     CDS             complement(37777..38127)
                     /locus_tag="ACHA25_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8761795.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38327..39406
                     /gene="pepA"
                     /locus_tag="ACHA25_000463"
     CDS             38327..39406
                     /gene="pepA"
                     /locus_tag="ACHA25_000463"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8761796.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39550..39870
                     /locus_tag="ACHA25_000464"
     CDS             39550..39870
                     /locus_tag="ACHA25_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8761797.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39892..40356
                     /locus_tag="ACHA25_000465"
     CDS             39892..40356
                     /locus_tag="ACHA25_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8761798.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40561..41166
                     /gene="ytpR"
                     /locus_tag="ACHA25_000466"
     CDS             40561..41166
                     /gene="ytpR"
                     /locus_tag="ACHA25_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8761799.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41205..42494)
                     /locus_tag="ACHA25_000467"
     CDS             complement(41205..42494)
                     /locus_tag="ACHA25_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8761800.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42922..43401
                     /gene="rlmH"
                     /locus_tag="ACHA25_000468"
     CDS             42922..43401
                     /gene="rlmH"
                     /locus_tag="ACHA25_000468"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8761801.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43604..43729
                     /locus_tag="ACHA25_000469"
     CDS             43604..43729
                     /locus_tag="ACHA25_000469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761802.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            43726..44154
                     /locus_tag="ACHA25_000470"
     CDS             43726..44154
                     /locus_tag="ACHA25_000470"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8761803.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            44225..>45039
                     /locus_tag="ACHA25_000471"
     CDS             44225..>45039
                     /locus_tag="ACHA25_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8761804.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICS"
BASE COUNT        14328 a         8140 c         9592 g        12979 t
ORIGIN      
        1 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
       61 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
      121 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
      181 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
      241 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
      301 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
      361 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
      421 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
      481 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
      541 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
      601 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
      661 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
      721 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
      781 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
      841 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
      901 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
      961 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     1021 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     1081 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     1141 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     1201 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     1261 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     1321 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     1381 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     1441 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     1501 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     1561 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     1621 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     1681 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     1741 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     1801 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     1861 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     1921 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     1981 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     2041 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     2101 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     2161 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     2221 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     2281 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     2341 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     2401 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     2461 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     2521 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     2581 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     2641 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     2701 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     2761 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     2821 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     2881 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     2941 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     3001 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     3061 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     3121 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     3181 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     3241 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     3301 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     3361 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     3421 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     3481 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     3541 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     3601 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     3661 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     3721 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     3781 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     3841 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
     3901 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
     3961 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
     4021 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
     4081 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
     4141 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
     4201 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
     4261 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
     4321 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
     4381 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
     4441 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
     4501 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
     4561 ggaagatttg aagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
     4621 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
     4681 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
     4741 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
     4801 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
     4861 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
     4921 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
     4981 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
     5041 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
     5101 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
     5161 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
     5221 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
     5281 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
     5341 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
     5401 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
     5461 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
     5521 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
     5581 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
     5641 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
     5701 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
     5761 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
     5821 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
     5881 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
     5941 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
     6001 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
     6061 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
     6121 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
     6181 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
     6241 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
     6301 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
     6361 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
     6421 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
     6481 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
     6541 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
     6601 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
     6661 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
     6721 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
     6781 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
     6841 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
     6901 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
     6961 tacactgcag ctcgccttag gtctttcgtt tcctgaaata ccttctggcg caaatgagat
     7021 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
     7081 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
     7141 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
     7201 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
     7261 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
     7321 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
     7381 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
     7441 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
     7501 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
     7561 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
     7621 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
     7681 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
     7741 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
     7801 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
     7861 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
     7921 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
     7981 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
     8041 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
     8101 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
     8161 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
     8221 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
     8281 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
     8341 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
     8401 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
     8461 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
     8521 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
     8581 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
     8641 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
     8701 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
     8761 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
     8821 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
     8881 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
     8941 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
     9001 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
     9061 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
     9121 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
     9181 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
     9241 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
     9301 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
     9361 tctcctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
     9421 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
     9481 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
     9541 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
     9601 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
     9661 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
     9721 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
     9781 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
     9841 ttgtcaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
     9901 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
     9961 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    10021 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    10081 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    10141 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    10201 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    10261 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    10321 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    10381 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    10441 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    10501 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    10561 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    10621 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    10681 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    10741 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    10801 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    10861 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    10921 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    10981 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    11041 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    11101 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    11161 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    11221 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    11281 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    11341 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    11401 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    11461 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    11521 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    11581 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    11641 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    11701 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    11761 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    11821 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    11881 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    11941 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    12001 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    12061 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    12121 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    12181 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    12241 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    12301 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    12361 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    12421 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    12481 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    12541 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    12601 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    12661 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    12721 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    12781 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    12841 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    12901 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    12961 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    13021 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    13081 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    13141 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    13201 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    13261 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    13321 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    13381 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    13441 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    13501 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    13561 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    13621 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    13681 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    13741 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    13801 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaat aatacaaatt
    13861 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    13921 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    13981 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    14041 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    14101 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    14161 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    14221 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    14281 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    14341 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    14401 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    14461 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    14521 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agcttctgta gatggcgatc
    14581 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    14641 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    14701 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    14761 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    14821 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    14881 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    14941 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    15001 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    15061 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    15121 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    15181 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    15241 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    15301 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    15361 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    15421 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    15481 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    15541 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    15601 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    15661 atacgatcac caatgcatta cggttgaaaa aggaaatcaa aacggtaaaa gaaacccaca
    15721 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    15781 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    15841 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    15901 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    15961 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    16021 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    16081 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    16141 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    16201 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    16261 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    16321 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    16381 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    16441 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    16501 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    16561 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    16621 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    16681 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    16741 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    16801 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    16861 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    16921 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    16981 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    17041 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    17101 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    17161 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    17221 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    17281 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    17341 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    17401 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    17461 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    17521 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    17581 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    17641 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    17701 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    17761 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    17821 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    17881 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    17941 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    18001 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    18061 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    18121 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    18181 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    18241 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    18301 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    18361 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    18421 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    18481 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    18541 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    18601 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    18661 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    18721 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    18781 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    18841 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    18901 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    18961 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    19021 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    19081 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    19141 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    19201 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    19261 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    19321 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    19381 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    19441 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    19501 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    19561 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    19621 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    19681 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    19741 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    19801 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    19861 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    19921 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    19981 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    20041 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    20101 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    20161 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    20221 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    20281 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    20341 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    20401 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    20461 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    20521 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    20581 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    20641 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    20701 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    20761 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    20821 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    20881 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    20941 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    21001 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    21061 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    21121 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    21181 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    21241 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    21301 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    21361 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    21421 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    21481 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    21541 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    21601 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    21661 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    21721 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    21781 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    21841 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    21901 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    21961 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    22021 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    22081 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    22141 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    22201 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    22261 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    22321 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    22381 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    22441 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    22501 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    22561 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    22621 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    22681 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    22741 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    22801 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    22861 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    22921 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    22981 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    23041 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    23101 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    23161 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    23221 gtagcggctg catcgattcc actaggatta ccatgggcga ttttttccga aagctggaca
    23281 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    23341 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    23401 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    23461 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    23521 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    23581 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    23641 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    23701 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    23761 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    23821 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    23881 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    23941 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    24001 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    24061 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    24121 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    24181 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    24241 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    24301 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    24361 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    24421 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    24481 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    24541 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    24601 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    24661 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    24721 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    24781 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    24841 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    24901 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    24961 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    25021 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    25081 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    25141 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    25201 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    25261 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    25321 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    25381 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    25441 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    25501 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    25561 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    25621 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    25681 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    25741 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    25801 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    25861 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    25921 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    25981 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    26041 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    26101 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    26161 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    26221 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    26281 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    26341 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    26401 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    26461 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    26521 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    26581 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    26641 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    26701 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    26761 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    26821 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    26881 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    26941 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    27001 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    27061 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    27121 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    27181 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    27241 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    27301 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    27361 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    27421 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    27481 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    27541 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    27601 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    27661 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    27721 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    27781 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    27841 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    27901 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    27961 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    28021 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    28081 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    28141 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    28201 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    28261 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    28321 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    28381 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    28441 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    28501 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    28561 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    28621 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    28681 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    28741 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    28801 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    28861 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    28921 cagtttgcga aattatttac agatggcgca gtagatgttg cgccaatggt tggtttcttg
    28981 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    29041 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    29101 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    29161 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    29221 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    29281 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    29341 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    29401 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    29461 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    29521 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    29581 ttcagtcttt tcaaaattgt ttgctggaaa ataatatcag tccagaagat gttgtgtttg
    29641 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    29701 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    29761 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    29821 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    29881 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    29941 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    30001 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    30061 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    30121 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    30181 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    30241 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    30301 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    30361 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    30421 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    30481 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    30541 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    30601 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    30661 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    30721 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    30781 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    30841 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    30901 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    30961 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    31021 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    31081 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    31141 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    31201 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    31261 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    31321 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    31381 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    31441 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    31501 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    31561 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    31621 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    31681 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    31741 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    31801 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    31861 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    31921 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    31981 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    32041 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    32101 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    32161 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    32221 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    32281 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    32341 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    32401 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    32461 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    32521 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    32581 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    32641 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    32701 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    32761 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    32821 tctgaaatcg tcaaacgtaa ccatctgaaa cgcgtactat aactttctag ccaatcccaa
    32881 atttctcgtt ttccagcaat ttcataacaa gttcggtgaa aatcagtatc caaatcaaaa
    32941 aaagcttttt tattttgagc gattactgcc tgatcggttt gttctagtat tttttctagt
    33001 ttagcttgat cttgcttggt cattttcgca ctgcattctt gcaggatcgg tttttccaac
    33061 ttttcccgcg tataacgggc taatgaagca gatcggatat cgatttttga aacataagtg
    33121 ccggactgtg gaatggtttt tacaagctct tgattgcgca gttggatcag tgcttctcga
    33181 atcggtgtac gcccgatagc tagttgttct tctaacactt tatcggagat tttacttcca
    33241 ggtgctaact cggaataaat gatttgttta cgtatagatt cataagcttg ctgttgcaaa
    33301 ttattgataa ccatttaacc ttcctttcta tgcagatata atctattata acataaatcg
    33361 cttacaaatc gtgtgggcat aaaaacacag tctgcaaaaa aagccctgat aggcatgcct
    33421 attagagcct cgaaaagaaa aatattgaat caatctggtc gtacctcttg gatcggtttg
    33481 acacgaccgc cgattctttt agcaaaagct tcggattctt ttttgtcttt gaaaatgagc
    33541 agattgtcta agtttttttc ccgagtgcga cagccagttt tatcgatgta accgtgcatt
    33601 actttaacga catacatggg gatcacctgc ttttttcttt tcattatacg aactactcga
    33661 cacaatacaa gaaaaatgct tagacggtct ttattaagaa tttacatcat ccaaagaagc
    33721 gtacgcttgg aataactgat ctaactccgg attgactttg ctcagcttca ctaatcgttg
    33781 cgaatcagaa agtaaaaaac agtgtttact actgccagta gtttttaatt ttttgtcttc
    33841 agttcccaca atttcgtagc taaaatccaa ccgtgtcccc gtatactttt ggatagttgg
    33901 atggatgctc actttttctc cgtaatggat catttcttta tagctgcaat ttacttccag
    33961 aacggggatg atgatcccag attcttcgat ttttttatag ggaaacccta gatgtaccaa
    34021 taaggctacc cgagcctctt caaaccagcg gatataattg ctgtgatgaa taatccccat
    34081 ttgatctgtt tcataataaa aaggttctcg cacataacct gtaaatttct ccatgactca
    34141 tgcctcgttt tccattgatt ttttacaaaa ataattcatc catttcggaa gtgcttcgct
    34201 gatttgtgtc ggcaaaacaa tatattgttc ttttgccagc tggtctccaa tgaaactatg
    34261 aagataaata gctgctgcaa tcgtttggtc attttgagga aactgggcta aaaatcctgt
    34321 gatcatccca gctaaagtgt cacccatgcc gcccgttgcc atagcagcag aacctaaagg
    34381 attggtgtag aagtgctggt gaccataaat ttttgtctca tgacttttca aaacgagcac
    34441 agcaccgatt tcctgttgct tctttttgtt ttctttatcc cgttgttctt gtatcggtag
    34501 agaactcaac cgttgccact ccatttgatg aggtgtaagt acagtttgtt cgggataggg
    34561 tagcaaaaga tgattagcag caagtaaact taaagcagag ccatcaatca ccagccattg
    34621 ttcttttgat tggttttcta gtacttgttt gaaacgttcc aaactcttag aagaagtacc
    34681 caatccggga ccaatcaaga gtacatctgc ttgctttatt acggaaagaa actggtcagt
    34741 atccgtccaa actacaatca ttgcttctgg cagatgactg tgaagagcac tccgattttt
    34801 ttcatcagtt actacagtcg tgagacccgc tccgctgtgt acggcagcta aagcactcat
    34861 aatgattgct ccgccatatt gttcattgcc gccaatcaaa acgatacgtc caaatgttcc
    34921 tttatgtgaa atttttggac gaggtgtgat gactcttttc actagttgtt cgtttatcag
    34981 catctaaaaa cctcctttta ctcattatag cgtatatttt ttttagagaa aaacagataa
    35041 gaaaaggaat tgtcagattt tttgaactat ttttttctgt aatttgtggt atcatgaaca
    35101 cgaagactta aataagtgga ggattaactt tatgacaatt gattggcaaa aagaagtaga
    35161 agcacgtaaa gaagacttgc ttgaagactt gaaaaatctt ttacgtgtca acagcgagcg
    35221 cgatgactca aaagtaacac cagatgcacc atttggacct ggacctcgcg acgcattgaa
    35281 acatatgtta gcttatggcg aacgtgatgg atttaaagta aaaaatgtcg ataactatgc
    35341 agggcatatt gatcttggcg aaggagacga aacgctaggg atatttggac acatggatgt
    35401 cgttcctgca ggagatggct gggacacaga tccttatgaa ccagtaatca aggatggaaa
    35461 gatctttgca cgcggatcaa gcgatgacaa aggtccaagt atggcagctt actatgcgat
    35521 gaagatcatt aaagaacttg acttgaaact atcaaaaaaa gttcgcttcg tagtcggcag
    35581 cgatgaagaa agcggctggg gcgatatggc ttattatttc gaacatgaag aagaaccaga
    35641 ttttggtttc tcaccagatg ctgaattccc aatcatcaat ggtgaaaaag gaaatgtttc
    35701 tttagcttta cgcttcaaag gcgacaatgc aggagattat gtcttgaaat cttttgtttc
    35761 cggacttcgc gaaaatatgg tgccaggaac agcaactgca gcacttcaag taccaagtgc
    35821 agatgcagct atcgcgatgg aagaagcatt ttaccaattc atcgaagcaa atccagtaag
    35881 cggaacgatt gaagcggaca atacgtatgt aaaaatcgaa ttagttggta aaggcgcaca
    35941 tggtgcaagc ccacaatctg gtatcaatgc aggttctttc ttggcattgt tcttagataa
    36001 ctatgaattc ctaggatctg ctaaacaatt tatccatgta gcagctgctt atgtccatga
    36061 agatttttat ggagaaaaac taggagtcgc ttatgaagat gaaaaaatgg gcaaattgac
    36121 aatgaatgct ggactatttg cttttgaaga aaatggtaca gaggaagcga acttcatcaa
    36181 tatgaatttc cgttttccaa aaggtgtaac agtagacgga ttgcaaagtg acatcgaaca
    36241 aacagtgggg caagaaggcg caacagttac tcgaggtgct cgcgtaatgg aaccacatta
    36301 cgtaccaatg gatgatccat tggtggctac attattgcaa gtctatgaag accacactgg
    36361 agaaaaaggc tacgaacaaa tcatcggtgg cggaacttac ggacgtttgt tgaaacgtgg
    36421 cgttgcttat ggagcgatgt tcccaggata tactgataca atgcatcaag caaacgaatt
    36481 tatgagtctt gatgacttgt tcagagcaac agcaatctat gcagatgcca tctatcgttt
    36541 ggcgaaataa ttaattaaaa agaggatcaa gaattgcaaa ttatttttgc agtcttgatc
    36601 ctttttttac tttctactgt ggtattcatg ataaacatga tatagattca attcatattc
    36661 tttcttagcg ttcgtgacga ctgtatccaa aatcaaccct gttactaatg aaagaagtgc
    36721 taaagtcatc aatccagtac ttaaaatcgc agaagggatg cgtgtaataa agcttgtcag
    36781 catatattcg cgaatcacag gaatccccgt gaccaatcca aataaaaaga agaagatggt
    36841 ccaaatccca aagaaaagca aaggcttata atctttaaac attttaataa tcatcatgat
    36901 cactttgaat ccgtcagaga atgtattcaa ttttgattcg ctgccttcag gacgatcacg
    36961 ataatcgatt gggatttcaa cgaacttgaa ttttttatct aaagcatgga tcgtcaattc
    37021 cgtttcgatt tgaaaaccag aactcataat aggaaaactt ttgacaaaga tacgattgaa
    37081 tcctcgatag ccggtcatga catcaagaat ttttgtttta tacattctag taattgtatt
    37141 tctaacaagg ttatttccaa aatcgtggaa aggtctctta ttctcatcaa agtaggttcc
    37201 atttgatagg cgatcaccaa tgaccatatc tgcttcacca gaacgtaatt tctcgagcaa
    37261 accatgtaca gcttctgcag gataagtatc atcgccatct accattaaat aatagtcggc
    37321 atcgatatca aagaacattt ggcggatcac atttcctttt ccttgacgag gttctttttt
    37381 tacgattgct ccaccttgaa cggccaattc gtatgttcga tcggtcgaat tattatcata
    37441 gacataaata tctgcttctg gtaattctcg tttaaagtct gcaatgactg ttgaaattgt
    37501 tgcttcctca ttgtaacaag gaatcagaac agcaattttt tcttgttgtt ccatttttca
    37561 ctctccttaa ctatagaaaa caaaagctaa attaaaaata cctcataatc aaaggaaatg
    37621 ctagtttttc ccgctaaata tcatttattt aaaaagaaaa tactggaata tatgtttgga
    37681 aagctctatc taaaaaacca cccactacaa aagaaggaca tgatacctct cttttgaata
    37741 gtgggtgcgt attcgttctc tcactttttt gacgtattaa tctgttgcac ctttttttct
    37801 tgaaatttct aatacggttc ctgtatgagc atctgctaaa aattcataat aaacagtttc
    37861 gccatcttct attcgagaaa gacctccccg ataagcactc gaccgaacgg cgaatttcct
    37921 aataggttcc ttttcaaaag agatccaaga accttcgatt gcaccttctt ttagaaaaga
    37981 tgacttgata ttttcaagta cttggtctgc tgacaatact tttttgcgat gataccaaat
    38041 cgttgaagct accccgctca ataatcctag accgattcca actgctaacc cgcctttaaa
    38101 atagcttagt tcgctttctt catacatgtg gacatacctc cttcttctca cttgttactc
    38161 cttgtgctta ttttaccaca ctctcatatt ttctttgaaa taaataaccg ctaccagaaa
    38221 aaataagcta taataaatac gatcggtctg cagaaaaaat catattttat ccgcagatca
    38281 atataagtct gagaggagtt tagtctgaaa tttagacgaa tagcatatgg aagaaaaaac
    38341 atttcaacga atcaaagagt taacagaatt acaaggaaca agcggttttg aacaagatgt
    38401 tcgcgcttat atggaaagtc atatggaacc gctggtagat gagttacagt tagatggttt
    38461 gggcggcatt tttggtctgc gccatcatga agaagcggat gcaccccgtg taatggtcgc
    38521 agcacatatg gatgaagtcg gattcatgct gacacaaatc caagaaaacg ggctttttaa
    38581 agtcgtacct ctaggcggct ggaatcctta cgttgtttct gctcagcgtt ttacacttaa
    38641 aacctctact ggtaagaatt atccatgtat ttcttcttcc gttcccccac atttattgcg
    38701 aggaacaagc ggacaaaaat cagtagaagt aaccgatatt ttgttcgatg ctgggtttga
    38761 atcaagagaa gaggcaatga gttacggtgt ccttccagga gacacaatcg ttccttacgc
    38821 tgaaacgatc aaaacagcca acggaaagaa tattatcagt aaatcatggg acaaccgcta
    38881 tggctgtaca atggtattag aagctttaga agcgttgcaa aatgaaacat taggtcatac
    38941 attgattgca ggcgcgaatg tccaggaaga agtaggcttg cgtggttcta aagcttctgt
    39001 caataaattc aagccagatc tgttctttgc agtagactgc tctgcagcag atgatacagt
    39061 gacgaaaaat ggcacattcg gacatttagg agaaggtaca ttgatgcgta tccaagatcc
    39121 aggtttgatc atgcttcctc gtttgcgcga gtacttgctg gatatcgctg aaacaaacaa
    39181 tatcccttat caatactttg tttcaaaagg cggtacagat gcaggtgctg ctcatacaca
    39241 aaacgaagga atcccaagta cagtgattgg cgtagtggga cgttatatcc atacgcatca
    39301 aacgatgttc agtattcgcg attttgaagc tgcacgagaa atgttgattc aaacactgaa
    39361 aggtttggat aaatcaacag taaatacgat cgtatacgga aaataatgcg atagtgaaac
    39421 tataaaatct ggcaaaagat tgctttttgc cagatttttt ttataaaaag aggatcaatg
    39481 tttgttagtt cgaaagaaag gtgtataatt taattgaaaa atacataaac agaaagtaga
    39541 ggacaaagaa tgattatccc aaatagttta gaagaattag caggctacgt agaaactgga
    39601 aagagtgtat ttttctttac tgccggctgg tgcggagact gccgttttat caaaccgcaa
    39661 atgcctgaaa ttgaaaatga cttttctgat tggcaattta ttgaagtaga ccgtgacaag
    39721 tacatagatg tagccgctga atggaacatt tttggtatcc caagttttgt cgtgatccaa
    39781 gacggaaaag aattaggtcg tctagtgaat aaagatcgta aaacaaaaca ggaaatcgaa
    39841 tcgttcctag aaagtgtaac aaaaggctga catctgttag gaggggtcaa gatgaatcaa
    39901 gaatataaga atattttagt aggtatcgac ggtagtgaac aagcacttgc tgctttcaaa
    39961 aaagcggtag aagtggctcg ccgtaataat gggaaagtat atgtagcaaa cgtaatcgat
    40021 cagcaatttt ataactttat gggctatgca cctattgatc aaaatctagt cgatcagcaa
    40081 actgaaacag ccaaggaaat gatcgaagaa tgtaaagaat atgggaaatc tgttgattac
    40141 acaaatatag aaggaattat tgcatatggt tctgttcgtg aatccatggc agtcaaattg
    40201 ccaaaaaaat atgagatcga tctaatcatg gttgggcagt caggtctaaa tgcagtcgaa
    40261 cgctttatga caggaagtgt agccagctac gtgatacgtc attcaccttg tgatgtcttg
    40321 atcgtttctg acgaagaaga acagccaagt aattaagcaa gtggaacttg tacgaccttt
    40381 ttaatcaatt aactaaaaca gtaaagggac tgtgacaaaa gtcttgtcac agtcccttgt
    40441 tacgaataaa tggtgttcca gaagaggctt cttcggaaat aaagcatgaa aaaaatttca
    40501 tgcagtgaat gcaatttttt gttaagataa atctcagcaa aggataatgg aggaaagaac
    40561 atgatttttg cttataacaa agcacacgtc ggagacacct tgatggttat tatagccgac
    40621 gataaaggaa cagaaaacac tgttgaacgc aaatggaatg ttgctcggat caaagatgaa
    40681 caaggtcaag ttgttgcttg gaactttttc catatctccg atcatctaac aatcgaaggg
    40741 aacggccaag taagcttaac agatgaggaa ttagcgaaat tgaatcgatt gatcaaagaa
    40801 gcaggattcg aagaacagct tgaagctgat catgaaccga agatcgttgt cgggtacgta
    40861 aaaacatgcg tcccacatcc tgattctgac catttatcga ttactgagac agaagtagac
    40921 aatggtacag tattgcaaat cgtttgcggt gcacccaata tcgaagctgg acaaaaagtt
    40981 gtcgttgcaa aaccaggagc tatgatgcca gatggaatga tgatctggcc aggaactttg
    41041 cgaggggtag atagctatgg aatgatctgt tccgctcgtg aattgcattt gccaaatgct
    41101 ccagcaaaaa aaggaatctt agtattgcct gaggatgccg tagtcggaga agcatttcca
    41161 aaataaagag gttgtgacaa gtctttgtca caacctcttt tttattaatt ttcactattt
    41221 tgattttgtt ttaatgccga ttgatcgatc gtcaattcaa cagtagttgt tttttctttc
    41281 gagccttcat agtaagtgat tttgacagaa tctcccactt ttttcttata caaagcagat
    41341 tgaagttcta caccagaaga aacttctttg tcatcgatct tagtaatgac gtcgtattgt
    41401 ttcaatccag ctttttcagc cggtgtagct gtttggacat ttgtcacgat gacaccgtta
    41461 gttacagatg aaggaatctt caagatttgt tcttgttgct gagtagaaac ggcagataag
    41521 tcgaccattg tgatacctag agctggacgt gtcacttttc cgtctttttc cagttgattg
    41581 atgatattca ctacatcgtt acttggaata gcaaatccca ttccttctac gcttacatta
    41641 gaagtagatt cagaagtact tgcgatctta cttgagttga taccgataac ttgtccttcg
    41701 atattaacta aaggaccacc agaatttcca gggttgattg ctgcatctgt ttggatcgca
    41761 ttgatattga ctgtttcgtt cgattcattc gtacttgtca cttgtcggtt caaggaagaa
    41821 atgattcctg aagtgacaga atttgcatat tcagatccta gtggggaacc aatcgcaatc
    41881 gcaggctcac caaccttcag agcacttgag tcaccaaaag aggcaactgt ttcgactttg
    41941 tctgaattga tcttcaacac cgctaaatct gaataagcat ctgttccaac taattcagct
    42001 tttaccttcg taccgtcttt catcaagact tcaagacctt gctggccatc tactacgtga
    42061 ttattcgtga ctacataagc agtattcccg tcttttttgt agatcacgcc gcttccttca
    42121 ctataagctt ccaaattact atcatctgag ctgctacttt cttcttgctg cccgaatagt
    42181 ccgccaaaac cgcttgattg gttttgactt tgtaagttga taacagaaac gacagcgtct
    42241 tgaactttat ctaccgcttt ggtgatatca gaatctacat tgactttgac attttccaca
    42301 actgtttcac cggcagaatt ctgattgccg cttgtagcag tagaactatt gttgccagtg
    42361 cccatcgctg cataaaaaat gccagctgtc agtagtcctc caacgatccc tccaacaagc
    42421 cccagaccta acttacgcca gaggctatta tttttacttt ttttcatctt tggtgtcaca
    42481 ttttttctat ccatgtgttc ctcctccaat tacattcatt tatctactgt tagtatagtc
    42541 ttatttgttg actttagtct actttgagac tgtgaacttt ttatgaaaaa ctttaggaaa
    42601 aatcattgtt ttcagatgaa aatatttgat tttctttttc cacagatact gtgtaaaact
    42661 acgcggatag gattggaaaa ataagggata aaacatatcc acagcctgtg tataaagtgg
    42721 ataaattctt ataaaacgta tgtttgccta atgaatgtga tgaaataagg taaaataaca
    42781 ctgttttctg tggataaacc tgtggatgtt gtgcataact gttggggaaa tctttttaac
    42841 aaaaatagcc aaaataaaca tatagacgaa aaaattgatt ataataaaca aagtgagcat
    42901 gaagaccaaa ggagagaaaa catgaatatc aaaattatct cagtaggaaa attaaaagaa
    42961 aaatacctga tccaaggtat caatgagtat gtcaaacggc tgaatgccta tgcaaaaatc
    43021 gaattgatcg aagttcctga cgaaaaagcc ccggagaatt taagcgaagc gcaaatgaga
    43081 caagtaaaag aaaaagaagg ggaacggatc ttagcgaaaa tcaaagaaca agaatacgtc
    43141 tatgccttag caattgaagg gaaaaatcct acaagtgaat cttttgctaa acaaatcgac
    43201 caattaggta tccaaggaaa aagccacctt gtttttgtta tcggcggctc tttaggactc
    43261 agcgaagccg tcatgaaaag aagcaatgct cagatctctt ttggaaaaat gacgtatcca
    43321 catcaattga tgcgtctgat ccttgtcgag caaatctacc gtgccttccg gatcaatgcg
    43381 ggggctccat accacaaata atgatatttt ttaagagaaa aaattatccc tattttaaat
    43441 aatagcaata ggtagtggta gggagaaatt tataaataac ttttttattt gctttggtag
    43501 ccaagtaagt tgcaaaagaa aaatagatac tgttaaaaag ataatgtaga catagaaaca
    43561 aatacgtaaa attttagtta gaataatggc aaggagaaaa aacatgatag atgaaaaaaa
    43621 gtgtttaaaa aatctattag aggatatgga tgatattgaa gaaaactatc taaatggact
    43681 gggaaaacaa atatatttca gatacttaag ctatctaaaa atgaaataag acatagtaat
    43741 tttttagctt ttttgtttgc tccaaatgaa acccacaatt tatctgatga gttttttaaa
    43801 atgtttttga aaaggtatat agattctaat gatgatacca aagctgctat caattatttt
    43861 gatgctttac ttaatagcta tgaagattta attgtttaca gagaaaataa gaatattgat
    43921 attttattgg tatcggaaaa aaataaaata gtggtgtgca tcgaaaataa aattctgtct
    43981 tcggagtctg gagggcaact taataaatac caaatatata tagaacgcaa ttattctaac
    44041 tataaaaaaa tttttgtttt tttaactcca gatggaaatg aacccacaaa tcctagttgg
    44101 ggcataatta cttacaagaa tatcgttgaa gagagtgtaa aatattttgt gtaaatagaa
    44161 aaaaggaagt cccttctgta gaatagagtt accacaacac attcacagaa aagaggactt
    44221 ccatatgaac gattttacta cagaaattat ggaaatacta atcaataaag gtgatttgga
    44281 tgatttgttt cgtcgtcacc tagaattggc tattaacgct ttattacaag ctgaattaac
    44341 ggtatttctt gactatgaaa agtatgatag agctggattt aattcaggta attcccgtaa
    44401 tgggaattat tcgcgctctt ttaaaacaga gtacggtgaa ttgaatttag taatacctag
    44461 agataggaat ggagactttt ctcaacaaac tttacctgct tataaaagaa ccaatgactc
    44521 tttagaaacc actattattc agctagttca aaaaggaatt actatgtccg aaatctctga
    44581 tctaatcgaa aaaatgtatg gtcattatta caaaccacaa actatttcaa atatgagtaa
    44641 aattgtgtct gaagatgttt cggcatttaa agaaagaact ttagaagcta aatactcagt
    44701 catttttatg gatgctactc atattccctt aaaaagacaa accgtatcaa aagaagctgt
    44761 ctatatagta attggtatca ggttggatgg aactaaagag gttctaggat ttactatagc
    44821 tccaactgaa tctgcttatg tttggaaaga gattcttcaa gatctaaaag accgtggttt
    44881 agaagaggtt ttgttagtcg taacagatgg tttaagtggt attgacgata gtattcatag
    44941 tgtttatcca aatgctcaat ttcaacaatg ttgtgtccat atatctagaa atatcgctca
    45001 taaggttcgt gttagtgatc gacaagaaat ctgtagcga
//
LOCUS       DAYYFD010000009        44799 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000009 DAYYFD010000000
VERSION     DAYYFD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.009"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..795
                     /locus_tag="ACHA25_000472"
     CDS             <1..795
                     /locus_tag="ACHA25_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8761805.1"
                     /translation="ISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNLEAG
                     IVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVPLAV
                     AGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALLFIP
                     AQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAAISQ
                     LTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            816..1730
                     /locus_tag="ACHA25_000473"
     CDS             816..1730
                     /locus_tag="ACHA25_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8761806.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            1813..2187
                     /locus_tag="ACHA25_000474"
     CDS             1813..2187
                     /locus_tag="ACHA25_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8761807.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            2326..2571
                     /locus_tag="ACHA25_000475"
     CDS             2326..2571
                     /locus_tag="ACHA25_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8761808.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            2743..3807
                     /locus_tag="ACHA25_000476"
     CDS             2743..3807
                     /locus_tag="ACHA25_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8761809.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            4053..4478
                     /locus_tag="ACHA25_000477"
     CDS             4053..4478
                     /locus_tag="ACHA25_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8761810.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            4471..4920
                     /gene="nusB"
                     /locus_tag="ACHA25_000478"
     CDS             4471..4920
                     /gene="nusB"
                     /locus_tag="ACHA25_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8761811.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            5017..5871
                     /locus_tag="ACHA25_000479"
     CDS             5017..5871
                     /locus_tag="ACHA25_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8761812.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            5872..7209
                     /gene="xseA"
                     /locus_tag="ACHA25_000480"
     CDS             5872..7209
                     /gene="xseA"
                     /locus_tag="ACHA25_000480"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8761813.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            7214..7438
                     /locus_tag="ACHA25_000481"
     CDS             7214..7438
                     /locus_tag="ACHA25_000481"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8761814.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            7440..8330
                     /locus_tag="ACHA25_000482"
     CDS             7440..8330
                     /locus_tag="ACHA25_000482"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8761815.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            8339..9157
                     /locus_tag="ACHA25_000483"
     CDS             8339..9157
                     /locus_tag="ACHA25_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8761816.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            9264..9713
                     /locus_tag="ACHA25_000484"
     CDS             9264..9713
                     /locus_tag="ACHA25_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8761817.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            9731..11413
                     /gene="recN"
                     /locus_tag="ACHA25_000485"
     CDS             9731..11413
                     /gene="recN"
                     /locus_tag="ACHA25_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8761818.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(11468..11584)
                     /locus_tag="ACHA25_000486"
     CDS             complement(11468..11584)
                     /locus_tag="ACHA25_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8761819.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(11657..12613)
                     /locus_tag="ACHA25_000487"
     CDS             complement(11657..12613)
                     /locus_tag="ACHA25_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8761820.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            12784..13152
                     /locus_tag="ACHA25_000488"
     CDS             12784..13152
                     /locus_tag="ACHA25_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8761821.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            13322..13753
                     /gene="mraZ"
                     /locus_tag="ACHA25_000489"
     CDS             13322..13753
                     /gene="mraZ"
                     /locus_tag="ACHA25_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8761822.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            13774..14733
                     /gene="rsmH"
                     /locus_tag="ACHA25_000490"
     CDS             13774..14733
                     /gene="rsmH"
                     /locus_tag="ACHA25_000490"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8761823.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            14738..15139
                     /gene="ftsL"
                     /locus_tag="ACHA25_000491"
     CDS             14738..15139
                     /gene="ftsL"
                     /locus_tag="ACHA25_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8761824.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            15140..17332
                     /locus_tag="ACHA25_000492"
     CDS             15140..17332
                     /locus_tag="ACHA25_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8761825.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            17358..18320
                     /gene="mraY"
                     /locus_tag="ACHA25_000493"
     CDS             17358..18320
                     /gene="mraY"
                     /locus_tag="ACHA25_000493"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8761826.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            18334..19695
                     /gene="murD"
                     /locus_tag="ACHA25_000494"
     CDS             18334..19695
                     /gene="murD"
                     /locus_tag="ACHA25_000494"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8761827.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            19721..20809
                     /gene="murG"
                     /locus_tag="ACHA25_000495"
     CDS             19721..20809
                     /gene="murG"
                     /locus_tag="ACHA25_000495"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8761828.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            20824..22044
                     /locus_tag="ACHA25_000496"
     CDS             20824..22044
                     /locus_tag="ACHA25_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8761829.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            22200..23525
                     /gene="ftsA"
                     /locus_tag="ACHA25_000497"
     CDS             22200..23525
                     /gene="ftsA"
                     /locus_tag="ACHA25_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8761830.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            23548..24789
                     /gene="ftsZ"
                     /locus_tag="ACHA25_000498"
     CDS             23548..24789
                     /gene="ftsZ"
                     /locus_tag="ACHA25_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8761831.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            24806..25483
                     /locus_tag="ACHA25_000499"
     CDS             24806..25483
                     /locus_tag="ACHA25_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8761832.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            25497..26096
                     /locus_tag="ACHA25_000500"
     CDS             25497..26096
                     /locus_tag="ACHA25_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8761833.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            26177..26398
                     /locus_tag="ACHA25_000501"
     CDS             26177..26398
                     /locus_tag="ACHA25_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8761834.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            26447..27229
                     /locus_tag="ACHA25_000502"
     CDS             26447..27229
                     /locus_tag="ACHA25_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8761835.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            27250..27957
                     /locus_tag="ACHA25_000503"
     CDS             27250..27957
                     /locus_tag="ACHA25_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8761836.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            28219..31017
                     /gene="ileS"
                     /locus_tag="ACHA25_000504"
     CDS             28219..31017
                     /gene="ileS"
                     /locus_tag="ACHA25_000504"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8761837.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(31066..32592)
                     /gene="zwf"
                     /locus_tag="ACHA25_000505"
     CDS             complement(31066..32592)
                     /gene="zwf"
                     /locus_tag="ACHA25_000505"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8761838.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(32607..33254)
                     /locus_tag="ACHA25_000506"
     CDS             complement(32607..33254)
                     /locus_tag="ACHA25_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8761839.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(33438..33767)
                     /locus_tag="ACHA25_000507"
     CDS             complement(33438..33767)
                     /locus_tag="ACHA25_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8761840.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            33944..34672
                     /gene="sfsA"
                     /locus_tag="ACHA25_000508"
     CDS             33944..34672
                     /gene="sfsA"
                     /locus_tag="ACHA25_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8761841.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            34688..35701
                     /locus_tag="ACHA25_000509"
     CDS             34688..35701
                     /locus_tag="ACHA25_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8761842.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            35701..36978
                     /locus_tag="ACHA25_000510"
     CDS             35701..36978
                     /locus_tag="ACHA25_000510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8761843.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            37041..39743
                     /locus_tag="ACHA25_000511"
     CDS             37041..39743
                     /locus_tag="ACHA25_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8761844.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            39895..40212
                     /locus_tag="ACHA25_000512"
     CDS             39895..40212
                     /locus_tag="ACHA25_000512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8761845.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            40242..40562
                     /locus_tag="ACHA25_000513"
     CDS             40242..40562
                     /locus_tag="ACHA25_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8761846.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            40670..42130
                     /locus_tag="ACHA25_000514"
     CDS             40670..42130
                     /locus_tag="ACHA25_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8761847.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            42198..42419
                     /locus_tag="ACHA25_000515"
     CDS             42198..42419
                     /locus_tag="ACHA25_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761848.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            42450..42632
                     /locus_tag="ACHA25_000516"
     CDS             42450..42632
                     /locus_tag="ACHA25_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8761849.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            42632..43045
                     /locus_tag="ACHA25_000517"
     CDS             42632..43045
                     /locus_tag="ACHA25_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8761850.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            43168..44349
                     /locus_tag="ACHA25_000518"
     CDS             43168..44349
                     /locus_tag="ACHA25_000518"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8761851.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(44420..44584)
                     /locus_tag="ACHA25_000519"
     CDS             complement(44420..44584)
                     /locus_tag="ACHA25_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761852.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
BASE COUNT        14872 a         7595 c         9676 g        12656 t
ORIGIN      
        1 atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat
       61 gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac
      121 ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat
      181 atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta
      241 gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg
      301 gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat
      361 ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt
      421 gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca
      481 gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct
      541 atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca
      601 aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca
      661 ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa
      721 atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt
      781 ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat
      841 caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact
      901 acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat
      961 acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac
     1021 acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga
     1081 atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt
     1141 tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag
     1201 gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga
     1261 acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta
     1321 aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt
     1381 tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag
     1441 ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag
     1501 gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg
     1561 aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta gctgcattag
     1621 gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc
     1681 ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa
     1741 tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc
     1801 tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact
     1861 tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtaataatgg ttttgagttt
     1921 taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta
     1981 ctagcatcag tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa
     2041 aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa
     2101 tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt
     2161 attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct
     2221 ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt
     2281 aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg
     2341 acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat
     2401 tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg
     2461 attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc
     2521 gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca
     2581 aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt
     2641 aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg
     2701 ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa
     2761 aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac
     2821 aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa
     2881 aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg
     2941 tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa
     3001 gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt
     3061 ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga
     3121 gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg
     3181 gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat
     3241 caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt
     3301 gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa
     3361 ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca
     3421 cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta
     3481 gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat
     3541 gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca
     3601 ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag
     3661 caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt
     3721 ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt
     3781 tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca
     3841 gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt
     3901 ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg
     3961 atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta
     4021 tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa
     4081 acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg
     4141 ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg
     4201 tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag
     4261 atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag
     4321 caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat
     4381 ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg
     4441 aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt
     4501 cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa
     4561 gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca
     4621 gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa
     4681 ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa
     4741 atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca
     4801 gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca
     4861 cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa
     4921 gtcctagctt ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt
     4981 agaatgaaaa cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa
     5041 gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat
     5101 ggtatccatc ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat
     5161 gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat
     5221 cctgagacga tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct
     5281 agttttcatg gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc
     5341 ctgttagcta tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga
     5401 ctccttgcgg gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt
     5461 gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc
     5521 gtcggtaaac cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat
     5581 tctaaaaccg aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata
     5641 ggacgtggac attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta
     5701 gggatgaatc gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag
     5761 cctattgcag gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg
     5821 ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa
     5881 gaatatttaa ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct
     5941 tatttagaac gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc
     6001 catcaatatt tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga
     6061 gcattccaaa aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt
     6121 atttcattat atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat
     6181 ggaataggtg ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc
     6241 ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc
     6301 agtccaagcg gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt
     6361 gtccaattgg ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc
     6421 aggaatatcc agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg
     6481 ggtggttcga ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat
     6541 caagctaata cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat
     6601 ttagtagcag atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt
     6661 ttgtcagaag agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta
     6721 cgccagatcc agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg
     6781 cagcctgaaa gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta
     6841 tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac
     6901 caattggaac aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat
     6961 ctagaaaaac gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt
     7021 gctcaagctg ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac
     7081 agctatacga cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat
     7141 ttgctgacga tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa
     7201 ggagaataaa aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat
     7261 tgtcatgcag ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg
     7321 aggaatggaa ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac
     7381 gaaaatgatg acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga
     7441 tgagattaac tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt
     7501 ttttagatga gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag
     7561 caggcgggaa aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc
     7621 ctattgatgt tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct
     7681 tgatccacga tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca
     7741 atcataaagt attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag
     7801 cattccagct aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc
     7861 aattagctgt ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg
     7921 gcgaatcgaa aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc
     7981 atttgattcg ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct
     8041 tattgctact tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc
     8101 tactcgatgt gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta
     8161 tggaaaaaaa tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga
     8221 ttgagctgtt atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg
     8281 atggcgggct gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat
     8341 ggaaaaagaa cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca
     8401 agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa
     8461 accgggagaa aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta
     8521 tgtttctcga gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga
     8581 aggaaaaaca ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca
     8641 aaatggcgca aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat
     8701 aagacaagat cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga
     8761 agactttacc ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa
     8821 actgatgctg cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat
     8881 caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc
     8941 tcagacccat caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga
     9001 tgtgctggaa ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc
     9061 tcacttgagg aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt
     9121 ggtttcaaat gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg
     9181 agaagtatgc ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga
     9241 atatgctaag gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat
     9301 tgttgagtga gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag
     9361 gaatatctgt tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg
     9421 taccggcttc agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa
     9481 atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat
     9541 ttgttatttt gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac
     9601 gttacaagaa agaattattt tcgattatca atgatgacga taatgttttg atgattacac
     9661 gaactgaaga ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg
     9721 agcgaatgtg atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg
     9781 gctttctttt cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat
     9841 tattatcgat gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca
     9901 aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc
     9961 tgaactgatg gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga
    10021 tatgtcctta actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa
    10081 tctgagaaga atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact
    10141 gctccagcct gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca
    10201 gaagaaaaag agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg
    10261 aaaagtgcag caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca
    10321 agaagaaata gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag
    10381 agaaaaactt agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact
    10441 gacgaataat gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg
    10501 gatcgcccat ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta
    10561 tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga
    10621 gagtcgttta gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata
    10681 cggcgaatcc attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc
    10741 ttctgatttt tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct
    10801 tctacttcag caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt
    10861 ggaaaaatcc attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt
    10921 acgtttctta aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt
    10981 ttacattacg accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg
    11041 cgaactgtct cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac
    11101 aagtattatt tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc
    11161 agataaaatt tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca
    11221 agttgctgca gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac
    11281 gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct
    11341 ggctggaagt gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc
    11401 aaaaaggacc tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat
    11461 tttattttta catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca
    11521 gcattgtcca aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac
    11581 gcattgtttt caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt
    11641 ccacaagagg gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac
    11701 aaataaataa tgttaataga actagccata taaaggcatc atccctttca gcaataggta
    11761 acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta
    11821 aagaagttaa aattttcata atattattca gattattcga tacgattgct gaaaaagtct
    11881 gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt
    11941 ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt
    12001 gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc
    12061 ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg
    12121 cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga
    12181 cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg
    12241 tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct
    12301 ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac
    12361 ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat
    12421 tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg
    12481 gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat
    12541 accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa
    12601 aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat
    12661 tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat
    12721 gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg
    12781 ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg
    12841 tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc
    12901 cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg
    12961 acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct
    13021 gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg
    13081 cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt
    13141 tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct
    13201 tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt
    13261 ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc
    13321 aatgttgatg ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc
    13381 aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct
    13441 tttcggctat ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt
    13501 ggctaaaaaa gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga
    13561 acttgataaa caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac
    13621 aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg
    13681 gcaagatttt tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga
    13741 tttcggacta taagaatagg aagaggctga ctaatgactg aaacatttca acactataca
    13801 gtcatgttga aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac
    13861 tgtacactcg gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg
    13921 catctgtatg cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct
    13981 tcttatgtcg aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa
    14041 cgcttgagtg agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct
    14101 cctcagctag atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg
    14161 cgcatggatc agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat
    14221 gaattagtaa agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt
    14281 gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc
    14341 attaaacaag tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt
    14401 tttcaagctg tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa
    14461 caggcaatcc ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg
    14521 gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg
    14581 ggacttcctg tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca
    14641 atccttcctt cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt
    14701 attgcagaaa aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca
    14761 agattacgaa tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc
    14821 ttcttcgaat caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc
    14881 tgctttggaa aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt
    14941 aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac
    15001 agaaatcaat gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc
    15061 tcgtactgaa cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa
    15121 tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa
    15181 aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat
    15241 tggattgttc tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc
    15301 tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc
    15361 aaaaagagga acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta
    15421 ttcaattaaa gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga
    15481 agaaaagaat tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa
    15541 agatgcactg aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg
    15601 tagctatggg aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa
    15661 agaaggtgtc gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt
    15721 cttttcttct cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg
    15781 taaactaggt ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat
    15841 ttatcaaaaa gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc
    15901 tgtcgacgga caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac
    15961 actgatggat caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa
    16021 agcaaaaacc ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat
    16081 ggaaggattg actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga
    16141 accaggatca accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa
    16201 cgagaatgaa accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca
    16261 tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt
    16321 cgggatggtc atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact
    16381 gggatttgga caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac
    16441 attaaatatt gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt
    16501 ccaaatgatg aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata
    16561 tatcagcaaa gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct
    16621 agggcaacct ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt
    16681 cgaaagcgaa aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc
    16741 ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac
    16801 agcctacctg tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta
    16861 tctaacaatg aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc
    16921 attgatgaaa cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa
    16981 aacagaaaaa gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga
    17041 tgctcaaaaa agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca
    17101 atctactgcc aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg
    17161 tgataagctg atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa
    17221 aatcctcggt attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc
    17281 tccatatgaa aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa
    17341 ataggagaga aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg
    17401 caatgacaat tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc
    17461 aggcgattcg tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg
    17521 gcggcttagt gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa
    17581 aacagctaac tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg
    17641 gctttcttga tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc
    17701 agaaattgat tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag
    17761 gttatccagg tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta
    17821 ttttcgctct cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg
    17881 atggattagt tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc
    17941 atcagcatca gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt
    18001 tcttcgtcta caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt
    18061 tgggcggact tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga
    18121 ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc
    18181 ttacaggaaa acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat
    18241 ggtcggagtg gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca
    18301 cattatggat cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat
    18361 aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat
    18421 gaattgggcg cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca
    18481 caagagctgc tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg
    18541 gatgaagaat tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta
    18601 gcaaaagccc aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta
    18661 gcggaatgcc caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg
    18721 acaggactac tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt
    18781 gggtatccag caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa
    18841 ttgtctagct tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc
    18901 aatatctatg aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa
    18961 tggaaccttc aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca
    19021 gaactacaag aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa
    19081 gtgttggaag acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata
    19141 atggaaatat ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct
    19201 atttctgtag caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt
    19261 ttccatggcg taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat
    19321 aatgattcaa aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg
    19381 tctaaggtcg tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt
    19441 ccttcattaa aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg
    19501 gatgctggaa aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca
    19561 gttcctttag cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat
    19621 gccagctggg atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg
    19681 aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg
    19741 cggaggaaca gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca
    19801 agaaccgaat gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt
    19861 ccctgataca ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc
    19921 catgcataac atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat
    19981 cctcagtgat tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt
    20041 agtgtacgct gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc
    20101 aggtgttacc aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga
    20161 tgcggcttcc ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga
    20221 agtagcagat actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa
    20281 aacagtcttg atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt
    20341 ttttttaagt gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata
    20401 ttatgaagag atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta
    20461 tatcaaaaat atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc
    20521 aacttcgatc gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata
    20581 tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa
    20641 aatgattgca gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat
    20701 gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga
    20761 tgcttcaaaa cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc
    20821 ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat
    20881 ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa
    20941 gagccaaaaa aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa
    21001 acggttcaag aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa
    21061 gagatcaagc aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc
    21121 aatttaaaaa gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta
    21181 ttgggtatac cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca
    21241 gttgcagtat caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg
    21301 tcattaggag aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt
    21361 aaaaaagcac agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc
    21421 gatttggatg taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac
    21481 ccagtgattg aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc
    21541 cctatcttgg aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat
    21601 cagttatcat cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc
    21661 aataaaaatc tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat
    21721 aatcttgcga accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt
    21781 gtgatcgaca tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa
    21841 gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa
    21901 tcaagtgaag aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga
    21961 tcatctgatt caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact
    22021 ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa
    22081 aaaccagtaa tttatggtag aattggaagc agtgaatata tctattaata agtatatttt
    22141 aaaacggacg ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca
    22201 tggcaaaaac aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg
    22261 tggctgaata tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag
    22321 ggattaaccg aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag
    22381 tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag
    22441 caaacatgct tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg
    22501 aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc
    22561 ctgaacgtca aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa
    22621 tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag
    22681 gacctaaaac gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca
    22741 acgaaatggt gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag
    22801 attttggtac gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg
    22861 ataagcaatt gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata
    22921 tctctattgt attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg
    22981 atgcataccc agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt
    23041 cagaaccagt gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac
    23101 agattttcaa caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg
    23161 gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa
    23221 tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat
    23281 ttacaaatgt aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg
    23341 caaaaattgc tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa
    23401 caacaaacac atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac
    23461 caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg
    23521 attaattcat cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac
    23581 ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg
    23641 attgaagaaa acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta
    23701 aaaaattcaa aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt
    23761 gctggttctc agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc
    23821 gaagctttag acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt
    23881 acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt
    23941 gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt
    24001 atcgctcgtt tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta
    24061 gaagttgtag ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta
    24121 cgacaaggtg tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac
    24181 tttgctgacg tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg
    24241 gctagtggag aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt
    24301 gaaacatcta ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg
    24361 acattgttcg aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta
    24421 aatatcattc taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc
    24481 atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa
    24541 gcacaaatgc aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact
    24601 tcttcagaag aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt
    24661 cctagagttg atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac
    24721 cgcgaagaaa caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt
    24781 aaaaaataaa ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga
    24841 aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt
    24901 aacaaaatct gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg
    24961 tgcagaaaac cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga
    25021 aataaagtgg catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga
    25081 aattgattac tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt
    25141 agagaaagaa ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg
    25201 aatttcacca gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa
    25261 ggttgttggg ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt
    25321 gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc
    25381 atgtagtgaa ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc
    25441 aacatttatc cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc
    25501 cgcttttaaa cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg
    25561 aataccaaga ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa
    25621 cacagaatca atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg
    25681 cgacttcacg tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg
    25741 aactacatca atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta
    25801 cacaacctaa agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg
    25861 aagctatggc gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt
    25921 tagtagaaga aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac
    25981 tagatggaga tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg
    26041 agattgacag ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg
    26101 aggaactacg atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt
    26161 ctatagcatt atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc
    26221 agggctagga cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct
    26281 gcctttgagg attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt
    26341 agccgtacaa ggtttagcac agatcgttat atggataact gtccatttag tttattgatt
    26401 gaagcaacaa aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt
    26461 ataccagcac tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga
    26521 acaggttcaa ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta
    26581 tattttagaa acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata
    26641 tgaacaggca gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga
    26701 agattttgaa acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca
    26761 tgggaaaatc cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat
    26821 catctcggac ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc
    26881 tgcacaagta acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga
    26941 aatgattatg ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact
    27001 agacagtgtg atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga
    27061 agctggtaaa gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact
    27121 attagatatt atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa
    27181 gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt
    27241 ggtttactca tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg
    27301 cggggatata atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag
    27361 gaattgactc aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt
    27421 gagtacttca atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg
    27481 gctgataaag taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa
    27541 aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat
    27601 gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg
    27661 acagaaactg aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg
    27721 cttgagtctc aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc
    27781 tccagctacg tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac
    27841 aatgaaaatg atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca
    27901 tttgatacac aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac
    27961 agaaaagcac tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact
    28021 gcatggtgaa agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg
    28081 gctgacctcg ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg
    28141 gtaccacggc atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat
    28201 aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc
    28261 cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat
    28321 cgaatctacc aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat
    28381 ggacctccat atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa
    28441 gacattatca ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg
    28501 gatacacatg gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa
    28561 atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag
    28621 caacgagcag atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca
    28681 ttagatcctt cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc
    28741 tatatttaca aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg
    28801 gaagctgaaa ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc
    28861 aaagacggca aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca
    28921 tggacattgc cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg
    28981 gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa
    29041 atcggttggg aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg
    29101 actgcaagtc atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact
    29161 ttagacgctg gtactggatt agtccatact gctccaggac atggggaaga tgactatatc
    29221 gctggtagca aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca
    29281 gaagaagcac ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat
    29341 ctattaaaag aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac
    29401 gattggcgga cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc
    29461 gataaattcc gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg
    29521 ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag
    29581 cgtgcatggg gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca
    29641 ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt
    29701 gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc
    29761 gaatttacaa aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa
    29821 gctgttttac gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca
    29881 gaccaatatc gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta
    29941 gcaccataca aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg
    30001 agtaaatctt taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat
    30061 atccttcgct tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat
    30121 atcttaagtc aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta
    30181 gcgaatacag aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct
    30241 gtagacaaat atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg
    30301 tacgacaaat ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat
    30361 ctatcgtcat tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat
    30421 tatcaacgtc gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta
    30481 ttgactccta tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag
    30541 gagtacgttc agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg
    30601 atggatacct ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa
    30661 gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa
    30721 caacaagttc gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt
    30781 tcaccagact actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta
    30841 tttgatgatg ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc
    30901 caagttcgta aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca
    30961 aaaatcgttg aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag
    31021 ggaaaagggg gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt
    31081 tccacgttca cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag
    31141 tagttcgaaa gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg
    31201 tgtttggtcc caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc
    31261 ccaatgagag aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc
    31321 ttctgggcta ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt
    31381 ttcaaatcct tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg
    31441 aatatagatc gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga
    31501 tgttttgaat acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc
    31561 agtcaaacgt ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat
    31621 agtgaatttg cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc
    31681 gcgatagccc ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa
    31741 gttttctaac acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc
    31801 tgtacggatt tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac
    31861 ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc
    31921 gcgatcttct acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt
    31981 gttccattga gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat
    32041 ttcttttcct aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat
    32101 ttgctggttc aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa
    32161 acgattgaag ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc
    32221 aaaaaattga ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc
    32281 atctaatcgg tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg
    32341 ggattgatag taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc
    32401 acggattgtt tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt
    32461 tccgattaca gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg
    32521 gtacaattta cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt
    32581 ttcattagac atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc
    32641 ttaaagctga cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct
    32701 gcgatgtcaa cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc
    32761 aacaattctt tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac
    32821 tccaatgttt gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca
    32881 tgaggacaat gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa
    32941 gtcacatgtt ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt
    33001 tcaactaaaa aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt
    33061 ccgcttgcag tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca
    33121 agtttagaaa tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt
    33181 ttattgttga ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct
    33241 cgatttggtg tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa
    33301 caaaaaaaac gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt
    33361 tacaacaaaa aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata
    33421 tatacattgg gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg
    33481 tcataaagag tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg
    33541 actaaacgat attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa
    33601 aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat
    33661 gtaccttgat actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa
    33721 ataaaacttg agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc
    33781 aaatcactcc tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg
    33841 taccgaataa aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta
    33901 caaattatga taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt
    33961 ggaaatagct ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga
    34021 cggagaaata attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc
    34081 aggcgctgaa gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct
    34141 gattgctgta aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct
    34201 ggcaattgat ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac
    34261 ggtatacaag cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt
    34321 ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg
    34381 tgcttttcct gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc
    34441 cgcacaagat ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct
    34501 ggcaacgatc catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca
    34561 aagcggagtt caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa
    34621 gcaggccatt ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg
    34681 taaaataatg atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca
    34741 agcagcttta agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa
    34801 agcacaggaa tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac
    34861 ttttttcaat attactcata tggacaccat ttatattgct tctccgaata gtcttcactt
    34921 tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt
    34981 ttcgacgcca aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt
    35041 ttttgaagca gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc
    35101 attaaaagac gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga
    35161 ccaagtatta gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt
    35221 gatggattta ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga
    35281 agtccattat ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt
    35341 acgttatgag aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc
    35401 gtcagagatt tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca
    35461 agcacaattt catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga
    35521 aaatccaatg gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc
    35581 agcttgggga ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat
    35641 ttatgattta agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta
    35701 atgaacgaat ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg
    35761 tttatctcac cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt
    35821 atagtagcgg tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta
    35881 ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta
    35941 gctattcaag tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa
    36001 tctttggtgg gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa
    36061 atcttgattg gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca
    36121 catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc
    36181 caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca
    36241 gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca
    36301 attgacgtga cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta
    36361 ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt
    36421 ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga
    36481 ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta
    36541 gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt
    36601 ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct
    36661 tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc
    36721 gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt
    36781 gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca
    36841 gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa
    36901 tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa
    36961 ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg
    37021 gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag
    37081 aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca
    37141 atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc
    37201 gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact
    37261 cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca
    37321 tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg
    37381 gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt
    37441 cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa
    37501 atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag
    37561 ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga
    37621 tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt
    37681 accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga
    37741 cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca
    37801 ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta
    37861 tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg
    37921 ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag
    37981 tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa
    38041 aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac
    38101 gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg
    38161 atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa
    38221 aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa
    38281 aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca
    38341 aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa
    38401 aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa
    38461 gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac
    38521 ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag
    38581 aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca
    38641 atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg
    38701 gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag
    38761 ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac
    38821 aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg
    38881 gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta
    38941 tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa
    39001 gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt
    39061 tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta
    39121 ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata
    39181 aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg
    39241 ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa
    39301 atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc
    39361 gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa
    39421 gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc
    39481 agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag
    39541 ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta
    39601 ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag
    39661 aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc
    39721 ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt
    39781 gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat
    39841 tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca
    39901 aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa
    39961 atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca
    40021 gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta
    40081 cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc
    40141 atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat
    40201 ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc
    40261 tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag
    40321 cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag
    40381 aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag
    40441 gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta
    40501 ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat
    40561 aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt
    40621 tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact
    40681 atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca
    40741 tttagttgct ttgcgtgatg cttttatcgg catactgccc gcaacaatgg cgggatcagt
    40801 cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc
    40861 tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa
    40921 tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg
    40981 gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc
    41041 taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt
    41101 tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg
    41161 cacactgtca gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta
    41221 ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc
    41281 caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc
    41341 cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg
    41401 tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct
    41461 aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg
    41521 gttctttggt attcatggat caaatgtatt ggcaccagtg ctagagggaa tctggggaca
    41581 agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc
    41641 agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga
    41701 tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat
    41761 tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt
    41821 caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat
    41881 cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg
    41941 cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt
    42001 cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac
    42061 tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga
    42121 acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt
    42181 ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag
    42241 aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa
    42301 tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg
    42361 aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga
    42421 aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta
    42481 ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata
    42541 gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa
    42601 aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc
    42661 agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac
    42721 cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca
    42781 aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg
    42841 tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc
    42901 gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa
    42961 tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct
    43021 agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa
    43081 atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact
    43141 agcttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct
    43201 tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa
    43261 aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag
    43321 gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa
    43381 gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc
    43441 gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta
    43501 tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt
    43561 gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga
    43621 aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag
    43681 atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc
    43741 aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga
    43801 tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat
    43861 catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca
    43921 tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt
    43981 gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg
    44041 gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat
    44101 tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc
    44161 tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag
    44221 ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca
    44281 aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac
    44341 tatttttgat aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt
    44401 tggcatttga aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt
    44461 tttcttttgt tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt
    44521 cccgattttt tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat
    44581 tcattggaaa ggacctccct acattatttc attctacctc tagtttatca tctgttaagg
    44641 actagtggaa acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag
    44701 taaaaaacaa acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag
    44761 agcaatcgcc tcctaagcga taggtcgctg gttcaattc
//
LOCUS       DAYYFD010000010        41707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000010 DAYYFD010000000
VERSION     DAYYFD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.010"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA25_000520"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA25_000520"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8761853.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA25_000521"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA25_000521"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8761854.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA25_000522"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA25_000522"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8761855.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHA25_000523"
     CDS             2179..2499
                     /locus_tag="ACHA25_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8761856.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACHA25_000524"
     CDS             complement(2611..3468)
                     /locus_tag="ACHA25_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8761857.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHA25_000525"
     CDS             complement(3576..4880)
                     /locus_tag="ACHA25_000525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8761858.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHA25_000526"
     CDS             complement(4877..5386)
                     /locus_tag="ACHA25_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761859.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHA25_000527"
     CDS             complement(5561..7696)
                     /locus_tag="ACHA25_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8761860.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHA25_000528"
     CDS             complement(7779..9434)
                     /locus_tag="ACHA25_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8761861.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHA25_000529"
     CDS             complement(9436..9750)
                     /locus_tag="ACHA25_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8761862.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHA25_000530"
     CDS             9920..10180
                     /locus_tag="ACHA25_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8761863.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHA25_000531"
     CDS             10194..10508
                     /locus_tag="ACHA25_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8761864.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHA25_000532"
     CDS             complement(11238..11792)
                     /locus_tag="ACHA25_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8761865.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHA25_000533"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHA25_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHA25_000534"
     CDS             complement(14153..14869)
                     /locus_tag="ACHA25_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8761866.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA25_000535"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA25_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8761867.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA25_000536"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA25_000536"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8761868.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHA25_000537"
     CDS             complement(18339..19799)
                     /locus_tag="ACHA25_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8761869.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHA25_000538"
     CDS             complement(19789..21084)
                     /locus_tag="ACHA25_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8761870.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHA25_000539"
     CDS             complement(21416..22093)
                     /locus_tag="ACHA25_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8761871.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHA25_000540"
     CDS             complement(22123..23214)
                     /locus_tag="ACHA25_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8761872.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHA25_000541"
     CDS             complement(23315..23749)
                     /locus_tag="ACHA25_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8761873.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHA25_000542"
     CDS             complement(24333..25790)
                     /locus_tag="ACHA25_000542"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8761874.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHA25_000543"
     CDS             complement(25787..27682)
                     /locus_tag="ACHA25_000543"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8761875.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHA25_000544"
     CDS             complement(27740..29515)
                     /locus_tag="ACHA25_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8761876.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHA25_000545"
     CDS             complement(29565..31061)
                     /locus_tag="ACHA25_000545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8761877.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHA25_000546"
     CDS             complement(31255..32877)
                     /locus_tag="ACHA25_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8761878.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHA25_000547"
     CDS             complement(33205..34518)
                     /locus_tag="ACHA25_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8761879.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACHA25_000548"
     CDS             complement(34763..34987)
                     /locus_tag="ACHA25_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761880.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACHA25_000549"
     CDS             complement(35007..35429)
                     /locus_tag="ACHA25_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8761881.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACHA25_000550"
     CDS             complement(35571..36281)
                     /locus_tag="ACHA25_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8761882.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACHA25_000551"
     CDS             complement(36299..36796)
                     /locus_tag="ACHA25_000551"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8761883.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACHA25_000552"
     CDS             complement(36833..37285)
                     /locus_tag="ACHA25_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8761884.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="ACHA25_000553"
     CDS             complement(37420..38025)
                     /locus_tag="ACHA25_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8761885.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="ACHA25_000554"
     CDS             complement(38475..39095)
                     /locus_tag="ACHA25_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8761886.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="ACHA25_000555"
     CDS             complement(39097..39966)
                     /locus_tag="ACHA25_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8761887.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="ACHA25_000556"
     CDS             complement(39989..40396)
                     /locus_tag="ACHA25_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8761888.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="ACHA25_000557"
     CDS             complement(40415..41704)
                     /locus_tag="ACHA25_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8761889.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8982 c         7022 g        13944 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatta catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgtt
//
LOCUS       DAYYFD010000011        41009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000011 DAYYFD010000000
VERSION     DAYYFD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.011"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1364
                     /locus_tag="ACHA25_000558"
     CDS             24..1364
                     /locus_tag="ACHA25_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8761890.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1582..2025
                     /locus_tag="ACHA25_000559"
     CDS             1582..2025
                     /locus_tag="ACHA25_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8761891.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2529..3947)
                     /gene="nhaC"
                     /locus_tag="ACHA25_000560"
     CDS             complement(2529..3947)
                     /gene="nhaC"
                     /locus_tag="ACHA25_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8761892.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4350..5573)
                     /locus_tag="ACHA25_000561"
     CDS             complement(4350..5573)
                     /locus_tag="ACHA25_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8761893.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5604..6443)
                     /locus_tag="ACHA25_000562"
     CDS             complement(5604..6443)
                     /locus_tag="ACHA25_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8761894.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6460..7140)
                     /locus_tag="ACHA25_000563"
     CDS             complement(6460..7140)
                     /locus_tag="ACHA25_000563"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8761895.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7142..8215)
                     /locus_tag="ACHA25_000564"
     CDS             complement(7142..8215)
                     /locus_tag="ACHA25_000564"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8761896.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWMVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8271..8509)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8552..8785)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8862..9533)
                     /locus_tag="ACHA25_000565"
     CDS             complement(8862..9533)
                     /locus_tag="ACHA25_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8761897.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9620..12268)
                     /gene="mgtA"
                     /locus_tag="ACHA25_000566"
     CDS             complement(9620..12268)
                     /gene="mgtA"
                     /locus_tag="ACHA25_000566"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8761898.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12610..12784)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12957..13394
                     /gene="ndk"
                     /locus_tag="ACHA25_000567"
     CDS             12957..13394
                     /gene="ndk"
                     /locus_tag="ACHA25_000567"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8761899.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13416..14282)
                     /locus_tag="ACHA25_000568"
     CDS             complement(13416..14282)
                     /locus_tag="ACHA25_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8761900.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14442..16535)
                     /locus_tag="ACHA25_000569"
     CDS             complement(14442..16535)
                     /locus_tag="ACHA25_000569"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8761901.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17284..17907)
                     /locus_tag="ACHA25_000570"
     CDS             complement(17284..17907)
                     /locus_tag="ACHA25_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761902.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18066..19463)
                     /locus_tag="ACHA25_000571"
     CDS             complement(18066..19463)
                     /locus_tag="ACHA25_000571"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8761903.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19495..20466)
                     /locus_tag="ACHA25_000572"
     CDS             complement(19495..20466)
                     /locus_tag="ACHA25_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8761904.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20840..21265
                     /locus_tag="ACHA25_000573"
     CDS             20840..21265
                     /locus_tag="ACHA25_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761905.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21753..22454)
                     /locus_tag="ACHA25_000574"
     CDS             complement(21753..22454)
                     /locus_tag="ACHA25_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8761906.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22470..22910)
                     /locus_tag="ACHA25_000575"
     CDS             complement(22470..22910)
                     /locus_tag="ACHA25_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8761907.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22910..23485)
                     /locus_tag="ACHA25_000576"
     CDS             complement(22910..23485)
                     /locus_tag="ACHA25_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8761908.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            23726..23935
                     /locus_tag="ACHA25_000577"
     CDS             23726..23935
                     /locus_tag="ACHA25_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8761909.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(24333..24845)
                     /locus_tag="ACHA25_000578"
                     /pseudo
     CDS             complement(24333..24845)
                     /locus_tag="ACHA25_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(24971..25672)
                     /locus_tag="ACHA25_000579"
     CDS             complement(24971..25672)
                     /locus_tag="ACHA25_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8761910.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25780..27223)
                     /locus_tag="ACHA25_000580"
                     /pseudo
     CDS             complement(25780..27223)
                     /locus_tag="ACHA25_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(27237..29102)
                     /locus_tag="ACHA25_000581"
     CDS             complement(27237..29102)
                     /locus_tag="ACHA25_000581"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8761911.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29223..30073)
                     /gene="licT"
                     /locus_tag="ACHA25_000582"
                     /pseudo
     CDS             complement(29223..30073)
                     /gene="licT"
                     /locus_tag="ACHA25_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            complement(30383..31096)
                     /locus_tag="ACHA25_000583"
     CDS             complement(30383..31096)
                     /locus_tag="ACHA25_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8761912.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31164..31490)
                     /locus_tag="ACHA25_000584"
     CDS             complement(31164..31490)
                     /locus_tag="ACHA25_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8761913.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31613..31840)
                     /locus_tag="ACHA25_000585"
     CDS             complement(31613..31840)
                     /locus_tag="ACHA25_000585"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8761914.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(32162..33415)
                     /locus_tag="ACHA25_000586"
     CDS             complement(32162..33415)
                     /locus_tag="ACHA25_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8761915.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            33476..34216
                     /locus_tag="ACHA25_000587"
     CDS             33476..34216
                     /locus_tag="ACHA25_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8761916.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(34302..35060)
                     /locus_tag="ACHA25_000588"
     CDS             complement(34302..35060)
                     /locus_tag="ACHA25_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8761917.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(35057..35521)
                     /locus_tag="ACHA25_000589"
                     /pseudo
     CDS             complement(35057..35521)
                     /locus_tag="ACHA25_000589"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(35540..36037)
                     /locus_tag="ACHA25_000590"
     CDS             complement(35540..36037)
                     /locus_tag="ACHA25_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8761918.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(36105..39272)
                     /locus_tag="ACHA25_000591"
     CDS             complement(36105..39272)
                     /locus_tag="ACHA25_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8761919.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(39496..40380)
                     /locus_tag="ACHA25_000592"
     CDS             complement(39496..40380)
                     /locus_tag="ACHA25_000592"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8761920.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(40475..40912)
                     /locus_tag="ACHA25_000593"
     CDS             complement(40475..40912)
                     /locus_tag="ACHA25_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761921.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        11733 a         8629 c         6981 g        13666 t
ORIGIN      
        1 aatttaaaag aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa cttagctcca
       61 gacatatcac catgttagct ttaggtggag caatcggtgc aggtctattt aaaggaagtg
      121 gcgaagcgat tgggatcgcc ggcccttctg tattgattgc ttttttgatt ggtgggctga
      181 ttttatatat cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc
      241 acggattatc tggtttgata cgcccttatc taggtgctca ttctgctgat tttactgatt
      301 gggtctactg gtctatgtgg attatcaata tcatcgctga agctgttgca gctgcttcct
      361 ttttgcagtt gtggttcccg cacattccag cctgggtatt tgtttttatg ttggctgttt
      421 taactacgat catcaatctt tattcggtac ggttattcgc agaaacagaa tattggctgg
      481 cttttgctaa aatcagtgtc attatcttac tgatcatttt tgctacttat cttgtcggtc
      541 agcaaatgtt gggcacaggc gttttcccta cccttcagca attgactgac catggtgggt
      601 ttactcctca tggtatgaag ggaatcatca gttctttgct tgtagttatc tattcttacg
      661 gcggttctga actgattgct atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc
      721 ctaaagcaat caaaggtgtc atgggaagaa tcatttcctt ttacatcatt cctttgtttt
      781 tgcttttgat tctttttcct tggaatactt tagcaggcac gaatgtcagt ccttttgtga
      841 tggtatttga aaaaatgaat atcccatttg cagcggatat cgtcaatttt gtgattgtcc
      901 ttgctttgtt ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc
      961 gtttgaagga tcgaaaaggt tcctctagaa aactagctgt tttaaacaag catcaagtac
     1021 cgcaacgtgc tgttttattt tgtaccagtg ttctttatct tggtgtagct ctttcttatt
     1081 ttgtcggtga caaattattt ggctatctgg caggttccct ttcgtataca gtattattga
     1141 tctggatcat tatcagtgcg gcatcctttg tactggcact aagaaaagga acattatggg
     1201 ataaaggaat cagctttttt gcattagctg tcttaggatt gattttcctt ggtatcctgt
     1261 tcacgaactc cattggggta actgtattga ctggcctgct ttacctgttc atttatttca
     1321 gttatcaaaa aaagaatgat gcgttcgtat tgacgaatga atgaaatcga tagaaaattt
     1381 tattttattt atgtggaata gatggagcta ttgaagaaag gagttgtgac aaaagtcttg
     1441 ttgcaactcc tttttccgaa taaacagtct catgacctct tggtaaacgg tgttcaaaaa
     1501 ccctctgtgc aaaataattg attgttctcc atgctcatga catgatagaa gaaaggaatg
     1561 aaaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat gatcggtgtt
     1621 tcaacggtag ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga tcatatcttc
     1681 ttcagtcgta cacgagtatt catggcgttg atgatgggtg cagtcatggc agtcatcatg
     1741 ctgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgttatgat cttagccgct
     1801 agtcttcttg tttttgccgg ttccttgttt atggtacgaa gccaaactac tgtcaacgac
     1861 acagcttgga tgaaggccat gattcctcat cattctatcg ctatcctggc aagtaaacga
     1921 gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa
     1981 gaagagatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa
     2041 ataaaaaaag aggatgttcc agaagttatc agcttcaaga aataagaaga agctacttct
     2101 ggaacgccgt gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
     2161 aaacaaaaat agcttctcct atttttcgtt tggttaggct tattaccgca gatgcaagct
     2221 tttgaacacc gtttattaag aggttgtaaa atcagcgttc tgacctgagt attagagaga
     2281 caaatggaaa aatagttcct catattttgc cattttttga gctaataccg agggtcagct
     2341 gattgaacac cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg tattaagaaa
     2401 aattttgtga aatggctttc cacatttttg tataaatttg gcttaatacc gagaaactgg
     2461 cttttgaata ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca caatcccttt
     2521 ttatgttttt attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat
     2581 ttttttgaga ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg agaaagcaaa
     2641 ctgaaaaata cgaatggcaa gtaagtgagt gtaggaatac caaatgtact tgctacgaaa
     2701 cttccggcaa ttccccaagg cactaagtag ttgattactg ttccaccatc ttctagcgtt
     2761 ctacctaata cagttggatc aaatcctgct ttttcataag tttctttaaa tgcattacca
     2821 ggcagaataa cagataggaa ttgttcacca ataaaaatat ttactccgat acttgtcatc
     2881 aatgttgtaa aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg ttccatcacg
     2941 gttgagatca agccaaattc gatcaacaat ccacctaatg acaaagttag aataatcaat
     3001 gacacagtag gcatcatact ttcgattcct ccgcggctaa gcagtgaatc gatttgttgg
     3061 tttccagtat ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt taaggaagtt
     3121 tgtgggtttt gaataaaaat cattcctact gctacagcga tattcaacaa aatcgttaaa
     3181 attgccggca cctttttcca tgcacacagt aacattaaaa ataaaggaat cagtgaccag
     3241 attgaaatag taaagtttgc ttctagaaca tcagtcactt cacgaataga tgaaagactt
     3301 gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac aaatgccggg
     3361 acagttgacc acatcatatg cttgatgtgt ttgaacaaat cggcatcaac aatagctgct
     3421 gctagatttg ttgattcgga taatggagac atcttatctc caaaaacggc acctgaaaca
     3481 atagctccaa caaccagcgc tggatttaag cctagtgttg tcccaatacc gaaaaatgca
     3541 atcccaatcg ttgacataac agtaaaggca ctccctacag cgctacctac gattgcacac
     3601 acaacaaata cagaagggac aaaccactgg acactgatca atttgaaccc aatgaccatt
     3661 agagttggaa tgatccctgc ttgtatccat acagcaatca atgaaccgac taaaataaaa
     3721 ataaagattg ggatgattcc tggtttgatg ccattgatga ttccttcgtg cagccaatct
     3781 gtcgggtatc tttttatcac tgcatacaac atgaccattc cgatcgctgc taagatcgtg
     3841 atattaggtg agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac aataaccatt
     3901 aatacaagtg ttgcttctaa aaaacttggt gttccttttt ttcccatgat aatctctcct
     3961 tttttagttg ttcttatgtt ctaaaaaagt taagatatcg gattactccg ccgcaattca
     4021 tccgcgattc ctcctttata aaaataaaaa gacctgtttt aaactaagga cgaatgactc
     4081 cgcggtacca ccttaattaa aaacagatct gtttttcact ttcaaacttt tattcaaatg
     4141 tatggacttt ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga ccaccgactt
     4201 tctgaacaaa acacctaacc gctactgcgt gaaaagttga cttgtacgaa gtttaccttc
     4261 cataagatca tttgtcaatg aaaaagaaaa tattttttca tttttctgaa aattattttt
     4321 ataactttca cacacaagtt tattttttac tagaaagtag cgccctcttg tttttttctt
     4381 gctactattt cttgatgttc ttgcttgatt ctttctaata gctttggctc tttcaacaga
     4441 tcaagagctg tattggcgag tagttctgct gcaatcgcaa tagaagcaag gcctttgtca
     4501 cttttagcag cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc agagattgct
     4561 accgtaggct ggattgttgg tatgacttgg ctgacatttc ctacatcaga agaaccgaga
     4621 cttgttcttt gttccgtttc tatttcttca tctggaacac ctgcttcttt tgcatgctgg
     4681 aagaataagt catcaaaaga aggagtaacg atgacatcat caactgcatt ttggaacaat
     4741 ccaaactcaa aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt ttccactttt
     4801 tcatatactt tatctaatgt ttgccgattt cctgcacgta aatagaaacg tccagcagcg
     4861 taatctggaa tgacgttggc agctactcct ccgtctgtaa tgatcccatg gatattgaca
     4921 tcttttggca attgcaagcg tagagcgttg attccattaa acacttggat caatgcttcc
     4981 aaagcattta ctcctttttc aggtgctgct gctgcatggg aagcgacacc gtaaaatttg
     5041 atgtcgactg gatcattcgc taaagattct gtcgttttcc cataacgaaa agctggatgg
     5101 acgcataacg ctacatcgac atcctcaaaa aaaccttctc gaacaaagct gcctttagct
     5161 gatccgttct cgcccccttc ttcccctggg gtgccatata cacggatttc tccaccagtt
     5221 tcatcaatga attgcttcac tatgctggcg gctaatacgg agtaagtacc aaacaaatta
     5281 tgcccgcatg catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa ggcaattgtg
     5341 ggaccgggtt tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc agctacttct
     5401 ttttctaccc aaaacctttc tttttctaat tgtttaatca acgctgctga ggaataaaac
     5461 tcatagttac ttacttcagg atgttcgtgg atatctagag caagtgcttg atattctggt
     5521 aaacgatatt ggattagatc cttcaattgg tttgttgaga ttgttgttgt cataaaaaat
     5581 tcctcctatg ctagtcattt ttcttatttt ctcccgaatg tttcccatgc aggcacgctt
     5641 gatccttttg atgttttctc gattactttg actgtgtctt ctgtttgata agcgtctact
     5701 attttttgat acgttttatt gttttcatct tcttttcttg caacgatgat attcacataa
     5761 ggacgtgcat ggtcatccac tggttctaag aagatggcat cagaagcagg attaaatcca
     5821 gcatctacag ctacaccgct attgatcact gaaatgtcta catctgacaa agcacgtgct
     5881 gtttgggaag catctaattc agaaatgtct agattttttc tattctcggt aatatcagaa
     5941 acagtgggcg cttgtccttt tgccttatcc actttgatca atccagcagt ttgcaataac
     6001 agaagagctc tgccgccatt tgtaacatca ttagggattg ctacttttgc tccgtctttg
     6061 atatcagcta catccttcaa agtgttagaa taaattccca gcggtgcatt gacagtattt
     6121 ccgatcgata ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa gaattgatgt
     6181 tggaaagaat tcaaatcaat cgatccatct gctaaagcca catttggttg attgtaatct
     6241 gtaaatttca cgtattccaa attaatgttc tcctttttca aacggtcttt cacgttgtcc
     6301 catacatctg tatcttctcc aataatccct agttttacga tagtttcttt tttgtcagtt
     6361 gtatctgctt ttccttggct acaccctgcc aaccaaataa ttgctacaaa tgtaatgact
     6421 acaaaacgaa taattttttt cattttttct cctccacttt caatgtgtaa tttttttaat
     6481 cagttgatta ctgataaatt gacttaaaaa aactaagatt agaatcaata atgtagccac
     6541 aatcgttaca tctgtttgga atcgattata ccctctcgat atagcaagat tccctaatcc
     6601 gcctgcacct actgctccag ccatggtagt cagtccgatc acgttgatga tagtcaacgc
     6661 cgagacccga atgataccag gcaaaccttc taacaaatac acacgtaaaa tgataccgat
     6721 tggactggtt cccatcgctt ctgccgcttc gatcacccca ggatcaactt ctagcagtgc
     6781 gttctcgatt tgacgagcaa aaaacggaac aactccaaaa atcaatggca ctaaagctgc
     6841 tttcgcacca atcgtcgttc caactaagaa acgagtagtc aatgctaaaa gagaaagcaa
     6901 gataatgaaa gggatggaac gaatgatatt gatcagctta tcaagtattt caaatagcag
     6961 acggttagct agtaatccat tagggcgtgt cactactaac gtcacaccta agatgattcc
     7021 aatcatcccg ccaatgaatg ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc
     7081 gacaaattca tccggaatca aataaacatt gggcaaatat gttttaaaga aatcagtcat
     7141 tttatcttcc tccttcgatc actttgatac gactatctaa atcgtttttt tgtaaaatat
     7201 ttatttttac accagatttg atcaaataat ccaatgcttt tttccgctgt ttggtttctc
     7261 cagctaaagt aacgatcaaa ctccctaacg gcgtttcttg taataactct acattgccat
     7321 ataaaatatt cgctgttact tgatatttgc tgtacagatg agcgattaga ggttcgttcg
     7381 tctgatttcc aatataactt aattccacca tccatttatt ggcgatctga tcggcaaaag
     7441 cagaatgggc aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt cgaataaaat
     7501 ctttggtcaa ttcttcttca ggattgctga agatctggat gctgctacct ttttcaacga
     7561 ttcttccatc ttccataacc gctaccttgt tgcagatttc tttgacaact tgcatctcat
     7621 gagtgatcag gacgattgtt aaacctaact ggcgattcaa tttttttaac aatgcaagga
     7681 tctgaagggt cgttttcgga tcaagtgcac tggttgcttc atcacataat aataccttag
     7741 gttcattcgc caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt
     7801 taggataagc agatgctttt tcttccaaac cgacaagttc cagtagttca ttcacttttt
     7861 gacgacgttc ctgtttcgat tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa
     7921 tagttcggct ttccattaga ttgaaatgct gaaagatcat accgatggcc tttctttctt
     7981 ttcgcaattc tttgggagag agctgactca gattcgtctg attgacaatg acttctcctg
     8041 ctgttggttt ctgcagcaaa ttcatcacac gaactaacgt acttttccct gcacctgagt
     8101 aaccgacgat tccataaaca tcgccttttt caatcgttaa atctacagaa tctactgcct
     8161 gtatctcttt ttctttttgt ttgaaacata cagaaacatc tcgcaattca atcattaaaa
     8221 ttcctcctta tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg
     8281 cttttttgcg tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt
     8341 acgatcatac tctagcaaga tatcggtcgc agccgtcatc gccttacatc tatgctcggg
     8401 atgaattgct cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag
     8461 ctctctgtca aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa
     8521 aaagtccttt tactaacata aactatgtca gtaaaaggac gacgtatcgc ggtaccacct
     8581 tttttcggaa gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt
     8641 ttaacggtcg aacagttccg aatttgccta cttatcgaca aattattctc caaggccatt
     8701 ttcagctttt cttacttgac tcttctcacc agccagagcc tctctgcaaa gtttccaaac
     8761 ttactttcct tttcaacgaa atcgatattt tgttgttggg attaatctac atgaggaaaa
     8821 ctgaaaagtc aagtacgaaa ttcaaattat tttttctata tttatttttc tgttttttta
     8881 tttaacattt caataacagt tcgaagatct tttccttccg caagaagttt caatttttcc
     8941 ttttcttcga cggtataatt tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat
     9001 cctgctcgtg aagcagctgt tgctcctact aaagaatctt ccactgcaaa ggctgaacta
     9061 gcttctaatc ctgttcgttc caaagctgtc agataaggaa tcggtgaagg cttgttctcc
     9121 gacacttcgt ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa gtgatccaag
     9181 atatctgttg ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat atgacgttct
     9241 tttaagtctg ccaatatttc ttttgcatat ggttttaagt ggatggctcc gcttgtcagc
     9301 tgttgataaa aatattcttc acgcttcgct cggacttcct gtactttttc agggctacct
     9361 gctgctttca ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg
     9421 atttcttttg ggatatggat atcaaattgc tgaaatcccc aaagccagcc atcacgatac
     9481 gttttttctg tgtcaactaa caaaccatct aaatcaaata ttgcaccttt catctataca
     9541 ttaatccttt ccaaaacaat acacatagtt tagcacgact ggtccaaaaa gaacagaagc
     9601 aaagaaccag ccttctcgtt tataagagtt cttggaatcg acggatatag attttcttca
     9661 cagctgtcac aagcgccaga taagcgatga ctgtagcaaa taaccataac cagtaattgc
     9721 caggaagtgg catcaagcct aattcagcac caaaacttgt aaaaggaagg acagaaccga
     9781 caataattcc taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt tggataaatg
     9841 gtactttcgg tgtacgcagt gcatggagca ctaaggtttg tgtccatagt gattcgacaa
     9901 accatcctgc atggaataat gcgatgaaaa tcactcgctg tgaactatcc aatgtatgga
     9961 aacttccgcc cactacagca ggacaaataa caaaatacat caacaagtaa gtagtaatat
    10021 caaatatcga acttgttgga ccaaagtaaa ccatgaattc cccaatacta gatgcattcc
    10081 atttttttgg ctcgtccaaa tattctttat ccatccgatc ccatggcaag gaaatgcagg
    10141 aaacatcata gatcagattc aaaaacaaaa gttgtaaagg cagcattggt aaaaatggca
    10201 ggaacgtact tgcaatcagc acggaaaaca tattgccaaa attagaactt gctgtagcct
    10261 tgatatattt catgatattc ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa
    10321 tcgtcaaatc tttttctaaa aggatcacgt ctgcagattc tttcgcaata tccacagctg
    10381 tatctacaga aataccgata tcagccactt tcattgcagg agcgtcattg ataccatctc
    10441 ctaaaaatcc gacagtatga ccatttttac gtaaagctgt cgtcaaacgt gctttttgct
    10501 gaggattcaa ttttacgaat acttcatggt cttcagctac ttgtgctaat tcatgatcac
    10561 tcatttccga gattttctca ccggtaacca attcattgat ctctagacct acttgacggc
    10621 acactgaacg tgtaactaat tcgttgtccc cagtcagaac ttttacagct acaccatgtt
    10681 ctttcagagc tttcaacgct tgtttcgttg tttcttttgg tggatcaagg aaagcaagat
    10741 aaccaatcaa aaccatgtct gactcatctt tgactgaaaa ttctccaacg acgcttggat
    10801 tggtcttttg tgcgacacca atcactcgta agccatcttc attaagtgac tgtacttttg
    10861 caagtacggc ttcttttcgt tcttttgtta atggtgtaac agtaccgcca agatcaataa
    10921 atttagaaat gttcagcatt tcttcaatcg cgccttttgt gatcatttgt gttttaccag
    10981 ctggatcagc aaccacaaca ctcatacgac gacgttcaaa atcaaaagga atttcatcca
    11041 ctttttgata attgatctta tctgtatcta aagtttctgt tgcttcatca atgatagcca
    11101 catctaacaa atttttcagc cctgtttgat agtagctatt tagatacgca tggcgcaaaa
    11161 cacgatcatc ttcatttcct tcacaatcca tatgatattc aagaatgatt ttatcttgtg
    11221 tcaatgtccc cgttttatcc gtacataaca catcgattgc accaaagttc tggatggcat
    11281 tcaaattttt gatcactgtt ccttttttgg ccatcacact tgctcctttg accaaattcg
    11341 tcgtaacgat cattggaagc atttcaggtg ttagaccaac tgctacagat agtccaaaaa
    11401 gaaaagcttc tagccaatcc ccttttgtaa gcccattgat gacgataact gctggagcca
    11461 tcaatgacat aaatttgatc agtagcatgg atgttttatg gatgcctaat tcaaagcttg
    11521 attgaattgg tttttcagat agcgtttctg ctacatgacc aaatagtgtt tcatttccaa
    11581 cagaaacaat gattccttcc gcacttccac tgatgacatt acttcccata aagacaagat
    11641 tttcgtaact tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc ttttctactg
    11701 gatagctttc ccctgtcaat gctgcttggg aaatgaataa atcctttgcg gacaataaac
    11761 gcatatccgc tggtatcata tcccctgctg aaagccgaac aagatctcca cagacgattt
    11821 cttccatagg aatctctgtg tactttccat tacgttttac agctgccgtt actttcacaa
    11881 gtgatttcag tttttcagcc gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc
    11941 cgctgacgat aaccatagtg aagataatgg ctacaccgaa aagatcttta tctccaggag
    12001 ctgctaatac atattccgtg atgaatgaaa taattcctaa tgcgatcaac actaatgtaa
    12061 atggcgtgat gtatgctttc aatatttcta caatgaatgg tgtttttttg ccatatgaaa
    12121 tcgcattttc accgtaattt tctcttgaac gttcgatttg ttcttctgtc aatccatttg
    12181 ctgatgcatg gaatagttca aataattctt gtttcgtttt tccagcaaaa gctgcataga
    12241 ttttttgtgt atctttcttt ttttccatct caatctctcc tttgtgtcca aaagacagct
    12301 gttctcgctg cttttggtct ttagctattc atcggatcga tagagaaaaa actgaaaggg
    12361 gctgagaaca gatagataat agccctggcc tttcctctag atccttgttt ttcgaatgac
    12421 tgtcatccat ttgttctcat ctcctttact aagtaatttt aattcatgac ttccaatcat
    12481 ttttcgaata gttatttttc caattagacc acctcctttt tatttttcac cgatggggat
    12541 ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcatatta
    12601 aaaaaagctt ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata
    12661 cctgctttta gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc
    12721 gtctctcgac gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatct
    12781 tattcggttt tccttcgtta atataacaac taaaaaagag gatgtcaaat caattttcca
    12841 tgtttttcat acttttttta aaatacacac gttatattcc tgtctcacgc ggttttattt
    12901 gtctcatttt ccacctcccg ctataataga aatatagtag gaaacgaggt gctgttatgg
    12961 aaagaacact tgttattatc aagccagacg gcgtgagacg ccatttagtc ggaagcatca
    13021 tccaacgttt tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa actatgactc
    13081 ctgaattagc aaaagaacat tatcagcact tgactgagcg cagttttttt gatgaactga
    13141 tcgattacat gacttccggt cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg
    13201 atatcgtccg taagatggtt ggagcaacaa aagctgccga tgctgttcca ggtacgatcc
    13261 gaggagacta cgcgctgccg gggactgaaa acatcattca cgcttctgat tcccgtgatg
    13321 ctgcggtaaa agagatcgcc cgctttttcc cgccattcga tataaacaaa ccgtctgaaa
    13381 aagcggcgaa gtgatacaaa ttctaaactg ctggtctagt tgctgggttc agcaatggcc
    13441 agcagttttt tctctaagga cctccgttga ttctttccta acatatattc gagcactgtg
    13501 aagaattctt ggtagtcttt cgtcttttct tcccctaaag cagcagaaat caatctttga
    13561 taataatctg ccatcaatgt ttcatagatc actgtacttc tttctttatg tgataagctc
    13621 cgtttcatat cttcattcac actacgtgcc tttttcaacc gaccattttc tatataaaac
    13681 gaagtcaggt tcattaaaaa agtaaagaga tactgctcat gattgttcaa ctgtgctaat
    13741 cgtcgcattc tttttttgat tttcaaataa ttgttctcaa tgaaccgatc actgaatatc
    13801 caaggaaaat cagctagaat agatagttca aattgaccat agtctggtat ctgcatcaag
    13861 tattcttcaa tgattcggca ttcataacgt aaaaattcaa attctgtctc agatacttct
    13921 gttttttcga ccatgtgaag attcaaaacc aaataataca gatcttctgt tttcttaaaa
    13981 tgcctacggt tattgctgat ctctttataa ggatcaaagc tgtcttcttg tacccgatct
    14041 agcattcgtc tttttttctt taactttatt ggttcatcca gctggtttcg catccattca
    14101 tattctgcaa gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac aaatgacatt
    14161 tttttcactg aatttgtctc aatcccgact agagaagtct taaaacagat tccttccgct
    14221 aattgggact gtgtcaactt cttgttgact cgaagtttct tgatcaattc accatcatac
    14281 ataatagttc tcctttttaa tttaaatttt tccacattca ataaatagat acgttaaaaa
    14341 ggaaaaatac acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc aaccttcaag
    14401 cattagaatg ttgtctaact actgaaggtt ggtttgattg tttattgatt atttttttgc
    14461 tgttcaacgt ctgttcttgt tactggatat aagttggttg ttgttttatt accatttttt
    14521 gaaaagacac ttgtcgaatg ttcgcctttt tgcttttcga ttgcttcatt acgttccgct
    14581 tcatttttat agtcatattt tttcggatca atcgtctcaa atccgtttgg cacataaaac
    14641 cgcaagagat tttcctgatt cagcttatct gaaagactta acgcagtctt gactttttcc
    14701 tgatctttgg agatttcctc atcgattcct gctgctttcg tatcgatgat ttctcctgtc
    14761 tgattattgt aagcttttcc tcctaggacg gtgtatttgg gtgtcacaaa gttcccattt
    14821 cgaaaagcaa ctacttgatc atgctgagaa gataataagt ctgtaccaaa atgaagataa
    14881 tctttatcat cgattcccaa tagatgtagt aatgtcggca aaacatcgat ttctccaccg
    14941 tattcatgat tgactgtccc tttagtataa ccaggcatat ggatcatcaa tggcactcgt
    15001 tgcatctgtg cgttgtcaaa ttcatcccat gtatctggat ctttaccaag agcgcttgct
    15061 aaatctttat tatctgtatt cgagatccca aaatgatccc cgtaaatcac aaagattgaa
    15121 ttttggtaaa taccagattt tttcaaatac gtaaaaaatt gttccaatga ttgatccaga
    15181 taatgtgccg ttcgaacata attatctact gtgctattgc cagtattcaa agatggaaag
    15241 tcgaaattct tgtcatccgt ataataaggc gtgtggttgg ttacggaaag aaatttcgtg
    15301 taaaacggct gttgtagatg ctcaagatat tgggcagatt ctctgaataa atctttatct
    15361 aatatcccat aacctaacgt ttcgtcggat tcatcaaaat aagatcgatc aaagaaattg
    15421 tcataaccta agtttttata tacatgatct ctattccaaa aactagctac attcccatgg
    15481 aaaacagcgc ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca
    15541 ttgtctgacc caagttctgt gaataatgag ccttgaggta aaccaaatgt gccagtttcc
    15601 aacatatttt cagcatcact tgtctttccc tgtcctactt catgaaagaa attgtcaaag
    15661 ctgatcgttg cttgattctg gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct
    15721 accttcatat taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat cacatttttc
    15781 cctttagcga tcccaaatgt ttcgggattt ggctctgcat aatgtttctt tgtataatct
    15841 aaaacttcat caatcccatt actgcttgca tgcgcgcgaa cactgctagt cattcctgtt
    15901 ttgattccgt cataaatagt atacgcatcg attcctagat atttgacaat atatgaacgg
    15961 tcaaatgttc gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa
    16021 aaaataagcc ctgaaagaca ggtaacagcg atagccatcg gtttgccgac agggttgctt
    16081 ttgataggta ttttcttgac cttcaaccaa accagtagcc caatgaacac agcgatatcc
    16141 agccagtaga tgacatcatg gggcttcatc aaagaaaagg agcttcctga caatccttgc
    16201 gacactttcg aaaagcctaa tacagatttg actgtaatga aatctgtgaa ttcccgataa
    16261 aaaataatat tcaagtacaa caaaagcgta tttaaaatat caatgatcaa aagaatagct
    16321 aacgctggct ttggcttttt aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca
    16381 attgggttga tccacaagat aaagtactgg atcgtacctt ccacacctaa tgaaaaatct
    16441 gcatagtagg caatgatcgt cttcagccag aaaaagaaaa cagctgtcaa aacgattgct
    16501 aaacgatttt ggcacaattt tttaagttgt gtcacgtata tcacttctct ctattcacat
    16561 tttttcttat tttgctcctg aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt
    16621 tttttattct ttctaaagat tattccgtcc acttttcaca gcataaaacc gctgcgatgt
    16681 aagcgattaa actaatgaag agataaaagt cttgtggtga ccgcttcact ccacggtatt
    16741 caaaagccag tttctcggta ttcgtacaaa aaaagaggct gtgacaaaat tcttgtcgca
    16801 gctccttatt tcttgatgca ggacagcttt ttcacaactt cttccttaaa cggtgttctc
    16861 ctgccaactc ctcggccaca agtcacaata tttataaaac atgagaagct gttttttgaa
    16921 attgttcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc
    16981 aatcagctga ccctcggtat tagattatgt caggacgcta attttacaac ctcttggtaa
    17041 acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atataaggag
    17101 ctattttttg aaattgctcc ttgtggctca gagttaaacg gcaggctcaa gacctcttga
    17161 tacacggtgt tcaatcagct gaccttcggt attagctcaa aaaacggcaa aatatgagga
    17221 actattttgc cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctctgt
    17281 ttttcattgt ccataggatt gatattgtcc ttgtgatgac tcattcgtaa taattgaggc
    17341 ttgattttct tcacttcccg ttatcgtttg tccactgcca gaatctgaag gacttgttcc
    17401 tgtcgactcg tttttttgag aagagacagt tgttttcttg acttgttcat ttgtcttttt
    17461 ccattccgct aaacgatcgg cgaccacttt tttgtctact ttttcagaat aagcggtgat
    17521 cacttttcca tttttcaaac gggctacttt gtcgccatca tgagtcaaaa gcaaaacggt
    17581 caaatgcttg tctttaaatt gatattgtct gagagaaatc gcactaagta cgtcatcgat
    17641 attaataaat tgattttctt cttttccttt tggcaaataa atgtccaatt cgcctttttc
    17701 gtaattggca gttattttat actccgccca ccaataagtt gagaattctt ttttgaaatt
    17761 atctactgat tctttgcttg ctacgatttt ttctgtttct ttctgagcaa gatctgctgt
    17821 catttttcct gaaaatgctg cggataaaat aagcagtaag aaaaaaccga ataaccaaaa
    17881 gagaagaaac ggtctttttt tcctcatgct ttcctccact cctatttaat tttgactatt
    17941 ctattatata caaaaaatga aatagaaaga caaaaaagtt gaaaaagatc gtaaataatt
    18001 gaacttcttt ttcacaaaat ggtaagaatg gagaatttca cgcatttgct ctcaactatt
    18061 ctttcttaag cctcaaatcc gtttttatct gatacgtctt tgaaccaatg acccgatttt
    18121 ttgattgttc tttttccatc ttgtgctaag tctactgaaa taaagccata tcgattctta
    18181 tatgcattgg tccacgacca gttatccata catgtccaca tatgatagcc taaacaattg
    18241 gcaccttctt gaatggcttg atggacatat ttcagatgag attggacaaa ttcgatccga
    18301 taatcatcgt ggatcatgcc atcttctttg ataaatcgtg cttcgttttc tacacccata
    18361 ccgttttcag aaataaagca gtcgatattt ccataatgat ctcgtacatt gatcaaaata
    18421 tcatagattc cttgttcata gatctcccat ccgcgataag gattcatttt acgaccaggc
    18481 atttcataat tgtcgaaata atcctcaggc attggaccgt ttttagtatc gattggtgtt
    18541 tcttttgctt ttacccggcg tggctggtaa taattgatac ctaaaagatc tactgtattt
    18601 tctttgataa ccgtcaaatc ctctgcatgg atcacaggca tgagatcaag ttcttttact
    18661 atctcaatca attcacttgg aaactcgcct tttacagctg gatcaaggaa tgaacgattg
    18721 aagaacgcat ctgctatttg cgctgcttta acatcttcag ggtttgattc gtctcttgga
    18781 tagcttggtg tcagattcaa gatgatacca ataacaccac cttgctccat ttcatggtaa
    18841 gatttgatgg ctaacgctga agccaacgct tcatgatagc caacttggat cgcttttttc
    18901 atatctgact cttctggata atgccatcga tataaataac ctgcttcgac tggaacgatt
    18961 ggttcattat gagtaaacca tttttttact ttcgaaccaa acaattcaaa acaacttttg
    19021 gcatattttt cataggcttc aaccgtttct cggttcaacc atccgccttt ttcttgcaga
    19081 tacatcggca tatcgaaatg atacagattc acaaagggtt cgataccgtt ttcaatcaac
    19141 tcatcaaaat aacgatgata gaaacgaacg gcttcttcgt tgattgttcc agattcatca
    19201 ggtatcaaac ggctccattg gatagatgtt cgaaatgaat tatgcccagt ctgtttcatc
    19261 aactgtacat cctcttgata cttcgtataa acttgcgagg ttttttcagg tcctacttga
    19321 tcaaagaact tttgcggttc ttttttgtac caataatcaa aaatgttctc tccttttcca
    19381 tcgcctgaaa tccttccttc tgtttgtggg ccacttgctg cagaacccca ccaaaaattt
    19441 gctggaaatt gatatttttt catcaaagca cctctttttc ttttttagtt cgttttagtt
    19501 gattccatgt atgatatttt ccatcacaat cactttttca ttttcttcct cttctttttt
    19561 gattcgtttt aacagctgtt ctgctgctcg ttccccgatt ttatctgttg gtggcgtgat
    19621 cgtgattttc ggaaggatac tcagatcgtt tttatctaaa taatcatagt gactgaacgc
    19681 aatatccttc ccgattctga caccttcttg attgaaacat tcgatggcac caatcgacat
    19741 attgtaattg ctgacaaaaa tagcagttgc ccctttttct aataatgctt ttgccccgta
    19801 atagccgctt tttctagaat agtctcctgt aaagacctct aattggatcg atggatcatt
    19861 ttggaccgct tctttggctc ctgctaaacg ttctcttgct acgtagtctg tttgagtggc
    19921 agcaataaat ccgatttttt tatgtccttg ttcaccatga tgagcgatca cttttgctac
    19981 actttctcga tcattcacta atattgtatc aatacctgct ggtccttctg gacaattcaa
    20041 tgcgataact ggaatactca tttttgtgag catttcaaat ccagaccact cttcttcgga
    20101 taagaagaca atcagcccat cgatcacatg cttcatcagg tattcaattt tttgttcaat
    20161 tttttctggc tgatcggcaa acccgcttaa taataagcta tacccatttt cttcagctac
    20221 tctttcgatc cctgagacaa cttccgaccc aaaacggctg gaaatcccgt ttatcaaaag
    20281 tccaatagag aagcttcgat tattcttcaa ccctttagca atgatgtttt ctttatagtc
    20341 caacgctgaa atcgcttctg ctattttttt catgttcttt tccttcagtt gctgaccatt
    20401 caagtaacgg gaaactgttc ccactgaaac accagccatc ttagccacat cacggatcga
    20461 tgccatacat tctcactcct gccagttaca tttgatttct ctttcttgtt gttcgattat
    20521 gatgaccgca ttctttgcat agtgttgctt gtcatcaata tccatataac taaagtcttc
    20581 agtagagcaa cttcctctca ctattaatta tacttaaaca agtatttgaa ccggttcatt
    20641 tttaatatag cattttttta aaagattgca acactttttt aaaacgcttt ctgaaagtgg
    20701 cggatttttt gcctttttaa aattttattc taaaatttac tctatttgtg ggtgttttgt
    20761 gcgtttttct cttccatccg tttgaagttt tacattccaa tggtataatt tctttatcct
    20821 atcaaatggg ggaagtattt tgaagaaatt aatcgtacta tttattttgt ctgcgggact
    20881 tttgacattg actggctgtg atttgtttgg cggctctgac caatcctcgg acgaaacaac
    20941 cgattcttct gtcttaaaag cggaaaaagc agataccacc aacagtgtat atacgctaaa
    21001 agtacgtaaa gatatggatt tatccgcggg acagttgaca tttacagata aaaaaatgga
    21061 atggacacgc acgtatcaga cacaaggaac tgattcttct gaatcatctg aagaaaaaac
    21121 agtgctgaca gatatcaaga tcactaccaa agatgacgaa tatattattt caggaaaaga
    21181 aaataaaaaa gaaacaacag tcatatttac aaaaatcggt aattaccgtt tgaaggatga
    21241 ggatggcaac atctacagtc tatagtgccg tctgttggat cttcttttgt ttctcccctt
    21301 cttttctgct atagtaaaga agcaagatga ttgtaaagga gacgaaatgg atgatcgata
    21361 gtcctgatta ttgtacatgt aataacaaca aaaatatcca tacagaattt aatgaccaag
    21421 gaagcttgag catatgcgac aactgtcaca aactagtcaa agattcattt aaaacatttg
    21481 aatcttatcc tgttcaaaat tatgtaaaaa gtagaagaag ataaacaaat ccaagcaaaa
    21541 ctcactatgg ctgattttta tgaacttcaa actcagcttc ttcctttgat caagcttcat
    21601 cagtaaaaca aacagtggat catttcagtt tacaaatcaa agagtaaaga tttatttatt
    21661 tcaaatattt gaatataaaa taaccaaacg atagaaaggc ttattctaca tcacgtagta
    21721 atttttcttt cccgtcgttt ggttttttat ttttagccta attcttgacc ttttgcatgc
    21781 tggttatccg taaaatattt tccagcaggc tttccttcca attcagctaa tttttcttgg
    21841 atctgacgga tcaccttttt tgcatcttct ggattttcta caaaatcgaa tttatcccca
    21901 tccacttgta ttttaggaca tacatcaaaa tcttcatacc attgattata gcgggtgttc
    21961 aacgtttcat aataggtata taacgtctca tcgctttcta gctgttcata gtcacgccca
    22021 cgttttttga tacgttctag catcgtgtcc aaagaaactt tgatatggac catcaaatca
    22081 ggccgttttt ttggagcaac atcatctaac tcgtttagca tcgtatctaa taagctatcg
    22141 tactgctgta cttcgatatc cgttacccga cctaaatcag cgtttagatg gaacaacagg
    22201 ctatcttcat agatggatcg gtctagcaca ttgtcatcgt tcactaaagc atttttcatt
    22261 gctaaaaatc gcttgttcaa aaagaagatt tgcaataaaa aagcgtactg ctcaggattt
    22321 gaataaaaga gcggcaatac ttcgttatct tcgacatttt cataaaatgg tttagaatca
    22381 atctcttttg caagcatatc cgtcaaactg ctcttacctg caccaatcgt tcctgaaatc
    22441 aaaatcactg acatacaacc cttcctcttc tataagtact cgcctacgta agtttcaaca
    22501 ggcaatgttt caaaatcata ttcacgtaca gcttggtctg attttaaata cgtatggaga
    22561 ctttgtgtaa tcatcaagtt cgtaggtacc gtctcttctt gtacaatgat catcggtttt
    22621 ttcaacatag ttgccacacc taattcgtaa gccgttcctg gatcgattgt ttgatgctca
    22681 aagtcaagga tagcaacaac aaattctgca tttgtcagtt cttccatatc tttttcgtat
    22741 acttcctgcg cccatccagc gctgaatagt tcataattgc tttcttggtg atgacgtgga
    22801 gagtagaaac ttgtgactgt tttgttttcc tccaacgctt tttcgatacg acttactcga
    22861 tcgatttgct cctctgaaaa aaatggtcct gctaaatata tttgactcat tattttgcct
    22921 ccttcaataa taactgattt ttaatagata ctgctcgtaa ttcattgacc agttccaatg
    22981 gattgttgga aaagattact tgatccaacc attttttatc cataaaatcc tcttttgcaa
    23041 aagtttctaa taattgttgc aagccgctgt aataaccgcc aatattcaat attccgattg
    23101 gcttgtcggg cgtcagaccg attgctatcc aagaaagaag ttgtgagaat tcctccaatg
    23161 tacctagacc tccagggaaa acgaggacag catctccctt ttcaaataat aatacctttc
    23221 tttcatcgat cgtttctgta ggaataaatg tagtaacatc ttttccaggc aattcagttt
    23281 caaaaagccc attcggatag atacctgtga cttttccgtt attttccagt actgttttac
    23341 taatcactcc cataatcccg gagtatgaac caccgtaaac cagttcgatt tcttcttgtg
    23401 ccatgtattt tcctaacgtt tgcgcaataa atttgtaact ttctttatta ccaaaacggg
    23461 aaccgcaaaa aacagtaagt ttcataaaaa aacactccct aactcaaaag tctatcttca
    23521 ttctacacgt gtttttttaa aataaaagac tagattttgt agttttttag tggtatatag
    23581 gatagacgcc actatatata gtatttagaa aaaaatcatt tttcagccat tttatagata
    23641 aaacaaaaat aaataacgtg ttttcatcga tatgaaatga cttacttata gaagatactg
    23701 ggatttcgtc ctatatgcca ataacgtgta ttattttaaa caacaaatac ttgtttcttt
    23761 tattaacgaa aaaatcattt tgttctcgtg ttatgtttca ctgataaatg atgtttctct
    23821 aaataaaaaa gccgacgaac taaacgatca aaatttgatc atttttgttc atcagcttct
    23881 cctaaaaatc tttttgttaa cgataaatag ctggatttgt aaaaatatca cttgaatcgt
    23941 cactgctgga agaaaattca gtgataaccg cacctgttgc gcctgcctgg aactagtgtt
    24001 tgatattggg ctcgaacgtg tactgatctc tagcatgaag aataatttct ttttttactg
    24061 tataataact ttctttcctt tttggaatag tacaggaaag tttttttgtt tcctttcctt
    24121 cgacataagg tataccagac cctttcgaca acgaaaggtt ctcatttttc cttcatcgtc
    24181 atttcaagga gggtgccgat gctctggaca gtttcgatcg ctcctaaacc aaaatctgta
    24241 tattcgattc tttctaactc tgattctttc ataccattcc tgtctcaagg tacatctatt
    24301 gtactttttt cttgtaatcc attctgcctc tcctaactaa ttgtcaaatc atcccaagat
    24361 atgtcttctt cctctttttc ttcttggatg atttctgcat tcggatctac tggttttttc
    24421 aataataaaa tggttattcc tgtgacaaaa gaaccagcta acaatgcacc aatgaatgcc
    24481 catggtctgg tcatcgtagc gactgcaaac attcccgatg ccggaatacg gctatctgcg
    24541 ccccaaaaca tgcttaatcc gccacctact gtcccaccga ctcccgttgc tatgacattg
    24601 cgtgccaagt cgttcaatac gatagggaaa catccctctg tgatctgaca gatccccata
    24661 ggaaaagcgg tcttcaatgt atcgatttct tgacgattga gtatattttt acgaatgatt
    24721 ttccctaaga aataagcgat cgtcaagccg aatggggtta ccatcgaagc taaaatcaat
    24781 acagtgattg gttcatagat tccttctgaa aatcgtgcca caccccctta tccagaataa
    24841 atggtaatcc agaaacagcc gtgtatctgc ggtaataagc ctaaccaaac gaaaaatatg
    24901 agaagctatt tttgtttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
    24961 ctttttttat ttatgtttca taatcaatat ggtaaggatg cagctccgta tggtagaccg
    25021 cctccgaata ttcaatcatt tccccaattt gcgtagacga tttccgtatc cgtttgatgc
    25081 cgatctgtgc gtctgttttc agaattcctt gttcttcttc tgttaaaaat actgccgaga
    25141 aatcatcaga aaatgagtca atagtatatc cttttttcgt cagcaaacga tacaaagatt
    25201 ctttgccaaa ttcttctctg ctgaattgca tcatttcttg tggaagataa tgaacaaaat
    25261 aaatataagg ctgattgttc aaataatata agcgcttaaa acaaacagct tgctcaccga
    25321 aaaaggaaaa caaaggatct tccttcttta tatggaccaa ttgcaaatcc agcgtttctt
    25381 tccggacttc atatcgggag ttctccaaga tttgcgtaaa gcttgccgct ttggataatc
    25441 gattgtaagg acgattgctt agaaccgtcg tcccacgacc acttttcttt gcgacatatt
    25501 catctgatgc cagcagttcg attgcctttc ggacagtgat tttgctgact tgaaacagtt
    25561 cttctaattc actttctgtc ggcaacatcg aaccgactgg atattttcct gagaaaatat
    25621 cttctttaat tttatctgcg acttccaagt atagtacacg tgtccttttc ataacgtcac
    25681 cctttgcttt actattacta aaagaatagc acataaaaaa taaaaaaaga acacaaatca
    25741 catgtgagaa gtgtgttctt tttcattcag ctgctttaat tacaagttta cgccatttga
    25801 ggcaattact tctttatacc aatcaaaaga ttttttcttt gatcgattca gtgtcccatt
    25861 tccttcatcg tctagatcca catagataaa gccgtaacgt ttactcattt ctcctgttga
    25921 tgcactgaca agatcgatac atccccaagg cgtatagccc atcaaatcta caccgtctgc
    25981 tactgcatta gccatcgctt cgatatgacg acgtaaataa tcgatccgat aatcatcaat
    26041 aatcgtgcca tcctcttcca tatgatcaat ggctccaaga ccgttttcaa caatgaataa
    26101 tggtttctgg taacgattgt ataattcatt caatgcgatt ctcagccctt ctggatcgat
    26161 ctgccagccc cattcacttg cctcaagaaa cggatttttt actcctccca gcaagttccc
    26221 ttctgaagtt gctgcttgtg gatcagtctc attgattgcc gtagacatat aataactaaa
    26281 tccgatgtag tccactggat attcttttag caactggaag tctttttccg tttgctctaa
    26341 tttttccgga tcgactccat attttttatg ggtacgcgct gtgtatgcag ggtattctcc
    26401 acgtacttgg acatccgtgc agaaaaaatt gaattcttgg ttttggatca ttgttgctgc
    26461 ctgattgact ggattggcat cgatgctgta agttgttgca tagataatca tgcagccgat
    26521 ttggatatcc gaacgcaatt cgtggccgat ttttacggct ttgctacttg caacgaattg
    26581 attatgccat gcttggaaaa tattttggta ctctcctgct ccattgcttt ttactaatcc
    26641 ttgacttaaa gctggaaagt gaaaggccga attgatttca ttgaaagtca tccaatattt
    26701 gactttactg ctgtatctct ctaatactgt ctgagcaaat cgttcataga agtcgatcag
    26761 ttttcgattt ttccagccac cgtattcttt tgccaaatga agaggcattt cgtaatgaga
    26821 aatcgtgatc acaggttcaa tatcatattt taagcactca tcaatcagct gatcataaaa
    26881 ttctaatccc gcttcgttag gagtaccttc gtctccattt ggaaaaattc gtgtccaggc
    26941 aatcgaaaaa cgataacatt tgaatcccat ttccgcaaac aaggcaagat cttctttgaa
    27001 acgatcgtaa tgatcgattc ctcgatgatt cgggtaaata tatttttctt gatcaatcgt
    27061 ccagtcaaat tcttcactgc cgataatcgc aaagcgctgc ttacctccag gcatagcatc
    27121 tgcaacggat agtccttttc cgtctcgatc ataagcccct tctaattgat ttggcagcag
    27181 ttgctccacc ccataaaaat ttttctgaaa aacgtgattg catcctgatt tcctccttat
    27241 tttacaattg ccagaacatc ttcgtttgca ataatctctt tttggtctaa ttcaagtata
    27301 tcggcaaatg catcagtatt cgtaatgaca actggagtga ttacgctata gccagctgct
    27361 ctaattgctt cgatgtcaaa acgaatcaat aaatctcctg ctttgacgag atccccttgt
    27421 ttgactaaga tctcaaagcc attcccattt aattctactg tatccatccc gatatgaatc
    27481 aaaatctccg ctccgtcagt cgttgtgatc cctacagcat gtccagttgg aaaaatagtc
    27541 gtgatctctc cgtctgcagg tgcaactagt tctcctactt ctggatcaat agcgatacct
    27601 tttcccatca cgccagaaga aaaaacttgg tctggtactt cactcaatgg cagtacttta
    27661 cccgttaaag gactttttaa ggtatgccgt gctgaagtaa tcggttctcc tgcattcgca
    27721 tgtttgtttt ctaattgatt gtcttgtgtt tgttcgtcaa atcctaaaat caatgtacct
    27781 acgaatgcaa gaacaaaagc aattcctgta gctattactg ctactgtcac atttgactct
    27841 acaccatcca ccgtattaat gaaagtcgga atcgtcaata agctgaccaa actgctggag
    27901 aaagctttta ctccggaaaa tccgataata gctccaccaa tacctccaga aatacaagcg
    27961 gcaatgaatg gtttcttcaa aggtaatgtc acaccataca cagttggttc agtgattcca
    28021 aataacgaag tcactgtaga agaaatccct aatgctcgaa gtttcgtatc ttttgttcgc
    28081 aatgctacag ctaaagcagc cccaccttgt gccaagatgg ctggaagaag cattggcatc
    28141 aatacatcaa acccatattg ttcgatattc aaaaacatga tcggtacgaa tccccaatgc
    28201 atgccgaaca tcacgaatac ttgccataaa cttcccatga ttagacctgc tacgattgga
    28261 ctaaatccat aaatcgagtt gaagagaccg cctaaagcat taccggcaac cgtcccgatt
    28321 ggtcctaaag caagaaatgt taatggaacc atcactacta aaacgacaag cggaactaaa
    28381 atcatttgca gaaattgcgg aatcactttt tttacgaatg gctctaattt actttgaacc
    28441 catacggcta gaatgattgg tatgacagaa gaagtataac cagatggtga aagaatcact
    28501 gggatcccaa agaaactgat agcttctccc gcaccagcaa ctgctgtgat agctggatac
    28561 acaagtgcca tagcaattgc tactgctaaa aactggttgg ctttgaactt tttagctgct
    28621 gtaaaagcaa gcatgacagg taagaaggta aagacaccat ctgctgcagc aaacaatatt
    28681 tgataagtac cgctatcagc tgtcagccag cccatcaccg ttgccaatga taagaatcct
    28741 ttcaataccc cggcggctgc catcgcccct agaaaaggtg taaatatact agagataata
    28801 tcgatcagac gattcaaaat atttttggac tcttcttttc catcctccga aactgcaaga
    28861 ccttcctcat ccacgatcga ttgataaaca tctgctacat tactgccaat cactacttga
    28921 tactgaccgc cactttgtac gacttggatc acgtccgggt cttcttttaa cttttgcgta
    28981 tctgctaaag attcatcttt taatctgaat cgcaaccgcg tagcacaatg tactaaactt
    29041 ttgacattct tttgtcctcc taccgcttcc catacacgac gaccaactgc ttgattatcc
    29101 attgtttttt ccttctttct tttttattta aaaaataaaa acccagattg aaaaaaacag
    29161 tcacaaacat tcgtgaacct attttttcaa tccaggtttt gcctgccata cagtaacaat
    29221 ccttatgatt ggatgctttt tttctcactt actactcttt gaatatgaat cgttaaataa
    29281 atctgctctg cgatccccat ttcctgtttt tgcgtttgtc gaagatactc attgatccgt
    29341 tcgacacaat gaaacgcttt tggatacttt ccttgaacta atgcgtagag aaattcatct
    29401 tgctcgtcag atccttcccc tttgagataa cgctgggcaa aaaactgcag atgagtaatg
    29461 atacgctgat aattcaaaga ttcttcatcg aacacgatgc cgaagaattt actgatgata
    29521 ctcagtatat ctcttaccat tgtggtagcg gtcatcgtca cttgcatgtc ttcgtttcct
    29581 tgttggctat tcacaaaatg aaaggcaata aaaccggctt catcctcaga aagttccaca
    29641 cccgtctttt gtttcacgaa caaaagagcc tctttcgctg cttgatattc tttcggataa
    29701 aatttttttg tttcaaataa taatggatta ggcagttgga tattttcttt tgctcgtgtg
    29761 atcgcataat gaagatgatc ggtcaatgtc agataaatat taggcgaaaa ttcaactttt
    29821 agtttatctt cagccagttc gactacttgt ttgacccatt cgatctcctc tgccggaata
    29881 tcagccatca gctgctggaa ttggttttta gcaaaagaat ctcttaaaca aattcttttt
    29941 ggaccgcttc tggcgaaatc tcgtccccaa tctttttttg aaaagctagt ccttttccca
    30001 tacaaacgac ttcttccccg aatccatttt tcgaaatgac gacattgtta ttcaaaattt
    30061 tatcaatttt cattttgctt cgccctctca agaaaaataa aaaaacctag aattcgccta
    30121 ctgtttctta gctgaattct ggttttgcct gccgtacagt aacaatccat ttcttattca
    30181 aaatcatcat accatcgaat ttttatttat gcaagccttt tcatataaaa aataaaaaaa
    30241 ctggaacaca agtctatcta gaaaacaacc aaatcagaga tgcacctctt tgcgtttctc
    30301 tttttttcag caaattggtt tttccttatc gattcatatc ctttggctgt ttttaatctg
    30361 taacgactta tgtcccagta tcctaaaatg tttttgtctc tagattgaaa gcagatctca
    30421 tatcatctgt cgcatctttt atcgatgtaa cactttccag ttctccttgt acaactagtc
    30481 gcgtgacggc gtccatgtcg ccacaagtaa ttagtgtgac aatttctttt ccctcgactt
    30541 cattcagtaa ttgggtatct gtaggagcaa ctttttcttt aaaaaatgtt ttgtatgtat
    30601 acacattttc taaatctgtc aaatagatct tggcacccat ctctaaattt tctagcggtg
    30661 taaacagcaa ttcagggttc gttgcacgat ggcttgctaa ggcataattt ccttttccca
    30721 tttcttgatc tggagataat gttcctgctc cgtaaagtag tgacgtatta tccaagcctt
    30781 tgaaaaccgg taaattgatt gaaactgaag ggactgcgat actagcaatc actggtagat
    30841 ctgttcctct catttgcgac ctcatgaccc cttctgtcgt cattggttct accgcatcaa
    30901 agtcaaaact gacatcttta tcgttattct ttttcagatc ttcttttgtt acattcgcta
    30961 tagcatattt atctcctgtc tctctgacaa aataatcctt gatctgttca ttgaaaatca
    31021 aagctagtcc tactaataac aagaggaaca agaggatatt gactagccat ctttttgctt
    31081 ttcctttttt tttcattttc tctccctcca gactcatttt actgtttttc ttttgctctt
    31141 tccaacagcc gaatgacaca tttctacaac tctgtgtatt tttttcgtac acctgcgctt
    31201 ttttctacag gatatttcaa attatttttg atcaattttt cttcaatgat ctcatctaga
    31261 tccaggtgaa ggttgtctgc catcatgtat gaatagatca atacatctgc cagttcttct
    31321 ttgattcgtt ctaattgtgt ttcttttact tcttccggcg ttttccattg aaaaagttct
    31381 aacaattcag atgcttccaa agaaatagag atagccaaat ctttttcgtt atgaaattgt
    31441 cgccaattac gttcatctct aaactggttg actttttcca tactattcat ttgcttgcct
    31501 tcttccaatc acttcttact tgattaatca taccattttt ttacataaaa aatagatgat
    31561 gcttttaaaa aaactcctgt tttcttttat ttaaaataaa tgtcatagaa aatcaataat
    31621 ttaggtagcc accatgtgta tgcagtagcg tgtcttcttc ccttgaggct ttttgttttt
    31681 cgatacgttc ttcttcccaa acagacaacg ctactagaat ctctcttaat gattttcctt
    31741 cttcggtgag cgagtactct actttaggcg ggacttggtt ataggctttt cgatggatac
    31801 tttcatcttg ttctaactct cttaactgct gggtcagcat tttttttgtg atcgtaggga
    31861 tttgcttgct tctccacgcc taaaataagc gatattgctc ttccctttat ctggtgtaag
    31921 caaagcagtt agcattttga cgatcgtggg ttccgctttt cacttcttat ctacttagta
    31981 tcatgaagta tactttcgta ctaatactaa gtagcggaag tgtcaactac tttgtttaac
    32041 tacatagcaa aagaaacaga agggtgacaa cggtcatcac gcttctgttt cttttgaata
    32101 tgctgtgttg ttgtctctat ctccgtgtag actttcctca aacttatcct ttgacagtaa
    32161 gtcagtaggc tgatttgctc tttctagatg ccagatacat ttctacaaaa aaatagtgat
    32221 tctgttttga aaattcgatt tttagttctg cattttcgta aacatccaac atttttttcg
    32281 cactgtatag acctaacccg ccattttttt tagaggtttt tcctcttttg accatctccg
    32341 atagcgtcaa attcgttgga tttgccttgt tcgctatact aaaacgtgat ccctcttttt
    32401 cttccagcag attgatcgta atcggactct gatcggctac acgatgatcg aatgcgttag
    32461 aaataaaaat agataaacag cgaataaaaa caaccagatc aatcgggatt gttttcactt
    32521 cgacaggaat aactacttgg atcgggattt tctccgattc tgctttttct agaaatttta
    32581 aaaagacacc cctgacagct ggggatacaa tatttgttag ctgttgtatt tgcggttttt
    32641 caaaaatagc tgcagaatga tcttctaata tttctaaaaa ttccaacgtt tcttttttat
    32701 tttcctgaag agtaattttt aatgaaaaca gtagactttt ataatcatgt ttgaattcct
    32761 ctagatgatg gtaatattct tcatttattt gatacagttg aagcgattcc tgatgttgtc
    32821 tttcttttac ttggaaactg atcaacataa tggaaaaaaa caaagaaaaa aaactgatga
    32881 ctatcgacaa aagagataga agcagataat tagatgagcg gaagtgaaca cttcccaata
    32941 ccaagtaaat cacgccccag cttaataaaa tacaaatacc tccaaaatag tgttcagttg
    33001 gataagtaaa aagcaagttg agaaatctcg tctgtttgtc tatacttttt actaatagat
    33061 agaaaataaa tgcgataagt agtgcaaaaa aacttgctgt tccaagatgt ctctttccta
    33121 aataatccat cagatcaaaa accagaaaaa agctgagtcc agtgatagca aaaaaaagat
    33181 tactgatcaa acagctgata aacaggtttt tcgtagtaaa ataaaaaaga aacatcaata
    33241 ataagccaac taggtaagga aagctcactt tagggatagg aagtaaaagt gtcaccacca
    33301 tcaaagctat acaaccaaag ctgtttttta ctagcagttg tttcgaaaag aataaataga
    33361 tacctaaatc taaaatcaaa gtaaaggtca aataaagtgt cactgttgcg accatcttat
    33421 tcctccattg ttttgtttca aaatgtacac atattttttt aataggagcc tgcatatgaa
    33481 aaagtatttt atcatagaag ataaccagtt tcacagcgaa tggatcatca gagaattggt
    33541 caatcatcat atcgagaata tcgtgcagct cacttctatc tctcaaatca atcagttgat
    33601 ccagttgggc actattgata atcacgatgt tttctttatc gatatcgact tgaagcaatc
    33661 actcacaggt attgatgtcg ccgaacaaat acgtaaaaaa aatgcacata gtaatattgt
    33721 ttttttcacc gcttatacga attttgcagt agagatcatc aatcggaaaa tccttccgat
    33781 tggatatatc agaaaagaca ggaatttatc tgaacaaatt cgtcaagtgc tagaacagat
    33841 caattggaaa gaacagcaga tcgtaaatgt cgataaaatc tttattgatc aaaaaggtgg
    33901 aagtctcgtc ctgtatcctc gggaaatttt atatattgaa agtatcaaag gtttgaaaaa
    33961 ccaagttatg gtcaaaacaa ctacagaaga taaattagtg aatacttctt taaaacaatt
    34021 gaagacagag ttagaaaagt atgcttactt tttactttta aaatcttact gtatcaactt
    34081 atcgcaattg cagaaaattg attctgctca ttatgtgtta gaatttttca acggtgactc
    34141 cttactcgtt ggtcgaaaga tctttgaaaa gacaaaggaa cgtttccatg acttccaaaa
    34201 aactgcttcg agttgatgaa taagagctga gacgtaagca tagcagattt cagggagtgg
    34261 gccttgaatc ggctccactc cctgaaatcg ttgatatgat tttacgcttc ctcttctgta
    34321 ggagcatctg ttaacgacca tgtgaatgtt gaaacgtagt tgtctacagc ataattggat
    34381 acaggaacaa ataattccac tgattgtctc ccttgttcca cattctctcc taatgaataa
    34441 atccatgtgc caatcccttc atttgctcct gcagttacta gatctactgc ctgatctgct
    34501 gggatttcga ttccttcagc gccagttcca gaaacggtag atggcattcc actgctatta
    34561 gttgcgctat ttcgaatcac ggcgtttttg aatttcacct ttgaaccagt tagtttattc
    34621 gtatcagatg ttgtaccaat aaattctgtt cgtttgactg ataatttcca accggcataa
    34681 tttccgcttt tatccgttac ttgtacgaaa ttctgtactt gtgtttgtgc tccagtactg
    34741 tccgttccgc tttgtaatgc agcatatact gtttttgctg tacctaactt gattgttcca
    34801 aaatcaagat ctggagcttg gtcaatcgtc agtaatccgg atgttccctg atttggttcg
    34861 tcaggtgtaa aaggagtatc aggatcattt ggatcaacag gaggaactgg aacaacgtct
    34921 tcaggatttg cagcatctgg tttggtcaca gaaatcgttc cttctgtagt ggctgagctc
    34981 ttattctcag caaaaacact ttgtgtacat aataatagac ttccagctag aacggtactt
    35041 aatgcaattg tttttttcat ttctttttcc ttctttcttt taaaataaat aatccagcaa
    35101 ataagccaat aagaatgact ccaacaagta tcagccatcg ccagtcaaat gtttctggct
    35161 gattttttaa atcaatatcc ttttcattca attggtccgc tgcatcatca gttatttcaa
    35221 aatcggtatg gagattccag cgtttttctt tccaagcacc cttcacaacc gcttgataat
    35281 gtcccggagc taattttttt ccttctaatg ggacaagtaa atcaaaattt gtattgggtg
    35341 ctacatctat tttttctttt tttattgaaa ggtaaggctc ttttgtttct tttttgtaga
    35401 tttcaaccga tgtagtcaag ccgaacatat aagaagcatt tggattttga agattcgcct
    35461 taatcacatt tcttccatcg ctttgatccg cataagcttt ttttaatttc aaatctgggg
    35521 aagactcttt ttcgctattt caaatcaaga ttgctttcac ttgagaaaac tgattgacga
    35581 caccaactgt acttttgtta gaaacgttct cttttttttc ttgaaacgtg atccctcctg
    35641 cgaccacacc agccagctct ttttctggcg ctttcagtga aagtgtctca gtgaccgcac
    35701 tttgaggagg aacagttatt tctcccactg tttttgctaa gtttttcata tcataactta
    35761 atgtttcatc aaatttactt tttgattctc catattcgat cacaccgttt gaattcgtcg
    35821 tagcggagtg gatcaatgtc tcaactgtta ctgatttatc tgttcggtta aagaccgtgt
    35881 aggggatttc ctgtgttttc ccaggctcca tccttacatt tatatatgtt ttgttcttat
    35941 caatctgatt agccggtaat tcaaaatcta ccgcaaaatt gaattcggag gcatggacag
    36001 gtgtctgcca aaggatgaat ccaaggagaa ggatcataag taaagagcag ttgtttcgat
    36061 gacttttatt ccatattcga ttcatcaatc atcctccttg atctttattc tgtcggcgca
    36121 tctgctaatg tccatgttat ctctgctgag taattaccag ctatctgatt ttctttggtt
    36181 aagacaagtt taaacgcccg accgttattt aaatattctg aataatctgc ttgcacattc
    36241 gttgaatttt gtcctctgac aatttccagt tcttcttcat tcactaattg atactcctct
    36301 cctttcttga aatataggac atcctgcata gaaaatcctg catcatttgt gaaaggctga
    36361 gataatttta tcgttaagat ccacggattt ttcgtttctc gctgatcata aatccataat
    36421 ttttcctgta tatccatttg gccaaatgct tcttgtgtca aaccagttgc tttgatctga
    36481 ccaaaatcga gtttggaagg ggcaagaaat gagagaccac cttctactac gatcttcgta
    36541 gcaggtgcaa aaggcggggc atcatgataa acaactggcg tatcacttgg attgtgattc
    36601 ccaatttcat cgttggtact tggcttgttc tgcacaccct tagcagtagc ttctcctgct
    36661 tggtcgttta atacaatttc taatacatct cctgcaaaaa gttgattttc tgggatagaa
    36721 agaacaaatt cccctgtatc atcaatagtt gtttctgaca aaacagcttg attttttttc
    36781 aatgaaacca aggctcctgc ttcacctgta cctgtgatcg tgcgagaagc tgcattgatc
    36841 aatgtggtac ttaaattagc aggttcagga ggaatcacat cgactgtcgt tttggcagag
    36901 tccagttctg tcacttcttg gccacctctt acttttttag cagcaacaac ttgcacttga
    36961 tcctccgttt ttaatctctc atcattgggt acttgaactt cgaacaagcc gccttgttcg
    37021 ccttctcccc atgggttata tcctgcccct tcattttcat ctgtttgtcc ttgcgtgaga
    37081 gcagtaattt ctacagatga tttcgtcgta tctgcgtaag taatacgtaa agtcacttct
    37141 acttcgcctg tccaagcatc tcttggcttt gcctcgacac cttcgggaac aacgacatgc
    37201 ccatagattt ttttatctgc attcgttggc actcttaagg attcaatcat cgggagctga
    37261 ttattggcac tgattcgatt ataatttttc attccggcac ctgtctgagc aaattcattt
    37321 gttaaacctg cactagggct tgatacgaaa ttgcctaagt cggtcccatt gatcgataag
    37381 tctgtcatat cagaaaaata atccgggtct ttttcaaagt tatcgaatgt tgcgcctgtt
    37441 tttcgccata aagcgatttc tgaatgggcg atctttaaag aagaatctcc atttccttgg
    37501 aatacccagc cgcctatgcc attggtctcg cttccaatcc gtttgttttg gaaatcaaaa
    37561 taggtaggag attctaagtc cacattcaac acatccccac tgatgatgcc ggtcttatct
    37621 tcccctgttt tcccgaccat gactaaataa gttttttcac ctgccgtaaa atttaaatca
    37681 atatctccag ttgtatctac agttgctccg gaatctgcgc gtatatcgat atttgatttt
    37741 tctccagtga ctgtaaaatc agctgaacct ggactttcta gaaaaccaga agcgtcataa
    37801 aattggatcg cttgatttcc ttgattgagt ctgttcacgt aagatttgcc atcacctgta
    37861 tttcttaccc tcaatgagcc tccattggtt actgtcagct tttgcgtacc attctcaaaa
    37921 cgaactgcgc tggaacgccc actctgctta tcgatgtcga taactgatcc ggctccttct
    37981 acgatcaaag aaaggccttt tcctacaaaa cgcatcccat tgttcccggg ttgatcgtca
    38041 tctccagcca ttttgaccga gatttgtcca ccacttgata catggatttg actagaatct
    38101 ccttgcaagc gaaatccgcc cgtagttgct gtattgatgg tgattttccc accattcgat
    38161 acagtgactt tcgatcctga acctctcatt tggatgattc cgcgattgtt gtcattggta
    38221 atattgatct ttgtatttac cgaaaattcg cttcccgaac cactgacttc aaacatgaca
    38281 ttaccaggta gcgtatcgac tgcctctcct tcatcgctaa cgccatctgc ccgatcataa
    38341 tcaagagaga cctttgaacc gcttaagata tggattcctg gcgctgtttt tccactacca
    38401 gtctcatctc ctgaatctag aaaaccgtag aatttcccta gatacggtcc ataaagttga
    38461 ctaccattga tcacattcaa ttgatagctt ctacaaaaat aggctttacc cggagcggca
    38521 ttcagtcctt ccttcactgg ttggacatcc aacacaccgg aaacaccatc taatgtgaca
    38581 gttgcacgag gagcgtaaat cagtcctaat ttggacccat catacaaatc aagtgtcccg
    38641 gtaaatttta tttctcccgt aaaatgatat ctgcttcccc aaaaatcggt catataagca
    38701 atgataatgg catttccatc gatatttggt gtcaatccac ctggatgtgc agctgttcct
    38761 ttaaaattct ggaactcgat cgactgaggt atctcattga tataaatatg gtgtgtgccg
    38821 aaattgtgag cgaatccacc aaaatcaata acgatatctc gtttgatctc taattccctg
    38881 tctaaagtca aaggctgaat cagtcggatc ttagttactg attcgtctgc gagaagctgc
    38941 gccagttctt ctgttgtggc agcttcgctg tacccatccg ctgaaacagc tcttgccagt
    39001 gccgcttcac tgctatcaga cgtttgttca gctacttctg aggtctcact tgtcttattt
    39061 tcttcttgcc ttttattagt agaagcctca ctttgagatt ctgtagtttc agaagtaatc
    39121 gattcttctg aaaaagtagt cttgtcaaaa ctttgattct ctgattgcgt tgattcgttt
    39181 gttttctcta catcggcaaa aacggatgtc accatcaaga gagggaagac aaacaaaatc
    39241 gacgcaatca gcttttttac acattgattc attctccacc atcctctctg acgttttgac
    39301 aattttattt tatcgtttta atctatttcc aattgtactt tcatatttaa aatatgtatt
    39361 tttatggaaa aatttttcta ttaggtgtaa atttcttttt tgtacaaaaa aaaggctgtg
    39421 acaaaagtct tgtcacagtc ccttatcccg aacaagcggt gttctcccgc caactcctcg
    39481 gtcacaacct ccattttaac aataaacctg taaatgtgag ggcaatactt tgattgtcac
    39541 aggtaattta tccccttcat ctccatcaac attcgtcgtc aattcttcct catcagctaa
    39601 tgacacaact aattgctcac aagtaaggta ggctagattt ttcgtagatt catctactcc
    39661 ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa gaagaatctt ttaaataaac
    39721 caaatggagc ttaccatcat ccacttttgc atttggaagt aatgtttcaa aaccgccgac
    39781 tgagttagtt agtcctatca gcaaggtact gctctcaatt tcttccttct ttccatcaac
    39841 ttctacgtga aacgagtagg attgtgtgga agctaactgt ttgattcctg acatgaaata
    39901 agctagtttt ccaaatttcg tcttcttctc cgcatctaca tcattgattg attcagggat
    39961 agagccaatt gcgacgacat tcataaaata gtcgtcattt atttttccaa tatccaatgg
    40021 cttgactgat tcaatcgaaa aatgattgat cgcttcctca ggttctagcg gtatccctaa
    40081 tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat ccgaaatttg gccgatgttc
    40141 ttgttcagcg attccactga ttccttcgtt gactgttcca tctccgccca tgacaaacac
    40201 actatgatac ccttccgtag ccgcttcgcg agtaaagttt tttgcatccc ctgctttttt
    40261 tgtatgcaag acgaccactt catcaaacac cgattccaat tttgctatcg ctaattgctc
    40321 aaattccttt gcttgttcac cgcctgaact ggggtttacc actaataaaa cttttttcat
    40381 cactttcttt cccttctgat tgatttcttt aatataacga tagcataaag aaaaaaatta
    40441 ggctgtgatg aagtactgtt catgaaacat tcatttattt tttgacaaga taaaacatcc
    40501 atactatgaa actgaggaaa attaaaacaa tgatatagga aataaaagaa aatgataggc
    40561 attgtatacc taataaagta aaaatcttag gtggcagata aaaacacaaa aatagcaaga
    40621 gaatgtaata gcgtttcttc cactttttgt tatcagacct tatagcgatc agaggaatac
    40681 caaatgcata actgaaaaac tcttgatgta ctgttaatat gtcacggcct tgaataataa
    40741 atgcgacaat ccccgctata attagtgtgc ctaacaccaa aacggaaaat tgatgaatat
    40801 attttatata caaatgatcc attgtacttc ctgccttttt atttgtgaga aaaacaaatc
    40861 cgtatacgtg aataacttct agaaataaaa gaaatttgca aaaccactcc atttatctac
    40921 ttctacgtgc attttttgat attgttttct ttatttaaga ttatacagaa aaatatctag
    40981 ggtcgctgga ttctaagtaa aaaaaattt
//
LOCUS       DAYYFD010000012        40939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000012 DAYYFD010000000
VERSION     DAYYFD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.012"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1838)
                     /locus_tag="ACHA25_000594"
     CDS             complement(<1..1838)
                     /locus_tag="ACHA25_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8761922.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
     gene            complement(1835..2575)
                     /locus_tag="ACHA25_000595"
     CDS             complement(1835..2575)
                     /locus_tag="ACHA25_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8761923.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(2565..5354)
                     /locus_tag="ACHA25_000596"
                     /pseudo
     CDS             complement(2565..5354)
                     /locus_tag="ACHA25_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            complement(5596..5937)
                     /locus_tag="ACHA25_000597"
     CDS             complement(5596..5937)
                     /locus_tag="ACHA25_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761924.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            complement(5937..6545)
                     /locus_tag="ACHA25_000598"
     CDS             complement(5937..6545)
                     /locus_tag="ACHA25_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8761925.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            complement(6546..6923)
                     /locus_tag="ACHA25_000599"
     CDS             complement(6546..6923)
                     /locus_tag="ACHA25_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8761926.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            complement(6925..7320)
                     /locus_tag="ACHA25_000600"
     CDS             complement(6925..7320)
                     /locus_tag="ACHA25_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8761927.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            complement(7313..7684)
                     /locus_tag="ACHA25_000601"
     CDS             complement(7313..7684)
                     /locus_tag="ACHA25_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761928.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            complement(7684..8025)
                     /locus_tag="ACHA25_000602"
     CDS             complement(7684..8025)
                     /locus_tag="ACHA25_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761929.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            complement(8037..8252)
                     /locus_tag="ACHA25_000603"
     CDS             complement(8037..8252)
                     /locus_tag="ACHA25_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761930.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            complement(8275..9165)
                     /locus_tag="ACHA25_000604"
     CDS             complement(8275..9165)
                     /locus_tag="ACHA25_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8761931.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            complement(9180..9803)
                     /locus_tag="ACHA25_000605"
     CDS             complement(9180..9803)
                     /locus_tag="ACHA25_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8761932.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            complement(9930..10247)
                     /locus_tag="ACHA25_000606"
     CDS             complement(9930..10247)
                     /locus_tag="ACHA25_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761933.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            complement(10249..11301)
                     /locus_tag="ACHA25_000607"
     CDS             complement(10249..11301)
                     /locus_tag="ACHA25_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8761934.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            complement(11219..12748)
                     /locus_tag="ACHA25_000608"
     CDS             complement(11219..12748)
                     /locus_tag="ACHA25_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8761935.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            complement(12760..14175)
                     /locus_tag="ACHA25_000609"
     CDS             complement(12760..14175)
                     /locus_tag="ACHA25_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM8761936.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            complement(14168..15001)
                     /gene="terS"
                     /locus_tag="ACHA25_000610"
     CDS             complement(14168..15001)
                     /gene="terS"
                     /locus_tag="ACHA25_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8761937.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            complement(15160..15390)
                     /locus_tag="ACHA25_000611"
     CDS             complement(15160..15390)
                     /locus_tag="ACHA25_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761938.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(15800..15937)
                     /locus_tag="ACHA25_000612"
     CDS             complement(15800..15937)
                     /locus_tag="ACHA25_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761939.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            complement(16198..16614)
                     /locus_tag="ACHA25_000613"
     CDS             complement(16198..16614)
                     /locus_tag="ACHA25_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8761940.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            complement(16690..16986)
                     /locus_tag="ACHA25_000614"
     CDS             complement(16690..16986)
                     /locus_tag="ACHA25_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761941.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            complement(16983..17186)
                     /locus_tag="ACHA25_000615"
     CDS             complement(16983..17186)
                     /locus_tag="ACHA25_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8761942.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            complement(17183..17569)
                     /locus_tag="ACHA25_000616"
     CDS             complement(17183..17569)
                     /locus_tag="ACHA25_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761943.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            complement(17600..17794)
                     /locus_tag="ACHA25_000617"
     CDS             complement(17600..17794)
                     /locus_tag="ACHA25_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761944.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            complement(17900..18352)
                     /locus_tag="ACHA25_000618"
     CDS             complement(17900..18352)
                     /locus_tag="ACHA25_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761945.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            complement(18378..18839)
                     /locus_tag="ACHA25_000619"
     CDS             complement(18378..18839)
                     /locus_tag="ACHA25_000619"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8761946.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            complement(19040..19891)
                     /locus_tag="ACHA25_000620"
     CDS             complement(19040..19891)
                     /locus_tag="ACHA25_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8761947.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            complement(19906..20706)
                     /locus_tag="ACHA25_000621"
     CDS             complement(19906..20706)
                     /locus_tag="ACHA25_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8761948.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(20749..21639)
                     /locus_tag="ACHA25_000622"
     CDS             complement(20749..21639)
                     /locus_tag="ACHA25_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8761949.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(21641..22582)
                     /locus_tag="ACHA25_000623"
     CDS             complement(21641..22582)
                     /locus_tag="ACHA25_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8761950.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            complement(22815..23150)
                     /locus_tag="ACHA25_000624"
     CDS             complement(22815..23150)
                     /locus_tag="ACHA25_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761951.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            complement(23209..23337)
                     /locus_tag="ACHA25_000625"
     CDS             complement(23209..23337)
                     /locus_tag="ACHA25_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761952.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            complement(23476..23664)
                     /locus_tag="ACHA25_000626"
     CDS             complement(23476..23664)
                     /locus_tag="ACHA25_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761953.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            23742..23966
                     /locus_tag="ACHA25_000627"
     CDS             23742..23966
                     /locus_tag="ACHA25_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8761954.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(23963..24118)
                     /locus_tag="ACHA25_000628"
     CDS             complement(23963..24118)
                     /locus_tag="ACHA25_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761955.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(24258..24470)
                     /locus_tag="ACHA25_000629"
     CDS             complement(24258..24470)
                     /locus_tag="ACHA25_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761956.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(24473..24685)
                     /locus_tag="ACHA25_000630"
     CDS             complement(24473..24685)
                     /locus_tag="ACHA25_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8761957.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            24960..25370
                     /locus_tag="ACHA25_000631"
     CDS             24960..25370
                     /locus_tag="ACHA25_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8761958.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            25383..25835
                     /locus_tag="ACHA25_000632"
     CDS             25383..25835
                     /locus_tag="ACHA25_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8761959.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            25926..26138
                     /locus_tag="ACHA25_000633"
     CDS             25926..26138
                     /locus_tag="ACHA25_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HFM8761960.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            26245..27432
                     /locus_tag="ACHA25_000634"
     CDS             26245..27432
                     /locus_tag="ACHA25_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8761961.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(28100..28714)
                     /locus_tag="ACHA25_000635"
     CDS             complement(28100..28714)
                     /locus_tag="ACHA25_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8761962.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29320..29697)
                     /locus_tag="ACHA25_000636"
     CDS             complement(29320..29697)
                     /locus_tag="ACHA25_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761963.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(29997..30878)
                     /gene="galU"
                     /locus_tag="ACHA25_000637"
     CDS             complement(29997..30878)
                     /gene="galU"
                     /locus_tag="ACHA25_000637"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8761964.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(30921..31940)
                     /locus_tag="ACHA25_000638"
     CDS             complement(30921..31940)
                     /locus_tag="ACHA25_000638"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8761965.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(32573..33577)
                     /locus_tag="ACHA25_000639"
     CDS             complement(32573..33577)
                     /locus_tag="ACHA25_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8761966.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(33590..34639)
                     /locus_tag="ACHA25_000640"
     CDS             complement(33590..34639)
                     /locus_tag="ACHA25_000640"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8761967.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(34656..35477)
                     /locus_tag="ACHA25_000641"
     CDS             complement(34656..35477)
                     /locus_tag="ACHA25_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8761968.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(35464..36243)
                     /locus_tag="ACHA25_000642"
     CDS             complement(35464..36243)
                     /locus_tag="ACHA25_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8761969.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(36260..36730)
                     /locus_tag="ACHA25_000643"
     CDS             complement(36260..36730)
                     /locus_tag="ACHA25_000643"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8761970.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(36742..37161)
                     /locus_tag="ACHA25_000644"
     CDS             complement(36742..37161)
                     /locus_tag="ACHA25_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8761971.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(37242..40019)
                     /locus_tag="ACHA25_000645"
     CDS             complement(37242..40019)
                     /locus_tag="ACHA25_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8761972.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            40210..40434
                     /locus_tag="ACHA25_000646"
     CDS             40210..40434
                     /locus_tag="ACHA25_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761973.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT        12002 a         8348 c         6192 g        14397 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcac taggataccc accagtagat
       61 tctccacctc ccgatggacc accaccttgt ccaggaatta tttcttttcc tttaactgta
      121 agttttcctt gtacatctaa gtctccaaaa taaattgctt taccatttcc taataaaact
      181 aatccttttc ctactttagg agaaatcaaa atatatttgc cgtctccatt tgtacgaata
      241 actaaagaat tatcttcaat aggggttgga gtagaagccc caggaaaagg attaccaaca
      301 gaatcagttg ttccaatcgt tccaattgaa cccttagaat tccaaaattc cattcctttt
      361 ttagttaact ccattatttt ctttttgttg ttccaaattt ggagtagtcc attaactaat
      421 ttcaatacat ctccagtctt gttaaaagaa ttttgaaaga tatctgcttt gattaatcca
      481 gtttgtataa aattggcatt aaatatacta tctaatgtcc aagctgaatt aaaaggcccc
      541 cgccatcctt tcttagaaaa agcaatccca tttttattca ttcttagtac ttctcttgcc
      601 ttttctaaat ctggattatc cataataaag atgttggaag gtttttcttt tggccataaa
      661 actacatatc ctcctgcact gccttgacct gtaatcattg aagaaacata atcattaaaa
      721 tcactcatat aattattcgt tgcataatct tttaatttat cctgaatagt tacagcttgt
      781 tgctgataaa aagcaacttg aatgtctcct gcttctaatt tcaaagtttt ttcagataaa
      841 gaatcgtatt gtagtccact aacctttgat tcaagataaa tattatattt tttatgatat
      901 attttaaatg tatcaaacag accataattt ctaatcttcg caaattcttt cgcttcttca
      961 ctatcagtca acttatcaaa ttcaaccgta atagaaactt taggcttatc acagccagga
     1021 ttgatcgttt taaagtaatt tttagctatt ttatttaagc tcttaatgtc ttttactcct
     1081 tgttcttctg taaactgaac atgttctgta tagacatcag gatagttatt aatataggca
     1141 ctatctactg gcgaaccata aattcgatta gtcgtaccta cttcactttg aggatcagca
     1201 tatggaataa ttcttgtttt tatacctgtc caatctaatt tgaccttcaa accagacata
     1261 tcttttccat accgaatcgt tccaacatta ttgcgacctc ttcgctttaa caaagaaagc
     1321 ttaaatggtt cacgtttaat ttctccgccc caatattgaa gcaatgatcc ttgttcacca
     1381 gcaatacaat ttaaaacatt tctcgcttca aagattgtgc tagaaacagc cgtaatatca
     1441 gaataaagtc gtatgtcaga ttttttatcc attttagttt caataattga tatcgcttct
     1501 tgaccagttt tagagtccac ttccacaaga gtgactaccc gtcttccaag tcgatttgta
     1561 cgactctgag cataaatagt tactgtattt aaaaaagtat ctatatcttt atcatcaata
     1621 aaaaagatat gatactcttc ttgatcgttt ggctttgctt ttatttgata gtcattttca
     1681 aaatattcat caaatctagt tcctaaagga taatccaact ctaattcata ttttccatta
     1741 gctacttcat atatttcaca tcttgtacaa tcctttaaaa ttcctaagcc attcgttgag
     1801 aaatctgttt cagtaggact atatattctt ggtttcatac ttttctccac cacctaggca
     1861 ttatttcaaa tgaatgaata tttttcgtcc atttaatttc attttttcca ggatataaaa
     1921 atggaaaatc taaaaataag gtgacctgat cctgatgttc taaattacca tctaattttc
     1981 gataagcctc ttctaattta gaatctataa ataattctct gtccaacgat ttcaaatcat
     2041 atttatcctt attaatataa aaagaagcat ctccagaacc actcaattta ataagcggtt
     2101 ttgaagaata cttctctggg ttataaattt caaaagtttc tgtttgtcga atagtaaatc
     2161 taccattgta attttcctta aatggtcgaa tactcactgt aaattcaaaa gggacaatat
     2221 ttcctgtttt tcttgttcct ttaaattcag gtccttctat tactacagct tgataaatat
     2281 attgttcatc atagtaaaga ataaaatcac tataagacga catatctaac cattcagtga
     2341 ttcgatcttc ccactcctgc accatatcaa ttgaaggtgc tttatagtaa cactcaatct
     2401 ttctagtcac atttttgtaa tagcctttat caataatgat agaatcattt ccttctcgct
     2461 ctctcaattc aattactcga ctagctgaaa cagaagcagg cctgttttga atatacacat
     2521 taaattcaga agaataatgt tgattaataa aaaattgtcc tctcttaagt tgcatataat
     2581 gttcctccta ctgcaccagc atctcgtttc atttgtcttg tcaattctgt tttaattttt
     2641 gtagtaatct ttcgaaccat tgaatcaggt aaatctccat aaacatttaa atgtaaatga
     2701 atttcttttg tttcactagc agatatattt tgctttgttt gtgtaattgg taggtgactt
     2761 attccattta ctacaggttg aacagacggt gtttttacta aatttgtcat ggtttgatct
     2821 aacttacttt gctctttatc aataccaacg ataatacctt gaacaatatt tttaccaacc
     2881 atatcacgca tccatcttga tggagaatga atatctaaag ctcctttaat ccaatttttg
     2941 atattacctg caataccttt gatagtatct ttcaaagcat tccacttttc ttttacacca
     3001 tttataagtc catcaatgat gttcttgcca atttcaaata aattgacttc ccttaacgaa
     3061 ttaaagattt cttttacccg attaatagca tttgaaacac cattttttag atttgtccat
     3121 gcattttcag cagaattaac aattccagta acaatattcc agaaagagtc cttaatgtta
     3181 ttccatgtat taatcatggt attttttata gaaatccacg tattgtatgc cgtatcttta
     3241 atatttttcc aagtatcttt gaaaaactgt ttaatattat tccaagtagt aatggcatta
     3301 tattttaaat cgatccaagt ttgaataata gaaaacttca attcaatcca tttctgaatt
     3361 gcaaaatatt ttatatcaat ccaaagatta ataaaaaaat actttatgtt taaccaaatc
     3421 gatttagctt gatttaccac ttcattccaa atatttatca atgtaagctt gaaccctgtc
     3481 caaatattga gtgcagcaaa ataaatattt gtaaaataac ttacaaatat attctttata
     3541 gattcccaaa tatttatggc actttcttta atattattcc aaacagcaat catattattt
     3601 tttgtttctt cccatccacc agaaatcatg gaggtaataa acaaaaccgg cgcaagtata
     3661 acatttttta aaatctcaaa gacgttttta ccaatctcaa ctaaattatt ccataaggtc
     3721 tctagataaa aagtaacatg taaaaatgca ttctttatac ctgtaatcaa tggaccagca
     3781 actgacataa tagaaccttt gattccattc catttttctc tggctgaatc ttttatactt
     3841 tgccaagtat cagaaaacca tttcttcata ttagaccatg cattttttac actatcgact
     3901 gcatttcttg aagtctctac tgttttatca aataaacctg tggccccatc cttaatacct
     3961 ttccaggtgt tagaaaacca ttcctttgtt cctgaccaag catctttgac accctcaact
     4021 gcatttgatg ccatttattt agcagaattc caagtatcaa ttccccagtc ctttatattt
     4081 tttaatacac caattacgga atctttcaca tcattccaaa tagatataac cttgtttcta
     4141 agctcttcat tggttgccat aaagtaaccg aaaacagcta ttgcaccaac gactgcacca
     4201 ataattaaaa caaatgaatt agcagcagct attgccccca taatctttaa tgaatttcca
     4261 acgccaatta tggcattctt aaaattattg aagcttttta ttattgctgg tataccttta
     4321 agttgaaaca tgaacgctcc aaaagcggta gctgcaggaa caagaaaggg catcatgaat
     4381 ttcaaagcag aaccaaattt ttgaatttcg ttaaataaat tacttaaaaa tgatactgct
     4441 gtaggaatat ttgcagcaat tacttttaag aacgattcca ttgaactgcc cactttatca
     4501 ataattccct taaatccacc taaatctgaa tctactaatg ctttatttaa tccttctatt
     4561 acctcaccaa caccacgtgt aacagctgtt tttgcatttt gtatagaggt tttaattccc
     4621 ttagtagaat ctttagcgat ttgacttaat gatttaagtc ctccgccacc tttggtatcc
     4681 atttctatta attgattttg gaattcttca acagaaatct taccttgaga taaaccttct
     4741 ttcagatctc ccattgtaat tcccatcttt ttagcaatag cagaaagagt aggtccaagt
     4801 tgagcgttga tcattgagtt ccaagtttgt gcgtctactt ttccgtttga gaaactttga
     4861 gataattgaa taacagcttc attaacttga tcagttgaac caccgaaacc aagaattcca
     4921 tcatttaaag ctttaaatat ttgagttgaa cgagttaaat cacctgtaga agaagcaaga
     4981 agttgaacat gacttatcgc atcattcaaa gcagtaggaa gtccttgaat accttgagat
     5041 aaaagcccat tctttccgat attcttcata atttctgaat tactaaatcc catattttgg
     5101 aaatttcgta gagcgttatt catcgtatct actcgatcaa ccgctccact tatggaaccc
     5161 ttgattagat caaagccagc tcctacgatt ctagttactc cgctagctag aaaacttcca
     5221 gcaaatattt tccaaatact acctaatgaa ctgccactat ttctactttt tctttcgatt
     5281 gtttcatcaa aagagttcaa ctttttgaca gcattattca taccggcagt aaaaccagat
     5341 tcatctagta tcatcttcag aattaggtca tcgttattca aagtatcacc tcctaaaatt
     5401 gggtgaagtt atcgtagtac tcaacatcct catgctcttt tactgcatct cgaaaagcaa
     5461 aaagacgcat taactcgtca aagtcagtac gttcaatttc tggtaacgtc caacctgctt
     5521 cgagtaattg cgtctttaaa tctaattctc gataagtaat cgaatgttta aaaaaaggat
     5581 atttgagagc ttcgcttact tttttttcgt ttctgtataa gtttcatcaa agcctgcagt
     5641 aacagatttt aataacttac ctgtcaacga agcaatttct cgagcatcga tcccttgacg
     5701 ataatcttca ccagtaaact gtccttcaaa caaaatatct gcaataaatg aataagctcg
     5761 acttaatgct tctgatactg attttccatc tacagcattt tgcattccct ccattacttc
     5821 tgctgcttct tctacgactg ggcctggtag aaattcagca gatttaaatt gtacttgctc
     5881 atatttacct tcttcatctt ttttcgttaa tttaatcgtt gtttgatatt ttgacattat
     5941 tctgtacctc ctgaaagttc tgcttcttca attggaattg ctacttttgt aaaccaattt
     6001 ccgatcattg ctttatccac gccttcatca tcttcatcaa ctgaatacat atatccaagt
     6061 cctggtacat ctacaaatga acccttccaa gttggatgag taaaacttac tttacttcct
     6121 tcaatcgttg atgtttcatc tgaatctaga gcaaactgtc ctttataaaa gaccgtataa
     6181 cggtatttcc cattcgattt tttacgtcta tatgcaaacg caccatcttg cgcaatatct
     6241 tctccagaac gcaaggcacc acctttaaca attttccctc cagtaattgt agataaaact
     6301 ttatgagtat atccatttgc ttccagttca acttctgcac caccaaaagc tgtaaattga
     6361 tcttgaacca ctgaatcccc ataatcttgt gttgtttcat aattagctgt tggtttgata
     6421 cttactgctg ttcccattgg caacgcagta ccatataccg gaaaattccc tgtctcatct
     6481 tccaaaggaa accaagtagg tttttcgaca gaaataattc cggttttact tcttttttct
     6541 cccatttatc tttcactcca ttcaatatat tgtggaaatt ctaatgtaaa acgaatatgt
     6601 tgaacaccat ctgcttcatc aggtaaatag gcttttggaa ataatatttg ctcatcgatt
     6661 gtaatcgtat taaaaaaagt cccacaacgt gagactaatt cattaattaa atttctatta
     6721 ggtttattat caatcaatgc aatatcaatt aagaaagaaa cattttgaat atcaattcct
     6781 acattttcag ttccagattc cattactgat aaaacgaaat aaaaatcttt agttgactgc
     6841 ataactgaat caagatatat tgtcccatca ggatagattt cttttaattt gctactgatt
     6901 gcagcaatga tttcatcttt catgttactt tcctttctta acgattttta ttgccatttg
     6961 tttaaatctt cgaggaatat aagacatatt tgcaagattc gttgcacgag ttaacatgaa
     7021 tttccctttg acaaagccac catttttagt cctatgacct tcttctacat atttaaaata
     7081 atgttcattg tttataactg ctccaacaat tcgaccgctt gatagttttc tagcttttac
     7141 tacacgatat cctcgcctta aattacctga ttttacagga gttaatggtt tagctaaact
     7201 aacgacttta ttcattgaat cgttaacgaa ggatattcct tctttttgag caattacagt
     7261 catattttta aaattctcaa tgatcttttc tgcatttgat tcatatttga gattacccat
     7321 ttttctcact tcctatcaaa ttcacttcac aatgactagg atagtaaaaa ggcttagtag
     7381 caaatagaga aaaaattaag ccactggcct tttgagttat tgttattcga tctccttttt
     7441 ttagtttaat cttaggatga acaaataatt tatatgtgtc agttgaaaca tttaccatct
     7501 ctccatcttc tatgactgct agtccatcga tttgaccttg tgaaagagcg caagaaatag
     7561 gattatcata tacttttttg taatcttgaa tagtaatatt tgtattagaa tcttcaatgt
     7621 ccgtcagtct ttcaataata caactatctt cataagtagt ttctaaaata tctgcttcat
     7681 tcattaaaaa aactccaatc ctccacaacc aataactctt cgtatcaaat caccatagcc
     7741 tagaagtaac tcactaacat catttgttgt tgaagcataa ctaatcgttg tatcgccacg
     7801 tctaactgaa gatactgttt tttctgattc attttttatt attttataca acacttcact
     7861 aatcacgctt tttaactttt cccatgaaat atcattttta caagtgttgt atgattcaat
     7921 ttctagcaaa attatttcta ataaattagt tatgcgttct tcactcaaat ctggaaaatc
     7981 ttcttttgta gactcaatga tttccgtttt tagcgattca tccatcagat cacttcctaa
     8041 atttcattaa catcaattgc atcttccaaa attgcaattg cttctttatc atctatagaa
     8101 atgataaatt cgttatttga atctgctgtg ataaatctac gagttttagg atgaacaaac
     8161 ccaacaaaat ttttagtttt acctactcta tatttcacga cttcatcttt tttagtttca
     8221 tcttttttta ccactgtaat tcctcctatc aaaaaaggga gtactaactc ccttttattc
     8281 agactttaaa tttaaaattg ccccagagtt tgaagcattg tattcaagag agtattcacc
     8341 tacaagaccg atacgtctag aatccgttgt ttttgcaagt tcttctgctc tccattcacg
     8401 taatggacgt agtttcacat aattagtatc gattgcggca attgttcctt ttggcaatga
     8461 tggttctagt agagcaatac ctgtaccgta attagacacg atatttccaa tttgcaatcc
     8521 aaaagttact ctttccccga actgaacaat ttttgttgat tttccatcta actcatcagt
     8581 cattaactcc tgcatatctg gagaaatcaa gcatagtttt tctcccatat aacctttttc
     8641 atacatcaat ttaaataaag tatcgatatc ttttcttgtt actgcacccg cagtggctgt
     8701 ttctgcctta tttgctgaat tgatcaagtt caaaatccca ttcattcgac gacctttaga
     8761 accattttca tcagctttta caccagtaat caattttcgg ttcaaatcaa ttttcatttc
     8821 cataccacgt agtgctactt gattagttaa ttcatttccg actccattca cattaatagc
     8881 atctaatgta ccagatacag aggttgattt tctaaaaatt tcagtatagt tgttaaacca
     8941 agttcgacct gattcagcat ctggatattc tccgccctca agtttttcag aagaatcatc
     9001 gttgttgata tcatattcac gccatttaat ttctgtcgag tttgctggct ctgtttttcc
     9061 agcaccaagt aaatagctta aaaaaggtgt atttggtact tgcatagcat taatcgctgg
     9121 tgaaatatcc aaatactcta aattatttaa agatgttttt ttcataattt ttcactctcc
     9181 tagttaaatt gttgaagaat ttgtcctaat tgttcttctg gatttgttaa tgtttcattg
     9241 ttctgctgaa cctggttatt cgattgttga gttccaccaa atgcagactt catttgtagt
     9301 tcttttaatg catctgcatg tttctcatta attgcattca aaacacttgt aaaaccttca
     9361 acagcattct tagtgaaatc tgtatccgaa ctaacaagat tatttagcat aaattgagaa
     9421 atagactctt ttaactctcc atctagttct aatccagcaa tttgttctgc aacaaatgct
     9481 ttattatcac ttgtcacacg caacgctttt tctgcttcaa attcagcttg tagtttttcc
     9541 agttgaattt gctcaggtga cttgtttttc ttagattcct catattcctt gatggtatcc
     9601 tgtttaattt tatctaagtt attttgtttc caagcttcca attgtttgtc ggcaacactt
     9661 tgagattgtg actgaataaa tttttgagct tcttcattgg attccacaaa agatttgaag
     9721 tcatcaaaag aaaacttttg atcgtcacct tcggcaaagt attgcaaatt cattggcatt
     9781 aatgatttgt gtttcatgct cattctcctt tcgccccacg attcgttttc acgccccgca
     9841 ttgctttgag ttttaatagt tgcgccccac cattcaacca agcccagtat tgcgctagtt
     9901 taacgtcatt tcggacaaaa taaaaaagcc taattactag actttccttc tttgtagtca
     9961 gttctaacaa caatacatcc catcttttca taccaatcaa ctgtttcttt taaatttggt
    10021 aatgaatgag ataataattg aatcgttaaa ttaacggtat tcttatcaac tggagtatta
    10081 tcttgataat taacagtttg atcgccaata accacgtagg cataattgcc attccaatgg
    10141 tctctcaagc cattaggatg gttctcagac tcaatagctg ttttgtaggc ttctgcaata
    10201 tcagcattta cattaattgt tagtactgct tcaaaaaaat ctttcattct acttcctccg
    10261 tttcaaatta attccaaaag aatcatgtgc aaactcatct aataaatcgc caaataaacg
    10321 gtcatattct tcatcaatat cctttcctaa ttttggtaaa ttaggaacat ccgtacatct
    10381 acaacgacta tggaaaggtg ctcgattttc acctatgaca gcatctttca gtttataggg
    10441 attcttactt gcctttcctc cacaaatccg gcaaactctc tcatcttttg cagtcaatac
    10501 gttgtattcc tcaatacctg tttctaaata tgatttttca ataccatctt gtgcaaattt
    10561 tgcatactcc gttcgaacga gattttctat cgctttgtta tactttgatt cttctaactt
    10621 aaacatgtca caaatttctg agtctaatct catggtgttt aatgcgtgta taacgccttc
    10681 tccactagca acacttttta caatagcatt agagagtttc tgttcaagcg atgaaatatt
    10741 tccccaaagt cttcttgaaa atgtctttcc tgaccatgga tagttcaaat aatgtttaat
    10801 ttcattttct ggtaaataat ttggtatatc aacgtttaaa agttgaacaa aaacattagc
    10861 attagaagaa tatgttcttt gaagaatttc ctctaagcga tcagaaaaat attgattcac
    10921 atctgcatcc atcgcatatt ctgcaagaat tttaaataca tctgatcgta tttgtaataa
    10981 gcgattgact tttgcatagt caaatggtgg aaaaaattca tcgataaact ctttgtattt
    11041 tgcatcatac atttttaatg acttataatt tttttccaca tattcacgat attttttctg
    11101 attacttgta ctgtaaaact ccatcatttc tggataagtg atattgtgta agtcagcttg
    11161 tgataatagt tttgcttgta tctctttcag tgcttcagga aatacactag ttaatttttt
    11221 aagtgtctga ttttcttgtt tgagccttgc ttgatcctcc agttctcgac gtttggtcca
    11281 atacttcgtt tctatcgtca ctattgccac ctccgccaaa attgtattca ctatctggat
    11341 aagcacttcc actttccaat tccatcattt cattttcata atcaacatct gttacaaatg
    11401 gaatttggct ctgaatagtt cgtttggata cgtatggtgc aagtttaggt aaagcttcag
    11461 ctaaataact taaatcagtt ggcaaagatc tagagaaagt aaaaataatt ttttcaggct
    11521 caatttctac tttgtcatga aacttaagaa aagcagcaat tgtttctgcg cattctttca
    11581 atccttctct aaaatattgc tctttagtat ttgttttcgc ttctaggcta atgatttgcc
    11641 acttgcgagc ttctccagaa ctgtttgatt taaacacctc atcgttaaaa tcgattgctt
    11701 tacaaatggt gtaaaactgt tttttcaata gatccatatg atattcattg aaatcttttg
    11761 ctaatttttt tgtgacatat tcagcttttg cagtagggtc ttttaagttg ataatcccta
    11821 gctgattcat catttcttta gcagtgcctt tgctcattgt tgtgccagta acaagcatgt
    11881 acgcaagctt aaattgttcg atttcgtttt gttgatcaga caaagcacga tcaattgcat
    11941 ctccaatttc ctcagctact tcaaaatcac aataacgatt cgtattattt ttaaattctg
    12001 ccaaggtaat cgtccccaaa gggttatcgg tctcatcaat ttttttgaac gttccgcttg
    12061 taacaaaatt caaatcacca tagcaagcat atgtcagaat tttatttctt gtcataactt
    12121 tcatttcttg aaaaaatttt ttctggtaag agtcatactt ttctttgata tagattccgg
    12181 catttccata tctttctgct ctccaaggtt caatattact tgctcttaac ttccaaccat
    12241 catcttcttc cacaggctct aacaatcgga aagctactcc acaagctcct tgaaaagttg
    12301 cagtttcaga atcaagcatt gcgaatctca tggaatcaag attgcttgtt aatttgttaa
    12361 aagcttctgg aactgttgga agttctatat cattttttaa gaatttatct tttaatcttt
    12421 gaatgagtgt tcttttttgt tccgatacat cgtaatccca tttgataggt ataccagtaa
    12481 aatgattgac tgcttggtct acgacaattg aatacatacc agcatgaagt ttattattaa
    12541 cttttacaat atcagtgtta ggctttggtc tattgtctat ttcattcttt tcgctagtat
    12601 aagctagata ttttctttcg cgatcagcaa aaaatggttt catatattct ataaaaccat
    12661 tcggatcaaa cattccatct tcaatttgtg ttgcatattt aacacgtagc ttcttatatc
    12721 tctctaaaga taatactgta ctaatcaaaa actcacctcc taaaattgaa taaattgata
    12781 gtgacttggt ggctcataaa acgctaaagc cagtgcgtca gcaatatcgg gactaccaat
    12841 atttcgcttt ttcatatcat ctttactttc taaacgtata cgacttctac ttgtcatttt
    12901 gaatttgcga gtacttaatt ctttgattaa cgaactatca gaaggcaatt caataacagg
    12961 ctgttctccg ttaagatttg ctgtcatatt ttcttctaac atttccttga tgtttcccca
    13021 tagttgagta cctaaattat cgtaaaaatc gtcttctgat gttgaaccgt tattcactcc
    13081 aaacacctca aaaggataat gcttgtcttc tataagttct tctaggcggt cggttacacc
    13141 acctccgaca ccagtgtcat cgactttaat catcacttta tcaatactcg gatattgact
    13201 cattagattc ttggccatgt tgatgacata tcctgttgtt tccatagtgc tacgttttga
    13261 atacttctca tactccaatg ccctggtagc aattctagga aagagaatcg tagaatcatc
    13321 accatatcga gctacgtcaa caccaatatg agcaaccgtt gttttattga ctaaagaatc
    13381 actaatttgt ttttctgtag ccaattcaac cgtttcaaga ctgataaatg aatccaacgc
    13441 acctttggga aattctccaa aaatacggac acgagcaaca tcactttctt ttccatattt
    13501 tttaagaatc atttctatat tgtctttgtt tgtacgttta ctatcatagc ttgatacttt
    13561 atgaactctg tatttatcac ggtctgaatt gtgggaatcg taaaagacac cttcaatatt
    13621 attagggttt ccacacatca acagcttatt atcaaaacct gatagcgtac caagaatagc
    13681 ttccataatt ggatcagaca caccagaagc ttcatctacc acaatcaaca tatggtcctc
    13741 gtgaaaacct tgcatatttt ctggtttagt cgctgttcta gccgtagcaa accaacgctc
    13801 tgaatcacca accatataaa ttttggtctt tgtccacttc agtaagtttt tgatcaagct
    13861 atcatttaac catttagcta cctctgccca aagtacatcg taaagttgct tcattgtcgg
    13921 agctgttgct attactttag cgtagggtcg acacgttaag aaccaaagta ttgctcctgc
    13981 ttctaatgct gtttttccaa ctccttgacc agaacgaaca gaaacctttg aaaattcagc
    14041 taaatcattt aaaacatttt cttgccattc atcaggattc aaatgcaaaa tatcttggca
    14101 aaaagctact ggtttatcat agtaataatc aatggcagaa ccaatatcag caaaaggaat
    14161 aaattcatta ttcattttct ttcaccgccc gtttgtttgc ggcatttaaa actgcctgtt
    14221 tccattcttc tatttcttca cctgaattat ttccgccaat ttcatttgtc tcagcttgga
    14281 tcttatcagt ctgagcttta attaaatcag ttctgtattc attcatataa agttcattca
    14341 tttgcttgat ggctttagct aactggttgc taatccgagt caatgaatct tcaattgaaa
    14401 gaatatcatc aattttgcga tgtttttttc tgctaatctg cacatcttgc ataacctcac
    14461 gcttgatttc tagcttttta ccattttttt caatcggagt tttaatcttt cttagctgct
    14521 gcaatcgttc gacttcttca tcatttagtc cagcttcagc ttgttggatt cttttcatca
    14581 ttcgaagttg tcttatttta agaagtcgta tttcttcgga caaagcaaaa gaaggatcat
    14641 cattcaaact agaatagatg tccttctcat tatcggttaa cgtgtcaaaa aatattgttt
    14701 catactcacc agttttaaga gcattcttat ttctctttgg cggtgatgct ctgctgtttc
    14761 ctttgttacc tttagcattt tgattaccaa taggagcgcc accttgatta gtaacgttac
    14821 ttttgccatt ggtaacgtta cttttcaatt cagcgctcca tttgtcttgc gatttccatt
    14881 ttctaacctg agaatctgaa acatttaatt cagatgcaat ttcctttaac tgcttttctc
    14941 cgttggattc taaccaaatt cttttggctt catcacgtct tggatcacgt tgtcttgcca
    15001 ttcaatacac accacctcgc aatcttctta ggttgagttt tgttttctaa ttttcaatat
    15061 cttttagcat taaatcagct tcaatcaata tctttaaatc ggaaactttg tctaacttga
    15121 tttgtcctga ttggagattt ttaagccatt tcttgcattc taatccctca aattcttgtt
    15181 aatattattt tgaatgtttg gttcatcata gaaaccatga ccgcaataaa gaagtttgca
    15241 tttatcaatc tcttttggcg tagcttctct ggtcatttca acaatggagt acttcttttt
    15301 gatttggaca gaacgcacaa cacgcactga acaatcatca atggttcgag gatattcatt
    15361 agttagcgat atataccagt agtttctcat tatgtagcct cctttatgta aataaaaaga
    15421 ccactcaaag agtgatctaa tatgtaatag caacctacac acaggcggtt atcctgttcc
    15481 tccctagggc tcgcaccaca cacgtactag tcctttctag ttttgtacaa ggtcccgata
    15541 gtgcgccata cgcttcctac ctccaccttt gcgtcttcta tttccgccac agtgacacta
    15601 taaaattatt ctcggctgct actatttttt attttgtcca tttttaaatc caatcatata
    15661 gacattaaga cagagcgcaa aaattgaaat tattaacgcc gtcatttcta tttacccacc
    15721 ttttttaatt atttagttaa gttatatttt tctgatactt ggtatgattt tatgagtgca
    15781 gctgctaaaa aatatactgc taagaaactt ggattttaaa attagcaatt tcatctaaag
    15841 tttcttgtaa ctgttcgact tggtcactag ccttgttgag cagttctttt agttcagtca
    15901 aatctaattt gacagttact ttgatttctt tatccattcg tttttcctcc gatgcataaa
    15961 ttaatagaca acaacggatg atagataata agaacaattt agaaggagtt gaaattcaca
    16021 tccttattct taatatttcc gttgctgtct atcgaagctt aagttaaaac gatgagggag
    16081 atttcctccc ttacatttta ttttgtctca gacctatcac taatctttcg acactaccat
    16141 aatatcacgt taaaccgctc aaaaacccta cactatccct acaaaaaccc tacaaaatca
    16201 acgatactga actaacacgc cttttttgta tgcttctgca aattcgatta atgcgatgga
    16261 tttcaacttc tctacattct tctctccgta tcctcgtatc aattgcccta tttcataatt
    16321 agagtgcttg tttacgtcac agaagctgta gtagagtatc tgacggctaa tcagactaag
    16381 agccatcaaa gccgctagta ttgcgtctct ctccgcttct atatccatca tctgaatgat
    16441 cgcgtcttct gccttattgc cgtgcttcgg tgccttcggc atatccgtaa taatcggcga
    16501 cttaatatct atcaaagagc gacctgccat ccgctccaaa cgccgaaagt tcttcagcac
    16561 atctctcgca ttacatcttg tctgtttgaa atctacctct cgtaacaatt gcatcaagtc
    16621 aaaccgctcc tttatgtgat ataataaatg tgttggattt attgaatcag tcggagcgat
    16681 ccggcttttt tatttgtcat tgattagttc catatccacc aaacgagcca ctgctaaatt
    16741 atctttgctt ttcgctaacc acttatcgca ttccatcgtg ttttcaatgt gaatgattgc
    16801 tgaatgatta tagacatgct ctacatatcc acgaaacgga tagatgaact cttctgcttt
    16861 acatcggacc atgtcaccga ttttgaattt tgatttctga cgtgttttag ggttctttat
    16921 cggcatgtct agcattaaac cgccgatacc atgactacta gcgtaaaatc cgtcttttat
    16981 tttcatctca tttcctccca tttacgatca tcatttaata tcgaaatccc aaacttacga
    17041 atagcattac tcgcatcagc aacaaactga cttgccactt tatatgtttc ttctgctgaa
    17101 attccatatt ctttttcaaa ctttgtcttt agtacattca gttcctgttt tcttagtttt
    17161 gttattctgc gatgtctgtt gttcattccg cttcctcctg ttctaatccc cattgagcga
    17221 atgctgatag gacttcatat tcttgtttac aagccaatag tttataagct ttgcgtactt
    17281 tatcaggtaa ttttcctagt aaatttttat cagaaaaagc attaacagat aatattggtg
    17341 cttctctagt taaaattgtc tcgcttttca accactccaa cacgattttc tggttgtcgt
    17401 tgagttgaat tgatttttct atttgtctta aatcttccaa ttcaagtcgt agatgactga
    17461 tttgagtcat tttagcttgc tcaacaattg gaaaccccaa tcccaaacta tcttctaaat
    17521 tttggagttc gctttctttc tcagatatga gttggtgcaa ttttttcatc cttctgcctc
    17581 ctctttagct gtcaattact tactcgcaag tcactaaaat ggctacttca tcgataaagt
    17641 ttccatcttc gtcttccatt ccttgctgaa tgctaatgtc ggtaatctgc ttaaaaaatc
    17701 cgttgtcata gccttgtgcg tttatcacta cttcgacgtc tccgaaaacc tctttgagac
    17761 tattaatttt atttactact tccgatgcgt ccatgactac acccctttcc agttaaaaat
    17821 ctctgtcgtt aatagactac tgttcaaagt attcatcttc actcatcctt ctgcctcctc
    17881 tttagttgtc attagtggac taaatactac ctgtttgaaa accataatgt gcagtttcaa
    17941 cgccgtcata atcacttatt tcccactcag ccccatcggg tatatcaacg actttaagac
    18001 ttgcatatgg tccactggcc tcacttccta gtttttcgac aacagcaacg aggtccttat
    18061 cacctctgtt tgaatcactt gaaaaactgg aatcatcagc aattccgtac agtcggtcaa
    18121 tttcgctata aattcctgta actttgtcga ttttcgggtc ttttttgaaa tagtagataa
    18181 aatccatcga acctagcttt tggcctgtgt atctcgtgta ttcatcatct gagtcatagc
    18241 taaagttatt gatgtatggg aatacttcaa tattttttct tttaaaaatt tccatttttg
    18301 cttcatggct caacccaaag ccaccaaaac atttatttaa tacaatcttc attacttttc
    18361 ctcctttgtt tcctccgtta gctgaatttt ttcataaaca caagccaatg tgttttgctt
    18421 cgtttattac caaacaatgg ctgttctcca atagcctcta acacatcaga aagtggtatc
    18481 tgttcctcgt tccacttaaa aattaatgtt cctgaaggtc gtaaaactcg catacactct
    18541 gcaaaacctt gcctgatgtc ttctggccaa agttcgtcta attttccata ttttttggca
    18601 agccatgagt tttcgccgac atgtcttaag tgtggcgggt caaatacaac catatcgaac
    18661 acatcatctt caaaaggcat atcgcgaaag tctgcgacca catctggatc aacattgatc
    18721 acatgacctg ttgataattc ttcatactgc tgacgacaat ccataaatgt tacgtcagaa
    18781 ttgttcttat caaaccagaa caatctacta ccgcaacatg catctaatat ttttggcaat
    18841 tactctacct ccaataactc tggattctcg tagacgttac ctaaaacagt gagaaatctt
    18901 tctgtatccc gataaataat tagcttactt atcttccgaa ccattcgact ctcttgttga
    18961 gataattcgt ccatgtcacg atcacctata atttgcaaca ttatctgacg acagattctt
    19021 ttttcttgtt tagtcaaatt catgcgatcg ctcctttgac tggttttatg cgcttgtctg
    19081 ctgtttgaga aaataccatc gtaaatccat cagagttgac aaacattctt gatattgttc
    19141 tcgtaccgta agcttttttc agttcttcac caagcaagtt cgttgtgata atcgttgcta
    19201 ggttctgtcg tgcatccaag aacgaattta gcgtgtttat gccaaacgct cgactatctg
    19261 atgcatcttt tcctagttct gaaccgatat catcaatgat gactaaatcc gctgttttga
    19321 tatctgcaat caacgatccc tcaatcgttt ttctgagttc aggattgttg tacgaaaatt
    19381 tgatttgatc cagcatttcc tgtaacccga taaataagat ttttttatcg tagtttgatc
    19441 gcttcaagac ttcccaagcc gctgccatcg ctaggtgact tttacctgtt ccttgttttc
    19501 cagttaaaac aaggtgactt ggatttccta acaagactga attaacaaag cgtttggtta
    19561 cttcaaccgc ttgtcttgtt tcttgatcga caatttgata attctgcaat gtgcaatcaa
    19621 acaacgcttg attaggcaca acagaaccac ctttgaaaaa attgattgct cttgctttca
    19681 ggctctcgtt gtaaattcgt tctgtttgta agtcttcctg aactcgcaat gctttatacc
    19741 cacattgcat acaggttggc ttacaacgtt ctgagccatc agggttcttc gtacgccatc
    19801 catacaaagg ctgtccgcat tcaggacatt cgccacgttg gacaagcact ttctgtatca
    19861 gcttttccat gatttcccca acagtttcca tgcctacgcc tcctctcaaa taggcaactc
    19921 atctgtgcta aatttctcgt attcaagcgt tttagtttgt tttgttttat aatcacgatt
    19981 ggcttgcgct gccattgtgt cgtatttttc tcgcaatttt ttagcagata aaatattcga
    20041 tgcccaaaac acattgtgct gactccattc aatcattccc cgtacctggt tttcggtccg
    20101 cttgtcgatc tcgatcattt tccgaatgtt atctgcccaa ctttgcagat tcggcttttt
    20161 gatttcctga ttctgacaaa tctgtttgaa taactcctcc gcgagaatgt agtaaactga
    20221 gtcggtgtcg taaacacgct ttttgcgtgg ttgcgacgat gatgttttat tattcttttc
    20281 attcttataa ttcttttcat tcttgtttgt gtgcacttgt tgttcacttg ttgtgcgttt
    20341 gatgttcatt tgttgttcac tgctttggta caaagaccag ttttttattg atataacgct
    20401 gtatttcgta gttgatttga tgttcaacat tccgtttttt tcaaattgtt ttagccatct
    20461 ccatacagaa ccgctgttca cttgatgttc acgtttcacg ccttcgttca tctcagatgt
    20521 gatagcatcg cgccctgtga cgaattctcc gctgttcact tgtatctctt ttccattaaa
    20581 aagtatcttg cgattctcat gactcgcttt catcaaacac aagttccata atttatacat
    20641 ataaggattg gtccaaacga atgaattcat cacttttcga tgtaatttga catatcctgt
    20701 gttcattcgt atttctcctc tagaaaagca agggagaaag ctccctcatt atttgtttaa
    20761 cggcggatta gatgcatcaa ataatccagt ttgtacatct tcattttctt cagatataac
    20821 ctctgcttct tttctttcag gaatatcttc ttcaacttct gtttcagcaa taatgctgcc
    20881 gtcttcttga accctttgga ctctctcatc cgatgtggtg gcttcttgca tttcgatgga
    20941 caagattccc catttagaaa gaagatttct caaaacagtt tttcgtgcca ttgcattgta
    21001 atcagatgcc cacacaccac ttaactttgt cttatcgcga tctttattgt tagcaatccg
    21061 atgagcttcg atttcttgtt tggtccaata gacagttttt ttgaatccat tcagtaactc
    21121 gaaatagcca acatatccaa tgacttcatc agacgttcta ccatttggat caaactcaaa
    21181 ctcttctgtc agtcggttcc agctttttag ttctccttcg taaacttcga tcacatttaa
    21241 tgctttgtat ttacctgatc gttgggctaa ttggatatat cctttatagc caagcatgaa
    21301 ttgagctttc ttttcccatt ttcctgtttg cttgttttta ctattgaatg gaactaaata
    21361 tgcataacct aaattcttat ctagcccaag atttaatgtt gcagcagtta acgcaccact
    21421 catgatagac atcggttcac tatctgcaag ataactgtca ttagatacaa gagtcataac
    21481 attcgacata aaagcattag cattgtcatg aagcacctct tcgaacttct ttctcattgt
    21541 tggtgtattc atcaaagctt taagtcctaa ttgacctggt gcaacttgtt tctgtggctt
    21601 tgctgccaat tgatttttta acgattcatt tgttgccata ttatttgatc tccctttcgg
    21661 ttagccttct tgattcagta acgttataaa tctcttcatc atttgcgaca tctggatatt
    21721 tctctgctag tttcttcgag ttcatacgtc tcgtacggac aaatttccaa ctgatgatgt
    21781 ttttttgagt gataccgata ctggcttcac gtttacctag ctcgctgata atctcgttgt
    21841 ctacttgacg gatagctgat tcaatttctt tcttagtccg tttgagttcg tttttctgtt
    21901 caactagttc atcgaaatga attggtaacg tcgtttgaac gtcttcaaca tctgcatact
    21961 tctctttcaa gaaatcagct gtcgcttgac taccatcaat aattggctcg atacctccaa
    22021 gaacgtttgt ttcccaaaac tctactaatt gttcagtgat tgtatcgatc agctcttgat
    22081 ctcgttcaat ccgcttccag ataaattttt gaccaccgat taagacagcg atgtaacaat
    22141 agtctttgtt tagaacgttc atgtaatgtt gaacttgaca gagatagcta agcggtacct
    22201 cttcgccttc ccattcttta ccgagaaatt gattagctgt tttgcattcc agaatggcat
    22261 tttctcccac tacttcccga tcaatatttg ctcttaaaaa tggatgtaat ggatgttcaa
    22321 atacttggtt tcttctgcgt acttttttgc ctgtacgttc ctgaaactct ttagcaacca
    22381 cttcttctaa aacattaccc caataagcgg gctcacttcc tgattcttca agtactactt
    22441 gtcctgtttt ttctagccat agttgataag gagatttcca tttattcaat cctaaaattg
    22501 ttccaacatc cgaacctccg atgcctttct tacggtcttc aagccattct tgatggctca
    22561 tttctaaggt agatttactc atcttcatcc tcctcatcaa ttggtgcttc atatggtggt
    22621 ttagcataat caggatcagt taaatagttg tcaagatttg ctaactcgtt catgtttaac
    22681 ctcttttctg cttgctaaca cgcaaagcac aagcatggta attgcgaata acaagccaaa
    22741 gattacataa cttttcgcta caatcactaa cgtgaaaaat aaaataatga gtagatcaat
    22801 tgttttttca ttcatcattg aaattccccc ttgagaatac gagtcatcac atcagctaaa
    22861 tcttccttgt tattaataac aaaagtatga tttgaaggtt gattttcttc ttcatttttc
    22921 gcttgttcgt aagcatccac agcaattcta cgcataatat gtgctgacaa tgggagattt
    22981 tcatctaaat gttcttcttg catagcaaga caaagtaaaa tttctgttgt ctcaccataa
    23041 acgagacttt taagttcccc ttctttttgg gctgtacaaa taatagaaac accttgcttt
    23101 tggcattcac gtttcaattc ttcgataaga ttttcaattt tcttattcat gtgatataat
    23161 tctcctagtt ttataaattt gtatgtgact ctttgctggc tggcagagtc acttttttat
    23221 ttgttgccat gctttttgct tttcgatatg ttgcttgctt aaaataatag ggcggctatt
    23281 tccccaccga ttatcagcaa tcactttacc gatttttagc gcttcttctc gtgccatagt
    23341 tgctcctttc ttttgaatca agcagattga ttaaaaccat caatgctgcg aacaagcttc
    23401 ccccgataat actttggtgc gctacaatca ctaataaccc caaaatgaat cctataaaaa
    23461 gtgtgtctgt cttcttcata atctaatctc cctatttttt atttctagca ttctcaaatc
    23521 ctcaagttca gaagcaatta gttcagcttg cctatctgat agctcatcgg cttttctaag
    23581 cgctgcacgg tcatcttgta attgtttcct gcgttgttta atcaaaccga gaatttgatg
    23641 ttcttgttgc aaggtatatg ccatatcact tctcctttcg tttattgaat ttctccattt
    23701 ctatttgata aaatatttct atagaaatgg aggtgaaaac tatgggaaaa aatcaacatg
    23761 ttgtacctaa ttccaatggt ggttggaatg tcaaaggtgc tggaaattct aaagcgactg
    23821 cacatactaa tacaaaatca gaagcagtaa aaatcgctag agagatttct aaaaatcaag
    23881 gatctgagct atttattcat ggcagagatg gaaaaattca aagccgtgat agtcacggaa
    23941 acgatccaca cccaccaaaa ggttaatcat atttaggagt taatcgaata cgataaccgt
    24001 cagcaggagt tgcatcggtc aatgtaactt ctgctacttt tttcccttct tctgtttcaa
    24061 taattaatct tgtgtaattg ttctcattca aaatccctat ttttgattct tccttcatgt
    24121 catccctcct tcgtctctcg ccgtcaccac ctccaagaag aggagttgcc aaaatgaaaa
    24181 aacgtggact ttggcaaact tgaaaggaga gcagcaactc ctcttttcag aagtggtgaa
    24241 tttagatttt ttagctatta acaaacagct ctaactctaa atcgattttt agaacgccat
    24301 gttctaaact gttcatataa atcgacatca attgttattt ctttggaagt aactgcgtta
    24361 tacccctcac taaaacgtga ttttttaaat tccttcattc ttctttgata agtactttca
    24421 gaatacttat atttctcttt aaactccgct ttgctcaaac gttgtaccat ttttacgcct
    24481 cctgttctga tggctgatca ttagaatcta cttctaactt gatatccaaa agttcttcga
    24541 tttgttttaa acgttcctta ggttttcttt tttcgatgaa gatatctcgt aaataagtaa
    24601 ttgatatacc cattcgtcga gctaactctc tttgactcca acctttcttt actaaagcaa
    24661 ttaatacttg ctgttttaaa gtcatgcttc ataccccctt atcgaatatt ttctaatatt
    24721 tttgtctaat aagtttgaca aaaactgcaa atatgctatt atgtaagcat acaaaataca
    24781 gcgcacaaaa agcgagctta ctgatgtctg gggagactat tagtttattg cttttagtct
    24841 ttgtatgccg taaatatctt gcttacaaaa ctagtataac tgcaaattct ctatttgtaa
    24901 atagataatt tgcagaaaaa tacttttttt gtcggtattt tgaaaggaat gcatatccta
    24961 tgctattaga ccgtataaaa atcttattaa aagaacgttc aatgacgttg gcagaacttg
    25021 aaagaaagtt aaatttcgga aatggaagca tgtctagatg gaataaaagt actccatctg
    25081 gagataaaat acaaaaagtt gcagattact ttaatgtatc aactgattat cttttaggac
    25141 gtactgataa acgccattat tatgatttaa cagaaaaaga taaacaagat attgctttac
    25201 aagctgaaaa attacttgaa ggcttgaatt cagatgctga aacaaattat tatggtgaac
    25261 ctttagacga tgaagataag gaaaaattat acgatgccat tcaacttgca cttgccttaa
    25321 ccaaagttaa agcaaagaaa aaattcactc ctaaaaaata tcgtcaatag gtgggctttg
    25381 gtatgcattt aaacaaatac tggatattag aagaaataga aaaattattt gtagagtttg
    25441 gatctgatgc gattaatcca tataaaataa tcaaatattc tgatatagat ttaattgaaa
    25501 ctgatttagg gaataaaaca ttaggacaaa cgatacacaa taaaagatgt tatgtgatat
    25561 taattaatca aaacctatca ccttctatga aaagatttgt attatggcat gaaatagccc
    25621 acgttagact ccataaaggt atttgcacag cagcttttag agcaaacaac cttagttgta
    25681 tgatttatgg tatagaagct gaagctaata tgtttgcaat tgaaatgtta actagaacaa
    25741 tagatgaaca agatttatct gatatgagtc agttccaaat tattgaatat ttagggcttc
    25801 ctcataatct tgaacattta gtattacaac attaaaaata aaaaagccca tgctttcaca
    25861 tggactcttt cctcatttct gagtaaactc tacaataatt atatcaaaga aatgaggagt
    25921 tttgaatgtt attattgatt ggacttattt tattaattat aggaatcact aaactgtcga
    25981 aagctaacac aataaaaaaa gaagcttatg aattagaaca aaaaaatgat gaaaaactaa
    26041 ataaattaca tgatagagaa agatttatat taagtaagga agaaacacta tcagatttaa
    26101 ataaacaaat tactatgtta aaaaatgata ttaattgatt acgtccatat aattgtgtaa
    26161 aagtgaagca tcaaaaaagc cattgatctc agttaggagt acaatgtttt ttgcagaaaa
    26221 catactcgaa taggactgat atcaatgact caagtacatt ttacttttga aagcgaagaa
    26281 attcaagcaa ttataaacga aagcggtgcg aatgataccg caaaaacact tatgactatc
    26341 atgtttaatc agctgatgga agaacaacga aatcaatata ttcaggcaaa tgcctatgaa
    26401 cgttcagaag aacggcaatc acaaagaaat ggttactacg atcgttcgtt tacaactcga
    26461 attggtacgt tagaacttca tgttccacga accagagacg gtaagtttag tcctactatc
    26521 tttgaaagat atcagcgaag cgaaaaagct ttgatcgctg ccatgattga aatggtcatt
    26581 tctggtgtct ccactcgcaa agtaactaaa acagttgaac tgctaacgga tggtgcgact
    26641 gtttctaaat cattcgtttc aaatctgatg aaacagttgg atccatttgt ttttgagtgg
    26701 agaaaccgga gcctagaagg ctcggaatat ccatttttca tgtgtgatgc cctgtatatg
    26761 aaagtaaggg aaaatcatcg tattgtctca aaaggtgtat atattggtat cggcattgat
    26821 tctgatggac gacgtacgat tcttggtttt gatgttcaag acggcgaatc ggaagataat
    26881 tgggataccg tttttcaatc gttcgttcaa cgaggtttgt tcggtgtaaa actggtcatt
    26941 tctgatgcac acaaagggtt agttaaggct gttcgcaaga actttttagg tgcgagctgg
    27001 caaagatgcc aagcccactt tttgagaaat atttttgata agctgccaaa gaaagtttct
    27061 tctgatgtaa aagacgagtt gaagagtatc tttaaagcct ctgaacttga attgacacgt
    27121 gaaagaaaag agcacttctt ggaaaaatat ggttgtgatt caaaattaag tgctgcttgc
    27181 gacattttag aaaacggctt tgaagatgct attcaaattt tgtcttttcc tgaaaatatc
    27241 cgccgaagga ttcgtacaac caatgtgttg gaacggttga acgaagagat tcgccggaga
    27301 gaacgagtga ttcggatttt tccaaatatc aattccatca ctcggataat tggaacactt
    27361 ttgatggaaa aagacactga gtggctggct tctccacgaa aatatttaga atttaattcg
    27421 aataacattt agcaccatga acctgtgcga gtctcttctt tctgaggaga gacatcaaga
    27481 gtcgctgcgc tactgctcct gacctctctc ctcagaaaga aaggcatggc agttaggttc
    27541 acaacgcaaa acattgtgct tttgatgagg ttgaactttt acacaagatt gtggacttga
    27601 ctaatttcat tgcaaaaaaa ataataaaca cttataaata tccattatct atccttcaat
    27661 ttttgtgtat tttatttcta ttagaaaaag gtatcctgtt ggacaatttt gttatatcag
    27721 gtaaacttca ttgcaataat gataacaaat gtactataat tactttgtaa agagttttgg
    27781 ggaagactct ttcatcaacg tgtattaaga gacaagaaag aatccatatg gattctttct
    27841 tgtctctttt tccactactt tttatttttc tgacactaca tattcttata actcatttct
    27901 cataactgtg aaacaactta taaaaataac aaactttaaa caaaacaagc ttaaaatcaa
    27961 agatgatcca tcttaaaata actaaaacaa aaaaagtagt cgttgttaat cctcattaat
    28021 aatcgttttc tttttttcaa acgctccatt tttatgtgga acattatttt tttaaggaac
    28081 tttgtcaaca tgtggataac tattttccac ttgtgtcctg gactagctgt tttgctgtaa
    28141 aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg
    28201 gatctagatt tggcaaagtc aagactgcat catgtaaatt ggcgtggttg tccaaaacca
    28261 aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg cgatttgata cttcgtgaca
    28321 aatcaaatcc agtaacaatg attaatggat gaccattttc tttgatccac tgagctagtt
    28381 cgatgattgc cgggtttctt ccttcattcg aaagaagaag aaaaatgtct tctgatttga
    28441 cagaaagctg atcaataatt gcctgtcctg caccttctag cgtttcatag acacctccgg
    28501 ctggattttc aatcaatata cttggtacaa gatccggggt ttgatcccaa aattcaaatg
    28561 ctacaccact tagtgtgcgg ctagcatata tctgcaatct accaccttga ttaatttgtt
    28621 tcgcgatcca agtgccagct tgaagcatca gcggctcttc tatccttaat ttctcttcca
    28681 attggtgata atagtcaaaa taacataaac tcatttatct ctcccgccta gctatacttg
    28741 acatgaatag aaacagctgc ctaatctaag gaagtacata aaaaaacagt agttagacag
    28801 taacacatgt ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat
    28861 accttgacat agcaaataaa acaacgtgca gaccatggaa aatatctaca taggggtaga
    28921 attttcaccc tagattaggt acaataaatt aggtcgatgc actgcattga ctatggcaaa
    28981 atgatcggtc agggtgtatg tgtgtgactg gtcattccct gattcttagc gccaactaag
    29041 ggtcagggag ccttttttta ttcagctgtt tctatctgaa tttattgtaa gtaatctctt
    29101 atccaagtgc aagcaagaaa atacaaaaaa acattggtat gatttatata tatataaata
    29161 acataaaaaa aggaatggct tatcccgata caacaagcat tcctttaaaa aagattcttt
    29221 tatataagtg aaaaaagctt ttttccacca attaaataaa tctctcatct caatagttta
    29281 tccattattt tatctttttt tgcgtatacc tactaaatat taccgtcttc ttctcgttct
    29341 tctactttgt tcgagagaat ctgtactaga aaaagtagtt gtatgtctac cttttctgcc
    29401 tatccgagac tttcttttca tatatgggat ataaatgatt agatacagta cagcaaaaaa
    29461 taaagcaaat acgcaagctt cccaactacc taacgccaat tcaaccatca aggtagcaac
    29521 cccaacggat gctgcatacc agataaaaat cagaaacgtt tctttttttc tgaccatgcg
    29581 aaacatgata aatagactaa taagcaatag tggcaacaca aatatccaat tgcttccttc
    29641 catcagccac ttgagtatat caagcagcga ctgtgcaaca gcgactaatt ttcccatagt
    29701 gaacacacac ctttttttat aattaaaaaa ttcgcctttt tattctacta tgaatcttct
    29761 ggcttggctt tcatttctct tatgtaatca ttttctttta tacaacttaa aaaaaaatta
    29821 aaccattagc ataatatttt ctgaatctat tcacgaaagt ctttttctat ctttcgtttt
    29881 ccacgactca actgatctag agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca
    29941 agctgccgct ttaataacaa atagcctttc attctcagca ggccacttat ttccagttaa
    30001 ctttctgttc ttagcttttt actcaactgg ataatatatg cacttaatga agtcccaatt
    30061 tccgaatgtt gaagtccata ttcaatattt gtcttcatat atcctaactt atcgcctacg
    30121 tcgtatcttt ttcctttaaa ctctctcgca tagaccgcct ctgtctggtt aagagtctga
    30181 agtgcatccg ttagctgtat ctcgtttcct gctcctggtt cttgagtttc taatatctca
    30241 aatattttag gagtcagtag ataacgtccg ataatggcta aattacttgg tgcttttccc
    30301 atttcaggtt tttctacaaa cccttttact ctgcaacgac ccttttcgct atctgtttta
    30361 ggatcaataa ttccgtattt agatgtttca ttttctggta cacgcatgac ggcaacagcg
    30421 gaagcttgtg tttcttcata atccatcatc aattgttgag tcaatgggat atcatctttc
    30481 atcaaatcat cccccagcat gacgacaaac ggttcatttc ctataaatgc ttttgcttga
    30541 agcacggcat cccccagtcc tcttggatga gattgacgga tgaagtgaag attgacttga
    30601 gtcgtttctt caactaaagc gagtaattct gttttttcct tttcccgcaa attgttttcc
    30661 aattcgatat ttgcatcaaa gtgatcttca atgggtcttt tttcttttcc cgttacgatc
    30721 aagatgtctt cgattcccga atccaatgct tcttccacaa taaactggat cgttggttta
    30781 tccacgattg gaagcatttc ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct
    30841 agcccagcgg caggaatgac tgcttttttg accttcactt gctgttcctc ctaaacgacg
    30901 attaactatt gatcatctat ttaacgaaga gaaaattcat tctccatttt tccgtcacgt
    30961 aacatgattt cttttgctgc ttttttgata tcttctccat tatacaaaac attatagatt
    31021 gtttgagtaa ttggcatctc aacacctagc tgttgagcta gctctactgc tgcttttgtc
    31081 gttgccacac cctcaacgac cattcccatg ttttctagaa cttcttccag cttatgtccc
    31141 tgtcctaaca agttaccggc acgccaattg cgagaatgaa cacttgtaca agtaacgatc
    31201 aagtccccca caccactcaa accgataaat gtcagaggat tggcacccat cgcgacacca
    31261 agacggctga tttctgccaa tcctcgagtc atgattgctg cttttgcatc atctccgaat
    31321 cctaatccat ggatagcgcc ggcaccaatt gcaatgatat ttttcagtgc tgcaccagtt
    31381 tctacgccaa tcacatcatt atttgtataa atcctgaaat aatcattcat gaacagtttt
    31441 tgtacatagg ctgctgcttt ttgatcaata cttgcagctg taattgttgt gatatcatgt
    31501 actgccactt cctcagcatg gctaggacca gataatacaa caattgcctg tcttttttct
    31561 tctggaattt cttcagccaa aacttctgag attcgtttat gcgtatcttg ttctagtcct
    31621 ttacttgcat gtataatgat tggcttagta ttcattttac caataagttc ctgtgcgact
    31681 gaacgaatcg cttttgttgg aacaacaaat aaaatagcat ctgcgttatc cactgcttct
    31741 gccaattctt tatatccgat aatcgattct gggatcgtca aatcaggtaa aaaatgctga
    31801 tttgtatgat acttattgat ttcatcaatc tgttcaggta catttcccca aatacggaca
    31861 tcatgcccgt tttctgccaa tgtttgtgct aaagcagtgc cccaagatcc aggaccaaga
    31921 actgcgattt tttgtttcat ggatattcct ccaaataaat aattgataat ttccattata
    31981 gtatacattt cttaaagttg ggactaattt ccattgtttt taagtgtttg tttacttctt
    32041 ttatcgtttt tacttgaaat aatcaatgat atgttacaat agtctacatt ttatttttac
    32101 actcgttatg ctttgcttgt tcctaagagg aagcagtttt gacaatgtta ctaccagcat
    32161 tactattagg aggttaggaa acttctccta gtgagtattg atttaaaata cataagagac
    32221 agattgaaca tttctaaatc tgtcaggtcc tgcaacggac agattgtctc tgttgacggt
    32281 tgacatagga acttttgttt tagtcaacat tctggataca aaacgctatg taaactggcg
    32341 caactcgttg tacttggact tttactacgg atccatgcaa caactaaata gtactattgc
    32401 ataacaaaaa atggttggca cccacatgtc ccattaaaca gattataagt tgaactgtag
    32461 ataatgcaaa aaatgtgttt tataacgtaa ttgatgccaa tattttaaaa aaataaaggg
    32521 agcagttatc gctgctctct ttatttttta tattcttttt atccagtcta tcctaataat
    32581 caaactgacg atagtagcgg cgtatctcta atggatggca gttcaactgc tccacatgag
    32641 catcgatgcg ttgtgtcact acatgcatca ataagaaaga caaaacacgt ccgcgatatt
    32701 tttcactgat accttctact ggatagtctt ttgtgtctat caatgtataa ttgctgcaaa
    32761 tttttggcaa aagtttagcc acacgttcag ataacactct ctgttcatct tcgcctaaaa
    32821 ataatgtgac tggggttgtt tcttctacga tctctaacgt cccgtgaaga aactcggcag
    32881 catggataga tttcgatgga agccagcttt gttcttccca gtaacacatg gcatatgaat
    32941 aaacagctcc ccattgattc cctgctccaa taaaataatg catactatca cgtcgatgtt
    33001 tttctgcaaa tgataagcca aatgcatctg cttttttctc tgcttccact aaccctgttg
    33061 gtaaataacg ttcgagctgt tcatagtatt ctgaataagc ttcgaattct ccattcaaat
    33121 acatcaatcg atctgctgcc ataaaaaatt tgatttgttc tgttccagga gctggataag
    33181 tgaccacaaa atcgcatagc tgagacaact tactgttcgc tttatcgata aatccaatca
    33241 gtgtcgctcc tactttcttg atttgttcta ctgctttcac tacttcttga gttgtaccgg
    33301 taacagagga caaaataacg atagaatctt ttgttaatct tttgtttcca gtagtgtaat
    33361 attctgcagc atgctgtaca aagacaggca agttgctttt cccattcata taagtgacag
    33421 cttgcatcga ggaagcatac gttccaccga ttcctagata atagacagcg tcaaattttt
    33481 ccgcataaag tttatcgatg atttcttcta cttgcggacg taaagctagt gcgcctttta
    33541 ttgctttgat ttgttcttct tcattgaatt tcagcatgat ttccacctct tataatttac
    33601 ttttcaagac tgtatcaaag tcttcaaaaa tctttttgtt caagtcaata cctttagcta
    33661 ctgcggtttt ataagcgaat agttgaaacg gaacgacaag cgctaataca gacagatatt
    33721 catcacattc tattgctaat ccaagtgttt tctcgtttct tgcttttttt gtggtaatgg
    33781 tgagagtctc tccaacgtac cgactcatat atctttgcaa cttttgcgaa cgttccttat
    33841 ttacactttc cccttcaata aagaatagca aatgcgaagc atctgcctcc aggtatgggc
    33901 cgtgcatata agcttctaac tcaaatcctt gtgacggaac gcggattgtt tcagtcaatt
    33961 tcgtctctgc ttctttagct gttccccagt ttggaccata tccaatggtg acgaaacgct
    34021 gtgctaaacg aaataaactt tgatatccat caaaaaattc ttccgttttt tgaataatcg
    34081 ctggtaaatg gttgattatt ttgcgcaact gttgcatgta gtcttgttct atttttttgc
    34141 tgattttatt tttacttatt ccaattccta tccctaagag tagtaactgt aaaacagtcg
    34201 cagaatagcc ttttgtaacg aagcctactg attctacccc ggcacctaaa tcaataattt
    34261 gtgcagcttc taaagcaagt ggactttgca cgtcatttgt gatagcaaca acaggtaacc
    34321 cttgtttttg tatcattttt actgcttcaa cagtagaagc acttttcccg ctttgtgaca
    34381 cagcaatcac agtatcgatt gaaggagaaa gttttccata attgaaaaaa taaaaaggtt
    34441 cttgaatatc gacagtaaga tcaccataag attccaatgc tggcttcgct gctaaacaag
    34501 cgttataaga tgaacccgtt gctaagatca gtaaattcac tgtttttttc atatgttctc
    34561 ttgtcgaaaa atcattcttt ttcaagatcg tacttagtac ttccttttct tcatagatat
    34621 aatcttgcat cgtaaacatt ttctctgctc cttttttaaa aaatccctat ccacgaacca
    34681 aaaattccaa agaaagctag tccgatcaag atcgttaatg gtttcacttc tttcttcaat
    34741 agataaaaca ctatgccaaa tgctccaaga cataagatat ttggaacgat atcatcaaaa
    34801 atactttgaa cagtcacagc attttctcct gaaccgattt ttaaaggaat attgatggtg
    34861 atcatcgtgg cagtcattgc tccaacgacc atcagtccaa taatagacgc gcctaatgtc
    34921 aaactttcca tcataccgtt tgcctggatc tttttcaaaa aacccgttcc taagcgatat
    34981 ccccaacctg ttgctagata gcgaaacaga atgtgcggaa tattgaaaat caaaatgaac
    35041 aagatagggc ctaaaatatt tccttgcatg gctaaagagg tacccacacc tgtggcaatc
    35101 aatcgcaaag ttccccaaaa gaaagaatct cctaaccctg caagaggtcc catcaaagat
    35161 gttttgatac tatcgatcgc agtcacatca aattcaggat catccttatt ttcttcctcc
    35221 attgcagccg tgattcctaa aatcagtgta acgatatgtg gggtcgtgtt aaaaaactct
    35281 agatgccgtt tcaaagctgc tgccatttct tctttcgact gatataactt tttcaaaatc
    35341 ggaatcattg cataaacata agccatattc atctgacgtt cataattcca agaaaactcc
    35401 atttgaaaag aacgccaaaa tacgtgattt agctcttttt tcgttatttt gctgtttttg
    35461 atctcagaat tcttcatctt cttctacctc ccctgcagtt gtttgaagtg gtagttgacg
    35521 gacattcata atattgacta ccacaactgc taaaatcgca ccaaaaatag ctactccggt
    35581 cacaggaatc tctaagtaag aagctatagc aaaacctaag aaaaaatatg gtgcaacttg
    35641 tttgttgatc agcaaacgag caagcatcgc aaaaccaaga gctggaatca accctgtagc
    35701 tactgacaac cccttttgaa cgaagtcagg aatcatattt aatagatttc cgatcgtatt
    35761 gcttcccacc atataagaaa ccattaccac aacaccaagc atcaaggaaa gaccgatacc
    35821 ggcaagcaag tgcatatgct cgactccttt ataatttcct tcttctgcat aattgtctgc
    35881 tttatggttc ataattggaa tcaaaagccc taagtaaata tttttcaata tcaatgtcaa
    35941 cgtagcaatc ggcaaaccta gtaaaagcgc ggtttctgta ccggctccag ctgtaatagc
    36001 aaaagccgtc cctaaaatcc caccggtcac aacatcaggt ggaatcgaag cacctactga
    36061 gaacgatccg ataaatgcta actctaaagt tgcgcccata atgatcccag tttttacatc
    36121 acctaacacg attcctgtaa acagccccgt cacaatcgga cgagaaagca acgatgtccc
    36181 tagtgcatat tctgattgtg cgacaaatgc aacaattcct aataagactg cttgtacaat
    36241 cattgaggtt cttcctttct tacaaaactt ctcttacatc cactttttta tctgctggta
    36301 cttgacggat ttctacttct atttgtttgt caattaatcg tttcaagcgt tcctcctctt
    36361 ctggtaaaat atttaccgct tttccgatgc tgcgagtacc aggttttgcc ttgatccctc
    36421 ctaagttgat ggttttgatt tctggatacg cttctgccag cttatatgca tcctcgatcg
    36481 attccactac gatgaataac ttatatttat ctgtcacccc gcttttcaac gcggcaatcg
    36541 agtcatcaat tgatttgatc actagtttga ctccttgtgg cttggctaat ttgatcgttg
    36601 tctttcgtat ctcattcgta ggtacatcat cattagcaat caaaatacag tctgcgccta
    36661 ttgtttgtgt ccatgaaaaa gctacttgtc cgtgtaataa ccgatggtcg actcttgtca
    36721 atacaatcat ttttcttcct cctaaaattc gtcttcttct attttctgat tgagccgctc
    36781 gttacaaaac tggatcattt tttttgaatc agttagcacc tgctggatca aaacagttgt
    36841 ctctacttca gcgtccaacc gagatactag ctcaatcaat agtggcaaat tcaatccggc
    36901 aacgagatag acatgatccc gattgatata ttgtaagaat tcgttgttga cacttccgcc
    36961 aaaaagatcc gtcagaacaa tgatttcttt atctttgtac ctcataaaaa gctgccttac
    37021 tgtttccgct aaatcaaaat tctcatcagt atagcaatcg aatacagtaa tagaatgatt
    37081 tttcccatag atcagctcta atgaatctaa tatccctgaa gcaaattttc catgggaagc
    37141 taagataatt tctctatcca ctttttcatc tcctcttttc acttacacat actaaaaaag
    37201 caagttgcat gccaaaatta gtggcaacaa cttgcttatg ttcaaaattc ttcttctatc
    37261 gagagaaact ctgtatcttc actcaaaata tcatagatat aggcaatctc agaattgggg
    37321 atcatgacac tataatgctc ttcaatgaca ctaaacgcag atttaatagc agataaccct
    37381 tctttttgac actgcttcca tttctctaat ccttgatagg tttcgattgg catttgtcgg
    37441 atcaatcgtt caatgagaca gctcacatgg acatacaacg ctaatttttt gggattgctg
    37501 atgcttcgat tcaactgcac ctctaattct ctcataaaat gatcgatttc tcccatcacc
    37561 ttctctgtat ctagaatagt cacggaatcc agtacttttt ccaatgaaaa attacgaatg
    37621 atgcgctgat tgaatatttc cttctctgaa gcattcatag tagtgtccaa ccattcagct
    37681 agtacagaca tcttttcccc cgagatgagg tcttctaatg aaagatagtc gatattggtg
    37741 atatcaggat ctgtggtgcc aatcattcct acgacttcgt atatggaaaa taatgggtca
    37801 ctgtttttct tatcttttaa ttgctgatat tcatatggga tgattttcaa ctgacaagcc
    37861 gtaggtaagc ttttttctag cagttcactt aagtggaccg ccgtaccgat tccagtgaag
    37921 caagtagtaa tgagtgcttt gggtttgttt tcctctggat agagcagtcg ccattctggc
    37981 tggatctgtc gaacagtctc ttctgctatc tcttctaatg atacattttt ttgcaggcat
    38041 tcccctactg tgattgctaa aggtgtcgtc acattattca aaatcaaaag cggtacggat
    38101 aactgttttt taaaaaactg atggatttct ttcagagatc ccatgtctac taatatgatc
    38161 aatccatttg aaacatcgct gttttcgcaa taatcaagga tttctgaagc aatctgctgt
    38221 ggtgtgacat ctagtggcat atcaaatgat tcaaatagat cctcttgtaa taaacgattt
    38281 actacatttg ccatactgct agcagttgca taaccgtgag cagcaatcat cgcttttggc
    38341 agtcgttttt cttttgacca tttcgctttt tttagataga tcgtcagtaa aatccgatcc
    38401 atacttgtgt cttcaatatc taatttgatc ttacttaatt ccagaattcg ttggacatag
    38461 tgataactag atggataaga ctgagaaact tgttgttcta attctttgat tagttggata
    38521 atatcatgtt cttccggtaa ccatttgata gtgcttcgct gatagagata gtaactcaag
    38581 gcatagacac tgtttccatc gaatttgatt tgataggcag tttccatttg tcgaaaggtc
    38641 tctcggatag attgtgtcca ataaaccagc agctcgtgtt tttcttgtct actggtctca
    38701 aacagcaaaa aatcaaataa ttgaatcact tcttgcttta attttccttc gcaagaatat
    38761 agcagacagt tatttttctt gaaatcaatt aggatttgct caaaactttt aacgattcgc
    38821 tgctgttcgg gctgttgctt ctccattaat tgctctaagg tctggtcttg cgtaatatag
    38881 attccttgct gtgctgtatt tttagtaata ggctgcgcaa gtagttcttt ttgcaaatga
    38941 tggatcgtga catggatttc aggctgttca cgctgttcag catatgcttt tgctgctgaa
    39001 actttgacga tatttttcag ctctcctatg tttccggata atttctgact tgttaaaagt
    39061 gacaaagctg gtccactgac tgacaaaggg ctccccattt ttctttgttc ttcgagaaaa
    39121 aaagagtaaa tcagttctag ccgttcatta cgactacgct gattaagtgc aggtagttcg
    39181 attcggatag gtattcttcg aatgaaagtt gttaagaaat gactagtaat ctcttcagtc
    39241 gtagcaaaaa agagacgtgt tttgatccgg cgtgacaaat taggttctcc catacgataa
    39301 atcacacctt gatcaagata ggtgaacagt ttttcctgac cttctggtgt taagcgatgt
    39361 acctcgtcta aaaacaagat cccaccatct gctgcttcaa aagccccttt tttattttct
    39421 tctgcaccag tgtacgctcc cttaacatgt ccaaacaaat tgctagtcaa aagttcagga
    39481 ttatctgcat attgtgcaca attaaaagtg atgaatggtg cagaatcttc tagcagatca
    39541 tgaagcagac aatactgata gaccaaatta actaaataac ttttacctgt gccactttcg
    39601 cctgtaatca aaagtggtaa tccgccatct ggatagtaaa gagctgtttt gatttgttcg
    39661 atactttctt tcaaactggc gtcgtatccg atcataaaag agaataagtc ttttggttgc
    39721 gcgaaaaaag gctgttcctc tttgagatca tcgatagaaa aataaaaatt cgtgcgtaac
    39781 ttaaaaaatt gtttttcaaa agcttctttg tggaaataaa tcactggtcg agaattgatt
    39841 ttgactagta gttgctcatc atttaactgg ttcaaataat gactgactgt gtttcttttt
    39901 accccaaaat gatcagctaa cctttgtgct gtaaagacct cgttcatatg ttctaaatcg
    39961 aaaaaggctg tctggttctc taagtaacat aaaatttcct tcttcaacaa cttattcacc
    40021 cctcctccat catagaaaac ggattcattt tgtcaataaa atctttaata aagaattgaa
    40081 aaatatatct tattatagac agcagcctac ttatccctta taatttttat tctaaaaaac
    40141 gtacagttcc ttccagtttt tatactggaa tttcaacata tgaaagaata aggaggctat
    40201 gactattcaa tgaaatcgga taaatataaa aacaggaaac atacttcctg gaaaactttt
    40261 cgagaaatca tcggatctta tctcgctgta gttgtatttt tgcctttgct ttatatggct
    40321 atgacagaac aaactcgttt tattggcgta gatacgcaat atatagccga aacaggattt
    40381 ttattcatct ttatcttgat ttttgcgttc tataaatcct tccatacaaa ataagattca
    40441 cagatttcaa ctttcctgtc tgtttttagc tattacaaat ccttatatgt ggtataacaa
    40501 caatttattg gcataatgag gaaaaacgag ttatgatgct ttcagagagt aaagaggtac
    40561 tgttgtcacc atccttttta ttgattacgt acaccactct tagttttttc ccattgatta
    40621 cgaaaacaag gtagaaacat tgatgttcct accttgtttt ctttttttat tttcagatca
    40681 aaatctcaag tggcttttga cttgatgtcg ggaggttgtc ttcgttccat caaacctgat
    40741 agaatctgcg ccaaaccata gttttctcca ctattattta ttcctctaag atagtaccca
    40801 caataaacga ataaatattt ttctcatttt catactcctt ctttgaaaat caattagatt
    40861 tctctcaacc cactttttta caaaaattac gtagcaaaaa agattgcaag aacaaattaa
    40921 aaaagaagta aatagagtt
//
LOCUS       DAYYFD010000013        40017 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000013 DAYYFD010000000
VERSION     DAYYFD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.013"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..998
                     /locus_tag="ACHA25_000647"
     CDS             129..998
                     /locus_tag="ACHA25_000647"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8761974.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1163..2434
                     /locus_tag="ACHA25_000648"
     CDS             1163..2434
                     /locus_tag="ACHA25_000648"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8761975.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2431..3726
                     /gene="rho"
                     /locus_tag="ACHA25_000649"
     CDS             2431..3726
                     /gene="rho"
                     /locus_tag="ACHA25_000649"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8761976.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3825..4088
                     /locus_tag="ACHA25_000650"
     CDS             3825..4088
                     /locus_tag="ACHA25_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8761977.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4533..4982
                     /locus_tag="ACHA25_000651"
     CDS             4533..4982
                     /locus_tag="ACHA25_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8761978.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            5039..6457
                     /locus_tag="ACHA25_000652"
     CDS             5039..6457
                     /locus_tag="ACHA25_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8761979.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6457..7317
                     /locus_tag="ACHA25_000653"
     CDS             6457..7317
                     /locus_tag="ACHA25_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8761980.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7491..8264
                     /locus_tag="ACHA25_000654"
     CDS             7491..8264
                     /locus_tag="ACHA25_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8761981.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8411..8776
                     /locus_tag="ACHA25_000655"
     CDS             8411..8776
                     /locus_tag="ACHA25_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8761982.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8924..10132)
                     /locus_tag="ACHA25_000656"
     CDS             complement(8924..10132)
                     /locus_tag="ACHA25_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8761983.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10151..10429)
                     /locus_tag="ACHA25_000657"
     CDS             complement(10151..10429)
                     /locus_tag="ACHA25_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8761984.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10622..11278
                     /locus_tag="ACHA25_000658"
     CDS             10622..11278
                     /locus_tag="ACHA25_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8761985.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11720..12517)
                     /locus_tag="ACHA25_000659"
     CDS             complement(11720..12517)
                     /locus_tag="ACHA25_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8761986.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12514..13698)
                     /locus_tag="ACHA25_000660"
     CDS             complement(12514..13698)
                     /locus_tag="ACHA25_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8761987.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13723..14970)
                     /locus_tag="ACHA25_000661"
     CDS             complement(13723..14970)
                     /locus_tag="ACHA25_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8761988.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15185..16930
                     /gene="dnaX"
                     /locus_tag="ACHA25_000662"
     CDS             15185..16930
                     /gene="dnaX"
                     /locus_tag="ACHA25_000662"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8761989.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16950..17264
                     /locus_tag="ACHA25_000663"
     CDS             16950..17264
                     /locus_tag="ACHA25_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8761990.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17289..17885
                     /gene="recR"
                     /locus_tag="ACHA25_000664"
     CDS             17289..17885
                     /gene="recR"
                     /locus_tag="ACHA25_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8761991.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17949..18593
                     /gene="tmk"
                     /locus_tag="ACHA25_000665"
     CDS             17949..18593
                     /gene="tmk"
                     /locus_tag="ACHA25_000665"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8761992.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18608..18937
                     /locus_tag="ACHA25_000666"
     CDS             18608..18937
                     /locus_tag="ACHA25_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8761993.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18951..19889
                     /gene="holB"
                     /locus_tag="ACHA25_000667"
     CDS             18951..19889
                     /gene="holB"
                     /locus_tag="ACHA25_000667"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8761994.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19925..20749
                     /locus_tag="ACHA25_000668"
     CDS             19925..20749
                     /locus_tag="ACHA25_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8761995.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20742..21089
                     /locus_tag="ACHA25_000669"
     CDS             20742..21089
                     /locus_tag="ACHA25_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8761996.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21158..22030
                     /gene="rsmI"
                     /locus_tag="ACHA25_000670"
     CDS             21158..22030
                     /gene="rsmI"
                     /locus_tag="ACHA25_000670"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8761997.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22139..23260
                     /gene="dinB"
                     /locus_tag="ACHA25_000671"
     CDS             22139..23260
                     /gene="dinB"
                     /locus_tag="ACHA25_000671"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8761998.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23314..23916)
                     /gene="nrdG"
                     /locus_tag="ACHA25_000672"
     CDS             complement(23314..23916)
                     /gene="nrdG"
                     /locus_tag="ACHA25_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8761999.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24231..26420)
                     /gene="nrdD"
                     /locus_tag="ACHA25_000673"
     CDS             complement(24231..26420)
                     /gene="nrdD"
                     /locus_tag="ACHA25_000673"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8762000.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26746..27513
                     /locus_tag="ACHA25_000674"
     CDS             26746..27513
                     /locus_tag="ACHA25_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762001.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27503..29545
                     /locus_tag="ACHA25_000675"
     CDS             27503..29545
                     /locus_tag="ACHA25_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762002.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            29826..29975
                     /locus_tag="ACHA25_000676"
     CDS             29826..29975
                     /locus_tag="ACHA25_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8762003.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            29986..31500
                     /gene="dltA"
                     /locus_tag="ACHA25_000677"
     CDS             29986..31500
                     /gene="dltA"
                     /locus_tag="ACHA25_000677"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8762004.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            31497..32702
                     /gene="dltB"
                     /locus_tag="ACHA25_000678"
     CDS             31497..32702
                     /gene="dltB"
                     /locus_tag="ACHA25_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8762005.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            32747..32980
                     /gene="dltC"
                     /locus_tag="ACHA25_000679"
     CDS             32747..32980
                     /gene="dltC"
                     /locus_tag="ACHA25_000679"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8762006.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            32983..34251
                     /gene="dltD"
                     /locus_tag="ACHA25_000680"
     CDS             32983..34251
                     /gene="dltD"
                     /locus_tag="ACHA25_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8762007.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            34392..35474
                     /locus_tag="ACHA25_000681"
     CDS             34392..35474
                     /locus_tag="ACHA25_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8762008.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            35474..36277
                     /locus_tag="ACHA25_000682"
     CDS             35474..36277
                     /locus_tag="ACHA25_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8762009.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            36347..37087
                     /locus_tag="ACHA25_000683"
     CDS             36347..37087
                     /locus_tag="ACHA25_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8762010.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            37098..38102
                     /locus_tag="ACHA25_000684"
     CDS             37098..38102
                     /locus_tag="ACHA25_000684"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8762011.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(38147..39991)
                     /locus_tag="ACHA25_000685"
     CDS             complement(38147..39991)
                     /locus_tag="ACHA25_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762012.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        12843 a         7035 c         8291 g        11848 t
ORIGIN      
        1 aacttcttga aaattttcat tttcattgat tttagtagaa aactaccgaa atgtttggta
       61 aaatagggtc gttggttaag aattgtctta acaaatttag ttttacaaaa cttaggagga
      121 attttattat gccagtagta tcaggagctg aatttttaaa agctgctcgt aaaggcggtt
      181 acgctgtcgg cggattcaac acaaacaact tggaatggac tcaagcaatc cttgaagcgg
      241 ctgaagcaaa acaagcacca gtacttatcc aaacatcaat gggtgcagct aaatacatgg
      301 gtggttacaa aatctgtaaa gatttaatca ctgatttagt tgaagcaatg aacatcactg
      361 ttccagttgc tatccactta gaccatggtg attacgaagc tgctttggaa tgtatcgaat
      421 taggctatac ttcagtaatg ttcgatggtt cacatctacc atttgaagaa aacttgaaat
      481 tagctaaaga cgttgttgaa aaagcacatg caaaaggtat ctcagttgaa tgtgaagttg
      541 gttcaatcgg cggagaagaa gacggaatca tcggtaacgg cgaattagct gatattgaag
      601 aatgcaaaca aatggttgct acaggtatcg actacttagc ttgcggtatc ggtaacatcc
      661 atggtcaata tccagaaaac tggaaaggat tagcttttga ccacttacaa gcaattgctg
      721 atgctgtagg tccagacatg cctttagtat tacatggcgg atcaggtatt cctcaagatc
      781 aaatcgaaaa agctatctca atgggtatct caaaagtaaa tgttaacact gaattccaat
      841 tatcatttgc tgcagctact cgtaaatata tcgaagctgg taaagataaa gaaggtaaag
      901 gattcgaccc tcgtaaatta ttagctccag gtaaagcagc aatcgttgaa gatgctaaat
      961 ctcatatcga ctggttcggt tcagctaaca aagcgtaagc atccacataa cagatacgat
     1021 atataggaat ggagaacgac ttttgaagtc gctctccatt cttttttact taaaacggca
     1081 gaccatgctc gtttcttttt tttatctatg ataaaataca agcatatcca tgaaaaaaga
     1141 ttaataaaca aggtggaaac agatgaaaaa aatcgtaatc aaaggaaacc gacctttagc
     1201 tggtgaagta acgatcagcg gtgcaaaaaa tagtgcagtc gcattgattc cagctgcaat
     1261 cttagctgat tccccagtga tcttggaagg tgtgccggac atacaagatg ttcattcatt
     1321 aattgaaatt ttagagatca tgggcgcaac tgtccatttt tccaacaaca tactagaaat
     1381 cgatcctcga gaagtcgtat cgattccgat gccgcacgga aaaatcaata gtttgcgtgc
     1441 ttcttattat tttatgggaa cactgcttgg taaatttggc gaagcggtag taggcctgcc
     1501 aggtggctgt tatttaggcc ctagaccgat tgacctacac gtcaaaggat ttgaagctct
     1561 aggtgctgaa gtgacaaatg aacatggtgc catgtattta cggacagaaa ataaaggatt
     1621 gcgtggcaat cggattttca tggacgtggt ttcagtcgga gcaacgatca atgtgatgtt
     1681 agctgctgta aaagcaaaag gacagacagt aattgaaaac gctgcacgag aaccggaaat
     1741 catagatgtg gcaactttac tgaataatat gggggcaaaa gttcgaggag cgggtacaga
     1801 tgtgatccgt atcgaaggag tcgaaacatt acatggctgt cggcatttca tgattcctga
     1861 ccgcatcgaa gcaggaacct atctagcatt agcagctgca gtaggaaatg gtatcaaagt
     1921 gaaaaatgtg atctttgaac atttggaaag ctttatagca aaattgcagg aaattggtgt
     1981 ccatatgaaa atcagcgaag atgaaatcga agtttacccc tcgaaagaat taaaacccgc
     2041 aaacatcatg acctatcctt atccaggatt tgccacagat ctgcagcagc cacttactgc
     2101 gttactattg atgactacag gcacttcaga aatcatcgat acgatctatg cgaaacgtgt
     2161 gaaccatgtg ccggaactag cacgaatggg cgctgatatt accgtggaag gaaatatgat
     2221 catcgtgaat ggtccaaata aacttcacgg cacagaagtc gtggcttctg atcttcgggc
     2281 aggtgcttgt ttagtgattg ctggactatt ggcagaaggg acaacgacga tttacaatgt
     2341 agattatatc ttacgcggtt atgatcatat tatcgagaaa ctaacagcct taggggcatc
     2401 aattgaaatg atagaggaag tgggagctga atgagcgatt atttgacaat ggcagaacta
     2461 gagaacaaaa cgttaaaaga agtttatagt tatgccaaag aatttaaaat tccttattac
     2521 agtaagatga ataaaaagga attatctcta gctgtgattc gtgcacaagc agagaaacaa
     2581 ggattttatt atatggaagg aatattggat atcgttggtc aagatggtta cggtttttta
     2641 cggccaatca attatggacc aagtgcagaa gatatctata tttctgcctc acaaatcaga
     2701 cgattcagtt tgagaaatgg ggacaaagtg gcaggaaaag cccgtccacc taaagaatcg
     2761 gaacgttatt atggtttgat gcacgtggaa agcgttaatg gaaaagatcc ggaagaagca
     2821 aaagaacgtc cgcattttcc agccttaaca cctctttatc cgcaaagaca gcttcaactg
     2881 gaaacaacac cgcaaagact atctacccga atgatcgata tcttttctcc aatcggtttt
     2941 ggtcagcgtg gattgatcgt cgcaccgcca aaagcaggga aaacttctgt attaaaagaa
     3001 atcgcaaatg gtatcaccga aaactatccg gaagtagaat taatcgttct actgattgat
     3061 gaacgaccgg aagaagtaac ggatctagaa cgaagcgtga aaggcgacgt ggtatcttct
     3121 acatttgatc tgcagccgca aaaccatact cgagtttctg aattagtatt ggaacgcgca
     3181 atgcgtttag tcgaagataa acgagatgtc gttatcttga tggatagtat tactagactt
     3241 gctcgggcct ataacttagt cgttccacca agcggacgta cattgagtgg cgggattgac
     3301 ccagccgcat tgtataaacc taagaaattt ttcggggcag caagaaacat cgaagaaggt
     3361 ggcagtttga cgattttagc gaccgcttta gtcgatacag gaagccgtat ggatgacgtg
     3421 atctatgaag aattcaaagg aactggaaat ctagaattac aactttcacg tgaattatct
     3481 gaacgtcgga tcttcccagc catcgatatc aaaaaatcag gtacccgaaa agaagaatta
     3541 ttgatgccaa gtgaagaatt agaagaaatt tggaaacttc gaaaaaatat gactggagat
     3601 tcattagaat atacggaaca actgatccgc tttttgcgta aaacaaaaaa caatcagcaa
     3661 tttttcaaag aatttcaaga tgtctccttt ggtaaaaaaa atccgaccag aaatctgaaa
     3721 agataattgc aaccttgact gaaacatgtt aagatgttaa tgttgtaaat cgacaaatca
     3781 actctggttc agcaaatgga gcagggcaaa ggagcgaaaa aatcatgaaa gaaaacatcc
     3841 atccagatta ccacccagta gtattcatgg attcaacaac tggtttcaaa ttcttgtcag
     3901 gttcaacaaa aacttcacaa gaaacagttg aatgggaaga cggtaataca tacccagtca
     3961 tccgtgtcga agtaacttct gactcacatc cattctacac tggacgtcaa aaattcacac
     4021 aagcagacgg acgtgtggac cgtttcaaca aaaaatacgg tctcaaagac gcaaacgctg
     4081 cggaataata aaaacgctga taactcaacg tttagcagac tatctgatag ggatagtctg
     4141 tttttttatg ttttttgcca atttaaaact tcgtttgatg ctgaaaaagt cgtaggatca
     4201 taatgcacaa aacaatgaaa aactagtata tagttttaat tatccacttg ataaaaacac
     4261 cttatattaa cgaaatgaag aggggttatt tcgaagtaaa aaaccattta cctaaaatta
     4321 gaagctaagc tttcctttca aggaaggctt tttgttttgt tatagacttg cgattgtttg
     4381 tgagctagat cgttcatttt aatgacttat acacgcggat gatgggtctt tttcctctgt
     4441 tcattcataa tattgaacgg tacagctagc gatgctatac tgacctcagt tccaagaaaa
     4501 cgttaacatt ttttgtgggg gagaggtttt ttatgaggtt tgatcaattg atcgcagaag
     4561 aactgattgt tttcgatcca tcgatagatt caaaaggatc attatttgag cgaatagggg
     4621 atgtgttgaa aaaaacaaat aaggtaagaa atgttcaaaa agtcgttaag gcactattca
     4681 aacgagagga ggagatctcc acaggaatag aagatggatt tgggattccc catgcaaaaa
     4741 gcaaatatgt gacaaaacca gcactgtgtt tttttcatac agccaaatta acggactata
     4801 gcgggataga tgaaatgcca attgaatata tttttgccat cgtagttcct gaaaaatcag
     4861 cagatatgca tctagagatt ctcagcacac tctccagacg attgatgaat gcagatttta
     4921 gaaagggaat aaaagaagcc actacgccag cagatatcat cagacagatt agtcaagaat
     4981 gaaaatagta gagtctttgt ccgagtatcg atagtgaaga aaaaggagga aaataatcat
     5041 gaaaatcgtt ggtgtaactg cttgcccgac tggaattgct catacttata tgtcggcaga
     5101 aaaattagaa gtaacagcaa gaaatctaaa ccatcaagca aaattcgaaa cacaaggtgt
     5161 aaaaacagaa aatcagttat ctgaacaaga aatcaaagag gcagatgcaa tcattcttgc
     5221 agtagataaa gaaatcgaat tggaccgatt tgccgggaaa aaagtaaagc gtgtctctac
     5281 aagccgagca atcaaagaac cgcaagtagt aatagacgaa gcattgagag atattggcgt
     5341 attcgttgtg tctaatgaaa aagaaccagc tgcaaatgag aaaaagccga caatctataa
     5401 tcatttcatg aatggtgtga attacatgct tccatttgtg atcgcaggcg gaatcatcat
     5461 cgcactgagt tttgcctttg ggattacagc ggctgatccc aactcagcag actataatgt
     5521 gttagcagct gcattttcaa ggatcggagg agatacagcg tttgccatga tggttcctgc
     5581 gttaggcgcg gggattgcta catcgattgc tggaaaagca ggttttgccc caggaattgt
     5641 tgccggatta ctggcctcta caggtggttc aggttttctt ggcggaatga ttggtggcgt
     5701 gctagcaggg tatatctgtg attttctggc aaataaaatt agcgtaaaaa aagaattttc
     5761 tgctatttat cagctgattg tcgtcccctt tttatcgatc ttaattattg ggcttttgat
     5821 ggtatttgtg attgaacaac cgatcacatg ggtgttggat gctttgacaa attggctgaa
     5881 tagcttggga aatacatccg ggctattgtt cggattactt atcgggatca tgatggcagc
     5941 tgatatgggt gggccgatca ataaatcgac ttcgactttc tcgatcggtt tgatgtcagc
     6001 tggagtaaat gcaccgattg ctgcttgtat ggctgcagga atgacacctc ccctaggtat
     6061 tgctttagcc actttattat tcaaacataa atttaccaaa gaagaacgga cttcaggtca
     6121 atcatgctgg attttaggcg cttcgtatat tacagaaggt gctataccgt ttgcggtatc
     6181 tgacccgatc cgcgtgatac ctagtttgat gacaggttca gcagtcgctg cagggatttc
     6241 catggcagta ggagttactt caatggcacc tcatggaggg atttgggtga tgctaattcc
     6301 aaatgttatg aacaatttac ctatgtatgt cttggcaatt gcatcaggta cagtcactac
     6361 agcctttatg cttggactat tgaaaaaaac agtgaaagag aaaaaacaag acaagtggac
     6421 gaaaaatgaa aatctaacaa atgaggtgga agcgtaatgt tatatacaat gaaagagtta
     6481 ttgaaagtgg ctaaagagaa tcaatttgca gtaccagcct tcaatatttg cagctatgat
     6541 atgatgaaag caatcatcga agaagcagag agattagaag caccagtaat cttagaaatt
     6601 catcctgatg aaatcgccta tttaagtgat gagtttgttg cggcagtaag agcctatgct
     6661 tatacaagta aactgccgat cgttatccac ttggatcatg gcggtacgat agaagatgtc
     6721 ttacgggcga ttcgaaacgg ctattcttcc gtgatgatcg atgcgtcttt gaaatcattc
     6781 gacgacaatg tacagattac gaaaaaagtg gttgcgttag ctcatgcggt aaatgtctca
     6841 gtggaggctg agcttggaac gattgggaat aatggttctg ctgaaggagg tgctgaaaca
     6901 atcatttata cagatcctga tcaagcgaag atttttgtga aagaaacagg aatcgataca
     6961 cttgctgtag caattggaac agcgcatggc ttgtatccga aaaacagaac tccaaaatta
     7021 aatatcgact tactgaaaga attgaaccaa caaatcgatc ttccatttgt tttacatggc
     7081 ggttcaggta atccggacgc agaagtcagc gaagcagtaa aatacggagt tgccaaagtg
     7141 aatttgagct cagatcttaa aagcgtcttt ttcaatgctt tgagagaaat attgagtgaa
     7201 aatccatcga tgtacgagcc gaatgcaatt tatccagcag ctaataaaaa ggtacaagaa
     7261 gtggtcaaac ataaaatgga aattttgaac acagtaggca aggctagttt atactaaaaa
     7321 taaaaatgtg tggtcaagag ggtatgacaa tagtcttgtc ataccctctt aaccacacac
     7381 gatgttcaga agtagttttt tcgaaaaatt tcttcttatt tctcgaagcc aatcacttct
     7441 gtcacaacct cttttttcct gttactatat aaagtaaatg gggtgatttt atgttgaatg
     7501 aagtggagaa tagacagcag gagattgtga attatctaaa aaacaaccaa tttgcaagag
     7561 tcaccgattt gatcgagtta gtaaattata gcgaagccac gattaaacgt gaccttgttt
     7621 ttctggaaaa aagtggattg atccgtagag ttcgcggtgg cgccatgctt attgacaatc
     7681 gaaaaattga cgtgccttac ctgatgaaga tcaccaaatt agaagaagaa acagaaaaac
     7741 gtaagattgc agatacagct tcttctttga ttcgagatga tatggtctta ttcttggatt
     7801 ccagtacaac ctctttacac ttagtcagaa acctagcaag atttgatggt ttgcagatca
     7861 ttacgaatgg catcgtcaca gctgctatgt tgagtgaata tacaacggca aaagtatctg
     7921 tattgggagg ggcaattgtt ccgaaacgag caacaatcaa cggttcaaaa gcctataacg
     7981 atgcattgtc atattatgcg gatttagcga taatgagttg tcgcgggcta gatttcagcc
     8041 aaggagtaac agaaactcat gaaggagagg caatggtcaa aaaagctttt agaaaacaat
     8101 cagaccggct tgccgtttta gttacacagc aaaaaattgc acataaattc atgcatcaag
     8161 ccattgcttt tcatgaaatc gattatctgg tgacaagtaa agaactgaca aaagaacagt
     8221 atgcgaaagt aaaggaacac catatcgaat gtctgtacgg ataaattatt acgtgaatgt
     8281 tctaagtggc ttcctcattt ttagatagga ccttatcttt gctcgcttct ctcagccgat
     8341 ggtataataa tgaaaaggtt atcttgttag ataatcttac ttttttatcc aggagaaagg
     8401 agtgcagaaa atgaaacatg tgaaggcgtt ggtagtgaag gcaatcatga tctgggcgat
     8461 tctgtgggtc gtgctgacag gtttatacgg tgttagcttt atggattcaa cgattgttgg
     8521 ggtaattatt gtagtcatga tctatgtgct aggggatctg ttgatcttac gtaaagtagg
     8581 gaacatcgca gcgacgatcg cagatgctgg ttcagctgca gttatcttgt ggctctatct
     8641 atattttatg aatgatacag tcgatatttg gatgaaagta ttgattcctg ctctattgat
     8701 cggtgtggca gaatggttct tccacaagtg gttgctaagt caaggcatcg taccagatga
     8761 acggaaaatg gaataaatca acagttaaac tattcttatc gataaatgaa agctttaaaa
     8821 ataaatagat gtttaattaa gaataatcat aaactatttt tactcaaaaa accgctcaga
     8881 ttattttcag atctgaacgg ttttttttat ctattcatca agatcaaatt tcatttttca
     8941 atgattcaat caattcgccc tctgcttcat gactatgttt tcggccatta acaagataga
     9001 gtgctgtaca tatcaatgga atgactccgg caatcacata caatcctcta aatgaagtaa
     9061 gagattttag catccccatc aaataaggac caacgcctaa gcctaggtct aaaccaataa
     9121 agtaggtaga taaagcaata ctgattcgat gttcgcggac gatttttaaa caaacagcct
     9181 gtccatttga cataaacgta ccataaccta ggccgactaa tgctccagag ataagcaatg
     9241 tcacactatt cgtcgttaca ctcagtaaaa ataatcctgc tgttaagaat aaatagctag
     9301 gatacatcac gtattgttct cctttgacat cgaaaatcct tccagtcaat ggacgtgtaa
     9361 atgtgatgac caaagcatat acgacgaaga aaaatgaccc agcagcaact aagtggatta
     9421 cttttgtata agaagataaa aatgataaaa cacttgaata agaaagaccc atcaaaaagg
     9481 caattcctgt aatgaaaagt gctttctttt caataaaact attgatattc catgagcgaa
     9541 gagatatttt ttgttcttca gataatggaa tattttttac aggaaagaca aggcaggcaa
     9601 tcgtaacaaa gaagaccagt acaattgaaa accagataat gaaacgaaaa tcagtcatat
     9661 tcagcaaaat cattcctaaa aatggcccaa cagcggcagc taaactagta cttaatccat
     9721 aataattgat gccttcccct tttttagatg cagggatata tgcagtaacg atagcatttg
     9781 tagctgtaga aactgtaccg tagccgaatc cattcaagaa tcgaactaaa tacaatgccc
     9841 cgatagttgg cgtataaaga tatgctaatg ttgaaaggag atagaagatc gcgccgcctc
     9901 ttaaggtaaa cttccgacca aatagttcta actgtttccc catgaataag cgtgccagca
     9961 atgttccgat gatatagatc ccagaagcaa aacctgcttg gctcaagctg gcgtgtaagg
    10021 tgtcttgcgc aatgactgcc atgatgacca tcaacagata ataaatcagg tagacaataa
    10081 aattgatcaa tgtgattcca ataaaatatt tattaaataa tttcttttcc atgtatgtta
    10141 tacattcccc ttaagctgaa tatgctttta atttttgtat ttgaacttct acatcgtgat
    10201 aattggatgg atagcgatca acaagttggc gaataaataa atctcttgta gacataaaca
    10261 gatttaaaaa ttcttcgcat tcgtttgttg gattctcatc cactgctttg atcaattcga
    10321 tcgagttttg atacgcatca tttaaaaaga ctaaagaagc tttcatatca aatcttgttt
    10381 gatatgctgt ttctaatcga ttaatgattg tgttcatcca gtaattcatg ttttttccct
    10441 ctattcttaa aaaattttac ttctgcagta taacgaataa aaaaaaggaa aaaaatcgca
    10501 taagtgatgg aaaaaggtac ttaacaaatg ttaatatcat tagtaaaata cctttggact
    10561 ttaaaaagtt ttaaagtact gtatgatctt tttagatgaa agtttggatg ggggagcatc
    10621 attggatagt agagtcttaa aggaatattt ggataaaaat gattttccga ttatacgcaa
    10681 aggatatcat aaatatttga cattcgaagg gatggaagat tcccatacgt atatcttgaa
    10741 agagggaacg gtaaaagcaa gtgttatttc aaatgatggg agagaattta accttcgcta
    10801 tatcaaagat cttgaaatcg tctctctttt aaaagacgaa tattcacaat tcatcgattc
    10861 gccgtataat attcgagttg aatcagaata tgcagagttt tatcgaatca atcgtgtgcg
    10921 cttttggcaa aatatcaacc aagacccaaa gctacaacac tatgtaaaag agtattaccg
    10981 ctatcgtctg ctttattcga tgaaaaaaat gcagcaaatg ctattaaacg gaaagctggg
    11041 agctgtttgt acgcaacttt acgaattata tgatttattc ggtgtgcgga cagaagaagg
    11101 gatgctgatc gatttttcga tcacgaatga agaaatcgcc aaattttgtg gcatctcaac
    11161 tgctagcagt gtaaacagaa ttttaaaaca actgaaaaaa gatggtatta tcaccgttaa
    11221 agaccatcat atcgttattt tgaatatgga cttgttagaa gacaatatta tcttttgatg
    11281 aacaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggaaaaat
    11341 agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt
    11401 ttaccaagag gttgtgagcc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa
    11461 cagcttttca tgtttgatga atattgtgac ttgtgaccga ggagttggta ggagaacacc
    11521 gtttaccaag aggttgtgaa aaagctgttc tgcatcaagt aataagaaga gccaagtaaa
    11581 aacagtctct tatgttttta acttggctgg acttatcacc gtagatgtaa gcttttgaac
    11641 gccgtttatt tgcaacaaaa ggggctgtga cacgattttt gtcacagccc tttttttgcg
    11701 aacattcatt tagcaatttt tagaaacttc tgccgccacc gccaaaagtc ccgccaccgc
    11761 tagaatgggt agtacttccg ccaccgccgc ttccaggagg tgggcttttg gggattcttc
    11821 ttgtcgtgac aaaagaattt gtcagacgat cggttttttc agttaatttg atactggatt
    11881 tttctcggaa tggatatttg tagatgcctg atttcaattg ataacgcgaa acagtcacaa
    11941 aataaaagac aagactaagg ataagagcca aaacaacagc aatgatgatt tctactgtcg
    12001 tcaaaacctt atagcgtgta attttgccag tttcttcgtc tacacgataa tgtcctcctg
    12061 gaactccgtc ttccacatat gttgctgatt tagacaggta agcttttgca gcattgtaat
    12121 aggcagcatt tgtcatctga tcataaacat catctaagat cgaatcgatt ctgctatcag
    12181 tcaaataatc gatcatatta ccagaagtcg aaatgtagat ttcacgctgt cccatatcta
    12241 atagcaatac tgagccattt tgatcatttc cgactgcgtc tcttaagtaa ttatcagcaa
    12301 agtctcttgg ttcttctgaa ttagatgtag tcgttacgat caagactcgt cctttgattt
    12361 tttcattcag cttttctgcc tgttgttcca aagattggat ttgatctgtt gtgaataacc
    12421 cagcctcatc atttaccgta gctgtatctg catgagtagc caccggccag ccaacactta
    12481 ctagcaaaag aaagaataaa aaaaattttt tcatcatatc aagtaacctc cgatcagaaa
    12541 gaggatcgct agcccagcga aaatcccaag agcggtcagt cccaattttt tctggctgat
    12601 cggcaggaca ccgctgacct ttcctgtttg tccattcatt gcgtagtagt agactttttt
    12661 acttgagtca ttgcttcgat aagtaacaag ccataccggc agtaatacgt aattatttac
    12721 ttcgtttgtg attgaagcag aagttcgcac accggtcaat gttgtatatc catttgcggt
    12781 atcttttaac agcttctctg aatagtcttt taattcagat tgaatctgtg atttgattgc
    12841 ctcatattcg atatctcttt tttctgcttg gaacccggct aaatactggc ttttaaaatc
    12901 aattgcttta tctaacggga aaggttgcac gccttcgacc atcttttgtt gcgtattttt
    12961 tgataaggca ttttttacta gttctttaaa agaaagcttg cctgctcttg aaacagcaaa
    13021 ttgcttcgtt tctgtgtatt cgatgtcacc tatccgccat atgcggataa ctgtaccatt
    13081 cgcctgcatg gcaccatcta cttcagcatc tgtagcccaa taagggaaat acacacctgt
    13141 caacttatcg atttgatcct tattgaaaaa agctttggga acaaaccatt ttttcttggt
    13201 ccaagcgaga aatctgttga ttgcttcttc tttctcgaca gcaaaaggaa gtactttatt
    13261 tggcaagaat tctccgctta aacgtcccga tagtacgacg ggattatggc agtagtaaca
    13321 ataagtcgca gcagttgtcg catcagtcac gatctctgca ccacagttgg gacaaagaaa
    13381 aagatctatc gctgcggtat tatctgcaga ttcgtccgct gtatctgaaa aaccgcctgc
    13441 ctcgtcaacc gctttgcgct cggcttcatt catatcggca agctgttctt gggctgtaaa
    13501 agtaaaatct tcggtaggtg aaggttcttc tttgctttct tcgccagcca tacgcgcttc
    13561 tttttgcttt tgctcatatt ggctgacttc ttcttctgta tagatattca ggcagtattc
    13621 gcaatgaaat ttttggtctt tgggatcaaa agtcaacgga ccgccgcaat taggacattt
    13681 atgtgtaaga acatccatca gctgcacacc tttctaaata aattaattaa cggggattcc
    13741 tttaaacggt ttaccacagt ttggacagaa tttagggatg ccgttggaaa gatccacgat
    13801 ctcattgcat tcactgcatc gcatttgtag cttaggctga acgttttcta caggtttggc
    13861 agctccgcag ttggaacaga attttccgga attttctgtc ccgcaaactg gacatgtcca
    13921 agtatccgca tttccttgtg cagtttgttg attttgtttt tctgcttgtt gtttgatttg
    13981 ttcttggtta ttttgagctg cttgagaaag ataagaacca tttgcattca ttcccatacc
    14041 tacacccata aagccggtca ttgctcctgc ttcatttttt ccggctgctt ccataccacg
    14101 tgcaatggaa ccttgcacgt agccttcacg tacgctagga tcacctagca tcgccccttc
    14161 gttacgcata ttgatcagtt tcacagaatc atcggtgtaa gaaatgctgg ctacagccac
    14221 agaaacaatc tccatcccac gtaattcacg ccaagaatca tccaatgcag tatccatgta
    14281 tttgcttaat tccaaactct tagatggtac ataagaaatc cgttgaccat ctgcagacat
    14341 ctgattgatt gctgattgta gcgctgttag gaattcagct aaatactgct cattgatatc
    14401 attgatttcc acttgtgttt tgtttttagg gattgcgttt gtataaaata aaatcgggtc
    14461 aacgacatga atagaataag tgccatgtgc ccgtaagaat aattctgcgt tatagaaatt
    14521 gtcaaagtag ttcaacggcg cgcttgtacc gaatttgatt ccctttattt cttggagatt
    14581 gatataaaaa acttgttgtt tctgcggtgt gacgccgcca aatttgaatc ggtcaaaagt
    14641 ttcagcaatt gcttctttca gtgttccatt gaacatggaa ggagctgcgt catttttaat
    14701 ggtgtaataa ccttcctcag cggtataatc gatgattttt ccaccatcga ccaaaagcat
    14761 catcatgttt ggatagacat ggacgactgt tccgtctgtt aaaacatctt ctgttccttt
    14821 acgattggag ccacgtttgt catctttgcg gactttgaca ccttttgtca tgaccgttgt
    14881 atcactcata ttgtctggtt cgatgacttc tagccattga tctgccagac cgccgccaac
    14941 catacttgtt gctgctttga taagacccat aaaaaattcc tcctctgatt cagctagtgc
    15001 caaacaatta ttgtttattt attataccat aggaagatgt gaaacttctc ggactagaga
    15061 cggaattgaa atggaaaatt gcgtattgtt taaatacctg aaaaagccga gaaggatagt
    15121 agttttttta cctaatatca cgtataataa gagatagtga attttttaaa aaagggggat
    15181 ccttatggca tatcaagcac tttatcgagt ctggcgttcg cagcgtttcg aagacatcgt
    15241 tggacaaaaa gctgtgacac agacactaaa aaatgctatt gtttcccaca aaacctcaca
    15301 tgcctacttg tttacaggac ccagaggaac cggaaagacg agtgcagcaa agattttcgc
    15361 aaaagcaatc aactgtccga acagtaaaga cggtgagcca tgcaacgaat gcgaaatgtg
    15421 tcgatcaatt actgctggta cgcaagaaga tgtgatcgaa atcgatgctg ccagcaataa
    15481 cggagtcgaa gaaatccgat ttatccgtga tcgggcaaat tatgcgccta caaaggcagc
    15541 gtacaaagtg tacattattg atgaggtaca tatgctttca acaggagctt tcaatgcctt
    15601 gttgaaaaca ttggaagagc caaaagaaag tgtcatattc atcttagcaa cgaccgaacc
    15661 tcacaaaata ccagctacga tcatttcacg gactcagcga tttgatttta aacggatcaa
    15721 tacggcagat attgtcgaac atcttgaatt tatcttgaaa gaatcgacta tcagctatga
    15781 accagctgca ttgaatgtga ttgcccgttc tgcagaaggc gggatgcggg atgcattgag
    15841 tattcttgat caggcaatct cgtttagtga aggaacaatt actttagatg atgccatgca
    15901 agtgacaggt agcttaactg acgaaatgat ggacagttac ttgagtgctt gcgtgaaaaa
    15961 agaagtacca gaagcattgg aaacattgaa ccaaattctc gcagccggca aagaatcacg
    16021 tcgctttcta gaggatatgt tacaatactg ccgtgacttg ctgatgtatc agcaagcacc
    16081 taacctagtt gaggaacaat cgagtacgat cagaagtaca tttacagaat tggcacaaac
    16141 tgtttcagcg gatcagctgt tttcaatgat aaagattttg agcgatactc aaaatgaaat
    16201 tcgttttacc aatagtgccg ctatttattt ggaagtcgct acagtcaaac ttgcacagct
    16261 accgtctgct tctgttcctt cagcagatgt agcatttgca gatgatcaga tagtaaaaca
    16321 gctacaacaa gagattaagg aactaagaaa cgaaatcaag gaactacgca gtaatacagg
    16381 aattgagaat acaaaagtac agccggtaca gcaaacaaat cataaaaaac agacaagtac
    16441 ctatcgtgta ccgacggaac gagtatataa agttctgcgg gaagcgacaa gacagcatct
    16501 gaaccaagta aaagaagtat gggaagactt gctgatgagt ctatctgtca ctcagcgagc
    16561 aatgcttaaa gcaagcgaac cagttgcagc tggacctaca ggactagtga ttgcgtttga
    16621 ttatgagatt gtttgtcaaa gagcagcaaa tgacgaagaa ttgcagcttt cggtacacaa
    16681 caacttaagc cgaatgatca aagattatgc gcctgatcct gtttatatta caagagaaag
    16741 ctggccagaa cttcgaagaa attatttgac gcaggcacaa gaagagggaa cagaaaactc
    16801 ttttgcttct gaagactcag aaggagaaat cgagttgctt cctcaagaac aagaagtacc
    16861 agaagaaaca cttgtagtaa aagcagagga actgtttggc tccgaagctg ttacagtggt
    16921 agatgattaa taaaaaggag tagataatta tgatgcgtgg aatgggcaat atgcaaggca
    16981 tgatgaaaca agtacaaaaa atgcaaaaag aaatggcaga agcacaagag ctgctaaata
    17041 aaacagagtt tacaggtaaa gccaccaatg aattggtcaa agcggttttt acaggcgatc
    17101 gtcgcatgaa agatatccaa atcaatgaag aagtcgttga tcctgatgat atcgagatgc
    17161 ttcaggatct tgtcgtaatg gcagtcaatg atgcgatcgc acaaattgat gtggaaactg
    17221 agaaaaaatt agggaaatac gcaaaaggtt tgccaggatt ttagttttaa actgaaggag
    17281 taatgaatat gcaatatcca gaaccaattg cgaaactgat cgacagctac atgaaacttc
    17341 caggaatcgg tcagaaaaca gcgacaagac tcgcctttta tacgatcgat atgaaagatg
    17401 aagtagtaaa tgaatttgct aaatctttgt taagtgtaaa acgcgatttg catttttgta
    17461 gtatctgcgg aaacatcaca gaagaagatc cctgcgaaat atgtagaaat ccttcacgtg
    17521 atcgaagcgt tatcctagtg gtcgaagagc cgaaagatgt aatggcaatg gaaaaaatgc
    17581 gagaatatca cggactttat catgttctcc atggtgtgtt atcaccgatg gaagggacag
    17641 gtccagaaga catcaatatt tcttctctgt tgcagcggct gcatgatgca gatgtaaaag
    17701 aagtgattat cgcaacaaat gcaacaacag agggagaagc aacagctatg tatctctcac
    17761 gtctgatcaa acccgcaggt atcaaagtga ctcgtctagc acatggtctg tctgtaggct
    17821 ctgatatcga gtatgcagat gaagttactt tattgaaagc tgtcgaagga agacgtgagt
    17881 tataaatttc taattaagtg ttttttcaga tacaatataa aagcagcaca agagatgggg
    17941 gcaacaatgt gaacggacta tttattacaa tcgaggggcc tgatggtgca ggaaaaacca
    18001 gcatcttgaa tgaactttac cctcttttga aagaagtagc aaaaacagaa atcattcaaa
    18061 caagagaacc aggaggcatt ccgattgctg aagagatccg agcagtgatc cttgatccaa
    18121 aaaatgatcg gatggatgaa cgtacggaag cactgcttta tgcagcagca agaagacaac
    18181 atctagtcga aaaggttctg cctgctctgg caaaagggaa aatcgtttta tgcgatagat
    18241 ttgtcgacag ttctcttgct tatcaaggtg caggccgacg catcggcgtt accgagattg
    18301 ctcggttgaa tgagtttgct acagaaggta ctacgccgga ttttaccctt tatcttgacg
    18361 tagattcaga caccggatta agaagaatca agaaaaacag acaaaatcaa atcgaccgtt
    18421 tagattcgga aggtttggaa ttccaccagc gtgtacgtca tgcttactta aagctggcag
    18481 aagagaatcc ggaaagaatc cacaaagttg atgcaagaaa aagctttgaa gaagtgctgc
    18541 aaacaagtta tcatacaatt attgaacagt atccgcaatt ttttgaaaac taggaaggtg
    18601 aacgtttatg aaaattattt tagcgattat ccaagacaaa gacagcaacc gtttatcaaa
    18661 tgaattgatt gatgcaaata tccgtgccac aaaactatca tcgacaggcg gcttccttaa
    18721 ggctggaaac agtacgttta tcgtgggtat tgaagatgac cgtgtagaag aagcattgga
    18781 aatcatcaaa aaaacgtgtg aatcaagaaa gcaatttgta tctacacctg ttacactgga
    18841 tatttcgatg gatgggggcg tcccttatcc agtagaagta gaagtaggcg gtgccacagt
    18901 cttcgtctta cctgttgaag gattccatca attttaggag gagctgagct atgaatcaag
    18961 aaataaactt ggctcaaatg caaccgatcg tgtatcagca gcttcaacgg agttttgagc
    19021 atgggcgtat cgctcatgct tatctttttg aaggggagaa aggaacagga aaacatgaag
    19081 taggtatatg gcttgcacag cacctgttct gtacccaaat gaaagacaat ctgccttgtg
    19141 gaaaatgcaa caattgtcag cggatcgaaa agaaagaaca tccagatgtt ttggttattg
    19201 aaccggaagg acaaaccatc aaagtcgatc agatcagacg tttgcagacg gaattcagtc
    19261 gcagcggtta cgaatcaaga aaaaaagttt ttttgatcaa agaagctgaa aaaatgaata
    19321 gcagtgcggc aaacagtttg ttgaaattct tggaagagcc ccctggagat tttttagcgg
    19381 tgttagaaac agatgcagta ggaagaattc tccctactat tcaatctcgt tgtcaagttc
    19441 tgcattttca agaactttcg aaagaagcac tgatccatcg attgcaagaa gagcagattc
    19501 cattggaaaa agcaaaactt ttggctttct taacaaacag tttaggaaaa gcagttgaaa
    19561 tctctcaaga tgaatggttt aatgatgcta aggacttgat ttatcaatgg ttcgtttact
    19621 tgcaaaagca agatactcaa gcctttatct atgtccaaaa aaaattggta aaatcattta
    19681 aagaaaaaac acaacagttt acaggattat ctgtcttgat gttttattat caggaagcac
    19741 agcgtcaagc agtagcaaac ggcgaatatg gcaaagtgaa acagataaac cagataattg
    19801 aacggatatt gatcgcagag cagaaactac gcagcaatgt cagttttcag gctgttgcgg
    19861 aacagttcgt tttgcatacg attttttagc ttgccgcaaa ggatgatgat caaggaggat
    19921 ggaaatggta gaagtagtag gtgtccgata tagagatgca ggtcatatct attattttgc
    19981 accaggagac tctgaatatg tttatagtga caaggtatta gtcgaatctc agcaatcctt
    20041 acatattgct actgttgtcc ttcctaaaaa agagattgat cccaatgacc ttcctgaagc
    20101 tatccgtccg atcttgcata aagcgtcaca agctgaatta gaaaaagaca aaaaaaacaa
    20161 aaaagatgct tatgatgcat tgaaaatcgc aaaagaaaaa gtgcagttgc gtgagttgga
    20221 aatgaaaatg gtcaaagccg aatacacatt tgatcgaaat aaattgattt tttctttcac
    20281 agcggacggg cgaattgact ttagagaatt ggtaaaagat ctggccgctg tatttcgtac
    20341 tcgtattgag cttcgacaaa ttggtgtccg cgatgaagca aagataatag gcggaattgg
    20401 tccatgtggg cgtccattat gctgttcttc ttttttaggc gatttcgtgc ctgtatcaat
    20461 caaaatggca aaagatcaaa gtttatcttt gaatccaaca aaaatttccg gtttatgcgg
    20521 acgcttgatg tgctgtctgc aatatgagag cgaagaatac gagacagcga aaaaagaact
    20581 ccctgacttt ggaaaagtaa ttgctacacc tgatggtaaa gggaaagtcg tagggttgaa
    20641 tctgcttagc cgtatcgtta gtattcggct gactggacgg gaaaatcctg tcgaatatga
    20701 atgggatgaa ttgaaagaat ttatccaagt aggtgagatg aatggataag agatcattat
    20761 acgacggatt gaatcaatta gaatcagagt tgagaaatac attaacagaa cttgttgaaa
    20821 tgaagcatgc tttgcatgag atcgtagaaa aaaatacaac tttggagatg gaaaatcaac
    20881 gtctgcggga acacctgcgg gaattgaacc aagttgcaca agctccagct gaaaatgtga
    20941 agcaggaact ttctaagtct cgtatgaatc ttgaaaaaat ttatgaagaa ggattccatg
    21001 tctgctacga tttatatgga tcaagaaggg acaatgacga gccttgtgcg ttttgtctag
    21061 aagtgatcta tcgcgaaagc ggtcgatgac caataacaag aggagactgg agcggagaaa
    21121 tactgctcca gtctttttat taaggagatt gtgacagatg tataatcaaa aaagtttcaa
    21181 gggaaatccg gtatatgggg atttatatct tgtaccgacg ccaattggaa atttagaaga
    21241 tatgagctac agaagtgttc gtatgctgca agaatccgat cttatcgcaa gtgaggatac
    21301 gcgtaataca caaaagttat tgaatcattt tgaaatccat acaccccaaa aaagtttaca
    21361 tgaacataat tataaagaac gtgtccctca attgattgct ttgttaaaag aaggaaaaac
    21421 gattgctcaa gtcagtgacg caggaatgcc ttccatcagc gatcctggac atgagctagt
    21481 tttagcctgc ataaaagaag ggatttctgt gatctctatc ccaggaccta cagctgggat
    21541 gacggctttg attgcttcag ggctagtgcc tcaaccattt ttattttatg gcttccttcc
    21601 acgtaagaaa aaagaacaga aagaaacatt gaaaaagcta aaagaacaga cagttacgct
    21661 gattttttat gagtcacctt atcggatcac tgctacattg acgaatttct tagaaatttt
    21721 tggaaatcgc caagtcgttt tgtgtcggga attgacaaag gtccatgaag aataccttag
    21781 aggaagtacg gaagagctac tcgcttatat cgaagaacat ccagtaaagg gtgaatgttg
    21841 tctgcttgta gaagggaatt tgttcgaaat gccagtgacg gaagttgatg aagagttggg
    21901 aacgttaaag gaacaagtag aagcaaagat cgcttcaggc gaaaaaccaa atgctgctat
    21961 caaagcagta gcgctgaaaa atgggctgaa aaaacaagaa gtctaccgtc agtaccatga
    22021 attagactga gatagtataa gttgtctata atgaaagact aatgaggaaa tcagctacta
    22081 acttttcaaa tctcttgaac gcgaacgaat gttcttgtat aatgaataag aggtgaggat
    22141 gatggagctg caatttcctt tacataatga tacatcaaga aaaatcattc atattgatat
    22201 ggatgctttt ttcgcttcgg ttgaagaacg agatcatcct gagtttcgcg gacatccatt
    22261 agtaattgcc agacacccca gtgacactgg tgggcgtgga gtagtgacta cagcaaatta
    22321 tgaagcaaga aaattcggaa ttcattcagc aatgagtgca caaaaagcgt atgagctttg
    22381 tccgcaagcc atctttaagc ctggcgacca tcataaatac agcgatattt ccaaacaagt
    22441 acgggaagta ttctatcgtt atacagatat tgtggaacca gttagtatcg atgaagcgta
    22501 tcttgatgtg accgaaaata aaatcaattg caagtcagcc atcaaaatcg cccgaatgat
    22561 ccaagcagat atttgggaga gtgtccagct aacatgttct gcgggagtaa gttacaataa
    22621 gtttttggcc aaattagcat cagactatca aaaacctaga ggaatcacgg tagttgcccc
    22681 tcaagacgct gtgccatttt taaaagcttt gccaattgaa aaatttcatg gggtagggaa
    22741 gaaaactgta cctagaatgc atgagctagg gatttataca ggaaaagacc tatatgaatg
    22801 cacagaaatg atgttgattc gaaattttgg aaaaatgggc tattctcttt atcgtaaagt
    22861 cagaggaatc catgattctc cagtaaacgt tacaagagag cgaaaatctg tgggaaaaga
    22921 gcatacttat gggcagccgt tgcaaacgga agaagcagtg ctgactcagt tgcgccaact
    22981 agctgaaaaa gtagaagaag cactgagaag agtccaaaaa catgggaaaa cagtagtttt
    23041 gaaggtcagg tacacggact attctactgt gaccaaaaga gtgacattgc cagaatatat
    23101 ctataaaaaa gaagcactct tttatcaagc aagccttata tgggaggaaa tcttgggagt
    23161 agaaaaagga atccgattgt tagggattac tttgacgaat ctggacccga tgacttatga
    23221 aaacatcgtc cttccattat gggaaaatca agaaatataa aaacagcttt gagagttgaa
    23281 accggaaatc cgttttctct cccaaagctg tttttatatc aagtcttgct ttttgatttg
    23341 ttcataggtt tctgttgcat ccgtacattt ttcccaaatc acaacttgat tggattctaa
    23401 agatttttgg acgtcaatga tccgctggtt gctacttccg cgaaattgta gttttaggtc
    23461 acgtttgctt aattcaaagc gtccgtcaac aaggatatcg atttgcgaaa ggagttctaa
    23521 tttatcttct gaatcttgta gcaattcatc aaaggtatag cctgtccatg accagatatc
    23581 ttttgtcgta ccgaactctt ttcggatacg tcgtacgact tgcagacaaa catctgtatt
    23641 caaaaaaggt tcgccaccaa gtaaagttaa tccttgcacg taatcgttgc gcaagtcagc
    23701 gatgatttgt tcttctaatt caggtgtgaa tggtttgcca tatcgaaaat tttgtgcagc
    23761 tttgttgaag cagccttcgc aggcaaataa gcagccacta acgtaaagac tgttgcggac
    23821 accttcacca tcgacaaaat taaacggttt gtagtcagca atatagtttt ggctgtagtc
    23881 ttttgctaac cattcttttg gttttggatt tctcataaaa aaacttcttt catttaaggt
    23941 tcaaagatat agctattgaa gataaaatct actcgaccac ataaataaga acaaactgac
    24001 ctaaatccat tgttcaagaa agcttgttct tattttgata gagggcgttc aaagatacaa
    24061 agccatcgaa agagggtggt gaaaaaagac ttgtcatagc ctcttattct gaataaacgg
    24121 tgttcaaaag cttgcatctg cagtaataag cccaactaaa cgaaaaatat gaggaactat
    24181 ttttgtttag ctattcttat tacttgatgc aggacagctt tttcacaacc tcatttcatg
    24241 tgtttcacac gtgaggaaat ttctttatgg cggccatgta ccatcggacg agcttgcgga
    24301 tttcctaaat agccacaagt acgtttgact acatcacatg tttttggatc atgatttcca
    24361 cattctggac attcgaaccc tcgttctgtc ggtttgaaat caccttcgaa accacattga
    24421 tagcaatgat cgattggcgt attcgtacca agatacccga ttttgtcata tgcaaaatcc
    24481 catacagctt ctaaagcttt cggattttgt tggagcatag ggtattcaca gtagtggatg
    24541 aatccacctg aacaatattt tggataatcc ttctcaaatt ccaatttttc aaatggcgtt
    24601 gggtttttac gcacatcata gtggaaactg tttgtataat attctttatc tgtgatattt
    24661 tcgatgatgc cgaatttttc tgtatccaaa cggcagaaac ggtcagttag actttcactt
    24721 ggagttgaat agacactgaa atgataacca tattcatccc cccatgcatc tgcattagct
    24781 tttaattctt tgacgatatc aagtgtgaaa gcttttgctt ctgggtttgt ttcccattcg
    24841 ccaccgaaga aagctgaagc aacttcatac aatccaatgt agcctaacga tacagtagct
    24901 cgacggtttt tgaataattc attgacagaa tcggtacgtg aaagacgttt cccgaaagca
    24961 ccatacatat ataaaattgg cgcatttgct ggaatggctt ctttacaacg ttctacacga
    25021 tagagaagcg catctttcat gatttctaag cggtcagaca gtaactgcca gaatttattc
    25081 tgatctcctt ttgattcgat cgcgattctt ggcaaattca atgtaacaac acctaaattc
    25141 attcgtccga catttacttc ttgaccattt tcgtctttcc acccttgcaa gaatgaacga
    25201 cagcccatag gtactttgaa acttccagtt aattcaacga ttttatcgta attcagaata
    25261 tcaggataca ttcttttcgt tgcacattcc aatgctagtt gtttgatgtc gtaattagga
    25321 tcttcagggt ttaagttcac accttttttc aaagtgaaga tcagtttagg gaagatagct
    25381 gtccgctttt cactccctaa tccattgatt cgaatctgta aaattgcttt ttgaatttct
    25441 ctttcaaacc agttttctcc tagtccaaat cctaatgaag tgaaaggagt ttgtccattt
    25501 gaagtaaata aggtattgat ttcatattcc aaactttgca tagcatcgaa gatatctttt
    25561 ttggtttttg cttttgcata ttctttttgg cgttctggcg aatcgatcca ttcttccgcg
    25621 tcttttagat gttttttata gttcaattct gcaaaaggag caagcaattc atctgttcgg
    25681 tcggcagaac agccaccata ttgacttgac gctacattgg caataatctg tgagatttgt
    25741 gctgtggcgg tctggataga ttttggcgat tcgacttcgg cattgccgat tttgaaccca
    25801 ttgttcagca tgcctttgaa atcaatcaag cagcaattcg tcatcggtgt ataaggatga
    25861 taatccaaat catggtaatg gatatcgcct ttttgatgcg catttgctat atgtggtggc
    25921 aacattttca gtccgattga ttttccaacg atcccggctg tcagatctcg ctgtgtattg
    25981 aaaacattac tgtctttatt tgcgttttcg ttcacgacag tttggtcctt gttgattagc
    26041 ttttcaatcg aaaagttgat atctgttgct ttactgcgag caaaatctcg tttggtacga
    26101 taattgatgt actcttctgc taattcatat tcgttttttt ctagtaatat atgttccacg
    26161 atgttttgaa tctcatagat tttgacattt tcagcaaacc ggcttttgat ttcttggaca
    26221 accaagtctg caatcatttg aattttttca taagtaagtg gtgataatga gccgtgaatt
    26281 ttcttttctg ctttgatcag tgcttcattg atcttttgtt catcgaaatc aacatggcgg
    26341 ccatcgcgtt tgatgacttt catctgggat gtctctggct gctgagtaac gacagttttt
    26401 tcttcataat ttactttcat tatcttcaac tcctgctaaa ttgttcttgt ctatcataaa
    26461 acaaaatcgg ggaaggagca actaaatatc ggctatatat agtattcaat gtgctgtata
    26521 attggatttc tatactatat gtagtgtata ttttattgtg aaaaaaggtg aaaacttgta
    26581 gctggtggat ttgttttttg catatttaat tttgctgaat aaaataattg aataaaattt
    26641 tttattcagt ccaagtaaaa gtgacaaaaa cataagagca aattattcgt agatatgcta
    26701 ctatgcaaat aaatagaaat gaaaaatgaa agaggaggag aagaattgga aaagatgatc
    26761 gaagtgaaac atctaagtaa aagctacggc agcaaacttt ctcccgtcaa agccttagat
    26821 aatatctcat tcacagtaga agaaggcgag tttgttggga ttatgggccc aagtggtgca
    26881 ggaaagacaa cgctgatgaa tatcttgtcc acgatcaatt tgcctacgat gggcagtgtc
    26941 tcgatccaag gaaaagaaat cacaaaaatg aaaaacagtg agctcagtga ctttcgtcga
    27001 aaaaaactag gattcatttt tcaagagttc aatttgatcg ataccctaaa tgcaaaagac
    27061 aatatcctat tgcccctagc agttgaacgg atgactaaag aggaaatgga aaagagagta
    27121 agacatgtag ctcaattgtt gaatatagaa gaactgctca aacggtatcc cgatgaactt
    27181 tctgtcggac agagacaacg aattgcagct gctagagcac ttgttgttca gccacaagtg
    27241 atttttgcag atgaaccaac tggctctttg gactcgaagt cggctacaga attgttgaac
    27301 tacttgaaga ccatgaatca aaaagaaaaa gcaacgatac tgttagtcac ccatgatcca
    27361 tatacagcaa gctactgcga tagaatttta tttattaaag atggtgtgat tttttctgaa
    27421 gttgtcagaa gagggacaag aagagagttc tttgagaaag taatagacat gcaggcgacg
    27481 attggcggag gaggaaaaat gaatgctgta taaactagcc gttaaaagtt tttttcggca
    27541 gtcacgaggc tatcttgttt attttttcag tttgacttta tccgtaatga tctattattc
    27601 cttcagcgca atgacctacg atcaatcttt agtcagaaga gcaagtcaag atacttcgat
    27661 agatggtgga ttgagttttg gtggatttat cattactgtt gttttgcttt ttttcgtatt
    27721 cagtgccaac cgatttttct tgaatagaag gcaaaaggaa ataggcatct atcagctttt
    27781 tggtatgaat aaactgcaga tatcaggtat atatgtgtta gaaatcatga taattggatt
    27841 gttcgcctgt atttctggga tattactcgg tattattttt tccaaactct tttcaatgat
    27901 tttggttcga atgatggata tggatcttac tagtccattt tttatctcca ttccatcagt
    27961 agtagatact ttatttgctt ttttcatcat ccttttagca gtatctgtat attcaatatg
    28021 gaaaatatgg cgctatccaa tgattcgaag ttttggagaa aaagaacaag cagatagttc
    28081 aacaatgcgc ttcaggacaa ggcaccgatt actcgcagtc atcgggttac tacttttaac
    28141 gagcggatat tttggagcta gtcattttag ggaaatcact tcatggatga tcagttatca
    28201 ttatcttgat acattagttt tcttttttcc ttttttaatt tttttcgttt gtgtggtcgg
    28261 tacttatttg tttttctgtt atacatttcg gttatttttg atcatattga gtaaatggaa
    28321 gatttattac tatggtatca attttttgat aatgggtaac acgcaagttc atttgctcaa
    28381 aagttggcgt acaaattctc tgattacagt cattctcgga atttctttaa tgacaattgg
    28441 cggcacggct agtatctcta ctattttatc ctaccaggag aaagtttcct ccccgatgga
    28501 ctatcagttt gatgttgaga cagagaaaaa aatcaagccg attctagcag aggaagggca
    28561 gaagataact aaagaaatca ccttgcatta taaagtcatt gggagtatct atagtcaaaa
    28621 tctcgaagaa cctatgtttc aaactgctaa tctgatcacg gaaacacaat atcaagcctt
    28681 cagaaaagtt aatccagatt tgccgaacat ttctttgaaa tcacctggtc atactgtttt
    28741 gcttgatcaa atgcaaactc tttttcggaa tatttcatta tatggatcta gtattggact
    28801 gccagataat caagaattga agattcaaca aatgcttcca agtgtattag gtgacgaaac
    28861 aatggtatat ctaagtcctg tactgattgt agatgatacc gtttttgaaa aagcaaaagg
    28921 tgtggattat caagtagtca atgtagatgt atcaggtgga aataatgaaa aaatagatga
    28981 acgactgagc aatgacctgt cgataaagtg gctcaatgct gtttattatt cttatgatat
    29041 cgtaaatgga agtctacaag gagaaattag cacaaagaaa cttccggatg accaaatgaa
    29101 ggaagcacaa tttagccaag aaagctctcg tttgaatttc tcttccagct attcgaaatt
    29161 gcgtgcagtt aatcggcgaa tcggtatcaa tatttttgtc acactatttg tcgggatgat
    29221 cgtgattatt gctactggta gtattttgac agtccggcaa ttagcagaag cagaggaaga
    29281 aaaagaaaat tatcacctat tgactaagct ggggattcca caaagaagga tcagacggat
    29341 gatcttcgag caaaacgcac ttaccttctt tccacccatg atattaggga tcactcacgc
    29401 agtatttgca atcaatgtct tcactcagta tataaaaact gccgattact ggttggctta
    29461 tcttgtgagt ggcttattga tccttatcta tcttttgctt tatgtcatta cgagtcagtt
    29521 atattgtcgg attatcgagg aatgatatac ggaataagac aagataataa atagaactaa
    29581 attttgaata ccatttattt ggaataagag gttgtgacac gatttttgtc acaacctctt
    29641 tattttttat attttttaaa agtacgagaa attataaaga aaaaagcagt gatttgatgt
    29701 gctattttta taaaagctat gtgacaaaac tgttagtaaa aaaacggttt ctgtaagcta
    29761 gacacatgtt tctttttgtc aaaattagtt acactttgtg tgaggttata tgaggaggga
    29821 atccaatgaa caaatggcgt tctaatccaa aattgaatta ttggggaaaa ttcatcattc
    29881 gaacaatttt ttatagtgca gtactgctgt tcctgattta tctgtatcac tataaaaata
    29941 ttcaaggagg aacttttatc tataacgaat tttaggagga agagtatgga aatcaaaacg
    30001 attattgaag caatcgatga ttggggaata aatgaacctg atcgagttgc ttatcaggcg
    30061 gatgagacgc atacttttgg tgaattgaaa caagcatcag acgcacttgc ttattattta
    30121 gaaaaaaagg tagaaggcga tggaccaatt gtcgtttttg gcaatttgga gtttgaaatg
    30181 attgtctcat ttctaggggt tgtcaaatct ggccatgcct atttgccgat tgaagagcat
    30241 acacccaagg agcgtatcct atcgatcttt cgggtggcta aaccatctat ggtcatcagt
    30301 atcggggact ggatagaaga tattccttcg gttcctgtca taacaaaaaa agaatttcaa
    30361 gaaatcaaga agataccagt aggattttat cccagaaata gcgtaaaagg tacagaaaat
    30421 ttttatatta tttttacttc tggtactact ggagaaccaa aaggcgtaca aatcagtcat
    30481 gataatctag tcagctttgt tcaatggacg atggaagact ttgggatcaa gcctggtatg
    30541 catttcttag cccaagcgcc attttctttt gatttatcgg tattttctat ttatcctgca
    30601 ttagtttctg gcggaatgct gaagccgtta aataaagcag ttgttcaaga ctttcgtcaa
    30661 ttattcgcga cattgccagc cttgaagttg aatgtgtggg tttctactcc atcatttatg
    30721 gatatttgct tgatggaacc tacattcaat ggagaaaatg tgccggaatt ggacatgttt
    30781 ttattttgcg gagaagaatt gacgaaaaaa actgcagaaa gtctacttga tcgattccca
    30841 aatgcacgga tatataatac ttacggtcca acagaagcaa ctgtagctat ttcaagtatt
    30901 cagattgacc agcatgtact agatagttat gaccgactac cgattggcta cgtaaaaaaa
    30961 gacacgaaag tttctattgt ccaagaaggt aaacctgttt caaaaggaga aacgggtgaa
    31021 atcattattg caggaccaag cgtatcaaaa ggttacctga acaatcccga aaagacagct
    31081 gctgcgttct tttgtcaaaa tgacttagca agttaccata caggggatgc aggtagacta
    31141 gatgaatatg ggcttttatt ttatgaagga cgtatggatc agcaggtcaa attacatgga
    31201 taccggattg aactcgggga tatcgaacat tatctgctcc aagacaaccg aatcaaacaa
    31261 gctgtcgtcg taccaaaata ccaaggaaca aaagtacagc agctggttgc ttttgtcgta
    31321 ttagaaaaca aaacgaaaca gccggatttt cagttgacta aatctatcaa agaacaatta
    31381 ctagaagttg taatggatta tatgattcct cagaagttcc attatgtcga tctgctgcct
    31441 cagacagtta atggaaaaat cgatcgaaaa aaattaacag ctgaggtgaa tcctgaatga
    31501 tgttacctca tatgatccct tatgcagagc cgttttattt ttttctttta gccattgcgc
    31561 ttgtaccgat cgttctatct ttattgatat gggaaaaaag attaccagtt tatcaatcag
    31621 cagtgacact tttcttttta tttgtcagct ttggaggtaa ttattggcaa cagggagcgg
    31681 cgctgattgg gtatgttttg tggcagacga tacttgtatg gggctatttt gtttatcggc
    31741 aaaggaaaaa tcagacagga tggttttttg ccgccgttat tttagcgata attccactgt
    31801 ttttaacgaa agtgactcct ttttttgaac atggaaaagc atcgttactt ggttttttag
    31861 gaatctctta tttaaccttt aaatctgtac aaatgatcat ggaaataaga gatggcatga
    31921 tcaaaacata taagccattt cgttatatcc agtttctgct gttctttcca acaatctctt
    31981 ctggaccaat cgatcgctat aggagatttg aaaaagatca gctccatccg cctgttcgtg
    32041 ataaatatgt tgatcttttg ggaaaaggcg tccaaaatat ttttgtcggt tttttatata
    32101 aattcattat cggttattat tttggtcaag tactgcttcc tgtagtggga cgcattgcct
    32161 tacagcatgg tggtatttca tgggcattag taggttatat gtacgtgtac agtatgtatt
    32221 tgttttttga ttttgctggc tatagcttat ttgcagtcgg tacaagctac atgatgggat
    32281 atcagacacc aatcaatttc aataaacctt ttttatcatg gaatatcaaa gaattctgga
    32341 atcgttggca tatgacgctg tccttttggt tccgtgatta tgtatatatg cgattgatgt
    32401 tcttcatgat gaagaaaaaa ttttttaaaa gcaagatcgt aatgtcaaat atcggttatt
    32461 ttgctttgtt tcttctaatg ggtatatggc atggattgac gtggtactac atcgtatatg
    32521 gactgtatca tgcttgtctg atctgtctga cagatgcatg gatccgattt aagaagaagc
    32581 ataaagagca gttgccttct aacaaattta cgcatggctt agccgtattt ttgacatttc
    32641 aagcagtatg ttttagcttc ctgatttttt caggattttt agataaactt ttttttagct
    32701 aacgaatagt gacatattaa tttatataac aaaggagaaa aggaaaatgg aagaacaaat
    32761 tttatcaatc ttagaagaaa taacaggaac agatgaagta agaaaaaatc gtgatattga
    32821 tctatttgat gaagggttgc ttgattcttt agcatctgtt caattattag tcgagctaga
    32881 cggagagctg gatatccaag tccctgtatc tgaattcgag cgagaggatt ggtgcacacc
    32941 gaataaaatc attcaacaag caactgcatt gaagggttga gtatgaaaaa gaaacttttt
    33001 gctattttcg gcccggttgt tttagctgtt ggcttactgt ttgtgttttt tacctcctcc
    33061 tatcaagtag atatctctaa tccagaaacg attagacgtg cctcttcttc catgtcagtg
    33121 aacgtgttga aaggagatgc gataaaaaat tatgcattat ctgaaaaaca atatattcct
    33181 ttttttggct caagtgaatt aagcagaatc agtccatttc atccgtctgt tctggcagaa
    33241 aaataccagc gaaactatcg tccatttttg ctgggagcac ccgggacaca atcgttaagc
    33301 caatatatga tgatgcgttc tgcaggagat gctatgaaaa acaagaaagt cgtctttatt
    33361 atttctcctc agtggttcgt taaaaatggt gtaaaaactg attactttaa tacgtattat
    33421 tctgaacttc aaacatacga ctggttgttt tcaatgaaga aagtaacgcc tgctgatcgc
    33481 tatttagcta gaagattatt aactttctca aaagtaaaag agaatgatac gttgacggcg
    33541 attctgcaaa cgatcaaaaa agggaagcta cctttacctg aatctttgaa tcagctgcgc
    33601 agccaatgga acatgctgaa gagagaggat gaagtattca gcaatattgg gttgaaagat
    33661 cgacagcaaa aaatcgatca tgagtcaaaa cgattaccaa aacaatacca agaaacagaa
    33721 ttatccattt tagcaaatca aattggagaa cgagaaacga cgaataatcc attcgggttg
    33781 aaaaacgatt tttatacgca tcgtatccga gcccacgagc cagagctcaa acagtcacaa
    33841 aaaaattggg attatcgttt ttcgccagaa ttttctgatt ttcagctggt cttggatcaa
    33901 ttagcaaaaa accataacga agtattgttc atcatcccac cggttaacga gaaatggagc
    33961 gattataccg gactatctca agaaatgtta caaggatttg ccaaaaagat caaatttcaa
    34021 ttaaatagcc aaggattcaa tcgaatagct gattttgtca atcaagcggg taccaactat
    34081 tttatggaag acacgatcca tctaggctgg aaaggctggt tggctgcaga tcaacagatc
    34141 agaccgtttt tagaagaaaa tcatattact gctagtaagt atcatcttga cgatgctttt
    34201 tttagcaaat cttggcaaca tcaaatacca gataaactac aattaaaata aagaaaacag
    34261 gcgagtgttg ctcgcttgtt ttctttattt ttgaatattc tgaataaaag ataaaaatac
    34321 caagtttgcc tgaacaaaaa tcataagcaa tggtataatc acaatatgaa tatagaaatg
    34381 ggggaaaaca tttggataca aaagaagaac agctacttat tgatttaact tctgcaaaag
    34441 gagtacctgg aaatgaagaa gaggtaagag aagtattcag agaatatgcc aaaccattcg
    34501 ctgatgatat tttttatgac ggtttaggca gtgtgattgc caaacatggt gctggcggag
    34561 gccccaaggt ctttatttct ggacatatgg acgaagttgg ttttatggta acaaaaataa
    34621 ccgaaaaagg attcttggaa ttccaaactc ttggaggttg gtgggggcaa gtcatgcttg
    34681 ctcagcaagt tgaaatcaaa acacgtgaag ggaaaatcgt ccacggtgtg attggttcca
    34741 aaccaccaca tgttttaaca cctcaagtaa gaaataaacc ttatgaaata aaggatatgt
    34801 tcattgatat tggcgcatca agccaagaag aagccaaaga gtggggcata cgtcctggag
    34861 atatggtcac tccatatatt gaatacaaac gaatgaatgg cagtaaatat ttactggcaa
    34921 aagcatggga taatcgtatc gggacagctg tttctctgcg agtattagag aatctatcta
    34981 aagaagcaca cccaaatgtt ttattcgcag gaagcgatgt gcaagaagaa gtaggcttgc
    35041 gaggtgctcg aacaagtaca caccttgtaa atcccgatat tgcttttgct ttagatactg
    35101 gtacagcagg agacactcca ggaatgacac caaaagaagc agattcgatt ttaggaaaag
    35161 gcccacagat tttgattttt gatgcatcta tgatcccgca taaaaaatta ttgaattttg
    35221 ttattgatgt agcagaggaa atggatattc cattccaata tacagtgatt tctggcggag
    35281 gaactgatgc aggacaaatg catttgacaa gagatggtgt tccgtctgta gccattaccg
    35341 ttcctgttcg ttatctgcat tcccatacat cagtgattca tgaagatgat tatttgaata
    35401 cagtgaaact agtcacagaa ttagtgaaac ggcttgatag tgaaacagtt gaaaaaattc
    35461 ggagttatga ataatgaata atagaatacc tatttctgaa tatcggaaaa aagcacgagt
    35521 ttctttggaa aatcaatggg gaatcaatac ttggcttgtt ttcttatcta taatatttac
    35581 gagcgtcatc cagaactttt ttgacggttt cagtcctttt ccggaagaaa gcatgcagca
    35641 gcacttattg agttttctag tcgataattt acttttattc gcattcaact atgcattgtt
    35701 ttacattgcg ctagtagttg tacggggtgg cagaccaaaa agtaaccttt tattttcagt
    35761 tttccaaaga aagtattttg gttcgatact tggcatcaat ttgatcaatt cagtcatcaa
    35821 ctggctactc aacagtttaa tctttctgcc catgttttta atcagcggag tgagtactta
    35881 tatgcagctg cttttcagtt caaatgcggt acctacgggt gtcatgccag aagattttct
    35941 tgatatcagc tttgttttat cgatgatagg gatgattttt gtttcagcac tcatcatgag
    36001 tattgttggc ggtttattcc aatttgctgc atggacaaag ttcgactatc ctgatttgtc
    36061 ggttattcaa tgtttgaaat atggctggtt tttattgaaa gatagaattg gtacgtatat
    36121 tttgttgcag ctgtctttta tcggctggta tttgctagga gcaatcgctt tgttcatcgg
    36181 tcttttgtgg gtcattgttt atgtgaatgt gtcagttgca gaattttatg aacaagcacg
    36241 gattgaaaaa ggaggtccag acgcctattt tacttagcat gtaagcagtt tattcatcaa
    36301 aaaaataact tttgtggtag gataaatctt tgagggggaa tgtacgatga aattaactgt
    36361 tttaggatgc ttaggagcgt atccttataa aaatcaaggg acaacaggtt atttgcttca
    36421 atctgacggg ttcaatttgt tgatcgatgc aggtagtgct acgctgatca aattacaaga
    36481 acaccttgat ccacttgatc tagatgctgt gatccttagc cattatcatc atgatcatat
    36541 tgctgacctt ggtgtattac agtattactg gcaacttcat cctaagagaa atccggagac
    36601 attgctttca atttatggac acacaaaaga ttccattcac tttgaagaat tgacgttgga
    36661 aaatgtctca aaaggacatc cttattttga agctgaagaa ttaaaaattg gtccttttct
    36721 gattactttt atggaaacaa tccatccagt agtatgttac gccatgcgta tcgtagaaga
    36781 aacaacgggg aaagtattcg tatttacagg tgattcaggt tatttggaaa gtttcgctga
    36841 ttttgcgaaa gatgctgatt tgtttctagc agatacctat ctttttgaag gaaatgaaca
    36901 tcatcatgcc catttcactt caaaagaagc tggagaactt gcgaaatcag ctaacgtaaa
    36961 gaaacttgta cttacacacc tacctcagtt tggtgattta gaacaattga aaaatgaagc
    37021 gcaaaaggca gcaggcaaag ggattgatat agaacttgct gcagttgaca aagtgtttaa
    37081 catttaggag gaagaacatg atttttgtac caaatgaaaa ccacgaccca agaatcaatt
    37141 tagcaattga acaatatttg ctgcaagaaa tgcctttaga tgaacctatt ttgctctttt
    37201 atatcaatga accttcaatc atcatcggca gaaaccaaaa tacgcttgaa gaaatcaatc
    37261 gagattatgt ggaagaaaat ggcattcatg ttgtccgtcg cttaagtgga ggcggagctg
    37321 tctaccatga ttttggaaac ctgaacttta gttttatcat gcctgatgat ggggattcat
    37381 tccgtgactt tgcgaaagta acacagccaa tcatccaagc acttcatgaa ttgggtgttg
    37441 ctggggcgga attgaaaggc cgaaatgatc tagtcattga tggcatgaaa ttttctggca
    37501 acgcgatgta tgctaccaac ggtcggatgt ttgctcatgg aacaattatg ttcgactctg
    37561 atatcaatga agtagtcaat gcattaaaag taaaaaaaga caaaatcgaa tcaaaaggaa
    37621 tcaaatcgat ccgttctcgt gtaacgaata tcaaaccatt tcttccagaa gaaaagcaaa
    37681 aaatgacaac agaagaattt cgtcaagaaa tcttgttgaa aattttcggc gtagagtcag
    37741 tagaagaagt aaaaacgtac gaattgactg atgaagattg gaaaaaaatc aatcgaatct
    37801 ctgaggaata ttatcgaaat tgggattgga attacgggaa gtctccagat ttcaatttct
    37861 ctcgccaaaa acgtttttct atcggttcga ttgaagtcca tttgaatgtc tcagaaggaa
    37921 tgatcaaaga agcaaaaatc tttggcgatt tcttcggact aggagatatc tctgacgtgg
    37981 aaaaaaaatt ggttggacag aaatatgata aagcatctct atcaaaaata gtcagccaaa
    38041 ttgatgtgaa gaaatatttt ggtgcgatag aaccagaaga attgcttacc ttactttatt
    38101 gaaaataaaa aatagcggaa tccacatata tggattccgc tattttttat acaggattcg
    38161 ggtaatttcg agagagttga acagtgatcc gatcaatgaa agtatctttt tcctgcgtta
    38221 gtgttcctgt gtaacgatcc gctacgtctt tcatcatcat taagtcaaaa cgttcctgat
    38281 catctaattc ttctgtgatg cctcgactaa ataggtttgt atcgtaatgt gcactatgtt
    38341 cgcaacaaat gacgaaatgt tcctcttgtt cgtgtatcga caaataaatt tttcgataca
    38401 gtggattttc gatggcatga gtttctctaa aggaatgctc taaagcgtgg atcaaaagtt
    38461 gagccagatc gtcatcgttg ataggaagtt cttcgggaag atctgcttgg aaggttatct
    38521 gaatattccg tttggcagct aacttctggt aacgcgctaa gataagattt gtcacctggt
    38581 ggttgcaaaa agaacacagc atttcgaaat tttttgcttc ttctaatatc ttttccacat
    38641 agtttttagc ttttgtttgg tccgtatgca ttaaatcttg cagattctcc atctcttgga
    38701 caaattccag gcgaagtgta tttacttctt ggatatgctg tcgaagctgc tcataatgtt
    38761 gctccaacaa atcatttttg atttttaaga aatctacttg tctccgatct ttgtcggtat
    38821 ttaagatgtg atcgattaga ttgtagctga taataacgat tagaatgata atatataaaa
    38881 tcgtagacca attgatcgtt gtttgataga tacctaatgc cgtttggatg taaatcaagc
    38941 aatggataat ggctaccaag acgatccaag gtgtacaagc tacataaaaa cggttattct
    39001 tatatgcttc gccaatgcat gcaagtgaag tgactaaagt gcttataaca ctagcaatat
    39061 tagcaatcgg cattaattca ggaagttctg caggagtaaa agttgcataa agcagaacgc
    39121 cgataaaaat caaaagttgg aacgtacaaa aaattccgta ccatttttta tagtaaaaca
    39181 ttcggctcca atagtagcag atgatgaaag ctgacgttaa gataataaat aaatgagata
    39241 aaaaagaatg aacgaatggt tcaaaaagaa taccacttaa aatatcttct gatacagctt
    39301 caaatcctaa tgccaaagca aagcaactta agagaataag tgagtattca agacgttttt
    39361 gtttatacat caagtagatc gtatagaaaa aggtccctat agcgataaaa acagcgatta
    39421 tcatcgtcaa tacttgcggc actgattgtg aataaataaa actaatcaat ggtccagttg
    39481 ttcctagata aactttcggt ggcagtccag catagttctg ataaggagat gagactgtaa
    39541 gggtcaattt ttttccgata taatttgctg gtaaatctat cacagccaat gagagtccgg
    39601 gattgctttc ttcctgataa tctttccgtt ggtataaaat cttttcgcct agatatacag
    39661 taatccattg atggtttcct ttgattagta aatttgcttc gtccaacggt ttttcaagtg
    39721 tcgtttccat aatgaatgtt tcgtctttat ccacggtagg aatcgtacga gtgtacttgc
    39781 tgtcaaactt aaaatcagga tcatcttttg tatagaaaac ccacgaatct tttatttctt
    39841 tcatttgatt gctgtccgca ggagaaaaaa taaaataaaa gaaataagca attcctgtta
    39901 aaaataacaa tgataatata atgattatgc tccactttat acgtaaataa aaagattctt
    39961 ttttattata tatgttatta aaaaaagaca taaaggtatt cctttctaaa acttgta
//
LOCUS       DAYYFD010000014        39528 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000014 DAYYFD010000000
VERSION     DAYYFD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.014"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..935
                     /locus_tag="ACHA25_000686"
     CDS             78..935
                     /locus_tag="ACHA25_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8762013.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1050..1970
                     /gene="pstC"
                     /locus_tag="ACHA25_000687"
     CDS             1050..1970
                     /gene="pstC"
                     /locus_tag="ACHA25_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8762014.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1970..2854
                     /gene="pstA"
                     /locus_tag="ACHA25_000688"
     CDS             1970..2854
                     /gene="pstA"
                     /locus_tag="ACHA25_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8762015.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2869..3675
                     /gene="pstB"
                     /locus_tag="ACHA25_000689"
     CDS             2869..3675
                     /gene="pstB"
                     /locus_tag="ACHA25_000689"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8762016.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3691..4449
                     /gene="pstB"
                     /locus_tag="ACHA25_000690"
     CDS             3691..4449
                     /gene="pstB"
                     /locus_tag="ACHA25_000690"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8762017.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4462..5139
                     /gene="phoU"
                     /locus_tag="ACHA25_000691"
     CDS             4462..5139
                     /gene="phoU"
                     /locus_tag="ACHA25_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8762018.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5716..6408
                     /locus_tag="ACHA25_000692"
     CDS             5716..6408
                     /locus_tag="ACHA25_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8762019.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6405..7172
                     /locus_tag="ACHA25_000693"
     CDS             6405..7172
                     /locus_tag="ACHA25_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8762020.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7272..8840
                     /gene="liaX"
                     /locus_tag="ACHA25_000694"
     CDS             7272..8840
                     /gene="liaX"
                     /locus_tag="ACHA25_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8762021.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9137..9457
                     /locus_tag="ACHA25_000695"
     CDS             9137..9457
                     /locus_tag="ACHA25_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8762022.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9457..9807
                     /locus_tag="ACHA25_000696"
     CDS             9457..9807
                     /locus_tag="ACHA25_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8762023.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9974..10912
                     /gene="hprK"
                     /locus_tag="ACHA25_000697"
     CDS             9974..10912
                     /gene="hprK"
                     /locus_tag="ACHA25_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8762024.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10927..11757
                     /gene="lgt"
                     /locus_tag="ACHA25_000698"
     CDS             10927..11757
                     /gene="lgt"
                     /locus_tag="ACHA25_000698"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8762025.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11801..12826
                     /locus_tag="ACHA25_000699"
     CDS             11801..12826
                     /locus_tag="ACHA25_000699"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8762026.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12841..13779
                     /gene="galU"
                     /locus_tag="ACHA25_000700"
     CDS             12841..13779
                     /gene="galU"
                     /locus_tag="ACHA25_000700"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8762027.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13904..14344
                     /locus_tag="ACHA25_000701"
     CDS             13904..14344
                     /locus_tag="ACHA25_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8762028.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14347..14949
                     /locus_tag="ACHA25_000702"
     CDS             14347..14949
                     /locus_tag="ACHA25_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8762029.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15008..16114)
                     /locus_tag="ACHA25_000703"
     CDS             complement(15008..16114)
                     /locus_tag="ACHA25_000703"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8762030.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16324..17325
                     /gene="ccpA"
                     /locus_tag="ACHA25_000704"
     CDS             16324..17325
                     /gene="ccpA"
                     /locus_tag="ACHA25_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8762031.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17662..20007)
                     /locus_tag="ACHA25_000705"
     CDS             complement(17662..20007)
                     /locus_tag="ACHA25_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8762032.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20286..21188
                     /locus_tag="ACHA25_000706"
     CDS             20286..21188
                     /locus_tag="ACHA25_000706"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8762033.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21401..23176
                     /locus_tag="ACHA25_000707"
     CDS             21401..23176
                     /locus_tag="ACHA25_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8762034.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23242..24828)
                     /locus_tag="ACHA25_000708"
     CDS             complement(23242..24828)
                     /locus_tag="ACHA25_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8762035.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            25079..25303
                     /locus_tag="ACHA25_000709"
     CDS             25079..25303
                     /locus_tag="ACHA25_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8762036.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25444..27195
                     /locus_tag="ACHA25_000710"
     CDS             25444..27195
                     /locus_tag="ACHA25_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8762037.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27195..28979
                     /locus_tag="ACHA25_000711"
     CDS             27195..28979
                     /locus_tag="ACHA25_000711"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762038.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29305..30156
                     /gene="licT"
                     /locus_tag="ACHA25_000712"
     CDS             29305..30156
                     /gene="licT"
                     /locus_tag="ACHA25_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8762039.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30252..32132
                     /locus_tag="ACHA25_000713"
     CDS             30252..32132
                     /locus_tag="ACHA25_000713"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8762040.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(32172..33074)
                     /locus_tag="ACHA25_000714"
     CDS             complement(32172..33074)
                     /locus_tag="ACHA25_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8762041.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            33305..34708
                     /locus_tag="ACHA25_000715"
     CDS             33305..34708
                     /locus_tag="ACHA25_000715"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8762042.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            34708..36141
                     /locus_tag="ACHA25_000716"
     CDS             34708..36141
                     /locus_tag="ACHA25_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8762043.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            36286..37212
                     /gene="trxB"
                     /locus_tag="ACHA25_000717"
     CDS             36286..37212
                     /gene="trxB"
                     /locus_tag="ACHA25_000717"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8762044.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            37426..39153
                     /locus_tag="ACHA25_000718"
     CDS             37426..39153
                     /locus_tag="ACHA25_000718"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8762045.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            39168..39518
                     /locus_tag="ACHA25_000719"
     CDS             39168..39518
                     /locus_tag="ACHA25_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8762046.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        12644 a         6973 c         8511 g        11400 t
ORIGIN      
        1 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
       61 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcta
      121 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
      181 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      241 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      301 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      361 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      421 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      481 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      541 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      601 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      661 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      721 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      781 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      841 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      901 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
      961 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
     1021 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
     1081 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1141 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1201 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1261 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1321 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1381 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1441 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1501 gaagtatttt tggcgggact gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1561 tgatcttgcc gactatcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1621 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1681 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1741 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggtctta
     1801 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1861 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1921 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     1981 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     2041 tttagcagct ttactacttt atatcttagt tcggggaata ccgcacattt catggtcctt
     2101 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2161 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2221 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2281 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2341 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2401 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2461 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2521 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2581 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2641 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2701 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2761 cgcttctgcc gtgttgatta ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2821 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2881 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     2941 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     3001 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     3061 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3121 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3181 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3241 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3301 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3361 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3421 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3481 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3541 caagcagctc gtatcagcga ctatactgcc tttttctatt taggaaaagt aatggaatat
     3601 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3661 ggtcattttg gttaagacgg aggcctcaac atgacaaaag agattatttc atctaaagat
     3721 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa
     3781 ggggaaatca ctgcgatgat cggtccttct ggttgcggaa aatccactta tctgcgttct
     3841 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3901 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     3961 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     4021 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     4081 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4141 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4201 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4261 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4321 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaatgat
     4381 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4441 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4501 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4561 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4621 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4681 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4741 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4801 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4861 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4921 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     4981 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     5041 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     5101 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5161 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5221 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5281 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5341 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5401 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc cgctttatat
     5461 gaagtacctg tcatcgagat agaaaaagta cgaataaaag aggatcatca attgattaca
     5521 ggaaaactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5581 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5641 ggagatgaat ggcagctagt agcaaaaaaa aatgatggct ggcttttctt cttcacaagt
     5701 atcttcattg gcgccatgtt gcttattggt ggaaaacagg gtctatttgc tttaggaagt
     5761 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
     5821 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
     5881 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
     5941 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
     6001 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
     6061 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
     6121 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
     6181 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
     6241 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
     6301 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
     6361 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
     6421 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
     6481 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
     6541 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
     6601 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
     6661 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcaatc caaggtttta
     6721 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
     6781 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gcgagtgtag
     6841 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
     6901 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
     6961 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
     7021 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
     7081 ccacattgtc cgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
     7141 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
     7201 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
     7261 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
     7321 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
     7381 tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
     7441 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
     7501 gagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
     7561 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
     7621 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
     7681 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
     7741 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
     7801 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
     7861 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
     7921 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
     7981 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
     8041 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
     8101 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
     8161 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
     8221 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
     8281 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
     8341 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
     8401 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
     8461 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
     8521 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
     8581 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
     8641 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
     8701 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
     8761 gtgtttcaga tggtgaaatc gcgcaagtgc aactttcaac gacaacagga agtatctatt
     8821 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
     8881 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgatagtcta
     8941 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
     9001 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
     9061 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
     9121 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tctaataaaa atgttgtttt
     9181 gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt
     9241 gctttatgta ttcctgagct tagttgcttt tggaagtcca attattttat acattttatt
     9301 agctttgatt atcccatttg cgcgttctgc acgaacaaac tatggacata acaatgcata
     9361 ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc
     9421 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta
     9481 gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc
     9541 agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa
     9601 ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt
     9661 gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt
     9721 ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt
     9781 gtcagcgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt
     9841 tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt
     9901 ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa
     9961 tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt
    10021 ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc
    10081 acgcccagga ctagagttga ctggttattt taattattat tcccataatc gtttacagct
    10141 atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat
    10201 catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccagc
    10261 tccagaagaa atgatccagg cagcaaataa aaatgggata gctgtactgc gttctcccat
    10321 ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg
    10381 aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga
    10441 cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggcc atcgattgat
    10501 cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc
    10561 taaaatcctg gaacatctga ttgaaatccg aggaatcggg atcattgatg taatgaatct
    10621 ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc
    10681 atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc
    10741 ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat
    10801 cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac
    10861 atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt
    10921 gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac
    10981 tggtatgcat tgatcatcgt atcaggtatc attatcgcta tgtggcttag cagcagagag
    11041 gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct
    11101 atctccatta ttggcgctcg cttatattat gttttatttg atcttcccca gtatatagct
    11161 gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt
    11221 gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt
    11281 ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc
    11341 atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta
    11401 ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt
    11461 tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca
    11521 caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt
    11581 ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct
    11641 caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa
    11701 aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt
    11761 ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt
    11821 tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga
    11881 cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa
    11941 tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga
    12001 agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc
    12061 tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa
    12121 aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc
    12181 agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcaaa
    12241 acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca
    12301 agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga
    12361 gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg
    12421 gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt
    12481 aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggaa taggcgatct
    12541 agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca
    12601 agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac
    12661 tacccaagca gcgatggaac tagcccaaat gatggaaatc gaaatgccca tcactcaaac
    12721 aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt
    12781 acgtgatgga aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat
    12841 atgaaagtta aaaaagcagt tattccagca gcaggattag gaacaagatt tttaccagca
    12901 acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc
    12961 gttgaagaag cgttgaaatc aggtatcgaa gacatcttga tcgttacagg aaaagctaaa
    13021 cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa
    13081 ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc
    13141 cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga
    13201 aatgaaccat ttgtcgttat gctaggcgat gatataatgg aagacgaagt accattgaca
    13261 cagcaattaa tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt
    13321 cctcatgatg aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg
    13381 ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta
    13441 gctattattg gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa
    13501 ccaggagccg gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa
    13561 cgcgtatttg cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt
    13621 atgaaaacaa gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag
    13681 tacatcctgt ctttaggtaa aacattagaa gcagaaggaa gcaagatcga tcataatcca
    13741 aaaggaataa cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc
    13801 agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa
    13861 atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat
    13921 tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt
    13981 gttgcaagtt tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac
    14041 gatcgaaatc gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttactggt
    14101 caaaagtaat gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt
    14161 cacagcagtt gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat
    14221 cgcgacgaaa gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt
    14281 cggtgggaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa
    14341 ataaaaatgg ctaaaaaagg tggattttta ttaggtgcag tagtcggagg cacagccgca
    14401 gcaatcgccg cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct
    14461 gcccaagcgg atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa
    14521 tcgactgagt taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt
    14581 gatctagcag gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa
    14641 tctgacgata tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa
    14701 aaaacggcag aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact
    14761 tcagaagata tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat
    14821 gaaggaatgt cagaagcaaa accagttgca gatgactcaa aagaaatgat gaaaatggca
    14881 aaagaagatg cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca
    14941 aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc tctcaccttt
    15001 tttactctta tttttctaaa acaagtaatt cttttggtgt agtcgtgaat ggcacacagc
    15061 catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca ggaatataga
    15121 ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt ccttctacaa
    15181 gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga ttgaaatatt
    15241 cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca cctgctgtca
    15301 cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg ttgtagattt
    15361 tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca ctgcaatagc
    15421 cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc ttcgtatttc
    15481 ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg tcgaaagaca
    15541 tttgtgaaac accttgtttt ttcaattggt attcaatttc tgcaatgatc gcttgttcag
    15601 tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc catttaccag
    15661 cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt tggatgatgg
    15721 aagttagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca tcgaagcgct
    15781 cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag cgagcacgaa
    15841 gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg acatcatacg
    15901 cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg aaaggatctt
    15961 gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg tatccggaaa
    16021 agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt tgtgccatcc
    16081 attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc taaattaatt
    16141 ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa aactgaaaaa
    16201 agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat tatttgatat
    16261 tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa taggagaaca
    16321 caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa tgtttcgatg
    16381 gctactgttt cacgtgtcgt aaatggcaat ccaaacgtta aaccagcaac aagaaaaaaa
    16441 gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg tggcttggca
    16501 agtaagaaaa caacaacagt tggtgtaatc atccctgatg tcagcaatat gttctttgct
    16561 tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat tatcttagca
    16621 aattcagatg gcaacgacca aaaagaagtc aatgtattga ataacttact ggctaagcaa
    16681 gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg agaattttca
    16741 cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca agtaggtagc
    16801 gtaaacattg attatgtatc cgcaacaaaa gatgcagtaa acaaattggc aaaaagcggc
    16861 aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ccatcaatgg acaaaatcgt
    16921 ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga aggattgatc
    16981 tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat ccataatagc
    17041 ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt ggatggttta
    17101 ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa caattcacta
    17161 ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata cgatttaggg
    17221 gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga agaaaaaacg
    17281 attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa ggacaaccat
    17341 tcagaggttg tgtgcctgcc gtttaactct gcgttacaag gagcaatttc aaaaaacagc
    17401 atctcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga acaccgttta
    17461 tttgggaaaa ggggttatga caaaattttg ttataatcct ttttttgtgt cttaaaaaag
    17521 agagaaagtg gttgaaatta catttatcgt atataaaaca atccgtgtat agatgctgca
    17581 ttttgttcac caccatcaaa aaaataaggg actgtgacaa tactattgtc acaatccctt
    17641 attactgtgt attctattaa atcaatcatc tttctcatct gattctttag ttgcttgatt
    17701 cgctcttgca taagctgaag cggtgttcca gtaatctgta tagttttcat cggtaccgcc
    17761 aacgccaaat ttaaaggcgc ttttcttcgg tccatttttc gcccatagcg attcaactgt
    17821 ttttcctggt gtttggatgg attttttatt gacagtaact tttccaggtg tttgaccagt
    17881 aaatccagat acttttttct tcatcacatc agatgataat tcgaatttct catcagtgcc
    17941 aaaaatgtca ggttttgctt ggtaagcttg attgatcaga ttggcaagat aattagccga
    18001 acgtatcctt gctttgctgt ccattccagt attgtcatcg tgccctgccc agctactgat
    18061 ggtaatagac ggagtagaga caattagcca tgaatctcgg tattcattgg tagaaccagt
    18121 ttttccgacc caatctgctt ttcctagatt tccgtttaag cttgagatgg catctttgaa
    18181 tggtgtagtg atttttgcat tgatcacact acgcatcatg tcattcatga tcgatgccgt
    18241 tgcttccgaa tagatccgga cagagttgct ttcgtgttta taaattacat ttcctgcgtt
    18301 atctgtgatg gaatcgatga tatagccttc ttcatagacg ccgccgtttg ctaaagcctg
    18361 aaatccgttg gtctgttgaa gcgtcgtgac atctactgta cctaaaggag cagattcaac
    18421 gccccaattg tcatttgccg ggtagttcat ctttgccaag tagtgctcgt aagcatattg
    18481 tttcgatcca ccggaattca acacatcttg atataagtga taagccggga tattgttgga
    18541 ccattctagt gactcacgga cggtttggaa ggtgttcgat ccttcgttag tagcattgac
    18601 gattttctcg ccagcatttt ctccttcttg ccaagttgtt gcataatctg atacacggga
    18661 ttctgatccg ataagcccca tgtcgattgc aggtccataa acaagaactg gtttgattga
    18721 tgagccagct tgacgttctg tatcgaaggc atggttattt tgattctgac tgtaattccg
    18781 tccgccaacg aatccgtaga tgcggccagt tctgttatcc atcaatacat ttccggtttc
    18841 tacttgactg ccagcaccat catctaacat gtatccataa ttttcaacgc cattttgcaa
    18901 tgcagcatag acatctttat cgattgtcga atggattgta tatcctttat tttggatcgt
    18961 ttgctgtgcc ttttgataat aagcgtcact gaccgatgaa tcagacatat cagcgttgtc
    19021 tttttcagcc aattgttgtg caatgattct tgttgcttca ttcatcacag tataatacaa
    19081 gaattctctg tcagtctgtt ctgcgatttg ttgtggtaga aagtcttttg taaggtcata
    19141 ggcttttgct tcttcgtatt gttctttcgt aatttggcct tcacgataca tactgaataa
    19201 aacaaaatct tttctggcaa ggccggcaga aagatcttct tttagcgcgc ctgtgttcgt
    19261 gtatggacta tacgtgatcg ggctttgggg taatccagcg atataagcag cttgcggcag
    19321 cgtcacatct tttgcatcta caccaaagat tccttttgca gcttcctgga ctccagcaat
    19381 gttttgtccc ttattattac gaccaaatgg tgagacattc aaatacgtag caatgatctc
    19441 atcttttgaa aagtattttt ctacttgtgc agaaagaatg atctcgtttg ctttacgttt
    19501 gaatgttgtt tcatctgtaa ggatctgctg tttgactaat tgctgtgtca aagttgatcc
    19561 accagaagag ccaataccag tagcttcaga tactaatgct cgtaaaacag ctttaggaac
    19621 gtaaccttgg tgtttatcga agtattcatc ctctgtgctg ataattgcag ttttcaataa
    19681 aggtgaaatc ttatcagatg aaatagtcgt gcgcatcaga tctgtctgga tttttgatat
    19741 ctctgtatta tctgcataaa caatctttga cgtttctgta atgtttccta gttctgtctg
    19801 cagtgctttt ttagtcggca attgtgtgtc ttcgacgaga taggcaaaat agccagcgcc
    19861 tattccaata cctagagctg cgaacatcaa aagaagaaca gtaataaaaa cagtcaatga
    19921 ttgaaaaact ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg
    19981 tttttttcga gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct
    20041 aattatagca aataatagaa caaaaaaaca gacaaggctg tttttggcag gaatttcttt
    20101 tttaaaagag ttaaaacgtg agaaaatgat ctttattata aaagagttct taagcttttc
    20161 tttagagaaa atttcagtta ctatggagaa cagggcaaag aaaaatcaat cattttcttt
    20221 gtatatcatc ttaccaatat ataggaattg ttagtaacaa ttgcctttca aggaggaatc
    20281 catccatgtt attaggctca cacgtcagca tgagcggaaa gaaaatgctg ttaggagcag
    20341 cagcagaagc agccagttat ggatcttcga cttttatgat ctatacaggc gcccctcaaa
    20401 acactagaag aaaagccatc gaagagatga atattgatgc cggaaaagcg tacatggcag
    20461 aaaacggtct aacaaatatc gtcgttcatg ccccttatat cattaatttg gggaatacga
    20521 tcaaaccaga aatgtttcct tttgccgtcc aatttctgag agaagagatt gttcgtgcgg
    20581 aagctttagg cgctactcaa attacacttc acccaggtgc tcatgtaggg gcaggaacag
    20641 aagccggaat tgctagaatt attgaaggct tgaatgaagt tttgacaaaa gaacagacgg
    20701 cacaaatcgc tttggagaca atggcaggta aaggtacgga aatcggtcga acatttgagg
    20761 aattagcgca aatcatcgat ggagtaaaat taaatgaaaa attatccgtt acttttgaca
    20821 cctgtcatac gaatgatgcc ggttataata ttaaagaaga cttttcaggt gtaatggaag
    20881 agtttgataa gatcatcggg ttagatcggc ttaaagtact ccatatcaat gattctaaaa
    20941 atccgcaagg ttcgcataaa gaccggcacg ccaatattgg ttttggaaca atcggattcg
    21001 acacgctgaa taagatcgtc catgatccaa tgtttgacaa tatttcgaaa atcttggaaa
    21061 ctccctatgt cggcaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatcgcca
    21121 tgttcaaagc acaaatattc gatccagaag tgtttcctga gttattagca gaagaagtga
    21181 tactctaaga ttttctatct gacagcagca aaaaaggacc aggcacattg ccttgtcctt
    21241 ttttgtttaa gaaacaaagg agtacttttt gcagtctttt tcatcatgca tcttctcccc
    21301 cctcttttta aatttagaca aacaaggtat actgttagaa aagtaaaaaa acgaaaagtt
    21361 tcacaagcaa gtggcaaaac gtgattggaa ggacgaaaag atgctgaaca ctttaaaacc
    21421 cttgatcaac caactaaaaa agaaacatac atactttatt cttggcatag ccctattatt
    21481 tacaattatt cgtttgtatt tatatagtaa agcaatctat ggaacagaca tgtcggcaac
    21541 ctatgatgac cagctgctga ttcgctatgc tcaaaatatt ttggacggta aatggctagg
    21601 ggagtatacg actacaacac tttccaaagg aatctcctac agcttatttt tagtacttgc
    21661 acataaactg catctttctt atgctgtttt gttcggcatg ttcagtattt ttgccagtat
    21721 attggttgca ctcgcgttta aaccgatcgt gaagaacaag tatcttcttt taagtatcta
    21781 tttttatttt ttgttttctc cgattagttt tacacatgaa tattccactc gaacatatcg
    21841 taatgccctc gtgattccgg ctgttgagat cattatcgct tgtttactag ctatttatta
    21901 ccgcaaatac ggttctagca gaaaattgct gccatggttt ttagggcttt ctgtcgcttt
    21961 tcctttcttt tggttcattc gcgaagattc tatctggctt cttcctttta ctgtagtagc
    22021 tttgggtatc agtttcgtcc agatcttgct agggcaagac ttttctttca aagaagtaaa
    22081 gaagttaaaa aaacgactct ttcttctctc gaaacgggaa tggctgaaac tttcattgtt
    22141 tttacttcct atagttggct tttccatcgt ctatcagtgg atcgaatata aaaatgagac
    22201 aacatacggt attgcaacca tcaatgaccg ttccagtgga gaatttggac gtttgatgaa
    22261 acaactgatc cgaatagatg atgaaacgaa cttgaataaa gaaaacagtg agatatgggt
    22321 ctcacatgct gcactaaata aagcattagc cgcatctcct acattccata ctgtttctac
    22381 ccgtattgac gaattatata cgactcacgc gtggactcaa ggtggaaaag taaaagaatt
    22441 acccggagat atcatctttt gggcattgcg agatgtggta agcgaatctg gttattacca
    22501 aaacaatgca aaagaaacca atgatttttg gaaaaaagtc aaccaagaat tagaagaagc
    22561 ctatcacaag ggaaccatca aaaagaaaaa agagttctat ctgatgggat caggagatgg
    22621 aaagacgatg agtgatctgc caatcgtatt cgatttcctt caatcaggat acgcttatgc
    22681 atggcgatat aaagatttcc atcaaggagg aaattatagt ttcggaacgc aagaacaagt
    22741 caaacaggct caagaattat taaaccttcc gctaatggag aattggatcg atagtaatga
    22801 accgagaaca cctaatttcc aattaacaaa atcagcaaaa gtcgcgaatg tcatcataaa
    22861 aatttaccaa gttctggcta ttcccgtgct gattgcttca atagcaggga tattacttat
    22921 gatactagga accgtatttg caaaagctga tcgtgcctat tatagttcct ctttgattgt
    22981 ggtcaccgga ttaatactga cacaattggt ttttcttttc ggtgtctcgt ggttttgttc
    23041 ttatgcgcca gaaaaaaagg attatttttt aagtgtctac actggcgcag gcgtgccatt
    23101 aacgcaattc atcgtagtaa caagcatgct agttttcagt aaattaaatc tgaaaaaggc
    23161 tttatcttct aaataaaaag acccttggta gctagatttt taacctaact atcaagggtc
    23221 tttgtgtgga aaaatcagtt tctattcgat ttcttctacc gtttcctgca aacgctgttc
    23281 atattttttg tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag
    23341 aacaatgatt acataagcga tgtgatcagc caatgctagt tgagcagaag tggcttgttc
    23401 accaaagaaa ctttcgatct gtcctggtaa tcctcgtaag ttcaagtaag ttagactaat
    23461 cacagagaac cagccaagaa gttttacgat gaatgaattt ttaaatcggc tacccatctc
    23521 cgcttggctg ttggtcatca tcagtaaagg gatcatcgaa aacggcaacg caaaagccaa
    23581 gaacacctgg gattcattca tcaagttatt caaagcggta tgttcttcga tcgttccttt
    23641 tgtacttgta aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgaccag
    23701 tcttcttagc caaatcggga tacgcatatg gatgaagcct tccatgatga cttgaccggt
    23761 caacgtacca gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt
    23821 ggataagata ccagattttg ctacagcaat caagatacca ttgctcatag ctgatggatc
    23881 agataaggct tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaagac
    23941 agcgacaccc atgatcaata gcagtgcatt gactagaaaa gccatcgaca attggatatt
    24001 agagtcccaa gtagaaaaac gtacagcttg tgctacatct tcttcgtctt ggtgatcaat
    24061 cttccgagtt tgagaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc
    24121 aataatccct aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa
    24181 tgtatgactg ttagggataa agccagcaaa taaactgtgc caatctgggt tagacaagcc
    24241 aacttgatag acaaaaacga ataaaatgac gaaaattaag cagacaacca gtgcttcgat
    24301 cttacgaaac ccgacttttg tcagtagtaa gagaacgaaa acatctaata cagtaataaa
    24361 gacggcaatc accaatggaa tatcaaaaag taagtagagg gcgattgctg caccaatgac
    24421 ttctgcgatg tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacccaa
    24481 tgtcttactt gtgcgtgctc ggattgcttg ggccaaatcc atttgggtca cgattcctaa
    24541 ttttgctgcc atatactgta aaagcatagc aatcagacta gacatcaaaa taactgacat
    24601 caataaatat tggaaatttt gtccaccagt gatcgaagtg gaccagttac ctggatccat
    24661 ataaccaact gctactaatg cacctggtcc agagtaagca agaagtgttt taaaaaaact
    24721 tttccccttt ggtacatcaa ctgttccatt tacttcagct aaagataaac catttgtatg
    24781 ttcaataagt cgatgcttat gaaattgttt gttggattga ttattcaaga aaaaaccacc
    24841 tttcctattt atctacgatt acattctaca cctcaaaaag aaaaaattaa agaaaaatat
    24901 tcggtatgcc taatttattt tcgtggcgcg tcttgttaga tgacgaaaga aattttgttc
    24961 tcctctaaaa aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa
    25021 taaactttag taaaataagt cattgagagc aattcggaaa tagaaaggag agatttatat
    25081 gttatcaaca ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg
    25141 tggctttttc ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa
    25201 tgaggacatg ttgcgatcaa tgatgatgtc aatgggacaa aaaccatctg agaagaaaat
    25261 tcgccagatg atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaacgact
    25321 ttttacgggc tgattttttc agtccgtttt ttattttgta atttgttaga ctattcagat
    25381 tattcagcaa aaagacaagc acagacaatc ctaggctctt gataaagaca aaggaggagt
    25441 aaaatgtcga tttttagaaa gttaggctgg ttttttcgtc aggaaaagaa acattattta
    25501 attggggttt tctcgctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt
    25561 gggatcatca ttgatgagat tgccgataaa aatttatcag taaagatcat tttcgggtgg
    25621 atcttcctat tgattatcgc tgctttctta caatatcttt ttcgttatat ctggcggacg
    25681 aatatttggg gaagtgcagc tcggttggag aaagaacttc ggcagcagtt attcaaacat
    25741 tttacaaaaa tggacaatct cttttaccaa aaatatcgga ctggggattt gatggctcat
    25801 gcaacgaatg atttgaatgc gatccagaat gtagcaggag ctggtatctt gacctttgca
    25861 gattcagtga ttacgggcgg aacaacgatc attgtgatga ttttgtttat tgattggcgc
    25921 ttaacactga ttgcgctgat tcctctgcct ttgctggccg tcacttcacg agtgctgggg
    25981 tcgaaactcc atgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag
    26041 acacaagaaa gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat
    26101 ctagcggatt ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt
    26161 ctagactcct tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca
    26221 attattttag gtggaaagta tgtcttggaa ggcacgatct cgatcgggca gatggtctca
    26281 tttattagtt atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac
    26341 gtattggaaa gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaaaacg
    26401 catatcgtgg aacaactggg tgcagtgcag accccggtaa aaggcgagct gaatgtagct
    26461 atttcctctt ttaattatcc gaaagaagaa tcaccagcat tagaacaaat caagtttgca
    26521 atccaagaag gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacaacgatc
    26581 atcaaattac tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt
    26641 tcgatcaaag aatattcatt gaatgcttta ttaggatcaa tcggctatgt gcctcaggat
    26701 cacttcttgt tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca
    26761 gaagaagaag tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaatta
    26821 ccaaaaggtt atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa
    26881 caaaggattt cgattgctcg agcattgatc aaaaatcctg aattattaat attagatgat
    26941 gcactttctg cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata
    27001 agaaaagaga aaacaactat cattgcagct caccgtttaa gtagcgtgat gcatgcaaaa
    27061 gaaatcattg ttgtcgatca agggaaaatc attgaacgag gaacccatca tgagctgctt
    27121 gcactaggcg gctggtatga acaaatgtgg gagaaacagc aattagaagc gaagatagaa
    27181 ggaggcgagg cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta
    27241 aagaacagaa aaagatcatc aaacgactga tgaagttcgc caaacctttt agaacaacgt
    27301 tcatcgttgc tatcttattt gcgtttgctc tgtctgtgat caatgtccta ttgccaagga
    27361 tcatccaaac ttttatggat gatcatctgg ctaaacagtc ggctacgaca caagttattt
    27421 tgttttttgc tggtatgtac ttatttggag tcctagtaaa aagtatcatt tggtttttcc
    27481 aatggtattt gtattccatg gcttcattga agacctatca gtatattagg gtaaaattat
    27541 ttgaaaagct gcatacacta ggtatgcgtt attttgatca gacaccggca ggatcaaccg
    27601 tttctcgagt gacgaatgac acagaaacgc tatttgaatt ttggtacgtc tttttgatgg
    27661 tattgactgg gatctttgca gtcgtttctt cattcattgc catgtttcaa atcaatggag
    27721 aaattgcctt gtggactttg gtgttcttgc cgattttagg aattgtcatc tggtattacc
    27781 agaaatttag ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaatacaa
    27841 aattgaatga atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt
    27901 tagctaaaga atttgaacaa gtgaacaatg agtatttaga cacgcgctat gcgatgatca
    27961 aaacgaattc gattctgtta agtccgatca tcaattttct atatgcattg gcgattgcgt
    28021 tggcgttgac tttatttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct
    28081 atgcatttac gacttatgtc cagggatttt ttaatccaat gtctcaaatg atggactttt
    28141 tgagcatctt cacagatggg atggttgctg gtagtcgtat cttgaagatt atggatacgg
    28201 aagaatatgc accacaacaa aaccctggag cagatcaaat gattacttca ggtaagattg
    28261 agtttcgcaa tgtaagcttt tcttatgatg gagagcacaa tgtattaaac aatatctctt
    28321 ttgttgctaa tccgggagaa accgttgcat tagtgggaca caccggaagt gggaaaagct
    28381 cgatcatcaa tgtgttgatg cggttttacg aattttatga aggtcagata ttgattgatg
    28441 atcatgacat tcgagaatat ccaatggaag agttacgtaa gaaaatgggg ctggtcttgc
    28501 aagatgcttt cttgttttat ggggatatcg caggaaatat tcggatgatg aatccagcta
    28561 tcacagacga acagatacgc catgcggctg agtttgttca agcaaacaaa ttcatcgatg
    28621 aattaccaaa aggatatcac tcaaaagtga tcgaacatgg tgccagctat tctagtggac
    28681 aacgacagct gatcactttt gcgcgaacaa tcgtgactga tcctaaaatt ctagtattgg
    28741 atgaagcaac agcaaatatc gatactgaaa cagaaggatt gatccaagaa ggattggcga
    28801 aaatgcgaca aggacggaca acgattgcga ttgcccatcg gctgtcaacg attcgagatg
    28861 cagaattgat tttagtttta gacaaaggtc atatagtaga acgcgggaat catgaaacac
    28921 ttctagcgca aaatggtttg tatgcagata tgtacaaact gcaaagtagt ggtatataaa
    28981 agatgttgtg acagtagcat tttgacctaa gcactagagt gaaaataaaa catagcttcc
    29041 catatttttc atctgtgaga gttagtgtcg tagttaccag atttaaaaaa gacgacgtca
    29101 gctagtcaga agtgcctaat agcccctaag aaagcgttga caaaatcgtt tgctttcgat
    29161 aatataaaga aaacgataca acttggatag tgattgcctg atgcaagcta aacctgatga
    29221 gtctcccatg gtttcatgga cggctttttg ggtttttctt tttgttttgg atacagccct
    29281 atatatcatg gaaaggagca aaacatgctg attgagaaag tactgaataa caacgtcgta
    29341 ataacaaggg atgaaagaga tcgggaaatg attgtgatgg gaaaaggatt ggcattcaaa
    29401 aagaaggtgg gtgacccgat tcttgactca tgcatcgaca aaaaatttca gttgacaaat
    29461 gacggacttt cacaacagtt ccaagaattg gcacaagcga ttcctcttga gtacttagaa
    29521 atttcttgtg aaatcattga atatacaaaa gaggctctgc aagtccattt aaatgattct
    29581 gtgtatattt cattgacgga tcacttgtat acagctatcg aacgtcaaaa aatggggatc
    29641 tttgtgccaa atgtattgct gtatgatatc aaacaactgt ttcctaaaga gtatgccatt
    29701 gggaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagacgaa
    29761 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca gcaagacaat
    29821 acctacctgt ttaccacaac aatacaggat attttaaata tcgtccgcta ttactataag
    29881 attacatttg atgaagattc agtccacttt tttcgattcg tcacacacct gcgttttttt
    29941 ctgacacgtg tcctagatgg agcgaaatca gaagatggag aagtttctga ggaattgttc
    30001 caagttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    30061 ttaaccgggg ctttcgacta ccagatgtcc acggatgaaa aaatgtattt aacgatccat
    30121 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaggagga caaattaaca
    30181 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aattaattgg
    30241 agggaataga aatgggaaaa taccaggaat tagcagaaaa catcgtaaag aatatcggag
    30301 gaaaagaaaa tatccgtagc ttgacacact gtgtgacgcg acttcgtttc aaactaaagg
    30361 aggaaaataa agcaaaggat gatgttttga aaaagatgga agggatagtc accatcatga
    30421 aaagcggcgg gcaatatcaa gttgtaatcg ggaatcatgt gccagatgtc tatgcagaag
    30481 tactgacagt agccgggatt tccgatgaag cagaagaaga ggtttcttca agtagtaatc
    30541 tgttcaatcg attaatcgac atattaagtg gctgtttcca gcctttttta ggtgctttgg
    30601 ctgcagcggg aatggtcaaa ggattaaatg ctttgttctt gtttctcaaa ttttatactg
    30661 aaggatcggg tacgtacatc atgctgaatg ggatagggga tgcaattttt tatttcatgc
    30721 cagtgatctt agggtacaca gcagcgaaaa aatttcatct gaatccgatg gtggggctcg
    30781 tgattggtgc agcactttgt tatccgactg tacaaggagc agcactacaa agtggtttcc
    30841 ctgatggcag tcctgcgcct tatagcttgc tgggtcttcc ggcttatgaa acatttttgg
    30901 gtatcccatg ggtgggcagc agttatacta gtagcgtcgt acctgttatc ttcattatcg
    30961 catttgccgg acaagtccaa aaaattgcca agaaatggat tcctgcagtc gtccagacct
    31021 ttttggtttc tttttctgtg ttattaatcg cacttcctgc tggctttttg attatcggtc
    31081 cgattgtcag tatactgaca gatctattaa gcacgggatt ccaagcattg atggactttt
    31141 ctccagctgt gtatggtgca gttctagggt ttctttggca agtactggtg attttcggac
    31201 ttcattggag tattatccct cttgcgatta tgcagctagc gcaaaatggg tggagtcaga
    31261 tgttagtagg cgtttatggc gcgagttttg cacagacagc agtggtattg gcgatgtatt
    31321 tcaagttcaa ggatcaaaaa atgaaggcgc tttgcatgcc agctgtgatt tcaggaatct
    31381 tcggcgtaac agaaccggct atttatggaa tcaccttacc gaagaagaag ccatttattt
    31441 actcgatgat tggtggtgct gtttcaggaa tctatttgat ggcatctgga acaatcagct
    31501 acataatggg cggattaggg atttttgggg tggtaaacta catcacggaa acagacgcca
    31561 gtggtatgtt tcaagcgttt atcggcattg ctattgcttc ggtaatcggg ttttcgctga
    31621 cttttttctt ttggaaggat gaagcaggtg aaacagatgc tatacagaaa aaatcaggta
    31681 aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actaaatgca gcgaaggatg
    31741 cagcttttgc acaaggaaca ctaggtcgag gaatcttaat ctaccctgaa aaaggagaag
    31801 tacgtgcccc ttttgacgga acagttatga ccctgttccc gacgaaacat gcaatcggaa
    31861 tggtttctga aacagggctg gaattgctga ttcatgtcgg attggatact gtgcagttgg
    31921 aagggaaata ttttgaaagc ttagtgcagc aaggagaaag agtgagacaa ggcgatctgt
    31981 tgctacgctt cgacaaaaag aaaatcgaaa aggaaggata cagtttagaa acacctgtga
    32041 tcgtcaccaa ttacacggat tatttggata ttttggaaaa ccaaggggag acggtcgggc
    32101 cagacgatgc acttatcacc gcactgacct gaacggttag gtgaagaatg gactaggctt
    32161 aactattctt tttattccac ttctttattt tcaaaattga aaggctcttt catgatggct
    32221 gagctgatag ggcgtttatc gaatttttga ccgacagaaa ttagatggac taaattctgt
    32281 tgataatttt ctgcaaaaac acaactatat aacacgtcta gaagataaca aattgatgta
    32341 ttgatcgtaa aattaccaat ttttgaatac agtcgctcgc gtgtcgtaag tcgcaaagcg
    32401 gctgtactca ttgcagatag cgttgaatca ccgatactgg tcaaagctaa aattggaaca
    32461 tcattttgtt tcaatatttc tgcggtacga aggatcacat tgttttcacc cgtataagaa
    32521 atcagaatcc cgcacatatc gctcgtacaa ttgtacgctt catagaatga ttcaccaaaa
    32581 gtggtgacaa ttgtgacatc cattttgatg cgcttcattt tcagcatgaa atcttgagaa
    32641 atcaaagtat tcgagttact ggcgaagatc acgatttttt ttgcctggag cagtaaatat
    32701 ttagcttttt gcagttggtc atgatgaatg agagaaagtg tatcatgaat cgtcatctgt
    32761 tctaaagaag ccattttatt tgcgattgtc ataataccgt ctgtggtgga aaaaggaaga
    32821 ttcgcatcta cgtcttgaaa attcccttca agataggctg tttcagctaa aaaagcatta
    32881 cgaaaactga cccagccatc aaaacccatt ttttttgcta cacggatgag tgtagacggg
    32941 tggacataac atcgctcagc tatcgcttgg atggtcattt cgccaagtgc tggagaatcg
    33001 cttaagagaa actggatcac ttcattttct gaaggagaaa aggagatttc tttgattttt
    33061 tcacgtaata acatgtagat accttcttct tattttttta cgtaaattat aacttttttg
    33121 aaaatgtttt gtaaacggat ttatactata taacttaaaa taggcttttt ttgacaagat
    33181 atttacaaaa atccactaga aaacagtaac ttttgtaaat gttactggaa agaaacggat
    33241 ttctattgtt tgttttgttt acaaaaaaga taatgaaagt gtaaaaaagt aaaggagtga
    33301 gaacatggca aaaggaatca aaatcgctac gattggtgga ggcagcagtt acacccctga
    33361 gttgatggaa ggatttatca aaagatatga ggaattaccg atacgagaaa tctggctcgt
    33421 agatgtagaa gaaggaaaag aaaagcttga gatcgttagt cagatgtctc agcgtatgtg
    33481 ggatgcttct ccatatgaag tcaagatcta tgcaactctg gatcgggaag aagcattgaa
    33541 agatgcggat tttgttacga ctcagtttcg tgtcggtcaa ttagatgcac gaatcaaaga
    33601 cgagcggatc cctagttatt atgggatgat tgggcaagaa acaaatggtg ctggcgggat
    33661 attcaaagct tttcgtaccg ttccagtcgt tttggcaatc gttgaggaca tgaagcgttt
    33721 atgtccaaat gcttggctga tcaatttcgc gaacccaagt gggatggtga cagaagcagt
    33781 tatcagatat ggcaaatggg ataaagtgat tggattatgc aatgtgccag tgatggcgat
    33841 gatgacagaa ccagcaatgc ttggcaagaa aaaagaagag ctgatctaca agtttgccgg
    33901 tctaaatcat ttccattggc acaaagtagc agataataaa ggaaatgacc gcacggcaga
    33961 attgattgat cgtttatacg atgaagataa tggattaccg aaaaatatct ttgatatccc
    34021 gtttttcaaa gaacaattaa aacaaatgaa catgatccct tgtggctacc accgctatta
    34081 ctatcgtgaa gaagaaatgc tggaacatgc tttagaagaa tatcaaacga ttggtacaag
    34141 agcgcagcaa gtgaaagaaa cagaagcaga gctatttgaa ttatacaaag atccagaatt
    34201 agaccataaa cctgaacaat tgcaaaaacg aggtggcgct tattattcag acgctgcctg
    34261 tgaaacgatt gcttcaatct atggaaataa agaaacacag atcgttgtat ctacaaagaa
    34321 tgatggagct gttcctgacc tgtcggcaga ttgtgttgta gaagtcactg cctatgttgg
    34381 cgcacaagga gcgagaaatg tagcgtttgg ttctcttcct gctgctgaaa gaggctggtt
    34441 gcaagtaatg aagaatatgg aattgctgac gatcgaagca gctgtgactg gtgattatgg
    34501 gacagcttta caggcgttta cgatcaatcc acttatacga tcaggaaaga cagcaaaacg
    34561 gatcatggat gagttgttta ttgcacacca gcagtattta ccgcaattca aggaaacaat
    34621 cagccgatta aaagaagaag gaatcgaagt tcaagatgaa ctggcaagag aattatcagg
    34681 taatacacat ataatggagg aagtataatg gggttcagaa aagatttttt atggggcggt
    34741 gctacagcag ccaatcaatg tgagggcgct tatgatgaag gtggacgcgg actagcaaac
    34801 gtagatgttg tgccaatcgg aaaagaccgt tttcctgtta tcaccggaaa gaaaaaaatg
    34861 tttgaatttg atgaggatca tttttatcca gcaaaaattg caatcgatat gtatcatcgt
    34921 tataaagaag atattgcttt gtttgcagaa atggggttca agacttatcg aatgtctatc
    34981 gcctggagtc gtatttttcc aatgggggat gagaaagagc cgaatgaagc tggcttgaaa
    35041 ttttatgagg atgtctttaa agaatgccaa aagcacggaa ttgagccact tgtgacgatt
    35101 acgcattttg attgtccaat gcatttgatc aaagaatatg gtgcgtggcg ttccagagaa
    35161 ctcgttggtt tttatgaaaa tctgtgtcga gtcatcttca atcgctataa aggacttgtc
    35221 aaatattggc tgacattcaa tgagatcaat atgatccttc atgcaccatt tatgggcgca
    35281 gggctgtatt ttgaagaagg agaaaatgaa gaacaaatca aatatcaagc ggcacatcat
    35341 gagttggttg caagtgccat cgcaacgaag attgctcatg aagtagatcc ggagaacaaa
    35401 gtaggctgta tgctggcagc aggagcatat tatccataca cttgcaatcc tgaagatgtt
    35461 tgggcttcta gaaaatcaga tcgagaaaat tatttcttca ttgatgtcca gtcacgagga
    35521 gaatatcccg cttatgcatt gaaagaaatg gagcgaaatg gcattcagat cgaaatggaa
    35581 ccaggggatg ccgcattgct gaaagaacat actgttgatt ttatctcttt ttcttattat
    35641 tcttcacgag tttcgactac cgacaacgaa ctcttggaac agactgcagg aaatatcttt
    35701 gcttctgtca aaaatccata tctgcaagca agtgaatggg ggtggcaaat tgatccatta
    35761 ggcttgagaa taacgatgaa tgatatttat gatcgttatc aaaaaccatt atttattgta
    35821 gaaaacggct taggtgcagt cgatacgcca gatgaaaatg gttatgtgga agatgattac
    35881 cgaatcgatt acctggcagc gcatatccag gcgatgaagg atgcagtaga actagacgga
    35941 gtcgatctat taggctatac gacttgggga tgtatcgatt tagtatcagc tggtaccggt
    36001 gagatgaaga aaaggtacgg attcatttat gtcgatcgtg ataaccaagg aaatggcaca
    36061 ttagcccgtt caaagaaaaa atcattcaac tggtataaaa aagtaattga ttcaaatgga
    36121 gaaaatcttt cgaacaattg atcaatgatt tataaaagtc tattgttttt cagcggattt
    36181 ttcgagtata ataaagctat tcgagaaacg caagcagaac aatagacttt ttttcgcatt
    36241 ggctgtaaag tatgcttact tgattaagta aaggagaatc tacatatgta tgatgtaatc
    36301 gttattggcg caggtcctgc tgggatgact gctgctttgt atgcttcacg ttcaaactta
    36361 tccgtattga tgatcgaaca aggtgcccct ggcggccaaa tgaacaatac ggcagaaatc
    36421 gaaaattatc ctggattcga ttcgatcatg ggccccgatc tagcttataa gatgtatgaa
    36481 agtgtctcaa gatttgggac agaaaatgct tatggaatcg ttcaagatat caaggatcac
    36541 ggcagccata aagaagtcat ctgtgaagat aaaagttacg aaggaaaaac agtcattatc
    36601 gcaacaggct gtatccaccg taaattaggc gtacctggcg aggaagaatt tgctggacga
    36661 ggcgtttctt actgtgcagt ctgtgatggt gcgtttttcc gtaacaaacg tctagtagtc
    36721 atcgggggtg gggactcagc agtagaagaa gccatctatt tgacacaatt tgcttctgaa
    36781 gtagtgatcg tccaccgtag agatgaatta cgtgcacaaa agatcatcca agaccgtgca
    36841 tttgcaaatg aaaaaatttc ctttatgtgg gatacagtcg ttgacgaaat cgttggaaac
    36901 gaaatggtag tgacaggtgt gaaagcacgt aatgtcaaaa ccggcaatga atttgattta
    36961 gcagcagacg gagtattcat ctatgttgga ctggatccat taactgcacc ttttagagaa
    37021 gcaggtttga cgaacgaagc tggctggatt ccaacagaca acgaaatgaa aacgaatatg
    37081 ccaggcgttt ttgccatcgg agatgttcgt gaaaaagatt tgcgacaaat cactacagct
    37141 gttggtgacg gcggtattgc tggacaacaa gtgtataaat acttggagtc tttagaagaa
    37201 acaacagcat aagtttaaaa agagtgaaag aacggtttca attgttcttt tactcttttt
    37261 tttcgcgtga aatccactga atccgattta tgtgaatact tgcaatcatg cttgataaaa
    37321 gaatcctttc ttgatttaag cgttttttgg tcctcatact ttcatatcta ataagtgaat
    37381 gctatactta ttaagaacca tatttcagaa agaggtgtta ttttcatgtc ttgggaacaa
    37441 gtgtatgaac aatgggtaca tgaagaaaat ttagaagaaa acttaaaaaa acaattaaat
    37501 gatctgggtg aagatcctga aaaacttgaa gatgcttttt atgcaccgtt ggaattcggt
    37561 actgcaggca tgcggggaat cttaggacct gggatcaatc gaatgaacat ctatacggta
    37621 agacaagcaa cagaaggact agcacgtttc atggatacac aaggtgaaga aacaaaacgc
    37681 cgaggtgtcg ctatcgctta tgactcacgc catcagtcac cagaatttgc aatggaagca
    37741 gccaaaacac tggcacatca caatatcccg tcttatgtat tcgaaagtct gcgtccaaca
    37801 ccagagcttt cttttgcagt tcgtcatttg aagacgtttg cgggaatcat ggtaaccgca
    37861 tctcacaacc cagcggctta caatggatat aaagtctacg gagaagatgg cggacaaatg
    37921 cctcctgcag acgcagatgc attgacgaaa tatgtccgtg aagtcagcaa tccgctaaaa
    37981 gtcgaagtat tatctgacga agaagctaaa catagcgggt tgatcacgat catcggagaa
    38041 gaagtggacg ctgcttatct agaagaaatc aaagcagtga cgattgatcg tgaattggtt
    38101 gaaacaatgg ggaaagattt gaaacttgtg tacacgccac ttcatggaac agggaaaatg
    38161 ttgggcgaac gtgcgttgaa acaagctgga ttcgaacaat tcgtactagt tcccgaacaa
    38221 gctgtggcag atccagattt cactacggtc aaatcgccaa atccagaaga acattcagct
    38281 tttgaatatg ctatacgact tggagaaaaa gagggagctg acttgctgat tgcaacagat
    38341 cctgatgcag accgtttagg cgcagcggtt cgtttgccag atggttctta tgaagtattg
    38401 actgggaacc aaatcggagc attgtttgct caatatattt tggaagcaca tcaatcaaga
    38461 ggcactttac cagaaaacgc agcggtattg aaatcaatcg tttccagcga attgcctaca
    38521 gcaattgcgg aacactacgg tgctactatg ttcaatgtgt tgacagggtt taaattcatt
    38581 gctgaaaaaa tccaacaatt tgaagaagac cattcacata cttttatgtt tggattcgaa
    38641 gaaagttatg gttatttagt taaacctttt gttcgcgata aagacgcgat ccaagcattg
    38701 cttttattag cagaagtagc agcttactat aaaaaacaag gcaaaacgtt gtatgacggc
    38761 ttgcaaacga tttttgaaac ctatggctat ttcgcagaaa aaacgatttc tgtgacaatg
    38821 agcggacaag aaggttctgc gaaaatctct gctttgatgg aaaaattccg taaagaagca
    38881 ccgacagaat ttgccggagc aaaagttatt caaacagaag actttaaaga attgacgaga
    38941 acagaccgtg aaggaaacgt agaaaaacta acaacaccgc catccgacgt attgaagtat
    39001 acattggaag aaaatggctg gatcgctatc cgcccttcag gaactgagcc gaaaatcaag
    39061 ttttatattg gtgtaaaagg aagctctaca gaagacgcag ccgaaaaagt agcctcttat
    39121 gaagcgatta tcaaaaagat gacagaagaa taaaaaactt gagactgatg gaaaaagtgg
    39181 taaactttag aagtcagtct tttttcaagt tggaggtgaa tggattgcca gacacgcaaa
    39241 aatcaaaagt agagaaagtc agtcaattat tcaaaatgtt gagtgacccc actcgtctta
    39301 aaattctgct atatctgaaa gacggggaac aaaatgtgac agcaatcacc caagcagtgg
    39361 aaatggagca gtctgctgta tcgcatcaac tacgtttatt aagagaaaat catgtcgtca
    39421 agtctcatcg cgagggaaaa gcgattttgt atagtcttga tgattctcat gtactggata
    39481 ttttgaacca gacactcaaa catgtggaac atcaataacg ataggtgc
//
LOCUS       DAYYFD010000015        38285 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000015 DAYYFD010000000
VERSION     DAYYFD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.015"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACHA25_000720"
     CDS             complement(451..1761)
                     /locus_tag="ACHA25_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8762047.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACHA25_000721"
     CDS             complement(1773..3191)
                     /locus_tag="ACHA25_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8762048.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACHA25_000722"
     CDS             complement(3431..4999)
                     /locus_tag="ACHA25_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8762049.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACHA25_000723"
     CDS             complement(5003..5413)
                     /locus_tag="ACHA25_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8762050.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACHA25_000724"
     CDS             complement(5410..6333)
                     /locus_tag="ACHA25_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8762051.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACHA25_000725"
     CDS             complement(6330..7208)
                     /locus_tag="ACHA25_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8762052.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACHA25_000726"
     CDS             complement(7195..8532)
                     /locus_tag="ACHA25_000726"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8762053.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACHA25_000727"
     CDS             complement(8535..9638)
                     /locus_tag="ACHA25_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8762054.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACHA25_000728"
     CDS             complement(9712..11220)
                     /locus_tag="ACHA25_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8762055.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACHA25_000729"
     CDS             complement(11186..11878)
                     /locus_tag="ACHA25_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8762056.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACHA25_000730"
     CDS             complement(11900..12874)
                     /locus_tag="ACHA25_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762057.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACHA25_000731"
     CDS             13174..14046
                     /locus_tag="ACHA25_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762058.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="ACHA25_000732"
     CDS             complement(14504..16702)
                     /locus_tag="ACHA25_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762059.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="ACHA25_000733"
     CDS             16934..17926
                     /locus_tag="ACHA25_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8762060.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="ACHA25_000734"
     CDS             17986..19407
                     /locus_tag="ACHA25_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8762061.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="ACHA25_000735"
     CDS             19400..20899
                     /locus_tag="ACHA25_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8762062.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="ACHA25_000736"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="ACHA25_000736"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8762063.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="ACHA25_000737"
     CDS             22491..23174
                     /locus_tag="ACHA25_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8762064.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="ACHA25_000738"
     CDS             23176..24012
                     /locus_tag="ACHA25_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8762065.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="ACHA25_000739"
     CDS             24023..25777
                     /locus_tag="ACHA25_000739"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762066.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="ACHA25_000740"
     CDS             complement(26053..26394)
                     /locus_tag="ACHA25_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8762067.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="ACHA25_000741"
     CDS             complement(26462..28633)
                     /locus_tag="ACHA25_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8762068.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="ACHA25_000742"
     CDS             28844..29701
                     /locus_tag="ACHA25_000742"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8762069.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="ACHA25_000743"
     CDS             complement(29772..30629)
                     /locus_tag="ACHA25_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8762070.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="ACHA25_000744"
     CDS             complement(30614..33316)
                     /locus_tag="ACHA25_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8762071.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="ACHA25_000745"
     CDS             complement(33329..34618)
                     /locus_tag="ACHA25_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8762072.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="ACHA25_000746"
     CDS             34768..35814
                     /locus_tag="ACHA25_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8762073.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="ACHA25_000747"
     CDS             35816..37969
                     /locus_tag="ACHA25_000747"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8762074.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11298 a         7867 c         6809 g        12311 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcttccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcagggc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagcatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taaaccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg actcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga cagagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccatt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 cattt
//
LOCUS       DAYYFD010000016        37387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000016 DAYYFD010000000
VERSION     DAYYFD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.016"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..1009
                     /locus_tag="ACHA25_000748"
     CDS             146..1009
                     /locus_tag="ACHA25_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8762075.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1138..2760)
                     /locus_tag="ACHA25_000749"
     CDS             complement(1138..2760)
                     /locus_tag="ACHA25_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8762076.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3009..3530
                     /locus_tag="ACHA25_000750"
     CDS             3009..3530
                     /locus_tag="ACHA25_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8762077.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3716..5446)
                     /gene="spxB"
                     /locus_tag="ACHA25_000751"
     CDS             complement(3716..5446)
                     /gene="spxB"
                     /locus_tag="ACHA25_000751"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8762078.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5611..6429
                     /locus_tag="ACHA25_000752"
     CDS             5611..6429
                     /locus_tag="ACHA25_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8762079.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6532..6729)
                     /locus_tag="ACHA25_000753"
     CDS             complement(6532..6729)
                     /locus_tag="ACHA25_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762080.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6898..7476
                     /locus_tag="ACHA25_000754"
     CDS             6898..7476
                     /locus_tag="ACHA25_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8762081.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7571..7708
                     /locus_tag="ACHA25_000755"
     CDS             7571..7708
                     /locus_tag="ACHA25_000755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762082.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8521..9024)
                     /locus_tag="ACHA25_000756"
     CDS             complement(8521..9024)
                     /locus_tag="ACHA25_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8762083.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9383..10408
                     /locus_tag="ACHA25_000757"
     CDS             9383..10408
                     /locus_tag="ACHA25_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8762084.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10731..11609
                     /locus_tag="ACHA25_000758"
                     /pseudo
     CDS             10731..11609
                     /locus_tag="ACHA25_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            12085..12201
                     /locus_tag="ACHA25_000759"
     CDS             12085..12201
                     /locus_tag="ACHA25_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8762085.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13129..13467
                     /locus_tag="ACHA25_000760"
     CDS             13129..13467
                     /locus_tag="ACHA25_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762086.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13561..13791
                     /locus_tag="ACHA25_000761"
     CDS             13561..13791
                     /locus_tag="ACHA25_000761"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8762087.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14191..14562
                     /locus_tag="ACHA25_000762"
     CDS             14191..14562
                     /locus_tag="ACHA25_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8762088.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14836..15528)
                     /locus_tag="ACHA25_000763"
     CDS             complement(14836..15528)
                     /locus_tag="ACHA25_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8762089.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15531..16316)
                     /locus_tag="ACHA25_000764"
     CDS             complement(15531..16316)
                     /locus_tag="ACHA25_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8762090.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16553..17953
                     /gene="lacG"
                     /locus_tag="ACHA25_000765"
     CDS             16553..17953
                     /gene="lacG"
                     /locus_tag="ACHA25_000765"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8762091.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17978..18295
                     /locus_tag="ACHA25_000766"
     CDS             17978..18295
                     /locus_tag="ACHA25_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8762092.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18308..19981
                     /locus_tag="ACHA25_000767"
     CDS             18308..19981
                     /locus_tag="ACHA25_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8762093.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19995..21626
                     /locus_tag="ACHA25_000768"
     CDS             19995..21626
                     /locus_tag="ACHA25_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8762094.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22117..23682)
                     /gene="guaA"
                     /locus_tag="ACHA25_000769"
     CDS             complement(22117..23682)
                     /gene="guaA"
                     /locus_tag="ACHA25_000769"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8762095.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23741..24664)
                     /gene="coaA"
                     /locus_tag="ACHA25_000770"
     CDS             complement(23741..24664)
                     /gene="coaA"
                     /locus_tag="ACHA25_000770"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8762096.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24860..25465
                     /locus_tag="ACHA25_000771"
     CDS             24860..25465
                     /locus_tag="ACHA25_000771"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8762097.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25773..26789
                     /locus_tag="ACHA25_000772"
     CDS             25773..26789
                     /locus_tag="ACHA25_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8762098.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26930..28231
                     /locus_tag="ACHA25_000773"
     CDS             26930..28231
                     /locus_tag="ACHA25_000773"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8762099.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28277..28939
                     /gene="deoC"
                     /locus_tag="ACHA25_000774"
     CDS             28277..28939
                     /gene="deoC"
                     /locus_tag="ACHA25_000774"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8762100.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28960..29358
                     /locus_tag="ACHA25_000775"
     CDS             28960..29358
                     /locus_tag="ACHA25_000775"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8762101.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29424..30506
                     /locus_tag="ACHA25_000776"
     CDS             29424..30506
                     /locus_tag="ACHA25_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8762102.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30718..32280
                     /locus_tag="ACHA25_000777"
     CDS             30718..32280
                     /locus_tag="ACHA25_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762103.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32273..33406
                     /locus_tag="ACHA25_000778"
     CDS             32273..33406
                     /locus_tag="ACHA25_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762104.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33406..34362
                     /locus_tag="ACHA25_000779"
     CDS             33406..34362
                     /locus_tag="ACHA25_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762105.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34461..35627
                     /gene="deoB"
                     /locus_tag="ACHA25_000780"
     CDS             34461..35627
                     /gene="deoB"
                     /locus_tag="ACHA25_000780"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8762106.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35651..36469
                     /locus_tag="ACHA25_000781"
     CDS             35651..36469
                     /locus_tag="ACHA25_000781"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8762107.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36486..37193
                     /gene="deoD"
                     /locus_tag="ACHA25_000782"
     CDS             36486..37193
                     /gene="deoD"
                     /locus_tag="ACHA25_000782"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8762108.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11658 a         6530 c         7904 g        11295 t
ORIGIN      
        1 ttatttcttg aagctgacca cttctgtcac aacctcttat tttgattaga acttgcgaaa
       61 ataaaccggt taaagtgtgt ctagcgcaag agatttgcta taatagacga taagggtaaa
      121 atgtaacgag aaggggttga aaaatgtgta tcaaattgta acagattctt gttgtgatct
      181 gccataccat gtattagcaa aagaaaaagt tgatttttta tcaatgaatc tccaattgaa
      241 tgatcaagag ttagtagatg acctcggaaa gacattcaat tatgaccgct acatgaatca
      301 gctgaaaaat ggtgcgatgc ctacgacatc acaaatcaat gttggccgat atttagaatt
      361 tttccgttct tatgcggaaa aacagattcc tgttttatat gtggcttttt ccagtggatt
      421 aagcggctct tacagtagtg cgcttcaagc ggttgaattg ctgaaggaag aatatgaaga
      481 ccctgagatc tatgtttttg atacaaaagc tgcaagtatt ggggaagggc ttttagtatt
      541 ggaagctgta agattgaaaa aagaaggtaa aagcatcgaa gaaaccctag agtggctgga
      601 aagtaattat ttgaaagtcc attcatgggt aacagtagac gatctgaaat atctagaacg
      661 aggcggtagg atttccaaag caagtgctac tcttggcagt ctattgagtg taaagcctat
      721 tattacggtg gataaagaag gaaggctgac gaatgttgac aaagttcgag gaagaaacaa
      781 gtcgatccag acaatcgtca atgagacggt aaaaacaatc gttgcaccac ttgatcaaat
      841 cgtttatgtg gcttacgctg gggatctaga gagcgcggag aaggcaaaac ggcttttgga
      901 agagcagata aagatgaaag atgtgaagct ttatccctta ggaccaacga tcgccagtca
      961 cacaggctat ggctgtatcg cgattttttc gatgggaatc agtcgataaa agcagtaact
     1021 cattctgtaa aaaagcccag aaattgattg taacaaaaac agcgactctt aaaagataga
     1081 tgagcatcct atctcttaga ggtcgctgtt tttttatttt ttaagctaaa taggccatta
     1141 tgcccataac tcgttcaatt gtttttgaat atctgcattt tccaaaaatt catcatatgt
     1201 cgtatcagct cgatcgatca cacctttatc cgaaacggca atgatgcggt ttgccaatgt
     1261 ttggataaat tgatggtcat gtgacgcaaa taagattgag ccagtaaatg ccatcaagcc
     1321 gtcattcaat gctgtgattg attctaagtc taaatggttc gttggatcat ctaatacaag
     1381 tacgttagct ttcgaaagca tcatttttga aagcatgcaa cggactttct ctccaccaga
     1441 taaaacattc actggtttca atacttcttc accagaaaac aacattcttc ctaagaagct
     1501 tcttaagaag gtgttatcat cttcttcttt acttgcatat tgacgcaacc agtctaaaat
     1561 cgttagatca gtatcgaatt ctttcgttgt atcctttggt aaataagaat tacttgttgt
     1621 cacaccccaa cgtactgttc cagtatctgg cgtaatttca cccatgataa ctttgaatag
     1681 cgttgttgtt gttatatcat tttctgcgac gaaagcgacc ttgtcttctt tgctcaaatt
     1741 aaaagatata tcatctaaaa ttttctttcc atcaatcgtt acacttacat tttctacctg
     1801 caataagtcg tttccaatct cacgttcagg tttgaatcca acgaatggat aacgtctaga
     1861 agaaggttgg atatcgtcta aagtaatttt ttcaagcatt tttttacgtg aagttgcttg
     1921 ttttgatttt gatgcattcg cactaaagcg ggcaataaat tcttggagtt ctttgatttg
     1981 ttcttctttt ttggcatttg cgtttgcttg aagtttagcc gctagttgac tagattccaa
     2041 ccaaaaatca taatttccta cataaagttt gattttacca aaatctagat ccgccatatg
     2101 tgtacatact ttgttcaaaa agtgacggtc atgggataca acgatcacag tattttcaaa
     2161 attgatcaaa aattcttcca gccaattgat cgattggata tccagaccgt ttgttggttc
     2221 gtctagcaaa agaacatcag gtttaccaaa tagtgtttgt gctaataaca cttttacctt
     2281 ttgtccacca gttaattcac tcatttgctg atggtgcaat tcttcaggga tgttcagacc
     2341 ttgcagtaaa acagctgctt ctggttcagc ttcccatcca ttaagctcag caaattctcc
     2401 ttctaattca gctgctcgaa taccatcttc gtcagtgaaa tcctctttca tgtaaatcgc
     2461 atctttttct ttcagcactt catacagacg cttgtgtccc atgatgactg tttcaagtac
     2521 agtatagtct tcatagtcgt aatggttctg tttcaatacg gccatccgtt catctggtcc
     2581 catagtaacg gaacctgtgg aaggctgcag atcccctgat aacactttaa ggaacgttga
     2641 tttacctgca ccatttgcac caatcaagcc atagcagttt ccgggggtaa atttaatatt
     2701 cacatcatca aatagtttgc ggtctgaaaa gtgcaaactc acatcattta cagtaatcaa
     2761 tttttttcct cacttttatc attattagtt ttgacaagtc ttatagaatt atatagggga
     2821 aaggtaaaag attcaaggca atcgaacatt ctttgtgttt cacgtgaaac agaatgaaaa
     2881 aaataaaaat aaaagcggag cgaaaatagc tactatagga tttattagtt tgcaacagca
     2941 taatttttga cgttatttat aagagggagt actataaaac tgtaaaagaa atgaaagaag
     3001 gattgaaaat ggatgaaaaa agaaccatcg actggggctc attgattttg ggaattttat
     3061 ttgtacttgt ctcattgtta tcatttcaag accctgtcgg aaatttagtt gcaattgtag
     3121 tagtctttgc gatttttgcc tttattaaag ggatatttga actgtttgta cggaacagat
     3181 taaaagagct aacaggatat aaaggaaaga tgcccttaat aatcgggatc atcgatattc
     3241 tagttggcgt ctttttctta ttcaatattg gtgcaggagt cgcagcattg ccttttgttt
     3301 ttgcagtatg gtttattgct gattcagttt tagcgttatt tactgctgac ttggcaagag
     3361 gagtaagtga aggatattat tggtttacga tcatcgtcaa tattttagga attttattag
     3421 gaattttctt attgtttaat ccaatttctt ctgcgttgac attaagcttt ttagttggat
     3481 tctattttat gttatttggt atcacacata tcgtctatgc atttagataa gtaaaatcaa
     3541 acaaataaaa aagccggctt ccgattacag ttttactgtt gtaattggaa gccggctttt
     3601 ttatcatgga caaatatgca cgaggtgatt tttaggtgtc gagacaaatt tatgcaaaaa
     3661 tgtgagaagc tatatacaca aattttatct taatactcga agcatagcgc agttgtcaca
     3721 acctcttttc atataattca ttcaatactg gcaaatcatg gacttcatat ttttctttga
     3781 atgcttggat ttcttcttca gaatattttc ttggatctaa atgcagatct tctactggca
     3841 atgggcgctt gttattgatc ttcacatcga ttactacagg acgatctgat tttgcagctg
     3901 catcaaaggc aaatcgaagt tgcgagtatt ctgtgactgt aaagctattt gccccgagcg
     3961 cttctccaac tttaccgaaa tctgcatctt ctaaaaatac accaaatttc ttttgttccg
     4021 tatcttcttg ttctgcttcg atgaaaccaa aagaatcatt cgaaaagaca acattaataa
     4081 ttggaagctg atatttgact tgtgtaatga tatcttgcat gaccatagca aacccgccat
     4141 caccacttaa ggtaaatact tgtttttctg ggtagcttag ttgtgcggca attcctcctg
     4201 gcacaccatt acccatagtt gcgaaccatc cagatgtcgt atgtttttgt ttaccgttca
     4261 tattcaataa acgaatagaa tggatagtcg tgttaccgac atctgtgacg aaaatcgcat
     4321 cgtcttcagc aatacgattg atttctttat agacagcctc tggacgaatc ggttcttcct
     4381 ctcgatcttc aaaagatttc aaccaattta cccatttttc tttattttct tgatttgcta
     4441 ggtaccagct atcagcaggg cgagcatccc ctaactcgac aagtctttgc aaagctgtgt
     4501 ttgcatcacc gaggatcgac aaagatgttt catggcgtcg tccaaatttt gacgcatcga
     4561 tatcgatttg gataaattca gcagttggat taaaaaatgc acgtccaaat gggaagtcgc
     4621 taccgacaaa taagatcaga tcggcttctg ccaatgcttc atttccgggt ttagtagcga
     4681 cacgtccagc aaaaccaaga aagttttcat aatcatcagg aataattcct tttgctagaa
     4741 cggaagcgac aacaggaact gaaaaatgtt ctgagaattc tttgatttgc ggaaatccgt
     4801 ttcgcgttcc ttgaccaaca taaagaactg gtttttttgc tttttcaaga taaggcaaag
     4861 cagctagcaa atctttttct tctggtaata tcacacttgt tttgtgagtg tgagcggtgg
     4921 agaaaggttg ttcgtcaatt tcttcaaaac caagatcaac agggatagtc acgatagcca
     4981 cgcctttatg ctcataggcg gctttaatgg cttcatctac tacatgcgga agactttgtg
     5041 gtgtcatgac tgtacgattg tatacgctaa catctgcgaa aataggattt tcatttagtt
     5101 cctggaaaga gttatagttc atggatgtag aagcaacttg tcctaagaac gctactagag
     5161 gcacatggtc catctgtgca tcatacaaac cattgatcaa atgtgtcgca ccaggtcctg
     5221 cagagccgaa gacagcgccg actttccccg ttaattttgc atctgcagcg gctgcgagtg
     5281 cgccgacttc ttcatgacga acttgaatgt agttgatttc ggttttttct tgatatagcg
     5341 catccataat tgaattgaaa gatcctccgg gaatgccgta aatatggtcg ataccccagc
     5401 tttcaagaac ttttaccatt gcgattcctg cattgatttt tgacatggtg tttcactcct
     5461 ttagcaatga tgatacataa attttttgta tctagagtat agaacttttt atcgaaaatc
     5521 ccaactaatt cgtcttttca caaaaatgta gtcatgtttt tgggaatatg ctagagtagt
     5581 acatagatag attcaagaga ggggaatggc atggttgaat ggctattgag acttgaacct
     5641 tggcagcaag cacttgctgg gacagctttt acttatttta tgacagctct tggtgccggg
     5701 ttggtttttt tctttaaaga aatcaaaaaa gaagtgctaa acttgatgtt aggatttgct
     5761 tctggtgtca tgattgctgc aagtttttgg tctttgttag atccggctat tacaaaagca
     5821 gaagaaaatg gagatattgc ttggctagtt gtaagtattg ggtttggttt aggcggtgtg
     5881 tttttatata tggcagataa aacactgccc catatgcatt ttggaccaca acatgaagca
     5941 gaaggattgc ctacccattt aaaacggacg atcttgcttg ttttttctat tacattgcat
     6001 aacattcctg aaggtttagc cgtgggggta gcattcggtg cagcagcaac tgcggataat
     6061 ccgacagccg ctatattagc cgctgtttcg gtggcgctag gtattggtat ccaaaacttc
     6121 ccagaaggag cagctgtatc aatcccgttg cgacaagaag gtctgagtcg taaaaaggcc
     6181 tttgtttacg gacaagcttc tggaatcgtt gaaccaattg ctggagtgat tggagcgcta
     6241 ctagtaacga aagtggagct gctgcttcct tatgcacttg cttttgccgc aggagcaatg
     6301 atttatgtcg tggtggaaga attgatccct gaagcacagc agacattgtc cagcaaacgt
     6361 cattttgcag tttttggcgt catgagtgga tttatcatta tgatgatttt ggatgtggca
     6421 ttaggataaa taaaaaaaat atatatgata tatcaaatag ggccgcgaac ttatatcgcg
     6481 accctatttt tttagtaagt tatatcataa tgaagtgaat ggtatcatac attaataata
     6541 gttttttcta tgtgtatttc ctttgatcca gcgaacaagc cagatgataa gtgcgattgg
     6601 gattagaact ttgaccgcaa accataaaag cccgcttagg acactgacaa aaagactgat
     6661 gacgaagcta gctgcaatta gcactaaaag aaccatgaag atatttaaga acatattttt
     6721 ttgacgcata caaaaccctc ctcttgttca ttctttacta tagaaaaagt ataacaggga
     6781 ttgtttaaaa acacctccaa ctgaagactg atttttactt catctactta aatgatcaaa
     6841 caattgcttt ttctctggtt tatcatctaa aatagagcgg aaaaggagga gaatatcatg
     6901 cctaaaaatc gtcttgaagc atttacagat gctgtaatcg cgattatcat gactatttta
     6961 gttttagaac ttcatgaacc taacggagac acgtgggagg cgctcgctgc attaggttac
     7021 cgatttttta tctatattgt cagttttatc attttagcga tttattggaa caatcatcat
     7081 catttatttc aagttgtaca taagataaat ggacgggtct tatgggcgaa cagtttcttt
     7141 gtttttgcgc tttcgctttt tccgtttgca acttcatgga tcagtgagta catcaatagc
     7201 ctggctccag aaatgactta tggttttgtt accttgaccg ctaatattgc gtattatatc
     7261 ttggcaaaag aacttgtcaa agcaaataaa ggccacgaga aagcattgaa ggcgatgttg
     7321 aataattaca agaaatcgta tctatcgatt ggtttgaatg tgttaggaat tgtcttaggg
     7381 tatttagtag cgccagtagc cgtgctgatc gcaaacatcg tgattctagc tgcttggatc
     7441 atcccaagtc gtaccatcga atcacaatat cggtaaaatg atccgtgttt aaaatttaag
     7501 agaaaaatat agaaaaaata aataaacgat caaatatccg agtgatacta gtattcaaag
     7561 agggatgcag atggatcaaa aaagaattca tgtaggactg attactagtt ttgtcacgat
     7621 gcttatttat ttggatcaag gtcatttgac ttggacagta gttggtgtcg taggattttt
     7681 gacattattc acagcaataa aaaaataaac atctttatgt aatctgatgt tctatctatt
     7741 taagaaaagc atgtttacag cagtatgatt ctgttgtaaa cgtgttttta tttgtggaaa
     7801 atttaatttt tagaaaagtg tatcgaaaaa tagatagtta gtttttccac agtggataac
     7861 ttcgtaagag ataaaaataa aaaattagtc aaagaagggc gtatagattt cttttgatag
     7921 gagatataca acagaaatga ataaatatta acgtgttttc aaacagtata aaacggtgtg
     7981 gtttttggaa taaaaaaata acatatctca ttttacactt ttgttatttt atacttaaaa
     8041 aataacgaca cagaaaatac cgtgtctcag atttctctac tacttctaaa aactactgtt
     8101 tttttccttt gaaatgcctt caaaaagtag gatcaggcag aatatactgg cagaatcaag
     8161 gagaagggag cctaaataag gagaaaaacc atagaaaata taaagtaatt acttataaaa
     8221 caagcttttt atagaaaaat aatatacctg agtagggtat aaattaagac ggaattcctt
     8281 tctagattga gatattgact aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa
     8341 aacccttttt gttaaataag gggaatatag cctgtcagtt tcaaactgca agtcgtcctg
     8401 ctgcaataat atagtagatt agagatgaaa atagattttt ttaaaccaat gactgaataa
     8461 aaagaccgtc atagatacac attggaatgt gtctattacg gtcttttttt acgaacgatt
     8521 ttattgaaga cggacagttc gggtcaaaaa taaaaaagct acgatcagaa ataagaataa
     8581 aatgtatccc aaaataatgg ctagacggtc taacttattt gtcgatagat gtttgtttag
     8641 aagttgatag gcccataaac ctatcagtcc tcctgttgta ttagttatca aatcagtgat
     8701 gtccgctacg ccaataccga ataggaattg aatcaattct gccaagaagc ttacaaggaa
     8761 aataatccct aattttttag aaaaagaaag tctcttgaaa ttgacattca aaagcacccc
     8821 aaaaggaaaa aagacaatca agttataagt ggtctcacgt aataccgtcg tattttcaaa
     8881 agaaaaagta gaaaaaggga cgagattgac attgctgtaa gaataagtga gaaattgtgg
     8941 aagattaaat gatagcttga ataaaatcaa ccataataga aagaataaat aaatccctaa
     9001 taaaaatttt gatacatacc gcatagacat ctcctccatt taaaaagtat accatcgaaa
     9061 accgctgaaa aatcataaaa aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa
     9121 attagaagct tttttttgtt gggaatccgg tttctttaac aaagctaaga tagcgagttg
     9181 cttggaaaag ttgctatctt ttttctgtca aaacgactcg gaatgttcgt tttttgttct
     9241 aatttctcca agttgtttac tttttgatac taagtttatg aaaaatcaag aaatagttgt
     9301 tatactaggt aatgctgaca aacgggattg gcatttcatc ttggttcagc tttttttcca
     9361 attactagga aggagggata gattgttcaa gacttatttt cgtttcaata aaatacaaga
     9421 taatcctttt cgtatcatga atctgatgct acttatcttt attgtattat ggttaggtta
     9481 cataaatcat gattttttga tttcaagttt ttcatcatta gggatcttta cttttttcta
     9541 ttatcaaaac ataccgatga aatatttgct gaaacgtatg tttctgatcg gttgcggact
     9601 gttggttgct ttcgttttgg ggatcttatc gacttatgtt ttatggcttg aaccatttgc
     9661 cgtagctttg gttgcttttt ccagcagatt cgtgttgcga ttattccata tttctaaacc
     9721 aggtggcttg ttttttgcga tgctttctgc catgggaacg agcatgcagc tgccaatcgc
     9781 tcaattaccg atcgtctcac tttatttttt tatgggtgtc gtttttgccc tgattgcggc
     9841 agtgattaca aaattactgg attcacggcc tgagcagaca attgaaaaag caactttaaa
     9901 agaaagattt cacgaagagc ctttagtgat cattgattct gttttctaca gcgctgcctt
     9961 atttctttcc gtttatgtca gtcacggctt gaatctgcat aatccttatt ggttgacctt
    10021 gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg atgaagcatc gacaagtcca
    10081 atatctgatt ggatcgatgg gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt
    10141 tacgcagttg cagaccattt ttctgatcac ttttctatat ggaatcgcac aatttctagt
    10201 tgctagaaat tatgcagttg ctaatatttt tcttaatccg atggcgttga tgctatctac
    10261 acttattcgt ggagcatact tgatttcttt gatcgaatat cgttttttag ggatcgtcat
    10321 aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg gttggtttgc aacattattt
    10381 aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat atgtgccaaa agaggttatg
    10441 acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa agccagattc tcagcattaa
    10501 gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa aattttatat ttattctatc
    10561 aaaaaatttt gtttatgatg gtaactgaat aaactttcct gagatggtca atgctttcct
    10621 tgaaagtctt acagatattc tttcttcata gaaagtttaa gttttatagt tagtgtacaa
    10681 taaaagtatg tttataccat atggtgtccg gataatcagt catttaaaca atgaggttca
    10741 aagatatgaa aaggggaaat ttgatgaaga aaataagcaa tgaaatccaa attctggcag
    10801 aaactttaac tcttcccaaa gtcgttatag agcaaatgat tgtacaagca aaaaacatcc
    10861 ttgatgaggt gtttgagcaa tatagtcatg aactaatgga tcctaataaa gcaaaggcga
    10921 ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt tgcagcatta gctatttatc
    10981 tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc attgaagatt ccaagaacta
    11041 tctatttggc aacaatgaat gcttttacta gatttattca tgaacatttc caagtaacag
    11101 gaagctatcg ctttgaccgt ggtttttgga tttggcatta cactagtggc ttgatctttc
    11161 ggattggtga attggagtac gaaatgacat attttccgaa agggtacacg gatctaagat
    11221 tagaaggaaa gcagtgcctt tctcttcata ttccttccga tgcagattta tctcagaata
    11281 agataagaga tagttacgaa agtgccattc gtttttttga actttatttt cctgattacc
    11341 attaccaagt gatgtacaca gatacttggt tactatcacc caatcttact aaatagttga
    11401 aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg attattatca gttgatgaac
    11461 aggacgatag tggagtccct tggatctttg gacgagtaga tgcccaaata catgattatc
    11521 cagaaagtac gagtttacag cgacaagcaa aagagcaatt gcttgcaggc gagcacattg
    11581 gctctggctt aggtattctt acttcataag tttagtactt cgaaatagtt taaaagagcg
    11641 tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
    11701 agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg
    11761 aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt tagtgttcta
    11821 tcgaacacac agtacgttca ataggtaaac tagctataac aatagatatt aaagttaacg
    11881 gacaagaggg ccttttttag gtagctaaaa cgtaataatg aaaatttatt tccagagata
    11941 ggagtcaatc agctagtctg atcgactgtt ttaacaagtt gactttcatt ttaacttttg
    12001 ataccattta aaacacaaat cgtaattgtt tcgatttgtg ttttttagca tgactacaat
    12061 caaagaagaa agtgggtcgt ccatatggaa aaaacagata tctcaagtgc ataccgccgc
    12121 ttgaaaagcc caaatatcaa aacacgaaaa cgtgcgctga agatcatcaa agatgtgaaa
    12181 cggaatagtg ggaaaaggta agaagaagga aacttaatta aaaaaatagc cgaaaaacgt
    12241 ttttcggcta ttttcaataa tcggtttttg tgtaaggttt ccgaaagaac tgctgttgat
    12301 tttccatttt taacatatga taaattggta gaatctttct tttcaaattg ataccattcc
    12361 ctaattccag ttatatagtt aactacagaa ccacaaagca cgtacgttta taaaagtatt
    12421 atctgtagtt atttttcaat tccctttcaa cctcttattt tgaccagtag tattgatcac
    12481 tttacctttt aacacctttg ttcgtttttg aaacattttt agtcaagaat ggaaacgtaa
    12541 ctacataatc aaaggttttg cgaaatattt catgaaatat ttcatgtaga agcgagcaat
    12601 acggttgcaa ataaccatta tcgtttaata agcagttacg aaagctccca atatctctaa
    12661 cgttcagctc actccatttc ttttcttcaa aaaaatcagt gattcgtatc tctttctatt
    12721 tgtcgaaaaa gttaacttta aaaagtaacg ccgaaaaaat atgcaaaaaa ataaaaaagc
    12781 agttactatt gctagaataa catcaaggaa taataagttt tggttttcag ataatagtcg
    12841 ataagttgtg tattggaatt ttagatacag taataaagta tttttcatga aattttgtga
    12901 gaatcactat tacaagtatc tttaataaaa ttaatggtta aggaggaact taaagtagaa
    12961 tgtaaatgaa tagttgtagt cagtcgattg tgatactaga ctcaaagttg caggaataag
    13021 tttttcaatt tgtgattgga agataaaaaa tgaaaaagaa gggctttttt ttatactttg
    13081 ttgattgtag tgtttatttt ccctctgttt agccaagtaa cattttctat gactcaacca
    13141 gtttcaagtg aggaacatga taaccttttt cgtgctacag aagagtttaa gaaatatcag
    13201 gaaaatattg ttccggagac tccattagct agtgagccaa ttactgatat tgatggaaac
    13261 caaataggat ggtatataca gtacgaagtt atggtggtaa ataatgactc aatagtaaaa
    13321 aatacagtag gcttagagct aacaccgatt gaaaaggtga gttcattgtt aacgtatatt
    13381 tatactgatg aaagtgacga gactgagatc attttaacgg attactcaaa aattgctact
    13441 gataaaaagt atatattgtg gatgtagaca ctagtgaaat actttatgat atagaaattg
    13501 atgaaaattt actagatgta gcacaagaaa tcgatcatca aaagcaggaa gctattcgat
    13561 atgctggtag aaaagggaaa agaaaatatt tattacgtga atgtagcaaa agttagagaa
    13621 gatgaaaatg agtggaaaga attcaagagt cgctactcaa ttaactcgac tccgacattt
    13681 acagtttaca gagaaggatc gatagaaaaa actgtttttt ggactaaaga aagtggaatg
    13741 tctttagctg aagtagaaga gtttttggat tatgttagta tgcaacagta agggagttct
    13801 tattatgaaa aattttgaca catattatgt tattgttttt tcgatatctt tgctgttaat
    13861 attactaact ttaatgcagt ataaagagta tccagagtat tttattttaa cttttgtata
    13921 tttagtaagt agctacacac ttctatataa attcagaagt tcttctagag tttctaaagt
    13981 ttgtttctta attagcttat tgattctata atatttttga tttatatagg aaattaaaaa
    14041 tattatagaa tcaacactca gagaacgggt tggtttttaa tagatttaat aagtactgat
    14101 aatgaaattt ctcctggttg ggaaaacgaa gttggttacc ctgtagaaga attttttcaa
    14161 gattactttg cggagtatgc cgataggata atgagagatt acgtaacaga taaaactcaa
    14221 gaagtcatca atcaatccca gtcattaaat gaattatctg ataaggcgaa agaagaagca
    14281 gttgccatta tttcttcagg gattagtgac tatattgata tgagtggtga aatcaattat
    14341 ggcgataaag acaaaattgt acgcatgata aaagctttta tcaaaacttt tgatgtgaag
    14401 gcagatctta agtttaatga tacttaccta gtaggaaaat taattaatat ccttgggaca
    14461 gagaaagata cggaaacgtt tatttcctct ttgtctatgt tgtttacaga taagcatttt
    14521 tcaactcagc ttggtggaga ggaactagtg gaaatctttt aaccattacg cccagcatta
    14581 ataaaagtat atcaaaaaag tactcctgat gatttttaag attggtttga gaaataatgt
    14641 gaaaacacta tttttaatgt taattataga tagagatgaa cgcgtcatat ggttaaaaga
    14701 atgaaaaagt caaagataca ctgctatatt tcactaagga tatagcagtg tatctttcat
    14761 gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat ttcagttgct tttctttggt
    14821 agttagttga atcgtttaag aattaacgga tgtggcagac aggtgctctg tccaaaaacc
    14881 agtaaaatct ttgatggttc catctttatt tagctgtgcc tgttcaaata ctgttttgtt
    14941 tttaccgaat ggatcaattg caataattgt tccgatcccc gcacgtttgg cagcaagtaa
    15001 tcctgagtaa gcatcttcga taacaagaca atcagccgga gctaagctca gtttgtctgc
    15061 cgctttaaga aaaatatccg gttgcggttt cccagggaaa gagccgtcgt catagacaat
    15121 cttgtcccag tcaaaccagc gggctaaatc gaaaatatca aaataaaaag caacattctc
    15181 tttgacagta gccgtggcaa tcgttaatgg aatctgttgg tcagtcagct ggtcaagagt
    15241 cgcttcaagc ccttttgtca actgaagctc gttttcattt tgcaagcaaa gcatacgata
    15301 atagctttct ttttcttctg acaatacggc gatctcagag gcggtcaaat gctctgaaat
    15361 gaaatgtttc aaaatctccg tattcgtccg accatgaata ttttttaaaa tatcctcgtc
    15421 tgataaacca tcttttgtat gatcatgtat catatgcagc caagcagctt catgcagatg
    15481 agagtccaaa aacattgttc cgttgaaatc aaatataatc cctttcatag ttacgctcct
    15541 atagtggtat tttcggttga atcagcaatt tgcagatcga catattgtgc atagtgctct
    15601 tgaacctccc gcgagatctg gcgatccgta atgagcagat cgaaatcatg caaattataa
    15661 aacacataaa aatcatctcg attgaatttt ttattatcaa cgagtagata tttcttacgg
    15721 gaattattta gagcgatctg ctgtgcttct ccttcttcgg tactgtaagt agagatcgct
    15781 tcgttgtgaa cgccatttga actgataaat gcttttgtaa actttagtct tcttaaattg
    15841 tcgttggtga tcggtccgac aaatgcgccg gtatttttcc ggaaatcacc gccaatcagt
    15901 agaatttctg gtccatctgc ttgagcaaga atatcaaaaa ctggataatt atttgtaatg
    15961 atccgaattt tcttgttcag taaatgcgtc gccaatagtt cgattgttgt acctggtcct
    16021 aagaaaatcg tttctccctc gtgaatcaag gaggctgctg tggcagcaat ttcttttttt
    16081 tcttgaatct gcagagtggc tttttcagtg tgagaaagtt cttgatctaa agtgtaggaa
    16141 agactttgag cccctccatg gacacgcagc agcttgcccg atttttctaa ttcatctaaa
    16201 tctctgcgaa cggtcatatc agaaacatct aattgattga tgatttcatt aacggtaagg
    16261 attccttttt tattgaccat ttctacgata gtaagcaatc tttctttttt caacattgtc
    16321 ttcacctcta atttaaggat aatctatcat ataaacggac aacagtcaat ctaattcgaa
    16381 caaaagtaaa catttagggt ggtttttatg tttgttacaa tagattttat tgctgttttc
    16441 tggtttttat gattatttgt gtttgttttt gttgacaaat gttttggatg cgtttacaat
    16501 gcaatcagtt agaaagagcg aaaactgatt atagatttga ggagagatgt acatgcttaa
    16561 attaccagaa gattttattt ttggcggagc cacagcagcc tatcaggttg aaggagccac
    16621 taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt ttagaggaac aagggcgatt
    16681 cagtcccgat ccggcaagtg atttttatca tcaatatgca aaggacattg aattgtgtga
    16741 acgttttggt gtaaatggtc taagattatc cattgcttgg agccgcattt ttcctgatgg
    16801 agctgggaaa ccaaatccag aaggaatcgc tttttatcat cgtgtgttcg aagaatgtaa
    16861 gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt gataccccta aacgtttgtt
    16921 tgaccatgga gattttttga atcgagaaac aatagaagca tttgtttcct atgccatttt
    16981 ttgttttcat gaattcaaag aagtcaaggt gtggagtacc tttaatgaaa tctatccagt
    17041 tgcaaccaat caatatttgt taggtgtttt cccaccagga atcaaatatg attttacaaa
    17101 gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc cgtgtagtga actatttcaa
    17161 agaaaacgaa cttccgggtg aaattggtgt agttcactca ttagagacga aatatgcggc
    17221 aacagacgcg ccggaagata agcatgctgc ctttttagac gatgcattgt cgatccgttt
    17281 cttattagat gctacctatt taggctatta ttcaactgaa accttgactg ctcttgatga
    17341 gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa gaagatttcg ttgaattgaa
    17401 aaaagcatcc acgcgtaatg actacttagg gatcaatcac taccaatgcc attttgtcaa
    17461 agcttacgat ggagaaaatg caatccatca taacggtacg ggtgaaaaag ggacctctgt
    17521 ttataaagtg aagggaatcg gagaacgtat ttataaagaa ggaattcctc gaactgattg
    17581 ggactggctg atttatccag aaggtctata tgacttgttg ctgcgaatta aaagcgatta
    17641 cccacattac aacaaaatct atatcactga gaacgggatg gggtataaag atcagtttga
    17701 agatggcatt attatggatc agccgagaat cgactatttg agagtctatt tggaatcctt
    17761 aagcaaggcg ataacagccg gggtaaatgt gaaggggtat tttttgtggt cgcttatgga
    17821 tcttttctca tggacaaatg ggtataacaa aaggtatggc ttgttttatg ttgattttga
    17881 aacccagaag cgttacccaa aagaatcagc ttactggtac aagttggtca gtgaaaccaa
    17941 aacgattatt taataagtga taatggagga agaaaagatg aataaagagg aattacaaat
    18001 gcttggtttt gagatcgttg catattcagg cgacgctcgc agtacattat tgaaattatt
    18061 gaaagaagta agacaaggaa actttgaaca tgttgattcg gcgttgaaag aagccgacga
    18121 aaatttaacc cttgcacata attcgcaaac aaaaatttta gcggaagaag catctgggaa
    18181 agagatggag atgggattca tttttatcca tgggcaggat catttaatga ccactttatt
    18241 gctgagagat ctgattcaag actttattgt actgtacaga cagaatcaag gataggagaa
    18301 gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg aaaccatttt ttgaaaaaat
    18361 ctcacgtaat agttatttag gagccgttcg tgacggattt ttaacagcga tgccagctat
    18421 tttattttca agcattttca ttttgattgc tgcggttcct gagatttttg gttaccaatg
    18481 gcctgaatct gtctccacat ggctatggaa agtctataat tattcgatgg gtgttgtagg
    18541 gttactcgtt tcagcaacca ctgctcgctg cttggctgat tcgatgaatc gtcgcttacc
    18601 gaaaaacaaa gtaatcaaca ccacctctgt gatgctggcc tctgtggtaa gtttcctgtt
    18661 gttaagcgtc tctccgattg aaggtggatt ttcaacggaa tatatgggga caaagggact
    18721 actggcttcc tttgtcgctg catttttaac agttaattta tataagtttt gtgtgctgaa
    18781 agatattacc attaaaatgc ctaaagaagt tcctggaacg atttcgcaaa ccttccgaga
    18841 tatttttcct ttctcttttt cagtatttgc ggccgtttta attgatttag gcattcgaca
    18901 aatcttccaa atctcatttg cagaagcaat cattacggtg ctgcagccgc tatttacagc
    18961 agctgacggg tacttaggaa tcgctattat ctggggagcg atggcgctgt tttggtttgt
    19021 cggtgttcat ggaccgtcga tcgtggagcc ggcaattgcg gcaattatct atgccaatgt
    19081 ggaaaccaat ctgcaattgt tcaattccgg tgaacaagct tctaaagtac tgacggtggg
    19141 tttagggaat tttgtaggaa ctatgggagg aactggggca acattggttg ttccttttat
    19201 cttcctactc tttgctaagt ctaagcagtt gaaggcagtt gggaaagctt cctttattcc
    19261 agtaagtttc gctgtcaacg aaccattgct atttgcggca cccatgattt tgaaccctta
    19321 tttttttatt ccatttttgt taacacctgt tgtaaacgtt tgcctgttca agttttttgt
    19381 cgatgtattg ggcatgaaca gctttatgta cgttttgcct tgggcaactc cagcaccgat
    19441 cggtttggtt ctaggaacgg gcatgggtat cttagctttt gttctagcag cggttctaat
    19501 cgtcgttgac ttcctgatct atctaccatt ttgcaaagca tacgatgctg tcttagtaga
    19561 gaaagaagca caaaaagcag aggtgatcgc aaccgaggaa ggtgcttctg ccgatgtacg
    19621 tgaggaagta cctgtggcag cgagtcaagt tatcagcgat gcaaataaag aaatcaatgt
    19681 gttggtactt tgtgctggcg cagggacaag tgggatgtta gctaatgctt taaaaaaagg
    19741 tgcatctgaa tcgaaagcac cgatcagtgc aatggcgggt gcttatggtt ctcattacga
    19801 aatgctgcag gattttgata tggtggtctt agcaccgcaa gtcaattctt actatgatga
    19861 tatcaaagaa gacaccgatc gcttaggaat caagctggtt gcgacaaaag gagcagaata
    19921 tatcggatta acgagagatc ctgaaaaagc tgttcagttt attctgaacc aatttcaata
    19981 aaggaggagc cataatgaaa atgggaattt ggttgcgtaa agctgtttta tttgcagctg
    20041 tgttaggggc tttgggggct gtttcaaaaa ctgttgaagc atctgccagc gaatttattc
    20101 gcggagcaga tatttcgatc ctgtcagata tggaaaaatc tggtgcaaaa tattatgaag
    20161 acgatatttc aaaagatgcc ttagagattt taaaaaataa tggtgtcaac tatgttcgac
    20221 tgcgtttgtg gcaggatcct tataatagta atggagaaag ttatggagca gggacaaatg
    20281 atctggaaac gacgattgac cttgcaaaac gagcaaaaaa tctgggactt aaggtgctgc
    20341 tggattttca ctacagtgat ttttgggttg atcctgggaa acagaatctt cctaaagcat
    20401 ggcaaggtct gacctttgaa gaaatgaata cggcattgta cgactataca aagaacgttc
    20461 tgtcggaaat gaagcagctg gacgtttatc ctgatatggt tcagatcggc aatgaactga
    20521 atagcggaat gctctggcct tatggcaagt cttggggaga aggcggggga gaatttgatc
    20581 gattggctgc ttttttgaag tctggtatcc aagctgttcg agacactcaa cctaaaacaa
    20641 ctccagtaat gctccactta gctgatggtg gcgatacagg agcatttact tggtggtttg
    20701 atgaaatcac gagtcgaggt gtgtcatttg atttaattgg agtgtcgtat tatccgtatt
    20761 ggcacggttc aatgaatgaa cttcaaacca atatgaatgc gatcagccaa cggtatggta
    20821 aagaagtgat cgtagttgaa acagcttatg ggcatacaac agcgaatgcc gatactatgc
    20881 caaatgcttt tggtgaagcc gaagcagctg cggggggata tgaaccaaca ccgacaggac
    20941 aagcagagta tttgctggat cttgccgatc ggattcaggc agtaccgaat aatcgcgggg
    21001 cgggcttctt ctattgggaa cctctttggt acaacggaaa tgtgagttgg gcaacacaag
    21061 caggtatgaa ttatctcagt gttcaatcca ctatgggaaa cgaatgggac aaccaagcga
    21121 tgtttgattt tgctggaaac gccttgccaa gcttgcgtgc gtttaaacaa gcaggggcgc
    21181 aaataaatct agttaaaaat gcaagtttcg aaagcgatgg accaacgacc agtccttcca
    21241 attggcaaac ctggtttcaa caaggaaccg ctaagggtac gatcaaaacg gaagcgggca
    21301 ctttttttgg ggactacaaa ctaacttttt ggcaggatca agcatacgag gcatcagctt
    21361 atcaaaatgt tagtggcata agcaaaggaa cttacacatt gagtgcctat gtaatgtctg
    21421 ggggagagtt tgatactaat cagttatata tcaaaaatta tggtggagcg gaactaaata
    21481 ctgcggttgt taaaacaagt gaatggacaa aaattacaat caaagacatt ccaattacaa
    21541 caggtgtttg tgaaattggg gtttatagca aggcttcagg aaatgcttgg acgaacgttg
    21601 attttgtttc attggtgaaa caatagactg cggatcgttt cgattcaaga tcggaggttc
    21661 ccttcagcca catcgttagc gtagaaggca ggaatcgaac ctgcacctcg cctttgccaa
    21721 aaataaccag aaacgatttc cagtcatttt taccaaaacg agtaaagcac cagacttcat
    21781 ctacactttg tattatgcta aagagagttc aacgatttcg taagaagcaa tgaaaaaatt
    21841 gcaaaaagtt gtgcagataa cataaagttt gtttaagaaa gggtaacctt agaattccgt
    21901 cgtgaatatg atttgagcac ttagatggat agaaaaaagt tctcggaaaa cagttcgttt
    21961 ttcgagaact tctttttatg ataagaatga aacaaattaa cttatattat agtctctttt
    22021 ggtagggaac catcttattt ttaatgaaaa tcaagcattt tagctattga agaatgttga
    22081 tttataggga ttccccctcc atattatgtt ggctctttat tcccactcca ctgttgctgg
    22141 tggtttagaa gtgatgtcat acacgatacg gttgacgtgg gctacttcgt ttacgatacg
    22201 tacagagatt ttttgcaaga cttcccatgg gatacgggca aagtcagcgg tcattccgtc
    22261 gattgaagtt acagcacgga taccgactgt gtagtcgtaa gtacgtccgt cacccataac
    22321 accgacagag cggatccctg gaagaacagt gaaatattgc cagatatcac ggtcaagtcc
    22381 agcagctgcg atttcttcac gcaagatagc atcagattcg cgtacgattt ctagcttgtc
    22441 ttcagtgatt tcgccaagaa cacgaatacc aagaccagga ccagggaatg gctgacgcca
    22501 aacgattgaa tcaggcatgc ctaactgagt ccctaattca cggacttcgt ctttgaataa
    22561 tgtgtttaat ggttcgatca attcaaattg catatcttct ggcagtccac ctacattatg
    22621 gtgagatttg atcgtctgtg ctgtttctgt accagattcg ataacgtcag tatacaacgt
    22681 tccttgtgct aggaaagaga cgccttcttc accagcaagt tttgttgctt cgtcatcaaa
    22741 aacatatacg aattcattcc caatgatttt acgtttttgt tctgggtcag atacaccttt
    22801 caacttgtct aagaagcgat ctcttgcatt gactttgatg atgttcaagc cgaattttcc
    22861 gcctagactt tccattactt gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa
    22921 gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga accccaacga cactagaatc
    22981 aacgccacct gaaagaccaa gcaagacttt tttgtcacca acttgttcac ggattttttc
    23041 tacttgcata tcgataaagt tatccattgt ccagttacct tcacagtgac aaacatcaaa
    23101 ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga cgtacttctg catggaattg
    23161 gataccataa aaattacgtg ttgtgtcttg gatagaagca atcggacaat ctttgcttgt
    23221 tccgactgtt tcaaagcctt caggaacttt agttaccaag tcaccatggc tcatccatac
    23281 tgtttgttgt tttggtgttt ctttaaacaa gacagcttga tcgcttgtta cttctaacaa
    23341 ggctttccca tattcgcggt ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat
    23401 caattgcata ccatagcaaa tccctaatac aggaatacct aaattgaaga tctcaggatc
    23461 gatcccaaat gcattctcat catatacact gtttggtccg ccagaaagaa tgattccctt
    23521 tggattcatc gcttttactt cttctgcagt aatgcggtgg ctcaataatt cggaaaagac
    23581 tccaagatca cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt agtctaatac
    23641 gataatcttt tcaacgttcg tcatatcggc aacgtttgtc aaatttatta cccctatccc
    23701 tttaaatttt atctcaaaat agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa
    23761 tcgaatcgat gatatgattt tcagttttat ggagaattat atcggctctt cctcgtgtag
    23821 gcaagatata ttcctgtaaa tttttcaaat tcacattttt ccatacatct tttgccattt
    23881 taaaggcttc ttcacgatct ccgatagcaa aacgataata ataattctct ggattttgga
    23941 aagccgtatc cagtaacgca ccaaaacgat ccaaatacca tttttcgatc agcacgggat
    24001 cggcatcgac atagacagaa aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag
    24061 gtaattgaag cgtattgatc ccctcaacga tcaagatatc aggctgctgg atcaattcgt
    24121 acttgtctgg aatgatatca tacacatcat gtgagtaaac aggtactttt atctcttcct
    24181 gaccagattt tacacggttc aagaaatcga tcagcatctc catatcataa ctttcaggaa
    24241 atcctttacg gtccatgatt ccacgctctt ttaatatacg atttggataa agaaatccgt
    24301 cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca
    24361 gtctggctgt tgtacttttt ccaacggcta cacttccggc gatcccgata ataaaaggcg
    24421 gtacttctac gtatttatga agaaataaac ctttactcaa gaacaaagat tcaaattctt
    24481 tcatataaag ataaatcaga tgagcaagag gtacatagat atctcgaaca tcctgcatgg
    24541 aaatgcggtc attcaaactt ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc
    24601 cattacgata aaattctcgc cattcttctc ttgccacacg gtagtagttc attggatcat
    24661 tcatattagc ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa
    24721 accccaccac ttaagagaga ttgccgatct ttttgtataa tctttataat agaaagaaac
    24781 ggagtcttat tcttgacttg cgcacggata cataaaagga tagactgttg tcatgaaaga
    24841 aatgagaagg atggaagaca tgacaaatca ttactattct gaacgcccag atacggcgca
    24901 cgattttgaa caatgggctt ttgagttaaa aggcagaacc tttcaatttg taacagattc
    24961 tggtgtattt tcacgtgaaa cagtggatta tggttcacgt gtcctgattg atgctttcga
    25021 ttgggaagag ttacctgatg aaggaaagat cttagatgtg ggatgcgggt atggaccgat
    25081 tgggttagct ttggcatttg cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg
    25141 agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca
    25201 tcaatcgaat atctatgaag cagttcatga agaaacctat gcagctatcg taagcaatcc
    25261 gccgatccgt gctggaaaaa aagttgtaca tgaaattttg acaggcgcat atcctcggtt
    25321 gaagaagggc ggtacactga caattgtgat ccagaagaaa caaggagcgc cgagtgcgca
    25381 aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc acaaaagata aaggatatta
    25441 tatcatcaaa agtgtgaaag aataactagc tgaaaaatag tggagcgacg aaagagtgga
    25501 actgggcgtg catgtacggg tacgtatcag ttcttttttt gttgagaagt ttttaaaaac
    25561 aaagaactat tttttcttca aaaagaaata gtaggaacag agtcgttgtt ttttacgata
    25621 aaataaataa gatacagaac ttgtatagaa ggctgccaag tcggtagata aaggaggttt
    25681 ttatcaaaaa acatactttc cttttttctc tattcttttc ttgggggttt tggtacactg
    25741 atagggaatt tagatagaag ggagccaata ggatgaagct gaccatcaga gatatcgcag
    25801 aaatggcagg cgtatcagta acaactgtct cacagatttt gaataacaaa ggaagcagat
    25861 ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg
    25921 attatttcgc ttcaaatgtg atcaatcgac attcaaagac catcggaatg atcgtccctg
    25981 atgtcactga tttcttcttc tccaaactga tcgaaggtgt agagagctat ttgaatcctt
    26041 tgggctatgt gatcatgtta tgcaattctc gtcatagcca agaaaatgag atcaagtatt
    26101 tacaagagct ttcccaccgc tcagtggacg gaatcttgct ggccacacca aatattttac
    26161 ctgagaaata tgcattagac agcggttttt atcaaaaaat gccaatcatt ttaatcgatc
    26221 gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc
    26281 aagctgtttc gctgtttatt gaaaatggcc atacgaagat cggcatgctg aaggaaacca
    26341 ctggatacta tcaattagaa gaacgtttca acggatatcg tcatgcgctg aaagatcatg
    26401 gcctgccttt taatgggaaa tttgttgaac atggagagtt gactgtccaa ggtggatata
    26461 ctgcatcaaa aaaattactc aaacataaag atatcacggc tattttttgc gggaatgatg
    26521 ccatggcgat cggatgctat caggcgatcg atgaattggg caaaaaaatc ccagaagata
    26581 tctcagtcat aggattcgat ggattaaagc tgtcagaata catgattccg caattgacga
    26641 cagtgcagca gccaagtttt gatattggat tttatgctgc acgtttcctg atcgatacga
    26701 tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta attattcgtg
    26761 agagcgtaaa agtattgaca aagggctaac tcggcggtat attgcttatg taagcattta
    26821 caaaggctag ttagtgcttt ttttattttt agtaaaccag tttactaaag tggtttacct
    26881 ttcctaaaaa taatgtataa taccgaatga agacaaggag gatcaggcaa tgcgaatggt
    26941 ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat
    27001 cgtcacgaac tatacgaatg gaaagattcc agattatcaa gtcagtgcat tattgatggc
    27061 aattttttat caagacatga caaatgaaga aatcacgaac ttaacattgg cgattgctaa
    27121 ttctggtgat gtcatcgatt tgagttctct tgaaggaata aaggtagata agcattcgac
    27181 aggtggcgtt ggtgacacga ccacattgat tttagccccc ctagtagcta gtgtcggagt
    27241 gacagtggct aaaatgtctg gacgcggtct aggttacaca ggtggtacac tagacaagct
    27301 agaagcaatt cccggatttc agatcgagct ttcggatgaa gaatttgtac gcattgttaa
    27361 tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt
    27421 gtatgccttg cgtgacgtca cagcgaccgt tgattctctt ccgctgattg ccagttcgat
    27481 tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga ccactggaga
    27541 cggtgctttc atgaagaaca tcgaagatgc aagacgtctg gcaaagacca tgacgagcat
    27601 tgggaaactg gctaaccgag aaacagtggc ggttatctca gatatgtcag aacctcttgg
    27661 cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc gaaacattac aaggaaatgg
    27721 tccagaagat ctagtagaaa tgtgctacgc cttaggaagc caaatggttg ttcttgctgg
    27781 caaagctaag acaaccgatg aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa
    27841 agcgttagca aaattcaaag aaatgatcca aaatcaaggg ggagacccag cgatcgtgga
    27901 acatccagaa cgaattttga ctgcacgcta cacaatggag cttcctgcta aacaaagcgg
    27961 agttgtgtca aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc taggtgctgg
    28021 gcgtaaaacc aaagaagaag atattgatca cgctgttgga ttgaaactgc ataaaaaaat
    28081 tggtgacact gtgacaaaag gtgagtcact attgacgatc tatagtaacg acgaagaaat
    28141 cacttctgtc attgaattat tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc
    28201 aacgttgatt catgacatca tcacagaata aaggaaaaaa gcggacttta ccgttttaaa
    28261 gaaaagggga aaagaaatgg aactaaaccg aatgatcgac catactattt tgaaagcaga
    28321 cgcatctaaa gaagctgtga tgcaaattat cgaagaagca aaaaaatacc atttttattc
    28381 tgtctgcatc aatccagcat gggttgcgct agcgaaagaa caattacaag gtgagccagt
    28441 agccgtatgt acagtaatcg ggtttcctct aggagcaaac acacccgaga caaaagcatt
    28501 tgaaacaaca gatgccatca ataacggtgc agatgaagtc gatatggtca tcaatatcgg
    28561 tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc
    28621 agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca tcgttattag atagagaaga
    28681 aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca aaacatcaac
    28741 tggtttctca acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg
    28801 accagaaatg ggtgtcaaag cttccggcgg tatccataac gaagaagaag caatggcaat
    28861 gatcgaagct ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg tatctggttc
    28921 aactggtgaa ggctattaat tttttcaaag gagaagctca tggcagaact caaacaagaa
    28981 tggatcaaca cagctattga agcgttagat aaagcctatg ttccttattc ccattttccg
    29041 gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa cattgagaat
    29101 gcatcatttg gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa
    29161 ggagaaaaaa catttacgca tctagtcgta gcggggcaca cccccgatcc tatctctcct
    29221 tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc ggttacgcta
    29281 gtaggagaca atggtgtgac gaaggcaacg accgttcgtg cactgctgcc ttatgcattt
    29341 actgaaaaag atctataact catgttcatt cagtcagctg actgttgaaa aataaaacaa
    29401 ttattttagg aggctttaca gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg
    29461 cactagcagc agggttattt ttaggtgcat gcggcaacaa cggctctact gattcatccg
    29521 gtggcaaaag ctcatcagac acaacaactg cagcattgat cacagacaca ggtggtgtag
    29581 acgaccgttc attcaaccag tcagcttggg aaggactaga aaaatggggg aaagatcaag
    29641 gactttcacg aggaaatgat ggcttccaat atttccaatc ttcaaatgaa tcagattaca
    29701 tcccaaatat tgaccaagca ttgaatgctg gattcaaaac gatttttggt attggttata
    29761 aactaaaacc agcgatccaa gaacaagcaa caaacaatac aggtacgaac tttgttatta
    29821 tcgatgatgt catcgatgga ttagataacg ttgtatctgc aactttcaaa gacaacgaag
    29881 cttcttatct tgcaggtgtt gcagcagctt atacgactga aacaaacgta gtcggcttta
    29941 tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa gccggtgttg
    30001 atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact
    30061 tctctgctcc agataaagga cgttcaattg ctcaaggtat gtatgcacaa aacgcagata
    30121 tcatcttcca tgcttcaggc ggtacaggta acggtgtatt ccaagaagca aaatcattga
    30181 acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag
    30241 gcgaatacac attaaacggc gaaaagaaaa acttcacatt gacatctaca ctgaaagcag
    30301 ttggaacagt agtagaagat ttggcacaaa aatcagcgga cggtaaattc cctggcggcg
    30361 aacacactgt ttatggattg aaagaagacg gtgttggtct aacagaagga caactttctg
    30421 acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag
    30481 ttccagaaac tcctgaagaa aactaaaacc atttctatag ccttgttaga cacatgtttg
    30541 acaaggctat ggtttgatta gaacaagaat tgttcattta tccaagctgc cttgtgacag
    30601 tttggataag ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa
    30661 gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg
    30721 gttcaagaaa atattgtcat agaaatgcag aatattacca aagaatttgg aacattcaag
    30781 gcaaatgaca acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa
    30841 aacggtgctg gcaaatctac attgatgaac gtgctttccg gattattaga gccgacatca
    30901 gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa agccaaccaa
    30961 ttagggattg ggatggtcca tcagcacttt atgcttgttg atgcctttac tgtaacagaa
    31021 aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc ttgaccataa aaaagcacga
    31081 aaagagatcc aaaaagtttc tgaacagtat ggattgtcag tcaacccaga tgcttacgtt
    31141 cgtgatattt cagttggaat ggaacaacgg gtagaaattt taaaaacact ttaccgagga
    31201 gcagatgtac tgatttttga tgagccgaca gctgtattga cccctcaaga aattgatgaa
    31261 ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt cgatcatttt gattacgcat
    31321 aagttagatg aaatcaaagc agtagctgac cgctgtacag ttatccgccg tggaaaagga
    31381 atcggtacag tcaacgttaa agacgtaacc tcacaacaat tagccgatat gatggttgga
    31441 agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct
    31501 attgaaaatc tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta
    31561 gaggttcgtg ctggcgaagt acttggtatc gctggaatcg atggaaacgg gcagtcggag
    31621 ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg
    31681 gaagacatca ctaataaaaa accccgaaaa atcactgaac atggtgtagg acacgtgcca
    31741 gaagaccgtc ataaatacgg gttagtccta gatatgacat tatctgaaaa tatcgcacta
    31801 caaacctatc atcaaaaacc ttacagtaaa aacggcatgc tgaattattc agtaatgaat
    31861 gaacatgcca gagaattgat cgaagaatat gatgttcgaa caacgaatga acttgttccc
    31921 gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga
    31981 gatcctgatc tgttgattgt tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa
    32041 tttattcata aacgtctgat cgaacaaagg gacaaataca aagcagtgtt actgatcagt
    32101 ttcgaattag aagaaatttt aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa
    32161 attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct attgatggcg
    32221 ggctatacct tagaagaagc acgaaaagag ttagaaaagg gggctggcgc acatgaatga
    32281 tcgcagcgaa aaattccgta atttattagt gcctgtactt tcagttgttt taggattgat
    32341 cttaggcgcg atattgatgg ctgcttttgg ttttcagccc atccaaggtt attcttctat
    32401 gctagatgca gcccttggtt cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc
    32461 gttgattttt acagcattag gtttttctgt agctaactca gctgggtttt tcaatattgg
    32521 gctttctgga caagcacttt gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc
    32581 agatctgcca aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc
    32641 gatggcagcg gctgttccag gtttacttcg agcatttttt ggaacaagtg aagtgatcgt
    32701 aacaatcatg atgaactata tcttattata cgtaagcaca tttgctttac atgaagtgat
    32761 gcctgaaagc tatcgctcaa gtttggactc ttccaatatg atcagtcaaa atgcctcact
    32821 gcggttacca tggttaacac aagcttttgg cggctctcgc gtaaactcag gattattttt
    32881 agcaatcatc gcgcttattt tcgtttggct gctgatgaag aaaacgactt taggatttga
    32941 aatccgttct gttggattga atccttttgc ttctgagtat gcaggaatga gtagtaaacg
    33001 tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca gggcttggcg gtgtggttga
    33061 aggattagga acgtatcaaa acttctttgt tcaaacgact tcgctatcta ttggatttga
    33121 cggaatggct gtttccttgc ttggttctgg atcagcaatc ggtattcttt tatcagcgat
    33181 cttgttcagt attttgaaga tcggtggact tggcatgcag acaggagcag gtgttccgtt
    33241 tgagatcgtc aatgtatcga ttgctttgat tatcttcttt gtcgggatca acttcctgat
    33301 tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat
    33361 agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa ctataatgtc gactatcgaa
    33421 ttaattgcct tgatccttac ttctacactg gtttattcaa caccgctaat tctaacgtca
    33481 ctaggcggaa ctttttctga acgaagcggg atcgtaaacg tagggctaga gggaatcatg
    33541 gtcatgggtg cttttagttc catcgttttc aacttgacga tgagtccatc acttggttct
    33601 atgacaccat ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt
    33661 cacgcagtag caacagtcag tttgcgtgct gaccatatta tcagcgggac agtaattaat
    33721 ctgatggcac ctgctttagg tgttttcttg atcaaagcat ggtatggtaa aggtcaaact
    33781 gataacatca cgcagaatct ggggtatttt tctttcccag gattagcaga tattccggtc
    33841 attggaccaa ttttctttaa aaatacattc ttacctgctt attgtgctat tttgattgct
    33901 gtcctagctt gggtgatcat cttcaagacg aaattcggct tgcgcctccg ttcagttggt
    33961 gaaaatcctc aagctgccga cacattaggt atcaatgtat atgctatgcg ttattcaggc
    34021 gtactgattt ctggcttgtt aggcggtatg ggcggtgctg tttttgctca gtcgatttcc
    34081 ggtaactttt cagctggtac gatcgttgga caagggttca tctccatggc tgctatgatc
    34141 tttggtaagt ggaatccttt aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa
    34201 agcttgagta tcattggcgc acaattacca gttatttctt cgattccgcc agtattgctg
    34261 caaattgctc catatttgct gacaatcatt gtgctggttg ttttcttagg gaaagcttct
    34321 ggtccaaaag caaacgggaa gaactatatc aaatcaaaat aatcttttag cgaataaaaa
    34381 gggtgggtta cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc aataaaaata
    34441 aaaagaaaga aggaataatc atgtttaaac gcgtacatct gatcgtcatg gattcagtag
    34501 gaattggaga agcaccagat gccgataagt tcggtgatgt tggttctgat acattaggac
    34561 acattgcgaa agaagcagga ttgaacgttc cacatttaga gcagcttggt ttaggaacga
    34621 tccgtcctct tgaaggtgtg gaatcagtga aagatcatca aggctatgcg acgaaattag
    34681 aagaagtttc tgtagggaaa gatacgatga caggccactg ggaaatcatg ggattgaata
    34741 tcaaaactcc atttcgtgtg tttcctaatg gattccctga tgatctgatc aaacaaatcg
    34801 aagaattttc tggccgtaaa gtcgtatgta acaaacctta tagcggaaca gctgttattg
    34861 acgattacgg tgaacaccaa atgaaaacag gcgatttaat tgtatatact tctgccgatc
    34921 ctgtccttca gatcgcggca catgaagaaa tcattccttt agaagaatta tatcggattt
    34981 gccaattcgt tcgtgatata acaaaagatg atccgtatat gattggccga atcattgcac
    35041 gtccgtacgt gggtgaacca ggagacttca cacgaacaag caatcgccat gattatgcgt
    35101 tgaatccatt cggacacaca gtccttgatt ctttgaaaga agcaggaaag gacgtgattg
    35161 ctgttggaaa gatcaacgat attttcaacg gacaaggaat cactgaatct gtccgcacga
    35221 aaagcaatat ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa
    35281 tcagctttac caatttagta gattttgatg cattatacgg tcatcgtcgg gatgtagtcg
    35341 gctatgcgca tgcgattgaa gaatttgact tacgaattcc agaattgctt gaagcgatgg
    35401 aagaagacga cttgctaatg attacagccg atcacggaaa cgatccgacg tttactggta
    35461 ccgatcatac gcgagaatat gtaccgttat tggtttacag taaaaaaatg aaagaacaag
    35521 gggcattgcc tcaaggatat tattcagata tttcagcaac gattgctgag aactttagtg
    35581 tttcttcgac tgaaaatggc gaaagcttct tgaaattatt aaaataatct ttttaaatag
    35641 gagaaagcaa atgaccaagt taagcgagat gttacaagaa acgacccagt ttatccttga
    35701 taaaggagta aaagaaattg attttggttt gattctaggt tcaggattag gtgaattagc
    35761 agatgaaatc gaagatgctg taatgatttc ttatgatgag atcccgttct ttcctgtttc
    35821 gacagtagtt gggcatgcag gaaagctagt ttatgggtct ctggcaggca agaaagtgtt
    35881 agccatgcaa gggagattcc actactatga aggtcattcc atgcaaacag tgacctatcc
    35941 agtacgtgtg atggccggac taggggccca ttcagtcatt gtgactaatg catgtggtgg
    36001 tgtcaacgaa tcatttgtac caggagattt gatgctgatt accgatcata tcaattttac
    36061 tggtgaaaat cctttgatcg gtccgaatga agatgcaatg ggccctagat ttccagatat
    36121 gagtcaagct tatacgcctg actatcaagc aacagcaaaa gcagcagcag aaaaactagg
    36181 tttggatctt aaagaaggcg tatatatggg ctattctgga ccaacttatg aaacgcctgc
    36241 ggagatccga atgtctcgga caataggagc cgatgcagta gggatgtcga ctgttccaga
    36301 agtcatcgta gcagctcaca gcggattaaa agtgttagga atctcttgta ttaccaactt
    36361 agcagcaggt atgcaagcga atctgagcca tgaagaagtt gtagaaacaa ctcagagagt
    36421 caaacaatct tttaaagcat tggttaaaga aacattgaca ttattgtaat aggaggaaca
    36481 ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat cgccgataag attttacttc
    36541 cgggagatcc tttgcgagct aaatatattg cggagacttt tttggaagat ccagtttgtt
    36601 acaatcaggt acgcggtatg ttaggcttta ccggcaaata taaaggagaa cgtatttctg
    36661 ttcaaggaac aggaatggga atgccatcag ccaccatcta tgcccatgaa ttgatccagt
    36721 cgtatggtgt aaagaaattg attcgagtag gtacatgcgg tgcattgtca aaagacgtcc
    36781 atgttcgtga tttagtacta gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa
    36841 atttccaagc ctttcatttt cctccaatca gtgatttcaa tctattactg aaagcatatg
    36901 agatagctaa agaaaagggc tatacggttc atgtaggaaa tgttctttcg gaagattctt
    36961 tctacaaaga tgatttgaca gagacgttcc aattagctga acttggcgtt ttaggagtgg
    37021 aaatggaagc agcggctctt tattacttag gcgcgaaata ccatgtgcag acattaagtt
    37081 tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat
    37141 ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc gatagcagaa taagaaggaa
    37201 atgaaaaagc tgccctgcat taagtaataa gaacatccaa gcaaaaatag ctcctcatgt
    37261 ttttgacttg gctggactta ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag
    37321 gttgtgaatc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa cagcttttca
    37381 tgtttga
//
LOCUS       DAYYFD010000017        37356 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000017 DAYYFD010000000
VERSION     DAYYFD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.017"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..540
                     /locus_tag="ACHA25_000783"
     CDS             19..540
                     /locus_tag="ACHA25_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8762109.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            637..858
                     /locus_tag="ACHA25_000784"
     CDS             637..858
                     /locus_tag="ACHA25_000784"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8762110.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1015..1179)
                     /locus_tag="ACHA25_000785"
     CDS             complement(1015..1179)
                     /locus_tag="ACHA25_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM8762111.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="ACHA25_000786"
     CDS             1282..1803
                     /locus_tag="ACHA25_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8762112.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2230
                     /locus_tag="ACHA25_000787"
                     /pseudo
     CDS             1790..2230
                     /locus_tag="ACHA25_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2270..2428)
                     /locus_tag="ACHA25_000788"
     CDS             complement(2270..2428)
                     /locus_tag="ACHA25_000788"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM8762113.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="ACHA25_000789"
     CDS             2521..3282
                     /locus_tag="ACHA25_000789"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8762114.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3469..4821
                     /locus_tag="ACHA25_000790"
                     /pseudo
     CDS             3469..4821
                     /locus_tag="ACHA25_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4837..6057
                     /locus_tag="ACHA25_000791"
     CDS             4837..6057
                     /locus_tag="ACHA25_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8762115.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="ACHA25_000792"
     CDS             6092..6769
                     /locus_tag="ACHA25_000792"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8762116.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="ACHA25_000793"
     CDS             6766..7962
                     /locus_tag="ACHA25_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8762117.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="ACHA25_000794"
     CDS             7977..8300
                     /locus_tag="ACHA25_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8762118.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="ACHA25_000795"
     CDS             8304..8639
                     /locus_tag="ACHA25_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8762119.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="ACHA25_000796"
     CDS             8636..9031
                     /locus_tag="ACHA25_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8762120.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="ACHA25_000797"
     CDS             9028..9399
                     /locus_tag="ACHA25_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762121.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="ACHA25_000798"
     CDS             9415..9975
                     /locus_tag="ACHA25_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8762122.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="ACHA25_000799"
     CDS             10102..10413
                     /locus_tag="ACHA25_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762123.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="ACHA25_000800"
     CDS             10437..10628
                     /locus_tag="ACHA25_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762124.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="ACHA25_000801"
     CDS             10628..14473
                     /locus_tag="ACHA25_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8762125.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="ACHA25_000802"
     CDS             14474..15346
                     /locus_tag="ACHA25_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8762126.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="ACHA25_000803"
     CDS             15343..16392
                     /locus_tag="ACHA25_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762127.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="ACHA25_000804"
     CDS             16396..17220
                     /locus_tag="ACHA25_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762128.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..19370
                     /locus_tag="ACHA25_000805"
     CDS             17238..19370
                     /locus_tag="ACHA25_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8762129.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19387..19536
                     /locus_tag="ACHA25_000806"
     CDS             19387..19536
                     /locus_tag="ACHA25_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762130.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19533..19979
                     /locus_tag="ACHA25_000807"
     CDS             19533..19979
                     /locus_tag="ACHA25_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762131.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19981..20118
                     /locus_tag="ACHA25_000808"
     CDS             19981..20118
                     /locus_tag="ACHA25_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8762132.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20155..20448
                     /locus_tag="ACHA25_000809"
     CDS             20155..20448
                     /locus_tag="ACHA25_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762133.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20471..20668
                     /locus_tag="ACHA25_000810"
     CDS             20471..20668
                     /locus_tag="ACHA25_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8762134.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20679..21698
                     /locus_tag="ACHA25_000811"
     CDS             20679..21698
                     /locus_tag="ACHA25_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8762135.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21955..22602
                     /locus_tag="ACHA25_000812"
     CDS             21955..22602
                     /locus_tag="ACHA25_000812"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8762136.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22927..23073)
                     /locus_tag="ACHA25_000813"
                     /pseudo
     CDS             complement(22927..23073)
                     /locus_tag="ACHA25_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            23312..24004
                     /locus_tag="ACHA25_000814"
     CDS             23312..24004
                     /locus_tag="ACHA25_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8762137.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(24286..24358)
                     /locus_tag="ACHA25_000815"
     tRNA            complement(24286..24358)
                     /locus_tag="ACHA25_000815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24323..24325),aa:Lys,seq:ctt)
     gene            24739..25308
                     /locus_tag="ACHA25_000816"
     CDS             24739..25308
                     /locus_tag="ACHA25_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8762138.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            25295..26155
                     /locus_tag="ACHA25_000817"
     CDS             25295..26155
                     /locus_tag="ACHA25_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8762139.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            26345..27049
                     /gene="yycF"
                     /locus_tag="ACHA25_000818"
     CDS             26345..27049
                     /gene="yycF"
                     /locus_tag="ACHA25_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8762140.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            27054..28889
                     /gene="walK"
                     /locus_tag="ACHA25_000819"
     CDS             27054..28889
                     /gene="walK"
                     /locus_tag="ACHA25_000819"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8762141.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            28886..30202
                     /gene="yycH"
                     /locus_tag="ACHA25_000820"
     CDS             28886..30202
                     /gene="yycH"
                     /locus_tag="ACHA25_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8762142.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            30203..31072
                     /locus_tag="ACHA25_000821"
     CDS             30203..31072
                     /locus_tag="ACHA25_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8762143.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            31127..31936
                     /locus_tag="ACHA25_000822"
     CDS             31127..31936
                     /locus_tag="ACHA25_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8762144.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(32039..32668)
                     /locus_tag="ACHA25_000823"
     CDS             complement(32039..32668)
                     /locus_tag="ACHA25_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8762145.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            32838..34130
                     /locus_tag="ACHA25_000824"
     CDS             32838..34130
                     /locus_tag="ACHA25_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8762146.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(34206..34868)
                     /locus_tag="ACHA25_000825"
     CDS             complement(34206..34868)
                     /locus_tag="ACHA25_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8762147.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            35093..35377
                     /gene="groES"
                     /locus_tag="ACHA25_000826"
     CDS             35093..35377
                     /gene="groES"
                     /locus_tag="ACHA25_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8762148.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            35428..37053
                     /gene="groL"
                     /locus_tag="ACHA25_000827"
     CDS             35428..37053
                     /gene="groL"
                     /locus_tag="ACHA25_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8762149.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT        12894 a         6308 c         8098 g        10056 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtcaaa
    18721 ttgactggct caagatcgaa aaaggcaata ctcgaacccc gaatattagt gagtataaat
    18781 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    18841 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    18901 caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta
    18961 atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta
    19021 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accacattaa
    19081 cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta
    19141 tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa
    19201 ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt
    19261 ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa
    19321 actcaagtta tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga
    19381 atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg
    19441 aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat tctaaattgg
    19501 aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta
    19561 ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag
    19621 atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa
    19681 tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt
    19741 taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga
    19801 tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag
    19861 aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata
    19921 gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt
    19981 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
    20041 gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat cattagcaaa
    20101 gaagaaagcg catcttaagt gatgcgtttt tatttaaggt aaaggagttg tcacatgatc
    20161 aatttagggg aatggggaac aatcgcagga tcaattactg cgattgtttc tttgatttta
    20221 ttagtaataa gaccagtcac tgcatctttc tcgaagatta ctgaaactct ttcaaaagta
    20281 agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga tcgattgatg
    20341 attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta
    20401 gaacacacac aacaaattaa aactttattt agagaaaggt ctcggtaaaa aagaaaggag
    20461 ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga cagttttaac
    20521 agtattgcca gctttatctg tattagtagc cacattaggc aaagcatatg gatggaatgg
    20581 aacagatatg gcagttctga ctatcaatgc agcagcaaca tttttaggtg ttatcactgg
    20641 tgtgtcggct tataatttga aaaaatagga ggaaacaaat gaaaaagaaa attactatta
    20701 ctgcgatgag cctattaacg gctctttttt tattgccgat taatgggttt gcctatacga
    20761 ttaacaatga atttaattta ggtgtaaacg aaggtagttc tcaagtagca aataatcagt
    20821 acattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
    20881 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
    20941 atcaagtcgg tcaacctggt tatgtacagt acggtgctgg ttcgtatgct aatgcaaata
    21001 gtcctgtgca gattgagtta caacacacac atgataaagc aacgtttgag aaaaactaca
    21061 aggcatacgt tgaattagct agagattcag caatgaaata tggtattcca cttacactag
    21121 acacaccgta taatcaacca gggatcaaat cgcatttatg ggtaacacaa aatatttggg
    21181 gcgatcatac agatccttac ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag
    21241 cctatgattt ggctcatgga tttaccgatg aaaatccgac aacttcagat gataaaccag
    21301 tcattgatcc aactcgagca ggtgctgcaa atcctaccct gacagatgga acaaattacg
    21361 cccacattga tcagttcgga gaaatcgaaa acgcaaactt acatgtggct ggttggcaca
    21421 tcgctaacta taaatacgag tatatcttca ttatggatta caatactggg aaagaattag
    21481 ctcgagtaaa agctgatgga atatatagac cagatgtaaa tcaagcttat aatactttag
    21541 gaaatgttgg ctatcatgta tcttttaaca tgcgcaactt ccctaataag aaagtctatg
    21601 taatgatgcg ggcaacgaat gatccagaag ggaacactaa aggcggagca caagattttc
    21661 atgataaacg ctggtattta aatattccgc aacgataaaa atagcccctc gtttttgagg
    21721 ggaagtacat aatgcaaatt tgtccataac ttattgatta tcttgtaaaa atgcggtaat
    21781 ttaatagtta gtgagaagag gtgcttaatt atagttaaaa aagaggaaat agaagcagca
    21841 tgtaaccaag caaatgttag gtttattgga gaagctttat ctggatatcc taagtataaa
    21901 tacttacaca actggttaca aagtaaaagt ttttatctta ataaagaaag atgcatgcct
    21961 aataaaaatt atagggttta taaaagagga actattgtat atgttgattt tggggtgaac
    22021 gtgggttatg agttatctgg taatcatttt gctattgttt taaataataa agataattcg
    22081 aaaaatggtg ttgtatgtgt agtaccaatc tcttcaaaaa agaaaagtaa ttatgtatct
    22141 gtaggaaaaa tattagaagt tgctagtatt aaacaattta taagccaagc tgataaatta
    22201 aaagagaaac ttaggataat tattttactt tcattaaata gaggcatcat tgatgaaagc
    22261 gatttgaata gttttattaa agatattgaa gctaaaggtg aaaagctttc agtggaagaa
    22321 gtagaaaaac gagctgtaaa acataatttg gtttgcgaga caagggaaca agtaattaaa
    22381 gaaataaaat cattatctga agatatggta aaatatcaga aggtgtttaa ttgctacaag
    22441 aaatatacac aagaatccta tgtaatgcca cttaatatcc aaacgattag taaaaataga
    22501 attagaagaa ttaacaagtt tgatccttct ggaaatatga aggcacctgg aaatgtcctc
    22561 aatgaagtag atgaaagttt aaaaaaatat tttacaaagt aaacgatatt tttgttgaca
    22621 tttttctgtt cgttagttac catgtagtta tagaaggacg taagcgccta tttttaatgt
    22681 acacatcggg acgtatgcgc ccacatagaa acatagttga gagacatcta tatggtgtct
    22741 ctttttttat taatatgcaa aaaagtctag ttttttcaat aatatttatg taataataaa
    22801 ttagccacca caacaaagaa tgaaatccat tattatctag tctagtccat tctttttgtt
    22861 tgcagtattt gtgatggctt tccgtaccct tagctcagtt ggtcagagca gacggctcat
    22921 aaccgtccgg tcgtacttct cgtaatccag gaaggccgtc agctccgtcc gcagtagcgt
    22981 gtttacggcc agctccagat gggagcggaa taactcgttc aaatcacctt tggtggctag
    23041 agtctgcagg agttctgtaa taaaatcgtt catagggaag tcctcttttc tgtgaatggg
    23101 tgtggtaact ttattctaca gaagggactt ccttttttct atttacacaa aatattttat
    23161 actctcaaat gtttgtatgc agttacatct gactttattc gaattaagga aaagatttat
    23221 aaaaataaaa taaaaaaata tatatttaaa ttgacttatt gtgaaatcat taactataat
    23281 tagttcagtt tatgttaagg agaatgatga tatgaataag aaaattgtag taagtggatt
    23341 aattataggt ggattagtgt tagcagggat cgcagggaaa aactttgccg atgacatttt
    23401 gttatggggt ggagaagata atattgaaca aatcaatcat aatttggagt cattagataa
    23461 agcactagaa aataaagagc aaaatattag tagtttaaat tcacgattat cttcaaataa
    23521 tcaagaactt gagcaagcaa aggctaacgt tgaagaatat aaacaaaaag tgttgacttt
    23581 ggaaaatgat aaaaatcaat taactataga gaaaacaaat ttagagaatc aattatcagg
    23641 aaaaaatgct gaattgcaag gtaaacaaaa tgaaatcaat gctaaaattg atgaaataaa
    23701 tcaaaaaata gctgaaataa atcaattaaa cactgattgg aataatcgtt tacaaacaga
    23761 acaaaataaa tttaatgatg ctcaaaatac tgttaatagt ttaaatcaac aaattaatca
    23821 attgtcaaat gaaaaaaata ctattaaaaa ccaattgcaa agtactgaac aaaaactaga
    23881 aaaagcgcaa tcagaaaaca gtaatttgaa taattatatt gaaaaactgg aaaaagccaa
    23941 aagcgatgta gaagacacag caaacagatc acaagaaatc gtgactgaac atacaggaaa
    24001 atagatgcat tttttagttt aaatttcttt aacaaaatag tattttgttg ctaaataatt
    24061 tttatttgaa tattctacaa tgatatctcc cctcaaaatg gaggggaagt acatactgtg
    24121 ataatctaca ggtttattga ctgattagga tgttattaaa aaataaaata ctaaaatgca
    24181 tacccgaatt tcatacccga aaaataaaaa atgccaccac gtacactctt ttcgaaacta
    24241 cctaagtagc caagaatgta ctggtgacgt gggtttggca cccaatgggc cgtgaggggc
    24301 tcgaacccgc gacccgctga ttaagagtca gctgctctac caactgagct aacggcccgt
    24361 acaaaaagta cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag
    24421 aaaatcagaa aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct
    24481 cgctattata taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt
    24541 ttttcatttt ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa
    24601 aagacaagaa ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca
    24661 cctagttaaa gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg
    24721 aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa
    24781 ctagaaacat tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac
    24841 caagtcagaa cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca
    24901 ttgcttacct cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt
    24961 aaaaaaagat ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa
    25021 gtattgacta ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt
    25081 cgtgaagcct ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa
    25141 ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt
    25201 gctgtaagag cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt
    25261 ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt
    25321 agtagattca ggtacggatg tgccagaaga attgataaaa aaatacgaga tgtatatgat
    25381 cccattgaaa attatttata aagatcaggt atatacagat aaaattgaca ttacctctga
    25441 agagatctat caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc
    25501 gatcacagaa atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac
    25561 aatttccagt ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga
    25621 agaattggaa gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc
    25681 gatccaagca gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct
    25741 gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca
    25801 aaaaggtgga cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc
    25861 aatcatctca tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa
    25921 aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt
    25981 caacttagct gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact
    26041 taaaagagag ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt
    26101 agtccacact ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa
    26161 aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa
    26221 aataataaag tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg
    26281 aaacaaaagc agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct
    26341 ctttatgaaa aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt
    26401 caatttagcc aaagaaggct atgatgttta tactgcatat gatggggaag aagccttgga
    26461 aaaagttgct gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga
    26521 cggcttagaa gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac
    26581 agccaaagat tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt
    26641 gaccaagcca ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg
    26701 tgccacggct gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt
    26761 gacgatccat ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca
    26821 tcgtgaattc gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga
    26881 acatttatta caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt
    26941 aactgtacgt cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt
    27001 gacacgtcgt ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa
    27061 gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt
    27121 tattgatctc gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga
    27181 tcaatacatt tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc
    27241 cggaattgaa tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat
    27301 ttattgaaaa cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga
    27361 tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg
    27421 ttgatatcaa tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg
    27481 tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt
    27541 acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct
    27601 ttactgcctc actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt
    27661 cgattacaca gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact
    27721 acagtcggaa agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca
    27781 atcagttagc tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt
    27841 tggatagcgt gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag
    27901 tcattacgat caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg
    27961 gacaatcgat tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag
    28021 aagatccaaa cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga
    28081 tcttgcgagt agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag
    28141 ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct
    28201 ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat
    28261 tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag
    28321 atgaaacaga ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata
    28381 caggtaatac ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc
    28441 ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat
    28501 ttacgcaaag agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata
    28561 atatcatgaa taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat
    28621 tagaaacgca taataacgtg gttttaagta tcaccgatca aggattgggg attcctaaaa
    28681 aagatctgaa ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac
    28741 aaggtgggac aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg
    28801 ctatttgggc agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg
    28861 aaccttatga ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact
    28921 cattttgatg gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg
    28981 aaaaagttca atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg
    29041 aaaaacttcc gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca
    29101 agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt
    29161 tggaaagctt tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct
    29221 gaactctggt tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc
    29281 atttgattta gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac
    29341 tagaatacaa ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct
    29401 cgtatatgaa gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa
    29461 agacacaaaa tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac
    29521 gaaacattcg atcaaactga aaaagtatag ttatatcctg tcacagcaac cctatacact
    29581 ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga
    29641 attgattttt tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga
    29701 ttttcgtgga gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc
    29761 ctatattagc aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa
    29821 tacgatcgat tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg
    29881 acagattggt gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac
    29941 cagtttgaat acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac
    30001 gaatgaaatc atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt
    30061 gatcgttggc tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc
    30121 tgaatggtat gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt
    30181 acctggattg gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc
    30241 ttttttaggg ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa
    30301 caatgtcatt cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat
    30361 cacttacacg gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca
    30421 gaccaatctt agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc
    30481 aagtggtgtg tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga
    30541 aaagaacgtg atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa
    30601 agacaatatt ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga
    30661 agaaccacta gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac
    30721 agcaaaaatc gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat
    30781 gcagacgcat ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga
    30841 ggaagcaatc aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg
    30901 gcgtcagcta gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt
    30961 ttggcatgta gcgatcgaaa caagcgataa agtggtccaa attgagagtg tcaacgcttt
    31021 cagcaatacg atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga
    31081 gtataatgaa aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa
    31141 accagctttt aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga
    31201 aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt
    31261 gatggctcaa attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca
    31321 tcgagatcat atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc
    31381 caatcagcag acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa
    31441 acatctattt gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg
    31501 cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt
    31561 cgtcatgttg acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc
    31621 cgatggttat ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc
    31681 gtggaattta aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc
    31741 acttgtcatg actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag
    31801 caaggaaaac aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa
    31861 agattttggc gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga
    31921 gctattttcg atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa
    31981 tcagggttgg ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt
    32041 atcgactgac tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta
    32101 atgcaatcgc gtaatctgcc atgctgatcg tactgtcgcc agcagcgttt ctttgtaaat
    32161 aatcatcact taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg
    32221 gactgatata agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca
    32281 tattgaaagc tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg
    32341 tcttttctgt atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg
    32401 ttccttcaag catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt
    32461 tgggagcgtt gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact
    32521 caaataaatc cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga
    32581 ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc
    32641 cagtagctcc aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt
    32701 aagcagttgc tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct
    32761 ttcatggttc gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa
    32821 gttgaaggag ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat
    32881 ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa
    32941 taattattgt tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc
    33001 agtggataga caaaacagga ttttctatgt attttacaca agtataccag gctacatttg
    33061 atgtgatagc cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca
    33121 tcgatcgctt accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac
    33181 taaatactca gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa
    33241 ctgggctcat catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc
    33301 aaagagagaa gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg
    33361 ttatagtcac ttttacgctt atcggttcag cggtttttca acagttgttc agaatgactg
    33421 ttgttcaagg agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt
    33481 ttggtgtgct tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat
    33541 ctgcgatggt caatgggttg gttagtcccg tcttgcaagc gaattcatta gcgaatgcag
    33601 aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc
    33661 aatttctcga tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt
    33721 ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac
    33781 cagccttttt caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga
    33841 tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt
    33901 tatattttgg attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga
    33961 tcctctcggg tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt
    34021 caattagttt tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa
    34081 aggaaagaca gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa
    34141 tactcctgtc gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt
    34201 catgtttata ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt
    34261 cctacgtgtg tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag
    34321 aagaaaccta ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc
    34381 aaagagctga tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata
    34441 ctgcggcgga atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa
    34501 gcgggtgctt ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg
    34561 ccaaacaata cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt
    34621 ccaaccaatc cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc
    34681 gctaagggtt cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac
    34741 atgactgcag ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg
    34801 atcaagggtg agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt
    34861 aaagacatgt aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat
    34921 tgatttcaat tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg
    34981 atttaaaagc ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc
    35041 gaaggttaag agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa
    35101 accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg
    35161 aatcgtttta gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg
    35221 tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca
    35281 agttatgttc gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat
    35341 cgtcgcagga aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc
    35401 aaaaataatc aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca
    35461 cgcgcagcta tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga
    35521 cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat
    35581 ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa
    35641 cttgtatcag aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca
    35701 actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat
    35761 cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat
    35821 aatatctcaa ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg
    35881 ggttctgata aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt
    35941 gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg
    36001 cagtttgatc gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct
    36061 gttctagaaa acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt
    36121 ctgccattgt tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat
    36181 gtagatggtg aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta
    36241 gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct
    36301 attttgacag gtggtacagt aattactgac gatctaggtt tagaattgaa agatgtaaca
    36361 atcgaaaact taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta
    36421 gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt
    36481 gctgaaacaa cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca
    36541 ggtggtgtag ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa
    36601 ttacgcattg aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca
    36661 ggtggtggta cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga
    36721 gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc
    36781 gctgaaaacg ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta
    36841 ggcactggtt tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta
    36901 gacccaacta aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta
    36961 ttaacgacag aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca
    37021 atggatccat caatgatggg cggtatgatg taagaaaaag caacttgctt atctacaact
    37081 gatcattagt gaaagacaga taaaaagagg ctggaacaga agcgtttaac tccaagaaat
    37141 aagaagaaat tcacggaaat tgcttttcaa atttttgtga atttcagctt atttccgaag
    37201 gagttgcttc tgcttccgct gtttatacgt gtttagagcg tgagacaaaa gtgttcttta
    37261 cttttgtccc acgctctttt ttgtgtgtaa ttataatgaa aatagttatt tttaatagtt
    37321 ataatatttt atctattaaa agtaaatatc cattca
//
LOCUS       DAYYFD010000018        36519 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000018 DAYYFD010000000
VERSION     DAYYFD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.018"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1102..1416
                     /locus_tag="ACHA25_000828"
     CDS             1102..1416
                     /locus_tag="ACHA25_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762150.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(1469..2242)
                     /locus_tag="ACHA25_000829"
     CDS             complement(1469..2242)
                     /locus_tag="ACHA25_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762151.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            2347..2592
                     /locus_tag="ACHA25_000830"
     CDS             2347..2592
                     /locus_tag="ACHA25_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8762152.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2748..3599
                     /locus_tag="ACHA25_000831"
     CDS             2748..3599
                     /locus_tag="ACHA25_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8762153.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            3632..4309
                     /locus_tag="ACHA25_000832"
     CDS             3632..4309
                     /locus_tag="ACHA25_000832"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8762154.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            4797..5762
                     /locus_tag="ACHA25_000833"
     CDS             4797..5762
                     /locus_tag="ACHA25_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8762155.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            5784..5996
                     /locus_tag="ACHA25_000834"
     CDS             5784..5996
                     /locus_tag="ACHA25_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8762156.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            6071..6547
                     /locus_tag="ACHA25_000835"
     CDS             6071..6547
                     /locus_tag="ACHA25_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8762157.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            6544..8352
                     /locus_tag="ACHA25_000836"
     CDS             6544..8352
                     /locus_tag="ACHA25_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8762158.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8429..8644
                     /locus_tag="ACHA25_000837"
     CDS             8429..8644
                     /locus_tag="ACHA25_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8762159.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            8736..9599
                     /locus_tag="ACHA25_000838"
     CDS             8736..9599
                     /locus_tag="ACHA25_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8762160.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            9636..10172
                     /locus_tag="ACHA25_000839"
     CDS             9636..10172
                     /locus_tag="ACHA25_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8762161.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            10186..10608
                     /locus_tag="ACHA25_000840"
     CDS             10186..10608
                     /locus_tag="ACHA25_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8762162.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            10538..12937
                     /locus_tag="ACHA25_000841"
     CDS             10538..12937
                     /locus_tag="ACHA25_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8762163.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            12969..14960
                     /locus_tag="ACHA25_000842"
     CDS             12969..14960
                     /locus_tag="ACHA25_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8762164.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            14983..15234
                     /locus_tag="ACHA25_000843"
     CDS             14983..15234
                     /locus_tag="ACHA25_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8762165.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            15224..16453
                     /locus_tag="ACHA25_000844"
     CDS             15224..16453
                     /locus_tag="ACHA25_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8762166.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            16450..18531
                     /locus_tag="ACHA25_000845"
     CDS             16450..18531
                     /locus_tag="ACHA25_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8762167.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            18680..22600
                     /locus_tag="ACHA25_000846"
     CDS             18680..22600
                     /locus_tag="ACHA25_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8762168.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            22601..23545
                     /locus_tag="ACHA25_000847"
     CDS             22601..23545
                     /locus_tag="ACHA25_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762169.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            23549..23752
                     /locus_tag="ACHA25_000848"
     CDS             23549..23752
                     /locus_tag="ACHA25_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8762170.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(24052..24288)
                     /locus_tag="ACHA25_000849"
     CDS             complement(24052..24288)
                     /locus_tag="ACHA25_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762171.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(24471..24842)
                     /locus_tag="ACHA25_000850"
     CDS             complement(24471..24842)
                     /locus_tag="ACHA25_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8762172.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(24839..26167)
                     /locus_tag="ACHA25_000851"
     CDS             complement(24839..26167)
                     /locus_tag="ACHA25_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8762173.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(26128..26457)
                     /gene="mobC"
                     /locus_tag="ACHA25_000852"
     CDS             complement(26128..26457)
                     /gene="mobC"
                     /locus_tag="ACHA25_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8762174.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(26715..27085)
                     /locus_tag="ACHA25_000853"
                     /pseudo
     CDS             complement(26715..27085)
                     /locus_tag="ACHA25_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            27230..27415
                     /locus_tag="ACHA25_000854"
     CDS             27230..27415
                     /locus_tag="ACHA25_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8762175.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            27573..27740
                     /locus_tag="ACHA25_000855"
     CDS             27573..27740
                     /locus_tag="ACHA25_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762176.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            28358..29017
                     /gene="vanR-B"
                     /locus_tag="ACHA25_000856"
     CDS             28358..29017
                     /gene="vanR-B"
                     /locus_tag="ACHA25_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8762177.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            29020..30381
                     /gene="vanS-B"
                     /locus_tag="ACHA25_000857"
     CDS             29020..30381
                     /gene="vanS-B"
                     /locus_tag="ACHA25_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8762178.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(30472..31773)
                     /locus_tag="ACHA25_000858"
     CDS             complement(30472..31773)
                     /locus_tag="ACHA25_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8762179.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            32052..32858
                     /locus_tag="ACHA25_000859"
     CDS             32052..32858
                     /locus_tag="ACHA25_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8762180.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            32876..33583
                     /locus_tag="ACHA25_000860"
     CDS             32876..33583
                     /locus_tag="ACHA25_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8762181.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            33580..34551
                     /locus_tag="ACHA25_000861"
     CDS             33580..34551
                     /locus_tag="ACHA25_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8762182.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            34544..35572
                     /gene="vanB"
                     /locus_tag="ACHA25_000862"
     CDS             34544..35572
                     /gene="vanB"
                     /locus_tag="ACHA25_000862"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8762183.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            35578..36186
                     /locus_tag="ACHA25_000863"
     CDS             35578..36186
                     /locus_tag="ACHA25_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8762184.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(36252..36425)
                     /locus_tag="ACHA25_000864"
     CDS             complement(36252..36425)
                     /locus_tag="ACHA25_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762185.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         9803 a         8767 c         9417 g         8532 t
ORIGIN      
        1 aatcagttct acgcgtacga ttattaaaaa taataaattt tcactagaag aaaatggaaa
       61 taaaatcaaa taattaggaa gaaaaaacaa agcaattaga aaagcctgca agcaaatttg
      121 agtaatcgag atccaatttt tttaaggaag tttaacaaaa atatgtaatt ctattatcac
      181 tattaaagag aaagttggag cggaatataa agaaaagaga aaagtaatta aacgcaaaaa
      241 aatgtagaac ttaaaaaata taaagaggaa caaccgttaa aattaggaat gatgttgtgt
      301 aaacattata attgctatga tataaatgaa gtgttaaaga aagcaaattt aaaccaattt
      361 tgtgaacaga aaagccttag tcactatgac caaaactacg gacgaaacta tgaccaaaag
      421 taggtactaa attatgaccg aaaataggtc caaaatggat ttttttatga tatcaaaaat
      481 tatgaaatga ttttgaaaga aaaccagttg gcacagccaa cttagccacc ttcctgtagg
      541 aaggtgctat cagttttccc ttatgctctt ttctttcttt tttgtttcat atgtcaaatg
      601 ccattttgaa atgtaccatc ttaagaaatt tatccttaaa aacaattgcg ttattggata
      661 aaaagatgct acattatctt aaaaaaggaa tcaaatatga tgaatttagt ggctagcata
      721 tcgtgtgatt aagatacttt atagtgtaag ttgaaggaga aaaaatattt aaaatatact
      781 tcccatttca gtgggaagta aaattaatct tgttgtttct actagtaatg tatctttttg
      841 gaaactatgt aacgatgtaa acaagatttt tttcagaaga aaaaacgata aaagttgtga
      901 taaacgattt aaaaaatagg agaaaatata tcaagcgcat ggtatgaaac gcacatttaa
      961 aaagattcga ctaactaaaa aaataagttt acaaagcatt gttgtgtatt acaagtgatc
     1021 tatacactga aaaatgatag agaaaatctg atacgtccgc aacaatggac aattgaaaat
     1081 gaaagtgagg aaattggaag aatggctgat gaaaataaag ttgttccgct tgaaacacta
     1141 gtgataaata aggaagagtt tgagcaactt tttcttgatt tttttgttca atatgacagg
     1201 atttatgatt ttagttcgtc actagattta tttgaattag aggcagaaat aatagaaaac
     1261 agtcgagagg agaatggtaa aatcatcatt tcagtagaaa aattcaatga attattctca
     1321 gaaacatttg aaatagatac agattattat caaccacaag aagttttcca atcatttaga
     1381 aatcagatta ttcgctcagg taagaccgta aactgaaaaa caataaaaga aacgttgaaa
     1441 gaataattat tccttcaacg tttctttttt agaatgtttt aatgaccaaa gcagtttcat
     1501 tgtcaggctt aggtaattca tgaaatcccg tatttatagt gctcaattga tcaactaacc
     1561 atgttttact ttttgaattg tagataaatc taaaattaat atcgttatta tcgtgttgaa
     1621 ggggcgtctc caaataatct gctgtatatt catagtcaac gtaacctgct aatacaaaag
     1681 agagtatccc tttatttaat gttagattta atggataatc atacgacttt atttttttca
     1741 acttcatatt gtacatatct tcagccataa aagttgagtt cgtgatttca tcttgggtct
     1801 cattaaaaaa gttatccgaa taatcgctta tttttgaaaa gtctttattg gcaccagcct
     1861 caaaaaattg ttcaactttt tcagtataaa gttgtgctaa cgctcctttt tcaatattaa
     1921 ctttaatatt ttcatgtcta tcaatttgtg tgctgttcag taagggaaga tcagctttta
     1981 gttttatatc gtcttgattt ctttcaattt caattggcaa aatcttttca gtagattcag
     2041 tttctgataa aggaacatct agttttttat catgataata agcttcaaat ccatctggaa
     2101 ccggaaaaaa atgtattggg taattctttt ctgaggttgt tgtactagtt tccgtacttg
     2161 tagaacttgt gtcagaagtg gtacacccgc ttatagttcc gatgagtaaa gcgcaagtta
     2221 gaatgattgc aaatttcttc aaaaaaattc ctccttaaaa ttgaatatgg actaatacta
     2281 gtcccattgt agcataccga atctttaaga ttaataagaa aaaggactaa ttttagtcca
     2341 aaaggtgtgc tattcttgaa agaggaaaaa attattgata aagaaaatgt aatcggtagc
     2401 aatattagac gagtccgatt agaaaaagga attggtcaaa ccgagctgat tagagaccta
     2461 cagcttcgta aaattgctat aactagggaa acgttggtta agattgaagg tggccgccaa
     2521 cacattaagt tggatcaatt gaaagccatc aaagatattc tacaggtgtc ttatgaagat
     2581 ttgcttcaat gactacttgt agtcatttcg tttgaaatta ttatgttaca cccttatgtg
     2641 tcacgaagaa atactggtat aataatgaat gggatatgta acgatagggt atactgttat
     2701 gttgcctaat aaattgtgga tcaaaataca agaatggagg gatatccttg tataaggcat
     2761 atgaattgga ttttacaaat gctgacttaa atgcattctc ctcaagctcg caatcatata
     2821 cacatgtagt aaatcaaata gatcacaacc aaaaatctat taacaagaga atagaaaatc
     2881 tatttgaaag tgaaaatgat ttagttgatt attattctaa ggacagcaaa attctagatg
     2941 cagacgaaat tattaaagat tggtttccaa ctataaaagc agatattttt atatctcact
     3001 ctcatgctga tgaaaaatta gctatacgtt ttgcatcatg gctatttgaa aattttggtc
     3061 ttactgcatt tattgattct tcggtttggg gatacagttc tgatttatta aaaaaaatag
     3121 accaaaaata ttgctataag gaacaaacaa aaacatatga ttatgataaa agaaatgtaa
     3181 caactagtca tgttcatatg atgctttcta cagctttaaa taatatgatt gatagtacag
     3241 aatgtctgtt ttttcttaat acacctaatt caattagttt gagcaatgaa atcactaatg
     3301 aacagaaatt tacgtattcg ccatggctat atagcgaact gacgacagct tcaatagtgg
     3361 aaaaaaagaa tccacgattg gaaagtaatc ctcaaatgag caccgaggat gttagaagca
     3421 ttataaagca ctattcttcg caagaaaatc tgcaaataaa atattcttta aatttaagtg
     3481 aattaactta tttacgaaaa gctgatttga aaaaatgggc tttagatgct gtggaatgca
     3541 ctaaaggaag taatacatta gatattttat ataaaaacac cattttcact aatctctaat
     3601 caatttactg aatcgaaagg aaccgggtag aatggataag aaagttttaa taaagaggtt
     3661 ctctgaagct attgaaaaag gaaatgcagc aatatttgca ggtgcaggat tatcgatgtc
     3721 acaaggatat gtaagttggc cggaactact taatgatcct gcaacagaaa tcgggttgga
     3781 ttctaaaaaa gaaacagact tagttactct ggctcaatat tataaaaatg aaaatggtgg
     3841 atcaagagga attttgaatc agatacttat ggataatttt ggtgaagaat tagaaatatc
     3901 tgaaaatcat cgaatactag caagtttacc tattgaaact tattggacaa ctaattatga
     3961 tcatctaata gaaaaatcaa tccgagaagc ttataaaaac cctcaggtta agaaaaatta
     4021 tactcagtta gcaacaacta atccaaatgt ggatacaatt gtttataaaa tgcatggtga
     4081 tatagatgat gtcagttcga cagtaataac aagagatgat tatgaaaaat atgatgattc
     4141 gtacgcgtta tttaaagaaa ctttaaaagg agatttgtta acaaaaactt ttctcttttt
     4201 aggatttagt tttacagacc ctaatttaga aagaatttta tcagatataa gatgggtttt
     4261 gagagaaaac caaagacctc attattgtat aatgagaaaa atattataga aaaaatttta
     4321 ggttatcgct gaaagccccg gaaatacggg catttcgcag gatatgagaa ctcaatttac
     4381 taccaattta ctactttcgg attgagccgc atatatgctg aaccatactg ctaatacccg
     4441 ccaccccaaa tacggcacat cataaattga atacgacacc gtaaacgcgg cgttcctctt
     4501 tcccgtcatg ggagaacagg acgccgcttt tttatgccca aatagagaaa ggaggcgacg
     4561 ccatgctatt tacttccggc cctgaccggg cgtttgaacg gctgatgcag gaaaagcccg
     4621 gtttcgatca ctacgacggc ggcggggcgg acgcgccgga ggattgcggc acctgccgat
     4681 tttaccgccc ggattggaaa tatcaattct gccagtttgc agagtgtccc tatcagccgg
     4741 gcaagctgac cgactatgca gcggtcaaat tcacagtgaa aggagcgaac aacgctatgg
     4801 cagttttccg tgtggaaaag aacaaaggct atacggtgat gtcaaatcac cacctgcgga
     4861 ataagaacct gaccttaaag gccaagggcc tgctgtcgca gatgttgtcc ttgccggagg
     4921 attgggacta taccctaaaa ggactgtcct atatcaaccg ggaaagcatc gacgcgatca
     4981 ggacagcggt atgggagctt gaaaaagctg gatatatccg gcgtgagcag ggccgtgacg
     5041 caaaaggcaa aatggccgat atggtctata ccatctacga acagccggta ttggaaaacc
     5101 cggtattgga aaatccaaca tcggataacc cggtgttgga aaatccgaca tcggataatc
     5161 cggcgtcgga aaatccaacg caattaaata aagaagtaca aagaactgac ttatcaaaaa
     5221 aagaaaaaat aattacagat ggacaaagta cccattccat tcctatccca tccccgaccc
     5281 ctgcccctat ggagggcgag gcagcggcta cgccgccgga acggaaaagg acaggaaagg
     5341 atgaggctgt acggatttat cgggaaatca ttttggagaa catcgagtat gcctatctca
     5401 ttcaggacag gagcattgac cgtgaacagc ttgacgaaat cgtagacctg atgctggaaa
     5461 ccgtctgcac cgcccgaaag acaatccgtg tcgccgggga cgactaccct gccgagctgg
     5521 tgaaatccaa gtacatgaag ctgaccggcg aacatatccg ctttgtcctt gactgtctgc
     5581 gggagaacac aacgaaagtc cgcaatatca aacaatacct gcgggctatg ctgttcaacg
     5641 cccccagcac catttccaac tactacacgg cccttgtcgc gcacgacatg gcacagcccg
     5701 attggggaaa gcctaaatcc ggcctgccgg actattcctg cggcgagggc gaaagcctgt
     5761 aacgagaaaa ggaggacgac cctatgaagc agggcgcatt gatttttgac gagcgaacag
     5821 accgttacga catccgcttt gatctggccg actattacgg cggcctgcat tgcggggagt
     5881 gtttcgacgt gatgatcggc ggcaaatgga ggcccacccg cattgaaatg gccgaaaact
     5941 ggtatcttgt cggcatccgg gccgacgacc tttccggcct gcgggtgcgg atttgaccta
     6001 tatctacctg cggcgtctgg tggttccgcc ttagacgccg cttttgactt tctgacaagg
     6061 agggatgcga ttgcaggaag aagttactca aaagaccatt gccctgtcca tgaaaacggg
     6121 caagctcacg gctcaagtgc tgcaagcggc gctgaaaaag tacctgcaac accgggccaa
     6181 gggcaaaaac acgctgcacc acggccagca gagcctaaag cagcttaaaa agcatggggc
     6241 tgatctctcc aacattgaga tcaccgaggc caacattgga gcgttcaagc catgcgccaa
     6301 gaaatacggc gtggatttta ccttacgaaa agataagacc acccagccgc cccactacgt
     6361 tgtgattttc aaggccaagg atgcagataa ccttgatctg gcgtttcggg agtttaccgc
     6421 caagacgctt tccaaggagc agcggccctc tatccgcaag gttttgtccg ctgtgaagca
     6481 aaaggcggcg gcccagacca agcagcgggc caaggagaaa atcaaggaaa gggggctgga
     6541 actgtgaagc ctgacgtaaa gaaattgctg atcctgaacc tcccgtatct gatttttgtg
     6601 tacctgtttg cgaaatgcgg ggaggcgtac cggctggccg ctggtgctga cctgtcgggg
     6661 aagctcctgc attttgcgga tggttttgcg gcggcttttg ccaaccctct gccaagcctg
     6721 cacctgtttg acctgtgtat cggtatcgcc ggtgccgtga tcgtgcggct ggtggtgtac
     6781 tgcaagagca agaacgtgaa gaaataccgc aaaggcatgg agtatggcag cgcccgctgg
     6841 gggacggcca aagacatagc gccttacatc gaccccaagt ttgaaaataa tatcctgctc
     6901 acccaaacgg aacggcttac catgaccggc aggcccaaag accccaagac agcgcggaat
     6961 aaaaacgtgc tggtgatcgg tggcagcggt tcagggaaaa cgagattttt cgtgaaaccc
     7021 aacctgatgc aatgctttcc gaccacggac tatccgactt cgtttgtggt gacagatccg
     7081 aaaggcactt tggttctgga aaccggcagg atgttccagc aggcgggcta ccgcattaaa
     7141 atcctgaata ccattaactt ttccaagtcc atgaagtaca acccctttgt ctacatccat
     7201 tcggaaaagg acgtgctaaa gctggtaaat accctgattt ccaacaccaa gggcgagggg
     7261 gaaaagtcag cggaggattt ttgggtgaag tcggaacggt tgttttattc ggccctgatc
     7321 ggctatatct ggtacgaggc cccagcggag gaaatgaact tctcaacgct gctggaaatg
     7381 ataaacgcca gtgaagcccg cgaggacgac ccggagtttc aaagccccgt cgatctcatg
     7441 tttgagcgtt tggagcagaa agaccccgac cattttgcgg tgcgccagta caagaaattc
     7501 ctgctgtccg caggcaagac ccgttcttcc atcctgattt catgcggtgc cagacttgcg
     7561 ccctttgaca tccgggaggt acgcgagctg atggaggacg acgaaatgga gctggacacc
     7621 atcggggacg aaaagacggt gctgtatctc attatgagcg atacggacac gacctttaat
     7681 ttcattctgg ctatgctcca aagccagcta atcaacctgc tttgtgaccg ggctgatgat
     7741 gtgtacggcg ggcggctgcc tgtccatgta cgcctgattt tggatgaatt tgccaacatt
     7801 ggccaaatac ccaatttcga taaactcatt gccaccattc gaagccgtga aatatcggcc
     7861 tctattattt tgcagagcca gtcgcagcta aaagcgattt acaaggacgc cgccgagatc
     7921 atttcggata actgcgatag tgtgcttttc cttagtgggc gcggcaaaaa tgccaaggag
     7981 atttccgatg cgctggggaa agaaacgatt gacagtttca acaccagcga gaaccgggga
     8041 agccaaacgt cccacggatt gaactatcaa aaattaggaa aggagttgat gtcccaagac
     8101 gaaattgccg tgatggacgg cggcaagtgt atcttgcagc tacggggtgt gcgcccgttt
     8161 ttatcggaga aattcgatat taccaagcat ccccggtaca aataccttgc cgacgccgac
     8221 aagaagaata cctttgacgt ggacagattt ttgactatca cgcgccggaa acggcagcaa
     8281 gtggtaacgc aggatgaagt attcgacctg tatgaaatcg acctgtcgga cgaggacgcc
     8341 gccgctgaat aaacagcggc ttttttcatt ccaacaacaa aatcaaaaaa tcattatgag
     8401 ccggaaaccg gcagaaagag gtaaatttat ggcatttttt aattccgcag ttactacttt
     8461 gcagactatc gtgatcggac tgggcggcgc tctgtgcgta tggggcggca tcaatctgtt
     8521 ggagggatac gggcaggaca atccgggggc aaaatcgcag ggtgtcaagc agcttgtcgc
     8581 tggcggcggc gtggccctga tcggtgtcac tctggttccc ctgctgtccg ggctgctggg
     8641 ctgatcctgg cctttgtccc tgtccaaacc cttaactaat cgcctccgcg tcctcccggc
     8701 cagaccggga gggcgcggga aaggaggtgc tacccgtgat tagtgaaatc attgaagaat
     8761 ggataaaagg catcctcatg gacggtatca cgggcaacct gtccgggctg ttcgatacgg
     8821 taaatgccaa agtcggagaa atcgccgccg acgtgggacg aaccccgcag gcatggaaca
     8881 gcggtatttt caatatgctg cacagcctgt cggaaaccgt gatcgtcccc atagccgggg
     8941 ccatactcgc ccttgtcatg tgctatgagc ttatccagat gataacggaa aagaacaata
     9001 tgcacgactt tgacagctcc atgttcttcc gctggatatt caaatccgcg tttgcaatcc
     9061 tgatcgtaac gaacacatgg aacatcgtca tgggcgtatt cgacgccacc caaagcgtcg
     9121 tcaatcaaag cgccggtgtg attatcgggc agacatccat tgattttaac accctgatac
     9181 cagaccttga aagccaattg gaggctatga gcgtgggcgc tcttttgggg ctgtggttcc
     9241 aaacccttgt tgtgggcctc accatgaata tcctttccat ctgcattttc cttgtgacct
     9301 atggccgcat ggtggaaatc tatgttgtca cgtcgctggg gcctatcccc cttgccacaa
     9361 tgggcaacgc ggaatggcgc ggcatgggcc aagggtatct gaaatccctg ctggcgctgg
     9421 gcttccaagc ctttttaatc atggttgtgg tgggtatcta cgcggtgctg atacagaata
     9481 tcggttccgc ttccgacata tccggcgcaa tctggggctg tatgggctat acggtgctgc
     9541 tgtgcttctg cctgttcaag accggcagta tatcaaaggc cgtattcggg gcgcattgat
     9601 cccaaaatcc aagcatacac aggaggaact tgtttatttc aacgtacaac atcatttacg
     9661 ctgacccgcc gtggcggtat gactgcaaaa acgtacaggg cgcagcggaa aatcactatt
     9721 ccaccatgag cattgacgag ctgtgcgccc tgccggtgga acggctggcg tccaaagact
     9781 gcctgctgtt tttgtgggcc acattcccca tgctgccgga agctctgcgg ttaatcaaag
     9841 cgtggggctt ttcattcaag accgtggctt ttgtatggct gaaacagaac cgtaaaagcc
     9901 ttacatggtt ttacgggctg ggccgctgga caaggggaaa cgcggaaatc tgcctgctgg
     9961 caacacacgg caagccgaag cgtcgttctg cctctgtcca tcagtttatt atcagcccga
    10021 ttgaacaaca cagcaaaaag ccggatgtga cccgtgaaaa aatcgtagag ctggcgggcg
    10081 atttgccccg cgtggagctg tttgccagac agaaaacccc cggctgggac gtgtggggga
    10141 acgaggtgga atgtgacctt atcctgacgt gaaaggaggt gccaaatggc ttatgtaacc
    10201 gtccccaagg acttaaccca tgtgaaaagc aaggttgtgt ttgggcttac caaacggcaa
    10261 ctgatctgtt tttccggggc gctggtgatc ggcgtcccct tatattttct cacccgtgac
    10321 tatcttacca acagcgcggc ggccctgctc atggtatttg ccatgctgcc gggctttttg
    10381 cttgccatgt acgagcgtca cggccagccc cttgaagtgg tgattatgca gatcatccag
    10441 tgctgcttca tccagccaaa ggagcgtccc tatcagaccg acaacaccta caccgccctt
    10501 gtgcggcaat accagatgga acaggaggta aaggccattg tccaaaaagg aaatcccaag
    10561 agaaaccgaa aaaacaaagc tgacccgcgc ccagcgaaaa gaaattgacg cggtaatccg
    10621 taaatataag ggcgacggca ggccccacac ggcgcagcag agcatcccct acgaggtcat
    10681 gtacccggac ggcgtgtgcc gggtatcgcc cggcgtcttt tccaagtgca ttgagtttgc
    10741 ggacatcagc tatcagcttg cccagccgga cacgcagacg gctatctttg aaaaactgtg
    10801 cgacctgtat aactatgtgg acgcctccat tcatatccag ttttctttcc tcaatcggaa
    10861 agttgacccg gtgcagtacg ccaagagctt tgagatcgcg ccgcaggggg acgacttcga
    10921 cgacatccgg gccgagtaca ccggcattct gcaaaagcag cttgcaaacg gcaacaacgg
    10981 catggtaaag accaaatacc tgacattcac catcgaggcc gaaagcgtga aagcggcgcg
    11041 agccagattg aagcgcatcg gctttgacct gttaggctat ttcaagagca tgggggccgt
    11101 agcccatgtg atggacggct gggaacggct gaacctgctg catggcgtgt accacccgga
    11161 cggcgagata ttcaattttg actggaagtg gctggccccg tccggcctct ccaccaagga
    11221 ttttattgcc ccgtcctccc tgtgcttcgg caatgcaaag acattcggca tgggcggcaa
    11281 atacggcgcg gtaagtttcc tgcaaatcct gtccccggag ctgtcggacg atatgctggc
    11341 cgacttcctc aatacggaaa gcggtgtttt agtcaatctc catgtgcagg ccattgaaca
    11401 gaccaaggcg atcaagacca tcaagcgcaa aattaccgac cttgacgcta tgaagattgc
    11461 ggagcagaaa aaggccgtcc gcagcggtta cgatatggac attcttccca gcgaccttgc
    11521 cacctacggc gaggacgcca agaagctgct gacaaagttg cagacccgca atgaacggct
    11581 gttccagttg acgttccttg tcctcaacgt ggccgacacc aagcaaaagc tgaataacga
    11641 cgtgttccaa gccgcaggcg tggcccaaaa gcataactgt ccccttgtcc ggctggacta
    11701 ccagcaggaa cagggccttg caagcagcct gccgctgggc gtcaatcaga tcaagataca
    11761 gcgcagcctt acgacttcca gcgtggccgt gttcgtcccc tttgtgacgc aggagctttt
    11821 tcagggcggc gcggccatgt attacggcat caacgccaag tcccgcaaca tgattatgct
    11881 ggacaggaag caggcccgtt gccccaatgc cttgaagctc ggcacccccg gcagcggcaa
    11941 atccatgagc tgtaaatccg agatcgtcag tgtgttctta acgacgcctg acgatatttt
    12001 tatttcagac ccggaggccg aatattaccc ccttgtcaaa cggctccacg gacaggtaat
    12061 ccggctgtcg cctaccagca aggactttgt gaacccgctg gacatcaacc tgaattattc
    12121 ggaggacgat aacccgctgg ccctgaaatc cgactttgtg ctgtcgttct gtgagcttgt
    12181 catgggcggc aagaacgggc tggaagccat tgaaaagacc gtgatagacc gggctgtgcg
    12241 cgtgatctac cggccctatc tggcagaccc ccgcccggag aatatgccga tcctgtccga
    12301 cctccacaaa gcgttgttag atcagcatgt cccggaggct gaccgggtgg cgcaggccct
    12361 tgacctgtat gtgtccggct ccctgaacgt atttaaccac cgtaccaacg tggacatcgg
    12421 caaccggctt gtgtcctttg acattaagga gctgggcaag cagctcaaaa agctggggat
    12481 gctcattgtc caagaccaga tatgggggcg cgtcacggcc aaccgaagcc aaggaaaggc
    12541 cacatggtat tttgccgacg agttccattt gcttttgaaa gaggaacaga cggcggccta
    12601 ttctgcggag atttggaagc ggtttaggaa atggggcggc attccaacag gtgccaccca
    12661 gaacgtcaaa gacctgcttt cctccccgga aattgaaaat atccttgaaa acagcgactt
    12721 cattacgctg ctcaatcagg cgtccgggga tagaaaaatc cttgcggaac gcttgaattt
    12781 gtccaccgaa cagcagaagt acatcgacaa ttccgaaccc ggcgagggcc ttttgatctt
    12841 tgaaaacgtg gtgctgccct ttaccaaccc catcccgcac aacacccagc tttacaagat
    12901 tatgacgacc cgcttaaacg aggtggcggg agtatgaccc ggcgacgcca caaaggaggt
    12961 gaaccaccat gaaagagctg aaagccaagg cgaaagtgac gcaggccatg acccgcgacg
    13021 ggctgacgct ggaaaatcag gccaccggcg aggtggagaa catttccagc cgggaggccg
    13081 aacaggattt gtccgcgccg gggccgggcg gcaccgccga taagctgctg gaacgggccg
    13141 acgccatcca tgacggccat aaggccaaga aagccgcgaa gcaggccggg gctgtggccg
    13201 ccgaggggga cgctgccctg caccggccat ccgcccggct ccaactgtcg gaggaagaac
    13261 gggccgaccc ggatttacag ccctatatcc gaaaatcgga caaacgggcc gataagctgg
    13321 ataaggcccg cgccgccatc cccaagaagc gcgtcccggt caaggaaacc gtctttgacg
    13381 aggcaagcgg caaagcaaag accgtcctgc actttgacaa acaggacaag gggcctccct
    13441 ccctgaaacc caatccggca ggccgtcctg tttccgaggt ttttctattc ggccacggga
    13501 aaatccatga agtcgagcat gaaaacgtgg gtgtggaggg cggccataag ggggaggaac
    13561 ttgccgagcg gcaggccgga aaagccatcc gcagcaccat ccggcaccac aggctgaaac
    13621 cctactggga cgcggaaaag gccgagcgca ggctattgtc tgccaacgcg gagtatttct
    13681 accagaaatc tttgcgggat aacccggaga tcgcacaggc ggccagcaac cccatttccc
    13741 ggttctggca gaaacagcag atcaagcgcc agtacgccaa ggccgcccgg acagcgggcc
    13801 agaccgcagg cgcggcggca cagggcgcgg ccaagacagc cggggcctcc acgaaagcgg
    13861 ccaaggccgc agccaaagaa agtgaacggg cggcctcctt tgtggcccgc cactggaaag
    13921 gggcgctggc cgtccttgtg ttggggcttt tgatgctgct tatcatcggc ggcttacagt
    13981 cctgcaccgc catgttcggc agcgccggaa gcggcatcac ggcgtcctct tacttttccg
    14041 aggatagcga tatgttggcc gccgaggacg cctacgcgga gctggaagcc gagctgcaag
    14101 actatctgga caactacgag gccacccatg actatgacga ataccatttt gaccttgacg
    14161 agatcagcca tgacccctat gtgctgattt ccattctgtc ggcgctccat gacgggatat
    14221 ttaccatcgg ggaggtacag ggtgatttgt ccatgctctt tgacaagcaa tatatcctga
    14281 cagaaaacgt cgtggtagaa acccgttacc gcaccgaaac cgatacatgg acggacgcgg
    14341 agggcaacac ccatacagac acctaccaag tgccgtatga ctattacatc tgctacgtca
    14401 cgctggaaaa cttcgacctg tcccatgtgc cagtctatat catggacgag gaaacgctaa
    14461 gtttctattc cctgtatatg tccacgctag gaaaccggcc tgatctgttt tccggcagcc
    14521 agtaccccaa cgcctccacc ctgacgccgc cgacctacta cgacgtaccc ccggaggctt
    14581 tggaggatgc cacctttgcc gccatgctca aagaggcgga aaaatatatc ggttatccct
    14641 atgtgtgggg cggcagctct cccagcacca gctttgattg cagcggcttc atttcgtggg
    14701 taatcaatca tagtggatgg aacgtgggac ggctgggggc gcagggccta tataacatct
    14761 gcacccctgt ttccagttcg caggccaagc ccggcgacct tgtatttttc aagggaacct
    14821 acgacacccc cggcgtgtcc cacgtcggcc tgtatgtggg caacaatgtg atgctgcatt
    14881 gcgggaaccc gatttcctac acgaacctta cttcaaacta ctggcaatcc catttttaca
    14941 gttacgggcg tttaccttaa cgccagaaag gagctttcaa ttatggcaac caaacttgac
    15001 cgcatcgaaa aggacatcca gaaaaccaag tctaagatcg cggagtttca gaaacagctt
    15061 cgggagctgg aaacgcagaa aaccgaacag gagaatttgc agatcatcca gcttgtgcgc
    15121 ggcatgaaca tgaagccgga ggaatttgcc gcgttcttac gcagcggcgc aatgcaggcc
    15181 gcccctgccg caacccctta ccatgaacag gaggacaatg cccatgaaga ataagaccag
    15241 acggatttcc cgtattctcc ccgcacttct ggccgtgatg ctctgcgtca cggcctttcc
    15301 cgttaccgcc cttgcaggcg gcaatgaccc ggcaccggcc ccgctgccgg aggctacaac
    15361 cgaacccgct acgggcggtt tggagcctga aacagacgaa accggcaccg ccacggacgg
    15421 gacagaaacc gaggccgaaa aagataccgg ccctctgacc ggcaaggaca tcaccgacct
    15481 gttttccgcg ctgttcggtt ccaaggtgag cattgccgcc acggacgacg gcatccagat
    15541 caccaccggc accgacaaaa aagaacctga acagaccggc accgtcacca ccaacggcgg
    15601 caggctgaat gtccgcaccg gcgcggggct tgacaagacc gcgttcaccc agcttcccaa
    15661 tgggacgacg gttgaggtag tcggcacaga cggcgactgg attaaaattc tcttgccgga
    15721 gcgcatcggc tatgtccatt ccgactatat gaccgtcagc gaaaaggaag tagcggctac
    15781 cggcgagggc ggtttttccc tctccattga cccggaggaa attgcttctc tgctggaact
    15841 gttcaatggc ggcggggcca gcgccgccct gtccccggac gggaacctgt cactgattga
    15901 cgacatcggc agcgccgcga aaaatggcaa gcagtttatc accgtggaaa ccaaaaacgg
    15961 caatgtgttt tatctgatta ttgaccggga cgacgaggga aaagaaactg tccatttctt
    16021 aaatcaggtg gatgaagccg atctgctgac gctgatggag gacggggaaa ccgtcacccc
    16081 cgctgctgtc tgctcctgta ccacgaaatg caaggccggg gctgtgaaca cgaattgccc
    16141 ggtatgtaaa accaacctga ccgagtgcag cggcccggag ccgcaggaac cccagccgga
    16201 ggaacccgaa gccccggagg aagaacccaa gggcggcgcg ggcggcctga tcgtgttcct
    16261 gctggtggcg ctggcgggcg gcggtgcggc cctttactat ttcaagttca aaaagcccaa
    16321 ggctgaaacc accggcagcg acgatttggg cgagtatgat tttggggagg acgaggattt
    16381 ggaggacgag gaacccgaaa ccgagtttga cccggaagct gattttgtga gtgaacagga
    16441 ggacgaggaa tgacatataa acttgtaatc gcggaaaaac ccagcgtggc ccgcagcatt
    16501 gcgggcgtga tcggggccac ggagaaacac gacggctatt tggaggggaa cggctatctg
    16561 gtgagctggt gcatcggaca tctggtgtcc tttgccgacg ccggacgcta tgacgagcgt
    16621 ttcaaaaaat ggcgctatga ggatttgccc attctcccgg aaacatggca gtacatcgtc
    16681 cccgacgaca agaaacagca gtttgacgtt ctctgttcgc tgatgcagcg ccccgacgtg
    16741 accggccttg tgtgcgccac cgacgccggg cgcgaggggg aattgatttt ccgtttcgtc
    16801 taccagatgg cgggatgcca aaagcccttt gagcgccttt ggatttcctc tatggaggac
    16861 agtgctatta aggacggctt tgcccacctg aaaccgggcg gcgactatga tccgctgtat
    16921 caatcggccc tgtgccgggc caaggccgac tggctgatcg gcattaacgc cacccggctt
    16981 ttttccgtcc tgtaccacaa gaacctgact gtgggccgcg tccagacgcc gaccttaaaa
    17041 atgctggtgg acagggacgc caagattacc gatttcaaga aagagaaata tcacatcgtc
    17101 catatcacgg cgggcggcgc ggacgcggta agcagccgct tttccgacgc tgccgaggca
    17161 aacaccgtca aggcggcttg tgcaaaagcg caggccgttt gcgtttccgt gacgcgggag
    17221 aaaaagacgg aacagccgcc caagctctat gaccttacca ccttgcagcg ggaggctaac
    17281 cggcttttcg gctataccgc caagcagacc cttgactatg cccaggcact gtatctaaag
    17341 aagctgctga cctatccgag aacggatagt caatatctta ccgacgatat gctgcccgcc
    17401 gtggaaagcc ttgtgtccgg cctgtgggag ctggtgccgt ttgccaaggg cctgaacctt
    17461 tccccgcagt ttgaccgcat cctgaacagc aaaaaggtgt ccgaccacca cgccattatc
    17521 cccaccgctg aatttgtgaa gcagggcttt ggcactctgg ccgagagtga aaaaaagctg
    17581 ctgtccctga tctgctgcaa gctcttatgt gccgtggccg ggccgcatat ctatgaggcc
    17641 gtcaccgcca catttacctg tgccgggcag gagtttaccg ccaagggcaa gaccgtcctt
    17701 tcccccggct ggaaagagat tgaacgccgg ttccgttctt ccttaaagac cgacgccgac
    17761 gaggacgccg aggcggtgcg ggagctgccg gagctttgcg agggccagac ctttgcagac
    17821 gtggccgcct ctgtcaccga gcatttcacc acgccgccca agccctacac cgaagatacg
    17881 cttttgtccg ctatggaacg ggccggggcc gaggatatgc cggaggatgc cgagcgcaag
    17941 ggattaggca caccggccac ccgcgccgcc attctggaaa agctggtgca gatgggcttt
    18001 gtccagagaa aaggcaagca gctcattccc accaaggacg gcatcaatct ggctgtggtg
    18061 ctgccggagg cgcttacttc tccccagctt actgccgaat gggaaaaccg gctgaccgag
    18121 atcgccaagg ggcaggccga tcctgacgaa tttatggacg gcatcgagac gcaggcgcgg
    18181 gagctggtgc agacctattc ctgtatcagt gaggaaaagc agaaattgtt tcaggccgag
    18241 cgtgttccta tcgggacgtg tccccgctgt ggtgaggctg tctatgaggg caagaaaaac
    18301 tactactgcg gaaaccgggc ctgccagttc gtcatgtgga agaatgaccg attttttgag
    18361 gaacgcaaga aagcgttcac cccgaagatc gccgctgcgc tgctcaaaac cggcaaagtc
    18421 aaggtaaagg gcctctattc cgtaaagacc ggcaaaacct acgacgggac ggtgcttttg
    18481 gctgataccg ggggcaagta cgtcaactac cgcattgaac acaggagcta aaggacagta
    18541 acacagcaag catagggagt gtggctacac tcccggtaaa atcccccggc cgcgtccctg
    18601 ccgggccggg gatttttgct tgttggggaa gtccccaacc ccctattttt tgaaacagaa
    18661 cagagaaagg agtgaccgcg tgaacgaagt attttctatc caagtccata accggcagtt
    18721 tttggaacag ggcaaacaag gcgtctggct ggatttgccc accacaaccg aaacgctgca
    18781 agccgccctg cgggatgttg gcattactgc cgacaatccg caggattttt ttatcaacgg
    18841 gtattcctgc ccggaggaca gacaccttgc cattccctat gacatggtgc tggccgccga
    18901 cgtggacgag ctgaatttcc ttgccgcccg gctgggccag cttgacgccg ccgagatcgc
    18961 agagctgaac gccgccctgc aaaatccccg tggcggcttt gagaatatcg gccagatcat
    19021 cgactacacc gaaaatgtgg attactatgt gcatcttccc gacgttaggg gggatggaca
    19081 gttgggcgac tactacctga accgttccgg catggtggat atgccggagg aatggaaagc
    19141 cgggattgat acggcgcagt ttggacgcca cattgcccag caggaacaag gcgtttttac
    19201 ccagtacggc tatctggtga gaagcggcga cgaatggcag cgcgtccatg agggccagcc
    19261 ggtgccggag gaataccggg tgatgcacta cccccagccg gaagctgacc gggaggaacc
    19321 gcaggcccag cagccggagg catccgaaag ccccgacaaa gcgccgcccc cggttatccc
    19381 gatcatgctg gacggcatga acagcaaaga acgcatgaaa gtgattactg accgtctgga
    19441 aaccggcgta caggagcttt ttgaaagtag ccgctatatg gcttacttat ccgctatgtc
    19501 caagatgcac gattacagtc tgaacaatac gctgctgatc gccatgcagg gcggttcgat
    19561 tgtggcgggc tataacaggt ggcaggatga atttgaccgc catgtgaagc ggggcgagaa
    19621 aggcattaag atactggccc cggccccgtt caaggttaag cagcagatgg agaaaaccga
    19681 cccggccacc cagcagcccg tgatcggcgc ggacggcaag cccgtgaccg aagaacagga
    19741 agtgacggtt ccggccttta aggtggtgac agtctttgac atcagccaga ccgagggcaa
    19801 ggattttccc atgccctata tcagtgaact taccggcgac gtgcagcagt atcaggattt
    19861 ctttgccgcg ctggaacgga catcacccta tcccataagc attgagccga tttcagacgg
    19921 ggccaagggc cgctgcttct acgcaaaaca gcgcattgcc atcaacgagg gcatgagcga
    19981 attgcagacc ctaaagaccg ccatccatga gattgcccat gccaagctcc acgccattga
    20041 ccgggaggcc cccgccgagg aacaggcggc ccggccagac cgcagcaccc gcgaggtaca
    20101 ggccgaaagc gtggcctata ccgtctgcca gtattacggg ctggacacgt cggactattc
    20161 ttttggctat gtggccgatt ggagcagcgg cagggagtta tccgagctga aagcctccct
    20221 tgaaaccatc cgggccacgg ccagcgaatt gataaccgag atcgacggcc attttgccca
    20281 gcttcagcag gagcgcgagg cccggcagga acaggaggcc gtcacggttg acgttacccc
    20341 ggaggcagcg gccccggcgc aggccgagga agaacaggcc ccggcccttg atccggcagc
    20401 cgagccgctt gtgactgtgc tatggagcga aagcccccag ctaaaggacg gccagcaaat
    20461 gccgttgcat gaggccgacg cgctgtttgc ccgtctggat gcagcatatc cggcagaggc
    20521 aggctatgac aaaaccgcgt tccgcattga ctgtatcttt cagggcgagc cggacagcta
    20581 cgaggggcgg caggattttg gagataggga cggttccctg atcgaacata tccagagcta
    20641 ccaccaattt tacgcgcagg acgaaaactg gaaaaagcat gtaatcgcta caaccggcat
    20701 agagggctgg gagcaggaca aggcccatcg ggatatgctg ttaggcgagt ttgtccccta
    20761 tatgaagctg cattgcagcc ttgcccaaat ggaacagacg gcgcagggcc gcttacagtc
    20821 cggggaaacc ctgacgccgg aggaaacggc ctatttgggg gcgcttctct cttatgtgca
    20881 ggagtgccgc cccatgctca atcagggcca gtatcagttg ccggagccgc cgaaactggc
    20941 tgacttcgac cagagcttgc aggactacaa agcacaggta caggccgaga tcgcgcagga
    21001 ggccgccgca gccggtatga ccgtggagga atacgcggcg gctggctatg aagcacccca
    21061 acaggacacc ttttccatct accagcttcg aggcggggag gaaacccgcg actatcggtt
    21121 tgagccatac gagcgtttac aggccgcagg gctggccgtg gacagtgcca actatgacct
    21181 tgtttatacg gcaccgctgg ataaaagcac gtctttggag gatatttacc gcacattcaa
    21241 catcgaccac ccggcagatt ttaggggaca cagcctttcc gtttccgacg tggtggtgct
    21301 gcaccagaat ggacaggaca ccgcccacta tgtagacagc ttcggctata tggaagtgcc
    21361 ggagtttttg cgggaaaatc ccctgcgtac cgccgaactg tctacggagc agaacgaaaa
    21421 catgattgac ggcattatca acaatacgcc gcccgaaccg cagccggagg aacccgaaca
    21481 gctatctttt gaggatgtgg cagttgcttc cggccagaca caggaggaac ccccggaggc
    21541 tgcgcccccg caaacggctg tccgctatta tcccatcaat gagggagccg cccgccgcgc
    21601 caaggaagcc atgagctttt ctgactaccg gcccggcagc gccacggcgg aataccggca
    21661 ctatgtcgat caggccgtgg aaattgcgga gcgccagaaa aagcgtgtgg agccagagta
    21721 tcacgaaaag atagacagcc tccttgacac ctacgcccgc aagctggccg agaacatgaa
    21781 taagggctat gagattaccg cccgcgtccc ctctgtcctg attgccgggc cgtccaattt
    21841 cccggtacgg gcgaaagaaa agcagaacgc cgccagtgac cgcaacatgg aggaatttca
    21901 gtacattcag ggcttgcttg acaaaatccg cagcaccggc atgggtggta tcagcgccga
    21961 cgatccgcag gccgtttcca agctggaaaa gaaactggaa aagctggaag cgtcgcagga
    22021 gttgatgaag gccgtcaatg cctactaccg caagcatggg acgctggacg gatgccccca
    22081 tctgatcgaa agaggcattg aaaacctgaa agcggatatg gcgtcgggct ggcactatga
    22141 gaaaaaaccg ttccagtcct ggcagctctc caacaataac gccgagatac gccggttaaa
    22201 gggccgcatt gaggaattga cccgccaaaa ggaggcggcc tatgtgggat gggagtttga
    22261 cggcggcact gtggagatca accgggtggc caaccgcctg caaatcttct ttgaggggaa
    22321 gccggatgct gctgtacgcg acgagctgaa aagcaacggt ttccgctggt cgcccaaggc
    22381 cggggcatgg cagcggcagt taaacgacac cacaatccgc gtcgctgacc gtatcaagtg
    22441 catccagccc ctttccggcg aaaagcccag cgccttacag cttgcggccc gcagggagaa
    22501 agaaaagccc tctatccggg cgcagctcaa cgcggccaag accgaacagg ccaagcagcc
    22561 accggcgcgg gaaaaatcaa agggattgga ggtagagtaa atgcagaagt ttgaaaatgt
    22621 ggacgttctg gccgcgctgg aacagatcat gcggcagaac accgcgtttt atcaaagtga
    22681 ctttggcatt gacaaagaca ttatcaggcg ggatgccgcc agcgatcagg ccgcagacaa
    22741 gaccctgctc tggatgtccc ggccctccgg gacgtactgt ttccgggaac gtgatgtttt
    22801 cctgaaagac acccgacagt acaacacatg gaaattttac ggggagcaga cccgtgacaa
    22861 gatactggcc tacgcggtgg agctgaccgg cacacagggc ggcactatcc ggggcaatct
    22921 ctatgagctg gactatccgc agcatttccg gcacgtcatt gaaaccgccc agccggtgag
    22981 tgtgaaccgg ctcttttatg agcatggcac ccgtgacatc ccggagagcc agcgttttga
    23041 cggaagccct gaccgggtat tgggaaactt tttgtactat gaggcccagc cccatgaccc
    23101 ggctgtgctg caggaggcgc tacggacgga gcagcacagc cgggggcggg ccgtccccgg
    23161 cgatttcaaa gtccacgtca ccgctttgca tgacagccgg attgaaaccg aggcccggcg
    23221 tattgtggac agcctgaaag atctgtccgc gcctaacagc cccaataaaa cgcatttcat
    23281 ggtggagatc tccccgcact ttgcggcgat tgcttccacc aaggataatg accggctctt
    23341 tgccatgctg ccctataaat ccctgtgttt taccgggatg aaaggcaggc atggcgtgta
    23401 tgccgtgatt aaccgggacg agcgccggga tgtgagtatt cgcaggcccc ggccctctat
    23461 ccgcaagcag ctatccgaca gcaaacaggc cgccagccca aaaaaggcgg cggccagaac
    23521 caaaaagaat gaattggagg tataaggaat gatgcacttt accgtggagg aagaaaacct
    23581 gatctgtatg taccacaatg cagaccggcg cagaacgata aacaggctca ccgccgtcat
    23641 gccggttatg gatggggata tgcgggcgca ggcccagcag accctttcca agctggaacg
    23701 catgacggat gccgactatg acggccagaa gttccacttc acagacgaat aacgaacagc
    23761 agacgccggg cgcggcggcc atgtggtcgc ttgcgtccgg cgtcttttct ttttgcctat
    23821 tcctacgcat ttagaacccg gtattgtcgg ggataggatg aagtattcgc ttgcccctgc
    23881 aaacatgcgg gagaagcctt gcagcgggcg gttttcaatc ccttaaatgc gtaggttgca
    23941 gctataatta gacccctgaa acagatatag ccgcgcagcg ggtgtatctg tttcaggggt
    24001 gtgcaggtga tagccgcaaa gcggcgtaag agggtgcagc cctcttgccg tctatctctg
    24061 ttccagcggg aagctcggca atgcttccag cagtttcagg gaaatgcgct gccgtatatc
    24121 ttcgtcaatg actttcttct ttttgccgtt tgcctgtctg atctccaccg tggaaagctc
    24181 gttgatgtag tcagcataat gggccaacac tttttccgtg gcccacctct caccggcaac
    24241 ggcggctttg atcgtttcat agtccagcag cttttcttgt ccgtccatcc tgataccccc
    24301 gtcagactga ttttttctat gataccatat tcggaagcat ggaaccagag aaaacggcgc
    24361 ttttctgctg gtaaattcta cgcatttaga acagcacttc ggcggggata ggataaaaca
    24421 cccacatacc ccgctgacca tgccggaaaa gccttgaaat ataagggctt ttacagctct
    24481 aagtgcgtag gtttccagta tcttttccgc tggcgctcgg cctctttgcg gcggcgcatc
    24541 cgtttggagc aggccgcgca gtatttggcc cggttagact tcggcgtgaa cgtggccccg
    24601 cactcacagc agcgtttcac ttcgtcccgg tctttggtga tctcggcata cagggccgca
    24661 tccagcggga ggacagcggc cttaaaccag cggcacagaa gcgaatagga aatgctctgt
    24721 acgcaaatac attcttcgcc gtcgtccagc aacaggcagt tgccgtcaca atagttgcag
    24781 cactcatgcg tcagcttccg ggccttgcga tattgagggt aattcattcg tggaatactc
    24841 atagctcttg cgtccattcc tttcccggcg tcatacgcaa aatgccgtcc acattctgct
    24901 tgatgatgcc gtattcctgc atctgctttt tcgcttcgcc gtacttctca tacagccgtt
    24961 ccttttcctc ggccaatcgc ttgtactcgg ctttcagggc gtacagatcg gggagctttt
    25021 tcacaccctg tccctttagg gcctttgccg cagcttcata caaaatcaac gtgctttcat
    25081 gctcacgccg gaattttgcc ttatccgctg cattgcggta ttccatagcc acgggccaga
    25141 gctgcttgta ggtagaaata ttctttatca gcaccgccat atccgcaagc cggtgttcca
    25201 tagctttgag ggtagcggcg gcctcgtcgc tggcggcggt gatctcggcc actttgcttt
    25261 ccaaatccgg gtaggtgtct atcccatgtt cagtcaggaa attcaaagtc tttgcggcct
    25321 gcttcaaatt gtggagcttc gcccacctct catagcctgc cgactgctgg gccttaatgt
    25381 tgttttccag atcaatccgc aaagaaatcc ccttgcgctc tttgggcgct ctggtgataa
    25441 cgcggccttt gatacgctct ttgatggctt cctcggtgta ggcttccccc aatgtcttgc
    25501 agcgggtaaa gcgttcctgt ccgggagcgc ggaaagaaat aaactttccc ggcttgatct
    25561 catagccctg cgcctgcatg agccgcagga aatcatcaaa atcttttgcc tgcgggatag
    25621 cggcgtcaat ggctgctttg agcttcgctt tccagctcgt ccctttccgc tgggcgtccc
    25681 actcggcata gctcttgccc ttactctggc cgggcttcac cacggacaat ccgttttcct
    25741 tgcacagcct gtcgctggtg cgccggatat aggcatagct ctgtttgttg gaaatgtact
    25801 tgcgctgatt taccatatcc acggcacaga aaataatgtg attgtggacg tggcccttat
    25861 caatatgggt agtcagcaca tagggatatt tcccttgcag cacttcctcg gcaagctgct
    25921 tcccgatctc atgggcctgt tccggcgtgg cttcgccggg ggcaaaggat tgtatcaggt
    25981 ggtggcccag attattgccc ttttggtatg cctgcgccag cagcatttca aattcaatgt
    26041 ccgctgtttc ataagagcag gcaaaggagg ccaccagcag cttgccgtca gttttgtccg
    26101 ggttctcgat gtagtcaagg gcttttttca gcgttgactt gataggatgt atctttgtaa
    26161 ctgccatatc tgcgccagcg ccccctttat atcctcgatg tcctgtgcgt acaccggccc
    26221 cataccgttc agcctctttg caatctgatt gacgttgacg ccgatcttct gtatctcggc
    26281 ggtctgcgcc cggatgtcgc tggtgtccat gttgacgata tagccgtcaa tgagcatctt
    26341 ccgggcgtag gctgaaaagt tcttcgtatg cagcagcgcc attttctgtt caatcatagc
    26401 ccgttcctcc ggcgtcacag ggacgcgcag cacgattttt cgctttctgt ttgccatgct
    26461 tcaccgtcct ttccattcag gggtttgggg atttccccaa caagcaaaac ggacgcggcc
    26521 cggcagggac ggggcaggcc gtttttccgg gagtggctac actccctatg cttgctacgt
    26581 tactgtttct ccactaacaa gacgtttcca aagccccaaa ctacccgcaa aatagccctc
    26641 tggaaagaaa aaagtgcaaa aaaatagaag ctacgctgtg cagctcccgc taaaagtaaa
    26701 acagaggatg ccagttacac atcctccgtt tcattcttct tcggcttttc aattagtatg
    26761 gcgctgtcaa tcgcgccctc gatgatcggc aggtattctt cggggcagag tttcagcttg
    26821 tggctgaccc tttgccgcag ttcgctttcc tcccgcatga tctccgggtt aaagtagcgt
    26881 tccacaggaa gcccgcaggc tttaataagc tgtatgacaa ccggcagact gggaatggcc
    26941 cgtcgttttc gatgttggca agatagcgcc agtcaatatt tattttctct gccattgctt
    27001 tgcgcgaaac gcgctttgct tgccgggccg ctttcacatc cgcgccgaaa gtttcaaagc
    27061 cgggacaatc ttcaatattc gccatatcgc acctcccact tacattgtat atttcatact
    27121 ctgcccgtgg aatgttggta tatgcacctt tcttaaagtt tatatttcac attttgtttc
    27181 ttttattctt cgagcaaaag aattataatg taccctgtgg aggtgtacta tggactacat
    27241 gacgttgaaa gaggcaggcg agaaatgggg tgtgccccca cgatggataa actactattg
    27301 tgctgcggga cgcatccccg gcgctgtaaa gatggcgacg atctggctca ttcccaaaac
    27361 tgcggaaaag ccgattgaca ggcgtacaaa gaaaggaaag gcggttactg aatagtataa
    27421 tgaaaccggc gcattttgga tatggttttg aagagagaga aagcatggta ttttgtcacg
    27481 gaaaaactat aagatgatag atggcaggct gctccagacc gataaaaagt attctcatct
    27541 gaaacagaaa gaaaagattt acacatggat gtatgaagaa gcgaaacttt atcatgacag
    27601 aaatggaaaa tgcccgaaac aaagacgagg aagctgctgc tgtatcagcc gtatatggta
    27661 gaattgaaaa ggccggcatc tggattcttt atggtgaagt ctccagacat tggataactg
    27721 ctggtctaat gcgcctttga gtatggggtg attgatttta ataccgggga ggtacggctt
    27781 tttgaggaaa atattctggg atttacattt ggcgattatt acatttcctg taactgcaag
    27841 gacacggatg atgaagatat gaggaatata gacttgctga ttttctactg tcttgaacgg
    27901 tattaggact gaatctgctg cgcggagtgc ggcaagagcg accggaggga gtacacacag
    27961 gccctgcctg tgtgaaaagc cataccgcaa ggtgcattcg caaaaaagag gccgcgcatt
    28021 ttgctgaggc ttggggtgtc cccaacaagc aaaacgagaa cgccgcaggc gggcaagtgg
    28081 tggatcagaa tatccgtaaa ggatattctg atccattgct tgccagtatt atcgggatgt
    28141 tttgacaacc gctgtaatcg aaacatccca aacattaaga aggtgttaat tattattatc
    28201 ttgatttaat tcgtttaaac caataatata gatacataca ccatgaaaag aagcgagtat
    28261 cccaatgacg gatgattaaa atccgaaaag aaatcgcttt gaaactacag ggaaactaca
    28321 gactgttatg ttatcttctt aaaatggagg gatttttatg tcgatacgaa ttctacttgt
    28381 cgaggatgat gatcatatct gtaatacagt aagggcgttt ctggctgagg caggatatca
    28441 ggtggatgcc tgcacagatg gaaatgaggc atacaccaag ttttacgaaa acacttatca
    28501 actggttatt cttgatatta tgctgcccgg tatgaacggg catgaacttt tgcgtgaatt
    28561 tcgtgcgaaa aatgatactc ccattctgat gatgacagcc ctgtcggatg acgaaaacca
    28621 aatccgggcg tttgatgcag aggcagacga ctatgtaaca aagccattca agatgcagat
    28681 tttactaaag cgggtggaag ccctgctgcg gcgcagcggt gcgctggcaa aggaaatccg
    28741 tgtcggcagg ctgacacttc tgccggagga tttcacggta ctttgtgacg gtacagagct
    28801 gcccctgaca cgaaaagaat ttgaaatcct tttgctgctg gtgcagaaca aaggcagaac
    28861 cttaacgcat gaaatcattt tgtcacgtat atggggatat gactttgagg gtgatggcag
    28921 cacagtccac actcatatca aaaatttgcg ggccaagctg ccggaaaata tcatcaaaac
    28981 cattcgcggt gtaggatacc gattggagga atcataataa tggaaagaaa agggattttc
    29041 attaaggttt tttcctatac gatcattgtc ctgctactgc ttgtcggtgt aacagcaaca
    29101 ctgtttgcac agcaatttgt gtcttatttc agagtgatgg aattacagca aacagtaaaa
    29161 tcctatcagc cattggtgga actgattcag aatagcgata ggcttgatat tcaagaggtg
    29221 gcagggctgt ttcactacaa taaccaatcc tttgagtttt atattgaaga taaagaggga
    29281 agcgtactct atgccacacc aaatgccaat acatcaaata gttttagacc caactttctt
    29341 tatgtggtac atagagatga taatatttcg attgttgctc aaagcaaggc aggtgtggga
    29401 ttgctttatc aagggctgac aattcgggga attgttatga ttgcgataat ggttgtattc
    29461 agccttttat gcgcgtatat ctttgcgcgg caaatgacaa cgccgatcaa agccttagcg
    29521 gacagtgcga ataaaatggc aaacctgaaa gatgtaccgc cgccgctgga gcgaaaggat
    29581 gagcttggcg cactggctca tgatatgcat tccatgtatg tcaggctgaa agaaaccatc
    29641 gcaaggctgg aggatgaaat cgcaagggaa catgagttgg aggaaacaca gcgatatttc
    29701 tttgcggcag cttctcatga gctaaaaacg cccatcgcgg ctacaagcgt tctgttggag
    29761 ggaatgcttg aaaatatcgg tgactacaaa gatcattcta agtatctgcg cgaatgcatc
    29821 aaaatgatgg ataggcaggg caaaatcatt tccgaaatac tggagcttgt cagcctgaat
    29881 gatgggagaa tcgtacccat agctgaaccg ttggacatag ggcgcacggt tgccgagttg
    29941 ctgcccgatt ttcaaacctt ggcagaggca aacaaccagc ggttcgtcac agatattcca
    30001 gccgggcaaa ttgtcctgtc cgatccgagg ctgctccaaa aggcactatc caatgtcata
    30061 ttgaatgcag ttcagaacac gccgcaggga ggcgaggtac ggatatggag tgagcctggt
    30121 gctgaaaaat gccgcctttt tgttttgaac atgggcgttc acattgatga tactgcgctt
    30181 ccaaggctgt tcaccccatt ctatcgcatt gatcaggcgc gaagcagaaa aagtgggcga
    30241 agcagaaaaa gtgggcgaag cggtttagga cttgccatcg tacaaaaaac gctggatgcc
    30301 atgagcctcc agtatgcgct ggaaaacacc tcggatggcg ttttgttctg gctggattta
    30361 ccgctcacat caacattgta aatatttaaa acttaaatga ttttgggctc tttgtcaaat
    30421 aggactgata gagtgagata agaacatcta aggtagaatt ccttttctac tttagatgtt
    30481 ttttgattca taggtaatca gattatttag taagaaaatt ctggtcttca ttttccgaaa
    30541 gcttcgaaag ccataagcga ctcgtttcaa tgctttgatg tgggtatgca gattttctat
    30601 tttggcattt gaataaggca attcgagggc taaacgaatg ccggattcat agtttataaa
    30661 agcttttttt atagaatgca gttcttttgg aacggaattg ggcatcgttc ttacaagttt
    30721 aaagaaagtg tccgcctctt tgtcgtgaaa cgcttgaaga atttcttgat agtaaaaata
    30781 ggcgattcgt aattcgtcag agatcgaaag cagacgatcc accacttcac tttcggtgag
    30841 atacgcccaa ccaaaattgc gccatttggt ccatttgtca taaacaagct tgtcctgtcg
    30901 tttgatcaga agccgagaca acgcctttaa ttgtcgataa tatttggctt gtgttggatc
    30961 gtttcgatca aagcttttca tgattcgtac gcgaatatcc tcaaatttac gaatgagatg
    31021 tttgacaatg tgaaaacgat ccacaacgag ttttgccttt ggaaaacatt cctttatcag
    31081 agaggcataa gaaacattca tgtccgtcac gatgaccttt accttcaatc gtgcgcgtcg
    31141 ttcaaattgc ataaaataag aaacgagagc ctttttcttc cgtgaaggaa gaatatccag
    31201 taattttcca gtatcgccat ccatacaaga aaaggagtag ggaccgactt gatttttcag
    31261 tgaacgaaat tcatccatta ataaacaacg cggtaaccaa tttcttttta cttttgtcat
    31321 ggggatcaga gaagcgagtg tccgagaaac ggtcgaccaa gaaacgccgc aatgcttagc
    31381 aattgtcgtc attgcttgtt tttcagttag ctcttctaaa attttatagc gcacttgttt
    31441 agcgataaag cagtttctat cgacaatgta ggtattcgcc gtaaaatagg tgtggcagcc
    31501 acgacagtgg tagcgttgtt tcgctaaagc aagataagtc agttggttcg cacatttatt
    31561 aagttgaatg agcgattttt tctttccgtt cttaacgatc atgccttcat gattcatctg
    31621 tttacacaaa ggacagcgac gaatttcttt acttaacgtc gcagaaacaa caagactctt
    31681 gatattctta atgctcttcg attccacatt tgtgatgaaa atgtttttat ctttgattcc
    31741 aagtagtagt ttgatatgat gaagatcagg catacagaac gctcctttga ttgtgttgtg
    31801 gtgattcaat tctaacggag cttctgtatg ttttgttttt tcatgctcaa aagggaccga
    31861 tagaatgatt ttctatcagt cctagaaatt atagagccga ttttgaccga caggtataac
    31921 cctgccggtc tttttttgct gctacaggaa aactacagac tggctacagg gaaagtacag
    31981 atgcgcttgc cataataaca atcgtaccag ccacaaatcg tagttttatt gcaaaggagg
    32041 cattcgatca aatggaaaaa agcaactatc attccaatgc ggatcatcac aaacggcata
    32101 tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg ttcacagtct
    32161 gcacgctgtt tttggggtgg agactggctt ccgtattgga ggcaacacag ataccgccca
    32221 tccctgcaac tcatacaggc agcagcactg acgtagtgga gaatttggag gaaaacgctc
    32281 ttgccaccgc caaagaacag ggagatgaac aggaatggag cctgatttta gtgaacaggc
    32341 agaaccccat ccccgcacag tacgatgtgg agcttgagca actatcaaat ggtgagcgga
    32401 tagatattcg gatttctccc tatcttcaag atttgtttga tgccgcaaga actgatggag
    32461 tttacccgat tgtcgcatcc ggataccgaa caacagaaaa acagcaagaa attatggatg
    32521 aaaaaattgc cgaatataag gcgaaaggct acacctctgc acaggctaaa gcggaagcag
    32581 aaacttgggt ggccgtgccg ggaacgagcg agcatcagct tggtcttgct gtggatatca
    32641 atgcggacgg aattcattca acaggcaacg aggtttatag atggctggat gaaaacagct
    32701 atcgttttgg ttttattcgt cgctacccac cagacaagac agagataacc ggtgtgagca
    32761 acgagccgtg gcattaccga tatgttggca tcgaagctgc cacagagatg tacaaccaag
    32821 gggtttgcct tgaggaatat ttaaaaccag aaaaatgaga aaaggatgtt atgttatgga
    32881 cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca atgagacggg cgcagagaaa
    32941 aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc tatgcgcaga cgatagggga
    33001 aaaatcgctt tcccatttgc tctttgaaac ggattgcgca ttatacaacc acaataccgg
    33061 atttgacatg atataccagg aaaacaaggt gttcaactta aagctggcag caaagacctt
    33121 aaacggctta ttgataagac cgggggaaac cttttctttc tggttgttcc tgcatacgcc
    33181 attgacaatt atccagcgca gaggtcacga agtaaaggag tttccagagc caaacagtga
    33241 cgagattaaa ggggtggatg caaccatttc agagggctgg attgatttaa aagtgcgaaa
    33301 cgataccgac tgcacctacc aaatatgggt gaccttagat gatgagaaaa tcatcggtca
    33361 ggtgttcgcc gacaaagagc cccaagcatt atacaaaatt acaaatggca gtatccagta
    33421 tgtccgtgaa agtggcggga tttatgaata tgcccaggtt aaacggatgc aagttgcctt
    33481 aggtaccggg gaaataatag attgcaagct gctttataca aacaaatgca aaatctgtta
    33541 tcccctcccg gaaagtgtgg atattcagga ggagaaccaa tgagaaaaag tgtgggcatt
    33601 actgtttttg gatgcgaaca ggatgaggca aatgctttcc gcgctttatc gccggatttt
    33661 catattatcc ctacgctgat tagcgatgcg atatcggcag acaacgcaaa attggccgct
    33721 ggcaatcaat gcgttagcgt aagccataag tccgaggttt ccgaggcgac aattcttgcg
    33781 ctgagaaagg tcggggtaaa atacatttct acccgcagca tcggctgcga tcacattgat
    33841 acgactgccg ccgagagaat gggaatctcg gttggcacgg ttgcgtattc gccggacagc
    33901 gttgcggatt atgctttgat gctgatgctg atggccatac ggggtgcaaa acccaccatg
    33961 cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc gggggaaaga actgggggat
    34021 atgactgtgg gagttattgg aaccggccat atcgggcaag cggtcgtcaa aaggctgcgg
    34081 ggatttggat gccatgtgct ggcctatgat aacagccgaa aaatggatgc agattatgtc
    34141 cagcttgatg agcttctaaa aaacagcgat attgttacgc tccatgtgcc gctttgtgcg
    34201 gatacccgcc atctgatcgg tcagaagcaa attggagaga tgaagcaagg cgcattttta
    34261 atcaacaccg ggcgcggagc gcttgtcgat accggggcgc tggtggaggc gttggaaagc
    34321 ggaaagctgg gtggtgcggc actggatgtg ttggagggcg aggatcagtt tgtttatacc
    34381 gattgctcgc agaaagtgct tgaccatccc tttttgtcgc agcttttaag gatgccgaat
    34441 gtgatcatca caccccatac ggcgtactac actgagcgtg tgctgcagga tactacagaa
    34501 aaaacaatca ggaattgtct caattttgaa aggagtttac agcatgaata gaataaaagt
    34561 cgcaatcatc ttcggcggtt gctcggagga acatgatgtg tcggtaaaag ccgcaataga
    34621 aattgctgcg aacattgata cggaaaaatt cgatccgcac tacatcggaa ttacaaaaaa
    34681 cggtgtatgg aagctatgca agaagccatg tacggaatgg gaagccgaca gtctccccgc
    34741 catactctcc ccggatagga aaacgcatgg gctgcttgtc atgaaagaaa gcgaatacga
    34801 aacacggcgt attgatgtgg ctttcccggt tttgcatggc aaatgcgggg aggatggtgc
    34861 gatacagggg ctgtttgtat tgtctggtat cccctatgtg ggctgtgata ttcaaagctc
    34921 cgcagcttgc atggacaaat cactggccta cattcttaca aaaaatgcgg gcatcgccgt
    34981 tcccgaattt caaatgattg ataaaggtga caagccggag gcgggtgcgc ttacctaccc
    35041 tgtctttgtg aagccggcac ggtcaggttc gtcctttggc gtaaccaaag taaacggtac
    35101 ggaagaactt aacgctgcga tagaagcggc aggacaatat gatggaaaaa tcttaattga
    35161 gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg gggaacgagg atgatttgat
    35221 tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc ttccgcatcc atcaggaaaa
    35281 cgagccggaa aaaggctcag aaaatgcgat gattacagtt cccgcagaca ttccggtcga
    35341 ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat cgggtgcttg gatgcagagg
    35401 gcttgcccgt gttgatcttt ttttgcagga ggatggcggc atcgttctaa atgaggtcaa
    35461 taccctgccc ggttttacat cgtacagccg ctacccacgt atgatggccg ccgcaggaat
    35521 cacgcttcct gcactgattg acagcctgat tacattggcg ttaaagaggt gataagtatg
    35581 gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg tccgttggga tgccaagtac
    35641 gccacatggg ataacttcac aggaaaaccg gtggatgggt atgaggtgaa tcgcatcatc
    35701 ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa tccatgcggc tgcgcttggc
    35761 tatggcttgc ttttatggga tggatatcgg ccaagaactg cggtggactg cttcctgcgt
    35821 tgggcagcgc aaccggagga caatctcaca aaagaaaaat tttaccccaa tatagagcga
    35881 gccgagttga ttacaaaggg ttatgtggcc tcacaatcca gccatagccg tggaagcgca
    35941 attgatctta cgctctacca cctggataca ggggaacttg tttcaatggg aagtaacttc
    36001 gattttatgg acgaacggtc gcatcataca gcaaaaggga taggggatgc agaggcacaa
    36061 aatcgaagat gcttgcgtaa aatcatggaa agcagcggat ttcagtctta tcgctttgaa
    36121 tggtggcact ataagttgat tgatgagcca taccccgata cctattttaa ttttgccgtt
    36181 tcataatgaa agtattggtt ttgtatcaga ttttggattt gataaaatct atgctggtca
    36241 tggacaatct tttactggaa agtggttaaa gcatcttttg taatacattg caaattatag
    36301 tattcaatct gcccagcggc agaaaagaaa aaaaccgctg ttgggcagac ctttcccacg
    36361 cccattttgt attttgctgg cggcggtttt gagaaatcaa ggccgccgtt cttttctggc
    36421 agcaaaaggt gtggcggccc ataggaggat gccgctatgc tgttttgcta ttgccatgct
    36481 ctaatccact tccaccactc aaaaaaagcc ttgcccctc
//
LOCUS       DAYYFD010000019        36236 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000019 DAYYFD010000000
VERSION     DAYYFD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.019"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHA25_000865"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHA25_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8762186.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHA25_000866"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHA25_000866"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8762187.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHA25_000867"
     CDS             complement(2298..2960)
                     /locus_tag="ACHA25_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8762188.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHA25_000868"
     CDS             3144..4790
                     /locus_tag="ACHA25_000868"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8762189.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHA25_000869"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHA25_000869"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8762190.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7524)
                     /locus_tag="ACHA25_000870"
                     /pseudo
     CDS             complement(5971..7524)
                     /locus_tag="ACHA25_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7672..8813
                     /locus_tag="ACHA25_000871"
                     /pseudo
     CDS             7672..8813
                     /locus_tag="ACHA25_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8939..10018)
                     /locus_tag="ACHA25_000872"
     CDS             complement(8939..10018)
                     /locus_tag="ACHA25_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8762191.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10121..10561)
                     /locus_tag="ACHA25_000873"
     CDS             complement(10121..10561)
                     /locus_tag="ACHA25_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8762192.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10618..11028)
                     /locus_tag="ACHA25_000874"
     CDS             complement(10618..11028)
                     /locus_tag="ACHA25_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8762193.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11208..11642)
                     /locus_tag="ACHA25_000875"
     CDS             complement(11208..11642)
                     /locus_tag="ACHA25_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8762194.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11742..12590)
                     /locus_tag="ACHA25_000876"
     CDS             complement(11742..12590)
                     /locus_tag="ACHA25_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8762195.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12979..14745
                     /locus_tag="ACHA25_000877"
     CDS             12979..14745
                     /locus_tag="ACHA25_000877"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8762196.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14847..16106
                     /locus_tag="ACHA25_000878"
     CDS             14847..16106
                     /locus_tag="ACHA25_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8762197.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16253..17554
                     /locus_tag="ACHA25_000879"
     CDS             16253..17554
                     /locus_tag="ACHA25_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762198.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17557..18408
                     /locus_tag="ACHA25_000880"
     CDS             17557..18408
                     /locus_tag="ACHA25_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8762199.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18421..19236
                     /locus_tag="ACHA25_000881"
     CDS             18421..19236
                     /locus_tag="ACHA25_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8762200.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VFLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19387..20553
                     /locus_tag="ACHA25_000882"
     CDS             19387..20553
                     /locus_tag="ACHA25_000882"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8762201.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20783..21430
                     /locus_tag="ACHA25_000883"
     CDS             20783..21430
                     /locus_tag="ACHA25_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8762202.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21514..22458
                     /locus_tag="ACHA25_000884"
     CDS             21514..22458
                     /locus_tag="ACHA25_000884"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8762203.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22593..24044
                     /locus_tag="ACHA25_000885"
     CDS             22593..24044
                     /locus_tag="ACHA25_000885"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8762204.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24065..24901
                     /locus_tag="ACHA25_000886"
     CDS             24065..24901
                     /locus_tag="ACHA25_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8762205.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24901..25560
                     /locus_tag="ACHA25_000887"
     CDS             24901..25560
                     /locus_tag="ACHA25_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8762206.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            25686..26375
                     /locus_tag="ACHA25_000888"
     CDS             25686..26375
                     /locus_tag="ACHA25_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762207.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26381..27337
                     /locus_tag="ACHA25_000889"
     CDS             26381..27337
                     /locus_tag="ACHA25_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8762208.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27381..27893
                     /locus_tag="ACHA25_000890"
     CDS             27381..27893
                     /locus_tag="ACHA25_000890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8762209.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28149..28697)
                     /locus_tag="ACHA25_000891"
     CDS             complement(28149..28697)
                     /locus_tag="ACHA25_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8762210.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28786..29967)
                     /locus_tag="ACHA25_000892"
     CDS             complement(28786..29967)
                     /locus_tag="ACHA25_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8762211.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30203..31711)
                     /locus_tag="ACHA25_000893"
     CDS             complement(30203..31711)
                     /locus_tag="ACHA25_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8762212.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31870..32061
                     /locus_tag="ACHA25_000894"
     CDS             31870..32061
                     /locus_tag="ACHA25_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762213.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32264..33181
                     /locus_tag="ACHA25_000895"
     CDS             32264..33181
                     /locus_tag="ACHA25_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8762214.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33174..34247
                     /locus_tag="ACHA25_000896"
     CDS             33174..34247
                     /locus_tag="ACHA25_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762215.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34395..34913
                     /locus_tag="ACHA25_000897"
     CDS             34395..34913
                     /locus_tag="ACHA25_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762216.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34894..35286
                     /locus_tag="ACHA25_000898"
     CDS             34894..35286
                     /locus_tag="ACHA25_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8762217.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35362..>36236
                     /locus_tag="ACHA25_000899"
     CDS             35362..>36236
                     /locus_tag="ACHA25_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8762218.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLP"
BASE COUNT        11990 a         6538 c         6959 g        10749 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt
     7501 tttaatcgct tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt
     7561 gaaaatatta ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa
     7621 tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat
     7681 tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca
     7741 gtccacacag aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat
     7801 cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa
     7861 ggacaaacag cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta
     7921 caatcagctg ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac
     7981 actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac
     8041 ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat
     8101 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga
     8161 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
     8221 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
     8281 gaaactcaac cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca
     8341 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
     8401 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
     8461 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
     8521 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
     8581 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
     8641 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
     8701 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
     8761 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
     8821 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
     8881 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
     8941 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
     9001 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
     9061 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
     9121 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
     9181 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
     9241 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
     9301 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
     9361 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
     9421 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
     9481 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
     9541 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
     9601 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
     9661 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
     9721 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
     9781 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
     9841 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
     9901 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
     9961 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
    10021 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    10081 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    10141 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    10201 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    10261 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    10321 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    10381 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    10441 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
    10501 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    10561 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    10621 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    10681 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    10741 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    10801 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    10861 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    10921 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
    10981 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc
    11041 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    11101 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    11161 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
    11221 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
    11281 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
    11341 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
    11401 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
    11461 agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
    11521 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
    11581 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
    11641 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
    11701 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
    11761 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
    11821 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
    11881 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
    11941 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
    12001 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
    12061 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
    12121 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
    12181 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
    12241 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
    12301 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
    12361 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
    12421 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
    12481 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
    12541 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
    12601 ccttaattca ttaataatac tcttatctta ttataactta cttttcacct taagttaact
    12661 ttaattaata aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac
    12721 ttctacgcca aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt
    12781 aagataaatt caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa
    12841 gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata
    12901 acatttttga aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa
    12961 aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat
    13021 gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc
    13081 aaaaaagtat atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa
    13141 aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa
    13201 gtgacgagcg aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg
    13261 gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca
    13321 aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg
    13381 gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca
    13441 aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga agactttttc
    13501 gggggagatt tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat
    13561 ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat
    13621 tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa
    13681 gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat
    13741 tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc
    13801 cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa
    13861 aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat
    13921 ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt
    13981 gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga
    14041 gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct
    14101 caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg
    14161 attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat
    14221 catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct
    14281 catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg
    14341 atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg
    14401 atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa
    14461 gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag
    14521 attatttctg aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat
    14581 ttcacacgga aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct
    14641 tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac
    14701 gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta
    14761 acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca
    14821 cgttgaatac aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag
    14881 ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag
    14941 cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag
    15001 attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag
    15061 tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg
    15121 gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc
    15181 tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga
    15241 cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata
    15301 acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag
    15361 acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga
    15421 ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg
    15481 atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt
    15541 atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag
    15601 gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat
    15661 ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc
    15721 taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt
    15781 atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc
    15841 aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag
    15901 caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag
    15961 cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg
    16021 atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca
    16081 gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg
    16141 gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg
    16201 ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca
    16261 agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat
    16321 ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct
    16381 attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg
    16441 aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat
    16501 cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc
    16561 gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat
    16621 aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac
    16681 gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca
    16741 tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt
    16801 tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt
    16861 atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa
    16921 atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc
    16981 ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac
    17041 tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa
    17101 aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc
    17161 ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc
    17221 agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt
    17281 cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc
    17341 catgatctat ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac
    17401 tgatattctt atttcctgga tctataaact tacgactgga acttctccgc agtattctat
    17461 ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt
    17521 taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct
    17581 cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc
    17641 attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca
    17701 gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt
    17761 gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc
    17821 gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc
    17881 cgcaaatcaa gtctgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg
    17941 actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca
    18001 atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac
    18061 actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt
    18121 tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct
    18181 tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat
    18241 cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct
    18301 ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta
    18361 caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc
    18421 atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg
    18481 ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg
    18541 atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa
    18601 tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa
    18661 ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg
    18721 gtaggtgtgt ttttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag
    18781 aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat
    18841 tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta
    18901 gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt
    18961 ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg
    19021 atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg
    19081 ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac
    19141 tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt
    19201 gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca
    19261 atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac
    19321 ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg
    19381 aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct
    19441 gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc
    19501 caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcatct
    19561 ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca
    19621 ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa
    19681 tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat
    19741 tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt
    19801 gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat
    19861 atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg
    19921 ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta
    19981 agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa
    20041 tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca
    20101 agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta
    20161 aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc
    20221 ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt
    20281 actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta
    20341 cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca agtccaagat
    20401 caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct
    20461 tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct
    20521 ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact
    20581 atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta
    20641 ggattgtctc taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct
    20701 ttaattttta aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt
    20761 tataacaaag gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc
    20821 tttgttttta gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc
    20881 cagtagcgaa cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc
    20941 gtctagccag acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc
    21001 agctcgtgcg atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga
    21061 attagatagt gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga
    21121 atacgaagca agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct
    21181 agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt
    21241 gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac
    21301 agattgggat ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg
    21361 caatcaaagt acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca
    21421 agatgattaa gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact
    21481 agacaaaata acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat
    21541 catatctttg ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa
    21601 ttagatgaac tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat
    21661 gaacgaaaag gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct
    21721 atgggacttc caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa
    21781 gtgcaggaaa accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa
    21841 aacttggaaa tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat
    21901 ttatcttgtc ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat
    21961 gaaacactga aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct
    22021 tattttgatt ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc
    22081 tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca
    22141 gtagtcatca aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact
    22201 gctttggcga atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt
    22261 acaggtggga ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg
    22321 ttacagattg gtaccgagct tcataaagaa gggccggaaa ttttttctag aatcataaaa
    22381 gaattaacgc agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa
    22441 ttacgcacca tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata
    22501 aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt
    22561 gggaaaataa cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag
    22621 agcttaaaga aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa
    22681 agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc
    22741 acgagctatt gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa
    22801 aaatgctcgg tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa
    22861 cacaccgagc ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc
    22921 cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct
    22981 atggcccagc aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag
    23041 ctgctgctgc agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt
    23101 ctattagaat acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga
    23161 tgccagttgg tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga
    23221 cagtttctat gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg
    23281 cgccatatca agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga
    23341 tagctttttg tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg
    23401 taaaaaaagc tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc
    23461 cactagatgg tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag
    23521 caatgatccg ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac
    23581 ccatttcttg gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt
    23641 ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc
    23701 tattcctttc accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg
    23761 atgcgataag agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg
    23821 ttgtttcttc catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc
    23881 tgactggaca accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag
    23941 gaatccaatt tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat
    24001 ttgaagcaaa tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag
    24061 aagaatgaag aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc
    24121 ttgcggtaca gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa
    24181 agaattagta gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa
    24241 tgaattagtt ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa
    24301 aatctccttt aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc
    24361 agattttgct gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc
    24421 agagactttc aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat
    24481 ccactcactt gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat
    24541 gcgtttagcg gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca
    24601 gtatttaagc gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca
    24661 gaaaatggcc gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt
    24721 caatgaaaac gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat
    24781 cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata
    24841 ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg
    24901 atgtactttc ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa
    24961 ggcttggtat acacattagg gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg
    25021 ttgcttttga cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt
    25081 tatttatcgt tctttagagg cattcccgca ctagtcctgc tgtttctgtt atattttggg
    25141 ttaccttatc aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt
    25201 gcatttattg gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac
    25261 gctggtttag cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa
    25321 gcttttcgaa tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact
    25381 tcgttagcag caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt
    25441 cggacattcg attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt
    25501 atcgggatcg aaaaagtgca acagtattta gaaaaacgtt ctatgcatca gtcaatctga
    25561 aaaatagaaa tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc
    25621 tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag
    25681 aaaacatgaa gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg
    25741 agcaaggcgg cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata
    25801 cgcttagctg cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag
    25861 tgacaaaagc agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt
    25921 ttgttttacc aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg
    25981 ttttagtgta tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga
    26041 ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct
    26101 tcatggcttc tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat
    26161 attttccaac agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat
    26221 tttctaacga acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg
    26281 agttgcaaga agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc
    26341 agatccgtcg gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag
    26401 aggaaaacaa tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa
    26461 taaaatccaa ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga
    26521 attatttcaa aaaaagcacc atcgaatcgc tgtgatccaa aatccccaag taggacaatt
    26581 caaattattc gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc
    26641 tgcacagatc ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct
    26701 tgttttcgat ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt
    26761 tgattcgaaa tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa
    26821 aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac
    26881 agatgacttt tttagtgatg actatctttt tttccacgca gacggatata ttggattttc
    26941 tgatttcacg atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc
    27001 tatccatcta acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga
    27061 cagtaacgac tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa
    27121 aaaatggatc atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt
    27181 ggctctttac cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat
    27241 gcatcatttg gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta
    27301 ttcaaaagtt caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg
    27361 aatagaaaag aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag
    27421 tggtagaaat cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc
    27481 aaaacgggca gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct
    27541 atgtattaat agtggaagat caaattgctg gaacagctgc attagtctca agcgtggatc
    27601 ccgtttacac aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc
    27661 atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac
    27721 atttgttgac gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag
    27781 ctaatgaacc gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg
    27841 aatttccttt tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa
    27901 atcatctgag caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca
    27961 agaggttgta acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc
    28021 tcaaattttt gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc
    28081 atcgagaggt cgtgacatag cttttgtcac gacctctcgg cttatcctac gagtattatt
    28141 cgatacattt atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca
    28201 tcgtttgaca cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct
    28261 tctacataaa ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg
    28321 cgcgattgca atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa
    28381 tactcatata aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc
    28441 agtttctttt taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac
    28501 tctgcaaact catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa
    28561 tcctcttgat ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt
    28621 tccggctgaa aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat
    28681 acatagcaat aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg
    28741 aaaatatttc caaagctttt aaaagtaact ttgctttttt gtatattata ccagcaagcc
    28801 agtcacaata gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg
    28861 ttttgcgaca agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc
    28921 gatagaagag aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc
    28981 agtaaaatgg aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag
    29041 tttagtcgcc atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa
    29101 ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg
    29161 gaagatagca ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt
    29221 gatcgccaca tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc
    29281 tttgttttct tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat
    29341 tgaagccaga atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga
    29401 atcgatcagt gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac
    29461 actcatggtg aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa
    29521 aacttccgat tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat
    29581 cccgttgatt ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat
    29641 atagcgtaga ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa
    29701 gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc
    29761 aaaagcaaga agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt
    29821 tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt
    29881 caatttgatt gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa
    29941 accaataata cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt
    30001 tacctgtttt gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg
    30061 aaacgcattc attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta
    30121 acgtcttagt aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc
    30181 aacatttata agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg
    30241 cgcaggtaat atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca
    30301 cagtagagtg ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga
    30361 ttggattcgt tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat
    30421 ccaaagagga atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg
    30481 acaataaagg aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg
    30541 ccgctgacgc atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt
    30601 cgattgcaat cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat
    30661 agtcatgggc agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa
    30721 tcagtttgat tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg
    30781 tttggaaata acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca
    30841 cgatcccaaa tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt
    30901 taccagtaat tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat
    30961 agttcacagc tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt
    31021 ttagatcgac tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc
    31081 atactgggaa agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat
    31141 acagttggtt caaggaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa
    31201 cgattggttt agttgtttca atatactcat tttttccaaa accatcttct gagacgattt
    31261 tttctacgtt gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc
    31321 tttctagttt attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga
    31381 cactgttgat taataagcca taaccacgta attcatcgat taaccgcaag atatccatat
    31441 cataagtaat cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc
    31501 aaatcagtat ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt
    31561 caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac tagcttccca ccaaattcta
    31621 aatataattt gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat
    31681 atttttctga atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt
    31741 ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa
    31801 aatggtttaa accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga
    31861 aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg
    31921 atttttaccg gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact
    31981 tatctaaacg aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca
    32041 agagatctgc ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat
    32101 cagatggaaa gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt
    32161 tatggagcat tgcccgctct tttttataac aattttttac agaaaatggt actattgaag
    32221 tattaataag tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac
    32281 aaatgcaaat tatgagccag acgaactaga ggagacgaat gcggatcagg atacaggaca
    32341 agaatcgtct tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt
    32401 agaagactta caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg
    32461 tttatacaat ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat
    32521 cgatcaattg ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga
    32581 acaagtagaa aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga
    32641 tcggcccacg gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga
    32701 tgaagagtgg gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga
    32761 gctagacgca aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa
    32821 agaaatcaat cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa
    32881 aatcgaggaa gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt
    32941 aggctggctt ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa
    33001 gcaagaagca agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat
    33061 cagacgctat ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa
    33121 ttatcttggc aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg
    33181 aacacgatga agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga
    33241 acacttcatt ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc
    33301 tagaggaaac aaaaatacac gaaaaagaaa aagtaactcc gacgaatacg aatactgcag
    33361 aaaacaccca tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag
    33421 atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta
    33481 tgagaaaaga tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta
    33541 tattgaatga agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt
    33601 atgaattact gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca
    33661 catggaaaac aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct
    33721 tttcctttgt attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa
    33781 acgtatcacc agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc
    33841 gacactggtc agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat
    33901 tgaatcaatt attagcccat gcaagaacac ttggttttac cgttaatcag acattactag
    33961 acaatacgaa gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat
    34021 tggatgatat ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg
    34081 aaactactta tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa
    34141 aagatcaaac agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct
    34201 ttatcaatta cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg
    34261 attaccagat gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata
    34321 attgtcacaa gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag
    34381 ggatttcatt aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt
    34441 gtgtagcagg taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt
    34501 ttttatttat agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta
    34561 ttgcgatcag cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct
    34621 atactgttgt tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc
    34681 tatggttaaa gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg
    34741 tttttgatac gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt
    34801 cgtttgattt gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg
    34861 tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt
    34921 gcaatgagtg tcagtatact cattttgaca agttgcagta caacctatac acaatcggct
    34981 tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat
    35041 ggtcaaaact tttcggaaaa agaagtgttc ttcaaaaaag gcgatgattt actgagtatt
    35101 ttgaaacgaa attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac
    35161 tcacaggatg agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact
    35221 actggtgcca aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa
    35281 ctataagttt cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt
    35341 tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt
    35401 gctgctctag gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt
    35461 ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt
    35521 ttgctgttat tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt
    35581 gaaggacgag caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt
    35641 ggggcacttg gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt
    35701 aatccgatca ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta
    35761 cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt
    35821 ttgcgcgaaa gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta
    35881 atcggtttgg tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca
    35941 tttgatgcga tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta
    36001 gcaaaagaat tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa
    36061 atcaagttag cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat
    36121 ttaagtccaa cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat
    36181 gaagcttact cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctga
//
LOCUS       DAYYFD010000020        35143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000020 DAYYFD010000000
VERSION     DAYYFD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.020"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACHA25_000900"
     CDS             <1..1152
                     /locus_tag="ACHA25_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM8762219.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHA25_000901"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACHA25_000901"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8762220.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACHA25_000902"
     CDS             2728..3003
                     /locus_tag="ACHA25_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8762221.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACHA25_000903"
     CDS             3009..3776
                     /locus_tag="ACHA25_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8762222.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACHA25_000904"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACHA25_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8762223.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACHA25_000905"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACHA25_000905"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8762224.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACHA25_000906"
     CDS             7920..8222
                     /locus_tag="ACHA25_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8762225.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACHA25_000907"
     CDS             8225..9388
                     /locus_tag="ACHA25_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8762226.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACHA25_000908"
     CDS             9414..12842
                     /locus_tag="ACHA25_000908"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8762227.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACHA25_000909"
     CDS             12863..13987
                     /locus_tag="ACHA25_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8762228.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACHA25_000910"
     CDS             13968..14414
                     /locus_tag="ACHA25_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8762229.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACHA25_000911"
     CDS             14398..14736
                     /locus_tag="ACHA25_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8762230.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHA25_000912"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACHA25_000912"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8762231.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACHA25_000913"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACHA25_000913"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8762232.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACHA25_000914"
     CDS             15893..16936
                     /locus_tag="ACHA25_000914"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8762233.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACHA25_000915"
     CDS             17095..17823
                     /locus_tag="ACHA25_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8762234.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACHA25_000916"
     CDS             17939..18184
                     /locus_tag="ACHA25_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8762235.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACHA25_000917"
     CDS             18181..18399
                     /locus_tag="ACHA25_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8762236.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACHA25_000918"
     CDS             18535..19074
                     /locus_tag="ACHA25_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8762237.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACHA25_000919"
     CDS             19067..21340
                     /locus_tag="ACHA25_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8762238.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACHA25_000920"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACHA25_000920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8762239.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHA25_000921"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACHA25_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8762240.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACHA25_000922"
     CDS             complement(23167..23436)
                     /locus_tag="ACHA25_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762241.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACHA25_000923"
     CDS             complement(23698..23826)
                     /locus_tag="ACHA25_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762242.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACHA25_000924"
     CDS             complement(23906..24361)
                     /locus_tag="ACHA25_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8762243.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACHA25_000925"
     CDS             complement(24499..26427)
                     /locus_tag="ACHA25_000925"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8762244.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACHA25_000926"
     CDS             complement(26624..27457)
                     /locus_tag="ACHA25_000926"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8762245.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACHA25_000927"
     CDS             complement(27649..28353)
                     /locus_tag="ACHA25_000927"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8762246.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACHA25_000928"
     CDS             complement(28376..29002)
                     /locus_tag="ACHA25_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8762247.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACHA25_000929"
     CDS             29328..31703
                     /locus_tag="ACHA25_000929"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8762248.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACHA25_000930"
     CDS             complement(31727..32218)
                     /locus_tag="ACHA25_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8762249.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACHA25_000931"
     CDS             32405..33325
                     /locus_tag="ACHA25_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8762250.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ACHA25_000932"
     CDS             33479..33823
                     /locus_tag="ACHA25_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8762251.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ACHA25_000933"
     CDS             complement(34013..34513)
                     /locus_tag="ACHA25_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8762252.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="ACHA25_000934"
     tRNA            35041..35124
                     /locus_tag="ACHA25_000934"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11642 a         6134 c         7244 g        10123 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattttcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaa
//
LOCUS       DAYYFD010000021        34414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000021 DAYYFD010000000
VERSION     DAYYFD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.021"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            342..1031
                     /locus_tag="ACHA25_000935"
     CDS             342..1031
                     /locus_tag="ACHA25_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8762253.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1018..2226
                     /locus_tag="ACHA25_000936"
     CDS             1018..2226
                     /locus_tag="ACHA25_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8762254.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2520..3791
                     /gene="serS"
                     /locus_tag="ACHA25_000937"
     CDS             2520..3791
                     /gene="serS"
                     /locus_tag="ACHA25_000937"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8762255.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="ACHA25_000938"
     CDS             complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="ACHA25_000938"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8762256.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6329..7024)
                     /locus_tag="ACHA25_000939"
     CDS             complement(6329..7024)
                     /locus_tag="ACHA25_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8762257.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="ACHA25_000940"
     CDS             complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="ACHA25_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8762258.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8223..8414)
                     /locus_tag="ACHA25_000941"
     CDS             complement(8223..8414)
                     /locus_tag="ACHA25_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8762259.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8435..9325)
                     /locus_tag="ACHA25_000942"
     CDS             complement(8435..9325)
                     /locus_tag="ACHA25_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8762260.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9312..10079)
                     /locus_tag="ACHA25_000943"
     CDS             complement(9312..10079)
                     /locus_tag="ACHA25_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8762261.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="ACHA25_000944"
     CDS             complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="ACHA25_000944"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8762262.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="ACHA25_000945"
     CDS             complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="ACHA25_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8762263.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="ACHA25_000946"
     CDS             complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="ACHA25_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8762264.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14650..15033
                     /locus_tag="ACHA25_000947"
     CDS             14650..15033
                     /locus_tag="ACHA25_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762265.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15201..15959)
                     /gene="jag"
                     /locus_tag="ACHA25_000948"
     CDS             complement(15201..15959)
                     /gene="jag"
                     /locus_tag="ACHA25_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8762266.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15977..16795)
                     /locus_tag="ACHA25_000949"
     CDS             complement(15977..16795)
                     /locus_tag="ACHA25_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8762267.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="ACHA25_000950"
     CDS             complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="ACHA25_000950"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8762268.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="ACHA25_000951"
     CDS             complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="ACHA25_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8762269.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17923..19257
                     /gene="dnaA"
                     /locus_tag="ACHA25_000952"
     CDS             17923..19257
                     /gene="dnaA"
                     /locus_tag="ACHA25_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8762270.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19455..20585
                     /gene="dnaN"
                     /locus_tag="ACHA25_000953"
     CDS             19455..20585
                     /gene="dnaN"
                     /locus_tag="ACHA25_000953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8762271.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20814..21056
                     /gene="yaaA"
                     /locus_tag="ACHA25_000954"
     CDS             20814..21056
                     /gene="yaaA"
                     /locus_tag="ACHA25_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8762272.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21043..22167
                     /gene="recF"
                     /locus_tag="ACHA25_000955"
     CDS             21043..22167
                     /gene="recF"
                     /locus_tag="ACHA25_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8762273.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22164..24110
                     /gene="gyrB"
                     /locus_tag="ACHA25_000956"
     CDS             22164..24110
                     /gene="gyrB"
                     /locus_tag="ACHA25_000956"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8762274.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24130..26601
                     /gene="gyrA"
                     /locus_tag="ACHA25_000957"
     CDS             24130..26601
                     /gene="gyrA"
                     /locus_tag="ACHA25_000957"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8762275.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26837..27136
                     /gene="rpsF"
                     /locus_tag="ACHA25_000958"
     CDS             26837..27136
                     /gene="rpsF"
                     /locus_tag="ACHA25_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8762276.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27186..27698
                     /gene="ssb"
                     /locus_tag="ACHA25_000959"
     CDS             27186..27698
                     /gene="ssb"
                     /locus_tag="ACHA25_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8762277.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27724..27960
                     /gene="rpsR"
                     /locus_tag="ACHA25_000960"
     CDS             27724..27960
                     /gene="rpsR"
                     /locus_tag="ACHA25_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8762278.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28103..30076
                     /locus_tag="ACHA25_000961"
     CDS             28103..30076
                     /locus_tag="ACHA25_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8762279.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30082..30534
                     /gene="rplI"
                     /locus_tag="ACHA25_000962"
     CDS             30082..30534
                     /gene="rplI"
                     /locus_tag="ACHA25_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8762280.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30808..32175
                     /gene="dnaB"
                     /locus_tag="ACHA25_000963"
     CDS             30808..32175
                     /gene="dnaB"
                     /locus_tag="ACHA25_000963"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8762281.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEVNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32955..34247
                     /locus_tag="ACHA25_000964"
     CDS             32955..34247
                     /locus_tag="ACHA25_000964"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8762282.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10503 a         6691 c         6816 g        10404 t
ORIGIN      
        1 gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa caaataataa
       61 tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac atttctagct
      121 tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc cgagagacgt
      181 aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac caactagatg
      241 tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct gctttttttt
      301 gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt ttgattgctg
      361 acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag gggtatgaag
      421 cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc cctgatattg
      481 aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta gtgaaagagc
      541 tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact gatatggata
      601 agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt aatccgttag
      661 aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt gccccagatc
      721 agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat gaagttaaaa
      781 caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc tacttgcttg
      841 ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag
      901 aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga gataagattg
      961 aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc tataaaattg
     1021 acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa
     1081 atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct attaaatgtt
     1141 tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac gaatgcgatt
     1201 tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg gaacggccgg
     1261 aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg gcgcttgatt
     1321 cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta catcgcaaac
     1381 ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg agtggtaaca
     1441 agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg taaaatcgaa
     1501 aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc actcacgtcc
     1561 atcattggat acctaggact gatagaagat ggccagtatc actcagaaga agatctttta
     1621 aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt ggacgactta
     1681 tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc ctttgacatg
     1741 atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa aaaaaatatc
     1801 caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac cgaaaaactt
     1861 gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg agcaacgaag
     1921 atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa aaataacgga
     1981 gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg tgtagaagaa
     2041 tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca aagcattgtg
     2101 gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc tttcatcatg
     2161 caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt tattgacgaa
     2221 agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac acagaaccca
     2281 gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa ggataatttg
     2341 gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca acagccgtta
     2401 tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt cagactgatt
     2461 cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga ggaaatcata
     2521 tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc
     2581 ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat cgtcgtaagc
     2641 tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc
     2701 aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg
     2761 gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg aaacaaattg
     2821 caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat gaagaggaca
     2881 atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca aaaccacact
     2941 gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa gtagccggta
     3001 gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc tacaacttca
     3061 tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct tatatcgtca
     3121 acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat gttttccaat
     3181 tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact aactattata
     3241 atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta agccctgctt
     3301 ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt cttcaccaat
     3361 tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa gaattggaaa
     3421 aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat cgtgtgatgg
     3481 ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta gaagtatgga
     3541 tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa gatttccaag
     3601 cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat actcatactc
     3661 tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa aactaccaaa
     3721 atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg aatctgaaag
     3781 ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga aaaattttgt
     3841 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     3901 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
     3961 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
     4021 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
     4081 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
     4141 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
     4201 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
     4261 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
     4321 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
     4381 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
     4441 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
     4501 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
     4561 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
     4621 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
     4681 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
     4741 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
     4801 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
     4861 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     4921 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     4981 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     5041 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     5101 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     5161 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     5221 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     5281 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     5341 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     5401 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     5461 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     5521 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     5581 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     5641 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     5701 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     5761 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     5821 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     5881 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     5941 ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     6001 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     6061 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta
     6121 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     6181 aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt
     6241 cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc cctagatatc
     6301 tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa cttccaataa
     6361 tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg ataatgataa
     6421 ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca
     6481 gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc attcctggtt
     6541 tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac atcatataaa
     6601 agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa gctggcgttg
     6661 ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca agcaacagca
     6721 acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct gctggcttat
     6781 tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct gttcttccgc
     6841 ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact ttttcttctt
     6901 cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt tgatttcttt
     6961 tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat tgttctggtt
     7021 ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac aaaagacatt
     7081 ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga ataacattac
     7141 gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag cttcttttgc
     7201 tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg cacggataaa
     7261 tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga ttcctttttt
     7321 gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta agcctaacaa
     7381 atcataagca gcacggatca actgatccaa acctgattct tcgattccta aagcttctaa
     7441 aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac gagcactgac
     7501 cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta cataatgatt
     7561 cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa ttggttttgt
     7621 cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt cgatcgttcg
     7681 ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta gttctgccac
     7741 tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt tgttgataga
     7801 ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta gtggatcaac
     7861 acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt gacaaatagc
     7921 gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt tacttgcgcc
     7981 ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg
     8041 tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc ctacgttggg
     8101 gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga tcgcattaaa
     8161 caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg ccataaattt
     8221 attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc aaaatctcga
     8281 cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc tgcgcccatt
     8341 cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg
     8401 tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat tgaatattca
     8461 agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg atcttacctt
     8521 tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa cgatcttcgc
     8581 tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc ttttcttttc
     8641 catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt aaattttcag
     8701 ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct aacagcgtac
     8761 gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga tccggtaaag
     8821 aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga ctagctactt
     8881 ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct tcttctaacg
     8941 gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc gcttcttcgt
     9001 caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac gctcgaaaac
     9061 gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg atgatgggtt
     9121 gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact tcttcaaatg
     9181 ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg acaacggtat
     9241 catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca tcgattcctc
     9301 ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc ttggtagact
     9361 tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact aggtgcttct
     9421 gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt
     9481 acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt cagtaagact
     9541 ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt attcagtaat
     9601 tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat cgaatcactt
     9661 gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat caaaatataa
     9721 tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct tgccatcatt
     9781 gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat actcaaattt
     9841 tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag aacatcatag
     9901 atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt tccttgtgca
     9961 tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc tagattcacc
    10021 gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat ttgtgccatc
    10081 tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt gggtatttct
    10141 ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag acattcggca
    10201 aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct tgttttgctt
    10261 ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct tcttgtttag
    10321 ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga gcagtgacaa
    10381 ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg tcatgatata
    10441 aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt cgtttattca
    10501 gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa
    10561 accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct acttcaaatg
    10621 ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc gtaatagctg
    10681 taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg aattgattca
    10741 tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat tcttctggta
    10801 tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct ccctacttta
    10861 acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt gaaaagtaag
    10921 aaaagccgat agactttttc tttctagtat gactcataaa atatacacag aagagagaac
    10981 tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt acgtattacg
    11041 ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg atacttaggt
    11101 ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt tggattttct
    11161 gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg atgttttctg
    11221 gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct tttttgatgt
    11281 agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc aattcttcat
    11341 tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt aaaaagtcgc
    11401 ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag tcttggactt
    11461 cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca actgcttctt
    11521 ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg tgacccattt
    11581 ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca gcacgtgaag
    11641 tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc atgacaccga
    11701 tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc aaagctgcat
    11761 tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta ccatttgttt
    11821 gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg agctggtgtg
    11881 gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact ttttctaatc
    11941 cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta gataaacctt
    12001 gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga tgacgtggtt
    12061 tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg cctacccctt
    12121 caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg
    12181 tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca ctatctggtg
    12241 tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta acagaatagt
    12301 cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc
    12361 caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc ggacctgatg
    12421 aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct gtaataacaa
    12481 ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa acaccatctt
    12541 cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct ttttcgatcg
    12601 tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat gctcggacag
    12661 ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg atattcttac
    12721 ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct ggtccgccta
    12781 cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt aataatgttt
    12841 ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa
    12901 cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc cttttatttt
    12961 cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact gtcgccgaca
    13021 acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc cacaggcatt
    13081 cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc taacaacgcg
    13141 atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc gctgaagaat
    13201 aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac ggaaattgaa
    13261 aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg tgctggcaaa
    13321 tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa taattgttca
    13381 tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc tgcttctgct
    13441 aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt ttcccgaatt
    13501 ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc ttcgattaca
    13561 tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa caaatggttt
    13621 aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa cccttcccgc
    13681 agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg gatcatctgc
    13741 gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc tggataatca
    13801 atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga gcggatcaaa
    13861 gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc tgtttttgcc
    13921 cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct
    13981 cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa
    14041 atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc ttcccgcgta
    14101 aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg attgctcttg
    14161 ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa tcgtttgttt
    14221 ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa acgaacgata
    14281 ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta
    14341 atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg cccactccac
    14401 cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg attaaattct
    14461 ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt agatgaagtt
    14521 gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc tttgcgttct
    14581 ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt aaaaaaaacg
    14641 gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa atatgtgagc
    14701 ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta
    14761 tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc
    14821 ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt aacaacgaag
    14881 aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt
    14941 gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc ttatggaaca
    15001 gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa agcggatcaa
    15061 aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact tcatcgtata
    15121 ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg tcaacgtgat
    15181 ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac taaataacga
    15241 taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa tgcagcatgg
    15301 atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc tgtccgtttt
    15361 actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt ttcacggtaa
    15421 ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat gaagacttgt
    15481 gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat gccttgcttt
    15541 tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt gacaagagct
    15601 ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt taaagctgct
    15661 tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc
    15721 tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc tactgtttca
    15781 gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg acccattcct
    15841 aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt ggccaaaccc
    15901 aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata aatcggcatt
    15961 agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc ttaagagcac
    16021 gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt
    16081 tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag tataatgaaa
    16141 gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg aaattcatga
    16201 tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta cttgcaaaag
    16261 taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct aagttcaacc
    16321 ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat aacgccatca
    16381 agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg ccattttctg
    16441 catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct cttgaactat
    16501 attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc atgctctttg
    16561 tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat aaaatgatcc
    16621 ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa taaacaatat
    16681 aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa ccggataaga
    16741 cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc ttcacttact
    16801 cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat tggagcgaac
    16861 ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat ccaactccgg
    16921 atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga cttcattgcg
    16981 gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat gaggctgtcc
    17041 cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc ccgtatggaa
    17101 cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta gttttctttc
    17161 cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc ctttcatcat
    17221 ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac
    17281 ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg
    17341 tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat
    17401 atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat
    17461 aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca
    17521 caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
    17581 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
    17641 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
    17701 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
    17761 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
    17821 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
    17881 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
    17941 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
    18001 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
    18061 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
    18121 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
    18181 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
    18241 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
    18301 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
    18361 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
    18421 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
    18481 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
    18541 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
    18601 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
    18661 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
    18721 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
    18781 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
    18841 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
    18901 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
    18961 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
    19021 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
    19081 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
    19141 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
    19201 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
    19261 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
    19321 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
    19381 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
    19441 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
    19501 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
    19561 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
    19621 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
    19681 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
    19741 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
    19801 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
    19861 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
    19921 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
    19981 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
    20041 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
    20101 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
    20161 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
    20221 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
    20281 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
    20341 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
    20401 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
    20461 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
    20521 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
    20581 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
    20641 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
    20701 ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg ttttattgcg
    20761 aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg gcattgaaac
    20821 aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag
    20881 ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg
    20941 gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag
    21001 aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg
    21061 tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc
    21121 atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta
    21181 gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat
    21241 caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta
    21301 ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact
    21361 tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc
    21421 agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat
    21481 ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa
    21541 ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca
    21601 gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg
    21661 gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc
    21721 accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca
    21781 cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat
    21841 cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga
    21901 cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag
    21961 acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt
    22021 caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca
    22081 cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg
    22141 aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag
    22201 agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg
    22261 gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg
    22321 ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa
    22381 aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa
    22441 aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg
    22501 gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgtaaacg
    22561 cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat
    22621 atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa
    22681 caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt
    22741 caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga
    22801 ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatca
    22861 aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt
    22921 tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc
    22981 attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga acacatgaat
    23041 caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga
    23101 tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta
    23161 tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt
    23221 ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg
    23281 aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc agagctcgtc
    23341 tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc
    23401 tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg
    23461 tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca
    23521 tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg
    23581 caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg
    23641 atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg
    23701 cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag
    23761 ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta
    23821 cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc
    23881 ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc
    23941 aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg
    24001 atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc
    24061 gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga
    24121 ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa
    24181 atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat
    24241 gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt
    24301 gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa
    24361 taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc acagccattc
    24421 agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt
    24481 gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt
    24541 gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca
    24601 gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta
    24661 ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg
    24721 ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat
    24781 tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta tgaaacaggt
    24841 aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa
    24901 cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt
    24961 tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc
    25021 cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc cgttatcttg
    25081 aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc
    25141 gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta tatcgaacat
    25201 caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga agcaagagca
    25261 cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc
    25321 cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt cgatttatcc
    25381 gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg
    25441 gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt
    25501 ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa
    25561 cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat
    25621 gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa
    25681 cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg
    25741 ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg
    25801 ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac
    25861 gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc gagtgaaagc
    25921 atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg
    25981 acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat
    26041 ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat
    26101 ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa
    26161 gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct gcgtgaggaa
    26221 gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt gatcacagaa
    26281 aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa
    26341 gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt
    26401 tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta
    26461 tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct
    26521 gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa
    26581 gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa gaataaccga
    26641 ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt
    26701 cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta gagcttgtga
    26761 gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac
    26821 agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa
    26881 cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa
    26941 cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa
    27001 cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc
    27061 tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt
    27121 taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga
    27181 ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt
    27241 acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa
    27301 accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag
    27361 aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa
    27421 ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca
    27481 acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc
    27541 aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc
    27601 aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt
    27661 caacgatcga catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat
    27721 agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct
    27781 aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa
    27841 cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt
    27901 actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa
    27961 taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg
    28021 taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt
    28081 agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct
    28141 cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt
    28201 tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat
    28261 gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga
    28321 aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc
    28381 aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt
    28441 aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt
    28501 ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt
    28561 taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat
    28621 tggtatttta tctattgata actatgcaga tgccattgat aaattagatg acaaagagat
    28681 ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt
    28741 ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa
    28801 aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca
    28861 gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat
    28921 cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt
    28981 cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa
    29041 tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga
    29101 tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc
    29161 tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa
    29221 tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga
    29281 attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct
    29341 cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca
    29401 atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc
    29461 tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat
    29521 ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc
    29581 agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt
    29641 tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc
    29701 ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc
    29761 tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa
    29821 aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg
    29881 gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat
    29941 catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac
    30001 gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat
    30061 atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt
    30121 aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat
    30181 aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca
    30241 caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa
    30301 aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt
    30361 tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat
    30421 aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt
    30481 aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc
    30541 ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta
    30601 tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc
    30661 ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact
    30721 atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga
    30781 agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg
    30841 caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc
    30901 attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt
    30961 tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa
    31021 gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac
    31081 ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa
    31141 aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag
    31201 caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg
    31261 gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa
    31321 aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa
    31381 ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt
    31441 ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca
    31501 gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg
    31561 ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa
    31621 gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat
    31681 gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa
    31741 aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagt taacggtaaa
    31801 gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag
    31861 ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac
    31921 aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc
    31981 gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga
    32041 aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca
    32101 cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca
    32161 agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga
    32221 agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg
    32281 gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg
    32341 acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct
    32401 ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg
    32461 ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg
    32521 ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa
    32581 agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt
    32641 ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc
    32701 aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg
    32761 tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt tttaataacc
    32821 attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat aaaaatcatt
    32881 cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta aataatatag
    32941 aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc gatgaaggga
    33001 aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg
    33061 gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg catttgattc
    33121 cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc
    33181 ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt
    33241 tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg gaccaattac
    33301 aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc ggacctgcct
    33361 atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat aaagaaattt
    33421 ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta aaaatgtttg
    33481 attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac ggacaacaaa
    33541 tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat gcaggcaaac
    33601 gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga acttatcctt
    33661 ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt gtagggcctt
    33721 caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac
    33781 catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta ggacgagaat
    33841 acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc
    33901 attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat gtattaagtg
    33961 gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta atctaccact
    34021 atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct
    34081 ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac gcacgtaact
    34141 atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct gtcggacctg
    34201 atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga taaaaacgac
    34261 gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac gataataata
    34321 acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt ctcttgaaca
    34381 ctgtttattc agaataaggg actgtgacaa aaac
//
LOCUS       DAYYFD010000022        33499 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000022 DAYYFD010000000
VERSION     DAYYFD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.022"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..415
                     /locus_tag="ACHA25_000965"
     CDS             59..415
                     /locus_tag="ACHA25_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8762283.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="ACHA25_000966"
     CDS             405..671
                     /locus_tag="ACHA25_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8762284.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="ACHA25_000967"
     CDS             830..1831
                     /locus_tag="ACHA25_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8762285.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="ACHA25_000968"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="ACHA25_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="ACHA25_000969"
     CDS             3055..3300
                     /locus_tag="ACHA25_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762286.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="ACHA25_000970"
     CDS             3398..3619
                     /locus_tag="ACHA25_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762287.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="ACHA25_000971"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="ACHA25_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4761..5300
                     /locus_tag="ACHA25_000972"
     CDS             4761..5300
                     /locus_tag="ACHA25_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762288.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            6320..7489
                     /locus_tag="ACHA25_000973"
     CDS             6320..7489
                     /locus_tag="ACHA25_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8762289.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            7962..8516
                     /locus_tag="ACHA25_000974"
     CDS             7962..8516
                     /locus_tag="ACHA25_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8762290.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(8689..9126)
                     /locus_tag="ACHA25_000975"
     CDS             complement(8689..9126)
                     /locus_tag="ACHA25_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8762291.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            9174..10121
                     /locus_tag="ACHA25_000976"
                     /pseudo
     CDS             9174..10121
                     /locus_tag="ACHA25_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            10392..10827
                     /locus_tag="ACHA25_000977"
                     /pseudo
     CDS             10392..10827
                     /locus_tag="ACHA25_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            10843..11676
                     /locus_tag="ACHA25_000978"
     CDS             10843..11676
                     /locus_tag="ACHA25_000978"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8762292.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            11921..12946
                     /locus_tag="ACHA25_000979"
     CDS             11921..12946
                     /locus_tag="ACHA25_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8762293.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            13084..13209
                     /locus_tag="ACHA25_000980"
     CDS             13084..13209
                     /locus_tag="ACHA25_000980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762294.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            13234..14349
                     /locus_tag="ACHA25_000981"
                     /pseudo
     CDS             13234..14349
                     /locus_tag="ACHA25_000981"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            14432..15328
                     /locus_tag="ACHA25_000982"
                     /pseudo
     CDS             14432..15328
                     /locus_tag="ACHA25_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            15344..16168
                     /locus_tag="ACHA25_000983"
     CDS             15344..16168
                     /locus_tag="ACHA25_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762295.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            16200..17510
                     /locus_tag="ACHA25_000984"
     CDS             16200..17510
                     /locus_tag="ACHA25_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8762296.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            17658..18917
                     /locus_tag="ACHA25_000985"
     CDS             17658..18917
                     /locus_tag="ACHA25_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8762297.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            18927..19799
                     /locus_tag="ACHA25_000986"
     CDS             18927..19799
                     /locus_tag="ACHA25_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762298.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            19811..20641
                     /locus_tag="ACHA25_000987"
     CDS             19811..20641
                     /locus_tag="ACHA25_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762299.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            20681..22864
                     /locus_tag="ACHA25_000988"
     CDS             20681..22864
                     /locus_tag="ACHA25_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8762300.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            22925..24145
                     /locus_tag="ACHA25_000989"
     CDS             22925..24145
                     /locus_tag="ACHA25_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8762301.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            24142..25602
                     /gene="gtfA"
                     /locus_tag="ACHA25_000990"
     CDS             24142..25602
                     /gene="gtfA"
                     /locus_tag="ACHA25_000990"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8762302.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            25679..26785
                     /locus_tag="ACHA25_000991"
                     /pseudo
     CDS             25679..26785
                     /locus_tag="ACHA25_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            27107..27316
                     /locus_tag="ACHA25_000992"
     CDS             27107..27316
                     /locus_tag="ACHA25_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8762303.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            27309..27458
                     /locus_tag="ACHA25_000993"
     CDS             27309..27458
                     /locus_tag="ACHA25_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8762304.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            27612..28277
                     /locus_tag="ACHA25_000994"
     CDS             27612..28277
                     /locus_tag="ACHA25_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8762305.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(28561..29646)
                     /gene="xerS"
                     /locus_tag="ACHA25_000995"
     CDS             complement(28561..29646)
                     /gene="xerS"
                     /locus_tag="ACHA25_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8762306.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            29980..30213
                     /locus_tag="ACHA25_000996"
     CDS             29980..30213
                     /locus_tag="ACHA25_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8762307.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            30210..30617
                     /locus_tag="ACHA25_000997"
     CDS             30210..30617
                     /locus_tag="ACHA25_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8762308.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(31754..32272)
                     /locus_tag="ACHA25_000998"
     CDS             complement(31754..32272)
                     /locus_tag="ACHA25_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8762309.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            32639..32887
                     /locus_tag="ACHA25_000999"
     CDS             32639..32887
                     /locus_tag="ACHA25_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762310.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            33390..>33499
                     /locus_tag="ACHA25_001000"
     CDS             33390..>33499
                     /locus_tag="ACHA25_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8762311.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT        11630 a         4996 c         6145 g        10728 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt ttgatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt tttcagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac
     6001 tatattttag gtttcatatt atataaatta ataatatact accatttgtt ggtagtataa
     6061 tttaaaggta ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt
     6121 aataagagtt tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag
     6181 tgatataacg aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga
     6241 tatttagggt agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat
     6301 tttataggag gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa
     6361 taaaggtgat ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt
     6421 gcaggctgaa ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc
     6481 aggtaattcc cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa
     6541 tttggtaatt cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa
     6601 aagaaccaat gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat
     6661 gtctgaaatc tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat
     6721 ttcaaatatg actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga
     6781 atccagatac tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt
     6841 ttcaaaagaa gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct
     6901 tgggtttagt cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct
     6961 aaaagaccgt ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa
     7021 cgatagtatt catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc
     7081 tagaaatatc gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa
     7141 attagtttat caagcaagtt caaaagaaga agcaaataat caaataaggt ttatgataga
     7201 taaatggaaa aagcagtatc cacgagtggt taaattgctc atgaatcctg ctatattaac
     7261 cttttataat ttccctccgt caatcagaag aactatttat tcaactaatt tgattgaagg
     7321 ttttaacaaa caattaaaga aatataccaa aagaaaagag caatttccta atgaagaatc
     7381 tctggagaga tttctagtat ctcaattcaa caactataat caaaagtttc tatgccgagt
     7441 tcataaaggt tttaaagaaa tacatgatac attagaatca atgttttaac ttgatcaaca
     7501 gaatgggctt ttccatttac acaaaattat tgacgctccc gacaaaatta tgatcttgcg
     7561 taaagttata aaatattaat aaaaatattt tattaatgaa aagcttggta gcacggttat
     7621 tcatatgcta atttatttca ctttatagta aggggagttc ttagtatatt gttcaaactt
     7681 aaatttcata taatttaatt atgtaaatta gctattctaa gaacttctac ggataactgc
     7741 ccgtctaaaa tttaaataag agcttagaat cttcatataa gtaaggttaa tttttttgta
     7801 caaaaaagtc ggaatattct gagttactga aatgatataa aatcataaac taaatccctt
     7861 gattttattg actttcaaaa gtttatatat ataagttaat gattatggta aaatatatac
     7921 ataatcatta actcagaata tttttaagaa gggaaacaat agtgagctat aatgtacaac
     7981 cgttaaggac aaatgaagag atacaagatt ttcagttttg gctaagaaga acaagtaatc
     8041 cggaaagaga tagtttttta tttttgtttg gaatcaataa tgggttacgt atgtcagata
     8101 ttataggatt aaaagttgga gatatacgag gcaaaagtaa gcctattatt gttgaacata
     8161 aaacaggtaa gcgaaaaccg atttttatag ataatttgag agaagaaatt ttattatata
     8221 ctgaaggtaa agaagaaaat gattggttat ttccgagtcg gcaacaaggt cgacatataa
     8281 caagggatcg tgtctatcag atatatgcag atatagcaga aaaattagga cgagatgata
     8341 taggaacaca tacattaaga aaaacttttg ggtatcacta ctacaagaaa actagagaca
     8401 ttgctacctt aatgtttatt tttaatcata gctcgcaagc aattacaaaa aggtacattg
     8461 gtataactga agatgaaata ggagcatcac ttcgtggatt taaactaggt gtataattta
     8521 aaaattttta taaacagtaa agacaaagat taaaagctat ttttatctag aacgatttta
     8581 tttcataaag ttcgcggaat aaaagctggg gttactggta cttggtatag gttcgtgtta
     8641 tataaactaa gtaattaaat aacattttta ggttggaaaa ggatgaaatt aaaaaaagag
     8701 aatcaatacg gtctcaaaaa cgcaaacgct gcggaataag aaaaatactg ataactcaac
     8761 gtttagcaga ctatctaata gggatagtct gcttttttcg tttttgacca tacttttgat
     8821 cttctgcaaa cttccttgct tttcctctgt agaactcatg aaatctgcaa aacgaattgc
     8881 cgtttcctct gtctgctttt tcgcgatatg ggtgtatata tcatccatag tggttttaat
     8941 atctgtatgt ccaagtcgaa catacacttc tttgacagac gcaccagatt caaagagcag
     9001 actacaatgg gtatgacgaa aaccatgaag tgtgatatgt tttaagttat tctctttaat
     9061 gatgattttc aaattgtgat ttacatagtc taaataaagg ggcttatttt cttctgatga
     9121 aaacaaaagt tgttcaccat cttttatagg tttgattctc atttttagat attatgcccg
     9181 cacgtattca tcacatttct attgtgaatc gctttattcg accgacattt gacttttatc
     9241 ataatatact cggtttaaaa ttactaatga aaacaattaa tcaagacgat cataccatgt
     9301 atcacctgtt tttttcagat aatcaccacc gtgtgggaac tgaactgact ttttttgaag
     9361 tacaagaagg gaacaaccag ttttttggaa ccaatacgat tgaacgaacg atactaaaag
     9421 ttccttccga agcatcattg catttttggg aaatatattt tgaaacacaa ggcgtttgcc
     9481 attatggaat cgaatcattt tctggacgac ctatcttacg ttttgaaggg ccagatgcaa
     9541 cacgattagc tttagttccc ttacgcgaat ttgaagatat tgatgattat ttcccgtatg
     9601 tcacagacca gatccctaca gagcatgcca ttttaggaat tgatgccatt caactacgcg
     9661 tacaatattc cgatgcgact gaacgagagt tattgtcggt aggctggtag cacaaagaat
     9721 acgtcagttt cttttcgggt catcaacgag tgacaatctt agaaaatcaa gacactcagt
     9781 tctatcaaga agttcagatt attcaagatc gtgagaatcc agttgccatt gaagggattg
     9841 gtagcgtcca tcatgttgcg tttggcgtag aaaataaatc tgatttacaa cgcatcgaca
     9901 aacaattaca agagcgcaat tttataaatt caggaataaa agatcgtgaa ttttttgtat
     9961 cactttatta tcgtgaccca aatcaattat tgattgaaat tgctacaggg gaaggaaacc
    10021 tagatgcaaa agcgtatgaa aatcaaagtc cacgctttga agaaattcct ttatttttac
    10081 cgcagtactt aaatttcatt cgtgaacaag tggagcaata attagctaat caaatccatg
    10141 acgaagagta agaggttgac tcataagtca aaaaataaag aaaaacgcat caaatccatt
    10201 ctaaagaatg ttgatttgat gcggttccta tttttataaa ctatcatttt cgactaataa
    10261 aactacttat gagtcagcct ctcttttttt cttgattttt atacctcttt ctaggtaaac
    10321 taaacatctg ccaaaaatac cgtgtctcat tgaaacattc tcaactattt caaaataaag
    10381 gggagtacca aatgaacgag tatattgaaa ttatccacgc aagagaaaca acttaaaaat
    10441 gtcagtctca aaattcccaa aaataaaatt agtattttta ccagcgtatc tggttcagga
    10501 aaatcatcga ttgtttttga tactattacc caagaagctg gtcgtcaact gaatgacacc
    10561 tatgatagtt ttacccgttt atttttacct aaatacagtc gtccagaggt tgatgagatt
    10621 gataatcttt ctacggcgat tgtggtcaat caaaagaaga ttggtggcag tgcgcgctca
    10681 acgttaggga cgattactaa catcaatgcg ttatttcgtg tgttgttttc atgttttgca
    10741 acacctagtt taggatatgc aaatgcttat tcatttaatg atcctagtgg aatgtgttct
    10801 gtctgtcagg gaatcggtaa gacgattgca tttttaatag gattgcttta tttttcggct
    10861 attgataatg ctgattttgt attttctgat ccatttaccg cagaagtttt gtctggaatt
    10921 gaggaacagg caaagaatca tggttatttt gtattggtgc ataatgttaa ttcagcagaa
    10981 gatgttaaaa ctattcagag aaattggcgt tttgaggggt tcattgttgt tggtgtacgt
    11041 gtaaaagaat ttttggaatt aaataacgtt tttgatggtc ctgttgttta tattgatacg
    11101 catctttcaa aagagacatt aagagaactt gaacaacaaa tgaatcgttt atttgtaaat
    11161 acaaatgatt accaagctag tacattggca gtggaacatt tgattcatta tggtcatgag
    11221 aaaatagcgt ttctttcttt tgactacatt gtaggtgaac caagtgttat tcaagaacga
    11281 catcaagctt atatcgatac attaaagaaa tatcatttaa cctttaaatc gcattggata
    11341 tataacaact ctgaatttga gcgaatttat caagaattag ctgaatttac tgcaattgtt
    11401 gttactggag attatttagc ggctaaatta gtaaaatttt taaaaaataa gaataaacga
    11461 gtgatggaac aattatcgat tattggattt gatgatatta cttttgcaga attaatggat
    11521 cctgcgttga caacaatacg actaaatcca ggtaacaagg gggcgattgc ttttcaccaa
    11581 ctgattggac tggccaaaca agaagaaata aaaaatcaag tactactatt agagggagaa
    11641 ttgatttctc gagactctgt tcacaaaatc aaataaaaaa gctctgtgaa ataagtatag
    11701 ttaacaataa aatatccgaa cgatagagag aatgaactgt gatctagcag ttcctcgtcg
    11761 caaattcact agaattagca ttatttactc taaattgttc ggatattttt tcttgttttt
    11821 aattatgatt acagtgccta tttttaacga aatttgctta ttttcaaaat gtatgattat
    11881 atgttagtaa acactttcaa atatgaaaag aggagctatt atggtaggaa ttagggatgt
    11941 tgcaaaacgg gcgggtgtgt ccattgcaac agtatcgcgc gtgctaaatg aggatccgac
    12001 actttcagta accgatgaaa ccaaacgaaa gattgcagaa tcggttaatt tttatcatta
    12061 tgtcaaaaaa agtcccgttg caaaaaattt aacaaatatt gcattaataa cgacagtttc
    12121 tgaagtagat gagttggaag atccctattt tcgaacgatt cgcagaggga ttcaagtcga
    12181 agcagaacaa agcaaagtca atttgaagaa aattattcgt ttgtcagaaa caccgttgga
    12241 gcaagacgat ttgctaaaat gtcaaggtgt gttgattatt gggcaggtgc tatcttctgt
    12301 gattgaagaa gtatcagcaa ttaattcgaa tgtcgtagtg attgatgacc ctagtattga
    12361 agctgaagta gatgcagttt atactgattt aacaaaagca acgaatcagc atttagatcg
    12421 attgtataaa aaaggacacc gaaacattgc gtttatcgga ggaggacgtg ttcgtttaga
    12481 tagccaagga aataaacacg tgtcagatga tgattatcga aaaatagcct atgagacgtg
    12541 gatgaaacaa aaaggattag aaaaatatag tcaaatttat ttagaaggct ggaccacact
    12601 tgacggaatg caggcagcag atttactttt gaaaaaacat gctaaagata agttgccgac
    12661 tgctatcatg gtaggaaatg atcctattgc tgtgggggtt tatcgttcac ttcaaaaaaa
    12721 tggtctacat attccagaag atatttcgat tgtgagtttt gataatattg aagttgctga
    12781 atttttaaca cctgcattaa atacggtgaa tgtgaataca gaagaaattg gaaaactagc
    12841 agttcgcatg gttaatgaac gaataaaaga agttagaaat gtttcgatca aagtgattgt
    12901 ttctaataat attattgtta gagaaagtga aaaaaatatc gattaaaaaa gtaaacatcc
    12961 aaaaaagggt gtttactttt ttgtttacta atatttttag taaaaaatct cgacaaaaca
    13021 ttaagtaaac agttatggtg ttgttaacaa aaagaaaacg cattcaaaac aggaggaatt
    13081 aagatgaaac atttcaaaaa aatagcttta acagggatag ctgttttaac tgggacatta
    13141 ctcgcagcat gtggtggggg agatagtaat tctaaagacg ggcaagtaac tttaagctat
    13201 gcgagttgag atactgacca agctccagga ctaagaaaaa tgttagatga atttgaaaaa
    13261 caaaatcctg atatcaaagt agaaatggag acaacgcctt gggatcaata ttgggttaaa
    13321 ttagaagctg caacaacggg aggtaatatg ccagatgtag taaccatgca ctctagcgaa
    13381 tcctataaat atatgtcaca gaatgctttg atgggacttg atgatgttat caaagaaaat
    13441 gatattgaca tgggaaactt tactgaagga attgctgatt tctatacgct tgaagacgaa
    13501 ctatacgcaa tccctaaaga tgcttctgta gtggggctat ggtacaacaa agaattattt
    13561 gataatgcag gtgtcgatta tccagatgaa acatggacat gggaaacact cagacaagct
    13621 gcaattgatt taacagatga atcaaaaggc atctatggtt ttgcatcaca tattggaaca
    13681 gagactggct actggccatt tatttatcaa aatgggggag aagtatttgc cgatgacaat
    13741 acaaaatcag gtttgaatac cccagaagca caagaagctt tagcttttta tcgagatatt
    13801 gcacttgttg atcatgcttc accaacaatt acagaattac aagatcctga taagttaagt
    13861 cgttttcaat caggtagatt agcaatgatg attgaaggaa actggtttgc accgggattt
    13921 ttgaaaaatg actatctagt tgaaaatgtt gcttctgccg tgttacctaa gggaaaagtt
    13981 aatgcaacaa ttacaaatgg acttggttgg gctgccagtg cgagtacaaa atacccagaa
    14041 gaagtgaaaa aactaatggc atttttagct agtgaggacg ccaataaaat ccaagcggaa
    14101 acgggggcat caatcccagc actaaaaggc tttgaagacg agtgggctgc ttcttcagat
    14161 acaattgatt tgagtgcttt tggaaaaatg ttggagtatg gagtccctcg tccatacaat
    14221 aaagatggat tacgagctga acaagcagaa gttcaaatta ttaaccgtat tctgtctggt
    14281 gaagtaactg ttgaagatgg aaccgctcaa attgaaaaag aagtcaatgc gatattgagc
    14341 ggaaattaaa ttagttaatt atacaatata caggcgaaag gattctattt cgtctgtata
    14401 ttaatggatg aataccaaaa agggggatat tatgaaaaat cgaatttttg gaaaatatcg
    14461 taaagatcaa gtgatatggg gctatatttt tatttttcca acggcattag gattaatttt
    14521 attaaatata tggccagcaa ttcaaacagc aattttaagt tttcaaaaaa cggtagggtt
    14581 cggtgatact gaatgggtcg gattaagaaa ttaccaaaaa atgtttgcag acggtgaagt
    14641 ttttcagtct ttattaaaca cattaattta tgcagtggtt tcgataccta gtattgttgt
    14701 attatccttg ttggtggcag ttttaatgaa tagaaaagtt aaaggattgt cgatttatcg
    14761 gacaatttat tttctaccag tcgttgctgc accatccgca gtcgcaatga tttggcgctg
    14821 gatgttcaat aatgattatg gactgattaa taacatgttg gctaaaatag gtctcgatgg
    14881 accagcatgg ttaacggatc catcgattgc gatttattct attatcattg taggagtttg
    14941 gagtgctatt ggttataaca tggtgttatt actagccggc ttataagaaa ttcctaaaga
    15001 ttattatgaa gcagcggaaa ttgatggtgc tagtccaatc cagcagtttt tcaacattac
    15061 gttaccatta gttacaccaa cattattttt tgtagttgta acaacagtta tcaatgcttt
    15121 ccaagtattt gatgttattt ttatgatgat tactcctaat agtaccgcca tgtttaaatc
    15181 acaatcatta gcctatttat tctataaaca ttcatttatt ttaaatgaca agggatatgg
    15241 ttcggcaatc gttatgttcc ttttagtgat tatattaatt attactgcaa ttcaaatgaa
    15301 gttgcagaaa aagtgggtaa actattaagg aggctatcaa attatgaaaa ataaattttt
    15361 ttcaaatgcg ccaatccata tcatgcttat tttaggtggt attgcaatgg cattaccttt
    15421 tatttggatg attttaactt cagggaaaac gttggttgaa tctacccaaa ttccacctac
    15481 aattttccct gagaaatttc agctaagtaa ctatcaacaa gtacttgatt tattaccatt
    15541 catggctttt tatatcaata catttttaat ggttgttgga cgagtgattg gctcggtttt
    15601 attttcagca atggctgcat atgcttgtgc gcgtctaagt tttccaggga aaaatatcct
    15661 tttcagctta gtacttattc aaatgatgat tcccggttcc atttttatta ctccacagta
    15721 tttattagca caaaaattgg gtattttaaa tacaattcca gcattaatat taccaggggt
    15781 agttagtgcg tttggtacgt ttttattaag acaatttttc ttaggtttac ctgatgaatt
    15841 ggaggaagcg gcctatttag aaggtgccaa tatttggacg atctttttca aaattatgtt
    15901 gccgcttgct aaatcaggat tggttgcact tggaatattc acagcgttgt ttgcatttaa
    15961 agatttaatg tggccattga ttgtcaatac ttcaattgat aagatgcctt tagctccggg
    16021 attagcaaac ttacaaggac aatatgccac gaacttccca caattaatgg caggttcgtt
    16081 attagcaatt tggccaatag ttgtgatatt catcgttttc caaagaaaat tcattgaagg
    16141 aattgctaca acaggtagta aattataaaa ctattaatta gtgattggag agtaagtaca
    16201 tgacaaaaat tacatttatt ggtgcaggaa gtactatttt tgcaaaaaac gtattaggcg
    16261 atgcgatgtt aactccaagt ctacaagatg cacatattgc attgtacgat attgatgaaa
    16321 aacgactaaa agaatcaaat ttaatgctta aaaatattaa taaaaattcg aatgaaaatc
    16381 gtgctgatat tgtttcttat tatgatcgca aagaagcgtt aagagaggct gattttgtta
    16441 tcaatgctat tcaagtgggt ggttataaac cttcaacagt gattgatttt gatattccta
    16501 aaaaatatgg gttgcgacaa actattggtg acaccatggg gattggtggg atttttagaa
    16561 ccctacggac actaccagta atgttagact ttgctaaaga aatggaagaa gtttgtccaa
    16621 atgcttggtt attgaactat acaaatccga tggcgatgtt aaccatgggt gttttaaaag
    16681 cgacgagtat taagactgtt ggtttatgtc atagcgttca agtatgcgta ccagaattgt
    16741 ttgagcattt aggaatagcc gatcaatatg acttaaatga ctttcaatgg aaaattgcag
    16801 gcattaatca tatggcattc ttgttggaaa ttacaaaaaa tggggaagat ttctatccaa
    16861 caattcgtca gttagctgca gaaaaaccaa atccacatcc agattctgta cgttttgaat
    16921 tgttgaaacg ttttggttac tatattacag aatcaagtga acacaatgca gaataccatc
    16981 catactttat taaatcaaaa tacccagaat tgattgacga attagggatt ccgttggatg
    17041 aatacttacg tcgttgtgaa caacaaatta atgattggga aagcatgcga gaggacattg
    17101 taaataatgg ggagttaaca catacgagaa gccgtgaata tgcttcatat atcatggatt
    17161 ctattacgac aggaactccg tcagtgattg caggaaatgt cttaaataaa ggcttaatca
    17221 caaacttacc gacagatgtt tgtgtggaag taccttgttt agtcgataaa aatggcgtgc
    17281 aaccaaccta tgtcggtgaa ttgccaacac aattagctgc tttaaatcgg acgaatatca
    17341 atgttcaaga actgacagta gaagccgctt taacactaga taaatcaaaa atttatcaag
    17401 cagcgtattt agatccacat ctatcatctg aattgtcttt aacagatatc aaaaatttag
    17461 tggatgattt aatcgaagca catggggact atttacctga atataattaa aaagaagctg
    17521 aaagagtaag gaattttcaa ttccttactc ttttcttagt taaatttagt aaaaatattg
    17581 acaaaacact tagtaaacag ttacattagt gttaacaaaa agaaaacgca ttcaaattgt
    17641 ataaggagga gcataaaatg aagtggtcta aaaaaattat tttagcttgt atagcactag
    17701 gagcaacgat ctctttgact gcttgtggaa aaaatcagga agatgcaagc ggtaaggtag
    17761 aaatagagtt ttttaatcaa aaaaaagaaa tgactcaaac gattcaggag attgctaaag
    17821 attttgaagc aaaaaaccct gatattcatg taaaagttgt ggatgtaccg aatgctggtg
    17881 aagtaattaa aacacgtatg cttgcaggcg atgtaccaga tgtcattaat ctttatcctc
    17941 aaagtataga attgaaagaa tgggcgaaag ctggttattt agaggactta accgaagaac
    18001 cgtatttaga gaatatcaaa aatggatatg cacaacgttt tgctattgag gatcgtgtat
    18061 atagcatacc attaaccgct aatgtctatg gtttttacta taacaaaact gcttttgagg
    18121 aaatggggat tcaagcacca gaaacttggg cagaatttga aaaaatagta gctgagatta
    18181 aagatcaaaa caaagtaccg tttgcgattg caggagcaga aggctggact ttaaatggtt
    18241 atcaccaact agctatagcg actgctgctg gtggtgaaga ggaagcgaac aatgtttggc
    18301 gtttctcaga agttaatgga attaatgctg aaagcaaaga aatgcaaaaa gattttaatc
    18361 gattagattt gttaagagaa ccaaaagctt tgcaaaacaa ctggcaaggt gccggttata
    18421 acgatacagt agttactttt acaaaaggtg atgcgttgat tatgccgaat ggttcatggg
    18481 caatgccgat gattcattca caaaatccag attttgaagt aggaactttt ccttttccag
    18541 cagatgaagc aggtcaaagt ctaacgattg gcgcaggaga cctagcctta tcagtttctg
    18601 caacttctca acataaagaa gaagccaaac gttttgtaga atatatgaca acacctgaag
    18661 cgatgcagaa atattatgat gtagatggtg caccgtgtgc tgttgagggc gttatcgaga
    18721 atactcagga ttcaccatta agtggtttaa ctgaacttgc ttttactgat cgtcatttag
    18781 tttggttagc caaagattgg aatagtgaaa atgattttta taccttaaca acaaattatt
    18841 tacacaatgg caatcaaaaa gccatgatta acgcattaaa tgctttcttt aatccaatga
    18901 aagctgacgg aaggtaggaa aataaaatga aaataaaaga aattttaaat cgttactggg
    18961 caatcatttt tgtagcaatt ccaattattt tgcagatgat ttttttctac tttccaatgg
    19021 ttcaaggtgc tttttacagt ttaactaatt ggactggtct gacttataat tttgattttg
    19081 tgggtttgaa caattataag attctttttt ctgatccgaa gtttatcaaa tcgattggct
    19141 tcactttaat tgtgacgctt tgcatgattg ctggacaaat tggttttggt attttgattg
    19201 cgcgggcgtt gaacgcaaat attaaaggga aaacattctt tagagcatgg ttcttttttc
    19261 cagcggtctt atcgggcgtg gttgtttctt tagtatttaa acaaattttt aactatggtt
    19321 taccaaaaat tggtagtatg ttaggcattg aatttttaca aaccagtctt ttaggaacaa
    19381 catggggagc gatttttgca gtaatttttg ttttactttg gcaaggaatt gccatgccag
    19441 tcatcatttt cttagcgggt tttcaaagta ttccacaaga aattattgaa gctgctagaa
    19501 tggatggggc gaatgataag caaatttttt ggaatattga aatcacctac ttattaccaa
    19561 gtatttcaat ggtatttatt atggcattaa aaggtggctt aacagcattt gatcaaattt
    19621 ttgctttaac tggtggtgga ccaagcgatg ccacaacatc tttggggtta cttgtttata
    19681 attatgcttt taaaaacaat cagtttggtt atgcgaatgc tattgcttta gtcttattta
    19741 tcttaattgg gattgtttca ctggcacaat taaaattatc gaaaaaattt gaaatctagg
    19801 aggtttccca atgaaaacat caaaagaaaa aaatatttct aaatacttgc ttttattatt
    19861 agggggcttc atgattttag ttcccttagt ttcaacgcta ttcggttcat ttaaaacaac
    19921 acccgatatt atgaataatt tctttcgttt tcctaatcca gcgactttgg aaaatttccg
    19981 aagattgtta gcggatggaa ttggtcatta cttttggaat tcggcaatca tcacaatcat
    20041 tgcagtagtt gctgtgacag caattattcc tatggctgct tattcactag caagaaaaat
    20101 gactcgtggg aaagcatacg gattgatgta tttattttta attttaggaa tttttgttcc
    20161 tttccaagta attatgattc cgattaccgt tatgatgagt aaaattggtt tagcaaatat
    20221 gtggggatta atcttactct atctcactta tgccgtacca cagacactat ttttatatgt
    20281 tggttttatt aaactaaatg tgcctatcag tctggatgag gctgctgaaa ttgatggggc
    20341 tagtcacttt acaacttatt tcaaaattgt tttcccaatg ttaaaaccaa tgcatgcgac
    20401 gactattatt cttaatgctc tatggttttg gaatgatttc atgctaccat tattaatttt
    20461 aaatcgtgat tctaatatgt ggacactacc attgtttcaa tataattata cgggacaata
    20521 ttttaacgac tatggtccaa gttttgcttc ctatacagta ggaattgtca cgattacact
    20581 agtttatctt gctttccaaa gaagtattat agctggaatg agcaatggag cagtgaaata
    20641 accaataaat tattctcatt ataaaggagt tcataaactt atgattatat ttgacaatca
    20701 cacacaaaca tttcatttac aaaataaaaa aattagctat ctattaggaa tcgaagaaaa
    20761 taattattta acgcatcttt attttggaaa aaagattacg cactattctg gtgggtatcg
    20821 ctacccccga acagatcgtt ccttctcacc caacaatgca aatgcgcaag atcgtttatt
    20881 atctttagac acattaccat tagaatttcc aggatatgga catggtgatt ttagagaacc
    20941 ggcgcataat attaaattag gaaacggtag tcgtgtaaat gattttcgtt atgatagcta
    21001 cgaaattcaa aaaggaaagc ccagcttagc gggattgcca gccttatata cggaagatga
    21061 tgatgaagct gaaacgctga ttattcaatt gaaagacgcg attagtggat tgactttaca
    21121 gttgttctac acaatttatc aaaatcacag tgtaattatt cgttctacgc aattagtaaa
    21181 taatagtgat gaaacagtgg agattaaccg cttagctagt caatcaattg attttccaaa
    21241 tcgtccactc gagttaattc atttaaatgg tacctgggca agagaacgtc aaatgactag
    21301 agaaaaaatt catacaggga taaaagtatt agatagtaaa cgcggatcaa gcagccatca
    21361 acaaaatccg tttgttgctt tagtagatgc caaaacagat gaatttcaag gagaggcata
    21421 tggatttaat ttagtttata gcggtaatca cgaaacggtg attcaaaaag atccatttga
    21481 ccaaacacgg gtcattatgg gaattaattc atttaatttt ggttggcaac taaaaactgg
    21541 cgactcattt caaacgccag aagtcgtgat ggtttatagt aatgaaggtc tgaatacgtt
    21601 atcacagacg tatcatgaat tgtataatca ccgtttaatt cgtggaaact atcgtttaaa
    21661 agaacgtccc acgttaatca acaactggga agcaacgtat tttgatttta acgaagaaaa
    21721 aatcatgaat attgtcgatg atgcgaagaa cttgggcatt gaaatgtttg ttttagatga
    21781 tggttggttt ggcggtcgtg aaaatgactt cacttcatta ggtgattggg ttgaaacaga
    21841 aggcaaactt gcttgtggtt tggaaaattt agccaagcaa gtgcatgata acgggatgaa
    21901 atttgggatt tggtttgaac cggaaatgat ttctgaagat agtgaactat ttcgagcaca
    21961 tccagattgg gcattgggta ttccaggtcg tgaccgctca attagtcgaa atcaatatgt
    22021 tcttgatttt tcaagacaag atgttcgtga taatatctat caacaaatga cggcgatttt
    22081 tgatcgtgta ccgattgatt atgtgaagtg ggatatgaat cgaaatatga cagaagtcta
    22141 ttctgtcttg ttggactcaa cgatgcaagg tgaagtggct catcgttata tgttagggtt
    22201 atatgaattt ttagaaaagt taacaacgaa ttatccaact attttatttg aaagttgttc
    22261 gggtggaggc ggtcgttttg atgcaggttt cttgtattac atgccacaaa cttggacgag
    22321 cgacaatacc gatgccgtgg ctcgtctgaa aattcaatat ggaaccagct tagtgtatcc
    22381 aatttcaagt atgggtgcgc atgtttcagc agtcccaaat catcaaacag gaagagaaac
    22441 gagtttaact attcgtggca atgctgcgat ggctggtgtt tttggttatg agctagatct
    22501 aacgcaatta acttctgaag aaaaagcaga aattaaagaa caagttgcct tttataaaaa
    22561 gcatcgccaa cttttgcaat atggaacttt ttatcgcttg gaaagtccgt ttgaaggcaa
    22621 tgatactgca tggatgtttg tctcaaaaga ccaaacagaa gccatggtct tttatttccg
    22681 agtattagca gaagctgcgc atccattagt cacattgaaa ttagcaggat tagcagaaga
    22741 agcaacttat caaatggaag atgtaacatt tgctggcgat gaattgatga atctcggttt
    22801 ttatattgac tcagaactac atggtgatta tgcgacacaa cgcttttatt tgaaacagat
    22861 atagtagctt gaatttacaa tataagtgca acaccaaaag agactcattc caaacgaaga
    22921 aaatatgttt tatttacacg catttactaa aaagcagcct gatttgaatt gggaaaatga
    22981 agaagtacgt gaagaaatct atgcaatgat taactattgg ttagataaag gactaggcgg
    23041 atttagaatc gatgcgatat taaacatcaa aaaaaaacta gagtatggca tctttgaatc
    23101 tgatggagaa gatgggttag ccttcattgg gccctggatt cttaatcagt cgggcattga
    23161 agtgtggtta aaagaaatga gagagcggac atttaaacca cataatagta tgacggtagc
    23221 agaagccgat gttcctaatg aacgtttaga tgaatatatt ggtgaagatg gctattattc
    23281 gatggttttt gattttagtt atacggatat tgatgtacct gaaactggtg aatggtttaa
    23341 agatagccaa tggacatgga cggatatgcg taccaatatt tttacgaatc aattagttac
    23401 gcaagataaa ggttggggcg ccttatattt agaaaaccac gatcaaccac gttcaattaa
    23461 taaatatatt ccagaagaat ggatcaatga ttatagtaaa aaaatgttgg cgacgctttt
    23521 tatgttacta agaggtaccc cttttattta tcaaggacaa gaactaggaa tgaccaacat
    23581 agaaatggca tcattagaag attttgatga tgtagctacg cattctcagt ataaacgggc
    23641 attagaatat ggattggcac cagagcaagc attgaaagct gtcaataaac gaagtcggga
    23701 taattctcgc acacctatgc aatggaatac gcaaaaaaat gccagattct caacttctga
    23761 taatgtatgg ttaaaagtaa atcctaatta tccagaactc aatgcagaag ctcagttaac
    23821 caatcaacag tcggtattca attattatca tcaattaatt aaattaagaa aaaatagtgt
    23881 atacaaagac gtgctaatct acggacaatt tctaccagtg aaagataaaa atgaacatat
    23941 tttgctttat gaacgccaat tagaagataa aatcgtatta gttatcttaa acgttacagc
    24001 aacagaacaa gaatattatg tagatgaaca gtaccaacaa gtattaatca ataattatga
    24061 agatgtttta ttagatcaaa gtcgaaaatt gcgtttacga ccgtttgaaa gtattgtatt
    24121 agctaattat ggaggacaat tatgaaaatt aaaaacgaag caatgctcat cacctatgca
    24181 gatagtttag gaaaaaacat gggcgaatta aaacaagtct tagacaaaca tttacaaggt
    24241 gttattggtg gtgtacatct gttgcctttt tttccatcga caggtgatcg aggctttgcg
    24301 ccgagtgatt atacgacagt tgatccagct cttggaacat gggaagatgt ggaagcatta
    24361 ggtgaagatt attatttgat gtttgatttt atgattaatc acatttctcg tgaatcaaaa
    24421 tttttccaag atttcaaaaa aaaccatgag cagtcaccat acaaagaaat gtttatccgt
    24481 attcatgaat ttttcccaga aaatcgacca acacaagaag atattgattt aatctacaag
    24541 cgtaaagaca aagcgccgtt tcaaacggta gaatttgcgg atggtacgac agaagaggtt
    24601 tggaatacct ttggggaaga acaaattgat ttagatgtga ccaaagaagt tgtgaaagaa
    24661 tttattcgag aaaccatcaa agatatggcg aaccatggct gttctttaat tcgattagat
    24721 gcgtttgctt atgcgattaa aaaattagat accaatgatt tcttcgtgga accagatatt
    24781 tgggaacttt tagacgaagt gcgtcaagaa gcagcgaaat atgacgtcga attattacca
    24841 gaaatccatg agcattattc cattcaaatg aaaattgcca atcatgatta ttatatctat
    24901 gacttcgcct tgccaatggt cactctgtat tcactttata gtggaaaatc agaacgttta
    24961 gccaattggt tgaaaatgag tccgatgaag cagtttacaa cattggatac gcatgatgga
    25021 attggtgttg tggacgctcg tgatttatta acagatgaag aattggatta cacctctgag
    25081 gaattgtata aagtcggggc gaatgtgaag aaggtctatt caagtgccaa ttacaataat
    25141 ttagatattt atcaaattaa cagcacctat tatagtgcat taggcgataa tgaccgtagc
    25201 tatctgttag cacgtgcgat tcaagtattt gcgccaggga ttccacaaat ttattacgtt
    25261 gggttattag caggaaaaaa tgatattgaa ctgttggaag caacgaaaga agggcgtaat
    25321 attaatcgtc attactatga tttagccgaa attgaagaag aagtgcaacg gccagtgatt
    25381 caacaattat ttgcactttt aaaatttaga aatgaatctg cggcgtttga tttggatgga
    25441 acgattgaag tagcaacgcc atcagaatca gaaatagtga ttacacgtaa aaataaaaac
    25501 ggtagtgata gtgcaacatt aacggctaac ttggatacga aagaatttat cattgagtct
    25561 aaagatataa aaattttcac tagtgttgca ttaaaatttt aacctattcc atagaacaaa
    25621 aaatccttta tactagcact tggatgactt atccaagacc aataaaaagg actcatacat
    25681 ggataagtat aaaacaaata cagcatttaa taaatggttt tctgccataa aattaggaaa
    25741 actttctgtc aactcccaaa aatgtatttt tgattacaat tcgtatcgta aaaaactgtc
    25801 ttttgaagct gtgctcaagc tctttctcta cgcagttaat gaagaaaaag aaagtttgcg
    25861 tgatttgagt acagctctta tcgaccgatc gctccaacta gaaaccgatg tgtcatcgat
    25921 tagtcatacc caattgtctc gagcatttaa tgcacttgaa cctaaagtgt tagaagaaat
    25981 ttttcaacaa ctgttagaaa aagttcaaca tcaaatcaaa ccaaccaagc gaaatcatct
    26041 gtatctgatt gattccagta cgttttcgct tagtttaaaa cgtcataagt gggcgacttt
    26101 ccgtcaaaca aaatcaggtg tcaagttaca cttaaactat tgttatatgg acgatactac
    26161 gatgtatcca actgatttta ccttaacaaa tgcccatgaa catgatctca atcaactacc
    26221 agtattggtg aatcaaccag aggcaactta cgtgtttgat cgtgggtatt aggactttga
    26281 aaagatggat cagatgcatt gggatggcta tttcttcgtc actcggatta aaaaaaacac
    26341 caaagttcac gtgatagata ctctggaaac atcgccagaa acagaaattc tacgagacga
    26401 gctggttcgt ttaggatcta aaacctatct aaccgccaat ttccgactag tcactgttca
    26461 agataagaac ggacgtgtgt ttcaatttat caccaatcga atggacgtct cttcaaaaga
    26521 aatctctgat atgtatcacg ctcgttggca aatcgaattg tttttcaagc acatcaaaca
    26581 acacatgacc attaaaacct tcttttctca aagtgaaaaa ggcgtgcaga atcaattgat
    26641 tttaacgatg atttccgctc tcctcacgtt tctcatcaaa ttggagacga aaacggaaaa
    26701 atcagtgttc caaatcaaac gattctttcg ttatttactg tttcagccct ttgaatgttg
    26761 tctagaaaaa ttaattccca catgagaaaa aagaaatttg gctcgttact tttggttttc
    26821 agttgaataa cagataaaaa attttctgga aaaagtcatc ctctgcgtca gcacagtttt
    26881 ttttgactta ttttcagagg aaatagtttt cagaatattc taaaatgttt tatgcaacac
    26941 tagtgatatt ttttagaatt agatttctaa tgtgcatttt ttgagtatta aggagcaagt
    27001 atacaaagat ttataaagct gattaaattt gaataaaaat atgcaggaac gctactattt
    27061 ttagtagtgt tcttttttat atttgaataa aaaatgtggt ggttaaatgg tggttataac
    27121 aaaaaatgaa ttaatgaaat taggttatgg gaagtatcag tctgagagaa tcatacgaca
    27181 ggcaaaagca attatggctt caaaaagcta tacctattac gaaaatagaa gattaggact
    27241 agtacctgtt gaatctgtag aagaagtttt aggtatgaag atagaggtag aggaggattt
    27301 tcagaatcat ggttaatttt aaaaaagata aaaatgggaa atatcatttt actgctagtt
    27361 tgggattcga caaacgaaca gggaaaagag tacaaaaaaa cgtagagggg ttaaaacagt
    27421 taaagaagaa aaaaagcgta taccgaactt gtttctaatt atggaaaaga agcagaaact
    27481 tctcaaagta ctatgttgtt tgaggagttt ttttataaat tattcctacc ttattatcaa
    27541 gggaaagtag aagaaagaac atataaaaat cggttatcta caatcaaaaa aacttttctt
    27601 atttctttaa aatggagtta agagaaatac gtccaattga cattcaaaag tggcaaaata
    27661 atttattgag caaatataaa aattcttatg taagaaacat gtatgggatt tttcagatgt
    27721 cattagatcg agcagttgtt ttgggactac ttgaaaaaaa tcctgcaaaa gttattggaa
    27781 atgtgaagaa aaggaaaact acagttgatt tttggacaaa agaagaattt acaaaagtta
    27841 tttcaacttt ttatattgat gatttgtacc aacattttgg ttttatttgt atgtggttac
    27901 tttttatgac cgttatgcgt ttgggagaag ccactgcatt aacttggaaa gatattgatt
    27961 ttgaaaataa aaacttgcgt gtgaataaaa cactctatta taagagagcc aaaacctatg
    28021 agtttgtaga acccgaaacg gcttctagta ttcgtacaat cgaactagac gacaatacaa
    28081 ttaccttttt aaaagcttgg aaggagcgtc aagtttcaat aggaggcatg tcgtttgtac
    28141 tttcttataa tggaacacct actcagaaat atacaatccg tcatattatg agaagacatg
    28201 caacgatcgt tggtgttcat ataatccgca tacacggatt aaggcatagt catgcttctt
    28261 tgcttatatc acaataaaaa gatgcacttc gaattaaaga aaggttaaaa catgctgatg
    28321 tacaaacgac tttaggaact tatggtcatt tgtatctaaa ttcattgaga gagataacaa
    28381 atgatcccac agattatatc caaactaatt tttctaaaat caaaccgaca cctatttacg
    28441 ataataaaaa ataaatgata aacaaaagta ggatcacacc aaaagaaaca cactagaaaa
    28501 actataatac aggcttttct agcgtgttgt ttcttatttc cgcctattta tacattatac
    28561 tcataaagca tttaatgcat cgatcgtagc cgcattgtct atatgggtat ataaagttgt
    28621 tgtattggta cttgaatgtc ccaattgttg ggcagttaat accaatgaat ttgtctgttg
    28681 gtagagtcta gtagctaatg tatgacgcag tttgtgaggg ctgacttgaa ttctaaagcc
    28741 ttttgaatat tttgctacca tccgttcgat tgcaataccg gatatacgct tggcttctcc
    28801 ttggtaaatt gctagaaata aagctttttc gttttctgtt actttatatc gttctttccg
    28861 aatagctaga taattagcta aatattccat tgcgaatgct gctatattga cagcatctct
    28921 tttatttcct tttctagtta ctaccacatt attcttatct aaagataggt catctaaatt
    28981 aatatttata acctcggaga gtctcaatcc agatcctaaa atcagtgcat tgatcgccag
    29041 gtctctttct acatctcgtt gataatagat caaagctcgt ttactcaaag tatttttata
    29101 ttcattggca atataatcta gataattttg tgtatcctta tctagaaata attttttctc
    29161 aatggcattg gcacgtgcag ctaacgtagt tttatctgtg atggttgcta cttgatccat
    29221 aggattattg tctaagtatg gttttcctag aggattattg gaacttttcg aaagatattt
    29281 aaatagaacc tttaatgccg tgatatttct attgatagta gaagtagata gacctaagtt
    29341 attattatct ttgagcttag tttttgttgt ctcttttttt acattttctt ttgtgcgacg
    29401 tttcaaatga gatttgtaca agtttaggtc ataggcagtt aactgtctta tcgaggtgat
    29461 agaagtatca ggaacgatag actcactcag aagccattca taaaagagct tataattttt
    29521 tagatattct aaagtggttc gaggtgattg atctttatcc tcacgatctt gaacaaaatc
    29581 ttttatataa tcgggcatat gaagtatgat tgtatctatt ttttgatata tttctgtatt
    29641 tttcatgtca ttcatccttt caataagtag gggagaagag tatctctata attggactat
    29701 accaattata gaatgttttg tcaattggta tgctctaaaa tttattaatt tgtttcaaaa
    29761 aaagcatata aacaggtata cgtttaataa tttaaaataa atggtataca taaataggga
    29821 aggaaataga agggagcaat ctaaattttt tgaattttct taattaatta tatagaaaga
    29881 acaatctttc tatagagtta gacatgagag ggactatgct tgatttaaaa tcaacaagcg
    29941 tatatactgt atatacaaag aaaaaggagg gagaagacta tggcaattaa agaaagaaaa
    30001 tttagggaag tggggaactc agtagtatta actatttcaa aggagtttct taaagaaaat
    30061 gggttaaatc ccggagatac ggttttttta gatgaagaaa aacttcgagc ggcaattaca
    30121 aaaaaacaag ccttatctga tgaaattgat ttagcaatat ctcaagcatt aactgaatat
    30181 gatactactt ttagagagct tgtcgataaa tgagtattca ttacctagaa gccaaagata
    30241 ttataaaaat gaatgtaatc caaataaaaa aatactctcc taatgaaccc cttggtgtaa
    30301 aggatgcgaa tgctttagaa atgtgtgtag ggcaacttcg agcctcagct tttggtgaag
    30361 aggtttatcc ttcgatttat gaaaaagcag ctatcctact tatacaattg ataaagaaac
    30421 atccttttca caatgcaaat aaacgtactg ctttcttagc tacttttgtc tttttgaaaa
    30481 tcaatggaca gcttttagct attgatcaaa aagatgctgt aaatttagcg gtatatattg
    30541 ctacgtatga taaggatttc gatcaactaa aatatgaagt gatcaacact ataaagacac
    30601 atacgcagcc catttaagga gttactgtat gtgtctttat agggttatta gctgctatag
    30661 cgaaataatt gaagtaaatt acaaatagct ataattcaaa tccaactata tagaaaacgt
    30721 aaattttcta tatagttgga ttaagataac gactatttat tttatcatat ttttactata
    30781 ccctatactt ttacttggta tatttatgaa taatcgcaaa tttaccccta tactactagt
    30841 ttgtaagtta gattaataaa aatactaaag ttatttgttc ttttattaaa tacacctaca
    30901 acttataaat tgatggtatt tagataacac gaaagaataa aaataacagc tgatagtaaa
    30961 gtttgttgac gattgctact caatcgaaag tgagttagtg cgtatgaact tgcttctgaa
    31021 aacttatgaa atgaaagacc ttttacatct tgcagaaatc gttactggga ttaaactaag
    31081 caataaaaac taatgttccc ctcattcttt gaacttctac tatttgattt atttattctg
    31141 ataaatgacg atttcacctt caggatcttc aaatcccgta tagaatttat ttaagatctc
    31201 ttcagtatca gtaataggat aggcgatagg ccacataccc tttgatggtt agaataaact
    31261 gctcaaaatc tgtatcgtat gttggcttta gcatattaac aaaggctatc tccaaattat
    31321 aaaccataag cgttgaatcg ttcttaacaa tttcaattct tcctaaccca aagtcagaag
    31381 atataattac cggacttgcc ttacccttca tgtgggttgt tgagattcca tatttaaagg
    31441 gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct
    31501 gctagtctcc taagtccctt tgtagcggat atggcaccat aatctcattt tatcgaagtg
    31561 agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc accctctacg
    31621 gcattctagg ggagtgcact tgcattcacc caataaaaac cggcagttag caaagaactt
    31681 atgtagtcgt atgttcttac tgttaacata caagaggtct cctttccaca attgcttagc
    31741 agtaaacggg aatttacaaa gctaatctct tccattttct tatctttgtt ctaaaagtcc
    31801 caaatggagc aactgtattt acatgaataa atttatatac ttcccataca gccgttttgg
    31861 ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag atcttcatca ctcattgaat
    31921 caatcatcat gttaatatca tcaatattac gattgagaat ttcctctaat tctttcaatg
    31981 gtagatgtgc ataggtattt gtaaaccact tatataactc tcctaactga ttccatttaa
    32041 attgatcaga aggcgtttta acagataatc catttctttc atcattttcc cattttagaa
    32101 ttaaattcgt ccaaccaact tgatatgcta aattttcagc aggtgttcta tccacttcct
    32161 caactctttt atccttcaat tcttcaggaa tagtttcaaa ttctaaaata tatttagtat
    32221 aagtcctatt tatttctttt tttaattctt ctttattttt atatgttctc attatattac
    32281 ctccataaat tcctaattgt ctcctttaat tcatctataa tttcatccac gatattttaa
    32341 tccgttctac aatgcatatc tgatatgcat tgactgtcta actcatatgt gaatgattta
    32401 taaaatatcc caacgtctca tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata
    32461 cgaatatatt tcttttgtag taaaatcgga ctggtttgca tatcattttc ttcgtaaata
    32521 tcaaaatcat gttaactact atcaattact aataaaaact accactttaa gcgttaagaa
    32581 ctgttaagtt tagttaagtg aggatagttc acaaaatcca ttgttaaact tcacgaaagt
    32641 ggtaaaactc aaaatgcttt atataggatt acagcatcgc tctttcttcg attgcgcgtt
    32701 gggtaaaaaa tattatgaag taaaaattga cgatgacacg ttccttacgg ctcatcagat
    32761 gaaacagcta caaaaacgaa atgatcaact cgaagaaaat ttaattctaa aaaagcgatt
    32821 gccatattca cgcctcgctc gacgaactat taaaggtcaa gatttacctg aagatcatcc
    32881 tttttaggaa attgaagctt tttatgttgc acaatgcatt tataatacta caatgatgtt
    32941 ttcaccagat attcttctag ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat
    33001 tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat cttccgaaat ttttttccaa
    33061 aattaaggga tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt
    33121 ttaaacaatt atagaaaacc gatactctgt taagttggtt gacacggtgt cgatttttat
    33181 gactttctta ttctgaaact tcactaacgg acagtaccta aaaagtctat agtatggacg
    33241 tttacgagta ctattttatc aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact
    33301 acccccactt agcttggata ggctacaatt aactagacag aaaaattaag gtgtgtagac
    33361 tagaagaaaa cataccagga ggaattttta tgtctaagag aacacgaaga actttttcac
    33421 aagaattcaa gcaacaaatc gtcaatcttt acttagctgg aaagccacgt gtagaaatca
    33481 ttcgagaata tgaactaac
//
LOCUS       DAYYFD010000023        33261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000023 DAYYFD010000000
VERSION     DAYYFD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.023"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHA25_001001"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHA25_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8762312.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACHA25_001002"
     CDS             500..862
                     /locus_tag="ACHA25_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8762313.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACHA25_001003"
     CDS             914..2596
                     /locus_tag="ACHA25_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8762314.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACHA25_001004"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACHA25_001004"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8762315.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACHA25_001005"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACHA25_001005"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8762316.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACHA25_001006"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACHA25_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8762317.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="ACHA25_001007"
     CDS             6431..7435
                     /locus_tag="ACHA25_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762318.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="ACHA25_001008"
     CDS             7436..8380
                     /locus_tag="ACHA25_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762319.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /gene="opp4B"
                     /locus_tag="ACHA25_001009"
     CDS             8380..9342
                     /gene="opp4B"
                     /locus_tag="ACHA25_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8762320.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="ACHA25_001010"
     CDS             9369..10283
                     /locus_tag="ACHA25_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762321.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="ACHA25_001011"
     CDS             10309..12090
                     /locus_tag="ACHA25_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8762322.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /gene="rnc"
                     /locus_tag="ACHA25_001012"
     CDS             12438..13124
                     /gene="rnc"
                     /locus_tag="ACHA25_001012"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8762323.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="ACHA25_001013"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="ACHA25_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8762324.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="ACHA25_001014"
     CDS             16734..17543
                     /locus_tag="ACHA25_001014"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8762325.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="ACHA25_001015"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="ACHA25_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8762326.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="ACHA25_001016"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="ACHA25_001016"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8762327.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="ACHA25_001017"
     CDS             complement(21095..21547)
                     /locus_tag="ACHA25_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8762328.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="ACHA25_001018"
     CDS             21697..22647
                     /locus_tag="ACHA25_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8762329.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="ACHA25_001019"
     CDS             22640..23599
                     /locus_tag="ACHA25_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8762330.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="ACHA25_001020"
     CDS             23596..24351
                     /locus_tag="ACHA25_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762331.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="ACHA25_001021"
     CDS             24374..25327
                     /locus_tag="ACHA25_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8762332.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="ACHA25_001022"
     CDS             complement(25971..26267)
                     /locus_tag="ACHA25_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8762333.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="ACHA25_001023"
     CDS             26622..26978
                     /locus_tag="ACHA25_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8762334.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="ACHA25_001024"
     CDS             26988..27887
                     /locus_tag="ACHA25_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8762335.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28407..29135
                     /locus_tag="ACHA25_001025"
     CDS             28407..29135
                     /locus_tag="ACHA25_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8762336.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="ACHA25_001026"
     CDS             29280..31307
                     /locus_tag="ACHA25_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8762337.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..32512
                     /locus_tag="ACHA25_001027"
     CDS             31415..32512
                     /locus_tag="ACHA25_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8762338.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32819..>33261
                     /locus_tag="ACHA25_001028"
     CDS             32819..>33261
                     /locus_tag="ACHA25_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762339.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKR"
BASE COUNT        11138 a         5762 c         7123 g         9238 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
    32521 taacaaatag taaattttta ttattgtagg atggttgggc tctttttata acaatataaa
    32581 atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta ataacaggtt
    32641 tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat
    32701 gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta
    32761 tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat
    32821 gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt
    32881 agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc
    32941 gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct
    33001 agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa
    33061 atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt
    33121 attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt
    33181 gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa aaaaatatca
    33241 aatttttttt gaaaagagaa a
//
LOCUS       DAYYFD010000024        32837 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000024 DAYYFD010000000
VERSION     DAYYFD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.024"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..2731
                     /gene="clpB"
                     /locus_tag="ACHA25_001029"
     CDS             122..2731
                     /gene="clpB"
                     /locus_tag="ACHA25_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8762340.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2882..3568)
                     /locus_tag="ACHA25_001030"
     CDS             complement(2882..3568)
                     /locus_tag="ACHA25_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762341.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3546..4040)
                     /locus_tag="ACHA25_001031"
     CDS             complement(3546..4040)
                     /locus_tag="ACHA25_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8762342.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4060..5280)
                     /locus_tag="ACHA25_001032"
     CDS             complement(4060..5280)
                     /locus_tag="ACHA25_001032"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8762343.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5427..7262
                     /gene="lepA"
                     /locus_tag="ACHA25_001033"
     CDS             5427..7262
                     /gene="lepA"
                     /locus_tag="ACHA25_001033"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8762344.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7458..8369
                     /locus_tag="ACHA25_001034"
     CDS             7458..8369
                     /locus_tag="ACHA25_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762345.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9380..9616
                     /locus_tag="ACHA25_001035"
     CDS             9380..9616
                     /locus_tag="ACHA25_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762346.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9729..9971
                     /locus_tag="ACHA25_001036"
                     /pseudo
     CDS             9729..9971
                     /locus_tag="ACHA25_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10061..10300
                     /locus_tag="ACHA25_001037"
     CDS             10061..10300
                     /locus_tag="ACHA25_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762347.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10447..14730
                     /locus_tag="ACHA25_001038"
     CDS             10447..14730
                     /locus_tag="ACHA25_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8762348.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14829..15194
                     /locus_tag="ACHA25_001039"
     CDS             14829..15194
                     /locus_tag="ACHA25_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762349.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15196..15648
                     /locus_tag="ACHA25_001040"
     CDS             15196..15648
                     /locus_tag="ACHA25_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762350.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16097..17572
                     /locus_tag="ACHA25_001041"
     CDS             16097..17572
                     /locus_tag="ACHA25_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8762351.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17595..17906
                     /locus_tag="ACHA25_001042"
     CDS             17595..17906
                     /locus_tag="ACHA25_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762352.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18266..19522
                     /locus_tag="ACHA25_001043"
     CDS             18266..19522
                     /locus_tag="ACHA25_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8762353.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19519..20064
                     /locus_tag="ACHA25_001044"
     CDS             19519..20064
                     /locus_tag="ACHA25_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762354.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20065..20565
                     /locus_tag="ACHA25_001045"
     CDS             20065..20565
                     /locus_tag="ACHA25_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762355.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20924..21202
                     /locus_tag="ACHA25_001046"
     CDS             20924..21202
                     /locus_tag="ACHA25_001046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762356.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            21222..21566
                     /locus_tag="ACHA25_001047"
     CDS             21222..21566
                     /locus_tag="ACHA25_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762357.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21585..22496
                     /locus_tag="ACHA25_001048"
     CDS             21585..22496
                     /locus_tag="ACHA25_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8762358.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22575..23567
                     /locus_tag="ACHA25_001049"
     CDS             22575..23567
                     /locus_tag="ACHA25_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8762359.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23564..23782
                     /locus_tag="ACHA25_001050"
     CDS             23564..23782
                     /locus_tag="ACHA25_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8762360.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            23795..24205
                     /locus_tag="ACHA25_001051"
     CDS             23795..24205
                     /locus_tag="ACHA25_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8762361.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            24257..26785
                     /locus_tag="ACHA25_001052"
     CDS             24257..26785
                     /locus_tag="ACHA25_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8762362.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            26801..29011
                     /locus_tag="ACHA25_001053"
     CDS             26801..29011
                     /locus_tag="ACHA25_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8762363.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            29019..30110
                     /locus_tag="ACHA25_001054"
     CDS             29019..30110
                     /locus_tag="ACHA25_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8762364.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            30131..30673
                     /locus_tag="ACHA25_001055"
     CDS             30131..30673
                     /locus_tag="ACHA25_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8762365.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            30868..31125
                     /locus_tag="ACHA25_001056"
     CDS             30868..31125
                     /locus_tag="ACHA25_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762366.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            31139..31471
                     /locus_tag="ACHA25_001057"
     CDS             31139..31471
                     /locus_tag="ACHA25_001057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762367.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            31516..32064
                     /locus_tag="ACHA25_001058"
     CDS             31516..32064
                     /locus_tag="ACHA25_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8762368.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            32071..32523
                     /locus_tag="ACHA25_001059"
     CDS             32071..32523
                     /locus_tag="ACHA25_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762369.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            32620..>32837
                     /locus_tag="ACHA25_001060"
     CDS             32620..>32837
                     /locus_tag="ACHA25_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762370.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGIS"
BASE COUNT        11226 a         5048 c         6959 g         9604 t
ORIGIN      
        1 gagaaaaaaa gtcaaaaaac tatgtgtata ttttgaccaa aattgaccaa agcgatataa
       61 tagtttttgt aaagatcaaa gaaataagtg atcttacctt taattaggag ggtatacctg
      121 tatgaatatt gagaaaatga ctacgacgct tcaagaagcg attgctgaag ctcaacagat
      181 tgcggtcact cgaaaacatc aagatatcga cattgcccat gtatggaaaa tctttttaca
      241 atctaatcat tttggtcgaa atttctatac ggatgccgga ttggatgtcg aatcttttga
      301 acatgaaatt gatcgtctac tggatgagta tccagttgtt tctggtggaa acgtccaata
      361 tggacaaaac ctcagccaga atttctttcg attattgaat gaagcagacc aaatacgtga
      421 aagcttcggc gacgagtttc tttcaacgga agtcgttatt ttagcgttga tgaaattaaa
      481 aaattatccg ttaactgttt atttgaataa aaacggatta tctgaaaaag aattgcgtaa
      541 aaacatcgaa gagatgagag gaggagaaag agtgacctca caaaaccaag aagaacaata
      601 caaagcatta gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga
      661 tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac
      721 aaaaaataat cctgttctga taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg
      781 gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt
      841 ttcattagat atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag
      901 actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga
      961 tgaaatccac aatatcgtag gggcaggaaa aacagaagga agtatggatg ccggaaatct
     1021 gttgaaacca atgcttgctc gcggtgaatt acatttgatc ggtgccacaa ctttagatga
     1081 gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt cgctttcaaa aggtattagt
     1141 caaagaacca acagtggaag atacgatcag tattctgcgt ggattaaaag aacgttttga
     1201 gatccaccat ggtgttaaca tccatgacaa cgcattagta gcagcagcta ctttatctga
     1261 ccgttatatt actgatcggt tcttaccgga caaagcaatc gatttgatcg atgaagccag
     1321 tgcaacgatt cgggtagaaa tgaactctat gccaacggaa ttagatcaag ttactagacg
     1381 tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa
     1441 aaaacgcttg aaaaatttgc aagaagaatt agctgaactt cgtgaagaag caaatgcgat
     1501 gaagatgcag tgggaaacag aaaaagaaga agtcaattct gtttcagcaa aacgtgctga
     1561 aatcgataaa gcaaaacacg aattagaaga tgcagaaaat aactatgatt tagaacgcgc
     1621 tgctgtattg cgtcatggta cgatacctca attagaaaaa gaattgaagg aattagaagc
     1681 taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa atgagattgc
     1741 tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga
     1801 aaaactaatt aaattgaatg aaacacttca taaacgagtg attggtcaag atgaagcagt
     1861 tgatgcggtc agtgacgcgg taattcgttc acgggctgga ttgcaagacc caaatcgtcc
     1921 tctaggttct ttccttttcc taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc
     1981 gttagctgag aacttgtttg actcagaaga tcatatggta cggatcgata tgagtgaata
     2041 tatggaaaaa catgctgtgt ctcgtctagt cggagctcct ccaggctatg taggttacga
     2101 agaaggtggt caattaacgg aagctgtcag acgaaatcct tatacaatcg tcttgttgga
     2161 tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc tagatgacgg
     2221 acgcttgact gattcaaaag ggcgagtagt agatttcaaa aatacggttc tgatcatgac
     2281 cagcaatatc ggttcacagt tactgctgga aggcgtgact gctgatggaa cgattccaga
     2341 agcagtggct gaacaagtca acacgctatt acgtggaaac ttcaaaccag aattcttgaa
     2401 ccgaatcgac gacacgattt tatttacacc attgagccta gacaacgtga aaggaatcgt
     2461 tgacaaaatg gtggcgcaat tggcacaacg tctggaacat caagaaatcc tgctgacaat
     2521 cagtgatgaa gccaaaacgt ggattgctga aaatgcttat gaaccagcat atggtgcaag
     2581 accattgaaa cgatttatca ctaaggaagt agaaacacca ctagctaaag aaatcgttgc
     2641 aggacatgtt atgcctaaat caaaagtaac gattacttta cttgatggac aacttcattt
     2701 caaaacagaa gagctagaag aaatcgtcta atcttccatt gagactataa aagaaaaaag
     2761 gggtctgaca caactagtgt cagacctcct tttttcaaat aaacagtgtt ccagaagtag
     2821 tttcttcgaa aaataagccg aaatttcttg aagctgcccg cttctgtcac aacctctttt
     2881 attaggtatt tttcactttt tctaatttat aactttcaaa gtaacgctca tcttttttac
     2941 tttgagacca tgcaaacaat cccattaagg cagcacctat cgtatttgtc agcaagtcgc
     3001 ccatcgtatc catcaatgct gctcgtccga caaacagtgt gccatcagaa gcagcaaaac
     3061 gttgaagatt catgcctaag aactggtcgc ataagaattc ccagaactcc caaaacacgc
     3121 cgcaaagtcc agcaaacgca aagccgaata agaggaacaa ccaaggactg gtttttgata
     3181 tctcagcatc tttcatcaag taaccaatca atccatagcc aagagctgtc aggaccattg
     3241 gactcaccgc atgtaagatt ttgtcccaaa aagagatgat actgatcaaa tgcatccctg
     3301 tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa caaaacgatt gctgctggta
     3361 ttttaagttt gaaaactttc aaaatgactt gtggcatgaa aatcaatgca atccctaata
     3421 aagattccac gataaatgtg attccttgag aagtcgaata tttttgtgtg ataaattcat
     3481 aaaaattata gcagacaacg aatacaccaa aagcaagtaa agctatttgc acctttttga
     3541 tttctttaat cactgaaagt ccctccatct aaaaatcttt ttacttctga aagcaactca
     3601 atttctgaag aaaccacttt tccgttcaat ttgatcaaac ctattgtata taagttcagg
     3661 tagtgaaatt gattttcagc tgtttcttgt agtgcagcga tttttttcgg gtgggttccg
     3721 ccttgctgtc gagtatcagt atataagccg atcactggga tgcctttcgc ataagccaca
     3781 ccgatttccg atgctacccc ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa
     3841 tcactagcca acactttttc tgtgtcagca agagcaatca ttcgactgtc tgcataagca
     3901 gttttgtcat tgattgcttc attttcttga ggaagataga tcgaaagctc agaagagagc
     3961 tctctgattt ctttaactaa ctgttggtta tataacaaat cacttttagc aaacattggt
     4021 gcggcaaaat aaattttcat ctaaacatca tcctttttat caaaaataat cgctaaagta
     4081 agcttccatg tttggaatga tacgctctag tgacttcagt gtttcttcat ctgtttctaa
     4141 ccgttcgatt cttgccaaat aagaagcacg gcgataattc atcatcaagc aagtcaatgt
     4201 ttggatatcc agtcgaacgg attttcctat aggttcattt aagacacgga cttgtccctg
     4261 ttcatcccat gttaaagcga agacaccatt attccaagga gcgacaggat cttcaatgat
     4321 gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc
     4381 aacgatccga gccataaaat atggttcgat ctgctctttg atctgactat cttcaatcaa
     4441 aaatgctaaa ggctcatttt tataaatatc tccatgtacc cagtagatca tggaaaaatg
     4501 tgcggaaata aagttccata agccatttcg agcttcttga tcaagataga acatttcttt
     4561 tacgtgaaat atttcttcag ctacccagta gaacagaaca cctcttggtt catggttgct
     4621 atcgtagtag acagcggctg tgcgctcatc ttcattttcg aaacgccaat actcttccca
     4681 atgaaaatca ctgcggaata aagcaccatg gttttgttgg gcaaatttgt cataaacgga
     4741 aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata ccacttactg ggatttgttt
     4801 aggtagctgt gtatctctga ttttaaagga aagtttatca gacatgattt cccatccttt
     4861 acgccgatag taggggatac tgtaaggata caaataagag atccattgct tattcttgcg
     4921 catattatcc aatgcaacga taattagatc ttgcatcaat ccatggttag catattctgg
     4981 ataagttcca actccagtca caccacccat tttataacgt gcaccgtgaa tattgacttc
     5041 acaaggatag atagcgattt gagagatcag tttattttcg tggaaccatc cgaacacttt
     5101 cgatacttcc aaaatcggtt ttttggaacg gataaaagct cgtttattct caaacccgct
     5161 ttcctcgatg tctgcttcag tcacttggaa cacataggaa agcaattcat tgaattgatc
     5221 gatatgttct tgagtgaccg gttttaattc aagattttct ctaaaatctt tttgatccat
     5281 tttttcatct ccatttatta gcttgataac agtatagcaa aattctacaa aaaaaacttc
     5341 tatagctgac taattaaaaa ctctttaaat tactgctttt cgttggtata atggaaagtg
     5401 ctgattatat gaaaagagga gacgctatgg atataaagaa aatgaaagaa cgccaagagc
     5461 agattcgtaa tttttcgatc atcgctcata tcgaccatgg gaaatctaca ttggccgatc
     5521 gtattttaga aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag ctgttagact
     5581 cgatggattt ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta gaactcaatt
     5641 acacagcgaa aaatggagaa acatatatct ttcacttgat tgacacccca gggcacgtcg
     5701 acttcaccta tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg
     5761 atgcagcaca aggaatcgaa gcacaaacat tggccaatgt ttatttagcg ttagacaatg
     5821 atttggagat cctgccagtc atcaataaaa tcgacctacc cgcagcagat cccgaacgtg
     5881 tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag tgaagctgta ttagccagtg
     5941 ccaaatcagg gattgggatt gaagatattc tcgagcagat cgtggaatat gttcctgcac
     6001 caagtggaga cttagatgca ccgctaaaag ctttgatctt tgactctatt tatgatagtt
     6061 accgcggtgt tgttctaaat gtccggatca cggacggtat ggtcaaaccc ggagacaaaa
     6121 tcaaactgat gagtaatggc aaaacatttg atgttacaga ggtcggggtc ttctcgccaa
     6181 aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca
     6241 aaactgtcca agatacacga gtaggggata cagtaacgtt agcagacaat cctgccgctg
     6301 aagctttacc aggttaccga aaaatgaatc cgatggttta ttgtggtctt tatccaattg
     6361 atacttctcg ttacaacgat ttgcgggaag ctttagaaaa actgcaattg aatgatgctg
     6421 ctttacaatt cgaaccagaa acctctcaag cattaggttt tgggttccgc tgtggattcc
     6481 tcggacttct tcatatggat gtcgtccaag aacgcttgga acgggaattc aatctagaat
     6541 tgatcacgac tgcaccatcc gttatttacc atgttaataa aacggatggt acgactgcta
     6601 cagtagataa cccggcagat tttcctgaac ctgtaacgat tcaggatgta gaagaaccat
     6661 tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg tgcagtaatg gaactttctc
     6721 aacgaaaacg gggagaattc attacgatgg attatttaga tgattaccga gtaaatgtcg
     6781 tctataatat tccgctttct gaaattgtct ttgacttttt cgacaaattg aaatcaagca
     6841 cgaaaggcta tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc aagttagtga
     6901 agatggacat cttgctgaat ggagaaaaag tcgatgcatt aagctttatc gttcaccgag
     6961 actttgccta tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc
     7021 aacaatttga ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc cgttcagaca
     7081 tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt tctcgtaaac
     7141 gtaaactctt agagaaacaa aaagaaggga agaagcggat gaaacaaatc ggctccgtcg
     7201 aagttccgca agaagccttc atggccgttc tgaaaatgga tgagcaagat agtgtgaaat
     7261 aacttataat caatcgaaaa acctattaaa taaacctttt tgagtagatg caacactgag
     7321 gattatctgt ataaaagatt ttaaactgtc atttggttat cacggcgata ctaaaattaa
     7381 caaaattgga atatttttct tgaagatttt tagctgtcag tatatgatga aagcatctca
     7441 taggaggtgc ttttttcatg gttgataatt caagtggaag ggttcttaaa tctctaagaa
     7501 aagagaaaaa actatctcaa aaaaagttag ctgatttagc aggtatttca caaagtactt
     7561 tagttaaata tgaaaaaggg tcaagaaaaa tccctaaaga cgtagataat actctaagca
     7621 agattctgaa tatagaaact ctaataaaag atgaggaaga taagattgag attctcatcg
     7681 gtaaactaat tgcatacaga gatatgaata agttacttaa caaagagtta gctgataata
     7741 taggaattag tgaagtatta ctcagttatg tgttaaatag aaagagaaat ccgtcaaaag
     7801 aaatgcaaaa aaaaatcgat atttttctat taagtaatga aaaagaaatt ttgaaggaga
     7861 ttaatcgtga tagtgagata tttagtttat caaaagacga taaaattgtt atgggaaaaa
     7921 gaatacgaga agtccgaaag aaccgggaag agacgcttga aaaatttggt aaaaacttta
     7981 ccatctatac aggcaaaaat gttataagta gatgggaaaa aggtattaat attccagata
     8041 ttgaaaaact aatgaatatt gcctatcttg gtaaggtcac tgttccttac cttatgtacg
     8101 gagaagatta taagaatata cttccaaaag atgaaagagt tagcgatttt aaaaagataa
     8161 attcttttag tatgggatta aggatgagga aaattaggaa agattattat ctagaaaggg
     8221 aggagtttgg aaagcttttt tctccttcaa tttcaaaatg gtccatagat aggtatgaga
     8281 atggtagaga tatacccaat actaatcgaa ttatccaata tgcttatata ggaaatcttt
     8341 ctttagaatt tttgatttac gggatataaa aatagtttac gtcgatactt attaagttat
     8401 cgacgttttt tattatagaa aaaatttata aaaggaggtt tcttgatatg ataagaaaca
     8461 aaaagggttt gttgaaagtt gctttactgg cagctttttt ctaagtaact ttttgacaag
     8521 ctcaatggca acacaagcgg aagaagtaat acaagaacta actgttatga ataacagcta
     8581 gataaaatgg gctatgtatg ttatccaaca atttagtaat cactagaagc ctagcgaaag
     8641 ttcgcttgat aagttataga tgagtattga tttgtagtgt ttccaaatca aaagggtaca
     8701 gagccacatg aaatatgttg tcctctgcag cgtcaggatg ggaatccata ccatggagat
     8761 tcggagatct gtgagggaat acttacgtgg ttaatatgtc agttgtgttg gacgtagtaa
     8821 tggtcatagt gggtagattg actgaattct tatatgagaa aatgaaacca acaagaagat
     8881 agggttattt atacagaatt ggggatcttt tctggaaaag taatcggata atgcgagatt
     8941 cgtacggtct tttttaaatt tctctaaaaa aaacggcatc gaatgggttg ggtgattgat
     9001 aagcaatgaa tcatagactg atatagaata aaaaatgatt ctatttatct ttttacatct
     9061 atcgtttttt caagtagccg caaattatat cagcattcaa caatttttgt atacaagttc
     9121 tagtaaaagg atctgaagaa aaagttggtt gttgtgaaga taaaaaaaga atcaggtttt
     9181 actgagtttt tcttcaaaat ttgtgttatt aggttaggac aaagataaga agaagtatga
     9241 agatttaaaa aaggaatgag gaaaagatag taatttccga ttattggagt ttttttattt
     9301 ataaagaaga agcgaataag ttgtgattcc tagatttcgc gcgtgattaa ttaatgagca
     9361 aaaaatgcgg aaagttttag tgtttaatac acgaggacaa agtttcttag gtattgaagg
     9421 cacacgtgta acggttgatg ggtggtgtga ttgtttcgag gttggaaaac atgttgaact
     9481 tatggcattt actgagctaa aagataagaa caacgtggaa tttttcgaga gtgatattgg
     9541 ttgggacgat catgaagaag tttatggaaa atttattttg cggaagggaa attcttatac
     9601 gaatggggaa gcataagtag cgatttgtta gagatcattg ataggattga aattgtcggg
     9661 aatatttatg agaatctaga gttatttgag gtgatagaat gagtgatggt tttgaacaag
     9721 tatcaataat taaaaatcaa gttcgggaga ttcttagaaa aaaaggctat ttggtagata
     9781 gttattttga aggagattac gaaacatggg taggtgtcta tgctcgtcca gagaataaac
     9841 ctacctattt agatcctacg acgtcagaag atgcctattt acaaaatagg tatcgagtag
     9901 atggttttaa gcaagacttt gcggagtggt ttgaatggga aattgaaaac ggagaagtaa
     9961 aagaagaata agagtacttg ttgtatcaaa ataagccagt agaacaacca aaccagtagc
    10021 tagtaatgac aataatactg aggaatcgga tcattatggt atgattgata tttctcttcc
    10081 agaagatctt aagccagatc ctgtaacaat tggcaatagt gtcatgattt ttgattcatt
    10141 agatgaagct atgtcatatg gttggaaaga aattaaggat gaaggtagta attggtatgg
    10201 catgagattt ggtgatggtg aagttgccaa aaaagctggt attccaattg ggaaatggac
    10261 agttgatttc cacgaagcag gttctttttt tgttctgtaa ttatagtggg agggtaaaat
    10321 cattgatgaa atgtgttcag ataagagcgc cagataaaaa tagaagagac agttcacgtc
    10381 gttatcactt ttggtaacgg cgttttgttt tattatataa aattctcagg aaaaggagga
    10441 attcttgtgt atgcaaaaac gaaaatgaat tggttgaaag cactcgcatt ggtggctttt
    10501 tctcaattct gtttcttggt gggaactgct caagcacatg cagaagaaac gacggctata
    10561 caacctacga tggaagccat tgcccaagtc gcttctgaag tgacagaagt ccaaccagca
    10621 gaagccgggg tcccagctgg tgaagccact gttgaacagg cccctgtgac agaagcgcca
    10681 acggtcaatt ccaaaacgac tctcgatcag acgattgggg aaacacaaca cccagggaat
    10741 agtcctggta cttcacaacc agttgaagaa actgcctcaa ttacaacccc tgcttcagaa
    10801 acgtccactc tagaaggaga aaaagctgcc gaagcaacgc cttctgtgca gccagagtct
    10861 tctgagaaac aagcggaaga aaagaaacga atagaagaaa cagtggaaca accagcggct
    10921 actgctgaca aagaaggaaa aaatgaggaa aacgttgaaa aaacaatctc ttcaaccgca
    10981 gctagtcaaa atcaaccaac gaaaccaaac aaagaatcta cggctagtat tgaaaatcaa
    11041 aagaaaaaag agcagaacat tccaaaaaca ggtacaccta cactgttagt aacagtacca
    11101 accaatcgtt cggttcgtgt tcgccgtgca accaatgcag ctgttttagg tggtcggaaa
    11161 attgattggg gcgaaaatat tggtgttact tctagtccga acaatctaaa cttcacttta
    11221 aattatatgg ttcgttttga gattaatgga caagtggcgt tttgtattga gcctggtgtc
    11281 tcagccgcag aaggtcaagt ttatacagct actgctttga aaaactattt gaaaaattca
    11341 aatgtacgga aaaaaacaag cttgatctct tatttaggat atgtcacaaa taaagacaaa
    11401 tccgatgaac aatacattgc ggcacaattt atgatttggg aagctttggg cacaaaaatc
    11461 ccaatcaaaa atatccgtgt tgattatgat cgtcgaaaag cagaaattca acgtttgatt
    11521 aataactttg gtgaaaaagc aacttttaat aatcaaacac atacggtaaa aattggcgaa
    11581 acgttgaaag taaagaataa aaatgatttt ttgaaatatg tgaaagacat tcaaaaacca
    11641 aaaggtgtca atgtatctat tcacggcaat gaagtatgga ttacagtcac aaaagaggca
    11701 ccggataaag caaaaattca attgaataaa attaaagttc aaggtgcgcc attagcctat
    11761 cgtcgtccag gagctcaaac aattggtgtg ttacatccaa cagaacctaa tgcgtctgtt
    11821 attaatttac aaattctaaa acaaggaaac attcgtgtgt ttaaagaaga tgcggaaact
    11881 ggtgtgaaag cacaaggtgc agcttcttta gaaaatgcgg tctatggttt gtatcaagca
    11941 gatgggaaaa agattaaaga aattaccttg aaaaaaatta acggcaaagt acaggcagaa
    12001 attaaagatc ttgatcctgg caaatatgtt ttaaaagaaa tcaaagcgcc aaaaggttat
    12061 gtgctttctg atgaagcaat tgaaattgtt gttgaacctg gaaaaactgt caatgcgacc
    12121 gcaaaagatc aagtgatcaa aggaaccatt gatttagtga aagttgctaa taaagattta
    12181 gtcgattcaa cgaatccaga taacaaacca aaattagctg gcattgaaat cactttgacg
    12241 tcaaaaacaa ctggcaaagt ggtgaaaacc gttgtgacag acaatgatgg ttatgctact
    12301 tttggtaaaa ataccgttgt ctttgatacg tacattcttt ctgaaaccaa aggaaaagaa
    12361 gggtataaac tatttgaacc acttgaagtc acaatttctg aaaatgggca aactttccat
    12421 tatgttttag aagataaagt aattgaacaa cgtcttaaag tggtgaaagt ggatcaagaa
    12481 acaggaaaag tgattccact tgctaataca gaattcaaga tttttgatac tttagccaat
    12541 cgttatgtca caatggagat tccaaatgat acagaaacaa cggatatctt taaaaccaat
    12601 gaaaaaggct ttttcctaac taacggtaca cttacttatg gaaaagatcg ctatcgtttg
    12661 gaagaaatca aagcccccgt aaattacgtt ttgaatcaaa acccattagt gttctcgatt
    12721 accaatgata cagaaccgat caaagtgatc cattttgcga accgtcaagc acggaaaagt
    12781 gtaaaattat tcaagtatga agaatggaac aagaagaaaa cgccgctcgc aggtgtgaac
    12841 tttaccttgt ataacggtaa aaatcaagca attggtgaat ataccactga tcaaaacggg
    12901 gaaattttag tgaaaggtct agttgccgat gactattact tcttggaaaa tgccccatta
    12961 gaaagtttcc aaccaaatac agataaacaa gcattcacgg ttgcattcaa acaaaatgcg
    13021 ccagatgatg gggaaacgtt agtggtttct gtacgtaatt acttgattcc accaaaacta
    13081 gaaacacaag cgtcaaataa agccgatggt gaaaaagtgg ttgatccatt agaaacgatc
    13141 gtcattaaag acagtgtttc ttataccaac ttgtttgtcg gaaaagaata caccatttca
    13201 ggagtattga tgaacaaggc aacgaatgcg ccaattttac aagatggcaa agaaatcaca
    13261 agtaccttgg cctttattgc agacaaagtc aatggcacaa aagaccttga atttgtggtt
    13321 aatgctagtg ttctgaaagg tcaaacgatc gttgtttttg aaaaactttt ccgtgatggc
    13381 attgaagttg cagcacatgc ggatatcaac gatgaagccc aaagtgtgaa agtgaataat
    13441 ccaaccgttc ataccacggc tgtctttgaa gacggattga cagtcgcaga cccaacagga
    13501 ttgtttacct taaacgatac cgttgaaatt accgagattc ttccaggaaa agcatacttg
    13561 gtcaaaggga tcttaatgga taaagcaacc ggtgaaccct tgcttgtgca tggaaatacg
    13621 gtagaaacga gtgttggctt tattgccaaa gcagaaaatg aaacggtgat cgtaccattt
    13681 acctttgatt tgacaggtat aaatggtcgt gaaatcgtcg tgtttgaaag tctttttaga
    13741 aatgatgaag aactagcaaa ccataaagac atcaacgaca aagggcaaac ggtacgagta
    13801 actaacccaa gtgtacgtac aacggctaca cataaaccca ctggcttgaa agaggttaat
    13861 ccattaacga aagtcaccat tacagacgct gtgaaatacc acgatttggt cgttggaaaa
    13921 acatacacag tcaacggtgt tctaatggac aaagtaacgg aaaaaccagt gctagtagat
    13981 ggaaaagaag tcacaagtac cttaacgttt gtggcagaaa caacggatgg tacagtcaat
    14041 ctagacttta ttctagacgc acgtgcgttg cgaggcaaag aagtcgtggt ctttgaagat
    14101 ctgtttagag aaggcgtatt attggcttct cacgcagata ttaccgacaa tgaccaaacc
    14161 atcaaaatca gtaatccaac cattacgacg aaagcgacga ataaaaatgg cggaaaagag
    14221 atcttggcct tgccaaatca aactgtgctt gaatgggtca aagtagacgg tcttgcgatt
    14281 ggtcaaatct atcgcttaat tgttcaaggc tacttaacac cagacggcac accatttgaa
    14341 ggcactcatg cggaaaaagt ctttgcggcc gataaagaaa tgatggaatt cttatttgaa
    14401 ttcttagtgg acggtcgcaa tcttgcagga aaaggcttga gttttgcgga atggctccaa
    14461 gcgaaaacgg aagatgagaa agaaacgttt gaagaagtgg cgaaacacaa tgtagatcta
    14521 acaaacaaag atcaaacggt tcaaattgtg gaagcgccaa aaccaccgca accagtgaaa
    14581 ccagaacaac gggttaaacc agctactgtt gtaccaatgt tgccaaaaac aggtgaacaa
    14641 acaagtagta tacttatggt tcttggatta gtagtattag tcattgcttt aggtataaca
    14701 ctttataaag tacgtaaagc caaagattag tcatgaggaa caagcggtta ggatttttaa
    14761 ccgcttgttt gattgtaatg tagaaatatt tagaatttat tttggttttt aagaggagga
    14821 aaaactttat ggcagaaaaa gcattgcgta tgaagaaaga gtttttaggt caagtatttt
    14881 tattgggttc acgggaacct aatccaccaa aggaaattaa tggtgtgtct gggcgatatg
    14941 ttcaattaca agcagagtgt tcgcgagaag aacagcctgc tttttttgtt tatctcacag
    15001 ctgaagagga taaaagatta cgattaccag aaaaagaggt gcgtatttca tttgaagaag
    15061 cttattttgt ttttcgtaaa gaacgagtga atagtcgtga aacaaatgat atgggacaaa
    15121 cagagcgtcg aacaactggc tatcaagatg ttggtgtctt tttaactggt gtaaaaattg
    15181 gggaggtttt gtaagatggg attaaaattt ccaaataatt atgacattac agaagcatta
    15241 gatgaaactt gtttaggagc cgtacgcttt ggcgcattag aagagtctga tcgtgtattt
    15301 gataatgaaa caggcgatta tacgggagaa attcgatcac agcgagcaca attagttttt
    15361 ggaaatccag atgaaccaaa gattgcacga cgagttaaat ttccagcaga tgtagatttg
    15421 actggtttta aacgacgtga tcctgtgaaa ttaattgatc cagaatatca tgtggtatat
    15481 attccagcac aagatggcaa tcgtgaatat tcagatttag tgatttatgc gaaaggatta
    15541 gagaaagtag aagctccaac aaataatcct aatatgaaac aatcacaaga aaaaggttta
    15601 gatcaaaaac aaccaagtgt tgattctaag gaaaaagaga aaaaataagg tttttttgaa
    15661 agaataggaa ggagtcttcc tattcttttt atctaagata gaaggaaggg gagattttga
    15721 attcagataa aaccaatcaa atactttgag agattgtgct tgttatcaaa atatccaaca
    15781 attgattgag tattttggag acaatccaac gatttttgta caaacaaatg catatatttg
    15841 tgcggttatt gggcagtgta tgtttttacg tgatcttgtt cgtttttcat taccgtacca
    15901 gtggaaacaa gatcatcagg aagtgatttg gattcgtata gtacgaatat gtaatatgtg
    15961 tgcacaccct tataaaaatc gattaaatct ttttcgatta tgttttttta ctaaatgtga
    16021 tattcctgta ttagttgctt tttgtcaaaa gtttttagca caggaaaagt aaaaacagaa
    16081 aggaggaatg tgacaattga aagctcattt tttcaagtat cgtggtcggc gaattcgcta
    16141 tttcaatcgt tatttatggt tttgctatta tggtttcttt ttgtttccat ttttgattct
    16201 gttaggttgg tttagttgga aagtatttta tccacgttta agtacgtttt cgaattggca
    16261 aatgtatctt attcctggaa taagtattgt gttgtatttg ttgctggtta gtgggttaat
    16321 tttagggttg atgcattggt cgcgactaaa agaaggatat tttgcctctg tttattggcg
    16381 tcagttactt gttcgaatgt taattgacaa tcggtttgtt tatacaaaga aacaagttag
    16441 tgagaaacaa acaagggaaa aaatgagatt accaaaagtt tatttttatc aaaagaaaca
    16501 agaattagtg gtttcttttc ctttagatgg tgggcgtttt cacgaacgat ttttaaaaat
    16561 gggtcatttg ttatctgaag tatttttgcg tgatttaatt cgtgaagaac gagaaaaagc
    16621 ccgaatcaat tatgtattcc tttcagattc tggtcggatt cctattgatc aatgtatagc
    16681 agaaaatgga cgtattcatt taatgaaaac tttggattgg gtatatgatc aatcaccaaa
    16741 tatgttgatt gctggtgcaa tcggtggtgg gaaaacctat ttattgtatt ctttaattca
    16801 agcttgttta agtgttggga cagtagatat ttgcgatgga aaagcggcag atttagcagc
    16861 attaggtgac gtcgatgtat tcaaaggaca tgtgttttac aaaataaatg aagaaatgat
    16921 tatttgttta cgaaatgcat taaaagaaat gaatcaacgg tatgcctata tgaaaaccat
    16981 gaaagatcct aaatatgaac cagggaaaaa ttatgcttat tatgggttac caccgcattt
    17041 tatctttttt gatgaatggg cagcttttta tggttcgttg gatatggaag cgagaaatag
    17101 agtcgataaa ttgattcagc cattagtatt aaagggtcgt caagcaggga tgtacattat
    17161 tttagcaatg caacgaccag atgcagaata ttttccaagt ggtgttcggg ataatttaac
    17221 attccgtatt tctgttggtc gattgtctcc cattggttat ttaatgactt ttggagaaga
    17281 ttttaaaaat aagccatttt acaacaaaga tcaacctggt cgtggatatg cttttccagg
    17341 atttgatgta cctgtagagt tttttgcgcc atttgtttac aaagattatc actttatcaa
    17401 agaatttgag aaatttactg aaatgattgt ctatgatttt acagtaaaag aggatagtca
    17461 agatcaaacg atgggagatg ataatcagtt gttacaagaa caactagagc gccaaatgta
    17521 tttagcaaaa gatccaaaag gaacaagttt ttctgatgcg catattttgt aatcaactaa
    17581 ggaggtgaag ttaaatggca gagagttgtt attatagagt agctacagca atacgaatga
    17641 ttaatccttc tctttcttcc agaacgtttt atgattggtt gaatcgtatt gaacaagtaa
    17701 ctgattaccg ctttttgcgc aaagaacgtg tttttacagg aaaagttatt aatcaagtat
    17761 tgctaacgaa aaaagatata gaacgattga ctcgattgta tcattatcga gtagatttag
    17821 aagaggattt aactttgtca atttatcgcg ttttttctcc tgaaaaatat agtgaaataa
    17881 caaaattaga tcatttaatt ttgtagttat tatctatcgg gagggctcta gacgtagtct
    17941 ggtagccgca ctctgcctgg cggtgcgcat taaggctgcg aagcatgggc ctataatgcg
    18001 cactcgctag cagcgtgtgc agctcacaaa gaggtcgtgc gcttgcgcac ggcctctttg
    18061 tgtaaagccc cccgatatat aatatggggg gcagaaataa cataacacat agaaaaaagt
    18121 tttataaacc cttttgtatc aagtgattat ctaaggtcag cagttgtact acttttccca
    18181 aaaaacgagg gggactagtt gatatatcgg tgtttctaaa gggttaaaaa aatcgatttt
    18241 gttattttat aaaaggagcg agaaaatgac accgattgag ctaaaaagct acagaaagaa
    18301 gttaagtgca attcataata taagtggaga taaaaagccg ctatcacaag caagatttgc
    18361 gcaattgatg ggctgctcaa aagattacat tagaaagatg gaacaaggga aaagaaaaat
    18421 cccagaggat tttgaacaac gaatcaagga acgctttcga ttgtatatga aaagcggaat
    18481 agatcttcga gcaatgattg attatttgcg tctttccttt tttgggacag actacaaaac
    18541 agtgattcaa cgtgtattac gaatggatct gacagaattt gaaacacgtc caacgggctt
    18601 gtatcttttt gactgtcttt ctgttcgtgg gaatatctgg gtgttctggc attcaaaacg
    18661 aactgtaaca gaaaatgtac ttgtgcaatt ttcaggacaa ggttgccggg aatatgagga
    18721 agtcatgcgt gaacaacaaa cagattggcg aacatggcta tattccattt ggaaaaatca
    18781 aggaaagttg gaagctggtt atacacgtgt gcaatgtagc cgattcgata ttgcgttaga
    18841 tgaactttgg ggattggaaa cggaagaaca tttcgatctc tttgaattac tagaaaaaca
    18901 aaaagcaggt ttattagaaa tggactttaa gagattccag aatattggcg gttgtttttt
    18961 agatgatggc tattttcgaa gtgaaggatt atctttgtat tttggatcgc gaaagagtcc
    19021 tctctttttt aatttttatg agaaacgttt tgaaattgcg aaccgagaaa aaatttcaga
    19081 attagaagca ttaacgaaat atggtattta taatcgatat gagttacggt tagccaatga
    19141 gaaagcaaca caagcagtag aagcatttat tttaggtgat tcaccgaaac gtttaggaga
    19201 gattggcgtt ggtttaatta atgcaaaact tattgtttgg gatagactgg aaagtgatgc
    19261 acggattgtt gataaacgat gggcaaaatt atttcgttcg atggaagaat tggattttac
    19321 agtaaagcca gaaaaattct cattagatcg ttcagaacgc tggtttttga aacaagtagc
    19381 tccgacatta aagttgttac aagaagcaga tcgtatttta ggaagagact ttgtacaaga
    19441 agggattgat aatgccgaac taaatgacga gcaacaaaac tatctagaat ggatgggaac
    19501 gtcaggaggg aaaattgcat gaatgcatat acgacaacag aatggctgcg cctgttggat
    19561 ttaaaggctg caatcgaagc attaaatgaa aagatggttg atttgtctta ttttcgtttt
    19621 cgagttcctt atattgaaca agcggtaaaa gctggacgtt atcaggaaaa agagaactgg
    19681 caagaaattg cacgcttatt agaagttcgt aagggatacg aacaagaact cgaagagttg
    19741 gaatttagcc gaaggaaagg aaggctagaa ttcattcgct tttatcgatt ttcattgcca
    19801 atacctgcga ttttagcggt taaaaaaggc tgcgataaaa tgaagatata tgaaaactgt
    19861 gtggcagcac ttagcagcga aaaacctctt atggaagaaa tttcattggt gactgtcact
    19921 tgggaaatga gtcgccagcc aacagaagaa cagacatatc tttctttaga agagattgag
    19981 atagaattag aggaaattgg tcgctatgcc acttgttcta cctattgtgg atcggtcatt
    20041 agtattgctg gtgtgattgt ctaaatgaaa aaaattatga tttatgttgg tgcgtattgg
    20101 tcacgtgatc caacagtatt agaaaatcca gaggcggtac actattgctt gcgccaatta
    20161 ttctatctgt ataaagaacg attagaatcg ttgattcgtc agttaccata tactgacaga
    20221 cgtttagatg aacttcttct aaggtatcca gcaatgtata aacgacgaaa aaatagattg
    20281 ttaccagaag agtatcccat tgaaaaacat gaacttgaag ggcgatttgt ggcttatttc
    20341 tatgatgatg ttcgtatgcg cttagtagaa caacgaatgg aaatagaaaa tgatcgctac
    20401 tattttatca attattgtaa gaagcgtaag tatcaagtaa ctgatgattt ttatgattgt
    20461 attttacaag atggggaagt gattctatcc aaaattgctc caagttttcg tgaacttgtg
    20521 ccgattgatt ttttgaaaaa atgtcatttg cggattcttc cgtaaaaaag agctataaaa
    20581 ggaaagaaaa gtagaagcca aatcggtttc tgctttttta ttttttttag gaaggagaag
    20641 atggtatgtc actggaattt ttaggagagc caattgaaaa ggaagagttc gtttcgcatt
    20701 atatggattt gtttattcag gtgataaaaa actttgtgcg attgatgaat tttatccacg
    20761 cgaacaagac tatctagccg ctgaacgaat aaaaatcagt ttattatggg aaaaattcac
    20821 agatgaaaat aaccgaccgc cagattatcg ctatttatca gattgtttaa cgaataatgt
    20881 gctttctaaa gaatacctat ttcacaaacg tgagaaaaat caaatgtcgg ttgaacattt
    20941 ttgtgattgt tatattgcat tattgcgtag tcaacagtta ttaacagtag aacgatatgg
    21001 tgcagagttt ggttatgtct tgcaagctga gcgtcataga gctacaagat gctatgaaaa
    21061 agaagatcaa gtgattgatg gctatgaaga attatgtatt cgaaacaatc gctatttgca
    21121 acaacgttta tatgaacaat tgatgatgga tttttcaaat ctctatagag ggtatgaatg
    21181 ttcgatgatt caagaaaggt agaagaaaag ggggaatttt tatgtggcga ttattatttt
    21241 tggtggtatg tggtatggca ttgttctatg tgtatcgtgt ctatctctat ccacgaatgg
    21301 aacgatggac aagttggtat agagatcctt taaaacaacg aaaaggattg aagcggtatc
    21361 gttatgaatt acttgttgaa ttttcggatg ggcgacaagc acagtattat ttttttgcaa
    21421 actatccaaa atacaacacc agtgaactat tgattcaatt attcaaaaat aatccatatg
    21481 ttgggttaga agaaaacgga cgggagttct tttatcgttc agataatatt agtgcggcgt
    21541 ctatgaacaa aacacgaatt gcataaaaaa ttggaggaat aatcatgaaa aaaacaacat
    21601 tagcagtcgt aacaacacta ggaatttcat tattttcgtt tcaattaggg agttcattgg
    21661 tttatgcaga tgaaactgta gaaacaccta agtcagaaat tgtagaaaca ttgccagggg
    21721 aaggattacc agatgttgag acagagctaa atggaggaac aacggaaaaa ccagcagaac
    21781 ctgaacaatt gcagtcagag aaacctgaaa aagagccagt gacaccagaa ccagagtttc
    21841 ctgaatcaac tttaccgact gattcgatcg aagaagaagg tgtgcatagt tcaacaacaa
    21901 cggattcaag tatagtcaca actccggaac aatcaggaac aaccagttct tctgaaaaga
    21961 atactgattc gtcaagtacg gaacagccaa cgatgtcgtc taccagtgaa gaacaaaaag
    22021 aagaaccgaa acaacctgtt gaaaaatcag aagcaattcc aaaagaaaat aaacctattg
    22081 tttcagcaaa agaagtcaca gtatcggtaa ccccaagcgg tgaaattacg acgaatactt
    22141 ctcaaggaat gagtgtaccg attgtcacta gtaatgtaga ggaattgacc catattccaa
    22201 caccaaccac cccattaaaa gtagcaggtg atcaaacaat tgttggtgta aaagatggta
    22261 ttccattaat ccaagatagt gaagggaatt tggtaaagga tttgtcgatt cctgtaaaga
    22321 aacttccaag tggcaatatt gaaatcaaaa cagcagatgg aaaaacaaaa gtcttaccaa
    22381 agactggtga agaaatacat gttgcacttt ctgttctagg aggagtgttg acaagtattg
    22441 ctggatttgt tggatataaa agaaagagaa aaggaataga aaatgagggt aattgaatgg
    22501 actatttttc agaaaaacaa gcttatcgtt gtcaatcgta cgtactagta aaaacatact
    22561 agaaggagta gcttatgaaa aaacgacaac tacggcggaa agaaaaagtt tatacagaga
    22621 aagtaccgaa gcaaaagaaa atccattcaa aaaaagcagt gaaatggatt tggtgtttgg
    22681 ttttatttct ttgtttttca gggggcttgg cattttttca atccatgaaa acgaaggttc
    22741 aagtgtcgtt tttgaaagat gaaatccaac aattacaaaa gaaagaagta caaagtcggt
    22801 cagaattaac gtacacacct gaaattgaat cctttatgaa tcgttttgtt gctttgtata
    22861 tgaatacatt cgataatcat gaacaagaac aacaacgtca agaaacatta aaaaaagagt
    22921 tttatgcaca aaactttatt caagaaccag gaagtgtttc aggtgttcga aaattaactg
    22981 ctgcttcgtt tattggatta aaagaaattg aaaccattcc aactgctagt tataaagtaa
    23041 cttatgatat tcgctcgatg aatccagaga gtaagaaaga aacaagtgtg acaatcacac
    23101 aagtactcaa tcttcccttt tctatttcaa acaagggctg tcgtattatt gcttatcctt
    23161 attttacggc actaccaaag aatacagaaa aacaacgaat gattacttat gatgacacat
    23221 tatacgaaac ggttgatgcg tcggaagcga aagatgttga aggatttgtg gaagatttct
    23281 tagataagta tgcgaaaaac aatgcaaaag atatgtcata catgatgaaa gaacctgaag
    23341 gactcaatca aacagctaaa gttgatcaag tagagatgaa agtattgcgt tataaagaaa
    23401 cgaaaaaacc ttttattgta aaagcaacgg tcgtctttcg agatggtgat acgtcacttt
    23461 tacaaaaaga acaaatgacg attgtcatta cgaaaaaaac aaatcagtat tatgttgaaa
    23521 aattaacaca taattggttg gataggaaag gagaagaacg gaaatgacac tgaagggaat
    23581 tgtagatttt atattaacgc aaggtggcta ttttttaata atggtggttg tttatctggg
    23641 attgcgaaat tttatgaaat caaaagtagg aaatgcatta atatctatcg cagcaggtgc
    23701 cgcagtttat ttcttcttaa atgatccaac acgtgtattt gatgcgtttg gtaaaatcat
    23761 tgaaaaatta tttatggatt aggaggatag tgaagtggat tgggaacctt atgattattc
    23821 gattgtttat gcggaacctt atcgcgtaca acagattaca agcaattggc gtcttccatt
    23881 tccagttgca ttagtcaatg cagcaacttt tttgttggtc gtaatctttg aatatttatt
    23941 tttacgttca tttattggtt gggtcatttc tttttttcca ccagcaggag ttgtttttta
    24001 tataggactc ccctatttat gtgtgttagc actggcaaaa ctaaaacctg atggaaaaaa
    24061 agtgcatttt tatttatggg attggttttg ttatatgatt cgtatgatga atggtgattg
    24121 tgtgtattat aactgggaca aggtatcaaa caatgcattg gaagaattac tttttgtgaa
    24181 taagtatgtt tatttagagg attagaaaaa gggacaatca actggttgtt ccttttttat
    24241 tgggaggtga aattcattgg tgcttgtaaa agaaccagta aaagcattca agaaaaattg
    24301 tttgttaaat ggagaggatt gtatctgggc ctactatcat gtttttccac gaacaatttc
    24361 aaaaaacaca ccaaaaaaga cacaggaaat aaaagaaatg atggctcgtt tactgttgct
    24421 tttagcagat tatgaagatg tggagttggt gatgtatcca cgaacaatga atttacggac
    24481 gaagtttgac acattaggaa agggattttc tcccgatagt caagcaattg gtgcgtatta
    24541 tgcaaaagaa acagtgacat tgttggaacg agaaggtcaa gtctatcgtc cttcatttat
    24601 tttaggaatc aaagtcaaag aaaaagcaac ggtggagagt tttcatgatg taataaatct
    24661 aatctggtta gatttagcag gaagtttagc acgcttattt ggattagaag tagatgattt
    24721 ggaaactttg ttttctgaaa gtgatcctta tgtaaaagaa gtgacacaat tactttatag
    24781 cttttctatt cgtcccttag taaaaaaaga gctgttgtat ctcaatcgtc tgcaatatat
    24841 tcgaggaatg gctcacaaag tagaagaaca aaataattgg gaagtactga aagacaatca
    24901 aggaacgtat gtacgtatac cgaaggaaca taaaggcctt cttcaattag aagatgagtt
    24961 agggagtagt tatttagcat tgttgcctat tggggaaaca cctgaaaata tgagaaattg
    25021 tgatctattt caatttgctc aggatctttc tttcccagca gaattgcgaa tgaaaattca
    25081 ctatccacgc ttgaaaggac agggaggtat tggtttaaaa ctgaattggc tatcggaacg
    25141 gttcaaaaca gagcataagg aattggttca aaatgatgaa gaacctagtg aacgcttatt
    25201 aactgttcga ggattagtaa aacatttaaa gcaacgagta gataatgggc aacctgtcgt
    25261 cgattggatc gcatgtttta ttgtatacgg tcaaacggtt gaagaagttc gtcaaaatag
    25321 tgcaacggtt attcaacaac ttcatacgat tatccatatc caccgtgcaa aaaatgatca
    25381 acgagaattg ttctataaat gtttattagg gcaaccattg ggacggcagt tacattggaa
    25441 gcaccgaaca actgtagaag cagttggcga atggttgttt tcagcagtat cagaattagg
    25501 tatggaatcc ggctggtata ttggtcggca agatacatta ggggatggtg gttctcgagg
    25561 agaaaaaaca ccattagaag aattaattta tgcgtcaaat cgttttgtct ttttgaatct
    25621 tctagccatt gcggaaggaa tcaaaggtgc gttgtttgat gcgcctcatg tagcagtaac
    25681 tggaaaaacc ggtaaaggga agaccttttt agttggcttg ctctttttgt atagttcgtt
    25741 tatgaatgta caaagtttat ttgtggatcc caaaggagaa aagcgcaaat ggtttacgaa
    25801 actcattcaa gacccgtatt atcagaaaca ttaccctttg tttgtagaac atttgcaaaa
    25861 aatgagttat gtcacgttag atgctacaaa tccccgtaat tatggtgtat tagatccaat
    25921 tgtttgttta tcccgagtag atgcgaaaca agtagcccag gatatgattg atgagttaag
    25981 tccgttaggt accaatcacc ttttaaaagg ggcggtatta aagggaatcc aagaagtttt
    26041 agttcaaaaa gaagccggac aacaagttgg cttgatgcat gtggtcgact atttagaaca
    26101 aatggaacaa aaagaggttc gagaatatgg tgagttttta cgcttgacgg tagaagattc
    26161 gattttacgt ttaggatttt cttatggaga aaacccggga ttagacttta atgccaaaac
    26221 aacgatttta gagatccaag atttgaaatt accaaatgac aatgtccgac cagaactata
    26281 tacagatgcg gatcgaaagt ctttgtgttt gatgattagt ttaggtcgtt tttgtgaaat
    26341 gtttggaaaa cgagatagta gtaagaaaac agcgatttat tttacagaag catgggtatt
    26401 taataattcg aacgcaggac gtagtattat tgcagcaatg gcacgtgtgg ggcgttccca
    26461 gatgaatcaa ttagtgttag atacacaatt tattggtgac ttaggttcag ataaagaaaa
    26521 agggaatttt ggtgtcgtct ttgcttttga tgaagacagt gagcgagaaa agattttgaa
    26581 tcatttacgt ttggaagtaa atgaagcgaa cttagctgaa atgagccaca tgattaaagg
    26641 acagtgttgg atgttagatc cctatggacg tgtaggaaaa ctcaatgtac acagtttgtt
    26701 tgaagaaatg accgctgcat tacaaacaac ggaaaaaaca agtcactctc aagtagaaga
    26761 acaggatacg ttaatcatgc aatagaaatg aggtgaaaca atggcttata taaagcataa
    26821 ggaaaagaaa tggtgcgttt tgtttttttt aacgattgga ttcggactgc tttttgttgg
    26881 tggattggtg tatgcggcag aaaagcaggt ggatcaatcg atctatgaga ttgggaaatt
    26941 tcatattgat accttttctt cttatttatc tgatagtgaa ttttcattag gcaaaatgtt
    27001 atttggggca aatggcacag attttctaaa aatgatgacg aatatgttct ttagtttaag
    27061 taaattgatt tggcaaggcc ttgattttgt cattgagaag ctctatgaag gagctgcaat
    27121 gaacgagtta attcaccagt ttttcacttt ttcggatact atttattcaa aaatatacga
    27181 tcctattggt gcggggattg tcgtcttgta tgtgttatat ctatttactc ttcgtatatt
    27241 tagagggaaa cattacgcaa aaatggtgtt tattcgattt ctcttcgttg tcattttttc
    27301 aatggtttgg tttggctatg gtagtaacgt acctagtaaa ggagaagctt ttaccaaagg
    27361 gttaaatgat ttgtccgtgg aggtagaagg ctttattttt tctgctacaa atagtctaga
    27421 tgaattggcg acaaaagatg tcaatggtgt tttagtgcca acgacacaaa aggaagccat
    27481 tcaaaaagtg cgccaacttt attataaagc ggctgttgtt gatccgtata tattgttaaa
    27541 ttatgggaca acaaatttag aagaattgta tcaagcagag attgaaccac aagaatttct
    27601 agccaaggaa gcgtcaagtg aagaattatc tgatattgag acaaaagtga ccactgctgc
    27661 aggagaagat agagggaaaa agaaggctta tcgtgtctat attcaaccaa ctaaagctgt
    27721 ttataaagca gtcgttggtt ctatggtgcc attgcttaat ctaagtattg gattaccact
    27781 tgtagtgatt ggcatgattc gttttatctt tcaaatgttg gtgttgtgtt tattacttgt
    27841 gattcctttt tcattactcg cttcttttgt tccagtattt gattatatgc tcatgaatac
    27901 cttaaagtcc tttttgggct tgatgtttca aaaatctttt tacagtgtga ttattttggt
    27961 ggcatttttg atctataaca tagtagacga tttaattcca atgaatactg tttttggttt
    28021 tttaggaaat atgtttgtca aagcactcct atcaatttta ctcattgtaa aacggaaaga
    28081 tttattaaag aaactagggt taggagctgc agatcaaact ataaacaaca cgaaaaacat
    28141 agcaagtcat tctttgtctc aaatgaaaca actgcctggt caactatcaa ctgctggaca
    28201 aaatttggca atcaaaggtg caggattagc agggaatgtg tatcgagcaa cgaatccact
    28261 cgcttctgca gcgatacatg gatttcaaaa gtttcaacaa aaacgtacac cacaagaatc
    28321 ggtaaaaggg aggcaagtgg ttaatccctt tgaactaggt gggcgttcaa atcaagcctt
    28381 gccttatagt ggtcgtcatg ttcgttatca tggggtagag cctgaaatgt cttctaccaa
    28441 atcgaaaaca gagtggaatt ctggtctttc aaatggtttg attccaaatt cgtccatgaa
    28501 ttcagtaaga ccaaaacaac aggtacaatc attggcacaa ttgagtaagc aacaagggaa
    28561 taatcaaaga aaaccattgt taaataaagg tatcgctttt gttggtcaat cgattccgtt
    28621 gccttctgga aaagtacccg tgccacaatt aacagctgct ggttctcttt cacaacagat
    28681 tcgccaagtg aaaaatcagg cgaaaaatgg gagttatcca gcaatgcagc agttattgaa
    28741 ccaatactct gttcctaatt cgtcggtagc cactctatca ggaaatcgtt ctgtgcaaca
    28801 accggggcaa cgttcgtctg aaagtaaacc gtcgatttcc gcaccaaaat cgttacaacc
    28861 tgcattttct tctttagata agccaacaga gcaatcggaa aaaatagaaa aggatcctaa
    28921 aacactgccg attttggacg caaaaccagg aactgatttt actttaccaa aaggacgaac
    28981 agtccaaaaa ccattatcga agaaaggatg aggaagaaat gaagaaaggg tgtctaggtt
    29041 gtcttctttt ctttattctt cccttttttc tattgccatt ctttttttca ggagaacttt
    29101 ctgagcatgg aggagaagct acaggtctag ctgtcgcttt taatggggaa tataccgaag
    29161 gattaccgat tttttcagag attaaaggac gtggtccaat cacagatgaa catgcgcaag
    29221 cagcagttgg tgcagctgta aagtatcagt tattaccgag tgtgattctt tctcaattag
    29281 gctatgaatc agcttatgga cagtcacaat ctggaagaaa cgatacgaac ttttttggga
    29341 ttacctggtt tcctggttgt ccctacccac aaggttctgc acgtggaatt ggtggttctg
    29401 aaggtggttt ctatatgaag tttccgaact taaaagcttg ttatgactat tatggcttta
    29461 tggtggcgag ccaaagcaac tttaatcaat gtgtgggcaa taaagatcct ggaagttgtt
    29521 tattgattct aggtcgtgga ggctatgcgg cagcaggtat taccgaaggg agtccgtatt
    29581 atcgaggagc aatgagtatc ataacgtcct ataacttaac ggaatatgat acttttgcga
    29641 tcaagcattg gaatagccta ccaacaaaaa aaacttcgct tattgcagga aaaagtaaag
    29701 gcaatgcgaa ggttttggat cggatcattg gacgatccgt aaataatggg gaatgttatg
    29761 gtggtacggc atggtatgta tctcaattag gtggcccaca gttaatgggt tctggacaca
    29821 tgtttgcgat gttaataggg gaagattata actggacaac ctatggttgg caagtaatca
    29881 tgaacccaaa accaagtgat ttacaagcag gagatgtggt gaattggcaa gcaggtggtg
    29941 cattgtctcc tggaatctat ggtcacaccg gaattataac agatgtttca tatggcggac
    30001 aacatttctc tagtgtggaa cagaatgccg agcaaggacg gattatggct cggtatcaac
    30061 gcacgtacaa tcaaacaaaa attcgtagtc tggttcgtaa ggtgaaataa gaaaggcagt
    30121 gactagaaac gtgaaaaaag ggataatttt agtatttagt tggttatgtt tgataggtct
    30181 tactggttgt cattggtttt cttctgaaaa acaaaaaaca caattgagaa cgacagaaga
    30241 aacaatcgaa gcagtaaaag aaacaagtgg aactcttcaa agtactgaaa gtaacagtca
    30301 aattggttca ttagaagacg agacagcgca acaaatgcaa atagatgaaa tgacaaaaaa
    30361 tttcttaaaa gattttgcta gaaaatggag taatttcaca gatgtttata aacgcaatca
    30421 agctgtgagg gactatatga cagatcgttg tattaaagac aatagtattg atgtagaccc
    30481 gcatgtacaa atgaaaacaa ctgggaaact ttaccaggtg acacaagata tagagaatcc
    30541 ggatcaatat ttgttatttg gagaagaaac aatgaataag gttactcaat ttgtattact
    30601 tcaattgaaa gtaggtcaag aagctcaaaa gattgatgcg atcaagattt attatgtacg
    30661 gagtgcgtat taaaataaaa aatgaacact catatttatt atacaccgaa gaaatagaaa
    30721 aaaacagact gtttttttaa gaaaaactat gtcattcttt acttgaaaaa agttgttata
    30781 ggacagaaag gataaaacaa aaacggaaaa aatatgtctt atgaataact ataagaacta
    30841 ttctattttt taggagatgg atacgagatg aaaaaactaa ttggctatag tgtgatgtgt
    30901 attggatttg ttggtgtggg attgtttgta tcgactgaat ctgcttctgc ggctgaatgg
    30961 tataatccta tctggtataa tccgaacatt gaatgtcaaa aaggctatcc gtatggaccg
    31021 acaaatctga aaaatggaaa atgtagagta aattggaatg gcgttgtaga tagtgtaatt
    31081 actggttcaa tggactctgc tctacatggc tttacgggtc actagaagga ggaagaaaat
    31141 gtctgaagag aaaaaagaaa tcgttgaatc attagtagct ttaaaagatt ctctttctaa
    31201 gattgaatat gttgataggc aagaaattca aaaactaatt gatgatacta ttattgaaat
    31261 ccaagatgcg cgttgtgaag gtataaaaat ttctgtagct ttatctaaag taatagaaaa
    31321 aatgaaccgt tcattagcgt ttaatggatt gaagctagat agacaaacta gtctgatatg
    31381 ggatcattta aaagacttgt atgacaagtc taaaagaagt gaacggactg ctgtatcaat
    31441 cttaaaaggc ctttggggaa tgaattcata aacaagatat ttaaaaagta acttattttt
    31501 tatggaggag aaattatgaa aagaataatt aaaatatttg gtgttttagg tgttttctta
    31561 ttaggtttaa gtacttttgt tagtactgga acaagtgtgt atgccacaga aaaagtgaat
    31621 acagttgatt tagaaataaa gaataaatta gtagatgaat taacttttta ttttgataca
    31681 gttggttttt tagacgaaca tggtgtatac catataaagg attttggtgc tattcgtgaa
    31741 cgaatcagtc aagataacga acaaggaaga gcagcaagag tcttttatga acatattatt
    31801 gttcctagaa tggagaaagt tgattggaaa cgatatgctg gatgtgtagc cattaattct
    31861 cttcctggag gaggagtggc ttgggctata ggtacgcagg gagattttat tgaagcatta
    31921 aaagctttta attttgaaaa agctgcggat ttattattaa aaattgcaga aaaacaatta
    31981 ggaaaagctg cagctaaaga gttagctaag gtaaatattg tagcaggaat tgcagcagct
    32041 gctgctagct gtgcattaga gtaaaagttt atgaaaagtt tagaacaaga gcatatggct
    32101 tttaaacaag caatgtttaa agaaaatatc tatttaaacc ataactatat tcgtgtttct
    32161 aaggcatgtt caccagtatt gaatatgctt ggaggtggaa atggattata tcatttactg
    32221 tttgtcgatg tttgttggtt aattttttta ccagatgaac tagttatagt caatgaaaaa
    32281 ataacaagta aaaatgaagt gtttaattat tctttaacaa gaattaatta caaagagatt
    32341 accaagtttt cactagaaaa agttcctttt tggggagaat tttgtttaaa aattaaatgt
    32401 aactggaaaa gaatgtattt ctatattgat ggtgatgatg ctcttacatt cggtaaaact
    32461 actttttcta gttttaattt tcagtttcta ttaaaaaata atttttatgg tttattgaaa
    32521 taaggttgcg aaggaagatg tagagttatt tgcactgttg tgttggtata tctcttaatg
    32581 ctgtgtcttt ttggggagat taattagtgg agtgattata tgagtataga tgattgttta
    32641 gaagaacaaa taagattatt tttaaagttt ttaaaagtta aagttcaaaa taactacttc
    32701 acatgttata gttatgcaca ccctgtttta aatagtcttc cttttaattc tttagtgcaa
    32761 ggattgatta caaatattgt tttattagtc tgtacaccaa atgaaatatt tgtaaaaaag
    32821 ataggcagtg gtatttc
//
LOCUS       DAYYFD010000025        32694 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000025 DAYYFD010000000
VERSION     DAYYFD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.025"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            435..1160
                     /locus_tag="ACHA25_001061"
     CDS             435..1160
                     /locus_tag="ACHA25_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM8762371.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            1150..1824
                     /locus_tag="ACHA25_001062"
     CDS             1150..1824
                     /locus_tag="ACHA25_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM8762372.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(2112..2396)
                     /locus_tag="ACHA25_001063"
     CDS             complement(2112..2396)
                     /locus_tag="ACHA25_001063"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM8762373.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2690..3997)
                     /locus_tag="ACHA25_001064"
     CDS             complement(2690..3997)
                     /locus_tag="ACHA25_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8762374.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4486..5592
                     /locus_tag="ACHA25_001065"
     CDS             4486..5592
                     /locus_tag="ACHA25_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762375.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            6183..6383
                     /locus_tag="ACHA25_001066"
     CDS             6183..6383
                     /locus_tag="ACHA25_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8762376.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            6530..9319
                     /locus_tag="ACHA25_001067"
     CDS             6530..9319
                     /locus_tag="ACHA25_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8762377.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            9366..9893
                     /locus_tag="ACHA25_001068"
     CDS             9366..9893
                     /locus_tag="ACHA25_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8762378.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            9914..11104
                     /locus_tag="ACHA25_001069"
     CDS             9914..11104
                     /locus_tag="ACHA25_001069"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8762379.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            11144..12442
                     /gene="asnS"
                     /locus_tag="ACHA25_001070"
     CDS             11144..12442
                     /gene="asnS"
                     /locus_tag="ACHA25_001070"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8762380.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            13376..14050
                     /locus_tag="ACHA25_001071"
     CDS             13376..14050
                     /locus_tag="ACHA25_001071"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8762381.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            14047..14388
                     /locus_tag="ACHA25_001072"
     CDS             14047..14388
                     /locus_tag="ACHA25_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762382.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            14418..14777
                     /locus_tag="ACHA25_001073"
     CDS             14418..14777
                     /locus_tag="ACHA25_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8762383.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            15037..15159
                     /locus_tag="ACHA25_001074"
     CDS             15037..15159
                     /locus_tag="ACHA25_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762384.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            15301..17382
                     /locus_tag="ACHA25_001075"
     CDS             15301..17382
                     /locus_tag="ACHA25_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8762385.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            17693..19159
                     /locus_tag="ACHA25_001076"
     CDS             17693..19159
                     /locus_tag="ACHA25_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8762386.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            19444..19942
                     /locus_tag="ACHA25_001077"
                     /pseudo
     CDS             19444..19942
                     /locus_tag="ACHA25_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            20016..20456
                     /locus_tag="ACHA25_001078"
     CDS             20016..20456
                     /locus_tag="ACHA25_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8762387.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            20469..20678
                     /gene="copZ"
                     /locus_tag="ACHA25_001079"
     CDS             20469..20678
                     /gene="copZ"
                     /locus_tag="ACHA25_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8762388.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            20678..22864
                     /locus_tag="ACHA25_001080"
     CDS             20678..22864
                     /locus_tag="ACHA25_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8762389.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            22884..25055
                     /gene="copB"
                     /locus_tag="ACHA25_001081"
     CDS             22884..25055
                     /gene="copB"
                     /locus_tag="ACHA25_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8762390.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(25167..27338)
                     /locus_tag="ACHA25_001082"
                     /pseudo
     CDS             complement(25167..27338)
                     /locus_tag="ACHA25_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(27328..28080)
                     /locus_tag="ACHA25_001083"
     CDS             complement(27328..28080)
                     /locus_tag="ACHA25_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8762391.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(28077..28400)
                     /locus_tag="ACHA25_001084"
     CDS             complement(28077..28400)
                     /locus_tag="ACHA25_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762392.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            complement(28414..29289)
                     /locus_tag="ACHA25_001085"
     CDS             complement(28414..29289)
                     /locus_tag="ACHA25_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8762393.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            29616..30905
                     /locus_tag="ACHA25_001086"
     CDS             29616..30905
                     /locus_tag="ACHA25_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762394.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            30979..31548
                     /locus_tag="ACHA25_001087"
     CDS             30979..31548
                     /locus_tag="ACHA25_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8762395.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(31724..31900)
                     /locus_tag="ACHA25_001088"
                     /pseudo
     CDS             complement(31724..31900)
                     /locus_tag="ACHA25_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            31979..32677
                     /locus_tag="ACHA25_001089"
                     /pseudo
     CDS             31979..32677
                     /locus_tag="ACHA25_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT        10835 a         5711 c         6795 g         9353 t
ORIGIN      
        1 aatagtcaat cagttttttc acgacgtttc tattataacg aattatgttg aaaaaagctt
       61 ttatcgaaaa cataaatctt caaattaaca gattttttag acctcgatca tgatgaagac
      121 ttttgtagat acaaataact gtaacattga aaatgaatgc gaaaacgata ttttttttta
      181 ttggaaaata tcggtcaagc aataaaccag ttgcataata aaatgttaac ggcaacaaaa
      241 tctgtggaag gaatgaaccg gatgatttga tggaaaaaac aagatagaca agaagtatac
      301 atagtaaaac gtggaacaag taggccagaa tgaaaaatca gtttttatcc actcgctttt
      361 ctatacattc actgtcacaa atataatcga aagtaattct attttgagat aaagtatttt
      421 ataggagata gtatatgaac aaaaaaagat ttaaacgtat tgaaagaaaa tttgcattaa
      481 cttatgacca agctaagtat ttgaaaacac tatttccaaa acaggtagtt cgggataaat
      541 atagtgatca ttttatttct tccttgtatt ttgatacaaa cgatcatcac tttttaaaag
      601 aaatcactac agaaaagtcc tatcgagaaa aatttagaat cagaaaatat ggagatcctc
      661 aggacatatc gcctatctac ttagagataa aaagaaagtt tcaaggaatg acagataaac
      721 gtagaataaa attagcgcat catgagctcg atgatatgtt ggaaaatatc gaattggcag
      781 aaaccacatt cagtaattta gaagaagagt atatttttaa tgaaatacag tggttgttca
      841 gaaggttggc attggagccc aaagtatata tctattattt aagagaagca tttgtaaata
      901 gagattcaga agatttgcga atcacttttg atcaaaatat ccattaccgt ctaatgaact
      961 taacgacctg ggaacactgt acagaagaca atctggtagc gccagaaatc gatgtgctga
     1021 tggaagtaaa aacgacagga gaaatacctg aatggttaca ggtaggattg gataggatgg
     1081 gtaaaaaaga gataaaattt tctaaatata aacaagtcta taaaagatat atccaagagg
     1141 acccgctcta tgttcaatag tgtcttaaaa aatgagcctt ctcaactcac actagatgat
     1201 ttctttttat gtatggtggt gtcgattttg ctaggcattc tgattgctta cacccatatg
     1261 tatcaaaaca actgtacaaa aaacttcatg tttgccttga ttgggttacc tactttagta
     1321 cataccgtca ttttgttgat caacggtaac ttaggcacgg ggatcgctgt tctaggcgca
     1381 tttaacttag tgcgatttcg gtctgctcaa gggaattcaa gagatatttt cagtattttt
     1441 tgggcaacgt cgattggttt agccacaggg acgggatata ttgtctatgc actattcgtg
     1501 tctagtatag ttgctgctat ccaagtcatc atcttcacgc ttccaatcaa aaataaacat
     1561 acagttttta atactgaaaa aacgatcaag ctaaaagttt ctaaatcctt gtttctttcg
     1621 agtattattg aagaaaaaat tgcagaatat acagaaaact gcaatttaaa aaaagtaaaa
     1681 atcaataaag aaacttgttc cttacactat atagtaaaag taaaaacgag tatgcttgaa
     1741 aaagaattgg ttcgaacgat catggatcat ccagaagtca tttcgatcac tgtaggtatg
     1801 gataaatcga ataaattatt gtgataacca agaaagtact taacaatgaa gtaagacccc
     1861 agttccaaag tttagcgggg acacttcaat aaagtacttt cttttttgtc ttcagcaact
     1921 actggtaaaa ctgagagcaa gctttcgtga gagtacagcg ataaagatca acctcccttt
     1981 ttatctcgag caatttcctt gcaaggctgt ttcgttgatc ttaaacgagt tagcaacagt
     2041 caaagatgcg atggccataa aaaaattgca ttaagttggc tggtatctga tagctgggaa
     2101 tctaaggaag actatccatt ctgtttctta aattccgcta gttgatagat atcagccaat
     2161 tgttccagat gatcaacatc aaagataacc gaaagatgtt tccttttatt cccatttgct
     2221 tgaagagttt ttatcttctc tttggttcct tcactcaatg agagtgtgac taatttctcc
     2281 ctcgtttttt ctgggaaatg aaaggatact ttgaaaaatc cgtcccaaat agaaagccag
     2341 aagatcggtt ttactttctg agagtgggac aaaagtaaga aacacttttg tctcattctt
     2401 taaaaacgaa taaacggtgg gagcagaagc aactccttcg gaaataagcc aaaattcaca
     2461 aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc tcggagttaa acacttctgt
     2521 cccaacctct tgtattcacc ttttgatagc caagcttttc catcattata aaaccgcaaa
     2581 tttcaaaaaa aaacgaacaa tacaaaaagc gtattgctcg tttttctctt cagtttctca
     2641 cacctaaacg acttttgtct cgacctcttt ttggtgacta ttcatgatgt taaaataaat
     2701 tactgaaaaa atctgtgaca tggcgccaac caatgacaaa aatattagct ttttcaacag
     2761 tagaagtggt aatgatgtct gtcgaaggct cttggtttgc ttccaaatag cctaatttgt
     2821 catcagctaa ttggatcgtt gctgagccaa ctgtagtgcc tttgttgata ggagcggtca
     2881 cttggtcatc cgttactttc tttttatcaa ttgttggagt aatcgttaat tttccagtat
     2941 ccattccatt ttcgacccag acttttacag ggctttttag cgcaacattg acggaggttt
     3001 cttttccatc tttcactttg atgtctttta ggcttgggat gcttgcattt gcttttccaa
     3061 gttctttttc tgaccagttg tcataacaat aatccatcaa tcgtgatgtt tcattgaatc
     3121 gggcacttgg attttcttca tggttatctg catttaaaac gactgtgatg attcgtcggc
     3181 catctttgtt gatcgttccg acaaagcatg cacccgcaaa atcagttgtg ccagttttta
     3241 atccatcgac accttcgtga tagtttacaa aaccaggtag catccagttc caattgacca
     3301 tttcaaccgg tgactgtgtg ttttcgccga atgtttgatt tgtggtactc gtgattttta
     3361 agacttcagg ataatctaag atgaggtgac gagcaacaat agcgacatct ttcgcagaca
     3421 gttgattttc atctgtttca ccagtattct caggtcgatt ctcacctagg taagaattgt
     3481 ttaaacctga tgcattgacg atcgttgcat ctttgatacc ccaatctttt agttgtttct
     3541 tcattagctc aagaaattta ctttcactgc ctgcaacttt ttcagctaaa gcaaccatgg
     3601 aagcattggc tgattggatg aaagctgagt caacaagttc tttcactgta taatcatttt
     3661 ctttatgcaa aggaacattc gatagatccg gcacgacact taatttttct gcataatccg
     3721 aaatggctac cttatcttcc caagataatt taccctcttt gatttgttca agtacgatat
     3781 atagaccaat gatctttgtg atggaagcaa ttcccatcgt tttttcgcca tcctgatcat
     3841 aaaggatttt tccactttct gcatctacgg caaaggcagc tttggcattt attttaaatg
     3901 cgtcgtctgc ttgaacagtt gcgctaggca agaaagaagt gatacataag ataagacttg
     3961 ctaatagtac aataaaacgg gtattttttt tgaacatctt tgttctcctc atttttaaat
     4021 tgattcttac aacttagaat attatcagag agaacaaaag gtttcaatgg ttctgaaaaa
     4081 aaccttataa agaacaagtc ttattaatga agaatcatag aaagttatct tttgggttca
     4141 ttgacacgaa ctttgactgg ctgaacttcg ggttcggtta ggcgtgatgc tgcaaaaagg
     4201 gcgcctgctg agagtgcgac caaactaaaa atcatgaata attgcataag atcacctcgt
     4261 tcttttttgt ttttttatag tatagcaaga gagtttgaag ctactgttaa ggaaatgagc
     4321 atataattga tgaaagtatg aaaaaaatat gaagtaggac aacacaacta aaaatcgatc
     4381 aggtatttct ctggtaggga ttcgttttat tgtattgata aaatgggatg tggtagactt
     4441 tatttacaga tacaaagaaa tgattttttt aggaggggta cgtttatggc aaaaaaagaa
     4501 aaagaattat tatcacaatt gacttcagaa ggtaccaaag gaccgtttgt cacgattatg
     4561 ttaaacaccc atgttgcgca tcaagacgta gaaaaagatc aaatcaaatt caagaatttt
     4621 gcaaaggaag cgaaaaagcg ctttgaaaag aaatactctg agcatgattg gtctccattc
     4681 caagaaaaaa ttactagttt gttggacgat ccgtctttct ggcgtagtgc cacaacgagt
     4741 gtagctgtca tagtatcacc agaacaagtg tttatccacc gtttgggtat tcctgtagat
     4801 gaccagtatt atgtgagtga tctaccttat ttattagcga tcatcaaaaa tggacatttc
     4861 aactattcat actatttatt aggattgaat cgtgattcga tgaaattgta cgaagtaacg
     4921 aataaacaag tcaaagaagt ggctttgcct gaagatgcac caagagatgt cgtcactgct
     4981 ttaggatctg aactaacagg aggcgggttg aactacgcat caaatggtag tgaaggtacg
     5041 acgttccatg gagtcaatcc taaagatgaa gaagtcgaaa ttgattgggt caattattat
     5101 caagcagtcg atgactattt gaaagataca tttgaaaatc cagagaaatg gcctatcttc
     5161 ttatttgcat taccagaaaa tcagacgatg ttcaaaaaag tggcgaagaa cccttattat
     5221 cgttccgatg ttgcgatctc agcttcacct gcacaacttt cattaaaaga aattgaagca
     5281 ggcgcaaaaa aactatcgca agaattagct gtcaaagaag caagtgacta caataagttg
     5341 cttgataaaa agttcattga tcaactcgtt gatattatac cggcagcaaa agaagggaaa
     5401 atatcccatt tattcattgc tacatctaat ttagcagatg gttttggcga agatccagaa
     5461 acagaatatg accgccgtca ggtgctgaat gaactagcag ataatgtgat cacgaacggt
     5521 ggtcaggtct ttatccttga acaaaaagat gcaccagacg aaaagagttt agttgcgatc
     5581 ttacgttatt gagtgaaaac taccaaagta gatgaatggt caggcgttga tagaagtgta
     5641 tagctccgag agaaggagtt tcctctgttt cagccgttgg attcatcttg agcgagagac
     5701 aaaagctagc ttttgtctct cgctcattat ttttgtgctt tagtcccatt ggctttgcga
     5761 ggggaagata cttttctcaa aaaaacgaac cttgcagttg agagggggat agatgcgcat
     5821 actaattttt atcagttaaa aaatgatgtg gatcattaga gaattccaaa gttcgttcat
     5881 cctttttcag ataataaatt gaaatttttt gcaaaagtga aaatagcaaa tagaaatttt
     5941 ctgaatctta taggggtgat ttcgagaaaa cgttcgttta tttgcaaata tatcttctaa
     6001 ataagagaat aaatacaaaa aaataaggaa aaactctggc aattttgtaa acggtatgat
     6061 atagtaagaa tgtagttttt gttcggtaat ctgtggtagc ttgtttcaaa taaacatctt
     6121 ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg aggattttta
     6181 ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga ttcatcactg
     6241 cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac ggattcaaaa
     6301 ctttagaaga aggccaagca gtaactttcg acgttgaaga aggtcaacgc ggacctcaag
     6361 caacaaacgt tactaaagct taatttacct atattaaaaa taaaagtcca gacatttatg
     6421 tgtctgggct tttttgtctt tttttcctta aaaagcgatg agagtatttt tctgcacttt
     6481 gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt tgaaaccaag
     6541 aacttatgca gtcgttgata tcgagacgac tgggacgaat ccaaaagaag atcggatcat
     6601 ccaatttggc tgcgtcatga ttgaagctgg tcggatcact tcacgttttt cgatcgatat
     6661 tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca cgaatcaacg
     6721 cgtccaaaaa gcgccttatt ttgaagatgt cgcccaaacg atttacaatc ttttggcaga
     6781 caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc atgaactcat
     6841 gcgatgcgga cttcctagtt tgaagatacc gggcatcgat accgtagaac ttgcacaaat
     6901 tttcttgccg acggaaccaa gttttcgctt agctgatttg tcagaaagcc ttggctttat
     6961 tcatgagaat cctcatcagg cggatagtga tgccgaagtg acaggtcagc ttcttttact
     7021 gatcgaagaa cgaatgagga aattgccgat catcacatta gagcagattg cacgattaag
     7081 tagccataca ggaatggata cgagtcgttt catttaccac gtgcttgaag aaatgaaaga
     7141 aaacccggag ccccttgatc tctcattaga aatcatcgat gggctagctt tgcagaaaaa
     7201 agaagtggag ctattcacat ccgtccatta tggcgagcgg acatatccaa gaaaaaaaca
     7261 agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga acaggctgat
     7321 gaatgctgtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga tcgaagcagc
     7381 tacaggaatg ggcaaaacga ttggttatct gttgcctttg agttatttgg ctacacctga
     7441 aaaaccagct gtcatcagca ccgtttcgct tgttttgcag cagcagatca tggaaaaaga
     7501 cattccatta ctgaatcaat tgctagatca gccgatacaa cctgtgatta tcaaaagcta
     7561 tcgccactat attgatttac agcgttttaa aggtacactt gtggaaccgc cgggacaaaa
     7621 acaatatgct ttgtaccaaa tggctgtctt agtttggcta acacaaacga agaccggtga
     7681 tttggatgaa ttacatttaa ccaatctgaa ccatgcattc tttgcagatg tcgcgcacag
     7741 aggcactggg tttttagcaa ggaatcagtc attttacaaa caagactttg tgcgtttctt
     7801 gcaaaagaaa atcaaacaaa gcaatttttt gatagtcaac catgcatttt tagtacaaga
     7861 aacacagcga aaagaaccat tgcttccagc tagtccttat ttgttgattg atgaagtgca
     7921 tcatttgccg gaaattgctg aaaaagtaaa tgacaaacga ctaagtatta actacttcca
     7981 taaacaaatt cagcaactgg aagagccgaa tcagttattt gaccgcatac aaagttattt
     8041 gccgaaggaa catgaagcct tgcgctggtt gtcgatttat cgagaagaac tctctgcact
     8101 atgcgaagcg cagatttgcg tggcagaggg attaatgaac ttagtaagca gcaagcaacc
     8161 taacggacaa tatccagaag aagtcatgat gacaaaagaa atgttggatc agctgccact
     8221 ggaatctgga acagcaatgg atcatctgca acttttttat caagagatcc aacaactaca
     8281 atcacagatg cgcttgactt ttgagattat ccaagaaagt tggaccaacc agatgcggat
     8341 cgatttagca agtttatata gcttatttga tcaattagac aagcaatact tgctgatgga
     8401 ccaatggcta gaagactggc gagatcattt gatccattgg ttcgttcccg ggaatacagc
     8461 acagcagatg atttttcatc tccttgattt tcaagcagct atgttgccgt caactgtgtg
     8521 gtatcctcga tatgaccata ttctttatat tggaggaaca ctgaagatcg gacctaatcg
     8581 acagtatctc tctaaacgac ttggcattga agcagcacct cttaaagtga ttgcttctcc
     8641 ttatgattat gagaaacagg caagaatcta tgtgccgaaa gaagcacctg cagtccatca
     8701 agtcagcaat caagcatata tcgattattt agaagcagca ctcacaggat tgattgaaca
     8761 agaagatcgg ccgatccttg ttttattcac ttctcatgat gttttgcaaa aggtatatca
     8821 gaaaatgcat ttgtctatgc tagaaaaagg acgtgaaatt cttgctcaag gatttggcgg
     8881 gagtcgggaa aaacttttga aacgcttcat tttgtcagaa aaaagcgtct tatttggtgc
     8941 tgacagcttt tgggaaggcg tagatttacc tggagatgct ttgcagattg ttgtagtcac
     9001 acgtttaccc ttcgacaacc cgttacgtcc gatggtcaaa gctaggaatg cttatctgga
     9061 atcagagggg atcaatcctt tttatcagga atccgtgcct aaagcagctt tgaagttacg
     9121 ccaagcatta ggtcgtttga taaggacgga gacagatcgt ggcgtattac ttattttgga
     9181 tcggagatta gtcactgcaa aatatggtgc acgaatcatc catgcattac cgaaaaaagt
     9241 accgataaaa gagctagcta tagatgaaat ctatcaggat atccatgaat tcttaaagaa
     9301 aaatgaagaa ggaagttaaa ggtgccgtct tttttcatca ttaaaatctg ctataatgaa
     9361 aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag gaaaaaacga
     9421 ttaatcgcgt gttgcttggc ttgatcgtat ttttattagc ggtcatcgtg atcagctcga
     9481 ttttttactt gcgttccagt cgtccggcgg tccaggcgaa aaaagaagca gtagaaatcg
     9541 caaagaaata tgcaaatctc gaggaagccg atcattttta ctggtttact cgaaagaaaa
     9601 cgtacttttc attgacgggt acgaatgata aaggaaaaga aatcgccgtt atcattccta
     9661 aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa aacgaagcaa
     9721 aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct ttagggatgt
     9781 atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa ctgaattatt
     9841 acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt taaaaggtaa
     9901 aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt ctgtcacttt
     9961 ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc tgagtttgac
    10021 tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa tcgaagcaat
    10081 ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc ttcgacaggc
    10141 aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac aaacagtcgt
    10201 aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg acccgcaaga
    10261 tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa aactggcaga
    10321 aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta ctgttgagca
    10381 gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc catcgaatcc
    10441 gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg cagtaaaaaa
    10501 tgatattttg atagttgctg atgatattta tggccgttta gtttataatg gcaatgagtt
    10561 tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca tcaatggcgt
    10621 gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggga attctgagat
    10681 catcaatgga atgattgctg ttgcttccca gtcaaccagt aatccgacag cagtcagtca
    10741 atatgcagct gttgaagcat tgaaaggcga gcaagataca gtggaagaaa tgcggaacgc
    10801 ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccgg gtgtttctct
    10861 tgaaaagcca caaggagctt tttatctttt tcctaatgta aaagaaacgc tacgcatgtg
    10921 taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag gggtagcgct
    10981 tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg ccactgatcg
    11041 cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta aaagtcaaaa
    11101 ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac aaattcaaat
    11161 catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg tcgcaaataa
    11221 gcgatcaagc ggaaaaatcg catttttgca attaagagac ggaacagcct ttttccaagg
    11281 tgtagttgta aaaagcgaag tgccggaaga agttttccaa acagctaaaa atctacacca
    11341 agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa aatttggtta
    11401 tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc cgatcacacc
    11461 aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc gttcttcaag
    11521 acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg aattctttaa
    11581 tcaacatcat tttgtcaaaa tcgatccgcc gatcttgact ggttctgcac cagaaggcac
    11641 tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc aaagtggcca
    11701 attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg gtccgacttt
    11761 ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga tcgaaccaga
    11821 aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg tagcattctt
    11881 agtacaaaat gttttagatc attgcgaata cgcattagat gtactaggac gagacaaaga
    11941 agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttatgatg atgcgatcga
    12001 attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg gttcacctca
    12061 tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact atccaaaagc
    12121 catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtga tctgtgcaga
    12181 tatgatcgca ccagaaggtt acggtgaaat tattggtgga agcgaacggg caacagacta
    12241 tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat attcatggta
    12301 tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag gccttgaacg
    12361 aacagtcact tggattgctg gtatcgacca tgtgcgtgaa gctattccat tcccacgttt
    12421 gttgaaccga atttatccat aagaggttgt gaaatcagtg ccttgacctg agtattagag
    12481 aaacaaaacg gaaaaatagc ttctcatatt ttactgtttt ttgagctaat atcgagggtc
    12541 agctgattga acaccgttta tatcaagagg ttgtgcgtct gccgttcagc tccgagtcac
    12601 aaggagtaat ttcaaaaaaa cattttttca tgtttgatga ctattgtgac ttgtgaccaa
    12661 ggagttggcc ggagagcaac gtttatttaa aaaaaagaac tgcgaactct cgcagttctt
    12721 tttttttaaa tacgggataa aggaagtttc cagcagataa gagaagaaag caaaagaaat
    12781 tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat atacaaagaa
    12841 ggttattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa agcagcatct
    12901 agcacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt attttcagca
    12961 tgcaatgatc catatgtaag ttcaattcac ccttgaaatc atcggaccag ataaagttat
    13021 ggccaatcta tcccaagcaa tctagaagag atattagtaa aggaacattt actggaagtc
    13081 ttagatgagc ctataaatat atgttctgat ggcgtcatca agtggtctga ttgacggaag
    13141 cttaggtgtg atcgatctct ctggaccaaa caatgaagga atcaagggga atccagcacg
    13201 ttaagaaaaa aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa
    13261 gggactaggc tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta
    13321 tgggataata taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa
    13381 aaaacgtata ttatttatcg gtgacagcat tacagacgca ggaagagacc gagcagaagc
    13441 aactgatcta ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc
    13501 aacgatggaa tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca
    13561 agtacgctgg cgagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg
    13621 aatcaatgat acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc
    13681 tcagcaattt gaatccgttt atcgaaaatt actaaatgaa cttcaaaaag cgggaatctg
    13741 caacatcatc ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg
    13801 taaagactta gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt
    13861 gctggttcct ttagatggca tattcaatgc tttagggatt gcttatggtt tttcgaaata
    13921 tacaagagaa gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg
    13981 gatagatgca attgccgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag
    14041 tgagaaatga tggaaatacg gtgggcagta aaagaagatc tacctcaaat caatcagctg
    14101 attactgatc atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag
    14161 aataaactat tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggttt tgcgcaagcg
    14221 gacgtgcaac agtcagaaga acgggtgggt cagcgaccat caggaaaact aatccaacac
    14281 acattagtaa caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atatttgatg
    14341 ctggaagcta aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta
    14401 atagaaggag aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac
    14461 taatcggtgg tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta
    14521 tcccaatcgg tatgcaatgt tttaagatcg ccttcctttc ttttgcgcct tttggcaaag
    14581 aagtaattta tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt
    14641 caggcctacc aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca
    14701 tagggatacc ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg
    14761 gtgcagaaat tgtataaaag agaataataa aggttagata aagcatagag tcaggtggga
    14821 tacatgtatt tcacctgact ctatgcgttt tcctgtataa gttcttccat gtgactttta
    14881 cgtcttttat ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa
    14941 ctctttctat atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca
    15001 gggattgaaa aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca
    15061 acaggcttca aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata
    15121 agagcagtgg caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag
    15181 aaattttccg atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac
    15241 cattcattta taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa
    15301 atgaaaagtt acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa
    15361 ggagttcaga aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta
    15421 acgattgacg cggaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg
    15481 aaggaattag aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat
    15541 ggaaatgaaa aagagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct
    15601 ttattgctat tatttgattt taatgaacta attcgcttga tagggtattt attgatttat
    15661 ctgctgatag gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc
    15721 tttgatgaga attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat
    15781 ccagaagccg tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca
    15841 gtcagtcatt caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac
    15901 gtgcagacgg cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt
    15961 gtacttgtga agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca
    16021 ttagtcgata cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa
    16081 acagtattaa gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct
    16141 tatgaaaatt ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa
    16201 gcaccagctg aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga
    16261 ttggccgttt tattagctgt tttacctcca cttgttgttt ctggagcggc gttttctgaa
    16321 tggatctatc gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc
    16381 gtgccactta gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa
    16441 ggttcaaatt acttggagtt gcttgcgcaa accgagacag tggtattcga taaaaccggc
    16501 acattaacga aaggcgactt ttcgatccag acgatcgata caaaaatcga tccaaagata
    16561 ttcatgcaat atattgcgag tgcagaacag ttttctacac atccgattgc tcaatctgtt
    16621 ttagaaggct acgcaggtcc gcttttacca acagctaata tccaggaatt tgcaggtgaa
    16681 gggatcttag ctgaagttga tggcaaacaa gtattagtag gtaatcacaa attgatggaa
    16741 agatttgaaa tctcttttcc ttcaagtcaa gaaatcggta cattgcttta tctagcaatc
    16801 gatcaaagtt acagtggtta tcttgttatt gcagatacct tgaaagaaga tgccgtagac
    16861 gcattggttc aattaaaaca ggctggtgtg aaaaacacag tcatgttgac gggcgactcg
    16921 aaaaaaattg ctgatcatat cgggaagcaa gtaggcgtgg acaagatata tagcgaattg
    16981 cttccagaag ataaagtgca gcggttagag gaaatcttac aaagtaacaa taaaaagacc
    17041 gcatttgtcg gagatggaat caacgatgca ccggtgctag caagagcaga tgtaggaatc
    17101 gcaatgggtg gattgggaag cgatgctgca attgaagcag cagatgtagt catcatgaat
    17161 gatcaaccat cgaagattgc agaagcaatc catttagcaa agaaaacatt gaaaatcgtc
    17221 aaacaaaaca ttgtctttgc tattgggata aaaattttgg tactttcctt aggtgctttt
    17281 ggttttgcat caatgggaga tgcagtattt gcagatgttg gggtgacagt attagctgtt
    17341 ttgaatgcca tgcgaagctt gcatgtaaaa aataaacgtt gatcaatgaa gaggacgaat
    17401 gaacgtcctc ttttttttat atccttttct taatgaaaaa tgtaaagaaa atagatagga
    17461 ttataaaata actaatgttt ttaattttgc agaaaattca aaatagcttg aaattcaata
    17521 aggtcaatga aataaaagaa aataacttat ttttttgatg gaaaaaatat gaaaaaacga
    17581 tgtgtattaa ataaaataat acacacataa aataacactg tcttgtaagt ttgttaaaaa
    17641 ttgaatatga ttacatctgg ataacaaatt tttatcataa aggagagaac gaatgaacaa
    17701 caaacaatta cgctggttta ctgttgcgtt aattgcattt aacatggttt ggggcatggg
    17761 gaatgtagtt aataactatg ctcagcaagg agtatcagtt gtcacctctt ggctattgat
    17821 tttggccatt tattttattc catacgcatt gatcgttgga caattaggat cagccttcaa
    17881 ggatagtaaa ggaggcgtca gttcatgggt cgaaaatacg acatctaata aacgactggc
    17941 atactatgcc gcttggactt actgggtcgt ccatattcct tatttggcac aaaagcctca
    18001 agcaattctg attgctggtg gttgggcagt ccaaggaaat ggaaatatcg taaatacgat
    18061 gtcagtacaa atggtagcag ttatttcact tattatattt cttgcctttt tatatttatc
    18121 aacaaaagga ctttctactt tgaaagtgat tggcggactg gccggaacag cgatgttcgt
    18181 catgtcgatc ttatttattc ttttagctgt aacggcaccg ttcatcaatc caacgatgga
    18241 atttgccaca ccacatatga atcaagtgaa aacatatatt ccaacatttg atttcagtta
    18301 tttcacaacc gtttcgatgc tagtatttgc agttggaggg gcggaaaaaa tatcccctta
    18361 tgtcaactct acaagaaatc ctgcaaagga atttcctaaa gggatgatct tcttagcagc
    18421 aatggtaggg atttgtgctg tattaggctc agtcgcaatg ggtatgctgt ttgctagcga
    18481 taatatccct aaagacttga tggcaaatgg ggcatacagt gcttttcaaa ttttaggtaa
    18541 ttactatggt atcggtaact cattgatgat catttacgca ttgacaaaca tgattggaca
    18601 aatctccgca ttagcctttt caattgatgc accactaaaa atcttgttag cagatgcgga
    18661 tccagaattt gtaccaaact ggctacgtaa acgtaatcgt aaaggtacat tggtgaatgg
    18721 ttatcttctg acaggtatac tagtaggaat cattattttg ttgccgatct ttggtatcaa
    18781 agatatgaat gaattagtaa aatggttgac gaacttgaac tcagttgtca tgccaatgcg
    18841 ttacctatgg gtgttcttgg catatatgat gttgaataaa gcttataaga aattcaattc
    18901 tgaatataaa tttgttaaaa atccaaaaat cggttttatc tttggtgctt ggtgctttgc
    18961 cttcactgcg tttgcatgta tcctaggaat ggtgccaaaa gtagattata ctgctgatcc
    19021 taaagcatgg tggttccaat tattcacgaa tatcatcaca ccaattgtct tgatcttatt
    19081 aggtatgatc ttgccagcaa ttgctcgtcg agacaaaaac aaagaaatta ctgaaccaga
    19141 aacagtggtt cagccttaaa atagaaatac ttgttacaag aggttgtgac agaagctact
    19201 tctggaacac cgtgaatcag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    19261 gccaaacaaa aatagctcct catattttgc cgttttttga gctaatgctg aaggtcagct
    19321 gattgaacac catttattcg aaacaaggga ctttgacacg acttttgtca cagtcccttg
    19381 tttttttgcg gcgttataag tggtatgatc gaaccaaatt ccaaagagaa gtgagggaga
    19441 agaatgaaga tcatcgtgat tggacaaagt gggtcgggaa aatcgtccct cgcaaggaag
    19501 atcaaagaaa tcaccggctt tccgcattca ggcaaaaaag tggtttttgc aagaacagca
    19561 atcgtttata gtatctaatc cgtcatggat cgttgaagga aattatactt ctacgttaga
    19621 ggaaaggatt aaagaagcag ataagatcat ttggcttaaa gtgccgcgtt atctcgccat
    19681 ctaccgagta gtttggcgtt ctctaaaaag aaagataaac aaaaaatctc ggccagatat
    19741 gcctgaatca ttttcggaga aactaaacag gaaatactta gaatttttat cttttatctg
    19801 gcattttgaa ggaaacaatg aaccaaaaat acagcagctg atccaagtag ctaatgcaag
    19861 aaataaactg atcatcctta aaacaagacg agagaaacaa gaatttttaa ctaaattgaa
    19921 aatagagagc aaaaataatt gacagttttt gtttacaagc gtaatctgta ttcataagct
    19981 aatgattaca tttgtaaacg aaaggtgtga aataaatgga aaaagtcaaa acgccagtaa
    20041 agatcagtga ttcagagtgg gagatcatgc gtgttatttg gacacttgga cagacgacag
    20101 cccaagaaat cacgcagatc ttagctgatt cgatggattg gaaaccagca acgatcaaaa
    20161 ctttgctggg acgtttggta aaaaaagaag tgatctggac agaacaaaaa ggaaaaaaat
    20221 tcatttatca tccagctgtc tcggagatgg aaacagttcg cagtgcgacg gaaaatcttt
    20281 tttcccatat ctgtgcaaaa aggattggag agacgattgc ggatctagta gaagaagcta
    20341 ctttgactca agaagatatc aatgagattt tgaaacaact ggaacaaaag caaccagtag
    20401 atacgatcga atgtaactgt atcccaggtc aatgtgaatg taaacaacat caataggagg
    20461 aagaaaaaat gaaacaacaa ttttcaatcg aaggtatgag ctgcaatcat tgtgcagcaa
    20521 gagtagaaga aacagtaagc gcacttgacg gcgtgcaaaa agtgaaagta aatttgaaaa
    20581 aagcaaacgg tacggtcaag tttgatgaaa caaaagttca atccgaaaag atttgtcaag
    20641 caatcaatgg attgggttat aaagcagagg tgatttgatg gcaacagcag aaaccaaaat
    20701 ggatacgttc gtcattacag gtatgacttg tgcaaattgt tctgctagga tcgaaaaaga
    20761 gctgaacgga caacctggtg tggtcaatgc cacagtcaac ttggccactg aaaaagcaag
    20821 tgtcaaatat gaagggacga ccactgaaaa attaatccaa agcgtagaaa acatcggtta
    20881 tggtgcaatt ttgtacgatg aagcccacaa acaaaaaatt gcagaagaaa agcaagcgta
    20941 tttgaaaaaa atgctattcg atcttatatt gagtacggtt ttgactttgc cgttgatgtt
    21001 gtcaatgatt gctatgatgt taggaagcca tgcggcgatt gttcactttt ttcattttcc
    21061 aatcgtccag ttagtgctgt ctgctcctgt ccaattttat gtcggtgctc gtttttataa
    21121 aggtgcgtac catgcaatca agacgaaagc accaaatatg gatgtattgg tagcaattgg
    21181 aacatcggct gcctttgcat tgagcattta caacggattt ttccgtggac acccccaaga
    21241 tctttatttt gaaagcagca gtatgatcat cacgctgatt ttgctcggca aatatttgga
    21301 acatacagca aaaacgaaaa caggtaatgc aatcaaacaa ttgatgtctc ttcaaacaaa
    21361 aaccgcccaa gtgataagaa acgggaaaga agaaacgtta gcgattgaag aagtagtcgt
    21421 tggtgatcaa ctagtcatcc gtcctggtga gcagattcca gcagatggtc gaattatttc
    21481 aggaagcagt gcgatagatg aaagtatgct tactggtgaa aatcttcctg tagaaaagaa
    21541 tccggatgac acgctttttg gcgggacaat caatacgaat ggtctgctac acatggaagt
    21601 cacacaagtc ggcaagcaga cagtattagc gcaaatcatt caaatggtgg aagatgctca
    21661 agggagcaaa gccccaatcc aaaaaatcgc agatcggatc tcagggattt ttgttccgat
    21721 cgtattagtc atcgcgttta tcacgctcat agctacaggt ttgatcactg gtgattggca
    21781 actcgcattg atccatagtg tctctgtatt agtcattgcg tgtccatgtg cattaggttt
    21841 agcgacccca acagctatta tggtaggaac gggtgttggt gcacgaaatg gaattttaat
    21901 taaaggcgga gaagcattag aagcagctgc tcacttagac agcattgttt tggataaaac
    21961 tggaacgatt acagaaggaa agcccaaagt tacagatcta gtcggttcaa aagaagtcct
    22021 atctattttt tatactttag aacaagcttc tgaacatcca ctaggtaaag caatcgtgga
    22081 atacggaaaa ctgcaagaag cagcaacata cgatatgatt gattttacag cccacccagg
    22141 tgctgggatc agcggtacga ttaatggagt acgctatttt gcgggtacac gtaaacgatt
    22201 aattgaattg aacctatcat ttgatgaata ccaagaacac gcgttagaat tagaacagca
    22261 gggaaaaaca gtcatgtttt tagctgatga aaagcaagtg atcgggtgga ttgccgtggc
    22321 tgatcaaatc aaactagaag ccaaacaagc aatcaagcag ctccagaata aaggattaga
    22381 tgtttttatg ctgacaggag acaacaaact ggcagccgaa acgattggta aacaagttgg
    22441 tattgatcca aagcatatct ttgctgaagt acttccagag gataaagcag cctacgtaga
    22501 aaaacttcag aaagatggca aaaaagtcgg tatggcaggc gatggaatca atgatgcacc
    22561 ggccttagca ttagctgatg tcggtatggc aatgggaagt ggaacggaca ttgcgatgga
    22621 aacagccgat gtgacgctga tgaatagcag tttggcatcc attgcacaaa caatcgagtt
    22681 gtctaggttg acattgagaa aaatcaaaca aaatcttttc tgggcttttg tctataatac
    22741 gattggtatt ccttttgccg cactaggctt tttgaatcca atcattgcag gaggagcgat
    22801 ggcgtttagt tccgtcagtg tattgttgaa ctccctgagc ttaaatcgac acatgaaaaa
    22861 gtaagagaag ggatggagta aaaatgaatg ggaacgatat ggagaaaaaa catgagcata
    22921 aaaaagaagt agtaaacaaa gaaagtacag aatctcatga aaaacagatg gagatgaaac
    22981 atgaccatag tcagatggat cattccatgc acatgggaca taaccatgga gatatagcaa
    23041 acagcaaaca aatggaccat tcgatgcaca tggatcacga acacggcggc atggatcatt
    23101 ccatgcacat ggggaatttc aaacaaaaat tttggctatc gcttatccta gctatcccaa
    23161 tcattctttt ttcaccaatg atgggaatgg aatttccatt tcaagtaacg tttcctggtt
    23221 ctgattggct cgtattgatc ttagcaacta tactatttat ctatggcggt cagccatttt
    23281 taagtggagc taagatggaa ttgaaacaaa aaagtcctgc tatgatgaca ctgatcgcga
    23341 tggggattac agttgcatat atttatagtg tctattcgtt tatcgcaaat ctgctcaatc
    23401 cacatacaca cgtcatggat ttcttctggg aattggctac tttgatcgtt attatgctac
    23461 taggtcactg gattgaaatg aatgctgtgt cgaatgccag caatgcttta caaaaactag
    23521 cagaattact gccagaatca gtgaaacgct tgacgaaaga tggaaaagaa gagacagttt
    23581 cattgaaaga agtaaacgaa ggtgatcgtt tgatcgtccg gtctggtgat aaaatgccaa
    23641 cagatggtgt aatcttgaaa ggtgaaacaa ttgttgacga atcagctgtg actggcgaat
    23701 caagaggaat caaaaaacaa gccaatgaca aagtgattgg cggttctatc aatggggatg
    23761 gaacgatcga aatcgaagta actggaactg gtgaaaatgg ttatctagcg aaggttatgg
    23821 aaatggtgcg aaaagcacaa ggagaaaaat ccaagctaga gtcattatct gacaaagtgg
    23881 caaaatggct gttttatgtc gcattaattg tgggtatcct ggcattcatc gcctggttgt
    23941 tcttgacaga tcttcctaat gcattagaac gtatggtaac agtctttatc atcgcctgtc
    24001 ctcatgctct aggtttagct atcccattag ttgttgctcg ctccacatca atagcagcta
    24061 aaaacggatt acttttgaaa aaccgcaatg cactggagca agccaatgat ctagatgtga
    24121 tcatgctgga caaaacagga acactaacag aaggaaaatt tactgtaacg ggtgtcgaag
    24181 tacttgatga tgcgttcaat aaaaatgaaa tccttcaata tctaggagcc ttggaagcga
    24241 atgccaatca tccattggct gttggcatta tgaattattt gaaagaacat gaaatcaagc
    24301 cttatcaagc tgaaaatcta aaaaatcttt caggagtcgg gctagaggca acagtcaaaa
    24361 aacaacaagt gaaaatagtt aatgaaaaag aagttgagcg tctcggatta aacgtagaac
    24421 aagcattatt gaaaccttac caagaacaag gaaataccat cagcttcctg attttagaaa
    24481 atcatctagc tgcaatcgtt gctttaggtg acgtagtaaa aacagaagca aaagaattta
    24541 tccgaacatt gaaagaaaga aaaatcactc ctgtcatgct tactggtgac aataaaaacg
    24601 ctgcccaagc agtagcagat tatttaggga tcgaagaata ttatggcggt ttattaccag
    24661 atgataaaga agctgttgtc cagaaatacc ttgaccaagg gaaaaaagta atcatggtgg
    24721 gtgatgggat caatgatgcg ccaagtttag cccgtgcttc aatcgggatg gcaatcggtg
    24781 cagggacaga tatcgcaatc gattctgcgg atgtcgtatt gacaaacagc gatccaaaag
    24841 atattttgca ttttcttgat ctagccaagc aaacgcgaaa gaaaatgatc cagaacctct
    24901 ggtggggggc aggttacaat attcttgcaa ttccgctagc agccggaatt cttgcgccaa
    24961 ttggtatcat attaagtcca gcagttggtg ctgtcttgat gtcgttaagt acggtagttg
    25021 tagctctaaa tgctttgaca ttgaaaatca aataataatg aaaaaaggtt gtgacaaaaa
    25081 acgtgtcaca accttttttt cgagtaatcg atactcaatc agctggttct cggcattcca
    25141 gaagtagatt ctccagaaat tttatcttat tttttgaagc ggctcccttt tgtcagaatt
    25201 tttttgtata ccgtttttct tattatacgc gttatttcta aataggtttt tttatcgaat
    25261 tttaccggat ctgcttgtcg tccatatatt tctgcttcat attgagtagt taatcgaatg
    25321 aaacgcagat ctagcttgaa ttcttgttct attcgcaggc tatattcctg caaagattct
    25381 gcgggtttgc gcggtaccaa tgtttccaaa gggttaagaa gtttttgata tgcaatcgta
    25441 ttagtagaag caaaaaaatg aagatagata agataacttg tccagcgcag agattttctg
    25501 aataacaata gaaagaacaa caagacggat aaacacactc caaggctgaa taagacgatt
    25561 ttataagctg gtgtgagtgt tgtttcttct gatggaatga cctcagatct ctcactggaa
    25621 atagtttctg ccatattttc gctttcattt ggttgttcga tagcaggctg gacggtttcg
    25681 gttgtttcag caactggttg tgaacccatt ggattggcaa atgaaggggt tggttcaaat
    25741 ggaagccagc caaatccagg aaagtatacc tctggccaag aatgagcatg actattgaga
    25801 atgctgtatt cctcattccc ttgattatct gtgcctgttc tgagcccatt tgtaaaacct
    25861 tttgtccagc gagctgggat accaagtgtt cgaagaagga cgaccataga agttgaaaag
    25921 ttgtcacaat agccgacttt tgaatcaaag aggaaataat ccacataatc gtgttctttc
    25981 ggagtatgcc cggtatccgt tttggaatag cggaaatttc cgcttgtttt taaatactgt
    26041 tagacagctt cgactttacg aattaaagtt gcttcatttt tggtcaattc gaccgccaag
    26101 tcctcaatac gtttaggcaa actttttggc aattgaagat ttgtctgctg attttctaaa
    26161 tcatcagctt ttaactgact gctttttaat tcttctctcg taaaaactaa cctttctgtt
    26221 tgtaaagttg cagaacgtgt cgattcattc gggacaggaa tgaatcgctg gctttcaggc
    26281 aaatagtaaa gcgtttgtcc actagctaat gaaaaatcag tcaaggtagt atttccataa
    26341 ggataaggta agaaagcttg tttttttagg gtcaatgtaa tgttttcttt gtcttctata
    26401 acggtttggt agctcgtgat gtcaacaggg aattcttgga ttggttggat gggtgcgtcc
    26461 tgatcttcta tccatccaga accggtgtaa tgatttttat tttcgatctt ccaatagcca
    26521 tttccttttt ggttcgctgt aaaaacaact tcgccattat catataaagg tccaccaagc
    26581 tgttcatcat tctcactgaa gcctgttctt gcacttccgg ttaattgata ctcatggagt
    26641 gtgtcataaa gcccttcatc attaagatat tctcggatcg gttcactcgt ttcctcgaga
    26701 gattggtgta ctttcggaag atataaaggc aagatagcgc taaaagttaa gaaacaaata
    26761 agcagaactg tagtcaatga ctggaatcga gtgtttcccg acaacaaccg gatggagaaa
    26821 aattgccttg ctgccagata cacgaacaaa acagttacta ccgcaatcat ttcgtacagt
    26881 aaatcttttt tccgaaaaac ggtaagcatc atgaagtaag ccatcaaaga aagaaagggt
    26941 gcgtaccaat agtcgtaatc cacggtcaat gtaataaata aatagatcag caacattatg
    27001 agaaaaaaag ctgtcttttc tggcaaataa taaagtttac cttgggatag atggaagaat
    27061 tcctctgtaa ctgacgaaaa aaactgtaag atccataata tggaaaatgt cttcgaagga
    27121 aaatataaaa acaaacaacc tatcattact ggcaaagaaa gcagcaaacg tacccatttt
    27181 ttaggaatac agcagacaat aaggcatagg acaccaatag ttaggataag aaggtagctg
    27241 ttttgcaatt gattagctac tacaaatatc ggagcaccta cacttagtat cacaaaggag
    27301 taaacgccac gcagccattt ttcccgttta atcgtcaatc cattcacctc ctggcaatga
    27361 acatatcttg tctttttctt ggaccatgag ttgtttttct acgaaataaa caagataagc
    27421 cgttcgatct tttgacaatg tttcaatagc ttctgagact tcaggagaat tctcaggaac
    27481 aaagaggacg atttgttttt ttgcatattt tttcaagtgt gcgaaaaaag ctttctcatc
    27541 gccagcttcc aaagaagcaa aataggtatg accggggtct ttgccatgaa aggcgcggtt
    27601 gcctaatatc agaagttccg gctgcttttc ttttctcaaa tgccataaag aaaaatacaa
    27661 gtctagcaga ggttcaaatt ttggactggg cgaaccccaa aagcaaaaaa gaggagaaca
    27721 ttcttcttct tgataatcgt attcacggta taaccactct tgagtatgag cagacaattt
    27781 ccaatcgatt cggttgaaag aatcacctgg acgataagcg cgatgttccc gaaaatcaag
    27841 acctctttgg tacgcgttca gctgctgttt tttttccaga actctctgta atttttctgc
    27901 ttgaatctta caaaaagcag gcagaacagt cacaggaaaa gagatctctc tttttgcact
    27961 ttttctgaaa agtcctagac tatcataggc tgtaaaaatg acagttaatg tttcatggca
    28021 tcctctttct tttggctgcc aaagcaattg gacttcttgc ggtttatttg taagaatcaa
    28081 aatctcttgt ttttttctgc aaagattgct gggaagccga tttgtaaacg agggagaggt
    28141 aaagaaaaag ttctcttttt tgctatggaa aaaataacct gcatactttt tttgcgataa
    28201 acgagtggct gcaatgtaca agtaaccgaa agtttcttta gaaagctgct taatgaaatc
    28261 aagctccatg atacataaag agtaaaaaag gccaacaaga accagcctaa agaattgttg
    28321 aatacaagag caaaaagcac aatgatgaaa tataccagaa ataacacaaa attaatgaaa
    28381 taatctttga ttttttccat tgtctacctc aatctacctc acaggaatag gaatttgtgc
    28441 aacaagagta tgaatgattt gtttcttttc gtcttccttc aatgaacgat cgtaaaaaat
    28501 caggcgatgg gaaaacacgt aaggaactaa atcctgcaaa tctttgggcg tgacgtaatt
    28561 tctcccatgg attaaagcat aagcttttgc tgcctgaata aaggaaaggc ttcctcgtgg
    28621 actgatgcca agctggatgg cagcatgact tcttgttgct gacactaact gcaaggcata
    28681 agatgccact tcgggactga caaagacatt cgccacattt gtttttattt cttctatttc
    28741 ataactgttc aatacttcat ttaagttatc caacactttt tcgaatttat ccaaaagtag
    28801 caaaagttcg tcatcaaaag aaggataacc gatttgtagt cggaataaaa aacgatcgag
    28861 ctgtgcttcc ggcaaaggat atgttccttc gtactcgatt ggattttgcg tagctaagac
    28921 aaaaaaatga ttgtctagag ggtatgtatt gttgtcgatc gttgcatgat tctcggccat
    28981 tgcttctaaa agtgctgact gagtacgagg agtcgtacga ttgatctcat ctgccaacaa
    29041 gatcgtggta aagatcggtc ccggacgaaa ttcaaactct tttgattgtg aattgtatac
    29101 ggaaaaacca agaatatcac taggcagcag atcaggagta cattggactc tgctgaaggt
    29161 tccctgtaca gcttttgcta gtgctttgac cagtaacgtt ttacctacac cgggaatatc
    29221 ttcgaataag acatgaccac ctgctaatag ggccacgatc atcaattgga tagttgaacg
    29281 tttgccgatg accgcttttt ctaattctta tatcaactga tcgatttttt gtttggattc
    29341 ttctattttt tcttccattt tcacatcacc ttctcataaa atcgctattt atttcattgt
    29401 aatggaaggg aatacatcct acaagcaaag tgatcttatt atgttttttt aaagtacatt
    29461 cgttttgaaa tgagaatacg attaaattga gtaaaataaa aaataatgag gaagatttgc
    29521 gtatttaaat aaatagaaag ggagtttttt atctttttag gagtgaactc attttgatca
    29581 aacaatatgt ttagaaatca ggtgagaaat gatcgatgag atggcaaaaa ctattatcaa
    29641 aaaatgcaca gaatttattt tcactaatca atctaatgaa agatttgatc tggcgaaaag
    29701 ctgatttagc tgaagaactg aaaatcgatg ttaaaacagt ggatcggcat ctgaaacgtt
    29761 tggcggaaag cgactgtccc attcagctga ttcttcataa gaaaaccgtt cagttagttt
    29821 atgatcaagg atatagcgaa ccttatctct tgtttgtttt tctgatccaa tcacccccgt
    29881 tttgtttact gaaaaatctg ctattaaatg aaccttacga gcagaagtac gatcgttcta
    29941 cgcaagagcg attgagaaag tggtttggcg agtaccacct ttctttttct tataaaaaag
    30001 cacagatctt tggttcagaa agaaaagtaa gatatctgct gttttacttt ttgaaagatt
    30061 ttccgcattt ttttgcagat gatggagagc agtccttttc tgagatgttc atccaattat
    30121 tgaagcaaag aaaggatccg gcagaaatcg agattttagg agacagtcga acactattct
    30181 ttgaattgtt tcctgaattg ctatttagag aggagcagat tttgccaata aaagaaaaaa
    30241 attatctagc aattttgcgc ttgagctgta accagattac tgaaaagcgg tggaaacgga
    30301 tagaagagac acaagactat cggaaattga cgattgtgac tgaaaagctt gattcgctgt
    30361 tttggctgaa tagcgaattt gagagcgaaa gcaaatcaaa gctgataaaa gtactttacc
    30421 aagaatggct tcgttacgta attggtatcc atgaccggct gatagaagaa gttcgttatt
    30481 atcaggaaac attagaaaaa tttcccaatt ccaaagataa actatatcaa tttcggctag
    30541 cgatccaaca agcacttgca caccccgcag tagagtgggg gatgctttta ctgaaaacac
    30601 tccaggaacg agggtattta gatgtccacc ctcctgaagt gaaaatcgtc tttttttttc
    30661 gctatgaaca ccaagaagca agaagacttg ctggggtact gaacaaaacg ctaagacatc
    30721 gaaaacggat ctctatacat gtatgtactc gaaataagcc gcctcggtat gcagatctag
    30781 tgatcaccga tcattttttt ccactagacc ataacgaaga tcaaaaaacg tatctgtacc
    30841 atccttcatt tggtatcgaa agacttctta ccgatatcaa ttactggtta ggcaaaaacc
    30901 gataaaacct ttggaaaaaa gatcaaaaca agtcagtctg tgctataata aaaagcgatt
    30961 agtaaaggag gaaatccatt ggaattaaag aaattagaag ttcccacatc ttcgatcaca
    31021 gaagtgaaac gatcgccgat ggatgtattt gctcaagcgc gagacgcggg aactggcgta
    31081 tatatattta atcgtgaaaa agtagctggc gtgatgctga cccaagaaca atacaaaacg
    31141 ctcttgcaag aactagatgt ccttcgtcaa acagtgaaag tggagaggaa agaaccaaaa
    31201 gaaataaacg aagaacctat taacaaaaca gcttccgtcg tatccaatgc tgaagggatg
    31261 gaagagctgg cccaaacatt acagcactcg ctcattactg gtgtgacgat tacagcaaaa
    31321 aatctagatg aacgaatggt agcactaggt tttattacga aaaagaccgg atttggcgga
    31381 gtcgtggata tgcttagcga gttgatggaa actgggaaaa tcaactacca gttaagaaga
    31441 aaaccgcaaa atcggacaat catagctgaa atcataggag agcaaaacag tcaatctcaa
    31501 ttgatggata aaatcatcat caagaaaatt tttctacatg agctataaga aaaccatgtt
    31561 cgcacttcta ggcaagccag gctttgaaga ccttgccaaa gaattgaacg aacgccttta
    31621 agataagggt ttttcgctag atttaaaaaa tcagtgatag aaacatgttc gcttataaat
    31681 aaaattagtg atagttttag tgatagtgag cttaaaaaag cactcaattt gagtgctttt
    31741 ttatttgctc ttctatatag ttttctagtt gattcattga tctttttttc acttcgggag
    31801 aaatatgggc atagacttgt gtagtggaaa catctttatg acctaaaaaa tctttgatat
    31861 cttctaaagg tattcctgct tgtctcgttc tagctgaaaa agcaaaacat aatgttggtg
    31921 ttttcctttc agcatatcta tcagcaagat gtagagtagg acaacctcgt gagccaaaag
    31981 tcctgtgcaa atcgagttac aacacacaca tgataaagaa acttttgaaa agaactataa
    32041 agtatacgtt gaattggcaa gagattcagc aatgaaatat ggtattccat taacattaga
    32101 cactccttat aaccaaccag gaatcaaatc gcatttatgg gtaacacaaa atatttgggg
    32161 cgatcataca gatccttacg gttatctttc tgaaatgggt gtaagtaaag aaaaactagc
    32221 ctatgatttg gctcatggtt ttacggatga taatccaact acttcggagg ataaaccagt
    32281 aattgatcca actcgagcag gtgctgcaaa ttctacgctg acagatggaa caaattacgc
    32341 ccacattgat cagttcggag aaatcgaaaa cgcaaacttg catgtagctg gatggcacat
    32401 tgctaattat aaatacgagt atattttcat tatggactac aatactggaa aagaactagc
    32461 tagagtaaga gctgatggga tttatagacc agacgtaaat caagcttata atactttagg
    32521 aaatgttggc tatcatgtat ctttcaatat gcgtgatttc cctaataaga aagtatacgt
    32581 catgatgcgt gcgacaaacg atccagaagg aaatactaag ggtggagcac aagattttca
    32641 tgacaagcgt tggtatttaa atattccaca acgataaaaa atccccccct cttt
//
LOCUS       DAYYFD010000026        32233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000026 DAYYFD010000000
VERSION     DAYYFD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.026"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(171..551)
                     /gene="yajC"
                     /locus_tag="ACHA25_001090"
     CDS             complement(171..551)
                     /gene="yajC"
                     /locus_tag="ACHA25_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8762396.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(648..1793)
                     /gene="tgt"
                     /locus_tag="ACHA25_001091"
     CDS             complement(648..1793)
                     /gene="tgt"
                     /locus_tag="ACHA25_001091"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8762397.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2011..2709
                     /locus_tag="ACHA25_001092"
     CDS             2011..2709
                     /locus_tag="ACHA25_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8762398.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2877..5516)
                     /locus_tag="ACHA25_001093"
     CDS             complement(2877..5516)
                     /locus_tag="ACHA25_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8762399.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6135..7166)
                     /gene="queA"
                     /locus_tag="ACHA25_001094"
     CDS             complement(6135..7166)
                     /gene="queA"
                     /locus_tag="ACHA25_001094"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8762400.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7359..8534
                     /locus_tag="ACHA25_001095"
     CDS             7359..8534
                     /locus_tag="ACHA25_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8762401.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8538..9179
                     /locus_tag="ACHA25_001096"
     CDS             8538..9179
                     /locus_tag="ACHA25_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762402.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9176..10093
                     /locus_tag="ACHA25_001097"
     CDS             9176..10093
                     /locus_tag="ACHA25_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8762403.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10090..10761
                     /locus_tag="ACHA25_001098"
     CDS             10090..10761
                     /locus_tag="ACHA25_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762404.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11302..11790)
                     /locus_tag="ACHA25_001099"
     CDS             complement(11302..11790)
                     /locus_tag="ACHA25_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8762405.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11991..12257)
                     /locus_tag="ACHA25_001100"
     CDS             complement(11991..12257)
                     /locus_tag="ACHA25_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8762406.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12257..12520)
                     /locus_tag="ACHA25_001101"
     CDS             complement(12257..12520)
                     /locus_tag="ACHA25_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8762407.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13041..13181
                     /locus_tag="ACHA25_001102"
                     /pseudo
     CDS             13041..13181
                     /locus_tag="ACHA25_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13323..15146)
                     /locus_tag="ACHA25_001103"
     CDS             complement(13323..15146)
                     /locus_tag="ACHA25_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8762408.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15744..18449)
                     /locus_tag="ACHA25_001104"
     CDS             complement(15744..18449)
                     /locus_tag="ACHA25_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8762409.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18755..19702)
                     /locus_tag="ACHA25_001105"
     CDS             complement(18755..19702)
                     /locus_tag="ACHA25_001105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8762410.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19892..20161
                     /gene="rpsN"
                     /locus_tag="ACHA25_001106"
     CDS             19892..20161
                     /gene="rpsN"
                     /locus_tag="ACHA25_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8762411.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20337..20699)
                     /locus_tag="ACHA25_001107"
     CDS             complement(20337..20699)
                     /locus_tag="ACHA25_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8762412.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20710..21831)
                     /gene="alr"
                     /locus_tag="ACHA25_001108"
     CDS             complement(20710..21831)
                     /gene="alr"
                     /locus_tag="ACHA25_001108"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8762413.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21847..22197)
                     /gene="acpS"
                     /locus_tag="ACHA25_001109"
     CDS             complement(21847..22197)
                     /gene="acpS"
                     /locus_tag="ACHA25_001109"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8762414.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22327..23838)
                     /locus_tag="ACHA25_001110"
     CDS             complement(22327..23838)
                     /locus_tag="ACHA25_001110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8762415.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24160..25512)
                     /gene="murF"
                     /locus_tag="ACHA25_001111"
     CDS             complement(24160..25512)
                     /gene="murF"
                     /locus_tag="ACHA25_001111"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8762416.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25665..26741)
                     /locus_tag="ACHA25_001112"
     CDS             complement(25665..26741)
                     /locus_tag="ACHA25_001112"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8762417.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26937..28682
                     /locus_tag="ACHA25_001113"
     CDS             26937..28682
                     /locus_tag="ACHA25_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8762418.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28962..29399)
                     /locus_tag="ACHA25_001114"
     CDS             complement(28962..29399)
                     /locus_tag="ACHA25_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8762419.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29383..30756)
                     /locus_tag="ACHA25_001115"
     CDS             complement(29383..30756)
                     /locus_tag="ACHA25_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762420.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31078..31566)
                     /locus_tag="ACHA25_001116"
     CDS             complement(31078..31566)
                     /locus_tag="ACHA25_001116"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8762421.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31588..32190)
                     /locus_tag="ACHA25_001117"
     CDS             complement(31588..32190)
                     /locus_tag="ACHA25_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8762422.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9219 a         6886 c         5948 g        10180 t
ORIGIN      
        1 caggtgtttg tatagaaaaa tatataaagt tatttattat tatttatatg acacacccga
       61 ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg aataaaaaat
      121 gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac ttatttttct
      181 tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc aactgtctca
      241 gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac agtccctggt
      301 ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca
      361 actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc gatcgttacg
      421 actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg ttgtttcttt
      481 tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac aagcattggt
      541 aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact ttagcagaaa
      601 aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta gaagcttttt
      661 gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc aagcaaatta
      721 tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta taaattgtgg
      781 taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg gatataggca
      841 cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg gaaatcatgg
      901 gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt gccatttcga
      961 gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac accatcgatc
     1021 aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg gatcaacggt
     1081 gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga taacccgccg
     1141 attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg acgaagatct
     1201 tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt agcatgagct
     1261 ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac gtagtcgtaa
     1321 ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc caatttattt
     1381 tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa gtgatttttg
     1441 aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac ttgaaagccg
     1501 cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc gccagcttct
     1561 tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa aataacgcca
     1621 gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa tgttcctact
     1681 ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg
     1741 ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt cattcatttc
     1801 cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt caagtgcttt
     1861 ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta tccttttttt
     1921 atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac atggtatatt
     1981 ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa
     2041 gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct aatccctaca
     2101 ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt tgccaccacc
     2161 atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg tagttcagga
     2221 ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg tatttcttgg
     2281 acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa agatgcattt
     2341 cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct gatctcgatc
     2401 tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag ctattcttat
     2461 tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac acctaaagta
     2521 ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca acttttctgg
     2581 ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg tattggttat
     2641 ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca attgccaaaa
     2701 gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca ccgtttatcg
     2761 agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca acattcatgt
     2821 cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac agttaatcat
     2881 tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac cgcgattggt
     2941 acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac taggccgaat
     3001 agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc
     3061 atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg gctgctgcgt
     3121 acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac agtttgaccg
     3181 acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc taaataataa
     3241 ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg caataaacct
     3301 cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc gtgttccgct
     3361 ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac aaacagtagt
     3421 tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac aataaagatc
     3481 tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg ataaaccgaa
     3541 cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa aatcatatct
     3601 gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac gttacttgct
     3661 ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc acccgtacgt
     3721 tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata gacagagaga
     3781 gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga tcctgaaagt
     3841 gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc agtcaccaca
     3901 tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt gattgctcct
     3961 ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg taagtctttt
     4021 tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata accaacagca
     4081 atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg ttctacatcg
     4141 cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga gatgtatttt
     4201 tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt
     4261 actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc aacagccctt
     4321 acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc ttgctcgacc
     4381 gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt cgtcattgaa
     4441 tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg catttcattt
     4501 tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag cgtttcaact
     4561 gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt
     4621 gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa ggaaatcgaa
     4681 gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa tactaaagcc
     4741 gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg gacctgtaaa
     4801 ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat ttctgtagcc
     4861 atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca tcctttaaat
     4921 accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc tgcatctttt
     4981 tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg tgatgcttcg
     5041 atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat atcacccact
     5101 accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt tgccgtttgt
     5161 ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc
     5221 aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa atccccatgt
     5281 tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat gatcgttgta
     5341 aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc ctcatcgaat
     5401 ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa cccagtatct
     5461 ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc ttccatccta
     5521 ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac atcctttttt
     5581 tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa atttaaagtg
     5641 cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa ggaggatacc
     5701 atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt tgttagaagt
     5761 acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga
     5821 tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg
     5881 taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa
     5941 acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta tgatggaaag
     6001 ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga gcttatcgag
     6061 agctcggctt tttctttgtc ataaaatctt tgtaaagctt ttttgttatt caaaactagc
     6121 ggcttttcat aaccttattt cacgaacatc gcatctccaa aactaaagaa acgatagcgc
     6181 tcttcaatcg catgctggta agcagataaa gtcaattctc ttcctgcaaa ggcactaact
     6241 aacatgacta aagtcgattt tggcaaatgg aaattagttg agaaagcttc taccactttg
     6301 aattcatagc caggcgtaat gaaaatatca gtccatccgc tgtctgcttt gatttctcca
     6361 ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac agccacgatt
     6421 ttcccgccat ttttacgtac ttggttcaat cgctcagccg cttcttccgt caaacggtag
     6481 aattcactat gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct
     6541 aaccctacat gtagtgtcag atagactaat tcaacaccct ttgcttcgat ctcatcaagt
     6601 aattctttcg taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt ttctttcgca
     6661 tagacagttt gatagcgatc tgggtcttcc agacgttctt tgatatatgg aggcaatggc
     6721 atttctccca atgattcaag aatctctaga aaaattcctt catactcaaa gtcaatgatc
     6781 cgtccgccgt gttccaattc ttctttcact actgcttgca atctgccatc accaaaagag
     6841 attcttgtcc caactttggc acgtttcgca ggtttgatca atgtctccca cgtatctcct
     6901 tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag tatctggttt ttctccatat
     6961 aaacgagcag gcagaactcg tgtattattc ataactagcg catcaccagg ctgtaactct
     7021 tcaatgattt catggaaatg tttatcttcc atctctccag ttttccgatc caaaacaagt
     7081 aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc cggcaagtca
     7141 aaatcaaaat cttctgttgt taacatatcg tcactcactt tctatcatgt gcttcatcat
     7201 tctatcatgt ttttcgttct gttggcaaaa tcagcacttt ttcacttcta ttttttgttt
     7261 tcctaaataa aaaaactacc ctatttgtat tttttcattg ttttaggcat actagtaata
     7321 gatagttctt cattgaaaat agaaaaagag gtggcaccat gattgaattt caaaatgtgt
     7381 caaaaattta taaaggtgga aaagtcgctg tagaggacgt caatctttca tttgaaaagg
     7441 gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg atgcggatga
     7501 tcaaccgaat gactgatcca acacaaggga aaattttgat tgatggaaaa tccatccaag
     7561 aaattgatcc agtggaattg cgacgtcaga tcggctatgt tatccaaagt attggattga
     7621 tgcctcacat gacgatccgt gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc
     7681 aagaagaacg gaataaaaca gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga
     7741 tgttagatcg ctacccacat gaattatctg gtggtcagca gcagcgtatt ggagtcgttc
     7801 gtgcccttgc agcgaatcaa gatatcatct tgatggacga gccattcggt gcgcttgatc
     7861 caatcactcg tgattccttg caagaccttg tcaaagattt acaagagcgt ttgggaaaaa
     7921 cgatcgtatt tgtaacccat gacatggatg aagcgattaa actagcaagc cgtatcgcca
     7981 tcatgagtga aggccgtgtg atccaatttg atacaccaga aaatatttta cgtcatcctg
     8041 caaacgattt cgtagaagag ttgattggag aagatcgcct actacaagcc aagccaaatg
     8101 cgacaagagt cggcgaagtt atgctaaata cagcaatcac gattacaccg gaaaaatcat
     8161 tacaagaagc aatcaaactg atgagggaaa aacgtgtaga tacgttgtta gttaccgata
     8221 attcgaatgt attaaaagga tttatcgatg tagaaacgtt gaataaaaaa cgaggaaaag
     8281 tatccagtgt cggcgacatt ttgaataaac atgtttttta cgtaaaagaa tccgcctatc
     8341 ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa atacgtgccg gtcgttgatg
     8401 aacagaacaa agtcgtaggt atcctgactc gtgcatcatt agtggatatc gtctacgatg
     8461 tgatttgggg agaagatccc gcaactactg atgttgcaga gtcagctact cctgaagaat
     8521 ctaaggaggt ctgacctatg cagcagtttt ttcatcagca cggttcagaa attctctcaa
     8581 aaggttttga acatctttat atttcaggac ttgctttgat cattggtatc atcattgctg
     8641 taccgcttgg tgtcttactg acacgttttc caaaagctgc cacgattgtg attggcttaa
     8701 ccagtgcctt gcagacaatc ccttctcttg ctttattggc attgatgatc ccgctatttg
     8761 gcgtaggtaa attacctgcg attatcgcac tctttattta ttcattattg ccgattttac
     8821 gtaatacgta cattgggatg aaaaatgtcg attggaatta tcgggatgtg gcaaaaggca
     8881 tggggatgac tgagttccaa tccatttttt ccgttgaatt gcccattgct atgccaacga
     8941 tcatggctgg tattcgttta gctgccgttt acgtgattgc atgggctaca cttgcctctt
     9001 atatcggcgc tggtggttta ggcgatttga tttttagtgg actgaataat taccaaccag
     9061 atctcatttt ggcaggtacg atccctgtaa caattttagc actattggct gattggcttc
     9121 taggtctctt ggaacaccgg ctgacaccga tcgcacttag agaggagaat gaagaatgaa
     9181 acgtatacta aaaatgacca tggttctttt ctctcttctt atacttgcga gttgttcctt
     9241 ccctggtctt gccagcaata cagatgaaga caccatttcg atcacaggtg gaatcacttc
     9301 agaagcacag atcttagcaa gtctagttgc cggaatggtt gaacattata cagataaaaa
     9361 taccgcaatc atcaacaatc tggctactac aactatcaac catcaagcga tgatgaacgg
     9421 agatgcttca atttcagcgg ctcgctacac aggaacagat ttaacgacta cattgaacct
     9481 tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat ttgagaaaag
     9541 atacggtcaa acgtggttcc cgtcctatag ttttgaaaat acgtatgtct ttcttgttcg
     9601 aaaagacacc gcacaaaaat accatctatc taaagtcagc gatctgaaaa atgtagcgga
     9661 tgaattagta gctggtgtag atacttcttg gatcaatcgt aaaggagacg gttatgacgg
     9721 cttccaagaa acttatggct tctccttcaa ttcgatatta cctatgcaga ttggacttgt
     9781 ttatgacgca gtcgaagcag gaaaaatgga tattgttctt ggctactcta cggatggacg
     9841 gatcgccagt tatgatctag tcatgttgga agatgatcgt caattcttcc caccatatga
     9901 tgctgctcca gttgtagaca acaagatatt agctgatacg cctcatttaa aagaagcctt
     9961 aaataagtta ggtcatacca tcagtacgga aaaaatgcag gaattgaact acgaagcaga
    10021 taataatcta gtggaaccat cagtagtagc acaacgtttc ttacaagaaa accactattt
    10081 tgaagggaag tgaagcaatt ggataaaatg aatgtctttc aacaattttt ctattatttc
    10141 caggaaaatg gctcctatat ctttgcacaa tttatccgtc acttcctgat ttctatctat
    10201 ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga tcatgatttc taggagacgt
    10261 aaattagcaa actgggtgat tcgtttggcg aatatcatcc agacgatccc ttctctagcc
    10321 atgatctcca ttttaatcat tggattaggc ctgggagtca atgtcgtgat tgttactgta
    10381 tttttgtatt ccctcttacc gatcatcaag aatacgtata ccgggatgat ccaagtagac
    10441 aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg ctagacaacg actattcatg
    10501 gtagagttac cactctctgt ttctgttatc atggcaggca taagaaacgc gttagttgtt
    10561 gccatcggga taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg agatatcatt
    10621 atccgaggaa ccaatgcaac agagggcaca tcgattattt tagcaggtgc attgccaact
    10681 gcattaatgg cgatcatcac ggactggctt ttaggtattt tggaaagacg gctagatcca
    10741 gccagccgta catcacgctg atgttattgt aaaaaacaaa agcttctgcg accatttgtt
    10801 ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa taatccactc tgattcttcc
    10861 ctccttatgg gcaaagagtt gcttttctta gctatttatc cattccgtat actccaactt
    10921 catactgcca acgggttcat atctgcatcc accgactaag tttttttctt atccgctaag
    10981 gcattttgcc aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa
    11041 atactcgtct gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt
    11101 catttaagcc ttttccatca gtcctcgatt gttcgatgcc ttcagctgca tcatcaaaac
    11161 tggtgactgc cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct
    11221 catccttggc taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt
    11281 cctaaaaccg gtacacgatt cttatggact gcatccgtcg caactagact tggcgatacg
    11341 atgatccttc accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga
    11401 aaagacattg gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt
    11461 catggccgaa agcgccacga ctttaacttc ttccgtttga gtgacattca tgttcggcaa
    11521 tgtcgctttg gatacccgct ctgctaaagg aacgacactg gtattgtatt tcgtatctgt
    11581 atcttttcca aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg
    11641 attgtccatt gcttttttca acatactttt ggctgtttct cgataaacga catttccaat
    11701 tgtcttcgcc tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga
    11761 tgctccaatt ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg
    11821 tcaacattcc attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa
    11881 ttaaagaaat catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct
    11941 ttttggggag actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa
    12001 tgtgtccagg cagaataaat ttctacaact ttattttcct catcaacttt gtaaaccact
    12061 cgatgctgtc tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca
    12121 aaagactgcg tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt
    12181 ttctttaaat tcgtttgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg
    12241 actgtatagt tactcatcaa agagcttccc aatcaatatc atcgatattc gtaaagctac
    12301 tgtcgtcttt ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg
    12361 tttcttgaat cgctctccag tcttccagcc caatcaatac agcattgttt ccactacgat
    12421 cactaataat ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat
    12481 ttttacgagc agtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg
    12541 atataattat agcgtacttg attaagtacg tcaaaaaaat acaataaagc acttaaattc
    12601 agcaaacttt tcctcttttt tacatagaga aaaagacccc tttttaggga ggatctttgt
    12661 ccacgctcac ttcttactcc tttacgacta gcggtagcca aacgtaaagt aattggtaac
    12721 aacgtatcat ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca acaacaccaa
    12781 tacacgaagg ttcctcgttg cagtgtgact tgattgatat cttgaataac ctgaggatac
    12841 gcacgaaaga tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc ttctactgcc
    12901 tcaaaaatga ttgcctcttt cgtctcctgt cattcatgtt cactatcgat ttgatacttg
    12961 cttagaccgt tgggtttgtg aatcaccaat taaaaatggc actgagttat ggtaacaaaa
    13021 aatggtcact tgacgaggct gagattccta cttatgaaga cacatggcac ttaagagtcg
    13081 aggtaaagga agaagctgaa aatataggag gctgtacatt atttggcaaa ggattcggaa
    13141 attatgatca agacatcaaa atcaaaatgt tttacagcta aataggaaga ataataacat
    13201 ttcatacgct gttcccatct ttaatggctt atagagggca cctttagctg agaataagac
    13261 aaaacagacc ctctagcttc aattaagagc tagagggtct gtttttgatt ttaaaacttg
    13321 gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg ccaagacaat
    13381 cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg tctgtttggt
    13441 acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt tttacttacg
    13501 atatctacat aacggataat tggattttca atcgagcgat aagaagagtg gacgattgaa
    13561 aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc tttttcgaca
    13621 tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg tgcgtaattc
    13681 aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt attcccgtca
    13741 taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt tttataatga
    13801 tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa aggccaaatg
    13861 tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa cgcacccgta
    13921 atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt gacgaccatc
    13981 cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg aatcaagcgt
    14041 tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag tgtgataatc
    14101 ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat gaatttatct
    14161 tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc agcaactgct
    14221 aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc aaagacagct
    14281 ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata ccaatagttg
    14341 ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt caatgtagca
    14401 gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat cgcctccacc
    14461 ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat agaaacaccc
    14521 ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt cacgatatta
    14581 tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac cggaaataat
    14641 aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag caaaacgatt
    14701 gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt gatcgcttca
    14761 gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa agagccaccg
    14821 gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc tcccccgcca
    14881 tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa taaaataata
    14941 acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc gactaaaacg
    15001 acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga caataatgcc
    15061 aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa aggcttacct
    15121 actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat tttacttcaa
    15181 gttttatagt cgaaaattta tagtaaacaa gatgttatag tacgctcact gaccttaaaa
    15241 gtcaataaac tttaagaaaa taattggaaa aaaacagaaa aatatcaagc gctttttcaa
    15301 acgctttaat acagcaaaaa tctaacattt ttttatgggc tttttctttg aattctataa
    15361 taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat cttaactttt
    15421 ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag catagcgcag
    15481 ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa taagcccaac
    15541 caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg atgcagagca
    15601 gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt attagctcaa
    15661 aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact caggtcaaaa
    15721 cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc cgcttctttg
    15781 aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat tccaaagaca
    15841 acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata aattcctcct
    15901 gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt gatcatttgg
    15961 aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat cgacagtact
    16021 aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa gacatagacg
    16081 atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac cggattttgg
    16141 acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg cgcaatcgcc
    16201 attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa attgtttcgg
    16261 tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac agcgccgacc
    16321 cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc gattggataa
    16381 agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt tgttgcgtct
    16441 agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg
    16501 tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat caagtccgca
    16561 gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt caatgtaccg
    16621 gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc caatcctaat
    16681 ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt gatgccattg
    16741 acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc ttgtccaatt
    16801 gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac agttgctttc
    16861 gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca
    16921 gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact gatttgacct
    16981 gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat ttgatctagc
    17041 aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc agataggcgg
    17101 ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat tggctgtaat
    17161 ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt atcgattgca
    17221 ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat gtttttcttt
    17281 tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat taacgaacct
    17341 gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt tgtgatgctt
    17401 ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag atcacttgcc
    17461 tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat gatcgtcaag
    17521 ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc aaaatcgccg
    17581 tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc ttgcaaatca
    17641 aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc aacgactttt
    17701 tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt attcagtttt
    17761 tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta gccgtcgatc
    17821 gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat acttactagg
    17881 ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga actggctgtt
    17941 ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc ggtgattttc
    18001 ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt cttgatatca
    18061 ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat acctgcgtaa
    18121 tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa ttgccatcct
    18181 aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac ttctttacct
    18241 tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc agtatttcca
    18301 tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat gatcatgatc
    18361 gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc aagcaaatat
    18421 aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt ttcagctgaa
    18481 ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa gtcgaatttt
    18541 tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa taattaagga
    18601 ttacagcctt tactttatca tgaaataaaa gggaaagata attttatgcc aaagttttta
    18661 tctattatac gtatacgcaa aaaagctaaa acattttttg tctttgcccc tacaaaaatg
    18721 tttcagcttt tttcgttttt tattcctgta tttcttattt ttcgaatgac aggatgattt
    18781 tcccagcagg aggatgtcct tcaactagct gatgtgcttg gatgacatgc ttaagtgaag
    18841 cagacagatt cattgaaata aaaacatgaa aggctccttt ttggtaggct ttgattatgc
    18901 cagcaaaggc ttctgcatct agatattcct ttcttggaac aaacgattca taaaagcctt
    18961 cctttttttg ccgcaaatct agatctggga ggtcatttaa tgcaacatac ctgccgcctg
    19021 gtttcatgat ctggatgccg gtttctcctt tgatacttcc tttcgtcgca tcgattacaa
    19081 ggtctgcctg atcagcaaat tgcagacctg gatttgtttt atcataagca gcaaatgctg
    19141 aagctcctaa tttttttacc ttttcctcat ttttactgga tgcactggtt aagattcgga
    19201 ttcccttttc atggagaagc tggatcagac ttgacccgac cgcacctgat gctcctagta
    19261 tcattacagt atctgtcggc tggatttccg tcaaatggtt gattagatta taagccgtaa
    19321 ttcctggcgt gaccattccg gctgcttctt cccaactcat tttatctgga atttttgcta
    19381 cttttgctga tggcaacacg acttcttctc cgtaagtccc gccgactgca tggacagcta
    19441 ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc
    19501 catcattacc tggtacataa gggaatttca atgttcgtat ctctttcatt tctcctaaac
    19561 gcaaggcaac atcataagga ttcacactga atgcttttat ttctacacga acatgtccag
    19621 aaccgacttc tctgggatga gcatcgattt cttcaaaaac atcttgtgct tctccgtaac
    19681 cgttaattgc aaatcttcgc atggtcacca tctccctttt tctttaagtt tacgtttatg
    19741 cctttatttt gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa acaaaatagt
    19801 tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat tacgatttac
    19861 gctaataaaa aataattgga ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc
    19921 aaaaaaacag caagaaacga ttgaacgcta tgctgcttta cgccaggaat taaaagcgca
    19981 aaaagattac gaaggcttgc gtaaacttcc tttagatgct cgtccagaac gattgaaaaa
    20041 tcgtgattta atcgatggac gccctcgcgg atacatgcgt aaattcggga tgtcccgtat
    20101 aaaattccgt gaattagcac ataaaggttt gattccagga gtaaaaaaag ccagctggta
    20161 atccaagtct ggctgtaatg taaaggagga gggcgcatgg accactcctt ggatcatcaa
    20221 aaaagatacg ttcgcttctt ctagcagaaa tgctggtact agaagcagac gtatcttttt
    20281 ttacgcacag ctggtccttt tttatatgta aaggctgcat ggttacttcg ctatatttat
    20341 ttagtaacag gagtcagtgc ttcgataccc acactaattt tcaatgctcg gtcaacttct
    20401 gccataatgg gtcgatccaa atggcagacc ttttctttca atcttgattt atcgatcgtt
    20461 ctgatctgct ccaacaggat aacggagtct ctttctattc ctgttccagt agctttgatc
    20521 ccaatgtgag tcggaagctt aggtttagcc atttttgccg ttatggccgc aacaatgatc
    20581 gtcgggctaa aatggttacc tagattattt tgaataacca aaactgggcg tgttccgcct
    20641 tgctccgatc ccacgactgg agaaagatca gcaaaataaa tatctcctct ttttaccata
    20701 ggatcctcct tattcttgat attctcttgg tatccgctgt cctaatccgc aagccacttc
    20761 atagtggatc gtacctaatt gatctgctat atcctgcata gtgatttctt tgtctgcatt
    20821 tttgccgata agcgtcactt ttgttccgac aggaaattct tgaggcaatc ggatcatcag
    20881 ttgatccata catacacgcc caatcgtttc acagtagttc ccttctacca atagagaaaa
    20941 accctgcatc tttcgtggcc agccatctgc ataaccgatc ggtatcgttc cgatccactc
    21001 tgcttcagga gtgatatatg tttctccata gccgattcct tcacctgctg ggagtttttt
    21061 gacttgaatc aattcagaaa ccagctcaag tgctggctgc aatggataaa cttccggtag
    21121 tgcgtgccca gagggattaa gtccgtacat tgctactcca taacgaatca tattgccgat
    21181 tgtttcatca tgccataaag ccgttgcact attgctgaca tgtacatatc gaggaagttc
    21241 gggaagtttt ttcagcactt ctataaagcg ttctttttgc agattccaat atgtatcatc
    21301 tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc cattctaata cacgactttc
    21361 tttgatgaat gcagctgcct cttttacttc ttcaggtgta caaaatccga tacgacccat
    21421 cccagtgtct gcttttagat gaatcgaaag aggcgtctct agcttacttt tctctaaaac
    21481 agcagctgct gcttgcaacc attcccgtgt acctgcagtt actgataaat catgagcaac
    21541 agctacagtc agtgcgtcgt aaggaaccat attcaagata aggatgggct gagtaaatcc
    21601 agcttctctc aactcgactc cttcatctaa atttgaaaca caaaagcccg aagctccacc
    21661 tgcaactgct gcctcagctg tttcaatagc tccatgaccg tatccgttcg cttttactac
    21721 tgcaaacaat tcttttcctt gcggaagtct tgcaacttca tttgctacat tttcagttat
    21781 agctttttta tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc
    21841 ttctttctag tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat gagtaatact
    21901 gacccataca ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat tttcattttt
    21961 taaaatctcg acatcttgaa aggtaacttt tccgatccca gttccccaag ctttagagaa
    22021 agcctctttg caagcgtaac gcccgcctaa aaattctact tggcgatgaa aaggcaagga
    22081 ctggaatagt ttcatttcat cagaggtcaa gattcgtgca atgaattttg gtttttcttc
    22141 gatcagccga gtgatcctag gaagttcgac tgcatcgatc ccaattcctt ttatcatatg
    22201 cttttctctt ttcaataaat ttagaacact ctcattctac caaattataa ccattttgcc
    22261 acaaataagt ccacaaataa caaaaatgaa agtttctctt aaaaaagaga aactttcatt
    22321 ttatgtttaa tcgttatttg aacggattac aaatccgcgt tttttatcat tgtgacgttt
    22381 accgccatct ttttggctgt tattattttt gctataaccg ccttttgatt tgtttccacg
    22441 ataattaccg ccgtcacgac gattacggtt gtttccgcca ccacgtttgc catttttatt
    22501 gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg atcttcactg gcactgcatc
    22561 agctggatct tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg cagaatattc
    22621 ttctaataat ttatctgctg tttgcaggta tttatctaga ccattttctg caagttttgt
    22681 ttcgatttgt tcaactgcag cacctaattg acctttgaat gcttcttttt cggttggtgg
    22741 acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt agtccatttc
    22801 atttggtgta acgaaagtca cagacatacc gccttttccg gcacggcctg tacgtccgat
    22861 acggtgaacg tagctttctg gatcttgagg gatatcatag ttgtacacat gagtgacacc
    22921 tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca aggatatcta aatgaccgct
    22981 cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac aaatcgccat ggattccttc
    23041 tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc gttttgtacg
    23101 tccgaagacg attgttaatt caggtgtttg aacatcaaac aagcgagtca tgatatcgaa
    23161 tttttcgtat tcttttgcgc ggacatagta ttgatcgatc aagtcagctg tcatttcttt
    23221 cgctttgatc ttcacatgat gtggattttt catgaattta acgccaatat tcttgatagc
    23281 tggcggcatc gttgctgaga aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat
    23341 tttttcgata tcttccaaga agcccatatt caacatttca tctgcttcat ccaaaactaa
    23401 tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca tacgtcctgg
    23461 tgtacccaca acgatatgtg gacgatcttt caagccacgg atctgacggc caatatctgc
    23521 tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc cctaaacgat ataattcttc
    23581 ttgtgtttgg atagctaatt cacgtgttgg tgcgataact aaaccttgta attcgtgacg
    23641 atctggatcg attttttcta acattggtaa tccaaatgca gctgttttcc ctgtacctgt
    23701 ctgtgcttgt ccaataacgt cttttccagc taatgctagt ggaatcgttt ctgcttggat
    23761 aggcgttgct tcttcaaaac cagcgcgttc gactgatttc aacaattctg gtgataattc
    23821 tagttcttta aatttcaaat gaatcctcct atatggttgt taactgagga tgtagctttt
    23881 ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca gtggccacgc
    23941 acgtttttat ccattcttag cccctgtggc accaccgaaa aatcactttg cgaagtttag
    24001 gcattagaaa agccaaacgg gtcgcgttca gctttatcat tattatgaca tctggcttca
    24061 acaagttgtt tctatggcaa aaccagtcca atttctcttc atctttcggc aataggttat
    24121 tctaacatag tttttcgttt tctgcaagtt tacagatttt tatttcattt gctgaagttg
    24181 ttctaccact tcaaataagc ccatgctgtt gctgcctttt agcataatac tatctgttgc
    24241 ttcaagattt tgtttgatag catcaatcaa tttattcttt tctttttcaa aatagaacac
    24301 aggtagttgg gggtattttt ggttcaattc ttcttttagt gcatgcattt gttccccata
    24361 aaggaaaatc atggcgaatt tctcaggatc gatatgttca ctcatttgcg catgcatatt
    24421 taaagactct ttccctaatt ccagcatatc tgctaacaca gcgattctgc gtccttttgt
    24481 tggcagattt ttaaaagtat ctaataccag tcccatggct gttggatttg cattgtagac
    24541 atcgctcaaa atagctgcac catttcctgc cgttagccat tcggttctgt tttgagtgat
    24601 agaaacatgg gcgagccctt ggcgtatctc aggtatggac agtccaaaga agcgcccgat
    24661 cgtatacgca atcaaagcat tcgttacatt ataacttcca ggcagcggga tcgtaaatgt
    24721 ttctccttct actacaaaat cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac
    24781 atcgccattt ccaataccga atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt
    24841 cagcaacggt tcatctgcgg gcacaacgag taatccgtct gatacaagtc cgtcagtgat
    24901 ttccattttg gctttagcga ttccttctcg cgacccaaga ttttctatgt gtgcttcacc
    24961 gatcattgtg attgctgcag cttttggttg agccataagt gaaagttccg tgatttcatt
    25021 ggcgtgatcc atgcccattt ccaaaacgat cttttctgta tcttggggca tgtgcaaaat
    25081 cgtgtaagga agaccaatat tattgttgta atttccttgc gtcttatatg ttttgtattt
    25141 ttcagacaag acagacgcgg ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat
    25201 tgcaacgact tctgcgccta ctttttccaa gtaataggtt gccaattgct gcatggcttt
    25261 caaaggatct gctacaggaa taactggaaa attttttgga gcttgttcaa caggcaagct
    25321 ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg ataaattcat gtccatctcg
    25381 cgtaccagca agaggaacga ataaacttcc ttcggttatc ttccggctgt caaattcgac
    25441 attggtaatc tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa tttcttgtat
    25501 tgttagattc attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg
    25561 gcgggttcgc atgactctca cttccaagta aatttctcta ttcgtattta gaagctagaa
    25621 tgaacgcggt tacgcaatca ctccagcctc ttctttattt ctctttattc atttttttca
    25681 aaaaaagatt gacgctgatg gtatcgattc attcctaact ggatcagttc ttcaatcaaa
    25741 tcaccgtatt tcaagcccat attttcccat aagagtgggt acatactgaa ctccgtaaat
    25801 cctggcatag agtttaattc attcaggaat aattcatttt tatttgtcaa aaagaaatcg
    25861 caccggctca atccgcttcc acctaacatc gtgtaagcta acttcgcgta ctcttgcgct
    25921 ttttgataaa cttcttctgg cacttcggct ggaatctgca tttcgatttt attattgata
    25981 tattttgctt cataatcata gaatgctacg tcttttacga cttcaccagg caaagtcgtc
    26041 cgaacgtctt catttcctaa tacagcaact tcgatttcgc gcgcttcaat tccttgttca
    26101 acgattgctc gagaatcata ctgataggct gttgctaaag cattttgcag ctcttctcgg
    26161 ttttctgctt ttgtaatgcc gacactagaa cccatattcg ccggtttgac aaacatcgga
    26221 taaagcaaag aaccttcaca ttgatcaaat acttttttag gattttcttt ccattgattc
    26281 ttaagtactg gtacataagg aacttgcggc acaccagcag cttgtaaaat atacttggtc
    26341 atgattttat ccatggcaca tgcactggtc aatacgcctg cgccgacata aggcatattc
    26401 aatgtctcta agaagccttg gatcgttcca tcttccccgt ttggcccatg taaaactgga
    26461 aaaacgatgg ctccttcttc tttgatttcg cccggattga tcacttttcc tgtaaagcct
    26521 tcctctgtct gtccacttgg gtcccatgaa agatgcaaga catctttgct ggcaggtttt
    26581 tctgttagta atggaccttt gacccattgt ccttcttttg taataaatac gagttgaact
    26641 tggtaataat tataataaat ggcatttaaa actgaaaatg cggaaagaat ggacacttca
    26701 tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc ctcctgtatt
    26761 ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa acaaaaataa acgaataact
    26821 tatttataaa aaaacgagcc agcttctaaa agaacgagcg tcatttattt tttgtcagct
    26881 agattatttg tcccgttttt tataaaaagt tatgataaag aaaaggaggg atgatgatgg
    26941 cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg agtgttcatc aattacaatg
    27001 tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac
    27061 taggccccac cttcatcaag ataggacaga tgctttctgt tcgagaagat ttgctttctt
    27121 ctgccttcac tcaaacattc aaaacattac aagatagtgt acctagcgac acattttcaa
    27181 cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt atcagatata ttcgatgatt
    27241 tttcaaaatc gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc aagctgaaga
    27301 acggtgacgc ggtagtcgtt aagatccaac accctaacat cgccgaagag atccgacttg
    27361 atcttcaact atttgaacga gcgatccctt tgatcaaata cattcctgaa actagcgtag
    27421 ttgatctaaa aggggtactc caagaagtaa aacgttcctt gatcaatgaa atggacttct
    27481 taaaagaaag tcaaaatggc gaacaattct atcaaaagaa caatggctgg aaagagatcc
    27541 gcagccccaa gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt
    27601 ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact tttatcactg
    27661 gaattcaaaa caagcagctg aaacaagaag ttgcaaagct tttagttgag aactttatga
    27721 aacaggtgtt tgatgatggt tttttccatg ctgatcctca tccagggaat ctgctattcc
    27781 atgtactaac aaaagaagaa cagacacaag ctagccgaaa aactgaaaca gtccatgaaa
    27841 aagagttcgg ttcctttgct tttcgagcct ccacttctgc agaagaccct gttgcgccat
    27901 ataccatcaa ctacatcgat tttggtatga tggggcatct ttctgcagga cttaggcaaa
    27961 aactcacaca agccgtttta gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg
    28021 tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac caagaactga
    28081 caagttttct tgaacagtat tatgattctc ctattgatga aatcaacttg caagaagtat
    28141 tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc
    28201 ttttgctgaa agcatttggg acattagaag gggtgatccg cgtcctcgat cctgaagttt
    28261 ccctcatgga agtcgcttcg ccttttgctc aacattattt tctcactcat ctggatgtag
    28321 aagatacatt gaaacaaagc ggattagact tattggaagg gatgaaggct gcgcctaaga
    28381 tccctcaaca gctccaccat ctattagaaa tgtggacttc gggacaagga aaagtcaatt
    28441 tggaattgaa aaagcaagac aaattattga gcaggatcga aagtatgatc aatcggcttg
    28501 ttttcggtat gattttagct gcattgatcg tagggtcctc cctgcttgtc caagcagcac
    28561 ctgtagaaaa cgcagaggtc gtttccttat tagggatatt tacgtatgct attgccgcat
    28621 tcgtcattat ttttttggca atcgatgcat tgatccaaat gtataagaag aggaaaaagt
    28681 agaaaaaggt tgtgacagaa agaatcagct tcaagaaata agaaggggtt ttacataaat
    28741 tgtttctcaa atttttgtga aaattcggct tatttttcga agaggctact tctgaaacac
    28801 cgagaataaa aaatgtgaaa aagctgttct gcacccagta ataagaacaa tcaaaaaggg
    28861 aacatggaac acatttatcc tgtttccctg ttcccttttt gatgtattcg agcataaata
    28921 aaataccttc gttacctact atcttctgta cttggtaaac cttagtcaat tgcctttttt
    28981 aacgcttgac gaaaagacat atgatctttc tccgtaaagg gttttggacc ctttgtcctt
    29041 atgccagctt ttcttaactt tccactatga taacgttgtt ctagaagcgt gccgatattc
    29101 gcttcgtcaa attcttttcc agagtgatgg aacactcgca cacccttcgg caatacaagt
    29161 gaagctaaac cataatcttg cgtgatcaaa aagtcattct tcttaaccat accgacgatt
    29221 ttataatccg cactatcagc tccgcggtct acgtagacga tttggacatg attagcgtat
    29281 tctttggtcg tataatggtc cacactggtc acgataacca cgtcaagacc ataggattct
    29341 cctaatttta tgacatcttc ttttactgga gaaccatctc catcaatgaa tagcctcatt
    29401 ctttttcgcc ttcttccata aatgataaac agttactaat ccgctcagta aaagaccaac
    29461 attgatccat tttgccaaga taagcagttg tacactttct atcggcactg attcatggtt
    29521 cagcgtgatc aataatagat ggattggctc tcccatttct tttgctaaaa caaacaaaag
    29581 cgtcatcaat aaaaactggt ggatcacact ccctttattt tgagtagtat acttgacaac
    29641 gatccggcga tccattggaa tagctgtatc agtttgataa tattccttat ttagccagct
    29701 gcctgttact acgtgccaga aactaagcag aaagacacct atcgcaaaac catttggaat
    29761 caagaggacc aataaagaag aactcagtcc aagcaattgc atagccaatg gagaccatgt
    29821 taaacgtttc aaaatccatg gtccgactat ttttgctgca atcaagcctg ctgcataagg
    29881 caaatacata aaaatcgaaa gtttttctaa tccgtacagt atgccaacat aaaaactgct
    29941 aaataaaaaa aggaaattcc ctagcattcc atttagaaaa gtaaacagat ttagccagct
    30001 tggcaacttc cattccttct tctggtgtcc taaaacaaac acgaacaaga tgaacagcag
    30061 caagaaacca taaatagcaa gatcaaaaag cctttcatcg aataacaatc gggcatttct
    30121 caaaagaaac aataagccaa agaatatcaa aaataaaaca agctctcgcg tagctaccag
    30181 atttcttttg atgtctttga aatccagttc gtaacgcgga taatgactaa ccgtatggta
    30241 ggccatgacg taaaaaagtg tataaatagt aaagcttgct gcaatctgag tcggtgcagg
    30301 caactcaatc gcttgataca ggagaaatag ccagatagct gcaaataagt actttttccc
    30361 atccatattg ataaaacctt gcgtcttttc gtgaaaattg accgtggtat tagcaggtac
    30421 aagccatgct gcactcagcc ctagcaaaac acttgaaaaa taaaataaag gaaaataaaa
    30481 tacgcctaat attccaagca atgaaccagc accaccaatc agcagagcga tcatcagcaa
    30541 cgtgtaacta tctagaccaa atcgtagact gttgactaaa aatagtccag tcatccgaaa
    30601 ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg ttagtcccaa
    30661 aaataaaaga taaggaataa aaataaccgt gttacttaag ataaagggcg ttgtttgtgt
    30721 aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca atggctcctt aaacagtctt
    30781 ataactaatc tgctcttcga ttttagcatt aaaggggaaa taaaccaatt ttacagaata
    30841 tattcttgta aaaaaaaata cagaaagaag cataagcttc tttctgcatt tttacggtac
    30901 ttgattactc aagcagacaa gcccttctcc acaaaagtct tgcccgacac ccctgcatcc
    30961 ggcaggtgat aaagatagaa atcaattatc ctgtgccccc aaaatcaatt aggagctctg
    31021 taaaccagag tccttctcga cccgtcgcat aaatttactt taacacagtt cctatgttta
    31081 tgcgagagct tttttcattt tataatcgac catttgaaat ccttgaccga tatctgaaat
    31141 aagttcttct gtaatctcaa atccttcatg cttataaacc tcgattgctt gatgattccc
    31201 ctgattcact cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt cttcatacca
    31261 gcgaaaaact gaagaagtca atccttgacc acgtacttta tttagcaaat ataatttact
    31321 taaataaatt tttccattta tctcttcata agcagtgtaa ccgacagtct tttcttcgct
    31381 ttttaataaa aagtaggaca caccttctgt cagttctttt tgaatctgtt tttttgactg
    31441 ataagttttc agcatgtaat ccacttgttt ttgaccaatg atcggcacaa aaacttccgg
    31501 ccatacttct tgtatgactg ccatcagctc atccagttca ttttctgtta cttttatttt
    31561 ttccatacac ccacgtcctt ttttacatta taaatgacct tgcgcttgat ggaataaaaa
    31621 gagcatgatc atgctataca cacccaccga ccacggtttc aagaaaatca caatctgcat
    31681 aaattcccta aaagataatt tcgtcaaacc agctactaga cagatcaagt cgtctggcgc
    31741 aaagggaagc agcaaggtaa caactagtac ttttttaata tctttcgttt ttcttgtcag
    31801 caattcatca aatttctgat acttttttgg cgataaaata agctggacaa aggctacgcc
    31861 atagtaacgg acaagcaaaa atccgattgc ttccccaatc actaggccaa tacaactata
    31921 taaaagccca aatccattgc caaacatcag catgcccgct actgaggaga tccctcccgg
    31981 caaaatagga atgatcactt ggatcacttg caacagaaca aatatcatca ctgcatactt
    32041 gccaaattgc tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata
    32101 agcataaaga atcaacacaa agaaaaaaaa cagaccaata atgggcatga tctgaagaca
    32161 tttttgtaaa actgatcctt ttttagacat ctatattccc cacatctttt ttcatttatt
    32221 aaaactgcct cct
//
LOCUS       DAYYFD010000027        32216 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000027 DAYYFD010000000
VERSION     DAYYFD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.027"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..1026)
                     /locus_tag="ACHA25_001118"
     CDS             complement(151..1026)
                     /locus_tag="ACHA25_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8762423.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1135..2904)
                     /gene="aspS"
                     /locus_tag="ACHA25_001119"
     CDS             complement(1135..2904)
                     /gene="aspS"
                     /locus_tag="ACHA25_001119"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8762424.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2919..4211)
                     /gene="hisS"
                     /locus_tag="ACHA25_001120"
     CDS             complement(2919..4211)
                     /gene="hisS"
                     /locus_tag="ACHA25_001120"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8762425.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4605..5552
                     /locus_tag="ACHA25_001121"
     CDS             4605..5552
                     /locus_tag="ACHA25_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8762426.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5641..6900)
                     /locus_tag="ACHA25_001122"
     CDS             complement(5641..6900)
                     /locus_tag="ACHA25_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8762427.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7071..7517)
                     /gene="dtd"
                     /locus_tag="ACHA25_001123"
     CDS             complement(7071..7517)
                     /gene="dtd"
                     /locus_tag="ACHA25_001123"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8762428.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7528..9741)
                     /locus_tag="ACHA25_001124"
     CDS             complement(7528..9741)
                     /locus_tag="ACHA25_001124"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8762429.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9974..10738)
                     /locus_tag="ACHA25_001125"
     CDS             complement(9974..10738)
                     /locus_tag="ACHA25_001125"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8762430.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10739..11686)
                     /gene="prmA"
                     /locus_tag="ACHA25_001126"
     CDS             complement(10739..11686)
                     /gene="prmA"
                     /locus_tag="ACHA25_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8762431.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11699..12187)
                     /locus_tag="ACHA25_001127"
     CDS             complement(11699..12187)
                     /locus_tag="ACHA25_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8762432.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12153..12824)
                     /locus_tag="ACHA25_001128"
     CDS             complement(12153..12824)
                     /locus_tag="ACHA25_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8762433.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12962..14248
                     /locus_tag="ACHA25_001129"
     CDS             12962..14248
                     /locus_tag="ACHA25_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8762434.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14320..14514
                     /gene="ssrS"
                     /locus_tag="ACHA25_001130"
     ncRNA           14320..14514
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA25_001130"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14592..14876
                     /locus_tag="ACHA25_001131"
     CDS             14592..14876
                     /locus_tag="ACHA25_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762435.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14949..15428
                     /locus_tag="ACHA25_001132"
     CDS             14949..15428
                     /locus_tag="ACHA25_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8762436.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15651..15794
                     /locus_tag="ACHA25_001133"
     CDS             15651..15794
                     /locus_tag="ACHA25_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8762437.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16139..16267
                     /locus_tag="ACHA25_001134"
     CDS             16139..16267
                     /locus_tag="ACHA25_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762438.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            16372..17955
                     /gene="abc-f"
                     /locus_tag="ACHA25_001135"
     CDS             16372..17955
                     /gene="abc-f"
                     /locus_tag="ACHA25_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8762439.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLAKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18392..19576)
                     /locus_tag="ACHA25_001136"
     CDS             complement(18392..19576)
                     /locus_tag="ACHA25_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8762440.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19601..20608)
                     /locus_tag="ACHA25_001137"
     CDS             complement(19601..20608)
                     /locus_tag="ACHA25_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8762441.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20780..21139)
                     /gene="comGG"
                     /locus_tag="ACHA25_001138"
     CDS             complement(20780..21139)
                     /gene="comGG"
                     /locus_tag="ACHA25_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8762442.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21111..21536)
                     /gene="comGF"
                     /locus_tag="ACHA25_001139"
     CDS             complement(21111..21536)
                     /gene="comGF"
                     /locus_tag="ACHA25_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8762443.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21499..21846)
                     /locus_tag="ACHA25_001140"
     CDS             complement(21499..21846)
                     /locus_tag="ACHA25_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762444.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(21818..22288)
                     /gene="comGD"
                     /locus_tag="ACHA25_001141"
     CDS             complement(21818..22288)
                     /gene="comGD"
                     /locus_tag="ACHA25_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8762445.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22263..22541)
                     /gene="comGC"
                     /locus_tag="ACHA25_001142"
     CDS             complement(22263..22541)
                     /gene="comGC"
                     /locus_tag="ACHA25_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8762446.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22538..23587)
                     /gene="comGB"
                     /locus_tag="ACHA25_001143"
     CDS             complement(22538..23587)
                     /gene="comGB"
                     /locus_tag="ACHA25_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8762447.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23544..24473)
                     /gene="comGA"
                     /locus_tag="ACHA25_001144"
     CDS             complement(23544..24473)
                     /gene="comGA"
                     /locus_tag="ACHA25_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8762448.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24605..26359
                     /gene="ade"
                     /locus_tag="ACHA25_001145"
     CDS             24605..26359
                     /gene="ade"
                     /locus_tag="ACHA25_001145"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8762449.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26457..27119)
                     /gene="trmB"
                     /locus_tag="ACHA25_001146"
     CDS             complement(26457..27119)
                     /gene="trmB"
                     /locus_tag="ACHA25_001146"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8762450.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27189..27971)
                     /locus_tag="ACHA25_001147"
     CDS             complement(27189..27971)
                     /locus_tag="ACHA25_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8762451.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28175..29386)
                     /locus_tag="ACHA25_001148"
     CDS             complement(28175..29386)
                     /locus_tag="ACHA25_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762452.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29379..30116)
                     /locus_tag="ACHA25_001149"
     CDS             complement(29379..30116)
                     /locus_tag="ACHA25_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762453.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30254..30688
                     /locus_tag="ACHA25_001150"
     CDS             30254..30688
                     /locus_tag="ACHA25_001150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8762454.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30681..31025
                     /locus_tag="ACHA25_001151"
     CDS             30681..31025
                     /locus_tag="ACHA25_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8762455.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31205..32215
                     /locus_tag="ACHA25_001152"
     CDS             31205..32215
                     /locus_tag="ACHA25_001152"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8762456.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         9641 a         6748 c         5743 g        10084 t
ORIGIN      
        1 taaaaaaata aacatgcttt ttttcaactt taagaaaatt tcaaataaaa aagcagtttt
       61 tctttgctga ttctactatg gtagaaaaaa actgcagaca aacgaacgtc tgtctacagc
      121 agtatttcat gaatttttgt caaactcgat ctaaatgcca tcctcataga aacgaccaag
      181 tatctggacg ttttctgcaa tacttacaaa agcttgtgga tcagaagctt tcattgcttc
      241 tctaagtaga ggcagttcgt atcgtgtaac gatcgtcatc aatactgtct gcttatcatg
      301 cttataagca ccttctgctt cattgatgat cgtgatccct ctacgcatac agttttggat
      361 ttcatttact actttttctg gatgtttcgt tacgatcatt acctgcatct tcttttgttt
      421 agtataaacg acatcagtta cttttccact gacgaaaata gacaacgtac tgtaaaacat
      481 atattgccag ccaaatagaa ttccggcaac aaaaacgatc aatgcattga agtagatcga
      541 aatagaacca atcgactttc ctgttttttt acgaatcgtg atactcacga tatctagacc
      601 gcccgatgat aggccgtttc tcaacgctag cccaatacct agtcccatca cagccccccc
      661 aaacaacgca cagatgattg gatcggtaga taggactgtt tccggtaaaa tatggataca
      721 aagggaggtc aaagtgactg tgataaaagt gaaaattgta aacttcttcc cgatttttaa
      781 ccacgccaat acaaataaag gtatattcag taaatacaaa gttgttgata ccggtacttc
      841 gaatcctatc agtgattttg acaaagtcgt caaaatctga gctagaccag taataccact
      901 tgaataaata tgtcctggct gataaaagaa attcaatgcg actgatgcga tcaacgcata
      961 aacgatagat accgaaagct tggtcgtgta atcatgataa gcccaatttt cgaatgtttt
     1021 tttcatggaa aagccccgct ttatatgtta tggcttcatt ataaagtgtt tttcaattaa
     1081 atcaaggttg tcaaaagaag cgtattcgac aaatcctttt taccttttta tcggctattc
     1141 ttctatattc gttacatcaa tattcaattc gaacaattgt aaaggcgaaa ccatacttgg
     1201 tgcatcactc atcacatctg ctgcttttcc gtttttagga aaggcaatga cttcacgaat
     1261 attatcttct ccggctaata acattgcaaa acggtccaat cctaatgcaa taccgccatg
     1321 tggagggaat ccgtaatcta aagctgttaa caagaaacca aattgttctt ccatttgttc
     1381 ttttgtaaag cctagagttt ctagtacttt ttcttgaagc tcacgagtat gaatacggat
     1441 agaacctccg cctagttcat aaccgttcaa gacgatatca taagcttctg catagacttt
     1501 tgcagggtct tcagccaata acgggatatc ttcttctttt ggcatcgtga atggatggtg
     1561 agcagagaca tatcgacctt cttcttctga aaattcaaat tgtggccaat cagttaccca
     1621 taagaaattg aatttcgatt catcaattag tcctaattct tttcctaaac gtgtccggat
     1681 agcacctaat gcagcagcga cgatttcact tttatctgcg ccaaacatca gcaagtcacc
     1741 tggttttgca tctgttgctt taatgattgc ttctgttgct tctcccatga atttagcaat
     1801 tggacctttc aatccgtctt cttctacttt cagccatgca agccccttag ctccgaattg
     1861 tcctacatat tgacctaact gatccatgtc ttttcgtgag tagcgatctg cagcgccttt
     1921 agcattcaat gctttcacga caccgccgtt ttcaagagcc atttggaaga ctttgaattc
     1981 tacttctttg actgtatcgc ttagatcgat caattccata tcaaaacgtg tatccggttt
     2041 gtcgctgccg tagcgagcca ttgcttcatc ataggtcata cgaggaaatg gcaatgtgac
     2101 ttcgataccg cgaacttctt tcattacctt cgcaatcaat ccctctgtgt aagtttggat
     2161 ttcttctgct gttaagaacg ttgtttcaat atctacttgc gtaaattcag gctgacggtc
     2221 tccacgtaaa tcttcatcgc ggaaacaacg aacgatttga taataacggt cgaatccagc
     2281 attcatcagc aattgtttaa acaattgagg agattgcggc aacgcataga aatgtccagc
     2341 atggacacgt gaaggaacta ggtagtctct tgcgccttct ggtgtagatt tccctaggta
     2401 aggtgtttcg atatcgagaa agtcattatc atctaaataa tgacgaatcg cttttgttgt
     2461 cgcatttctt aatttgatgt ttttagtcat ttttggacga cgcagatcca aataacgata
     2521 tttcaaacgt atttcgtcac tgattgcttg atcatcttca atttgaaatg gcggggtttt
     2581 tgcttgattc aaaatcacga tttcacttgc aatcacttcg aattcaccag tggccatttt
     2641 aggatttact gcattttctc cgcgtcttct aacttcccca gtcacttcga tcacgtattc
     2701 actgcgacat ttatccgcga tttcccatgc ttctttgttg atttcaggat taaagacgat
     2761 ttgaacgatt ccttcacgat cacgtaggtc gataaagatt actcccccaa ggtctcgtct
     2821 tttttgtacc catcctttta atgtaactgt ttgttctaat tgttgttcgg aaactaatcc
     2881 gcaatagatc gttctttgtg ccatttattt ttttcctcct actcgaataa agtcatttca
     2941 tcatagactt catcaaaatg ttcatatatc tcttctaatg gaaaactttt ttcttttcgg
     3001 cttgccattg ctttgacatt aatcgtttga ctagcaagct catcttcacc gatcaccaat
     3061 actaatttgg cattcgcttt atctgctgtc ttaaattgcg cttttgcttt acggttcatg
     3121 aagtcgcggt cagctgaaaa accaaagttt cgaatcgctt ggaccacttt caatgcttcg
     3181 atatttgttt cgtctccgag tgcgaccacg taagcatcca actcattaaa tgcagggata
     3241 actacttcct ctgcctccat agtcaagagt aatcgctcga tacccattgc aaatccgaag
     3301 ccaggtgttg aaggaccacc tagttcttcc actagattgt cataacgtcc gcctgcgcaa
     3361 atcgttgctt gtgcccccat ttttggtgcg tcactcatga tttcaaaaat cgtatgtgtg
     3421 taataatcta gtccgcgaac catattactg tcgatttcat aaggaacagc caatgcatcc
     3481 agcatggaaa caacggtatc aaaatgtttt tgagcaggct cgcttagata atctaagata
     3541 gatggagctt ctgccacaaa tttttgatct cgtttgtctt tactgtccaa tacgcgtaat
     3601 ggattttcat gaaggcggcg tttagagtct tcactcagct ctgattcaaa aggtaaaaga
     3661 tagtcgatca atgcttgacg ataggcttga cgcgtttctt tatcacctaa tgaattgatg
     3721 actagccgga tttgcgtgat ccccaactgt ttgaagaaat caagtgccat caccatgctc
     3781 tccacatcag ttgcagggtt ttctgatcca aatgcttcta ctccgatttg atggaattga
     3841 cgtaaccgtc ctttttgagg gcgttcgtag cgaaacattg gtcccatata aaaagttttg
     3901 aacggtttcg catactctgg gccgaacaat ttatgctcaa caaaagaacg aacgattggt
     3961 gctgtacctt ctggtcgaag cgtcacatgg cgttcccctt tgtcataaaa atcatacatc
     4021 tcttttgaaa cgatatccgt tgtatcccct acacttcggg atatcacttc atagtgttca
     4081 aaaatcggtg tacgcatttc attatactga taatctctga ataataaacg tgctgtttct
     4141 tcgacaaact gccatttttc tgattcgcct ggcaaaatat catttgttcc ttttggtctt
     4201 tggaaactca tagttttctc tccctttctt agctatgtaa ataaaaaaat cacccttgtt
     4261 catcatgaac aagggtgaat gaaattcata cacggtacca cctaattttg aaagatttct
     4321 ctttcctctt acgaataacg ctcgtacgcg ggacggcttt tcactcgtcc atctccagag
     4381 tgtctttagg ctaatgaaac tttagatcat tttcagcctc gatgatcctc tcttgtaaag
     4441 tttcaaaaac ttactcgctc tttcattgac attcgatatt acttataacc tactaaaaac
     4501 cagtctcaaa gtcaagagat atctgactat ttcccctcac aaaaacgttt tagttcagct
     4561 ttttggtatt ttgttttaga ctagacaaaa aggaggaatc agctatggca gaaataagaa
     4621 tcggacattc acaagtctac gcagaacagc ttggattagg agcaaatgcc gttggaggac
     4681 ataatctttt tgacggtttg gaagatgaga caggaaaaca agtagttcgt acagctttga
     4741 atagcgggat caatttgatc gataccgctt atgcttacgg aaacggccgt tccgaagaac
     4801 tgattggcga ggtactgaag gaaaaagaat atgatcgctc acgtgtcgtc atcgctacaa
     4861 aagctgctca tgtaccaaat aaagggcgaa catttgataa ttctcctgag tttctcaaac
     4921 aatcggtgga agatgcacta aaacgtctgc aaactgatta catcgacatt ttttatatcc
     4981 actttccgga tgaaagcaca ccaaaaaatg aatcagttgc tacattgcac gaattaaaag
     5041 aagcaggaaa aatccgagca gtcggtgtgt caaacttcac gttagaacaa ttgaaagaag
     5101 cgaatgcaga tggatatgtc gatgtagtag aagataaata cagtttgatc catcggcagg
     5161 ctgaaaaaga attattccct tatcttgaaa aaaacaaaat cagttttgtt ccctattttc
     5221 ctcttgcttc cggtttgctg acaggaaaat atgaacttgg tgaggaaaaa caatttggtg
     5281 aaggagatcc tcgaaaaaga aatccagatt tccaaggaga acgtttcaga gaaattttaa
     5341 ccgctgtcga tgtacttcgc cccattgcta aaagatacca agccactcct gcgcaacttg
     5401 ttttagcttg gtacatgaag aatccacgag tgtctgtagt tatacctgga gctaagcgtc
     5461 cagagcaagt atctgacaat gtccaagctt tagatttgca tttatccaat gaagattatc
     5521 aaaccattga tgaagcattt cgtggatttt gatcaattga ccttatcaat ttatagtaat
     5581 atgaaaaaag gaagatcagc actgtttgat cttccttttt ttcgataagt ttatgctaat
     5641 ctaattcttg taaagtagcg ctcggctatt cggataagtc ggaaactgat tgaatggaat
     5701 cgtccaatct tgttcaggta cagagaactc aaacttattt actagctgat cggaaaacac
     5761 acgtagacta tgaagagtga tccattctcc tgcacagcga tgcatttgtc gaaaatttcc
     5821 cccgccttgt gcaatcatct catattcttc tttataggaa atatcttttg ctttccctac
     5881 ataccgtttg atcataaagg aatctggcgc ttcaactgtt cgttcatcat ggtctgttcc
     5941 atataaatct aaaataaccc aactgccttc tggtatacga taaccatcta cttctacttc
     6001 tttcagcgaa atcgcaggta gcattgggaa gaaaggataa tagcgtctca tttcttgaat
     6061 aaacggatct tgtaacgtcg aaaagtcatt ttttaattgc tgatacaaat ctggtctact
     6121 gaaaagtgca tgtcccatca atgccgccca cacagttaga gcaaccgttg gacggatgat
     6181 gttcaataat tcaacagctg ccacttccag aggtaaaagc tgaccatcta aatctgtggc
     6241 attggcaaaa gcatataatg ccacattttc ttttccagcc acaggatttt gtcgagcttc
     6301 ttcaatcaag ccttgcgccc atttttctga ttttttgcga ttttcgacac cttttatgtg
     6361 atcaatcgga gaagtaaacg ttccgctgat catagagatt tgatattctg ctaatttttc
     6421 gacctcatct ttagaaatcg ctgataaatt gatacctgcc cactcgcaaa tagatgtaaa
     6481 gaaaaccatc ttacttaaat caaatagttc aaattgacca tgctgtgctt caagtgcttg
     6541 tgtcagattc ttatctaaga tacgatgata atcttccatt ctttccggtg tcattagatc
     6601 cataaaaata tttttccgat ggtgatgagc agctccatcc aaagtctgta ctccaccttg
     6661 accaaaaagt gtcttcaata ctaattttgg catagcacct tttcgtttga aattccttgg
     6721 atcataaaat acttttgctg ctgatgatcc gtagatcgtg atagcttctt tattgaaaat
     6781 ctttgctttg acgacgggag cgtccgcttc atggcgtaac tcctccaaca tgttgtatcc
     6841 tttttgatac aattttttta aatctgtgat tttgatatct actactggta cttctttcat
     6901 cttcagcaac ctcctataaa aaataacttg tataatttaa gcttaacttt ttttccgact
     6961 gctgacaaaa gaagcgcttt ttgcagacaa aaaagccact taagttagag tttatcagtc
     7021 taacttaagc gacgtgtttc atagttttta ctagtgaaaa ccagattact ttatttgttt
     7081 ttagaatcaa taatgatcgt tacaggtcca tcattgacga gagaaaccga catatctgct
     7141 ccgaattgtc cggtagcgac tgtgatgccg cgacttctga ttccttcatt gaatgattcg
     7201 tataaaggaa tagctgtttc tggtcgtgcg gcttccacaa agcttggtcg atttcctttt
     7261 ttcgtatccg caaacagtgt gaattgagag atacttaaaa tttgtcctcc cacatcttct
     7321 acactaatat tcatttttcc ttgttcatct tcaaaaatac gcatttgtgc aatttttttt
     7381 accagatagt ccacatcttt ttgcgtatcg gtttcatgaa tgcctaataa aacaagtagg
     7441 ccgtagccaa tttttccaat ttcttgctga tcgactgaca cagcagcttt acttacccgt
     7501 tgaacaactg ctctcatatc tgtcctccta tccattcgtc cggcgtacac tgtagacatc
     7561 aggtactgct ttgattttat ccacgatact ctttaggtgg gataaattct ggataccaac
     7621 cgtcaaatgg atcgtcgcca ttttatcttt attcgtccgt gcttcaacgc tgttgagatt
     7681 tttcgttagc gcattgactg tttgaagtac atcattcaat aagcctgaac gattatagcc
     7741 gtaaatttcc aaatctgcat catactcttt tcgtgtattc gatgtatctt cccattcgac
     7801 ttcaatcaaa cgttctgcta catttggttt gtcaggctga acattcggac aatctcgacg
     7861 atgaatggat atccctctgc ctttagtgat ataaccaacg atatcatcac caggaatcgg
     7921 attacagcag cgactgatac gaattagtaa gttttctacc ccttgaatga caacgccacc
     7981 ttcatgacgt actttcattt tgtcaggttc ttttttcggc tgattcatga tttcttctgc
     8041 ttcttgcttt tgctgctcga ttttttgttc tttacgttct ttttcagtca gacgattggc
     8101 catcgtcaag gggctaactt ctccataccc tacagctgca tagagatcat cttctgtttg
     8161 ataattaaaa cgatcgagtg cttcttgcag tttgttcttc gtcaaaatat ctttaggcgt
     8221 aaatcctaga tctgtaatac atttgaccac ggattcgtgg cctttgatca cattttcttc
     8281 tcgatcttgg gctttgaaga aacgtttgat cttatttctt gctttgctag tagcaactaa
     8341 tttcaaccag tcgcgacttg ggccaaatga atttggagaa gtcatgatct caatgatatc
     8401 tccgtttttc aatttgtaat caagctgcac cattttgcca tttacttttg caccagtggt
     8461 tttgttaccg atatctgtat ggatactgta tgcaaagtcc aatggtccgg atccttttgg
     8521 taactcagta acgtctcctt ttggtgtgaa gacgtagact ttatcactaa agatatctcc
     8581 ttttacgcct tccataaatt cagaagcatc atagctttcg tcttgaagtt cgagtatctc
     8641 atggaaccag cttaattgtt tcgtcatacc atctggttct actttttcat tttttccttc
     8701 tttataggcc cagtgtgcag caaccccgaa ttcagcgatt tcatgcattt cctgtgttcg
     8761 gatttgaatt tctaccggat ttcctgccgg accgatcacg gtagtatgca aagattggta
     8821 catattagct tttggcatgg caatataatc cttaaatctg ccgggcattg gtttccattt
     8881 tgtgtggatt gctcctaata cagcataaca atccttgatt gaatctacga tcactcgaat
     8941 agctagcaag tcataaattt cgttgaactg ctttttctga tctttcattt tacgatagat
     9001 tgaataaatg tgttttggcc gtccatagat ttctgcaaaa atccccagct cttctgtcgc
     9061 tattcgaata tcttcaacag taccacttac gtatttttca cgttcttctc tcttcgtctg
     9121 catcaagtgg acgatacggt aatactgttt tggatttaga taacgaagtg ctgtatcttc
     9181 taattcccat ttgatccggc tgatccctag acgatgtgca agaggcgcat agatttctaa
     9241 agtttcctga gcgatccttc tttgcttatc ttctcgcagg tgcttcaacg tacgcatgtt
     9301 atgtagtcta tcagctaatt tgaccatgat aactcgtaaa tcttgagcca ttgctagcag
     9361 catctttcgg tggttttctg ctagctgctc ttcgtgggat ttatatttta ttttccctaa
     9421 tttggttacg ccgtccacta acatagcaac gtcatcgcca aattcttctc tcagatcttc
     9481 taaagtgatt tctgtatctt caacaacatc atgtaagaag cctgtagcca ctgtatgggg
     9541 atccattttt aaatctgcta atatacctgc gacttggata ggatgaataa tgtagggttc
     9601 gcctgatttt ctaaactgtc cgtcatgtgc agcagtcgcg tattcgcatg ctttctcaac
     9661 aaatgctaca tgttcgggac ccatgtattg actaacgagc tttatgacac cgggtcccgt
     9721 taaaatttct tcttttgtca caggaatcga tccccctttc ttttttcggt aaaacgggaa
     9781 tcgggacaaa tatctgtttg ataattgtcc caaaatccgt gcagttatac gtttttttac
     9841 ggaaatgttc gctttttaaa agctttttca aacgattttt cgtatccatt gtataaataa
     9901 ttgtttctat tatacaaatt gttgactatt ttttcaattc tttctgtcga tactctttta
     9961 ttaatgaaga acgttagctg aaagaaggat tcattaattc ccattgatag ctagcagcac
    10021 ttaataaata aagaggtgct gtttctgtac gaaggattct tggtcctaat ccacaaagta
    10081 tccctttgtg ttctacaaaa taatcgactt catcgcggga taatccacct tctggtccaa
    10141 agacagctaa gattcgacta tctggttttg tttcactaag aattttggct gcatttgatt
    10201 tttcgccttg ctttgctgat tcctcataag caaccaagac cacgtcataa tcatctagcc
    10261 gctcaaataa atcttgtttc gtttccaata actctactga tggcacgatc tgccgatggg
    10321 attgttccgc tgcctcttga gcaatctttt caagccgttg ttgtcgtttc atacgttttt
    10381 ttgcatccca tttaacgaca gaggctgatg ccgggaaacc aagaaaagct tgcgctccta
    10441 attcagtgga tttctgaacg atccaatcca gcttttcccc tttaggatag ccactcgcta
    10501 tggtaatcga acttggcagt tccttttgat tttcttcttt tttaatttct ttcagataaa
    10561 cagcctcttc agtaatctcc gtgatttctg caacaatggc tatttgatca ttgaatgcca
    10621 aaaaaacttc atcctcgggt tccatcctca taacccgtac aatgtggtga tagttttccc
    10681 cttctaaaac aaaatagtct tttgattcat atgattcttt taaaaaatac cgctgcatct
    10741 attccacctc tggttttttc aaaataattg cataccagtc tttttgctgg aaaatttgat
    10801 ccacgataaa cccttgttct aacatggcat ccagaaccat ttgcttcttc tcttcaatga
    10861 ttcctgaaac gatcagcgtg cctgtttgct tcagtaaacg ccaagcatct gggatcatca
    10921 ataagatgat atctgctaaa atgttcgcaa cgattacatc actttcaatc tcgattcctg
    10981 tcaaaagatc gttagctgat acatggacat ctttggctat ttcattcata tccatatttt
    11041 ctttcgccga acgaacggct acttcgtcta aatcaaaggc atagacatct tttgcaccaa
    11101 gatattttgc tgcaatactt agcacgcccg acccagttcc cacatctagc aatgtttcac
    11161 caccacgcaa gactgtttct agtgctgtca atgtcaagga agtcgtggga tgagtgcctg
    11221 tgccaaatgc cataccagga tctagtgtga taattttttc tagaggatct tctgtctggt
    11281 agttttccca gcttggcgca accgtcagca ttcgcgtaat acgcaccgga tgatagtatt
    11341 tcttccaggc tgttgcccag ttgctttcct ctacttcact aactgttact ttattttttc
    11401 caatagctag accaaactca ggtaattgtg tgatacgatc tttgataaaa ggcaagattt
    11461 ccggtaagaa agtcgtttcc ggaaaataag ccatgactag tgcgccttct ttcagcgatg
    11521 taaaatcatt tttatctaaa atctcaccat attgatccga ctgaaaatgt tcaacatcta
    11581 aagaatcttc aatcgcgaca ccactcgccc ctgcctccat caaaatatta gcaactgctt
    11641 cgactgcttc actttcagtt tctactttta cttcattcca tttcatatct gtcactcctt
    11701 aataacttgg gtacggtaaa taagtatctg cttcattttg ctgttttact tctactaatt
    11761 ctgctagttg ctgattatcc cagtgaagct cgaatacagc ttcgtcatcg tcttcatcca
    11821 aaaaatcaag caaatcgctt tgcccttgag ctaatacttc atttagatat tccactaaag
    11881 aatcagctac tgattttgcc aacccttttt ttccttcata agggattact gctagataat
    11941 tgcgttcatc aaaaacagat ttctgaggat taaaaagaag tacgccgtct tcaaattcga
    12001 taattggttc ttgcgaaacg atcccttctg catcatcaat ctcaaattgt gctttatttt
    12061 ctgcaaacaa gcgaacaacg atctcgatcg tatgattttt tgtatcccaa tctaaagcta
    12121 catcatagtc tgtcacttgt ttttcaatta tttcatctaa atatgttaat agatccttct
    12181 ttttcatttt ctttcctcca accccaatct ttctcgattt ggttttttct ttgtaaagag
    12241 atataaggat ttcccgacac aacaaaacgc aacggctttt ctgtccatac acctttgttt
    12301 gggataccga ttctaggcaa agcattgatc ttttttggtg tctttttttt ctcaaaaact
    12361 aagcgtaacg aactatctgc tatagactga ccataaagtt cttgaggaat tgctaatgct
    12421 tccactaatt ttcccggacc gttgctgata tctggaccag tttttccgcc acgattttta
    12481 ctcatttgat caatcattgc ggcgggttcg attgcacgta tcattactcc ttgaggaatc
    12541 ccttctggct gagtaatgat gttcaaaatc cgatgagtat gcatcgtgta aaggtagatc
    12601 gtacctggtt tttcatacat tgcccgcaca cgcggtgttc ttcttaaacc atagctatgg
    12661 gcggcctcgt cttctggacc taggtatgct tccgcgtcta caatatatcc agctaatctt
    12721 ccttctggtg tgtcatgctc caaatacatc cccaataaat cccgtgccac ttctggcgtc
    12781 gttttattcc aaaaaaattc atctatcttt tgtttatccg tcatttttct cacctacttt
    12841 atcatacaca atattagttg gataagctat gaaaacaaaa caacagaagg ctgttctttc
    12901 taggcatctt gctaaatatg atataattaa gggacgtaaa aacaagaagg aggaaaatcc
    12961 tatgcaacag ccattggctt atcgcatgag acctcgaacg atcgatgaag tcgttggtca
    13021 gcagcatcta gtcggggaag gaaaaatcat acgccgcatg gtcgatgctc gcatgctctc
    13081 ttccatgatt ctatatggac ctcctggtac tggaaaaacg agtatcgcaa gtgcgatcgc
    13141 cggctctact aattatgcat ttcgcatgct aaacgccgct acagattcaa aaaaggatct
    13201 acaagttgta gcggaagaag caaaaatgag tggtacagtg atcctcttat tggatgaagt
    13261 acaccgatta gataaaacaa aacaggattt tctgcttcct catttagaaa gtggccgaat
    13321 catcttgatc ggagcgacta ctgagaatcc ttatattacc atcaatccag cgatccgcag
    13381 ccgtacacag atctttgaag tcaaaccatt aacagaacaa gacatccagt tggcagtcga
    13441 acatgcttta agggataaag aacgcggcct tggacaacaa gcaatccagt tagacgaaga
    13501 tgctcttctt cacctttcgc gtgcaacgaa cggcgatttg cggagtgcat tgaatgggct
    13561 ggaactggct acactatcga ctccttcaga taaagaagga aggattcatc ttacactgtc
    13621 gatcattgaa gaatgtgtcc aaagaaaagc tcttacccat gacaaaaatg gagatgctca
    13681 ttatgacgtt atttcggctt ttcaaaaatc gattcgtgga agtgatgtgg atgctgcact
    13741 tcattatctc gcccgtttag ttgaggcagg tgatctggca agtatttgcc ggcgtctgat
    13801 ggttatcggt tatgaagata taggtttagg taatcctgct gctgcggctc gtacagtgaa
    13861 cgctgtatta gctgctgaaa aattgggatt gcctgaggca cgtatcccac tagcagacgt
    13921 cgtcgtcgat ctttgcttgt cgccaaaatc caattctgcc tacatggcat tagatgcagc
    13981 acttgctgat attcgagagg gcaaagctgg agatgttcct gatcatttga gagatagcca
    14041 ttacaaaggt gcgaaatcac tcaaccgtgg cgtgggttat cagtatcctc atcattttga
    14101 tcaggcttgg gtcaatcagc aatatttacc agataaactg aagaatgctc agtattatca
    14161 gccaaaagat acagggaaat atgaacaggc tctgggtcaa cagtattacc gtataaaaga
    14221 gtggaaaaag catcctccaa aaaactagac aagaaaacgg ttctgtttct gcgagctctc
    14281 tcattgctct ttttaaattc atatgctact atagaggtgg gaaatctgtg atgttcgaat
    14341 tgtcctcttt gtgtgttgac cgaacatttt tattgatatc ttgggacccg tcttgtgtca
    14401 ctgctggtaa catgccttat catttggtag ttcgaagcta cgaccatggg acccacctgc
    14461 ttgaattcgc gggatcaata ctacaggaca aacaacggca ttaacggaga ttaccatctt
    14521 ttaaagaata aagcaatttg ctcttggcaa attgcttttt ctatttgttg cttattttgg
    14581 aggatttgtt catgattagg attttaatga ttatcgccac tttagtatta ttattcgtta
    14641 gctattattt atttaataaa caggatatct tttttgtatt gatcaacaaa aatgataaaa
    14701 atcaaggatt cctacaattt tacggagctg catatgctgt tttaggcgta atgggtattt
    14761 taacagcttt tttcaatcaa cgctttattg ccttggtctt tttactgatc gtaattattg
    14821 tttctgccac cttcagtatc cgatttgcaa aaaaaattgc tgaaccaaag cagtaaataa
    14881 ttctcttttt ccctaaagtg ttctacaata aaagagtaca atacgattga cttgaggagt
    14941 gaatgcttat gttacaacaa tacaaaaaaa tcatggttgc cgtagatggt tctgatgaag
    15001 ctgaattagc ttttaaaaag gcggtcaatg tagctatccg aaataacgga gaacttctat
    15061 tagcacatgt cattgacaca cgttctttcc aaaccgtttc atcctttgat ggaatgcttg
    15121 cagaacaagc tactgaaatg gcaaagcaaa cattggcaga ttatgaaagc aatgcgaaga
    15181 aagctggctt gaataatgta acctctgtcg tagaatatgg ctcaccaaaa caaattattg
    15241 ccagagaaat cccagaagat aatcaagtag atttgattat gttaggagcc acaggattga
    15301 atgctgtaga acgtttattt attggttctg tttctgaata tgttattaga aacgcagctt
    15361 gcgatgtgct tgttgtccga actgatttag aaaaccaatt gccagctaaa gaccacacca
    15421 aagcttaaca cgtagacgac ctgccaacta tcggcaggtt tttttgcaca aaaaaccgtg
    15481 ataaaaacaa aagacgcggc aagcactcat cggtctttat aatgtttaaa ataaagggaa
    15541 aacagtcaat gagttcaagt aaaataaaaa aaagtgaaca ccagggcgat tagtcgccct
    15601 ggtgtggttt aaaacgacct aagcatgcca aagcttattg gttcgtcaat gtggaagttt
    15661 ccgatgaatc tgatacgaaa aaatatgaag tatcgatggc ggataccgat tttatcgcca
    15721 aactaaaatt ttatttcggg ttcgggtacc gcgagaaggc aagtgctaca ttcggtaaat
    15781 cattcaagaa ttgatgaaag gctaccgctt gccattgcaa ccacaacgat actcctgaac
    15841 gcgatgttgg tgagatttcc aaagagaaga gctgtttaat gcaacggtat taagcaaatg
    15901 gcaaaaggaa gtcatgtaga caaagttttt tatcaaacac catatgattt cgcctgaacg
    15961 ctggtttagc tcagaataca atgatgtggt ccacgagaca ttaccctcag tcagataaaa
    16021 aaagctctct gacagaatta cacatcgata aatattaagt aaatgtcagc gctagttgaa
    16081 ctttctgaca ctttcaggta cactggaaaa gacaactaca cgaaggaggg aaaatactat
    16141 gatgcgcatg actaaaataa caagtaatga aaattaccaa cgataccata tcgcatttag
    16201 ccatgcaatt ggttttacag ttgctgttga cgggacaagt ttgtccaaaa cagttgaatt
    16261 cacgtaaaat caatggccat ctcaaggtat ttttctcatg ataatctgag tttaaataac
    16321 acaggccatg gataaccatg gcctttttga gtggtctcaa ggagagagat catgatcata
    16381 caagcaattt ctattaaaaa aacaatcggc gggaatccat tatttgagga tttatcctgt
    16441 caaattgaag ctggtgaaaa aattgtctta gtcggtatca acggaactgg caaatcaacc
    16501 tttttacaaa tcttgaccgg cagagaaggt gtcgacagcg gtgttatcag tcgcaaaaaa
    16561 ggcttaaaga tcgggacggt cgaacaagaa cttacagttt ccgaagcgac cgtcaaccac
    16621 tacttgctcc attctgccgc tgagatacag gacctcaaac agcaaatgag tcgctacgaa
    16681 gcattgatga cagaaccaga tacaaactta gagaagtgtc tggcccgtta cggtgagctg
    16741 cagcatcgct ttgaagaact aggcggttac gtattagaag accggattgc agcgatcttg
    16801 caaggcttag ggattccaca ccgtcaggaa gcacggttaa cagaacttag cggaggcgaa
    16861 cgagttaaag tcgctttagc caaaatttta gcagccgatg ccgatttact tttactcgat
    16921 gaaccgacca atcatttaga tcttaacagt attcgctggt tggaaaacta tttacagaat
    16981 acaaaaaaat cggttctggt cgtttctcat gatcggcaat ttctcgatca agtcaccaca
    17041 aaaacatggg aactggaaga aggtgcattg attgagtatc ccggaaacta cagccgtttt
    17101 cgagtcttga aagaagctcg cttggctgag ctgacaaaaa attacgaact gcagcaaaaa
    17161 gaagtccagc ggttgaaagt catgattcgt cgttttcgtc agtgggcaca tgaaggagac
    17221 aatgagtcct tcttcaaaaa agccaaagaa ctggaacgcc gcctggcgaa actgacactc
    17281 gccaaaccgc cacctccccc gaaaaatcgg ctgcaaagct tgtctaatgg tggaaaatct
    17341 ggcaaagaag ttttcatcat tcaaaacttg caccagcagt acgctgatca agtactattc
    17401 aaagacagca gttttgcagt ctatcgtgga gatcatttag ccatcatcgg cgacaatggt
    17461 gcaggaaaat caaccttact caaattatta ctcggtgagg aaaagcctta cggtggcacc
    17521 atcaaattag gtagtagctt gcaaatcggt tatttgccgc agcagctgca gtttgccgat
    17581 cccgatagcc gtttgctggc ttacgccatc acattgactg gaaacgaaga agtcgctcga
    17641 cggcaattag ccaaaagtgg cttttatcaa agcgatgtgg gtaaacggat caaagattta
    17701 tccggtggtg aaaaaatccg tttggcgttg ctgaaactct ttttagaaaa aatcaacgtc
    17761 ctgattttgg atgaaccaac caatcacctc gacagctatg ctcgagaaga aattgaagaa
    17821 atgctgcaag actatcaagg aacgctccta gcagtcagcc atgaccgcta ctttttgcag
    17881 caacacttcc aagaagcact cgttattgat caggagcaga tcagccgcca gccgctcggc
    17941 caactgattg actaggacgt tttttcgcaa caaaaaactt gattttcata cctgtgcttt
    18001 cacgattcag gcaaagtaca ggtggaaaat caagttttta ttattcgatc aatgaatgtt
    18061 tcggatcggt tttctagcta aacataaagc gaagaataaa gtcgacagcc cacgaggctg
    18121 atctaaaaat gttttttctt tttacatatg taatgtccct tataaagatg aatgaaaata
    18181 aaatattggg attttcctga aaatcaataa ataaaatgcc agtcttttcc cttttgacca
    18241 cttttccaag caaaattccc taagaagatc ctgtaaaaaa gaagggaaat tgaagttaaa
    18301 ttaccatttt gcggtcttct ccgcaaaaaa aacacatgca agaattccct cttgcatgcg
    18361 tttcatcttt tatcctaacg aatgctttgg cttagccgcg cagacgctcc acatcacggg
    18421 caatcattag ttcctcatct gttgggatca ctaatactct tacttttgat tcttttgtag
    18481 agatatctac ttcttcacca cgcaattcat tcaacgctgg atcgatttca caaccaaacc
    18541 aagacatacc tttgatgatt tcactacgta catgtccgtc attctcgccg ataccagcag
    18601 taaagacaat tgcatcaaca ccattcatcg ttgtcacata accgccgatg tattttcgga
    18661 tgcggtccac gaaaatgtca taagctaaac gaatatgttc tttttcatag ttcttttcaa
    18721 gatcacgcat atcactagac aatcctgtta ggcctagcag accagatttc ttgttcaaaa
    18781 cattgatcat ttcatcaata tcaatctcta atttttccat taaatatggg agaacagctg
    18841 gatcgatatc acctgaacgt gtacccattg tcaccccagc taatggtgtg aatcccatag
    18901 aagtatccat ggatttacct ccatcgactg ctgtgatcga tacaccattt cctaagtggc
    18961 aagtgatgat ttttagttct tcgatcggtt tgttcaataa ttttgccgca cgttccgaca
    19021 cataacgatg acttgtgcca tggaagccat attttcttac tttgaaatct tcataataag
    19081 acaccggtaa gctatataaa taattttgct ttggcattgt tgagtggaaa gatgtatcga
    19141 atacagccac actcaaaatg tcaggtaaaa tctttttaaa cgctttgatc cccattaagt
    19201 ttgcaggatt atgcaaagga gcaaattctg ctaattcagc aatgcggtcc attactttct
    19261 catcgatcac tactgaatcc ccataagttt ctcctccgtg aacaacacgg tgccctactc
    19321 cggtgatttc atcataagag cttaaaattt gcagttcaat caactgatct aacagcattt
    19381 ttactgctac ttcgtggttt tcgatatcca tgatttgttc gaatttttga ttatcgccgt
    19441 acttgatggt aaaaatagaa tcttgtaaac cgatacgttc gacgatccct ttcgcaacta
    19501 cggtttcttc tggcatttga tataattgcc atttcaaact tgaactccca gcattgattg
    19561 caattgtttt agacatatga ctctccttct cgttgaatga ttacagagca gatgatttcc
    19621 agtttttaaa ctcgttgaaa aattccgtca ccttctctgg ttcttttaat gaagcaagtt
    19681 tcacaagcaa gacttctttt acttgttgtg cttgtggtcc ttttttctgt aagatcaaaa
    19741 tacttttttg cgattgttta ttacggaaca attcatctgg caattgaatc attccttgaa
    19801 gataaccttc ttctttaaac cattttttga tttcttcact ttgatccgtt tctaaaaatc
    19861 cactcggcat caaaaacaaa ccaaaaccat ccggcttcac atatttcatt gattgctcta
    19921 gcaataaatg atgtgcataa ctatggcctt cttcggtact tgttaaaaac tcttttgctt
    19981 tttcgtcatt cggataataa ccgattggca aatcgctgat tgcaaaatcg acaggatcta
    20041 ttaataattc ctgcaacccg tcttgatgaa agtattgaac atttgcttgt gtcaaatcac
    20101 ttgtagaagc tgcgacagcc aacaatgtat catcaatatc cacaccaatt ccttctgttt
    20161 ggtaccccgc attcgatagg tttaaaagaa ctgttaataa caagttgccc attcctgccg
    20221 caatatccag aatcttaacc ggtgcttttt tatttgtgta tagttgttca ataagaaaaa
    20281 caaataaaaa accgatacta tcaggagtga gttgatggtt cgcctgcaaa tgttctgtct
    20341 ggctgccctt caataaaagt aattgagaca gtctgcgcca ttcttctggc tcgaaagaaa
    20401 gtttcttcaa ctcttcataa agagctgtga tccgttgcgt tgtctctttt gtcggcactc
    20461 catccaccac acgtacttga taatcatcta ttaaattttc tgcattttcg acataggctt
    20521 ctaaaaagga agttcccaat gcattctgta aaagctgaat agcttccagg ttttgattaa
    20581 acccttgttc cattttttct ggtaacatgg tgcacctcat ttctagatat attataacaa
    20641 ttttttcacc tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt ttcactgttt
    20701 tttatttgtg aaacggcaaa cattttttta ttaaaaacag tgttataaca gaaaagaggg
    20761 cgttttcacg aacattccat cattctgtac tagtatctgt atcatttttt acttcttctg
    20821 tgaaccgaaa gataaacggt gagacttcca cctctaagaa tagggactgt tgtacccact
    20881 gatatgttac agatccagtt gtgtagtaaa aaactccttc cgaactttcg gcaagcgtag
    20941 actgttccga taaagccatc tgcttcatta tttttgctat ataataattt ttgattcgta
    21001 tattaagatc cattgacaaa cggtagcttt ctaatgctgc catcaataaa aacgtacata
    21061 aaagaaataa aagcaagaca gaaaataaaa tagagccctt ataacatttt ctactgatcg
    21121 ttttttgtcc atatggcata tccatcttct ccattttcaa agtgtacata aaaagaaatt
    21181 gtgtgttctt tttccaataa ttgaagctca gtgatacttg ttaacatagg ttgatgtccg
    21241 ccggaagttc ttcttttacg tatcattgcc tgatatcttt cgatgatata ctgtttatcc
    21301 tcgatcgtgt agtgcagttg ctgatttttc acagagacat tgtgactgct cttgagttct
    21361 tcttctaatt gtgctaaaaa tattagccac tctttttgat ctttacgctg caaaaattca
    21421 cttacttgga tggtttgctg aatcaaaaga ttgaatgtga gcaatagact gctcaatatc
    21481 atcagtccaa ttagacattc aatcagcgta aatgctggaa ttacttgttt tttcaatact
    21541 gatcgcttct ttctccatat tgacaaacac tttttcgatt actccattat tatcgacaat
    21601 ttggatatcg tatgggatca tttcttccct tcgctctggt cttttgtgat acatccgctg
    21661 cgtcttgata tcttcataaa tcattcgaat aaatagcagt tcctccccac ttttcttttc
    21721 tgcctgaagc aaaaacagct ggccaaagct cactaagtag acacagacag aagcgatcat
    21781 aagcgctgta agggactcca acaaaacaaa tcctgtttta ttcaattctt tttgtatagc
    21841 gaccactccc tatctggaat tgatacgtaa tcgtccgctc tttttcttcc caataaaatt
    21901 gataagcttt tagtgaactt tcattcccgg ttctagcagc aaaagtaata gaagcatggc
    21961 gtttcaaggt aatctcatca ggaatatcta atacttccca atttatttta tctggattcg
    22021 gaacatgaaa aatgatttga ttctcttcac tattccaata aaagcctgtt tggatctgat
    22081 tcacaatagc gattttttgc gtagaataga ttcgtttttc aaattggccg aaaaattgat
    22141 agactgccaa ttcattcttc catgcagaaa atgcgagaac tggaaaagaa aggatcgttg
    22201 taagtaataa aatcagtaag agtgattcaa acaacgtata tcctgatgct aaaaccttac
    22261 cttcaaggcc gctgatagat ttctgcatgt tctcgtttga tatagccttc attcaacaat
    22321 tcctccacag taggaactct tccattattt tgaagcgcat agagctcttt ttgtgtgtct
    22381 accaactgga tgatcgcttc acgactttct tggtctacat gattcctata acgcgacaaa
    22441 ttagggacga acaacaacac taatacactg atgatcagta agacgattag catttcgatc
    22501 aatgtaaaac cgctataata agatattttt ctctttttca tggcaaaacc tcctctatgc
    22561 ttccgtaaac aggtaataaa acagcggcat aaactaataa aatcaaagcc gctacgccca
    22621 aaaaaatgat tggctgaata aaatgcatcc agtgatgaag acgtttgaaa agctgttttc
    22681 ttgtccattc gctgtaaaaa atcagttctt tacctaaact tccctttgcc tctccctgta
    22741 aaacaatttt actgaattct tctgatagaa aacgataatt ctggaactgc tcagccaatg
    22801 acatacctgc ctccaatcct tgaatcaact tagaggcaag atgctgaatc agactttctt
    22861 gtttcgtttc ctttaagcag actatgattt gccttaattc gaaaccttca tgaaaaagct
    22921 ttccaatctc tagtgacaaa taagccgatt gatacaagga atatatcggt ccaatcaata
    22981 attgtctgct tatccattca ctctgatcaa caggagacaa cttcatcaga cgatacctta
    23041 ctagtaaagc caatagagta accgctagga tgaatagtag aacataccaa tgaaagactt
    23101 gtatcagctg aataccccaa tgctcttgca tgaccattcc tgaaccaata agctgaggca
    23161 aaacgaacaa ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat agcatcggat
    23221 atgcaagcag ctttttcaaa tcattgcgaa atccttcaac aagccgaaac tgtttggcaa
    23281 tttccaacaa agttgtcggc agattcccat gacgttcagc taactcgatc tgtaccagtt
    23341 gctctgctcc atatcctatt tgcgaaagta cctcactaaa cgcttgccct tcctttagct
    23401 gatgttggat atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt aataaataaa
    23461 gggattcttg cagactaaaa ccagataaaa gcagttctcc catggtttgt gcaaactggt
    23521 tttgctggct tcttgttaat cctttagaat agttctgact gccaaatatt ttcctgagct
    23581 tcttcataac tttcctccca tgattttttt actgattctt cctccataaa ttgataactc
    23641 cataaaacag aaggatggtg attggcatct tccaaaagtt cttgataaat gacaccagct
    23701 aaacattcag atagctcttc ttttttcttt gtcagttcat ggattctgac caatgtacct
    23761 tcaagattgc gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc tgctcttacc
    23821 gcagcatgtg cagttgattc atctcttatc tcgccaatga ttagcaagtc tggacgatgc
    23881 cgtaatgcga gtctcaaaag tgtatcataa gtctgtgcta ttttcggatt gatctgtagc
    23941 tgcagaaaaa acggttcttc tatctctaca ggatcttcga tagtaatcac ttgcaatttt
    24001 tcttccaaag ccaaccgata cattagagaa gtcttgcctg atccgactgg tccgctgaaa
    24061 aggtataatc cacgtctgac ggttttctgt aaaatagttg caaaatctcg agaactaaag
    24121 caacgatggg gtgtttcaaa agtaccgtat aatagccgaa tcactaaact ctctcgttga
    24181 aggtaatctc ctacagaaga caaccgcaga cgaacttcct gtttttctaa agaataagta
    24241 aatgcaccta actgagcttt tcttttttct ccgatatcca tctgtcccaa atacttaaat
    24301 cttgagatca gttgctgtcc atctgccgaa gagactttct tccagaatgt tcgttttttt
    24361 ccatagcgaa aatagatgta aaatacttct gaagctggca atatatatac gtcttgggca
    24421 tgtttggcaa aactttcact tatcatttga tccgataatc gttttatttc cattattctc
    24481 ccctccacaa aaaacatacg tctttttgat ctttttgttt caaataaaat gaatttacaa
    24541 aaaaatattg gcaaaaaccg aatttatgtt atacttataa aaatttatct tgggaggcac
    24601 tccattggaa acaacattaa ggaaatatat cgaagtagca agtaaaaaaa agcctgctga
    24661 cctagtgatc aagcatgcta agatcgtcaa cgtctttacg aaagaaatca tggaaaaaga
    24721 tgtagcaatc tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa aaagcgtaca
    24781 taatgctaat gggcaatatc tagtgccagg attcatcgat ggccatgtgc atatcgaaag
    24841 ctcactttta tcaccaagcg aattttcaaa agtatccttg cttcatggag ttactactgt
    24901 agtgacagat ccccatgaaa taggaaacat tgctgggagt acaggtcttg agtttatgat
    24961 agaagatgca agacaaacac tcatgaatat cttttttatg ctaccttctt gtgtacctgt
    25021 aacccctttt gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc cttttttaca
    25081 aaaacccgaa gttttaggct tagctgaagt catgaactat caagcagtag caaataatga
    25141 aacagacatc ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa ttgacgggca
    25201 tgcagctggt atcggcatgg aagagttaaa tgtttatcct gcagcaggta ttcggacgga
    25261 tcatgaagct acaactgcaa aagaagctaa agaacgtttg gatcttggta tgtatttaat
    25321 ggttcgagaa gggaccgttg caaaagatct ggcatcgcta ctaccggcga tcacacctga
    25381 aaattcaaga agatgcttct ttgtcaccga tgataaactg atcaatgatt tagtaaaaga
    25441 aggatcaatc gatcatatcg tccgtcaagc tattgcactt ggaatcgatc cattacaagc
    25501 cattcagatg gcaacattga atgcagcaga atgttttgat ttgaaaacac taggtgcgat
    25561 tgcccctggg tatcaagctg atttcttctt aacagacgat cttaccaagc tgccaattca
    25621 tgaagttttc tataagggaa cattagttgt tcaatctggg caactccaag accatttatt
    25681 tgctgatcat tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc aaccattaaa
    25741 tccacattcg ttagagctgc ttctttcttc ttcacaagcc catgtgatcg aaattatccc
    25801 gaatagtcta gtgactaaag atttggtaga aaaagtggac cgacaaggcg aacgattcat
    25861 tccttcaatc gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc atcaaacggg
    25921 aaacattgga ttaggcatag tgaaaggatt tcagctgaaa caaggagcga tagcgacaac
    25981 gattgcccat gactcgcata atctagttgt cgttgggaca aacgacgagg atatgctgta
    26041 tgctgctaat cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca atcagcagga
    26101 aatagcttgt gtttctcttc ctataggcgg aatcatgaca caggaacctt tttcgatcgt
    26161 ctatgaacag ttgaatgaat taataactaa agcacatcaa ctaggagcag ctaaaacctt
    26221 tgatcctttt ttgacacttt ctttcttaac attatctgtc atacccgaat tgaagatcac
    26281 cgacaaagga ttattttcat tttctcgctt ccaattgatt ccaccttgtg gaaaaaccga
    26341 tgaaataaat acattttaaa tcaaataaat cccagaaaat acattacatc tgtaatgttc
    26401 attttctggg atttatttat ggatattaat gtttttatga aagcagaaag ttagtttcac
    26461 agacttttct ttttggtctt atcttgaaat tgagcttcta ctcgataaat acgttgacct
    26521 tttgaagaaa atttttcttc atactccgtc ataatattgc cttcaaattg actttgatgt
    26581 aaatctaacc atacctgttt gaggatcatg ccatattgag aaaaacttgc cagagaatac
    26641 tcaaacaatc cttgattatc tgttttgaaa tggatttctc catttggacg aagaatctgt
    26701 tcatctacag caagaaaagt cttatatgtc aatcgccgtt tttcatgacg tttttttggc
    26761 catggatcgg aaaaattcag atagatttga tctacttcgc tgtcagcgaa atactctgtc
    26821 aacgctgatc catccacatg tagaagtttc aggttaggaa gcggttgttc gatcaacttg
    26881 tctagtgcaa tcgaaacaac actgacctgc atttctactc caatataatt gatttcagga
    26941 tgggcttttg ccattccagt gatgaactgg cctttcccca taccaatctc aatgtggatt
    27001 ggatgatcgt tttcaaacaa ttcattccat tttcccttcc acaatgttgg atcactaatt
    27061 acaaaattcg ggtgagccgc taacatttcc gctgctcccg gacgatttcg gacacgcatt
    27121 atttatctcc attcctttcc tctaattaaa aaggtctggc caaatctccg cagagtttgg
    27181 gccaaacctc agccactgaa aattcgtttt aattgtaaaa tttcttcgtt catccgtcga
    27241 tgatcttcac gtttgcaata tctggtgatt tcttggagca aattcatggc cgcataccaa
    27301 tgaaattttt caagcatttc gtcagtcggg cgtatgccat aactgatcag ccactgattc
    27361 cagctagaca gtggcacata atttcctaaa atcgttgcaa tatccaccgc agggtctgca
    27421 aacatgattg aatcccaatc gactagatat aaataatttt tacatactaa ccaattacga
    27481 tggtttacat ctccgtgtac tactgataag ttttccttat ctattgacgg gatctgctca
    27541 cgaagaaaac gatggacaat ttgcagaaat tgatttttct ggagttcttc cggtaattct
    27601 ttttcatatt tttgcagcat cttctccggc gtcatgacac gaccaccgat acgggtcagc
    27661 atggttttaa gagaatttga atgatgtaaa tggtacaaaa catcaatcac gtcgtttctt
    27721 tgaccaatct cattaggagt cagcaagcga ccttccagcc actcttgtgc tgtcaaagta
    27781 tcgccattcc ccgttcgttt tgtccaaaca agttttggtg taattccttc ccttgaaaga
    27841 gcagccaaca tcggagtcgt gtttcgttta ataaagactt tgtcctcatc acgcatgcct
    27901 atgtatgttt ttcctgtgtc gcctttgatg ggacgtaacc gccactcttg gtctaactga
    27961 aactccatca tgcattctcc cgtcctctta ctctttgttt tgcagtactt attattctag
    28021 tcgcctgaac actacacgtc aagcacatat acgttctttt tcatttaatt gccattcaaa
    28081 aaaccagctt ttctcaaaaa aaatagaacg aacagcattc ttactgtcaa cgctctattt
    28141 ttcattctca tttttaccct acacagcttt gttttcagtc tgacatcttc ttcaaacgag
    28201 ttggtacata tagtttaaca aagagcatag taacaatcaa ataagccgct agcggaaccc
    28261 atcgctgctg cccatctaat acgactgcag caatcaaacc aaaaataacc gacgcgataa
    28321 ttaataccca tgtgagtaga tactgaatcg cctgcttctt ctgattctta tggattggat
    28381 aaagctgaac gagaaccata tagcggaact ggttcgctaa aggtaacagt tggaaaccga
    28441 tcaaatataa aaataagaaa ccgataatca gactaaaata aaagtcgact actccggcaa
    28501 tcaataaccc acctataatt actaatcgga aataaagacc actaaaatca gaacctcttg
    28561 ccaatcttct tgcaaacaag taaagataag tgttttttga ttctctttta atatgagtca
    28621 atataggatc caaatagcgt cttcttttga tctttgccgt aatttcagga acatctgtga
    28681 ataaattgat aaattgatag atctgatgca acctatgttc ttctacatgc accattttct
    28741 cccaatccaa tcctttttct tgttgcttcc acaaaaacca gtaatataga cttgcctgta
    28801 ctaatgcacc tgctattcct gcccatatta gccaataaaa tgatagaaac aaaaagatca
    28861 gagaagaaaa aaaccagcct aatttccaat aacgaataac ttgttgatct agtcgaaaca
    28921 attctgctct cttgatttgc agatgactga atttcaaact ccataatatg atcagataga
    28981 cgaaaaacat cgaaaaggat tgctccgtac taaccaccac taatggcatg gccaatccgc
    29041 atgccaatat caaaaagatc aacggaaaaa tgcaagaata gcgaaaagct cgttccaaat
    29101 aggtttgcat ttctttttct ttaggaagta aaaaaacaat atctgctgcc tctgcaaaag
    29161 tagccatctt acccacgaat aaacagagca tccaaaatac agagattatg agccctccaa
    29221 ttgcaaacgg tggctgcagg gttttcaacc aattagaata atataatccc acaccgccaa
    29281 tcaagaaaag acaaactaaa acaaaatgat cgttcaatac ataacgcata tatttcatca
    29341 ttttcttttg atgtcttgct aaacgttttt gaaaaaaact actcatgtgc ctgctccttt
    29401 gttaaagcaa tatagatttc gtccaaagaa gaatccggca gctgaaattc ttttctcaac
    29461 atctcaagag aaccgtctgc tcgtatctct ccgttatgta agaccacaaa acgatcacaa
    29521 tatttttctg cagtagctaa aatatgcgta gacatcaaaa taccggctcc ttcttttttc
    29581 atttctacca tcaattcaag caatgcattg atagctagtg gatccaagcc taaaaatggt
    29641 tcgtcgatga tatataaaga aggctcgatc aaaaaggcgc ataagatcat cactttctgt
    29701 ttcattccct ttgaaaaatt agctgggaac cagtcaagac gattagataa ccgaaaagtc
    29761 tccaacaact tttctgctct tttcattgct atatccatag gtatatcgta ggccatcgca
    29821 gtgacttcga tatgttctct caatgtcaat tcttcatata aagaaggagt ctctggaata
    29881 tacccaattt gtttgcgata ttcttccgta tttttcttta aggatagatg attgatttct
    29941 atcgttcctt ttgaagcttc cagcagaccg ataatgtgct tgatggtcgt acttttccct
    30001 gctccgttca gtccaatcaa ccctactatc tctccaggct gtacatcgaa actgatatct
    30061 tttaacacag ggagatgtcc gtacccccct gtgacatgat cgataattaa actcattctt
    30121 tttcctccgc catttatttt tatttattat accacgaaga aaatgacggt attttgtttt
    30181 tttccttaca atagctttaa cttatggtaa agttaagctg aagtatttat tctttataga
    30241 aaggatgttt tatatggaaa actgtatttt ttgcaaaatc atcaatcaag aaatcccaag
    30301 ctataaaata tatgaagacg acaaggtcta tgctttttta gacatctctc aagcaacgaa
    30361 aggacacaca ttagttgtgc ctaaacgtca tgtagcagat atttttgaat atgatcccga
    30421 acttgcagga gaggtttttt cgcgtgtgcc taagattgct caagcattgg aaaaagcttt
    30481 tccagaaatg aaaggtctta atatcttgaa taacaaccgt gaactagcct atcaatctgt
    30541 ttttcattca catatccatt tagtccctcg ctacagcaaa gaggatgatt tttctattca
    30601 ctttgtcaat catcaagata gttatggatc ggaagaacta aaagctattc aagaaacaat
    30661 cgtaaagcag gtgtcctgcg atgattaaaa actttttcaa aggactgtta tttggagctg
    30721 ctgctggcac tgtcggaggt ctgttattgg cgcccaggag cgggaatgaa acaagaaaga
    30781 aactgatcga agaattggaa gaagcaacaa ctctgacaaa tgatttaaat aacagcctga
    30841 atcattttaa aagcgcattg atcgaaacaa aagaaacagc tgaatctgtg attcctgctt
    30901 tccaagaagc aatcaaaaaa gatatcgaag attttaaatt ccaagccgaa ccacgtatca
    30961 cacaaatcaa tgaacaagta gaaaaactaa aagctgatct gccagagtat cctgaacaaa
    31021 tataataatg attattttct caacagattt aactatataa gtgagaaaag tttgcgtatt
    31081 tcctaggaat gtgatgaaat tttgaatttt gtttgaaact accacgacaa cgtttttaaa
    31141 ggaaatggga tatggtatag ttaatatata aaaataataa ctaattacag gagtgcaaat
    31201 tttaatgaaa aaaaaatcaa ttattttggc agctactagt gcgttggctg ttttaactct
    31261 agcagcttgt tcaggcgata ctaataaaga tatcgcaacg atgaaaggcg gaacgatcac
    31321 tgtatctgat ttctatgatg aagcaaaact tgaatcatcg aaccaatcct tagtacaacg
    31381 gatgatcatt tataaagtat tcaataacaa atatggcgat aaagtaaccg ataagcaagt
    31441 ggatgcagaa tacgacaaac aagcgaaatc attaggtgat acttttgaat cacaattaga
    31501 agctgctgga tatacgaaag atacttataa agaatatatt cgtaacaatt tagcttttga
    31561 agctggcttg aaagctcacg tagatattac agatgacgat ttgaaaactg cttggaaatc
    31621 tttccatcca gaagtagaag cacaaatcat caaattatca agtgaagatg aagcaaaaga
    31681 tgtgaaaaaa tcagcggatg atggagatga tttctctaaa ttagccaaag acaaatcaac
    31741 tgatacagag acaaaagaag atggcggtaa agttaaattc gactcaacga caacaactat
    31801 tcctgcagaa gtaaaagaag cagctttcaa attgaaagac ggtgagatct ccgatgttat
    31861 tactacaaca aatcctactt cttatgcgac agaatactac gttgtcaaaa tggtgaaaaa
    31921 ccaaaataaa ggcaatgaca tggataaata caaagatcaa ctaaaagata tcgctactga
    31981 aactaaacta agtgacaatg cattcacaac taaagtaatc ggtgaagaat taaaagatgc
    32041 aaatgtaaaa atcaaagatg atgcctttga aaatgtgttg agtgcattca caacaacttc
    32101 atcaagtacg aaagactcaa gtgaaactac agcttcaaca aagagttctg acacaaaatc
    32161 aactgattca acaaaagaat cttcaactga agaaacaact gattcatcaa aataaa
//
LOCUS       DAYYFD010000028        31599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000028 DAYYFD010000000
VERSION     DAYYFD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.028"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHA25_001153"
     CDS             complement(<1..1835)
                     /locus_tag="ACHA25_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8762457.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHA25_001154"
     CDS             complement(1832..2536)
                     /locus_tag="ACHA25_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8762458.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHA25_001155"
     CDS             complement(2548..4857)
                     /locus_tag="ACHA25_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8762459.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHA25_001156"
     CDS             complement(5052..5516)
                     /locus_tag="ACHA25_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762460.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHA25_001157"
     CDS             complement(5516..6142)
                     /locus_tag="ACHA25_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8762461.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHA25_001158"
     CDS             complement(6149..6514)
                     /locus_tag="ACHA25_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8762462.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHA25_001159"
     CDS             complement(6504..6842)
                     /locus_tag="ACHA25_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762463.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHA25_001160"
     CDS             complement(6832..7158)
                     /locus_tag="ACHA25_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8762464.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHA25_001161"
     CDS             complement(7139..7417)
                     /locus_tag="ACHA25_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8762465.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHA25_001162"
     CDS             complement(7419..8783)
                     /locus_tag="ACHA25_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8762466.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHA25_001163"
     CDS             complement(8796..9365)
                     /locus_tag="ACHA25_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8762467.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHA25_001164"
     CDS             complement(9337..10524)
                     /locus_tag="ACHA25_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8762468.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHA25_001165"
     CDS             complement(10588..12315)
                     /locus_tag="ACHA25_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8762469.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHA25_001166"
     CDS             complement(12312..12764)
                     /locus_tag="ACHA25_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8762470.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHA25_001167"
     CDS             complement(12876..13256)
                     /locus_tag="ACHA25_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8762471.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHA25_001168"
     CDS             complement(13253..13639)
                     /locus_tag="ACHA25_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8762472.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHA25_001169"
     CDS             complement(13641..13919)
                     /locus_tag="ACHA25_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762473.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACHA25_001170"
     CDS             13967..14695
                     /locus_tag="ACHA25_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8762474.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="ACHA25_001171"
     CDS             complement(14826..15302)
                     /locus_tag="ACHA25_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8762475.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15618)
                     /locus_tag="ACHA25_001172"
     CDS             complement(15262..15618)
                     /locus_tag="ACHA25_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762476.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="ACHA25_001173"
     CDS             complement(15596..15781)
                     /locus_tag="ACHA25_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762477.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="ACHA25_001174"
     CDS             complement(15782..16054)
                     /locus_tag="ACHA25_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8762478.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16251)
                     /locus_tag="ACHA25_001175"
     CDS             complement(16051..16251)
                     /locus_tag="ACHA25_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762479.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16379..16591
                     /locus_tag="ACHA25_001176"
     CDS             16379..16591
                     /locus_tag="ACHA25_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762480.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16624..16818)
                     /locus_tag="ACHA25_001177"
     CDS             complement(16624..16818)
                     /locus_tag="ACHA25_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762481.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16831..17013)
                     /locus_tag="ACHA25_001178"
     CDS             complement(16831..17013)
                     /locus_tag="ACHA25_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762482.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17010..17150)
                     /locus_tag="ACHA25_001179"
     CDS             complement(17010..17150)
                     /locus_tag="ACHA25_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762483.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17640..18005)
                     /locus_tag="ACHA25_001180"
     CDS             complement(17640..18005)
                     /locus_tag="ACHA25_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762484.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18045..18347)
                     /locus_tag="ACHA25_001181"
     CDS             complement(18045..18347)
                     /locus_tag="ACHA25_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8762485.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18347..18646)
                     /locus_tag="ACHA25_001182"
     CDS             complement(18347..18646)
                     /locus_tag="ACHA25_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762486.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18639..18803)
                     /locus_tag="ACHA25_001183"
     CDS             complement(18639..18803)
                     /locus_tag="ACHA25_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762487.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18809..19078)
                     /locus_tag="ACHA25_001184"
     CDS             complement(18809..19078)
                     /locus_tag="ACHA25_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762488.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19090..19839)
                     /locus_tag="ACHA25_001185"
     CDS             complement(19090..19839)
                     /locus_tag="ACHA25_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762489.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19836..20528)
                     /locus_tag="ACHA25_001186"
     CDS             complement(19836..20528)
                     /locus_tag="ACHA25_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8762490.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20534..21205)
                     /locus_tag="ACHA25_001187"
     CDS             complement(20534..21205)
                     /locus_tag="ACHA25_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8762491.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21198..21539)
                     /locus_tag="ACHA25_001188"
     CDS             complement(21198..21539)
                     /locus_tag="ACHA25_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762492.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21717..22415)
                     /locus_tag="ACHA25_001189"
     CDS             complement(21717..22415)
                     /locus_tag="ACHA25_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8762493.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22502..22972
                     /locus_tag="ACHA25_001190"
     CDS             22502..22972
                     /locus_tag="ACHA25_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762494.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23015..23194)
                     /locus_tag="ACHA25_001191"
     CDS             complement(23015..23194)
                     /locus_tag="ACHA25_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762495.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23181..23519)
                     /locus_tag="ACHA25_001192"
     CDS             complement(23181..23519)
                     /locus_tag="ACHA25_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8762496.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23535..23738)
                     /locus_tag="ACHA25_001193"
     CDS             complement(23535..23738)
                     /locus_tag="ACHA25_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762497.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23753..24529)
                     /locus_tag="ACHA25_001194"
     CDS             complement(23753..24529)
                     /locus_tag="ACHA25_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8762498.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24828..25148
                     /locus_tag="ACHA25_001195"
     CDS             24828..25148
                     /locus_tag="ACHA25_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8762499.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25166..25597
                     /locus_tag="ACHA25_001196"
     CDS             25166..25597
                     /locus_tag="ACHA25_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8762500.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            25727..26188
                     /locus_tag="ACHA25_001197"
     CDS             25727..26188
                     /locus_tag="ACHA25_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8762501.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            26222..26857
                     /locus_tag="ACHA25_001198"
     CDS             26222..26857
                     /locus_tag="ACHA25_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8762502.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            26970..27605
                     /locus_tag="ACHA25_001199"
     CDS             26970..27605
                     /locus_tag="ACHA25_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762503.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(27598..27732)
                     /locus_tag="ACHA25_001200"
     CDS             complement(27598..27732)
                     /locus_tag="ACHA25_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762504.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            27904..29043
                     /locus_tag="ACHA25_001201"
     CDS             27904..29043
                     /locus_tag="ACHA25_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8762505.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            29325..29555
                     /locus_tag="ACHA25_001202"
     CDS             29325..29555
                     /locus_tag="ACHA25_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762506.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            29561..29860
                     /locus_tag="ACHA25_001203"
     CDS             29561..29860
                     /locus_tag="ACHA25_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8762507.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(30375..30448)
                     /locus_tag="ACHA25_001204"
     tRNA            complement(30375..30448)
                     /locus_tag="ACHA25_001204"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30412..30414),aa:Ile,seq:cat)
     gene            complement(30637..>31599)
                     /locus_tag="ACHA25_001205"
     CDS             complement(30637..>31599)
                     /locus_tag="ACHA25_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8762508.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT         9369 a         6230 c         4984 g        11016 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
    16321 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
    16381 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
    16441 attaaaaaaa gataattctc cagaattatt ttctaatttt accgaagacg acattcctac
    16501 tcttaaacaa ttaaaatatt attctgatta ttggacgcaa actcgtgaga atgaattaaa
    16561 tttagaaaaa ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta
    16621 gtttcaactt ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt
    16681 tagcaacaaa tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag
    16741 ccattggttg gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc
    16801 tgcaagttga atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag
    16861 caatccgaca tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc
    16921 ttgagaaaag agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc
    16981 ctcaaggata attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc
    17041 ttatgcttgg accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga
    17101 tcaattaaag cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct
    17161 tcatctaaac agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt
    17221 tctccacgct cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc
    17281 agaatctgtt actgccggga taatttaata ttcttttcat aattcacctt ttttcctact
    17341 gacatcgaat atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta
    17401 cacatgatgt ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt
    17461 tcttgacgca aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg
    17521 gatcacttca aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct
    17581 tacagcaatg gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt
    17641 tagcggacta cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt
    17701 aggtagttgt ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat
    17761 tccaaaaatt ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta
    17821 atagtgcaaa gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca
    17881 tactccaatt cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt
    17941 agaacatatg cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca
    18001 atcatgtcta tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg
    18061 gacttaacga aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca
    18121 aaatccaagc actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact
    18181 actacatcgc caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca
    18241 acttccccga tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat
    18301 cccttatctt ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca
    18361 catacctaaa ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa
    18421 tatttttgct cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc
    18481 gatcaccatg ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca
    18541 ttgatttacc gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg
    18601 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
    18661 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc
    18721 cacttgtctt cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt
    18781 gctgtaaatg tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt
    18841 gcaagccttt aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg
    18901 ttccatcgat atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct
    18961 taattccttc aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt
    19021 cctcgccctt tttataattc gctatttcat taattagttc tagataatcg atcttcattt
    19081 ttccacctct tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt
    19141 gagataggct tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt
    19201 gtatccactg cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt
    19261 tgccaaatag tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat
    19321 tcgctcttgg taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa
    19381 aagaacattt ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga
    19441 aggcggtaac tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt
    19501 attatctttg actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt
    19561 aattcgtagg tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt
    19621 caagtatcca aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc
    19681 atataaatct gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc
    19741 cgttatctct ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt
    19801 ggctggtata atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac
    19861 tttttgattg ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta
    19921 atcacaccta ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
    19981 tctgcatgat caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg
    20041 tcagctcctg ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa
    20101 tattcttgat tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
    20161 atgaaatcta agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg
    20221 tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc
    20281 cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag
    20341 tttcgttgct gtacggtgaa acgtctagga tcaataaaac gaatttcaat gactcgatca
    20401 ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt tcacttcctc attgatggtt
    20461 gcgcttatgt cttttccttt gaactttttc agaaccgctg aatatgaatc gattaatggt
    20521 ttaaacactc atatcacttc ttatctaatt cttttctctt agctgctatt gctcgctcca
    20581 tcaaggcaca ttgctcatag cttaactgtt caatagtttc aacgttatca gctaagagcc
    20641 ctaatttatc tgtctgctca ttaacatatt cgattaaggt tttggtcata tctttaccca
    20701 tctgctcatt gaaagcttct agaatcgtct ctagcatgtt taatttcttt gtatcgattc
    20761 taggtggtgt tggaatatct tccccttgaa atacatataa tcccagtccg tgtagagcca
    20821 atgctttcac aaagcatcgc ttcaatgagt tattgatttg cattgcattt ggtttaacaa
    20881 ctggttggtt tcgataatct aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg
    20941 ttactgagac agatacataa gtcccagttt catccataag aaaaggttta tattcctcaa
    21001 caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg tttgtactca ttaatagtta
    21061 ccgttgcctg tggatcattc tttttcataa tctcccacgc gtgagcccaa gataaataat
    21121 caaaatttcc ttttttcttg agaattttat ttaacttacg actaaaaagt ttttcaaagt
    21181 tcgttgtccc tttgatttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta
    21241 cctggtccgt aataagagat atcaatcaag ttatctctgt cgtactcttc tagcgcatca
    21301 atcaagccat cttcgatgac gtaaatatat tcaggtttat tcgaatgctt cgatagatgg
    21361 ataagataaa catgatccca aatagttaca tggttgccta aatcatcttg atcccaagct
    21421 agttcttcat tcgtcaaaag atttcgtctg atttttcgac cacttgtttc ctcaattttc
    21481 ggcttgcccc aatcaggatc aatcaaatat tgatctagag tggaaagttc tttttccata
    21541 tgctaaaatc tccttatgat gtgttttctt tgtgactctt tgcttgccgg cggagtcact
    21601 tttttatttg ttgccatgct ttttgcttgt caatatgttg ttggcttagg atgattggtt
    21661 tatagtattt ccaccagcaa ttagcaattg ccgtccctat tcttagcgct tcagctctat
    21721 tcattgtcat caccaaaaag tctctgttgt ctgttcagtt gatcaatttc catacggatc
    21781 gcagtttctg gcaaccacat ttcaataaat gaaacagcat catcgaatct cttacgaggt
    21841 aactcgccat atcttgggat tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat
    21901 acttttttgc tgatttcttc ataagctcgg ctttcttttc cccctagaac tcccataact
    21961 ttcatatttc ctttttgctt aatttcaaac tcttgttgtc cgctaattcg catagtatct
    22021 ttaagcatgg aaacatcttt tttaacatct ttcatttctt ctaattgata gatcatcata
    22081 tcttcaattg tttgaggaac agtattcttg cgaataacat cttccatttc gttgaatgct
    22141 tcaatgtatt tttgtttgaa gtagatagct ttctttcctg taaaccccat agccagcaag
    22201 aaaaatccat ctctactaat gaagaaaact cgtcgatttc tgccatatga atctggttca
    22261 ttaccttcta caaacatctg tccaaaattg gacacatctt tttttagtgc atcaatatct
    22321 cttaaaacat gttgatgttt tttctcgaag ctttctgcca cttgtaagct cgtagtcaca
    22381 gcttctttat ttttcaaaat tactaattct tgcattattt cttctctcct ttttggtata
    22441 atttagttaa aaactggtgg tgtttatttt gaattttttt cacttaacaa tttttcaatg
    22501 gatgtcaata ataagtttta tattgagtac gtttttaaca gggataaaaa taaaaggaat
    22561 gcgccctcaa ctagaagttg aacttaatgc ttcttacttc gctgcagata tgatttatac
    22621 aaaagtaatc atttctaact attcaacaga accagctatg ttagttaatt tagaactttc
    22681 atccgctaac ttaaaccgca gatggtcagc tactccattt aagaaattaa ttgctaaagg
    22741 aggatcagtc gatgataata gaatatactc tgaagctgta ccgctaaata ttcctcctaa
    22801 aagtgctata tcttgttacc ttgcatttga agtagggaaa attaatttta ataaactcct
    22861 taatcaccaa acaaaaatga tatttactct aaatagaacc caaatccaca aaattgttga
    22921 tatcaaaaat acaaacttcc caatagagaa attagtgaaa gaattaaact gagtaatagt
    22981 aatatacatt ctagtttctc ttttctattc atttttagtc agccccctcg gttggctttt
    23041 tcgctctgta ctcagcttca gcttcatcaa gccccataaa aatccaaacc atgtaaacaa
    23101 tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa agcgtagatg attagcggtg
    23161 cgctgaacac tagcgctcta ttaaattttc ccatccgctt gcctccttaa gatttccgaa
    23221 aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa atagatatgt gtctcttcct
    23281 tcaactggat aatagacaaa tccatttttg tttttctcga tatcgataat atttctatat
    23341 cttgggttct ttaaaactct agaagtaaac caatcatatt ttctgttaat ccgttctagc
    23401 acttctggca acgtcatcca tctaccagta tcatcagctt tttttaactc ctcataatcc
    23461 acttgggaga taattacata gccttctgga attgggattt ttgcttctag atattgcatt
    23521 agctgttcct ccctttacaa ttcgtacata gtgataatcg aatctataat tctgtttgct
    23581 tctgcagaag tctttttacc gtttaaaatt aaagataagt agcttttgct aattccaaat
    23641 ctttcagcaa gcatggtgta agttaagaac tttgaacttt cgacatattc tttgattttt
    23701 tctctatctc gttgagtgat ttctgcaatg tcagtcatac taaaactcct ttctaaccaa
    23761 tttcctctaa atccatttga gggtaatatc cttctttttt tagtaattcg tagataaata
    23821 gacgcccttt ctgtgtccat ttggtattca ttacaatttt agtgccacca tcggctttcg
    23881 ggatctcagt tgtatgagat tttgtgtatc cttggttcat gtgttttttg cacaataacc
    23941 attggttacc gacttttttc tgaataccta gtttatgaag taatttattc atctgttgtg
    24001 gagacatccc ataatctgct gcaatctgac taattgttac tgaatctgta gaagataata
    24061 tgctatctaa ataggagatt ttaggttcgt actctgcaat cttttgttct gcgattaatc
    24121 ttccagttct ttcttctttt agttgagttg ctaattgaat gattgtatct ggattaagca
    24181 aagcttcttc tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca
    24241 cttcacttgt tacccaacgt ttaaattttt tggcagaggg aagttttgat tttaagatta
    24301 aactgtacaa accagattcg ttgatgattg tcatctctct tgattgacct gaggtcgtga
    24361 ttcgcgaccc catcttatct tccaaatcta catgccgcga caaagcatct ttagtgtttg
    24421 aataacccaa aacactcgca acgtctttgc ctacaaaata tggttcatca tttactaaaa
    24481 ttgtccgaac ttcgttttgt tcgaaattaa aaatttgcgg tgtgttcata tttctcattc
    24541 ctttctttta tattcgtaaa caaatttaac aacttttttc taaattcaat tgacagtatc
    24601 tgagtaatac tctatactaa gagcataatt aaataagaca taaaaacatt gattttaaag
    24661 ctttcttggc ggtctgcatt tacatatcaa tagtgttttt tgttgtcttt ttagttgtta
    24721 aatctgttta caagatatag tatagctcat tactcagaat tagtcaaatt atttctgcgt
    24781 atttttctaa actttttttg taaacattta gaaaggttgt tttatcgatg aatacctatg
    24841 aaataataaa agacttggcg aagaggaaaa agatatctat acggcagtta gaaattaatt
    24901 ttggatattc taacggttat ctagcaaagt ggaaaaataa tactcctaat tcagatgaat
    24961 taagtcggat tgctgactat ttcgatgtct ctgtagacta ccttctaggt agagaagaaa
    25021 gagaaacccc taaacatgtg gatttatcag aagacgatac tgttttttct tttgatggaa
    25081 aagaaatatc taaggagaca atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa
    25141 atgaatagtt ggagtgatgg tttgtatgta tttgaaattg aaagaaatgt tgagcgaata
    25201 caatttgaaa gtagtttata tggagatgaa agagcctggt ttctactatc caaaacccag
    25261 aatcattttt ttgaatgaaa atctatacgg tgaaactgca gaagcctttc atttatctca
    25321 tgaacttgca catttcagcg cctctcattt tgaattttca gttttgtacg atacttctac
    25381 aacttttcac tcaaaatttg aaacagaagc agataaaata gctattctaa ttttattaaa
    25441 tatctatatt gagaacgaac tgactgatga atctcagttt aatctcgaaa aatttatgga
    25501 gtattattct attcagaata agctcagata cacttgttat gctgtctgcc aatgctattt
    25561 taagaaaaaa tattcttacg caaggcaata tgtatgaata catcaagaat tattaaaaaa
    25621 accgatttca gcaaaacatt aaaaaagccc gtgctgcaac acgggctctt tcctcatata
    25681 tgagcttcta caaaaaaaat catatcacag aaatgaggaa gaaaaaatgg aaaaagagat
    25741 tttaataaaa tctccaggaa aaaccttgat taaagtaact gatgattcta tttcaattat
    25801 tagaaaagga tttataaatt tagttaatca aggtattaaa ggagaaaaaa ctatcccctt
    25861 taaaaatata tcagccgtac aattaaaaaa accaggtatg agtaatggtt atatacaatt
    25921 taccttgcta ggcggaaacg aaagtcgcgg aggtatatta gcggccacga aagatgaaaa
    25981 caccgttatg tttacaaaaa aatattggaa tgaaatggaa aatttaaaaa aatatataga
    26041 gaagcaacaa tctatattag ataataattc caaagaaaca caaatttcaa gtgcagatga
    26101 aattaaaaag tataaagagt tgttagatga agggattatt aatcaagaag aattcgattt
    26161 taagaaaaaa gaattgcttg gtttataata ttcaatatca aaagagaaag aggatctatt
    26221 aatggacggt atatttggtt ttactggtat tgttttattt attataggtt taatcatgtt
    26281 aattgtaaga tttatcaaaa aaacaaataa gaaaacacct atgattctcc ttattatcgg
    26341 gattattttt actaccgtag gtttctcgct ttcctcatca aacgaggcaa atgacaataa
    26401 caaacaggaa agctcatcat ctgatactca aactactaca tctaacagca gcaataaaag
    26461 cctaactcaa tttgaagaat ttttagagac aaataatcat gactggggtg ctttcttaga
    26521 tagttattat tctattactc cagctactga gcaaaataca gcttttacta aatatattag
    26581 tggtaaaaca tatacttttg aaggaactgt tatcgaatca atgactacta gaatagccat
    26641 tatagcagac aaagagtacg atgataaaag ttggaatgat atttctgcaa cacctaaagt
    26701 ttcatatgta atttttgcga aggacttaaa caatgctgat acctttacaa agggcgataa
    26761 agtaactttt acaggagaaa ttagttcaag aggatcgaac atagaaaaat cctatgctca
    26821 atgggatatg attaatagta aagtaagtaa aaaataaatg aaccacgccc cactaagagt
    26881 tagattgtgc acaataataa aacctataca aaagagtgcc atcaggagtc acaatgcttg
    26941 taaaggaaat gacaaaatag gaggtaacta tggataatag caatgaaaaa ctaaccaaaa
    27001 aagaatatct tgaattaata gttgaaggcg gtattggagc aattccttat attggtggcg
    27061 ttgtgcagtc actttatttt gggtctaaaa acgagaagcg tttcaaacgc atcgagaaat
    27121 tctacaaaaa tttaaacgaa tcattagaaa acattcgcga tcaaattcca gaagatgttt
    27181 tcaacttaga gaacaaggac caactcattg gaatattcga agggataaat gacgaggtag
    27241 aaaaatcaaa ggcacaaaat aaaatagata tgtataaaaa tctttataag aattgtttac
    27301 ttcgaattaa caacgcttca tgggataacg aggagtatta tcttcttgta ctgaatcaat
    27361 taacatcgat tgagattcaa ttgttagcat gcttaatgag tcgtggaaac gaaaatttca
    27421 ctggaaatat cagcatgtct ggctattcac aagagctaat agatggtagc ttaaatagat
    27481 tatctgattt tggtctgtta gaaaaaacga ttagctccat agtattaggt gatgtcggca
    27541 aacaaaatat gggctacaaa atatctcatt taggaattca atttattaac tcaactctta
    27601 cttaagtttt ctttctaata tgacatctag acctatcagc aatgcaacaa taaaaaacca
    27661 gacataccaa taatcaacaa ataatctaaa ctctggaagt tcagttattc ttttaaaaat
    27721 cactttaatc ataataaaca cctctgattg gatgatctaa tacacatcca attataccat
    27781 taaaacgaca aatagcacat ccttgcgccg gcaagcaaaa aggatgtgca aaaaataata
    27841 aaacccaaac aatgggcttc tttatagttc ctattgtatc agagaaagag agctgattca
    27901 attatggcaa gattagtcaa acgtggaaat agttggcaat acgaaatttc atacaaaaaa
    27961 gatgacggaa aatacacgaa gataagaaaa tcaggattta agacaaaagg agaagcaaaa
    28021 gatgccgcca acgaattaga atataacttg aacaaaggcc ttaaagggga tcgcaaaaat
    28081 ctattattat cagattactt tgaggattgg atgcaacttt ataaagaagg aacagtatct
    28141 cctatcactt atagaaaata cgaagatacg ttaatgaaca taaagaaata tatgccagcg
    28201 gtattgattt ctgatttaga tagagtggga tatcaacgct ttttaaataa gtatgctaaa
    28261 gaccatgtaa aatctaccgt tattaagttt aataaccata ttagagcgtc gttgaaagat
    28321 gccgtagaag aaggattaat tccgtttgat ccaactagaa aagcagtaat caaaggaaaa
    28381 gattcattga agccaaaaga agataaatat ttagattatg atcaatttaa atctttaatg
    28441 aaactcgtag aagaaaacct ttctgcgcag tactcttctc ctatgctcgt gttagttgct
    28501 ggtgccactg gaatgcgatt tgctgaactt ctaggattaa catgggaaga tatcgatttc
    28561 gaagatcaaa tcatcactat taataaaaca tggaattata aattaaatga atggggaaaa
    28621 acaaaaaacg aaacttcaaa taggaaaatt tccattgata aacatacgat tgatctctta
    28681 aaaaagttta aaatcaatca aaaagaatta ttcgagaatt ttgaaatcaa aaaccctcat
    28741 aattttgttt ttttcaactt aaaaaatgga ttagtttcat caaacgccgt cagcaaatat
    28801 ttgcgcaaaa aattaaaaga attagggatt gaaaagcaat ttactttgca tggactaagg
    28861 catacacatg catctatttt actttatcaa ggagtaaata tacttagcgt atcgaaacgt
    28921 ttaggacata gcagtttaga aactacaatg tctacttatc ttcatattgt tcgagagctt
    28981 gaagatcagg ataaagaaaa aatcaatact gtgttcgata gtttatataa aaatgataac
    29041 tagtttggca catttttggc acaaatacaa aacaaacccc tgtaccgcaa aggatacagg
    29101 ggttatttat acgttcctga ctaactatta cacgtcttct gttgtgtttg ttattataag
    29161 aaatgttatc ataataggat ttctttttgt ttgtgtttta ttaaattcga ttttttttgg
    29221 cacataattt ggcacaagta cttgttcacc tatttattta gtataataaa ttccaccttt
    29281 acaaaaagaa cgtttgttcg tatactcttt tcgaggtgat ctttatgatg gaagaattta
    29341 ttagaaaaaa tattagtgat gaatatgcag atttttatga gcaaagcaac gaaaaagaca
    29401 aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat aaacaacctg
    29461 taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga tatatattag
    29521 aggtagagac taagtttaaa aatagatcgg agtaatggtt atgcttttta atgagacgca
    29581 attatggttt aaatttgatc cttcgaatag atttgtcaaa gatttttata aggtgtggga
    29641 ttcagaagtt ttctttttag caatcgaaga tagcttatta attaatctct actattctaa
    29701 taagaactac tttaaaatcc ctgctgcgaa aactagaatg aagaaagacg tatatttttt
    29761 gtttgatatc gtgactgatg tgctagacgc tcgaagcgat catcggcgtt atgactatat
    29821 aaagtatact ttcgttgatc cagaaagata caaagattaa agtaggctac ctaaaaaggt
    29881 agcccggaac ggattttatc accatactta tgaaaggaga tattttttaa gttagtatta
    29941 agattgtgta atatgatgat atctatattt tatagtatca gtgctataaa atcaaaaata
    30001 agtcactaat taactaccac tccaattata agcctttttt ctcacttttt ttcaaaaata
    30061 tggtatgctt tttaatggct tcaaatataa aagagtttaa agcgtaacac acttatgggg
    30121 cagtggtttt tggggaacgc tttaaactct tctttattat tatctcacaa tataacccaa
    30181 atgtctttct atttaaaaat caaagtaaaa cttttcaaat atacagaaat ataactatgt
    30241 gaaacatcct ttcattaatc cataaaagga tacataaaaa agccactcat ttgagtggca
    30301 atgaagaaaa gctttagctt gtataatact cttcaaaaaa ttctaacaca gaacgattca
    30361 aatggctacg ttaatgtacc ctgtaggact cgaacctacg accggacggt tatgagccgt
    30421 ctgctctgac caactgagct aagggtactg gttgttgcca cataaagcca taaacaatca
    30481 accagtagaa tgtgtggcaa caaacctgtt atcgcatagc ttggagtgtg actattatgg
    30541 gtgatagtga agatatgcga taacatcact attttatcga atgattttta tagttgtcaa
    30601 tatagttatg taatgctcct caacgaggag ctatttttat cgtttaggaa tatttaaata
    30661 ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg tttccctctg gatcattcgt
    30721 tgcccgcatc atgacataga ctttcttatt agggaagtta cgcatgttaa aagatacatg
    30781 atagccaaca tttccagaag tattataagc ttgatttaca tctgctctat aaattccatc
    30841 agctcttact cgagctaatt ctttcccagt attgtaatcc ataatgaaga tatactcgta
    30901 tttatagtta gcaatgtgcc atccagctac atgcaaattt gcattttcga tttctccaaa
    30961 ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta ggattagctg caccagctcg
    31021 tgttggatca atgacaggct tgttttcaga agttgttgga ttttcatcgg taaatccatg
    31081 agctaaatca tatgctaatt tttctttact tacgcccatt tcagaaagat aaccgtaagg
    31141 atctgtatga tcgccccaga tgttttgtgt tacccataaa tgcgatttga ttcccggttg
    31201 gttataagga gtgtccaacg ttaatggaat accatatttc attgctgaat ctctagccaa
    31261 ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc
    31321 aatctgcaca ggactgttgg cattagcata cgaaccagca ccgtactgta cataaccagg
    31381 ttgaccgact tgataaacaa ttccgccgtc tcccacaata taagcagtat aagcgctagt
    31441 ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc
    31501 atgcagtaaa atatactgat tatttgctac ttgtgagcta ccttcatttg cgcccaaatt
    31561 aaattcattg ttgatagtat aggcaaatcc gttaattgg
//
LOCUS       DAYYFD010000029        30898 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000029 DAYYFD010000000
VERSION     DAYYFD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.029"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHA25_001206"
     CDS             complement(296..1024)
                     /locus_tag="ACHA25_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762509.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHA25_001207"
     CDS             complement(1002..2576)
                     /locus_tag="ACHA25_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8762510.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHA25_001208"
     CDS             2805..3743
                     /locus_tag="ACHA25_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762511.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHA25_001209"
     CDS             3755..4666
                     /locus_tag="ACHA25_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762512.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHA25_001210"
     CDS             4690..6162
                     /locus_tag="ACHA25_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8762513.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHA25_001211"
     CDS             6213..6824
                     /locus_tag="ACHA25_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8762514.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHA25_001212"
     CDS             6854..8767
                     /locus_tag="ACHA25_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8762515.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHA25_001213"
     CDS             8895..11876
                     /locus_tag="ACHA25_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8762516.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12208..13533
                     /locus_tag="ACHA25_001214"
     CDS             12208..13533
                     /locus_tag="ACHA25_001214"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8762517.1"
                     /translation="MEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTH
                     EMRLSLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDF
                     WYLISPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREP
                     AFAHAYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGP
                     STLAASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERY
                     QVTGVVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTL
                     TNAFPNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWL
                     QAITESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13573..14505
                     /locus_tag="ACHA25_001215"
     CDS             13573..14505
                     /locus_tag="ACHA25_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8762518.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14897..16444)
                     /locus_tag="ACHA25_001216"
     CDS             complement(14897..16444)
                     /locus_tag="ACHA25_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8762519.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16546..16899)
                     /gene="tnpB"
                     /locus_tag="ACHA25_001217"
     CDS             complement(16546..16899)
                     /gene="tnpB"
                     /locus_tag="ACHA25_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8762520.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16889..17083)
                     /locus_tag="ACHA25_001218"
     CDS             complement(16889..17083)
                     /locus_tag="ACHA25_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762521.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17309..18511)
                     /locus_tag="ACHA25_001219"
     CDS             complement(17309..18511)
                     /locus_tag="ACHA25_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8762522.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18514..18708)
                     /locus_tag="ACHA25_001220"
     CDS             complement(18514..18708)
                     /locus_tag="ACHA25_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8762523.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18755..19603)
                     /locus_tag="ACHA25_001221"
     CDS             complement(18755..19603)
                     /locus_tag="ACHA25_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8762524.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19808..20158)
                     /locus_tag="ACHA25_001222"
     CDS             complement(19808..20158)
                     /locus_tag="ACHA25_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762525.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20336..21199
                     /locus_tag="ACHA25_001223"
     CDS             20336..21199
                     /locus_tag="ACHA25_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8762526.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21823..22815
                     /locus_tag="ACHA25_001224"
     CDS             21823..22815
                     /locus_tag="ACHA25_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762527.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22831..23715
                     /locus_tag="ACHA25_001225"
     CDS             22831..23715
                     /locus_tag="ACHA25_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762528.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23764..25371
                     /locus_tag="ACHA25_001226"
     CDS             23764..25371
                     /locus_tag="ACHA25_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8762529.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25372..26856
                     /locus_tag="ACHA25_001227"
     CDS             25372..26856
                     /locus_tag="ACHA25_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8762530.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27033..28025
                     /locus_tag="ACHA25_001228"
     CDS             27033..28025
                     /locus_tag="ACHA25_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8762531.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28077..28946
                     /locus_tag="ACHA25_001229"
     CDS             28077..28946
                     /locus_tag="ACHA25_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8762532.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29366..>30898)
                     /gene="guaA"
                     /locus_tag="ACHA25_001230"
     CDS             complement(29366..>30898)
                     /gene="guaA"
                     /locus_tag="ACHA25_001230"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8762533.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         9689 a         5145 c         6234 g         9830 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaatta tctcgttatc aagaagttgt ttatcgaaac
    11941 cattgcttaa aatggtcaaa aaataagctg aatgttcgtt ttaatggatt attttatcgt
    12001 ttcgaacagt tagaagaagt ctggaaaccg gtaacgaatt tccaatcatg tcctgtacag
    12061 ctcataaatg atccaaaaga aacatacgat ttatgtataa cacaccttta taaccagcat
    12121 ttgaaaaatg acggatttta tttaaaggtt actaatgaca atcaacttac gattgaatca
    12181 gcaaaccttc gagggtttca atatgctatg gaagccttat tagagagttt tttttcggta
    12241 ggagatcaat tattattacc ggaattgatt ctgaaacatg aaccatcagt gaaaattcga
    12301 ggaatcattg aagggtttta tggtatcccc tggacacatg aaatgcgcct ctctcttttt
    12361 tcatatatga aagataacca attgaatact tttatgtatg cccctaaaga tgatgaatta
    12421 ttacgtaaaa aatggcgtga actctacgat gcgcaagaat tagacaaatt taaggagctt
    12481 ttatctgctg ctgaggctag caatatcgat ttttggtact taattagtcc tggaaatgat
    12541 attgatatta cgtgtgaaga agacattcaa gttcttctga aaaagttaga acaactgatt
    12601 gagcttggcg tatttcaatt tggtctatta atggatgata ttgactatca attaaaaggt
    12661 gctgctaaac gccgttttcg agagccagca ttcgctcatg cctatttagt gaatcgagta
    12721 gaggaatatc ttagcactcg gttgtccaat catgaattag tcatttgtcc tacagaatat
    12781 gataatcgtc atggttctcg ctatttagca acgttaagtc aagaaatacc agaacaatta
    12841 ccgttctttt ggactggccc aagcacgtta gcggcgtcaa tttctacaga agagctgcag
    12901 gaaatggctt ctgtctatca acggccgatg ctgatttggg ataatattcc ggtaaatgat
    12961 tatttagagg ataaagaact attgttcatg agtccatatg agaatcgaac accgaatctt
    13021 tctaaggaga gataccaggt tacaggagta gtttcgaatc caatggctca attagaagct
    13081 tcaaagttta cgatcaatag tatggctaat tacttatgga actgtgagcg ttttgatcct
    13141 ttggaaactt ggacgtcagt cgttgaaaaa gtagttggaa gtaaactaca accagcttat
    13201 cttaccttaa ccaatgcttt tccaaatcat tacacaagca gtctgctttc agatgaagaa
    13261 ttactgctag ccaaagaccc agaatgggtc acaaaagaaa tggctgcttt agtccaagca
    13321 gtaaaatgga tccagcaaga acaacataca tctagattga gaacagaatt ggagccttgg
    13381 ttacaagcaa tcacagaaag ttggactttc tggcaagaat ggcaattaaa aaaagatcca
    13441 aaagaagcag aagaatggtt ggctaagaaa aaaacacacc gcataggacg tgatttagta
    13501 actgcccatt tagagcaaat tatcaaaagt tagatatctt aaaaaataaa aagcaagaaa
    13561 aaggagaaca aaatggtcaa gagattaatt agtgcaaact acagtgatgt caagaaaatg
    13621 acgtcaaaag agttgaagca atccatcaaa gcgagtgaag gacgggtgat tgtttgtgaa
    13681 acggttgcta atactcttcc acaatctgga gaaattacga atgcagaagt tgcgtcagca
    13741 tttggtgcag atttgattct tttaaatggt ttcgattgtt atcgtcctgc agtttttgga
    13801 ctaccaaatg tgacgattga gaaggttttt gatctacagc aaacgattga aaacccgatt
    13861 cctaaattaa aagaactagt cggtcgtcct gtgggaatta atttagaacc cattgcccct
    13921 aaaacaaata tgatgagtga gaaagtagaa atcagccaag gaagagcaag ctctgttgaa
    13981 acgatacaaa aagcagaaga actaggagtc gattttattt gctttaccgg taatccaggc
    14041 actggagtaa ccaatttaga aattactaaa tccgtaaaaa tggcaaaaaa gtatttttcg
    14101 ggattgatta ttgctgggaa aatgcatagc gccggctcag aagagccagt tgtgaataaa
    14161 gaggcgatta tttcttatgc ggaagcaggc gcagatattt tactagttcc ttcagtaggg
    14221 tctatcccag gctttttaga gcaagaccta attgaagcgg ttcggttggc aaaaaaatat
    14281 gatttgttaa cgatgtctgc aatcggtact agtcaggaaa gtgcccgacc agaaacattg
    14341 cggcaattag ccattatgaa taaaagttgt ggtgttgatt tacaacatat tggagatgct
    14401 ggatacgggg gattggcacc agcagaaaat attttcgaaa cctcggttgc aattagagga
    14461 atgcgacata ccatcaactg cgttagccgc tcgatcaatc gctaacaaaa ttaaataaaa
    14521 aatggttaaa ttgattattt aagtgggaac atacttgaga tatcagctaa cttctgtcac
    14581 tttaaaaaag ggactgtgac agaagtgaaa atagacaaaa aatatccgaa caagagacta
    14641 ggatagcaga aaaactatcg ttttgcaacg aggaccaact ggaccgtagg agcaattctc
    14701 ttattgttcg gatatttcgc ttgttcaagg acttatgtct gtagtgccct tttttggtag
    14761 gaaaaatggt agggaaccat atcattttta atgaaaatca agcatttcaa aagttgaaaa
    14821 gtaagcgccc catagaacag tacctgtacg tcaaataacc cgacctatag aatagaatcg
    14881 ggaatgtttg gcgtttttag ttacgagaaa aatgttgttg tacatccttg ttccaaggca
    14941 acagttgatc taaagtttct gctgaaagta cgggaagatt tggtatctta tcaaatagaa
    15001 actctagata ctttacagga ttcaatccgt taagttctgc tgttttatat acactcagaa
    15061 tgattcctga agttcgtgct ccttcaagac ttgtggaatg caaccaattt ttccgtccta
    15121 tcaccagctc tttgatattt cgctccgcac gattatttgt gagttctaaa cgaccatctt
    15181 caaaaatagg ttcaaaacca cttcgtagtt tacacgcata ttgaacggct gcatccaact
    15241 tggattttgc aacaggatct atcgttgcca tccactcata aaaagcaata aataatggac
    15301 gcagttcttg ttgccgacag cttaatcgct tctcaggaga aaagtctttc catttctttt
    15361 ctaggacaaa ccatttatcc aattgtttga ctgcttgagc tgcgggaata tccgtgtttt
    15421 tctttccttt tggaattgct tcgaaaaatt tccgccgaac atgcgcgaaa caatagacaa
    15481 gtcgtacact ttccacggca ttatacccgc tatagccgtc acagtgtaaa taccctgtaa
    15541 actccttgag aaaagttttc ggaacatctc cagcacgact ttcgtcgtgg tgataataaa
    15601 cgatgggatg ctgactgtct tttcctgtac aaaactgcca gtaataggta tctgttttgg
    15661 cactttcaat cacacgataa cttgtttcat ccccgtgtag aatctcttct tgattaagat
    15721 agactcttaa tcgttctcct aaagggtcta acgcgttttg cactgcttta atatgccaat
    15781 tggtgatatt atctctcgaa atatccaacc catgtgtttg tttccaatag ttctcttgcc
    15841 gataggcagg aactttctga ccaaatttca tccggatcgt ttccgccact aaactattgg
    15901 aacctaagct attagaaatc aaaggtttag gaacgatcgc ttttttaaaa gaagaaagtc
    15961 cttctttttc gcagctttta caacaatagg tatgttgata atgggtgaga caatccaaca
    16021 tagcctgatg gaaacgaatt tcttctcgta cttttgtccg tccgatatct ttcatctgtg
    16081 aaccgcaatg ttcacaaaat tgttcttcca aaggaaggag atgttctttc tctgttatag
    16141 gtaagtgttt gattttgtct gctttcgttc ctacaactcg ttgatgtcct tttactggaa
    16201 taatggtggc agctgcttct ggaacactta cagaaatttc ttcatcacta aaaagattca
    16261 attgcccatt cgtttcctca aagagagtct ctttagagcg tccaaataat ttgttggtta
    16321 aataattgac cttttcattc aatagttgga tttcagttgt aagattctga atggtttgtt
    16381 gttgtgcttg attggtttta tgggcttctt gaagcaactg aagcaacgta tctgtagttt
    16441 ccaaaatttt tcaccacctt gaatcgaact gattttgtta cagaatacca caagaaacgc
    16501 acaagaaata ggggttcaac acattttttg tgttaacccc ccagcttaaa aaactccttt
    16561 ttttcctggc aaaatagctc gaggttgatc gatacttaat ccttctaaca accatcgaac
    16621 ctgttggttc gttaaggttc gaacttcgtc tttcgtccga ggccatttta acttgccgtt
    16681 ttctatccgc ttataacaga gaagaaaacc atcaccgtcc caatagagaa gtttgaaacg
    16741 gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg taaagctcga gttgatactc
    16801 ttgttgaata agactggcaa gaccatcaat tccttttcgc aaatctgttt ttccgcacac
    16861 aatataaata tgtttggttt gaagtaaatc ataaagcatt ggacaacagt tctttcaaaa
    16921 tagcttgaag aatataattg ttaataccgt tatagatcaa aacttcagaa ccattctctc
    16981 ttttgatgcg catcgctaac ttggcagccg cgggtttacc tttttctggt gcactctttt
    17041 cgtttcctaa ttggacagga acaatctcat ttaatttata catagaaaac ctccagactt
    17101 cttgatagct caagtatatc ttgaggtgca tttgttagta aggtactgtt ctatggggcg
    17161 cttacgttga aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat
    17221 tcccactcaa tatgagtagc ctcgtaaaga agctattata tcagtgcttt ttcagcctat
    17281 tcctcatttt ggtagggaaa tggtagggtt agatttttac tgcttgattt ccattaaagt
    17341 ttgcaacttt agattttgtt gcacaggtaa atttaatgtt tcctgtcatt tgttctgcaa
    17401 ttccttcatc attacggctc catagatgag cgtagtgctt taaagtgatt tcagggctag
    17461 aatggcctaa ccgttgcgac acgaccagaa tgtctgcgtt aaactcattg attaggtagc
    17521 taacatgaga atgacgtaat ccttttcctt gtattagggg taccttagct agtttggcat
    17581 aacgctgaat aattcgttga acagttgaac gatagagtgg gaggtcagtg tagctaagaa
    17641 taaattgttc tacaccatga tttttttgaa taactttcca cttttttagc acttcaattg
    17701 tatcatcgtc cagagaaatg atccgcaagc cgctttcagt ttttgtgtaa ggtttccgaa
    17761 taaaatcttt ttgatttgtc atttccaagg tgtgatgaac cctcaacttt ttacgtttga
    17821 agtccacgtc attccaatta agtgctaatc cttcgcttac acgaacacca gtcatgtaat
    17881 atagccagat cattaaaaaa cacatatgtt cgtagaaatc atctttataa atttttgcca
    17941 aaacttcttc aaattcttct tttgtccagt aaggaacgat tgattttcct tttggaattg
    18001 cttttgttct tttggaaata ttctcattca gaaattgcag ggtgaccgca tagtctaatg
    18061 atttacgaaa cataccgtac ataagtgaac tgtatgcttg tgagtatccg ctatcgttca
    18121 ataaccaaat tcgataattt tcacaatctc gcacattgat ttcacgaagc ttcttatcac
    18181 cgaaaaattc agtaatctga atcaatccac actcacgaga actccaagta cttttttgta
    18241 cgctagcttt gtagtgtgga acgtaagact gctccataaa ttctttgtaa gttaaatcat
    18301 aattcgcaaa tccatttaca accatgtatt cattttttat acgcatgact tctttgtgtg
    18361 cttcacgagc tgatgagaat cgttttccta gagaatcttt tgttcctttc ttctggatcc
    18421 tctttccagt cacttgatct gttcctaact caactagata agaaaagcga ccatttttat
    18481 cttgatacac acaagggtat tttgttttca tagttattcc tcctgtgaaa gaggaacacc
    18541 tataatttct tcaattactt gtcggggtac taccccaact cttttattat tataatatgg
    18601 atatccctga tttaccattc ttgtttttgc ttgtcggatt attgtggtag cttgatgtgt
    18661 tttaaatcct agagcctcta aatcttttcg acaaattgta tttttcattg tataaactca
    18721 ctttcaaaga aaacactatc attgggtaat agtgttattt ctttattttt gtataatgaa
    18781 cggtactttc ttgaaaatcc atcacttgtt caggacttcc caactcattt aaagctaaag
    18841 cattgtaaaa aaatggacgt ctaactatat attcgcttgt atatagtata tcttgattcg
    18901 tggaatcata gggtttcttt accatctttt tggtgacagt tggtagtttg agattccggg
    18961 acttgaaaaa agcttttttc ttatgctctt tcaattccag ctctttatcg aaatatttgc
    19021 tgatatagcg cccgcgattt tcgatactgt ctacatcgat cttgttgatc ttaacgaaac
    19081 cctgacccca aatcgtagtt aaccggttaa atgagataaa ggggaaatca aacaagataa
    19141 tatggtagtg gattgcaccg cgttgctgct tttcccacgt ggcgagatac tttaaatttg
    19201 cttttttagt tttatagagg tcataattca gtcgtttgat aaatttttta aattcattat
    19261 ttgcatactc aatgtcttga atgttttctt tgaaagtgag agttaaaaat ttcgtttgat
    19321 tgtcaaaatt cgtgtctaca aggcgggcaa tctcaaatcg catactttga taatgtttct
    19381 gtttgcgttt taaactgtca tattgtcctt gagcggataa ttcatcatag ttttttcttt
    19441 tttcaagagt aatatcattt tcataaatcc aagctaattt ttgttttcgt tccagattta
    19501 cttcgttttc gatcgtttta cgtttgggag taatgattgc ttcataatca taaatttcaa
    19561 tatacgtggg agtttcaatg attttctgat tataagcttg caaaaatcat cactccttgt
    19621 tgcgtattcg attgtcaaag aaccagtcat tttctcctga tgttgatcta taaatcaagt
    19681 taagggggaa ggcctgcgta gcagcccttc tagcctatgg ggtgttgggc ggtcttaacg
    19741 gacagaaacg gctctgtggg cgctgccccg ccgtttcgtc ctaaccaccc aacactccat
    19801 agtatagtta gttttgtgta gtggtacctt gttttctggg aatcaaagtt gccgtatcag
    19861 cggttgccga taacccataa ctgacaaatt gtccattcga gcgtccccaa ggtacaatca
    19921 ccaaattggt aaatgtaatt atggctaaac cttgttgggt aagttcttcc ggcgtgatca
    19981 caggatgttc agatttgact ttgacactga ttttttcgta gcgaagtttt ggtaggatac
    20041 attcgtagct ccaacctaga atttcgccct ccttttgcca gcgagtgaca ttgacgactt
    20101 ggtattcttt ttcgagtgtt tcttttggta aactgagatc ggtaattttt attggcatca
    20161 caattctcct tttttatacg aaagtttata cgatttgatt aaaaaatata gatggccatc
    20221 tacgacttgt attatatacg agaacttata cgtagtcaag agattttcta aaaaaatata
    20281 tacgattttt tatacgagtg ttataatgaa aaaaatgata aggaggaatt tgaatatgtt
    20341 tggtttagta caacctaata aagtgaaggt gggccaacga atcaaagaaa taaaagacgg
    20401 aatgtctctc tcttttacag aattagggaa tcgattaggt ttaaaaaagc cgacgatcag
    20461 ttcctacgtt caaggctatg cgttggctcc taagagtgtg atcaaccaat tatctagtat
    20521 cagcggaaaa cctgtcggct ggttttattt tgggcatgtg gaagaatata tccgagagta
    20581 tttgtttctc attggtcaga aaaaaatagt ggacgattat cctgaaatca ttgagaaaat
    20641 aaagcaagaa ttttatacgg gcgagttcaa aaatcctgct tgggagaatg agctaggcta
    20701 tcctttggag gaatttatcc aagaatgttt tgtagaatat tcgagtgaaa tcatgaagga
    20761 ctatgtcgta cagattacga aagaggtact tgaagaatcc aaaacgctaa acaaactatc
    20821 atcaaaagca aaagaagaag ctgtcacgat tctttcatct gggatcattg actatatcga
    20881 gatgagtggt gagatcaagt atggagagaa agatcgcatt gcgaaaatgg tgaaagaatc
    20941 aataaaaacc tttgatgtga aacaagacct gacatttaat gattcctatt tgattggaaa
    21001 gttgatcaat attcttggta cagatgaaga tacggagctg tttctttctt ctttatcaat
    21061 gatatttact gataagcatt tttccactcg atttggtggg gaagaattag tcgaaatatt
    21121 tcaatcgatg cgaccagcgt taatcaaact ctatcgggag aaaacaccag atgaatttta
    21181 tgattggttt gagaagtaaa ataagaaaag tggcttagat tacaagttgg tgaaaaaatt
    21241 tttttcagca taacatcagt aatctataaa ggggttgttg attatactgt agaagttaag
    21301 tactcaagag attttttgta cgtaaacaaa gcaaaaaagg gtgcagtttc actagaaaat
    21361 gtactctttt ttgtgatttt tcttagttat agagtttttt tgaaacttta aaaatgagaa
    21421 aacatattga ttttaaagag tgtagacata gcttcttttg ctaaatagat taaacttact
    21481 ggtgagtggt cttgaacata cttttaaatc acattttact gagttttatt ggattttata
    21541 attcattgga aacctcgtac ttaatctgtt tttatctatc gactagaatc tgtatatatt
    21601 ccagctatca atgaggccgc agaaaagggt gcttaaaaat aatctgttgt ttaaaatatt
    21661 attatcagaa tacttagata caaaattctg gcacaaagta aaaaaaatat tgacttaaaa
    21721 aaatggatat ggtaatctat aaacgaaaat agtggtaacg tttccaaaat gcatcttcgt
    21781 taaattttta tttttttaaa cgaaaatgga aacgtttcta aaatgtattg ggaggagatt
    21841 gaattggaaa agacattggg accaggtggt gagataaggg cggtacaacg ggataaaaaa
    21901 aatatttcga agtgggaaaa attaaagaaa cagttgcggc gtgataagtg gatgtacttg
    21961 ttgctcttac caggtatcat catgacgttt atctttaagt atgtgccaat gtacggtgct
    22021 gtgattgcat ttaaagacta taatcctatg ctaggaattt tggaaagtcc ttgggtagga
    22081 ctagatcact ttagggaatt tatcagttca ccaaatttta cgcttttgct gagcaacaca
    22141 ttaaaaatta gtatttatgg tttattgtta ggtttttttc caccaattat tttagcactg
    22201 agcttgaatc aaattatcaa taaaaaaata aaacagaaaa tccagttgat tctatatgcg
    22261 ccgaatttta tctcagtagt tattattgtg gggatgctgt ttttgttcct atccgcaaat
    22321 ggacctatta atgggttagt agaaaaggtt acggggcaac caattatgtt catgtctaat
    22381 ccagcttatt tcagaacgat ttatatttta tcgggaattt ggcaaggaat cggctggtca
    22441 tccattcttt acaccgcaac gctggcggga gtcagtaccg atttatatga ggcagcaaat
    22501 atcgatgggg caaatatttt acaaaagatt tggcatatcg atttgcctgc attgaggcct
    22561 gtgatggtga tcagcttcat cttggccgcc ggcggtatta tgaatgtagg atatgaaaaa
    22621 gcctacttga tgcaaacttc aatgaacatc cctgcctcag aaatcatttc cacttatgta
    22681 tacaaagtcg gcttgcaatc aggtgattat gcctactctg ctgctgttgg tctgttcaat
    22741 atgattatta atgtcgtttt attactgatt gtcaacaata taaccaagcg gctaaacgat
    22801 ggtcaaggct tgtaatgaaa ggagaaaatt atgaatagtg ctttatcaaa aatcgaccgt
    22861 caaatcttat ggttgaatcg agtgattgta acttttttag tcgtcatcac cattgttccg
    22921 ctgatttatg tactggtagc ttcttttatg gacccagcta ctttgatcaa taaaggtata
    22981 agcttcaatc cgaaagattg gacaatacag ggatatcaac gggtatttgc agatgattcc
    23041 attttacgcg gttttgtaaa ttcgttgttt tactctttta gcttcagctt attgactgta
    23101 tttattacga tgatcactgc gtatcctttg tctcgaccta acttggcggg gaaaaagatt
    23161 attatgactt tttttgtcat tacaatgttc gtgggtggtg gcttgatacc tacctattta
    23221 ttggtaaaga acttgggcat gttagatagt gtatgggcga ttatcgtacc aggtgctata
    23281 aatgtgtgga atattatctt agcccgtacc tattttagtg ctttgccaaa agaactttct
    23341 gaagctgcta ctatcgatgg cgccaatgac ttgcaaatac tgttcaaagt catgcttcca
    23401 ttagcgaaac caattatgtt cgtattgttt ttatatgcct ttgttgggca atggaactca
    23461 tactttgatg ccatgattta catcaaagat cccgacttac agccgttgca actcgtatta
    23521 agaaatattt tgatccaaaa ccaaccttca caagacatgg tgggagccaa tactgccatg
    23581 gcagaaatga aacaaattgc tgagttgatc aaatacgcaa cgatcgtcat ttctagtctg
    23641 ccattaatca taatgtatcc tttcttccaa aaatattttg ataaaggtgt tttagttggt
    23701 tctgttaaag ggtaagtatt gttcactatt tgtatgaata aaaattataa aggagatttt
    23761 aagatgaaag caaaatggat gctgccaatc gtttttactt ccgtactaat tttgggggct
    23821 tgtggaggct caaaagaaaa gagtaaatca agccctgatt atgaactaga aaatgtctct
    23881 tttccattga aagaaaaagt atctttaaaa atgatgtctc aaagtgcccc tttggctccc
    23941 aatgatccta atgacaaatt gattttccaa cgattagaag aagagaccaa tctccatatt
    24001 gattggacaa actacagtag tgattttgcc gaaaaaagaa atttggatat cgctagtggt
    24061 gatttaccag atgccatctt caacgccgct gcaggtgact atgatttatt aaactgggcg
    24121 gaaagtggcg ttattgtacc gattgaagat ttgatcgaag attatatgcc aaatctgaag
    24181 aaaatttttg atgaaaaccc cgaataccga caaatgtcta ctgcacctga tggtcacatt
    24241 tactctatgc cttggattga agaattaggc gaaggaaaag agtcgattca tacagtaaat
    24301 gggatggctt ggatcaacaa ggagtggttg gataatttgg gtcttgaaat gccgcaaacc
    24361 actgatcagt tgatgactgt gttggaggct ttcaagacgc aagatccaaa tggtaatggt
    24421 gaagcagatg aaattccttt tagctttatt aacaatggag gaaacgaaga cttaaaattt
    24481 ttaatggggg cattcggtct aggtgataat gatgaccact tagtcgttga taacgatggt
    24541 caggtacaat ttactgctaa caaagaaggg tataaagaag ccatcaaata tttcaatgaa
    24601 atgaataata aaggtcttat tgataaagag gctttcgaac aagattggaa tacgtatgtt
    24661 gccaaaggga aagaacataa gtatggcgtg tactttactt gggataaagc aaatatcacg
    24721 ggtgcagatg atagttatga cgtcttgcca gtcttagcgg gaccagatgg aacgaaacat
    24781 gttactcgta cgaatggcat gggtttctca agagatcgtt tcgtaattac tagtgcgaat
    24841 aaaaacttgg aacttacagc taggtggata gataaaatgt atgaaccgtt acagtctgtc
    24901 caaaataact ggggaaccta cggtgatgaa gcacaggcga atatttttga attaaaggat
    24961 ggtatgttaa aacatttgcc tttagagggg acagcaccgg gggaactaag acaaaaaacc
    25021 gaagctggag gaccattagc agttcttgat tcttattacg gcacagtcac tactatgcca
    25081 gatgatgcga agtggcgttt agatttgatg catgacacgt atttgccata tattacagaa
    25141 gacaatatct atccacgtgt ctttttagaa aaagaagaaa cagatcgatt agcaaaaatc
    25201 gaaacggata tgtgggatta catctatcgc aaaagagcag agtggatcac cggcggtaaa
    25261 atcgatgaag aatgggaaga ataccttgct gaattggaac gtgtaggttt ggaagaatgg
    25321 attcaaataa aacaaactgg ctatgataat tttttgaagg gagcacaata agtgttagta
    25381 gaagaaacgg cgattttgga taaagcaaat cgatttattg aaatgatgcg aggaaaagtt
    25441 aaccctcagt atcgacaaaa ctatcatttt tccgcaccca tcgggtggat aaatgacccc
    25501 aatgggttta tatattacaa agggcattac catctcttct atcaatacta tccttacgat
    25561 agtcgatggg ggcccatgca ttgggggcat gcgcgcagta gtgatttggt ccattgggaa
    25621 gatctaccag tagcgttagc cccagatcaa ccttatgaca gcggcgggtg tttctcagga
    25681 agtgcaattg aaagagacgg aaaactatat ttattttata caggctgtca agaaattgat
    25741 ggtattagcc atcagcatca atgtttggcg gtctctgaag atggtattca ttttagcaaa
    25801 tacagtggta atcctgtggt agcagaaaaa cagctaggtg aaaatgggct aatacaagaa
    25861 tttcgtgatc ctaaagtaat tgttcacaac gatggttact atatggtagt agctactaaa
    25921 tctacacagt ctactggtag aatagtgatg tttcaatcgt cagatttgta tacttggcac
    25981 ttttactctg tgctgctaga agggacttca gagcaaggaa taatgtggga atgtcctgat
    26041 ttattccatt tagacggacg agatgtctta atcatgtctc cgatacaaat caagaagaac
    26101 ggactggaat atcataatat cagttctacc atggcttgta ttggtgaggt aaactgggaa
    26161 acaggtcaga tgatagttga taatttccat gaaatggatt ttggaatgga tttttatgca
    26221 ccacaaacaa caagagatcc taatggtgga cgggtgatga ttgcttggat gcagatgtgg
    26281 gatcgtacat tgcctacaca tgagttaggc catctttggg caggctctat gagtttgccc
    26341 cgagagttaa gagtgaaaaa aaatcgattg gtacaaaaac ccatctcaac tgtatatagt
    26401 catttagttt atcaacaagg cagtgaaaat attgcagtga ctgacacatc agtcgtattt
    26461 cgaaacgcgc tgtctgattc ttcatattta catctagtcg cagatttgga gaaagctgaa
    26521 gtttttacaa ttgaattact gcggtctaag acggattgtc tcaaactagc ctatgacact
    26581 gctacacagg tttttgaatt ggatcgtcag aaactaggaa gagagcttac aggggcggaa
    26641 gaagaccctc tgaatgtacg aaaagttgaa gtacctttga ttgataatca gctcgttctt
    26701 gagattttta gagatacctc atcaattgaa gtctttatta atgggttaga agttatgacg
    26761 gctactttct atgagctgga aaaagctcga gatatcttgt ttcattcgat cggagaaagt
    26821 cgtattcaag cttttgaaac agcgagtgtg aaataaatgt ttgattgctt aggcgatgaa
    26881 tcaattattt aataggtttt gaaggataat gtttttagaa aagaacagat agctagattt
    26941 cccatggatt tgctattagt attttgaaaa aattagtaat ttatgacaaa attcattata
    27001 ctaaacttgt gcagaaatgt gagggatcaa ctatgaaagc aacaatgaaa gatgtcgcaa
    27061 aattagcagg tgttggagta ggaactgttt cccgggtatt gaataacggt tctgttaaag
    27121 cttctactag agaaaaagtt gaagcagcaa tgacagagtt aaactatcaa ccagattttt
    27181 atgcccgggg gttaaagatg aagcatacga acacggttgc tttgattcta cccacggtat
    27241 ggcatccatt ttttggcgaa tttgcctacc atgtagaaac agcattagaa agacaaaact
    27301 acaagcttta tttgtgtaac tccagtggta atagtgaaaa agaacaagaa tatatccaga
    27361 tggtcagcca aaataaagta gacggcatca ttggaattac ttattcagat atcgatgaat
    27421 acatttcgag taatctgcct tttgttagta ttgaccgtta tttcactgaa gaggtgattt
    27481 atgtgaccgc tgacaatgcg gccggtggac gtttggctgc aaatgaactt tatcgccgag
    27541 gatgtcagaa tgtagcatat atcggtgggt accaagaaac accgaatgaa acgaagagtc
    27601 gtcgaaaata ttttgaaatc gagtgccgtc aactggggat ttcctataaa gcgttagata
    27661 tgttagaacc tttagaccat ctctcagaac gtgtaacaaa ttttttgtta gagaatcccg
    27721 atatcgatgg gatttttacg atcaacgatc caatggcatt agctgtcatt gatatgcttg
    27781 aaaaaatagg gaaaaacgta ccagaggatg tccaagttat cggatttgat ggtcaaaaga
    27841 tgacagaagg agatccttat ctagtctcaa cgattgccca acctacaaag caaatggcgg
    27901 aagtggcagt ggcaaaatta attgctgtca ttaatggaga gacagtagaa aaacgtaccg
    27961 tattgccgat tgaatttggc gaaggtgaca caacgaaacc tttagtgaga aacgattttg
    28021 cttagtgcaa atacgtttct ttttttgaaa atgagtgaat gaaaggtgta gataaaatgt
    28081 tatatggtgg aatagaagcc ggtggaacaa agtttgtctg tgggattggt aatgaacaat
    28141 tacaagtgat agaacggaaa agttttccta ccacaacacc agaagaaacg atggagcaag
    28201 tgtttgattt ctttgataag tacaaaggaa atatcgcatc aattggtgta ggagcatttg
    28261 ggcctattga tattcaaatc gaatcaagaa cttacggata tatcaccaac acaccgaagt
    28321 tagcttggca aaattatcat tttgtaggag ctttggaaaa cgccttgtcg gttcccatct
    28381 attggactac cgatgtaaat gctgcttgtt atggagaata tatggcaggg tatggaaaag
    28441 gtaaaaatag tgttctgtat tacaccgttg gtacaggtgt tggtggtggt gctgtagtgg
    28501 atgggaaaat agtcgcaggt tttagtcatc ctgaaatggg acatatgctc ttacaacgtc
    28561 ataatgaaga tcaatttgaa ggaatctgtc cttttcatca tgattgtttg gaagggttag
    28621 catcaggacc tgcgattgaa aaacgctttg gaagaaaagc ccagactatc tcatttgatc
    28681 atccttattg gacgattgag gcagattatc ttgctcagtg tgtgtataac acaacgttac
    28741 tatatgcacc ggaagtaata attttaggcg ggggtgtaat gaaacagccg caactactcg
    28801 agttgattcg ccaatcgttt aaagaaaaaa tgggttccta cgtggatacg cctactctcg
    28861 aagaatatct actaactcca aggcttgctg acaatgcggg cttgattggc tgcctagctt
    28921 tagcaataaa gaaactcaat cattagaata gtatttatta gaactattca taacataccg
    28981 gaagtttaaa agggtattct gaaacttaaa tagtctcaat tcattcttct ttcttgagaa
    29041 tctgagtaga gacttttatt ctgtttcaaa ttaatataat tacgaaatga tattgagtac
    29101 aagaaagttc tttttaccag atcttttaaa tcgaaaaatc tgatatatat atttatagta
    29161 ctaaaaatct gattagataa aaagctaatt caataggaaa ataaaggtag tgaataaaag
    29221 caaagccctt gttagtagtt aatctaacaa gggctttggt agggaaaatg gtaggaaacc
    29281 atcttatttt ttacgaaaat caggaacttt agtaattgaa taatgttgat ttataggtat
    29341 tcctcatcca tattatgttg gctctttatt cccactccac tgttgcaggt ggtttgctgg
    29401 taatatcata cacgatacgg ttaacgtgtg cgacttcatt tacgatacgg acactgattt
    29461 tttgcaagac ttcccaaggg atacgggcaa agtcagcggt catgccgtca atagaagtga
    29521 cagcacggat acctacagtg tagtcatacg tacggccatc tcccataacc ccaacagagc
    29581 gaatgcctgg taggacggtg aagtattgcc agatgtcgcg atctaaaccg gcattggcga
    29641 tttcttcgcg caagatagcg tctgattcac ggacgatttc taatttttct tctgtcaatt
    29701 caccaaggac acgaatacct aagcctggtc cagggaatgg ttggcgccaa acgatgctgt
    29761 caggcatgcc taattgggta cctaattcac gtacttcgtc tttgaacaat gtgttcaatg
    29821 gctcgatcag cttgaattgc atgtcttcag gtaatccacc tacattgtga tgtgatttga
    29881 tggtttgggc agtttcagtt cctgattcga tcacatcggt ataaagggtt ccttgtgcaa
    29941 ggaaagaaac gccttcttca ccggcaagtt tggttgcttc atcatcgaag acatatacaa
    30001 attcatttcc aatgatttta cgtttttctt ctggatcaga cacgccagca agttttgata
    30061 agaaacgttc tttggcatcg acttttataa tgttcaagcc aaattttccg ccaagacttt
    30121 ccataacttg ttctgcttca cctttacgca acaatacgtg atcaacgaag atacatgtta
    30181 attgatcgcc gatcgctttt tgtaaaagaa caccaacaac agaggaatct actccgcctg
    30241 aaaggcctaa taaaactttt ttgtcaccga cttgttcacg aatttttgcg atctccatgt
    30301 cgatgaagtt atccattgtc cagtcaccag tacattggca gacatctagt gtaaaatggc
    30361 gcaacaagtc atttccatat tcggaatggc gaacttctgc atggaattgg attccgtaca
    30421 ttttgcgttc tgcattttgg atcgatgcga tggggcaatc tttgctggta gcaacgacat
    30481 cgaaatcagt cggtgcttgg gtaactaggt caccatgact catccaaacg gtttgttttt
    30541 caggagtgtt ttggaaaagg gccgcttctg cagaagtgat ttccaattct gcttttccgt
    30601 attcacggtt tttggctggt ttaacttttc cgcctaattt ataagtaatc aactgcatac
    30661 cgtaacagat tcctaaaact gggatcccca aatcaaaaat cttttcatca ataccaaagg
    30721 ccttctcatc ataaacactg tttggtccgc cagaaaggat gattccttta gggttcattg
    30781 cctttacttc ttctgcggta atgcggtggc tcatcaactc tgagaaaaca ccgaactcac
    30841 gaatacggcg agtgatcagt tggttatact gactaccgta atcaagtacg ataatttt
//
LOCUS       DAYYFD010000030        29432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000030 DAYYFD010000000
VERSION     DAYYFD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.030"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(239..1183)
                     /locus_tag="ACHA25_001231"
     CDS             complement(239..1183)
                     /locus_tag="ACHA25_001231"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8762534.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1479..2423)
                     /locus_tag="ACHA25_001232"
     CDS             complement(1479..2423)
                     /locus_tag="ACHA25_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8762535.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2644..3858
                     /locus_tag="ACHA25_001233"
     CDS             2644..3858
                     /locus_tag="ACHA25_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8762536.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3880..5895
                     /locus_tag="ACHA25_001234"
     CDS             3880..5895
                     /locus_tag="ACHA25_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8762537.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5978..7351)
                     /locus_tag="ACHA25_001235"
     CDS             complement(5978..7351)
                     /locus_tag="ACHA25_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8762538.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7377..7661)
                     /locus_tag="ACHA25_001236"
     CDS             complement(7377..7661)
                     /locus_tag="ACHA25_001236"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8762539.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7663..9678)
                     /locus_tag="ACHA25_001237"
     CDS             complement(7663..9678)
                     /locus_tag="ACHA25_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8762540.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9857..10162)
                     /locus_tag="ACHA25_001238"
     CDS             complement(9857..10162)
                     /locus_tag="ACHA25_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8762541.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10251..11282)
                     /locus_tag="ACHA25_001239"
     CDS             complement(10251..11282)
                     /locus_tag="ACHA25_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8762542.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11489..12364
                     /locus_tag="ACHA25_001240"
     CDS             11489..12364
                     /locus_tag="ACHA25_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8762543.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12516..13358)
                     /locus_tag="ACHA25_001241"
     CDS             complement(12516..13358)
                     /locus_tag="ACHA25_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8762544.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13450..15327)
                     /locus_tag="ACHA25_001242"
     CDS             complement(13450..15327)
                     /locus_tag="ACHA25_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8762545.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15324..16745)
                     /locus_tag="ACHA25_001243"
     CDS             complement(15324..16745)
                     /locus_tag="ACHA25_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8762546.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16748..20137)
                     /locus_tag="ACHA25_001244"
     CDS             complement(16748..20137)
                     /locus_tag="ACHA25_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8762547.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20394..21785
                     /locus_tag="ACHA25_001245"
     CDS             20394..21785
                     /locus_tag="ACHA25_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762548.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21858..22505)
                     /locus_tag="ACHA25_001246"
     CDS             complement(21858..22505)
                     /locus_tag="ACHA25_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8762549.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22513..23199)
                     /locus_tag="ACHA25_001247"
     CDS             complement(22513..23199)
                     /locus_tag="ACHA25_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762550.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23205..24278)
                     /locus_tag="ACHA25_001248"
     CDS             complement(23205..24278)
                     /locus_tag="ACHA25_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762551.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24500..25174
                     /locus_tag="ACHA25_001249"
     CDS             24500..25174
                     /locus_tag="ACHA25_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8762552.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25179..26519
                     /locus_tag="ACHA25_001250"
     CDS             25179..26519
                     /locus_tag="ACHA25_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8762553.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26668..27654)
                     /locus_tag="ACHA25_001251"
     CDS             complement(26668..27654)
                     /locus_tag="ACHA25_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8762554.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28378..29409
                     /locus_tag="ACHA25_001252"
     CDS             28378..29409
                     /locus_tag="ACHA25_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8762555.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         8703 a         5523 c         5296 g         9910 t
ORIGIN      
        1 tgtatttgcc gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc
       61 gcttattgta atcaacagat taaatttcat atgttcatat gtacggatac attttcggac
      121 cgattaattt taaccattct atttatttgc ttacctatta gatatcaata aaaaactgct
      181 taacagctat ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt
      241 ataatacagt gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt
      301 cttcttcgtt gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg
      361 gcacacctgt aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca
      421 atacagcttg ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac
      481 catagtaagt tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag
      541 caatcacatc taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg
      601 gtttaccacc gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta
      661 agatatagcc gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga
      721 aacgagttgt atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt
      781 cgttccaagc gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt
      841 cgaatcctga tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa
      901 ctaaatctaa acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat
      961 cagcatcttt acattcttca tagccacctg accatacagc cacattttca tcgccccaag
     1021 ccataccatc taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca
     1081 atactaattc attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa
     1141 aacctgtacc gacgattact actttacgac ttgttttttt catgaataaa cagctccttt
     1201 tcataactat ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg
     1261 ttaattataa tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac
     1321 agataaatga caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact
     1381 gaaaataaat ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa
     1441 caattttcct tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca
     1501 ttgggagtac atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat
     1561 ccgatacgat tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca
     1621 aagtcgaatt ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat
     1681 ttattactaa gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa
     1741 gaagtaaaat gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca
     1801 tctgctgatg aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat
     1861 gtatagagat ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata
     1921 ttttcaatca aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta
     1981 tgttcccctt tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt
     2041 gccaaatgat tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa
     2101 atatcctctt gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg
     2161 tcactttctt ccatctctat cctttctcca ttaataattt ttaaacatgt accgctatac
     2221 atataattga tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt
     2281 ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc
     2341 tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt
     2401 tcgattgatt cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc
     2461 tcctttttgt aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa
     2521 agctattttt ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg
     2581 ctattgagag aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg
     2641 aacatgctta tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg
     2701 acttctgctg gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt
     2761 tctttatctg ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt
     2821 ttgattagta gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta
     2881 gtgattgcat cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta
     2941 gcttcaagag ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg
     3001 attgctgcct tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt
     3061 caaggatttg gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg
     3121 caatatacct ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt
     3181 gttcctacac ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta
     3241 atcaaaacga atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta
     3301 tataatgctg tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa
     3361 actaatgcag cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt
     3421 tttggctggc ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg
     3481 ggaatcggct ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg
     3541 atcctaacag gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc
     3601 caagcgccca agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta
     3661 ggtactttca ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa
     3721 acacttgctg ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt
     3781 ctagtagtac acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa
     3841 caattgacaa caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa
     3901 gatagatggc tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc
     3961 caacgggaag aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg
     4021 aacgtcaagt ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc
     4081 caaggagttg ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact
     4141 tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg
     4201 atgtttatcg agggagacga agtaaaatca gatattggct ggacagcaag taattttatt
     4261 gaagaatatc cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa
     4321 atctattatc aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc
     4381 ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat
     4441 atcgaatcta tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat
     4501 tgctactata agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc
     4561 tatttgttta taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt
     4621 ttatctcttt cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg
     4681 tcagtatcag aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa
     4741 cgagatcgct ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt
     4801 ttttttgacg aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc
     4861 aagcagcata tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac
     4921 cactatatga tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca
     4981 ttaatgagat acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa
     5041 gattggatat ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa
     5101 gtcttattac tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca
     5161 tatggagaga acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa
     5221 caaaacctat ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa
     5281 tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct
     5341 aacaaagacc aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag
     5401 attactactc catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg
     5461 aatatcgtct accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta
     5521 actttctggg aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga
     5581 gatccttatg gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga
     5641 cgctatttta tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct
     5701 aatctagatt catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg
     5761 cttgaaaaaa caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa
     5821 atgaatggta aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca
     5881 aaaataaatg cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt
     5941 ctgatattgt ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa
     6001 tgtttgttct ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga
     6061 tgattccaac tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg
     6121 gtactagata atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg
     6181 gaaggataaa taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg
     6241 cccctcttct accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat
     6301 gtcctactac cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata
     6361 gtgttcctac aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa
     6421 aaacgatagg aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt
     6481 ggaatgctgg aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc
     6541 cagctgtgaa tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta
     6601 gttctctttc aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca
     6661 tcaagagcat gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta
     6721 tgtcctctgt agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat
     6781 aaccgatatt tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa
     6841 acggttgaca aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata
     6901 agatcatcca caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag
     6961 aaacataaag cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa
     7021 gtaaaatatt cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt
     7081 gcaaagcaat cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg
     7141 tttggaacat cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca
     7201 gtactaggaa tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct
     7261 tctgaagaac gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga
     7321 ttgacacaaa caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat
     7381 tcttttgaca agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc
     7441 aaaggtacga gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg
     7501 aagaaataat cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac
     7561 tcgtccacat ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa
     7621 cttgttccta atccactttg acatactgca aaaattttca tatcattcgt ctccttttct
     7681 tatgatcgct aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt
     7741 attagaaagt agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc
     7801 agctaaacag atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg
     7861 ttgaagtttc agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg
     7921 taatgcgatg tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat
     7981 agctttgata taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt
     8041 gattgcttct tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa
     8101 ttcagatagc ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt
     8161 taatacttga tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag
     8221 cgccgacaac aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg
     8281 tacttgattg atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag
     8341 agactttttc ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt
     8401 ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa
     8461 taattgctga agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat
     8521 cacggctctt atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa
     8581 tagtatagtg aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa
     8641 cggaagtaat gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc
     8701 gatcatcaca ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg
     8761 ataaaagaca ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc
     8821 catgcgatgg atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac
     8881 atctccttca acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg
     8941 ttcggaatag ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc
     9001 caacagttca gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa
     9061 actcaaataa taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga
     9121 aatcgtttta tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt
     9181 taaatacgtg agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt
     9241 tgcttctctt cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata
     9301 cgacagctga caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac
     9361 tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt
     9421 cgaataaacc atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa
     9481 tcgagtaata tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat
     9541 acgggtatca tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt
     9601 tacttcgatg atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata
     9661 actaattttg tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct
     9721 tttaaatcga ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa
     9781 cgatttctat cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg
     9841 aattgatttc gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt
     9901 tttctttgac atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag
     9961 gctttgtttc ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt
    10021 caccagagtt tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc
    10081 ctgctaaagc cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt
    10141 tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta
    10201 caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt
    10261 ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt
    10321 tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag
    10381 acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca
    10441 tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca
    10501 ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc
    10561 cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt
    10621 atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct
    10681 ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga
    10741 acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca
    10801 tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata
    10861 tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt
    10921 ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct
    10981 gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt
    11041 gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc
    11101 tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc
    11161 cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt
    11221 gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc
    11281 atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat
    11341 ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg
    11401 aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat
    11461 cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga
    11521 gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc
    11581 agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat
    11641 tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg
    11701 ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc
    11761 aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat
    11821 aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa
    11881 caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt
    11941 ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc
    12001 ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg
    12061 tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt
    12121 gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat
    12181 atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga
    12241 ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg
    12301 caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac
    12361 ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata
    12421 tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac
    12481 gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac
    12541 gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca
    12601 tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca
    12661 tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta
    12721 caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc
    12781 ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata
    12841 tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt
    12901 cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct
    12961 tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa
    13021 tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta
    13081 tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct
    13141 tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca
    13201 ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc
    13261 agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc
    13321 atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact
    13381 gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt
    13441 gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag
    13501 aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt
    13561 acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga
    13621 ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt
    13681 cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact
    13741 tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga
    13801 tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc
    13861 tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc
    13921 agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc
    13981 ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa
    14041 tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa
    14101 tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata
    14161 taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt
    14221 atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa
    14281 tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt
    14341 caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg
    14401 tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc
    14461 attatcagga gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat
    14521 caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg
    14581 accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt
    14641 gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc
    14701 tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc
    14761 attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt
    14821 ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc
    14881 ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt
    14941 ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac
    15001 tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt
    15061 atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa
    15121 tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg
    15181 taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac
    15241 tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat
    15301 aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac
    15361 cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg
    15421 tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg
    15481 tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt
    15541 aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg
    15601 gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt
    15661 gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg
    15721 aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta
    15781 ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg
    15841 ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg
    15901 gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg
    15961 tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt
    16021 aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga
    16081 tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc
    16141 gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt
    16201 tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca
    16261 ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca
    16321 atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg
    16381 ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac
    16441 gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga
    16501 tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac
    16561 gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt
    16621 gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc
    16681 tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt
    16741 ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac
    16801 aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg
    16861 gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca
    16921 gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag
    16981 taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag
    17041 ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt
    17101 ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt
    17161 cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc
    17221 atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga
    17281 tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga
    17341 ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt
    17401 ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc
    17461 ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa
    17521 cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt
    17581 ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc
    17641 ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca
    17701 atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa
    17761 tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat
    17821 ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat
    17881 ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg
    17941 cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat
    18001 ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt
    18061 taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa
    18121 tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact
    18181 ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt
    18241 cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg
    18301 gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt
    18361 cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag
    18421 tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga
    18481 atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag
    18541 cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat
    18601 cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga
    18661 gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt
    18721 ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata
    18781 gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat
    18841 cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct
    18901 gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc
    18961 catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac
    19021 catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca
    19081 tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat
    19141 tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct
    19201 ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga
    19261 gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca
    19321 atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg
    19381 ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta
    19441 taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc
    19501 ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag
    19561 gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg
    19621 ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta
    19681 ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat
    19741 cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat
    19801 cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat
    19861 aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa
    19921 tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt
    19981 tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat
    20041 ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat attatcagca
    20101 tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt
    20161 tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt
    20221 attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac
    20281 caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa
    20341 gggttgaggg agaaaaacat atgatcaggt tgttggaagg aaactataga aagatggaaa
    20401 aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac
    20461 gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg
    20521 gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg
    20581 taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat
    20641 taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca
    20701 tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt
    20761 tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt
    20821 tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc
    20881 caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt
    20941 ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca
    21001 tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga
    21061 taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca
    21121 gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga
    21181 aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat
    21241 ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt
    21301 ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg
    21361 cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat
    21421 tttattccac tctactgaat cagaatctga tttattattt atctagctat atcttttgga
    21481 tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca
    21541 gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg
    21601 tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc
    21661 ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata
    21721 tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt
    21781 tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata
    21841 cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag
    21901 tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt
    21961 acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa
    22021 tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct
    22081 ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag
    22141 atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa
    22201 tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt
    22261 ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc
    22321 gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa
    22381 aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa
    22441 gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt
    22501 gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg
    22561 ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt
    22621 ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc
    22681 caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc
    22741 aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga
    22801 ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt
    22861 aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct
    22921 ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc
    22981 agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga
    23041 aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc
    23101 ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa
    23161 tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct
    23221 gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca
    23281 atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg
    23341 cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc
    23401 ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt
    23461 gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg
    23521 accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg
    23581 gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc
    23641 gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt
    23701 tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac
    23761 gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa
    23821 ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga
    23881 ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt
    23941 aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg
    24001 attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct
    24061 cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct
    24121 tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct
    24181 gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt
    24241 aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag
    24301 attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac
    24361 ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca
    24421 tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc
    24481 aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc
    24541 caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag
    24601 tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat
    24661 gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc
    24721 agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg
    24781 tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc
    24841 attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag
    24901 ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa
    24961 agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca
    25021 gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta
    25081 tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt
    25141 acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa
    25201 agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt
    25261 tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat
    25321 tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag
    25381 gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat
    25441 taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg
    25501 agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg
    25561 atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg
    25621 ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca
    25681 tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta
    25741 tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta
    25801 gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg
    25861 cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta
    25921 ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga
    25981 cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc
    26041 agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac
    26101 catttcaaga gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat
    26161 tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg
    26221 cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag
    26281 aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga
    26341 tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc
    26401 ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca
    26461 attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat
    26521 gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat
    26581 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac
    26641 ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac
    26701 atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac
    26761 atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac
    26821 atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt
    26881 tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac
    26941 cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt
    27001 tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc
    27061 tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc
    27121 aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa
    27181 gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc
    27241 cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg
    27301 ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg
    27361 atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt
    27421 atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt
    27481 ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt
    27541 ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa
    27601 tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat
    27661 tcctcctagc aaatagattt atgattggta tattgttact tataaattta ttctatagga
    27721 gacgattgtt ttctacaaat atttagattg gaatttgatt aaaacaattt aattgaaatt
    27781 tgaaaacatt tatgttaatg aaagtcatat tctaataaaa tgtcatttgt ttctatatga
    27841 atttttatga aaaatttcat ataatttcca ctcgttgtga tggtttaatt tatccaatcc
    27901 atcaataagc aaacaactta tataaaaaag gattaattct caatataatt gctcctacca
    27961 tagaataaat cttcatgtaa agtaatgaag taccaactat ttctaatact tctttaagaa
    28021 ttatcccaac aatatcgtta gacaaaccat tattgaataa atggagagcc aatcgattct
    28081 gaaaaaatgt tttctaattt ttcggaaata aaagaaagag cctcctgcca ttagaacgaa
    28141 ccaaatccat ttcattgctc atgaagtaac cacatagcta aaaagaggat aaatcaatta
    28201 aaagatcaaa agaatgactg cactaataaa ataactaaaa atgtatcttc ttacttcata
    28261 tcttttagct agtacttagt taaaaatttg cttcctaaga tcaagacaaa ctgtctatct
    28321 tcatttgatg aaatgcggta aaatatagaa aatgaacact attggaggga atttgtaatg
    28381 gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc aacgtacaca cttgctccct
    28441 tccgtctttc aacagttttt agaagaaaat cgttcttccc tttcattagg cagtcgctat
    28501 gaatacgcaa aagattttca tctgtttact gattatttga gaaaagtatt ttctgaagaa
    28561 gtggatgatc agctgattat ccatttagaa aaacaagatt atcagaattt cttagctaaa
    28621 atctatcgac atactcgttc gttccaaact acagcgaacc aagaaacgga gcaattgttt
    28681 gaaaatagct attacgggat ttcaaggaag caatattcat taaatcattt tttacgattt
    28741 ttgtatcaaa aaggactgat tgaagaagag atttctcttt tgggtacaag tttaccggaa
    28801 acaaccaaag cagctccgcg atatatcgat gcagaacttt tcaaagattt tgagcatttg
    28861 tttatcccga ttgaaggctt tcaaacaagc agagaagcta gctttgaaga aaagaataga
    28921 ccgaggaacc ttgcaattac tgccattctt ctgtattgcg gattaaagat ccatgaagta
    28981 gtagaactta aacgtaaaga tgtatctttg accaagcaga ttcttaatct gaatagaaaa
    29041 gaaaacagac tgaataaagt accaatacca aaagaagcga tgccatttct ggagaactat
    29101 tttaagttaa cagcacaaaa tacggacgaa aattcttttg tttttcgttc ctcacatgat
    29161 cagcaaattt ctcctcggac tattcgccaa attttgagca aaattacagt atataaaaac
    29221 gtatcgccag aactatgcag aaaaacatat cgttattatg cagaactata tttagatgat
    29281 gctgacgcgg taaattattt atgtggtaat cgatacctct catcccattc ttttcaagat
    29341 atctgggaaa agatggttga tttttcttat cacgaattag cggcattcgt tcgggtttct
    29401 tccatataaa acaaatgtat gatctttttt tc
//
LOCUS       DAYYFD010000031        29100 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000031 DAYYFD010000000
VERSION     DAYYFD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.031"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHA25_001253"
     CDS             complement(<1..1031)
                     /locus_tag="ACHA25_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8762556.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHA25_001254"
     CDS             complement(1113..1802)
                     /locus_tag="ACHA25_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8762557.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHA25_001255"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHA25_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8762558.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHA25_001256"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHA25_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8762559.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHA25_001257"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHA25_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8762560.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHA25_001258"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHA25_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8762561.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHA25_001259"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHA25_001259"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8762562.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHA25_001260"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHA25_001260"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8762563.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHA25_001261"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHA25_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHA25_001262"
     CDS             complement(7636..8523)
                     /locus_tag="ACHA25_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8762564.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHA25_001263"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHA25_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8762565.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHA25_001264"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHA25_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8762566.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHA25_001265"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHA25_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8762567.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHA25_001266"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHA25_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8762568.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHA25_001267"
     CDS             complement(10454..10957)
                     /locus_tag="ACHA25_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8762569.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHA25_001268"
     CDS             11554..12519
                     /locus_tag="ACHA25_001268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8762570.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHA25_001269"
     CDS             complement(12618..13790)
                     /locus_tag="ACHA25_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762571.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHA25_001270"
     CDS             complement(13777..14922)
                     /locus_tag="ACHA25_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762572.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHA25_001271"
     CDS             complement(14936..15868)
                     /locus_tag="ACHA25_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762573.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHA25_001272"
     CDS             15985..17064
                     /locus_tag="ACHA25_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8762574.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHA25_001273"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHA25_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHA25_001274"
     CDS             complement(17789..18016)
                     /locus_tag="ACHA25_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8762575.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHA25_001275"
     CDS             complement(18119..20707)
                     /locus_tag="ACHA25_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8762576.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHA25_001276"
     CDS             complement(20727..21386)
                     /locus_tag="ACHA25_001276"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8762577.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHA25_001277"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHA25_001277"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8762578.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHA25_001278"
     CDS             complement(22159..23025)
                     /locus_tag="ACHA25_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8762579.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHA25_001279"
     CDS             23134..23775
                     /locus_tag="ACHA25_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8762580.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHA25_001280"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHA25_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8762581.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHA25_001281"
     CDS             complement(25216..26115)
                     /locus_tag="ACHA25_001281"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8762582.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHA25_001282"
     CDS             complement(26117..26914)
                     /locus_tag="ACHA25_001282"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8762583.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHA25_001283"
     CDS             complement(26892..27596)
                     /locus_tag="ACHA25_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8762584.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHA25_001284"
     CDS             27727..28302
                     /locus_tag="ACHA25_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8762585.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHA25_001285"
     CDS             28420..29097
                     /locus_tag="ACHA25_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8762586.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6166 c         5230 g         9140 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aatgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAYYFD010000032        28024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000032 DAYYFD010000000
VERSION     DAYYFD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.032"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(198..938)
                     /locus_tag="ACHA25_001286"
     CDS             complement(198..938)
                     /locus_tag="ACHA25_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762587.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1068..2015)
                     /locus_tag="ACHA25_001287"
     CDS             complement(1068..2015)
                     /locus_tag="ACHA25_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8762588.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2144..2908)
                     /locus_tag="ACHA25_001288"
     CDS             complement(2144..2908)
                     /locus_tag="ACHA25_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8762589.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(2912..3301)
                     /locus_tag="ACHA25_001289"
     CDS             complement(2912..3301)
                     /locus_tag="ACHA25_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762590.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3436..3978
                     /locus_tag="ACHA25_001290"
     CDS             3436..3978
                     /locus_tag="ACHA25_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8762591.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4126..4758
                     /locus_tag="ACHA25_001291"
     CDS             4126..4758
                     /locus_tag="ACHA25_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762592.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4892..5017)
                     /locus_tag="ACHA25_001292"
     CDS             complement(4892..5017)
                     /locus_tag="ACHA25_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8762593.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5151..6044)
                     /locus_tag="ACHA25_001293"
     CDS             complement(5151..6044)
                     /locus_tag="ACHA25_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8762594.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6226..7152)
                     /locus_tag="ACHA25_001294"
     CDS             complement(6226..7152)
                     /locus_tag="ACHA25_001294"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8762595.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7237..7998)
                     /gene="pflA"
                     /locus_tag="ACHA25_001295"
     CDS             complement(7237..7998)
                     /gene="pflA"
                     /locus_tag="ACHA25_001295"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8762596.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8242..10473)
                     /gene="pflB"
                     /locus_tag="ACHA25_001296"
     CDS             complement(8242..10473)
                     /gene="pflB"
                     /locus_tag="ACHA25_001296"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8762597.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10750..13200)
                     /gene="parC"
                     /locus_tag="ACHA25_001297"
     CDS             complement(10750..13200)
                     /gene="parC"
                     /locus_tag="ACHA25_001297"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8762598.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13212..15257)
                     /gene="parE"
                     /locus_tag="ACHA25_001298"
     CDS             complement(13212..15257)
                     /gene="parE"
                     /locus_tag="ACHA25_001298"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8762599.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15423..15857)
                     /locus_tag="ACHA25_001299"
     CDS             complement(15423..15857)
                     /locus_tag="ACHA25_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8762600.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15984..16622
                     /gene="plsY"
                     /locus_tag="ACHA25_001300"
     CDS             15984..16622
                     /gene="plsY"
                     /locus_tag="ACHA25_001300"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8762601.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16754..17629)
                     /locus_tag="ACHA25_001301"
     CDS             complement(16754..17629)
                     /locus_tag="ACHA25_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8762602.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17711..18502)
                     /gene="codY"
                     /locus_tag="ACHA25_001302"
     CDS             complement(17711..18502)
                     /gene="codY"
                     /locus_tag="ACHA25_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8762603.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18520..19920)
                     /gene="hslU"
                     /locus_tag="ACHA25_001303"
     CDS             complement(18520..19920)
                     /gene="hslU"
                     /locus_tag="ACHA25_001303"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8762604.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19934..20482)
                     /gene="hslV"
                     /locus_tag="ACHA25_001304"
     CDS             complement(19934..20482)
                     /gene="hslV"
                     /locus_tag="ACHA25_001304"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8762605.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20519..21424)
                     /gene="xerC"
                     /locus_tag="ACHA25_001305"
     CDS             complement(20519..21424)
                     /gene="xerC"
                     /locus_tag="ACHA25_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8762606.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21622..22998)
                     /gene="trmFO"
                     /locus_tag="ACHA25_001306"
     CDS             complement(21622..22998)
                     /gene="trmFO"
                     /locus_tag="ACHA25_001306"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8762607.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22967..25045)
                     /gene="topA"
                     /locus_tag="ACHA25_001307"
     CDS             complement(22967..25045)
                     /gene="topA"
                     /locus_tag="ACHA25_001307"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8762608.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25167..26024)
                     /gene="dprA"
                     /locus_tag="ACHA25_001308"
     CDS             complement(25167..26024)
                     /gene="dprA"
                     /locus_tag="ACHA25_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8762609.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26080..26847)
                     /locus_tag="ACHA25_001309"
     CDS             complement(26080..26847)
                     /locus_tag="ACHA25_001309"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8762610.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26840..27700)
                     /gene="ylqF"
                     /locus_tag="ACHA25_001310"
     CDS             complement(26840..27700)
                     /gene="ylqF"
                     /locus_tag="ACHA25_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8762611.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         7452 a         5969 c         4800 g         9803 t
ORIGIN      
        1 aactattttt gcaaacgatc tgcgaaaata gtttgttttt ttattttgcc attattttct
       61 tacttattgg gagaaaaaat tcgcttaaac tcaaaaaaat ttgaggatac gatataaact
      121 ttcggataga gtataaaata aggaaacgcc gcagcgtttc ctttccaact gtagccgaat
      181 ataaatttgt atatcactta tttaaaatag ctattttctc ccaaagagtt tccttgatca
      241 tcgatcaatc cttgtaatga gcgttctaag ataccatggc tttttttcga ttttttttct
      301 tctgactgct ttgtttcctc taacgttttt gttcgattga atgttcgtac agacggctca
      361 ccatttcttt tttcttgata cggcgtcggt tctgtatcga acaacagata agagtcatcc
      421 gtttttttca tcgattcgat taaatgatcc tcagaaattt tgctttctct agggtctggg
      481 ataatcgatg cgggtacata tttaggaaca aaataagaac gctcccgcac cttcgtgtca
      541 acagatgcgt ttcgtgtagt atgcttggac tctgtccttg tttcactttg tctgacagaa
      601 cgagaggaat caccaaaaag tttttttcgt cctgtcgatg tgatttctga ttggtaatgg
      661 ccggtataat ccggtaattt tcctttatgc ttttccaaat tttccttacg cggagctgac
      721 ttcaatccat ttgttgcacg acgcttgcga cggattttct cggtgttcgt gttcgtgaaa
      781 tgaaattctt tcatctgttc aaacggggac tgattctgtt cagtcaaaag atcttcttgt
      841 ccgtcaaata atactcgctt cttgtcgttg gttaatttta cgcctaactc atcatcgtac
      901 gcaggaaaac ggaaatgttt tcgtctgatt tctttcattc aaaccatcct tctcaaaaat
      961 ggaacttttc tcaataatag cacagattca ttgttttcag tagcaatgcg ctgattattt
     1021 taagaaaata gcgatgaatt tcataaatat atatatagta ataagactta attcgttgtc
     1081 tctctgataa ctagttcagg aaaaaaacga cgataacctt gactcgcccc tttttcttct
     1141 atttttttta acaacatttc tccacatact tcacccatac gtataatatc ttgcttgatc
     1201 gttgtcaatt taggtgaact gatttggtct aaaaataccc catcaaatcc aatcacacca
     1261 aattcttcgg gcaagtgtcc tccagcattt ataatccctc ttactgctcc tagtgctagc
     1321 cgatctgaac tacatacaaa acaagtttta tctgtaaaat ctgcccagtt ttctgtcata
     1381 tatctttcta cttctgtcga atgatttgga aaccgcaaga ttttactagg aagatcaact
     1441 tccttcatca cagataaata accagtttcc cttgaaattt caaaaggctc gtccacttcg
     1501 ataccgatat aaataatcgt ttgataatct ctttctaagg catattgcgt tgcttttgct
     1561 gtaccatatt cattatcgct gtctacataa tcatatcctt cagaattttc cccaaacaag
     1621 acaactggtt tttccaaacg gtcgatccaa tcgaagtctt tttttctgac acctgtgatg
     1681 atgtatccat cacaagaacc gacgtcgaag gcatcatttg taactaattg gagagaataa
     1741 tgcgctttac cgatccgttt agcgattccc atcagcaaat tcatgtaata gggctcagtt
     1801 gtgtcgattt cttccagtat aaataatttg atgatctgcg accgattcgt gacaagtgct
     1861 tttgcagcaa tatttggacg gtaatccagt tcagccattg ctgagaaaac taactctttc
     1921 aattcgtcag tcacctgttc cggatgattg atgactcgag agaccgtcat ttttgaaaca
     1981 ttggcacgtt ttgcaacatc tgctaaagtg ggcatgctct cttcctttct aacatgttgt
     2041 cattcttact atcaattata ccaaattttc ctaacagagt ttattatagc ccaaaataaa
     2101 aaacggaaaa tcctctatcg tcatgagtga ttttccgttt caattattta tactttttta
     2161 tcaaattttt tgctaaaaaa gtggaagctt cttccattag ttgtatttgt accatatgtc
     2221 gctgtgtttt tgaggaacga aaaacaattt cttttccgta agacttatgc cgattttttt
     2281 ctacaaatcg ttccgtttgt atataaggga ttttttcgtc agcttgtcca tgccaaaaga
     2341 acagaggacg tccagctaac ttttccggct gcaatgacaa atcatagctc ttaacccatg
     2401 aaatcaaatc aaaatagtct tttggtacga cataattata ttccgatgcg cgccttacga
     2461 tttcttttcc atattccaaa ggtgcagggg aacccatcaa acacacgccc gctactatat
     2521 gaggatgttt agccaacaag gcgcatgttg tcattccccc catcgaaacc ccgccaactg
     2581 caagctgatc atgtagaaga cctctttttt ctaaatgctt gataagtgtg tcaaattcaa
     2641 acagatttcc gtagatacta tcccaaaatg tataggatgg aatagaagaa accggctgtt
     2701 tgcgctcccc atgattcatc gcatctggca ggatcacacg aatgccctgc tgcgcaattt
     2761 ttcttccttg cgtcaaaact aactccttac ttgtctgcca tccgtgataa tagatgacaa
     2821 cgggaatacg ttcctctctt aattctttca gaacaacttc taacactgga atatgatgga
     2881 cagaacgttt caatataatt ggattcaatt gttactcacc tattttcatt aaatttcctg
     2941 atcgccatag caaccatgct ccaacagttg tctggaacag gttcagtgta ccaaataata
     3001 taaatgcagg actgaatggc tcgggaaata agcacagaga acaaagtccc accactccta
     3061 atgctagcga aaggagtgga taaatagaaa aattcaaaaa actggcaacg ctaccgaaaa
     3121 tattaccaat aaacagtatc aaaaagaaaa aaacgcttaa aattaaacca tctgtcagcg
     3181 acatctgaaa aacaatgttc tctacctcaa ttggctgttg aatcgaatag acacctaagt
     3241 atatactgag aaggagactt agcacgtggc tggcacaaaa cacacgattg accgttttca
     3301 ttttaatcac ctaacctatc tttataattc tattataatc attttatcat ttttttgtat
     3361 gtataaagtt tcaatttttc ttcacttacg attttttttg atattgttgt tatataaatg
     3421 aaaggagttt tacaaatgat tgatccaaaa atgttgggga tgatcttcct cattaatttt
     3481 gcttatatca ctctgaacac gttacgtttc atgttgacaa tgaaaggcta tcgactgatt
     3541 gctccacttg tcagtatggt cgagatcacg atttatatcg tcggcttatc ccttgtttta
     3601 gatcgtttag ataatccact gaatttattt gtctatgcgt taggttacgc agtaggtatc
     3661 agtgtcggaa ttttgattga agacaaattg gcattaggat acatcatggt cacaacgatt
     3721 ttgccagcaa atagcgcaga agataaaagt ttgccgggta tactgagaga acacggttat
     3781 ggtgtgacac agacttctgc aatggggctg gaaggtgagc ggctagttct cgaaatactt
     3841 tctcctagaa aaagcgaacg tgaactgtat cgcctgatca aagaaatcga agagcgtgct
     3901 tttatcattt cttatgagcc aaaatatatt tcaggtggtt tctggactaa aaaagtgaga
     3961 aaacgacaag gaaaataact cacaaactta aaaactgtct aatatctaaa ttaaatttta
     4021 aatgttagac agtttttatt tattattttg attaaaaaac atcgacatat actcatcaat
     4081 ttgttattat actttatata cttatcaatt taatttggag atattatgga aattcaacgt
     4141 agaaccacta attttttaca tttaggctct ttttttgcaa ctttgacgat cttatttttt
     4201 ctatgttatt cataccttaa gacctcacat gcgccttcta gtgcattagg ctggttgctt
     4261 ttggatcatt ctgatatcac agaagccgaa gaaaatccgt tttttttgat tttgtcttct
     4321 ctttgcggct tactgttttc tgtttatttc ggtgcctatt tttttcatcg acatcctggt
     4381 acgttaaacg aagcttcaag cgtattgatt gcatttaaaa cgttttcttt tttagcaatt
     4441 cttttttatt cgtttaatca caatacgctt ttcttcttta tcatgaatgg tttagtcgtg
     4501 attttatcca tttttactta ttttattttt tcactcattt ttagccaatt gaaatgtccg
     4561 ctcttctttc gattagttcc tgtcacctgt tttatttaca gcttgttttc cttttttgtc
     4621 cttgctttaa tgccactgac caacccttcg acgatccaag tcgcaggaaa tctgttggat
     4681 atgctcttca tcgtcagtct aggtgtattt atgtggcaaa gaaaaaacaa acaaaataaa
     4741 aagcattttg aagcctgact attacgactt cataaatgct tttttagttt taaacacttc
     4801 aatctaaaag aataacccaa gattggataa attattaatg actcgccatt cgtttcttag
     4861 tgattctgga ttatcaaatg gacttcctaa tctaatcagc tagttcttcc cataaaatgc
     4921 catacttgac tgaatcataa tattgattat ttaaaaatct aacttttctt atcactactt
     4981 cctttgtcat tccacatttc tcaccatttt tttgcattcc tttgtttcca gaccatgtag
     5041 tgaatctaat aggtggtaga taggcaaaat actcaaataa ttcttttaac cataaaggaa
     5101 gagaaaggct cctcctgcaa gagtcagtat aatttttgct tactgctctc ttaggtttta
     5161 ctcactaaaa ctgttccata ttctttgata taagaaaatg catgcggtat aaatttcttg
     5221 ctcagctcag aaaagatttg tcgtttaaga aaggccattt cctccattgt caatgaagga
     5281 tccaatgttg gtaattcaat ctcaaaattg tttttttcga caataggtaa ttctaatgtg
     5341 aatgattctt cttcctttat agtattcgtc acaagtagag actgatggaa aatactaggc
     5401 cggcgatttt tatatttatt cttacgtatc ctgtcaacga taacttctat ttgatcaaaa
     5461 aagtacgatt catttacgtc tccaataagt tcggctaatt ctttgaatgc cttcgttaca
     5521 ggaagctgat tcttctgtac aaaaaagtaa ttagccaaag taagcgggaa gtgttctata
     5581 gaggaaagtg tgataaaacg gtacttctca tttcctgcag ttgtcctcag caattctgtc
     5641 tctcggctgt ttcttactcc ataaaaatac gcttctttcc gttcatcttc gaaacaatac
     5701 gcccaaataa cttctcccat cgctaatttt tgttcatcaa cagagagtct cttcaattgt
     5761 tcagctatgg tttcagcagc cgaagtggct tgttgcaagt aatctttagg atcaaatatt
     5821 ggaaagttca aatgatatct tcgttcttcc ctcttgatta atcctgcatc gatcaataaa
     5881 tcaagaaaat gggaaaattt attttgcgaa aattcttttt tcaattctct tagaatcgtt
     5941 tcttgttctc gatgttcata tagataatcg gtcaatgaag aaaaaagcgg atcatcggtc
     6001 agtttttgat aaaagttttc cttatttttt gaataatagt tcatatatgt tctcttttct
     6061 atccgtttta ttttacttaa aaggctttta cgtaaaaagg aggaagaaca agagtcataa
     6121 ttgactgtta ttctcctcca tttcttcggc ctttagtatg ttctatgttt ctttttatat
     6181 caatcgctta tccattcatt ttgaatgctt catactacca tttgattagc taaatacagc
     6241 cgtcaattga ggtacgactt gttttttacg tgaaacgaca cctttaagca aagcacggtt
     6301 gttggtcaaa gttgtttgga aagcttcttc tactttttct tttcctttac cagcaacaag
     6361 taattctgaa tcactgtcta agatgtttgt gacaactaat acgaacagat cgtagccatt
     6421 tgctttcatt tcatcttcca ttgctgcttc tagctctgct tgtcgatcca tcacttcatt
     6481 aaggtccact gtattgactt gagcgatacg aacgttggaa ctgcccattg ggaaactttt
     6541 tgcatccaaa tcaagaagaa ccgcagctga tttatcactt agatttgttc ctgctttcaa
     6601 catttcaagt ccataagtgt ttagatcgac ttcagcgatt gctgcaagtt cttttgcagc
     6661 tgcttcatct tcaggcgtac atgttggtga tttgaacaac aatgtatctg aaatgatagc
     6721 tgagagcatg attcctgcga tttctttagg aattgttact tggttttctt tatatagttt
     6781 taaaataatc gtacttgtac atccaactgg ttctgcacga tagtacaatg gattagctgt
     6841 ttcaaagttt gcaatacgat ggtggtctac tacagctaaa atatttactt cgctgatatc
     6901 tgaaatactt tgttggaatt cattatgatc cacaagcatg acatctgcat tttctgcttt
     6961 agctgactct actactcgtg gtgcagatag ctgaaagtgg tctaatgcgt attgtgtttc
     7021 ttcattaggt gtacctagag caactgcttc tgtttcttta cctaattcat tttgcaagtg
     7081 tgaaaacgca atggctgccc cgattgcatc tgtatctgga ttttggtgac caaaaactaa
     7141 aatttttgac atgtcattcg ctcctttgtg atttctccta tttaataata gcaaaaaaac
     7201 ggaaagaagc aatacttatc ttctttccga tgcgacctat cgcgttaaat attttgtata
     7261 ctcttctgta tgaagcaatc gtttagcatt ttcgacacgt tcttttgtag gtggttcgat
     7321 cccttctaat ggatatttca aaccaagtgt ttcccacttg tattttccca tcgtatgata
     7381 aggaagaatc tctactctgt ctacatttga caaggtttga ataaagttgt ctagtcggat
     7441 caaatattca tcataatcac ttcgctcagg aactagtaca tggcgaatcc aaaccggttt
     7501 gttgattttt gacaaatatt gagccatttc taaaatattt tcattgtccc aatgcgttag
     7561 ttttttatgc tcttcattat cgatgtgttt gatgtcaaat aacagtaaat ctgtatactt
     7621 catcaattct tgaaaacgtg aaaagaaagg ttcttcatac gtaaaaggtt ttccgcatgt
     7681 atccaatgta gtatgcatct tccgctcttt tgctttttta aatagttcga tcaaaaaatc
     7741 gatctgcaat agaggttctc caccactaac agtaatacca cctttatctc cccagtaagg
     7801 tctgaagcgt tctgctttat ctaaaagttc atctgctgtg tattctttcc ctccgccaat
     7861 attccaagta tctggattgt gacaaaattg gcaacgcatg cggcatcctt gcataaacac
     7921 gacaaagcgc agtccaggtc cgtctactga accgaaagtt tcgatagaat gaacatagcc
     7981 gattgttttt tcttccattt aagttcacat cttttctttg atttcatttt aagtacaagt
     8041 tagctgtcga agcgaagctt tctgaacaaa atttttcgca attgccgctt cgttatgtgt
     8101 aaaatttaca gggacggaac atacaagtcc tgcccctgta aatcgtttct ttcaaataag
     8161 catactgatt attcgatttt gatgcgatgg ctattcaacg tcctttcgac gctaagtcga
     8221 aaaacttttt tcacgaactt tttacattct gtcgtgtgaa gttctcatca agacatctaa
     8281 ttgttgttct cttgtcaagt cacggaattt aactgcataa ccagataccc ggatcgtcaa
     8341 gttcggatat ttcattggat tttccattgc atcacgcaat aaatcattac tgaatacatt
     8401 gacattcaaa tgataagctc cttttgagaa gtatccatct aagacattac gcaagttttc
     8461 gattcttgta tcttgttctt ttccaagacc tgaaggattg attgtttggg tatttgaaat
     8521 tccatccaat gcacagccgt aattcatttt agctgttgag ttcaggctag ctaataaccc
     8581 actttgttcc gcattgtaac ttggatttgc tcctggcgca agtggttttc cagcttcacg
     8641 gccatcagga gtatttcctg tatttttacc gtaaacaaca tttgaagtaa tcgtcaataa
     8701 tgaagttgtt ggttttgagt tgcggtaagt tttatgacgt ttgatttgtc ccatgaagta
     8761 ttccaagatc catgaaacta attcatctgc acggtcatca tcatttccgt atttagggaa
     8821 gtcgccatcc acttcaaaat caactgctat tcctttttca ttacggattg cttttacttt
     8881 tgcatatttg atcgctgaca agctgtctgc cgcatgagag atcccagcaa tacctgtagc
     8941 aaatgtgcgt tgcaagtcag aatccatcaa agccagctga gctgcttcat aagaatattt
     9001 atcatgcata taatgaatga tgttcaacgt attgacatat agttctgcta accaatccat
     9061 catattcatg aagttatgtt tcacttcttc aaagtctagt atttctgatg tgattggacg
     9121 atattctggt ccaacttggg cgccagtcac ttcatcgact ccgccattga tggcatataa
     9181 tagtgtttta gccaagttag cacgagcgcc aaagaattgc atgtcttttc cgacaactgt
     9241 tcctgataca cagcaagcaa tcgcacaatc atcagaaccc cattgttcac gaagctgatt
     9301 atcattttcg aattgaatcg aagaactttc gatagcgatt ctgctagcat atgttctgaa
     9361 tccttctgga aggtgttcag aatacaaaac agtcaagttt ggttctggtg aaggtcccat
     9421 atttgttaat gtatgaagca gacggaaatc attctttgta actaatgaac gaccatctaa
     9481 tcccatacct gcaatggata aagtagccca gatagggaat cctgagaata attggttata
     9541 ttcaggtgta cgcgcgaatt tgaccatacg taatttcatt actaagtggt cgatcaattc
     9601 ttgtgcttct ttttctgtga ttactccatt atccaaatca cgttggatat agatatcaag
     9661 gaatgctgag atacgtccga ttgacatcgc agcaccattt tgtgatttga ttgctgctaa
     9721 atatccgaag tatagccatt ggacagcttc ttttgcattt gcagctggtt tagaaatatc
     9781 atagccatat ttcgcagcca tttctttcat ttgcgctaat gcacgatatt gttcagtgat
     9841 ttcttcacgt aaacgaatca cttcttcact cattgtatga taaccagtca tatcatgatc
     9901 ttttttcttt tgttccatca aatagtcgat tccatacaat gccacacgac gatagtcacc
     9961 gatgatacgt ccgcgaccgt aagcgtctgg aagccctgta atgattttgt ttttacgagc
    10021 agcacgcatt tcagcagtat aagcgtcaaa tacaccttga ttatgtgttt tgcgccagtc
    10081 agtgaaaata tgtgtcaatt catcgtttgg tgtatagcca ttgcttttta gcgcattgtt
    10141 agccatattg attccgccga acggcatgaa tgcttgtttc aaaggtttgt ctgtctgtag
    10201 accgacgatt ttttccaaat ctttatccaa atagcctgca tcgtgtgaag taatagttga
    10261 tacgatttct gtgtccatat catagacacc gttacgctcg aattggatat catttaactt
    10321 ttgcaattca tcccataatt tttctgttgc ttctgttggg ccttccaaaa agctgtcatc
    10381 accgcgatat tcagtgtagt tattttgaat aaagtctcta gtgtctacgc cttctttcca
    10441 tttggtacct ttaaatccat tccattgctc cattgtatgg acctcctaaa tttctgtttt
    10501 atgtaacagt ttttacgtga ttagtataac acagcaccct atctttgcaa gggttttctt
    10561 gttttttatt taacaatttt tatgttttat ttacatatat ttaaatcaac gttgacatac
    10621 caacatttaa gcattgtgaa cactttcatc tttttttgca aacgtataag gatttattaa
    10681 aaatgaatga aaaagagacg ccacctgtat taatacagat ggcgtctctt cattaaaata
    10741 ctacaacagt tacgactcga tgaaaatctc aggagcatcg ataacttcga tgacttctcc
    10801 accttttttc tcatccatcg caaatgatcc attcgatgtt cgatcaccta tagggaaatc
    10861 agttgggtca atcgttaact gctgcccttt ttgattcatc aaggttattt cttttgatct
    10921 tccttccgac atatagataa tacggtgcgg attttttttc agctcacgca tgaccgtcac
    10981 tccccgcttc gcacgtgaag tttggctgat ttcttgagca agcatgcgtt taacagctcc
    11041 tcgctgagta acgattacga tcggcgtatc accttctgct aacacaagca gcccgttgat
    11101 aatcctatct ccatctttca aattcataga cttcactcca gctgcctttg ctccaacaac
    11161 gggaacatct gttaaaggat agcgcaaacc cattccttga tgcgttacta agaaaacatc
    11221 taagcctggc tgatcttctt tcagatacac agcaatcagt tcatcttctt ccgcctttag
    11281 cttcatacag ctgagcggac ggctcttata ggtgcgccat ggttcgaact ctgtcattcg
    11341 tgtttgcttg atcaatccat tcttgctgat gaatacaaat gttttctctg cttgaatttt
    11401 ttgatatggg aaaacagcta aaatcgattc gtctacagac agattggaga ttgtttgaga
    11461 aatgtgctca ccagcatctt tccatttcaa atcgggtagt tcatggacag ggcggtagat
    11521 cacatttgct ttatttgtga tcagaagaac atggtccaat gtactcaatt cacctgagta
    11581 tagcaagtag tcgccttctc gcatcccgat ttcttctggt ttggaagcac tataagaacg
    11641 aaggcttgtt cgcttcacat agccttctcg cgtcacactg acaattacgt cttcttgtgc
    11701 cactaagact tgcgtatcaa ttttgatttc ttcgatttcg tcttcgattg tcgttagacg
    11761 agcggaagaa taattttttt tcacttctcg caattctttt ttcataacag agaaaagctc
    11821 tttgtcattt gaaaggattt tattcaactc tgtgatttgc gcaatcaagc tttcggattc
    11881 cttttgaagc tctgtgatat ccgtgttcgt caaacggtat aattgcaatg tgacaattgc
    11941 ttctgcttgt tcttctgtaa attgaaagac gtcgactaaa ttctttttgg cgtctttttt
    12001 gtctttactt tcacgaatcg ttgcgattac ttcatccaga atcgataaag ctttcatcaa
    12061 tccttctacg atatgctgtc ttttttgagc tttggctaaa tcgaattgac tacgttttgt
    12121 tatgacttgc tttcgatgct cgatataact tcttaaaatg tctttcagtc caacttgatg
    12181 cggagtcata tggtcgatag cgaccatatt gaaattgtaa ttgatctgta gctcagtgtt
    12241 tttgaacaaa taattcaaaa ttccttgagc attggcgtct tttttcaatt cgacgacaat
    12301 ttgcagacct gttctatcac tttcatctcg tacttcagct attccatcga ttttcttatt
    12361 caaacggatc tcatccattt ttttcaccaa ggtcgcttta tttacttcat atgggatttc
    12421 tgtgatcacg atttgctgtt tattaccctt cattgcctct atctttgttt tcgaacgaag
    12481 aataactttt cctcgtcctg tttcataagc ttttttgatt tcatcttttc cttgcaagat
    12541 ccctcctgtt ggaaagtcag gtccaggaat atattccatc aatttttcaa ggctcgcttg
    12601 tggatgatcg atcatataga ttgtgccatc gatcacttca gctaagttat gagtcgggat
    12661 ttctgttgca tatccagcag atatacctgt cgatccattg acgagaaggt ttgggtatcg
    12721 agctggaagg actgttggtt ctttttctgt atcatcaaaa ttccacacga gatcgactgt
    12781 ttctttctcg atatctgcta gcatctctcc acttaacttg gataatctgg cttcagtata
    12841 acgcatcgca gcaggtggat caccgtccat actcccgtta ttcccgtgca tttcgatcag
    12901 tacttcccgc aacttccagt cttggctcat gcggaccatc gcttcataaa tactgatgtc
    12961 accatgagga tgataattcc ccatgatatt tccgacagat tttgctgatt tacggaatcc
    13021 tttgtcatac gtattgccat ctttgttcat ggcaaataat atccggcgtt gcactggctt
    13081 caatccatca cggatatcag gcagtgcacg ttcttggata atatatttgg aataacgtcc
    13141 gaagcggtca cccatcactt cttctaaagt taattcttgt acttcttgtc gattttccat
    13201 caggtgacct cctattctac ttcaaataac tgattgtttt catttttatc tgctcgttct
    13261 tcatctaata aatcattaga aactgaagga gagatttcag tatcctcttt tttatctaag
    13321 atactgccat cttcttcgag cgtaaattgc acgtgatttt cgatccattt tcttcgaggt
    13381 tctactttgt cacccatcag tgtggttact cgacgttcag cttgcgcagc atcatcgata
    13441 cggacgcgga tcaacgtacg ggaagtcgga tccatcgttg tttcccacaa ttgttccgca
    13501 ttcatctctc caagtccttt atatcgctgc agcatatatc ctttgccgac ttttttaatc
    13561 attgctgcca attcgtcatc tgtccacgca tattcaatga cttgcttctt tccttgtcct
    13621 ttggatactt tatataaagg cggtaacgcg atatatactt tcccagcttc aatcagcggt
    13681 ttcatataac gataaaagaa agtaagcagt aatacttgaa tatgggcgcc gtctgtatcc
    13741 gcatcggtca tgatgattac tttgtcgtaa ttacagtctt cgatagaaaa ctcaggaccg
    13801 actcctgcac caatcgtata gatcatcgta ttgatttctt catttttcaa gatatcttgc
    13861 attttagctt tttcggtatt gatgactttt cctcgtaaag gtagaattgc ttggaacttt
    13921 ctgtctcttc cttgtttagc agatccgcca gcagaatccc cttcgactaa atacaattca
    13981 tttttcttag ggtttcggga ttgtgcgggt gtcaattttc ctgaaagaag agattcgcct
    14041 tttttacgct tcttcccatt tcggctttct tctctggctt tacgtgctgc ttctctcgct
    14101 tctctggctt tgattgcttt acggaccaac atctggctca tttcgctatt ttcctgcaaa
    14161 taaaagccca tttgttcact gatgacgtta tctactacgg tacgagcaac tggcgtacca
    14221 agtttttctt ttgtttgccc ttcaaattgc agaagatttt caggtacacg gatagaaagt
    14281 acagcagcta acccttctct gaaatcacta ccttccagat tcttgtcccg ttctttcagc
    14341 aaaccaactt ttctcgcata ttcattataa gatttcgtca tcgccgcttt cataccagct
    14401 tcatgggttc ccccatcttt tgtccgaacg ttattgacaa aagataaaac attttctgaa
    14461 taaccgtcat tgtattgata agcgacctct acttcgatac cttctttttc tcctgaaaaa
    14521 taaacaactg gtgtcaatgt atctttttct tcattcagat aatcaacaaa ttctttgatt
    14581 ccttcttcgt aatgaaaaat ttccttgact ggttcttctc cacgaagatc agaaagagag
    14641 attttaacgc cttttagaag aaaggcagat tctcttaacc gttcggctaa aatttcatat
    14701 gaaaatttcg ttgtactgaa tattgtatca tctggtaaaa aatggacagt cgtcccgttt
    14761 gctttttttg tttttcctat ctttttcaaa gtaccgtcag gtttaccgcc gtttttaaat
    14821 ttctgctgat actctacgcc atctctaacg atcgttacaa ttagccattt cgataaggca
    14881 tttaccacac tggctcctac accgtgcagt ccaccagaag ttttgtaccc tccttgacca
    14941 aactttccgc cagcatgcag cacagtaaaa atcacttcaa ctgttggtat tccggaagca
    15001 tgcattccta caggcatccc acgtccgcta tctgcaacag ttatactatt gtcttcatgg
    15061 atggtcacat caatctcact gccataacct gataatgctt cgtcaactgc attatcgacg
    15121 atttcataga ctaaatgatg caagcctcgg ctgtcagtgg atccaatgta catccctggt
    15181 ctttttctaa ctgcctctaa tccttccaat acttggatgg acgagtcatt gtattcgttg
    15241 tttacttttt ttgccaaggc aaacgctcct tcaataatac tttgcggcaa aaccgcacca
    15301 cgattatcat actagaaaag gtcgtgaaaa aaaagagggt agacgagaaa tcaattttaa
    15361 ttatctccta accgcttttt cgacattccc ctctatcgat catctcttct ttgtggtcat
    15421 ttttattttt ggttttcttt caacttacca agttcaatct taatacaacg atccattacg
    15481 atatctgtac gtcctgcttt tcttagcatc tcagctgctt ctttgttttc aatccctaat
    15541 tgtgcccaga acactttagc atcagttttc aaaaaatcac ctgctatttc aggtaaaaat
    15601 tcactccgtc taaatacatc tacgatatcg attggatcat cgatttctgt caaattcgca
    15661 tagactttct cgcctagaat ttcttcacca gccaataatg gatttactgg tatgatttta
    15721 tatccattgt cttgcaatac ctgtgcaacc atgtaacttg ttcgttctgg tttattgctc
    15781 aagcctacga cggcaatatt ttttgcttct tccaaatagt tgaaaatcgt ttcttgtgtt
    15841 ggatttttta cagacatttc tgccacccct tgcttttctt tttattccat ctattatact
    15901 tgtatctttc attttttcaa ttgttcctgt tacctattca tgctaaaata aaggatagtt
    15961 ttcaacttga aagagggtaa aaaatgaaat tgatcttatt acttatttta gcatatcttt
    16021 taggttctat tccttccggt gtctggattg gcaaattgtt tttcaaaaaa gatatccgac
    16081 aacacgggag cggtaatact ggaacgacca atacatttcg tgtattaggc aaaacagccg
    16141 ggattgttgt cctgcttatg gatattttaa aaggtacatt ggctacttgt ttgccgatga
    16201 tcttccacct ttctgggatt aaccctcttt ggtttggggt atgcgccatt cttggccata
    16261 catttccgat ttttgctggg ttcaaaggag gaaaagctgt tgcgacaagt gcaggaatgc
    16321 ttttaggatt taatcctgta ttttttgttt attcttgtat catctttatc gttagtttat
    16381 tttgtacaag tatggtgagt ttgagcagta tgatcagtgc agtattgatc actttatcta
    16441 cgatcgtcct tccttatgta gctcctgtga ttttggctaa gcccaattgg ttactgacga
    16501 tcattgcctt tttagtttct tcctttatct tttatcggca taaagacaat atcaagcgta
    16561 tcagaaacca tactgaaagt cgaatcccgt ttggtttgaa tagcagcaag aaaaaatcct
    16621 gatacttttt cattccaaag agataaaacg ggagtgtgac aaatcgatgt cacactccct
    16681 tattacttac aggatagctt ttttacacac cttggtaaac ggtgttccct gctaacttct
    16741 cggtcacaac ctcttacttc actgtgatgg aatattttgt cttgaagatt tcatgggcac
    16801 ctagttgatt gatccctact ttttccatca agtttccact actgttcacc gtgtcagcga
    16861 ttccgcacca cggttcgata caaacgaaag gcgcttcttt tggataagga gaccagatcc
    16921 ctacataagg catatcttta tagcttaaag ttacgctatg ggaagatttt tcacttcgaa
    16981 tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc atcttgctca aaaagttcac
    17041 gattcaaagc cagattcgta tttgtctgac ctaacgtctt ttgatccata tcgatgaatg
    17101 ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga aaatgccaga tagaaatctt
    17161 caaatgacaa tccttcttca agaggtacat tgaaagcagg atgtccaccg atagaaaaga
    17221 acatttcttt ttcaccggta tttgccactt gataatgaac cgtgatgccg tctcctccta
    17281 attcatagct gatcactaat tcaaaatcaa atggatactt ttctaatgtc tccggcgtac
    17341 tccttaagat gaaactagcc atttcttgtc catgttcaat gacttcaaaa tccatatccc
    17401 gagcaaatcc gtgttgcccc atttgatatg ttttcccttg ataaacatac tgatcatctt
    17461 ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct gccccaatac gctggatcac
    17521 cttgccagat atactctaaa ccatgttctt ttgatttcag actgatcaat tccgcaccgt
    17581 gttcagcgat ttcggcgatc agctggtcat tttgaataat aacagtcatt gccattcctc
    17641 caacttcgac ttacttttta aaatgggaag gctggaacaa acatttctat ctgccccagt
    17701 ccccttactt ctaataactg tgttttgcta actcgtcttt aaaacgggaa ttcaatactt
    17761 ttaaataagt tcccttcatg cctaacgaac gagattcaat gattccagct gattccaatt
    17821 ttcgtaaagc attgacgatc acagaacgag tgatgccgat actatcggca atattagaag
    17881 cagtcagacg accttcatct ccatccaatg cttcaaagat agcttgtaca gccttcaatt
    17941 cgctataaga taatgtgttg attgccattt ggacagccgt tgcactccgt acatctgctt
    18001 cgatgcttcg agacttctga taaaggatct gcataccgac aaccgtagct ccatattctg
    18061 ctaagactag atcgtcatct gcaaacgatt gttccatacg agataagata atcgtaccca
    18121 aacgttcgcc agctccaaag atcggaacga cagtcgtcag accaaatgga tatttctctc
    18181 ttgactctac cggaaatgca gtaatatcac tagtaattgg gatattagct tcagttttca
    18241 tcaactgtgc cactgtatca gtataagatt caggaaattg tttctcaaca aacattgttt
    18301 tgattcgttc attgttcaca tcgtgacgtt cattataacc tagtaatact ccgtcacttc
    18361 ctatgatata ggtgttgctg tcaagaatac cacctagtgt gacagccatt ttatcgtaag
    18421 gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa aagtgtgttt atcttgcggg
    18481 ttttcgttag caagtctgtc attgtcgtcc tccgattttt tataaaatat agcgacttaa
    18541 atcttcattt gccgcaattg agccaagttt gtcatccaca tatgcttctg tgatcgttat
    18601 ttctcccatt tgcatatctg gtgcttcaaa taatagatct tctagtagtt tttccaaaat
    18661 ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg ttgacatcga aagcgatttg
    18721 agcgatccgt tcaattgctt cttttgtaaa cgtaactgac acattttctg taccgatcaa
    18781 cgcgacgtat tgttttacta aagcattatt tggttcggtc agaatcttca caaaatcttc
    18841 agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt ccttgaagtt ctggaattag
    18901 atcacttggt ttagacaaat ggaaagctcc tgaagcaatg aataggacgt gatctgtttg
    18961 gatgatcccg tattttgtgt ttacttgtga accttcgaca attggcaata tgtctctttg
    19021 gacgccttct ctggatactt ctccagattg ctgtgattta gaagtgatct tatcgatttc
    19081 atcgataaaa ataattccac tttcttccgc taatttgatt gcctcactat ggatatcagc
    19141 atccttgact aattttgctg attcttcctt gatcaataac tctaaagctt ctttgactgt
    19201 taccgtccgt tccacttttt tcttcggtgt caaagcacct agagtttcat ttaggtcgat
    19261 ccccatttgc tccaatccat tgttcatcat tggtgcaggt ttcttttggt cttcaacttc
    19321 aatggttact tcacgactat ccagtaaacc cttttgtagt tgttccaaga ctgtacggcg
    19381 attgattcga atatcttctg tcacttcttc ttgcggttca gcggtatttt gctgactagc
    19441 attaaaaaga ttcatcattt gttcaaattg attagtggat tgtttctgtt cttttttgat
    19501 accaggtacc aagattttta ccaaacgttt ttctgcattt tttttagcac ttgtgtatac
    19561 acggctgtac tgctcttttt ctacgatttg gatcgcattt tctactaaat cccgaaccat
    19621 tgattctaca tctcttccga cataaccgac ttcagtaaat tttgttgctt cgactttgac
    19681 aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt tctgttttac cgacccctgt
    19741 aggtccgatc atcagcatat tttttggtgt aatatcttgt tgcatttttt catctaactg
    19801 caaacggcga tagcggttac gcaaagcaac ggcaacagat ttttttgctg cgtgctgtcc
    19861 tacgatatat tcatctaatt cacttacgat ttgtcttggt gtttgtttta attcatacat
    19921 atctactcac ctcttacagt tcttctacga taatattatg attagtaaag atacagatat
    19981 ctgcggcaat atttaacgca ttttctgcaa tttctttcgc actcatgtcg ttttgagcat
    20041 agtttttcat tgctcgggct gcagccaacg caaaattccc tcctgatccg atagctaaga
    20101 taccatcatc tggtgtgata acttctcctg tacctgaaac aagaagcatt tctttatcat
    20161 tcattacgat cagcatcgct tcaagttttt gcatagattg ctgtgtccgc cattcttgag
    20221 ctaattcgac agctgcgcgt tgtagattcc cgttatattc gtttagtttg ccttcaaatt
    20281 tttcttctaa agtaaacgca tctgcaacac ttccggcgaa acctacaaca acttctccat
    20341 tgtagatacg tcgtactttc tttgcagtgc ctttcatgac aacagattca cccattgtta
    20401 cttggccatc tccagccata gcaaatttcc cgtctttttc gacggcacag atagtggttg
    20461 aatgaaatgg tgattcgacc atgtttgttt cctcctaaat ttgttctttt ccttattttt
    20521 acgctcttgg atggaaagaa cgatagtttt tttgtaaact ttcttttgtt acatgggcat
    20581 agatctgggt agtcgataaa ttggcatgtc ctagcaattc ttggactgtc cgcaaatctg
    20641 ccccattatt taataaatga gtagcaaatg tatggcgcag catgtgagga tggatatcac
    20701 tatccaaact gctttttttg atcatttgat tcaagacata ttcaattcca gtaggcgtga
    20761 ttggttttcc atgatgattc acaaaaacga tatcatgctc ttcgtgatat tttgtcatca
    20821 atacctggcg gccttccctg aaatattctt gcagagcatc ttgagcaaaa gaacctagag
    20881 gagcatatct ctctttgttc cctttaccat ggatcaacat gacctctgaa tcaaaatcaa
    20941 tgtctgatac cgacaaactg ctacactcac ttaaacggat gcctgacccg taaaggactt
    21001 ccagtaaagc gcggttcctc aaatccaaag gactatctcc tgctacgctg tcaaaaagtg
    21061 cttgcatttc attttcataa aaaaaacgtg gcagtctcaa atttttcttt ttgagatgaa
    21121 cgtaagaaaa tggattttct tttatcactt cttctttgag aagatactga taaaacgaac
    21181 gtaggctggc tatcttgcga ctgatagaat ttctgctata gctaggacga tgatcagcta
    21241 atgcactcaa atagacacga accgtaaaat attccgcctt cagcagatca gcgtctccag
    21301 attcctccaa aaaatgtttg aaatggagaa tgtcttcttc atatgcttct ttcgtttttt
    21361 ctgaatatcc tctttcgaca atcaaataac gcaaaaattt ttgttcccac gtggcttctg
    21421 tcattttctc accttccttt tgtcccagag aataatttag cacagcgttt ataaaaagac
    21481 aaataaattg tcagaattta accaatattt ctaagtttgt tcacaattgg cgcaattcct
    21541 cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa aagagcgaga ctatccgtct
    21601 caccctcttc atggagtttc cttactcatt tgggattgca gttttttcta attctgtttt
    21661 tattgcctca ttagctttta aagctcgttg tgcaattgct tcatatcttg cttttttgtc
    21721 tcgaatgcgc tctggcagtt caggaaacag accatagttg gcattcatcg gttggaaatg
    21781 ttttccttcc gcatgagtaa tgtagtaagc catacttcca atagctgttt cttgtggaaa
    21841 ctcgactggt tccatttctt ttgctaagcg tgcggcgttg attccagcaa gtagtccgct
    21901 cgctgcactt tcaacatagc cttctacacc agtcatctga cctgcaaaga acagatcttc
    21961 gcgtttcgtt gattggtaag tcggtttcag caattctggc gaattcataa agctgtttcg
    22021 gtgcatcaca ccatagcgaa cgaattcagc attttctaac ccaggaatca tccggaaaac
    22081 acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc acgatgttgt ataaagaagc
    22141 tgcagcattg tcttgacgca gctggataac agcataagga cgtttgccag ttttaggatc
    22201 ttccaatcca actggtttca taggtccgaa aagcatcgtt ttgaatcctc gctgtgccat
    22261 tacttcaatt ggcatacatc cttcaaaata tttttctttc tcaaaagatt taagttctgc
    22321 cacttcggca ttgattagtg cctcatggaa agctttgaat tcctcttctg tcatcggaca
    22381 gttcaagtaa gctgcttctc ctttgtcata gcgggacttc aaatatactt tatccatatc
    22441 gatcgtagct ttgtctacga ttggtgcagc tgcatcatag aaataaaaac catctgaacc
    22501 attgaatgct ttgatgcttt ctgctaatgg atgagaagtc aaaggtcctg ttgctactac
    22561 ggtgattcct tccggtattt cagtaatttc ttcgtttatc actgttacta atggatgctc
    22621 tttgactttt cgggtaattg tttcagaaaa agaatcgcgg tcaacagcta aggcaccacc
    22681 agctggcact gctgtttcat ctgccgaagt gattactacc gaattcaatc ggcgcatttc
    22741 ttctttcaat aaccctactg cattagccaa actgtttcct cttaatgaat tggaacatac
    22801 tagttcagca aaattacttg tgtgatgcgc ctcagtcgat ttgactggac gcatttcata
    22861 taaacggaca gggactcctg cttgtgcgat ctgccaagca gcttcacttc cggcaagccc
    22921 tgctccaaca acatttacca ttttcaatga aagaacctct tttctattat ttttggatgt
    22981 cttcttcgta atctccattt acacagacta cttgtttacc gcctttgact ttcttctcga
    23041 ccagatatcc gtcacatttt ggacaatttc tgcccactgg tttatcccaa gaagtgaatt
    23101 cacattctgg gaaacgggaa caaccataga atagtctgtt tttcttagat tttcgttcaa
    23161 tgacttgtcc ttcatgacat actgggcaag tcacaccgat ttcttttacg attggtttcg
    23221 tattccgaca atcaggaaaa ttactgcaag cataaaactt cccatatttc cctaatttta
    23281 tcaccatcgg atgtccacag acatcacaat cgaatcctgc tggttcatct ttgatttgaa
    23341 ttttttcgat tttttcttcc gcattcgtca gttctttttc aaacggttta tagaaacgat
    23401 caaccacttt gacccacgct tccgtgcctt cttcaatttt atccaaatct ccttccattt
    23461 ctgctgtgaa atggatatcg acgatttgag ggaagaattc acagattaat gaattgacaa
    23521 tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa ttttacatag tagcgtcttt
    23581 ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc taccccgttt tcttctagtg
    23641 cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg agtaaaatgt tgttttggtt
    23701 caatatcaac agattggact acatcgcctt cttcaagttc tggaagaata ttttctttct
    23761 cttctttgcc gtcatctcta ccttccacat acacttgcat gaatcctttg aatttgattt
    23821 ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa tgtcactttc attgtatcta
    23881 aaatagctgg tgtcatctga ctagccacaa aacgtgacca gatcaatgta taaagtttca
    23941 attgatcttt atctaaatat tgtttgagtt catctggtgt acgaagcaca ctcgttggac
    24001 gcacagcttc atgggcatct tgtgcgcctt gagcattttt gatctttcgg tgatcatgtc
    24061 tagaaaattc ttttccatac gtatcttcaa taaatgctgc ggcctctgct tttgctgtat
    24121 cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa gccgactgtt ccttgtttac
    24181 ccaaagtgat tccttcatat aattgctgtg ctaccatcat ggtttttctt gttcgaaagt
    24241 ttaacttacg ggcagcttct tgttgcaggc tacttgtagt gaaaggattc gcagggtttc
    24301 ttttgcgctc tttcttttct acttttttaa cttcgaagtc tgttccatca attctagcag
    24361 tgacttcttt tacactttgt gcatctggta atttcttttt cttaccgtcg attccccaga
    24421 aatttgcttt gaatttcttg cgagcttttt taaaattacc gtcgatgctc caatattctt
    24481 ctggaacaaa ttctcggatc tctttttcac ggtcaataat aatttttaaa gcgacagatt
    24541 ggacacgtcc tgcacttaac cctttcttga ccttacgcca gagaatagga ctgatcgaat
    24601 aaccaacgat tctgtctaag gtacgacgag cttgctgtgc atctactaaa tctacatcga
    24661 tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt tgtgatttca ttgaagacca
    24721 cccgattttt ttctttcaga tccaacccaa gtaggtacgc taaatgccaa gcaatcgctt
    24781 ctccttctct atccgggtca cttgccaagt aaactttttg agcttttttt gctgcggctt
    24841 tcaggctttt gatgacatcg ccttttccgc gtatagaaat ataatggggt tcataattgt
    24901 tttcaaaatc gatacccatt ttacttttcg gtaaatctct tatatggcca acactggcca
    24961 ttactttata gtttcgtcca agatatttct caattgtctt ggcttttgca ggtgattcta
    25021 caatcactaa atatttatac gccattactg aatggactcc tttccattgt cgtgttccta
    25081 ctattaaaag aggattcata caaatcgtag ctaatcatat ccattcagaa aacgtttgtc
    25141 aagaaaagca aatgaaaagt atggtgttat tctttaaatt ctgccaagat atcttcaccc
    25201 gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac atccggtaga atgttctgtt
    25261 aaaatatttc ctggaacaga aaaaactgtt cttccaagat ccaatgcttg ctgtgctgtt
    25321 atcagagagc cgcttttttt cttggcttca aagacacatg ttcctttaga caaagctgcg
    25381 ataatccgat ttctcattgg aaaacaatgc tttttcgggc cggattgagg aggatattca
    25441 ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt cacgattttc agcaggatag
    25501 acttgctcta tccctgttcc aatgactgcg attgtccgtc caccatgaga gattgctgct
    25561 tggtgaccgt atgtatctat ccctttcgcg cagccactga cgatcacata tccagcttct
    25621 atcactggtt ttagtaaata ataaactgtt tttttggcta aagcagaagt gtgtcttcca
    25681 ccaacaacgc ttaacatgtt acaagaaagt aaagatagat caccattata aaacagtaca
    25741 attggcggat aggtcaagtg aagcagctga gggggatatt tgggatcaaa gcaagtgata
    25801 aacgactgtt tcgattggat tttgccccag ttagcgtgaa tttcctgcca acttttgcga
    25861 aacagctccc ggtgtttggt tattttacct atcttaatga tgtcttcaaa tccgatttct
    25921 ttataaggat actgcatcat ccagttagca agtgcccatt ttccttgact actgattcct
    25981 tttgtataag caagtttcaa taaattttct tctatttgat acatcataaa accgcctttc
    26041 gtctctcttt ctaataagat acgtaaaagc ggctgttttt tattgtaaat atttttttac
    26101 aggtgcaaaa cttttacgat ggattggcgt aattccttgt cgttcaagtc cttctaagtg
    26161 ttcctttgtt ccataacctg cattatgcga gaaaccatat ccaggataaa gcacgtcata
    26221 ttctttcatc aattggtctc gatatacttt agcaatgata cttgcagcag caatcgacac
    26281 agatcgtgca tcccctttga ttattttttc ctgctgaata ggcaaatcca aggtcatcgc
    26341 atcgatcaac aaatagcttg gtacaatgga caatttttct actgcttgga tcatcgctaa
    26401 tttacttgct tcataaatat tcacatcatc gattgtctgc tgatcaacga tcccgatccc
    26461 gattgccagg gctttttctt gaacttgttg ataaagaagt tctcgctttt tctctgataa
    26521 ttgtttggag tcattcagcc ctaatatgat ctcatttttc ggcaagataa ctgcagctgc
    26581 cacgacaggt ccagctaatg gtcctcttcc tacttcatca atgccggcaa tccattggtg
    26641 accttgtgcg tacccttgct tttcaaagca aaacatttct tcatattttt ccttaattaa
    26701 tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct tgctgcacgc ctttgcgtgg
    26761 atcatttctt aatgattcta gtgtaggatc atctattctc tgtatgcttt tcaatcgttc
    26821 tttgatactt tggatggatt cagtcatcac ttttttgctc catttcttcg caacgatcca
    26881 gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac gactaattcg ctgccacgat
    26941 catagtcttc tttataccca cgttttgctg taatatccat cagtaattcc ggtgccaatt
    27001 tttcttcttg ctcgattgat aaaccataac gatcgctgat tcttcctgaa taatatcggg
    27061 caaaaaagtc taatccgtaa atcgctaaat catctaaatg gacaagctgg tctttgatcg
    27121 cgcctgtcag cgctagtttt ttgccgattt cttcatcttc aaatttgggc cataaaatcc
    27181 ctggtgtgtc taacaattcc aactcattac ctaaacgaag ccactgctga ccttttgtta
    27241 cgccaggctt atttcctgtc tgtgcaattt tcttgcctac tagtcgattc atcaacgtag
    27301 atttcccaac atttggaata cctaagacca ttgcccgaat agcgcgaggc ttgatccccc
    27361 gagcagcatc tcttgcccgt ttttctttca atgcttcttt agccgcaggt actagacctt
    27421 taattccttt actttcttgt gccgtgatag ctaatgcaag atggccttgt ttacgaaaat
    27481 attcttgcca cagctttgtc tgttgtggat ctgccagatc cgctttattc aataaaacaa
    27541 ggcgaggttt ttgctgaatg atctgatcta gcattggatt acgagaagat aaaggtaatc
    27601 gtgcgtctac caattcaaat acgatgtcca catatttcat tttttcagaa acttctcgtc
    27661 gggcttttgc catatgtccc ggaaaccatt ggatggtcat tgttctctcc attcctttca
    27721 taccggaata ttccccggct ttacttgttt ttttcttttt caatataaat tcactttttt
    27781 attataaaga aattccatct attagttaag tgaaagtgaa ccagtcctca ttctatcaca
    27841 tcttcacttt tttgatgatg caaattttta ttttggtttc tatatatgaa tctaaatttt
    27901 tgacagcgtt tcctaaataa gagatgatag acgaacaaaa cagaaaggag acgaaacaaa
    27961 tgaatagtat ggtttttgtt aattttcctg ttgctgatgt caatcgttcc gttgcttttt
    28021 atga
//
LOCUS       DAYYFD010000033        27742 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000033 DAYYFD010000000
VERSION     DAYYFD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.033"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHA25_001311"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHA25_001311"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8762612.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACHA25_001312"
     CDS             complement(937..1518)
                     /locus_tag="ACHA25_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8762613.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHA25_001313"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHA25_001313"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8762614.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHA25_001314"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHA25_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8762615.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHA25_001315"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHA25_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8762616.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACHA25_001316"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACHA25_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8762617.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACHA25_001317"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACHA25_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8762618.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHA25_001318"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHA25_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8762619.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACHA25_001319"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACHA25_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8762620.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACHA25_001320"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACHA25_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8762621.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACHA25_001321"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACHA25_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8762622.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACHA25_001322"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACHA25_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8762623.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHA25_001323"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHA25_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8762624.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACHA25_001324"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACHA25_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8762625.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHA25_001325"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHA25_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8762626.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACHA25_001326"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACHA25_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8762627.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHA25_001327"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHA25_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8762628.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHA25_001328"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHA25_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8762629.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACHA25_001329"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACHA25_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8762630.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACHA25_001330"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACHA25_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8762631.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACHA25_001331"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACHA25_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8762632.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACHA25_001332"
     CDS             13972..14157
                     /locus_tag="ACHA25_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8762633.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHA25_001333"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHA25_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8762634.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACHA25_001334"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACHA25_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8762635.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACHA25_001335"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACHA25_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8762636.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHA25_001336"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHA25_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8762637.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHA25_001337"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHA25_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8762638.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACHA25_001338"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACHA25_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8762639.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACHA25_001339"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACHA25_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8762640.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACHA25_001340"
     CDS             18232..18879
                     /locus_tag="ACHA25_001340"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8762641.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACHA25_001341"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACHA25_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8762642.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHA25_001342"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHA25_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8762643.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHA25_001343"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHA25_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8762644.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHA25_001344"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHA25_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8762645.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACHA25_001345"
     CDS             20317..21255
                     /locus_tag="ACHA25_001345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8762646.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHA25_001346"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHA25_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8762647.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACHA25_001347"
     CDS             21935..22774
                     /locus_tag="ACHA25_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8762648.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACHA25_001348"
     CDS             22750..23619
                     /locus_tag="ACHA25_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8762649.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACHA25_001349"
     CDS             23609..24406
                     /locus_tag="ACHA25_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8762650.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACHA25_001350"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACHA25_001350"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8762651.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            26019..26213
                     /locus_tag="ACHA25_001351"
                     /pseudo
     CDS             26019..26213
                     /locus_tag="ACHA25_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26401..27177)
                     /locus_tag="ACHA25_001352"
     CDS             complement(26401..27177)
                     /locus_tag="ACHA25_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8762652.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            27300..27707
                     /locus_tag="ACHA25_001353"
     CDS             27300..27707
                     /locus_tag="ACHA25_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFM8762653.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT         9031 a         5067 c         5853 g         7791 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggt attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgatgaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt agcgctgaaa
    25981 atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa tatttacgcc
    26041 atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc gtttttgcag
    26101 tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa aacaatcagg
    26161 tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact tggcagttgg
    26221 aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt ctacactctt
    26281 agtaaagagg aaactgatcc ttttgtctac attcttaaag tacttaaact aagtacttta
    26341 ttttactctt taggattgat acagtagcat ctatcccttt attaataatc ttttattttt
    26401 ttaaataaag atgttgtctc catcttcagg cactaaaact ttttcggaaa ttcctttttc
    26461 tttattgaaa gactttaatt cttctcttga cagtccccaa tggtttacag cttccatatg
    26521 aacggcaata attgtggaat ctggtattgc tttatgcaat tcatataaat cgtctttccc
    26581 cataataaga gattctccaa ctaaaaaatg attatctcca gcatttgcta ctatgatttc
    26641 tggattatac tgatcaatag tttgtttaac agcttcatac caaactgtat ctcctgtaaa
    26701 gtatagtgtc ttctcagtcg aatgtttgaa aacaataccg caaacattac ctacataatt
    26761 caagatttct cctctgccat gtttgccttt tgttttaacc aattgtatag attcaaaaac
    26821 tgtgtcttct cgtagtacct ccacattttt aaaaccatat agtcgaattt cattcgcatc
    26881 attttcatct tgcacaaata attttatttc tttaggtagg acctctttcg ctatatcgtc
    26941 ccaatgatct agatgtaaat gtgttacaat aacagcatca ataccgttga taatatcttc
    27001 gactggcatt ggcaaatcaa ctagaggatt catttgatct tttcttggtg atggaaaagg
    27061 acctttcgaa ataaatggtg gaagtgttcc tttttccgct aacattggat caattaaaaa
    27121 ttttttacct ccatagtcta agatagctgt tgcattacga atgtgatgta tattcataaa
    27181 cgttgtctcc tttttgttga tatgaaatat ttttagtata attcaattcc atacaataaa
    27241 taaaccgaac aaaaaaagtc ttttactatt tttacttaat ttatgaaagg ttgtttacca
    27301 tgttagacca aacagatata aatatcattg aggaactatc aaaaaatagt cgaatcacaa
    27361 tgaaagaact tggagaaaaa gtacatttga ctggacccgc tgtctcggcg agagtgacaa
    27421 aactagagga aagtggagtt attgaaaatt atacagtaaa attaaatatg gaaaagctag
    27481 ggttttcgat tcacgctttt ttaattataa ttacgcaaaa tttaaatcat aaaccctatt
    27541 tatcatttat taaaaatcat gaaaaattct tgattagaaa ttataagatt agtggagggg
    27601 gctgttatct tttggaatgt agatttactt caaaaaaatc aatgaacagc tttttggaac
    27661 agcttaatga atatgcgaat tataaattat ctattgttat agattaataa taaaactagc
    27721 agatggtaaa aatactaata tg
//
LOCUS       DAYYFD010000034        27450 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000034 DAYYFD010000000
VERSION     DAYYFD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.034"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHA25_001354"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHA25_001354"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8762654.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHA25_001355"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHA25_001355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8762655.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHA25_001356"
     CDS             8118..9086
                     /locus_tag="ACHA25_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8762656.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHA25_001357"
     CDS             9104..10009
                     /locus_tag="ACHA25_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762657.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHA25_001358"
     CDS             10006..10812
                     /locus_tag="ACHA25_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762658.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHA25_001359"
     CDS             10969..11937
                     /locus_tag="ACHA25_001359"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8762659.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHA25_001360"
     CDS             complement(12254..12778)
                     /locus_tag="ACHA25_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8762660.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..14876
                     /gene="scm"
                     /locus_tag="ACHA25_001361"
     CDS             13065..14876
                     /gene="scm"
                     /locus_tag="ACHA25_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8762661.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15031..16017)
                     /locus_tag="ACHA25_001362"
     CDS             complement(15031..16017)
                     /locus_tag="ACHA25_001362"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8762662.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16040..16606)
                     /locus_tag="ACHA25_001363"
     CDS             complement(16040..16606)
                     /locus_tag="ACHA25_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8762663.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16620..17036)
                     /locus_tag="ACHA25_001364"
     CDS             complement(16620..17036)
                     /locus_tag="ACHA25_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8762664.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17493..19427)
                     /locus_tag="ACHA25_001365"
     CDS             complement(17493..19427)
                     /locus_tag="ACHA25_001365"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8762665.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19729..20664
                     /gene="pplA"
                     /locus_tag="ACHA25_001366"
     CDS             19729..20664
                     /gene="pplA"
                     /locus_tag="ACHA25_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8762666.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20754..21857
                     /locus_tag="ACHA25_001367"
     CDS             20754..21857
                     /locus_tag="ACHA25_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8762667.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21870..22823
                     /gene="menA"
                     /locus_tag="ACHA25_001368"
     CDS             21870..22823
                     /gene="menA"
                     /locus_tag="ACHA25_001368"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8762668.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23637..24983)
                     /gene="gor"
                     /locus_tag="ACHA25_001369"
     CDS             complement(23637..24983)
                     /gene="gor"
                     /locus_tag="ACHA25_001369"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8762669.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25117..26370)
                     /locus_tag="ACHA25_001370"
     CDS             complement(25117..26370)
                     /locus_tag="ACHA25_001370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8762670.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26376..27299)
                     /locus_tag="ACHA25_001371"
     CDS             complement(26376..27299)
                     /locus_tag="ACHA25_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8762671.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
BASE COUNT         8012 a         5819 c         5207 g         8412 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    14281 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    14341 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    14401 gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca
    14461 cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag
    14521 attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    14581 catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt
    14641 cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata
    14701 acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa
    14761 gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat
    14821 tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa
    14881 atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt
    14941 atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga
    15001 aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt
    15061 aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt
    15121 caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg
    15181 ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa
    15241 ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac
    15301 cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc
    15361 taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt
    15421 ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa
    15481 cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt
    15541 atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt
    15601 caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa
    15661 taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg
    15721 accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag
    15781 catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg
    15841 tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt
    15901 gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt
    15961 ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta gcttcataaa
    16021 aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc
    16081 cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg
    16141 ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca
    16201 acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca
    16261 tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga
    16321 ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa
    16381 tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctc
    16441 gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac
    16501 caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt
    16561 gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc
    16621 taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta
    16681 tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc
    16741 tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta
    16801 taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg
    16861 aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg
    16921 ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata
    16981 ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt
    17041 gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac
    17101 ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa
    17161 ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg
    17221 acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt
    17281 tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag
    17341 gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta
    17401 ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga
    17461 aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt
    17521 ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac
    17581 ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt
    17641 acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt tgtcgcactg
    17701 catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc aaagaatacc
    17761 atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg catgatcttt
    17821 gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta tgcataactc
    17881 aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc tccgctggca
    17941 gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac attgtattcc
    18001 catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa ccaagtactt
    18061 ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa ccaaactaaa
    18121 ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga agaatgtcct
    18181 cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata ttggaacata
    18241 taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt cattacgata
    18301 gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca tttggcacca
    18361 atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc gcctttgatc
    18421 gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc
    18481 ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat atagatgttt
    18541 ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg tccgcgtgct
    18601 gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt ccaaatcagt
    18661 gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc cacttctacg
    18721 attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc agcttttgca
    18781 gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac catcagtgtg
    18841 atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc gatcagttcc
    18901 ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag catggcttta
    18961 cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat atgttcccga
    19021 atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac accaggtgta
    19081 ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg ataggaacct
    19141 aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc tgtgacaagt
    19201 tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc ttctggttct
    19261 acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga atgacgatcg
    19321 atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt tgtagctgaa
    19381 acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga tcatccgctc
    19441 cttatagact atctatataa tgaatatatg ataattcttt tagtttgata cttattacac
    19501 aatctccata tattatatag atgttctgtt cttttgtcta actccttttg gatttttttc
    19561 aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt tgaaatatta
    19621 catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa tgtataatga
    19681 atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat gaagaaaaaa
    19741 cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg cgcttgcggc
    19801 gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca atcttcgact
    19861 gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt gcaagacggc
    19921 acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt tgaaatggtt
    19981 gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa cggcgaatca
    20041 aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac aaacccagaa
    20101 aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag cggtgtggaa
    20161 gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca acaattgatc
    20221 caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc tgatttgaaa
    20281 gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt gcaattcgaa
    20341 atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga caaagacggt
    20401 aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag cggtacagaa
    20461 ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg cgaagaagat
    20521 ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca ttcattcatc
    20581 atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac aatcgaagta
    20641 gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg gagggtggga
    20701 cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa aagatgcgga
    20761 aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta actggctgca
    20821 gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata ttgaaagatc
    20881 catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg tatgatgaag
    20941 gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt gataaaatca
    21001 cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg atcaaaccag
    21061 taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat agccaagatt
    21121 ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga atcggttttg
    21181 atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta gtggattatc
    21241 ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa ggaatgatca
    21301 tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa gtcttgaaaa
    21361 aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc ttaggccaca
    21421 gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac atggctcgcg
    21481 gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct ggaatttacg
    21541 agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca aaaacgggtt
    21601 atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca attgatggtg
    21661 acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa tacgtcgaaa
    21721 gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa gtatatgtga
    21781 ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg atgggtgacc
    21841 gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta gaagtagttg
    21901 aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta ttctctatcg
    21961 ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga atgctagttt
    22021 ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct cattctgaag
    22081 tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta ttgattcgaa
    22141 acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta acgatcaata
    22201 cgggttggct tttacttttg atgggattta tctgttgttt tattggagta ttctatacat
    22261 tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg gtgactatgg
    22321 gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat gtctatgatc
    22381 tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg gctatagcag
    22441 caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg ttggctaata
    22501 atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt ttttatatcg
    22561 gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat ttcgtgattt
    22621 taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc ttgaccttgc
    22681 caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct atcagtttca
    22741 gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg ctattactaa
    22801 caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg ccctgcatca
    22861 agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt tgggcttatt
    22921 accgcagatg caagcttttg aacaccgttt atcaggaggt tgtgaaaaag tcgcttagtt
    22981 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    23041 aataccgaga aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt
    23101 cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct
    23161 aataccgaag gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac
    23221 aatttttttc acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga
    23281 actttcttgg tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat
    23341 ttatttatac aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt
    23401 taaacatcga taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct
    23461 tcgtcagtta cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt
    23521 atcgaagcaa tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat
    23581 tttctggttt tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat
    23641 cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct
    23701 tctttagttg cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca
    23761 ataccaatag catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt
    23821 tcgcattttt ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa
    23881 actttcaatt cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt
    23941 gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct
    24001 ttctttccat taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc
    24061 agatctaatt taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg
    24121 tatttatcta ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct
    24181 aatttttcgg tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt
    24241 tcaccatttt caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata
    24301 tgatgtgcgt aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc
    24361 attttatcaa acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct
    24421 aaaccacgaa atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct
    24481 ctcttaggta actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca
    24541 ggaatggtca ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag
    24601 gtttcaccgt ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc
    24661 tcgacatgat tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt
    24721 tctcgtcttt ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa
    24781 cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta
    24841 ggtacacaac cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact
    24901 tttgcgccat gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt
    24961 acgatgtaat catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt
    25021 tatttaacga tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc
    25081 gaagatgctt aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc
    25141 tccaggtttc accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg
    25201 gtcaatggat tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa
    25261 tcctggacca taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt
    25321 agcaatctgt gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat
    25381 tttacgacgt tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt
    25441 agctaattct cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata
    25501 gttttcagga tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga
    25561 tacataatgg atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat
    25621 caattctgga atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc
    25681 cacttcgcct gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc
    25741 atacttccag cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg
    25801 gttggacaat gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc
    25861 gatttcggta tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc
    25921 taaaacgaca cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc
    25981 tgaagcttga gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat
    26041 caaagctgga tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa
    26101 ttcccctgct ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt
    26161 cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc
    26221 tgcacggctt cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg
    26281 gatcgcggtt tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa
    26341 ttttcttttt actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata
    26401 taaaagaatc ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc
    26461 attttttccg ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg
    26521 aatagtcgag tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat
    26581 gtacagtaat gatgctgata aagttcattc aattcattcg agagattcag atcattgctg
    26641 gcaaaaatat gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta
    26701 ggttccgaga taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta
    26761 tagtaattct gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg
    26821 gtttctacag gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca
    26881 cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc
    26941 gttactaatg tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc
    27001 gtataaataa tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa
    27061 gccaaatatt caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata
    27121 ttcgcttttt taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta
    27181 aatgaagcag gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct
    27241 gcagcaagca actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata
    27301 attttctcct cctacaactt cataatagta gcatagctct aaaaactttc caatacttct
    27361 tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat
    27421 attctgtcca aatataaaaa gagattgtga
//
LOCUS       DAYYFD010000035        27189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000035 DAYYFD010000000
VERSION     DAYYFD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.035"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..192
                     /locus_tag="ACHA25_001372"
     CDS             <1..192
                     /locus_tag="ACHA25_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762672.1"
                     /translation="LLIDLNMNKTQLREISGVSPNVIAKLGKNELVAMESLLKIAAAL
                     NVDVSEIISSDVIDAEDVK"
     gene            193..2910
                     /locus_tag="ACHA25_001373"
     CDS             193..2910
                     /locus_tag="ACHA25_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM8762673.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFPVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            2903..4858
                     /locus_tag="ACHA25_001374"
     CDS             2903..4858
                     /locus_tag="ACHA25_001374"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8762674.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            4848..5984
                     /locus_tag="ACHA25_001375"
     CDS             4848..5984
                     /locus_tag="ACHA25_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8762675.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(6035..6238)
                     /locus_tag="ACHA25_001376"
     CDS             complement(6035..6238)
                     /locus_tag="ACHA25_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762676.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(6254..8086)
                     /locus_tag="ACHA25_001377"
     CDS             complement(6254..8086)
                     /locus_tag="ACHA25_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8762677.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(8089..8535)
                     /locus_tag="ACHA25_001378"
     CDS             complement(8089..8535)
                     /locus_tag="ACHA25_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM8762678.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(8528..9478)
                     /locus_tag="ACHA25_001379"
     CDS             complement(8528..9478)
                     /locus_tag="ACHA25_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM8762679.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            9873..10490
                     /locus_tag="ACHA25_001380"
     CDS             9873..10490
                     /locus_tag="ACHA25_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762680.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            10587..10793
                     /locus_tag="ACHA25_001381"
     CDS             10587..10793
                     /locus_tag="ACHA25_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762681.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            10798..11112
                     /locus_tag="ACHA25_001382"
     CDS             10798..11112
                     /locus_tag="ACHA25_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM8762682.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            11113..12255
                     /locus_tag="ACHA25_001383"
     CDS             11113..12255
                     /locus_tag="ACHA25_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM8762683.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            12257..12799
                     /locus_tag="ACHA25_001384"
     CDS             12257..12799
                     /locus_tag="ACHA25_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM8762684.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            12860..14797
                     /locus_tag="ACHA25_001385"
     CDS             12860..14797
                     /locus_tag="ACHA25_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFM8762685.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            15008..15778
                     /locus_tag="ACHA25_001386"
     CDS             15008..15778
                     /locus_tag="ACHA25_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8762686.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            15771..16115
                     /locus_tag="ACHA25_001387"
     CDS             15771..16115
                     /locus_tag="ACHA25_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM8762687.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            16108..18351
                     /locus_tag="ACHA25_001388"
     CDS             16108..18351
                     /locus_tag="ACHA25_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8762688.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            18489..18698
                     /locus_tag="ACHA25_001389"
     CDS             18489..18698
                     /locus_tag="ACHA25_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762689.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            18695..18979
                     /locus_tag="ACHA25_001390"
     CDS             18695..18979
                     /locus_tag="ACHA25_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8762690.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            18979..20331
                     /locus_tag="ACHA25_001391"
     CDS             18979..20331
                     /locus_tag="ACHA25_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM8762691.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            20328..20756
                     /locus_tag="ACHA25_001392"
     CDS             20328..20756
                     /locus_tag="ACHA25_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM8762692.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            20924..21289
                     /locus_tag="ACHA25_001393"
     CDS             20924..21289
                     /locus_tag="ACHA25_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8762693.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            21423..21995
                     /locus_tag="ACHA25_001394"
     CDS             21423..21995
                     /locus_tag="ACHA25_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8762694.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            22010..22792
                     /locus_tag="ACHA25_001395"
     CDS             22010..22792
                     /locus_tag="ACHA25_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM8762695.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            22792..24027
                     /locus_tag="ACHA25_001396"
     CDS             22792..24027
                     /locus_tag="ACHA25_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM8762696.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            24101..24478
                     /locus_tag="ACHA25_001397"
     CDS             24101..24478
                     /locus_tag="ACHA25_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM8762697.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            24480..24674
                     /locus_tag="ACHA25_001398"
     CDS             24480..24674
                     /locus_tag="ACHA25_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762698.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            24658..24834
                     /locus_tag="ACHA25_001399"
     CDS             24658..24834
                     /locus_tag="ACHA25_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM8762699.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            24831..24974
                     /locus_tag="ACHA25_001400"
     CDS             24831..24974
                     /locus_tag="ACHA25_001400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762700.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            25057..26622
                     /locus_tag="ACHA25_001401"
     CDS             25057..26622
                     /locus_tag="ACHA25_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8762701.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            26678..>27189
                     /locus_tag="ACHA25_001402"
     CDS             26678..>27189
                     /locus_tag="ACHA25_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762702.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSK"
BASE COUNT         8478 a         4934 c         6196 g         7581 t
ORIGIN      
        1 ttattgattg atttgaatat gaacaaaact caacttcgtg aaatatctgg cgtcagtccc
       61 aatgtaatag caaaactcgg taaaaatgag ttggttgcaa tggagtcatt attgaaaatt
      121 gctgctgcct taaatgttga tgttagtgaa ataattagtt cagatgttat agatgcggag
      181 gatgttaaat aaatggcaaa aaataaagtt ttaaacatca ctgaaattga agatggtgta
      241 aaaaaaatac tttcaaatag gggcgatgat ttcatcactc aatttttgtc cttgtacgat
      301 atccccaaaa cgtcgattac acgtgcaaaa tcaaaattcg ataatggtga accatttgtg
      361 attaagaaca aacttcatta tgaagaaatt gaaggcaacg ttgtcattgc gattgatgcg
      421 attgctcaat caatcaaaga gcaaaaatct aaaccacgat atatcttggt aaacgattat
      481 ttagatattg ctgcaattga tacgaaaact catgaaactt tgaatattcc aattgaagag
      541 ctaccggcta atgctgactt tttcttagcg tggaatggca ttgagaaggc tgattatcaa
      601 gctgaaaatc cagctgaccg aaaagctgct gaacgttttg caaaattgta tgatgtggtg
      661 gctaaagata atcctgatgc caacgagcat gcatttaact tgttcttgat tcgagtgctg
      721 ttcttgttgt ttgcagaaga tacaggaatt atgaataagg gttcctttac aaatgttcta
      781 aaaacaagaa cggctgaaga tggttcgaat tttaacgaag taatcaaaga actctttgca
      841 atcttggata ttaatgagtt agctcgcaat ggcaaggatg agtggctact tgaatttcca
      901 tatgttaatg gaaaactgtt tggtgagcca catgtggatt tgaccttttc aaaagtttca
      961 agagagctgc ttattgaagc aggggaatta ttgaattgga atgaaatcaa tcctgatatc
     1021 cttggcgcaa tgattcaatc agttgcttca gcagaagacc gtcacgttgc cggtatgcat
     1081 tatacaagcg tgccgaatat catgaaagtc atcaagccgt tattcttaga tgatttgaca
     1141 gagtcatttg aaactttaaa gcttcgcagt gaagaaaacg agctgaagga cattactgaa
     1201 aaaacaagaa acgataataa gagaacaatc atcaatgatc taagtgcttt gttgacgcga
     1261 atttctaata ttaagttttt ggatcccgcc agtggttctg gtaattttct gatcattgct
     1321 tataaggaaa tccgtagact tgaaattaag attttgcaat tgttgggcga cttacaaaat
     1381 aatgattcca tgccactatc agtgattcat cttggtaatt ttaatgggat tgaattggat
     1441 gattttgcgc atgaagtagc aagactttca ttatggattg ctgaacatca aatgaataaa
     1501 gagcttgaag aagcaatacc aggtaccatt gctgagttgt taccgttaaa agatgctggg
     1561 aatatagtgt gtgccaattc tcttcgaatt gattggaatg aggtgttgcg actcaaagaa
     1621 aatcatgaaa tatatataat gggaaatcca ccgtatctgg gcaaaagtct tcaaaatgct
     1681 gaacaacgtg aagatatgga aaaacttgtt ggcgatattc gaaaatataa attgcttgat
     1741 tatatttttg ggtggttttg gaaagctaaa caatatattg caacatctca tggctcgttc
     1801 gcatttgtta ctactaattc aatttgtcaa ggtgaacaag ttagtatcct ttggccgtac
     1861 cttttaaaag atgaggtaag aatttcattt gcatatcaga gctttaagtg gaacaacaat
     1921 gcaaagaata atgccggtgt gactgttgtc attattggta tgacaaataa tgtgaatatt
     1981 catcctaagt tatatttaga gaatgggtca gtaatgagtg tttcaaatat taatgcatat
     2041 ttagctaatg gtgaaaatgt atttgtcaac gaatcaagaa atagtatttc aaatttaccc
     2101 tctttgaaat ttggcagtat ggcaaatgat ggtggtaatc tagtttttcc agtggcagaa
     2161 tactggaatt tgattcaaaa atatccagat attaaacatt tatttaagaa atatttagga
     2221 tcaacaggat taataaatgg agacttaaga tatactttat ggttgaccga ggaagatgca
     2281 actagataca aacaaaattc tcttattaaa gaaacaataa aaaatgtaaa aagttatagg
     2341 gaaaaaagca agagaaaaga aactcgagca ctagctgaga agccatggtc atttggtgaa
     2401 gttcgttatg atgaaaccag tgagaatagt attctaattc ctcgtgtgtc atcagaaaaa
     2461 agagattatg ttcctatggg tatagttggg agcgatacta taatttcaga ttcggctatg
     2521 gcaatctata acgctccaat gtggctgctt ggtctgttag aatcccgaat gcatatggtg
     2581 tggctgcgct ctgtcggtgg gaaattagaa actcggtacc gttattcagc cgggttagtc
     2641 tataatactt ttccgattca ggatttatcc gcccagcgta aaaatgaaat gactcgcgtg
     2701 atgacagaaa ttcttgattt acgagaatac gaaggcggca cgttagccga tttgtataat
     2761 aaagacagta tgccagaatc attacgtaag aaacaccagg aactggatgg gattgttgat
     2821 agagcttatc agcaacgccc gtttgaatct gacgaagatc gacttggaac attactaaaa
     2881 ctctatcagg agatgacaat caatgactag taatttattt gaaattaatt ttgataaaaa
     2941 tcaatcaaaa acaacaaatg acctcggtat gcgtgaaatg caagaacgtg tttatgaaaa
     3001 gcgtgcgtca cagtatttgt tggtaaaatc accaccagct tcaggaaaat cacgtgcatt
     3061 gatgtttgtt ggcttggata aactccataa tcaaggaatt aaaaaagtgg ttattgcagt
     3121 tcctgaacgc tcaattggta aatcatttaa agatactgaa ttatcgaagt ttggttttta
     3181 ttgggattgg gaagtaaagc caagtgacaa cttaactatt gggggcggtg aaaaatcaaa
     3241 ggttcaacac tttgttaaat tcatgaactc gactgaccca agtgataata tcctgattgc
     3301 cacacatgct acgttgcgat ttgcttttga agagttggac gattcattat ttgacaatac
     3361 gctattagcg attgatgagt tccatcatgt gagccgtgat gataaatctg tgttaggtaa
     3421 tgccttacgt agcatcatgt caaactctaa tgcgcatatc ttagcaatga ctggttcata
     3481 tttccgtggc gactctgttc agattctgga accagccgac gaagataaat ttgataaggt
     3541 cacttacact tattatgagc aactggaagg atataaatat ctgaaatcct ttgcaatggg
     3601 gtatagtttc tatcgtggaa aatatacaga tgctttggat aaagtaattg atgtcaacaa
     3661 aaaatcaatt atccatattc ctaatgttaa ttctggtgag tctactaaag ataaatacgc
     3721 ggaagttgat gaaatacttg atttattgac ggacggtggt aatatcacac agaacgagga
     3781 tggtatttat gaaatcgaac gtccagatgg taaaaaacta ttagtggccg accttgtgaa
     3841 tgaagaagga cgtgaaaaag tctcagcata tctagcaagt attacggatg acattaaaga
     3901 tcttgataag cttgatatca tcattgcact tggaatggca aaagaaggct tcgattggcc
     3961 atttgcggaa tatgcactga caatcggtta tcgtaactca ttaactgaag tgattcaaat
     4021 cattggtcgc gtaactcgtg acagccccaa taagactcat gcacagttta caaatcttat
     4081 tgcgcagcca gatgcacagg atgacgaagt attattcgcg gttaacagta ttatgaaggc
     4141 aatttctgca tctctactta tggaacaagt gttggcgcca gtttacaagt ttaaaccaaa
     4201 agatggtaag ccagacaaac caggtgatct ttcaatcaga ggactgaaac ctgctgatac
     4261 gacggaacgt acaaagaaaa tcatcgaaaa tgatatggaa gatttgaaat ctagcatttt
     4321 acagagccaa gatattcaaa atgcgattgt ttcaggtgct gatgcgaaga tgattaataa
     4381 gacgttgatt ccacgtgtca tcattgatcg ttatcctgat ttgacgaacg aagagttaga
     4441 aaccgttcgt caacatacag ttgccgatat aaatattgcg gctggacgga caacaacttc
     4501 agaagatggt agccgcgaaa ttattaagat ggccgatcgt ttcattaaca ttgatgaatt
     4561 gaatattgat ttaatcgatc aagtgaatcc gttccaacgt gcgtatgaag tgattagtcg
     4621 tgatattgat gcaccgactc ttcggtttat tcaggattat ttgtcatcaa ataagtatga
     4681 ttttactgac gaacaattgg ttgcggccta ccaagctgct aagaagttcc gtgcagaaaa
     4741 tggtcgaaat cctgatcgaa atagtaaaga tgatggtgaa aaatacttgg cctttgcttt
     4801 gctacgactt gctgagataa aacgtgaaag agaggcagcg aaagaagatg gcgaatagtt
     4861 tagatagcat ctttgaagat gacgcttttg atgatttaac caagcaagtt gaaaaaaaga
     4921 aagttgtaaa acaagaccca gaagttgcta aatttcagga gattatagac tttgtgaatg
     4981 aaaacggacg agagccagaa aaaactcctg aatggtctag tgaacgcgca ttgtgggctc
     5041 gtcttagcgg atttcgagga aaacccgaaa ggtctgagaa ggttaagaag tatgacactc
     5101 ttggtttatt ggagaaagaa ttcgaaccgg atggagagtt tctcgctgat tcagttgaaa
     5161 tcgatgacga gttgaattta gatgatatct taggctcaga tgatatgatt gatgattttt
     5221 cagacttgtt ggatgtttcc cgctatcgta gaactgtgaa cgctgcagat aaaattggcc
     5281 gcagaaaaca tgcaagtcat tttgaaaagt ataagagctt gttcgatcaa gtgcatgcag
     5341 atatcgcatc tggcagacga aaaattatcc cgtttgagca gtatgatatt catgaaaata
     5401 gattctacat ccaaaacggc gtaatgatgt atatcgtttc catttcgaag gataccttta
     5461 tcgctgataa tgggaaaaca aatgccagaa tgcatgttgt ttatgagaat gggacagaga
     5521 ataagaatct gttgttacaa tctttagctt cgtctctata ctcgactgaa cgtcatggaa
     5581 ggatggtgac agagccggta gatgaggaaa cactggctga aagctttgga gaagagttta
     5641 cgacaggtta tatctacgtg ctgaagtcat taagcacgaa tccggatatt agcaagtgga
     5701 ataacctgta taagattggt ttcactcgaa atgatgttga aagtagaatt gccaatgcgg
     5761 aaaacgacgt gacctactta aatgcaccag tgaaggtcat gctttctgct gaagtacaaa
     5821 atgtcaacgc tcagctactt gagcgcactc tccatcatgt attccaggac aaacaagttg
     5881 tctttcagga tggaaacttc aaaaaagcta ctgaatggta tatggttcca ttgaatgata
     5941 ttgaatcgaa aatcaatgaa gtaatcgctg gattacaaaa ataaatatga aaatgcactg
     6001 tactcatgaa tcgaatacag tgcatttttg atatttaatc gcgaacgatt tcaacaatat
     6061 ccgataaatc acaatcaagt gcttcacaga tgcgaagtaa gatatctgtt gttacatttt
     6121 cgcctttatt tagttttgag agtgttgctg aactgatatc agtcattttt ctgagttgtg
     6181 ctttattcag tcctctgtcg attaggagtt tccaaagcgg attgtagcta aacttcatgt
     6241 aatcattcct gttctagtta taaatatcgt atagatatcc atagcgtcca ttgtttttat
     6301 cttcagtaaa gacaatctga tttacttgtt tatcatcata gtttagtgct ggcaagttcg
     6361 ttgtattctc aacgatgata agttgtcctt cgttttgatg atccataaag tatttgaaaa
     6421 gtccttcttt catattgtca tcgccatctt gttttttgcg ttcatctaat ccaagaagag
     6481 gggaatcgat aatgaacaca gaaggtgtgt acttagcgtg attatacaag agatgtcgat
     6541 atgccattac cactactgaa ttgataaacg ctgtatatcc atgtccgttt tcggtccatt
     6601 ttagatttcc attgattttg atgtcgaaaa tatccttggc gaaaaatgcg ttacgaaatt
     6661 cattaggttt ataatgcata ttacgtaaca tggagtccag taactcagac atttctttgt
     6721 agaattctgc atcaaatttc tctttaggtt tataaaggat acgttcgtct tctggctcat
     6781 tctcgatttt ttcaagatca cttttaagct tattttgcat tttttccatc gagattagct
     6841 gttgtttaat atccctgtaa gtttcgattt tattgattcg atcctttaac gtctcagctt
     6901 tcggttttag ttcatcgttg attgctgcat caagcgattt ctttcttgtt ttaagctcat
     6961 caagctgttc cattaactct tgcagttcaa cttcggtatc atgagattct tccgtcaaat
     7021 ctgataattg agagagcaat gaatgtaatt ctgacctagc agtatcgatg taggttgttt
     7081 ggacaggttt acttagttcg ccgttacaga atggacagct atgtgaagta tcatgttcat
     7141 tatgatcatt taataatcgc tcaccatcta cgattagtgt tagtcgttca atatccgatt
     7201 gatattgata atgtaactgt ttgtacctat tcaaaagaag ttgagtttca tgaagatact
     7261 gttccttttg ataaatctct tttatcaaga ttttgctgct ggctaacgca tcattgattt
     7321 gcttttcaat tgtttcaagt tcagcgaaca ttccatcgac aatttgttga aaatcatcag
     7381 agggagcctc aggtaattct gatttagcca cttcgtagtt ttcatataat tcagaaaggc
     7441 ttgaattgat gtagttaatc accgagttcc ttctaatttt tttatgttct tttgtttcgt
     7501 tttcttccga gttcgcaaaa tctgaaccgt atagcagaag taacaacgat gataaaaaag
     7561 ctgtgcgttg ggttgtttcc ccaggaaaaa gaacagaggc gctggtattg actctatctt
     7621 gtggaataag tgtgctaggc gctaatgtcc gccaagttaa tttttgacgt ttgaaatcct
     7681 cattcttgat aatctgataa tcatcatcga tgcctaaaag ttgtaaccaa aaatggctaa
     7741 ttggattttt cccagatcgt gcattatatt tttcataatc tacatattca cctgaactcg
     7801 ttattttgga taccaaaatg gagttgctat caaattctct agttaatgaa tatttgccat
     7861 tagtcgattg aataatcagt tcaactttac tgtatccggt tgcatcggtt gaaaagggag
     7921 cagcgtcttt tttcgcgcca tagagaaaat cgattgccgt aacaatgcta gtttttccgg
     7981 tatttgaagg gccagaaatt atgtttagtc cattggtgaa cttgacagtt gcatcttcac
     8041 gatctttgcc ttttatggta acacgttcaa tataataatt ttgcattatc attgctctcc
     8101 tttaaattcc tttgcgcgtt ggtttatttg tcgcagaagc tcaacgtcac ttttatcttg
     8161 aatgtattta acagattcgt cagctatttg taagtatttt tctgcatata ccgatacgaa
     8221 ttgtgatatc aactttaggc catcttctga taaagcatac ttaaaacctt ctttgctttg
     8281 cgatggttgg ataacatgtt gcagtacaag atttttgact gcatctttaa ctaattcacg
     8341 tcgcgcaata aattcactga atctatagct gttatcacca tttaagttgt aatcggaaat
     8401 ttcaaagtcg cgaccatacg tggtgatgaa gtctgtgatt aaaattctat cagcgctgac
     8461 aggctcttta aattcagaga gcagtaacat tactcgaacc gaaatttcaa atggagtatt
     8521 aaacaaacta ttcatcttca gtcacccatt tgatctttcc gtcattaacc agcatgtggc
     8581 aaatgccttt tttagcagaa ttttgcagca atcccggtat aaacgataag attgatccgt
     8641 ttaatgttat aacagacgac tgctgtagaa cggctttgag tctctcaacg ccatcatcgt
     8701 agtcttcttc atagacttcc tcgatgccgt cgtacatatc ttcttttaaa gcttcaaagt
     8761 gctcagttcc ttcctcttgt gtaaagttat cacggacccc acgacgtatg ctttcagctt
     8821 tatagtaatt tcttcgctgt ctcttgaaat cctttagccg cgaatttggc aggtcgtttg
     8881 tatgctcgta tacttcgtat ccatcggcat ctgcatatgc tgaaaaaaga gctatgacat
     8941 acgtcatctc ctcagctcta atatcaaggg gaactggcaa atcgtcaaag aattttttca
     9001 cttcatcatt ataagagaaa tcaccattag tgttgattct taaacttgcg agctctaatt
     9061 cgctaagctt cggcttggcc ttttttgttt ttacttttgt ttgagaagca attgaggtga
     9121 tttcatcaag taaaagatgg gccactattt cagaaacgtc tgtatctttg gggattacca
     9181 cacctttatc aatcaattgt tgcttaagga cggttggagc atcatctata agacgttcat
     9241 aaataaaagt cgtgcatttg tcgatgttga attttccagc aacccgaact gcatactctt
     9301 gtgttaatgg tcgagtacca ttgtaaaatc ttcgtaggtt atcatcagaa aaaccgaaaa
     9361 tggtggcctc gtcatcattt gaaacactat ccagtaaagc aattaaaaaa tcctgtggct
     9421 tcacgtactc gcttcccata aacggataaa gcgtttgagt aatttcactt atttccaaaa
     9481 tacaacctcc tttgttacgt ccgccgttga ccgccgtttg ccgccgatga ctgacgaaaa
     9541 caagggaggc ctttttctat aattaaaaca tagtagttta actatttaat tttatcattt
     9601 atggttcata aacactacat tatgtttcaa gaaagtgaac gaatttatct taaaagcgaa
     9661 tagtcctact aataaaatat aacaatgcct gagttgcaat agggcaaggt tacaaatact
     9721 gaaaagaact ggttatccag aaaatttttc agatattgta tgccttactt tattgcgctc
     9781 ttttttcgtg atgagggacg gtgtacgtat tggcacggtc cctgtttgtt ttccttgctc
     9841 aaaagctctt tcaggcaaga aagagggatc taatgagtag atttatgaaa acacaccaaa
     9901 acgaccgcag cacttacaag tacgtgtttg agcgtactga tgccaatgga cgtatccaaa
     9961 aagaagtggt tgagattcgc ccaggcgaag acggcgtcac tgagcttaac attcaggagc
    10021 tgcatcgcgc tgacgaccgt gcgatttaca acttttataa aaacgcacgt cctgaacgca
    10081 cagaagaaga aaaagaaaag atcaaggctt ggaaagagaa gttccgtgaa gactttaata
    10141 atgagcacgg ctattatcca atggacgaat acgtggatgg tgaagccaac gattacttca
    10201 aacgtaacta caatttgtcg ctcaatgaat ttgatgatga atgcgatgag ggggacaacc
    10261 ccatccaatc attggtttac gatgcttacc aagataatga agaagatcct cgtatcgatc
    10321 gtttgcgtga agtcgtgacg caattattac cgtaccacca agaaatctat cacatgctcg
    10381 tcactgaaaa catgtctcag actgaagtgg cggacaagct tgacaagaaa aaacagtcaa
    10441 tcaatcgcac ctttggaatc atcaaagaca agatcaaaga attattttag aaaaaagtta
    10501 cgggggagtt tactttcccc gttttccttc gcctgtattg tgtaagggag tagatcctaa
    10561 acaaataaag atgaaggagg accatcatga acctaaaaca caaaatccga gtaaacgtga
    10621 ctgatgacac gggtaacaaa gaaactgtcc tacaaggtgg tgtgcgtcat ttgcctcgct
    10681 ggtttgcacg gtggctgttc ggtaagggca acatggacaa aattctcgtg ctcacaccag
    10741 gggacacaat cgaatcagtc gaaatccgtg aagttaacaa gaagggagcg taatgacgtg
    10801 agtaaaacaa aactatttct tcaggtcgtt gaggacgtcc aagcgttagc tgatagctta
    10861 caagagttgg ctgtggcgat gaaaaccaat gaacctgaac cacaagttga aacaccagaa
    10921 ccgacaatgg aaacaaaatc agaagtcaaa caaccgacgc ttgaagaagt ccgtggtttg
    10981 ttggcacgta aatctcaaga aggtaagtca actgaagtga aagcactgat tgagaaatat
    11041 ggtgccagtc gcttgagtga tgtgccgaca gaaaactacg cggctttggt tgcagatgcg
    11101 gagggattgt agatgccaac acaacacgca gtcctatccg catcttcaag taatcggtgg
    11161 attcactgtc cgccaagtgt tcgcttgtca gagcactttg aaaacaagtc gagcccgtat
    11221 gcccaacaag gcactgatgc tcatacctta tgtgagtaca aactgaataa gttcattggt
    11281 aatgacgtca cagacccaac atctacgctt gaattctacg acgaggagat ggagcagtgt
    11341 gctgaaagtt atgcgacctt cgtcatggaa gaagtcgcta aggcgaaaca gacaacagtt
    11401 gactctattg taatagtaga acagcggctc gacttctcaa ggtacgtccc agacggcttt
    11461 gggaccggcg actgtctcgt cattgcggat ggaaccctat ctgttattga catgaaatac
    11521 ggtctaggaa ttttggtaga tgcgtatcag aacccacaga tgatgtgtta tgccttggga
    11581 gcgttggagc ttttcgatgt tatttatgac attcaagaag tcaagatgac cattttccag
    11641 ccaaggcgtg agaatgtgag tacgtacaca ctgctgaaag aagaattact tcagtgggca
    11701 aaagatgtgt tgtcgcctgc cgcagattta gctttcaaag gtgagggtga gtaccaatgc
    11761 gggaaatggt gtggattctg tccggcgaag aatagctgtc gtgcacgagc agaccataac
    11821 ttgaaactgg cgcaatatga gttcaaacca ccagagcttc ttagtgatga tgaaatcgaa
    11881 gaaatcatcg gtaaagtgga tgacctggtt tcttggagta acgatatcaa ggactgtgct
    11941 ttgaaacttg cactcggtgg caaacaatgg actcatcaca aattagtcga gggtcgctct
    12001 actcgtaagt attctaatga caatgatgtc gctgcggcgg tcatcaaggc tggatacgac
    12061 ccatatgata aaaagttact tggtgtcaca gcaatgacga aggcactcgg taaagagaaa
    12121 ttcaatgaaa tcttgggcga atacatcatc aaaccaaaag gcaagctgac gctagtcgat
    12181 ggttcagata aacgacaagc ggtcacagta aacaacttaa acgaagaatt taaaccatta
    12241 acggaggaac aataatatgt ctaaaacaag caaagtaatc actggtacaa acacacgtct
    12301 ctcatacttc cacggttggg aaccagcatc catcaacggg ggcgcagaga aatattcaac
    12361 agcagtcttg attccaaaat cagataagag aacgattgat accattgaaa aagcaatcga
    12421 tgcagcaatc gaagagggac ttgcgaaatt cggtgggaag aaaccaaaca aagcagaaat
    12481 caagctgcca ttacgtgatg gggatatcga acgcgacgat gaagcatacc aaggccatta
    12541 cttcattaat gcgaactcaa ccactgcacc acaaatcgtg gatcagcacg ttcaaccaat
    12601 cttggatcgt tcacaagtgt atagtggttg ctacgctcgt gtgtcgatta atttctatgc
    12661 ctttaacgca aatggaaatc gcggggtagc ggcagggctt gggaatatcc agtttgtacg
    12721 tgacggcgaa ccactcggct cacaagtatc tgcaagcgat gagtttgaaa ccttggcgga
    12781 tgatgatttc ttggcttaat cattaaaaca aaaacggcca tggcagaagt gctgtcgcct
    12841 gtttgattgg aggaagaaaa tgaaacattt gtcattggat ttggaaacgt atagtagtgt
    12901 cgatttatcg aaatgtggtg tctacaaata ttcagagtcc cccgattttg aaatactgct
    12961 atttgcatac agtgtagatg gtggtcccgt cgaagtggtt gatctgaaga tggacgaaac
    13021 tatcccggct gaaatattaa cttcgctatc agatgaaaca gtgattaaac acgccttcaa
    13081 tgcgagtttt gaacgtgtgt gtttgtcacg ctttctgggg ttggatggct ttttgaatcc
    13141 acgagggtgg caatgcacaa tggtgtgggg tgcgacatta ggtttgccgt tgtcacttgt
    13201 aggagttggt gctgttcttg gtttggataa acaaaagctc acagaaggaa aagacctcat
    13261 ccgatacttt tgcgtgccat gcaatccgac caaagttaat ggtggacgca cgaggaactt
    13321 accacaccat gcgccaacta aatggtcagc atttaagaca tacaatagtc gtgatgtgga
    13381 agttgaaata gctatccaag gaaaactttc caattttcca gtgcttgatt ctgtttggaa
    13441 agaatactgg ctggatcagg aaatcaacga ccgaggaatt gagattgata aggactttgt
    13501 ggaagctgcc gttcagttgg atgaattatc aaaagctgga ttgaatcaac aattgcaaga
    13561 attaactgga ctagaaaatc caaacagcgt gcttcaaatg cggggatggt tactagacca
    13621 cggacttgaa accgagtcac tcggtaaaaa ggcagtggcc gaactactcg aacaacctat
    13681 tgatgaaaat ttaaagcaag tcctggtctt acgtcagcaa ctcgccaagt catccgtgaa
    13741 aaagtatcaa gccatgcaaa actttgcgtg ttctgataat cgttctcgtg gtttattcca
    13801 attctatgga gccaaccgta ccggacgctt tgcaggacgt ggcatccagc ttcaaaactt
    13861 gcctcagaac catatgtcag atttatctga agcgcgtagt ttagtaaaac aaagcaattt
    13921 tgaaatgctt aacattctct acggcaatgt cccgaacgta ctctcagaac tcctccggac
    13981 agcgtttgta ccacgtgaag ggatgaaatt tatcgtatct gacttctcag cgattgaagc
    14041 acgtgtgatt gcttggctgt cgggtgagaa gtggcgtcaa gaagtattca aaaatggtgg
    14101 cgatatttac tgtgcgtctg ccagtcagat gttcggtgtg ccagttgaaa agcatggcgt
    14161 gaatgggcat ctacgtcaaa aagggaaaat tgctgagctt gctctagggt acggaggatc
    14221 ggtgggtgca ttgaaagcaa tggatgctct tgaaatggga ctaactgagg atgaattaca
    14281 gccgcttgtt aattcttggc gagactcaaa cattaacata acggagcttt ggtgggctgt
    14341 tgataaggca gtaaagactt gtgtgaaaga acgacggagc acagcgacgc atggcatcaa
    14401 gtttgaatac aagtcgggtt ttctgttcat ccaactcttg tcaggacgta aattagttta
    14461 tgtaaaacca aagattggtg agaacaaatt cggcggtgaa gctgttacct atgaaggtgt
    14521 cggtgctacc aagaaatggg aacgtcttga aagctatggt cccaagtttg tcgaaaacat
    14581 cgtgcaggca atagctagag acattctctg tttcgcaatg gagaatcttc gtaagtatcg
    14641 cattgtcggg cacgtccacg acgaagtgat tatcgaagtg ccaatggaat cgaaggttga
    14701 agacatctgt cagttgatga gtcaaacgcc taagtgggct gaaggcttgc tactgaacgc
    14761 tgatggatac gagtgtgaat tctatcaaaa ggattagtca atgtggagtc agaaatggct
    14821 ccatattttt ttcaaatttt tttatcaaaa ataaggttcg aattgggttc tttcttcgct
    14881 tatagacgaa ggcaaaaaaa tcaaaaataa attcgagtca gagtttactt tcgaccattt
    14941 tcttcgcctg taagttagaa ggggcaaaat gcctctcctg aaaattctat tacaggaggt
    15001 tcacaagatg aacgaattga aagtttttga aaatgcggag tttggctctg tacgcacggt
    15061 gatgattggc gatgtccctc actttgttgg taaagatgta gcatcaattc ttggctacac
    15121 aaatccacaa aaagcattac gagatcatgt tgatgaggaa gataagacgg tgaacgaatc
    15181 gttcagcgtt aatgggacaa tggctatcct tatcaacgaa agtggattgt actcactcat
    15241 catttctagc aagatgccga acgcgaagaa attcaagcgt tgggtgacaa atgaggtctt
    15301 gccgtctatc cgccaacacg gtgtgtacgc gacagaagat ttcatcacaa agtcaatcga
    15361 agatccagca tgggccattt cagtgttgaa gcagctacaa gagaaaaaag aaatggtggc
    15421 gatgcaacaa caaatgattt tggagatgaa accaaaagtt agctactacg atttgattct
    15481 tcaaaacaat tctgtgatgt cgatttctaa aatctcgaag gactacggta tgtcaccaca
    15541 ggcaatgaac aagttgcttc atgaactggg aattcagtac aagcaaggca agatgtggtt
    15601 gctttatcag aagtatgcca atcaaggcta cacacaatca aaaactcatg cgatagatgc
    15661 agagcgcagc gtgatgcata cctactggac gcagaaaggt cgtttgttct tgtacgactt
    15721 gctgaaatcg aagaagaaca tcttaccagt gattgagcga ggtgataata atgcctagac
    15781 gtaaagatta cagaacatca gtctatgtct gctcgccgtt ctcaggaaat gttgtcgtca
    15841 accataaaaa cactaagcgt tactgccgta ttgctcttga ccgtggctac atgccagttg
    15901 cggcacatct gatgtatcca caattcatgg acgacgcaac ggagcgtgat gtggcaatgc
    15961 acatgtcact tgttctcatg ggcaaatgtg aggaagtctg ggtggtgggt gacaccatct
    16021 cccaaggcat ggcagttgaa attgaacaag cggagtattg gaagaaacat atcaggtact
    16081 tcgatgatga gatgaaggag gtctcaaatg cttaagatga cgttattcac atccaatcag
    16141 gtgggtaatt ccaagaatgg attgttccca accaagcaag tggtgacaga taaagaaaca
    16201 cttgttgcca cggtcaagct ggatcatgtg atggctgagt acaaaggcaa ctatcgcaaa
    16261 ggtgacaact tcatccagtc tgatgtgatt ccacttgatt gcgacaatga ccactcagac
    16321 aatccagatg attgggtagc ccctttcgaa gtggcgatgc agtttcctga tgtgacgttt
    16381 gcggtcagct attcacgtaa caacatgaaa gacaaaggtg gcaaatcagc acgtccacgt
    16441 tttcacgtat atttcccgat tcctgccaca actgataaag atgaatatgt ggaactcaag
    16501 aagcagattc aaagtgagtt cccatacttt gacaacaatg cgatggatgc ggcacgtttg
    16561 cttttcggaa caccaaactc tgaagttgaa atctatgaag gtagtaagtt gatcactgat
    16621 tttctcgaag atgatctgtt tgctgattgg gataatgcgc agtcagagat tagcgaaggc
    16681 agccgtaatt caagcatgag tcacatcgca ggaaagctca tcaaacgcta cggtgcaacg
    16741 gaagatacgt atcacaagta tctcgaacaa tcccaaaaat gtaatccgcc actgccggag
    16801 gaggagctta aagctatctg gaatagcgcg gtcaactttg ggaagaaagt gaaacaacaa
    16861 gaaggctaca ttcctccaga gcagtacaac tctgatttat cacttgagcc aaccgactac
    16921 tcggacttag gtcaagcaac ggtcttagct cgcgagtatg aatgtaagct tcattactca
    16981 ccgtcaactg gttaccttgt ctacaacggc agctattggg aagagtcgaa accaaaagcg
    17041 caaggtgtga tccacgcatt gacagaacgt caactggaag aatctgaaac cgaaattgag
    17101 aagcgtacca aggaaatggt tagtaatggc gcgttcgatg tacttgctag tgttggtccg
    17161 aaaaaggcag tcaccatgtt caacacagcg caacgtcata gttttgattt gtaccaacac
    17221 gcacaagagt ataagaagtt tgctgtcaaa cgccgcgaca gcaagtatct ctcgtcagca
    17281 ttaaccgagg caaagcccat gcttgaaatc gagcaacggt tactagatgt gaacgagttc
    17341 ttgctgaaca caccaacagc aacctttgat ttgcgaacag gcaaatcaca agaccacaac
    17401 tcagaagatt acatcacgaa gcaaacggaa tgtgccccga gtgacgccaa tcagcagatt
    17461 tggttggacg ctctgaacac cattttcgta ggtgaccaag agttgattga ttatgtccag
    17521 atgattgtag gacttgcagc tattggtaaa gtttacgttg aagcgcttat catctcgtat
    17581 ggtgaagggc gtaacgggaa gtccacgttc tggaatgtca tcagccgtgt gctcggtaat
    17641 tactctggca gcatctcagc tgatattctt acaagtcaaa tccgacgcaa tgtgaaacct
    17701 gaacttgcgg aagcgaaggg gaaacgccta ctcatcgcag ccgagcttga agaggggatg
    17761 cgactcaaca catctaacat taagcagctc tgctcgacgg atgaaattgc ggctgagaag
    17821 aaatacaaag acccattcaa gtatgtccca acgcatactt tggtgctcta taccaaccac
    17881 ctcccaaaag ttggagcgat tgataaagga acctggcgtc gtttaattgt gattccattc
    17941 cttgctacga ttgacggcaa taaggacatc aagaattatg cggactacct ttttgaaaat
    18001 gccggcggag ccattttgca atggatcctt gacggtgcga aaaaagtcat cgccgcagac
    18061 tttcatttaa cggttccaca agttgtcgct aatgcaattt ctgaatataa gacggcgaat
    18121 gattggcttg gccatttctt agacgagtgc tgcgagactg ctgttgactt tgaagaaaaa
    18181 tctggtgagt tgtatgcaga gtatcgtgcg ttttgtacgc gaactggtga gtacattcgt
    18241 agttcagccg atttttacac ggctctggag gcgggcggat ttaagcgccg gcgaactaat
    18301 aaaggaaaca agattgttgg tttgcaactc aaatccgagt tcttggaata gttgatttta
    18361 aaggaaagtg taacctgtgt aggtcttata tagaactttt cttaagggat aaaaaattac
    18421 cacctatata aagttatgta ttgcgtattc acgggttaca ccatacttgc agattggaga
    18481 atatctaaat gaaatttaaa cagtggttac aaattggaat cggtaaacgt gatttacgat
    18541 ttgatgagtt ttacgagttt gccatgtcgg atgtcacgta tccttggaag aaatcattcg
    18601 ataagcaagt cgcacatctg atgattcact gcgcagaaaa ggaatacttg gaaatcttgc
    18661 gtgatgcgta tttcgagtat ttgacggtgt ggctatgaga gaaaagcagg ttgagcaagc
    18721 actagtgaaa gccgtcaaaa gggtaggtgg tatttgcccg aagtttacat caccggggct
    18781 tgctggtgtg cctgaccgat tggtacttat gccaaatggg aaacttggat ttgtcgaagt
    18841 gaaggcgcca gggaagaagc cacggtcttt acagcttttt cgtatgaaac aactgacaga
    18901 tttagggttt cagtgtttcg tgcttgatga gattgatcag atacctgagt tactagaaag
    18961 aataggaggt gatgtctgat gaagttcgta ccacataact accagaaata tgcgattgat
    19021 tttatcaaag atcataaaac tgccgccctt ctattggaca tgggcttggg taaaacggtg
    19081 acaacgctga cagctattaa agacttgatg catgatgact ttacaataca acgtgtctta
    19141 attattgcac cgttgcgcgt gacgcaatca acatggccaa ctgaaattca aaagtgggat
    19201 catttgaaag atttatcgta ttccgtggtt cttggaacac caagtcagcg aatggcagca
    19261 ttacaaaata atgccaacat ctatctgatc aatcgtgaga acttggactg gctgattaac
    19321 aaatcgggtt ttacgtttga ttttgatatg gtggtgattg acgaactgtc gtccttcaaa
    19381 aacttaaaag ctaaacggtt cactagcttt atgaaagtca gacacaaggt tgaccgaatt
    19441 gttggattga ccggaacacc ttcaagtaat ggtttgatgg atttgtttgc ggagttcaag
    19501 gttctggata tgggcgagag attgggctat tatatttcac gttatcgaga taaatacttt
    19561 ctacctgaca agcaaaatgg catgcagatt tattcttgga aaccaaggga agatgcagaa
    19621 caagaaatct acgacaagat ttcagatatc acgatttcaa tgaagtcagt cgatttcttg
    19681 gatatgccag agcttgtgat gaatgaagtg ccagttgata tgggactcaa agaaaagcaa
    19741 aagtacgatg aaattaaggc agacttggtg ctacaattga aagaagccac cattgatgca
    19801 gccaatacag cggtgctttc gaataaactg ctacaaatgg cgaatggagc aatctatgat
    19861 gagtttaatg tgagtcatca catccatgac cagaagttag atgctttgga agatttgatt
    19921 gaaggtacaa atggcaaacc gattctgatt gcctattggt tccagcatga tctggagcgt
    19981 atcaaggaac gattcaacgt tcgacagatc aaaacgtctc aggacattat tgattggaac
    20041 gaaggtagca tccccatcgc tgtcattcat ccggctagtg caggacatgg attgaattta
    20101 caagctggtg gttcaacact tgtatggttt ggtcttacgt ggagtttgga actttaccaa
    20161 caaaccaatg caaggctgtg gcgacaaggg caaagtgata ctgtggtcat tcatcacatt
    20221 attgcgaaag atacgattga tgaagatgtg atgttggctt tgaaacgaaa agataaaaca
    20281 caatcatgtt tgattgatgc agtgaaagct agattggagg gaagcaagtg acaccaaaag
    20341 aatacttgaa acaagcgtac cgtttgaata aacgaattga cgaccggatt aaagagaagg
    20401 cggagctcta tcaactggcg gtgacagttt cttccacaac attcgaagaa aagatttcaa
    20461 gtggcacacg gaatactgag gcaccatttg ttcgtaacct tgagaagtgt taccaattgg
    20521 aagctgaaat tgatgcagag attgataagt ttgtagacct caaacgacaa atgcgtggcg
    20581 tgattgatga actcacaaac gtggacgaaa agattgtgtt aatcaatcga tacctgttgg
    20641 aagagagctg ggacaagatt gctgaagatc ttcacgctga cgtgcggaca gtctttcgtt
    20701 ggcatagcaa agctcttgag aacttgaagc taccagatga acccatagtc atttgaaatg
    20761 tgtcagtgaa tgtcagtagc ctttagtgat atcattaaga tgtcaaaaaa gacaaacgat
    20821 actcaagcaa taccagcaat agaagtatcg aacaaatact cgaagccttt gtgggaaaaa
    20881 tccttcaagg gcttttcttt tgcccgaaag gatgtgaccc cacttgccaa tgaaaccagc
    20941 taacccctgt aagttcccag gttgtccgaa ccgtacgcat gatacttatt gtgatgaaca
    21001 taaaacactt gcgaggaaac gttacgagaa atacgagcgt gatccggcaa tcaacaaacg
    21061 ttacggcaga gcgtggaaga aaatacgggc aaggtacgtg gcagcacatc ccctatgtga
    21121 gatgtgtctg aaggaaggac gctttacttc ggttgagatt gtgcatcaca tcaaggaatt
    21181 aagtgatggt ggtacgcatg atgaaaataa tttgatgagt gtctgcaaat catgtcactc
    21241 gaaaattcac atgactcgac gaaacacaaa agataaaaca atcatgtgaa aaataacaag
    21301 ggggggaggg gcggtaatta tccctaaacc cttctaaact gtacagcgcg ctaggccctt
    21361 acgcgaaaaa agttcagttc aaacggggga tagccccaaa caagaaaaag aggtgataaa
    21421 ttttggcaaa agatggaaca caacgcggtg gcagacgacc tcgttcaggg gaacgctcac
    21481 agccactcgt tgataaagtt gctgctggaa agaaagcatc gcggatggaa tttgacttac
    21541 cggatgttga attattaatt ggtgaagata ttggtgaggg tgtagagctt gagggcaatg
    21601 attatcctga accaagtgct tacctttctg caaaacagaa ggatggcact gtgctaggcg
    21661 ctgatgaaat ttataactca acgttccaat ggttgaaaga ccgcggatgc gaaacgttga
    21721 tagccccacg aatggttgag tcctacgctc agaactttgc gcgttttgtt caatgtgaaa
    21781 atgctctgag tcaatatggt ttgctcggaa aacacccgac taccaaggca gtcatggcct
    21841 ccccatttgt ggcgatgtcg cagaacttcc aaaagcaagc aaatgtgacg tggtatgaga
    21901 ttcaacagat tgtaaaagag aatgcatcag tggaatacac aggagatccg aatacggatg
    21961 cgatggaacg attgttacgt tctaagaatg gttgaaagga acagaattta tgatagagaa
    22021 ggttaatccg caacatccag ataaagttgc agatcgtatt gccggagcca ttgtggactt
    22081 ggcatacgca gaagaaaata atccaaagat agcagttgag gttttaattg gtcatggcat
    22141 ctgtcatgtg attattgaaa cttcaactgc tttaaatttg aatgaaatca gccaagcgat
    22201 taatcgtatc gcaggcaaaa ttaaaaaaga tgtggtgatt gtgccccaag ataaagagtt
    22261 ggctgacaat caagcgactg aaattcgttg tggcgataat ggaatctttc gtgggatgcc
    22321 attgactgat gaacagttac agctctcaaa aattgcgcgt gacttatatc aggaccatcc
    22381 atttgatgga aagtacattc ttgatggtga acgcttgata atttgtcaga gccacgcaaa
    22441 gacaaatgag ttaaaacaaa aatatccaaa cgctacagtc aaccctctag gtgattggac
    22501 tggcggcccg gatgtggata caggtgcgac taatcgcaag cttggttccg atatggcaga
    22561 cggagtaaca ggtggcggta tccatgggaa agacttatca aaagctgatg tgtcagtgaa
    22621 catctacgca ttcttgaaag ctcaagaaac tggtatgccc gttgagttaa gctgtgcgat
    22681 tggtgacact gagattgatg gcgtgcctta ttctgaaatc gtggaagttg cgcgtgatta
    22741 tgtgaatgag cttggcgggt ttgagaaact agcagaatgg gggttgttct aatgaacaca
    22801 acagaacgtt ttgagaaagt tgatattgat aagctggtcc cttatgccag aaatgcccgt
    22861 acccacagta aggatcaagt gaaacagatt caagcttcta tgcgtgagtt tgggttcata
    22921 aatccggtga ttgttgataa ggattataac ttgattgcag gtcacggacg tattcttgcg
    22981 gctaaagaag aaggtatcaa gcaggtaccg tgcgtgtttg tggaacacct aaccgaggca
    23041 caaaagcgtg cgtacatact tgctgataac cgactagcaa tgaacgccgg ttgggatgat
    23101 gaaatgttgg cggttgagtt atcagacctg caaggtgaag agtttgattt ggatttgctt
    23161 ggttttgaaa ctgatgaact tgataaatta ctcaacggta atgatgatgc ccaagatgat
    23221 gactttgatg tagatgagga acttgaaaag ccagccttca gtaaaacagg ggacttgtgg
    23281 ttgcttggtc gtcaccgttt agtttgtggt gactcaacaa aacctgaaac ttatgagaaa
    23341 ctcatggacg gcaaactagc aaatcttgtg gtgacagatc caccttatgg ggtctcatat
    23401 caaggtaaag cgggaacgat taaaaatgat aatctgaagg acgaggcatt ttacaacttc
    23461 ttgtatgatg ccttttcttg tatggaaagt gtgatggcca acgacgccag tatttatgtt
    23521 ttccatgcgg atacggaagg cttgaacttc agaaaagcat tcgtggatgc agggttctat
    23581 ttatcaggta cgtgcatctg gaagaaacag agtctagtgc ttggtcgttc gccttaccaa
    23641 tggcagcatg agccggtgct ctatggctgg aagaagaaag gcaaacatca atggtacacc
    23701 ggacgtaagg aatcgaccat ctgggaattt gataaaccaa agaagaatgg tgaccaccca
    23761 acgatgaaac caattccgct gttggcatat ccgattacca attcgtcact cagcaactgc
    23821 atcgtgttgg atagttttgg tggcagcgga tcaactttga ttgcttgcga acagaccgac
    23881 cggatttgcc atacgatcga actggatgaa aagttctgtg atgtgattgt taagcgttat
    23941 atcgagcagg ctggaagcga tgcagacgtt tcagtgattc gagatggaaa gactatcgca
    24001 tttaatgacc tagaaaatcc tgaataaata acgtatcact tgctataaaa tccctttaga
    24061 gtgatatata gactaccaaa agaatacgga agacaaagtt atgtttccaa ctcatgaaca
    24121 aatcgagggt atgaaaaaac gttaccctga aggtagtcgt gtagaactca tcaagatgaa
    24181 cgacccacat gccccagcga agggcacaca aggcacagta accggtgttg acgacattgg
    24241 ttcaatcctt atccaatggg acaacggttc aacactgaac gcggtatacg gcgaagatgt
    24301 ggtgcacatc atcaaaccag acttcgaatt ggtctttcaa aacggtagaa gagaaggatt
    24361 tgaaaccttc aatgatgctt ggcaatacgt gacggacatg atattgaacc acgatttggt
    24421 ctgggttgac cttgagtcaa aaacagaaga taccatctac atcagaaagg ggctttaaga
    24481 tgaaagtgag taaacgtaag atttacaaca tcgcaaagaa ttacatcgtt cagttgcctg
    24541 aacgtggcga cctgaaggcc cacaacagcg accgtgaaga cttcttggac attgcggttt
    24601 ggtgtttgga agatgcctta attgcggcct atgaggcagg cagaaaggac ggtcaaaatg
    24661 aacgcaaaga ttaagcaaca aatcattgag attgcagaaa gtggtctgac aaatatgttt
    24721 gatgcgattg cagtgcagcg aattgcacat gaccggcact tttacgagtt ggtgattttc
    24781 attgaagaac acaaagatga gtacagccgc tttatcttgg agggtgataa atgatggatt
    24841 atcaaattta catgccagat aatgaagatg gcattgaaga aggatactac gattggcaag
    24901 aactggtgca attgctgcgg gataacaaga acaaccctga agccattcag ttcattgccg
    24961 atatgatgga ataaagagat taaggcagct gacaacagct gctttttgcg tagaagggag
    25021 tgagttccaa tcgataatta caaaccaact gaatttatgg acaaagactc ccattatgat
    25081 aagtcacgtg cagattatgc cgtggccttt attgagttgt tgaaacacac gaaaggtcag
    25141 tggttcggga aaccgttcaa actgatgcct tggcaagaaa agattatccg tgacttattc
    25201 ggaacgatta aggcaaatgg ctacagacaa ttcaatacgg cttatgttga aatacctaag
    25261 aagaatggta agagtgagtt agcggctgcc gtggctttgc ttttgacttg cggtgatggg
    25321 gaagaacgcg ctgaggtata tggctgtgct gctgatcgag cgcaggcttc aatcgtattt
    25381 gacgtagctg ttgatatgat tcgccagaac ccagcgttaa gcaaacgagt gaaaatcctg
    25441 acttcacaaa aacgaattat ttatcagcca acgaatagtt tctatcaagt attatcagct
    25501 gaagcatact ctaaacacgg attaaacagt tctgcggttg tatttgatga gcttcacgca
    25561 caacctaaca gaaagctgtt tgatgtcatg acgaaagggg caggggatgc tagaacgcaa
    25621 ccgctattct ttttgattac aacggcaggg aatgatacca attcaatttg ttatgagcag
    25681 catcaaaaag ctctcgacat tattgaaggt cgaaagcatg ataaaacatt ctatccgtta
    25741 atttacggta tcaagcaaga tgatgattgg actgatccag aagtttggaa gaaagccaat
    25801 ccgtcacttg ggataacgat tgcagaagaa aaggtggtgg ctgcttgtga ttcggctaaa
    25861 caaaatccag ctgaagaaaa ctcattcaga cagcttcgcc tgaaccaatg ggtgaaacaa
    25921 gcagtgcgtt ggatgccaat ggataaatgg gacgcttgtt cattcaatgc agatgaagaa
    25981 agtttgagag gtcgagtttg ttacggaggt ctcgaccttt ccagcacgac cgaccttaca
    26041 tccttcgttt tggtgtttcc acctgaagat gaggatgaca agtttgtgat actgccatat
    26101 tactgggtgc cagaagatac cttggactta cgagttaaac gcgaccatgt gaattatgat
    26161 ttgtgggcca agcaggggta catacaaacc actgagggaa acgtagtcca ctatggtttc
    26221 attgaaaaat ttattgagga actgggtgag aaatacaata tccgggagat tgcctttgac
    26281 cgttggggag cagttcaaat ggttcaaaac ttagaaggta tgggatttac tgtagttcct
    26341 ttcggtcaag gtttcaagga tatgagcccg gctactaaag agttgatgaa gttgactctt
    26401 gaacagaaat tagcccatgg cggtcatccg gttcttcgtt ggaatatgga caacattttc
    26461 gtccgaactg acccggcagg taatatcaaa gctgataaag aaaaatccac tgagaagatt
    26521 gatggtgcca tcgcgacaat tatggctctg gatcgtgcaa ttcgttgtgg aaatgataat
    26581 ggtgccagtg tttatgatga taggggattg ttgtttctgt gaaatatgag cttttattac
    26641 aatccattga tataattgtc tttggaggtg tatgtgtatg aatgaatatt tgattcaagt
    26701 agcaccagat aaaatatctt cagacccgga caacacatta atttctagac tgttttatag
    26761 aaaatggtgg ggaaaaattc taactgaaga tttgacaaag tatgctgaag gaattgggaa
    26821 ttatagggga gaaacgatta tttctttcaa cacaattgct ggtgcaacga tacggttatt
    26881 gaaatgctat aatcaaaaga acttttcaat gccagatagt cattatgggc gtttgaaagt
    26941 cattttatat tccgatgata tcaccgaaga gatgaaagtg aaatttgtaa aattctatga
    27001 tttaagtcat actcttgcaa attttatgcc actatatgta tcaaaacctt ctctcaatgg
    27061 tgttaaaggt aaagggccgt atcatgactt tcctgattta ttttattctg atgtaagaga
    27121 taaacgatat ggttcatcaa ctaacgctga taaatttatc gttaataata aagactattt
    27181 ctctaaatt
//
LOCUS       DAYYFD010000036        26662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000036 DAYYFD010000000
VERSION     DAYYFD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.036"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1306)
                     /locus_tag="ACHA25_001403"
     CDS             complement(<1..1306)
                     /locus_tag="ACHA25_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762703.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSITSLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYK"
     gene            complement(1317..1985)
                     /locus_tag="ACHA25_001404"
     CDS             complement(1317..1985)
                     /locus_tag="ACHA25_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8762704.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2115..2699)
                     /locus_tag="ACHA25_001405"
     CDS             complement(2115..2699)
                     /locus_tag="ACHA25_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8762705.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3030..3263)
                     /locus_tag="ACHA25_001406"
     CDS             complement(3030..3263)
                     /locus_tag="ACHA25_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8762706.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3278..4228)
                     /locus_tag="ACHA25_001407"
     CDS             complement(3278..4228)
                     /locus_tag="ACHA25_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8762707.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4225..5067)
                     /locus_tag="ACHA25_001408"
     CDS             complement(4225..5067)
                     /locus_tag="ACHA25_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8762708.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5069..5788)
                     /locus_tag="ACHA25_001409"
     CDS             complement(5069..5788)
                     /locus_tag="ACHA25_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8762709.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6336..6638
                     /locus_tag="ACHA25_001410"
     CDS             6336..6638
                     /locus_tag="ACHA25_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762710.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            6710..7936
                     /locus_tag="ACHA25_001411"
     CDS             6710..7936
                     /locus_tag="ACHA25_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8762711.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8024..8416)
                     /gene="rpsI"
                     /locus_tag="ACHA25_001412"
     CDS             complement(8024..8416)
                     /gene="rpsI"
                     /locus_tag="ACHA25_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8762712.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8432..8860)
                     /gene="rplM"
                     /locus_tag="ACHA25_001413"
     CDS             complement(8432..8860)
                     /gene="rplM"
                     /locus_tag="ACHA25_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8762713.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9394..9861)
                     /locus_tag="ACHA25_001414"
     CDS             complement(9394..9861)
                     /locus_tag="ACHA25_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8762714.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9981..11519)
                     /locus_tag="ACHA25_001415"
     CDS             complement(9981..11519)
                     /locus_tag="ACHA25_001415"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8762715.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11599..12285
                     /locus_tag="ACHA25_001416"
     CDS             11599..12285
                     /locus_tag="ACHA25_001416"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8762716.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12486..13013)
                     /gene="msrA"
                     /locus_tag="ACHA25_001417"
     CDS             complement(12486..13013)
                     /gene="msrA"
                     /locus_tag="ACHA25_001417"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8762717.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13097..13774)
                     /gene="pgmB"
                     /locus_tag="ACHA25_001418"
     CDS             complement(13097..13774)
                     /gene="pgmB"
                     /locus_tag="ACHA25_001418"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8762718.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13771..14901)
                     /locus_tag="ACHA25_001419"
     CDS             complement(13771..14901)
                     /locus_tag="ACHA25_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8762719.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14901..17084)
                     /locus_tag="ACHA25_001420"
     CDS             complement(14901..17084)
                     /locus_tag="ACHA25_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8762720.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17081..18106)
                     /locus_tag="ACHA25_001421"
     CDS             complement(17081..18106)
                     /locus_tag="ACHA25_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8762721.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18151..18978)
                     /locus_tag="ACHA25_001422"
     CDS             complement(18151..18978)
                     /locus_tag="ACHA25_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8762722.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18975..20042)
                     /locus_tag="ACHA25_001423"
     CDS             complement(18975..20042)
                     /locus_tag="ACHA25_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8762723.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20068..20904)
                     /locus_tag="ACHA25_001424"
     CDS             complement(20068..20904)
                     /locus_tag="ACHA25_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762724.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20901..21770)
                     /locus_tag="ACHA25_001425"
     CDS             complement(20901..21770)
                     /locus_tag="ACHA25_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762725.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21840..23123)
                     /locus_tag="ACHA25_001426"
     CDS             complement(21840..23123)
                     /locus_tag="ACHA25_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8762726.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23137..24822)
                     /locus_tag="ACHA25_001427"
     CDS             complement(23137..24822)
                     /locus_tag="ACHA25_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8762727.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25097..26122
                     /locus_tag="ACHA25_001428"
     CDS             25097..26122
                     /locus_tag="ACHA25_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8762728.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7998 a         5380 c         4453 g         8831 t
ORIGIN      
        1 ttttataaag aaattccgga tcatgagtag ctataactac tgcacccttt tcttttaagt
       61 ctttaataag gtcagcaatc ctttccatat taatataatc caacccactt gtaggttcat
      121 caaatagaaa taaattagtt tttgacaatg aagttataga aaccaataat ctttgttttt
      181 ctccaccgga taaatcaaac ggtaattgat ctttcatttt ataaagttcc atttttttta
      241 aaattttttc ttgctgatct ttactaacta tttcattccc cagggctaac tcgctcctca
      301 ctgattctgt gaaaaattgg taatccattt cttgcattac taaaaacggc gatgacaaat
      361 tttttttatt ttcgattatc tttcctttgg tagggcgaat aatcttcatg agtattctta
      421 gtaaggttgt tttccccaca ccgtttttcc ctaccaaacc aataacatct ccaactttga
      481 catcaaaatt tatgtctttt agaatatttt cataagtaat ttcttcaact tttaatacat
      541 ctgcacttac cttttcgata tagggaactt caatcgaact attaaaaatg tcaaatttcc
      601 ttaatgcgtt attgttttca gtttttaaca actgaatagt tgtagttttt acagttctat
      661 ttttaacaaa tagaacttga tcaattaatt ctttcaaata gtaaaaacga tgttcagcta
      721 taacaatagt aaatccttta ttttttaaat cacaaagtaa gtcagcaagt ttcttagtcg
      781 ctttgaagtc caaatttgaa gacggttcat ccaatataat taatttagag tttagcgata
      841 aactacatcc aatagaaatt ttctgccgtt caccacttga tagcgaaaaa atgtttttat
      901 ctaatatatt tgaaagatct aataattcag ctaattcatt gattctttta tccatatgct
      961 cttttgtaaa tccaaagttt tccatcgaaa atactaactc actcgttgta tttgttgtaa
     1021 agaattgtcc tctaggatct tgacttacgt agccaatatt ttttgatata ctttcttgtg
     1081 ttaaggattg tagcgagtta ttttcaaata aaatttcccc aaccaatttt ccgtcataaa
     1141 gtcttggaat aagtccattc attaacttta atagtgtgga tttcccactc ccagaagaac
     1201 cagtaatcaa tgtaagactg ccttttttta ttgaaaaatt aaggtcaaca agcgcttgtt
     1261 ccttaacatc cttatatgaa aaagaaacat tgttaaattc aaacaattct ttcacctcaa
     1321 atcaaaatac ttaagaaaaa aatagtaacg gaaaacagga tgatatagtc taataagctc
     1381 atgtttgttt tatagtaaga acttcttttc acaggagatt ctatcccctt tgttattgca
     1441 ttagcggtca tttcttcagc aatcatagtt gcacgtataa gaacgggaac taacagaaat
     1501 tccaaatatt gcagaggctg aaaaaaagac aagcgaatgt ttctcatttt taaagattct
     1561 ttaatcattc ttatttcaga acgataagtc ggaaagaatc tgatcactac tgtaatactt
     1621 aacattattt ttttcggtaa atgtattttt tctaaagagg ataataattc attgctagta
     1681 tagacactaa ccaacaacca tgagatcatt acgaccggca acattcgaaa tacaatatat
     1741 aaggatgtcg caaaaaatat agtaaaaaac tctatcttga tagcatcaat aattttcatt
     1801 actaaaaaca gaactgaata aatcaagatg aaataaatac atgtctttat tttacgaaac
     1861 atcaatacta aaactgtagc aataatgaaa caccccactt gaaatatatt tccctttcct
     1921 aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca aggttaacgg attgattttt
     1981 gacactcttt cacctccaaa aaataagatc gcactgttat cttaagctga taaaaatgca
     2041 ccgttattca gataacagtg cgatcttatc aaaaagcata actgagaaca attctcaatg
     2101 caagcttttt ttaattaact cagtaattca atccacccag gaacagaaaa ttgatacaat
     2161 cttctggtta atagcatagt cttttctttt gaatagctat gcttcaatat atcatagaat
     2221 gtagcgtaga atgagtaaat gtagaagtgg atttcttctt cattaaagac taaattttga
     2281 ttgatcattc cgatagtctt taacttatct agacaacaga ctgtcttttt cgtcacaaat
     2341 gatgtaattt tttcaacaat atcttgatat ttggttccag ctgattggaa taaaatcaaa
     2401 tcgaatatac gtttattctc aaaaatataa ttaataaatg gatctaattc attgaagttc
     2461 tctctccaac ttttctctat atctatattt tctaagttta agaggtattt ggtaaactct
     2521 tcattataca tattaataag cgcattaata tgcggctgaa taatttcttc aaataacgct
     2581 tccttagaag taaaatgttt ataaaaagca cccgtagtta cgcctgcggt caggcacagt
     2641 tgccttatct tagtttgctg aaatccttct ttttcaaaat ggcttagtcc actttctatt
     2701 aacttttggt gagttataat ataatctctg gtttctatgg gtacttcctc ctaataagat
     2761 gaattaactt tattatctta cacaggactt gaagtttcaa tcgttaacgt ttattactga
     2821 actcattcct tagagctaaa ctaatgagaa agagcagaat aactgataca aaaagcaatc
     2881 ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat ccgagaaaga
     2941 tttaaaaaaa ttatatccag ctattaggat aagtcctatc ataatcactc caatagccat
     3001 tttcttatcc tccctaaaaa taaagtcttt tacttaaaac cttttttgta atcgtcaata
     3061 aattttttcc ctttatcagt taattgaaat cctatatcta aatagtcctc atatctaagt
     3121 ttagccagca tctcacttac agatgctgga ctaacactta gaacttttgc taaatcatta
     3181 ttttttaatt tcttttttct ttttgataat tcctgaattg ctagtaaata aattagtata
     3241 gtagtcgttt taatttgtac catacctacc ctccaaatta tttcgaaagg ccttcagcaa
     3301 ttttatccag gttccatttc atcatcgcat agtaactatc accatcttgt cctttttctg
     3361 caattgaatc agtaaaaatc gttgaataga taggaatatt ggtatctttt gatactgttt
     3421 tcattggtct atcatccaca ctgctttcta cgaataagga gggaactttt gttgtgcgta
     3481 atttttcaac taagtgtttt atttgatctg gtgttccttc ttcttcagtg ttgatttccc
     3541 aaatgtaagc agaaggcaca ttatacgctt tcgagaaata tttaaagcat ccttcacttg
     3601 taacaatcat ttttttatca ttcggaattg aagcaaattt agatttagct tctttgtcta
     3661 gagaatccaa tttttcaata tacttatcta gattttcttt atagaatttt ttattatcag
     3721 gatctttttc cgctaactgt ttttcaatat ttttagcgta aataataccg ttttctaaat
     3781 ttaaccaagc atgaggatct tccttccctt tctcactctg accctctaag taaataacat
     3841 ctatgccatc acttgctgca aaatagtctt tgttttcctc tttgttcgca tttttaacta
     3901 attttgtaaa ccaagcattt cctccagttt ccaagttaac accgttataa aaaatcaaat
     3961 ctgcttttga agttttttga acatcttcag gcaaaggttc atattcgtgg ggatctttcc
     4021 caataggtac gatactgtgt aaatcaattt tatcttttgc tatattttcg gtaatatctg
     4081 ctaaaatcga attagtaact actactttta atttttcgtt cgaagccact ttatctttgt
     4141 ctttactatt tattgaacat gccccgaata taaaaagagc aatcaaaatc cccagacttc
     4201 ccagtactgc tttccatttt ctcatcataa cgatctctcc ttctttctta aaaatttttg
     4261 ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa caatacttga
     4321 tcctgctgct atgttaaaac tataaccaat aaacaatcca agcaatgatg aacttgctcc
     4381 gactgttgca gaaataaata tcatagtttt caagctattt gcatataaat atgctgtagc
     4441 tgctggtgta attaacattg caacaattaa tatagttccc acactttgca tagctgttac
     4501 tgctactaaa gttaatagaa ccatcaatag ataatgataa aaactgacat tcatccctat
     4561 tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa atagaactac
     4621 tataaccaac actaaaaccg aaacgccaat tgtaatccac ttatcaatat cttgtacagc
     4681 taaaatattt ccaaataaaa tatgaaataa atccgttgag ctatttgcaa ctcctataag
     4741 gataactcct agtgctaaaa aagaactgaa agtaatacca attgctgtat ctccttttat
     4801 tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa atacaatcgc
     4861 accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca aaacagcatg
     4921 agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac ctactgttcc
     4981 agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt gaaagtcata
     5041 cagaccatta ataaaatttt gaagcatatt attccccctc tccaataaat atagtatctc
     5101 cataggcttt ttttaaattt tcttcattaa atacagattc tacacttccg tgagcaatta
     5161 gatttcgatt caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat
     5221 gcacaattag cactgttttt ccctgatgtt tcaatttttt taacgtatcc ataattattc
     5281 gttcactaac taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt
     5341 gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt
     5401 gatgattaga atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt
     5461 ctgcattttt aattctttga aaaactttca ttccggcata tgttcccata gatacacatt
     5521 cttttacttt tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct
     5581 tcaccagttt tttattgata tcttcattat taatggagac ctctccctga tgagggataa
     5641 tatttaacat tgctttaagc aatgtggatt ttccagcacc atttggacca ataatacctg
     5701 ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac
     5761 aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca
     5821 cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata gtttagattt
     5881 ttttctgttg ctgaagatta gaatgacaaa actatttgaa actacgcgta tagtaagtag
     5941 ctttcaaaat atataaaaaa ggagcgttct caaatgagaa catgctccta aaactacttt
     6001 attggcgtat ggaaaaatta tacttgaagt ggatagcggg ttgaataagt tgataagcat
     6061 caaaacattt ataaaaatta ttcaagacta tgtctgttcc atatacaaat atgaaaatca
     6121 gtaaactcca tttaactttt ttcgtagtgt gtctttttat acatgtttat cgttattaat
     6181 agtgaatagg aaaaaaacta tttttgatca ttgacaatta aatacaattt ttgaataacg
     6241 atccctgcta ttcgagctag aaactcaagt acgccatgac ctgattagcg acaaatactt
     6301 agagatttct ttttgttctg ggaaaacaaa atgccatgac aatagcagca aataatgata
     6361 cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct tacccaaagc
     6421 aattcaaaaa tttcataact actattttag gagatgattt tatagaatcc agagtctacc
     6481 tgaataaaaa acaaacctgt gcatttctag gaatcgataa taatactcta gataagtgga
     6541 ttcgtatata taatttccca gtaataagag ttggtggtgt agttcgcttt gacgtagaag
     6601 atattaaaga ctttatgaat acatttaaaa taacttgaaa ttttcttctc gccgcatata
     6661 ctaaaattag tgaaatatac tgccttactt tttttaagga gtgaggattt tgcctacaaa
     6721 gctttcaaat gggaaatata aaactaatct gagatatcca aaaaggttta aagaaataac
     6781 aggaattgca tcagaaaagt atcaaaaaac atttccaaat aggcaattgg cgattaaagc
     6841 cgaaaatgat atgaagaaga aaatagaaaa agttttgaga gaggagaatg caaactcatt
     6901 agaattaaaa ggaaaaataa cgtttaaaaa attttacgaa tctaaatggc ttcctagata
     6961 tgaattaggt caaacaatta gatcaaacag gcctcctagt gatataacca ttagtaatac
     7021 aaaagatata tttcggttgc atattttacc tatgtttggt gaatacgcaa tgaattatct
     7081 aaatatcaat actgagataa tatcagatga attaaccaaa aaatcgaaag aatatgcaaa
     7141 tataaaaatc ataaaaggat atgttcgttc catgttcgat attgcagaaa tactaaatta
     7201 tatcgaattt aatcgaacta ctaaaattat tcaaagtatc actgcaccta aaaaaaatgc
     7261 tttagaagaa aaaagaatac aagaaggtaa gcaagcacta tcatcaaaag aacttactaa
     7321 ttggatagaa gcagtaaatg atgattttaa taatcattta cttacttttc atgattacac
     7381 actttttatg attaccctgt atcttggtga tagaaaaagt gagacttacg ctttgcaatg
     7441 gaaatatatt gattttgaaa aacaaacggt ccgtcttaaa catactttag ataaatatca
     7501 aagaaaaaag tttacaaaag gtcgaaaaga taccgtcatt caagtaccag aagttgtaat
     7561 gacattgcta agtgaatgga aatcggtaca agctgatcaa ttgttgaaat taaaaattaa
     7621 gcaaacgtta gatcagtatc tctttactta tactaaacca tctggagaag taaattgtcc
     7681 tgttcatgcc gattacctaa actatcgaat caattcaatt aaaagaaggc atcctgactt
     7741 agttcattta tctccacaca aactccgtca tacttatgca actatagcta gacaaggtgg
     7801 agcagatatg aatcaaattt caaatgcttt aacacactca gatatttcta caacaaagat
     7861 atatgtgaac acaccagata tagttgataa ggctgttttt gaagcttttc agagaggatt
     7921 gaataagtgt gattgatatg tgttcttaaa tgtgttctta aaaacaaaag aagcactttc
     7981 caaataattg aaaagtgcca gaaacgttga tatataaagg atattaacgt tttgagaatt
     8041 gtgaagcttt acgtgctttc ttaagacctg gttttttacg ttcgaccata cgtgcgtcac
     8101 gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg
     8161 cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt
     8221 taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt
     8281 cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc
     8341 cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat
     8401 attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt
     8461 ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt
     8521 gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac
     8581 gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc
     8641 tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat
     8701 tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt ttatttttac
     8761 cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta
     8821 ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat
     8881 tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg
     8941 gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa cgtttttctg
     9001 cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat aaaagggtcg
     9061 tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca caacccctta
     9121 ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat atccataaaa
     9181 cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg caggcttaca
     9241 acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
     9301 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
     9361 aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg gcgctttgcc
     9421 caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt cgataaagat
     9481 atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt ggtaaagatc
     9541 tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag tacgatccca
     9601 gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg tagaataagg
     9661 tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt tcaaatcatc
     9721 catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg
     9781 tgtttggtga acaacaacag acatttggct tagatctgca actaattgat ttaatacttc
     9841 tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac ttaaaataat
     9901 agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt ttcagttcgc
     9961 ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg cagttgagca
    10021 aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg atagattttc
    10081 tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc tatcaattcc
    10141 tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat cacagctcct
    10201 aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac atcagccatc
    10261 atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc tgtgagctga
    10321 ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc ttctagaatc
    10381 gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact gccaaacgca
    10441 tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat cagaccattg
    10501 gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt ttctcctttt
    10561 tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca
    10621 gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac ataacaaaac
    10681 gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt
    10741 cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt agaaagcggc
    10801 ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc tgctgctgtt
    10861 tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt
    10921 cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat atcaaaatat
    10981 ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct ataaaagata
    11041 tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg gctgttttgc
    11101 agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa
    11161 aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc tcggttatct
    11221 gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat caggccatgc
    11281 atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg tttaattact
    11341 tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc tgccatccct
    11401 tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct
    11461 gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga aacgttcatt
    11521 ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt atcttggttt
    11581 tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat cggatcattt
    11641 ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc tagatctcat
    11701 tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct ctcgatcatc
    11761 gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta tttgttggca
    11821 gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc aagaaatttt
    11881 tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt attttacttc
    11941 atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt ttggttgcag
    12001 gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt
    12061 cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc ttggagtggt
    12121 ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc
    12181 gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct
    12241 tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa ggttgtgaca
    12301 aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg tgacttggag
    12361 ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga ccctcagtat
    12421 tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt agctttttcg
    12481 gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct
    12541 cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta atcttctgca
    12601 ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg
    12661 tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc attatagtag
    12721 atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg
    12781 gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg atcatattcg
    12841 atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata agttggattt
    12901 ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg ctcttcaaat
    12961 ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt catataaaat
    13021 cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt gcttgtgaca
    13081 gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa aacgaacgta
    13141 cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga tttatgtttt
    13201 cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca gcttctgaat
    13261 cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct ttgagaaaaa
    13321 cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca tcaaaataac
    13381 ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat cccaatccga
    13441 tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc aaaacggatg
    13501 tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca gcaaaggctg
    13561 atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat cgctctagtg
    13621 actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat ggaatggaca
    13681 gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct acaagaacgc
    13741 catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa aatgaacgac
    13801 ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa
    13861 agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc
    13921 tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag gaacgccctc
    13981 tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc catctagacg
    14041 tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc
    14101 caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca gtcctgcacc
    14161 cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa atctcatata
    14221 aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc ctttttgtgg
    14281 tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat cagtaatgac
    14341 agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg tagcaatttt
    14401 acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa aagcataacc
    14461 tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa cgccgatgat
    14521 gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga
    14581 taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga tttctgccat
    14641 ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt tgccaaatgc
    14701 attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt
    14761 tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa aaacttcttg
    14821 aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt tgaaagaatc
    14881 tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc tttttccagc
    14941 tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt ttttattact
    15001 atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa
    15061 attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt cgttactgta
    15121 atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc agcatgaatt
    15181 ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc ttggaataaa
    15241 tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat cgctttactc
    15301 aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga aacgattggt
    15361 tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg cttgctgact
    15421 tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct gccaggacgt
    15481 tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc ttgcggaatc
    15541 agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg acattctttc
    15601 aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata atgcgccaga
    15661 tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc aatgactctg
    15721 tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc tgctgtttca
    15781 tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc ttggacatac
    15841 tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc tgaagcaact
    15901 tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc ttcctgacca
    15961 gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct tgctttttgc
    16021 cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac ttcaggaaac
    16081 tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg ccctttataa
    16141 ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg gggagtcata
    16201 atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga atcgccttgg
    16261 atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg ctctaaaatt
    16321 tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc
    16381 tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggacgtatt ttttctgatc
    16441 ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc tgctttattg
    16501 aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga tgcactgatc
    16561 aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc aaaattggtt
    16621 tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa cggcttgatg
    16681 accactttca gggctgcctg atggagatta ttttgatcag caacacgaca agtaatgact
    16741 tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc tcccatttct
    16801 aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa atgttcaccg
    16861 tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc tgattctgct
    16921 cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt ccgatagtct
    16981 tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg actcgcatac
    17041 ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta ataacttctc
    17101 ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat ccttgccagc
    17161 catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga gccatactta
    17221 gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt ccatctacat
    17281 cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa aaagccacat
    17341 tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg atcttgccgt
    17401 tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt ggatcccatt
    17461 tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac gcccgaacgc
    17521 tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga atgccataat
    17581 cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag cctggaacac
    17641 cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca tcaagatgat
    17701 tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc aaaatctgcc
    17761 cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata gctggtggtt
    17821 tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg aagtgaaagc
    17881 gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt tgtgtaactg
    17941 atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg
    18001 gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa gcagcatgta
    18061 ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat ttctccctcc
    18121 tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat ttaaatgacg
    18181 cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt tcacttcatt
    18241 ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt gaagtagcgt
    18301 ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat gattatctgc
    18361 taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa tatcatcttc
    18421 ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt gattagagtc
    18481 aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg
    18541 ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac gaagtgattc
    18601 gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg gtaatcgata
    18661 agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct cgcttagtgc
    18721 acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga taaagtcacc
    18781 gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt ctttgattgc
    18841 ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt attcttcaaa
    18901 tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa atgcttgatc
    18961 ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt gatactttct
    19021 tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact
    19081 ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca acatgtgtcc
    19141 tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga tgctcgagca
    19201 aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa
    19261 ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctct taaagaagct
    19321 tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg tttatctgtc
    19381 aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta atcggcaccg
    19441 tatttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat ggtagtagca
    19501 cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc gaagattgct
    19561 acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa ttgagcagga
    19621 tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag tttataatta
    19681 tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac taactctcct
    19741 ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat catatttcct
    19801 aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg caagaatagg
    19861 cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa gtcgacaatt
    19921 tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt cttttcgatc
    19981 tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac taattttttc
    20041 atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga ctaaatcacc
    20101 tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg tcaccaatga
    20161 agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat
    20221 tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat aatcattcca
    20281 cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca atggtataat
    20341 gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt cttcaatatc
    20401 agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa gtgtttgaac
    20461 gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg aaatgatttt
    20521 aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa agctagcatt
    20581 gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc tgatcccaat
    20641 cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga aatacatgat
    20701 gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg
    20761 aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat
    20821 gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat
    20881 tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag caagacgacc
    20941 gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag atcattgaac
    21001 gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt cagtagatag
    21061 acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt tgctagtact
    21121 ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc accgtccatt
    21181 tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac
    21241 gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct agcaactggc
    21301 tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa cagatcattg
    21361 acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga aatagatggc
    21421 gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa cttgcggtta
    21481 agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa
    21541 gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc aagataattg
    21601 cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa aaaactgatc
    21661 tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat gcttggtgct
    21721 aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat tgaaacctcc
    21781 ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag aacttcttgt
    21841 tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt tccgacagtt
    21901 acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat tttcgtgaaa
    21961 ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc aatctcagat
    22021 ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag ttcgactact
    22081 tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc catgtttttc
    22141 ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc gtctagacca
    22201 gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg tgttgggata
    22261 gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat gtaagttgaa
    22321 tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt cacatcatta
    22381 gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc tctcatcttt
    22441 tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt tgacaaagca
    22501 aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc aattccatat
    22561 tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc ccaattttct
    22621 ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc ttgaacccag
    22681 ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag gtttttggct
    22741 ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc cgtctgatca
    22801 atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat tacttctggt
    22861 aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc ttctaccggc
    22921 acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag ttcattgatc
    22981 acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat
    23041 tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc tagcagacag
    23101 gtagtaacaa taattttttt catttttttc cacctcttat tttattattt tcagccacat
    23161 atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa tttgtccaga
    23221 aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat tggaaaaatt
    23281 atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa aagaatctgc
    23341 tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt ttcgaataga
    23401 tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct gcaactcttt
    23461 ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc ctgttttttc
    23521 tacgcctttg taatcatttc gacttcccag cacactttga atataaattg ccggcacgcc
    23581 tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt
    23641 atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg gactggtcgt
    23701 tccatctggg tttttcttgt agtttactcg ggtaccctct ttcttcagct gttccactaa
    23761 ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga taccgtcatg
    23821 ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg tttttaacca
    23881 ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag gtggtagcga
    23941 aaactgatag accatctgtg cttcattctc gccattccca aaataactga tattatcttt
    24001 gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat cagcgattgc
    24061 acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag ttcctagttc
    24121 tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa
    24181 caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca agtctacttg
    24241 atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt
    24301 aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca gtggcaaatc
    24361 aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc aagtactttc
    24421 ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc
    24481 tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa cggagaaacc
    24541 atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg tatctgccaa
    24601 atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt caaaaaattg
    24661 atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc
    24721 tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt tctcagacaa
    24781 atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc cctttctttt
    24841 ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc aatagcattt
    24901 ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta tcaataatta
    24961 atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta aattttcaaa
    25021 atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg ctcccaaaaa
    25081 ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag ctaatgtctc
    25141 aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata aagcaaaagc
    25201 aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg caagaggact
    25261 gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc atacgaccta
    25321 tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg aacaaaataa
    25381 cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa
    25441 aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga tggatgaaac
    25501 acttcttttt gaattggatc aatacaatat tcctattgtg gtaatcggaa aagtcggaaa
    25561 cgactacacg aacatctact cagtggacac ggataatttc caggatagtt acgatttggt
    25621 ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc cgacagaagt
    25681 ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc accacctaga
    25741 tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca tggcagcaat
    25801 gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg atgcattaaa
    25861 atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg atgatctttc
    25921 gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt tgactcggat
    25981 agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc aactaatcca
    26041 ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc tcacagattc
    26101 gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact gaggataagt
    26161 cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg gtattttagc
    26221 gaatttttct tattttccat ctgttttaga tagctgtgga taactataat gtttgtgtta
    26281 aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta taagttttta
    26341 tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg gggatactta
    26401 tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat tcgaggaatt
    26461 attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat caatttattt
    26521 tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg aagatgatcg
    26581 ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca cttttcttct
    26641 agttagacaa attattttag ac
//
LOCUS       DAYYFD010000037        25875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000037 DAYYFD010000000
VERSION     DAYYFD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.037"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..574)
                     /locus_tag="ACHA25_001429"
     CDS             complement(29..574)
                     /locus_tag="ACHA25_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8762729.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ACHA25_001430"
     CDS             complement(586..1278)
                     /locus_tag="ACHA25_001430"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8762730.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACHA25_001431"
     CDS             complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACHA25_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8762731.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ACHA25_001432"
     CDS             complement(1594..2157)
                     /locus_tag="ACHA25_001432"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8762732.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ACHA25_001433"
     CDS             2385..3047
                     /locus_tag="ACHA25_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8762733.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ACHA25_001434"
     CDS             3171..4367
                     /locus_tag="ACHA25_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8762734.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ACHA25_001435"
     CDS             complement(4468..5844)
                     /locus_tag="ACHA25_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8762735.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6748)
                     /locus_tag="ACHA25_001436"
                     /pseudo
     CDS             complement(5881..6748)
                     /locus_tag="ACHA25_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6837..7862)
                     /locus_tag="ACHA25_001437"
     CDS             complement(6837..7862)
                     /locus_tag="ACHA25_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8762736.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7880..8554)
                     /locus_tag="ACHA25_001438"
     CDS             complement(7880..8554)
                     /locus_tag="ACHA25_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8762737.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8738..9631)
                     /locus_tag="ACHA25_001439"
     CDS             complement(8738..9631)
                     /locus_tag="ACHA25_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8762738.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9705..11402)
                     /locus_tag="ACHA25_001440"
     CDS             complement(9705..11402)
                     /locus_tag="ACHA25_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8762739.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11395..12708)
                     /locus_tag="ACHA25_001441"
     CDS             complement(11395..12708)
                     /locus_tag="ACHA25_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8762740.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12708..16649)
                     /locus_tag="ACHA25_001442"
     CDS             complement(12708..16649)
                     /locus_tag="ACHA25_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8762741.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16758..17216)
                     /locus_tag="ACHA25_001443"
     CDS             complement(16758..17216)
                     /locus_tag="ACHA25_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8762742.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17514..18032)
                     /locus_tag="ACHA25_001444"
     CDS             complement(17514..18032)
                     /locus_tag="ACHA25_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8762743.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18034..18651)
                     /locus_tag="ACHA25_001445"
     CDS             complement(18034..18651)
                     /locus_tag="ACHA25_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8762744.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18648..19502)
                     /locus_tag="ACHA25_001446"
     CDS             complement(18648..19502)
                     /locus_tag="ACHA25_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762745.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19499..20410)
                     /locus_tag="ACHA25_001447"
     CDS             complement(19499..20410)
                     /locus_tag="ACHA25_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762746.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20451..21785)
                     /locus_tag="ACHA25_001448"
     CDS             complement(20451..21785)
                     /locus_tag="ACHA25_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8762747.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21993..22823
                     /locus_tag="ACHA25_001449"
     CDS             21993..22823
                     /locus_tag="ACHA25_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8762748.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23067..23316)
                     /locus_tag="ACHA25_001450"
                     /pseudo
     CDS             complement(23067..23316)
                     /locus_tag="ACHA25_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23507..23701)
                     /locus_tag="ACHA25_001451"
     CDS             complement(23507..23701)
                     /locus_tag="ACHA25_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762749.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24206..24391)
                     /locus_tag="ACHA25_001452"
     CDS             complement(24206..24391)
                     /locus_tag="ACHA25_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762750.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25241..25582)
                     /locus_tag="ACHA25_001453"
     CDS             complement(25241..25582)
                     /locus_tag="ACHA25_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8762751.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25582..25812)
                     /locus_tag="ACHA25_001454"
     CDS             complement(25582..25812)
                     /locus_tag="ACHA25_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8762752.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT         7660 a         5546 c         4428 g         8241 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaaaatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggga taccacctaa aatgactaaa aggcaaccaa
     5941 caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa atcccaccta
     6001 aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt
     6061 tgactgttag aagcatgcag atgtttccta agccccataa taatccacag atcatgtttt
     6121 tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg cctaagatca
     6181 ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag ttgacgatga
     6241 tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat
     6301 caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg
     6361 caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc gtttgtgtcc
     6421 attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa ccagtagaca
     6481 atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca ttttgtccaa
     6541 cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg attacaggac
     6601 taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt
     6661 gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc catgccaaca
     6721 tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac atgataagaa
     6781 acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac gattttctaa
     6841 cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt cccttttcct
     6901 tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt tgctaaataa
     6961 agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt aaatccctta
     7021 tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt aactgaaagg
     7081 ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc cggcgtatat
     7141 ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc atcggtcaaa
     7201 acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa ataatttgcc
     7261 tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat gaggtagtcc
     7321 cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc atcgattttt
     7381 gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga
     7441 actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga gtcaatataa
     7501 tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg ttcacggatc
     7561 agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc actcaaataa
     7621 ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct tctcttgctc
     7681 aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc taaataagca
     7741 attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat cgtcaatgca
     7801 ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt aaaaaaattc
     7861 atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt gtttcgatat
     7921 atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg actgtcaatg
     7981 tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt tcccgactta
     8041 agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt ttgctcaaat
     8101 caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta atgccattgt
     8161 ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt aatgcattga
     8221 tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct gcccccatgt
     8281 tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg atcggtactt
     8341 tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga agattaatat
     8401 ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga ttgaaatcgg
     8461 tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat tgtcgaatga
     8521 tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc tttcttcttg
     8581 ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa aatgtcattt
     8641 tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct cttccaagca
     8701 gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt ttctttcgtt
     8761 gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc actagcaata
     8821 tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg
     8881 aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca
     8941 cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg
     9001 tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac
     9061 catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt
     9121 ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg
     9181 gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat
     9241 caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt
     9301 cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga
     9361 cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca
     9421 ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct
     9481 cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa
     9541 ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg
     9601 tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg
     9661 cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc
     9721 attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag
     9781 aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact
     9841 ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata gcttccataa
     9901 gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc
     9961 atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt
    10021 ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct
    10081 gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct
    10141 tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt
    10201 tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca
    10261 aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct
    10321 agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct
    10381 aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca
    10441 aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg
    10501 gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt
    10561 ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata
    10621 ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt
    10681 tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc
    10741 atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat
    10801 gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc
    10861 atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag
    10921 aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat
    10981 accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa
    11041 aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg
    11101 tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg
    11161 tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat
    11221 ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc
    11281 acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc
    11341 cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc
    11401 atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata aaagaattcc
    11461 tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc attttcctga
    11521 ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt cttttacggt
    11581 ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt tcccggattc
    11641 taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt ttgcatccac
    11701 ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct cgtaaccttc
    11761 cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt gtcctgtcgt
    11821 aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca aaggatcatt
    11881 gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat ctaaataata
    11941 tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg gttttccttt
    12001 ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta gataaccgac
    12061 atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg ggtgataaat
    12121 tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca atccaacttc
    12181 tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt ttttcttagg
    12241 tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc cagttgttac
    12301 cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat tgtttctgtt
    12361 cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag cttttttttc
    12421 atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg
    12481 tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat cattttcatg
    12541 gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca acacgaattc
    12601 cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga ctgctgcaag
    12661 cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta ccttgctccc
    12721 tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc catgaagaat
    12781 gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa cggattttca
    12841 ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag ttcatattct
    12901 aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga aatctcaact
    12961 tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc aggttcttct
    13021 cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc aggtgacttc
    13081 acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag ggaagaatcg
    13141 tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact attttgtttc
    13201 tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat tttcaaaagt
    13261 tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc atcgatgatt
    13321 tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc aatgataaac
    13381 ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata cccatttggt
    13441 gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg gtcgtaattt
    13501 aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat cccaggcaaa
    13561 cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag cgggaaggaa
    13621 gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc ttcatggtct
    13681 aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa tgctacccat
    13741 aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc acctgtttgc
    13801 ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt gtcattttca
    13861 tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc tgcatagttg
    13921 gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg atgaataact
    13981 ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa cgtgagtctc
    14041 cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg atatccgatt
    14101 tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat tggcatatta
    14161 ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt tcgcatttcc
    14221 agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg tacaacagaa
    14281 agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg tccgccgact
    14341 ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc atcttcgttg
    14401 gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata acgtggtggt
    14461 ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc
    14521 ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt ttcgattggt
    14581 tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc agccaacggc
    14641 tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc gtaacgaatg
    14701 gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc ctccaggttc
    14761 tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt
    14821 tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg agaaaattta
    14881 taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc tggtggggct
    14941 tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc gttttctcct
    15001 ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg tgagacatta
    15061 aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt agaagagata
    15121 tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg
    15181 ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc atctagtcgt
    15241 tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact tggcgggttc
    15301 tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc agatgacttt
    15361 tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc aacccagtga
    15421 gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt tacttcatct
    15481 tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc attagaagta
    15541 ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc aatcttatct
    15601 tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact tgactgaatc
    15661 ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt ttctggattt
    15721 ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag ccctagtgta
    15781 agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc cgtcaaacga
    15841 ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt attgatagct
    15901 gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg gtcagagagc
    15961 agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt ttgactattg
    16021 gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac gatatcgatt
    16081 ggactgattt cctttttctc gcccccgatg acatctagat agacattgta aagggttgtt
    16141 gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc tacccatttt
    16201 ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc atttccccaa
    16261 agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga tccattatta
    16321 gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact atcatcgatc
    16381 aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt gctttgtgtc
    16441 caatttccta acacttttgt atagtcattc gttgtaaact tcgggtagcg atgctgttcg
    16501 ttttcaactt ctccgctagg atagatatat tcataggggt cttgcgtagc tgttggccca
    16561 agacgctcca aaataccgtt gaataactgg cgttgaatgc gctggagtgc ttgaccgctg
    16621 ggtacgattg gttcgtccag cttttccatc tcattttctt cgctacgttg aatttcagcg
    16681 gaatcttctg tatcagtttc attttcttgt aattcttggt tatctattct ttcatcaacc
    16741 accacttgaa gaggtagtta cccttcgttc acatcgggga tcttttctgt ttcaaaaatc
    16801 aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc ttgaccatcg
    16861 atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc tgttccacga
    16921 acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg tattctacct
    16981 tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc ttctttttca
    17041 atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc ctcctttgtt
    17101 tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg gaaatatggt
    17161 ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc tttcacatcc
    17221 ttatactcct ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac aatgcccatc
    17281 taattataat ttaatttaat tttaatttta aatcaaatga tatccttttt agttaaaaaa
    17341 caccaattat acaacattta ttattaaaat taaattataa aattttataa cgttctcatt
    17401 taacaaaact taagttatta agacaaatga ataaacaact acgctttcct cgaaacaaaa
    17461 aaataaagca caaatgcgca tttacgcact catgctttat tcgtacaatt aattcagata
    17521 acggcagcgc ataggatgcc aataacactt cctaaaatag ctcctcctaa aatgtcagta
    17581 ggataatgta aaagtaaaaa aatcctcgaa taagcgatca aacaagcaaa taaaaaggct
    17641 aatattcctg caactggact taattcgaac aacgcagcta ctgataaaaa ggctagcgct
    17701 gtatgtcctg aaggaaaaga agaagtcttc ggtttacttg tgtaaaggac aagccgacga
    17761 tttttttcat aaggtctttt ccgtcttgtc gtttttttga taaaaagcaa accaacaaaa
    17821 aaatagatca aactgccgat aagaatggct agaccatatt ctctagtatc aggtatcaag
    17881 actaaaacag cagcaaggag caaccaaata aaaccaccat tagagatatt actgataaat
    17941 ttcaaaaaat tgatttttcg actatccgtt ttccgttgct tggcgaactt gttgataata
    18001 ctatcatcga ttttagcaat caaacgcaac atgttattcc tcctttttaa gaaccttttc
    18061 ttgcgtacga tggataaaaa gcaggacttg aaaataaaac aagaaactga aacctaataa
    18121 agcaaaatag attggtcctg caaaccagaa tgcgacaaga aggatcaacg tgcctgccaa
    18181 atagaaaagc gcgcttttga aatcattaaa aaacaagaaa aatgcagtaa tccaagaagc
    18241 ttctttttgt tccagttgaa gcaagatttt gtaactaaag agaagcgcgc ttaatagaag
    18301 caaaaacaac gtagtataca tcaatgtata gccaattccc ccaccccaat agcggaaaat
    18361 ctgataatcg atcaatagaa gaagaaaata aacacttgct aaactatctt gctttccata
    18421 aagagagaag ttctcacgaa actttttcca gaatagtgaa ccgagctttt tctcttcatt
    18481 ttttttgtaa tgataaatca tatacagaac tgttcgactt gcagttccta atgtgaaaac
    18541 aggcaagacg cacaggaacc atagtagctg taaatggata agacgtggaa tccattcaat
    18601 gatttctaag aaataatcat tggctttttt accaatcgat cgattcatca tccttttgaa
    18661 cctcctgtca aatccatccc ttcaatgaaa taacgttgtg caaaaaagaa cagcaaaatc
    18721 gtcgggatca agacaagtgt tgctcctgcc atcagataat tccattgtgt cgttgcttgg
    18781 ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt cttgatcttg gatatataac
    18841 agaggaccta agaaatcatt ccaagctgcg ttgaaggaat tgatcgcaat ggtaatcaag
    18901 gcaggtttcg tcaatggaag catcaaatga ctccaaatat acaaatgatt cgctccgtcg
    18961 atttcagcag cttcgatcaa ttcattattg atcgataagt aaaattgacg catcagaaat
    19021 atattgaacg cattgccgaa gaacgatgga acgatcaggg gcaaataagt acctacccag
    19081 ccgatcttgg agaataatac atattgaggg atcatcgtta caaaccccgg gatcatcatc
    19141 gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa aatcaagctt cgcaaatcca
    19201 taagcaataa aagaatttga aaggacatta ccgatcacta ctagcacagt gatgaataat
    19261 gtatttcttg catagcgcca aaaaggaaag gcggcaatcg tatccagata gttttcccat
    19321 acgatttttt ctgggaaaaa ggtcggagga taacgaacga tttcttccat tggtttcaat
    19381 gaagtaaaca ccatccaaag caatggaatg atcaaaatga ttcctattac tgtcatgagg
    19441 acataactta gtattttttt tgtttgccga ttcacactgg tctttttgaa ataatccatc
    19501 atttgccgtc acctccagaa taatagaccc acttaggtgc aagcttcaaa ttgacaaacg
    19561 tcagaaccaa cacgatcaga aacaggatca ttgacatagc catcgcataa cccatatcaa
    19621 aagctacgaa cgccttgttc cacatatgca gattataaaa aagcaaagaa tttgcaggtc
    19681 cgcctgttcc atttttcgtc atgacataag cttcttgaaa aatttggaaa gaaccgatcg
    19741 tagaggtgat cacatcgtaa aaaataatag gagtgattaa tggcaaagta atacttttga
    19801 actttcccca aacgcctgct ccatcgattt ctgctgattc atataaagat ctaggaacat
    19861 tttgtaatgt agctaagtaa agtagcattg ctcctccaga actccatatt ttcatcaaaa
    19921 tcaacgcagg tttcgtccat tgtggatcaa acagccaagc tggtccaggg atatggaacc
    19981 agcccagaaa tgtgttgatc aatcctgcag atggagaaag cagctgcatc cataagatgt
    20041 agactgctac tcctgataag actgccggta aatagaaagc tgtacggaat acactgattc
    20101 ctttgatttt ttgattgagt actgtggcta aaaacacact gaaaatcgtc gtcagcggta
    20161 cattgaagat gacgaaataa acggtgttgt ataacgattt ccaaaataaa tcatcgtgaa
    20221 cgaacattct tttgaaattt gctagaccaa tgaaattcat cttggatgtc aaattataat
    20281 cggtgaaact accaataact gaaaaaagca taggccctag cataaatacc aagaacccga
    20341 taataaaagg cgagataaat aaataggccc atcttgcttc ttttttcttt cggatagata
    20401 aacgcttcaa attttcttgc ctcctctgta cctagacagt caaaaatgga ttattgattt
    20461 tgcttgacta agttttctac tgctttttgt gcatctgcca atccttcttt tggtgatttt
    20521 gttcctaaaa gaatctggtc aatctgttca ttcactaatg tcgtgtaatc cggagcatct
    20581 aaaggaactg gtgtgatcac cgtattttta aagttttcgt ccgccatttg atagatcatt
    20641 tttcctttat ccgttaggtt gtcattattc actaaattct cattggctgt cttgctcgcc
    20701 atgatatcaa aacttttttc gccgaatttt tcttgtactt ctggagaaga aagatatttg
    20761 atgaattcat aagaggcctc aggatctttt gctccgtatg gtacttccaa gacgaatccg
    20821 ccaccccacg accaatggcc tgaaccttct tctttttctg ggagctgcat cacgccaaaa
    20881 tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat aattgatatt ttgggcacgc
    20941 atggctacta atccggaaat aaatggatca gatacaccag acccaaattc ggcttctaat
    21001 ttattgatcg tatttttccc ataataatct tgccagtctt ggatccactc caaggcttcc
    21061 actttatttt tagtatcaat cttgactttt tcatctttgt caaaccaact agtcccgtta
    21121 tctgcgttta gcgcccatac atcagcccct aaattccata atggataaaa accgatgcgt
    21181 gtaaattctt ttccttcttt tttatctagt ttacgtgctg tttcttctaa ttcatcccaa
    21241 gttgtcggta gatcttgttc actgattcca gcttctttaa ataacttttt attataaaaa
    21301 atgacttgtg tatctgtatt gaatgggatc gcataaggat caccttcata ttctactgta
    21361 tcccaaagct gcggataaaa atccttagaa gtgtcttttt ctacatattt ggagagatta
    21421 gttgcttgct gtgcttctgc tctttgggca acggaattta tgtcctgtac aataacatcg
    21481 ggtgcatttc cagctgttat tgcagataaa gatttagtcc agatgtcacc ccatggctga
    21541 tacttataat ttacatggat ctcgttttgt gattgattga aatcctcaat gatttcttcg
    21601 atagcttctt ttcgtgcacc ggatccccaa aaggaccaga aatcaatatt ttttcttcca
    21661 tcacttgttt tttcagaaga atttgctgca cttgaactgg catcgcttgc attacttgat
    21721 ccgcatcctc ctaataaaaa caatgctgaa cttgccagca accctgtaaa caataatttt
    21781 ttcatatttt acctccaaat tttaatgtaa ccgatgccgg ttttaatata acgatatcat
    21841 tttgttctga aaataacaat ttataaggta tgattctaaa aaaattatta atgataccaa
    21901 tactttatat aattcgcgag cgctttcttt gaatttttta atctttcgta gcagaatgag
    21961 aatgtattaa aaatagatta ggaggaattt tgatgtttag acaccagaaa gaattgcagt
    22021 ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa atatctgcag gagctgatcg
    22081 gcggccaata tggcgagatg agtgtagcta tgcagtacct gttccaaggc tggagctgcc
    22141 gaggagaaga aaaatataaa gatatgctga tggacattgc aacagaagaa atcggccacg
    22201 tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg tgcgcctgtt gaagatcaag
    22261 aagcagcgat gcaaaaagat ccaacaatcg gtgccattat ctctggaatg aatccacagc
    22321 acgcgatcgt agatggctta ggacctcgtc ctgctgacag cgtgggcaat ccatggagcg
    22381 gtgcctatat cattgcgagt ggcaacttgt tagcagactt tagagctaat ttaaatgccg
    22441 aatcacaagg aagacttcaa gtagcacgta tttacgaaat gatcgatgat cgtggcgtaa
    22501 aagatatgct gtctgttctc cttgcaagag atagctatca tcaaaatcta tgggaatgtg
    22561 cagtgaaaga attagaagaa aaagaaggtt caatcttagt cccaagtact ttcccgcgtg
    22621 acaaagaacg aaaagacatt gcttatgact tctataactt ctcagaggga gaccaaagta
    22681 gtgaaggttc ttgggctagt ggcaaagcac tggatggcga aggcgaatat caatggttga
    22741 aagaacctaa gatcgaaggc gctgcaccaa aattagaacc agtaacacca aaaatgtatg
    22801 ggacacctcc attcaagaaa taaatatcta cccgtagaaa cacccgtttt ttgttccaca
    22861 cgctttgtga gcaaaactga ctaaagtcat taatcataac cacccttcaa aagggtggtt
    22921 ttctctagag ctataagctc aaatcaccgg ccagcgccta aagacgctgg ctttcatatt
    22981 ttatgttcaa gccatattgc ctttgccagt tttgcccacc tcttaaaagg tttatgtact
    23041 actccttagc ccttaaaagg atcgtcttac tctcgtacac tcagcttatc taacgcacta
    23101 tcatgttttt cttgttcttg aatgtacttt ttgatggtcg cttcattttg tcctactttt
    23161 gtcacatagt atccttccac ccaaaagtgt cgatacccaa atttatattt tagatttgca
    23221 caatctcttg atgatctcct gaatattggc tcgatattga ttataaatta tttttcttct
    23281 atactttggt gtgaatacaa tgtggtattt acacatctac tttgtacggg ctaaactatt
    23341 tccctttatt gctccacccg catagcaagt ggttttgtat ttcaatcgct cggcgcgctc
    23401 aattaggtct aaagacaata aatctaaaaa cccgctgctt cactgacaga aacaacgggt
    23461 tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa tctattttat ttttttgatt
    23521 ttttataaat ggaaaatgct tcaagaagtc caagaaataa agccgtataa aaacaagaac
    23581 ctatgaaatc tttatttatt agatattcaa cagaaatccc gataacacta gccaacaata
    23641 tatttatcag aagtgtaaaa taaggatgct ttttatagaa tagaactagt gttgttttca
    23701 tagactctcc tttcttcata tttccaatta atcacaaatt cacggcatta ctagaaaaat
    23761 taaaggggcg cagaagtgga aaaatactct tcgactttcc ctacaaacca ggccatcttg
    23821 tgtttaataa ctataccaaa tctaaggtac ctctaaatct aaattccatt tgtggcggtg
    23881 acctccaatt tttaactctt ctatctccca tccagtatac ataaccttga tacttcgacc
    23941 acttatatat cgaacacaca aatacctatc cattgcagga gtactaccta acttagttgc
    24001 tacttgcatc taaataacag ctgaaaagat aggacttact cttagtaaag tatctgaact
    24061 attaattttt aaactatcat ttttatcctc aactacatgc acatctgttg caacttcact
    24121 ttctgtctca tccagataaa tgattaagga atcgggaatt gcaatatctt cagctgacgc
    24181 tggaatagtt gagctaaatt agtcatcata tctttattat aaaataacca acagcaaaaa
    24241 atacaatgaa aaacaaactt ggaactaatg tatcattaat aaatgttgaa aaattcagca
    24301 actcattact gtatgatttt agcagcccaa ccaacatata ccctataaaa gctaataaga
    24361 tggttaggaa tcctttagaa tttttactca ttcatagaca cttccttaaa taacttttat
    24421 tatagaaggt attgtaaata ataatattga caaaaagtga ctcaaaataa aaaaaacctt
    24481 acattatact ggaatgaact gtattgatag tgtacctaaa agctctactg acataagatc
    24541 gtgtaatcgg taaagtaatt ttattaatta tacgggatgt aatcaattag agaataaact
    24601 aatcataaaa ggtactttta cattcataat tgtcgctttg agattctact ttggttcact
    24661 atctgaccca attgcacatt ttcgatcatt acaagtattg gtgtcgttag aacaactaaa
    24721 taaaaactag cggtagattt ttcagaatct atgctaattt ctgtatggat gttccagtga
    24781 tggcaaattt ctcttttcat tgtttttctt gtcgcatgat atgccttttt gtgttattca
    24841 gttaaactat agaaattctt tcctagacag atcttgatcc caaattttac gtttttgagc
    24901 atcgatatag tttaaaatga cattactcat atctaaagag tttagatttt ttaggactaa
    24961 aactgtcagt tgttcttttt cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg
    25021 ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga cttgaggaaa
    25081 tcacctcaat cattcgttta aaatgctata tattctttag tttaagatac ggaaattaat
    25141 gccatcgacc acactagtag aatgaacgac atttacttgg ccttttttta atttcgttat
    25201 gtttagcata taaaaatatt atagagttta cttacaatgc ttaaaacata tactgtataa
    25261 tctgatcaat tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt
    25321 ccttaaaatc aagagatttt aattgtgtaa taattacttg tccttttata tctagctctt
    25381 caggtaatga gtaacgtgtc ggtaaattct ttatagtgga agtaattgga catatgaccg
    25441 aaaacattgt tttacggtta aattcataac gagagacgac tagtcctggt cgtctttttt
    25501 gaatttcttt tcctacggag ggatcaaaat caatccatac aatatcacct tttttaggaa
    25561 tataaattct ttcttctgac actataaaat ttctctccct tcaggagtta aatcttccca
    25621 ttcgtctttc tcataatact cggcctcttc cccaccacta aatggatctt caatttttgg
    25681 aactaacgta attgttccat catcagaata cacaacgata tattcttggt tttctgaagg
    25741 tttctttccg ttatcagaag gtaaagttac cactacagaa cttccttgta atctagattt
    25801 tgtaacaagc attatcatca ctcccttaat aaaagtatac tatataacac taagtaacgc
    25861 aaaattcccc tttgt
//
LOCUS       DAYYFD010000038        24793 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000038 DAYYFD010000000
VERSION     DAYYFD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.038"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..547
                     /locus_tag="ACHA25_001455"
     CDS             65..547
                     /locus_tag="ACHA25_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762753.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            744..1634
                     /locus_tag="ACHA25_001456"
     CDS             744..1634
                     /locus_tag="ACHA25_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8762754.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1960..4434
                     /locus_tag="ACHA25_001457"
     CDS             1960..4434
                     /locus_tag="ACHA25_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8762755.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4537..6273)
                     /locus_tag="ACHA25_001458"
     CDS             complement(4537..6273)
                     /locus_tag="ACHA25_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8762756.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6270..6974)
                     /locus_tag="ACHA25_001459"
     CDS             complement(6270..6974)
                     /locus_tag="ACHA25_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8762757.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7105..7608
                     /locus_tag="ACHA25_001460"
     CDS             7105..7608
                     /locus_tag="ACHA25_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8762758.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7568..7906
                     /locus_tag="ACHA25_001461"
     CDS             7568..7906
                     /locus_tag="ACHA25_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8762759.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7927..9345
                     /gene="ffh"
                     /locus_tag="ACHA25_001462"
     CDS             7927..9345
                     /gene="ffh"
                     /locus_tag="ACHA25_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8762760.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9457..10035
                     /locus_tag="ACHA25_001463"
     CDS             9457..10035
                     /locus_tag="ACHA25_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8762761.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10039..10560
                     /locus_tag="ACHA25_001464"
     CDS             10039..10560
                     /locus_tag="ACHA25_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8762762.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10639..11193)
                     /locus_tag="ACHA25_001465"
     CDS             complement(10639..11193)
                     /locus_tag="ACHA25_001465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8762763.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11446..11721
                     /gene="rpsP"
                     /locus_tag="ACHA25_001466"
     CDS             11446..11721
                     /gene="rpsP"
                     /locus_tag="ACHA25_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8762764.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11733..11984
                     /locus_tag="ACHA25_001467"
     CDS             11733..11984
                     /locus_tag="ACHA25_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8762765.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12108..12899
                     /locus_tag="ACHA25_001468"
     CDS             12108..12899
                     /locus_tag="ACHA25_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762766.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13069..13590
                     /gene="rimM"
                     /locus_tag="ACHA25_001469"
     CDS             13069..13590
                     /gene="rimM"
                     /locus_tag="ACHA25_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8762767.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13580..14341
                     /gene="trmD"
                     /locus_tag="ACHA25_001470"
     CDS             13580..14341
                     /gene="trmD"
                     /locus_tag="ACHA25_001470"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8762768.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14477..14824
                     /gene="rplS"
                     /locus_tag="ACHA25_001471"
     CDS             14477..14824
                     /gene="rplS"
                     /locus_tag="ACHA25_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8762769.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15134..15376
                     /locus_tag="ACHA25_001472"
     CDS             15134..15376
                     /locus_tag="ACHA25_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8762770.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(15464..15610)
                     /locus_tag="ACHA25_001473"
                     /pseudo
     CDS             complement(15464..15610)
                     /locus_tag="ACHA25_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15667..15981
                     /locus_tag="ACHA25_001474"
                     /pseudo
     CDS             15667..15981
                     /locus_tag="ACHA25_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16010..17269
                     /locus_tag="ACHA25_001475"
     CDS             16010..17269
                     /locus_tag="ACHA25_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8762771.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            17322..18218
                     /locus_tag="ACHA25_001476"
     CDS             17322..18218
                     /locus_tag="ACHA25_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762772.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18229..19098
                     /locus_tag="ACHA25_001477"
     CDS             18229..19098
                     /locus_tag="ACHA25_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762773.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIELFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19108..19783
                     /locus_tag="ACHA25_001478"
                     /pseudo
     CDS             19108..19783
                     /locus_tag="ACHA25_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            19989..21653
                     /gene="alsS"
                     /locus_tag="ACHA25_001479"
     CDS             19989..21653
                     /gene="alsS"
                     /locus_tag="ACHA25_001479"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8762774.1"
                     /translation="MAEKMKQGSKSVVERLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            21666..22376
                     /gene="budA"
                     /locus_tag="ACHA25_001480"
     CDS             21666..22376
                     /gene="budA"
                     /locus_tag="ACHA25_001480"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8762775.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            22506..23003
                     /locus_tag="ACHA25_001481"
     CDS             22506..23003
                     /locus_tag="ACHA25_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8762776.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            23188..23448
                     /locus_tag="ACHA25_001482"
     CDS             23188..23448
                     /locus_tag="ACHA25_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762777.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(23810..24049)
                     /locus_tag="ACHA25_001483"
     CDS             complement(23810..24049)
                     /locus_tag="ACHA25_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762778.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            24335..24649
                     /locus_tag="ACHA25_001484"
     CDS             24335..24649
                     /locus_tag="ACHA25_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762779.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8194 a         4213 c         5119 g         7267 t
ORIGIN      
        1 ctaatttttt tagatagaaa attgtatagt atgatgagag aaaaagtagt ttgggggaac
       61 gttaatgaga agaaaagcag taatcgatcg gctttattta tttttttggt tttttcctat
      121 cgttttgcta ccttttacat gggtaaatgc tgttgtacaa ggtgcggaga tattaggcct
      181 gatccaagga agcggattag tggtttctag gaattttcca gctttgactg ttttctattt
      241 agtgataagt gtaggtctct tattaccaaa atctctgatc aatcactaca attggcagct
      301 gctttacttt tcttgcttat tagtatttac atctatgttt tctttgctgc ctcgcttgat
      361 tatcggccct aatggtttga gcgagagtgg attgaaggat tcagcttatt cctccatgta
      421 tttcttgacg atcaaaccat ggttctattt agtaattgcc tcatttattc ttgcaacatt
      481 tttttacttc tggacagaaa tcaggataaa aagtgaacga tctgtatcta taaaaaataa
      541 ctattgatgg tggatcatca gtaaaaaagc atatgagctg cgtaaaatgc atgtttctca
      601 tatgcttttt ttattattat gtaaagaatg cgaaaaacgt ttacatgatt tttacataac
      661 ttaactatat ttttacagca accatacgag ggatttacag tcgactgata aactttagtt
      721 gtaaataaga aaggagcaat aaaatgaaaa aagtattgtt tactctaggt atgatgggaa
      781 ttcttttagc cggatgcggc tcaggatcag gaaatacaga tggctctgcc acacaaaata
      841 attcaagtga ctcaaatgaa caagtaaaaa tcgttgcggt aggttcgaca gcattacagc
      901 cgttagtcga tgcagcacaa gaaagctttg tacaagaaaa tccaaattat caaatctctg
      961 tacaaggagg aggaagcgga actggtttga gccaagtcga agctggagca gttactatcg
     1021 gaaactctga tgtatttgca gaagaaagag acggagttga tgcttcaaaa ttagtggatc
     1081 ataaagtagc agtggtcggg atggcaccaa tcgtcaataa agatacagat gtcaaagata
     1141 tcaccaaaca ggaattgatt gatatcttta caggaaaaat cactaactgg aaggaagttg
     1201 gcgggaaaga tcaaaaaatc aacgtcgtga accgtgcaaa cggcagcggt acccgcgcga
     1261 cattcgaaaa atggggactg gatggtgcta cccctgtgca gtcccaagaa caagattcat
     1321 caggaacagt tcggcagctg gtaagtcaaa caccaggagc aatcagctat ttagctttct
     1381 catatctcga tgattccaca caagcactaa gtatcgacgg tgtagaacca aaagaagaga
     1441 atgtagcaga taacagctgg ggaatctggt catatgaaca tatgtacacg aacggaaaac
     1501 cttcccctga agtccaaaaa ttcttagact acatgatgac agaagaaatc caagaaggac
     1561 ctgtcaaaga attgggctat ttaccaatca caatgatgga agtagaacgt gatcacgaag
     1621 gaaatatcaa ataactagca gataaacatg ccacggtagt cgttactatt aaggaaaaat
     1681 caactatgtt tccccacata tacatttttc ctttaattgt gaaaaatagt tcctcatatt
     1741 ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta ttcgaaaaaa
     1801 agagctacga catagaactg tcgtagcttt ttttttgcta tatgacagga atgaaaggtg
     1861 tttgcaagct taatgtgagg aacactttcc atatctgttc tatactggat aggagaagtt
     1921 tggaaatatc cagaaaacga aatcaatcga ggtagccaca tgaagaataa atggataaat
     1981 cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata aagtcatccg tctttttcca
     2041 tcgtataaag aaggattaac ggcagatcaa gtcaaggaac gaatagaaaa aggtgcagca
     2101 aataattcag tcgatcccac attcaaaaca aatcagcaaa tcgtattgga aaacatattt
     2161 acgtatttca atttgatctt tcttatctta gccattttgc tttgtttagt agaatcctat
     2221 aagaacttga cttttttacc agttatcata gccaatacag gaatcgccat ttttcaggaa
     2281 attcgatcca aaaagatctt ggacgaacta aatgttttaa atacagcaaa agtcaatgtc
     2341 atacgagacg ggatagaaca aacaatagat atagaagacc ttgtaataga tgacattgtt
     2401 attttagaga ctggacatca aattccagca gatgcagagg tcgtggaagg gaaactgcaa
     2461 gttaatgagg ctttattgac aggcgaagca gatgaaataa ccaaagaaat aggcgatcca
     2521 ttaatgtccg gcagtttcat tgtttccgga aaagcttacg cccgattaga caaagttgga
     2581 gcagattcgt atatttccca gctgacagtc aaagccaaga caatgggaga aggagaacag
     2641 tcggagatga ttgcttctct taatcaattg atcaaatgga ttgggatcat cattattcct
     2701 atcggtatcc tgcttttttc tcaaagttac ttttttaatg gaaacacgat caaagaaagt
     2761 gtcgttgcaa tggaagcagc attgatcggt atgatacctg aaggactgta cttgctgacg
     2821 acgattgcat tagcattaag tgcgactaaa ctagcaaagc aaagagttct tttacacaat
     2881 atgaaaagca ttgagacatt ggcaagggta aatgtccttt gtgtagataa aacaggaacg
     2941 atcacagaaa ataaaatgtc tgttcaaaaa gtgatcgtct ctaaaaaaca agaaataatc
     3001 agtcagtcag atcaattgga agcatgcatt gctgactatg caaaagcgat ggcaggagac
     3061 aatgcaacaa tggaagctgt taaagcatat tttacaaaaa ccactgatca atcctatcac
     3121 catgttattc ctttttcttc tgtccaaaaa ttcagcagtg tagcgttggc agacaaggtg
     3181 tatgttttag gagcgcccga aatggttttg cgcgatcaat tgccggaata tgcggaagag
     3241 tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct ttggtgtgta cgatgggatt
     3301 cttgaagaac cagtattgac agaagcagtc acaccattag gctatattct gattgctaat
     3361 cctatcagaa aagaagcaag agccacattt gattacttca aaaaacagca agtagccatc
     3421 aaagtgattt ctggagataa tccgctcacc gtatccaatg tagctgtcca agcagggatt
     3481 tcacatgctg atcagtacgt tgatgcatct aaattatcag aagaagaata tgaagcagct
     3541 gtcgaaaaat atacagtttt tggacgcgtc aagccagaac aaaagaaaat cttcgttcaa
     3601 ttgctaaagc gtcgtaacaa tacagtagct atgacaggtg atggcgtgaa tgatattcta
     3661 gccatgaaag aagcagactg cagtatagcg atggcttccg gaaatgaagc cgccatgcag
     3721 gcctcacaag tggtcttact tgattctgac ttttcacgca tgccagaagt tgttgcagaa
     3781 ggacggcgcg ttgtgaacaa tattgagcga tcagccagct tgtttttagt gaagaatatt
     3841 ttctcctttt tgctctcact gttttcagtg atctttgctc tgacctatcc tttagaacca
     3901 tcacaaatca cattgattag tttgtttact atcggacttc catctttctt gcttgctcta
     3961 gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga atgtgatgga aaaagctgtg
     4021 ccaggtggac taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg
     4081 aatctaaata aaacagatgt ttctacagca tctaccatgc ttttgattgc agtagggttc
     4141 ctcgttctct ataaaatctg ttcaccgctg aataaattca gaagtcagat cattcttttc
     4201 tgtgctagcg gtaacttttt ttcggtcatc tttttgcata agctgttttc aatcaccggt
     4261 atctcagctg tatcactttt gctgttgagc attctatttt tctcggcaga ttcgattttt
     4321 aggcatttga ccacattggt agaatatttg tttgaacgta aaaatgaaaa agagcaaaag
     4381 ccgtttagtc gcgtagttaa aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa
     4441 gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta tctttcgaag caggatgagt
     4501 catccaactt taaaacgcac gagcagttgc tcttttttac attttcggaa tggtaacggt
     4561 gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc gtaccatgaa ggagttgcac
     4621 gtagtctttt acaatcgcta accctaatcc ggtcccgcct gaatgccggc ttcgcgcctt
     4681 gtctacgcgg taaaaacgct caaagatccg ttcctgatcc tcaatgtcga tacctatacc
     4741 aaagtcttgg acagaaaacg atagatcatc tgttgcctga tagcggataa taatctcgct
     4801 gtcgcttttt gaatattgga cagcattttc aatcagattt ttgataattg gataaaggat
     4861 atctgtttta gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca accgtttctt
     4921 ttcgatgatt ggctgataat tttgaacgat ttgctggaaa taaggttctg tataaattgt
     4981 ctggatctcg taagaattct cgccatcttt tgacagctgg atgatttccc gaatcagttt
     5041 atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc
     5101 atcttttgca ccatccagta aagtctcggt aaaaccaatc agtgaagtca caggagtttt
     5161 taattcgtga gagacgttgc caacgaaatc cttttgtagc ttttctaaac gtcgcacttt
     5221 cgttaagtca tacgccaccc ctagaatctg tccggtacct tctgaattag taaagtagcg
     5281 caaactcaag tctaatgtct gccgaccttc tgtaagggtg atctcctgat ggatcagcgg
     5341 atgctcagga gtcacttgat gaatcagctg aatgagtttt ggctcttgaa tgacttctgt
     5401 gtaccgctgt tcaggttgat agcttaccac gtttaaatgt agctgcatct ttggattaag
     5461 cagtagtaaa cgagaatcag catcatcaat caggaatacg ccaatcatca gttcgttcag
     5521 taacgtatat aattgctctt ctgtggtagt atacgcccga tacatcttac tcatttgttc
     5581 gctcaacgca ttgatcgtct gatacaattc ttcccattga tcgggtgttt gcatgattac
     5641 ttccgcctga ttaggatttt gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg
     5701 ctggtttttt tgatagatca gataatagat cattcctgca agaacaagga agaaagacag
     5761 aaagaagaaa aagacccaac ggcgaaaact ctcagttctc ggtaaaaaac cgcttgtcgg
     5821 ttctgcgata cgtaaaatgg cttctaactg gccacttttt ttgactggta atgcgacata
     5881 taaaagatcc ccattcaaag tagtactttt gcgcaaagcg ctacctaggt tcccaccatt
     5941 caatacggct ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt cattgtttgt
     6001 gtcaaataag atctttccag tagcatccat taaagtaatc cgttcgttcg actgatggac
     6061 gaattcttcc aaagcagttt tgttttgcgc tgcttggagg ttttcgacat ctaattgccg
     6121 gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc tgttccacga tctgctgttg
     6181 aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa
     6241 gaagagacgt tcataaattt ttttccattt catcgtttgg gttcctgaaa cttatagcca
     6301 aatcctctaa tagtcaccaa gtactttggg tgttttggat ctcgctcgat tttttctcgc
     6361 aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg
     6421 cggtctagca atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt
     6481 aattcaaatt ctttcggtgt tagttcaatc agatggtcgt ctacggtcac ttgataattg
     6541 gtcaaatcag cagtgatttg tccaatcgat agatattcag gctcagcttc atcttttgat
     6601 tgcttacgag gttcaattcg tctgaatatt gctttcatac gggctaatac ttcgcgagga
     6661 ctaaatggtt tggtcaaata gtcgtcagca cctatttcta ggccaatgat ccgatccact
     6721 tggtcatctt tagccgtcag catcaaaatc ggtgtttcat ttttttcttg acgcagccgc
     6781 tgagtgatct ccatgccatc catcgttgga agcatcacat ctaaaatgat gaaatcaaat
     6841 gattgttcta atgctaattc taatccttcg cctccatctg tggcactggt taccttgtag
     6901 ccttcttttt ccaaattgaa cgtcaagagc gttacaatag aaggttcatc atcaacaact
     6961 agtatttttt tcattcaaat ctcctttaaa taggtttcat tctgtttata ttttagcata
     7021 gaatcagtac aaagggctat tttacaaaag aaataaatgg tataatagga aggcactttg
     7081 ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg catgtttagg cggtctggcc
     7141 tgtcgctatg atggacagaa aaaagaagta ccagaattga tggaactagt tgaaactgga
     7201 gaagctgtca tggtctgtcc agaagtgatc ggcggattac caattcctag agatcccgct
     7261 gaaatcattg gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt atggacgaca
     7321 agcggcgaag atgtaacgga agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg
     7381 caggaaaaac acattgatct agtcatcatg aaggaaaaaa gcccttcatg cggaacccat
     7441 tcgatttatg acgggagttt ttccggaata aaaaaaaatg gtgccggagt ggctactgct
     7501 tattttattc agcaaaaaat gactgtgctc tctgatgaag aatggctgaa aactagaggt
     7561 gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat tttattcgac
     7621 cttattgaca gaaaaacaaa tgaactacat ggaattatat tatgcagatg atttttcatt
     7681 aggtgagatc gcggaagaat acgaagtaag ccgacaagca gtatatgaca atatcaagcg
     7741 gacaagcaag atcttggagg attatgaaaa aaaacttcat cttttttcta attatattgt
     7801 tcgagaacag gtattggaaa atatgactgc atatattaca gataagtacc cggaagataa
     7861 aaaactactt gaatatgtac agcaaattca agcaatcgaa gaataaataa aaggaatgaa
     7921 gaaaatatgg catttgaaag cttaactgaa cgtctccagc aggcgatggg aaaaataaga
     7981 aaaaaaggaa aagtatcaga agctgacgta aaagaaatga tgcgggaaat ccgccttgcc
     8041 ttactagaag ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa
     8101 cgtgcggtag gggcagaagt gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc
     8161 gttgatgaag aattgacgat cacacttggt tctgaaacag cagagttaaa taaatcacca
     8221 aaaataccaa ctgttatcat gatggctggt cttcaagggg caggtaaaac gacctttact
     8281 ggtaaattag ccaattattt aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg
     8341 gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt
     8401 cctgtatttg atatgggaac tgaagtcagc ccggtagaga tcgtccgtca agggatggag
     8461 ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata cggcaggacg tctgcatatc
     8521 gatgaaacat tgatggatga gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt
     8581 ttgcttgttg tcgatgccat gactggtcaa gatgccgtca acgtagctga tagcttcaac
     8641 cagcaattag gtattacagg ggtcgtcatc actaaacttg atggggatac tcgaggcggg
     8701 gcagcactat cgatccgctc agtgacaggc gcaccaatca aattcatcgg ttctggtgaa
     8761 aaattgactg acttagaagt attccaccca gatcgtatgg ctagccgtat ccttggtatg
     8821 ggggatatgc tgacattgat tgaaaaagcg cagcaagact atgacgagaa aaaagcggaa
     8881 gaacttgcac agaaaatgcg ggaaaattca ttcgatttca atgactttat tgaacagctt
     8941 gatcaagtga tgggtatggg cccactggaa gatctaatca aaatgatccc aggaatgaat
     9001 caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg
     9061 atggtttatt ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa cccaagccgt
     9121 cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa
     9181 caattcaagg aatcaagaaa aatgatgcag caaatgtcca aaggggatat gaacatccca
     9241 ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat
     9301 cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga aataagatga aaaaaacagc
     9361 ttcggtacag actgaaaatg tctactgccg gagcttttct atcagcaatt tttattcttt
     9421 actaatacaa ttactctaaa cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa
     9481 acagaaagta tgcggcaata tcatgatact gtctggggtg ttcctgaata tgatgaccaa
     9541 aagctgttcc gaaaattgat gcttgatatt aatcaagccg gattgagctg gcagacgatt
     9601 ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat tcgatattaa aaaagtcgca
     9661 aattatggag aaacaaaaat cagtgaacta atggaaaaca aaggaattat tcgtaaccga
     9721 agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc
     9781 ggcagttttt cagagtatct ttggtcgttt tctaatggaa aaatcgttga ttcttcttat
     9841 cattcacaag aagaagtccc aacaacaagt cctctttcag atgaattagc aaaagatcta
     9901 aagaaaagag gatttaaatt tatcggaagc acaacaatct atgctttttt agaagctgtc
     9961 gggatcatca atgatcacct tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc
    10021 agaaagggcg aataaagcat gcgaaaaatc atattttatg gtgctataag tttagatggc
    10081 tatttggcaa cgaaaacgga tgatcttcaa tggctttttg acactccaac aggtaagcaa
    10141 accacttatg aagcttttta tgaaacaatc gatacgacca tcatgggcag aaaaacttat
    10201 caggaagcaa aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt
    10261 ttttccaccc acgcacaatt acaattgcca gatgctgagg tcgtaaacga agatcctgtt
    10321 gcttttctaa aaaatctaca aatgacagaa ggaaaagata tctgggttgt gggtggcggt
    10381 cgattgatca aaccgctatt ggaacatcaa atgattgatg aatggtatat ccagattacc
    10441 cctgtattat taggggacgg gattccttta ttccaaaaag gaacatatga agcgcgtttt
    10501 gaaatgctag acacgacacg attcggtgaa tttatcgaat tgcattatgt acgaaagtag
    10561 aagtaaataa aataaaaaag gcaactatat gcagaaatgt cctgtacata gttgcctttt
    10621 ttattaccgt tatgcttttt actctgttaa tttgttagaa aaatctagat acgcatctaa
    10681 aatcgtttgg aagaaactta atgttccttc aacgatatta ccattttcat caattagatt
    10741 cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt ggcacattca aaaagacaag
    10801 tgattgtcgt agatggtggt ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac
    10861 gactaaacct ggtttatgat cccaaacact gtgtccataa ggtcttgagc ctacatctaa
    10921 agcatttttc aatactgctg gcacagaacg gttgtattca ggagtaacga aaacgacacc
    10981 gtctagtccc ctcacttcct cacgaaaacg agtccattca gcaggagcct gttctggagt
    11041 ttcgagatcc tcattgtaaa atggcagatc atcgattttt acaaatacac tttcgtaccc
    11101 ttcaggaagc aatttcgcaa aagcttctgc tacttttttg ttgtaagaat cttttcttaa
    11161 actaccaata aaaaatccaa ttttttttgt catacgattt ttcctccttc aaactatcta
    11221 taacaatctt acaaaaaata agtaactgga gcaaataaaa acacttactg acaaaagcta
    11281 catagaagat agattactta tttttaacaa aaaagaagtc taaaatcttc tatttcctaa
    11341 aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg
    11401 ttacactaca tcttgtgaat aaaacaatgg aggtggaaat aaataatggc agttaaaatc
    11461 cgtttaaaac gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt agctgattca
    11521 cgttctcctc gtgatggacg tttcatcgaa actgtaggta cttacaatcc tttgaaagac
    11581 cctgcagaag tagttttaaa agaagactta gttcttgact ggttatcaaa aggtgcacaa
    11641 ccttctgaca ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa acaccacgaa
    11701 gctaaattct caaagaaata aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa
    11761 caatcgttcg tccgctagtc acttatcctg atcaagtcaa gttagacgtc gtagagtcga
    11821 aagactttta cgagtacaat ctaacagtgg ctcctgaaga tattggccgt atcattggaa
    11881 aacaagggcg tgttgctaaa gcaatccgca caatcgttta cggtgttcgg atcaatgcac
    11941 cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac gtgctgtact
    12001 cgaattttcg agtgcagctt ttttgttttt ttattaattg aagaaatgat tatttggcaa
    12061 aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg agaaagaaaa
    12121 tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact caagaatcag
    12181 acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa aaaagtagta
    12241 ccaaaacggc taccaccact gaacaaacaa tggctacctc aaataatgag aaaaagactg
    12301 ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat gtaccagtct
    12361 cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct attttatatt
    12421 acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa acgcctattg
    12481 caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa gttttgcagc
    12541 cctttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc acagggtatc
    12601 agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg cgtattcgaa
    12661 tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa atcgtagctt
    12721 atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact gttgatatgg
    12781 gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat gcctatacga
    12841 tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag aggttgtgaa
    12901 atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta atcaaaaata
    12961 gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta gaattgcctc
    13021 ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt gactgaatac
    13081 ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg tgttatctca
    13141 acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgttaacact attccaagac
    13201 ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa ttttgactta
    13261 ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga tggcatatta
    13321 aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta ccatgagatc
    13381 atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga aatcttgtca
    13441 ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca
    13501 tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg tgtagaaatt
    13561 ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa gaatgtttga
    13621 aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat tagatatcaa
    13681 tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg attatccata
    13741 cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca tccaagccat
    13801 tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag ctggtaaacg
    13861 attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat tcatttgtgg
    13921 tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag tttccttagg
    13981 agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg caacagttcg
    14041 gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt attcaaccgg
    14101 tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga aagtaccaga
    14161 agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag aatcactaag
    14221 aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac ctcaaatgac
    14281 gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg aagccaagtg
    14341 actttttcta aaatattctt tacagtaggt tgataatatg ttagtatttt atggtgagtg
    14401 caaaagcact taactattac gatattccgc tatgagaaaa gctcataaga atatttggaa
    14461 taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac aattacgttc
    14521 tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg
    14581 tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg gtgctggaat
    14641 cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac gtacattccc
    14701 attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc
    14761 aaaattgtac tacctacgtg cattacacgg aaaagctgct cgtatcaaag aaatccgtcg
    14821 ttaatcattg ttatcagact gagaaagaac ccttggtatt caagggttct tttttcttag
    14881 catgtaattt gaacaccctg atccagtttt tttctttaat tattggtagg ggagaataat
    14941 ggattagaat aaatctacct ataagtagta gtaaaaaaaa gagcactatg ataatatctt
    15001 tcttaagagt aaccgcttta ataaaaaatc cagaatattc taacataaat ggagtattct
    15061 tagaatcaaa aggagaaaga agatggttta tacttatacg gagcaagaaa aaatgaaagt
    15121 ttgagacaca ttcatgaatg atggaaaagt aaaacaagta ccaagttcaa caaaaaagaa
    15181 aaacatccta ttgaaagaag tcttgaaacg ctttgaccat ggagtgactt atacagaaac
    15241 agaggttaat tcgatactgc tgaacgtatt ttctagtggc gattatgtcg aacaaagacg
    15301 ttatctaatc acatttggct ttttcaaaag aagttcagac ggtcgggcat atcagatgat
    15361 gggaattgaa aattagaagt tttaatttat actcattgaa aaaagttcaa gtatgcgata
    15421 ttggttctac accgcatact tgaacttttt ttgtcatctg tatctataat acaaagccaa
    15481 gaacgttatt caaaatatgg atagctatga taaaagctaa tgagcgattc gcagttaaat
    15541 aagcaatcgt cagtaaaatc gttccaggag cgtacatgaa aaaatcaatt ggatgcgtta
    15601 catgaataaa gataattctc ttttatttgt acgaataaat accaaaacgt acctcctaaa
    15661 attagattca aagaagatag caaagaggaa aaagatagtt ctgcagcagc tatagcggca
    15721 tgtggattgc tagaactttc caaacaactg cctgtttcag atcctcgtca tttcgattac
    15781 gaagagatag caatcaaaat cttggaagaa ttacagaaaa actatacaac gaaacgagtt
    15841 aacggatcaa acggtctgct tctccatgcc gtttatgata aaaacagctt aaaaggtgta
    15901 gatgaatgtg tgatctgggg cgattacttt tatgtagaag gaatcactcg tctagctaaa
    15961 acatggtact gttactggtg atgtataaga gaagaggagg attcatataa tgaagcgatg
    16021 gaaaaaaatg atgattggat caatagtttt aggaatgtca ctcgtatttg gtgcttgtgg
    16081 taatcaagaa gacagtagtg aagaatcagc aaatgaaatt accgtttcga catggaatta
    16141 tgaaacaaca cctgaattca aagcgctctt tgaagcattc gaaaagaaaa cgggaatcaa
    16201 agtgaaagca gtagatattg cttcagatga ttatgatacg aaactcacca cgatgctttc
    16261 ttctggagac acgacagatg tattgacgat gaaaaatctt ttgtcgtatt caaattatgc
    16321 tttacgagat cagttagtcg atcaaacaga acgaattaga gaattagaca ctgatgctgc
    16381 tgaagaaaca tatgaaatgt atgatatcga tggaaaaaca tatgcattgc catatcgaac
    16441 ggacttttgg gtactatatt acaataagaa aatgtttgat gatgcaggaa tcgaatatcc
    16501 aaaaaatcta acttgggacg aatatgaaga tttagcaaaa aaacttagta aaaatgacgg
    16561 acaagtttac ggagcatatc aacatatctg gcgttcgaca atccaagcta ttgcagctgc
    16621 tcaaaacgag gcgaatcttg ttgaaccaga ttatcaatat atgactgatt attatgatcg
    16681 agcattgagg atgcaaaaag aaggcgcgca gatggatttt ggcacggcta aatcaacgaa
    16741 agtaacctat cagtctcaat ttgaagaaca aaaagcagca atgatgtaca tgggaacttg
    16801 gtatatggct ggcatactcg caaacaaaga agcaagtaag acagaagtag aatggggaat
    16861 tacagctatt cctcagaaga aaaaaggaga atcggtcacg acgtttggct caccaacagc
    16921 atttgctgtc aataaaaact caaagaaaca aaaagcggcg caagaattta ttgaatttgc
    16981 agccagtgaa gaaggagcaa aagtattagc aggagtagga gtagttcctt catatcgtac
    17041 ggatgagatt gatcagcttt attttaatct cgctggaatg ccaacggatg aaatttctaa
    17101 aaaagctttt tctcctgatg aaatcaaact tgaatttccg attgatacgt atgggcctgc
    17161 aatcgataag attttacaag aagaatatga cttagttttg gttggagatg aaacgccgga
    17221 aaaaggaacg gtaaatatgg agaaacgtgt agctgcggaa aaagaataat catgaaagga
    17281 gagaagccaa gagataaaac actcttggtt tcgtcatcga aatggagaat agtataaata
    17341 agaaagctac aaatcatttg cgtagaaaac gaacgtggat cgcttattcg tttatcgccc
    17401 cgaatttcat tggttttttc gtttttacat taataccaat cctgttttcg cttgtactag
    17461 catttatgaa atgggattct ttttcaacgc cagaatttgc tggactagat aactttcgta
    17521 aaatggccaa cgatgagaca ttttggattt ctttgaaaaa tactttttta tataccgctg
    17581 ctgtagtgcc tggcacactt gtttgttcgt tagggctagc tttactacta aatcagaaaa
    17641 ttagagggat gaagcttttt cgtacgatat ttttctttcc gtatgttact tccttagtag
    17701 caattgctgt tgtttggaat atgttgtttc atccaacagt gggacctatt aatatctttt
    17761 taagacgatt tattgaacat cctccaggtt ggacttcaag ttcaaattgg gcgctttggg
    17821 caattgtaat tgtcagtatt tggcgaggga tgggttatta catgattcta taccttgcag
    17881 gactacaaag tatccaaaaa gaactatatg aagcgtcttc gctagatgga gcaaaccgtt
    17941 ggaaacaatt tttgaatgtg acattacctt cgttgagacc aacaaccttc tttgtaacga
    18001 tcatgcttgt catcaattgt ttcaaggtgt ttgaccttgt gcaagtgatg acggatggcg
    18061 gccctggtcg agcgacaaat gtgctggttt accaaatcta caatgaagca ttcaaaaaat
    18121 ttgattttgg ttatgcttca gctatttcac ttgttttgtt cgtaattgtt ttgggcgtta
    18181 cggttgttca attttcttgg aataagcgtc gtacttagga ggtgtgatat ggaagagagt
    18241 gttcgaaatg aaagaaaaaa agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg
    18301 ttgggtatga ttctgctgat tgtattagct ttattgacga tcattccttt tttatggatg
    18361 gtttctgctt cattcaaagc aaataatgaa gtgttaacga ttccgattca atggatccct
    18421 aaaacttggc atttagagaa ttatctggtt atttggtctc gtattccttt agtgacattt
    18481 tttaagaata cagctttttt aagcgttgtg atcacgttga ttcagttgtt tacttctagt
    18541 tttgctgttt acgggttttc tgaactaaat tttaaagggc gcgatatttt atttttagca
    18601 tatatcggca cgattgctgt cccttggcag tcttatatga tcccgcagtt cattatgatg
    18661 agaaaagtgg gtttaacaga tacgttgtgg tcgttggtgc tattacaagc gttcaatgct
    18721 tttggtgtgt tcttgttgaa gcaatattac agtagtatcc cagattcttt atgtgagagt
    18781 gcgcggatcg atggcttatc agagtgggaa atctatcgca agatcatttt acctcttaca
    18841 aaaccggcga ttgccagttt gacgatcatc acattcgtta atacatggaa cgattatatg
    18901 gggccattca tttatttgtc ttctacggaa aataaaacaa tccagttagg gttgaaaatg
    18961 tttattgagc tatttgatgc agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt
    19021 cttcctgttg cttttatatt tttagcgatg caaaaatatt ttgtagaagg gatcgcttca
    19081 tcaggaatga aggaatagga ggtgattatg aaagagcagc gttttgacaa aaatgtttat
    19141 atggaattct tttattggtt gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc
    19201 aatatcttgt atatagcagc tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc
    19261 tttatttttt ctttactttt tattggtcca ggttgcatcg tgatgatagg tatgacagaa
    19321 gaattagcaa aagaaaaaag tttttcacca gtacgtttgt tcataaagac gtttcgccaa
    19381 ttttggctaa agggattttt ttattggcta tttgcttgga tagttagtgt gataatgatt
    19441 tttgattgtt tcttttttat acgtttttct tatggaaaat ggttgattcc gttgtttgtt
    19501 ttacttgctt gttttacttg cttgtttatc agtttccttt agcatcaact gctggtattt
    19561 tcaggtgaga aatccagcgt cgaaaccaaa tcaagttcta cggatagcgt tctactatac
    19621 gttgaaaaaa tggtatgtga gtttactaga ttttttacta ttgacaagtt tgtttctctt
    19681 ctttttcgta aagccccaat ggtgtattct actaggtcct tctatcgtat ttggtttgat
    19741 ttacttcaac aatcgtaaat tgatgagaac tatggatctg tagactttat aaattgaaac
    19801 tttcaattaa gaatgacagt tttttatgta aaaactgtca ttctttttca ttttttgaaa
    19861 catatggcta aacaaatgaa ataaaacatg ctatactttt gaatgaaaca ggtttagaaa
    19921 ctgtatcact tatttgtgat tatttctaac taataattaa aaataaagca taaaaaggac
    19981 gatgttcaat ggctgaaaaa atgaaacaag gttcgaaatc tgtagtagaa aggttgatta
    20041 atcatcatgt tgattatgta ttcggtatac caggtgcaaa aatcgatggt gtatttaatg
    20101 aattagagga tcaaggtcct gaattaatcg tgactcgtca cgaacaaaat gctgcattta
    20161 tggcacaagc aatcggacgt attacaggag agccaggagt agttatcgca acaagtggtc
    20221 ctggtgctag taacttagct acaggtttgg taacagcgac agcagaaggt gacccagttt
    20281 tagcaattgc cggacaagta aaacgaagcg atctattgaa gttgacccat caaagcatgg
    20341 ataatgcagc actgttccag ccaatcacaa aatacagtgc agaaatccaa gatccagaaa
    20401 caatttcaga agtgattgcc aatgcttatc gtatggctaa gagttcaaaa aaaggcgcga
    20461 gctttatcag tatcccgcaa gatgtagtgg atgcacctgt ccaaggtaat gtcatcaagc
    20521 cattgagcga tcctaaatta ggttctgctt ctgcagatga tatccgttac ctagctgaac
    20581 gcattagaga agcaaaacta ccggtactat tagtaggtat gcgaggatca agtgaaaaag
    20641 aaacgttagc catcaggcaa ttagttgaaa agacagcatt gcctgttgta gagacattcc
    20701 aagcagctgg agtgatctca cgtgaattag aagcgcattt ctttggtcgt gtgggtttat
    20761 ttagaaacca accaggagat atgcttttga aacgaagtga tctagttatt gcaattggct
    20821 atgatccaat cgaatatgaa gcaagaaact ggaatgcgga aaaagatgcg cggatcattg
    20881 tgatcgatga agcgccagct gaaatcgatc cttttatgca gcctgaaaga gaattgatcg
    20941 gtgatatctc agccactctt gatttattga caggttcatt agagcctcaa caagtgtcag
    21001 aagatgcaaa agaatattta gcatctcttc aagcaaaatt aacggagcgg gacattgttc
    21061 aatcaaaagg agaagcaggt atcttgcatc ctttagaagt tattaacacg cttcagtcaa
    21121 aagtaacaga tgatatgact gttaccgtag atgtaggtag tcattatatt tggatggcac
    21181 gccactttag aagttatgaa cctcgtcatt tgttattcag taatggtatg caaacgcttg
    21241 gtgttgcatt gccatgggcg atttctgctg cattggtgcg tccaaataca cagatcgtat
    21301 cagtttccgg cgatggggga tttttattct ctgcccagga tttagaaacg gcggtacgta
    21361 aaaaattgaa tatcgttcat ttgatttgga atgatggaca ctacaatatg gtagagttcc
    21421 aagaaaaaat gaaatatcag cgggcgtcag gtgtagactt tggtccagtt gattttgtga
    21481 aatatgcaga agcttttggc gctaaaggaa tccgagctac aagtgtagag gaattagaaa
    21541 aagctttaga agaaggattt gcgacagaag gacccgtaat tattgatatt ccgattgatt
    21601 accgtgataa tgagaaattg ggagaaacaa tcttaccaga tcaattctat taaggaagtg
    21661 tcgacatgac tgaaaaaatc ttataccagc acgggacatt aggagctttg atggccggct
    21721 tgatggatgg aacagaaacg attgctcata tattagaaaa aggaactctg ggattgggaa
    21781 cattgcatgg gcttgatggt gaagtcatct ttttagatgg cgtagcttat caaggaagat
    21841 cagatggctc agttgcccaa ttagatggaa gtgaacttac cccctatgca gcaattacag
    21901 attttacgcc agatactagt ttttctgtct caaaaactgc agatggagaa caattgaaaa
    21961 aagacatatt agtaagagaa gccggggaaa acctattttt ggcagtgaag ataactggat
    22021 tattcaaaaa tatgcacatc cgtatcatgc cgaagcaaga aaaaccttac agaagactag
    22081 caaaaatttc agagtctcaa ccagaattcc aacaaagcaa tgtccaagga acattagtcg
    22141 ggttctttac gcctgaatta ttccaaggag ttgctgcagc gggatttcac ctgcatttta
    22201 tcgatgatac acatacattt ggcggacatg taatggattt tgaagtggct gaaggaaaag
    22261 ttgagattag ccgcatctcg tcccttgtac agcattttcc agttgatgat ccgacttttg
    22321 ttcacgaaac gattgattat gctgatttag cagcagaaat ccaacaagca gaatagaaaa
    22381 agacagcgag atgcgttcaa ggtgattaac ctgacgtatc tcgttttttt gaggagaaaa
    22441 cctagcgttt acaaaaaaac tgagctataa tcttctccat agatcaaaaa ggagcggttt
    22501 ttatcatgtg gacagaaaaa cagaaaaaag atgcatacga aattttatta ttacagcaac
    22561 aaggattatt agaagcagaa gataactggt tagcaaattt agcaaattca tctgcattgt
    22621 tgaatgagac tttgcctgag actgtctttg caggatatta tttattcgat ggtaatgaat
    22681 tgattttagg accgttccaa gggcgagttt cttgcacacg tatcaagatg ggaaaaggag
    22741 tttgtggaga atcagctgaa aaacaagaaa cattgatcgt tgatgatgtc aaaacccatg
    22801 caaattacat ttcctgcgat tcagccgcac aatcagaaat cgtagtgcca atggtaaaag
    22861 aaggtaattt aatcggtgtt ttagatatcg attgcggagt aacgaaaggg tatgatgaaa
    22921 tcgatcaagc atttctagaa aaatatgtag atcttttatt agcaaatttt aatataaatg
    22981 ctttttcatt tattaataac taaaatcgtc tattgaaaca aaaaagaaga gaaatcaaga
    23041 ataaaaagag ccggaaagac agtgggcatt tctgtttttc ggttttttta tttttttata
    23101 aaatcacaag tgttattgat tttcataatc atattttaat gttattatca ttttaaataa
    23161 tgaaaaaaca ttaaggatgg atgaaatatg aataacgaaa ctatcatttt aggaaaaaaa
    23221 tatatttgcc aacctattgg cttgaaacaa aaagtgattg gagaagtaat cagtaaatta
    23281 gaaaattgtg ttgtgatctg cgtagacgaa tatcatgacg tcgatcgcaa tgaaatatta
    23341 gaaaaatgcg gaaaagtagt ggcaaaatat actcacgttt atgagttgga aacaacagaa
    23401 tatctatata actatagcaa caaatatgaa catactttag tttcataaag atttaaataa
    23461 agtgccttca aaagcgagaa gaccaagctt ttgaaggcac tttatttagt tggcagtgat
    23521 tgttcatgat ttttgattta ttcaaaaggt tcgcctagag aatctgtctc tgtaacgagg
    23581 cctggtggag aaactcgata aaaacctata ctgccagaac tgtcctattt tcggatagac
    23641 aaactagtag ttttaaaaag atcatccgat ccaatcattt caaaaaatgc aaaactgata
    23701 tcaggatcat ttagctcagg cttgttatcg taaagcattt gtaaaggttg catttagtta
    23761 ggagtaaacg cgaaactatc atcattattt gctgaagaag atgaagaagc taattcagag
    23821 tttgtttgaa ataaaggaga cgtgctgaca gtttctttac cctgtttttt gcagaggtag
    23881 aaacttttat atcggaagtt tctaccggag aatttttctc atttgtagaa attgttgaat
    23941 cgatactatt cgaaatttgc gtatctgaag gtatgttctt tgatgcagaa gtcccacaag
    24001 cagtaagtaa caataaattt gataaactga taaacagaat tcttttcatt atgtacacac
    24061 tccttatagt atcgtttagc atacacctat gctcagtatt aggcaaaatc aatgcaaaac
    24121 tatcaagtct tttatagtat ctattagtct tagtaataca tatatggaga gggtgatatc
    24181 ccagttcaaa ccagatacat tggttaccat ctgtattaac gattatgcct ctaaacgctt
    24241 tttttatgaa aaaatttata agtgaaaacg taggtatgat attatctaga ggtaattttt
    24301 ttgggtattt aaatagttgc taggaggata gcttttggat ttttacagta attttatctt
    24361 aataatagca atattattac ttctaaatat ttggtttttt gataagagta gaaatgcagg
    24421 gataggtttt agaaccaaac gaagtacgtc ttctgaaaaa aagtgggtat attcacagac
    24481 tattttttac ggaggggtta tttcaatcag tttactttct tcaacacttt attcttttaa
    24541 tgttatagac gtttccatgt caaattttat atctattatt ggaatcctta tttctgcaat
    24601 tattacacaa ctacttcttg tgtttgaaga gaagtcaaaa aataattaaa gcaattatgc
    24661 cttttagtac aggaattatt acttcttaca aatgtatagc gaatgtgaag cctgctgttt
    24721 taattgttct agaaaaactt ttgagtgaac aattatttta tgttaggatg aattaatttc
    24781 tctagagttt ata
//
LOCUS       DAYYFD010000039        24348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000039 DAYYFD010000000
VERSION     DAYYFD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.039"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..596)
                     /locus_tag="ACHA25_001485"
     CDS             complement(<1..596)
                     /locus_tag="ACHA25_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8762780.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEET"
     gene            974..1957
                     /locus_tag="ACHA25_001486"
     CDS             974..1957
                     /locus_tag="ACHA25_001486"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8762781.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(2325..4463)
                     /locus_tag="ACHA25_001487"
     CDS             complement(2325..4463)
                     /locus_tag="ACHA25_001487"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8762782.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(4502..6088)
                     /locus_tag="ACHA25_001488"
     CDS             complement(4502..6088)
                     /locus_tag="ACHA25_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8762783.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(6078..7298)
                     /locus_tag="ACHA25_001489"
     CDS             complement(6078..7298)
                     /locus_tag="ACHA25_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762784.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(7310..8116)
                     /locus_tag="ACHA25_001490"
     CDS             complement(7310..8116)
                     /locus_tag="ACHA25_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762785.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(8326..9606)
                     /locus_tag="ACHA25_001491"
     CDS             complement(8326..9606)
                     /locus_tag="ACHA25_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8762786.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(9650..11683)
                     /locus_tag="ACHA25_001492"
     CDS             complement(9650..11683)
                     /locus_tag="ACHA25_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762787.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(11717..12568)
                     /gene="rfbD"
                     /locus_tag="ACHA25_001493"
     CDS             complement(11717..12568)
                     /gene="rfbD"
                     /locus_tag="ACHA25_001493"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8762788.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(12666..13694)
                     /gene="rfbB"
                     /locus_tag="ACHA25_001494"
     CDS             complement(12666..13694)
                     /gene="rfbB"
                     /locus_tag="ACHA25_001494"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8762789.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(13716..14288)
                     /gene="rfbC"
                     /locus_tag="ACHA25_001495"
     CDS             complement(13716..14288)
                     /gene="rfbC"
                     /locus_tag="ACHA25_001495"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8762790.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(14302..15168)
                     /gene="rfbA"
                     /locus_tag="ACHA25_001496"
     CDS             complement(14302..15168)
                     /gene="rfbA"
                     /locus_tag="ACHA25_001496"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8762791.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(15268..15975)
                     /locus_tag="ACHA25_001497"
     CDS             complement(15268..15975)
                     /locus_tag="ACHA25_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8762792.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(15980..16807)
                     /locus_tag="ACHA25_001498"
     CDS             complement(15980..16807)
                     /locus_tag="ACHA25_001498"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8762793.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(16807..17607)
                     /locus_tag="ACHA25_001499"
     CDS             complement(16807..17607)
                     /locus_tag="ACHA25_001499"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8762794.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(17600..18739)
                     /locus_tag="ACHA25_001500"
     CDS             complement(17600..18739)
                     /locus_tag="ACHA25_001500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8762795.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(18944..20137)
                     /locus_tag="ACHA25_001501"
     CDS             complement(18944..20137)
                     /locus_tag="ACHA25_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8762796.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(20216..20422)
                     /locus_tag="ACHA25_001502"
     CDS             complement(20216..20422)
                     /locus_tag="ACHA25_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8762797.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(20415..21557)
                     /locus_tag="ACHA25_001503"
     CDS             complement(20415..21557)
                     /locus_tag="ACHA25_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8762798.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            22069..22704
                     /locus_tag="ACHA25_001504"
     CDS             22069..22704
                     /locus_tag="ACHA25_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8762799.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            22840..22932
                     /locus_tag="ACHA25_001505"
                     /pseudo
     CDS             22840..22932
                     /locus_tag="ACHA25_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            23046..23681
                     /locus_tag="ACHA25_001506"
     CDS             23046..23681
                     /locus_tag="ACHA25_001506"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8762800.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            23788..>24348
                     /gene="mef(H)"
                     /locus_tag="ACHA25_001507"
                     /gene_synonym="mefH"
     CDS             23788..>24348
                     /gene="mef(H)"
                     /locus_tag="ACHA25_001507"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8762801.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIF"
BASE COUNT         7424 a         4948 c         4176 g         7800 t
ORIGIN      
        1 tcagtttctt cgatatgaga agcaagatag acaatatcga tgtctttaat catatttttg
       61 atgtttgcat aatagttatc aagaatgact gcttcgactt tatgtttgtt gtttttcttt
      121 gaaccaaaat tttggattgc tggtgcgaca tccttttcta acccaataat gacgaccttt
      181 ttatctcgac ttacgcgtaa ataaagttta tatacgagaa tacgatagat acttaagaaa
      241 agcgtaccga gaacaaatga aagtaagata acaagacgtg gaaaagcaaa ccagcggccc
      301 gcaaaagtca ctaccatgat ccctagtgtc atcaatacct gaccgatgac tgtgacaaac
      361 acgatgtcac tgatcattcg attataaaaa acatatgcac ccaacaaaat gttcaacgca
      421 ataaaaatca ctgaaataat aattgctgag tgttggaaag tttctaaatt tcgtaatggg
      481 atctctccac gaaacttcag taagaaacta ctatatattg ctgcattgta tacaataaca
      541 tccattaata taatgaatat ccgtcgtgca gcactccatt ctccattttt attcatatat
      601 acactcctaa tcacaaaata atatctctat tatcttacca tagtcacctt taaaaaccta
      661 gtttataagc aaattgtaat ggaatttaaa aatgtctaat aaaaagcttt atacacctct
      721 aatcaagaca aggactctaa attacaaatc tccagatgaa tcgattttca ctaatgttgg
      781 taaattaaag aagtcgaagt atttgctgct ttttttatcc actcataaag tgaaccttta
      841 tatttttcag agaatccatc ttcccaaagc gctgtctagt ccataagaat acctgtatta
      901 cgtgaagaat cgaaaaaatt gaaatcgatt tttaatgaca ttatgcttat agaagaaagt
      961 gaggtaatat cttatgaata aacaagagtt gcatgatttt attggttttc gacaaccaaa
     1021 tatatgtcaa attgtcgcat ataaggatgg ggtagaagtt tatacagatg aatggaatgg
     1081 ttataaaaaa gacgatactt gtcatatcat gtcagcaacg aaaagtattg tatctttgct
     1141 gattggcatt gctttagagc aaggattaat caaaagtatc gatgataaag tattagatta
     1201 ttttccagag tataaagtga aacgcgggga gaaaactatt tacgctatta caatcaagca
     1261 cttgctaaca atgaaagcac cctacaaatg taaaggcgat ccatggacaa aagtctgttc
     1321 aagtgaagat tggacaaaaa catcactaga tttattggga ggaagaaaag gtataaccgg
     1381 cgaattcaac tatcaaactg tctgtttaca catattaact ggcttgttgt atcagacaag
     1441 taagctaaca actgttgatt ttgccaataa gtatctattt gatcctatag ggataccaaa
     1501 ccatgtcaat tattatgctg aaacagctga agaacataag cagtttacaa tggataagtc
     1561 tcctaaaacc aatgtctggt tttgtgatcc actaaatatc gggacggcag gatatggttt
     1621 gtgtttatcg gcaagtgatc tggcaaaaat tggtgtatta tgcctaaata aagggatgta
     1681 taaaggaaaa caaatcgtac cacccatgtg gataaacgag atgttcaaac ctagaaccag
     1741 tgctttagaa gacttacaaa atatgagata tggctattta tggtgggtat tagattctga
     1801 gaacgaaata tattcagcaa ttggtaatag tggaaatgtt ctttatattg acgctaagaa
     1861 caatctggtt atctccatca cttcttattt taaacccatg ataattgata gagtggcttt
     1921 tgtagaggaa taccttaaac catttatttt aaaatagcct atattaaaat aaaatatcga
     1981 ttctttgaca gctagggttc gtgaaacttt ggctatgatt ttgtatctat tggcgctctg
     2041 agcatgaata agagcgtggg acaaaagtaa gaaacacttt tgtctcattc tttaaaaacg
     2101 aataaacggt gggagcagaa gcaacttctt cttaaataag ccgaaattca caaaaatttg
     2161 aaaagcaatt ttcgtgaatt tcttcttatt tctcggagtt aaacacttct gtcccaacct
     2221 cgacttattt ttttgagtcg gcaccttctg cctttttttg acaaaaaact ataaaaagcc
     2281 gttatgtgcg agcgaatcac aacataacag ctttttatta tagtttaatc tggtctcaac
     2341 gaaaagtcgc cgattccttc ttttgttaaa ttatcattgt aaaatggatc atgcgcaata
     2401 tatgctggcc atttatcttg catttttttg atttcaccag caaaacgtct ttgtttttct
     2461 ggagtatctt cataacctct tgatttagat tcgaaatggt acaattctgc ttgatgtgca
     2521 tagacattgt aacgacccag ttcataaact ttcaagcaca agtcaacatc gttgaatgct
     2581 acttctaatg tttcatcaaa cccgttaaca gcattgaagt cagctgcctt gaccatcata
     2641 caagctgcag tgactgctaa gtaattgacg tcaatgacca atcggccgaa ataaccacaa
     2701 tgtgttcgat catagttatt caatgcatgc cctgcaacac caccgattcc taataatact
     2761 cctgcatgct gcgttgtatc atctggataa aacagttttg ctcctacaca gccgattcga
     2821 tcaaactgtg cataagaaac catcaccgtc atccaatctg gctcaatcac ttctgtatcg
     2881 ttattcaaga ataaaaagta tttgccgttt gctttttctg ctgctaaatt gttaattcga
     2941 gaataattga atggaatgtc aatcaattct acgataaagc ggtctttcag ctgatgcttg
     3001 tattctgcaa ataattcttg catttttgga tctgtacttc cgttatccgc aataatgatt
     3061 tcataatttg aataagacgt tttttcgatg atcgaatcca cacaagtttt tagatcttca
     3121 tagccattct tcgttggaat gatcacactg accaaatcct cttgcaagac gtcataagca
     3181 atctcataaa atccagaaat tctgccagga cgtacgcttc ctttgatgtt tcttctagac
     3241 agggcatctg tcaatgcttt cactccggaa tcatagatat aacttttggc ttcgccatta
     3301 cttgcagttg atcctgggat cgtccgccaa tgatacaaga ctcggtcaat gtggtaaata
     3361 tgatctgcag ggatttgttc tgtcacacga aggaccaaat cataatcttg agaaccttca
     3421 tagcctttac ggaaaccgcc aagttcttta acaatgcttg ttcgatatac gcctaagtga
     3481 gagatataat tatttcccat caatgtatca ggcgaccagt ctgctttgaa atgcggatca
     3541 aagcgattgc catctgcatc gattttatct tcgtcactgt agatcacatc cagttctgga
     3601 tgtacgttta gcacttttgc cacttcatat aatgcaaatg gcggtaattc atcatcatta
     3661 tctaagagtg ctataaattc tccttctgct atttccaatg cagaattagt tgccagactg
     3721 atgtgtccat tttcttttcg gaaaacgact ttgattctat catcttttgc ttgataggat
     3781 tccagacact ttcggatagc tggatcagta gaagcatcat cactgataca tagttcccaa
     3841 tgatcatacg tttgatccag taccgaatca atgcattttt cgagccattt gatttcaacg
     3901 ttatagacag gcataagtat ggaaatcaat ggacggtacg caaatccttg tgcttctttc
     3961 ctttgctctt tcaaatctgg tttctcatga cgactgatcc actctgcata gtcgatcgat
     4021 cctggcttca attccaattt gacccgacgg atcgcttttt ttacgccatg agtccttgca
     4081 taatttatcc cttttttcag taatcttttt tttctttccc atgaagattc aatgccgtca
     4141 tcataagggt atttaccgtt taatttcaca gctatgccat tttgatgtct cgctgtttgg
     4201 aagtctaaga tggcttttcc tctcattttc cctgataaac gtaccttaaa accgctttgg
     4261 gtcttaacat caagattata taaatgattg atatccaatc ttacgacatg tgttgcttca
     4321 gcaatcacat tttctttttc tacctttatc gtagggattt ctttggtcac ttcatcaatt
     4381 gcccagccca caataagcaa atcgccagtg gcccgttcac gagtgatttg atcgataaat
     4441 acggcaattt cttttttttg cacgattcgt ttactcctta tctttataag ttaatcatta
     4501 tttacatttc aatgatccat gattggccat tttgagctac tgcttccact aagaatgaac
     4561 gttcttgctc gcaaaggatt cgtaactcaa atcccgcttc tgttcctttt gcgagctgtt
     4621 ctgcttgatt gacttcttct cgtgaaacag gaattgcttc gaaaaatggc gattggtcag
     4681 ctgataactt ataggacaag ggctggcgat ccgaattagc gattccccat ccgatgattt
     4741 ttaatatttt tgtttctgca tcccagattt tctcgtcaat acatatagaa agttcttttt
     4801 cttggatttt tttcgaagtt tctttcttac gtcgtcctag tctatgtttt attgtccatt
     4861 catcactttc caacaatgtg caatagcgat ttgtggttgt ttttaacgta tggtctagtc
     4921 ttgtattttc ctcaagaaga tgatgatttt ctctttctaa ttcgtcattt ttctcagaaa
     4981 gctgctttgt gacttcagcc atgggacgaa tcgtttccaa caattctttc attgttggtg
     5041 tcaattcccc gataaagcga tagtgacaat ggatagtcac ttctttccca gaaatatccg
     5101 ttagaacgaa ctctggtaat tctttcccat caaacaaata acaatcattt actgttttga
     5161 ctacagcatt cgaatcgata aatgttagtt tttctccggc agcttctgcg ctgaattcga
     5221 taaagctagg ttgttgcgcg aagcgaaaca ccattctttc tgttgtttct gatacgggga
     5281 aacttagtgt ctcattttcc cctgtgaaat tattgaatgg tatttgttgg attgtttcat
     5341 cttgtttcgt ttgctgggtt acttctaaca cttttacata attagaattt tgtggctctg
     5401 caatggaact ttgtgctact ggatgtttca tcaatgagaa aaagtattgg tagacttcac
     5461 caaatggtcg cattttgaaa tcatagcgta cagcttccgg caattcttca taagttgatt
     5521 ttaattctgt atcacctaca gctaagtaca aataatcttc aatattaatg aataatcccg
     5581 ctttttcaaa aactttttgg aagccatcat gagtataaaa agaattatgg gtttcatcta
     5641 ggagaccata agaagcccac ggcaattggt tattgaacaa atcgatcatg acagaattat
     5701 gcgccaagtt agggaatgta atcaggatct ctccttcttc attcaaaaat tctctgactt
     5761 tcttcaatgt ttttccagga tctaccaaat gttctaaaac atccgcaaac aagacgtaat
     5821 caaatgtctg tccagcataa tagtttgccc actcaaaact ttcgatatca ccataaaatc
     5881 catcttgagc aaattcgctg acaaaatcga acaattcctt atctaactca acaatgctca
     5941 cttcacattg ttttgctccg attaaatgtt tcgttaatcg gccgtttccg ggaccaaact
     6001 ctaaaacttt acttcctggt ttgatctgtg cggcaatttt tcccacactg ctttgttcat
     6061 ctaggtccat ttcaaaatca tatttcattg ctatgagtcc cttcttccca tttatggtct
     6121 aagaccacga tgccttctcc gatataagaa ccgctgacaa acaagttcat gtactttgtc
     6181 ttatatacca aaggaaccgt tgcattttct tcaaagaccg caacgtcaaa ataatattca
     6241 ccagctaaca aacctaactt ttcatactcc agataaaaga tattctctcc ttttttccaa
     6301 ggaatcttga taccatctag tagagtatta agtccgcata cataaaaatt gtcgactgtt
     6361 cgtagcgcaa ttcctaaaac cggatttttg attgtatcat cttttacttc ataagttact
     6421 ttaacgtata gtttttctcc ttgttccagc atatccaccg gatttttgtc tttatccaac
     6481 aattccgctt tcgtcacgtc gacgatatct ggagcggcga accgttctac ttggttttta
     6541 ttccgatcca ctgttttaac tgattttttc tttaaaaagt tttcataatt cgtcgttact
     6601 tccatcgtat cgccatattc gacgacttct ccgtttttca accaatagga acgatcacaa
     6661 aaacgacgaa tcgaactgat atcatgagaa acgaataaaa tcgtctttcc cgagttcttg
     6721 atttctgtaa atttttccat acattttagt tgaaattcaa gatcgcctac tgccaatgct
     6781 tcatccacaa tcaaaatatc gggattcaca ttgattgcta cagcaaaagc tagacgaacg
     6841 aacatcccgc ttgaataagt ttttacaggt tgataaatat ggtctccgat gtcagcaaat
     6901 tcaatgatat catcgaccat ttcatcgact tcttctcttg aaaatcctag aaccattcca
     6961 tttaaataga tattttcata gccagaatac tcaggattga atcctgatcc tagttctagc
     7021 aaagctgaga tttttccttc gatttccatc gtgccggatg ttggtgtcag tactcccgta
     7081 atgattttca acattgtaga ttttcccgat ccgttttctc ccacgaaacc gatcatctca
     7141 ccttttttta tttctacatt cacgtttttc aacgcataaa atacatcatg ataggatttc
     7201 ttaaaaggat tgagggcttc acgaaatcga tccgaaggtt ttgcgtacat attataagat
     7261 tttgtgatat cttttagtct aattgcatat tctgtcatag ctattctcct tacagcacat
     7321 ctgaaaaatg tggtttcaat ctacggaaca ctgcagagcc gattacgaac attactaacg
     7381 tgaaaaccca gaaatacaaa gtatatgttc catgctccca gaaccaagct tcacttaaaa
     7441 atgactcacg ataaccttga acaacatagt acagcggatt taatttcaat atgcttacaa
     7501 gacctgaatc aaactgatta gggctccata atatcggtaa tgtccacatg atcaactgca
     7561 gcacgatcgt aattaattgc atgatatcag gtaaaaatgg ttggatcgat gccgtcagcc
     7621 aagtcaatcc tgtcaagaaa gcacacatgc aaaataaata ataaatcagc tgtaaagaca
     7681 ttgctgaagg atatatacca tttataagcg cgacagctat gccaatcaga atgaagaata
     7741 agtgggtgta cagattggat aggattttcg tcgtgggcaa gatacgaata ttgaatacga
     7801 ctttctttac taaataagta tattcccgaa aaacaaccgt agttgatgca aatgcatctg
     7861 agaagaagaa ccagggaatc atcccggtaa ctaaatatac gataaatggg tattttgcat
     7921 cgatcatttg tgctttcagt ccaacaccaa ataccagcca ataaatcaat acagtaaata
     7981 gcggattgat aaacgcccat aagattccta aaaacgatcc tgcatatctt gatttgaagt
     8041 cattgatcga aaactgtagc aataattttc tgttctgata aatgtccttc ataaataaat
     8101 acaaatcttt aaacatttgt gttcctccaa gcgattttca gtgaattaca gtcaagtgcc
     8161 attttaccat atttttggta ccgagctgcg ttcttatttc tttcttaatt ttctatgata
     8221 caagattttc tctaacgttt ctattttaca gaagatgcta gacaattgca ataaaccaaa
     8281 aaaggctgcg acggttgtca cagcctctct cgtattttgt gcctatcaat gatagcgttg
     8341 gatcctggaa gaatgagcgc tatctggttg ttcctcataa gatgtatcat cataagtcat
     8401 atcgtcatat gcctcttcca taacatagaa ttcctccgca acttctggtt catcatatcg
     8461 atgaacggcc atagccaaca ggatataaat aatcggaacc catgggtatg aagtcaatgt
     8521 atcctccata aatgactgca tcatcaccgc aaaaaaggcc cagataaaag tcatctgccc
     8581 gttggataaa tctgctttca acgttaagcg accaataaaa aggataagca gcaaagcagc
     8641 aatcccaaat cctatcaatc catagatatt caaaatagaa ttataactat tgtggtttcc
     8701 taaaaattgg tttcctcgtt ggaagataaa tgttttcatt cctagtaaaa tttgttcaga
     8761 tttttctcct agcgtctgat agaatttatg ccaaatatct tctcgaccgg taaacaaatt
     8821 gactttttca gagacagccg ccagataaga aatcggcaaa gctaaaagaa tcgtaaccgt
     8881 gtaagcaata aatggcgcac gaatgacacg catcaacgat ttaggtaaaa gatccaatat
     8941 cgcaaaaaca atcaacgcga ctaatgcacc tctagattgg cagacccaaa tcccgagaaa
     9001 agctgcaaca actgctactg caccgatgat tcgtacatac catcgttcaa aagaactggc
     9061 aaatccactg atcaataaag caagagtcat caaagaactt ccgattatat tcgtattgat
     9121 ccaaatatca gaaagcgaat tgcctggagc ccagatgctt tgtccttctg gtacttggat
     9181 ctctgtaaat acacgatata acgtgattcc gaggaagacc gaaaagacaa tcagataaat
     9241 accgcgatcc cattttgtcc actcgatcca tttaattact aaacaaaata aaataatcaa
     9301 aggaaacagt aagtctccaa ataaagactc atttcttaat tcccgaaagt aagaaaagaa
     9361 aatgaaaagg gcaagcgcaa cacctagata aagaaaatcg acgccttttg ctttataaac
     9421 atttaaaatg agcaaaatag ccacactaaa agcgataagt aatgaactat tgttatatat
     9481 aaacaaagtt gctggaaaac ctaaccctgc tgtccaaaca aaaaagacta gcgtcagtaa
     9541 caaaagcaag aaaccgacac tcattttgtt gacaaatttt tgtacgattg cttgaattat
     9601 ctccactctc ctttctacta acatttcagg attattctca gctagcctat tactgtacga
     9661 ctagctgatt ttcctgagtt tgtgtttgtg gtacatctgg ataatccgga tattctactt
     9721 taaaaatcat ataaccgtct ttatccggtc catatagctg cgtgaactgc gcttcatata
     9781 attggttgta atcagttaat ggtcgtttac tgatcacata gtcgatccct aatttttctg
     9841 cgtcttcaaa attcaacgtg accgaaaaca tatctggacg atctaaaatg aactttgtat
     9901 cttccttagc gataaaagct cttgtatgag cataccgatt ataatacttc tcgtattctt
     9961 ctttcggatc gattttcttc catgcagcta tatctggata aaagcgtact gtattgaagg
    10021 atttcactcc caatgttggg gtgaagttat ataattcatc ttcagacaac caaactttac
    10081 caggatcttg ttgatcgatt ttttggattt cttttgccag tgttttttca aaaatcgctc
    10141 ctgtaccttc tacgactgga ttgacgaaaa agccagatac gataactaag gcggcaaaag
    10201 cgaggtaaaa caagcgtctc cagccaaata atgcaaaagc taaaatcact gttcccaaca
    10261 cagcaactgc gatggtttca aaatccgaaa aataattatc cattttcgaa tgcgtaatcg
    10321 catagaaata agaccatgca tttactgcca acaaaatgaa tttataccaa ataggaataa
    10381 catcttctct ccaaatataa gcgataaacc agatcgataa taaacaagcc gcaaaactaa
    10441 aagtaaggat ggctcttaat ggaggaacat aagacagcag agtgatttct gctagccatt
    10501 ttggcaaccc aacggtaatc caaaagatag aaaacagaca aaaaatcatc aaaatacgtc
    10561 ccagtaactt ttgttcgcta tcttttttgc caaataacac aaatggactt gctaggaata
    10621 cagttggaaa aaagtgatag aacagggcca cttcagagtt attcgaatag ttgatatctt
    10681 caaaaggaat tttccaattt gtaaggaaag aaaaaaattc gccaatcgtc cactttcctc
    10741 cagtacttac ccgttttcct ggatataagg tattcaaaga agctaacaat gcgtcttttg
    10801 acgttagcca gaaatgaacc atgactccgc caataaataa aactgcccct ccaatcaaca
    10861 ctacatccaa cagatcccat gaaactttct tgctatagta aataagcagg ccaacaaaat
    10921 aaaaaatcag caagtacgcc agcatcactt gatgggctgg gtaaatcact aaaataaacc
    10981 caataccgaa aataaccacc aaaagcatca tcaacgttcg tagccatttt ttatcatgat
    11041 tgtagaaata atgataaaaa gcagtcatca gtcccaaagt aaagaaaatc aaatcgccca
    11101 catgttgcat gaaccaccac tggactgctg gagaaaatgt cagcgcaaat gcaccgatta
    11161 gcgatagagc tttttttctc ttcgttaata tcatcagtac ttcgaatcca agcaagacca
    11221 tgacgactaa tttcatcgcc caataaaagg aaagtcctcg atctcttccg aggaaaaaga
    11281 atccccagtt gaatggtttt ccgataaccg taatatcctt tacaggagaa ttatacgcaa
    11341 tgatcatgtt ttgtccgttt aatgaataat cctcgttcac caacggaaag tcctcttcag
    11401 cttgtgagag gtaaaaaggt gtctgcacca gccattcgtc tgaccgcact tcccgattct
    11461 gtccaaaaat cacatcagtt tttttcccat cgtctctttg cgacacatat ttatcccaaa
    11521 caccaagtga acttccgttt aaattaaaag taacgatgac cacgatgact aaaacgccaa
    11581 taagatagcg gagttttaga aacaggtcaa gcaccaaatt cgaatctgcc tggttcgctt
    11641 ttggcttttc ttttcgtcgt gcacttcttg tttgtttttc catatcatgc tccaaattct
    11701 atttgataaa aggaaattac ttattcattt ttttggcagc ttccagcatt tgcgcaagtg
    11761 cttcttgcca agttggtatg acaaatccta actcttctgt ctttttcagg ctcatgactg
    11821 aatactgcgg acgtgttgct ttttgcggaa attcttctga cgtaacaggt gcaacttcta
    11881 catctgtatc tttcaatatt tcttttgcga attcatacca agaacagcta ttttcgttcg
    11941 acaaatgata aataccaaat ggtgcttttt cttcgatgac atataccata aattctgcca
    12001 aagtacgtgt ccaagttggt cgcccaaact ggtcatttac tacagtcagt ttgtcgcggg
    12061 tttccgctag gcgttgcatc gtgaagacga aattgtgtcc atagatcccg aaaacccaag
    12121 aagttcgtat gatataatag tcttctaaaa tctcttctac tgctttttcg cctaataatt
    12181 tcgtacgacc atattcattt agtggattcg tttggtcgtc tacttcgtat tctacatttt
    12241 tgttttttcc atcgaaaaca tagtctgtac tgatatagac gaattgagca ttcacagctt
    12301 ttgctgcaag tgctacattt cttgtaccat caacattgat cttctcatct aattcttttg
    12361 cttcttcttc tgctttatct actgctgtgt aagctgcaca atggaaaata acatcaggtt
    12421 ttaattctgt gatgtaagcc attgttttct cggcatctgt gatatccaag tcttttgcat
    12481 ctgttgaaac atattgcaga tttttttcat ctaataaatg gcgcaattct gtacctagtt
    12541 gcccatttcc tccagttaat aaaaacatat gattatccgc tccttgattt ttattttaaa
    12601 aaagagacag gcgagcgcct gcctcacatg tgtttttcca ttaatacagt gacattttct
    12661 ctgtattatt gtccattttt ggcatatgct gcttcaacgg cctctttttc ttttttccac
    12721 cagtcttcat gttcttcgta ccatttgatc gtgtcagcca acccatcacg gaagttcgta
    12781 aactcaggct tccatcctaa ctcttcccgt aattttgtcg aatcgattgc ataacgcaag
    12841 tcgtgtcctg cacggtcgtt tacatggtcg tacgcatctt ttggttgacc catcatttct
    12901 aagatcaatt ccatcacggt cttattatct tcttcaccgt ctgcaccgat cagataggtt
    12961 tcgccaattc tgcctttcgt caaaatcagc cacactgctg aagaatgatc attcgtatgg
    13021 atccagtcac gaacattctt tccttcgcca tataattttg gacgaatacc gcttaagata
    13081 ttcgttactt ggcgaggaat aaatttttca atatgctgat aaggaccata gttgtttgaa
    13141 cagtttgaaa tggttgcacg taagccaaaa gaacgcaccc atgcttttac taacagatct
    13201 gaaccagctt ttgtagaaga atatggactt gaaggattat atggtgtttc cgcagtgaat
    13261 ttttctcctt cgccttcccc atgtcctggc aggtcttcac gtaatggaag atcgccgtaa
    13321 acctcgtcag tagatacgtg atgatagcgt acatcatact tgcggcaagc ttctaaaagt
    13381 gtatacgtac caatgatatt cgtttgtaca aatgggaaag gatcttttaa tgaattatcg
    13441 ttatgggatt ctgccgcgta atgaacaact gcgtccgctt cttttactag acggtccact
    13501 aattctgcat ctgcaacatc tcctacgacc aattctacgc gatcgctagg aagtccagcc
    13561 aagttctctt tgtttcctgc ataagtcagt ttatccaaaa cagtgacatg cacttctgga
    13621 tgattgttta ccacgtaatg aacgaaatta gagccgatga atccggcacc tccggtaaca
    13681 atgatgtttt tcataagaaa atttctcctt aattcttaga tttctccgta aacaaatgga
    13741 ttttctgctt caaattcttt taatgtcgga tgacggtgat ctttttccga cataatcgct
    13801 cttgatggat caattggcca atcaatcgct aaatctggat catcaaatgc gatgccccca
    13861 tctgcttctg cattatagta attatcacac ttataaagga agttcacatt tggtgtcaaa
    13921 gtaacgaatc catgggcata tcctttaggt acaagcaact gtcgatgatt atgttcagat
    13981 aagataaatc cgtcccattg accataagtc gggctacctt tgcggatatc cactactaca
    14041 tctaaaactg ctccagtgac tacccgaatc aattttgtct gtgcagcttc tcctttttgg
    14101 aaatgaagcc cccgaaggac accagcttca gctgatagtg aatggttgtc ttgaacaaag
    14161 tcatatttca atccatgttc tgcaaatttt gcttttgaat aactttctgt gaagaaaccg
    14221 cgatgatcgc caaagacgtc catttctacg attttgacat ctgttaattt tgtatctgtt
    14281 actttcatct gtaaggactc cttattcagc ggccaaattc aataaatatt gaccgtattg
    14341 attttttttc aatggctgtg caagggccag caattgttcc ttatcgatgt agcccatacg
    14401 gtaagcaatt tcttccaaac aagccacttt taagttctga cgtttttcaa ttgtttcgat
    14461 aaaggtagat gcttcaagca atgattcatg tgttcctgta tcaagccaag caaatccgcg
    14521 tcccataagt tctacggata gttttccttt atccaaatat gctttattga catccgtaat
    14581 ctctagttct ccacgttctg aaggtttgat acctttagcg atagaaatta cctcattgtc
    14641 ataaaaataa aggccagtta cagcgtaatt tgattttggc tgagcaggct tttcttcaat
    14701 cgacaaagct tgcatattct catcaaattc tactacacca aagcgttctg gatcattgac
    14761 atgatagcca aatactgtcg cgcctgtatc ttttgaagct gctcgttgca acattttaga
    14821 cagaccgcca ccatagtaaa tattgtcccc taataccaag cagacactat catctccaat
    14881 aaattcttct ccaatgataa acgcttgtgc caatccatct ggactttctt gtactgcata
    14941 ttcgatctgc agtcctaaat catttccgtt tccaaataat tccttgaaac gtggtgtatc
    15001 ttgtggcgtt gaaataatca gaatttcttt gattcccgcc aacatcagag tagacattgg
    15061 ataataaatc attggtttgt cataaatcgg catcaattgt ttcgatgttg cctttgttaa
    15121 agggtacaga cgtgttccgc tgcccccagc aagaatgatt cccttcatgg atatctgctc
    15181 tcctttttga tttcaaattt cactttgtta ttatctcatc aattgttata tttgtcatgg
    15241 aaatattaag gatttaaaca tcttttatca acgtttaaac cattttcgtt tgatcaacgc
    15301 cgtaatcaga tgaacacgcc aagaaatttt ttgccgtaat ttagaatgtg tttcaatctc
    15361 actcgcgttt aaatcatgtc ttcggtacag agttaacggt tctcttagga acccagtctt
    15421 ctttcctccc agcaaaccaa tccacatatc atgcatcggc acttctgacg ggatcggcaa
    15481 aatatcgggt tttaaagaag accggaaagc cataccagca ccaatatagt gacttcttat
    15541 cacattatta agccatcctt ctttggaatt tcgaaacgaa aaatacgaat catggatctc
    15601 ttgcagctcg ctgtccacaa tgattaaatc agaaactact actagctgat ccggattctg
    15661 ctcaaaatag tcagtgatct ttttcacttt tgtctccaac caaacatcat cttggtctgc
    15721 caaaaagatt agatctcctt tcgtctgctg gatcgcataa ccaaaattag cgatcaatcc
    15781 ttgacgaggc cccgaaaaaa gctggattcg cttatctttt tctgcgtatt cagttaatat
    15841 atcccatgta tcatctactg acccatcatc agaaatgact acttcatctt ctgctgaaag
    15901 ctgagataaa atactatcta actgctctct taaatatctg cctccattaa aggtagctat
    15961 acaaaccgaa cgcacgaatt cacaccttcc aattcttaac atatgagcgc aaagtcatcc
    16021 gccagatatg ccggtttttg acggtcaaga actgtttagc tgttcttaac aataattgtc
    16081 gtcggtgact ggataaatgc ctttgatcat attcatgata aaatcgattt tctgcttgta
    16141 aaatagaacg gtatcgatta cttgaaactt tttcatagtc taaaaccgaa agatcatgtt
    16201 ccattcgtac aggaaggatc tccacagttt tttccaactg atccactttc caaaacagcc
    16261 aatgatccaa aaaatctaaa gaaaaagcag gattaaaccc gccaatcttt tttagaaatt
    16321 tgacagacca ggcaacacct gaattgattg ccatgattcg ttcagctgtt gggccttcaa
    16381 cttctactgc ttgagctgta cgattgatat aacgatctga atatagggga gaaacttgtc
    16441 ggctcccaga aaaaatcatc ggaactgcag caacacattc atctgtaaag gttactttac
    16501 gcaatagttc aaaataatcg tcgactagcc tcgtatcctg atccagagtg acgagacaac
    16561 gtgttccttc atctgcttga gacaacgcaa aattataagc caccgctaaa ccaggatttt
    16621 gcggatcatg atgataaacg atattttctt tttcaaacag ctcattttgc tgcctttgag
    16681 gactattatc gtaaataata agctgaattt gcttttcgct aagtgctttg cttaatggtc
    16741 cataagctgg agattgactg aatagttgct gataaagcac aacgactact aatatttctg
    16801 gtttcatcag aaaaaacgcc acccccactt attgaaaaat ttcatcatag aagtcaaaaa
    16861 cacgcgaaac agttttgcat ttcgatgcgc acccttcccg taaaaatgga taactgcata
    16921 aaagggtgtg tacaataact tgtaacctgc tttttcaaaa ctcaaacaca agtcgttgtc
    16981 ttcaaagtac atgaagaaac gctcatcaaa cccctttatc tctttaaatt tatcaatatc
    17041 agctaccata aaactacctg agatcatccg tacataacta ttagcatcag taggcaatcc
    17101 tcggcattca aacacagcca gtcgtttgtc aaactttttt ttgacccatt tgaaaggcac
    17161 aaagcgcaaa acgtaatcaa aaacagccag ctgctcacgg atgaggtgct gagttgttcc
    17221 atcttcatta agaatcttag gtgctaacat cgcacattcc ggatgttctt tcaataacaa
    17281 gaccaatgct gtaactgtct cttcatccac taaaatatct gggttacaaa taatgccata
    17341 gcgagaatcg ctagctatca gattgtgatt atgtccataa ccaaatccgg aattttcatc
    17401 atgaaaatag atatcgatgt agggatattt cctatactct gttagtcttt ttttgtactc
    17461 gggaagtgaa ttgttgtcaa aaattcggat ttttactgct gtttcatctt ttacagttga
    17521 aataaaatta tctagtgtcc gaaaaatttt atcactgttt gccgtcacaa tactaataag
    17581 gatcggctgg tttaatttgt tattcatcta tatgacggtt ccatttcttc aaatatttct
    17641 gttttacttg cgtacggtac tctttattcc ctaaaaatcg caatgttttt aaaataaact
    17701 gatgtttctc attgatcaga ccaatcaatt caaataataa aattgcagca aacaacatgg
    17761 caataatcaa tatgatcgtt ccgattcttc cagtataatt catgaccata gctgtgaaag
    17821 aaaacatcaa agccatcgca taaatcgtca ggacagcacc tttatgagta aagcctaaac
    17881 tgattagtcg gtgatgaaga tgttttttat ctgctgtaga aaaagaaaca cggtttgctt
    17941 ttctcctaat gatagcaaaa aacgtgtctg ttaccggcac tcccatgacg atcagcaaag
    18001 aaatcgatga aacaaaagtc acgttcttca gcccttgtag tgaaaggacg gccatcataa
    18061 accctaggaa tagagctcct gtatcgccta aataaatctt tgccggataa aaattgtaag
    18121 gaaaaaaacc gataatactt gctacaagac agaaaataac aactggaata tacactgtag
    18181 aagcatggag gaaaaaatat ccaatgattc ctatcgtaat taaaccgatc aatgaaatcc
    18241 ctgccgccag cccatccaaa ccgtcaatca gattgaccgc attcgtgata ccgaagatcc
    18301 aaaaaatcgt tacgggaaaa ctaagccagc ctagttggat atatccaata aaaggaagcg
    18361 tgaaggaatc aatacgtata tctgctacaa aataaataat aagagaagca atcaagatcc
    18421 ctatactttt ttgtttaggt gtcagctcat atttatcatc gagcactcct gtgactatga
    18481 ttactaaaga agcaatcaag atcggtacaa tatacgattg agggataatt ttacggaata
    18541 agaataaagt cgatataaca aatgaaataa agataggcag tccgcccgct gaaggcattg
    18601 gaactttatt tattcttctt tcatttggtt tgtcgaccat acctgtttgt actgaaatga
    18661 ttttgaatac aggtgttaat atcagtgata ataaaattgt cagaaaaaat cttataacca
    18721 tactaaaaga aacaagcata tgggcaccta ctttaattaa gagtcataat tattataaat
    18781 tatatatgtc tttttgacta tctttttata tcttctcaaa tgtccgcaaa gccttatttt
    18841 atcggctcta cgggcatttt gcttttgtgg taaacctcgc atatctaggt ctatcttctt
    18901 atattttccc tgtcaatcgt ggttaaaatc gtggtaaata cttctatgcg attagacgct
    18961 gaacctctga ttttgcggta tctatggacg catgagcgta ccagttcatt gtgatactga
    19021 tgtttgaatg ccccatgata tactgtaaat ctttcgggtt catgttcttg cttgccagcc
    19081 ttgtgcagaa tgtatggcgt agcgtatgcg gtgtaatatg tggcaaggga ttgtccttgt
    19141 ggtgcttgtt gtatttcttt accatacgga taaataaggc gttataatca atggcaactt
    19201 ttggattacc tttcggattg ataaacacaa aaccgctgtg tccgtctacc tctatcgact
    19261 ttatctttgg tcggctgttg ataacccgtt gaaatgcctg tatcgtttct ttgcttaatg
    19321 gcacttgcct tatcccgctt ttcgtcttag gcgtttcaat ataatagccc tgttccttac
    19381 tctttagtaa ctggtggtcg ataatcacaa cttcattctt gaaatcaatg tcggctactg
    19441 tcagtccgca cagttccgag atacgaagtc ctgtctttaa cagtatcagc acatcatcat
    19501 aatgcttgtg atacacgttg tccgtcttaa taaatgacaa taaggcttgc tcctgttcct
    19561 ctgtcaatgc gactttctct ttggtatcat tttctatgac ttcacttagc ttgaaatcaa
    19621 aagggttttt cctcacataa tcatcttgta tggctatgta gaatgacgct tttaacgagc
    19681 gtttatggtt gttaatggta ttgtaggaaa agcctttgtc tttcatgcgt aacgcccatt
    19741 ctttagcgtc agagggtttt atcgtatcaa tgctcctagc acctaacttg tcctctttta
    19801 ataaccgcat gagttgttcc cgttgtttct gtgtgctttt cttcacattt gtcctctgtg
    19861 cgttctgttt ggcatagagt tggcaaagcg tcattttctt gcctgtgctg tcgataccgt
    19921 cctcaatatc ccgtcttatc tgctgttcca gttctcgaag tgagggtttt tcccgttttc
    19981 cctttggtgt cgggtctgtg ggtgtcaatc tccaagcata cacatatttt gtgtttccca
    20041 atgcgtctac atatttatat aagtattttc cgtctgttcg ttggctctct ccagtatgca
    20101 ggatacgacc tttgttatcc cgtctttttt ctttcatggt gtctgctcct ttccttgttg
    20161 gaaagagcct tgatatgact tgtgttcatc ataacacata caaggctcat ttgcattaga
    20221 ttgcgtccaa tttatcaata accttttcaa actgtcggcg tttaatctgt atgcggttgc
    20281 cattcataat gagccagcca gcgtccttgt tttcctctgc caatcgtctt aacttgtttt
    20341 ctccaatgcg gaaatacttt gacgcttctt caatggtaag ggtgtatttt tcccatacgg
    20401 gaatatcctg tgtattactc ataaaacacc ccctcacttt cgattacgtc tgatatgtcc
    20461 gtacacatct gttccacaag catttcctgt ttttcagaaa ggtgggtaca ttcatcaatc
    20521 attttcatgt agtttgaacc tgtcagcctg tcgatttcct ctatcatcac gattgacggc
    20581 atgacctgtc tttccagcca ttgcagagtg cgttccatat tgacaggtac agggttcatt
    20641 tcaaaaggta aactgtcatg ggaaatgaat atctcccaca atggatagtc aggaaattcc
    20701 acaaaatcgg taatgagata aaacaccagc ccatgagggt tatacgtcaa taacagttct
    20761 tcaactgcct gtaccgcctt tttgtcacgc agacgaattt caaaacggtt gataatatcg
    20821 gtatgtttct ttttcgttgc ctgttccttt tgtttttcat acaggcagaa atattttgtg
    20881 cttgcctttg agccgatata aagggtactt gctaaattac ggttttttcc gcttaatttt
    20941 ccaccttgta cattttcata gtttttacag cgacattccg caccaccatt tctgtatttt
    21001 tcagatagca caggaatatc cagcagaccg cacatatcat tgattgccaa gtcaaaacgt
    21061 cggataacac cgccacagtc aagacagcgg tttaagaatg aataccagtc cctgttctgt
    21121 gcctgtaaga catattccat gttgcgtgag cctgcccctt ttaattctaa aaatacgccc
    21181 atgtgttcat catcagaagc catgaccttg atttccccgt aagcataatg ctccttatag
    21241 ccataatacc catagtcata atggataaaa taatctgctt tcatgccaag cacctttcgg
    21301 ataacttcca atgcgtcagt cgttggaaaa cggacagaca gataatcaac gagcagataa
    21361 aaaggcttat cgcagatata gtaattcaga caacgcagga taatgttctg tgtttccttg
    21421 ctggcgttag cttttccgtt ctcaaagcgg ttgatactat atcttgatac atgggtaaga
    21481 cttgcaagtt ctccttgtga aagaccgctg gcaagacgtt tgttcttcat ttcaagtgca
    21541 aattctaaat tgttcattgt attcctccaa tcccacaata gccttgaaat acctgtaaac
    21601 cacctttcaa aaagtgtgat atgtactatc ttcttaaaac ctctcattac tactcacagg
    21661 tttgggggtg tatggttttt gacttgtctt ttgtgaaata gtgcccccct gttagataac
    21721 gggggcacag acaaggctcg caggctcgcc agccgacaaa gcatttcttc cccttgacac
    21781 aaggtgtcat ggcggtaagc ctgagaggct tccgccaccg tgtcagaaat actttgtcag
    21841 caagttctgt tttgcctttt ctaacaagtt ccatttcaac cgatattcat atctacggat
    21901 ttaatcagat aggtatttct ttttgaatgt ttttatattt cattctattg tgcttattcg
    21961 attacaagaa catagtaatt cttatatgct tatttgtcaa tatctttttt tgcctttaca
    22021 aatcatatta agcatgatag acttataata gcgaagatat aacgagatat ggagggttat
    22081 gcaatgaaaa taagccattt aggaaataat atacagacca taagaaaatt taggggaatg
    22141 aaacaacagg aacttgcgga caaaatcggt atcaatatgc agagcctttc caagattgaa
    22201 agagggttga actatcctgc ctatgaaacg ctggaaaaga taatggaagt gctggacgtt
    22261 acgcccaatg aattgttgtc gggagaatgg aagtatgtca atcaatctga aaaggaagtc
    22321 tgtcagtttt taagagccga agaacgtcta aatgcagaac tgaaacatgg acactatgat
    22381 aacttctttg acagcgagga agaatggctg gaatatgagt tagaaaagtt acgggaatat
    22441 attacagact atatcaacgg gaaaagcatt gaagcgtccg acctttaccc aatcaaagaa
    22501 tttatccagc atttgaagtt tcaaaaatta ttagaccgct atgatgattt atacagtatg
    22561 gatatgtttg gggaaagcat agagggacat aagtataaga caccttatca agtcgtgaaa
    22621 atgataaacc cgaactcaaa agaagatatg gaactgttac gagaagtatt aaggaataac
    22681 aattttgatg atgaggacga gtaacttttt tcctcttgcg agaagataca gcaaacaaaa
    22741 agcccgatca agagtgcaaa gcaccaccaa tggtggcaaa gctcttgaca gggctttttc
    22801 ttttctgttg tgggcaatca agaggaagaa agggataaaa tctgatttgt ggtaaatcag
    22861 gcaaaggcaa cgcaggtatc acagtatgaa ttgacaccgc ataaggaaag caactggaag
    22921 attgtagaat aaatgaaaaa ggcttgcatt ctgcattttt ataaaattgc aagtctttat
    22981 attgacaagc ataaaatact aagatatact ttaatcagac caacggtttg tattggaggt
    23041 aagttatggc acgaaataaa tatccagaag aaacagtaaa actcatttta gatgtttcta
    23101 caaaaatgtt ttgtgaaaaa ggatatgata acacatcatt gcaagatatt atcaatgaaa
    23161 caaaactttc taaaggtgct atttatcatc atttcgattc aaaggaagat attttaaaag
    23221 caatttttca aagaattgga aatgaaaatg cagtaatatt tgctaaaatt agagataacg
    23281 atagtttaaa cggtttagaa aagttaagag aaatatttaa gacagcggta ttcaattcta
    23341 accaaagcgt tttattgaca gtttctcctc aactattaaa caatcctcgt tttcttgcta
    23401 tgcaaataga acaaatgtat gaaattgtag tacctgaatt tgttgaaccc gttttagaac
    23461 aaggtatcaa ggataagtct ttggagattg aaaattcaca tgaaattgca gaagttatca
    23521 tggtattaag taatgtatgg ctcaacccat tagttgaaat gacggataaa gagggtatgg
    23581 aaaaacgttg caatacattc aatgcccttt taaatggaat aggcatagac acattattag
    23641 acgaagaaac aatttctgca tttatacagt tttgtaaata actgtatttg ttatccctat
    23701 acgatagact gctctataaa agagcagttt atttaaacca ttatataaac cgacggtctg
    23761 tttgattaaa taaaaaggag agattttatg caagcagaaa aaaaactttt taatcgtgat
    23821 tttatacttg ttgttatagg acagataatt tctttatttg gaaacgctat cctacgattt
    23881 gcattaccgt actatttgtt aaaagaaact ggttcatcag ctttgtttgg tattgctaca
    23941 gcctgctctt ttttacctat ggttattttg tcgctggtag gtggtattat tgctgacagg
    24001 acaaataaaa gaaacattat ggtcatacta gattttgtaa cagcaatcat aattttagta
    24061 ttttatttgt cattgggaaa aattcctatc atacctttgt ttattgttgc tttgatgtta
    24121 ctttatggta tttctggtgc atatcaacca gcggtacaag caagtattcc tctattggta
    24181 tcatcagata aacttaccgt cggtaatgct ataataaatc aagtaaatac tttagcaaac
    24241 tttatcggac cagctataag cggagtagtt tttcaatttt atggtatcaa tcccatttta
    24301 attgttagta ttatatgttt tactttttca gccattatgg aaattttt
//
LOCUS       DAYYFD010000040        24259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000040 DAYYFD010000000
VERSION     DAYYFD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.040"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHA25_001508"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHA25_001508"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8762802.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHA25_001509"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHA25_001509"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8762803.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHA25_001510"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHA25_001510"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8762804.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHA25_001511"
     CDS             complement(3373..4680)
                     /locus_tag="ACHA25_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8762805.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHA25_001512"
     CDS             complement(4683..5264)
                     /locus_tag="ACHA25_001512"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8762806.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHA25_001513"
     CDS             complement(5836..7128)
                     /locus_tag="ACHA25_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8762807.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHA25_001514"
     CDS             complement(7125..7748)
                     /locus_tag="ACHA25_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762808.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHA25_001515"
     CDS             complement(7751..8587)
                     /locus_tag="ACHA25_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762809.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHA25_001516"
     CDS             complement(8604..9491)
                     /locus_tag="ACHA25_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762810.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHA25_001517"
     CDS             complement(9507..10790)
                     /locus_tag="ACHA25_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8762811.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHA25_001518"
     CDS             complement(11473..12156)
                     /locus_tag="ACHA25_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8762812.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHA25_001519"
     CDS             complement(12174..12791)
                     /locus_tag="ACHA25_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8762813.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHA25_001520"
     CDS             complement(12801..13625)
                     /locus_tag="ACHA25_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8762814.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHA25_001521"
     CDS             complement(13618..14178)
                     /locus_tag="ACHA25_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762815.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHA25_001522"
     CDS             14352..15308
                     /locus_tag="ACHA25_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8762816.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHA25_001523"
     CDS             complement(15613..16569)
                     /locus_tag="ACHA25_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8762817.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHA25_001524"
     CDS             16673..17275
                     /locus_tag="ACHA25_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8762818.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHA25_001525"
     CDS             complement(17363..18862)
                     /locus_tag="ACHA25_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8762819.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHA25_001526"
     CDS             complement(18878..19402)
                     /locus_tag="ACHA25_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8762820.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHA25_001527"
     CDS             19700..20449
                     /locus_tag="ACHA25_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8762821.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHA25_001528"
     CDS             20440..21225
                     /locus_tag="ACHA25_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8762822.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHA25_001529"
     CDS             21194..21910
                     /locus_tag="ACHA25_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8762823.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHA25_001530"
     CDS             complement(22012..23031)
                     /locus_tag="ACHA25_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8762824.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHA25_001531"
     CDS             complement(23050..24051)
                     /locus_tag="ACHA25_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8762825.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5221 c         4225 g         7625 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgatttt
//
LOCUS       DAYYFD010000041        23831 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000041 DAYYFD010000000
VERSION     DAYYFD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.041"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACHA25_001532"
     CDS             complement(55..789)
                     /locus_tag="ACHA25_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8762826.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACHA25_001533"
     CDS             1116..1526
                     /locus_tag="ACHA25_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8762827.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACHA25_001534"
     CDS             1519..2220
                     /locus_tag="ACHA25_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8762828.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACHA25_001535"
     CDS             2220..3806
                     /locus_tag="ACHA25_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762829.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACHA25_001536"
     CDS             3825..4046
                     /locus_tag="ACHA25_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8762830.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACHA25_001537"
     CDS             4043..4804
                     /locus_tag="ACHA25_001537"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8762831.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACHA25_001538"
     CDS             5184..5693
                     /locus_tag="ACHA25_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8762832.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACHA25_001539"
     CDS             complement(5768..6307)
                     /locus_tag="ACHA25_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8762833.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHA25_001540"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHA25_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8762834.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACHA25_001541"
     CDS             7320..8171
                     /locus_tag="ACHA25_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8762835.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACHA25_001542"
     CDS             8199..8834
                     /locus_tag="ACHA25_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8762836.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACHA25_001543"
     CDS             8854..9627
                     /locus_tag="ACHA25_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8762837.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACHA25_001544"
     CDS             10035..10832
                     /locus_tag="ACHA25_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8762838.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACHA25_001545"
     CDS             10810..11223
                     /locus_tag="ACHA25_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8762839.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACHA25_001546"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACHA25_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8762840.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACHA25_001547"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACHA25_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8762841.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACHA25_001548"
     CDS             16000..16560
                     /locus_tag="ACHA25_001548"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8762842.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHA25_001549"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHA25_001549"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8762843.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACHA25_001550"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACHA25_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8762844.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACHA25_001551"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACHA25_001551"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8762845.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA25_001552"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA25_001552"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8762846.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA25_001553"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA25_001553"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8762847.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHA25_001554"
     CDS             21907..22485
                     /locus_tag="ACHA25_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8762848.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHA25_001555"
     CDS             22537..23019
                     /locus_tag="ACHA25_001555"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8762849.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHA25_001556"
     CDS             23136..23510
                     /locus_tag="ACHA25_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762850.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23623..>23831)
                     /locus_tag="ACHA25_001557"
     CDS             complement(23623..>23831)
                     /locus_tag="ACHA25_001557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762851.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7781 a         4286 c         5115 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 cagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatggggt attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DAYYFD010000042        23449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000042 DAYYFD010000000
VERSION     DAYYFD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.042"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            206..1534
                     /locus_tag="ACHA25_001558"
     CDS             206..1534
                     /locus_tag="ACHA25_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8762852.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            1434..2189
                     /locus_tag="ACHA25_001559"
     CDS             1434..2189
                     /locus_tag="ACHA25_001559"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8762853.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            2193..3404
                     /locus_tag="ACHA25_001560"
     CDS             2193..3404
                     /locus_tag="ACHA25_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8762854.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            3415..3726
                     /locus_tag="ACHA25_001561"
     CDS             3415..3726
                     /locus_tag="ACHA25_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8762855.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            3723..4061
                     /locus_tag="ACHA25_001562"
     CDS             3723..4061
                     /locus_tag="ACHA25_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8762856.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            4054..4485
                     /locus_tag="ACHA25_001563"
     CDS             4054..4485
                     /locus_tag="ACHA25_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8762857.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            4482..4823
                     /locus_tag="ACHA25_001564"
     CDS             4482..4823
                     /locus_tag="ACHA25_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762858.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            4826..5416
                     /locus_tag="ACHA25_001565"
     CDS             4826..5416
                     /locus_tag="ACHA25_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8762859.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            5426..5806
                     /locus_tag="ACHA25_001566"
     CDS             5426..5806
                     /locus_tag="ACHA25_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762860.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            6514..8574
                     /locus_tag="ACHA25_001567"
     CDS             6514..8574
                     /locus_tag="ACHA25_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8762861.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            8645..9046
                     /locus_tag="ACHA25_001568"
     CDS             8645..9046
                     /locus_tag="ACHA25_001568"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762862.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            9123..9815
                     /locus_tag="ACHA25_001569"
     CDS             9123..9815
                     /locus_tag="ACHA25_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8762863.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            9812..13702
                     /locus_tag="ACHA25_001570"
     CDS             9812..13702
                     /locus_tag="ACHA25_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8762864.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            13712..13879
                     /locus_tag="ACHA25_001571"
     CDS             13712..13879
                     /locus_tag="ACHA25_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762865.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            13866..14273
                     /locus_tag="ACHA25_001572"
     CDS             13866..14273
                     /locus_tag="ACHA25_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM8762866.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            14274..14693
                     /locus_tag="ACHA25_001573"
     CDS             14274..14693
                     /locus_tag="ACHA25_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8762867.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            14683..15591
                     /locus_tag="ACHA25_001574"
     CDS             14683..15591
                     /locus_tag="ACHA25_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8762868.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            15707..16744
                     /locus_tag="ACHA25_001575"
     CDS             15707..16744
                     /locus_tag="ACHA25_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8762869.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(16805..17110)
                     /locus_tag="ACHA25_001576"
     CDS             complement(16805..17110)
                     /locus_tag="ACHA25_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762870.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            17405..17578
                     /locus_tag="ACHA25_001577"
     CDS             17405..17578
                     /locus_tag="ACHA25_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8762871.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            17639..19192
                     /locus_tag="ACHA25_001578"
     CDS             17639..19192
                     /locus_tag="ACHA25_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8762872.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            19240..19605
                     /locus_tag="ACHA25_001579"
     CDS             19240..19605
                     /locus_tag="ACHA25_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8762873.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            19607..21157
                     /locus_tag="ACHA25_001580"
     CDS             19607..21157
                     /locus_tag="ACHA25_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8762874.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            21280..>23449
                     /locus_tag="ACHA25_001581"
     CDS             21280..>23449
                     /locus_tag="ACHA25_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762875.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVG"
BASE COUNT         7516 a         4198 c         5358 g         6377 t
ORIGIN      
        1 caccaagtag ttcattcttt ccttatcaaa gaaaatcctt aacacctgac gaaaaatcga
       61 aatgtttaga ggaagtaaat acctttttaa ataatgcaat tgagataatt ttgtcaagag
      121 gaagtcggtt ttatatctaa tgtgaacaca gcatctacaa atcgtaggtg cttttttgat
      181 actcaaattt tgaaaggagc aatcaatggg cataataaaa tcactattca aatcacgcga
      241 taaaccaaca aatcaaacca tcatgaatcc ataccgattt atgtttggcg gctcatcagc
      301 tggtaaaagc gtgaccgaac aaacagctat gcagatgacg gctgtgtact catgtgtgcg
      361 cgtgctatca gaagccgtgg caggattgcc attacacttt taccaatacc gagatgctgg
      421 cggcaaagat aaagcaatga aacattcgtt gtatttcttg ctgcatgatg aaccaaattc
      481 ggaaatgaca tcatttgtat ttcgtgaaac cttaatgact cacctgctac tttggggtaa
      541 cgcctatgcg caaattatcc gaaatggcaa aggtgaagtt gtagctttgt atcctttaat
      601 gccagataag atgacggtta atcgcgatga caacaaggaa atctattatt tatatcaagc
      661 tgatgtcggg caagtcagac tttcaaaagc tgacgtgctt cacattcctg gccttggctt
      721 tgatggcttg gttggttatt caccaattgc catggctaag aatgctatcg ggcttgcgat
      781 tgcaacagag gaatatggtt ctaaattttt tgccaatggt gccaatcctg gtggtgtcct
      841 agaacatcct ggaactttga aagacccagg aaagcttcgt gaaagttgga atgcgacatt
      901 tggtggcagt tcaaatgcgg ccaaggttgc agtacttgaa gaaggcatga agtatacgcc
      961 tatttccatg tcaccagaac aagcccagtt ccttgaaaca cgtaagtttc aactggaaga
     1021 aatcgcacgg atattccgag tgccccttca catggttggc gacttagacc acgcgacctt
     1081 ctcaaacgtt gaacatttga gtttggattt tgtgaagttc tcattatcac catggctcaa
     1141 tcgttgggaa caagcactta ataaatcact gttaagtcgc gatgaaaaag aaaagtattt
     1201 cataaagttt aatgtggatg gcttattgcg tggtgactat gccacacgtc aacaaggcta
     1261 tgcaaccggt attcaaaatg ggttcctatc tcctgatgat gtgcgtgaac ttgaggactt
     1321 gaacccaatc cctggtggtg ctggtgcgaa atattacttc aatggcaata tgctgccaat
     1381 tgaacaagcc ggggcagctt atggcgtaaa caaagaagaa agcgaggaac aagatgaaga
     1441 agttttggaa gtggaagaat caaacggttc tgaatcaaga caaccaagag gaaatcctag
     1501 agagaaccct ctacctcaac ggaccaatcg ctgaggagag ctggtttgat gatgaagtca
     1561 caccgcagat tttcaaagat gagttaaatt caggtggagg taacgttaca gtctggatta
     1621 attcgggtgg tggcgattgt gtcgctgccg caagtattta caacatgctt gctgactaca
     1681 aaggtaaagt cacagtcaag attgacggca tcgccgcttc ggccgcaagt gttattgcaa
     1741 tggcaggtga caaggtactc atgagtcctg tggcgatggt gatgattcac aatccgtcag
     1801 cggttgcgtt tggcgaaaaa gttgatatgg agaaagccat tgccatgctt gatgaagtga
     1861 aagcaagtat tatcaacgcc tatgaacgta aaacgaattt atcgcgtgtg caactggcac
     1921 atatgatgga tgcggagacg tggatggacg caaataaagc tgttcaccta ggatttgccg
     1981 atgaaatcat cggtgagatt gacccggata ttccggttaa taattcgatg tactcggaag
     2041 tcaaagtaac taattcactg acttcagcga ttgctaagaa gtgtcgtatt gaaccacctg
     2101 aaccaaagga acgagaagaa attaataaca ctatcccagc cgattcgtta atgagtcggc
     2161 ttaatttaat gaaaaattgg aggaactaac ccatgaacaa aattcaagaa ttgatggaaa
     2221 aacgtgcaca agcttggaac ggagcaaagg cttttgtaga agcaaagcaa gataaagatg
     2281 gcttgatgtc tgaagaagat gccaagactt atgcggaaat ggaaacaaaa gtgaagaact
     2341 ttacgatgga aattgaacgt atgcagtcaa tggaagcgat ggatcgtgaa cttgaaaagc
     2401 caatgagcca accattaact tcgcaaccta tgactactga aaataaaatg gaacctaaga
     2461 aaacaggacg tgcttcagct gaatataaag aaggtgtact tgaagccttg cgctcaaact
     2521 tccgacatgt ttcaaatgtt cttcaagaag gtgtggatac ggcaggcggt tatttggtgc
     2581 cagaagaata tgactcacgt ttgattgacg gcatgacttc tgaaaacatc atgcgtacac
     2641 ttgcgacaac aattacgaca agtggtgaac acaagattaa tattgcaggt tctaaaccag
     2701 ccgcttcatg gattgaagaa ggtggcgagt tgagctttgg ggatgcaacg ttcgatcaaa
     2761 tcttgcttga tgctcacaaa cttcatgtgg caatcaaggt gactgatgaa ttgctttatg
     2821 acaacgcctt taaccttgaa aactatatca tcgaccaatt caccaaagcc ttagcaaacg
     2881 cggaagaaga tgcgttcttg aatggtgacg gtgttggtaa accactcggt atttttgcag
     2941 caacaggtgg cggacaagtt gccattacaa ctaatactca aagtgcagtt acggcagatg
     3001 atgtggtgaa ccttgtgtat gctttgaaac gtccatatcg taaaaacgcg aagtttatca
     3061 tgaacgacca aaccatcagc ttgcttcgta agttgaaaga caacaatggt gcctacatgt
     3121 ggcagccagc tttggtagca ggtgaacctg accgtctgct cggttatcca gtttatactt
     3181 cggagtttgt tccaaccgtt aaggcaggtg cgccagtcat tgcttttggt gatttcagct
     3241 actataacat tggcgaccgt ggcgtgcgtt cattcgacca gttgcgtgaa ctctttgcag
     3301 gtaatggcat ggttggtttc ttagctaaag aacgtgtgga cggtaaactt gtgttacctg
     3361 aagcagtgca aatcttgaag atgaaagcat ctgcctcatc ttaaggagta gcctatgagt
     3421 gacttactaa ataaggtcaa ggcaaacctc atacttgagc ataacgaaga tgatgagctg
     3481 ttggaacgat tgattctgac cgctctttct tatgctgaga gctatcaaca tattgagtct
     3541 ggctactatt cagaacatga aatgccgcca accactgaac aggcggttat tgtgttagcc
     3601 agtaactttt acgaaagccg tgatggttca actggtggct tctttgcgga taacgtcaat
     3661 gccagtaagc aagtctggga aacggtcaat ctgttactca ggctcgatag gcggtggcaa
     3721 gtatgagttt tggaaagatg aatcaacgaa tacaaatcgt taaagtgacg aaccaaaagg
     3781 atagcgctgg attttcagtg aaacaagatg aagttcttgc agctgtccat gcatacaaag
     3841 aggaaaagaa tgcgactgag aaatgggtaa acatggcgac ttttagttca gcgacctgcc
     3901 tgtttcggat tcgttatatt cctaatctag aggttacgac agagatggtt gtcatttcag
     3961 atcagaaaag gtatcagatt gtgtcggtgg agaatgtccg aaatcgcaat atgtaccttg
     4021 aaatgctgac tactgaaatg gaggtgagtg cggatggcta aagcattgat gaagatgcca
     4081 gaagatttct tgttaaaggt ttcaaaacta gaaaacaaga cagatgaaat catccccaag
     4141 gttttagaaa gtggtgctga agtggtggaa ggcaaagtcc gaagtaactt aactgccgtt
     4201 gttggctcaa atacaaaagt accaagtcgt tcaactggtg aacttgaatc agcccttggt
     4261 atcacacaag ctcgtcaaga taaaagcggc aactggaaca tcaaggttgg ttttgatgag
     4321 cctcgaatcg atggcagttc aaatgctaag attgcgaaca ttctcgaata tggaaaacac
     4381 ggtcagccag caaagccatt cttgaaacct gcaaaatcac aaagtcgcaa gtcatgcatt
     4441 gaaacaatga aatcaaaaat ggaaagtgag gtaaacggca tttgaacatt ttagaggaat
     4501 tgaacagtct ttttgccaca cttcatattc ctgttgaaac gggcgagttt agtgataaag
     4561 cgccagacaa gtattgtgtg ctgacaccac tcgctgacag gttcgatgtt tacggggata
     4621 actttccaac cgttgatgta caggaagttc gcatttcctt gtttgtgaaa ggcaactatc
     4681 tgaacacgca aaaacaactg gttcagaact tgttgaaaca cgagtttacg attactgaac
     4741 gattatttgt aggatttgaa aaagatacaa attactatca tgtggcgatt gatgtcgcca
     4801 agcattatga aatggaggtc tgaaaatggc aacgattgga ttggataaat tatattatgc
     4861 accgattaca gagtcggcaa ctactggcga agaaacatac ggcacacccg ctatgcttgc
     4921 gaaagcaatc tcagctgagc tttcggtgga acttgctgaa gcaactttat acgccgatga
     4981 tggtgcatca gaagtagtga aagaatttaa aaatggaaag ttaacgctcg gtgttgatga
     5041 tattggacgt gctgtggcgg cagtgcttgt tggtgcaacc gtggatcaaa acggtgtctt
     5101 aatttcgaca accgaagatg gtggcacacc agttgcgatt ggtttccgtg ctcgtaaatc
     5161 gaatggcaaa tacaaatatt tgtggttgta ccgagtgaag tttgccgtgc ccagcacaag
     5221 cctagcaaca aaaggtgacg acattacatt ctcaacacca acaattgagg gtacagtcat
     5281 gcgtcgtaac aagttagatg ctagtggtaa gcatccatgg aaagcagaag ttgatgaaaa
     5341 cgatacggga gttgaacaaa caactatcaa tggatggtac acgagtgttt atgaaccaga
     5401 tttcacagca gactaggagg caacaatgag tgaagaacgc agtgccaaga ttagccttgg
     5461 cggagatgaa tatgaactga ttttgacaac aaaagccacg aaagaaatcg ccagtcacta
     5521 cggtgggctt gataaccttg gtgagaaatt gttgaaatcc gagaactttg aattggcgct
     5581 tgatgaaatc atctggttga ttacgctgtt ggcgaatcag tccatcaaga tttacaactt
     5641 gaagaacaca gaaaagaaag atgaactcac gactgaatat gtggagttga tgacgagtcc
     5701 gttgcagttg gctgaatata aatcagcaat cactgaagca atgttcaagg gcacagctcg
     5761 caatatcgaa agtgaaacag attcaaaaaa caaagcaggc gagtaagtga tgaagaaaca
     5821 tttactcgcc tgtattacta tgggacagtt caaatgggta tgacgtcaga tgatttctgg
     5881 tactgctcgc ttggactgtt tttggattta tgggagtgtc ataagcaatt tactgggatt
     5941 agcaagccga aagttgagtt attcattgat gatgtgatac cgagtggaat ttaagggaag
     6001 gtggtgattt atttggcaga taattttggt ttaaagattg gcgttgaagg tgaaaaggag
     6061 tttaagaact cgttgcgtga aatcaaccaa tcattcaagg tgctaggttc agaaatgaaa
     6121 ctggtatcat ccgaatttga taaaaacgat aaaagcatca gctcactgtc tgctcgcaac
     6181 caagtcttaa acaaatcgat tgatgcccag aaagaaaaaa tcaccaccct tgaaaatgct
     6241 ttgaagaacg ctagtgacag ttttggtgaa aacgataagc gtactcagaa ttggacagtg
     6301 caattaaaca atgcaaaagc ccaactcaat aatatggagc gtgaagttgc taacaacgag
     6361 aaggcaattg acgccatggg tactgaagaa gttgatgctg ctaagaaaac tgatgagctt
     6421 ggcaacgaac tcaaggatac aggtgacgag gctgaaaagt ctggaagtaa atttgaaaag
     6481 ttcggtggcg ttctaaaagg tatcggggta gcaatgggta cagtggcact tgctgctggc
     6541 gctgctgccg tggagctagg aaaacaagtg gtatctgctt acgcggacta tgaacaatta
     6601 gtcggtgggg tggatactct tttcaaggat gcgtcatcca ccgttcaaaa gaatgccgac
     6661 aatgctttca aaactgccgg aatgtcagcc aatgaataca tggaaactgt cacaggtttc
     6721 tcagctgcct tgatttcatc tctcggtggt gataccaaga aagccgcagg agttgcggat
     6781 caagctgtta ctgatatgag cgataatgca aacaagatgg ggacggatat tgagtccatc
     6841 cagaacgcct atcaaggttt cgccaagcaa aattacggga tgctggacaa cttgaaatta
     6901 ggttttgggg gcacgaagtc cgaaatggaa cgtttgctcc aagaggctga gaagatttct
     6961 ggtgtcaaat atgatatctc gaacttcgct gatgtgactg aagcgattca cgttattcaa
     7021 accgaaatgg gcatcactgg tacaaccgcc aaggaagcga gtgagacgat aagtggttcg
     7081 attgacggta tgaagtctgc aatctcaaac ttgatggctg gacttggtaa cagcagcgct
     7141 gacattggta tgttgattgg caatgtggtg gaacaatttc aaaacgtcat caagaatatt
     7201 ctaccggtca ttcaaaatat cgtgtcggct ttaccggcag tggtggatgc cattattcaa
     7261 gcattggggc agttattgcc aacactctta gaagcagtaa cctcattatt ctcacaagtg
     7321 cttgaaacga tacttggctt acttcccaca cttattccag tggtggttca agccgtttta
     7381 acgattgttc aaacacttat tgagaactta cctttactga ttgacgctgc ctttcaactt
     7441 gtgacaacac ttatccaagg actaggcgaa gcgctgccag aacttattcc aattacagta
     7501 caggcaatca taacgattgt gaacggcttg attgaaaact tgccattact gttggatgct
     7561 gctttacaac ttatcatggg acttgcccaa ggacttatca cagcacttcc aatactgatt
     7621 gaaagtctgc cacagattat tacaggaatc gtgaacttta ttatcagtgc tattccacaa
     7681 atcattcaga ctggtattca acttttgaca tcacttgtga cagctttgcc ggatatcatc
     7741 aatgcgattg ttgcagctat tccagtgatt attcaaaata tcatcaatgc cgtgattggg
     7801 gcaatccctc agttgattga cgcaggtatt cagttacttg tggcattgat tggtgcgtta
     7861 ccgcaaatta tcacaacgat tgcgaatgcc atgccgcaaa ttatcaacgg aataacagga
     7921 actttggtaa acaacatcga caaaatcatc aatgctggtg tgaagttgct tgtggcatta
     7981 gttcaaaact taccagcaat catttctgcc attgtcaaag cagtaccgca aatcatcaca
     8041 gggcttgcga atgctttcgg tgagtatgtt tcaactatgg gaactatcgg gaagaacttg
     8101 attacaggat tgtggaatgg tatcgctgat gctggaagtt ggttacgcaa caagatttca
     8161 ggattctttg gcggtgtagt agacaacatc aaggacttct ttggtattca ttcaccatca
     8221 acactgtttc gtgatgaaat cgggaagtac atggcacaag ggattggcgt aggttttgat
     8281 aaggaaatga ctggtgtttc taaaacgatg cagaaagcta ttccaacaaa actttatatg
     8341 ccaagctttg atattgatac cgatatccac gctgccgttg atagcacatt ctcgatgctt
     8401 gatttaggtg ctaaggttga tgccttaaca aacatcacga gtgaaatgtt gccaacgcta
     8461 cttaaagcaa tggacattaa agtcgtcttg aatgatggaa cattggtcgg caaacttgct
     8521 ccagaaattg ataagagctt ggcgacgttg aatcgtagac gtgtagcata ttagtgttgg
     8581 attttcacat tttgactgta tactataact aacgagtgtt tactgaagag atggtggtaa
     8641 tcatatgttg aagcataaat ttaaagcaaa gaaacctgca ctgataggat atattctact
     8701 gcttgctatt ggattatttt taacggcagt aagatggtat agcggcataa atcatggttt
     8761 tgtattcatc aacaatcaga ttaattctca catttcaaac ttcgcattaa gctttctttt
     8821 ctatttggtg ataggctatc tttggttgct tatgggaacg aatttaaggg ttcttactgc
     8881 gatgggagct ggtctgattg ttgcaaatat tgtatgtgaa acgatgatga cttttctgaa
     8941 tacaccagat ctaatcgatg caatatatgg aatttccggc gtgctggttg tatacgcgta
     9001 tttagcgatt accaagaaaa tagggctgga agaaacaacc gaataggatg atagattata
     9061 aactttagga ttagacattg ttgtctggtc cttttttagt tatttagatg ggcggtggtc
     9121 aattgaacgc atttatttta gacggaacaa ttaattcacg gactgattta ggtttgcgaa
     9181 ttacggaagt gccagttatt ccagtgacgg aacgtgtcat tgatagtatt gagattgatg
     9241 gacgtgaagg agatttgacc attctgaaag gctggaaaga tgtgactttc ggttttaagg
     9301 tagtgatttg gaaatctgat gtttggacta gctggcagat aattttgcca caaatcctaa
     9361 acgctaaaac tatcgcattt tcaaatgaac caaacgtgtt ttacccaatc aagtatgcta
     9421 aggcgagcgg actaactcag attttatctt ccatgtggga gtttgaaatg gagttaacct
     9481 gttcgccttt tcgttataag accaatgtag cgactatcaa tcgaaccacg tcaggaacgg
     9541 tcaataatcc cggcaacatt tactcattgc cgaagattaa ggtctacggc aatggaacta
     9601 ggacactcac gataaatggc aagcctatcg tgttaaatct gacgaatggc aacctgacac
     9661 tagattcgga tttgaaagaa tgcttttacg gggatgtggc agccaatcaa tacatgacag
     9721 gtgactttcc tgagtttcga gtcggcagta atactgtgac tcttggtaca ggtataacca
     9781 aagtagaaat agaaccgagg tggcgatact tatgattacg ctttacaaca aaacagaatc
     9841 aaatttcaat cataacggtc tcgctgtcct tgatggcaac attctgaacc ccgtggtgag
     9901 tgaggaattg aacggactct tttcctttga attcgattat ccaattcatg ctccacacag
     9961 tgaggaattg ttgccagaaa tgattgtgaa agcaccagta ccagaactgt ccgaccagct
    10021 tttcaggatt gttgaacgag atgatgcact tggtggtttt ttgcacatag tagctcatca
    10081 tattttctat gacctagcca caaactttat tgaagatact tatatcgtca acaagaacgg
    10141 gtcgggagct ttgacacaac tgcttggtgc aactcaattc atgcaccgtt ttaccggtac
    10201 atcaaacatc agtgcggtca ataatgtccg cttggtgcga ctaaatcctg tagaggtgtt
    10261 gcttgatagt gacctagaga atggttttca aagcagttat ggtggtgaaa ttgtacgcga
    10321 taacttcacg atttcaatgt taacgactcg tggcagtaac aacggcgtac aaatccgaga
    10381 caagaagaat ctgacaggat ataaagctga tgttgattac tctggaattg ttactcgcat
    10441 tatgccacaa gggtatgatg gactatttct tcccgaaaaa tacgtggaca gtccaaacat
    10501 caaccaatac gtccagccta aaatcaaagt catcaagtac gacaacgtca aagttggtga
    10561 tgaagaggat gagtttactc aagccgaggc ctatgatgaa cttcgcagat tggctgggtt
    10621 agaatactca gctaacaagg tcgatttgcc aaaagccacc tatgatgtgg agtttgcccc
    10681 gcttgagcga actgaagaat ataaagattt tgcacagctt gaaacgatta atttgggtga
    10741 tactgtttca gttattcatg ccgaagatgg attgaacatc acagctagaa tggtctcata
    10801 caaatacgat ccatttctga aagcgtatat ctcagtaaca ctcggaaaca tcacgccaaa
    10861 attcacggat gtggcgaagg acatcaaccg ggtggacaac aaggcggagc aagccaaaga
    10921 cgatgccaat tacgcattga gttcggcaaa tggtaaaaac accaacttct atggcccgac
    10981 aactccaaat aatccgaaac tcggtgacgt ttggtacaaa gaaaatggtg acaagtttga
    11041 aatgtgggtg tatgaaatcc gtgatggcgt cactcagtgg tatccactta tgacagatat
    11101 gacggcagaa gaagtaaagc aagccgtgga acaagcacaa aaggatgcca atactgccac
    11161 tgaaaaggcc aatcaagcat ttaacgatgc tgtgaatgct ttgaatgcag ctgaaagtgt
    11221 aggtgcaagt gttgaaacgc ttactacatc cgttgaacag aatactagca agatttcatt
    11281 agtggcgagt ggtatgtcaa ctcttgaagg acgagtaatc acagctgaat caactctgac
    11341 agtgcaagct gggcagattg ctagtaaggc aagtcaatcg acggttgata atctgacagg
    11401 gcgtattaca actgcagaaa gcaccatcac ccaaaatgct gatagatttg agctatctct
    11461 ttctaaaaca aataaagcgg tcaatgtttt agccggtatg agcattgcag atttgcagtg
    11521 ggcagttggt ggtctgaata caaatgatgg aaaagaaaat acctatggtg gatacgttcg
    11581 ctcaggtttt acaagagtta gaaatggcga aaagtatcta cttcaaacat ttgtgggtgg
    11641 gaaactttac tctacttacg caacggctta tctgctttat tacaaagagg ataagtcctt
    11701 tttatcgtac acccaatttg gaaacaccac cacaccgctt actgttccaa gcagtgccgc
    11761 ttttattcgc attcgtttaa caactagtga tgcaccagaa accatcagtt gctatatgtt
    11821 gaaaacggat gtgacaaatg gctatgttga tttatccgag ataagtaacg tcgtaaaact
    11881 tacagcgacc actgatgcct tactgttaag tgtttcagaa accaaagaag caatcggcgt
    11941 tccatttaaa gtcaatcaat gggaacaagg aagtttgagt accactgacg gttcagaagt
    12001 gtccgcaacc aattatctcc gaagtgagtt ccttgatgtc aaagtgggag ataagtatat
    12061 cgggcaactg cgtgacggaa ctcaagctgc tttctactat cattactacg gcatggattt
    12121 accttacatt gattttgtcc caacggcaaa acaatttagt gaaaccctaa cggcgggaac
    12181 ttatactaat gcgaccattg cgagttatct agtaagtaag aacgttgaat cgatgagtat
    12241 tacaagtggc gtgacaacaa acccctattc aaattcagga gattatctgg tcatttacaa
    12301 attgccattg gaaggcaaag tcgctccgag tgtaatttcg tggaatggtc gcaaggatac
    12361 atcagataat attgtcttgc tttatacagg tggtgcttgg gaacagattt cgaccgttac
    12421 aagtagcagc aatgccactt attcttggac gttaacggct gagcagatat cagggattac
    12481 tactgacagc gtgtatctag ctttcttttc agttaagact ggcacatatg ctgggattta
    12541 taattcaagc aacacacctt ttactctaaa cccgtcggat ggttcaagtt atcatcaaat
    12601 ctcttatact ggttcgagcg gagcaattac agtcccgagt ggcacattga aaatgcgagt
    12661 ccgagtgaac acgagcatca aacccgatga atatgaagga aatatttatc ctacgaccac
    12721 aaggcaagac tataccaatg ccaacacatt atattcagca attctcatgc agaaagactt
    12781 aatcaattta cgagttggta aaggagacgt gattaatcaa atcaatatct cacctgaaag
    12841 tattttgatt gctggaaaca aggtgcacat cactggtcaa acaaccattg atagtgcagt
    12901 gattagttct gccatgatcc aaaatgcggc catcacaaac gcaaagattg cagatgccac
    12961 aattcaaagt gcaaaaattg caagcattga tgcggcgaag attaccacag gaactttgtc
    13021 ggcagcccgt attggcgcga actcgattac ggctgataag attgctgcta atatcctaac
    13081 agcattgact gcgagttcgt ccattcgaat cacaggaact acgattggat attattcagg
    13141 aagcactctt gtgactgaaa taaactcgca gggaatgacc attcgacgtg gtggaacgac
    13201 agtcggaaca attggtgcaa ataacatcag tggtcactct gattggcgtg gattggtatt
    13261 tgatcttgag tatggaacag agtatatgtc atgggctcat aaagattcct cctctgcgag
    13321 tgtctacacc cagaagcttt cttggtatgc gagaacattg cagtctggta tggagaaggg
    13381 gttccacttt gacgacgaca tttatttacc atcaatgctt cgtataggga cacaacatca
    13441 aatatctgtt ggtgggatta catttagtag ttccctttat actaagattg gctcaagttc
    13501 cggcagcgct ggtattgcat tcaacggttc taatctgatg ctcggagatg atgggaagtg
    13561 ggttgacttt ggagtcatcc gtgaaatctg tgccaaactt gcgggacgaa caatcgcttt
    13621 accaaccggt ttcagtagca acggtacagc gagtggttgg tacaatccgc agtcgtttaa
    13681 ttcaatgacc acatacagtt aggaggccgc catgaacgaa gaaaaagaag aagtgatagt
    13741 aactgaacct atcgaggcag atattccaaa gccaatgaaa gttgggacgg atatggttga
    13801 tttgcttgtg atggtcgtgg atgatctttg tgtccagatt ggtgatttaa aggagaagat
    13861 agataatgct caaaattaga aatgaagatt tggtggacgt gatgagtttc ttaaaaggtt
    13921 ttgacttacc accaaagatt agccgagtgc gaacgaagct tgcaaaactc atccaggtga
    13981 aggttgatga actctatcaa gacgaggtag acttgcttca aaaatacgga aagaaagatg
    14041 agcacggcaa tttggtccaa catgacggtg ccttttcact catcccagaa accgcaactg
    14101 aatatcacaa agaaaaggca gacttgctca acgaagaaac ccacatcaac gtggatgaac
    14161 ttcaagacaa gctgccttta ttggttgctg gatttgaaaa tagtgaagtc accatttcag
    14221 ggaaagatgc tgaaatgctc gatttaattt tagaacaatt ggaggatttg taaatgaaag
    14281 aagtgtggaa ttggattcaa ctcgccatcg caggtggtgg aggagtgatt ggatggtatg
    14341 tgggaggaat tgatggtttg atttatacgc ttgtaacttt tgtggtagtg gactatgtaa
    14401 ctggtctgct tcgtgcaatc aacgaaaaga aactatccag caagattggg gcaaagggca
    14461 tcactaagaa aatcatgatt tttcttttgg ttggtgtggc tcatatgctt gatgtggaag
    14521 tcatcaaagg tggaaatgta ctgcgtgatg cggtcatttt cttctatatc tcaaacgaag
    14581 gaatttcact gctggagaat gccgtggcga ttggactgcc agtgccggaa aagttgcgtg
    14641 aaatcttgca gcagttacac gaaaagaagg aggaaacaga aaatgggaat tagtaatcta
    14701 gcaacaaaat atggatttca gtcatttagc cattactcga atggtcgtag tggtgccaaa
    14761 gtagataaga tagtgattca tcacatggca accaccaact atgatattgt tcctgatatt
    14821 tggaaaacgc gtgaggcgtc agcacactac ggtattggaa aaaatggaga aattcgagca
    14881 tacgtggacg aaggaaacac agcttggcac gctggtaatt ggaacgccaa tatctcaagt
    14941 atcgggattg agcactgcaa tagcactgga tcaccgaact ggggtatcaa tcaagcaact
    15001 attgatgcaa gtgcaaagtt gtgtgcagat attgccaaac gttatggtct agggaaattg
    15061 gtggtgaaca agaatatctt ccctcactca catttttcgg ccacatcatg tccgggacag
    15121 ttgcttggta aactgcagga gattgcggat aaagccaatg cgattaataa tggttcatca
    15181 accccaagca cgccagctag tggtctgtat cgtgttcgca aatcatgggc tgacgccaag
    15241 tcacaaattg gtgcctataa ggacttgaac aatgcgaagc gggctgcaga tagccattct
    15301 ggttataagg tgttcgacca gaacggtaat caagtttatc catctgggtc atcaacatcc
    15361 aacgtgaaaa ctcttgtggt tcaagtgagt ggtctcaacg ttcgcaacaa gccaagttta
    15421 agtggtgcgg ttgttggttc atacaataaa ggcgcaactt ggacgcttcc gaagtcagag
    15481 aagatgacga ttgcggatgg ttgggtatgg gctcgttgcc cgattggata ttgtgctgtc
    15541 ggtaagaata ctggtaagac ggaatctgac gattatattt tgatttcata aatttaatcg
    15601 gaacaaaatt atgtccggat tgttgactta aaatatcgaa cgtgttatta ttaattcaac
    15661 cagaacataa atttgttccg attgaattat agaaagaggt atcgatatgg ttacgtttaa
    15721 agatttgtta ccagaaccaa cagaaagcca acaagctttc attttagatc acgggaaaac
    15781 agaagatgat ggtcagttga aatacgctga tgatgctaaa agttacggtt ggaacatgcg
    15841 ccaatatggt aaattgaaag ccggcgcggt tgttttgaat cgtcatccgg ggaaaattac
    15901 taaagaccgt aagtgggaaa tttacggggg cggatacgtt gagagcattt ctgaacctga
    15961 tgaagctgga aatgttacgg cagtaattac acacgcattt acaatcgaac cggcaattaa
    16021 acaaggtgat tcatttattg aaaacttcga ttggaataca cctagcaaaa agaagcgtaa
    16081 aaagccgaat tcatgggcct acttctggga tcagtatgga atgaatgaaa ttacctatgc
    16141 cgattttgtt gcattgattg aaaatcgtca tcttatccca atcgatgata cacaatcgtt
    16201 gccgtctgaa ctggatctta caaacgagga agttgaagaa attgagcaag aaagttcaaa
    16261 aggatttact gttcttgttg atgaagtggg accaaatcgc acgaatggga ctcagaaacg
    16321 aaaattcact ggacgccata ctgattggga gcgtgtcaac aaggcaaaac aaaaaacggg
    16381 tgctctaggc gaagaaatcg tacttgattt cctcattcag gaagcagaaa agaacaaaac
    16441 aaaacttcct gaacatgttt ctaaaactga aggagatggt catggctatg atatttgtgc
    16501 gtttgaccaa tcaggtaatg aaatccatat tgaagtgaaa gcttcaaaaa caaacttctc
    16561 tgacggattt gaaatgagta ctaatgaggt tgctgcctcc cttggagata caccatataa
    16621 aatttacttt gttcacgacc ttgatgtggc gtcaaaagtt tgcaaaatca aaatttatga
    16681 tggcccaatt actgaagaaa acttcacgat ggttccaacg agctacaaaa ttttcaataa
    16741 gtagtttgac gtctttataa ttcaaaacag cctcacaagt tttattgtgg ggcctttttt
    16801 tcgtctagtt ttctttaagg taggtgttta cagcattttg cattaggatt aaatgatttc
    16861 ttaactgaaa tacctgctct ttgtaattga tgcagaaaag aggcgattct attgattcca
    16921 tacgattatt catcgcgttt atattggctt gtaaaaactg gtataaagct tcttcagcct
    16981 ctatggtttt aaagcacttt tcaaattctt gtttgaagct ttcatcaaat tcctccagtt
    17041 cctttgttgt cgcagatttg caaaaatgtt catattcttt ttctaaccct tccatatcaa
    17101 aactactcat aactttctcc ttcccacaat tgatatttat attgttattt attggatgaa
    17161 gcttgtaaag gcttatttga tgtttgggaa gtaaaaagtc aaaatttaca aagaataatt
    17221 cctactatat ataaacaaac ttagaagcct gcaagagtga ttcgtttcat tcttgcaggc
    17281 ttttttcgtt ttgtgggttc gagtcggtta tttctttcgc atatggatga aaggcaaagg
    17341 aaaatttgtg attgggttta ctttcatgga ttttcttcgc ctgtatcttg aggaggcgaa
    17401 agcaatgact aacaagaaaa cagaaattaa ctattcagat gaagcagcct attacatttc
    17461 acagcagatc cttctgaact tacttgttac cggtaaaatc aacctggaag aatacgtcaa
    17521 aatcgacaat aaaaaccggg aaacattcaa tcctttttta tcaaggataa tggtctgaaa
    17581 cgttgatata acggcacttt agagctaaca tgtcacctaa tggaaaggag ttgagctgat
    17641 gaaaacgata cataaattgg cacgtccaga gttgccggaa gcttccaaac taaaggtggc
    17701 agcatacgct cgtgtatcaa catcaagtaa tgaacagctg gcaagtttac aaacccagat
    17761 tactcactat gaaaatcaca tcaagaacca tgaacaatgg gactatgtcg gtgtttatta
    17821 tgatgaaggg atttctggaa ccaagactgt aaaacgtgat ggtttaaaac gattgattaa
    17881 ggatgcagag cttggaaaga ttgatttgat tctgacaaag tctatttcca gattttcaag
    17941 aaatacagtt gactgcttga atctggttcg gcagctaact gatattggtg tcaccatctt
    18001 ttttgaaaag gaaaacatta atactggtga tatggaaagc gagttgttgc tttcaatttt
    18061 atcaagcttg gcagaaagtg aatcctattc ccattcagaa aacttgaagt gggcgattcg
    18121 aaaccggatg tctaaaggga aattcaaagc ttacccgccg tatggctaca agcggaaagg
    18181 ctatgatttt cacatcgttg cagctgaagc aaaagttgtt aaacaaatgt tccagtgggc
    18241 attagatggc atttcgactg gaatgattgc caagcggtta aacgatggaa aacaattcac
    18301 taaacgtggg gcaaattggc aagcaacatc agtcagaagt attttgaaaa atgaagttta
    18361 taccgggact ttgacgatgc aacaaacctt tgttgatgac cagtacaaac gacgcataaa
    18421 caagggcgaa ttgccaatgt accagatcaa aaacaatcac gaacccatag tatctaaaga
    18481 agattacgac cgagttcaag aactcattcg gagaaaagct gaggccaaag ggaatactgg
    18541 tgacgggaaa aagtacacca agcgatacgc attttcaggc aacatcatct gtgataaatg
    18601 cggcagcaaa ttcaagcggg ttcacactta ttggaaaggt aatacgaaga aggttcagtg
    18661 gaagtgtacg gggcatctta aaaataaaga tagctgcgat gcattaccaa ttacggatga
    18721 agatttgaag gttgcttatt tgaccatgtt gaataagttg attttcggac acaagttggt
    18781 tctggaacct tttatgaagt ctttgaataa agcaggtgcg aatcaggaag ggctcgaagt
    18841 gcttgctgat gaaatcgaca agattcaaga aaagcttgag gttctggcaa gtttgactgc
    18901 ttccagtatt ttatcaacga aggtgtcttt agaagaacaa agcaaacttc aacaagagag
    18961 ggctgtcgca caagaaaagc agcgcctcat cattgagtca gtcaacggca aaagcactca
    19021 gcaaattgaa ctagaagcct tatacaaatt cgcgcggatg gcagagatgt tgacggactg
    19081 ggatgaagat ttattcaaga agttcacaaa cagcattaca gtttacaacc gccaagaagt
    19141 cggatttgaa cttaagtgcg gactactttt gaaagagagg ttaccagaat gaacaataca
    19201 attccatacg ggtatgtcat taagaatgga gaaatcaacg tggatgaaca gcaagctcaa
    19261 caaatacgtg agttcttcaa tatttacctt gagggtgttg gcttacaaga agctggcaaa
    19321 cgctctggca ttaacaagcc gcatcccttt atgaaacgta ttttgatgaa tgaggtttac
    19381 ttaggaaatg agatatatcc aaaaatcatt gatgaggata ttttcttcca agcggatctg
    19441 gagcaagaac gaagaattaa gttctttaaa aaacgcaagg agatcaaagt gccagatacg
    19501 aaggtaatac ccactcattt taaatctggc aagattaccg aaaagtattc tgacccattc
    19561 aaacaagcgc aatatgcata ttcaaaaatc aaggaggtta agtgaaatgg caggaaaaca
    19621 ggtgacggtt attccaatga agcctagaca atgggcagca gagaataccg aagagaaacc
    19681 aaagttgaaa gtggcagcct attgtcgtgt ttctactgaa atggaggaac aggcgagttc
    19741 ttacgaagca caggttcaac actatacgga ctacattcaa agaaatcccg attgggagct
    19801 tgtaggaata tttgcggatg aagggatttc aggaactggg acgaaaaagc gtgacggttt
    19861 taaccggatg attgaggcgt gtcaaaacgg cgaagttgag tacatcatca caaaatcaat
    19921 ttcacgcttt gcccgtaata cggttgattg cttgcaatac atacgacagt taaaagaact
    19981 gaagattgca gtgttttttg aaaaggaaaa catcaataca atggatgcga agggtgaggt
    20041 acttttgacc attatggcaa gtctggccca gcaagaaagc cagtcactaa gtcaaaacac
    20101 caagatgggc gtacagtatc gtttccagca gggacagcta cgtatcaacc acaatcattt
    20161 tcttgggtac actaaggacg aagatggtaa cttggtgatt gaaccaaagg aagcagaagt
    20221 catcaaacgc atcttccgcg aataccttga gggcagttcc ttgcaagaaa ttgctaatgg
    20281 tctaatggct gatggcatcc taacgggagg gaagcgaaag ctctggcgag gagaaggagt
    20341 cagattgatt cttcgaaacg agaagtacat gggggatgcg ctgcttcaaa aaacatatac
    20401 taccgatttc ttgacaaaga aacgagtgaa gaatgacggg agttacgctc agcagtatta
    20461 tgttgaagat agccatccgg ccattatccc aaaagatatc ttcatgcaag cccagcaaga
    20521 actagaccgg cgtaaatcaa tgaaaaacaa aaagtcacag tgcttttctg ggaagtatgc
    20581 gttatctggt atcacagttt gcggtgactg tggcaatgct taccgcagag ttcattggaa
    20641 gaatcgagga actgtgtggc gatgcaaatc cagagtggat aaacgagaac ataattgcac
    20701 gggtcgaaca atctacgaaa cagacctgca tggagctgtt atcaaggcaa tcaatgaaac
    20761 gttaatcgat cgtgatgctt ttcttcagca attaaatgac aatatcaact cagttatgac
    20821 tgatggatta actgaccgat tggcagaact agacagtcag ttgaaagagc ttgaagctga
    20881 aattattagc ctggcagttg gcggtcaggg ttacgatgaa ttggcaagcc aaatattttc
    20941 actaagagat gaacgagatg cggttgcaaa acaaatagca gcgaatacta atttgcaaca
    21001 acgggttgat gagatggtag tctttgtaaa agaacacgat gtcatcaatg agtataatga
    21061 agtgcttgtt cgaaggttga ttgagaaggt caccattttt gaaaagaaca ttgttgttga
    21121 tttcaaaagt ggggtgagag tgactgtaga aatttaattt gaatagatag gactagacgt
    21181 taatgtgggt agccatatta acgtctttat tgattggatt tcataaagtg attgatgata
    21241 taatatatta tgtaaaaaat aggctggggg cgaagttctg tgagccaatg gaataccatt
    21301 gatgaattaa gggatatttt tattcgtaaa tatacggatt ctgagttgag taatgaactt
    21361 gaaaaggcct gcagcgaaga atatgaattg atgcgtgatt ataatgggcg tcagatttta
    21421 gagttattac agaatgtaga cgatgcctat ggagatataa aatcagaaaa tgatgctagc
    21481 aatgacgaag tgaaagttca gataacatat aaaaataata tcttggaagt tgggaataca
    21541 ggcacttcat tttcaaaaga tacaattgaa agattgtgct taggaagagc gagtaataaa
    21601 tcatcacaaa atatcggaaa taaggggact ggttttcgtt ccttgctaaa cgatgctgag
    21661 tgggtcgaac tgtattcggg agaatttgct attcgattta gtgaagaatt cacaaaaaag
    21721 ctttttgagc agtatgtatc aagagattct gacaaattca gtgaattaat ttttgagcag
    21781 aagaaaaatt ggaaaaaaga agattatgat ttatgttttc caataatgaa ctgtcctcaa
    21841 ccaataaaca aaattgattc tggttttgat acattaattc gagtaaaact aaaagaaact
    21901 aatttaagta aaggcacgag tgtttctaaa caacttcagc agccatttta taagtcactg
    21961 ttatttttac caaatataac aaaaattgtt attgaaacaa ctgatgatga aaaaatatca
    22021 gagaaaattg tggatggaaa tgatgtattg atagaaaaaa gtgatgttac tggtcaatca
    22081 ccgtctgaag aatattttgt ttttgacaaa gaatctatga taggagataa agttgctaaa
    22141 attgcactag ccgtttctaa agattctgat tacgattact ccaaagaaaa actatattgc
    22201 tactttccag tacgtaattt tggtacacca attcatgcac ttataaatgc accgtttata
    22261 acaaataatt cacgtgatga cgtcccggat gatagtgaac aaataaataa gaaaatattt
    22321 attgaggtgc tcaaatttat ttctgaagta gcagaaaaat tatctaagcc tgaatatgaa
    22381 gacatagcat taaaaatggt aactcctttt aaagagaata aactatggga cttagatgta
    22441 tttgacttaa tgaacgaatt cttgggtatt ttatctaatg cccaaatact accgactgtc
    22501 aattggcaat atatttctat caaagataat ccaaaacttt tccttcaaga attccctgag
    22561 gagctaatag gtgagacatt ttccaccttg ttgcaattat tagaaacaaa cgaaaatgac
    22621 caactagtac aagaacttgc taattataat gaatacactg gcctgtcgtt tgttgtagat
    22681 gaactatctg ataaaatcaa cattctttca tcttcttggg aaaatagaat aaggacaaaa
    22741 gtgttcttat ggtggagtga gcaatataaa agttctccga ttgtccctaa gctattaaag
    22801 gacaatagtg acaattggtt gatgaaatca gataaggtat tcttaccgac cgatacaggt
    22861 gttactgtgc ttccaaaaga gctgtcttct tgggttagtt taagcatttt gaaacaaaca
    22921 tatgttgatg agttaattta tcagattaag acttcatcaa gttggaaaaa taaatgggaa
    22981 gaaacagcta atgcatacaa agctgaacgt acaggagata aacgtattct agatgcattc
    23041 agtgaaaaat atcttgcaat tgaattcact gagcaaagca gttcagattt gatcattgga
    23101 acgattaatc gacaaattga cactgttgaa aaatctatca gtttcattaa ttggttttat
    23161 gataactaca atgaaaaact atcagctgga agtgagctgt ctaaagttcc atttaatctt
    23221 ccaaacgttg ctggtgacgt tcaaccttcc aataaaatgt atattggaaa agagtatggc
    23281 aatgaattag gagataagct ttttcaagat acagattaca cggcattgaa aaaaatagat
    23341 tgctctagtt ttgctgagga gagagatttt ctgtcattta ttcttcgatg tggtgtttcc
    23401 aaatatccta agatttatga taacgattct ttaaaaaatg atgttggat
//
LOCUS       DAYYFD010000043        23271 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000043 DAYYFD010000000
VERSION     DAYYFD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.043"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1653
                     /locus_tag="ACHA25_001582"
     CDS             <1..1653
                     /locus_tag="ACHA25_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8762876.1"
                     /translation="KFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFNLVV
                     PQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTYKIK
                     LREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKSELG
                     IKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNGPFT
                     LEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDVILS
                     GELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVKQVL
                     GDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDSLEF
                     DLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGGWAA
                     DYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIKIIS
                     DDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1723..2085)
                     /locus_tag="ACHA25_001583"
     CDS             complement(1723..2085)
                     /locus_tag="ACHA25_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8762877.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2153..3094
                     /gene="opp3b"
                     /locus_tag="ACHA25_001584"
     CDS             2153..3094
                     /gene="opp3b"
                     /locus_tag="ACHA25_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8762878.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3095..4138
                     /locus_tag="ACHA25_001585"
     CDS             3095..4138
                     /locus_tag="ACHA25_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8762879.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4144..5214
                     /locus_tag="ACHA25_001586"
     CDS             4144..5214
                     /locus_tag="ACHA25_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762880.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5204..6157
                     /locus_tag="ACHA25_001587"
     CDS             5204..6157
                     /locus_tag="ACHA25_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762881.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6169..7179
                     /locus_tag="ACHA25_001588"
     CDS             6169..7179
                     /locus_tag="ACHA25_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8762882.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7464..8285)
                     /locus_tag="ACHA25_001589"
     CDS             complement(7464..8285)
                     /locus_tag="ACHA25_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8762883.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8382..8660)
                     /locus_tag="ACHA25_001590"
     CDS             complement(8382..8660)
                     /locus_tag="ACHA25_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762884.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8928..10937
                     /gene="metG"
                     /locus_tag="ACHA25_001591"
     CDS             8928..10937
                     /gene="metG"
                     /locus_tag="ACHA25_001591"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8762885.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11416..12003)
                     /locus_tag="ACHA25_001592"
     CDS             complement(11416..12003)
                     /locus_tag="ACHA25_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762886.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12178..13500
                     /locus_tag="ACHA25_001593"
     CDS             12178..13500
                     /locus_tag="ACHA25_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8762887.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13655..14431
                     /locus_tag="ACHA25_001594"
     CDS             13655..14431
                     /locus_tag="ACHA25_001594"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8762888.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14424..14993
                     /gene="rnmV"
                     /locus_tag="ACHA25_001595"
     CDS             14424..14993
                     /gene="rnmV"
                     /locus_tag="ACHA25_001595"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8762889.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            14986..15870
                     /gene="rsmA"
                     /locus_tag="ACHA25_001596"
     CDS             14986..15870
                     /gene="rsmA"
                     /locus_tag="ACHA25_001596"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8762890.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16321..16950
                     /locus_tag="ACHA25_001597"
     CDS             16321..16950
                     /locus_tag="ACHA25_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762891.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17074..18183)
                     /locus_tag="ACHA25_001598"
     CDS             complement(17074..18183)
                     /locus_tag="ACHA25_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762892.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18392..18814
                     /gene="mgsA"
                     /locus_tag="ACHA25_001599"
     CDS             18392..18814
                     /gene="mgsA"
                     /locus_tag="ACHA25_001599"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8762893.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19001..19099
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19137..19673
                     /locus_tag="ACHA25_001600"
     CDS             19137..19673
                     /locus_tag="ACHA25_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8762894.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19713..21476)
                     /locus_tag="ACHA25_001601"
     CDS             complement(19713..21476)
                     /locus_tag="ACHA25_001601"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762895.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21479..23194)
                     /locus_tag="ACHA25_001602"
     CDS             complement(21479..23194)
                     /locus_tag="ACHA25_001602"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8762896.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7473 a         4278 c         4746 g         6774 t
ORIGIN      
        1 aaatttacat ttggtttggt tgcggtatgc ggccttgtac ttgctggatg ttatggcggc
       61 agttcagcca caagcagtag ttcagcaaaa agcggttcag cagatggagg aggcgtattt
      121 aacttagtcg tgcctcaaga aatgcctaca gctgacttat cagttgcaac agatacaatc
      181 agttttacag cattgaataa cgtttatgaa ggaatttacc gtcttgatga agacagcaaa
      241 ccacagccgg caggagctag cgagttggca gaagtcagtg aagacggatt aacttacaaa
      301 atcaaattac gagaagacgc aaaatggtca aatggtgatc ctgttacagc agcggattac
      361 gtttatggct ggcaacgaac agtagaccca gctacagctt ctgaatatgc ttacctgtat
      421 gcgccagttg aaaatgcaga agacattaca gctggaaaga aagataaatc agaactaggg
      481 atcaaagcag ttggtgacta tgaattagaa atcaaattga cgaaacaaac accatatttc
      541 caatatctgc ttgctttccc atcatttttc ccacaaaaac aatccgtagt ggaagaaaat
      601 ggggatgcct atgcatctgc aagtgataaa gctgtttata atggaccatt cacattagaa
      661 gattttgatg gaccaggaac agatacagaa tggacttata agaaaaatga tcattactgg
      721 gacaaagata cagtaaaact ttctgaaatc aaagtcagtg tagtaaaaga atcttctact
      781 gcattgaact tattcaaaga tggccaagca gatgacgtga tcttgtctgg tgagttagca
      841 cagcaaaacg caaacgatcc agcttatact tctgttaaag aagcgcggac aagctatatc
      901 gaattcaatc aacgtgaaaa gaattcacca ttcaacaatg tgaacctaag aaaagctatt
      961 tcttattcta tcgatcgtga agctttagta aaacaagtac taggtgacgg atcagttgtc
     1021 tctacaggat tgatcccatc agatatggta aaaagtccaa caacgaacga agactttgca
     1081 aaagaagctg gaaaattagt ttcttatgac aaagataaag caaaagaata ttgggaaaaa
     1141 gcgaaaaaag aattgggcgt tgattcttta gaatttgatt tgatggcttc tgatgatgat
     1201 tcatcgaaaa aagtaatcga atatgttcaa aactcaatcc aagaaaactt ggatggcgta
     1261 acagtcaaac caacacctgt tccattctct gtacgtcttg accgctcaac ttcgggtgat
     1321 tttgacgcag tactaggcgg atgggctgct gactatgcag acccaagcag ctttactgat
     1381 ttgtttgtca caggtaattc atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa
     1441 gcagttaaag cagcttcatc aacagacgct ggcgatgaac aagcccgttg ggatgatttc
     1501 caaaaagcaa tcaagatcat ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca
     1561 gaaggtcacc tagtgaatga aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca
     1621 tgggattaca aatggacata cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa
     1681 acgaggctgc ggcaaatgtc gcaacctcgt tctcatattc acttatccta acaaagaaaa
     1741 aagcatgaaa atagcagaaa gagcaattag tattccggca atgatcagcc aaaagctgta
     1801 cataccgtag ctggcagacg agagcgtgga aaattctggt tttttctttc ggttccttgt
     1861 tcgtgcaaga tggaccgatc gctgaaaatg atcgaagaaa cccgaagaaa agacccataa
     1921 aaaaccgccg ataatcagaa acggcaatga ccataagaat agatcattag ataattgaat
     1981 gagtgtaaag gaatggttga tgagattttt tattaaaata accagaatca gtagaaaagc
     2041 tactgcgtag gggatagatt ttttttcatt tgattgctcc tttatttttg gttcattatc
     2101 taataatgta gcgaaacatc tgtcagaagt caaaagctag ggggaaacat tagtgaatag
     2161 ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc atcacgcttt ggttaattgc
     2221 aacaatcacc tttttcttga tgcaattgct gccgggcacg ccatatacga atcaggaacg
     2281 tttgagtcct gaaacaatcg agatgctaaa taaacaagtc ggtttggata aaccagttat
     2341 cgtacaatat gggatttatc tttctaattt gcttcaagga gattttggaa tttccttcca
     2401 atttaaaaac cagccagtcg cacatttgtt agctggaaga atcggaccat ccttgcagtt
     2461 agggttacag gcaattatct ttggaacagt cctagggacg attttaggaa cgatctctgc
     2521 catgaagcaa aatacatggg cagatacatc ttctacactc gtagcgatat taggtcgttc
     2581 cattccaaac tttgtatttg ctgttttatt acaatatatc tttgcaatca aattacaagt
     2641 tttgccaatc gcaaaatggg atggctttgt gtacacaatc ttaccaacaa ttgcgttggc
     2701 catgtcgccg cttgctgact ctgctcggtt tatccgtacg gaaatggtgg aagtactgca
     2761 tagtgattat gtggaattag caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa
     2821 acatggtctt cgaaatagtt tgattccatt gatgacttta ttaggaccgt tagctgtagc
     2881 attgatgacc ggatcactgg tggtagaaaa tatctttgct attcctggta tcggggaaca
     2941 attcgttaag tcaatcacaa caaatgatta tcctacgatc atggctgtaa cgatccttta
     3001 ctcatttatg ctgatttttg ttattttgat cgtggatcta ttgtacggtc tagttgatcc
     3061 aagaattcgt gtttcagaag ggagccgagg ataaatggaa atgataccaa agcgttatga
     3121 aacagttgca gatatccctg cggatgaatt tcttccatta caaaaaaata ccgaagaaga
     3181 acgtgaacag attactgcac cttctttgaa ttttatccaa gattcatggc gccgtttaaa
     3241 gaaaaacaaa gctgccgtta tttcaatggt tttactattg gtcatcattt tgatctctgt
     3301 gatcacaatc tttgtttctc ctcatgatcc aactgcccaa aacgtggctt atatcaattt
     3361 gcctcctcgt attccgggaa tcaatatcga tggattgaat ggaaaagcaa tggttggtgg
     3421 tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg
     3481 aacagacggt ttgggacgtg atgtattaag ccgcttattt atgggaatga gaatctcgtt
     3541 gctgatcgca tttattgctg cattactaga tgtaacgatt ggtgtagctt acgggttggt
     3601 ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc
     3661 aggaattcca aacctcgttg tcatgatttt gatgttggtc gtttttgaac caggaatttt
     3721 ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca atggcacgta tcgttcgtgc
     3781 acaaacaatg aaattaaaag atcaagaata tgttttagct ggattgacat taggtgaatc
     3841 aagaatgaaa attgcattca aacatatttt gccgaatatc tctagtgtca ttattattca
     3901 aatgatgttt agtattccat ctgctatttt cttcgaagcc tttttaagct ttatcggttt
     3961 aggtcttacg cctccgtcag cttcattagg aacgatgttg agcgaagggt acaagacatt
     4021 cctgtatctg ccttatctat tatggattcc ggcagtaact ttatcggtta tcatgattgg
     4081 ctttaactta cttgctgatg gattgcgtga tgcctttgat cctaaaatga aagagtgaag
     4141 acgatgacga aaattttaga agtgaaagac ttagaaatct cttttgatac gtatgctgga
     4201 aaggtacgag cgattcgcgg tgtcaatttt catttgaata aaggagaaac attggcgatc
     4261 gttggggaat ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca
     4321 agcaacgcca atatagatgc tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa
     4381 tcagaaagag agatgcaaaa aatccgtggt aaagaaatag cgatgatttt ccaagatcca
     4441 atgacctctt tagatccgac gatgcccatt gggaagcaag tcgctgagtc ccttagaaag
     4501 cataataaag tttcaaaaaa agagggacaa aaagctgcat tagaattgct taaattagtt
     4561 ggaatacccg aagctgagaa acgtatcaac agttatccgc atcagttctc aggaggtcag
     4621 cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc cggaaatcct gattgctgat
     4681 gaacctacaa cagcgcttga tgtgactatt caagcacaga ttttggagct gctgaaagaa
     4741 attcagacaa aaatcgatac atcaattatc tttatcactc atgaccttgg tgtagtagcg
     4801 aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg
     4861 gaagaaattt tctacaaccc gcaacatccc tatacttggg gactgcttgg ttcaatgccg
     4921 acattagata gtgcgaacga tcgattgtat gctattcctg gttctccacc ggatttattg
     4981 aacccgccaa aaggagacgc attctatcca agaaatgaat tcgcgatgaa aatcgatgca
     5041 gaacaagaac caccattttt tgaactatca aaaacacaca aggcagcaac ttggctactt
     5101 gctccgcagg cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt
     5161 aaagagaaac aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa ataaactact
     5221 tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt
     5281 aaatgacatc agctttcaca tttatgaagg agaaacattc ggtttagttg gagaatctgg
     5341 aagcggaaaa tccactactg gccgtacgat cattcgcttg aatgaaccta ctgatggtga
     5401 gatattattc gacggtcaag atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt
     5461 ccggcatgat gttcagatga tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa
     5521 agtcagagat atcattgccg aaggaattga tgttaatgga ctagctaaat catctgaaga
     5581 gcgtgcaaaa aaagtcgatg atctattgag aactgttgga ttgaatccaa gccacggtac
     5641 tcgttatcct catgaatttt caggtgggca acgccagcgt attggtattg cacgagcatt
     5701 ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt tctgcactag atgtgtcgat
     5761 ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat tgacctatct
     5821 atttattgca catgatttgt cgatggttaa acatattagt gaccgaatcg gtgtaatgca
     5881 taacggcttg ttattagaga tgggaacaag tgatgaaatc tataatcacg gtgttcatcc
     5941 ttatacagaa agtttgttat ctgctattcc tctaccagat ccagaccatg agagaaaacg
     6001 cagaagaatc aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat
     6061 cgcaccagaa cattttgtct atgcaactga gcaggaagtc ccatattacg caaaaaaact
     6121 aaaacgacaa aaggaatctc ttctggttgc taactaggag ggacatagat gctaaaagct
     6181 tttaaattcc gtatgtatcc gacagaagaa caaaaacagc aattgattcg tacttttgga
     6241 tgtgctcgat ttacctataa ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca
     6301 ggagtagcca atttttcgct cacacccgct acattaaaaa aagaataccc attcttaaaa
     6361 gaagtagaca gtcttgcttt agcaaatgca cagcttaatt tggatcgggc ttttcgcaat
     6421 tatttcaagg gacgtgccag cttcccaaag cttaaaacga aaaaaagtat gtggcaatct
     6481 tatacgacca acaaccaaac ccgtacgatc tatctggaaa atggctactt aaagttacca
     6541 aaacaaaaag aactaatcaa gataaatcag catcggccag ttgaaggatc gattcgctcc
     6601 gctacgattt ctgctcgata taatgaagaa ttttatgtcg cactactttg tgatgtttcg
     6661 ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg cctatcatcc gaagacattg
     6721 atccaaacat ctcgacctct agaagtaact ttaccaaaat ttcaccagac agaagaaaag
     6781 ctgcaacatg cacagcggaa attgaatgtg aaagtccgct ccgctcacca tagaaagatc
     6841 aggttagatc aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga tctttatctg
     6901 aaacaaaaga accagcgaga agattatctt gaacaattat ctgggaaatt ggtcaaacaa
     6961 tatgactact tatttgtaga atcttttcca aaagaagagg cacatgctga tttttctatt
     7021 cacgactggc ataaattaat aacaaaattg cgttataagt cccaatggta taataaaaaa
     7081 tttcttttga tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta
     7141 gtagaaaaaa tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg
     7201 agcgttaggg ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt
     7261 cttttgaaga aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg
     7321 tacgagcttt ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat
     7381 tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag
     7441 gtctgaatct tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt
     7501 gaaggacggc cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct
     7561 ttcttaaaat tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact
     7621 tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt
     7681 tccattttat tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc
     7741 aaagtatctt tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg
     7801 ctagataaat ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca
     7861 tcatctccgg cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc
     7921 cagcctcgcg gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt
     7981 gaaataacca caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca
     8041 tccttttgga caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt
     8101 tggatcaaca aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg
     8161 acagtcaaag agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat
     8221 tcttcttcac ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg
     8281 ttcatctgac cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg
     8341 aaaaaaaacc aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat
     8401 ggaagtatga gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct
     8461 tcacgaatgg caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc
     8521 caagctgctg tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga
     8581 atataaaaat agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa
     8641 aaaagatgtt tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta
     8701 taccacgtgt gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa
     8761 acccgcattt attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc
     8821 gtttatccag tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac
     8881 tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg
     8941 aaacttttta tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact
     9001 cgtatactac gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg
     9061 tgttttatct cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac
     9121 taggtgtaaa accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga
     9181 aaacgcttga tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag
     9241 ctgttcaaaa aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg
     9301 aaggctggta ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt
     9361 acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat
     9421 tggtaaaaga agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat
     9481 actatgaaga acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca
     9541 actttatcaa accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag
     9601 taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt
     9661 atattactgc actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg
     9721 cagatgttca gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca
     9781 tgctgatggc ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga
     9841 tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg
     9901 aacgttatgg tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag
     9961 atggtgtctt tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc
    10021 tagggaactt attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc
    10081 ctgactatgc ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg
    10141 ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa
    10201 tctgggtgct gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag
    10261 caaaagacga agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt
    10321 tgcgtatcgc agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca
    10381 atcaactagg attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc
    10441 cttcaggaac aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag
    10501 aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag
    10561 tcaaatggga tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg
    10621 atgtatttga aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag
    10681 gggcagataa gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct
    10741 tgtcaggaat cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca
    10801 ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt
    10861 ctgcagaagc accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg
    10921 gctcagaaat cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt
    10981 ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat
    11041 aatacagaaa cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg
    11101 atttttgtta atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg
    11161 ataaagggtc gggcagattt taattgattc atctggtact cttttttatt gtccttacat
    11221 tcataaagtt tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg
    11281 atgtgttaat tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg
    11341 attgaaaata tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat
    11401 tttgtaaaaa tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct
    11461 tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg
    11521 tccgatacca taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa
    11581 aagcaaggag ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat
    11641 cactccaaaa ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt
    11701 aaatgttccg atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa
    11761 aagcccaacc aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa
    11821 tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt
    11881 cattaatcca aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg
    11941 ggaaaaatcg acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc
    12001 cataaactgt tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa
    12061 tacatccgtc ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa
    12121 aatattctca gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg
    12181 aaaggcaaac taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc
    12241 gtgatcggtg caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca
    12301 agtttgacat tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca
    12361 gtggctgaat tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct
    12421 gcgtatggaa aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct
    12481 gctaatattg ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta
    12541 tctaccgatt atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat
    12601 ttgttaggga caaaagtcgg tgcttctgta caatcagtaa cgttgattgt taaattaata
    12661 ccaattgctg tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta
    12721 tttccaatcg aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta
    12781 gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa
    12841 cggccggaaa aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt
    12901 tatctattga tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat
    12961 ttgaatgcag cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc
    13021 actattggga tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc
    13081 cgcgtgcctt atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag
    13141 ctttcaaaga agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt
    13201 attatgatga gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg
    13261 ctattcagtt tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg
    13321 ccacgtccat ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct
    13381 attttcattt taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt
    13441 gttaccttga taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa
    13501 aatatttcaa caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt
    13561 ctatttcaat aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta
    13621 tgtgctaaag taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt
    13681 aaatgctgaa cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg
    13741 ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt
    13801 aagccatgac tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag
    13861 ttatacgaaa gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc
    13921 attaggggaa attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa
    13981 agtatttcgc agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac
    14041 tagagaagca cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg
    14101 tggaatcatg catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg
    14161 gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc
    14221 tgcagcaaat gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc
    14281 agttcctttt agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat
    14341 tgctgaactt cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca
    14401 ccgattattt ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg
    14461 aaggaaaaga cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa
    14521 tagggtctgc aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg
    14581 gagtgatcgt ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg
    14641 aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa
    14701 agggaagcct tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg
    14761 tcgtgacgcc agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag
    14821 actatggttt aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt
    14881 tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta
    14941 cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt
    15001 gaaattgcga caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa
    15061 aaaagtttag gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact
    15121 gcgcaaatag atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg
    15181 gaacaattag ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc
    15241 cctgtattgg ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt
    15301 ttaaagaccg atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa
    15361 gtcgtagcga atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca
    15421 gatttggttg tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct
    15481 gctgaaccag gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa
    15541 gccagtttag cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca
    15601 gcgattttga aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca
    15661 tttttccgat tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg
    15721 cagcattcgt atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca
    15781 gctggaatcg atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta
    15841 agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa
    15901 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt
    15961 ttgtttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg
    16021 ggcctgccgt ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt
    16081 tgagttagtt ggtcttatta cttgatgcaa gcttttgacc actattatgc cgaacaagga
    16141 gatgtgacac aaattttgtc acatctccta tttttgtttc ttgatcaaaa aattcttata
    16201 cttgatactc acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt
    16261 agtaagtgag ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa
    16321 atgagaaaaa attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc
    16381 attatcccaa tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct
    16441 catgtgccat tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta
    16501 ggtacgacat ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca
    16561 catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta
    16621 aaaaatggac agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt
    16681 gtgattcatt ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg
    16741 cctgaaacat attattctca aggatatttt tatacagtat tcatcttgat ggggattggt
    16801 gggcttatcc acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc
    16861 aaatattttg atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt
    16921 caagggtacg cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc
    16981 tagtaatgga taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca
    17041 acatcttctg gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt
    17101 ttctttatca aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg
    17161 tttatggaaa tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta
    17221 aagcatcgtt tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc
    17281 aggtgaagct tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat
    17341 aacttctttt ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg
    17401 aagcttaagc ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt
    17461 catggcagga gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc
    17521 tttcggtgta ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat
    17581 agtcattgct tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg
    17641 aagtttggca atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg
    17701 ctcatccatt aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg
    17761 gcgttgtcca ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat
    17821 ctctgctgca ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa
    17881 gccaaaagcc atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac
    17941 catggcaata tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag
    18001 ttctccttca gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca
    18061 accggaagga ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt
    18121 tacagaataa ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac
    18181 cataatacgt tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag
    18241 cgttaactaa cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct
    18301 agttttaagc tttaataaat atccataaga aataaaaact gatacactgg agtaaataag
    18361 tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg
    18421 aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga
    18481 actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca
    18541 tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga
    18601 tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga
    18661 tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg
    18721 aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt
    18781 ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt
    18841 ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata
    18901 cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa
    18961 aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc
    19021 gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt
    19081 tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga
    19141 agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg
    19201 tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta
    19261 ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt
    19321 tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat
    19381 tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt
    19441 ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac
    19501 cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc
    19561 gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac
    19621 gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa taaaaaacaa
    19681 gcagtcttcc catcccagaa gactgcttgt ttttattgtg ctgtgaattg tgcttgatag
    19741 agccgtcggt aataaccatc tttttgaagc agctcatcat gggtgcccat ttcgacaata
    19801 gctccctgat ccatcactaa tatctggtcc gcatttttga ttgtggacag gcgatgggca
    19861 atcacaaaac tggtttttcc ttccatcatt gctaagaagg cttgttggat ttttttctct
    19921 gtcaatgtat cgactgagct ggtagcttca tctaagatca acataggggg attactgatc
    19981 atcgttcgag caatcgtcaa cagttggcgt tgtccatctg atatctttaa cccttgaccg
    20041 ccaatcttag tatctaattt ttccggcaaa cgttctacaa attcatacat ataagaggct
    20101 tttagggcat cattgatttc tgcgtcactt gcttccggat tcccaaaacg aagattttct
    20161 cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca ttccaaaact tttacgtaat
    20221 gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg taattgttcc ttttgttgtg
    20281 tcataaaaac gcatcagcaa attaacaagt gtggattttc cggcccctgt ttttcctact
    20341 atagcaatgg tttgacctgg ataagcagta aaattgaaat cttcgatcaa acgctgcttg
    20401 gggtcataag aaaaagaaac atgatcaaaa gagacttttc ctttgatatt tttgggttcc
    20461 aaggcatagg catctggcaa atcaggcttt tcttctggct gatcgatcaa atcaaatgct
    20521 cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat
    20581 ggtttagaaa actgacttga gtagatcgta aagctggaaa tcacaccaac agtgattgac
    20641 gaaccactct tcaatgccaa taggccgcca actaaaccaa tcgataaata agcaaggtga
    20701 tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt
    20761 tcatatagat cagcatttag cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa
    20821 gctttcacga ttttctggtt accgattcgt tcggtcacaa agcccgagat atccccaata
    20881 atcctttgct gtctatcgaa atttgtttgt gaagcacgag caacgatcca actcacaagg
    20941 aaaataattg gtgtagaaca aagaacgacc aatgtcagaa gtggacttag acgcatcata
    21001 aaaataaacg aaagaatgat gattgccact ccagaaaata attgattgaa ggccgcagaa
    21061 attgcaatgg aaatattgtc catatcattt gtaaatcgac tgacgatatt tccgtgggag
    21121 ttttgatcat aataacgaag tggcaatcga ttcagatgag caaatgcatc ttttcttaat
    21181 tttgcaacgg ataagtaagc taaccgattg cttaacactt ggatgagcca ttgactgact
    21241 acgttgatta aaacgatacc accgaataga ataagtaaat ggaacagtcg attgaagcgg
    21301 acctgtcctt ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt catcacaact
    21361 gttgcaatcc cattgatgat tcctagtatc acagctagcc aaatgtcttt tgcataggca
    21421 gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata atttttttac tttcataact
    21481 attcttcctc ctgtgatgcg acgatttcct gatattctgc tgattgagca agtaattctt
    21541 catgggtacc ctgagcagca atcttacctt gatccatcac taagatatga tgtgcttctt
    21601 gaatagagcg gacacgttgg gagataataa tcactgtact gccaagataa gtctgtagcg
    21661 ccttacgcaa atccaagtct gtttgataat ccaacgcact taaagagtca tctaaaatca
    21721 gtaatgctgg cttggcaatc aaagctcttg caatcgtcag tcgctgtttt tgtccgccag
    21781 agaaattttt cccattttcc atgacttttg tatctaaacc atttggaagc tgacggacaa
    21841 attcttctgc ctgagcgatt ttcagagctt cccaacattc tttttctgtg gcgtctttct
    21901 taccccattg aaggttttca cgaatagtcc cggtaaatag tacagaagtt tggggaacaa
    21961 gagcaatttg ctgacggaga tgatccagct gccaagaacg aacatccatt ccgtccatta
    22021 taactgtacc ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc
    22081 cgcttccggt tggaccaatg attccaagga ctgtatttcg cttcagctga aatgtaatat
    22141 tttggagaga aagaccagaa tcaggagtat aacggaagtc aacatggtca aaagcgataa
    22201 ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc gaattttctg
    22261 tgtctagcac ctctttcacc cgatttcctg aagcttcggc acgtgtgaag atcacgacga
    22321 ggtttgaaac aacgatcagt gccaaaagca tttgattcat ataattgatc aaagcaagaa
    22381 cttgtccttg ttgcaagcta ccaatattta ctttgatccc gccaactgtc agcaaacaga
    22441 tgatcccgac attcatgatc aaggttgtag caggtgataa tagggcagag atattggaaa
    22501 cacgtaaata attgttcgct agatcatttg tgcttttatc gaacttctct gtttcttttt
    22561 ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag attctgacta acgtgttcat
    22621 tgagctgatc tagtttttct tgcacttttt ggtagagagg aacggttttt ttgatgataa
    22681 aaaataaaat gattgagaaa ataggcaata atgctaagaa aaacagacct acttcccagt
    22741 cgatcacaaa agccatgacc actgacccga tacttaaaaa cggtgctctg atcaataaac
    22801 gaatgaccat tgctaaagct agctgaagct gattgatgtc gtttgtcatt cgggtgatca
    22861 aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa agtattgatt tttttgatta
    22921 gctgattacg taactctgta ccaaatcctt gtgaagctac tgaagcatag tattggcaaa
    22981 tgatagcaca aatcagacca atcacagaca tagcgaccat ccataagccc atttcaatga
    23041 tttttcctcg gtcattcatt tttagtccat tatcgatcaa ggaagccatc attaaaggca
    23101 ggacaagttc gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac
    23161 gatagtcttt tgcataacgt aataactgaa tcatatgatt cctcctatta tttatttgtc
    23221 cacaattgaa aaaacaagta ctaccacatt gtagcttagg aaatttagaa t
//
LOCUS       DAYYFD010000044        23256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000044 DAYYFD010000000
VERSION     DAYYFD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.044"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1372)
                     /gene="glmM"
                     /locus_tag="ACHA25_001603"
     CDS             complement(17..1372)
                     /gene="glmM"
                     /locus_tag="ACHA25_001603"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8762897.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(1396..2565)
                     /locus_tag="ACHA25_001604"
     CDS             complement(1396..2565)
                     /locus_tag="ACHA25_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8762898.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(2562..3449)
                     /gene="cdaA"
                     /locus_tag="ACHA25_001605"
     CDS             complement(2562..3449)
                     /gene="cdaA"
                     /locus_tag="ACHA25_001605"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8762899.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(3638..7318)
                     /gene="nifJ"
                     /locus_tag="ACHA25_001606"
     CDS             complement(3638..7318)
                     /gene="nifJ"
                     /locus_tag="ACHA25_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8762900.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            7516..8037
                     /locus_tag="ACHA25_001607"
     CDS             7516..8037
                     /locus_tag="ACHA25_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8762901.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(8736..9301)
                     /locus_tag="ACHA25_001608"
                     /pseudo
     CDS             complement(8736..9301)
                     /locus_tag="ACHA25_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9480..10136)
                     /locus_tag="ACHA25_001609"
     CDS             complement(9480..10136)
                     /locus_tag="ACHA25_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762902.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(10704..11639)
                     /gene="whiA"
                     /locus_tag="ACHA25_001610"
     CDS             complement(10704..11639)
                     /gene="whiA"
                     /locus_tag="ACHA25_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8762903.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(11673..12671)
                     /gene="yvcK"
                     /locus_tag="ACHA25_001611"
     CDS             complement(11673..12671)
                     /gene="yvcK"
                     /locus_tag="ACHA25_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8762904.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(12668..13552)
                     /gene="rapZ"
                     /locus_tag="ACHA25_001612"
     CDS             complement(12668..13552)
                     /gene="rapZ"
                     /locus_tag="ACHA25_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8762905.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(13687..14418)
                     /locus_tag="ACHA25_001613"
     CDS             complement(13687..14418)
                     /locus_tag="ACHA25_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8762906.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(14422..15687)
                     /locus_tag="ACHA25_001614"
     CDS             complement(14422..15687)
                     /locus_tag="ACHA25_001614"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8762907.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(15950..18766)
                     /gene="uvrA"
                     /locus_tag="ACHA25_001615"
     CDS             complement(15950..18766)
                     /gene="uvrA"
                     /locus_tag="ACHA25_001615"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8762908.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(18776..20770)
                     /gene="uvrB"
                     /locus_tag="ACHA25_001616"
     CDS             complement(18776..20770)
                     /gene="uvrB"
                     /locus_tag="ACHA25_001616"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8762909.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            21029..22531
                     /locus_tag="ACHA25_001617"
     CDS             21029..22531
                     /locus_tag="ACHA25_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8762910.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22533..>23256
                     /locus_tag="ACHA25_001618"
     CDS             22533..>23256
                     /locus_tag="ACHA25_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8762911.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNK"
BASE COUNT         6564 a         5122 c         4031 g         7539 t
ORIGIN      
        1 tttatgaaaa tatctcttaa tccagtccga tttcttcttt gactactgcc gcgattttat
       61 cgacatagta attaactttc tcatcagtag gcgcttctgc catcactcga agtaatggtt
      121 ctgtaccgga aggacgaact aggattcttc cttctgatcc catttcagct tctgcctctt
      181 caatcacttt tttgattgcc ggaacatcca tcgcaccgtt tttatcactt actcggatat
      241 tcactaattt ttgcggatag attgttacct ctgctgcaag ctctgataat ttcttacctg
      301 tctgtttcat gatattcagt aactgaattc ctgaaagcat cccatctcca gttgtgttaa
      361 aatctaagaa aatcatgtgc ccagactgct cgccgccaaa gttataatcg tttttgcgca
      421 tttcttctac aacgtaacga tcaccaactt gtgtaacgac atctttcaaa ccgattcctt
      481 ccaccgcttt gtggaatcct aagttactca tgacagtcgt aacgatcgta tcttttttca
      541 atcgattttt ttctgccaag tatctagcac agataaacat gattttatca ccgtcgacaa
      601 tgtttccaag ttcatccacc gcaataatac gatcgccatc gccatcgaaa gctagtccag
      661 catctgctcc tttttcaacg accatctcag ctaaacgttc tggatgggta gagcctacgc
      721 catcattgat atttaacccg tctggatttg tgcccatggt ataaaaatct gtttcaagat
      781 cggcaaacaa acgattaaca cttgttgaag ttgctccgtt tgctgcatcg acacaaatgg
      841 tcaaaccgga aaggtcaccg ttgatcgttt gtacaaggaa ttgagaatat ttcaataagc
      901 cttctggaaa ttcttctaca gttcccaatc cttcagctga tggacgaggc aattcatcca
      961 cttcagcatc taataatgct tcaatttcta gctcttggtc atctactagt ttaaaaccat
     1021 ctgcaccgaa aaatttgatt ccattatctt gtgctggatt atgagaagca gaaatcatga
     1081 cccctgcgct tgctttttgc aaacgtgtca gataagcaac acctggtgta gagatcacac
     1141 ctagctggaa cacttcgata cccaccgata aaagtcctga aatcagcgct tcttctaaca
     1201 tttgacctga gattcttgta tcccgcccta ctaacacgcg tgggcgtttt ccttcttctt
     1261 catgctggct taggacataa cctccgcacc gtcccagctt aaatgcaagt tctggcgtaa
     1321 gttccttgtt ggcttcaccg cgtacgccgt ctgttccaaa atatttaccc atagttgtaa
     1381 aattactcct ttaacttaat tttctgagga atttgttctg ctcatttgtg ttgattcgct
     1441 tgtttctgat gtttcttctg tacttgtggt cgagctgctt gtcccagatt ccgaactgga
     1501 agaaatggtt gttgtcgatg gttccgttcc tgaatcagca gatgaaatac ttctactcgt
     1561 acttgtattt tcttgtgtcc gactgctcgt cgaagcagag ctactgcttg acgtttgcaa
     1621 cacgggtgta atctccacac taactgattc gggcttgata cttaacccct cttctactgg
     1681 tatttcaatt ttacgagttg ttcgatgatt gatacctgag acattgatcg gtacagcaat
     1741 actgtcaccg atcgatgcaa gcaaatcgct agagccgctt acttctgctt ccagttttga
     1801 aaaactgaaa tcatagcttt ctaccccggc agctggtgtt ccttgctgta tcccataaag
     1861 tcgtaccgac ttgcttggct tgatcgaatc agctctcact gttacttgca ctggatcaga
     1921 gacgatactt aatacttccc ctgctgcatt cagcgcctgt attgggacct cactattaat
     1981 cccatcatct gtgaccatcg aagcgtccac atcagctact actcgtttga tttctgccaa
     2041 tgttttgtca cctgtagtga tagagacttt tttaggacta acggaagtat catcaagttc
     2101 atatccagat ggtgtgaacg ttgaaggaat caacgtttcc acatcgaaat cttttattac
     2161 ctttttctca accgttactg taatagtcga tggttcgatc tttgctgtga cagcactgct
     2221 caaattttga atttttagcg gaacctcatg tgttccttcg cccagcttag tgatatctgc
     2281 tgtgacgcgg aacatccgtg tatcttcgtt tgcctctgca ttcaactgaa ctcggtttgc
     2341 actggaaagt ttgacagtca ctgcattttc atatccatgg atatagtagc gctttgaatc
     2401 ataagaaatc ttgattggca ccccagtaac tgtttgatta tacgcttcat ttccagaaag
     2461 cgtatttgga acggactggc catttgcatt gaagtacagc gcaatactga acagcaatgc
     2521 aataaaactg taaagaatgc ttgtccgttt ctcttttgag atcatttgcg atcccctccc
     2581 ttagtcatac cgtctaggaa agattgaatc acgtttttct tgttttttgt tttctcttca
     2641 gcaatcagtt ctctacgcaa aattgccaaa tattcttgct gggataagcc agccatcagt
     2701 tcattatcta atgtaatgct gacatcccca gtttcttcag agacaacgat ggtaatcgcg
     2761 tcgctgactt cgcttatacc gacagctgcc cggtgtcgag tcccgaattc ttttggaatc
     2821 aacatacttt ctgatagagg aagataggcg cttgctactg cgatctttcc ttcttttaca
     2881 ataactgcac cgtcatgcag cggtgtatta ggaataaaga tattgatcag cagctctcca
     2941 gtgatatctg catctaatgc aattcctgtc tcaatatact catctaatcc agtatgccgt
     3001 tcgatagtaa ttagcgcacc gatttttcgc ttggacatat attggatggc tttgtcaaaa
     3061 gacaagatca tcttctcatc ttcctgctgt tcgctttttg attgtttgaa aagcgaactg
     3121 cgtccaagat gttccagtcc tctccggatt tccggctgaa aaatcacgat agcagctatc
     3181 accccgtacg taatgacttg gttcatcagc caagacaagg tgtgcagacc aatgatttcc
     3241 gctacgattc ggataacaat aaaaatagct acacctttca gaagctgaat cgcttttgtg
     3301 cctcgcagca gttggatcag tttgtataca agataccaga caaccagaat atcaagtatg
     3361 ttcaccaaaa aattcagaga gaacaagtca ccggaaaaca tttgctgcca atagtccaaa
     3421 ttaaaaagtt ccccgatttg aaatgacata aaaagcctct cttctctttt gagatttcct
     3481 ctctagtata tcatatgttt taattttaga ggctgatctg ctcgtatttt tcttgagaaa
     3541 aacgcataat tttcaggaaa attgcaagta tgcgttttat ttttctctgc aataaatatt
     3601 tttaaacttc agagaattat tcattatatt ttttctgtta tttttccttt ttttcttttg
     3661 tagcttgttc cattttttta cgtcgttctg ctctggcagc tcttctttct tctgcttcaa
     3721 ggtccgtcgt tttttctttt ctagtcttaa cgggtacatc gatagattcc tctggttcaa
     3781 gtcgtttctt gattttttct aattctcccg tcagtgcagc atagttgtaa aaacgtgttt
     3841 tagcgtcttg gatagtcttt tcaaataaag cttttgcaag atttggattt gcgttctcta
     3901 gcgaggaaaa gcgaacttgt ttcctcatga aatcaggcat agcagagaaa tcaggttttt
     3961 tgtagtccaa agtcattggg ttcttgtttt tttcgaccat tgcagggtta tatcgatata
     4021 atgaccaata gccagaagaa acagcttctt ttgcttcttt aagcgattct ttcattccac
     4081 caagcaaacc atgtgtgata caaggcgtat aggcaatgat taaagaaggt ccaggatact
     4141 tttctgcttc ttcaaaagcc tttatcgtct gcatttgatt ggcacctgaa gcaatttgcg
     4201 ccacataaac atttccatat gtcatcgcca tcattcctag atctttcttt gaagcatact
     4261 ttccactagc cgaaaattta gcgattgccg aagctggtgt tgcttttgag gtttgaccgc
     4321 cagtattgga atagacttcg ttatccaaaa caagaatatt cacgtctgct ccgctggcaa
     4381 gtacatgatc gatcccgcca aaaccaatgt cgtaagccca tccgtctcca ccaatcatcc
     4441 attggctgtt ttttacgaat agatcctttc tcttgtatat atcctccagt tcagggacaa
     4501 ttgatcgttc gctttcgatc gcagcttgca gttttgctgc tctttgctgc gttccttcgc
     4561 tctcaaacat gtgtaccaac cagtcttcca tcaattctcg tagttcttct gatacttttg
     4621 gcaatacttc tttgattgaa cttgccaaag cctcgcgacg agtctgtgcc gctagaagca
     4681 taccataacc aaattcagca ttatcttcca ataaagaatt tgaccatgca ggcccttgac
     4741 cacattcatt ggtcgtataa ggagaagcag gagaagcacc gccccaaata gaagaacagc
     4801 ctgtcgcatt cgcgatcatc attcgatcac cgaacatttg tgtcaataat tttacataag
     4861 gtgtttcgcc acagcctgag caagcgcccg agaattcaag aagcggttga ttgaattgtg
     4921 tagacaagac agtattcttt cctttgactg gatttgcttt ttgtcttaat gtcattgcaa
     4981 aagcccaatt cacagcttgt tctttctgtt cttcataagg cttcatcact aaagcttttt
     5041 cttttgcggg acaagcctct acacacagac cacaacctgt acagtcttcc actgaaactt
     5101 ggatacggta ttttacgccg tctgcgcctc gcatttctct cacgataaat ccttctggcg
     5161 cttcttccat ttcctcttca tctgctaaaa atggacggat ggcagcatga ggacaaacaa
     5221 aggcacattc attacacatc gtacagcgat cactaatcca ctcaggtact tctaaagcga
     5281 cgccccgctt ttccatagaa gccgttccta aaggcatttc gccagttgtc attccatttg
     5341 ctacaagtgt accaacagag agttgatttc cttcctgtgc attcactggt tcaagtattt
     5401 cttgtatata accagtgttc cttttattga ttttttcagg catctccaac gttctccaat
     5461 ggtctgggat cgcgacttga tgaagcagtt cggtcgtccg atcgattgct tgtatgtttt
     5521 tttctacgat agccattgat ttatgtccat agcttttcag ggcttcttct tttagcagcg
     5581 gaagcacctc atcaaaagga atgatgtcag ctaatttaaa aaaagccgtt tccattgccg
     5641 tattgatccg gcgtcccaaa ccgacttctt gggccaatct catggcattg atcgtataga
     5701 atctaatgtt attttcagcc aagtaacgtt ttaattttaa gggcagatgc gtttctaatt
     5761 gttcgtcgct ccaaagagta ttcaataaaa aaatgccacc tggcttcaat cctttgacca
     5821 agtcatagct atgcaggtaa gctggtgtgt gacaggcaac aaggtccgca tgctcgacaa
     5881 gatacgctga acgtatcggc gtatctccaa aacgaagatg agagacagtc agaccgccag
     5941 attttttcga atcataataa aaatagcctt gcgcatattt atcggtgtga tctccaataa
     6001 tcttgatagc tgatttattt gctccaactg ttccgtcaga tccaaatccc caaaacttgg
     6061 cctggaatgt ttgcggttct gtcaaatcca gactttcctt tggttccaaa gattgataag
     6121 tcacatcatc cacgatccct atggtaaacc gttttttgcg tatgcttggg tcttttttca
     6181 attcatcaaa aactgctgag atttggtcgg gagtcacatc tttagacccc aatccgtagc
     6241 gaccaccaat aacagccggg cgaagttcag aatcatataa cgcactttga acgtctaata
     6301 agagaggttc tccacctgcc cctggttctt tacttcgatc aagaacagca attgttttta
     6361 ctgtatgagg caacttttgc acgaatgttt ccactggaaa aggacgataa agatggatat
     6421 tcaaaaaacc tgttttccgt ccgtttgcat tcaagtaatc aatcgtttgt tcaatcgtct
     6481 gtgcaactga tcccatagaa acaatgactt cttccgcatc gtctgctccg taataagtca
     6541 ccaagtcata agaagttcct cgtaatcggt tgatctcttt catatatttt tgaacgattt
     6601 caggtaattt atcataatag gggttgatcg tttcccgttg ttgaaaatgg atgtctggat
     6661 tctggttcgt accactaaca gtcggatgat tgggattcat ccctcgttta cgaaatgaag
     6721 ctaatttctc ctgattgatc agaggagcta attcttcata ttccaaaact tcgatttttt
     6781 gaatctcgtg actcgtacgg aatccatcaa agaaattcaa aaaaggtact tctccttcaa
     6841 tagaagcaag atgggcaaca ggtgctaaat ccatcacttc ttgtacactg ctttcagcta
     6901 acatagcaaa tcctgtttgt cttgcagcca tcacgtctcc gtgatctccg aaaatattca
     6961 acgcatttgt cgtcactgcc cgactagcaa catggaaaac acttggcagt agttccccag
     7021 caatcttgta catattcggt atcatcaaaa gcagcccttg agaagcggta tatgtagttg
     7081 tcagtgcacc agttttcaat gatccatgga ccacaccagc tgctcctgct tcggattgca
     7141 tttctactac ttttactggt tgaccgaata cattttcttt tcctgctgct gaccattgat
     7201 ctactaattc agccatggtc gaactgggtg ttattggata tattgcagca atctcggtaa
     7261 atgcataaga aatatatgcg gctgccgtat tgccatccat tgttttcatt tttcgcatga
     7321 ttcatcatcc ttgttcatat tctctcttat tatctgttcc attataacgt aaaacattcc
     7381 attttttctt ttatttataa aaatatttca catatgtcta tttgaattgg tcatctaatt
     7441 tttagacaaa cagtctaaat cgttgccttt ttttgtcaga catgtataaa ctataagcga
     7501 aagaggtgtt ttaacatgga tatatcagtg attgatgcaa cgaaaacaaa tacacaaaaa
     7561 ggaatccttt atggttcatc gaatgctcct aaaaaaatgg tcgaattcat caacctatct
     7621 tgtccgtatt gccgacaatg gtttgaagaa tcttatgagc tattagaaga agcagtccaa
     7681 agcgggcaat tgcagcgagt gatcaaattg ttcgataaag aaaaagaaag cttgcagcga
     7741 ggaaacgtga tgcaccggta tctcacaatc agcgatggtc aaaaagcgat caaagagatc
     7801 aaacaaatat tcgatactca agacgagtgg aaacatcttt ctctccaaga agtagcggat
     7861 tttgcagtag ataaactaaa gctgacagaa caaaaagacg aacagctgag ccaagctgtc
     7921 atcaacgaag ctgagcaagc acatattcga tttgttccga ctgtcatctt gggtaaagaa
     7981 atctttgatg aatctatttc aatagaggaa ttaaaagaat tgatccaaac aaaataaaaa
     8041 aataaggagc gtaggacaaa agtaaagaac acttttgtct cacgctctaa acacctataa
     8101 acggcggaag cagaagcaac tccttcttat ttcttggagt taaacgcttc tgtcccagcc
     8161 tccttattcc gaataaatgg tgtccaaaag cttgcatctg ccagattatc tcatttctta
     8221 tgacaagctg agcaaattat ctccaccaaa taatttattc atatttttga cttgcgcata
     8281 ctaactaatt cttaaatttt catacaaaat ttctcaaatt cccttaacga gtaatattta
     8341 tgtacggtga ttcacactag tatcccaatc actttttaag ttatcccgat ttcaattcta
     8401 cttcaaaaat agttgctttt atgtatgctg ttagttgatt agatggtcgt ttgttgagct
     8461 aggtcgttta ttttaaacaa gttgtttctt tagcttatac ctatctcgtc taccaaacgc
     8521 aagatggcaa acacaaatgc atacgcatct gtatttgcca ctttgattcg tataaagcta
     8581 acaactccta actcctctca tttaatagtt gctctctttt ttattcagat acatttttgc
     8641 tgatcacagc ctagtatttc aatggttttg aacactaaac caaattgttc cattacaaca
     8701 ttcactggct gatgaaatgg attaatagat ttcgattatt caaagcgatt ttctattggt
     8761 ggattgtgtt cccaactaac aagacggatt ccaaagtcat tatataactc ttcaagagtt
     8821 aaccctttcc tgttcccaat agatgtatct ctcccacgct cgatataaaa tatggagttt
     8881 cgaatgccaa gacctggcaa atctttcctt gttacagtta cactgctact aacttcccca
     8941 tataaaatcc agctcctcca aaatgataat atatttttcg accatcatct aactcatgta
     9001 acaataattg ttgtgccctc ttgtcactcg ccaaatgcaa acgccacgtg cgataccatg
     9061 ccatgctacc tgcatctgca gaaaatccat caaataatgc aacaattgta tcttctatca
     9121 caatcctttc cccatctact tcatattcta atctaaaatc aaactcccct cgggtaatcg
     9181 caggtcttgg tggcttagga cccaatatta ttctacttaa aaccgcattc gttcccagat
     9241 gaaacacagc tataacaaca ataatgatta atattttgtt tctatttgat aaatttctca
     9301 ttattcgcaa atcacctcta cattctcaca tactagttcg taatactcct ttaatatcaa
     9361 aatatctact gatcgtaata atattcgcta tgaattccta ctactcgtat aagaaaggcc
     9421 aaatcatcag cttttacttg ctgatgattt ggcttgtttc tatttatcgt aattgttttc
     9481 taatcaccaa aattgttaac aatcggttca gatatttccc aattcactag ccttatatta
     9541 tattgattta acaattcttc ttcatttatt gtattttgat caagcggacc aaattgttct
     9601 aataattctt tgtcatgtaa aatatccctt ctagtaatgc ctctttccct ttcaatgaaa
     9661 gaagcactag gaaaagtatg attataacca ttcaacctat ctcccatgta ataattagca
     9721 ggtcctggaa aataaaatat ttgcttatta tcactaactt ctaacagcaa cactaaatct
     9781 tctccgctgg ctaaacgact tgtccatcta cgatatctcc ctatgccttc gttcattcca
     9841 aatccatcaa attcaactat taacgtgtct tctatcacat gtaattcttc attcaattca
     9901 tactcaagtc taaaaggaaa ttctccatat gtaatctctg gcaatggtgg tgctggagtt
     9961 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
    10021 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
    10081 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa acgcattttt
    10141 gccctccctt tataaacatc gattctcaaa ttctaaacca aatcttggta gttcaagttc
    10201 aggtataacg tattccaacc atacatctca attactattt atgaacttat gattatcact
    10261 gaataaatca ctccatcccc ttgttaaaac ttctacatta attacacctg gttgttttcg
    10321 actagctaat ccaatgaact tatcaaccat ccttatccac caaccacgag aatcttgacc
    10381 agaagtaaga agagcagttc tcaaggatga tcgagcagtt ccttccagtt ttttaattaa
    10441 tcaggccagt tatagataaa tacttgatct tatctatatc aggacttcta tattaagcaa
    10501 aaatttatgt acaaaactaa aatcttcatt cagttgtctc cccgcttttt atacgattgt
    10561 attaaaaaca atagaagcct ttccaaatta tgttactcca gtaaagatgg aattatagtt
    10621 tgacggaaga aaagcaagta taacttttaa aaaaaactga tacattcgca tctcctgctt
    10681 catgtatcag ttttttcttt ggattaaact gcttcttcac gcagtttttc cgcaaattca
    10741 tttattttgc gaattcggtg attaattccc gattttgata tcgcacctga gggaatcatt
    10801 tcccctaatt ctttcaagct gacctctgga tgttgaagcc gaagttctgc gatttcctga
    10861 agtttttcag gaagggcatg tagaccgacc tttgcttcga tcaattcgat gttttcgatt
    10921 tgtttagaag ccgcatcaat cgtcttgttc agatttgctg tctcacaatt gactagacga
    10981 ttgaccgaat ttctcatatc ccgtacgatt cgaacatctt caaatcggag cattgagttg
    11041 gttgcaccaa tcaatgttaa gaaatcagca attttttctg ctccttttaa ataagaaata
    11101 tagccattgc gccgttcaag cgttcgagca tgtagaccgt aataatttaa catatcgcaa
    11161 atgtcttggt tatgctgttc ataaatagaa aatatttcca aatgataacg gctcgtttcc
    11221 ggattattga ctgaacctga ggccataaaa gcaccacgta agtaagaacg cattttttgc
    11281 gaatttccca taatctcgtc cgaaacgtgt ccattgaaca tcataccatc catgatatca
    11341 agatccatca acacatcttt tgtgccttgc ttcaaccgga cgatatagac attgttcttt
    11401 tttaatttca tttttttacg aacgagtagc tcacttcgta cctgataatg atctttcaaa
    11461 agagaataga tacgtctggc aattgcagca ttttctgttt ggacgttcaa aataaactgc
    11521 tggttcacaa tacctaaaga gccgttcatt cgcagcaaag cagctaattc cgctttcgca
    11581 tgttctcgat gaacttccaa accagttagt tctttcttca catctgcagc aaatgacatc
    11641 ctattctcct cctttctcca acaataaatt gcttaatatt ttgaaccgaa aactaaacgg
    11701 aacaattctt ctactacttt gtctccatcg tggaagacac caccatcttt caattccaaa
    11761 aagtcggtgg aaatcactcg acatccttca tttctcaatc cgttgaagtc gtgtgcgact
    11821 tgtactagat actcatcata gatctctggg tccatataat cttcaggcac tttttccgta
    11881 ttgaccaata ctgtatcgat aaaaggctga ttcaaatgct tgtgtaaaac acgtatatga
    11941 tcggcatctg tgaaatgttc cgtttcccct ttttgggtca tgatattaca gatatagacg
    12001 gtttcagcag ttgtcttgag cattgcttcg ccgatttctg gtatcactaa atttggaagg
    12061 atactggtga ataaacttcc gggtcccaaa acgaccatgt cggcgtccat aattgctgaa
    12121 acgacttttc gagccgcttt ggcttcactt ttatcatttg tgttgcagac atacacacgg
    12181 tcaatggttt tacggtcgat cgcgatttta gattctccta ctgcagtcgt tccgtcttta
    12241 aagacagcat gcaaggtcaa aggagtttct gaagaaggat aaacatgtcc atctacatgc
    12301 atcatttttg ctaataattg aatcgcctca taagtgcttc cccgcatctc ggaaactgcg
    12361 gcaatgatca gattaccgat cgtatgattg gctaaaaact gatcttcctt tttgaaacgg
    12421 tattggaaaa tatcttcata gaattgtggc atatccgata acgccactaa gacatttctc
    12481 aagtcaccag gaggagccat agcgatcgat tctctgattg caccgctgct tcccccatca
    12541 tctgcaacag tcactactgc tgtgatatct gctccttgat ttcgcaaact tttcaggata
    12601 actggcagac cagtgccacc gccgaccact acgatctttg gttttcttat ccggtaggtt
    12661 ttcattttca cgaccggttc accgtctctt tccttttatc cttgtcccga tgtgtcacat
    12721 tgactttata gtttttccct aaagcatttg ccaaacgttc agtcaatgcc acagaacggt
    12781 gttgaccgcc tgtacagccg attgcaactg tcaagctgct ttttccttct ttaatgtatc
    12841 caggcaatac agtttccaga agatccaaaa atttatggta gaatgattgc gtttcatcaa
    12901 aggacatcac ataatcatag actggctgat cttttccggt caatggtctt agttccggga
    12961 tataatgagg gttgggcaaa aaacgcacat ccatcacaat atccgaatcg atcggcaacc
    13021 cgtatttaaa accaaatgat acaaattcga tccgaaatcc ttggtctccc tttgatttga
    13081 atgactcatt gattttttca cgtaattgtc gtggtgtcaa tgtcgtcgta tcaatgatca
    13141 cggaagcttg ggctttcaag tcgtctagta tcgcccgttc tttacgtatc ccttcagtga
    13201 tcaaaccgtc catcgccatt ggatgtgctc gtcttgtttc tttgtagcga gccactagtt
    13261 cttcgtctga agcatctaaa aataagatag aagtgtcgat gaagttcgta ttttcgattt
    13321 cgatcagcat gttttggatt tcttcaaaga aggaacgaga acgtaaatcc actcctaaag
    13381 ccatttttgt gactttgcct gattctttga ttaattccca gaattttgga atcaatcgag
    13441 gcggcatatt atcgatacaa aaatagccca tatcttcaaa actttggata gctactgttt
    13501 tccctgcacc gctcatacct gttatgatga ctaattgcaa attatctgtc ataattacga
    13561 actcctctca cattgtgcat agtaaaatta tatcattccg ggcgtatcat taccagtttt
    13621 ctttttcttt tacaaaaaga tttggaccaa atatctttta ggatatttgc cccaaatcca
    13681 atttttttac ttttccgatg ctgtatccaa tgccttgctt ctttcagcta gcgctcgttc
    13741 aatcgtccga tctgctaaaa tagattgggc atcgatcact ggataatggt tatcttcagc
    13801 aaattcatgc atcaatgaca attctgtgca acctaagata actttttcac agtttaggcg
    13861 ctctaccgct tcttctaaga tttcgtaata tagctcttga ttcaaataat ccgattcctt
    13921 gatctcatga tagatcagat aattgatttt ttcttgaaga acagtatcag gaatcactgt
    13981 ttcgaatcca agatttttga cttcgcgttc atagattcca gctttcatac ttccttctgt
    14041 tcctagaatc gctactctcc ctgtcgtatg ctgacggaca agttcatttg ctgcttctct
    14101 tggcatatga aggataggaa tatccgttgc cgcttgtaat tcttcaaaaa agtagtgtgc
    14161 ggtgttacaa gtcaacacaa tgaagttcgg ctgcaataaa ttttgtttct caatatcatc
    14221 cagcagaaac ggcattggat tttcttctga tcgatctaaa atataagctg tacgatcagg
    14281 aactgttgca tgattgaata agacataatt caaatattct tgatcttttg tagctttcgt
    14341 tcggtggttg atcaagcgaa caaaactttc tgtcgccatc gtgcccattc cgcctaaaat
    14401 actgaaaaaa ttctccatga actagccttc tttcacatga aaatactttt tgaatcttgg
    14461 aatatagtta tggaacatat atttcatcaa caaccagcgc ttgattgacc ggtctttttc
    14521 ataaaacaca gtagtaccgt aacgattttc tttgatcatt tgctcagcta gttttttgtc
    14581 ttcattttcc cgagcatatt ctaaaaagat ttttttgggc acacccatcc ataatcttgc
    14641 tttatctgat tgattcgttc cataggtcgt ttcaacaaag ggtttgttaa acactcgatc
    14701 ttctgtgacg aaacgagcca gattcaagcc gttcaatgtc acaaagaaac tgctacgacc
    14761 ttgtctcaaa ttgatttcaa ataatttata ttctccatct ctaggatcat acttcatatc
    14821 gaaattggca aagcctgtat attcgatttt ttccaaaaat gccttaatgg tttgatagat
    14881 tttttcgtta taatctggca tgatcacaac gtaattacca atcgatgctg gtgtaggatc
    14941 ttctaacagt ggatgaccaa gacacatcat ccgaacttga tgatcttcat ctacatacgc
    15001 atttagcaca cgcatattac tatcatcacc cgggataaaa tcttgtacaa tcatttcact
    15061 tttataaccg gcttcgtata tccggcctaa aattaaatcg aactcttctc tagtctctaa
    15121 aataaaagct ttttttcttc cttcaaattg gacagaaaga tactccacac tgtttgcagg
    15181 tttcaaagca actgggaatt caaaaggcaa ttcctgttcc aaatgtccat ttactaacat
    15241 ttcttctctg acgatcaatg ttttaggata cggaagatca tattcttcac aaacttcata
    15301 aaagcttact ttattgatca gtctttcaaa taaagaataa tcaatatatg gacagataaa
    15361 atattctgac agctcttgtt tatgttggga gatcaattct gcatatccat caccacaagc
    15421 aatcaaaaga tattttttac tcttatcaga atatctttct tttgccaaac gaagcatcgt
    15481 ctcgataaac acagggtctt tatcaaatcc tggaataact tctacattga caattttact
    15541 gtagcgggta ggggctaact gatccgaagc atatgcttgg cagattttgc cgtatgcttc
    15601 attaaaagaa cgtgccatac cataaacgtt catgtcgctt cctaataata tagggataaa
    15661 ttcttcattt tcaatactgt tcatcattat ctctcactta ctcataatct cttgtttttc
    15721 ttttccaact acacaaggaa tatcatacca taaagaaaaa agcaatcaaa ggaaactcct
    15781 ccgattgctc aattcttttt tgaacggatt gaacttatag gaagtttttg tctataccaa
    15841 tcagtgtaga aagaactacc atgtaatgat tctagaacac tgatattaat aaaacaagca
    15901 aaaattcatt tttttatgtt tttttaacta tctcagctat ctatcctctt tataccatta
    15961 tttttttcaa atagtgacct gtataacttt cttttacgtt aatgatttct tctggtgttc
    16021 cagtcgcaag gatcgttcct ccaccttctc caccttctgg tcctaaatca atgatataat
    16081 ctgctgtttt gattacatct agattgtgtt caatcactaa aactgtgtta ccagcatcta
    16141 ctaatctttg taatacatgc aacaagcggg cgatgtcatc gctatgaagt cctgtcgttg
    16201 gttcatctag tatatagaaa ttctttccat tagagatttt gtgcaattca ctggcaagtt
    16261 tcatccgttg tgcctcacca ccggacaatg tcgttgctgg ttgccccatc gtcacataac
    16321 ctaagccaac atcaacaatc gtttgcagtt tgcgatgaat ctttggaatg tgcttgaaga
    16381 attctacagc atcttctact gtcatttcca aaatatcagc aatgcttttt cctttgtaat
    16441 gcacttctaa tgtttcagag ttatatcgtt tgccatgaca tacttcacaa ggaacataga
    16501 catcaggcaa aaagtgcatt tcgatcttaa taattccatc cccgcgacaa gcttcacaac
    16561 gaccgccttt tacgttgaaa ctaaaacgcc ctttcttata accccgcatt tttgcctcgt
    16621 tcgtttgagc aaataaccca cggatatcat caaatacact tgtataagtc gctggattac
    16681 ttctcggcgt ccggccgatt gggctttgat cgatatcgat gatcttttcg atactttcgt
    16741 agccggaaat tgtcttgaat ttacctggct tagcagaatt cttatttaat ttttgcgcta
    16801 acgatttctt taagatacta ttgaccaatg tactctttcc agaaccagac acacctgtga
    16861 ctgctacaaa ttctcctaat ggaaattcaa cactaatatt tttcagattg ttttctgaag
    16921 caccagtgat cttgattgct ttcccattac ctttacggcg ttcttttggt acagggattg
    16981 attttttccc tgaaagatat ttccctgtca aagaattttc atcattagct acttcttcag
    17041 gagtgcctgc agccacgatt tgtccgcctt gttctccggc cccaggtccg acgtcaatca
    17101 gataatctgc tgcccacatc gtatcttcat catgttcgac tacaacgagg gtgttgccaa
    17161 gatcacgcat tttctttaat gagccaatca gtcgatcgtt gtcgcgttga tgcaatccga
    17221 tcgatggttc atctaaaata tacaaaactc cagaaagatt cgatccaatt tgtgtagcca
    17281 atctgattcg ctgcgcttct cccccagata atgttccgga agcgcgactc aaagttagat
    17341 agtctagtcc cacattttcc aagaatgaaa gtctgtcatt tacttctttt aaaatcggtt
    17401 tggcaattac tttctcttgt tcagataacg ttaagtttga gaagaaatct actgcttttt
    17461 gaatagacaa ctcatttgtt tgcccgatat tcgttccatt gatttgaaca gatagagctt
    17521 ggggattcaa tcgataacct ttacatgtct gacaagttaa ttctgtcata taaagacgca
    17581 tttgctcacg cgtgaaatca ctgttcgtat catgataacg acgctcgata ttcttcaata
    17641 ccccttcgaa agtcgtttct acatcgcgaa cgccaccaaa atcattttca taatggaaat
    17701 ggaaaagatc atctgtccca ttcaagatga tatcctgatg ctcttccggt aattcttcga
    17761 atggtgtatc taaatcgata ccgaaacttt cacaagcctg cgctaacatt tgaggataat
    17821 actgagagct gattggattc catggaacga tagcaccttg actaagtgtc ttcgttctat
    17881 ctggaatcac taaatcttga tcgacttcta atttgacacc taaaccatca cagtctggac
    17941 aagctccaaa aggcgcattg aatgagaaca gtcttggttc tagttcgcca acagtaaatc
    18001 cgcaataagg acaggcatag tgttcgctaa acaacatttc ttcttcaccg atcacgtcaa
    18061 ccagagcata gccgtctgcc aatcgcagag ccgcctctaa agaatcaaat agacgagaac
    18121 gaatcccttc tttcacaatg atccggtcga tgacgatcgc gatatcatgt tttttgtttt
    18181 tctccagctc aggcacttca cttaaatcat agatctcacc atcgactcga acgcggacgt
    18241 atccctcttt ttggattcgt tcgaatattt ttttatgttg tccttttttc tttaccacga
    18301 tcggtgccaa cacttggatc tttgtgcgct caggcaaagc cattacttga tctgccattt
    18361 gatctactga ttgactggta atctcgatat gatcgttggg acaaatcgga tgtcctacac
    18421 gtgcaaacaa caagcgtaaa tagtcattga tttccgtcac agtacctact gtcgaccgag
    18481 gatttttact tgttgttttc tgatcaatag aaatcgccgg actcaatccg tcgatgctat
    18541 ctacgtccgg cttatccatt tgtcctaaaa actgacgagc ataagcagaa aggctctcta
    18601 cgtatctccg ctgcccttca gcgtacagcg tatcaaatgc tagggaactt tttccagaac
    18661 cggacaaacc tgtaacgacc acgaatttat ctcgtggaat cgttacatct atatttttca
    18721 agttatgggc acgtgcaccg tgaatcgtaa ttttatcgtt agccatgttg tcctcctact
    18781 ttgaagcttt taattctaaa atcatatctc gcacattagc ggctgtttca aaatccaatg
    18841 ctttcgccgc atttttcatt tctttttcta atttcatcaa taacgtatct ttttcttctt
    18901 tcgtcatttc ttcatacgag acttgtaccg cttcttttgt atcatcttcc gattctttcg
    18961 taatagaaat caaatctcgg atttctttaa tgatcgtttt tggaacgatc ccatgttctt
    19021 cattgtactt ctgctgaatc gtacggcgtc ttgatgtttc atccatcgct agacgcatag
    19081 aatctgttac tttgtctgcg tacatgatta ctttaccttc ttcattccga gcagctcgtc
    19141 caatcgtttg gactaaggaa cgttcactac gtaaaaagcc ttctttatcc gcatccaaga
    19201 tagcaacaag ggaaacttca ggtacgtcta atccttcacg aagcaaattg attccaacta
    19261 acacatcaaa ttcacctaat cgaagatctc gaatgatttc tgttcgttcc agcgttttga
    19321 tatcactatg caaatatttg actttgatcc ctaattcttt gaaataatct gtgaggtctt
    19381 ctgccatttt tttcgtcaat gtcgttacaa acacccgctg gtctttttcg actcgttcat
    19441 ttatttcgcc tactagatca tcgatttgtc ccatgattgg acgaacttcg atcactgggt
    19501 caagcagccc tgtcggacgg atgatttgtt ggataacggt atctgtttgt tcatgttcat
    19561 atggtcctgg cgtcgcagag acataaatga tctgattgac atgtttttca aactcttcta
    19621 accgaagtgg ccgattgtct aaagcagatg gcaaacggaa accgtaatct actagcatct
    19681 gttttctcgc tcggtcacca ttatacattc ctctgacttg aggcatggtt acgtgcgatt
    19741 cgtccgccac gatcaaaaaa tcttcaggaa agaaatccaa tagcgtataa ggcggttcac
    19801 cttctttacg tccgtccata tgccgagaat aattttcaat tcccgacgtg tagcccattt
    19861 ctaacatcat ttcaatatcg taattggttc gctgttctaa acgttgagct tccagaagtt
    19921 tgttttcatt tcgcaagaca gttaaacgct gttctaattc cgctttgata ttcgctacgg
    19981 catgctccat atgttcatca ttcgtcacaa agtgagtcgc cgggaagatt gccacgtgtt
    20041 ccgtttctcc taaaacttca cctgtcagtg cattgacttc tcggatacgt tcgacctcat
    20101 ctccaaaaaa ctccacacgt aaggcacgtt catctcttga agccggaaaa atctccacga
    20161 catcaccgcg cactcgaaaa cgaccgcgct gaaaatctat atcatttctt tcgaattgga
    20221 tactgactaa gtcacgaatc aactgattgc gatctagttc tgctccctgt cgaatcgaaa
    20281 ctacttgttt ttggtactca aaaggcgaac ctaatccaaa aatacatgaa acggatgcaa
    20341 tgacgatcac atcatttctt tctaataatg aactagtagc cgaatgtcgt aatttgtcga
    20401 tctcatcatt tacacttgaa tctttttcga tatacgtatc gcttgaaggc acataagctt
    20461 caggctgata ataatcataa taagaaacaa aatattcaac agcattgtta gggaaaaatt
    20521 ccttgaattc cccgtacagc tgaccagcca atgttttgtt atgggcaata attagtgttg
    20581 gtttattcac tttctcaata agattggaaa tcgtatatgt tttccctgtt ccagtcgcac
    20641 ctaataagat ttgtgctttt ttcccgccga ctacgccatc cactaattgg ttaatcgctt
    20701 caggctgatc accagccggt tggtatttcg agacaagctc aaagtgtcgg gaggtatctc
    20761 tctcaatcat tcttcgtcct ccttttcatt cattcctttt cattttacga ccaataaaaa
    20821 gaaaaggaaa gcaaaggcat tccttactaa aaaattatac cacaccgaac atactttcgc
    20881 cactttttta tcttattatg catgttacgt tttataacat aaaatgttag aaactcgcat
    20941 ttttttcatt ttttcgagct tacagtcttg tctgattcag cacttttcgc ctataataat
    21001 aaaatattgg aaatttagga ggttgattat gaaaagaaag catttatttc actcatttgt
    21061 tttcttaatg acaggacttg tgacacttgc gtttggacat gttgcacaag ctgacaacac
    21121 tacaacggca gagtcaagca caagcgagac aatgcaaaaa atagagccca aaaaagacgt
    21181 atacgtaatt gccagtgact ctgcttttgc tccatttgaa tttcaaaacg caaagggcga
    21241 gtacgaaggc atcgatgtaa aattgatgga aaacatcgcc aaactgcaag gatttaacat
    21301 ccgaatggac taccgtggtt tctctgctgc tttgcaagct ttggaatcag gacaagcaga
    21361 cggaatgatt gccggaatga ctgtaacaga tgaacgaaag aaaagctatg atttttctga
    21421 ttcttacttt gatagcggga tccaattagc tgtcaaaaaa ggagatactt cgatcaagtc
    21481 ttatgaagat ttgaaaggaa aaacagtcgg agcaaaagtt ggtaccgaaa gtgctgaatt
    21541 tttaagcgac catgaatctg aatatggcta ttctatcaaa tattttgatg ctgctgatca
    21601 attgtacgac gggttaaaag ttggcgcaat cgatgcaatg atggatgatt atccggtaat
    21661 cggttatgcg atcaaacaag gacaagaatt ggaaacaccg atcaaaaaag aatctggcgg
    21721 aaaatatgca tttgccgtta aaaaaggaca aaatcctgag cttcttaata tgttcaatga
    21781 aggattgaaa gaattaaaaa gaacaggcga atacgataag attattaaag aatatgtagc
    21841 ggatggttca gaaacttcca gtgagtcttc cactgctgat gaatcaacat ttcttggact
    21901 tatccaaaac aactatcaag cattgttgaa aggactttgg agaacaattc tcctaacttt
    21961 gatctccttt gctttggcta tagtaatcgg tgtgattttt ggattattca gtgtagcacc
    22021 aatacgcgct cttcgcacaa ttgctagtat ttatgtcgat atcatccgag gaattccaat
    22081 gatggttctt gcgttcttta ttttctttgg gttgcctggt atcgttggat ttacgattcc
    22141 tgacttcctt gctggtgtta tcaccttgac tcttaacgca agtgcttata tcgcagagat
    22201 tgttcgaggc gggatcaacg ccgttcctgt cggacaaatg gaagcttcca gaagtttagg
    22261 tctttcttat aatcggacaa tgcaaaaaat cattttgccg caagcgatac gcatcatgat
    22321 cccatcattc gtcaatcagt tcgttatctc attaaaagac acaacgatca tttccgcaat
    22381 cggtgtagtc gaattgctgc aaactggtaa gatcattgtt gcacgtacaa cacaaagtac
    22441 gtatgtttac ttgatcattg cgatcatgta cttgattttg atcacagcat tgacaaaatt
    22501 agcaaaagtt ttagaaaaga aggtgaagta agatggcaga aaaaatctta gttgagcatt
    22561 tagtcaaaaa atacggcgat aacacagtat taaacgacat caatgtgtcc atcaacgaag
    22621 gagatgtcgt ctgtatcatt ggcccttcag gttcaggaaa aagtacattt ttgcgttgct
    22681 tgaatcgttt agaagaacct tctgacggtg aaattattat tgacggcgcc cacttgatgg
    22741 ataaaagcac agatatcaac ttggtgagac aacatatcgg aatggtattc cagcacttta
    22801 acctattccc tcatctttct attttggaaa acatcatttt agcaccgatg gacctgaaga
    22861 aagaatctag agaagaagca gagaaaaaag caatcaaatt gctgcagaca gttggtctag
    22921 aagataaaaa agatatgtat cctgaaatgc tttccggtgg acaaaagcag cgtgtggcta
    22981 ttgctcgtgc tctagcgatg aaccctgaca tcatgctttt tgatgagcct acatctgcac
    23041 tcgaccccga aatggtcggt gatgtcttga acgtcatgaa aaagttagca gaacaaggta
    23101 tgacgatggt tatcgttacg cacgaaatgg gcttcgctaa agaagtggcc aatcgtgtca
    23161 tgttcattga cgacggaaac tttttagaag acggaacgcc tcaacaaatc tttgaaaatc
    23221 cgcaaaatga acggacaaaa gatttcttga ataaag
//
LOCUS       DAYYFD010000045        22819 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000045 DAYYFD010000000
VERSION     DAYYFD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.045"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACHA25_001619"
     CDS             1..369
                     /locus_tag="ACHA25_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8762912.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACHA25_001620"
     CDS             549..1490
                     /locus_tag="ACHA25_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8762913.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACHA25_001621"
     CDS             1559..2290
                     /locus_tag="ACHA25_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8762914.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACHA25_001622"
     CDS             2434..4614
                     /locus_tag="ACHA25_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8762915.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACHA25_001623"
     CDS             4634..5116
                     /locus_tag="ACHA25_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8762916.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACHA25_001624"
     CDS             5132..5893
                     /locus_tag="ACHA25_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8762917.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACHA25_001625"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACHA25_001625"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8762918.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACHA25_001626"
     CDS             7665..8150
                     /locus_tag="ACHA25_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8762919.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACHA25_001627"
     CDS             8143..8826
                     /locus_tag="ACHA25_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8762920.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACHA25_001628"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACHA25_001628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8762921.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACHA25_001629"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACHA25_001629"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8762922.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACHA25_001630"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACHA25_001630"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8762923.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACHA25_001631"
     CDS             13123..13455
                     /locus_tag="ACHA25_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8762924.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHA25_001632"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHA25_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8762925.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACHA25_001633"
     CDS             14281..15654
                     /locus_tag="ACHA25_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8762926.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHA25_001634"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHA25_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8762927.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACHA25_001635"
     CDS             16709..17149
                     /locus_tag="ACHA25_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8762928.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="ACHA25_001636"
     CDS             17293..17994
                     /locus_tag="ACHA25_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8762929.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="ACHA25_001637"
     CDS             17991..19112
                     /locus_tag="ACHA25_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8762930.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="ACHA25_001638"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="ACHA25_001638"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8762931.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="ACHA25_001639"
     CDS             20491..21399
                     /locus_tag="ACHA25_001639"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8762932.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="ACHA25_001640"
     CDS             21434..21706
                     /locus_tag="ACHA25_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8762933.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="ACHA25_001641"
     CDS             21717..22763
                     /locus_tag="ACHA25_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8762934.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7694 a         3980 c         5010 g         6135 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 ttaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataaga
//
LOCUS       DAYYFD010000046        22809 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000046 DAYYFD010000000
VERSION     DAYYFD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.046"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..3468
                     /locus_tag="ACHA25_001642"
     CDS             190..3468
                     /locus_tag="ACHA25_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8762935.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(4009..5916)
                     /locus_tag="ACHA25_001643"
     CDS             complement(4009..5916)
                     /locus_tag="ACHA25_001643"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8762936.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6046..6921
                     /locus_tag="ACHA25_001644"
     CDS             6046..6921
                     /locus_tag="ACHA25_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8762937.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7001..7975
                     /locus_tag="ACHA25_001645"
     CDS             7001..7975
                     /locus_tag="ACHA25_001645"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8762938.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8155..9300
                     /locus_tag="ACHA25_001646"
     CDS             8155..9300
                     /locus_tag="ACHA25_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8762939.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9437..9781
                     /locus_tag="ACHA25_001647"
     CDS             9437..9781
                     /locus_tag="ACHA25_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8762940.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10052..11338
                     /gene="efmA"
                     /locus_tag="ACHA25_001648"
     CDS             10052..11338
                     /gene="efmA"
                     /locus_tag="ACHA25_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8762941.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11476..12075)
                     /locus_tag="ACHA25_001649"
     CDS             complement(11476..12075)
                     /locus_tag="ACHA25_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8762942.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12479..13600
                     /gene="mnmA"
                     /locus_tag="ACHA25_001650"
     CDS             12479..13600
                     /gene="mnmA"
                     /locus_tag="ACHA25_001650"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8762943.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13846..15267
                     /gene="arcD"
                     /locus_tag="ACHA25_001651"
     CDS             13846..15267
                     /gene="arcD"
                     /locus_tag="ACHA25_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8762944.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15281..16399
                     /locus_tag="ACHA25_001652"
     CDS             15281..16399
                     /locus_tag="ACHA25_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8762945.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16459..17751)
                     /locus_tag="ACHA25_001653"
     CDS             complement(16459..17751)
                     /locus_tag="ACHA25_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8762946.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17901..18461
                     /locus_tag="ACHA25_001654"
     CDS             17901..18461
                     /locus_tag="ACHA25_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8762947.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18590..18967
                     /locus_tag="ACHA25_001655"
     CDS             18590..18967
                     /locus_tag="ACHA25_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8762948.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18900..20972
                     /locus_tag="ACHA25_001656"
     CDS             18900..20972
                     /locus_tag="ACHA25_001656"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8762949.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21183..22130)
                     /locus_tag="ACHA25_001657"
     CDS             complement(21183..22130)
                     /locus_tag="ACHA25_001657"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8762950.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22376..>22809
                     /locus_tag="ACHA25_001658"
     CDS             22376..>22809
                     /locus_tag="ACHA25_001658"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8762951.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7387 a         4155 c         4818 g         6449 t
ORIGIN      
        1 tttttatacg ttattttaaa aaaacttatt ctttttacgt taataaatct ctttattatc
       61 aacaacttaa gttatatttt ttctttttat aaaatacact tattataatt ttgtttttta
      121 ttgacagtaa aaataaatta ggtaatactt taagtaaacg ctttctaatt tgttacggga
      181 ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa tctccttatg
      241 ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg
      301 aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa caatggagga aataccaaga
      361 actagtgaag atatggatac ggaagaagga aatgatccta tgatgaaaga atcagaaaat
      421 gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt tgttttcgga
      481 gaaagcacaa gtgctagtag gtatgccatt gaaccattag aagacggcgt gcggctctct
      541 tccacgaata acggcggaaa attccagtca agcggatcag atggaatgac ctattattat
      601 caagcaatcc ccaaagacat caactttagg atgcgggcga cgatcgaaat agaatcttgg
      661 acctatacga atgcccaaga aggatttgct ttaatggtaa gagacgcagt accaaaaaat
      721 catttgtttg ggcaggcatt ttattcaaat tcatttgcca tactaggcag tcggatcgaa
      781 tatgtctggg actcggatag acaagaagtg gtcaacacga gcggcagcaa atatacgatg
      841 cgacttggat tggggacacg ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg
      901 ccagctcctg ggagtgtctc agtggaaaca acacctttag agacttcagc agggttcttg
      961 gaaaaagaaa caggttctta taatatattc ggaaacggtc atgggaatct gtttccggcc
     1021 acgaactcga ttactaaact ggacgtagag atgaaaaaaa cgaatactgg tttagaggca
     1081 tattattacg atcctgaaac aggagaagaa atggcttctg acatcatgta tgactgggaa
     1141 aaattatacg cttcagatga atcggttatt tatgcaggat ttgctgcagc aaggaatatg
     1201 acgatcaagg tagaaggaat cgaagttgct tactcagatc ctgcgacaga tcctcctggg
     1261 caagaaagac ctactcgctt gattgatccg atttacactg tgacctctag taatcattca
     1321 ggaaaccaag atcatactat gtcctttaga gcaaatgctg acggattatt gacaattaaa
     1381 aataaagcaa caggagaggt gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg
     1441 gaatttgatt atcagacaaa actgatggaa ggaacgaacg aatttacttt cagttttacg
     1501 ccagataaaa actatccgcc agaagaatac gtagagatga ctagttatga aacaaaagag
     1561 attcttcata cagtggacta ccgtaaacct aaatattcga cagtttatgt aacaccagaa
     1621 gggtcagatg caggaaacgg aagcagacaa aatccattat ctctgacaca agcattgaga
     1681 tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa cttattcctt cgaaagagga
     1741 ttgacgattc caaaaggcgt taacgggaca agagaaaacc cgattcagtt gattgcagag
     1801 aaaaatccag aaaacaagcg tcctgtactt gattttaaag gaactggaaa tgggatgaca
     1861 ctgttcagtc actactgggg tttacgaggg tttgacatta caaatagtgc agctatgcaa
     1921 aaaggattac agatttctgg aaatcataat ttgatcgaag agattcatgc ttatcggaat
     1981 ggaaatacag gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg
     2041 cataacttag tcaagaactg tacatctttt gaaaatgccg atccaggatt tgaagatgcc
     2101 gatggatttg cggccaaact gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg
     2161 tatcataatg cagatgacgg ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga
     2221 aaagtaatca tcaaaaactc agttgcttat cgaaacggct gggtaccagg aattgaggga
     2281 gaaggtaacg gtaatggatt caaaatgggc ggttcttctt taacgggccc tcatgaattg
     2341 atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg attccaatag cggaacagat
     2401 attatcgtca atagcagtac aagttttaat aacggttcat ataatgtggc tctttatacc
     2461 tcttcagcag gaaacacaga ctaccatgct tctggtatct tatctttccg aacagagaat
     2521 cttgagatga gagagcagat ccgcccattg aatcaaaacg aagatcaaat ctaccactcg
     2581 ttgaactact attggaatga acgagagaaa caatcagaaa atactcttgg acataccata
     2641 tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag aaggtcagca gcctgttacg
     2701 cgctatgaaa atggaagtat ccatgttcat ggactgatgc agattaaacc ggggaatcgt
     2761 caaacagaaa aggaaagagt ggatggtctt ccattatctg taggtgctat ttttgacgga
     2821 gaaacctcac cgtcttcgga atatccagag tatgcaatcg tacaagaacc aaatcagaca
     2881 aaaccaaatg aacaaaataa tccgattatt atacaagaag caaatggtgt ggcggagaat
     2941 gcagaaggaa atatcattga actgaagcct tttacagttt ttgtgtcaac tttcacacca
     3001 aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt
     3061 gaaacacagt caagtgctca attttctaat cataccgcta ttttttcagc accttcaaaa
     3121 ggagaatata cgctagaagt cacttatcgt tcggaaatat ttgatgggga aaaatggtct
     3181 gctcatacag aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg
     3241 cccccgggaa atgacaaacc agaaagagaa aatccaggaa atgaatcacc aaatggaaaa
     3301 ccaaatggaa aaccttctca tccggatagg acagcaaaag aaagtgaaaa aaatggcact
     3361 caaggactcc caaaaacggg agaatttaat aacccgttgc tagcattagc tggaggaatt
     3421 ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc
     3481 cttttatgat gtaaaacaat tgactgagga aagtaaaaat agaaggaaat gagaccaata
     3541 gatgtctcac ttccttctat ctttttatag atgaggttct ccttgctgct tttgattggc
     3601 tgttcttatt acttgatgca ggacagcttt ttcacaatct ctccactaaa cggtgttcaa
     3661 tcagctgacc ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt
     3721 tttttctcta atactcaggt caggacgctg atttcacaac ctctcgataa acggtgttca
     3781 aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca
     3841 aaatttttct tactacttga aactaagcag ctttttcaca acctctaata catggtattc
     3901 cagaagtaac ttcttcgaaa aataagccga aatgtcacaa aaatttgaga agcgattttc
     3961 ccaaattcct tcttatttct tgaagctgac cacttctggc acaacctctt accagattcg
     4021 cactcttttt tctggcgcta gatacatagc gtcttgttcc gtgataccaa atgtttcata
     4081 gaattcgtca aggttttttg gttgcacgtt tgctcggagt ttagcagggg catggacatc
     4141 gatttgcagt aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa tcgttgccca
     4201 attgacaaag aattcctcga tagaaagatc tgattccgtt tttgctgctt ccaatgcaca
     4261 gcttagaccg cctgcatcag caatattttc cgaaacggtt aattttccgt ttacttttcc
     4321 gtcagcaaaa ggcagaccat cgaattcttc gatcattgct tgtgctttgt tttgaaattg
     4381 tgtaagatct tcttctgtcc accagttgtt caagttgcca tattcgtcaa ataaagcgcc
     4441 gttattatca aatgcatggg aaatctcatg agcgataaca gctccgattc caccataatt
     4501 agcagagctt gattgttcta gactgtaaaa tggtgcttga aggatcgctg ccggaaagac
     4561 gatcacattc cgaaatggat gataataagc attgaccgtg tcagcactca tttcccattc
     4621 tgaacgatcc actggtttgt tccatttgct gaaacgatct tttaatgtga gtttgctgaa
     4681 aacaagagta tttgaaagca aagtaccacc ctgatctgca ggtactgttt tgaactggtc
     4741 gaataatgct gggatcttat cggggtagcc tacttggata cctaagttat ttaattttat
     4801 gacggctttt tcttttgttg catccccaag ccagtcattt tcttctagac gttttttata
     4861 aacggcgatc atttttttca ccatttgttc tacgtctttt ttagcagctt cgccaaaata
     4921 tttttttccg tagtaatcgc caactacttg atcgaattgt ccagaggcaa ggtaatacgc
     4981 tgcttttttc tgaggcattg cttcgtctgt tccagacaat acgcgagaat agattccgct
     5041 gacttggcgg aattcttctg atagataacc actaagagaa cgtactgttt taatgatcat
     5101 ccagcttttg aataacggaa aatgagcttc tgtcaggatg tcattcaaat gatcgaagta
     5161 cactggatca gttacgatca ctttatctgg agttgtccca atcaaacccg tgacaagttt
     5221 tttcaagtcc agtttatctg tgtatttaat aaatgtatca aaagattgcg gattatacat
     5281 tttgctgtaa tctgcgtttt cttctgcact ttttacatga ggagcaatca atttatcaaa
     5341 ttgcactgct tgttccgtga ttgcttctgc ctgacttttt tctttgccgg caagctctaa
     5401 taactgaaca gccatatcaa agaaaacgcc tattagttgt gcaccgttgg gatggtctga
     5461 tgcataatac gtcttatctg gaaggaataa agaaggcgga taagcaaaga aggcgttcgt
     5521 ttgcgcattc ttcatatctg catctacatc cagattaaat ggcaatggta atgtatccaa
     5581 cagccattct ggaaattgct tattcaattc gtcgaaagac tgcaatgatt caactttttc
     5641 gattataggt tgaagcggtt gagcatgcaa ctcatttctt cggtcataat cgtttgccag
     5701 ttgataatat gcaaggaaat gtttcatttt ttctgaagac actttttctg gatgggcaag
     5761 cagatcgtct gtttctccca tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc
     5821 gcctgtagct ggtttatctg cagggatttt tgctgttttt tgccattctt cattgactgc
     5881 ttcatagaaa tcttgtttca atcgttcttt gttcattcat cgatcgctcc ttttttgttt
     5941 cgtgtgccca ttatcttaca aaatgaagga attaacaaat agaacataaa aaattgaaaa
     6001 ttcgaacaat caaactgatt tgtttttttc aatccttcac ttataatgaa aaaaaggagg
     6061 cgatcccaac tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt
     6121 aaaaaattag ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct
     6181 ctggtttttg aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat
     6241 tctccagcaa aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat
     6301 gaagagccaa tcgagttatc tagatggatc aacagaaaag aagatattcc cgttttgatc
     6361 gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt
     6421 tttttgaggt ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca
     6481 gcagttctcc ttgcctatta tcaagactat cgtaaggggt ggatgcttcc aaatgagatt
     6541 cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa
     6601 ccactaggac ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag
     6661 aagaatggca atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca
     6721 aaacgtatac gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca
     6781 tatggcaatc attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat
     6841 tacaaagtac acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac
     6901 ggaacagttt cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg
     6961 ttaaactatc gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga
     7021 tgacacgttg aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa
     7081 agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc
     7141 catcaatatc gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc
     7201 acctgtcaat gattactata tggaactact gatcatgatc gatgcaatga agcgagcaag
     7261 tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc
     7321 gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc
     7381 aactcgagta ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc
     7441 tgtagataat ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac
     7501 gggagatgac atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct
     7561 ttcagaatat ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga
     7621 gggtggttat gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt
     7681 gaacacagga gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga
     7741 agtatatgcc tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa
     7801 tgcaccaatc aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc
     7861 agaaaactta acaatcgtta catgttcaga attaatgggt gaagcggtca aacgcatcca
     7921 tgaaaataca ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa
     7981 aaaaagaggg aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt
     8041 ttgtcacagc tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc
     8101 tttggtttgt tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat
     8161 gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt
     8221 gaagcgatga cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt
     8281 ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa
     8341 gcaaaacctc acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc
     8401 ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa
     8461 catcctgctg ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat
     8521 ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa
     8581 gatacgatct tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc
     8641 aaagaaatcg gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa
     8701 gcgtacggaa accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct
     8761 gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat
     8821 atccctgcat tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat
     8881 ttagcaggga tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa
     8941 gcacgtaaca acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac
     9001 gaaatccctt atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt
     9061 cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct
     9121 atttctactg gttcagcttg tacagcagga aatatcgagc cttcacacgt attagaagca
     9181 atgtatggag aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga
     9241 aataccgaag acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa
     9301 ctagtgaagt cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa
     9361 cttaactgaa aaacctagta aaaatcagga taaactttta taataaagag aaactaattt
     9421 tatcgaggtg aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag
     9481 ctatcgtttg catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac
     9541 aaacggaggc aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg
     9601 attcaaatta aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac
     9661 agcaaatgga ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga
     9721 taaattttat tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg
     9781 atctgtggat ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt
     9841 gtaaggacga cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt
     9901 ttaatatttt gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat
     9961 ttagggaaaa aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct
    10021 atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac
    10081 gaattggaag agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag
    10141 tatggtcgtc caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat
    10201 tttgagtttt gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg
    10261 accgctagtg gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc
    10321 gattttcgcg cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct
    10381 agtttttgtg tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca
    10441 gtcgattatg ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg
    10501 aatggtccaa tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt
    10561 cgttcctgtc aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt
    10621 attgattttt gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat
    10681 ttttaaagat gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat
    10741 aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt
    10801 gatgacgcta gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta
    10861 cgcagtcgga atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg
    10921 aatgaaacct attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt
    10981 ggttccaaac gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg
    11041 tgcaacgcca tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa
    11101 tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg
    11161 attattattt gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc
    11221 cggaatcggg attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta
    11281 tgataaagaa ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta
    11341 tggaatggag ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt
    11401 ggcaataagc ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga
    11461 tatagggcag ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat
    11521 ttgttgctca gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg
    11581 tgcgtctacg cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc
    11641 atgaagattg cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata
    11701 ttgttgatgg tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc
    11761 gtcagtaagc ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt
    11821 ccctttgatc aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt
    11881 tgctttttca tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc
    11941 ttttattact gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa
    12001 gagccgcgga gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact
    12061 gataaagtaa ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta
    12121 tctgagcaac tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc
    12181 gataagaagc aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg
    12241 aaaggctggc cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt
    12301 gattttttgg ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta
    12361 cgacttgata attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa
    12421 aagttgcgac cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat
    12481 gacagacaac agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt
    12541 aacagcactt ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg
    12601 ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa
    12661 agtagctgct caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga
    12721 tcgtgtattt gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt
    12781 gatgtgcaac aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc
    12841 cgattatgta gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca
    12901 catgcttcgt ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca
    12961 agaacagctt tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc
    13021 aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt
    13081 tattggtgaa aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa
    13141 tatggtcact cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat
    13201 tggtcaacgt caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt
    13261 tgtaggaaaa gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc
    13321 attgtatgcc acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc
    13381 aaaagaattc aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt
    13441 tcgcttattg gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac
    13501 tcctggacaa gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga
    13561 ccacgcatat caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa
    13621 acagctatgg ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca
    13681 ataaaattaa tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc
    13741 caataatatg aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat
    13801 cttttgcaag tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg
    13861 acgggtatta gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt
    13921 gtgtttggga tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca
    13981 tggataatcg ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa
    14041 aagcgtccag atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta
    14101 ggtggcttca tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt
    14161 gcaacgatgc tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat
    14221 gttccttcta ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat
    14281 ggagttgaag gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta
    14341 ttcctatttt taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt
    14401 tggggtaacg tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg
    14461 cagattaaag gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt
    14521 gttttagcca accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg
    14581 ctttgtttat tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa
    14641 gaagagttag caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga
    14701 aattggggag cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg
    14761 tcttggacga tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg
    14821 aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca
    14881 atcttgacta atctattctt attgactttc ttagttactg attatgccta tcaatttgcc
    14941 tattctttat gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc
    15001 cagttttctt taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt
    15061 tttgaattga tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc
    15121 tatcttcctg gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg
    15181 ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg
    15241 cttgctgtag gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg
    15301 tgtagaagaa tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc
    15361 gatcgaagca ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt
    15421 tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt
    15481 caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa
    15541 tggagcgact ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt
    15601 tgtttcgatg cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc
    15661 tgttgacttt gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga
    15721 agccttagtc aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg
    15781 cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc
    15841 atatgtattg atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat
    15901 cgtagatgtc tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc
    15961 aggtattcgt attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta
    16021 tcgagactac acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt
    16081 gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat
    16141 tttaaaacaa tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc
    16201 tacttctttc attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt
    16261 agaagaaaca ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg
    16321 actaggttac tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt
    16381 cttgaaaaaa caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc
    16441 agtgtttttc atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac
    16501 tcctgaagct ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac
    16561 aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc gataaccaaa
    16621 tgcactggaa acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga
    16681 ttggttataa ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc
    16741 ttggacagca gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa
    16801 ttgccaaaca ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc
    16861 aatcgcaagt attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc
    16921 tatcaaagtg gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc
    16981 atgtccatgc agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga
    17041 tgcttggatg atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc
    17101 tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg
    17161 gacgaaaaac agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt
    17221 agggcgatag ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc
    17281 tgtaacagaa ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc
    17341 agttgcgaca agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg
    17401 caatgcgact aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat
    17461 tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca
    17521 ccaaggagag ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa
    17581 gttcaattgt gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat
    17641 gactaaacta aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg
    17701 ttcccaagtt tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc
    17761 ttctttctga aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca
    17821 atgaaagtct cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt
    17881 ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact
    17941 gctttcgaga actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca
    18001 aacgcttgga tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca
    18061 aaacagcgct aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg
    18121 tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt
    18181 tttcaaatca agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt
    18241 tattagatgg aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat
    18301 taacgaaaga agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc
    18361 tttccttacg ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct
    18421 atttttggat gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta
    18481 acaattttaa gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt
    18541 gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt
    18601 aattaccatg ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat
    18661 tgaagaagaa gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg
    18721 gaacgaaatc catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt
    18781 ttggaatgac ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa
    18841 gggacttgcg ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta
    18901 tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg
    18961 gtgctaatca aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc
    19021 ttggtcattt attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc
    19081 aaatggacac gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag
    19141 gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca
    19201 ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc
    19261 atttcaataa accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa
    19321 ctggattcaa gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg
    19381 cccgggcaaa agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt
    19441 acgcggataa tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc
    19501 aagaaaagaa aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg
    19561 gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag
    19621 cacaaaaact tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa
    19681 aattaaaaac ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca
    19741 atcaacgcta tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga
    19801 cccataagat cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa
    19861 gtacgatgaa agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt
    19921 tgaaaaacgg agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc
    19981 accatgcact tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc
    20041 gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag
    20101 tcagccaaac aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc
    20161 gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg
    20221 aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta
    20281 aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag
    20341 ctacgcgtgc agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc
    20401 aaggactgca agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag
    20461 taacaccaga tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga
    20521 aacaagcagg aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga
    20581 tcaaatcaag caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat
    20641 gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt
    20701 cagattgcaa ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat
    20761 gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg
    20821 atggaacgac agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg
    20881 aagaacgacg cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag
    20941 cagcagcact aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag
    21001 tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa
    21061 tttcggctta tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg
    21121 tgacaagact tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta
    21181 tattattggt gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa
    21241 acaggacgct gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga
    21301 cataacaatt ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat
    21361 gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg
    21421 acacaagaca aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg
    21481 ataccatcaa tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca
    21541 cgttcgcggt tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct
    21601 tttgaaaatc ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc
    21661 aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg
    21721 tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg
    21781 gtgccgacac aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg
    21841 ggcggatctt gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat
    21901 ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa
    21961 ggataagctg aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca
    22021 ataaacaaat attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga
    22081 ggaacagttt tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc
    22141 ctttcaatcg tatgtttata tatggtttac attcaaaatt taataaacct taaataacac
    22201 ctttaaaatt tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag
    22261 tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa tttttattag ttggcaagta
    22321 gttttggata gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa
    22381 aatacacaca cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt
    22441 atcttttcag ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca
    22501 agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat
    22561 aaagagaggt acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg
    22621 agaaatccga acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc
    22681 gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat
    22741 cgggcaaaat ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa
    22801 acgccaatt
//
LOCUS       DAYYFD010000047        22758 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000047 DAYYFD010000000
VERSION     DAYYFD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.047"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHA25_001659"
     CDS             complement(132..509)
                     /locus_tag="ACHA25_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762952.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHA25_001660"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHA25_001660"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8762953.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHA25_001661"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHA25_001661"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8762954.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA25_001662"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA25_001662"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8762955.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHA25_001663"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHA25_001663"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8762956.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHA25_001664"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHA25_001664"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8762957.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHA25_001665"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHA25_001665"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8762958.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHA25_001666"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHA25_001666"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8762959.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHA25_001667"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHA25_001667"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8762960.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHA25_001668"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHA25_001668"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8762961.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHA25_001669"
     CDS             10661..11575
                     /locus_tag="ACHA25_001669"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8762962.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHA25_001670"
     CDS             complement(11708..13273)
                     /locus_tag="ACHA25_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762963.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHA25_001671"
     CDS             complement(13266..14717)
                     /locus_tag="ACHA25_001671"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8762964.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHA25_001672"
     CDS             complement(14731..15747)
                     /locus_tag="ACHA25_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8762965.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHA25_001673"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHA25_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8762966.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHA25_001674"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHA25_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8762967.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHA25_001675"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHA25_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8762968.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHA25_001676"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHA25_001676"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8762969.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..>22758
                     /locus_tag="ACHA25_001677"
     CDS             21340..>22758
                     /locus_tag="ACHA25_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8762970.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLT"
BASE COUNT         7328 a         3840 c         5155 g         6435 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaaca
//
LOCUS       DAYYFD010000048        22637 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000048 DAYYFD010000000
VERSION     DAYYFD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.048"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHA25_001678"
     CDS             complement(227..1114)
                     /locus_tag="ACHA25_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762971.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHA25_001679"
     CDS             complement(1111..1980)
                     /locus_tag="ACHA25_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8762972.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHA25_001680"
     CDS             complement(2117..3403)
                     /locus_tag="ACHA25_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8762973.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHA25_001681"
     CDS             3676..4254
                     /locus_tag="ACHA25_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8762974.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHA25_001682"
     CDS             4254..5183
                     /locus_tag="ACHA25_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762975.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHA25_001683"
     CDS             5180..6916
                     /locus_tag="ACHA25_001683"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8762976.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHA25_001684"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHA25_001684"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8762977.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHA25_001685"
     CDS             complement(7812..8321)
                     /locus_tag="ACHA25_001685"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8762978.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHA25_001686"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHA25_001686"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8762979.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHA25_001687"
     CDS             complement(9622..10713)
                     /locus_tag="ACHA25_001687"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8762980.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHA25_001688"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHA25_001688"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8762981.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSGDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHA25_001689"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHA25_001689"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8762982.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHA25_001690"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHA25_001690"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8762983.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="ACHA25_001691"
     CDS             complement(14676..15650)
                     /locus_tag="ACHA25_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8762984.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="ACHA25_001692"
     CDS             complement(15631..17514)
                     /locus_tag="ACHA25_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8762985.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="ACHA25_001693"
     CDS             complement(17532..19757)
                     /locus_tag="ACHA25_001693"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8762986.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="ACHA25_001694"
     CDS             19872..20906
                     /locus_tag="ACHA25_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8762987.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="ACHA25_001695"
     CDS             complement(20973..21986)
                     /locus_tag="ACHA25_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8762988.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="ACHA25_001696"
     CDS             complement(22064..22252)
                     /locus_tag="ACHA25_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8762989.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6448 a         4864 c         4096 g         7229 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcacc actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aactaaa
//
LOCUS       DAYYFD010000049        22165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000049 DAYYFD010000000
VERSION     DAYYFD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.049"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACHA25_001697"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACHA25_001697"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8762990.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACHA25_001698"
     CDS             2370..3848
                     /locus_tag="ACHA25_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8762991.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACHA25_001699"
     CDS             3814..5145
                     /locus_tag="ACHA25_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8762992.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACHA25_001700"
     CDS             5259..7811
                     /locus_tag="ACHA25_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8762993.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACHA25_001701"
     CDS             complement(7818..9257)
                     /locus_tag="ACHA25_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8762994.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACHA25_001702"
     CDS             9232..9414
                     /locus_tag="ACHA25_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8762995.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACHA25_001703"
     CDS             9603..9965
                     /locus_tag="ACHA25_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8762996.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACHA25_001704"
     CDS             complement(10152..11009)
                     /locus_tag="ACHA25_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8762997.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACHA25_001705"
     CDS             11129..12040
                     /locus_tag="ACHA25_001705"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8762998.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13698
                     /gene="mdcA"
                     /locus_tag="ACHA25_001706"
                     /pseudo
     CDS             12047..13698
                     /gene="mdcA"
                     /locus_tag="ACHA25_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13800..14099
                     /locus_tag="ACHA25_001707"
     CDS             13800..14099
                     /locus_tag="ACHA25_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8762999.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14089..15765
                     /gene="mdcD"
                     /locus_tag="ACHA25_001708"
     CDS             14089..15765
                     /gene="mdcD"
                     /locus_tag="ACHA25_001708"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8763000.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15769..16362
                     /locus_tag="ACHA25_001709"
     CDS             15769..16362
                     /locus_tag="ACHA25_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8763001.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16386..17462
                     /locus_tag="ACHA25_001710"
     CDS             16386..17462
                     /locus_tag="ACHA25_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8763002.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17487..18356
                     /gene="mdcB"
                     /locus_tag="ACHA25_001711"
     CDS             17487..18356
                     /gene="mdcB"
                     /locus_tag="ACHA25_001711"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8763003.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18467..19231
                     /locus_tag="ACHA25_001712"
     CDS             18467..19231
                     /locus_tag="ACHA25_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8763004.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19720..21813
                     /locus_tag="ACHA25_001713"
     CDS             19720..21813
                     /locus_tag="ACHA25_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8763005.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21838..22140
                     /locus_tag="ACHA25_001714"
     CDS             21838..22140
                     /locus_tag="ACHA25_001714"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8763006.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3787 c         4722 g         6477 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaatgca
    13381 ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg aattgcttat
    13441 ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat tggcggtgtt
    13501 acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc agccggcatc
    13561 gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc attattagct
    13621 gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc accaatgaaa
    13681 ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta cccagatgga
    13741 agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg gagagatcca
    13801 tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa atccatgtag
    13861 gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa acagaatcta
    13921 ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat gtattaacga
    13981 gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc ggtgctacac
    14041 caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat gaaaaatagt
    14101 tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg acgtgaattg
    14161 attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat cgtccctgaa
    14221 agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc agtagtaatc
    14281 gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc aaaaatcgtt
    14341 gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt tccagtcatt
    14401 gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt atctatttca
    14461 gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg tttgattcca
    14521 ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag ttatttaatc
    14581 gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca agaagcaggc
    14641 gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg atctcgtcaa
    14701 cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc aatcaaagct
    14761 gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag ctcagcattt
    14821 tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga cgattacaat
    14881 gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc agaaggaaat
    14941 gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg catgaaaaat
    15001 ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat agaagaagcc
    15061 atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg aaacggtgaa
    15121 gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta cgaagaagat
    15181 aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag ccaagcatac
    15241 ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc tgctgcttat
    15301 gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa tgctatttca
    15361 ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa caataaagaa
    15421 attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg ttcggttgct
    15481 gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa taattataca
    15541 aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc ttcagaacaa
    15601 aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg caatacaaat
    15661 gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg acgtgtcgaa
    15721 acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat gattcaagca
    15781 catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc tgaatgggtc
    15841 gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca cgcagaaata
    15901 ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga aatctcaaaa
    15961 catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc gtatagacaa
    16021 gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa catattatca
    16081 ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg gattccggca
    16141 gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat tgaattgcca
    16201 attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca agtagtgact
    16261 cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa aattctattg
    16321 aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa aagagagaga
    16381 aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag aaagtatcag
    16441 ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac agccacagcg
    16501 caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca gcaccaatgt
    16561 ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct tattgggaat
    16621 ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg acggcttatc
    16681 tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca agctttttac
    16741 tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg atgctgcaga
    16801 tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct aataaaaaat
    16861 atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat ctgttctatc
    16921 aaacacaagt attgactggg ccttatcatg acagctggta cttattagat cggtttgttg
    16981 ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc tatcaatatt
    17041 tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc ttttctgtcc
    17101 gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac ttgaatacga
    17161 tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct tccaaaatga
    17221 ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat gcttatcgta
    17281 cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa gatagttggc
    17341 tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca agttgtgcat
    17401 ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt ttcagcaagt
    17461 aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa gcagtgactc
    17521 agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta agtccaaaac
    17581 ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat gctttatttc
    17641 ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata gcatggcatc
    17701 atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa gcggaaaagg
    17761 caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg gcaatggggc
    17821 tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct ttatcgaaaa
    17881 tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt cataaaaaaa
    17941 ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat gaagaagcga
    18001 tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta gataatgatt
    18061 catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta gatgacacat
    18121 gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt gctcatgctg
    18181 ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt tgtcaggaaa
    18241 accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc atgctgaaga
    18301 tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat gactaaataa
    18361 gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct tttagcaagg
    18421 ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga aaaaaatcaa
    18481 ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga cccaaggatt
    18541 cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg gcagaggttc
    18601 atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg gcagagattc
    18661 ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg gatttcttgc
    18721 attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga ataaaagaca
    18781 aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta aagaaaagaa
    18841 agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt gggaagaaga
    18901 aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac atcagaaagt
    18961 tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa ctattcagga
    19021 agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg atttcagatg
    19081 ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta agacacaacg
    19141 acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa gatggcaagc
    19201 cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg gagttgtgac
    19261 aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata gcctatcggc
    19321 aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt cttatcgctc
    19381 aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc ttgcatctgc
    19441 gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt tgttcttatt
    19501 acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca aaatttttgt
    19561 aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct tattttttgt
    19621 ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt tattatgtaa
    19681 gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag aacaattgaa
    19741 atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct agcaagtgag
    19801 ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat ctggttgagc
    19861 agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt gaagctggaa
    19921 aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg ctttttgaat
    19981 cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat cccgatccat
    20041 ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga agatatgaga
    20101 cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg aaagcaagga
    20161 ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt tatcaatcag
    20221 aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc ggctcaaaaa
    20281 atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat ctattccaat
    20341 aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt tacattgatc
    20401 tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa aaatacagaa
    20461 ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt tcaattggac
    20521 aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt caatgctaga
    20581 gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca attgatccaa
    20641 tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt attcgaaaat
    20701 ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca agtcatgaat
    20761 ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt cactcatttt
    20821 gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat agcttttctt
    20881 gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta tgtttataaa
    20941 tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga gaatctgaaa
    21001 gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat cgaattagta
    21061 gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg aaataagccg
    21121 cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga agactttttg
    21181 aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac ggagttattc
    21241 actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat catctatggc
    21301 aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga gatccagcct
    21361 atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc cactacttgg
    21421 gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt cgaagagcgc
    21481 tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt gattggcaaa
    21541 cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg agtcagcgta
    21601 gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt aaagattatt
    21661 ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga actaattgaa
    21721 ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat cgaagatttc
    21781 aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag aacaaagatg
    21841 aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt tgcagcacag
    21901 atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga ccattgtgat
    21961 atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa ttttcgttcg
    22021 cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa aaatattgtt
    22081 tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg agttatctaa
    22141 atacaaaaag aaaagagggt aaaaa
//
LOCUS       DAYYFD010000050        21813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000050 DAYYFD010000000
VERSION     DAYYFD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.050"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHA25_001715"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACHA25_001715"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8763007.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHA25_001716"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACHA25_001716"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8763008.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHA25_001717"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACHA25_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8763009.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHA25_001718"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACHA25_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8763010.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHA25_001719"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACHA25_001719"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8763011.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHA25_001720"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACHA25_001720"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8763012.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACHA25_001721"
     CDS             complement(8557..9027)
                     /locus_tag="ACHA25_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8763013.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACHA25_001722"
     CDS             complement(9073..9498)
                     /locus_tag="ACHA25_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8763014.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACHA25_001723"
     CDS             complement(9565..9834)
                     /locus_tag="ACHA25_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8763015.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACHA25_001724"
     CDS             complement(9845..11440)
                     /locus_tag="ACHA25_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8763016.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACHA25_001725"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACHA25_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8763017.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACHA25_001726"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACHA25_001726"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8763018.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACHA25_001727"
     CDS             15906..16880
                     /locus_tag="ACHA25_001727"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8763019.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACHA25_001728"
     CDS             17050..18450
                     /locus_tag="ACHA25_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8763020.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACHA25_001729"
     CDS             complement(18528..19250)
                     /locus_tag="ACHA25_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8763021.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACHA25_001730"
     CDS             complement(19329..19835)
                     /locus_tag="ACHA25_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8763022.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACHA25_001731"
     CDS             19958..21757
                     /locus_tag="ACHA25_001731"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8763023.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6053 a         4752 c         3911 g         7097 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ttgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttg
//
LOCUS       DAYYFD010000051        20994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000051 DAYYFD010000000
VERSION     DAYYFD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.051"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACHA25_001732"
     CDS             185..1183
                     /locus_tag="ACHA25_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8763024.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACHA25_001733"
     CDS             1295..1804
                     /locus_tag="ACHA25_001733"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8763025.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACHA25_001734"
     CDS             complement(1834..2493)
                     /locus_tag="ACHA25_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8763026.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACHA25_001735"
     CDS             complement(2495..3259)
                     /locus_tag="ACHA25_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8763027.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACHA25_001736"
     CDS             complement(3267..3944)
                     /locus_tag="ACHA25_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8763028.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACHA25_001737"
     CDS             complement(3981..5366)
                     /locus_tag="ACHA25_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8763029.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACHA25_001738"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA25_001738"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHA25_001739"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHA25_001739"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8763030.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="ACHA25_001740"
     CDS             6304..7161
                     /locus_tag="ACHA25_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763031.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="ACHA25_001741"
     CDS             complement(7242..7577)
                     /locus_tag="ACHA25_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763032.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="ACHA25_001742"
     CDS             complement(7644..9014)
                     /locus_tag="ACHA25_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8763033.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="ACHA25_001743"
     CDS             complement(9092..9628)
                     /locus_tag="ACHA25_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8763034.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9937..11418
                     /locus_tag="ACHA25_001744"
     CDS             9937..11418
                     /locus_tag="ACHA25_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763035.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11521..12228)
                     /locus_tag="ACHA25_001745"
     CDS             complement(11521..12228)
                     /locus_tag="ACHA25_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8763036.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12307..12645)
                     /locus_tag="ACHA25_001746"
     CDS             complement(12307..12645)
                     /locus_tag="ACHA25_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8763037.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12756..13718)
                     /locus_tag="ACHA25_001747"
     CDS             complement(12756..13718)
                     /locus_tag="ACHA25_001747"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8763038.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13733..15064)
                     /locus_tag="ACHA25_001748"
     CDS             complement(13733..15064)
                     /locus_tag="ACHA25_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8763039.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15072..15752)
                     /locus_tag="ACHA25_001749"
     CDS             complement(15072..15752)
                     /locus_tag="ACHA25_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8763040.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15971..16450)
                     /locus_tag="ACHA25_001750"
     CDS             complement(15971..16450)
                     /locus_tag="ACHA25_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8763041.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16577..18256)
                     /locus_tag="ACHA25_001751"
     CDS             complement(16577..18256)
                     /locus_tag="ACHA25_001751"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8763042.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18804..20306
                     /locus_tag="ACHA25_001752"
     CDS             18804..20306
                     /locus_tag="ACHA25_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8763043.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20319..20981
                     /locus_tag="ACHA25_001753"
     CDS             20319..20981
                     /locus_tag="ACHA25_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763044.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
BASE COUNT         6262 a         4353 c         3574 g         6805 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgata tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agacaactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcactatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
     9661 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
     9721 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
     9781 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
     9841 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
     9901 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
     9961 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    10021 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    10081 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    10141 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    10201 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    10261 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    10321 acaaaacagc tcaatcattc cttaataaaa ttccatttac ggatcctatc tgtaaaaaat
    10381 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    10441 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    10501 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    10561 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    10621 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    10681 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    10741 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    10801 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    10861 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    10921 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    10981 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    11041 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    11101 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    11161 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    11221 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    11281 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    11341 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    11401 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tattccaaat aaatggtatt
    11461 caatcagcta actctcggta ttagctcaga tcaaagcgct gatttcacaa cttctttatt
    11521 ttaatctctt cggttgccgc cgaacaataa gatcaagcgc aacagctgca ggaatgtcga
    11581 aagcgctgct gcaacataag tcagtgctgc tgcaaataat acgtgtcttg ccatcggtac
    11641 ttcttcttcc gtcagtagtc cgccttcact caaaatttgg attgctcgcc gagacgcgtt
    11701 aaattcgact ggtaatgtca ccaattggaa aagtaatgct aaagaaaaag cgaaaatccc
    11761 gatattgatt aacgtagagt tccagctaag caatacacct accataatca aggggaacga
    11821 aatcatcgaa ccaaagttag ctgctggaac aagtgctgca cgaatcttca atgggacata
    11881 acccgaagca tcttgtaccg catgtccgca ttcatgagct gctacaccta tcgcagcaac
    11941 tgaagtagac tgtgcagttg cttgagacaa actcaacatc ttattacctg aattgtagtt
    12001 atctgtgaga tctccggcaa tttgttgaac accgacatta tttatctgtt gactttgtaa
    12061 tatatattgt gctgcttgtg taccagtcac attgtttttg cttctcactt gatcgtattt
    12121 tcggaaagta ctattgacgt acgctgacgc aattccggaa ataactaatc caagaatgac
    12181 caaaagaaat gtcggatcta aaaaaccata cattccataa ccatacattt tcgtttcact
    12241 ccttttcgtt tactttattt tatcattagt agtaggaaaa gaatgtgaaa agaccttgaa
    12301 tattctttaa ttctttttca caaattccga tttcagtttc atcggtccaa atccttctac
    12361 cttgcaatcg atattatgat cgccttctac taaacggatg ttcttgactt ttgttccttg
    12421 tttaatggca cctgacgcac cttttacttt caagtcttta atcactgtca cgctatcgcc
    12481 ctctaacaga cggtttccat ttgcatctaa taccactaat ccgtcttcta tgacagtttc
    12541 ttgttcttct ttgttccatt cgttcccgca ttctgggcaa atcaacaagc ctcgatcttc
    12601 atatgtgtac tctgagccac attctgggca atttggtaat gtcatcttct ttctccttta
    12661 aaatctttag tgagcactat tgtaacggaa aaagaagatc ttgaaaagat acatttgtga
    12721 tttttactac aaaaggcacg ttttcgaacg aagtatcatt ctgtttttct gctcttatac
    12781 gattggcata tatcttggat ctctttatag atttcctcta cttctgctaa atcttttgca
    12841 ttcgcaccac ttgctaatgg atggcctcct ccatgatggc gtttagccaa ttcattgatg
    12901 atcggcccct tagaacgtaa acgtacgcgg taataccctg aaggctgctg aacaaaaatc
    12961 ccccaagcca gcacttcgtc aatcactcca ggaagtgaaa cgatcgaagc tgtctctgaa
    13021 tcatcgattc catatttatc aagaatttct tgcggaagga ttattcttcc tgcaccattt
    13081 tcatccacct ggatattttg ataaagataa ccggaaagtt tagcgacttt catcggcatt
    13141 tgttcaatct ctctgttcaa agcacttgca tcaaatggat aatttctcaa tatcgaagca
    13201 atctctaatg tacgggacgt cgtggaggga tacaaaaagc gccctgtatc tccgacgatt
    13261 cctgcataaa gtaaccgtgc agccttaacg ttcatcttta aagattcagg aaacatcaaa
    13321 gcaaattctg caatcatctc actacagcta cttgcttttg tatttaccca cacaagatct
    13381 ccataagggt catcattggg atgatggtcg attttgatca gtttttctgc caactgatat
    13441 ctttgatcac tgattctggg tgaattagct gtatcagtca cgatgactag cgctccttgg
    13501 tagaagacat cttcaacttc ttgcatttct gctaaaaagc gaagcccttc aatcgtttca
    13561 cctacttgac gtatttcttt ttctgggaaa ctttcccgta atagttcggc taatcctact
    13621 tgtgatccta atgcatcagg atcagggcgc aaatgcctgt ggataatgat tttatcgtat
    13681 ttttttattg tatctactat ttcttgctgt actgtcattt cactcctgct ttctaagaac
    13741 gttccattac ttgacaaaca acgattgctt tggaaacgat ggtgttttca ataaacactt
    13801 cgatatccag tttagcggaa cgtctgccga tttccaatac acgtggacga atgtctaatt
    13861 cgctttccaa ttggatcagc cgcaaataat gtaggttcac ttgttcaatc aagatatttc
    13921 gtttttggtt catcaccatt gttttttgtg cgacatcaga aatgatttcg ctaagtacgc
    13981 caaaagaaat ggttccaacg ccattgacca tttgtggtgc tactacgaat ttgaacttag
    14041 ggttttccaa acgatttcct tcacggtcag tatcaatcgt atggatattt ccagatatct
    14101 gatccgaaat cgtatctgca atctgcggtt gtcgctggac aagctgcatc gctttcatta
    14161 catcttgccg ggtaatcaag ccttccaatg tcaagtcatc ggaaacgacc ggcatgactt
    14221 ctagcccatc ccagatcatt tgatggctaa cagaggcaat actcattgtt ttctttacac
    14281 tgattggttc tttcgtcatc acccgatcga tgatctggtt tggcgatttc cctaaaacat
    14341 cttttgccgt aacaatcccc atcaaacgat gatggcggtt gacgactgga aaacgcgaat
    14401 gctgcgtttc ttctgacaaa cgctgataat cagaaatcgt atctgtctgc tgcaaataat
    14461 gagtcttttc agctggcata tagatatcac tgacaagcat gatatctttt ttgatcaatt
    14521 ggtcgcttaa cgctcggttg atcatggttg cgactgtaaa tgtatcataa gaggtgcgta
    14581 agatcggcat ttcaagatca tcagctagct ttgcaatctc agcagtcgta ttgaatcctc
    14641 ctgtgatcaa tactgcggcg ccatcttcca atgctaattt ttggacttcc gcccggttgc
    14701 cgacgatcat tagtgatcct ggcgtaatat agcgttccat ggcatgttta gtcatcgcgc
    14761 cgatgacgaa tttattcaag accttgtcta atcctgccgc cccgcctaaa acgtcccctt
    14821 caatgatcct gacgacctcg ccaaatgtca aacgttcaat atgttttttt aatttgcgtt
    14881 cgatacggat ggtccctact cgttgaattg ttgaaactag tccgacattt tctgcatctt
    14941 tgattgcacg gtacgctgtt ccttcactga cattcaattc ttttgcgatg cttcttacag
    15001 aaattcgatt gccaacaggt aaactttcaa tatattctaa aatcaaatca tgttttgtcg
    15061 ccattttctg cctacacttc cagcgtttcc gagacgctca ttatttttcc ttcactatct
    15121 tccagtaact ctacaaaaga ttcgggatct tgttcaatga cagggaaagt attgtaatgg
    15181 attggaacta ctttcttggc acgaattgtt tgcgcagcct ttgctgcatc ttctggcccc
    15241 atcgtatagt tatcgccaat cggcaagaaa gcgacatcaa tatcgaattc ttctgcaagc
    15301 aatcctagat cactgaaata agcagtatcc cctgcatgat aaatcgtttt tccttctgct
    15361 tgaataataa ttccgctagg ctcgcccata tactgaacga taccgtctac ttcatagctt
    15421 gaactatgct gtgcatgtac gaattttacc acgccgaatg gaaacgcatg ttttccaccg
    15481 atattcatac cgaatgaatc tactcctctg ctgttcgcat aatcagcaat ctcaacaata
    15541 ctgataattg tggcttgatt ttcttttgca ataggaatca tatctcctat gtggtcacta
    15601 tgaccatgag taatcagcaa ccaatctgtc tttacttctt ctgcagttaa gtctgtcaat
    15661 ggatttcctg taataaacgg gtcaatcatc acgcgttggc cattttccat ttcaattgtt
    15721 aaacaggaat gtccatgata cgtaattttc atattttaca cctcgcgttt tctctgtatc
    15781 ttttttatta aaaacgattt catcacttgc acatcaatga aaaaatatag ctgctaaata
    15841 tattttcatt ggattgatag tacagtatgt acaaagcaat tgtaacagat aatacaggat
    15901 gcaccaagta ccaagcagat ataatctcaa gtttgaggtc tctgctctgc ttagcttctc
    15961 ttctcgtccc ttaactaatt aaatcagcgt aaataggatg gacgatcttt tctaaatcgg
    16021 ctacttttat ccgaaggctt ctcccgattt caccgcttga cacaattact tcttcttttt
    16081 cttttaaaga actgtctgct aaaaaacaat aatcaggatg tgcgagtttt attccaatag
    16141 gggtgtttgc tccgtgggga tatcctgtat gcattaatac aaaatccatt ccaggaaaac
    16201 caactttacg atctttcgtc acttgtctcg tttttttgta atctaagtga tcgttcaaag
    16261 gaatcaatgc gatgatcacc ccagttttgt ttcctttcac tacgagagtt ttacatatca
    16321 aagctggatc aatccctctt ttttgtagtt cttctgtgaa agattcttct accacatgac
    16381 taaaatcaag tggttgatag ctgatctttt gttcttctaa ataacgttcg acttttgttt
    16441 gactcatcat tgattcactc cctttattct atcgtttatt ataaagcgag ggatgctgta
    16501 ttcaaaaatt atttgttttt cgcaaaaaaa gagcaagacg agcactctat agagaggttc
    16561 atcttactct ttttgtttat tttttatttc gacgttgagt agcttccata atgactggca
    16621 agatcactgg gcggcgtttt gtttgttcaa ataagtaacg gcctaattga tctcggatgt
    16681 cttgctttaa tttgctccac tcaaaatcat tgctttctaa atgtttctca acgattgttt
    16741 caatcatatt gctgctttct ttcatcagat cacggctagc ttttacgtaa acgaatcccc
    16801 gtgaagtaat ttgaggtttt gaaatgattt tcttttctcg ccgactgata gtgacaaccg
    16861 ctacgaaaat cccgtcttct gacaagattt ttcgatcacg taaaacaatg tttccgatat
    16921 cacctatgcc taatccatca atcattacat tttctgcact tgtagttcct gcaacactca
    16981 tacgcttgtt cttatattcc agcacgtctc cgcgtccagt aatatagata tttttgtatg
    17041 gcatccctac tgcatgagcc aaatctgcat gagcagctaa ttcacgatac tctccttgaa
    17101 ctggcacaaa atatgttggt ttcatcaaat tgatcattaa ttgcaagtca ttaggattgg
    17161 catggccaga aacgcgcata ttctcagaga tcaacttcac tgttccacca gctcgataaa
    17221 tgatatcttc cgttttcgcc acgatggttt ccatcgcgat gcttggcgta gtagtgatat
    17281 aaaccaaatc tccttcttca atcttgacat tacggtgtgt accgtttgcc attttttgca
    17341 gagacttgat gggttcaccc attcggccag tttctaaaat gatcagttct tctggatcat
    17401 agtttttcat gtctttcaac gtaacaagca gatcttcact tggtaattga agtttttcta
    17461 aacgcatcgc tgtgcgaata attcgttcga aatcttgacc agtcaagaca acttttcgtt
    17521 gtgctttttc tgctgcatcc aatacttgtt gcactcgttg caaattactt gctacacaag
    17581 cgacgatgat acgcccttcc caatatttga tcgtatcaaa tacttcatct gctatttggg
    17641 cttcagaagc tacaggctga ggattttctg catttgccga ttcacttagt aaggctaaga
    17701 ctccttcttt gccgatttcc gccaatcgtg cataatctgt ttgatacatt gggatagccg
    17761 tttggtcaaa tttaaaatct cccgtataaa cgatactacc ttcttttgtt ttcagattga
    17821 tccccactga atctggtatc gtatgagtgg tacggaaaaa gctcactact gtttcaccaa
    17881 agtctatttc tgtatgctca tctaccacat ggaaatcctt aaatcctttg gctgcttcat
    17941 tacgactgac attcaatttt gctaactcga tcgtcaactc tgtcccgaat actggtacag
    18001 aaatttttga caacaagtaa ggaagtgccc cgatcgcatc tgcatgtcca tgtgttaaaa
    18061 agacaccagc tacacgatca atgttttctt ctaaatacgt aaaatcaggg attacgacgt
    18121 cgatccccaa caactcattt tcaggatatt ttagcccaca atctaaaata aaaatatcct
    18181 cgcccacttc tgcgacatac atgttttttc cattttcacg aacgccgcct aatgggataa
    18241 ttttaatggt actcacaaac ttcacctctt cttatgacat ccaagtttaa agatgtcgat
    18301 tttcttcttt atctaaatat atgagccatt ccaatgaagc atatgcaaaa tcactatttc
    18361 tcaacttatg gagaatcagc aggaaaatta ccagcacatc cgcgttctga cagttggatg
    18421 ggcattttat tttggatata cttcattaac aaaatggatc ttttgcgaaa cgctatagcc
    18481 agaatactta tataagtatt cagctgttcc ccattattat acaacaaata aactaaatcg
    18541 ctagtatcag caattgttag aacaactatg cacgtaaaaa tccctctgta gcatctagga
    18601 tttcttttgc tattcctctt tcatttcttc ttcgtcggtt tgaaacatac ttttttcatt
    18661 ttaccacatt ttttgcaata actatagtga ctttttatat atacagaacc tcatgttgat
    18721 ggtgtgcaaa cagcataaga tttaagctag ctttaagata ggttgtctat tatcaaatgc
    18781 atctactagg agggatgcaa aatatgaatt ttatgaaacg tgcatggaaa agtacaaaag
    18841 taaaatgggg acgttcattg ttattgtttg ccgtgttcac agccattctt atttttgtgc
    18901 tagctggcct cacgattcga agcgctgcag aagaagcagc gaatcaagca caaaaagaag
    18961 ttggagcaac agtgacttta agtgccaatc gagaagcttc atttaaaaaa caggaacaaa
    19021 cttcttcaag cggttctacc actgaagaac gtccagatcc aggcagtttt tctcttacac
    19081 cagtttcctt aacggacgcg gaaaaaatag ctgaattaga taatgtaaag agttattcct
    19141 ttgaaagttc cgcctcagct atggcaaaag atggaataac tgccatcact agttctgatt
    19201 caagtgaaga cagttcagaa actactgatg caggcggtga acctgctgga ggaatgggcg
    19261 ggaatccgca aatgactcaa gcagactttc aaatcagtgg tgtgtcagca actgcacaaa
    19321 atagtaattt ttctgatgga acagcaaaaa tcacggatgg tgaaggaata gatgacagcg
    19381 acaaaggcac aaacaatgta gtcattgatt ctactctagc agaaacaaat gacctggcag
    19441 tcggagatac tttcacaatc acgagtactg aagatgaaga taccacttat gaaatgacga
    19501 tcaaaggaat ttatgaatcc agcgaaacaa gcggcagtat gggtatgaaa ttcaacttca
    19561 tgaatccgtc gaatacactt tacacttctt acacttttgc aaatgaacta aatgggacat
    19621 ctgacgataa cacgatagat tctgctattt acactctatc tgatccagat aaaatggatg
    19681 agtttctcaa agaagctgag aagctgatcg acacagatac atttagctta caatcaaatg
    19741 attccatgta tcagtcaatg ttggaacctt taaacaacgt ggcaagtttt tctaaaaatg
    19801 ttgttttact agtggctgta gccggtatta tcatacttac tctgatcatt atgattacca
    19861 ttcgggagcg tcggcatgag ttaggcgtct tgctatcttt aggagaatcc agaagtaaaa
    19921 tcattctcca attgtttacc gaagtggcta tttgtatgat tcttgcatta ggagttgctt
    19981 cgcttagcgg aaacgtcgtc gcgaatgccg tgggacagca gctacttgac caacagacag
    20041 aaaccacgac acaaaaccag caagctcctg gcggtggaca aatgccaggt aacagacaag
    20101 aacaaggcgg tcaacgcgat ggtcaacaag gaaccacgaa taatccattt gctgtttctg
    20161 atcaagtcag cgaattggaa atcaatgtca aacctgcaca attgggacta ttagccggag
    20221 tagcatttgg aatcagtctg ttttctgtct ttttagcttc tatagggata ttaagattaa
    20281 acccaagaaa aatcctatta aactaaagga ggaatttgat gttacggaca gaacaattgg
    20341 gattttggta tcaagatccg caagatgcgc tttataaaga tgtagaactg aatttcgaag
    20401 ctggaaaaat gtacgctatt ttaggagcaa gtggttctgg aaaaacgact tttctttcgc
    20461 ttatttctgg actagatgca ccaaaagaag gaaaggtata ctatcaagat cgttcactga
    20521 acaaaatcgg gttaagaagt tatcgtaaaa atgatgtctc gattattttc caagcttaca
    20581 acttgcttcc ttatttatcg gctctagaca acgtcatagc agcaatggaa atcactaaga
    20641 ccaagcaaga gaattatcga caagttgctt tagatcgact ggctcgtgta ggaattgatg
    20701 aaatattagc aaaaaaacag gtgaccaaac tttcgggagg tcagcagcag cgagtcgcta
    20761 tcgttcgtgc tatctgctgt gaacatcaac taatcgtagc ggatgagcct actggaaatc
    20821 tagatgaaac aacttcacaa gatatcgtca aacttttcca agaaatcgcc catgaacaaa
    20881 atcgctgtat tatcatcgtg actcatgaga ttgaagtagc aaaaagctgt gatgaagtct
    20941 ttgaactaaa aaataaaaaa tttcaaaaaa tcgaagttta aaaaacagga taat
//
LOCUS       DAYYFD010000052        20578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000052 DAYYFD010000000
VERSION     DAYYFD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.052"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..1240
                     /locus_tag="ACHA25_001754"
     CDS             38..1240
                     /locus_tag="ACHA25_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8763045.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(1417..2685)
                     /locus_tag="ACHA25_001755"
     CDS             complement(1417..2685)
                     /locus_tag="ACHA25_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8763046.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     regulatory      complement(2736..2803)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(2846..3493)
                     /locus_tag="ACHA25_001756"
     CDS             complement(2846..3493)
                     /locus_tag="ACHA25_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8763047.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(3758..4261)
                     /locus_tag="ACHA25_001757"
     CDS             complement(3758..4261)
                     /locus_tag="ACHA25_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8763048.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4385..5149)
                     /locus_tag="ACHA25_001758"
     CDS             complement(4385..5149)
                     /locus_tag="ACHA25_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8763049.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5257..5532
                     /locus_tag="ACHA25_001759"
     CDS             5257..5532
                     /locus_tag="ACHA25_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8763050.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(5880..7031)
                     /locus_tag="ACHA25_001760"
     CDS             complement(5880..7031)
                     /locus_tag="ACHA25_001760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8763051.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(7051..7773)
                     /locus_tag="ACHA25_001761"
     CDS             complement(7051..7773)
                     /locus_tag="ACHA25_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8763052.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(7766..9217)
                     /locus_tag="ACHA25_001762"
     CDS             complement(7766..9217)
                     /locus_tag="ACHA25_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8763053.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            9359..10441
                     /locus_tag="ACHA25_001763"
     CDS             9359..10441
                     /locus_tag="ACHA25_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8763054.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            10775..11713
                     /gene="yidC"
                     /locus_tag="ACHA25_001764"
     CDS             10775..11713
                     /gene="yidC"
                     /locus_tag="ACHA25_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8763055.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(11894..12298)
                     /locus_tag="ACHA25_001765"
     CDS             complement(11894..12298)
                     /locus_tag="ACHA25_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8763056.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12314..13123)
                     /locus_tag="ACHA25_001766"
     CDS             complement(12314..13123)
                     /locus_tag="ACHA25_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8763057.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(13110..14000)
                     /locus_tag="ACHA25_001767"
     CDS             complement(13110..14000)
                     /locus_tag="ACHA25_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8763058.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(14013..14492)
                     /locus_tag="ACHA25_001768"
     CDS             complement(14013..14492)
                     /locus_tag="ACHA25_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8763059.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(14509..16275)
                     /locus_tag="ACHA25_001769"
     CDS             complement(14509..16275)
                     /locus_tag="ACHA25_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM8763060.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(16321..17262)
                     /locus_tag="ACHA25_001770"
     CDS             complement(16321..17262)
                     /locus_tag="ACHA25_001770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8763061.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(17259..18254)
                     /gene="lacD"
                     /locus_tag="ACHA25_001771"
     CDS             complement(17259..18254)
                     /gene="lacD"
                     /locus_tag="ACHA25_001771"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8763062.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(18247..19440)
                     /locus_tag="ACHA25_001772"
     CDS             complement(18247..19440)
                     /locus_tag="ACHA25_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8763063.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(19458..20186)
                     /locus_tag="ACHA25_001773"
     CDS             complement(19458..20186)
                     /locus_tag="ACHA25_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8763064.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         5965 a         4442 c         3609 g         6562 t
ORIGIN      
        1 ttaagaaaaa atttacaaat cgaaaggagt tcattcaatg aatcttacta gagacctgat
       61 atttttagtt atctatattt taggaatgtc aggcatcctc tattggcgca agcgccaaag
      121 cgatcccgct ctattctttt attgtgggac gtggactttt atgatgatgg tcacttactt
      181 cgtcgtttta gaagtttttt atcatacatt ttattttttg attccattgc tatttttcag
      241 tatttttgca tttttctatt ttaaggaaaa acgtaggtta atcaatggcg tactttttaa
      301 tacgtttttg attgtctttg gtggttatct agggtggcta ctaatagaga cgcaaaatat
      361 ctttatcggt gggttattgg ctttgatcgg gataccaata ttaattcttt tggcaatcgg
      421 tatctacatt ttgatcattt ttttattttg gaatggtgtt gtagtcatga aaaaagaatc
      481 ccgttctttg gccaaccttt tgactctctt gttaggagtc gctttgacta tctttttgat
      541 attggactat acattagtcc attggctccc acaatgggca aatgctctat ttgctgtcat
      601 tccgtttatt tttgcttatc tactcatcgt tttctttaat tttatgactg tttcaatttt
      661 gtatcagttc aaccgtccaa ggtataacca agattatatc gttgttctcg gcgctggctt
      721 gattgatggt gaacgcgtat ctcccctact tgctaaaagg atcaataagg cgatcgcatt
      781 ttattgggca caaagtaaag caactttgaa tccgccagtc ttgctcatgt caggaggaaa
      841 aggaaccgat gaaaaagtag ctgaagccat tgcaatgaaa caatatgctt tagaaaaagg
      901 gattcccgag cgtgatattt tagtagaaac gaattccaca acgacgctag aaaatatgct
      961 gttttcaaaa gaaataatgg atcagcaaat gaatgggcct tatcgcgcga tttttacttc
     1021 aaataattac catatcttta gagctggact atatgcgcga caagcgaaac tcaatgcaaa
     1081 cggaatcggt gcaaaaactg cactatacta cttaccgaat gctatcttaa gagaatttat
     1141 tgccatcctt gtcttacaca aaaaacgcca tcttatcatc tgtggcttaa tgattggctt
     1201 tgcagtattt tctgctctta ttctcaaatt tgctcaataa aaaagagcgt gggacaaaac
     1261 taaaaatcag ttttgccaca gactctaact ccgaataaac ggtgggagca gaagcaactc
     1321 cttcggaaat aagcgaaatt cacaaaaatt taaaaagcaa ttttcgtgaa tttcttctta
     1381 tttctcggag ttaaacactt ctgtcccaac ctctttttat ttctcttttt gtcgtgagat
     1441 tcttccaagg atcggtagga acaagattcc ttttttcact tcaacttttt gcgcagaata
     1501 gattccacta tttcctgaac acataaagct aatcaaacag atcataaaga agtagctggc
     1561 tgcttgacct ccaaaaagtt cgatccccat aacaaagcaa gcaatcggtg tattcgttgc
     1621 tccagagaac accccaataa accctaatcc tgctaaaaaa ggtactgata catgaagaat
     1681 gggtgctaat gtcgcaccta aagttgcccc aatctcaaac aaaggggtca cttcgcctcc
     1741 ttggtatccc gcgccaagtg atagaacggt aaagccaagt ttcccaagaa aatcataagg
     1801 atgagcactt ccattaaatg catcttgtaa taaaggtaag cttaatccga gatatctttg
     1861 cgtctgtaaa atcactacta cgagtaccac gactgctcct ccaataaagt tgcgtaaaac
     1921 tggatggggg atccgctttg cataccaagc ttttaaccag acgatcgaac ggctaaagag
     1981 ccagccaatg ataccaaatg caatgcccgc aagcaacaat ttgaagaaaa gtgaagggtc
     2041 ccatgcagga atcgccccca tttgataatg ggtatgcttg acattgaaac tttccgttac
     2101 aaagttcgca aaaagtgctg caaaaaagct tggaaacagt gcttcggttc tcacttttcc
     2161 gattgccaat acttctaatc caaacaatgt cccagccaag ggtgtcccaa atactgagct
     2221 aaaacctgcg ctgatcccac tgatgacaag aatctcacgc tcgattttgt tcaattttag
     2281 ccaacgagca aaattctcag caagtgcccc gcccatttgg accgctgtac cctcccgacc
     2341 aacagaacca ccaaaaagat gcgtggtaat ggtgccaaat aaggtcagtg ggatcagccg
     2401 taatggaatc ttttcatttc ctccattccc ttgatcgatc actaaattgt ttccccggga
     2461 agaattgccc ccatacgtcg tatatagata agcaaaaagt cccccactta tcggcaaaag
     2521 aaaaagtaac caaggatgct ccagccgaat agctgtcaca tcagtcaaac tagttagaaa
     2581 gaatgcggat aaacttccca taaaaatacc taaaattgct gtgatcaata accatttgat
     2641 catatagatg atcaccttca atggatcagg taatcgattt tccatctcat ttcctcctca
     2701 aaaaaacgcc tactaaaaaa aggggtagcc ccgtttcttt agtaggcgtc attagttatc
     2761 ttacgatcaa cgtttaaagg cgaacaccat cacctattca cttcaccaaa taagatagca
     2821 tatttttcag aaaaatacaa tgaaatcacc gctcacgttt cactcgttct gtgaattcgt
     2881 aaaaaggagc taattgtacc aactgctgat tacattggtc actatcatga caaatataat
     2941 ttcctttttt cgtgtatgtt ccatctgaac cagtttttgt ggtagaaaga aagagtgaaa
     3001 catttgaagt tctttgacaa atcgaacaaa ttcctttgtt gattgtagga gataatgttc
     3061 cactgatccc ttttagtttt tcttcttcat aatagatcat aaattttttt tggctacctg
     3121 gatcattcca accaatataa gtatgttccc ttaaatccag ttgattccaa tctggttgtt
     3181 ttaacttttt cacttttcgg aataatttat cgatctgcgg ttttgtcggt tgtatgaatg
     3241 gaagaacgat cactttcaat tcctctaata gtcgttctgc tttttctttt tttagagtga
     3301 cgtctagtac cttttccata aattcattta cagctgtatt aggatgacca agtacctcgt
     3361 ggatcttttc cgcagctaat gcttggactg tttggactgt ctttggatca ttcactgaac
     3421 tataggcgct taacagctgc tcgacttctc gcttgataaa ataatattga tatggtttga
     3481 ttgttttttc catgactttg agtcctttct tctttcaaaa tcggctactt tgaagaaaaa
     3541 agtgatcttt ctatcaaagt gcctttagat aaagccctca aactctttta aataagatac
     3601 acatcgcatc ttatcatcac cccctttatt tttatgttgg ttatgtactt tttcatctca
     3661 acaaagtaaa cactaaaaag accaattagt caatgtctaa acaaaaaacc cgaggatttt
     3721 tttcaaaata ctcgggtcaa atacttagtt aactacctta aatcgattgt tctttaccga
     3781 aatatcgttg ccatttttgt ttcattgaag ctgtcattgg ttgagctgct tctttgactg
     3841 cacaatattt atccacaaaa gtaacgatat aaccttcttt atatttagga ggggcaatcg
     3901 ttgctcccca catatgtttc aaaataatat cacgctctag atctgaaagc tccgtgattt
     3961 tttcagcatt tttcacagcg atccttgggt gaacatacgc atgtgtacct tcatcgaact
     4021 tcgtcgtacg ccaatcgtag taaaataaat catgtaaaag gcctgctcga gcagtcgctc
     4081 ttgcatcacc gccccatttt ttcgctaatt tgtagctata gtaagacaca ctgattgaat
     4141 gctctaatcg tgtcgagtgg acatgttgcg tgtaatttgc taatttcttt actgcatcag
     4201 tttctaatag atcatttata taagacatat attcttcgtc ttcacgccat ttttctgtca
     4261 taattatcga tctgccacct ttttgaattc catttatctt tgaaattgaa cttagtatac
     4321 cactagtgtt cgtaatattc tagtgaggtt ttcgcaaaat gcagaaagct taatagttcc
     4381 ttaactatac ggagaagtga taaagcatga tgcccgctgc aactcctaca tttaaagact
     4441 ccgcatttcc ttgaatcggt atatacacat ttttatccgt tagcgccagc acttctggac
     4501 gtacgccttg accttcattc cccatcacga tcgcataatt ttctgttgtc tggatatcat
     4561 tcagggacac tgcttcttca ttcagttctg ttccataggt tataaatcct tgctctttaa
     4621 ggataggcaa tagttttgtt agatcctctt ggatgatcgg taaatgatag ttactcccct
     4681 gcatgcttct taatactttg gtactgtaga catccgccgt tcccttacct agtacaacac
     4741 aagatagacc aaaagcatcg gctgtgcgga tcattgtccc tacattgccg ggatcttgta
     4801 cgttgtcgag caacaaccaa ccaccttcaa aattcggtat ctctcttttt tcagcttcca
     4861 ccactgccat gatcccttgc ggagttggta gatctgaaac agttgccatc acttcttctg
     4921 atactagtgt aacagaaagc gcctgatttt cttcaagcca actagcccac tcagtcagac
     4981 cgcgttgcgt aataaaaagt tcgttgacct tcgcatttga ggcaacagcc tcctcgatca
     5041 ggtgaaaccc ttcaagcaga tattcccctt tttcttcacg atattttttc ttatgtaatt
     5101 tttttaattc tttgattctt ggattttttg ctgattggat ctctttcaca tttttcacct
     5161 caactgatta taccatggat tagatcattg cacttgttca gtagcaaaaa agtcggtatc
     5221 attaatttaa aaataaaaga aagaacggtg aataaaatga acaaaatcaa gatgaacgtg
     5281 caaggacgtg tacaaagcgt tggttttcgc tatatgacaa aaatcgtagc ggatcaatta
     5341 gggatcaccg gaacagtgaa aaatgaagat gacggctctg tcacgatcga agctgcgggc
     5401 gacgatgagg caatcaaaaa attcgtggaa gaagtcaaac gatcaccaag ccccagcgga
     5461 cgtgtccaat atgtagacct tcaggaaaat ccgatgatgg aggacagaaa aaagtttgtt
     5521 gtaataggct gaccttctag aaatgaatag gaaatgtcag aaaaatcgga ctgtctgtat
     5581 gtagatcgaa aaagtttgcc cctctctcca tcctagatcg acaaaaaaac attcttatta
     5641 gcgagtttag taaccagtga taggcatacg taaaagcggg aggacttcaa aaattttggt
     5701 tcaataaaat ggagtctata atattttatg gtagtggaaa atcgactttg attttccact
     5761 accacttttt cattgtcaaa tcctgacgct gcccagtcaa caagccaact ctcgaaagga
     5821 tcattttccc tctttgttct aatggatacc catagctgtt ttcaaatata cagttttatc
     5881 taaaaattta gttttgctaa acgatcaaag gcttcaccta agatttccaa tggctgagtc
     5941 gctgctagtc gaatatagtt gtccccgatc gtttccccaa acgcttttcc agggacaaaa
     6001 agtacgccgc ttttttctaa aacctcttcc acaaacgtta ctgaatcgag cccactctta
     6061 ctgatattaa taaaagcata catgcttcca gaaacagaag ataaagataa gtaagggatt
     6121 tctgccactc ttttctcaat ataagctaat cgttctttga atacttcaac aactggcggt
     6181 acaagctgtt catagtgctt taaggcgtag attgcacctc tttgagaagc agtcggtgcg
     6241 gaataagtga tgttttcatt gatcaaccca gcaatttgat tgatgtatgc tggtgcgatc
     6301 atgtacccta agcgccagcc agtcatcgca aaccccttcg atacaccact gacagtgatc
     6361 gtattatcgg gcgcaaatcg cgccatcggt acgaacttct ctttaaaggc aaagtcttca
     6421 taaatctcat cggaaatgat atagagttca ttttcgattg cgacttcagc gatttttttc
     6481 aacgtgcttt cagaaaaaac agctcccgta ggattattcg gcgaattgat gacgatcacc
     6541 tttgtacgat ccgtaatcgc tgcttttaaa tcttccacat ccaactggaa gcccttttct
     6601 tcataggtag gaacaaaaac aggcttgcct ccagataaaa tgacttgttc ccgatatggt
     6661 gaaaaataag gttcatggat gatgacctca tcctctggat ctagtaatgc ctgtaaaact
     6721 agatacatcc cgtgcattgc tccgactgta gctcgaactt ggttgcattc aacgctcaat
     6781 ccatatttct tttgataaaa atctacgacc gttgacaaga attctttgct gccatcaggc
     6841 gcagtataat tagtagcacc attttttaca tctgtataag ctgcttcaat aatcgtcgga
     6901 tcagtgatca agtcaggatc accaatcgat aaatcgataa tatcctcttt ttcctttgcc
     6961 aaaatagcaa tattcatcaa cagattttct gctggttgct gaaaccggcg tgcaatctta
     7021 cttttgtcca tcttacttcc tccatctttg ttacaacact ttgttcaaga aatcctgtgt
     7081 tcgactattt tgcggatttt caaaaatctc tttcggtgtt ccctcttctt gaatgatgcc
     7141 gtcagccata aaaatcacac gatcggcaac ttctttagca aaacccattt catgagtgac
     7201 cacgaccatc gtcatccctt ttttagctaa atcctgcatg actgccaaca cttcaccgac
     7261 catttcagga tcaagtgcag atgtaggttc atcgaaaagc atcacatcag gattcattgc
     7321 taatgctcga gcaattgcta cccgttgttg ctgtccccca gataaactgg taggataact
     7381 gtccactttt tcctttagac caacttggtt caataatgat agagcatgtt cttttgcttt
     7441 tttagggtct tcttttttga tttttacagg tgtgatggtc aaattttcga gtacattcat
     7501 atggggaaac aaattaaaac tttgaaatac cattcccatt ttttgacgaa tttgattgat
     7561 atcgctgtca ggggctgtaa tgttttgtcc cttaaaaaag atttcaccag aagttggttc
     7621 ttccagcaga ttcatacaac gtagaattgt acttttccca cttcctgaag ggccaatgat
     7681 caccactact tctccttctt tcacagaaag atcaattgat tttaagactt cattctcgcc
     7741 aaaacttttt gacagttttt ttacattaag cattcagacc cattctcctt tcaactaaag
     7801 ctaatatacg ggaaatactg aaggtcaaca cgaaataaat caaagagacc aacacatatg
     7861 gtaggaatgg tttgaagcta gcaccttgta ccacgcccgt ttgaaagatc aattccgata
     7921 ccccaatgac cgaaacaact gaggactctt tgatgatcgt gacaaactca tttcctagag
     7981 ctggaagaat atttttgatt gcttgtggga agatgatgaa acgcatggct tctttctggt
     8041 ccattcctaa agaacgtgca gcttccatct gtcctttgtt gaccgcattg atcccggcac
     8101 ggacaatttc ggctacataa gcagcactat tcaacgagac agcaatacaa cctgctacca
     8161 gtttcgatag atccaatccc agtactcctg ttccgaagta aacgataaag atttgaacta
     8221 agagaggtgt tccccgtacg tattcgatat aaataatggc taatgccttt aaaaatttat
     8281 tcttacccaa tttcatcagt gcaagcaaac taccaaagac cattccaaac aatacaccca
     8341 caaaagctaa taaaatagtg tattttgttc caatcaaata atacggaccg tatttttgaa
     8401 gaaaggactg atcatcttgg aacatcaatt gattcgcttg tttttgataa tctttcagca
     8461 agccgtcgtc attgatcgtt ttgattgacg cattgacatg atttttcagt tctggtgtat
     8521 tttttgacat cgcaatagct gtggtttttt cgccttgcgc aaattttact ggagtcaaag
     8581 ctactttctt attctgttgg gcatatgctt ctgcaactgg ttgttccagc acaacacctt
     8641 caactttttc attcagcaaa ttattgatca gatcaggaac tttttgcagg gaggtcaact
     8701 ctgcaccagt cagttcattt ttcactaact cctcttgtgt ggtttgcttt tgcgccccaa
     8761 cggctaagcc agaaaagtcg gctgtcgtct taaatttatt tttgtcctct ttacgaacga
     8821 gtactttttg ctgtaccacg aaatatgggt cagaaaagtc aacttcttgt aaacgttctt
     8881 cagtcaccgc cataccagat ataactaaat cgatcttgcc agttttcaac gcacctaata
     8941 atgcatcaaa accaagttct tctactttta gtttcacacc catatccttt gcgatctttt
     9001 cggcaatgga tacatcaaaa ccaacgattt tatccttacc atccacattc gcatggaatt
     9061 catagggcgc atagtctgca gataggccaa cgactaattc tccactttgt tttatccgct
     9121 cgtaaatttc atctttttct gcaccttgta cttttactgg taaaaaaacc agtagaaaca
     9181 tcagcgccac taataatatg tttattcctt ttttcatcca tttggccacc tttgaataat
     9241 aatgtaatca gaaaagctat aaaagaataa atattcataa ataacttgct aatatactag
     9301 cataactatt ataataaaaa aagattaatt taaaaaaata atggaagaag ttttttatat
     9361 gacaaaaaat ttaattgttc gcggtgcccc tcaagagtat gaaataaaag aaaacgcatg
     9421 ggattcactc cctgaacacc tttacaaaag gcatattgac aacgttttga ttctccatgg
     9481 aaaaacatct tggaaagcag taaaagatgt ttttccagat ttatcaataa caaaaatttt
     9541 tgaatattat ggtggagaat gtaccgatcc acttgctgac agttttgttt ctttatgcaa
     9601 aaagaaggat ttcaatggta ttattgcagt cggcggcggg aaagtagcag atctaggaaa
     9661 attgattgcc tataagacaa acttaccaat catcatctta cctaccttag ctgcgacgtg
     9721 cgcagcttat actccactta gtgtggtcta taatgaagaa ggagtgatgc ttcgctatga
     9781 tatgttccct aaagcaaatg atttagtgtt gatcgatcca aagatcattt tgcactcccc
     9841 tcgcgagatg atgattgccg gtattggtga caccttagca aaatggtatg aatctcacgc
     9901 aatcatctct cagttaacta tcaagcctat cgaagtccaa gtagcggaat ttgcagcgtt
     9961 acgatgccgc gaaatccttt tagagaaaag tgcagaggca atggaagcga tggaccagca
    10021 aaaaatcaat caagctttta tcgatgtcgt ggaaacaaac attttattgg ctggtatggt
    10081 tggtgggttt ggcgatgact acggccgaac agctggagct cattccattc acgatgcgat
    10141 gaccgtactc cctgatagcc atcagcaact tcatggaaac aaagttgcat acggtatcct
    10201 tgtccagctg atgatcgaaa ataaattagc agaaatcgaa cacctactgc cgttttatca
    10261 tcacttgaac ttaccaacga gtttagctga tatgggttta catttaacag aaagtgacta
    10321 tcaagccgta gctgaacgtg catgtttacc tgatgaaatg atccacttca tgaaagaaac
    10381 gatcacacca gcactggttg tccagaaaat gaaagagtta gaacaagtga cacagggata
    10441 gttagcccct tataccatgg cagtcagctt ttttatggat cggctcaatg gtaaaaaaaa
    10501 agaagtggaa aaaattgaca ttccacttct tttttttgcg ctaaaattat gatttcaagg
    10561 aaatatccgt tgctcatctt attttagaga acggaggtgc taagtatgct ttatactgaa
    10621 gtatttttag ctggattctt ctttttaaca ttaagcgtct tttttgatcc tccaatcggt
    10681 tatttctatg aaatttctat gaagttgatt gaattatgag cgctttttta gtatagttaa
    10741 gcttgtgtaa aaattataaa agaggaagta ttttatgaat aaatggaaaa attggttatt
    10801 gggctctggg cttgtcacga tcatgctttt cttgtccggt tgtgtccgca tggattcaaa
    10861 tggtaaccct gacacttcag gaatcattta ccgtatattg gttcacccaa tgggacaagc
    10921 gattacatat ttagtggaaa actttaattg gtcctacggc tgggctgtta tcttgatgac
    10981 agtcatcgtt cgtatcatca ttttgccatt aggtattagt caatcgaaaa aaacaatgat
    11041 ccaatctgaa aagatgcaag cattgaaacc acaagttgaa gctacgcaac aaaaattgaa
    11101 agctgcgaca actcgagaag aacaaatggc tgcacaagct gaaatgcaac aagtttatcg
    11161 tgaaaatggc ttaagtatga ctggtggtat cggttgttta ccgcttctga tccaaatgcc
    11221 gatcttttct gcgctatatt tcactgctcg ctatacagaa ggcatccgtg aatcaacctt
    11281 ttacgggatc gacctaggta accctagctt agttttagta gcgattgccg gtattgctta
    11341 tttacttcaa ggctatatct caactatcgg tatccctgaa gaacaaaaga aaacgatgcg
    11401 tacgatgttg atcgtatcac ccgctatgat cgtcttcatg tcgatcagtg ccccagctgg
    11461 tgtcactctt tactgggtag tcggtgggat ctttagttgt ctacaaacat tcattacgaa
    11521 tgtcatcatg aaaccaagat taaaagcgca agttgcggaa gaattaaaac aaaatccgcc
    11581 aaaacaagtg gtgacgcctc gtaaagatgt cacacctgct gaacctgcga agcaaacaaa
    11641 aaaacaagta caagcaccaa aaaacaatgc gaagaatggt cggaatgctg gtaaacagca
    11701 aaagcgtaaa tagttatttg taactagctt cttcgatcga cccagatcat ttctgatttt
    11761 atgacgataa aagccaacgt actgtgaaat atagaaaact atatttcgcg gtacgttggc
    11821 ttttttatac acggcctctt tatacatccg tatttatatc cagttgtatc accagtcaag
    11881 aagttgtgct tgattattct tcttcatcgt ctcccatgcc agcaatcact tggttcactg
    11941 aaacgatact ctcacgaccc gaaccaataa aatctggttc tgctccaatc atttgcgaat
    12001 tgatgaattc gatgaccatc ggcaaattca tgccagcata tagttcgatc gtttcacctt
    12061 ccatgattag cttggaaacg gtatttgcag gtgtcccacc caacagatcg caaagcacta
    12121 cgtaatcttt caatggtgct gtaacggaga ggaatctttc tttatacgtg tccgcgccat
    12181 cttcaggcaa taaagggatc gtatagatat tttcttgtgg tcccatgatc atttctgtac
    12241 tgttttttag ttcttcacaa aatttaccat gactaatcaa gactagttgt ctactcatct
    12301 ttccaattct cccttatttc gaaatcacac ctaatgcaga taatgctaca catacgatca
    12361 atacgatgaa tattgctcga gtaggtgtca tatttttctt acctaacatc caataaacgc
    12421 caccaacgat tccagctggt aaaagtttag gtaagatcat atcgatatag ttttgcatat
    12481 tcaacgttac atccccaatc gttggtgccc atgagaattt aacattgatc atggttgcta
    12541 ctaacgctcc aaccatgaaa actcctagta aagtagccgc atctgtcaat gcagtcaagc
    12601 gtgattgcat cgtggtaact agagaaatcc cttctttgta ggcaaattct agttgtttcc
    12661 aacgaaagac catgacagcg acctgagcaa ggatccagat aaggatacct actggattac
    12721 catctgttgc catatttgcg gctaaagctc caaatatggt cgggatcaat gccgcaaata
    12781 cagaatcacc aattgcagcg aaggaaccca tcaatcctgc tttcatccca gaaacagttt
    12841 cctttgaaga gactccttct ttttcttcga tagcaagatc gatcccagta atgatcgtgt
    12901 taaagaaatt agacgtatta aaaaattgcg tatgtgtttt catcatttct tttaattctg
    12961 gtgtatcatc accatagatt tttctaagtt gtggcaagat agtatacaga taacctgaac
    13021 cttgcattct ttcatagttc caacctaatt ggaaagtaaa caaacttcta cgattgatct
    13081 gcttaaaatc ttctttggtc aatttataat tagatttcgt catcttcgat ttccccctca
    13141 tcagattgtg attgctgact tactactgga tgttggtcat tcttttgatc attcttataa
    13201 ctgatcgttg ccagtgctag accaatcaat gccacagcaa gcatcggtaa tgaattgaat
    13261 gttcctggga aatctttgac cacgctagcc acacttgttc ctaacaattg gacattcgag
    13321 aacactgttg ctaataacgc tgtgatcgta aatcctaaaa tcagataaga aatgtgtttc
    13381 tttactggta ggtagcgtag taaaattgca aacccaactg ctggtaagac tgcccctgca
    13441 acagataagc catctcctag ccattttagg tcgccgttta aaacagccac aacattttgg
    13501 actaagccac caccgaatgc tagggccaag aatacaggaa ccattctgga aagtgcccaa
    13561 ggtaatgccc ccatcaagaa gttccgttca attgctttgt aattcattgt ttccacatgt
    13621 tgatcgatcc gatgtgcaaa atacgtattc gcaaaacgtg ccaaaatatc taattggatc
    13681 atcaaacttg ctacaggaac cgcgatggcc gcaatcgctt gttctgggtt catccctaga
    13741 gaaatggaga aagctgttgc taagatcgtt ccagagttgg catcgatttt tgatgcacca
    13801 ccaaatgtcc ctacccctaa aacagtcaac tgcatacttc caccaatgat taaacctgct
    13861 tttaaatcac ccattactaa cccagcatat aatcctgcaa aaactggggc gctcattgaa
    13921 gaataaatct gtaattcatc taaaatttga taccctgcat ataatgttaa taataatatc
    13981 tgccaccatt gaatatccat gaatcattcc tcctatttga ttaaaagact cataaagtct
    14041 tctgctttgt catttggcac catttgagcg atcaaatgaa cgccaagctg atctaatttc
    14101 ttgaagacct ctacatccgc atctacgaca ttgattgatt ttgtgatgga acgtgtttca
    14161 tctgtttgtg acatattgcc gacatttaat tctttgatcg gtacaccttt ttctactagc
    14221 gctaagatac gatctggttt tttcgctacg atcagcactc gttgagaatc atatttacct
    14281 gctaaaatat ttgcggctgc tttttcaata ggtaaaatac ttaatttgac tccagctggc
    14341 gttgccaact ttaacccact tttttcgatc gcatttgttg ctacctcatc gtctacgacc
    14401 atgatccgac caatattcaa ctttgtcgtc caaagatttg ccacttgtcc atgtatcaaa
    14461 cgtccatcaa ttcttactcc aataattgcc atcatccatt ctccttttct aacttttttc
    14521 tattggttct ttgacaaaat ctataacttc actgaatcta ccttctgttt caaagataac
    14581 gatttcattt tgtccttttc taaataaacc ctcgggaaca aacagtgaaa gaattggtcc
    14641 tacttcccaa aaacgaccaa tattcgtgcc attcacccaa acgatccctt tcccaaactc
    14701 actaaggtcc aaataagtat caccgatttc tgacagttcc gcttcaaagc ggtaaaatgc
    14761 tggttgacct gcttcccatt tttttgaaaa atcgatgtgt tctgtagtct ctaaaggtaa
    14821 acaatactgt gtccattctg tgataaaatg aagatcgctc atcactcctg tacgtattcc
    14881 ttttctttgc gtatcagcta gcagcttgtg tccatagttg acccgtccca tattttcaaa
    14941 taatagatca atttgatggt cttctttctc aagtgtcaat ggaatggtct caccgatctc
    15001 tgtttggtat tgaatcgctt gcttttcttc atcgagaaaa acatggaccc gatcacgtcc
    15061 gtcgatgatc cgtaattttt cattgaccgc atcttttttg atcgttgttc gatacaatag
    15121 gtacccagta ttctgaccca attcttccat tgtttgagga taggtcgagt ggatcggctt
    15181 gctgatggta tccagtgttg cgaaaaagct gactttttct ttcaagttga ttcctttttt
    15241 gacgattgtt tcttttatcc atggttccat ttgctgcatt tctggaaaat tttctgccat
    15301 cattcgtttc agagcaaaat atttttccgt tggatttccc tgctcatcca atggtgcatc
    15361 gtaatcatag gaagtgactt gcggcaaatc aatcgcgcct ctggctgaac agccattcat
    15421 aaacccaaaa ttggttccgc catggaacat atagagattg atactgccga tctgtaatgc
    15481 ttctttgact gcatccgcta attcttccgg atctctttta atgatcggct ctttccaacg
    15541 attaaaccag ccatcccaaa attccataca catcaacggc cattttttac catgtgcttc
    15601 aaaaaactgg gccatatctg caaaattttc ttttgctttt gagccaaaat tccctgtcgt
    15661 aaaaacatca tcgtcaatca aaatgcctgc tcttaatgtg gcttgccaag gtccatcaga
    15721 agtgaatagc ggtacatcga ttccaccttc ctccaagtat ttttttactt ggcgcaggta
    15781 tgctttctct tcaccatacg aaccgtactc attttctacc tgcaccatta agacgttgcc
    15841 accatgagtg actagatgag gaacgatttt aggtagtagc gtttggtaat accgtttgac
    15901 atacttcatg aagcgaggat ccgctgaacg aatacggcac tcctttgtca atagccaagc
    15961 aggcaagcca ccaaattccc attctgcaca aatataaggg gctggacgta cgattgcgta
    16021 taatccaagg gattgcgcta actctagaaa atgggaaaga tccaatattt tcgaaaaatc
    16081 ataggttcct tcttcttttt catggagatt ccaaggcaca taggtttcca cagtattaaa
    16141 tccaagcgct ttcaaattgt gcaatgaccg ctcccaattc gtcggatgca ctcggaaata
    16201 atggatggca ccagataaaa ttttgaaagg ttgaccgtct aacaaaaact cttcttttat
    16261 ttcaaatcta gtcaagcaaa tttcctcctc gtttttagaa accgctttct aattttataa
    16321 tcaacgaatc tttctgacgt tgatttcccc cctaaaataa tcgactttca caggatcgat
    16381 ccatcctgtt ttttcttgtt gtgcattcgc catcccacag gccatcgcaa acgcaagtgt
    16441 ttcttcaatc gtatagctct ttgatagagc atgtgctaag cctgcaatag tcgaatctcc
    16501 tgaccccaca ggattcacca ccgcgatttt aggaatcgta acctgataga agtgtctccc
    16561 tactttagcg attgctcctt ttgcgcctag cgaaagaacg atccaattga ttccttcaaa
    16621 aagcggatga tccaataatg attggatttc atctaacgaa gtagccgaag aaattgtttg
    16681 gtggaccaac tctcctaatt cttccaaatt aggtttgatc aaaaatggtt tttccttgcc
    16741 ttccaagact tcaagcaagg tttgaccaga agtatctaac aatactcgtg ttcctttttt
    16801 attcgttagg gcaagtaact ctgcataata acttttagaa aatccttgag gcagactacc
    16861 cgaaatcgta aagatatcag ttgtttccat tagtcgtgca aaatgctcgg taaatttatg
    16921 ttgctcttct tccgtcacaa caggaccgct ttccaatagt tctgtttgat tcactccatg
    16981 taaaatagca atcgaatctc tcgtttcccc tttgataggt aaaaattgat gcgttatccc
    17041 tgattgatcg agttgttctt tgatatatgc tccatgctgc ccgccgacta atccagtagc
    17101 taaaacatca tctgctaata aatgaaggac acgagcaaca ttcaagcctt ttcctccagc
    17161 cgttttgttg accgttttga cccgattgac ttggtcgatc cgtaattcat ccaacgtgta
    17221 agcgatgtca attgaaggat tcatggtaac tgtcgtgatc attttgctgt caccgaatct
    17281 tcttttactt tgtcaaacca aggggtggcg ttttcattta tcactttatt caacgattcg
    17341 atattttgtc gcccttgtgt tctcaaccac tcaatcgcac cttctctacc attttcaaca
    17401 tatgctttga ctccatcttt ccaagtcgca cgtccgcata acacaccgtt gaatgttgaa
    17461 ccagattcat gagcaaagat caatgtttgt tggaacaatg cagtcgaaac ccctgcactc
    17521 aagaagataa atggtaacgg tgttgcatca ctttgttcct tgaagaatgc cgctgcttct
    17581 tttttactat acgcaatttc accatcagaa taaccttcta catagttcat attgactggc
    17641 acttctactt tcaatacatc cactttatat ttaggatcag aaaattcttt catcatctcg
    17701 atcactttat gcggtttgat cttcgcatat tctaatgaag tgctgtcatt ggttttggca
    17761 tcatacgaaa ccaattctag ataaaacgga cgcttttcac ctgcacattc actgcctaaa
    17821 cgttcgataa atacatgttt caaatgattg atctctgggt cttcatctac atcataataa
    17881 agcaaaaact tgatcgcatc ggctgtttgt tcctttaatc taagcgtaga ccaatccgct
    17941 aataaatcag gcagacgtcc tggagttgtc gcatcataac ccgttttttc ataggctaaa
    18001 agtagtcctg cttcctgatg tctggcttta gcagctggca atccatactc tggatctagt
    18061 aaaatagcag aagcataagg cgttaattct gctgatacta attctttgaa ttcagtgatc
    18121 tgttgcgccg ttggttcttt tcctaacgct ttgatcatct ttttcaaagc accgcgttga
    18181 tcaattgcca atgcactgat gattccttct tttgttgata aactcttcat agctgcaatt
    18241 ttttcttcat tcatccttcg tttccccttt gttcacatat tcatggataa tgacaccttt
    18301 gaccactcga ttgactgtac ctgtaggcga tggtgtatct ggtttattcg ctacttttat
    18361 cgacgtatcg agcgcaatga tttgggctgt catgatcata ggtaaggcaa gatacgcatc
    18421 aggtaggctg atcgaagctt ctaacaaaag acttgtacct gtgaaatcga tgtttcctgg
    18481 ttgggcgatc gcgatgacac tcgtggtaat ttggtcatct tttatttctt ctagaatatc
    18541 taagtcataa tttcttgtat acggttggtt attaacaaaa ccaataacaa gtgtttcgct
    18601 atttaaaaac gattttggtc catggcggaa gcccatagaa gaatcaaaga tcgttgccac
    18661 tttaccggct gtcaattcca atattttcaa ttgtgcttct ctcgttgcac cagccaaact
    18721 tcctgaacca agataggcga tacgttcaaa tgacttaggc aataattttt ggatttcttg
    18781 atctcgtgaa attagatctt ctccagttct gatcaattgt tgaagataac tttctttttt
    18841 ctcaacggca gtttggtcga atacccataa cgcactcaac aacatgcacg taaaacttcc
    18901 tgtcatggca aatcctgcat catttgattt ttctggcatc agtaaaagat aatttcgttc
    18961 atctgtcgct gcattctttg ctaactgacc ttctttagca caagtaatcg tcaaatgaaa
    19021 caatgtcttg atttgttcat tggcaatctt gactgttgcc acactttctg gactatttcc
    19081 actacgtgca aatgacacca gtaatgtcgg ttcatccgga aatagaaatt cttttggtga
    19141 agacacgata tctgtcgtac caaagctatg aaaaataaag tgatcgttat caccgtattc
    19201 atttaaataa ggtactgctg tatccccgac atattgtgat gtgcctgcac cagtaaaaat
    19261 cactcgaact cttttgccat ttgctgcttt ttttatctgg tcaaaaaaca atgctaattt
    19321 ttcttgattc tgtgctaata tctcagctgt ttctttccat aattgcggtt gctgtttgat
    19381 ttctcgcgta gttatttccg cacccaactc ttttaattta tccttcgata gttcaaacat
    19441 gttgttttcc ctctcaatca ttttcggacg tggcggatct tataatgaaa ctggtctgct
    19501 ctcgctacac tatccgtata ttcaataatt tcatttttca cattataggt cgttcggctt
    19561 agatataata ccggggcacc atcagcgata cgaagcggat cagcatcttt cttttgaacg
    19621 atacttgcat aaaattcttc atctgctaat tggacttgct gttcataatc ctgagaaaac
    19681 acatcataca atggcttact ttcaagtaat ggcagatcaa gaccaataaa tagttttgca
    19741 ggtaaataag aacgttccac catcaatggt tcgtcatccg ctaaacgaag tcttttcaat
    19801 ttgaaaactg tttcgcctaa attgatgttt agattcttgg caaccttttt gtttgcttcc
    19861 atttgcttga attccaagat gactgtatta ggcacccgtc ccaattccct catctcttct
    19921 gtaaaactat acgctcccgc cagatcagac gacttatctg atagatcaga cacatatgtt
    19981 ccttttccgt gtcggcgata aatataccct tgcgtctcta attcttgcaa agcaaggcga
    20041 accgttgtcc gactcactcc ataatgctga caaagttctc tttcagataa caatttgtca
    20101 tgaggtgtca tctgtgtttc gatacgttct ttcaatacgt caactagttg ataatataat
    20161 gattttcctt tacttgagct ctccataaaa atacctcttc tttactggtt ataaccacaa
    20221 cacaagaata cccacatatt cctcatttgt caaagggttt ttggcgtaaa aaagctatta
    20281 taacaaggtt tcaaggtaat ttagaactgg tatcaaaaga tacctcaaaa ataccagttg
    20341 acaaataaaa aacaaaaaaa agctgaatct tggcaaagat tcagctaaat ggtctagaga
    20401 agtaaagaaa aaatattctt tatacatctg tggctatttc tactatcgac aagcaaatgg
    20461 gcctaataga ctactatgta aataaaaatg gctttctcta aataaagtag gattttttat
    20521 tttttagata acttttctct catgccggtc tgatagtcga aattttgttg tacatttt
//
LOCUS       DAYYFD010000053        19631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000053 DAYYFD010000000
VERSION     DAYYFD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.053"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHA25_001774"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHA25_001774"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8763065.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACHA25_001775"
     CDS             complement(1174..1869)
                     /locus_tag="ACHA25_001775"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8763066.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACHA25_001776"
     CDS             complement(1882..2598)
                     /locus_tag="ACHA25_001776"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8763067.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACHA25_001777"
     CDS             2928..3536
                     /locus_tag="ACHA25_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8763068.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACHA25_001778"
     CDS             3861..4184
                     /locus_tag="ACHA25_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8763069.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACHA25_001779"
     CDS             4366..5736
                     /locus_tag="ACHA25_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8763070.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACHA25_001780"
     CDS             complement(5866..7167)
                     /locus_tag="ACHA25_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8763071.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACHA25_001781"
     CDS             complement(7521..9512)
                     /locus_tag="ACHA25_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8763072.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACHA25_001782"
     CDS             complement(9656..9922)
                     /locus_tag="ACHA25_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8763073.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHA25_001783"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHA25_001783"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8763074.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHA25_001784"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHA25_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8763075.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACHA25_001785"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACHA25_001785"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8763076.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACHA25_001786"
     CDS             complement(14082..14603)
                     /locus_tag="ACHA25_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8763077.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACHA25_001787"
     CDS             complement(14606..15448)
                     /locus_tag="ACHA25_001787"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8763078.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACHA25_001788"
     CDS             complement(15441..16166)
                     /locus_tag="ACHA25_001788"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8763079.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACHA25_001789"
     CDS             16342..16638
                     /locus_tag="ACHA25_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763080.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACHA25_001790"
     CDS             17206..18798
                     /locus_tag="ACHA25_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8763081.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACHA25_001791"
     CDS             18811..19551
                     /locus_tag="ACHA25_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8763082.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5618 a         4028 c         3724 g         6261 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaga
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt c
//
LOCUS       DAYYFD010000054        19451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000054 DAYYFD010000000
VERSION     DAYYFD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.054"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHA25_001792"
     CDS             127..3654
                     /locus_tag="ACHA25_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8763083.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHA25_001793"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHA25_001793"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8763084.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHA25_001794"
     CDS             7442..7783
                     /locus_tag="ACHA25_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8763085.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHA25_001795"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHA25_001795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8763086.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHA25_001796"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHA25_001796"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8763087.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHA25_001797"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHA25_001797"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8763088.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACHA25_001798"
     CDS             12994..13731
                     /locus_tag="ACHA25_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8763089.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACHA25_001799"
     CDS             13745..14560
                     /locus_tag="ACHA25_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8763090.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACHA25_001800"
     CDS             14573..15220
                     /locus_tag="ACHA25_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8763091.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACHA25_001801"
     CDS             15236..15892
                     /locus_tag="ACHA25_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8763092.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACHA25_001802"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACHA25_001802"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8763093.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACHA25_001803"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACHA25_001803"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8763094.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACHA25_001804"
     CDS             18234..18752
                     /locus_tag="ACHA25_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8763095.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHA25_001805"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHA25_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8763096.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4177 g         5392 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag c
//
LOCUS       DAYYFD010000055        19196 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000055 DAYYFD010000000
VERSION     DAYYFD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.055"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..1546
                     /locus_tag="ACHA25_001806"
     CDS             119..1546
                     /locus_tag="ACHA25_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763097.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1638..2003)
                     /locus_tag="ACHA25_001807"
     CDS             complement(1638..2003)
                     /locus_tag="ACHA25_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8763098.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2000..2365)
                     /locus_tag="ACHA25_001808"
     CDS             complement(2000..2365)
                     /locus_tag="ACHA25_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8763099.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2557..2805)
                     /locus_tag="ACHA25_001809"
     CDS             complement(2557..2805)
                     /locus_tag="ACHA25_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763100.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2804..2932
                     /locus_tag="ACHA25_001810"
     CDS             2804..2932
                     /locus_tag="ACHA25_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8763101.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2989..4683
                     /locus_tag="ACHA25_001811"
     CDS             2989..4683
                     /locus_tag="ACHA25_001811"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8763102.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4783..4956
                     /locus_tag="ACHA25_001812"
     CDS             4783..4956
                     /locus_tag="ACHA25_001812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763103.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5334..6467
                     /locus_tag="ACHA25_001813"
     CDS             5334..6467
                     /locus_tag="ACHA25_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8763104.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6498..6818
                     /locus_tag="ACHA25_001814"
     CDS             6498..6818
                     /locus_tag="ACHA25_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8763105.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6847..7311)
                     /locus_tag="ACHA25_001815"
     CDS             complement(6847..7311)
                     /locus_tag="ACHA25_001815"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8763106.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7443..8696)
                     /locus_tag="ACHA25_001816"
     CDS             complement(7443..8696)
                     /locus_tag="ACHA25_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8763107.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8725..9042)
                     /locus_tag="ACHA25_001817"
     CDS             complement(8725..9042)
                     /locus_tag="ACHA25_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8763108.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9068..9388)
                     /locus_tag="ACHA25_001818"
     CDS             complement(9068..9388)
                     /locus_tag="ACHA25_001818"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8763109.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9852..11267)
                     /locus_tag="ACHA25_001819"
     CDS             complement(9852..11267)
                     /locus_tag="ACHA25_001819"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8763110.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11284..12600)
                     /locus_tag="ACHA25_001820"
     CDS             complement(11284..12600)
                     /locus_tag="ACHA25_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8763111.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12750..13448)
                     /locus_tag="ACHA25_001821"
     CDS             complement(12750..13448)
                     /locus_tag="ACHA25_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8763112.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13737..14726)
                     /locus_tag="ACHA25_001822"
     CDS             complement(13737..14726)
                     /locus_tag="ACHA25_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8763113.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14806..15639)
                     /gene="msrA"
                     /locus_tag="ACHA25_001823"
     CDS             complement(14806..15639)
                     /gene="msrA"
                     /locus_tag="ACHA25_001823"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8763114.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15814..17448)
                     /locus_tag="ACHA25_001824"
     CDS             complement(15814..17448)
                     /locus_tag="ACHA25_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8763115.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17435..>19196)
                     /locus_tag="ACHA25_001825"
     CDS             complement(17435..>19196)
                     /locus_tag="ACHA25_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763116.1"
                     /translation="TQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLSTLY
                     SVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIP
                     AVVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRT
                     VDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCS
                     ALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSP
                     FKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGV
                     VSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWL
                     IIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAI
                     KERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGK
                     VVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPN
                     KLKDSRYVTKKEINKKNENK"
BASE COUNT         6093 a         3755 c         3192 g         6156 t
ORIGIN      
        1 aaaacctttt ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag
       61 aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag gctctactat
      121 gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg ggcaattcta
      181 tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac
      241 tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca
      301 tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat
      361 gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga
      421 tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc
      481 attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg accgctactc
      541 ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg
      601 cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga
      661 atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat
      721 tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt
      781 tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac
      841 tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac tattcaatct
      901 ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca
      961 agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt
     1021 caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc
     1081 ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca
     1141 gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta
     1201 tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct
     1261 ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct
     1321 gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat
     1381 ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg
     1441 tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat
     1501 caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa
     1561 aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac
     1621 acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc tttatttaaa
     1681 aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag
     1741 gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt
     1801 atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc gctttgcact
     1861 tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc
     1921 aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga
     1981 aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta
     2041 tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca
     2101 aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt
     2161 ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac
     2221 gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga
     2281 tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa
     2341 attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt
     2401 cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg
     2461 gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg
     2521 aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa
     2581 gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc
     2641 ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc
     2701 ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga
     2761 actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat
     2821 caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac
     2881 aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat
     2941 ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt
     3001 aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa
     3061 gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat
     3121 gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa
     3181 aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat
     3241 ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa
     3301 gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt
     3361 ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg
     3421 actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa
     3481 gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca
     3541 acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg
     3601 atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa
     3661 tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt
     3721 ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact
     3781 gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat
     3841 gaagatacag cagttatttt cactaatggg tccattacag attcagctac tttaggagat
     3901 tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa
     3961 gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca
     4021 agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc
     4081 cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc
     4141 acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca accgcatttt
     4201 acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt
     4261 ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg
     4321 attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa
     4381 attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc
     4441 aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc
     4501 tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta
     4561 cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa
     4621 acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa
     4681 taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa
     4741 cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc ttttgaacac
     4801 cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc agccaagcta
     4861 aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca agcttttgaa
     4921 caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag aaaaattttg
     4981 tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa ctgatccctt
     5041 ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat tttttcaacg
     5101 tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat gattaccatt
     5161 acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta tagaatgttt
     5221 catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa aatgaaatta
     5281 tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct ccaatgaaag
     5341 cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat ccaaaaatat
     5401 tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc tccgatctgc
     5461 atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga cacgaaccaa
     5521 tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg caacgtgtgg
     5581 ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac atggaaagcc
     5641 aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt ggcaagttga
     5701 acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca gactctactt
     5761 catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta tcagatattt
     5821 tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat acagttgtga
     5881 ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg gctggagcag
     5941 agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct aattacaatg
     6001 gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg tatgaactgg
     6061 ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta gagcctgtca
     6121 aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca ttacagatgg
     6181 catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat atgactccag
     6241 cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag catggacagg
     6301 caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga ctattcgatc
     6361 ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca tatgagatct
     6421 ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt cagtaaaaaa
     6481 tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc gaaataggaa
     6541 aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa aatcgttaca
     6601 cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt ggaagccttg
     6661 cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct ggtgctattt
     6721 tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac gacgaaaaaa
     6781 aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa tctcatgcct
     6841 cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac tttcttcctt
     6901 gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat ctgtaattaa
     6961 gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta atatccgctt
     7021 ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat ttggtgtcag
     7081 atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga tggtttcttt
     7141 ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc cagtacgaat
     7201 actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat tcgcgctttg
     7261 ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca atgtttaaac
     7321 ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa ttagcaatga
     7381 caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg ttttttgatt
     7441 aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt gacgaacggt
     7501 agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc tgcaatactt
     7561 tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat aacggtagct
     7621 gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac cattggtgtc
     7681 aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa catcgttggt
     7741 tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat atatggtttg
     7801 tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc tgttcctcct
     7861 aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct atgtccagct
     7921 gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat caaaggatcg
     7981 accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa tgcaagcagt
     8041 aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac tgtatagaaa
     8101 gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa agcaaacgca
     8161 gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca
     8221 ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa gatttctgtt
     8281 gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc taatgctccg
     8341 cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc ttttcggtag
     8401 ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta
     8461 ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat tgagttcatc
     8521 aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc tcctaagatc
     8581 attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca
     8641 atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt atccataaat
     8701 cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat caatgatctc
     8761 ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt ggaccgtcaa
     8821 aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg
     8881 atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta aagtgaaatc
     8941 accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc cgccaaacat
     9001 gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta tcctccgata
     9061 atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca ccattcatca
     9121 tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct agcttttcag
     9181 caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct acatttttag
     9241 attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct tccattcctt
     9301 ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt ccagctgaac
     9361 atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata cttacacttg
     9421 tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata tcatcacatt
     9481 taaggtctat accaagttgc atacacacta aaaaagaaag cgcacactaa acacacaaat
     9541 taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt
     9601 cacaactttt ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat aagtccaacc
     9661 aagttaaaaa cataagatgt tgtttttact ttgctattct tattacttga tgcaggacag
     9721 ctttttcaca acctctcaat aaacggtgtt caatcagctg accctgggta ttagctcaaa
     9781 acgctggatt cacaacctct tttatttcaa ataaccagta aatacaatga acatctacaa
     9841 aaaacttcta tttatttata gatttcgtca ggcaaatcca attctctctt atcaatcaaa
     9901 gtctgatacc acgaagcaga ttttttcaat gttctgcttc gttcatcgtt caattcgatt
     9961 cgaactaatc catatcgatt cttaaatgca ttcattggtg acacacagtc agtgaatgcc
    10021 cataacatat agccttcgca atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa
    10081 tgctgtccga taaattcgat acggtaatca tcttgcacga ttcctttctc atccatatac
    10141 tgttcttcat attctcttcc cataccgttt tctgttatca gccaagggat attatgataa
    10201 ttttctttta gatacatggc tatatcatac atgattttcg gatagatttc ccatcccctg
    10261 gaattattca ttttcttccc aggtaaatca aagacttcat aatatcgttc aggatggaaa
    10321 ggtgtctctt cattccattg ataacgaggg gcgcgaacgc gtttaggata atattgattg
    10381 attccaagga aatctactgt atgatgctga atcgtttcta aatcctctgt tgcaggatcg
    10441 aaataaatcg attgtttttg acacaactcc aggaactctt ctgtgtagta tccttttacc
    10501 atcggatcaa aaaatactcg attataaaac aaatcataca tataagcagc tttttggtct
    10561 tctggagaat tggatcgagc atagaccatt tcaggattta aaatacagcc aatccttcca
    10621 ccataatttc cgctgcggta aatctctact gctttagccg ttgctaaaac tttatgataa
    10681 ttccacatca tccacttttt tgtgttttgt tcatgcggcc agcgaacagc atccaaatag
    10741 caacgcgttt ggggaacgat tggttcgttg aatgtaaacc agttttttac cctatcaccg
    10801 taacgatcaa aggcaatttg agcatactta gcataaaaat caactacttt tcttgaagac
    10861 cagccatcat atttttccaa aagaaaagct gggacttcat aatgttccaa acaaatcatc
    10921 ggttctacgt ctgcttcgat caaagagtcg atcatttcac ttatatgctg agcatattct
    10981 tcatctacta tacagttctc ataatcagta aaaaaacgag cccaattgat agaagtccga
    11041 taatgtgtca gatggatgga tttcatcaga tccacatcct gctggtattg ttccataaaa
    11101 ttagttgcta cggctgggcc atacccatta tgccaaacaa aatgttcatt tttataccag
    11161 ctatctataa acgaatcctg tccttctttt tttcctttcc aaccttccgt ttgccaagca
    11221 gaagatgcag caccaagaat aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt
    11281 tttttagatc ccatcatttt ccacacgatt tgctactttt acaaatggca gatagatcaa
    11341 aatagaaaca ataatacaaa caagttgcgt aataactgca ccgatatttc ctgctgttgc
    11401 cagataagca ttgacgatcg gcggcattgg ccaagggacc attacaactg ctttcgccgc
    11461 aaatcctaaa gcggttgcaa aataaccaat cgttccagtg ataagaggcg ttaagataaa
    11521 cggaatagct aaaattggat tcagcatgat cggcatacca aaaataactg gttcattgat
    11581 gttgaagatc ccaggagcaa gggataactt ggtgatttgt ttcatatctt cccgttttcc
    11641 aactaacaat actgcaatca gtaaaccgat cgtgactcca gacccgccca tactcatata
    11701 catatcccaa aatggcatag taataatgtt cggtacttct tttccagctt caaaagcttc
    11761 cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt atcggcttca ccatttgatt
    11821 tccatggata ccaatcaccc aaaagacttg cgcaataaac atcaatagca aaatacccgg
    11881 taacccttgt acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag
    11941 gtacattcct gtaagttctt tgatgataaa tcccgcagcc gcgatacccg atacagtcag
    12001 aatcgtcggg aataaagcag aaaaactagc agaaacattc gttggcacgg tatcaggcat
    12061 tctcagctgt agtttcttct gtttcctcaa ccagcaataa agttcaatag aaaggatgga
    12121 gaccaacatt cccaaaaata accctttcgt atctgtatat tgtttagcca atacgttttc
    12181 tactataaca gtctgatttt cattcactaa ttcataaaca gttggattaa cagataaata
    12241 agacatcact gcgatgatac cagggaatac accatccact ttattcagct tggctaattc
    12301 gatccctatc agaaaaacag catagatggt caagaaactt agtgtgacat agctgatggc
    12361 agatgcgata ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt
    12421 ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg
    12481 aatcaatgca gcaaatgcgt tttttatcac catgatatac cttaaattat tgattctcgt
    12541 tgccacgttc cctaaaacat tggctaattt atccataaaa ctcattccag atcccctcat
    12601 tcctgcttct tctccttgac gtcatctctt tttgtctatt agtcagcgct tacatttcga
    12661 tgaacagaaa ctatttactc ctctttttta ccaaatataa aacaaaaatt catctgatgt
    12721 atttatcata aaatacatca gatgaactgt caattagttt ctttttctcc tattattttc
    12781 tgtaattctc tccgatttat tgccatatgg acagtcattg catcaaacgc agcaagtgca
    12841 ttccgccttt cgatggcctg aacgatctct gtatgaatat cgatcgtctc gattttcaga
    12901 tgctggatat cataattttt attaaacaaa gcaattgatt cattaatgat tgggataata
    12961 tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc gatgtctaat
    13021 tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc
    13081 atttggacga tttgattctt cgttgcatat tccgctgcca aacctgccat cttaggctcc
    13141 aataaatatc tcagttcaaa taagtctcgt atcatccgat cctgatcttc aatcaaagaa
    13201 aaaccgagtg ggtcattggc aatccctcgc ttatcactga caaatgtccc cgctccttgc
    13261 cgaacttcaa ggatattcct ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc
    13321 cccacctcta aataatcgga caattcatat tcattcggca gtttatctcc tacttgcaac
    13381 tgcatttcct ttaaatatct aaggatcttg tcagtagtta cttctactag ggttttattt
    13441 tcttccaaac cacatacctc cttttcatct gatgtatttt ttcgtaatta ttataacacg
    13501 tttgaatcat ctgatgattt tttgtataca aaattgatta atccgttcat agacatacaa
    13561 acgaagaaga tatttaccat aaaaaagact aggataaaat tcactcctag ccttcgatca
    13621 tttttatttt gaacgacaaa aaatatgatg ttcaataaca gacgatcttc attcgattct
    13681 tgatgtcatc ttttgttttt tttaatatat ggataaaaag tttgattaca tcgtttctat
    13741 tctagaattt ggtcgattct caattcttct tctggtgaga aatctaaatt atccatcatt
    13801 cgaacacttt cttgtaactg acttaaccga cttgctccga ccagtacact tgtaacagat
    13861 ttttgtcgca aattccatgc aagggccatt tgcgctaatg attgtcctcg tgatacggcg
    13921 attgtttgca gtgctttgat tttttccaga gttgatccaa cttgttcttc tgaaaggaat
    13981 ggtatttcct tacgatgtgc tctagaatct tcaggaatac catgtagata acgatttgtc
    14041 agcaaccctt gagcaagcgg actgaacgtg attgccccta gcccttcttc ttctaatact
    14101 ggagatagat cgtcttctag tagtgcacga gaaaacatat tgtagcgcat ctgatgaata
    14161 atgaacggtg cttcctttct ttttaaaatt tcagtcattt tttttgtatc ttctccatta
    14221 tagttggata taccaatata taatgctttt ccttgtctta caagttgcat caaagcttct
    14281 gctgtttctt ccaaaggtgt atcagggtct gggcgatgat gatagaagat gtcaacataa
    14341 tctagcccca ttctttggag actttggtca caacttgaaa tgatggattt acgagagccc
    14401 cattccccat atggcccttc ccacatatga taacctgctt tgctagaaat aatcagttca
    14461 tcacggtagg cttgaaaatc ttctctaaaa atacgcccaa agtttttctc tgcagctcca
    14521 gcagggcctc cataattatt cgcaaggtcg aaatgggtaa ttcccatgtc gaatgcgcct
    14581 cttaaaatct ctctttgatt atggatagga tcataatcac caaaattttg ccataatccg
    14641 agagataaaa tcggtaattt caaacctgaa tgaccggatt tacgatattc cattttttca
    14701 taacgtgcac tatctgcaat atacaatttt tctcctcctc atcaaatctt tttaaaacgc
    14761 tttctttttt ctatttttag tttactacat tttaaagaaa aggagttaat caaaatgatt
    14821 ttttcgtata tttttttgcc atttgttttt tactcgttga tagatcagaa attgattacg
    14881 tgcttgctgg atttttttat aacgtttcgg ttgttttttg taaaattgct gatgatagtt
    14941 ttctgctggc caaaacgtac cggctggttc gatttttgtg acgattggtt ggtggaaagt
    15001 tcctgaactg tctagtttct gttttgattg ttccgctaat tctttttgtc gttcgacttg
    15061 ataaaaaatc actggacgat attgcttccc tctatcttga aattgtccaa atgtatctgt
    15121 gggatcagta atttgccaat agattgtcag caactcgcta taacttattt ctcttgtgtc
    15181 ataaatgatc tccacagctt ccacatgacc tgtgtaacca ccacttactt gatcataatt
    15241 tggtttttca acatgtccgc ccgtgtatcc tgacaaaaca gataggatcc cttttttcga
    15301 ttcaaacggc tcgaccatac accagaaaca tccccctgca aatactgcac gttcttgata
    15361 gtcagcatct tgcatggcat gcttggaaaa gtcatatttt tgctccttat caaatccttc
    15421 cgtaatcaat aaataaaagt ccattacatc aggcgtcaga ttcattcgga tagctaatgg
    15481 acgtagtgtt gcttctagtt ttgataactg ttctttaacg gatatatttt ctcttatgtc
    15541 attctttgtc tgaatcaata cttttcgttc ccaatctcgt gtattcggat tcaaaatcag
    15601 attataaagt gtttgaatga atatttgttc ctcatccata gattctcctc ctttccgaag
    15661 aagacaagct agtgacttta gctgtaattt atcataaagt gaggaaataa gcgattactt
    15721 catcccccta cagatttttc gtataaaaaa gagagaatcc ttatcgacag attctccttt
    15781 ttaatcaatt tattttataa atgaaaagtt aatttacctt ttatggaaaa ctattttaaa
    15841 aaataaacat aaatatgcag ttaatgtaac aagataagaa ataatagtcg ttactgtata
    15901 acttgtgctt actttaatat cagcaactcc tttgactccg cttatttgta ccaatccatt
    15961 agcattcacc tgctccttta caggttgatg atttatttct acattcgttc ctaaataagc
    16021 atatacagga agtgtgatta cttggctctt ctcattagtt actctatata tgatagaact
    16081 accagttgtt ttataaggaa ggtttaccca ttgactgcca atctttgctt cgtgttttat
    16141 tatatgctcc ttattctcca atgaagcgat aggagaataa tcatataaaa aataatccat
    16201 tcctttagtg gtatacgcct ctactgtttc tttgtttaca acaatatgtc ccggctgaga
    16261 aattattttt tgattgaaat tttttttact agaaacagtt aaagtgatta ataacaaaac
    16321 agtcataaat aaataaaata gttctcttgt cttattgtat cttctatttt tgaaaaatat
    16381 catcgttaat atcagagaag agaatattat ttgcaagctt aagatccgcc aaggaaattg
    16441 taacaatttg gctggcgtat tttgcagaag ataccatggc accaaactag ttgtagctag
    16501 ccaagtcaaa cacgtaccat aataaatctt tttttctatg tttgttaatt ttttaaataa
    16561 aatcagggga atacataaag ataacagacc aactagtccc attgagtaag tagttaagtc
    16621 gttgttcata gcttcagtca gactatcgaa cacattagat gcatgagcat ataaatttgt
    16681 cacgctaggt ttgttgattt tttggtagag catttgttca aacatcggat accaaaaagc
    16741 actcgtcaga accaatgtca ttcctgccgc actaaataat tttttaaata tagcaataaa
    16801 atctgcattc ttgaaacctt tagaaaccaa acgaataaaa ataaaaatga taattaatgc
    16861 tgtactcatt aatgttgata gtacatggct gtacactagc aaactcatac tcaatgctag
    16921 ctttacagac ggatactttt cttcttttcc agagatgatt tgataagcat aaaataatac
    16981 agggataaca agtgttattg cgatagcttc ggcaattgcg gaacgatagt agatgtctac
    17041 tgtccgataa gcagagaaaa tatagagaca actaaataaa aatgccgctt ttttttgttt
    17101 taaaaaccgc tcgccataat gataagctac gagtgttgta cacaagttca ataaccaaac
    17161 gtaaataata tagcttacaa tcaaattttt tgtcatccaa tataacatta cagctggaaa
    17221 aaaagtcaga aagggataaa aataatttac accatatcca gtataattga atgtctcata
    17281 attaatcgga ctggtaaaaa tagtcgataa ccccttgatt cgagataagt gaaaagaaag
    17341 atctccagtg gtataaatac tctgtgtatt tttgaaaaag atgaaagaat atatactact
    17401 aactataatt aatccactca atattactac ttttttattt attttcattc ttcttgttta
    17461 tctccttttt agtcacatat cgagaatctt tcaacttatt aggtgcaaat cgtttaaaac
    17521 ttgaatacac cactaaacaa taagtcagaa tactgatcca taaagccatg agagcagttg
    17581 gagtatattt tgttgtaatt tctatcttat tttctccagc atttacatcc acattcacta
    17641 agccatgttt tttataagag tcatttacga ctttaccgtt tacttttacc tgtgtaccta
    17701 aataagcata tactggtact gtcacttttt gctttttgtt gcttgccact gtgtaagtaa
    17761 tgttattatc tgcagcttta tacttccctt tttcccacga ttctcctact tttacttcat
    17821 gtttttctaa gtgaggcctt tcttttatcg cttctgttgg ggcgtaatcg tataatcctc
    17881 ccatatctga tgttgtataa cgtgggagtg tttcttcatt gataacaata tgaccgtgtt
    17941 ttgattggat aacttttgcg taattttctt ttgctgctat agacgtgacg agtagaaaca
    18001 ccacagtagc gccaatgatt aaccatgaat attttttatt catagtcttt ttcataaaaa
    18061 caagcgttat gatcattgaa gaaaaaaaga tttggatacc taagatacgc caaggaaact
    18121 ggagtaagct tatcggtgtt ttttgaaaca aaaaccatgg aaataaatta gttacgcata
    18181 cccatgtaga aactacgcca acatagatga ttttttcttt tttctccatt tctttcagta
    18241 tgaataaagg aaaaatcaac gcaaccaatc ctaataaccc catagtgtat gttgtaatat
    18301 cgttatttaa cgcttgaatg atactatctg aaatagaaag tgcttctagc tgcaaatctg
    18361 ttttgaaggg actgtgtatt tcttgataat tcatctgctg caacatagga taccaaaaat
    18421 agctagttaa tgcacaagtc aaaaaaacag cctttactaa attcacgacc acaaatttta
    18481 tatcgttttt tatttgcttg cttaaaaaca aacgaactat aatcatacaa gcaatcaatg
    18541 cactacacaa tagtgtagtt aagacatgag tatacacaag tagacttata ctgacagata
    18601 aagcgatgac ttttttttct ctattgaata tcaatccata agcataatat aaaacagctg
    18661 ggataatcgt aagagcgatt gcttctgcaa ttgcggatcg ataatagata tcaactgttc
    18721 gataagttgc aaaagtatat aaacaactaa ataaaaaagc agcatctatc tttttaaaaa
    18781 acttttctcc ataataaaaa ctaatcagaa tcgtacaaat atttaataac caaatatata
    18841 gaacataact taatattaag ttatttgaaa ttccataaaa aacgactgca ggaattattg
    18901 taagaaaagg ataaaaatag ttcagtccat ctccataatt gttaaatgtt gtatagttga
    18961 ttggcccttc aaaaatagaa gataaccctt tgattcgtga aagatgaaaa gataaatcac
    19021 ctgttgtata aatttcttga gcatttctca taaaatttac tgaatataaa gtactaagta
    19081 agataagaac tactaaaaga aatgcttttt tacttatttt catagatttg ttgttctcct
    19141 tttttaattc tattttcagt atactagaat tgtctttttt taataagttt tgcgtt
//
LOCUS       DAYYFD010000056        18562 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000056 DAYYFD010000000
VERSION     DAYYFD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.056"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..804
                     /locus_tag="ACHA25_001826"
     CDS             157..804
                     /locus_tag="ACHA25_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8763117.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    933..1149
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1197..3842
                     /locus_tag="ACHA25_001827"
     CDS             1197..3842
                     /locus_tag="ACHA25_001827"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8763118.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4152..5465
                     /locus_tag="ACHA25_001828"
     CDS             4152..5465
                     /locus_tag="ACHA25_001828"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8763119.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTEAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5455..6108
                     /locus_tag="ACHA25_001829"
     CDS             5455..6108
                     /locus_tag="ACHA25_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8763120.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6163..6855
                     /gene="radC"
                     /locus_tag="ACHA25_001830"
     CDS             6163..6855
                     /gene="radC"
                     /locus_tag="ACHA25_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8763121.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7005..7205
                     /locus_tag="ACHA25_001831"
     CDS             7005..7205
                     /locus_tag="ACHA25_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8763122.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7459..9138)
                     /locus_tag="ACHA25_001832"
     CDS             complement(7459..9138)
                     /locus_tag="ACHA25_001832"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8763123.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9140..9352)
                     /locus_tag="ACHA25_001833"
     CDS             complement(9140..9352)
                     /locus_tag="ACHA25_001833"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8763124.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9745..11475
                     /gene="efrA"
                     /locus_tag="ACHA25_001834"
     CDS             9745..11475
                     /gene="efrA"
                     /locus_tag="ACHA25_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8763125.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11472..13232
                     /gene="efrB"
                     /locus_tag="ACHA25_001835"
     CDS             11472..13232
                     /gene="efrB"
                     /locus_tag="ACHA25_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8763126.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13327..13524
                     /locus_tag="ACHA25_001836"
     CDS             13327..13524
                     /locus_tag="ACHA25_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763127.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13677..14162
                     /locus_tag="ACHA25_001837"
     CDS             13677..14162
                     /locus_tag="ACHA25_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8763128.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14266..14859)
                     /gene="clpP"
                     /locus_tag="ACHA25_001838"
     CDS             complement(14266..14859)
                     /gene="clpP"
                     /locus_tag="ACHA25_001838"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8763129.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15039..15566
                     /locus_tag="ACHA25_001839"
     CDS             15039..15566
                     /locus_tag="ACHA25_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763130.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15657..16394
                     /locus_tag="ACHA25_001840"
     CDS             15657..16394
                     /locus_tag="ACHA25_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8763131.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16398..17315
                     /gene="miaA"
                     /locus_tag="ACHA25_001841"
     CDS             16398..17315
                     /gene="miaA"
                     /locus_tag="ACHA25_001841"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8763132.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17317..18546
                     /gene="hflX"
                     /locus_tag="ACHA25_001842"
     CDS             17317..18546
                     /gene="hflX"
                     /locus_tag="ACHA25_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8763133.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5997 a         3211 c         4033 g         5321 t
ORIGIN      
        1 catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat
       61 ttgtctcaca cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac
      121 actaaatatg tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg
      181 ccaaaagcaa ctgcgaaaag attaccttta tatttacgct acttgaagtt gctggatgac
      241 tcaggtataa gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca
      301 acgatccgca gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat
      361 gtcccttttc ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc
      421 gcattagtag gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat
      481 gaaaatctaa atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt
      541 gatggagaaa tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg
      601 acagtagcaa tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc
      661 aaaggaaatg taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat
      721 gtcaatgttc aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag
      781 aattactcag aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact
      841 cgtcgtaatt ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt
      901 gttgactaaa aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact
      961 ggcacattca ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga
     1021 aggtagcttg ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac
     1081 tcacttgaga ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac
     1141 gtcctagaat acgaaagtat tttaggacgt tttttagttt aaaggaggaa aacagcatga
     1201 cggaagaaaa taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc
     1261 agcattggtt agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt
     1321 caatcgtaat cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata
     1381 cgaccttgca agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc
     1441 tgcctggtat ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag
     1501 agcaagggat ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat
     1561 ggaaagaaga gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag
     1621 attacagtag ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt
     1681 ttgttacctt atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc
     1741 ctcaagcacg aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct
     1801 tttatcatat gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc
     1861 gtccagaaac gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc
     1921 aagcgctcat cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg
     1981 cagatgaata tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg
     2041 atccaaacga ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg
     2101 atgatggtac gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc
     2161 gtaaagcaat cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga
     2221 tccacagtgt gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc
     2281 aatggtttgt aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc
     2341 aagcagtgga attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg
     2401 tccatgattg ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc
     2461 acaaagaaac aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg
     2521 tacaagatcc tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa
     2581 tgggctggcc agatacggaa agcgaagatt ataagcgata cttccctact agcactttag
     2641 taaccggtta tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat
     2701 ttactggcga acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag
     2761 ggcggaagat gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt
     2821 atggagcaga tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc
     2881 gcttcagcta tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt
     2941 cacgtttcgt tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag
     3001 aaaaatcagt ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa
     3061 ctgagctatt tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct
     3121 gggatgattt ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg
     3181 ctgtttcaaa acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt
     3241 tattgcatcc aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg
     3301 gaaattctct agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag
     3361 cttctaaagg gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag
     3421 aagtgaatac accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa
     3481 tcgatcaatt tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt
     3541 tgacgatatc aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg
     3601 cagaaatctt cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag
     3661 aaaaagaact ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg
     3721 aacgttttgt ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag
     3781 attatttaga gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat
     3841 aagaatagaa aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac
     3901 atcgttacag gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca
     3961 taaattttgt cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg
     4021 gatcatgtat caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt
     4081 ctctttgtca tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg
     4141 gggaattgtt gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg
     4201 gttcacgtcc tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga
     4261 gaaaagtaaa aacgatccat atcgctggaa caaacggaaa aggttctact gaagcttatt
     4321 tgagaagcct attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg
     4381 aaacgttcaa tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc
     4441 agcttgtaaa aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg
     4501 gtattactga attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag
     4561 tggacgtagc gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg
     4621 taccaatgct gacgggaata acaacgattg gacttgatca taccgaaata cttggagata
     4681 caattgaaga aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa
     4741 caggaaatat tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg
     4801 caccgcttta tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt
     4861 ggggagaagt atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc
     4921 ttttagggca acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact
     4981 gtcaattgga aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc
     5041 gatggccagg aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc
     5101 ataatacgca tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc
     5161 aaatcaacat tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat
     5221 tgcagcaagt accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg
     5281 aattaagtga agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat
     5341 tcggattagc agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt
     5401 cactttattt catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag
     5461 tataatgcag tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac
     5521 gaagccacac aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta
     5581 aaattcttag gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa
     5641 tttggcaaaa caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt
     5701 ttttcgcaag gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa
     5761 gaacatcaga ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta
     5821 ttgaagaaga acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa
     5881 cgagcaaaac cagacccaga aattttcttg ttagctaatc agaagctagg tacagcgaag
     5941 aaagaaacat tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag
     6001 atcccggtag tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca
     6061 gctggggttg tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct
     6121 tttcgtatct tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc
     6181 aaagaagtac ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct
     6241 ttatcggatc aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg
     6301 atgtcgattg ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct
     6361 actttgcatg agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa
     6421 gccttgatcg aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt
     6481 cgtaccagct acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa
     6541 catttggttt gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc
     6601 atcggttcgc tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt
     6661 tattgtgcag caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tattccatcg
     6721 cagcaggata tgaattttac caaaaggata caaaagtgtg gagaaatgat ggggatcact
     6781 gtactggacc acttgatcat cggacgcaaa cgatatttta gtttgagaga agaaggaatg
     6841 atggaagaga agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg
     6901 taattttgtt tgggaataga agttgaaaca tctgttgcac ctctatcgta cacgagcaat
     6961 gttacaattt aaattagtgc ttttggcacg aggaggatat tattatggaa aacggaacag
     7021 taaaatggtt caacgctgaa aaaggatttg gatttatctc acgtgaagat ggaagcgacg
     7081 tattcgttca cttctcagct atccaaggtg acggattcaa aacattagaa gaaggtcaag
     7141 cagtgacttt cgacgttgaa gaatcagacc gcggcccaca agctgcaaac gttgtaaaag
     7201 cttaatttta tctgtaaatc aaattgatgg tagacatcaa tttttacgag agaacaaagc
     7261 gagtaacaaa aatacctgcc acaagagaca atttctcttg tggcaggtat ttttttgtat
     7321 gaaaaagtaa ataaatttaa gaggctatga aagagccacc ttgtatcaag gaataagaac
     7381 aaacaaaaat ggcttctcat gtttttcatt tgttttctct tatattcagg acaaggtgct
     7441 gatttcataa cctcttattt attcttcatc tggtgtcatt accattggca aaatcattgg
     7501 atgtctttct gtacgttcga ataagaaagg ctgaagcgcg ccagtcatcg cttctttcaa
     7561 tttatattct gaacagtcag gtccattcaa acattctctt aaagcgtgga ataagattcg
     7621 ctgagcttca ttgatcattt ctcctgattc tcgcatgtag acaaatccac gagagagaat
     7681 atctggacca gccaagattt cttttttctt gatatccact gtcgcgacag ctaatacaag
     7741 accttcttct gaaagcaggt gacggtcacg taaaacgaca ttaccaatat caccgatccc
     7801 gctaccatcg atatacacat ctccagccac aaaatgccca gcaggacgcg cagaatcgct
     7861 agttaacgct aaaacatcgc cattttccat gatgaagcag ttttctgcag gaacacctac
     7921 atcttgtgct agagaagcat gtactttcaa catgcggaat tctccgtgga caggaataaa
     7981 atattttggc ttcatcaaac gaagcattaa tttttgttct tcttgtccac cgtgaccgga
     8041 agtatggatg ttattgattt ttccgtgaat cacttctgca ccagcttcag acagcaaatt
     8101 gatcaaatga ttgacacttg ttgtattacc aggaataggt gaacttgaaa agatcactgt
     8161 atcgtctggc tggatttgga tttgacggtg agtaccattc gcgatgcggc tcaacgcggc
     8221 catcgcttct ccttgagaac ctgtacatag gatcatcgtt tcattggcag gcaagcgatt
     8281 gatttcgtgt gcatctacaa atgtgccgtc tggaactttg atatatccta aacgttgacc
     8341 gttgacgatg gcattttcca tgcttcgtcc gaaaacagcg attttacgac cagttgccac
     8401 agcggcatct gctgcttgct gcaaacggaa gatgttagaa gcaaagctgg caaaaatgat
     8461 acgtccttcg atttttgaaa agatcttcaa aatagaatgc ccaatcgttt tttctgattt
     8521 agtaaatgtt ggaatctcag catttgtact atcagataaa aggcacaaga ctccttcttc
     8581 cccgatacga gccattttat gcaagtttgc tggttcgcca acaggagtga aatcgaattt
     8641 gaagtctcca gtcgctacga tatttccaga aggtgttttc accacgatcc ctaatgcatc
     8701 tgggatacta tgggtcgttc tgaaaaagct gacagaagtc tttctaaaac ggatgatcgt
     8761 atcttcattg atctcatgga gctctgcatc tcgaagcaag ccatgctcat ctaatttgtt
     8821 tgtgatcaac gctaatgcga gaggaccagc atagatagga atatttgctt gtttcagcaa
     8881 gtagggtatt ccgccgatgt ggtcttcgtg cccgtgagta atcaccaagg cttttacttt
     8941 gtgtaagttt tgtatgatgt agctgtagtc gggaatgaca tagtcaattc caagtaaatc
     9001 gtcttccggg aatttgatcc cggcatcaat caaaatgatt tcatcttgga attgtacccc
     9061 atacgtattt ttcccgattt cacctaatcc accaatagca aagacgcctg tctcgttatt
     9121 tttgatggaa agtttcatat taaaactcca ctaatgagaa atcagcattt tctttttcgt
     9181 actctagatg acttccttct agaagttgaa cgaattcaat attgtatggg gtattttctt
     9241 ccacacgttg gcgagcgtct acatcgcttt ttgcctcaat gtaaagagat tttgtttctt
     9301 cccgtttagg gttgcgtact tttgtttctt gataataaac tttatagatc attgttttgt
     9361 ctcctttatc tgcgtaaagc agtgatttag ctagttgatt tttttagaca acacaaaacg
     9421 aggggacaag ccacctggtt tttcatatgc tttgactaga atactggatt cgagtcaaag
     9481 agacaagcct cgtttttgct tatctgttcg gatatttttt caaccgtacc ttctgtataa
     9541 aaaatataca acaattaatt gttattctag cataaaacag caaataagta tagaaatcaa
     9601 ctagattgaa agaaaaaatc aatgtttatc atcccgctat aatcataatg acttttttat
     9661 gagagaagtg ttctattaat aggctcgttg tgctatcatt tttagtaatg aaaatgtaac
     9721 aaaagattaa aaggagatga gaaagtgaaa ctaatgtggc attataccat gcgttataag
     9781 aaattcttgt ttttcaattt catatgcgta tttggtttta ttttgattga attaggactt
     9841 cctacgattt tagcacgtat gatcgatgta ggaatccgca atgatgattt tgattatgtg
     9901 aagcaacaag ggatatttat gatcgttatt actgtaatcg gtattatttt gaatatcttg
     9961 ttaggttatt ttggttcgag gattacgact aatatcgtag cagatattcg agatgatttg
    10021 ttcgaaaaga tccaaagtta ttcgcatcag gaatacgaaa atctaggcgt atcttcattg
    10081 atcactcgta caacaaacga tgcttatcag atcatgttgt tcctgcaaaa tattttacgt
    10141 atcggtttta tggcaccaat gatgttcatt gtcagtttgt acatggtcat gcgtacaagt
    10201 gtgacactca gtttgtatgt agtaggtgca ttacctttac tattattggc agtcgtagcg
    10261 attgctaagg tgtctgaacc gttatctaaa aagcaacaaa aaaacttaga taaaatcaac
    10321 agtatcttgc gggaaaatct ttcaggattg cgtgtgattc gtgcattcgt caatgaaaag
    10381 tttgaagaaa aacgttttgc tgtagtcaat gagaattatg cttctagttc aaaaagctta
    10441 ttccgtttga tggctgttgc acaaccgggt ttttatttct tatttaatat cgtaatggta
    10501 ttgatcatct ggagcggggc agtgcaaatc gatgcaggcg agcttcgtgt aggagatttg
    10561 attgcgttta tcgagtatat attccatgcc ttgttctcat tcatgttatt tgctagtgtg
    10621 ttcatgatgt atccaagagc agctgtttca gctggacgta tccaagaagc cctagatgcc
    10681 aaaccttcga ttgtagaaga tccaaatggt atatctgata caaaaactaa agggtatatc
    10741 gaatttcaaa atgtaacatt tgcttatcca ggccattcgc aagagccagt gatacgaaat
    10801 gtcagtttta cagcctctcc aggagagacc gttgctttta ttggaagtac cggtagcgga
    10861 aaatctacgt tgatccaatt gatccctcgc ttttatgatg tgagtgaagg aaagatcttg
    10921 atcgatggag tagacgtgag agattatcgg ctaagcaagt tgcgagataa gattgggttt
    10981 attccgcaaa aagcgctgct atttactgga acgattgcag ataatttgcg atacgggaaa
    11041 gaagatgcta cgcaagaaga aatggaacgt gcagctgaga ttgctcaagc ttcagaattc
    11101 atttctcgaa aaccagatgg atttaatgaa catttatcag aaggtggttc caatttttca
    11161 ggtggacaaa aacaacgatt ggcaatagcc agagccatca tacgccgtcc ggaaatctat
    11221 atttttgatg acagcttttc tgcattagat tatcaaacgg atgcgaagtt acgtgctcgt
    11281 ctgaaaaaag aaacgacaga atctactgta ttgatcgtag cacaacgtgt gggaacgatc
    11341 atgcatgcag ataagatcat cgtattaaat gaaggagaag tcgtcggtat ggggacgcat
    11401 cgggagttgt tggaaaaatg cccgatttat tatgatattg ccgcttctca attatcaaag
    11461 gaggaattag catgaaaaaa accttttctt ccttaaaacg tttagctcga tatatcagac
    11521 cgtatcgtga gacttttctt ttagtgatcc tatttacagt tctgacagtg gctttcaacg
    11581 tggctttgcc atatgttaca ggacttccta ctacagaaat cagccgtaat attgccaatg
    11641 gcgaatcaat caatttttct tatatattca aatgtctcat ttggatcaca gttgttggaa
    11701 tagggtattg tatttcgcaa ttactttctg gtgttttgat gacaaatgtc gttcaaagtg
    11761 ccatgcatga tttgagaaga gacatagaag aaaaaatcaa tcgtcttccg gtttcatatt
    11821 tcgataaaaa ccagcaagga aatattttgt ctcgtgtgac gaatgatgta gatgcagtca
    11881 gcggtgcttt acaacaagct tttattggta tcgtaaatgc gatattggga attacgttgg
    11941 ctgcagcaat gatgttttat atccagccga tgatggcgct gatctctatg atcatgattc
    12001 caacatcgat ctggatctca aaacgagtga tcaatgcttc gcagaaatat tttcaaagca
    12061 tgcagaattc tcttggtgag ctaaacggct acgtccaaga aaatatgaca ggttttagtg
    12121 tattgaaagt atatggacgc gaaaaagaaa cattggaagg attcaaaaaa gttaatcaca
    12181 gcttgaaata ctttgggttt cgtgcagcat tcatttctgg cttgatgatg cctctagttc
    12241 aattaactgc ctatgctact tatattggta tggctgttct aggaagcttt tatgttatta
    12301 ctggggcgat cgttgtcggt caactacaag cattcatcca atatatttgg caaatcagtc
    12361 agccaatggg gaacgtgaca caattgtctt cagttttgca aagtgcatca gcggcaacga
    12421 aacgtgtatt cgagatttta gatgagccag aagaaaaaga aaacaaagta gatgcttcac
    12481 tgccagaaac gattttagga gatgtgacat tcgatcatgt cgattttgca tatgatccga
    12541 agaaaccatt aatccaagat ttgaattttg aagtgaaagc tggtcaaacc gtagctgtgg
    12601 tcggtccgac cggtgccggc aagacgacat tgatcaatct attgatgcgt ttttatgacg
    12661 tagacaaagg agcaataagg attgatggaa tcgacaccaa atcaatgagt cgcagtgatg
    12721 tgcgttcgct atttggtatg gtcttgcaag atgcatggct ttatcaagga acgattgcag
    12781 ataatatccg cttcggaaag cttgatgcga cagattatga agtagttgat gctgcgaaaa
    12841 cagcaaacgt agatcatttt atccgaacga tgccagatgg atatgatatg gccatcaatt
    12901 ctgagggaga caatgtgtct ttaggacaaa aacagttgct gacgattgcc cgagcagtgg
    12961 tctccgatcc taaaatcttg attttagatg aagcaacaag ttcagtagac acccgtttag
    13021 aagcgttgat tcaaaaagca atggatcgcg tgatggaggg acggacaagt ttcgtcatcg
    13081 ctcaccgttt atcgacaatc cgtgatgcag atctgattct agtgatggat caaggtcaga
    13141 tcattgaaaa aggaacgcat gagagtttat tggcacaagg tggattttat gaaaaactgt
    13201 ataacagcca atttgctgag gaagcagaat aagtcatata agaataaaac agtttaaaac
    13261 ttattttttg ttctctattc ttgataatgg taaagtaaag ttatcaagaa aagtgaggac
    13321 acggagatgg aacagttttt aaatgaaaaa attactgaag ccaaaagaga agttgatcgt
    13381 ttaacgcacg tctgtcagac gaagcctttt gattcagctg atttttttga aaatgagttt
    13441 caaatcgaac gcttggcagc acaaatagaa gtgtacgaat taattttaaa aaaagtaata
    13501 gaagaaaata tagatgtaca ataaaaacag gtaaaacgtc agttaccaga tactgtaaat
    13561 agacatctgg cctgagcagg ttttacctgt tttttttctt atgaaataaa tatatgctat
    13621 gataccttta ttcaatagga ttagtttcta caaaaaatga aaggagaaaa ataaatatgg
    13681 aacggtttat agcatcaaat gccacagtga ttggtgatgt gacattgtca gaagatgtga
    13741 cgatctggta tcaagctgtt ttacgcgggg acagtaattg gataaaaata ggacaaagaa
    13801 cgaatatcca agatggtaca atcatccatg tcgatcatga tgcacctgta gatattgcgg
    13861 agaatgtgac ggtaggacat cagtgcatgc tacatggctg tacaatcgaa aaaggggcgc
    13921 tgatcgggat ggggaccacg atattgaatc atgcggttat tggtgagaat agtttgattg
    13981 gtgctggatc attagtaaca gaaggaaaag ttattcctcc taatgtcttg gcttttggtc
    14041 gcccagctaa agtgatccgt cctttgacaa aagaagaaat acaaaaaaac aaagaaaaca
    14101 tccagcatta tgtcgagata ggtcggatgc atgcgcaagg aaaattcaag gagcaaagat
    14161 aggcagtagc atccataaaa taaaaaagga tggtcagttt gagttgacca tcctttttta
    14221 tttggtttag ataagagagc ttaaaaaagc ttcctttgaa acagattaat ttaatgaaga
    14281 actgttttcc atgacttcat cgatcaatcc atatgctttt gcttgttcag ctgtcatata
    14341 gttgtcacga tctgtatctt tttcaatcac ttcaagcggt tgtccagtgc gttcagctaa
    14401 aattttgttt aagcgttcgc gtgtttgcaa aatatggcgt gccgcaattt cgatttcagt
    14461 tgcttgccct tgagcgccgc caagaggttg atggatcatg atctcagcat ttggtaaagc
    14521 aaatcgttta ccttttgtac cagctgtcag caagaagctt cccattgaag cagccatacc
    14581 cataacaatt gtttggacat ctgctttgac aaagttcatt gtgtcgtaaa tagccatacc
    14641 tgctgtaact gaaccaccag gagaattgat ataaagatag atatcttttt cagaatcttg
    14701 agcgtctaag aaaagcaatt gggcgatgat cgaattagca agatcatctg ttacttggcc
    14761 gcttaacatg atgatacggt ctttcaataa acgtgagtag atatcgtaag ctctttcacc
    14821 tcgtgaagat tgttcaatga ctgtaggaat taaattcata atagagttcc tccttgattt
    14881 cgtttagaaa tagtgtacca taatttagtt tctaatacct aaaatgtagt atacattttt
    14941 ggtcaaaaaa ggtcaaataa aaaacttagg caaattagtc gcataaaccc gctttatggt
    15001 atgataagca gagaaacaaa acaaggagga acaacagatt gatagttggt attactggcc
    15061 ttatcggagt gctgacaggt gctgtagttg tctatatcgg catgatactg caaatgcgtc
    15121 acttggaaaa taaagaacag cgtagacgtg aattggaatt gaaagtcaaa gaaatcgaga
    15181 cgctgaatca attaaataaa aagatcaacg agatcttgca aaaacggaca acgctgatgg
    15241 aagaatatgt cagctttgat gcatttgatg attgttatat cacgattgac gattttgcgt
    15301 atctccaatc attttcagcg caaaatagtt tttatctgcc aaattatatt ttagacgagc
    15361 tatttaagaa tatctcccat cgaaaagtga ttttatctcc agatgaaaca atgaagattg
    15421 gtggatatgc ttataaaggc ggacgcgtga ttttagagaa ttttacggat agtctgactg
    15481 aaatgatcaa tgaaaaaaaa gctcagttga agactatgac gcgccagccg attggtttct
    15541 tctcgaaaga tgatgtttgg agctgatatt ttgaagcatt gccgttgcac atgagagcgt
    15601 ggtaatgctt tctttttttc ttaatgatac aatagaactg aacggagtga agaaagatgt
    15661 tacaaatcta tgcccatcgc ggaagcagcg gcacccaccc agaaaatacg cttcctgcat
    15721 ttgcagaagc tgtacgagta ggtgcagatg gtatcgaact agatgtccat ctaagcaaag
    15781 atggttattt gatcgttatg cacgacgaag aagtggaccg gacgactaac ggtaaagggc
    15841 tgattcgaga aaaaacattg gaagaactca aaaaattgaa tgcagggggc aaattcagca
    15901 aagaatttcc agcagctaaa gttcctgaat tgagagaagt gttaggactg ctcctccaaa
    15961 aaaattaccg agggatgcta atgattgaaa ttaaaactga tcagtatgaa tatcccggta
    16021 tcgaagaaaa attagccgaa acattaaaaa gtagagattg gccttttcaa catgcatatt
    16081 gcagctttca tttggattct ttggaaaaaa tcgcattact ggaaccggag atacagctgg
    16141 acttattgat gagtacttct gataaaaaga tccaactagc agcagaaacc ccttatatag
    16201 aaggagtaca tccaaaaaat acttggttgt tcgaacatcc agtccaggca gctgattttc
    16261 caaaagcaat tcgtgcatgg acgattaacg atgaagaaga aatggccaaa tgtcaagaac
    16321 tcaaaataga tggaataata actgattttc cggagaaagc tcttcggtgg aaacaatcaa
    16381 cgaaaagtag gtaaaatatg gaaaaagtat tagtgatcgt cggtccgaca gcagtcggta
    16441 aaacagcgct aagtgtggaa ctcgcacaac tatttggagg agagatcatc agtggtgatt
    16501 cattgcaaat ctataaaaat ctggatatcg gtacagcaaa aatcaaagaa gaagagatga
    16561 ataatgttcc gcatcatttg attgatgtga tcgaacctac tcaacgttac tcagccgctg
    16621 attttcagca ttcaggtcga caattgatta ccgagattgc gaaccgaggg cacctaccta
    16681 tcatagctgg tggtacgggt ctctatatcc aatcccttct atatgattat cagttaggag
    16741 cgacagaaga agcgaatcct gagattagaa aaaagtatga aacgttagct gaaacaattg
    16801 gttcccaagc attatggaat cagttgaaag aaaaagaccc ggcagctgct gagaaaatcc
    16861 actggaacaa tcaacgcaaa gtgataagag ctctggaagt tttagagacg acaggacata
    16921 gtatcacggc acctaaagaa gagccaaagc aattgtatga ttacctcatg attggattag
    16981 atacgaagcg tagtattctt tatgaacgaa tcaatcaaag agtcgatttg atgctagcag
    17041 aaggtttagt agcagaagcg cgtaaggtgt atgagcttgg agaagtccag gccagtcaag
    17101 gtattggata taaagaattt ttcccttatt ttgaagggaa agaaacattg gaagaagcaa
    17161 cagaacaagt caaactacat tcgagaagat acgcgaaacg tcagttgacg tggtttcgta
    17221 accgtttgga tgcacagtgg tttgatctta taagtgagcc aaatcaaaaa agtcagattg
    17281 agcaaacaat caaaaaatgg ctggaggcgg aataaaatgg aagaaaaagt gatcatcgta
    17341 ggggtagaaa ccgaagcaaa ccagcgttac ttttcagagt caatggaaga gctagttcag
    17401 ctgacgaata ccgcatcagg agaagtcgta ttcacaatta cacaaaagag accacaagtg
    17461 gatcggcaaa cgattatcgg taaagggaaa ttacaagaat tagttcaaca agcggatgcg
    17521 catgaagcgg atttgatcat ctttaatcat gagctaactc cgagacagag ccaactgatt
    17581 accgatgctg ttggtcttcc ggtaattgat cgcgtgcagc ttatcttaga tatttttgcg
    17641 atgcgtgctc gttcgaaaga agggaagctg caggttgaac ttgctcagtt agaatacctg
    17701 ctgcctcgtt tagtagggca agggaaaaca ttatctcgtc ttggcggtgg gatcggaaca
    17761 agagggccag gtgaaacaaa gttagaaaca gaccgccgac atatacggaa taaaattttt
    17821 gctgttaaga aagaattgaa agaagtagaa gctcatcgag agcgcaatcg ccaaaaaaga
    17881 aaaaatagcg agatttttca gattggactt attggctaca cgaatgccgg gaagtcaact
    17941 atcttgaatc tattgacaca agcggatact tattcgaaag atcaattgtt tgcaacgtta
    18001 gatccattga ctaagagatg gcgttttgct gaaggatttg agattactgt aacggatacc
    18061 gtcgggttca tccaagacct gcctacgcaa ctgatcgatg ctttccattc tacattagaa
    18121 gaaagtcaaa atatggatct tttgctacat gtagtcgatg ctagttctcc agatcgtatt
    18181 ttacaggagc agactgtctt aaaattgatg gatgaactga atatgaaaga aatgccgata
    18241 ctgactgttt acaataaggc agaccagatc gacccagcga tgttcacgcc tacgctattc
    18301 ccaaatgtat tgatttctac tcagacgaaa gaaggtaaga tggcgttgat cgaagcaatc
    18361 aaacgtcagc tgatggaact aatgacccct tatacacttt ctgtgccaag caacgaagga
    18421 cagaaattaa gcgagttgag aagacaaaca atggttttgg acgaagcgta catggaagaa
    18481 acaaacgaat atgaagttaa agggtttgca aaaaccaatt caaaatggtt aagaaaaaac
    18541 caataaaatt ttattttttt aa
//
LOCUS       DAYYFD010000057        17743 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000057 DAYYFD010000000
VERSION     DAYYFD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.057"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..844
                     /locus_tag="ACHA25_001843"
     CDS             32..844
                     /locus_tag="ACHA25_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8763134.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            841..1641
                     /locus_tag="ACHA25_001844"
     CDS             841..1641
                     /locus_tag="ACHA25_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8763135.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1802..3070
                     /gene="rseP"
                     /locus_tag="ACHA25_001845"
     CDS             1802..3070
                     /gene="rseP"
                     /locus_tag="ACHA25_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8763136.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3138..4847
                     /locus_tag="ACHA25_001846"
     CDS             3138..4847
                     /locus_tag="ACHA25_001846"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8763137.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5059..9411
                     /locus_tag="ACHA25_001847"
     CDS             5059..9411
                     /locus_tag="ACHA25_001847"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8763138.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9555..10028
                     /gene="rimP"
                     /locus_tag="ACHA25_001848"
     CDS             9555..10028
                     /gene="rimP"
                     /locus_tag="ACHA25_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8763139.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10052..11227
                     /gene="nusA"
                     /locus_tag="ACHA25_001849"
     CDS             10052..11227
                     /gene="nusA"
                     /locus_tag="ACHA25_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8763140.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11249..11542
                     /locus_tag="ACHA25_001850"
     CDS             11249..11542
                     /locus_tag="ACHA25_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8763141.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11539..11850
                     /locus_tag="ACHA25_001851"
     CDS             11539..11850
                     /locus_tag="ACHA25_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8763142.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11863..14169
                     /gene="infB"
                     /locus_tag="ACHA25_001852"
     CDS             11863..14169
                     /gene="infB"
                     /locus_tag="ACHA25_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8763143.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14196..14543
                     /gene="rbfA"
                     /locus_tag="ACHA25_001853"
     CDS             14196..14543
                     /gene="rbfA"
                     /locus_tag="ACHA25_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8763144.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14651..15155)
                     /locus_tag="ACHA25_001854"
                     /pseudo
     CDS             complement(14651..15155)
                     /locus_tag="ACHA25_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15320..17683
                     /locus_tag="ACHA25_001855"
     CDS             15320..17683
                     /locus_tag="ACHA25_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM8763145.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6048 a         3148 c         3910 g         4637 t
ORIGIN      
        1 tgggaatctt ttaagtatag gaggaaaaag aatgttacgt ttttttccgc aaaaaaacaa
       61 atatattcaa gaaaaaagcg aatttcaatt taataccgat ggaccaattc ctcagcacat
      121 tgccatcatc atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc cacgagttgc
      181 tggccataaa gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat caaggcttgg
      241 agtgaaagtg ttgacgcttt atgccttttc gactgaaaac tggaagcgac caaaagatga
      301 agtaagtttt ctcatgcaac tacctgtcga cttctttgat acgttcgtac cagaattgat
      361 taaagaaaat gtcaaagttc atgtcatggg ttatgaaaac gtattgccag aacatacgca
      421 agatgctgta agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg tattgaattt
      481 tgctttgaat tatggaagta gagcagagat cgtgactgct gtccaagaaa ttgccgaaga
      541 agtagcaaaa ggagaaatac acgcagaaga aatcgatgat gaattgattg ctaagcattt
      601 aatgacaggt ttcttgccta aagagttaca agatcctgag ctgatgatcc gtacaagtgg
      661 agaagagcga attagtaatt ttctgttatg gcagatcgca tatagtgagc tgtattttac
      721 aaaagcgctt tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg cctcttatca
      781 aaatcgagac cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag gggatcagtc
      841 atgaaacaac gtgtgatcac agcagctgtg gcgctagtct tgtttattcc gattatatgg
      901 atcggaggca tagcggttga gcttacagca gcaatcttag cggtagtagg agtttacgag
      961 ctgttccgga tgaaaggatt gacactctta agttttgaag gagttttatc cgccgttggt
     1021 gcagtgttac ttgttttgcc aaaagaaaga tggtttttct ttcttccaga gaatgtaagt
     1081 acatttatgt tattttatct tatagttatg attcttttag gaacatcagt tatttctaaa
     1141 aatacgtata cgattgatga agcaggtttt cctgtgcttg tcagcttgta tgtgggaata
     1201 ggtttccaga attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat atttggtcta
     1261 tttattgttt gggcaactga tatcggagca tatatggttg gtcgacgtta tggtcagcat
     1321 aagttaagtc ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg tggtatcggt
     1381 agtgctgttg tagtcgcatt tctttattta ttgctatacc cagcaaaaga actattcaat
     1441 catggtatgt ttgtcatgct tctgatgacg attatttttt cagcagtcgg acaatttggt
     1501 gatttggtcg aatctgcaat caaacgtcat tatgatgtga aagactcggg aaatatctta
     1561 ccaggccatg gtggaatctt agaccgtttc gacagtttgc tgtttgtatt tcctatcatg
     1621 catttatttg gcattattta gtaagtatac ctgcgtttct ttgtgaattt cataaaagac
     1681 gcaggttttt atttttcatt atattgatac tattggtgtc tttggtatac tatggtaaaa
     1741 ttatagtgtt ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg agaaaaaagc
     1801 tatgaaaacg attctgacat ttatcatcgt ttttggtata ttagtgattg ttcatgagtt
     1861 tggtcatttc ttctttgcga aacgatcagg aatcctcgtc cgcgaatttg ctatcggtat
     1921 gggtccgaaa atttatgggc atcaagcaaa agacgggaca acatatacgc ttcgcctatt
     1981 acctattggc ggttatgttc gaatggcagg caatggggat gatgaaacag aaatggcacc
     2041 aggaatgcct ctttctcttt tactaaattc tgacggcatc gtagagaaga taaatttaag
     2101 taagaaaatc caattgacaa atgctatccc gatggaattg agccgttatg atttagaaga
     2161 tgagctgact atcactggtt atgtgaatgg agacgaaaca gaagtagtaa cctaccctgt
     2221 tgatcacgat gcgacgatta ttgaaaacga cggaacagaa atcagaattg cgccaaaaga
     2281 cgtacagttc caatcggcta aattgtggca gcgtatgctg accaactttg ctggacccat
     2341 gaataacttt attttggcaa tcgtgttatt catcattctg gcatttatgc aaggtggtgt
     2401 tcaagtgacg aacactaatc gcgtaggaga gatcatgcct aatggagctg cggcagaagc
     2461 aggcttaaaa gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc attcatggaa
     2521 tgatctgaca acagtcatta cgaaaaatcc aggtaaaaca ttagacttta agatcgaacg
     2581 agaaggtcag gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa atggcgaaaa
     2641 agttggccaa cttggtataa aagccccaat gaatacaggt tttatggata agatcattgg
     2701 agggacgcgt caagctttca gcggttcatt ggaaattttc aaagccttag gatcactatt
     2761 tacaggtttc agtttagata aattaggcgg acctgtcatg atgtaccaat tgtcttcaga
     2821 agcagcaaat caaggtgtaa caactgttat tagtttgatg gcgcttcttt caatgaatct
     2881 cggaatcgtc aatctgcttc cgattcctgc cttagatggc gggaaattag tcttgaatat
     2941 ttttgaaggg attcgcggga aaccattaag tcaagaaaaa gaaggaatat tgacattagc
     3001 tggctttgga tttttaatgt tgttgatggt gctagttaca tggaacgata ttcaacgatt
     3061 ctttttctag aaaaagttga caaatagggt ataataacaa acgataacta ttttgaggaa
     3121 ataaaggagt atatagaatg agacaatcaa aaatgttgat tccaacatta cgcgaagttc
     3181 caaatgatgc agaagtatta agccatcaaa tcttgctaag agctggatat atccgccaag
     3241 tatcagcggg tatttattca tatctgccat tagcaaatcg tgtattggaa aaactgaaac
     3301 gcattatgcg ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg cctgccatct
     3361 tgccagcaga actttgggaa gaatccgggc gttacgaaac atatggacca aacttgtatc
     3421 gtttcaaaga ccgcaatgat cgaaaaatga tccttggacc aactcacgaa gaaactttta
     3481 ctgatttgat gcgtaatgaa atcaattcat ataagaaatt acctttaaat ctttatcaaa
     3541 tccaacctaa atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc ggaagagagt
     3601 ttatcatgaa agatgcatat tctttccatg caacggaaga aagtttggac gagacatata
     3661 aagattacga aaaagcctat accgaagtat tcaagcgttg tggcttagaa tttcgctcga
     3721 tcatcggaga cggcggcgca atgggcggga aagactcaaa ggaattcatg gcgatctcag
     3781 aaatcgggga ggatacgatc tgttattcga ctgaaagtga ttatgctgca aacttggaga
     3841 tggctacaag ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa ttggagaaag
     3901 tagctacacc taacgtaaaa tcaattgacg aagtagcaga tttcttttct gttgaacctc
     3961 aaaaaatcat caagtctgta ttgttcattg cggatgaaca acctgtcctt gttttggtac
     4021 gtggagacca tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct gactttttag
     4081 aagaagcaac agaagaagaa gcacaaaaat atctaggtgc tgagtttggt tctgtcggac
     4141 cagtcggtgt ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa gatttagcga
     4201 atgccattac tggagcgaac gaaacaggat tccactataa aaatgtgaat ccaaaacgcg
     4261 atttcactgt gctttcttat gaagatcttc gttttgtaca agaaggagat ccttcaccag
     4321 acaacaacgg tgttcttgcc tttactcgag gaatcgaaat cggacatata ttcaaaatcg
     4381 gcactcgtta cagtgaatct atgggagcaa ctgtactaga cgaaaatgga cgagaaaaat
     4441 ttgtgattat gggctgttat ggtatcggtg taagtcgttt attatcagca atcgtagaac
     4501 aacattcaga tgaacaagga atcaattggc caaaaggaat tgcgccattt gatctgcata
     4561 tcgtccaaat gaatctaaaa gacgaatacc aaacaagcct gacgaatgaa ctagaagaag
     4621 cgatgacaaa agctggctat gaagtactgg ttgatgacag aaatgaacgc gcaggagtga
     4681 aattcgcgga ttctgatttg attggctgtc caatcagaat cactgtcggt aaaaaagcag
     4741 tggatggaat cgtagaagtg aaaatcaaga aaactggcga aatggttgaa gtacgtaaag
     4801 aagaattggc gaatacttta ccaatcctct tgaaccaagg cgaataataa gcgttaaaac
     4861 acagaaaata aaaatagctg aatcagctgt ttttgacaat ctgatgattc gcttttaaaa
     4921 ttggaactgc gatttgaatc gcagttccaa ttttttaatg taaaactcag aaaaaacgag
     4981 tatactaaaa ggtactgaaa aagaagagga taagatgcga tcaattgcat tgtttctcat
     5041 cagaaagggc gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa
     5101 atccaactag aggaagcaga aaaaaatcat ccactgctta ctgctggtga aatggatagt
     5161 gtagtggtcc atcgtaaatc gcgtttatgg gaatttacgc tacatttttc caaaattttg
     5221 cctattatgc tatatcggtc attgactcag catctgacta tcgcttttaa agatattgca
     5281 caagtcaaac tgaatatctt agcagatgac cagacattcg atgaacagct cttgcaagat
     5341 tactgggccc aagctttgga aaatcagcaa tgcgatacac cactagcaca gcaagtattg
     5401 aaaacgcagg tacctgtgat aaaagatcgt aaagtcatct tgccaatcga cagtacagga
     5461 gctatttctt atttgaagca acaatacttg ccattagtag aagagttgtt cgtcagttac
     5521 ggattcccaa aatttcgtat cgaaccagaa gtggatgagc agcaagcgga acgtgtattg
     5581 aaactttttg aagaacgcaa acaagaacag gcagaagcct ttatgaagca agcagctgaa
     5641 agtctagttg tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa
     5701 ggacccatcc agctaggacg aaatattcca gccgatgaac caacgacacc aatgatcagt
     5761 atcgttgaag aagaacgaag agtcacactt gaggggtacg tattcgacaa agaagtcaga
     5821 gaactacgtt caaaacgcaa gatcttgact ttgaaaatca cagattatac ttcatcattt
     5881 atcgtcaaaa aattctcgaa cggcgaaaaa gatgaacaag tatttgatgc gatcagtgtc
     5941 ggtagctggt taaaggtacg aggaagtatt caagaagata cgtttgtccg tgatttggtc
     6001 atgaatgcgc aagatatcat agaagtcaaa catacacctc gcaaagatta tgctcctgaa
     6061 ggggaaaaac gagtagagct tcatgttcat agcaatatga gtacgatgga tgcaacaaac
     6121 agcatctctg atcttgtggc acaagcagga aaatggggac accgagctat cgccattaca
     6181 gaccatggtg gtgcgcaagc ctttccggaa gcacatagtg ctggaaaaaa agcaggtgtg
     6241 aaaatcttat acggcgtgga agcgaatgtc gtagatgatg gtgtcccaat tgcttataat
     6301 gatgcacatg aagcattgag tgaagccaca tacgtcgtat ttgacgtgga aacgacagga
     6361 ttgtctgcag tatatgacac gatcatcgag ctagccgcag tcaaaatgta taaaggaaat
     6421 gtgatcgaaa gctttgatga atttatcgat ccaggacatc cattgtcacg aacaacggta
     6481 gatctgactg gtatcacgga tgaaatggtt cgagggtcaa agtcggaaga agaagtattg
     6541 cggatgttcc ttgaattttc aaaagataca attttggtag cccacaacgc ggcttttgat
     6601 atggggttct tgaatactag ctacgcaagg tatggtattc ctgaagcggc caatccagtc
     6661 attgatacgt tggaattagc tagatatctg tatcctcaat tcaaacgttt cggattaggt
     6721 gtgttatcta aaaagtttgg tgttagcttg gaacagcatc accgagcaat ctatgatgct
     6781 gaagcaactg gtcacttagc atggatcttt gtgaaagagg caatggacaa tcataacatg
     6841 ctttatcatg atcagctaaa tgagcatatc ggagaaggag attcttacaa aagggctcga
     6901 cctttccatg taacgatttt agcaaaaaat caggcaggac tgaaagattt gttcaaactg
     6961 atttcaatgt cgaatgtcga atacttcgaa cgcgtcccac gaataccacg ctcccaattg
     7021 aaaaagatgc gtgaaaatct attgatcggc tcagcttgtg ataagggaga aatattcgaa
     7081 gccatgatgc aaaaaggggt agaagaggca agaaaccgag caaaattcta tgattatatt
     7141 gaagttatgc caaaagcggt ctatgcgcct ttgatcgagc aagaattggt aaaaaatgaa
     7201 catgatttgg aagaaattat ccaaaatttg gtagaaatcg gaaaaagttt ggataaaatc
     7261 gttgttgcaa caggtaatgt acattatctg aacgaagaag atgccattta ccgaaaaatt
     7321 ttgatcaatt ctatgggcgg agcgaatccg ttgaatcgtc atagtttgcc ggatgtccat
     7381 ttccgaacaa ccgatgagat gctaactgca tttcactttt taggggaaga aacagcgaaa
     7441 gagatcgtag tagaaaatac gaataaaatc gcagatatct gcgaagaagt gattccagtc
     7501 aaagatgaac tttatacacc gaagattcca ggttcagaag atgaaatatc agaattgagt
     7561 tacacaaaag caaaacaaat gtacggagat ccgttacctg agatcattca aaaacgatta
     7621 gaaaaagagt tgaattctat taatggtaat ggcttctcgg ttatttatct gatcgcccaa
     7681 aaactagtgc ataaaagtaa tgaagatggc tatctagtag gttcacgtgg ctctgttggc
     7741 tctagctttg ttgcgacgat gacaggaatc actgaagtta atccattagc ccctcattat
     7801 tattgtccag agtgccaata ttccgagttt tttgaagatg gtacgtatgg ttctggattt
     7861 gacatgccag aaaaacaatg tccaaaatgt ggtgcacgct taaacaaaga cggtcatgat
     7921 attccatttg agacattttt aggattccat ggagacaaag tgcccgatat tgatttgaac
     7981 ttctcaggtg attatcaagc agaagcccat aactatacga aagtattgtt tggcgaagac
     8041 tatgtttacc gtgccggaac gattggtaca gtcgccgaca aaacagcata tggctatgta
     8101 aaaggatacg aaagggacaa taatctgcag tttcgaagtg cagaagtcga cagattagca
     8161 aaaggcgcta ctggagtcaa acggacaacc ggccagcatc caggggggat catcgttatt
     8221 cccgattata tggatgtcta tgattttacg ccgatccaat atccagcaga cgatcaaaat
     8281 tcagaatgga aaacgactca ttttgatttc cactcgattc acgataatgt attgaaatta
     8341 gatattctag ggcatgatga cccaactgtt attcggatgc ttcaagattt atcagggatc
     8401 gacccacaaa cgatccctac ggatgacccg gaagtgatgc ggatatttgc cggtcctgaa
     8461 gtacttggag taagtcagga acaaatttat tccaaaacag ggacattagg tattccagaa
     8521 ttcggcactc gttttgttcg agggatgtta gaagaaacgc atcctacaac ttttgcagaa
     8581 ctactgcaaa tttccggact atctcatggt acagacgtat ggttaggaaa tgcggaagaa
     8641 ttgattcgac gaggagatgc gacactggcg gaagtaatcg gctgtcgtga tgatattatg
     8701 gtttatttga tccatgctgg attagatagc gggatggcat ttaaaatcat ggaaactgtt
     8761 cgtaaaggac aatggaacaa gatccctgat gaattgcgag aaacctatct atcggcgatg
     8821 aaagaaaata atgtgcccga ctggtatatc gattcttgtt caaaaatcaa atatatgttt
     8881 ccgaaagccc atgctgcagc ttacgtattg atggctcttc gtgtcgctta tttcaaggtt
     8941 tatttcccta ttttgtatta ctgtgcatac ttttctgtac gtgcagacga ctttgatcta
     9001 gtctccatgt gcaaaggaaa agatgctgtg aaacaagcaa tgaaagaaat cacggacaag
     9061 ggattagatg cttccgtaaa agaaaagaat caactgacag tgctggagtt agccaatgaa
     9121 atgctggaac gaggattcaa atttggaatg attgatttgt ataaatcgga tgccgttaac
     9181 tttgttattg aaggtgatac gttgatcgca ccatttagag ctgtcccaag tttgggaacc
     9241 aacgtggcga aacaaatcgt tgaagcaaga aaagacggtc catttttatc caaagaagat
     9301 cttgccaccc gcggtaaagt ttcaaaaaca ttgatcgaat atatgaacga caatggcgta
     9361 ttgaaagatc taccagatga aaatcaatta tcgttattcg atatgctata agcatctgtt
     9421 ttcctgtctg cttgtaattt taagcagagt atggtatagt tcttataggt aatgatacta
     9481 actgtgagtg agcggatttt tcgctcactc tttttaatgg aattacgcac ttaattttta
     9541 aggaggcgaa acttttgagt agcgtcgttg aaacagtcac agaaatggtg acaccgattt
     9601 tagatgaaca aaattttgaa cttgtcgaag tcgaatttgt aaaagaagga aaaagctggt
     9661 tcttacgagt atttattgac aaagaaggcg gtatcgatat cgaagaatgc gcctttgtca
     9721 gtgagaaact gagtgaaaaa cttgatgcag cagatccaga tccgattcct caagcttatt
     9781 tcctggaggt atcctccccg ggagcagagc ggccattgaa aaaagaaagc gactatgaaa
     9841 aggcacttgg tgattatgtc catgtctcct tataccaacc agtagatggc gaaaaacaat
     9901 atgaaggatt tcttcaatca tttgatgctg aacagcttac attgaaaata cgcataaaaa
     9961 cacgagaaaa agaattgact tttgaccgaa aaaatattgc aaaagctcgc ttagctatcc
    10021 aattttaatc acggagagga atcagaaaaa aatgagtaaa gaaatgttaa atgcgttaga
    10081 cgctttagaa gctgaaaaag ggatctcaaa agaaatcgtg atcgatgctt tagaagcagc
    10141 attggtttct gcatacaaac gtcactatgg ccaagcacaa aacgtcgaag tggaatttga
    10201 tcagaaaaaa ggaaagatcc atgtctatgc ggtaaaagaa gtaacggaag aagtgatgga
    10261 ttcacagttg gaagtctcat tgaaagatgc acttttaatc aatccagcat atgagatcgg
    10321 tgatacgatc cgttttgaag taacaccaaa agattttgga cggatcgctg cgcaaacggc
    10381 taaacaagtt attttacagc gtgtacgtga agcagaaaga acgattattt ataacgaatt
    10441 cagtgcttat gaaaaagaca tcatgcaagg aatcgtcgaa cgccaggata aacgttatat
    10501 ttatgtcaat cttggtaaaa ttgaggcagt tctttctaaa caagatcaaa tgccaaatga
    10561 attttatcaa ccacatgatc gaatcaaagt ttatgtttcc cgagtagaaa atacgtctaa
    10621 aggtccgcaa gtatttgtta gccgcagcca tccagaccta ttgcgtcgct tatttgaaca
    10681 agaagtacca gaagtctatg atggaatcgt tgaaattgtc agcatcgccc gtgaagctgg
    10741 cgatcgttca aaagtggcag ttcgctcaaa tgatccaaat atcgatgcag tcggtacatg
    10801 tgtcgggcca aagggacaac gagttcaagc aatcgtcaat gaattaaaag gcgaaaacat
    10861 ggatatcgtc gaatggaatg aagatccagc tgtattcatc gcaaatgcat tgaatcctgc
    10921 acaagtcatg gatgtgatct tcgatgagac aaatccaaag gcctgcacag ttgtcgttcc
    10981 agactaccaa ttatcgctag caatcggtaa acgcggacaa aatgcacgtt tagctgctaa
    11041 attgactaac cataaaatcg atatcaaatc agaatctgac atgacggaat tttatgaaaa
    11101 tcaagaacaa caaaaagaaa cagaagaact gcatgatgaa gcaatcgttc agtcagattt
    11161 aacagacgat gaatatgaaa caattgcatt caacaatgaa acggtcgaag aaaaaccaga
    11221 agcttaactt tagtaaggag gcaaagagat gaaacaaagg aaaattcctt tacgcaaatc
    11281 cgtggtttca ggtgaaatga aacctaaaaa agagatggtc cgaatcaccc gttcaaaaga
    11341 aggggaagta tcgatcgatc caactggaaa aatgcctgga cgaggagcgt atgtctcact
    11401 tgagccggaa gaagtacaac aagcttggga taaaaaaatt ctcgatcgag tactcgaaac
    11461 aacattaaca gatgagtttt atcaagaact tcttgactat gtcactcacc aaaaagcacg
    11521 taaagagtta tttggaaaat gaatacagtt aatcggcaaa aagccatgaa tctcatcggt
    11581 ctagcgatgc gcgcgggaaa actaatcacg ggtgaagaac tgaccattgc tgaaatccgc
    11641 aaacaaaaag caaaattggt gtttgtggca accgatgcaa gtgagaatac aagaaaaaag
    11701 ataaaagata agagttcata ttatgaagtt ccttgctttg agctattttc tgaagcagaa
    11761 atcacgcaga tgatcggaaa acctcgcaag gtattcggaa taatggatgc cggatttgcg
    11821 aagaagacca aggagctaat tgaaggttag gaaggtgatt gcatgggcaa caaaagaatc
    11881 tatgaattag caaaagaact caaaaaatct agtaaagacg tcgtggaaaa agcacaacaa
    11941 cttggcatcg atgtgaaaaa ccatatgggt gcgattacta gcagtgatga gaagaaattg
    12001 caacaagcgt tcaaaaaccc aagcacgaaa caacaagctg cacaacaagc aagcagaaaa
    12061 ccagccaatc aacagccgaa cgatcaacgt aaaaaagaaa caacgaacaa tcaacaaaaa
    12121 aataaaaata accgcagtta tcaagatcgc ggtcaaggga gcggtcaagt gaatcaagga
    12181 aaaaaaccaa caacaaacca aaataatacg ggaaatacca gcagccaaaa tcgtcaagga
    12241 acgagccaag gcggcaatca aaaccgtact aacaataaca acaaaccaaa caataacaat
    12301 cgcggcaaat ttaataataa taaccgtaat cgttataata aaaaaggtaa aaaaggcaaa
    12361 cagcaacaat caactaaacc agctgtacca ccacgcaaat tccgtgaatt gccagatgta
    12421 ttagagtata ctgaaggtat gaacgtagca gatattgcga aaaaaattca tcgtgagcca
    12481 gcagaaatca ttaaaaaatt atttatgctt ggtgtcatgg tcaatcaaaa ccaagcactg
    12541 gataaagata cgattgaact gttagcgacg gactatggca tggaaccaca agaaaaagtc
    12601 caagttgaca ttgctgatat cgataaattc ttcgaggcag aagaagtcga tccagataaa
    12661 ttagtctcac gtccgccagt agtaacgatt atgggacacg tagaccacgg gaaaacaacc
    12721 ttattggata cattgcgcca ttcacgtgta acaagcggcg aagccggcgg aatcacacag
    12781 catatcggtg cctaccagat cgatattgat ggtaaaccaa tcactttctt ggatacacca
    12841 ggacatgcgg cctttacaag tatgcgtgca cgtggagcaa gtatcacaga tatcacgatc
    12901 ttagttgttg ctgcagatga tggcgtcatg ccacaaacag ttgaagcaat caaccatgcc
    12961 aaagctgcag gcgttccaat catcgtcgct gtaaataaaa ttgataaacc aggtgcaaat
    13021 cctcagcatg ttatgcaaga attaagtgag tatgaattaa ttcctgaagc atggggtgga
    13081 gaaacgattt ttgttgagat ctcagcgaaa ttcggccaaa acatcgaaga attgctggaa
    13141 atgatcctgt tagtcgcaga agtagaagat ttaaaagcag atccaacaca acgcgcaatc
    13201 ggaacagtga tcgaagcacg tcttgataaa ggtaaaggtc ctgtgtcaac cttacttgtc
    13261 caagaaggaa cacttcacgt tggtgaccca atcgttgtcg gtaatactta cggacgtgtc
    13321 cgtgtaatga caaacgatct tggccgtcgc gataaagaag caggacctgc aacgccagta
    13381 gaaatcactg gcttgaatga tgtacctcaa gcaggtgacc gtttcgtagt cttcgaagac
    13441 gaaaagacag cacgtgcagc gggtgaagaa cgcgcaaaac gtgcattatt ggaaaaccgt
    13501 gctgccacaa gccgtgtgac tttggacaac ttatttgaaa gcctgaaaga aggcgaattg
    13561 aaagaagtta acgtaatcat caaagcagat gtacaaggtt cagcagaagc actggctgca
    13621 tcattgaaga agattgatgt ggaaggtgtc cgtgtaaaaa tcgtccattc agctgtcgga
    13681 gcaatcaatg aaagtgatgt aaccttagca gcagccagca acgctattat catcggtttc
    13741 aacgttcgtc ctacaccgca ggcaaaagtg caagctgata ctgaagaagt cgatattcgt
    13801 cttcaccgta tcatctataa agccatcgaa gaaatcgaaa ctgcgatgaa agggatgcta
    13861 gaccctgaat ttgaagaaaa aatcaccggt caaatgatcg ttcgtgaaac cttcaaagtt
    13921 tctaaagttg gtacgattgc aggtgcctat gtaacagaag gatatattcg tagagatagc
    13981 ggtgtccgtg taatccgtga cggaatcgta atctatgaag gccaacttgc aagtctgaaa
    14041 cgattcaaag atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc gatgattgaa
    14101 aaattcaatg acatcaaagt agatgacgta attgaaggat ttatcatgga agaagtcaaa
    14161 aatgactaaa aactgataaa aggaggccaa tcattatggc taactaccgt gatcgccgag
    14221 tcggtcaaga aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc cgtgaccctc
    14281 gtgtggagaa cgtaacgatc acagatgtga aagttacggg ggacttacag caagcaacga
    14341 tctattacag cattttatct gacttagcat cagataagaa aaaagcacaa gatggattga
    14401 ataaagcatc aggattgatt cgtcgagagt tagggcacaa tctaagtatt tataagacac
    14461 ctgaattgtt ctttgaactt gatgaatcag ttgtctatgg gaaccatatc gatgaattgc
    14521 ttcgcgactt gaacaaagac taatcaaaaa atcccgcaaa tcctgtgaaa tcaggggttg
    14581 cgggattttt ttgttttcat taagacgtat tacctaagag ttccatgact ttagtctggt
    14641 agcgtgtcag ctaaccaaaa aagagaaaaa gaaaagtaaa aaattcgact ttcttttttc
    14701 tatagtatat aaatttttgc atatagataa agatcaagat accgataacg gaagctagat
    14761 tgtaatctat tttagtgaca gtcaggtata gccaagtaac tagaagatca gtggaacctg
    14821 caccataaaa gtttgagttt gtcggtccgt tgacagttaa aagaaaaatc acgttgaagt
    14881 tattgatatt tccgattacg ccgcttcgat ttggtcacga tccaaatttt ggataatccc
    14941 tgtagaaatt agcatagtag ctgggatacc aattcacata ttgacaaaga taacagagac
    15001 ttttacataa aacggatcaa tcaggaaagg aattgctcga tccagcccca gttttccaac
    15061 aatgtgttaa ctggcctagc gtcattcagc aaattatgca tgatgaggag cgaaatgaac
    15121 tgcggaacag ccattgtgat aacgacaagt tttttggatc aatgtaaaaa acgtcagcga
    15181 aaggaaaaat ttcgtggttt tttatacctg ttcatttggc tagatataaa atccaaacaa
    15241 atgaacagaa gtgcttatta ggtttcaatt gggagtatct gcgctagaat aagagtaata
    15301 atgaaaagga gtgttatgta tggattattc tagtaaagaa tactttgata aaatgacggc
    15361 ttggtggcgt gctgctaatt atctttctgt tgggcaactt tatcttaaag ataacccctt
    15421 actgagacgt acactgaaac cagaagatgt caaaaaacat ccaatcggtc attggggaac
    15481 gattcctggc caaaatttta tttatgtcca tttgaaccga gtcatcaata aatatgattt
    15541 gaacatgttt tatatcgaag ggccaggaca cggcggacaa gtcatggtat cgaatgctta
    15601 tttagatgga agttacacag agatctatcc agaagtaacg gaagatgaga caggaatgca
    15661 aaaattattc aagcgatttt catttcctgg aggcattgct tctcatgcag caccagaaac
    15721 accaggttct atccatgaag gcggagagct tggatactct ctttcacatg cagtcggagc
    15781 tgtattagac aatcctgaag taatctctgc tgtcgtgatt ggtgatggtg aagcagaaac
    15841 cggtccttta gctggaagct ggttctcaaa tgtctttatc aatccagtga tagatggagc
    15901 tgttttaccg atccttcatt taaatggtgc aaaaatagcc aacccaacga tcttggcacg
    15961 taaatcagat ggagaattag caaattactt taacggactt ggctgggagc ctttctttat
    16021 tgaaggaaat gatccagaaa aattaaatcc tgttatggct gaaaaaatgg atcaagcaat
    16081 cgagaaaatc aaatccatcc aaaaagaagc tcgcctgaag acagcaacag atgtcgtgat
    16141 gccaaaatgg ccagtattga tcgtacgcac accaaaagga tggaccggtc caaaagaatg
    16201 ggacggggaa ccaattgaag gaaccttccg agcacaccaa gtaccgattc ctgtagatca
    16261 agaacatatg gatcatgcag atgctttact ccgttggctg aaatcctatg aaccagaaaa
    16321 attatttgat gcacaaggac gtattttaga agaaatcaga gaaattgcgc ctactggtga
    16381 ccaaagaatg gcaaaaaatc ctatcaccaa tggtggaatc gatccaagac cgcttattat
    16441 gccagattgg aaaaaatata ctttgcaatt tgaaaaacca ggctccgtta aagcagaaga
    16501 tatgaccgaa cttggtaaat tcgttcgcga gatcattgag aaaaatccag aaaatttccg
    16561 tatatttgga ccagatgaaa caaaatcaaa ccgattgaat caagttttca aaacgacaaa
    16621 tcgccaatgg atggaaaaaa tcgaaccaga aaatgatgaa tggctttcgc catctggtcg
    16681 cgtcatcgac agtcaattat cggaacacca agatgaaggg ttcttagaag ggtacgttct
    16741 gactggacgg catggttttt ttgcaagtta cgaatcattc ttgcgagtgg ttgactctat
    16801 gttgactcag cacttcaaat ggatacggaa atcacatgac ctttcttggc gaaaagatta
    16861 tccttcgctg aatctcattg caagctcaac agtcttccag caagatcaca atggatatag
    16921 ccaccaagat ccaggaatat tgacacattt ggcagaaaag aaggcggagt tcatccgtga
    16981 atatctgcct gcggatgcaa atacattgct cgcagtcatg gataaagcat tccgctcgtc
    17041 agaaaaaatc aatttgatca tctcttccaa acatccgcgt gctcaatttt actcagcaga
    17101 agaagcagcc gtcttagtga atgaaggctt gaaaatcatt gattgggctt cgacagcaaa
    17161 agaggaggaa cctgaattgg tcattgctgc tgcaggaacg gaatctaact tagaagcatt
    17221 agcagctgta actttgctgc tggaagaatt cccaaaacta aaaattcggt tcatcaatgt
    17281 cgtagattta ttgaaattac gtcatccaag tcaagatccg cgtgggttga gcgatgaaga
    17341 atttgatcaa tattttacaa aagacaaacc gatcttattt gctttccacg gttatgaaac
    17401 attggtccgc acgattttct ttgatcgtca taaccatcat ttaatgattc atggctacaa
    17461 agaaaatgga gatatcacga caccatttga catgcgtgta gtaaatgaac ttgatcgtta
    17521 tcatttggca aaagacgcag ctttaaaaat caaaggaagt caagcagaag attttgcgga
    17581 aaaaatggat cagaaattac aagaacatca aaactatatt cgtgaaaatg gtattgatct
    17641 gccagaagta ttggattgga aatggaaaaa tcttgaccaa taaaatgata aaatcgatcc
    17701 cgcaaaacag aattttttac attgtattat ttgtcaaatt aag
//
LOCUS       DAYYFD010000058        16843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000058 DAYYFD010000000
VERSION     DAYYFD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.058"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..563)
                     /locus_tag="ACHA25_001856"
     CDS             complement(51..563)
                     /locus_tag="ACHA25_001856"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8763146.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(577..2874)
                     /gene="recJ"
                     /locus_tag="ACHA25_001857"
     CDS             complement(577..2874)
                     /gene="recJ"
                     /locus_tag="ACHA25_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8763147.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3016..3459)
                     /locus_tag="ACHA25_001858"
     CDS             complement(3016..3459)
                     /locus_tag="ACHA25_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8763148.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3460..4248)
                     /locus_tag="ACHA25_001859"
     CDS             complement(3460..4248)
                     /locus_tag="ACHA25_001859"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8763149.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4245..5186)
                     /gene="rnz"
                     /locus_tag="ACHA25_001860"
     CDS             complement(4245..5186)
                     /gene="rnz"
                     /locus_tag="ACHA25_001860"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8763150.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5363..5950)
                     /locus_tag="ACHA25_001861"
     CDS             complement(5363..5950)
                     /locus_tag="ACHA25_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8763151.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6171..7484)
                     /gene="obgE"
                     /locus_tag="ACHA25_001862"
     CDS             complement(6171..7484)
                     /gene="obgE"
                     /locus_tag="ACHA25_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8763152.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7772..8782
                     /gene="gap"
                     /locus_tag="ACHA25_001863"
     CDS             7772..8782
                     /gene="gap"
                     /locus_tag="ACHA25_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8763153.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8867..9802)
                     /locus_tag="ACHA25_001864"
     CDS             complement(8867..9802)
                     /locus_tag="ACHA25_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8763154.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            9988..10947
                     /locus_tag="ACHA25_001865"
     CDS             9988..10947
                     /locus_tag="ACHA25_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8763155.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11033..12127)
                     /locus_tag="ACHA25_001866"
     CDS             complement(11033..12127)
                     /locus_tag="ACHA25_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8763156.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12316..13077
                     /locus_tag="ACHA25_001867"
     CDS             12316..13077
                     /locus_tag="ACHA25_001867"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8763157.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13192..13632)
                     /gene="fabZ"
                     /locus_tag="ACHA25_001868"
     CDS             complement(13192..13632)
                     /gene="fabZ"
                     /locus_tag="ACHA25_001868"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8763158.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            13852..14607
                     /gene="fabI"
                     /locus_tag="ACHA25_001869"
     CDS             13852..14607
                     /gene="fabI"
                     /locus_tag="ACHA25_001869"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8763159.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(14717..15769)
                     /locus_tag="ACHA25_001870"
     CDS             complement(14717..15769)
                     /locus_tag="ACHA25_001870"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8763160.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            15895..16815
                     /locus_tag="ACHA25_001871"
     CDS             15895..16815
                     /locus_tag="ACHA25_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8763161.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         4897 a         3633 c         2948 g         5365 t
ORIGIN      
        1 ttaaaatcgc ttctttaaga ataacatcaa gaatagcttc ttttttctgc ttaatattcc
       61 attaatgtaa tgatgtcata tcctttgatt ttgtcgcgtc catggagatc catcaattcg
      121 atcaaaaatg cgcagcctac gactacaccg cctaattgtt cgatcagttc gatcgtcgct
      181 ttgattgttc ctccagtagc tagcaaatcg tcacaaatca gcacacgttg acctggttta
      241 atggcatcct tatgcaatgt caatgtatct gttccatatt ctaatccata agtcacttcg
      301 atggtttctc gaggtaactt cccttttttg cggactgggg caaaaccgac tcctaactca
      361 taagcaacag gacatcctac gatgaatcca cgggcttctg gcccaactac catatcaatt
      421 cgtttttctt ttgcataatt tacgatttgt ttagtagctt cccgataagc gtcaccatct
      481 gccatcagtg gggaaatatc tcgaaacaca attccttttg acggatagtc gggaatactg
      541 gcaatataat cttttaaatc cactctttgt ttcctcctag gttgttaacc atttctttat
      601 ggtaggaata tcgctcaaaa gcaaaaattc ctcaactttg attttattga gccgtcgctg
      661 gtataatctg ctctcagtca aaggatgact ttctgggcta gcgacttttt gtaaaacacc
      721 gttctctatt gtaacaaatt tcaattcaaa aaacacctgt atcataaaaa ttaataattt
      781 ttcagggata cgcaaatatt gagctattgc ttttatttta tgacgtatat caattcgttc
      841 ttgagaatgg atcaatttaa acaattttgc gtactgttct cttgtgccta ccccattcaa
      901 ataggcctca tcaggtgaaa tccccatcag ataaacacga ttgaatgaat aagaccgaaa
      961 aacttctttc agatccgcta aatcatctgg acaatccata acaactagag agtttggttt
     1021 tcttttttca attattgcct ttacagaatc tagtccttcg aaaacaatcg cagcttcctg
     1081 gataatctca ggcacgaatt tcaacgtcga agaatcaaat gctaaataaa ggctaccttc
     1141 cgacgttcgg ctctgttcac ggaaacgttt tgcccgccaa tcaaatactt gcagaccacc
     1201 cacagaaaaa tcagatacca tcagctgcgg tttcttacgc ccgttccatt cattgatcga
     1261 aagctttcct accacatcta cctgattatt cagcagttct tcttcttgag taccaaaacc
     1321 aaaagcgacc gcatcaagct gactcgatgc atcggaaagg acaaatttca gatgctgttg
     1381 gtttgctccg attcttttga tattttctgc ttggacttgt cgaaataaaa actgaggcaa
     1441 aggattatcc gtaccaaatg gcgccaatag tttcagctca tcaattcgct ctactgttac
     1501 ttcatttggc agcaatactt catcaattct caactcaggc cctctcatca agtcaatttg
     1561 atgatcgact atatattggt tcatcttctc ctgtagtata gtgacatttt cacttggcat
     1621 cgttaaaccg actgctgcat gatgtcctcc gaagtaagta aataaatctc gcatctggtc
     1681 aagcatttca aaaagattca aggcttcaat actacgtcca gatcctttag ctgatccatc
     1741 ttcttttaaa gtaaggacaa gcgttggttt acccgtttca ttcattattt tcccagcaac
     1801 aatgcccaaa acgccttcat gccatcctcg gtttaccaat agatggatat gattctcctt
     1861 attgaccatc gctaatgctt cttgtgttat ctgctctacg attgcttttc tttcttcatt
     1921 gatctcattc aatttttttg cttgaacgga tgcttcctct tcatcaaacg tcgcaagtaa
     1981 cgagacggca ggacgtggat cgcccatccg accgattgca tttagtctgg gagcaatcga
     2041 aaaaccgatc gttgtttcgt ctgcgtccct catttttacg ccgctttctt caaatagcgc
     2101 ctgaagtcct atccgttctg aatgatgaat ggcatcgatt cctaatgaaa caaggattcg
     2161 attctcatca gtcaatgaaa ctaaatcggc gatcgttcct atcgctacta aatccaaaaa
     2221 ttctgcaggc ggttcttcca ataatgcaga agctactttg aatgctactc cgactcctgc
     2281 caaatctgga aatggatagt gccccgctgg atgacgaggg tgaacaatcg cataagcgtc
     2341 tggtaaattc tcaggtagtt cgtgatgatc tgtaatgatc acgtctacac cagcttgctg
     2401 agcgtatgct accgcatcat ttcctgctac cccgttatca acagtgatga tcaactggat
     2461 tccttcttcg atcttttctt ggtataccga ttggtttggt ccatacccat gctcaaatcg
     2521 atttggcagg aatacttcta cttctgcacc aagtaattcc agtgtttctt tcattaccgt
     2581 tgcactggtg atcccatctg cgtcataatc accgtaaacg aggatttttt ctccactgat
     2641 tacagcttct tggattcggc tgattgcttt atccatatcg tgtatcagat acggatcatg
     2701 taattcttgt tcctttggat gtaaaaatcg gttgatatcc ttttcctcat ggtaacctct
     2761 ttgccacagt aacttaccga caaatggaga taatttcagt tcacggatca tttggagaaa
     2821 ggattcagaa ggctctgtct cgcttaactt ccaatcaaaa tttgcttgtc tcactttctc
     2881 actcctaact tagttattat agcaaaggaa atcagcaaaa aaaagcagaa agaaagaata
     2941 aaaacagatc agatccgcta gacagatgtt cagcaggacc tgatcttttg tacgaccatt
     3001 tatgaaatat ccctattagt caaaataatt gaagctttgt ttgggcgatt gagttttttg
     3061 ttcttccggc gctttcaatt gactgatttg tgcggttttc gcttgtaact ccgcctgata
     3121 agcagcttcc aactccgcac gttcgtttct aaactcgcgt tgctgttctt cttttgcttc
     3181 ttcaattttc ttttgaagtt ctgtttgata ttctgacatt tcctgagtca gctgtttgat
     3241 ttgctttttt tgctgccata gtgttgccga agcagtcaaa aatacaatca gtgcaccaat
     3301 cagcgcagaa ccgatgatca ctaagatcaa tggcgcagaa aaggaagtga agccaaaact
     3361 tacaggaaca tttttattgt tcaatacagc aaaaagaaca ataatcaaaa ctaaaacaaa
     3421 tccaagaatc acattgcttt gctttttcaa ttccttcacc tactttttgt taaaaattcc
     3481 gctggctaaa taatcaccaa tcgttggaaa cagcgtataa aattttgatg caacttctaa
     3541 aataaacggt tgattgattt cccgttttgg atgtctcata ttgcggacga tcatttttgc
     3601 caacttggca ggttctaaaa cgatttgtcc gactttttcc aaatagcttc cgcttggatc
     3661 agccttatcg aagaaatttg tctcgattgg acctggattc actgtcgtaa cagctacgcc
     3721 aagtggtttc aattctaatc gcaaagcgtt ggagaagcca aggactgcaa atttcgtggc
     3781 agaataaacc gttgattttg cagttgccat ttttccagcc atagaggcga tattaatgat
     3841 atgaccttgt cttctttctg ccatatcgat cgcaaatttt tgtgtcaaaa ccatcattcc
     3901 taagatgttg acttcaaaca tatcgtatgc ttttccaagg tcgaatgtaa ggaagtcttc
     3961 gaaaatccca aaacccgcat tgtttaccag aatatcgacg cgcccgactt cttccatcac
     4021 cttttcatat aattcgtcca cgctttcagg attgctgacg tctaccttga aagcataagc
     4081 ctctttgccg ctcaagcgtc tgcactcgtc tctgacacct tctacaaatg ccattcttcg
     4141 tgcgcaactt ataacgattg ctccttgctt tgctgcttca taacagattt gtgcgcccag
     4201 accgcctgaa ctacctgtta ctaaaacaac tttatcttct aaattcatgc ctcttcctcc
     4261 tcgcgaaatg gaatctcaat gctatcaaaa tctttcataa tcgtcgtgtt ttcaaacact
     4321 tcttgtgctt ctttttcaag ctctaaagct gcttttccta aatatctcgc actgatatga
     4381 gtaagtagca gccgttttgc ctgcgcttct tttgcaactt cagcagcttg atgagtcgtg
     4441 gaatggaaat agttgtgcgc cattcttgct tcatctttat taaatgtgct ttcatgtacc
     4501 agaatatcgc tattttccgc aagcacaacg gaattatgtg tttttctcgt atcgcctaaa
     4561 atagtcacga ttcgtccttt tttatctgga cctacgaaat cttttccatt gatcgtccgc
     4621 ccatcttcta gttggatcgt ttcaccttgc ttcagtttgc cgtacagtgg tccagctgga
     4681 atacctaatt cttttagacg atccacctgc aattctcctt tatgatcatg ttcaaccaca
     4741 cgataaccaa aactgtcgat cccatgattt agtttttttg tataaacaga aaattgctga
     4801 tcagtaaaaa tcggggctgt ttcatttagc tcaatgaatt tcagcggata acttaaacgc
     4861 gtttgcgaaa tacctaaaga gacttttatg tattcctcta tcccttttgg cccgtaaatc
     4921 tctaatggcg tatccccacc ttgaaatgac cggctgctga tgagcccggg aagtccaaaa
     4981 atatgatcgc cgtgtaaatg tgtaatgaaa attttaccga tttttctcgg acgaatagtc
     5041 gttcgcaaaa tctgcatttg cgtgccttcg ccacagtcga acaaccaaac ttcattgcgc
     5101 tcgtctaaaa gtttcaatgc tatacttgtc acatttctat gttttgcagg aacacctgca
     5161 cctgttccta aaaattgtaa ttccataatc tacctgactt tctttaagtt cctctttaat
     5221 tttagggtaa aaagaaggga taatcaaccg taagtcgaga atgggttcaa attaaaaata
     5281 acgttctaaa gcgatagcgt tttctttgaa aattactata aaaacagcag cgaacccgtt
     5341 tgagatcctc tgctgttttt tattaatttg aagatccaga actttcattg tcgttggaag
     5401 tttgcggatg tttgtccttc caagtctttt gtgcagcttc tttcgcatga ttttcagctt
     5461 ctttcgcatc atgcacatct ttttcgacat cttcataatc caagcgagta atttctccgc
     5521 ctaattccat cattaccaat tgattcagtg ggcgattgtc ctcttcatca gcaatcaaga
     5581 tcagtcctgt agctccttca ccgattttat ttcctacaaa gtcgaacacg gtctgggcat
     5641 cttttatttc tttggcatct tgtgtcgcac cgatcattcc tccggcaaac catcctaata
     5701 aaatccctag cggaccacct aatacaccaa ccaacattcc gattaatcca cccgtagaag
     5761 atttgttatt accggtaaag tcgataaaat cttctatttt gaattgatgt gtcccattag
     5821 gatcatggct gacaactgcc atctgttctc ctttaatcga acgaacagca tgtaactttt
     5881 tgatttctga aaatgcctga tatgcctggc tttctacttg aaaatttaaa atgattactc
     5941 gtttgctcat taaaaacgcc cctttcatac cttcttgtta tttattttac ctttaattga
     6001 aaaataagaa aagaaaagca ctcttttttt ccaataaaat agggctgtga caaaagtcgt
     6061 atcacagctc ctttttccga ataaacggcg ttcttgaagc tgatcacttc tgtcacaacc
     6121 tcccttaaat ctgaatctat attttttggt atttgttttt atttgcttgc ctattcgaca
     6181 aattcaaatt caaactcacc gatacgtaca aggtcgccat ctttcgcacc gcgtgcccga
     6241 agagcttcat ccacacccat accacgtaat tgacgagcaa aacgcatcac tgtttcatca
     6301 tgatcgaaat tcgtcatttg gaataatttt tcgatcttct caccagataa gatccacgtt
     6361 gcatctgaat cacgatcgat ttggaactca ggaccttctg gattgaatcc atagtgtaca
     6421 gtttcttctt ccagttcttc ttcgtacaat gggaattctg gcgttacttc taacaaatct
     6481 gctgtagcat tcaataacgc gtccaatcct tgtcgagtca ctccagaaat tggaaatact
     6541 gggatgtcat cagcaaattc atcttctttt tccttgttca gctgttcttt gaatttcact
     6601 agattttccg gtgcatccgg catatccatt ttgtttgcca cgatgatttg cggacgctct
     6661 agcaagcgta agttataagt agataattct ttgttgatgg caagatagtc ttcataagga
     6721 tctcgtcctt ccatcccact catatcgatc acatgtaaaa ttactcgtgt tctttcgata
     6781 tgacgaagga attgtgtacc cagcccaaca ccttgcgaag ctccttcaat caagcctggc
     6841 aagtctgctg cggcaaaact tctgccatca cttgtagtaa ccattcctag attaggtacg
     6901 agcgtcgtga aatgataagc gccgattttt ggacgagcag atgaaataac ggatagtaaa
     6961 gtcgatttcc cgacagatgg aaaacctaca agtccaacgt ctgccaatac tttcaattct
     7021 agttcgattt ttcgctcttg tcctggttca ccgttttcag caatttccgg tgcaggattc
     7081 cgaggagagg caaaacggat attccctcgt ccaccgcgtc caccttttgc cacaactagt
     7141 gtttgaccat tttcaattaa atctcctaaa agagcaccag tctccgcatc tcggacagtg
     7201 gttccttgtg gtactttaac ataggtatct tctgaaccac gaccgtgcat ccctttactc
     7261 atcccatttt cacctggctg cgctttgaaa tggcgattga accgaaaatc catcaatgta
     7321 cgtaaccctt catcgacgat caagatgaca tcgccgccac gaccaccgtc tccgccagcc
     7381 ggtccgccgt caggaacgta tttttcgcga cgaaatgcaa ccattccgtc tccgcccttg
     7441 ccagctttca catcgatcgt tacttgatct aaaaacatgg acataattaa gtcctcctat
     7501 tattcattta tttatttgac tgcctgaatt tccacaataa acaaaagtct tacttatttt
     7561 atcggtgaaa tctgattttg tctatcattt cagcaaaaag attgtttacc ttcttctttt
     7621 tttatcattt ggtaaggttt ccaacctttt ttctgctacc aagtataatt ttttgacaaa
     7681 atattctttg cgtagcagaa taacagtgta ttaattgtat taattgagga tgccgtttca
     7741 cagcacccct aataaaggag gaacttttga tatgacagta aaagtaggta ttaacggatt
     7801 tgggcgtatt ggccgtctgg ctttccgccg catcaaagaa gtctcagatg atattgaagt
     7861 agtagcaatc aacgacttaa caagtccaac tatgttagca caattattgc aatttgactc
     7921 tactcatggt acttatcctg gcacagttac tgcaacagaa gactcaatcg tagtggatgg
     7981 cgaagcaact cgtgtttatg cagaacccga cgcaagcaaa atcccttggg caaaagaaaa
     8041 cggtgtagat atcgtactag aatgtactgg tttttataca tctgaagaaa aagcaaaagc
     8101 gcatttagat gctggtgtca aacgagtagt tatttctgcc ccagctggtg ctatgaaaac
     8161 catcgtctat aacgtcaatg atgatacatt agatgaaaac gacaaaatca tctcagcagg
     8221 ttcatgtaca acaaactgtt tagcacctat ggcttatttc ttaaataatg aatttgggat
     8281 cgaagttggt acaatgacaa cagttcatgc ctacacttcc acacaaatgc tcttagatgg
     8341 acctgtaaaa ggcggtaatt tgcgtgctgc tcgttcagct gctgacaata ctatcccaca
     8401 ttctactggt gctgcgaaag caatcggctt agttatccct gagctacaag gaaaattaca
     8461 aggacacgca caacgtgttc cagtcgttga tggttcatta actgaattgg tttcagtatt
     8521 gaaaacaaaa gtaactgctg atcaagtaaa cgaagcaatc aaaaaacaca cagttgacaa
     8581 tccttcattc gggtatgatg accgtcaaat cgtttctagc gatgtcattg gaacaactca
     8641 aggttcgatc tttgacccaa cgcaaacaga agtaacaact gctggtgatt tccaacttgt
     8701 taaaactgtt gcatggtatg acaatgagta tggatttact tgccaaatga ttcgcttact
     8761 agaaaaattc gctaacttgt aatttgatag atcaatcgat aaaagaagcc tttgactgga
     8821 tccagtcaaa ggcttctttc ttttcatccc ctacttttaa actcgttcaa tgaacatatt
     8881 ggcttaaaaa ttgttggact acttgctgat atttttcagg atctgtcttg tatgcttccg
     8941 catgctcggc tccttttaca acatactttt cttttggtcc cttcgttgca cgatagacct
     9001 tatctaacat ttcaaatggt acaaatgtat cttcgtctcc atgtataaag agcattgggc
     9061 gctggttttt cttcaattgt tccactgcat ttgcttcacc gaaaaagtag ccagcacgga
     9121 cttttgtcac tagactcgtt acttgcataa gcggaaatgc tggaagacta aacatatcgt
     9181 ctagctgata agcaagctca tcatgaacgg aagaataacc gcaatcttca atgattgctt
     9241 tcacattatc tggtagtttt tctccgctag tcatcataac tgttgctgct cccatgctga
     9301 caccgtatag aacgatttct tgcgattttc cattgttttc tagcactttg tttatccact
     9361 gtacatagtc tttacgctct ggccatccaa atccaatgta atccccttcg ctttttccat
     9421 gtcctcttgc atcaggaaca aggacattgt aacccatatc atgaaacatc tttgcgtaat
     9481 tggtcatcgt ttcagcgttt cccatatagc catgagccac aagagctgtt ttttcacttt
     9541 tcgtttcagc aggaagataa atcgctgata atttcagacc gtcttttgaa gtgatggtcc
     9601 aatcgcttcg gttttctttt gaactaaacc atttatgtgc atccgtttct ttggatgtct
     9661 gtgttgtacc aggtgtatct tcgtctaaaa agtctttctc agaaggcaca acagcataat
     9721 tataaaaata attccctgca aacactaacg caacaatcag agctaaagcg acgcctaaaa
     9781 ggattttcat ccaaagtttc ataaatcatc catctcattt ctcttttttc atataacctt
     9841 gaattagtat aatcaaagaa tctctactat gcaatgttca tttttataac ccgcgaattg
     9901 taaaattaag ttagaaaaat aaaaaggcat ttatggtaca ctcaaattgt atttccgacg
     9961 aaagaaaaca aaggagtgta accataaatg acctacaaac atcttaccat agacgaactg
    10021 acaatgatag aatcatatta tcttcaacat aataagccgg ttgaaatcgc taaccgaatg
    10081 ggacgtgcta tacaaactat ttataatgta gtcaataagt tcaagcaagg caagactgct
    10141 cttgattatt ggcaccagta taaagaaaat aagaaaaaat gtggtagaaa agtcattcaa
    10201 ttacctgctc atgaagtaga ttacattaaa gagaaagtca ctcttggttg gacgcctgac
    10261 gtcattatcg ggcgaaaaga aaggtctgtt tcatgcggta tgagaacact ttatcgttta
    10321 ttttctaaag gaatatttga tattgacaca ctaccgatga aaggtaaaag aaaacccaat
    10381 ggccatcagg aaaaacgggg aaaacaacag tatcagcgct caatccatga tcgacctgat
    10441 aattatcctg atttcaattc tgagtttggt caccttgaag gtgatacgat cgttggcatt
    10501 catcataaaa gtgccgtcat tactttagtt gaaagattat ctaaagtcat tatcacgatt
    10561 aaacccaacg gccgtaaggc attagatatt gaaactgccc ttaatcaatg gttttctcgc
    10621 ttccctaaaa acttctttaa atctattacg tttgactgtg gaaaagaatt ttctaactgg
    10681 aaagccatta gtaaccaaca tgatattgat atatattttg cggaccctgg aacaccttct
    10741 caacgcccat taaacgagaa ttctaacggg attctgcgtc gtaatggact gccgaaatca
    10801 atggatttta gaaaagtgaa tcagacattt atttccagtg tcagcaatca acgtaatcat
    10861 attccaagaa aatcattgaa ttacagaaca ccaatcgaaa tatttttgag ctatgtacaa
    10921 gaagcatttt attctagctt aatttgacaa atcataacct ataaaaaaac aaaataaaaa
    10981 agcagaaata actggctatt tctagcggat tatttctgca ataactaatt atctattttt
    11041 tattttcaat taatttaaag cttgaccatg gtttcaaaaa gtcaaggagg aataattcgt
    11101 catcggagat tcttcctaca acattgacac gtccgtcgtt tttcatatcc ttcaaggcga
    11161 tttgtgtttc tcctttgtat tgaccgtacc cttcattgtc aatcagtaca tccccatgat
    11221 gcatatctcg cgtattatga ggcgggaaat ctttatcttt gtagtataca cgagtcatcg
    11281 ttgaacgaag gatatattct gagcgatccc ctcgataact atgaaggtta tcaaacaagc
    11341 agattcgttc gttttctgta ataccttctt ccgtatctac tttcaatgtt ggataatccg
    11401 cgttaaatgc ttctgccatt tctttcaatt cttcttcact tgcgtacgca tttccaatag
    11461 aaatatcatc aatcaatcct gtaaggatta aatgcttcac ttgtgtcgca atttcaagat
    11521 ctcggtggtc ttccagcgtg cacaagccat cttgcgtagg ccaagggcca aactcagcag
    11581 attgcgaatt gacgaatgcc atcgtattca aattatattt tctgaatttt tctgaacaaa
    11641 aaacaaagtg ttcgtatcct aaaccagagt atcgatgagg ataaaaatta tgagaaccta
    11701 ataaattatc cgtgtttggc gaataactca tgatgttgtc aacgtaattc gtgcccgaac
    11761 tcatattgat ctcgattttg ataccaaaag gattgcgcgt catttttgct tcttctgctc
    11821 ctgtgaatcc gatatctaag cggaccccgt aggcacccat cttatggaaa aaagataagt
    11881 catcgtaaga aatctccaac tgttcaaata acgctggatt gatatcaacc attacttcca
    11941 tgcctaggct attcgcgtaa tcaactactt ctttaaattc tgaaagaact ttttctttat
    12001 catcattgat ctgcagcaaa ctggtaaaaa cccgtttata cccatactta tgagctaaat
    12061 ccaagtacgc tttgtccttt tcaaatgttg accgttctgg ataaatcgag attcctaatt
    12121 ttcccatatt accatttcct cctgcatctt ttcgtttttg tatccactta cattatagca
    12181 aacacaaagt aaatggtcta caccttttct ttttctttta aaataaatga taaattccaa
    12241 taatttaagt aggtattttc cctctatttt gttatcatta aagataacaa aatagtaaat
    12301 tggaaaggaa cagttatgaa gaaaaataaa gttattttaa gtattagttt agcagcagca
    12361 ttgctgtttg ctgctggttg ttctgcaaac aacacgactt cttctagttc gtctacagaa
    12421 agttcaaaaa gtacatctac tgcggaaagc acgactgcca gtagcgtcga tttgaacgca
    12481 ttggaattac cacaattgac gaaagatgtc gccgaaaatg aagatctggt cgaaatggtg
    12541 acgaataaag gcacaatcaa aatcaaatta tttcccgaac acgcaccaaa agcggttgaa
    12601 aactttatga cacatgcaaa agacggttac tacaacggac tgactttcca tcgcgtcatc
    12661 aaagatttca tgatccaagg tggcgatcct aaaggcgatg gcactggagg ggaaagcatt
    12721 tggggagaag gatttgaaac agagatcaac aaccaccttt ataatataag aggtgcgtta
    12781 tctatggctc gctcacaaga tcctaacagt aacggcagcc agttctttat cgttcaaaat
    12841 acagatgata tgcatgatgg tcttttaaaa gacgactatc ctcaagcaat catcgacgct
    12901 tataaaaacg gcggttatcc ttcactagat ggccattata ctgtcttcgg ccaagtgatt
    12961 gaaggaatgg atgtcgtgga tgctattgca aatatcgaaa cagatagttc agacaaacca
    13021 aaagaagaag taaaaatcga taaaatcaat atactgcaag aagcaaaaca aaaataaaaa
    13081 aataacgtct gaatcctgca tagatgacaa cgtatgctag gattcaggcg tcttcttttt
    13141 acataaaaag caagtaagct tccttttgtt ttgctatatc aggttttata gtcaagcatt
    13201 ttggttgacg atgaacgtga actcgcactc acagactttt ttatcatcta catatgctgc
    13261 agataacgct gtaccgactg atcccttcat tttggtgatt tcaaaatgac atttcagcac
    13321 atctcctgga acaacttttt gacggaattt ggccttattg atcccaccga tataagctct
    13381 ttttcctaga aactcttctg attttaggat aagtatcgaa cctgcttgtg ccagtgtttc
    13441 tagaatcaac gcacccggca tcactggatt gtcaggaaag tgaccttgga aaaaatcttc
    13501 attgattgtt acattttttg tcgcaacgat cttttttcct ggttctaaag tatctacata
    13561 atcaatataa caaataggat aacgattagg aatcaattcc attacttcag tagcagataa
    13621 tacgtaagac ataattaaac tccttctttc aggcaattgc cattttcatt actttgacta
    13681 tcaaagtatc atattcctaa atatttagca atcttttttt aatttaagaa aaaatagttt
    13741 gacaatcaaa acattttgac taataattaa gaaatcaaga agttatttta gctattattc
    13801 tctatttttt tacgactatc aaatttttat cagaaacaaa ggagagtcat tatgtcattt
    13861 ctagaaggaa aaaaaatcgt cattatgggc gtcgctaaca aaaaaagcat tgcttggggt
    13921 tgtgctgaag caatgttgaa acaaggggca gaaattatct atacttacca aaatgaacga
    13981 atgaaaaaat cattagtcaa attggtcggc gaagaagcat tcactgtaga atgtgatgtt
    14041 tctagcgacg aaagcatcga aaaagcaatg aatgccatcg gtgaatatgc tggggagatc
    14101 cacggccttg tccatgcagt tgcttacgcc aacaaagaag aattgtccgg aaatgtgtct
    14161 gatatttcca gagacggcta tcaattagct caagatatca gcagctactc tttaattgct
    14221 gtatcaaaat acgcacaacc gtttcttgca ccaaacagcg ggatcgttag catgacttat
    14281 ctaggttcag aacgcgcaat cccgaattat aacatgatgg gtattgcgaa agcttctctt
    14341 gaagctgcta tccgttattt ggcagctgag ttctcgccta aaggaattcg ggtcaatgga
    14401 atctcagctg gggcaatcaa aacattagct gtcacaggtg ttaaagacta tcaaaaactg
    14461 atccagttat ctgaatctcg tacgcctgat cagcaaggtg tgacgatcga agaagtcggt
    14521 aatgcttgtg ctttcttaat cagcccactt tccagcggaa tcatcggtga tattatctac
    14581 gttgacaaag gtgtccatct ttcataaact tttgttgcat tctttaaagt aaaacatata
    14641 aaaatcagga aaaaactttt taaagtctct tcctgatttt ttctttttac gtttttttca
    14701 actcattact ttgaaattat tccacgactt caatccagcc ttctggtgct tctatgtctc
    14761 caaattgaat acctgtcaat tcatcataca accgttttgt tactggtcct acttccgttt
    14821 cactataaaa tacatgaaga ttgttattgt gataaatccc accgattgga gaaataacag
    14881 ccgctgtacc acatgcacca gcttctgcaa attgatctag atcatcaatg aaaatatctc
    14941 cttcaattgc ttgcagtcct agtcgttctt cagctaactg caacaaggaa tatttcgtga
    15001 tacttggtaa gatagagggc gattgcggag tgatgaattt tccgtctttt gtaattccaa
    15061 agaaatttgc tgcacctact tcttcgattt tcgtatgtgt ggctggatca agataaacac
    15121 aatctgagta atccagttct tttgcttctg ttcctggaag taaactcgct gcgtaatttc
    15181 cgccgacttt tgctgcacca gtccccttag gagcagcacg atcgtaatca gatacaataa
    15241 aattcgttgg ttttagtcca cctttgaaat acgctcctac tggcgtacag aagacgacaa
    15301 ataaatattc tggagctgga tggacaccaa tgttttcacc gaccccaatc agtaaagggc
    15361 gcaagtataa tgtagcgcct gttccatagg gcggcacaaa atcttcattt gcttttacca
    15421 cttgcttgac agcatcaacg aacatctttt cagggaccat tggcatcagc aatcgtctag
    15481 cactttgatt catccgtcga ctattttgtt caggtcgaaa gagattgacc ttgccgtctt
    15541 ttcgacgata agcttttaga ccttcaaaac attgctgacc ataatgaagc gctggagagc
    15601 cttcatgaat cgtcaggtaa ttatcttctg tcaattctcc ttcttcccat tgtccgtctt
    15661 tccatttggc aataaaacgc caaggagttt tgatataaga aaaacctaga ttgttccaat
    15721 cgatttgcat atacttacca ccttcgttat tttctccaca ttttatcatt atccatatta
    15781 tttgtcattt tattttcagc ttttttcctt cattatagtt atttttgcta tttccttttt
    15841 tgaaaacttc ttggtacaat acagaaagaa tcaaatttta gaaaagagga ttttatgttt
    15901 tcattattag caactgccgg tacgacagac tcttctggcg agacattgac agatcaagct
    15961 gtcagcaaag taaatgcgtt tcagcggttt tgggacggga ttgattggga cgccattttg
    16021 gctgccttta tccaaaaaag tttatatctg atttttttga tcgtcctttt ttggattctt
    16081 caccgtgtgg gaaaatatct gattgataag tcttttaatc agtattctaa aaaagccatt
    16141 ctaaacgaga gccgcttaaa gactttacat agtcttttaa atactatttt tcattatacc
    16201 cttggtttct tttttatcta tgcaatttta tctgctatcg gtgtgccgat cggatcactt
    16261 ttggctggtg ccggaattgc aggtgtggca atcggtcttg gtgcccaagg gttcatgagt
    16321 gatgttatta ccggattttt tattatcatg gaacaacaga tggatgttgg ggactacatc
    16381 aagctagcaa acttaacgat tgaaggaacc gtcgtatctg ttggtatccg tacccttcag
    16441 ctgaaagcaa cggatggtac tgtccatttc atcccgaatc gaaacatcac cacgatcagc
    16501 aatctctctc gtgcaaatat gcaagtactt ttagatattc ggattgttcc tgaagaagga
    16561 tatgacaaaa tctatgagat cattgatcga gtaaatcgta cattagctga aaaataccaa
    16621 gaagagctcc aaactgaacc ttctatcttt ggattagtgg atcttggaaa cagcaatttt
    16681 gcgattcgta cggtctgcta cgcattgaac ggaaaacaat atgccttaaa agaagagttc
    16741 ttgagctcct atgtcaaaga attgacagct tctggtttca cgattccgaa cagtccaatt
    16801 gctgtcggaa aataatttgg acaaacgatg caacgaatct cac
//
LOCUS       DAYYFD010000059        16816 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000059 DAYYFD010000000
VERSION     DAYYFD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.059"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..791
                     /locus_tag="ACHA25_001872"
     CDS             39..791
                     /locus_tag="ACHA25_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8763162.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            788..1702
                     /gene="pfkB"
                     /locus_tag="ACHA25_001873"
     CDS             788..1702
                     /gene="pfkB"
                     /locus_tag="ACHA25_001873"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8763163.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1720..3651
                     /locus_tag="ACHA25_001874"
     CDS             1720..3651
                     /locus_tag="ACHA25_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8763164.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3762..5321)
                     /locus_tag="ACHA25_001875"
     CDS             complement(3762..5321)
                     /locus_tag="ACHA25_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8763165.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5476..7710
                     /gene="pcrA"
                     /locus_tag="ACHA25_001876"
     CDS             5476..7710
                     /gene="pcrA"
                     /locus_tag="ACHA25_001876"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8763166.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7727..9763
                     /gene="ligA"
                     /locus_tag="ACHA25_001877"
     CDS             7727..9763
                     /gene="ligA"
                     /locus_tag="ACHA25_001877"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8763167.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9869..10174
                     /gene="gatC"
                     /locus_tag="ACHA25_001878"
     CDS             9869..10174
                     /gene="gatC"
                     /locus_tag="ACHA25_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8763168.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10174..11646
                     /gene="gatA"
                     /locus_tag="ACHA25_001879"
     CDS             10174..11646
                     /gene="gatA"
                     /locus_tag="ACHA25_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8763169.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11643..13073
                     /gene="gatB"
                     /locus_tag="ACHA25_001880"
     CDS             11643..13073
                     /gene="gatB"
                     /locus_tag="ACHA25_001880"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8763170.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13083..14138
                     /locus_tag="ACHA25_001881"
     CDS             13083..14138
                     /locus_tag="ACHA25_001881"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8763171.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14249..15625
                     /gene="rlmD"
                     /locus_tag="ACHA25_001882"
     CDS             14249..15625
                     /gene="rlmD"
                     /locus_tag="ACHA25_001882"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8763172.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            15834..16670
                     /locus_tag="ACHA25_001883"
     CDS             15834..16670
                     /locus_tag="ACHA25_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8763173.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
BASE COUNT         5680 a         3036 c         3694 g         4406 t
ORIGIN      
        1 aaaaatatgc tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac
       61 aaaaaatcct agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc
      121 tttttaatgc ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt
      181 ttctagaaag agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata
      241 tgagtgaaaa atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg
      301 aaaccattaa tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga
      361 ttccttttct gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa
      421 atttagttga tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta
      481 atgctgtttt aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat
      541 ttatgggaat gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg
      601 cagctgttaa acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata
      661 ccaaattcaa ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa
      721 ccgagcgttg tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg
      781 caatacaatg atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga
      841 tgggttaaaa ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa
      901 agggatcaat gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt
      961 tttaggaggg ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc
     1021 tttgttcaca accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga
     1081 aactgaaatt aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca
     1141 agcattagga acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag
     1201 cctgcgaaaa ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt
     1261 cgtgattgat acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat
     1321 caaaccaaac aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga
     1381 aatcattccg tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat
     1441 ggctggagac ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt
     1501 tcgcccattg aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc
     1561 ttacagccgt acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc
     1621 aacagctttt tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt
     1681 tgaaatcaca aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg
     1741 gtcaaagaag cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa
     1801 atggtccaaa aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga
     1861 attctagccc gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac
     1921 gcgaaaaaca gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta
     1981 gattatgaag ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa
     2041 ggcgcaaacg acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca
     2101 gatttcgtag gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa
     2161 acagctgaag agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa
     2221 gcttctgctc atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc
     2281 gctcacactt acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag
     2341 atcaaagtcg aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc
     2401 gcccgtgcag aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac
     2461 ggaaaagaac tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc
     2521 aatctagccc ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca
     2581 gaagaaggaa atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc
     2641 gtatcacaca tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg
     2701 atcgaccaat ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa
     2761 gctgctagct ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta
     2821 gcaggattta ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct
     2881 ggtgcattag ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta
     2941 gctggttatg tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat
     3001 ggaattaaaa cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg
     3061 ttgctggtca atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt
     3121 atgagtggtt cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat
     3181 ttaggcggtc caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact
     3241 gttgcaactg gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca
     3301 ttggcaattt tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat
     3361 gctggtttga cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt
     3421 gcagcagctg acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc
     3481 ggattagtcg gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta
     3541 ttcttactga gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct
     3601 ggtgtgattt atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa
     3661 agaagaggct ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag
     3721 tgtagatgaa tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa
     3781 tttgtgatag aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca
     3841 gtcaagataa ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc
     3901 tgatttcctc tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc
     3961 attaatccgt ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct
     4021 tttagtgagg cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga
     4081 ttaccgccga gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc
     4141 agatgattaa gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt
     4201 ccaatcgctg tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga
     4261 tccatcccga aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc
     4321 aatatcggaa ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg
     4381 aatcgaagaa caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct
     4441 aaaacgattt gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc
     4501 agcaagaaag gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt
     4561 ggcaaagcca gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag
     4621 atcactaagg aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca
     4681 ggtctcaagc cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat
     4741 gtcagccaaa caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct
     4801 gcgataggag cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc
     4861 ttttcttctg aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca
     4921 agctgtatag aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg
     4981 ccaatgaact ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt
     5041 ccctctactt gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc
     5101 acgaaaaatg ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc
     5161 cgactcattt catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac
     5221 aggctaacga cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc
     5281 tgagtcttat ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt
     5341 ttaatttagc gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat
     5401 tttgaactag ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa
     5461 gaaaggatgt tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag
     5521 aaagaagctg ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga
     5581 aagacgagag tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct
     5641 tggaacatcc tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc
     5701 aatgcgattc tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt
     5761 gtgcgtattt taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc
     5821 gattcttctg aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg
     5881 aaaaaatatg atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag
     5941 acccctcagg attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt
     6001 tatgctgctt accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc
     6061 atgaatacga ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa
     6121 ttccattata ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc
     6181 aatttattgg caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt
     6241 tacggctggc gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat
     6301 gctgcagtaa ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca
     6361 aaccaagtca ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa
     6421 gaaggaaaca agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc
     6481 gtagatcgga tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc
     6541 ttgtaccgga cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt
     6601 ccttataaaa tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta
     6661 gcgtatttga atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat
     6721 tcgcccaaac gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt
     6781 catgaatggc cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa
     6841 gcaggacagc aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca
     6901 tacttgacag tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac
     6961 ttaaaaatcc aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta
     7021 acggtcacac aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca
     7081 ccagaagaaa aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat
     7141 ttagaagaag atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa
     7201 ttcccagttg tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg
     7261 atggaagaaa gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca
     7321 gaagaagcgt tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat
     7381 aaccgacctt ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg
     7441 cgtcctacgc ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag
     7501 ccagagacag cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa
     7561 cctggagata aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg
     7621 acgtcgaagg atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg
     7681 gctgcctttg cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat
     7741 gacgattgac gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc
     7801 tcgcgaatac tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata
     7861 cgcagaatta gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac
     7921 tcaacgagta ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct
     7981 ttacagtttg aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca
     8041 aaaagcagtg ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt
     8101 ttctttgcgt tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt
     8161 aggagaaaac atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga
     8221 accaatgaac atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt
     8281 gaaccaagac agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc
     8341 tggttctttg cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt
     8401 atacacagta gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga
     8461 gctagaaaaa attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga
     8521 agtgtggtca tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga
     8581 tggaatcgtc atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa
     8641 agcaccgaga tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga
     8701 aaacatcgaa tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc
     8761 agtccgagta gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa
     8821 gttgaaggat atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc
     8881 agaagtttcg caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc
     8941 tacacattgt ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg
     9001 gtgcatcaat cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag
     9061 aaatgcgatg aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa
     9121 actagtagca gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga
     9181 caaaataaaa gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa
     9241 ttcggtcgag cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa
     9301 aattttagca gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat
     9361 tattgcgctt gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa
     9421 cgaagaagtc catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa
     9481 agggcttcga aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt
     9541 ccttactggt aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct
     9601 tggcgggaaa gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga
     9661 tgctggcagc aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca
     9721 aatggcagat gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag
     9781 acaaatttca ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt
     9841 tttaactcgt ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt
     9901 tgccaaatta gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt
     9961 aggtaaaatc atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt
    10021 tacgtcgaat gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg
    10081 gaaccgcgat gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc
    10141 cgcaatcatc gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta
    10201 gaagaactgc atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca
    10261 acatttgaac gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa
    10321 gaaaaagcat tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc
    10381 cttgctggta ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc
    10441 gctgcatcta agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa
    10501 atctatcaag cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt
    10561 ggaagtacag aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta
    10621 cctggtggct cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct
    10681 ttaggtagtg atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg
    10741 atgaaaccta cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt
    10801 gaccaaatcg gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc
    10861 agcggttatg acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag
    10921 ggactgacag gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa
    10981 ggtgtggccc ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta
    11041 ggtgctgttg tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat
    11101 atcattgcct catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt
    11161 tatcgctcag aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga
    11221 ttcggtgaag aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat
    11281 tatgacgctt acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa
    11341 aaagtctttg cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga
    11401 atcggtgaga acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct
    11461 gtcaacttgg ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca
    11521 gttggattgc agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat
    11581 gcgtttgaac aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac
    11641 gaatgaattt tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa
    11701 aaattttctc tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga
    11761 tcgactgggg ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga
    11821 tgaaagcagc tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa
    11881 attactttta cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg
    11941 gtcatgacgg atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac
    12001 gtgtccattt agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg
    12061 ttgatttgaa ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt
    12121 caccagaaga agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag
    12181 tcagcgatgt gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc
    12241 cttatggaca agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt
    12301 tcgtgaaaaa aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag
    12361 gagaaatcca acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc
    12421 gtgtcaaaga agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg
    12481 tgatcgatga cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc
    12541 gtcgcgaacg ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac
    12601 ttacaaaaga aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc
    12661 aagcatctaa ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat
    12721 tagctgagac aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg
    12781 gaacgatcag ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag
    12841 aagcccaaga agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac
    12901 tgccaatcat taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg
    12961 gaaaagatcg tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag
    13021 caaatcctgg tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga
    13081 aaatgaaaaa agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa
    13141 atttggcgga tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa
    13201 ctacaccaga accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg
    13261 atctagtagt ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc
    13321 cgcttgaaca ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc
    13381 gagcattaaa aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc
    13441 aaaccgtaaa aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag
    13501 gtggctattt gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt
    13561 atcttgctta tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc
    13621 gcttgaagta tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga
    13681 cgaactctgt tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt
    13741 tttctttaat cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca
    13801 tgctgaatgg cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt
    13861 tgttcgttga aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat
    13921 acggcgggga agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca
    13981 ataccgatca gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg
    14041 tcagcaaaga attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg
    14101 gaaaaatagc agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta
    14161 gtaaggtcag tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga
    14221 ttgaaaaata ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga
    14281 acgatataca gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga
    14341 tggttatcca ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt
    14401 aaaagcaggc aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga
    14461 tcgtcagcct gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt
    14521 gaagtatgaa caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat
    14581 tgctaagatg ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta
    14641 ccgtaataaa gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta
    14701 caaaaagaat agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat
    14761 cgatcaagca atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa
    14821 tgaagaaaaa aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca
    14881 tgaaatgatg gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc
    14941 tgaagctatt catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga
    15001 aaaaacaaat gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga
    15061 agatcagcta ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac
    15121 tcaacaagca gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga
    15181 cattgtgatc gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt
    15241 ggataaggtg tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc
    15301 tttaaataat aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa
    15361 aaaatggcaa gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg
    15421 attagatcat cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat
    15481 ctcatgtaat ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc
    15541 cgtgaagaaa gttcagcctg tagatctttt cccgcaaacc catcacatcg agtgtgtagg
    15601 actgcttaca cgtgtggaac gttgatatga caaggattag agcgattcgg gatggtttcc
    15661 ggattgctct tttttcgtgt tgaaatgacg atttttgggt gagcgtagag ttgatggaaa
    15721 tgctatggag ctttgagttg ataggagtta caccgtagac ttgataggtt agatgatttt
    15781 gataacggtt acgtgatata atatattatc gagttttcaa gggagacaaa cttatgaagc
    15841 aaatttttat attttcagat acacagaaag ataatatttc ttattttgaa cgcgagccag
    15901 caatcttggt ttcaatgagt cgtagtgatt tgccgtattt gaaggaacag gatttttcaa
    15961 attatccagc tgtttatgtt ttgattggtg gtaataagcg ttacgtgggt caggcagcag
    16021 ggcaatcaat ttcacttcgt ttatctcaac actttctcaa ggaatataaa gcttgggttg
    16081 aatcagtttt attctttaca agagcagatg ggaaaatgtc aaaagcagat acagattatc
    16141 ttgaaaaacg acttatccaa gatttccaag aaaaatctga ctatgaaatg atgaattcaa
    16201 ctgctggtaa tacttcgtat attgataaac ttcaaaaagc aaaatcggat cagctatatg
    16261 acacagtgtt tgaaattata gatgaaattg caaatatcga tttgtttggt acttccgaag
    16321 acagtgaaac ccaaatttat gaatttagtt catctggaca atatgaaatt gagtatgatg
    16381 ggaaaaagat atcttcgaaa tctgctagag gtttattggt tgattttgtt tctcagctat
    16441 ttgcggatgg aaactacaac aatcaactgc aagaattaat tgtcgatgag acgccgtcgt
    16501 tcgctataat tttaggacgt aaaccatcca tgtataacgg aagaccaaat tcagccaggg
    16561 tagctgatgg agtatgggtt tatgcaaatt tcaacaaaaa gaatgtcaga atcaaaattg
    16621 ataaactagc tcgtcaattg gggattccgg ttaaaataaa gtggaattaa tatttgttat
    16681 ctcgccttta gtaaatctat gctgaaggct tttattttag aaaaatagtt tgcgttcgcg
    16741 aaaatatttc atgtttcgta taataatgat agctcataga tgatataatt gaagatacca
    16801 gtagaataga ggtgta
//
LOCUS       DAYYFD010000060        16773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000060 DAYYFD010000000
VERSION     DAYYFD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.060"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..726)
                     /gene="pyrE"
                     /locus_tag="ACHA25_001884"
     CDS             complement(97..726)
                     /gene="pyrE"
                     /locus_tag="ACHA25_001884"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8763174.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(733..1449)
                     /gene="pyrF"
                     /locus_tag="ACHA25_001885"
     CDS             complement(733..1449)
                     /gene="pyrF"
                     /locus_tag="ACHA25_001885"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8763175.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1446..2378)
                     /locus_tag="ACHA25_001886"
     CDS             complement(1446..2378)
                     /locus_tag="ACHA25_001886"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8763176.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2375..3151)
                     /locus_tag="ACHA25_001887"
     CDS             complement(2375..3151)
                     /locus_tag="ACHA25_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8763177.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3205..6390)
                     /gene="carB"
                     /locus_tag="ACHA25_001888"
     CDS             complement(3205..6390)
                     /gene="carB"
                     /locus_tag="ACHA25_001888"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8763178.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6395..7471)
                     /locus_tag="ACHA25_001889"
     CDS             complement(6395..7471)
                     /locus_tag="ACHA25_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8763179.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7476..8756)
                     /locus_tag="ACHA25_001890"
     CDS             complement(7476..8756)
                     /locus_tag="ACHA25_001890"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8763180.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8775..9701)
                     /locus_tag="ACHA25_001891"
     CDS             complement(8775..9701)
                     /locus_tag="ACHA25_001891"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8763181.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9714..10997)
                     /locus_tag="ACHA25_001892"
     CDS             complement(9714..10997)
                     /locus_tag="ACHA25_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8763182.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11011..11550)
                     /gene="pyrR"
                     /locus_tag="ACHA25_001893"
     CDS             complement(11011..11550)
                     /gene="pyrR"
                     /locus_tag="ACHA25_001893"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8763183.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11785..12696)
                     /locus_tag="ACHA25_001894"
     CDS             complement(11785..12696)
                     /locus_tag="ACHA25_001894"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8763184.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12693..13157)
                     /gene="lspA"
                     /locus_tag="ACHA25_001895"
     CDS             complement(12693..13157)
                     /gene="lspA"
                     /locus_tag="ACHA25_001895"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8763185.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13162..13860)
                     /locus_tag="ACHA25_001896"
     CDS             complement(13162..13860)
                     /locus_tag="ACHA25_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8763186.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13847..14233)
                     /locus_tag="ACHA25_001897"
     CDS             complement(13847..14233)
                     /locus_tag="ACHA25_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763187.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14380..16050)
                     /locus_tag="ACHA25_001898"
     CDS             complement(14380..16050)
                     /locus_tag="ACHA25_001898"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8763188.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16233..16466
                     /locus_tag="ACHA25_001899"
     CDS             16233..16466
                     /locus_tag="ACHA25_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8763189.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16565..>16773)
                     /locus_tag="ACHA25_001900"
     CDS             complement(16565..>16773)
                     /locus_tag="ACHA25_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8763190.1"
                     /translation="WKDREISVGQIRSLTPTNTGVQMMLQDEKAPSVFMMRKKDQVVL
                     FDHFEKNGPKSESIPYPYTKKRKS"
BASE COUNT         4547 a         3821 c         3001 g         5404 t
ORIGIN      
        1 aaaagatgct tctcgcaatc agtttttgga tttttgctat tccaattgat gattctggag
       61 acacaaatcg gatttctcta tcttcatcaa gcgcgcttaa atctgccaat tttcaggatc
      121 ttttttccat tcttttagca gagttaattc ttgctcatcg atataacgat cttctaaagc
      181 tgcttcaatc aatacagaat aattggtcaa cgtcataaga gggatttcgg ctttttcgaa
      241 atttgctttt ccttttggca actcatacgt aaagatagcc gcaacaccaa ggatatccgc
      301 accttctctt tttgctgctt cagctgcttc caagacactt ccgcctgtag aaatcaaatc
      361 ctcgatcaca accatcttct gaccttcaaa tatacgtcct tcgatttggt tacctttccc
      421 atgatctttc gccttacttc gaatatagac cattggcaaa tccagtattt ccgctaccca
      481 tgcggcatga gggattcctg ctgttgcagt tccagcaatc acttgtactt cagggtaagc
      541 ttctttgatt ttgcttgcta atccttttgc gatctctttt cggacttttg gatagctcat
      601 cgtgattcgg ttatcacagt aaattgggct tttgattcca cttgcccatg taaaaggatt
      661 acttggattt aaaaatactg cttcaatttc taataagtct tttgcgatac tttgttcaac
      721 attcatttta ggttagctcc attctgtttg aatcgtttga tatgctttat aaggattagt
      781 tgactgagtg attggtcgac ctataacgat aaaagttgag ccgatctttt ttgcctgaga
      841 aggtgtgact actcttgtct gatctcctgc gactgttcct tctggtctta tacccggagt
      901 taagcaaaca aattcgtctc ctgccatttc tttgatcgct tctacttccc aagcagaaga
      961 aaccacacca tctaatcctg cttttttggc acagctagca taatggatca cacttttttc
     1021 taaagaaact tcgattagtt gatcggcatg catttcttct tcacttgtag aagtaagctg
     1081 agtgatagct agaagtagcg gacgttttcc accttctgga gtcccctctt ctaatcctcg
     1141 cattgccgcc tccatcatac gaataccgcc agcggcatga acacaggtga gatcaacacc
     1201 aagctttgcc aatccccgca ttgctttttc gaccgtattc ggaatatcgt gaagtttcag
     1261 atctaaaaat acattatgac cagcctcttt tagataacgt acgatttcag gtccttcttg
     1321 gtagaacaat tccattccga ttttcacaaa caatttttct tctttcggga aaaatcgaag
     1381 aaagtcttct acctcttgcc tagttgaaaa atctaatgca ataattggtc gttggtctag
     1441 ttgattcatc gttcacgttc ctctctcact tctttgatta atctttccag cgattcgatg
     1501 cctagttcgc tcattcgcac agggagctct ttaattagtt taggacagat atacggatcg
     1561 gtgaaattcg cggtccctat agctacagca cttgctccag ccataaacat ctcaagaaca
     1621 tcatccactg tctgcacgcc gcccatccca atgattggaa ggctggatat acttgctact
     1681 tggtgaatca aacgaatagc gacaggcttg attgctggtc cggagagtcc acctgtttga
     1741 ttggctaaaa tcggttttct tgtcttaagg tcaatccgca taccaagaag agtattgatc
     1801 atcgtaaacc catctgcccc tccagcttcg attgcctgag cgattggaac gatgtctgtt
     1861 acatttgggg acaatttgac atagacagga acagaagaaa ctttctttac tgcttgtgtt
     1921 aattgaaaag caacatcaga atctgttcca aaagcaattc cgccatgttt gacatttggg
     1981 caagaaatgt tcagctcgat tgctttcaca tttggtgctt cgccaatttt gctacaaact
     2041 tctacatagt cttcttcaca agcaccagca acatttgcga tgatgggtaa ctcattgtac
     2101 tcctctaatt ctggaagaag tttatgcata acaacgtcta gaccaggatt ttgcaaacca
     2161 attgcattca acatgccaga tggtgtttcc gccactcttg gcgtgggatt gccaaagcgg
     2221 gcatttggag tcgttgcctt gaccatgatc gatccaagct gattcaaatc atagtatttt
     2281 ccgtattctt tgccaaaacc gaaacagccg cttgccggca tgataggatt ttttaattct
     2341 aggccaggaa gtttgaccgc taaaggattc tcattcatat aattacctct cctattttaa
     2401 aaatcggtcc ttcgtcacaa actttcacac tttttgttcc tgtttcatct tccggcgtat
     2461 gacaaacgca agcatagcaa gcccctatcc cacatgccat gcgggattct aatgataact
     2521 ggacattttg atgcgaagaa aacagctgtt ccactgtctt caataacccg tttgaaccac
     2581 atgcaaaaac agctgtaggt tccgtcttcg ttcctaacaa taaattaccg acatttccgt
     2641 gcataccgta tgttccatcg tcagtagcga ttcttgtttc tcctagacga ttaaattctt
     2701 tttcataata aacgacttca tgtgtggcaa aacctaaaaa atgcgttggc ttaattcctt
     2761 tttcgactaa ctgttttgat aactcatata gaggagggac accgattcct ccaccgatta
     2821 taaatacttc gtctcctgta tgaagattgg acaaatcaaa accattgcct aaaggaccca
     2881 tgacatctaa atggtctccg gcagaaagag tagagaagac tctagtcccc tcaccttcca
     2941 cccgataaat gatacggcaa gtatttgttt cgtggtcgat atgattcaag ctaatcggtc
     3001 ggcgtaatag caagtctgca cgcggcacga gaatatgcaa gaactgtcct ggttgctgca
     3061 tttcttttac tagatcacct ttcaatgtca tttcaaaaat ctttggtgcc agctgttttt
     3121 gctgaacgat ttccatcatt tcttgtttca tctgtcccct cctgcaacgg tttttcgtct
     3181 ttttgtctta acatgtgctc gccgttaaat agggttagtt gaaaaagcac gggattccat
     3241 cactcgaacg atcgcatctg ctgtgtctaa agaagtaaac aatggaatac catgttcaac
     3301 tgcttctctt cggatgatga aaccgtcttt agaagcattt ccccgatctt tatcaatcgt
     3361 attgacaacc acttgtgttt taccatctct tattaagtcg actacatttt tttctgctgc
     3421 ttcctcagaa attttcgcta ctgtttcaac gaccagacca tttttttcga aataagcagc
     3481 tgtatgtttc gttcctatca gactatagcc aatttctgta aaacgtttag ctaattctag
     3541 tgcttcttct tttgtttcgt ccgcaatcgt aaataatacg gctccatatt caggcaagtg
     3601 taatccagaa gcttcgaatg ccttgtacaa agctttgtct aattgtctgt cagatcccat
     3661 tacttctcca gtcgatttca tttcaggccc taagtaagta tcaactttct gcaatttcgt
     3721 aaaagaaaag acaggtgctt taacatgaac ttcttttgtt tctggataaa gtccgttttc
     3781 ataaccaagt gtctttagtt tctctcccaa aatagctttc gtcgcaattt gtgccattgg
     3841 aattcctgtc accttactta agaatggaac agtccgactt gctcgaggat tgacttcgat
     3901 cacataaacc tcattttcat ggatgacaaa ctggatattc atcatcccga cacaattcaa
     3961 acctaatgct aagcggatcg tgtaatcttc gatcgttttc ttgatcgatt cagataatgt
     4021 ttgaggtgga tacacagcca ttgaatcccc tgaatggaca ccagcacgct caatatgctc
     4081 catgatccct gggatgagta ctgtttcacc gtcacaaatc gcatccactt cgcattcacc
     4141 accaagcaag taacggtcca ctagtactgg atgttctgga gatgctttca cagcatgttc
     4201 catgtaatct tctaaatctc gctggttttc aacgatttcc atcgcacgtc cgcctaatac
     4261 atagcttgga cgaacaagaa ctggataacc aattctcgaa gcgataacca cggcttcttc
     4321 tttacttgta gcagtatccc ctaaaggttg aggaatatcc aactctttca gcgcttgctc
     4381 gaataaatca cgattttccg cacgatccaa gtcttcgatc gttgtcccta agatttttac
     4441 accagcagca gtcaaaggtt cggctaagtt gattgctgtc tgaccaccaa attgaacgat
     4501 tactcctatc ggctgttcta gctcgatcac attcatcaca tcttcgaagg tcaatggctc
     4561 aaaatataat ttatccgaaa tagaaaagtc agtcgaaact gtttcaggat tgctgttcat
     4621 gatgattgct tcgtatcctg cctgctggat tgctttgaca gaatgcacag tcgcatagtc
     4681 aaattcaacc ccttgtccga tacggatagg accagatccc agtactagaa ttgagggctt
     4741 ttctgatcgt gaactttcat tttcatattc ataggaacta taaaaataag gtgtttttga
     4801 ttcgaattcc gctgcacaag tgtcaaccat cttatataca ggaatcaaat cattttcttt
     4861 tctcatctgg cgaacggctt cttcgtttat ttcccacagc tccgccactt ttttgtctgt
     4921 aaaaccgttt tgtttcgctg ttttcaatat cttgtcatcc aacggctgct gtttcaactg
     4981 ctgttcgatt tctacgatat gcaacagttt atctaaaaag aataaatcga tttttgtcaa
     5041 atccgctaat tcttctatgg tgtaccctct tctaatagct tcagctacat aaaacaggcg
     5101 atcatcttgt gctcgaacca ctttttggat taggtcatcg tccgtgatgt ctgccaactc
     5161 aggaagttcg atatggtgtg taccgatttc taaagaacgc acagctttca ataaagactc
     5221 ttcgatattc cgaccgatag ccatcacttc accagttgct ttcatctgcg tgcctaaaag
     5281 ccgttctccg ttttcaaact tatcaaacgg ccagcgagga attttagcta caacataatc
     5341 taatgcaggt tcaaattctg caaaagtcgt tcctgtaaca ggatttttca tttcgtctaa
     5401 tgttaatcca agcgctattt tagcagctag cttagcgatc ggatagccag tcgccttact
     5461 tgctaacgca gatgaacgag aaacacgcgg attgacttca atcacataat aattaaaact
     5521 atgtggatca agagcgagtt ggacattaca gcctccctcg atcttaagtg ctcgaataat
     5581 cgaaagagaa gcatcacgaa gcatttgata ttcatggtca gacaatgttt gactaggggc
     5641 aaatacgata gaatcacctg tatggatacc caccgggtca aaattttcca tattgcagac
     5701 aacgattgca ttatcagccg aatctctcat cacttcatat tcaatttctt taaaacctgc
     5761 aatacttcgt tcaatcaaac attgggtagc gggggaaaga cttaatccat tttctgatac
     5821 gatacgtaat tcttcttcat tttcacacat acctccacca gttccgccta aagtaaaggc
     5881 tggacgaacg atgacaggat agccgatttt atttgcaaaa tctacagctt cttctactgt
     5941 attgacgatt tcagactcgg ggattggctg attcaattct ttcatcaact gtttgaatag
     6001 atcacgatct tctgcttgat cgatcgcaga aagctttgtt cctagaagtt ctatgttcaa
     6061 ttcttctaaa atacctgatg ctgccagctc cattgccata ttgaggcctg tctgtccgcc
     6121 taaagttgga aggagagcat ctggacgctc ttttcgaaga atccttgata caaattcaag
     6181 tgtgatcggt tcgatatata cttgatctgc cacttcttta tccgtcatga tcgtcgcagg
     6241 attagaattt actaaaacca cttcatatcc ttcttctttt aaggcaaggc aggcttgtgt
     6301 tcctgcgtag tcaaattctg cggcttgacc aatgatgatc ggaccagatc caatcaccat
     6361 aattttcttg atatctgtac gtttcggcat ctgtttactg ctcctttcct gcgtccatca
     6421 tttccataaa ttcatcaaat aaatgtagtg catcatgcgg accaggggca gcatctggat
     6481 gaaactgcac actgaaagcg ggataatctc tgtgtttgac tccttctact gttccgtcat
     6541 ttacttctac atgagttacg atgagttttt ctggatcaat cgtggcttca tcgactgcat
     6601 agccatgatt ttgagaagta aaatcaattc ttccggtcgc tacttctcgt accgggtgat
     6661 ttaatcctcg atgtccgaat ttcattttat aggtatctgc accgttggct aaagcaaata
     6721 actggtgacc gagacaaata ccaaaaatcg gtacttttcc ttggattttt ttgatcattt
     6781 cgattgcttc tggcacatct tttggatcac caggtccatt cgtcagcatg actccgtctg
     6841 gtgatagcgc taaaatcgtc tcggcatctg tgttataggg aagtactgtg aggttacagt
     6901 ttcttttact caattctcgt aaaatactat gtttcaagcc gaagtcaatg acaatgactt
     6961 tccgacctgt tcctggactt ggataaggtt tgttagttga gacttgctgc acttgattgg
     7021 ttgctagaac ggttgctttc agctgatcaa atgcatgttc gaaggaatct ccagcatcaa
     7081 ctatactagc tttcatcgtt ccgacttcac ggattttgcg agtaagggcg cgcgtatcga
     7141 tacctgatat tccaggaatt cctttttgtt tcaaaaatga atctagcgtc atttggtttc
     7201 gccagtttga agctacacgt gcatgttctt ttactactac tcctttgcat gttggagaaa
     7261 tcgattcata atcatctcta tttaccccat aattacccac cataggatag gtaaatgtaa
     7321 tgatttgacc gttaaaactt tgatcagtga tagtttcttg ataaccagtc atacttgttg
     7381 taaaaacgat ttctccgaag acactagcat ctgcaccaaa agcctttcct tcgaaaactg
     7441 ttccatcttc taatataagc aaacgttcca tacttttatt ctcccttctg ccacacaagt
     7501 tttccgttga caaaagtcat ttgtgtttcg ccttttactt tccagccgat aaaaggtgta
     7561 ttctcccctt ttgataaaaa gtcttcttta tcgatcgtgc aagtttgtgt aatatcaaaa
     7621 actgctacat cagcgggggc accaaccgtt agcgttcctg cattcaaacc aaaaatctca
     7681 gccggtttaa ctgccaacca attgatcact tgttccaacg tgaatcgacc agtttctaca
     7741 aaatgagtat agatcagttg aaacgctgtt tcgcttccta cgataccaaa tggtgatttc
     7801 atgaaacttt ggcttttttc ttctaatcca tggggggcat ggtctgtagc gatgcaatcg
     7861 atcgtaccgt caagcaagcc ttcgatcaaa gcttctctgt cctcccgtcc tcttagcggc
     7921 gggttcattt tccaaaagcc gaaatcttcg ggaatatctt cgtcgatcaa aataagatga
     7981 tggggtgaaa cttctgctgt tacgtggatt cctgcttttt ttgcgtctct gattacacgg
     8041 acactttctt tagtagaaac atggcaaaca tggtaatgaa caccagttgc ttcagctagc
     8101 aacagatctc tagcaatttg agaagactcg gttacgctta agatcccggg aagaccaagt
     8161 tcttctgcct tcttgccagc gtgcataacg ccgccaaata ataaagattc atcttctgta
     8221 tgggctacta gggctttatt gttttttgca gcttctttca tagccagata catcgtccca
     8281 gctgtttgga cacctacacc gtcatttgtg aaagcaaaag caccttcttc aatcagtgct
     8341 tcttgatcaa ctaatttctc acttcgcaga ttttctgtga ttggcgcata ctgcaatacc
     8401 ttcacaactg catcttttct aatgatgtca tatacttgtc tcagcttttc tgccgtatca
     8461 ggtacaggat ttaaattcgg catcgcgcat acagtagtga aaccgccacg agcagctgag
     8521 cgagtcccag cttcgatcgt ctccttgtat gtaaatcctg gctctctcaa atgaacatgg
     8581 acgtccacta aaccaggtgt gatcaattgt ccctttgcat caaacacttc atcaaattcg
     8641 gcttctgaga atcctgtacc gattgctttg attcgcccat cttctatcca aatttctgct
     8701 ggtgtcgtca tattttttcg tgtattgatc tgaccgtttt taatgagtgt tttcattttt
     8761 tcatcctcct ataattacgc ttttccattt agcactgctt ctaagattgc cattctgacg
     8821 aatactccat tgctcatttg ctgaacgatc cgcgactgta atccttccac taatgaatca
     8881 gctaattcta catcacggtt aactggtgct gggtgcataa tgattgcatg tttttgcaac
     8941 ttcttcgcac gctcaactgt taacccatgt tcctgatggt agccttcttt tgagaaactt
     9001 tcttttccat catggcgttc gtgttgcacc cgaagcatca tcatgacatc aacttgatct
     9061 agtagttcat ccaatggcat ataatgtccg tacacttcga attcatcgtc gtaccattct
     9121 ctaggacctg agaagaatac ttgtgcgcct agacgtttta acatctgcat attggattta
     9181 gctacgcgag agtgagtgat atcacctacg atggcaactt tcagaccttc aaaatgacca
     9241 aattcttcat aaatggtcat caaatcaagc aagcattggg ttggatgctg tccgcttccg
     9301 tctcctccat tgataatcga acactggatt gttttacttt gtatcagttc gtcataatag
     9361 ttctcatcac catgacggat aaccgctacg tctacgccta atgctgacat cgtcagtact
     9421 gtatcgtata gtgtttcccc tttttggaca gagcttgtgc ttgcttcaaa ttcaatcact
     9481 tctagcccta gttttttctc cgctacatcg aagcttttat gtgtgcgagt actattttcg
     9541 aaaaagaggt ttgtagcaaa gtactggcgc ttttgcggag tccaattagc accttgcttg
     9601 aattcctgcc ctctgcggat caagcccata acttcttgat cagtaagtgc ctcaacggtc
     9661 aataaatgct ttaagctgat acgttctgat ttgatgatca taatgtttcc tccttagttt
     9721 tcttcgcttc gtgaagtttc tggtaagata agatttaaaa tgatgccaag tacagttgct
     9781 aatgccattg ccgacaatgt aaatgtgcct acttcaaaga cgagtccgcc aattcctact
     9841 actaagataa ctgaagcaat caacagattt ttctttttat caaaatcgat tttgttctcg
     9901 attaaaatct tcattccact tgcggcaatt actccaaata agataaaact gattcctgag
     9961 atgactggtc cagggatact taaaatcaaa gcgcttaatt ttcctacgaa tcctaaaatg
    10021 actgcaaata ctgcagctcc accgatcaca aacacgctat ggacacgtgt gatagccaga
    10081 acaccaatgt tttcaccgta acttgttaca ggaggacctc cgacaaaggc tgccacgatt
    10141 tgtgctaatc catcacctgt caacgtctta tgaagacccg gttcttcaaa gaaattacgt
    10201 ttagttagtt tattcaatac cataagatgg ccgatatgct ctgtcatcgt cacaaaagcg
    10261 atgggtgcca ttgttgtgat tgctccaaga tatagctttg gttcatattg tacaaaggga
    10321 atctcaaagt taggaacagc aaaccaggca gcgtctacga ttggcttagt atccacgatt
    10381 ccaaacagca aggctacgat gtatccgcta ataataccta gtaaaatcgg aatcaatccg
    10441 agaaaacctc ttagccacat gttgaagaaa attgttaatc ctaatgtgat cagcgctaca
    10501 gcaatatact tgaaatcata cgtgccctga ttatacatag catttgttgc tgcatttgcc
    10561 gcaagtccca acccgataac catgatgact ggaccgacga cgattggagg caagatttta
    10621 tccagccatg ctgaaccgat ctttttgatg atcagcgcaa caatcaaata gactaatcca
    10681 gttgtgactg ctccttgtgc aatcgctgga taaccatctg ttttcatcaa aagctgcatc
    10741 gctgcaatga aagcgaagct gcttcctaag tacgcaggta tctttccttt agtagtaaat
    10801 aagtacacca atgtacctag tcctgaactg actagcgcaa tacctggatc gatacctact
    10861 aagatgggga ctaacacggt tgctccgaac atcgtgaaca agtgttgtaa acttaatcct
    10921 acccaatgtc ctggggttgg gcgatcgtgg atatctaaca ccacgtcatc attatgaaat
    10981 ccttttttct ccgacatgtt tctctctcct ctaatcttcc tctttcaaaa tcatgatgcg
    11041 atcttgtcca tcaagctctt gcatctctac taaaatctct tccgcccgag atgttggaat
    11101 gttcttccca acataatctg cacggattgg caattctcga tgcccacgat cgaccaggac
    11161 tgctaatgaa atttttcttg gtctgccaaa atccattact gcgtccattg ctgctcgaat
    11221 cgttcgtcct gtatacaaaa catcatcaat caaaataact tcttttcctt ctaaggaaac
    11281 tgggatgtca gaagaatgaa gttccggttc ttctggattt tcttttttgt catctcggta
    11341 taaagtgata tccagttctc ctacaggaat atcgatatct tctagctgct tcaggcgttc
    11401 ggcaattctt gatgcaatgt agattcctcg tgttttgata cctaccaata cgatatcttg
    11461 gatactatgg tttctctcga taatctcata agtaattcga gtcaatgctc gcttcatggt
    11521 tacctgatct actacttctt ttgcttgcat tttcattccc cctacttttt tgcataaaaa
    11581 aactcctgcc ctaagaggac aggaggaatg tttagactga gcgtatactg atacactgat
    11641 agctatacta tgtcaatgag caatctactt atggcgcacc atacgtatcc ttgctcccac
    11701 ccttcttagc ctcacgggac taaacttaaa ggaattctat taagttttat gctttcgttg
    11761 ttaattacta taccgttatc taaattaaaa tgcaatgttt tttcttaatt tttctaaagt
    11821 tttttcaaaa acttctggaa gaggtgcttc aaagatcatc ttttcgtttg tagtcgggtg
    11881 cgtaaaacct aacatttgcg catggagaaa ctgaccattg cctttcaaag ttttcttcgg
    11941 tccgtatact ggatctccag ctaacggata accgatatac ttcatatgca cacggatctg
    12001 gtgtgttctt ccggtttcta gttttaattc aaccagagta aatccttcga aacgttccaa
    12061 tacagtaaaa tgtgtgacag ccgacttccc atcttctctt atcgcctgca ttttccggtt
    12121 ttccttcgaa cggccgattg gcgcattgat ggttcctttt tcgtgaggaa tctctccatg
    12181 gactaaagca acatatttgc gcaaagaggt cttatctttt aattgctgag ctaatgattc
    12241 gtgtgcctgg tcatttttgg caaccattag cagtccggaa gtatctttgt cgattcggtg
    12301 aacaatacct ggacgaataa cgccgttgat cgtagaaaga tccttgatat ggtacatcag
    12361 cgcattgacc attgtgccat gcgtatgacc tgctgaagga tgaacgacca ttccttgagg
    12421 tttgtttatc accgctacat cttcatcttc gtaaacgatc gtcaacggga tattttctgg
    12481 gaccacatct aatgtttctg gttcggggat caagacagta atctcgtctc cttgttttac
    12541 tttgtaattt gctttgactg gctgtccatt gacagaaacc gctccatttt tcagccacag
    12601 ctgaatttgc gaacgactgt aatcggtgaa taaattagtc aatactttat cgatcctacc
    12661 agattcatgg tgaatcgtcg ttttaagttc tgtcattttt ttctttgatc cttttcatct
    12721 aaaataatat aaattaacat acaaatcact ccacatacaa gtgtcacatc tgctacgtta
    12781 aaaatcggaa agctcataaa atctgtctgg aacatatcta ctacatagcc aagacgaata
    12841 cggtcgataa aattgccaat cgcaccagct agaatcaaac tcaatccaat catgaaccag
    12901 cgattacctt tttcacgatt tttaatcatc aatgtgagaa tcacaacgac tgcaatgagt
    12961 gtcactatag taaagaacca catcttccct tctagcatac tccatgcggc tccagtgttt
    13021 cgaatatgat tcagtgacaa aatacctggt ataagatttt tcgtctctcc aagcgctaga
    13081 ttatcagtga tccaccattt gaccaactga tccactccta ctagcaaagc gctgaatatc
    13141 caataaataa ttaacaaaaa atcaatcctt ttctatttgt ttataaatat cacgaggtcg
    13201 tacgatcccg agcagattac catcgggatt cccatcttta gtgataagta cggcttccaa
    13261 agtcccttgc tcacgaaaca tcgcttcaac ctcgtaaata tgtgttttag ctggaacaaa
    13321 atgataattt gtgtactttc cgttttggat gatcaaatcc aacgctcgtt tatttgttaa
    13381 atcaaccgga cctttttggc tttcttttgc tagccaaaac ccgatattac gtaaagtaat
    13441 caatgctaca aattttcctt tattgtataa cggaaattgt gaataccttt tttctgctac
    13501 tatctttaac agttcaagta acggaagatc ttgttcaaaa cctgttacac gttttgcaaa
    13561 tcgaggcagc acggtttcag gctgaatcag ttcttgttcg atcagctgga ttcgcttgac
    13621 tgtccaatca tttggctctg caatgacaaa atcgactgcg atccgatcat ggacgatcgc
    13681 attccttaac tgagcaagct gtaaaagatc atcttcgtat tttctgatca ttaattgttt
    13741 tctttgagct aggcgtctga caagttcggt aaatcccata tttcgtgcat ttcccatctc
    13801 gtctctcatc catttttcga ttcgattgaa actgattaaa aaaagatcag agtttgccat
    13861 aattttcctc cttgatcaaa tatgcccggt tttttatata gaaaaagaac gcaatcaaca
    13921 caataactgc aaaaaaactg aaaaataaat tttgtaggaa aacggccccc aacccagttg
    13981 ctagaaataa aaacggatag atttgaagaa tcaagtaatt cattgtgatc acataagcag
    14041 gctttgcttc tttcactgta taaacggggc tttttagcca ataaaaggtg acctgcatcg
    14101 taaagggcac ttccagatca agcgttatct tttgttcatg actgagaaca ctgcgttttt
    14161 cattgttgat tcttagctga atcgagctac ccataccgta aaaaccggtt tttcttataa
    14221 atgtcgtttt catcataccc tcctattatg ctttagtata gctcaaaata caataaagaa
    14281 aaagaatgat cctagtgtaa acaaaattaa ttccctatcg tctaaaaaaa gaagctgtag
    14341 tcaaaataca gctccttcga tggtagatta taatcaagct taaaacaatc ccaacacttc
    14401 tccgtcattt gtcacgtcca tattcaatgc agcaggtttt ttaggcaaac caggcatcgt
    14461 cataacatct ccagtcaagg ctactaaaaa tcctgcccct aattttggga tgatttcgcg
    14521 gatcgtgatg gtgaaatctt ccggtgcacc tagcagtgaa ggatcatcag aaaatgaata
    14581 ctgtgttttt gccatacaaa tcggcagttt gtcccatcca ttcttcgtaa actcttctat
    14641 ttgtttttgc gcttttttgc tgaaaacaac accatttcct ccgtaaattt tcttgacgat
    14701 tgtttctgtc ttttcttgta tcgtttcttc taggcggtat aatggcttgt aattagcttc
    14761 ttttctatca atcaatcgta ctacagcttt tgctaaatca acgccgcctt ctgctccatc
    14821 tgcccataca ctcgctctct tggcaagaat cccctgattt tcacacaagt gctccaacag
    14881 tgtgagttca ttgtctgtat ctgttacgaa ttcattgatt gcgacgatca caggtagctg
    14941 atattgttcc atattacgta tatggcgctt taaatttgca aaacctactt tcaaagcatc
    15001 aagattctca tttttaagtt catctttttt cattccgcca tgcattttca atgcacggac
    15061 agtggcgaca atcaccacag catccggcgc ttttttcaag ttaggtactt tgatatctaa
    15121 aaatttctct ccgccaagat ctgcaccaaa tcctgcctct gtgacaacgt aatctgctaa
    15181 tctcaatgct gtcttggtag ctaaaatact attgcagcca tgagcgatat tggcaaacgg
    15241 tcccccgtgg acaaaagctg gtgttccgta aattgtttgt acaagattcg gcttgattgc
    15301 atctttcagc agtaatgcca gagcgccttc tgctttcaag tctccagcag tcacaggctg
    15361 acggtcaaat gtatagccga caacgatcct tgcaagacga gcctttaaat cttcaaggtc
    15421 tgccgcaaga cacaagatag ccatgatttc gctggcaact gtgatatcaa acccatcttc
    15481 tcttggaaca ccttggattg gcccacccaa gccaacaatt actttacgca gttcacgatc
    15541 attgagatcg actacccgtt tccaaatcac tcggcgggca tcgatgttca attcatttcc
    15601 ttgatgaata tgattatcta aaagagcaga taatgcatta ttagctgttg tgatagcatg
    15661 catgtcacca gtaaaatgca agttgatatc ttccataggc aatacctgtg catatcctcc
    15721 gcctgcagca ccacctttga ttcccatcac aggaccaagt gagggttctc gcaatgcaat
    15781 aacagatttt ttattgattt tattgagcgc gtctcctaac cctacagtga tcgttgactt
    15841 tccttcgcca gctggtgttg gattgatgga agtaactaag atcaaatgcc cctcagcgtt
    15901 atcctttgat cgattgattc cagaaaaatt gattttcgcc ttatatttcc catacagttc
    15961 caaatcgtct tccttaagat ctaatttact agccacttca acaatcggtt ctagctctgt
    16021 ttcttgtgaa atttgcagat cattcttcat ttgattaccc cttttcacga actttattca
    16081 acaaaatatc ttctttgtta agatatgcca attatacctt tttttattca gaaaaaaaac
    16141 ataaaattac aggtttttaa tttatttgac gtacttataa atactcttta taatgaaggc
    16201 tatgatcaga ctttagaaag gtcgtgagtg ctttgaccac aggaattgta aaatggtttg
    16261 acaacaaaaa aggatacggc tttatcagtt atgatgacac agaagaaatt tttgtacatt
    16321 ttactgcgat tgaagaagag ggattcaaaa ctttggaaga aaatcaagtg gtcgaatttg
    16381 agatcatcga agggaatcga ggaacacaag ctgcccatgt caagaaaggc aacgttcaga
    16441 ctaccgatac tagtatgcat gaatagataa acaaatagag tgagacgcta aacgggatga
    16501 accgtacgtc tcactctttt cttttcctgt aaaaattcgt ttttttaggt tctgattttt
    16561 aaaattagct ttttcttttt ttcgtataag gataaggaat tgattctgat ttgggtccgt
    16621 ttttttcaaa atgatcaaaa aggactactt gatctttttt tcgcatcatg aaaacagatg
    16681 gggctttctc atcttgcaac atcatttgca ccccagtatt agttggcgtc aaagatcgaa
    16741 tctgtccgac agatatttct cggtctttcc aaa
//
LOCUS       DAYYFD010000061        16714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000061 DAYYFD010000000
VERSION     DAYYFD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.061"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACHA25_001901"
     CDS             complement(251..1462)
                     /locus_tag="ACHA25_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8763191.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACHA25_001902"
     CDS             complement(1581..2216)
                     /locus_tag="ACHA25_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8763192.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="ACHA25_001903"
     CDS             complement(2352..3005)
                     /locus_tag="ACHA25_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763193.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="ACHA25_001904"
     CDS             complement(3108..3314)
                     /locus_tag="ACHA25_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM8763194.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="ACHA25_001905"
     CDS             complement(3390..4136)
                     /locus_tag="ACHA25_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM8763195.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="ACHA25_001906"
     CDS             4296..4520
                     /locus_tag="ACHA25_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM8763196.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="ACHA25_001907"
     CDS             4544..5305
                     /locus_tag="ACHA25_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8763197.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="ACHA25_001908"
     CDS             5317..5574
                     /locus_tag="ACHA25_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763198.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="ACHA25_001909"
     CDS             5666..5917
                     /locus_tag="ACHA25_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM8763199.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5893..6066
                     /locus_tag="ACHA25_001910"
     CDS             5893..6066
                     /locus_tag="ACHA25_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763200.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6180..6587)
                     /locus_tag="ACHA25_001911"
     CDS             complement(6180..6587)
                     /locus_tag="ACHA25_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763201.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="ACHA25_001912"
     CDS             6748..6972
                     /locus_tag="ACHA25_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763202.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="ACHA25_001913"
     CDS             6982..7176
                     /locus_tag="ACHA25_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763203.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="ACHA25_001914"
     CDS             7179..8201
                     /locus_tag="ACHA25_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8763204.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="ACHA25_001915"
     CDS             8164..8979
                     /locus_tag="ACHA25_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM8763205.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="ACHA25_001916"
     CDS             8997..9779
                     /locus_tag="ACHA25_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763206.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="ACHA25_001917"
     CDS             9779..10627
                     /locus_tag="ACHA25_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8763207.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="ACHA25_001918"
     CDS             10624..10866
                     /locus_tag="ACHA25_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763208.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="ACHA25_001919"
     CDS             11009..11377
                     /locus_tag="ACHA25_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8763209.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="ACHA25_001920"
     CDS             11392..11553
                     /locus_tag="ACHA25_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8763210.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="ACHA25_001921"
     CDS             11550..11876
                     /locus_tag="ACHA25_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763211.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="ACHA25_001922"
     CDS             11873..12688
                     /locus_tag="ACHA25_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM8763212.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12690..12815
                     /locus_tag="ACHA25_001923"
     CDS             12690..12815
                     /locus_tag="ACHA25_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763213.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12827..13138
                     /locus_tag="ACHA25_001924"
     CDS             12827..13138
                     /locus_tag="ACHA25_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8763214.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13135..13380
                     /locus_tag="ACHA25_001925"
     CDS             13135..13380
                     /locus_tag="ACHA25_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763215.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="ACHA25_001926"
     CDS             13377..13793
                     /locus_tag="ACHA25_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8763216.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="ACHA25_001927"
     CDS             13790..14128
                     /locus_tag="ACHA25_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763217.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="ACHA25_001928"
     CDS             14125..14355
                     /locus_tag="ACHA25_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763218.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="ACHA25_001929"
     CDS             14362..14565
                     /locus_tag="ACHA25_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8763219.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="ACHA25_001930"
     CDS             14562..14858
                     /locus_tag="ACHA25_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763220.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="ACHA25_001931"
     CDS             14935..15348
                     /locus_tag="ACHA25_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8763221.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="ACHA25_001932"
     CDS             15361..15747
                     /locus_tag="ACHA25_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763222.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="ACHA25_001933"
     CDS             16298..16525
                     /locus_tag="ACHA25_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763223.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..>16714
                     /locus_tag="ACHA25_001934"
     CDS             16522..>16714
                     /locus_tag="ACHA25_001934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763224.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5664 a         2726 c         3485 g         4839 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DAYYFD010000062        16644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000062 DAYYFD010000000
VERSION     DAYYFD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.062"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..178)
                     /locus_tag="ACHA25_001935"
     CDS             complement(<1..178)
                     /locus_tag="ACHA25_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8763225.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLY"
     gene            complement(180..377)
                     /locus_tag="ACHA25_001936"
     CDS             complement(180..377)
                     /locus_tag="ACHA25_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8763226.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(771..2498)
                     /locus_tag="ACHA25_001937"
     CDS             complement(771..2498)
                     /locus_tag="ACHA25_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8763227.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2491..3684)
                     /locus_tag="ACHA25_001938"
     CDS             complement(2491..3684)
                     /locus_tag="ACHA25_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8763228.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3871..4515)
                     /locus_tag="ACHA25_001939"
     CDS             complement(3871..4515)
                     /locus_tag="ACHA25_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8763229.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4609..4743)
                     /locus_tag="ACHA25_001940"
     CDS             complement(4609..4743)
                     /locus_tag="ACHA25_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8763230.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4745..6877)
                     /gene="feoB"
                     /locus_tag="ACHA25_001941"
     CDS             complement(4745..6877)
                     /gene="feoB"
                     /locus_tag="ACHA25_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8763231.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6874..7347)
                     /locus_tag="ACHA25_001942"
     CDS             complement(6874..7347)
                     /locus_tag="ACHA25_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8763232.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7748..7972
                     /gene="nrdH"
                     /locus_tag="ACHA25_001943"
     CDS             7748..7972
                     /gene="nrdH"
                     /locus_tag="ACHA25_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8763233.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8131..10290
                     /gene="nrdE"
                     /locus_tag="ACHA25_001944"
     CDS             8131..10290
                     /gene="nrdE"
                     /locus_tag="ACHA25_001944"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8763234.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10340..11305
                     /gene="nrdF"
                     /locus_tag="ACHA25_001945"
     CDS             10340..11305
                     /gene="nrdF"
                     /locus_tag="ACHA25_001945"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8763235.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11394..12533)
                     /locus_tag="ACHA25_001946"
     CDS             complement(11394..12533)
                     /locus_tag="ACHA25_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8763236.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12842..13555)
                     /locus_tag="ACHA25_001947"
     CDS             complement(12842..13555)
                     /locus_tag="ACHA25_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8763237.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13809..14510)
                     /gene="nagB"
                     /locus_tag="ACHA25_001948"
     CDS             complement(13809..14510)
                     /gene="nagB"
                     /locus_tag="ACHA25_001948"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8763238.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14744..16276
                     /locus_tag="ACHA25_001949"
     CDS             14744..16276
                     /locus_tag="ACHA25_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8763239.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16467..>16644)
                     /locus_tag="ACHA25_001950"
     CDS             complement(16467..>16644)
                     /locus_tag="ACHA25_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8763240.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5168 a         3428 c         2876 g         5172 t
ORIGIN      
        1 aatatagata attcaccaat ctatttatta aaggactggg atctaattca tatttttgtt
       61 tttctaattt taaatatacc ttttgtaaaa cctcaagaat atcagaatat ttaggatctt
      121 tacttataga tatatcatta tataattcat gaacaatttg cttagcattt tttttcattt
      181 tagcacttcc ctggaattgc tccacctaaa aaaccaccta tagacattcc tgcaatacaa
      241 gtagttgcct tggcccaatc gaccgtacat ttatttttag tgcaatacac tccatttcca
      301 taatattttc cactatgagt ggtaccccca tagataagtt gtgtctcttt aatagacaaa
      361 atttttaaat gtttcataat ctaatcaccc ctaaattaat gtttttagac tattattcta
      421 acattcatta aatttcaaaa aacaattcct tcttgaaacg tagcaaaata attcctgaat
      481 aagaatttaa tcacttttaa gtatataaac tatgtatatt ctttaatatt ttttgagaat
      541 gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt
      601 tgactcgcac ccataaattc ataatagttt gtcggcgtct ttgaacgttt cgaagatgag
      661 ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt
      721 tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc
      781 cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct
      841 tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg
      901 tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg
      961 atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg
     1021 gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct
     1081 tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag
     1141 ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt
     1201 cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag
     1261 gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga
     1321 tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca
     1381 tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt
     1441 acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta
     1501 tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct
     1561 ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt
     1621 ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt
     1681 gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc
     1741 ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc
     1801 atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg
     1861 atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg
     1921 aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa
     1981 cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata
     2041 ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca
     2101 aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt
     2161 accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa
     2221 tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt
     2281 cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga
     2341 gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt
     2401 aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag
     2461 tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc
     2521 tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac
     2581 aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc
     2641 tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct
     2701 agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc
     2761 catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga
     2821 ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg
     2881 atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc
     2941 acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg
     3001 taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag
     3061 gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc
     3121 gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg
     3181 catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc
     3241 taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc
     3301 gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat
     3361 attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc
     3421 tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa
     3481 tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc
     3541 aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg
     3601 ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa
     3661 atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt
     3721 tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga
     3781 aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc
     3841 aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa
     3901 aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc
     3961 cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat
     4021 aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat
     4081 tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt
     4141 cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt
     4201 ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg
     4261 caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac
     4321 ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc
     4381 attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt
     4441 ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt
     4501 tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct
     4561 ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat
     4621 ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa
     4681 acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc
     4741 catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct
     4801 gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg
     4861 ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta actaaataag
     4921 ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat
     4981 ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat
     5041 aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg
     5101 atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca
     5161 atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa
     5221 ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat
     5281 aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg
     5341 cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag
     5401 gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg
     5461 tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag
     5521 gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact
     5581 ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt
     5641 caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa
     5701 tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc
     5761 tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg
     5821 gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca
     5881 tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac
     5941 caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat
     6001 aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa
     6061 tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg
     6121 tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt
     6181 ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta
     6241 tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt
     6301 tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg
     6361 gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt
     6421 cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa
     6481 gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg acatccatca
     6541 tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat
     6601 ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca
     6661 aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta
     6721 ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta
     6781 cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt
     6841 tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa
     6901 gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat
     6961 tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat
     7021 atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc
     7081 tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac
     7141 aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat
     7201 ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt
     7261 ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt
     7321 ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat
     7381 taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat
     7441 tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg
     7501 aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat
     7561 tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa
     7621 aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga
     7681 cttttttact aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc
     7741 actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa
     7801 acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga
     7861 agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc
     7921 agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta
     7981 aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc
     8041 aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca
     8101 tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt
     8161 tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag
     8221 gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt
     8281 ccatctatta tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc
     8341 attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat
     8401 ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc
     8461 aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac
     8521 tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga
     8581 tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg
     8641 tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct
     8701 gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa
     8761 gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa
     8821 ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg
     8881 gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat
     8941 gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt
     9001 tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa
     9061 gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca
     9121 aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa
     9181 ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat
     9241 ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg
     9301 ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacagatat ctcatgtaac
     9361 ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt
     9421 acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg
     9481 atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg cttacacact
     9541 tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt
     9601 tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga
     9661 aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa
     9721 tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt
     9781 tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg
     9841 tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg
     9901 agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt
     9961 aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct
    10021 tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa cggcacaaca acacgtcgat
    10081 caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg
    10141 aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac
    10201 aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga
    10261 agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt
    10321 taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat
    10381 tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac
    10441 acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga
    10501 tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg
    10561 catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat
    10621 tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac
    10681 gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa
    10741 agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag
    10801 cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt attatttagg
    10861 aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt
    10921 acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga
    10981 acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga
    11041 acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct
    11101 acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac
    11161 tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga
    11221 cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga
    11281 cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata
    11341 aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca
    11401 cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc
    11461 aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg
    11521 caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc
    11581 gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct
    11641 aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac
    11701 aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc
    11761 gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc
    11821 aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat
    11881 aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag
    11941 aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat
    12001 ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt
    12061 gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct
    12121 aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat
    12181 ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt
    12241 aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc
    12301 aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg
    12361 aacatataat agataaaacc agcaacaagc aatggtaaaa aaattgccga aagcattttc
    12421 aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt
    12481 actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc
    12541 ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc gctgagattt
    12601 tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa
    12661 acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa
    12721 cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact
    12781 atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca
    12841 tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct
    12901 cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca
    12961 ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct
    13021 cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta
    13081 actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc
    13141 cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag
    13201 caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga
    13261 tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat
    13321 ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag
    13381 cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac
    13441 gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca
    13501 tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa
    13561 caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac
    13621 ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg
    13681 tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt
    13741 tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact
    13801 attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga
    13861 ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt
    13921 gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa
    13981 ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg
    14041 tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca
    14101 ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt
    14161 ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt
    14221 ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa
    14281 tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa
    14341 gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg
    14401 cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca
    14461 aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct
    14521 cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt
    14581 atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt
    14641 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
    14701 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
    14761 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
    14821 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
    14881 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
    14941 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
    15001 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
    15061 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
    15121 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
    15181 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
    15241 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
    15301 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
    15361 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
    15421 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
    15481 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
    15541 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
    15601 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
    15661 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
    15721 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
    15781 tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact
    15841 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
    15901 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
    15961 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
    16021 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
    16081 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
    16141 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
    16201 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
    16261 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
    16321 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
    16381 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
    16441 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
    16501 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
    16561 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
    16621 ataaagtaca taaacataaa taca
//
LOCUS       DAYYFD010000063        16164 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000063 DAYYFD010000000
VERSION     DAYYFD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.063"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            220..888
                     /locus_tag="ACHA25_001951"
     CDS             220..888
                     /locus_tag="ACHA25_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8763241.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1088..1408)
                     /locus_tag="ACHA25_001952"
     CDS             complement(1088..1408)
                     /locus_tag="ACHA25_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763242.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1445..2827)
                     /locus_tag="ACHA25_001953"
     CDS             complement(1445..2827)
                     /locus_tag="ACHA25_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8763243.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADQANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2983..4170)
                     /locus_tag="ACHA25_001954"
     CDS             complement(2983..4170)
                     /locus_tag="ACHA25_001954"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8763244.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFSELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4177..5208)
                     /locus_tag="ACHA25_001955"
     CDS             complement(4177..5208)
                     /locus_tag="ACHA25_001955"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8763245.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5254..6678)
                     /locus_tag="ACHA25_001956"
     CDS             complement(5254..6678)
                     /locus_tag="ACHA25_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8763246.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6853..7758
                     /locus_tag="ACHA25_001957"
     CDS             6853..7758
                     /locus_tag="ACHA25_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8763247.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7849..8526
                     /locus_tag="ACHA25_001958"
     CDS             7849..8526
                     /locus_tag="ACHA25_001958"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8763248.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8523..8693
                     /locus_tag="ACHA25_001959"
     CDS             8523..8693
                     /locus_tag="ACHA25_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763249.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8807..9703
                     /locus_tag="ACHA25_001960"
     CDS             8807..9703
                     /locus_tag="ACHA25_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8763250.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9758..11128)
                     /locus_tag="ACHA25_001961"
     CDS             complement(9758..11128)
                     /locus_tag="ACHA25_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8763251.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11366..12112
                     /locus_tag="ACHA25_001962"
     CDS             11366..12112
                     /locus_tag="ACHA25_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8763252.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12109..12948
                     /locus_tag="ACHA25_001963"
     CDS             12109..12948
                     /locus_tag="ACHA25_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8763253.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12949..13898
                     /locus_tag="ACHA25_001964"
                     /pseudo
     CDS             12949..13898
                     /locus_tag="ACHA25_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14039..14791
                     /locus_tag="ACHA25_001965"
     CDS             14039..14791
                     /locus_tag="ACHA25_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763254.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14810..15961
                     /gene="queG"
                     /locus_tag="ACHA25_001966"
     CDS             14810..15961
                     /gene="queG"
                     /locus_tag="ACHA25_001966"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8763255.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5250 a         3007 c         3245 g         4662 t
ORIGIN      
        1 atttcgtaaa acaaccgtaa cggttgtgta aaaatatcat tttagagcat aaaaaaaagg
       61 agtggatttc tccactccaa aaaagtctaa cttttggggg tcactacagt gtcacagtcc
      121 tttttttagt aaaaggatac atttcttcct tgtatattcc atgcgtgttc gtgagaaagc
      181 atatataatt taattaacag atagttaggg ggagaaaaaa tgattttttc aattacaatg
      241 aaactggtgt tcgggttgat cggtttatta ttagtggtac gtttgcttgg gaagaaatcc
      301 atgtcagaaa tcacaccgtt tgatttagtc tatacgcttg ttttaggtgg gatcttagaa
      361 gaatcaacct atgatgacaa tgtccatgta gggcatgtcc tatttgccat tgcgctttgg
      421 gcagtaatga tttatggtat cgaacgtatc gtccaaaaaa atgaaaaggt caatcgttgg
      481 gtcaaaggcg agccatctgt tctaatcaaa gacggcgtca tcaacatgac agagttgaca
      541 aataaccata tcgagatgga gcagttgcga gcaatcttaa gacagcaaga atgctttttt
      601 cttgaaaatg ccaaacatgt tatcctagag aatgccggtc aaatgagtgt attgaaaaaa
      661 tcagatgaag acaaagcttt atcgatcctg cttgtagatg aagggcaaat tcagcataaa
      721 gtgcttcagt ccaatcaatt gactgaagca tggcttatgg agaacttgaa gaaagaaggc
      781 tatgcagatg taaaacagct gatttatgtt gaatggtcag aagaaaaagg gctctacata
      841 ctaaataaag aagacgtcat gcatgaatct taccgaatag atggatagat agacagaggt
      901 tgtgaaatca gcgccttggc ttgttactca aaactaagcg actttttcac aacctctgat
      961 atctaatata cgttgttcca gaagttgctt cttcgaaaaa taagccaaaa ttccacaaaa
     1021 atttaagaag caattttcga aaaatccttt cttatttttt gaagttgatc acttctgtca
     1081 caacctctca tgtcacaaac catttttttg tttcctttaa ctcaatatac cgtacgtctt
     1141 cccacaatat cggctgatgg tcaatcataa agtaatcttc ataggcttca ccaatgaagt
     1201 gcccttcgac ctcatccaag agtcttccaa attcatcttt taaattgagc tgaatgatta
     1261 ctgctttacg agataaaaag gcttctgtca gtatactatc gatttcttgc aatgtcattt
     1321 gttgtttagg aagaggctgt ttgcgtgctt cttcttcatt tttttgatac cgttgactag
     1381 ttcttccatc gcaaaagcag tcccccattt taatccaaat ggccgatctc gatactcgtt
     1441 atagtcaatg aatttttttg ctgtccgtct catcttttcc accttctaat ccactcatac
     1501 cacctgcatg cccgccaacg agagaactac gtgcgatggc gcgtcctcct tgcaagagac
     1561 tatgcgcatg gatgattgat tgaaagccgt atttttttct aatcgtatcg acaacaaaat
     1621 caactttcgc ttcatttatc tgcttttcag gatcttcaaa aaggttaagc tgtaaacctg
     1681 tcgcgtatac gaggtcggaa cagttcacgc ctacacgccg aatatcctgg tagtggtaat
     1741 gctggtggaa aatggtcaat aaagcatctg caagatctct gctagcattt gtcgcttcga
     1801 ttttcatttg ctggtgaaac ccggtcttgc catttggatc aagatagcct aaagagtatc
     1861 ctacccataa gctgactact tgtgtttttg ctgcagatcg tctcaagcga gtgccgactt
     1921 ggtcggccat ttctttgata acgatctcga tttcttctcg tcttgtataa tctctatcta
     1981 ggatttgact atttccgata ctttttgatt ttggtgtata ttcttgtccc aaaaaacttc
     2041 gatcgatgcc ccaagcatgg gcataaagct gagtaccgat gacccctagc ttgcttttca
     2101 agtgataata atccgcgtga gctaaatcat aaacagaatg tatacccatt tgtttcagac
     2161 gttttgctgt tcgtttgccg attccccaaa agtcagtgat atctgagatt ttccataact
     2221 tttctggtac atcttcataa cgccattcag caaacatttg ctttccgtct tttgagccat
     2281 tgtctaaggc tagtttagca agtagcgggt tatcgccaat tcctatagtc gtgtagattc
     2341 cggtctgctc aaacacatct ttatggatca gtttagccat gtcgtacgca tttgttttac
     2401 caaagagtgc catgctattt gttacatcaa ggaagctttc atcgacggaa tagacatgat
     2461 ggttcgcttg gtctgcatat tttttataga tagaattgat ttctctgttc ttttccatat
     2521 aataggccat ccttggggca acaatatata aatcacttgg ataggggaag ggaagatctc
     2581 tggcacggct gatattggtg atgccgtaag ctttttttgc tgcaggactg gaagccaaga
     2641 ttaatccgct accccgtgtt tctgtcgaat cggacggata ggacatgact actaatttag
     2701 ttgtaagggg atcaagatcc ttttcaacgc actctacgga ggcgtaaaaa gatttgcagt
     2761 caatacataa aatatctctt gaaggttctt tttcataaac aaataccgga ttttgattcg
     2821 cccacataac aatcacctct tcaacacaat tagaacacac gttctctttc cgtgtcaata
     2881 aaaaacagca attttcaaat acgagcaaaa ggctcacatt tggaaattgc tgagaggtta
     2941 tttttatcta tttgttttta gctgagatcg ttgatttttt tatcaaacat gaacagcttc
     3001 tggatcgatc tttgttgtcc catttccaat gtgtgcaaca taaaattccg gagcatagcc
     3061 gacaactttc atgtattcat catagacatt gtttttgaag gcaggtatat tttcttcttt
     3121 gacaagagca atcgcacagc caccaaatcc agctcctgtc attcgtgcac ctaagacacc
     3181 ttcttgtttt tgagcagcat ctactagtgt atctaattcg atcccagtta cttcataatc
     3241 atagcgaaga gagtggtgag aatcatttaa cagttgacca aatgcttcta agttcccagc
     3301 ttctaattca gcttttgcct tcaaagtccg ctgattttca gaaacggcat ggcgagctct
     3361 gcgtatcaat gtatcatcgc cgatttggtc aatagttgat tcaaattctt cttcatctaa
     3421 gtctcctaaa gaagagatag gcaaagtttt ttgcaaacgt gccaaggctt cttcgcattc
     3481 gctacggcgt tcattgtatt ttgaatctgc caattcccgc cgcttattcg tattcatgat
     3541 gacgattgca taaccatcta gtttcactgg aaccatttcg tatttcagcg tatttgtatc
     3601 taataagatt gcatgatcta gctcactgaa gccgatggca aactgatcca tgatacctga
     3661 attgacaccg atgaattcat tttctacttt tttacctgtt tggactagtt ccaagcgatc
     3721 tgtttttaaa tcaaacagat cttctaatac aacacctact aataattcta atgaagcgct
     3781 actggaaagt cctgcaccat tagggattgt tccttccact aataactcaa acccatgatc
     3841 gattgagtgt ccagcttctt ttaatttaag gaccattcct tttacgtaat ttgcccagtt
     3901 tgctttttta tcatattcta gctcatctaa agtaaaacta atgacaccga tatcgtcaaa
     3961 gtttgtggaa taaactaata ttttattatc gtctcgaggt gctgctacac cttgcgttcc
     4021 ataagtgatg gaagcgggga agacatggcc tccattgtaa tctgtgtgtt caccaatcaa
     4081 attgatgcgt cctggtgaga agtattgtgt agttttttta ggtccgaatg tttcggcaaa
     4141 ttttgtgttt aagcttcttt tgatttccat gatgagttat tcctttcttg ttttgatttt
     4201 gaattctgtt actgtttcaa atactgatcc ggcttttaaa tgaatcgaac caaatgctga
     4261 gaagcgctct gcacctggag ctgtttgagt ctcaaatgtg atgccaccgt gatgtgccag
     4321 ttttcggttg cgcatttctg gtgtttctgt gccgaaattt gcagtgaaaa taaccacact
     4381 agaagcatct gtagcaatat ctacttggat tcttttatcg gggctagtca agcgagcggc
     4441 ttctttaccc aagccggttt cttttaagaa aaatggatga tcgattccgt caacaagatt
     4501 tttttgatcc aaatcactag caaaaacgga cgctaatttt ttaggtattt gaaaatcgaa
     4561 agcacttcct gcagcatttt cttttacccc tataggaata ctatctgttc gcaaaggagc
     4621 ataagcttcg ctattgagcc aaagctcatg ttgatcgatt gactgagaag catctcccgt
     4681 caagttgaag taaacatgat ttgtcggatt gaataaagta tcttggtcac tggtgccgcg
     4741 agaggtcact cgccagatat tatcttttgt caaagtatag cggatttcca catccattgt
     4801 acctggaaaa ccatgttctc cgtcgggact tgtagtagta aaaatgacag aagcttcatt
     4861 ttcgccattc aaaatgacat aagaccattt ttttaattca aatccaggag caccaccatg
     4921 cagattatgt ccagtttttg gatctgtggc taattggtaa gtttttccat tgagagaaaa
     4981 gcggccattt tcgattctcc ctgcggtgcg accgatagaa gcaccgatat acggatcttt
     5041 ttcgatatat tcttcagcgg tgtcaaatcc caaaactaat tctcgatcat ttgattttaa
     5101 actgacgatt ctagcaccta aatcagagat agaaagtgct agcttgtttt tgttctccaa
     5161 ggttatcaag tgataacctt ggccaaaatc tttttctgta atcttcattt tcaagatcct
     5221 ttcttaatgg gaaggaacta ctgcatcgtg aagttaatct tccattgtat cagctggagc
     5281 caatttgttt tccaatttct caacgatttg tgcatgtttt ttctctgtca atgtaatctt
     5341 ccaagcaaaa ataatagcgg agaaaatcat caaagcagca ggagcataga atgcataagc
     5401 tttgaaagtc gttacgccat gagcagtgat atcagcagca gtagcattac cagtcatccc
     5461 agaagcgata gcgacaaacc ctacgatccc gttagacaaa gcaccagcga ttttatctag
     5521 taaagggcga attgatagag taacagcttc gttacggata ccatttttcc attgaccata
     5581 ttcaacagag tctgtgatcg tcatcaaagc ggataagaaa atcaattgtt gagggaagta
     5641 gaacaatact agcccgatga taacgattgg caaacttgaa cctgcgatcg taaagaaaaa
     5701 gtaagaagaa atcatcattg cgatccctcc aaggtaaacg tagcgacgag agatgacgcg
     5761 tgtaagcatt gggaatagtg gaacagctac taaaccaagt aaggcagcaa ctagaccaac
     5821 gatccaaaat tcagttggtt ggccaagtac atatttgaag aagtagaata atacggcagt
     5881 tgttgctgca ttggcaatcg cataagcgat ataagcaagg gcaagccaca tcaactgatc
     5941 attttgagca actgctttga acacatcttt gatcccagct ttttcatttt gctgacggat
     6001 cgcagtatcg acttcttttg tgcccaaggc agtgataatc gcagtaccgc ctgaaataac
     6061 agcagcaatc acaccgaacc agaaccagcc tgaagcgcct tcaatatgtt tatgagtaac
     6121 aagataggta aagaaagtaa cgatagggac aacgactaga gtagtaccat ttgcacctag
     6181 agaagagccg aaacgagcaa acgtagcaaa tttttcacgt tcctttgttt ccgtactcaa
     6241 agctggaatc attgaccaaa aagcgatatc cttaaatgaa taaaaactat ccaataagat
     6301 gaaaacgatt gcaaataata tgataaataa agtcgtatta tgagtagcta agccaaagaa
     6361 attggtaaag attgctacca agagtacgga actaacaacg ccgccgataa ctaaccatgg
     6421 tttgaattta ccgtattttg tacgagtatt atcgatgatg ccaccaatca aaggatcaaa
     6481 aataatttca actaagcgga tacctacaac taagcttgta actgtagcaa tcatttttgc
     6541 attcgttgcc ttatctgttc ctgcgaacat ctggcttgtc acgaatacca taaaataagt
     6601 gcttaatgta acatagaatg tgtcgtgtcc aaaagcgcct aaagcgtagg acacacgttg
     6661 gatcattttg cttttcataa tctgtattcc tctcctgttc aatatataat actaagtaaa
     6721 ccggttacaa cgaataacaa agatctttta atgacttaaa cttaacaaga aatcgcttcc
     6781 tttttaatat atttctgtga cttttattta actttttgtt gcattatact aaaggagtaa
     6841 ggagggatag acgtggagca atcgattttt ttaaaaaagg aacatcaaca agtagaaaaa
     6901 gaattgtatt ttgatttttg tggtttctct aaaacgatgc cgtttcattc ttttggtcct
     6961 gctattcgca atgattacat cctgcatgtc gtgatggaag gcaaaggatc ttatcatgtg
     7021 aaaaatcaac aattccaatt gaaaaaaggt gatctgttct tgattcgtcc aggagactcg
     7081 acgttttact tatcagatgg agaagatccg tggatgtatt gctggctttc atttggtggg
     7141 agtgctgcag aagaaatcat tcggcagtcg cttttcagag aagaccaata cacgatggtc
     7201 tcttctgaca tacaaaaata tatcgacatt attttagaat gcatgaaaga tacggaagaa
     7261 accttggaaa atgaattaca gttaacagca ttaacctacc gttttttatc agtcctttta
     7321 aaagatggcg gaaaggtcag cggtggtgta caaaaggttc attcctcttt agctgttgaa
     7381 acagtcaaat acatcgaggc acattatgct gaaaaattgt ctgttgagga aatcgcccag
     7441 catttagcgg tcaatcggag ccatttgtcg cgagtattca aaaatcatat gggtaccagt
     7501 atcaaggaat atttgatcgg ggtacggatc aatcgagggg cgtttcttct ttctctgacg
     7561 gatgaatccg tggaaagtat tgcctatcaa gtagggttca atagtctagt cgtatttagc
     7621 cgaatgttca aaaagagtac cggagaaacg gctacgaact atcgtaaacg tatgaaaaac
     7681 gaaaatttta agaatattgc tttggatacc ttgaaaaaac agttggaaaa acaaaccgca
     7741 gtttcttgga caacctgatt agtctggtta aaatgacaag catcggcaaa agaaacttgt
     7801 ggcggttatg ctacactgta aagcgaagac taaaaaggag gaaaagggat gcgtacactt
     7861 atcatcatta gccatccaac gatcgaagaa tctttaaatc agcaattttt caaagaagct
     7921 tctgctggtt taccagctac ttggcatcat ttggaatcaa gttatccaga cggaaaaata
     7981 gatatagaga ctgaactaca gctcttggcc cagcatgatc ggatcatttt ccagtttccg
     8041 ttttactggt atagttctcc tgctcattta aaaatttggc aagataatgt cttggaacga
     8101 gcagcacatg tattaaaagg gaaagaacta ggaatcgtat tgacaacagg tgtggcggaa
     8161 aaagaatacc aagctggagg aaaagaagaa tatacgattt ccgaattttt gaggccgtat
     8221 cagcgaatcg caaataaatt ccatatgacc tatcttccgc cattcgtttt agcacaattt
     8281 atgtatttat cccaggaaaa acgttgggag aagctaattg cttatcagca gtatctctca
     8341 ttggaaggca aaccatcgct gactcagcgg attgactggt ttattcaaag agtacaggaa
     8401 aatcagaaaa tgcaggaaga agaatctgaa aaacaagcat atatcgtaga agcactgact
     8461 gaggcaaaag agcagatcga agacttgacc tttacactgc aagaaatgaa aggaacttca
     8521 ttatgacaaa tgattggctg atccaacaat tagatgaatt aagcgaaaaa tcagaatatc
     8581 aagaccgtgc gctcttatat gaaataaaga aactgatggc tgagatagac gaacgaacaa
     8641 tgcagctgca aggagaaatc gacggacaat cttggaatca tctcaactgg tgaaattttc
     8701 ttataaataa caaagataaa aatgtgcaca gcgacaatta tttgctacaa taagaatatg
     8761 aagcgtttga taacgctatt atacataaaa attggtggtg aaaaaaatgt atgatgaaaa
     8821 tgagtgggga gaaaatgttc ggatgacccg aagaagacag caaaaacagg cagctcgtaa
     8881 gcggatgatc atgattgggg cagcagttgc cgtgtttttg atcatagcag gaggagttgc
     8941 gggtctatat tttcatggaa aaaattcgtc agaagcgaaa agtacgacca taacggaaac
     9001 aacgacaaag aaaacagaga cgaaacaaac aagtgagtca tcaactaaaa caaaagagac
     9061 acaaacttct gaagaaaaaa cagcaaatgg tttgccagag caacttcaag caatcgtaaa
     9121 tgattattcg aaaaagatcg aagaaaagac gcctgcattg attgaagaat accagacaga
     9181 aattcaggga aatcaagagg gaatagctgg tttatcagca gttgctaacc aaaaagcacg
     9241 tgagcttcaa gcaatctccg acgaagggat cagaaaatta cgtgcagcgt atcaggcagc
     9301 agaaaataaa gacggtgtag acttagatac tttgatcaat caattatcgg ctaattatac
     9361 gaatcatgtc gctaagattt ccgatattta tttgcagact agcgcctctc ttcaagcaga
     9421 gtcaacaagc acacaagaca caacatcttc tgattctgag acagcagagt cggtagaagc
     9481 ttctgaaatg caagctcgaa caacacaaga cacaacagat tcctctgaaa ctaataccaa
     9541 cagcgcgact tcacaggctg cgacaaccgt agttttggaa ggagaaggtc caaatcaaat
     9601 agccgaacga actggcgtac cagtagaaac gatcttgtcg ctgaatggga tgagcatgga
     9661 gaattatttc ctgactcccg gtgagacgct aaaattgaat tgataaaagc aataaaaaag
     9721 aacgaagctt ccactaaggt gaagcttcgt tcttttttta ttttccggtt cttttttcgc
     9781 gtacaaagta atggatcgtc atgtaaagca agaaaacgag tccaataatc aatgagattc
     9841 tcgtttcagg attgataaac atgaaaatca atacgatagc taaaaaagca agtgtcacat
     9901 aattcgtata aggagatagt ggcattttaa aaggatgatc atgcatttta tctgcgtgct
     9961 tctttttgaa gcgaatctcg ctgatcaaaa tcactaccca aggaaccata ccaggcaaaa
    10021 tacttgagct gtatacgaaa acgaaaatat tttctgactg gttgatgaac attggtagaa
    10081 gataatttaa gatcagacca attaagattc cacctgagat tgctacgaca ggataaaaag
    10141 gcgcaccgtg acgagatagt ttcagaaatt ttttagacat ctttccattt atggcaagtg
    10201 tatagaccat acgacttgcg ctgaaaatcc ctgaattgca acctgataat gccgctgtaa
    10261 tcacgacaaa attaatcagt cctgcagcaa atgtaatgcc gactttagaa aaagtttgta
    10321 caaaaggtga accaatctca ctcaattgat tccacggata gatactcaca atgataaaaa
    10381 tagcaccaat ataaaaaatc aagattcgac cgatagtgga ctggacagct tcaatgatcg
    10441 tctttttagg attctctgct tcaccagcag tcattccgat cagttcgatt ccttgatagg
    10501 aagcaacaac gatcgataat gcaaagaaaa atcctttgac gcctccagtg aagaatccac
    10561 catttttcca aagatttgaa atccctactg cctgaccatg attaccaaat ccaaataaga
    10621 ttaccccaag acctgcaatg atcatcaaaa tgatcgttat gactttgatg atcgaaaacc
    10681 aaaattctaa ttcgccaaac gccttgacag aaataagatt tgccagcata agaaacaaga
    10741 cgacaatgat tccgggaata attgccggca aatcaggcca ccaataattc atataagatc
    10801 ctacagcaat cacttcactt attcctacga caatatattg aaaaatatta ctccaagcgg
    10861 tcaagtagcc agccacagga tggatgtact cgctcgcgta atttgcaaaa gaaccagtcg
    10921 aaggatcaac gtaaagcatt tcgcctaaag cacgcataat caaatacaaa aagaatcctg
    10981 caatcgcata ggctcccatc actgagggcc ctgtccaacg tatggttgag gaggcaccca
    11041 taaacagacc gacaccgatc gttccaccta gtgcgatcat ttggatatga cgggcactaa
    11101 gacttcgagt taacttttct tcttccatac aaaaactcct ttcgttgttg agtcaacaag
    11161 aaaaaataat caaacgtttc aacacatgtt tttcattatt aggttagcct tattgaactc
    11221 ttgatgtgat tctacagaaa attttcataa aatgcgagga aaattcgaaa aaaacttttt
    11281 catcattcta cttgaaaatg ctttccattt tagttaaact atatttaaat aaaaagttag
    11341 gtaaacctaa aaaggagtgt aagatttgga acgtacggcc attgtaattc aagacctttc
    11401 agccgcttat caagggaaaa ctgttttgaa aactatcaac ttgacgataa atccacaaaa
    11461 aattaccggt attatcggac caaacggtgc aggaaaatca acatttatga aatctttgct
    11521 agagttgata ccagctacta ctggtaaagt caccttttct ggacagtcgg taaactcagt
    11581 ccgtaaaaaa atagcctacg tggaacagcg aagtgaattg gatctttcct ttccaattga
    11641 tgtattaggc gttgtacttt taggaacata tccatcttta cgaattggac aaagacctgg
    11701 gaaaaaagaa aaagaacgtg caagacaagc tttgaaaaaa gtagggatgg aagaatatgc
    11761 caaaagacag atcagcgaac tatcgggtgg acagcttcag agagttttta ttgcaagagc
    11821 tctagcccaa ggagcagaat ggatcttttt agatgaacca ttcgtaggga ttgatgcgtt
    11881 aagtgaacga aagatctttg acatcttgca ggaattgaag aattcaggaa aaacgatttt
    11941 gatcgtccat catgatcttc ataaagtaga agaatatttc gatgaggtta ttcttttaaa
    12001 taaacagctg atcgcttccg gtccagtaca agaagtcttt acatcgaaga accttcaatt
    12061 ggcctatggt gagatcatca gacatttagt aaaaggagga gagaaaaaat gatccaatcc
    12121 tttattgatg gattaatgaa ttatcaattc ctgcaatacg ctttggtcac ttccatgctt
    12181 gtaggacttg cttctggagt catcggttct tttattattt tacgagggat gtctttgatg
    12241 ggagacgcta tctctcacgc agttcttcca ggtgtagcca tttcttatat gtttggtttt
    12301 agttacatct ttggagccac tgcttttggg atgctaacag cagcagtgat tggtttcgtg
    12361 actcagcgga gccgattgaa aaatgataca gcgattggaa ttgtctttag ctcctttttt
    12421 gcattaggga ttatcttgat ttcatatgcc cagagtgcga cagatttgta tcatatttta
    12481 tttgggaatg tcttagctgt tagggaaagc gatttgttgc tgacagcact cgttagtgga
    12541 atcgtcttga tatttgtctt tttcttttat aaagaattga agatcacatc attcgatccg
    12601 acaatggcaa aagcttatgg gctgaacaca tcgctgatcc attatctttt gatgttcttt
    12661 ttgacattag tggctgtagt cagtttacag acagtaggaa caatcttggt gattgccatg
    12721 ttgatcacac cagccgccac ggcttacttg ctaacgaacc atttactgaa aatgatcatt
    12781 acagctgcag gaatcggtat gctaagtgca gttgtcggtg tgtttttcag ttatagttac
    12841 aattggccat caggagctac gatcgtgtta gcatgtaccg cattttttat ccttgctttt
    12901 ttattttctc caacaaaagg aattttattt cagaaaggaa gcagatagat gaaaacaaaa
    12961 aaatcactct ttttaatctt ggcagttagt ttcttggtat tagccggttg cggaaagcag
    13021 gcttctgacc aagcggatga aggaagtaaa gaaaagttgt cagtcgtggc taccaattcg
    13081 atcttggcgg acatggcaaa agaggtcgga acagatcaaa tagatatcca cagtatcgta
    13141 ccagtcggaa cagatccgca tgaatatgaa gtactaccag aagacatcaa aaaggcaagc
    13201 gatgcagatg ttattttata caacggtttg aatcttgaaa caggtaacag ctggttcgat
    13261 aacttgatgg aaacggctaa aaaagaagaa ggaaaagatt attttgcagt tagcaaaaat
    13321 gtagaacctc tttatttaac tagcggtgaa gaacatacaa aagcagatcc ccatgcttgg
    13381 ctagacctat ctaacggaat aaaatatgtg gaggaaatcg cacgtatatt ctctgaaaaa
    13441 gatgcagaaa atgcgacact ctataaaaaa atgcagaagc atatgtggaa aaactaaaag
    13501 aattagatac acaagcgaag gaaagttttg cttctatcga agagaacaaa aaattattag
    13561 taacaagtga aggggcattc aagtattttt cacgagcata tgatttgcca gcagcttata
    13621 tatgggagat caatacagaa agtcaaggta cgcctgatca aatgaaagcg atcattgatc
    13681 agataagagc aaaagaagta ccggttttat tcgtggaaac cagtgtagac tcaagaagca
    13741 tggaacgggt agcaaaagaa acgggtttga aaatctatga taaactgttc actgattcca
    13801 tagcaaaaga aggagaacaa ggagattctt actatcagat gatgaaatgg aatattgaaa
    13861 cgatccatga aggtttgtca caaacaaaag aaagctgaaa agtgcaaggt ctagcttgag
    13921 gagcagacct tgcatttttt ataagagata tgaaaaaaaa ttatgctgtt tttcgtcgta
    13981 cagatacctt ttttgaagaa attccgttat agttagagta aggaaggaag gtgtggctat
    14041 gaattttaca cgtaaagata aatatacgat taaagaagaa gtctttcgat tgatcagcca
    14101 gcaggaaaat accgacaagg atcaagacct tcaatttctt gaagcagaat tagctaaatt
    14161 attcatagca aatcaaaatg agacaacaga accagatgaa gagctgtaca taggcaaatg
    14221 gttgtttgat gaatttcagc ctttagatga aaataccggt atcggttatt ttgtgttcct
    14281 ttctccagtc ggcttcacac ctggcgtatt agttaaacaa aacgacgaaa taaccgtcaa
    14341 agcatggcct gcattatttt cagtcatcaa agcagaagga gtaatcaaaa agatccagtc
    14401 ggcctattat aaaagtcaat ttattgccaa cagcaaatta atgaatatgt tttataatcg
    14461 tctagtagaa attgctaggc ctgggaaaat cccgcaaaca ttcacgtaca cacagcaaaa
    14521 aaatctctat gaagaagtat tgtattcccg cgatctgtct gagttttcgc catgggaaga
    14581 ggtaaggctc catgaactgt taagtacaaa accagtttca ttgaattttt ttaatgcatt
    14641 agataacaaa agaaaattaa tttcaacaaa ttattgggaa gtatttgctg caagaaaaaa
    14701 atggatggaa gaaaagaaga ttgccgatca gcagatccag tttgtccagc tgaaaaaaga
    14761 aaaactatat tatgggttat acagccgtta acagcaagcg aggaagacca tgacattaaa
    14821 agaagaaatc atacaagaaa gcaaacgatt aggaattgac aaaatcgggt tcaccacggc
    14881 ggaacctttt gattcattaa aagaatcgtt ggaagaacaa aaagcagctg gccatacttc
    14941 tggctttgaa catccaaata tcgatgagcg tctttatcca gaaatgacat ttgatcagcc
    15001 gaaatcgatc atttccattg cacttgcgta tcctactagg atccatgaag aagtgcctag
    15061 ggatgaaaaa cgcgggcagt ttgctcgtgc ttcttggggg atcgattacc atgatatact
    15121 cagagaccgt ttgaaccgat tgatcgaatt catccaaaca agagcagaaa aatggcaaaa
    15181 agaagaggaa tggcgtctag ctccgcaagt agatactgga gaactgatcg atgtcgctgt
    15241 agcacagcga gctggtttag gatttattgg acgaaacgga ttgttgatca ctgaagagtt
    15301 cggttcattt gtctatcttg gtgagatcgt tactaatatt gtatttgagc ctgataaacc
    15361 aggagaattt ggttgcggtg actgtatgcg ctgcgtgact gcttgtccga caaaagcatt
    15421 gcttggtgac ggaagaatga atgcccagcg atgtttgtct tatcaaactc agacaaaagg
    15481 gatgatgccg gaagaatatc gtaaaaaaat gcgaaatgtc atttatggat gtgatatctg
    15541 ccagttagtc tgcccttaca atcaaggaaa agattttcat tttcatgaag agatggaacc
    15601 aaaagtagac gaaatatatc caaaattaaa gccaatgctt tcgatgtcta ataaagactt
    15661 caaacagcaa tttgggcatt tagctggttc atggcgtggg aagaaacctt tgcagagaaa
    15721 cgcattgatt gctttagcta atctaggtga tcgaacggca ttgcctgaga tttcagaatg
    15781 tttaacagat gtccgccctg tcatcagagg aacagcagct tgggctattg gacgtctagg
    15841 aacaaaagaa ccggaaaaat ggcttgatgt attagatgaa atattagcta aagaaacaga
    15901 agaagtcgtt ataaacgaat tggaacatgc ccagaaatta ttgcggagaa agttaaaata
    15961 aaaaaatagt taacattatt caaagttttt gtccagtaat tgttgtaaca attactggtt
    16021 tttttgtatt ttttacattt ttttaagaga atttgataaa aatcaaaaat ttttttgctt
    16081 ttttttatgg attattgcag tgaatgttac agaatctgcg tttttcattc aaaaataaaa
    16141 aaacatagaa aaacgattac tttg
//
LOCUS       DAYYFD010000064        16082 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000064 DAYYFD010000000
VERSION     DAYYFD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.064"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1137)
                     /locus_tag="ACHA25_001967"
     CDS             complement(94..1137)
                     /locus_tag="ACHA25_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8763256.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1514..2479)
                     /locus_tag="ACHA25_001968"
     CDS             complement(1514..2479)
                     /locus_tag="ACHA25_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8763257.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2713..3558
                     /locus_tag="ACHA25_001969"
     CDS             2713..3558
                     /locus_tag="ACHA25_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8763258.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3749..4936
                     /locus_tag="ACHA25_001970"
     CDS             3749..4936
                     /locus_tag="ACHA25_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8763259.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4942..5298
                     /locus_tag="ACHA25_001971"
     CDS             4942..5298
                     /locus_tag="ACHA25_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8763260.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5300..5638
                     /locus_tag="ACHA25_001972"
     CDS             5300..5638
                     /locus_tag="ACHA25_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8763261.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5714..5970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6033..7067
                     /locus_tag="ACHA25_001973"
     CDS             6033..7067
                     /locus_tag="ACHA25_001973"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8763262.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7060..7746
                     /locus_tag="ACHA25_001974"
     CDS             7060..7746
                     /locus_tag="ACHA25_001974"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8763263.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7770..8618
                     /locus_tag="ACHA25_001975"
     CDS             7770..8618
                     /locus_tag="ACHA25_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8763264.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8815..9588
                     /gene="sufC"
                     /locus_tag="ACHA25_001976"
     CDS             8815..9588
                     /gene="sufC"
                     /locus_tag="ACHA25_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8763265.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9601..10887
                     /gene="sufD"
                     /locus_tag="ACHA25_001977"
     CDS             9601..10887
                     /gene="sufD"
                     /locus_tag="ACHA25_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8763266.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10884..12119
                     /locus_tag="ACHA25_001978"
     CDS             10884..12119
                     /locus_tag="ACHA25_001978"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8763267.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12106..12576
                     /gene="sufU"
                     /locus_tag="ACHA25_001979"
     CDS             12106..12576
                     /gene="sufU"
                     /locus_tag="ACHA25_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8763268.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12581..13972
                     /gene="sufB"
                     /locus_tag="ACHA25_001980"
     CDS             12581..13972
                     /gene="sufB"
                     /locus_tag="ACHA25_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8763269.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14070..15212)
                     /locus_tag="ACHA25_001981"
     CDS             complement(14070..15212)
                     /locus_tag="ACHA25_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8763270.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(15263..16060)
                     /locus_tag="ACHA25_001982"
     CDS             complement(15263..16060)
                     /locus_tag="ACHA25_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8763271.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         5220 a         2963 c         3381 g         4518 t
ORIGIN      
        1 tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca
       61 tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag
      121 taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc
      181 ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc
      241 aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct
      301 gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt
      361 tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc
      421 aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa
      481 ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag
      541 tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag
      601 aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc
      661 tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca
      721 aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc
      781 aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt
      841 tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa
      901 ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa
      961 agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag
     1021 ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa
     1081 gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg
     1141 atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag
     1201 tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat
     1261 aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga
     1321 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt acgacctctt
     1381 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta caaaaacatg
     1441 aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt ttcacaacct
     1501 attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg ctatgttctt
     1561 taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt gcaaacagac
     1621 attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg ttgaagtaat
     1681 gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag gctgcatagc
     1741 ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg aaatacaata
     1801 cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca aagtttttaa
     1861 gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc ggacgtgtcg
     1921 gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat aagaacgcat
     1981 agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag ttcccggcag
     2041 ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa attgcttcaa
     2101 ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg atcacttgga
     2161 agataacagc gcacagccaa atgaatttga caattggtgc aatactttca tatgaaacaa
     2221 aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag gcgatgattg
     2281 gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca aaaatgaata
     2341 tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg tgatagtctt
     2401 taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg atcggaataa
     2461 ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt ttagtaccaa
     2521 gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag cagacagctg
     2581 attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca tttctattat
     2641 agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat aaaaagttat
     2701 ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga aacaagaaat
     2761 ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa tcaaatcaaa
     2821 cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa agagatgctt
     2881 ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga agaatgggaa
     2941 gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg gatcgttgct
     3001 tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat caaaaggttt
     3061 cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga acaagtagcg
     3121 aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg cgtacaaaga
     3181 tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa attgtggctt
     3241 gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga agagatgaat
     3301 atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt gctgaaggaa
     3361 ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc aatgactccg
     3421 ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc tgatcagctg
     3481 ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc tcacagcgag
     3541 gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat acactaaaga
     3601 taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga aagttaattt
     3661 ttcccaaatg tctatggaaa gactttccat tatctaactg ggccgctata ataatcagca
     3721 gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa
     3781 tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg gtctgctctc
     3841 attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag gcgtagggat
     3901 gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc atttgaattc
     3961 caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg taatgggact
     4021 cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa actggtttcg
     4081 attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca tcttgatgat
     4141 ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga aatcggactt
     4201 ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga tcatggccca
     4261 agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt tcctgatgtc
     4321 tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg
     4381 aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag ttggattcaa
     4441 atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata cgtcaggaat
     4501 gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt ttggtaaagg
     4561 attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt ttaccgttat
     4621 cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt ttattttaat
     4681 ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct atatcgcaag
     4741 cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa atatcggatt
     4801 acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct cgattttagg
     4861 gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag cacctgcaag
     4921 atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg ttcaacatgc
     4981 aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt tgatatgatc
     5041 aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca agtaccaatc
     5101 cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa agatcaagta
     5161 ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat gctgatcaag
     5221 cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga aaaaatttat
     5281 gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca acctttggat
     5341 cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag aagaacttgg
     5401 agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct
     5461 gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt
     5521 ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat
     5581 gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg aattataaaa
     5641 tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa tcataagcaa
     5701 tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg tttgaagaga
     5761 gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta gagcagagta
     5821 agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt atacggaaag
     5881 aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa tcaaggtggt
     5941 aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag gcttttttgt
     6001 ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt aatccaacta acgaatgtca
     6061 aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt tcactaaacg
     6121 ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa agtacattag
     6181 tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta aacgatcaag
     6241 atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc ggtatgatct
     6301 tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag ctgccgctgg
     6361 aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg ttgcgtttag
     6421 tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga cagaaacaac
     6481 gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt gatgaggcta
     6541 ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta gcgatcaata
     6601 agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct
     6661 gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac gtattgactg
     6721 tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac atcgaaccag
     6781 aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg
     6841 caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa gcgattcgac
     6901 gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct
     6961 ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa gcagaagtgt
     7021 tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag cttttcagaa
     7081 acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat ccttgataca
     7141 ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt tttgggattg
     7201 atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta tggaatcgta
     7261 tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt tttgatcatt
     7321 ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat tccagctctt
     7381 gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc
     7441 cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat catctggaag
     7501 gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac aacgatctcg
     7561 atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg aggtcttgct
     7621 tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac tttaattatt
     7681 ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa aacagacaaa
     7741 agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga tttgcagcag
     7801 cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc aaagattcag
     7861 caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg cacgcagaga
     7921 ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat
     7981 tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat gccaattact
     8041 tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg
     8101 ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg
     8161 aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg ggacgcatca
     8221 tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat ctggaaacag
     8281 caacattcga tgatattgaa gaaaaaccta agaacttgaa gttcaaccat acgatcgacc
     8341 catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca atcaacgcaa
     8401 acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt gaaaaggaca
     8461 actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt gacaaaatca
     8521 aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca gacaaatggg
     8581 gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa aaaagatgag
     8641 aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat ttagaaatca
     8701 aaattgttta ataataaata aaagatattt gattttagta aggaaggtca ttgcgcttca
     8761 ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata atgcatgtct
     8821 gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct taaaggcgta
     8881 aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg cacaggaaaa
     8941 tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga aggcgaaatt
     9001 ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg acttggctta
     9061 tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga attcatgcgt
     9121 gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc atttttgaaa
     9181 aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga acgttatctg
     9241 aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt gttgatgtta
     9301 gaacctacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga tgctttgaaa
     9361 gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt gatcatcacc
     9421 cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat gatggaaggc
     9481 cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga aggctacgct
     9541 ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg gggagcaaga
     9601 atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc tttaagtaaa
     9661 ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc agatgaactg
     9721 ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga tgtgaaagaa
     9781 acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa agacaatcct
     9841 gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga attagcagaa
     9901 aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga actagtccaa
     9961 gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc actccatgta
    10021 gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat cgatgaacca
    10081 ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa gcatgtgttg
    10141 atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac aaccaaagaa
    10201 caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc tggttcaaaa
    10261 atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat gaatcgccgt
    10321 gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa tgacggtcat
    10381 gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga agtcaaaatc
    10441 gtggcaatca gctcgggcag acaaatccaa ggaatcgata cacgagtaac caataaagcc
    10501 cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg aacattgacc
    10561 tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca acaagaaagt
    10621 cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct tttgatcgat
    10681 gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc agaagaaatg
    10741 tactatctga tgagtagagg attgcataaa gaagaagcag agcgcttagt gattcgagga
    10801 ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga attagtcgaa
    10861 gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat gattttccta
    10921 tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca gcgacgaccc
    10981 aaaagccaaa acaagtactc caggcattag cgtactatta cgaacacgat aacgccaatg
    11041 ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa caagcaagag
    11101 aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt actcgtggta
    11161 cgacgacaag cttgaattgg gtagccagaa ccttcggcga ccgctatatc aaagcaggag
    11221 acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg cagcagttgg
    11281 caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc ttcctggata
    11341 tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt gctcatgtat
    11401 ccaatgtctt aggtgtcttc aatcctgttc aagaattagc tgaacttgtc catgaaaacg
    11461 gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catggccatt gatgtccaag
    11521 cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca acaggtattg
    11581 gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa tttggtggcg
    11641 aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct tggaaattcg
    11701 aagcagggac accaaatatc gccggcgcta tcgcattagg ccatgcaatc gattatttgc
    11761 aaaaactcgg tatggacaat atccatcgat atgaagaaga actagccgct tatgtgttgc
    11821 caaaattaca agcaattgat ggattgactg tttatggtcc acaagacccg aattttcgga
    11881 caggagtcgt tgcattcaat ttggatgggt tacatcccca tgatgtcgcg acagctttag
    11941 acatggaagg ggtagccgtc agagcaggtc atcattgtgc gcaaccgcta ttgaagtacc
    12001 ttgaagtatc agcgactgct cgcgcgagct tttatttcta caacacaacg caagacgccg
    12061 accgtttagt tgaagcaatc atggcaacaa aggagttttt ccaacatggg actatctaaa
    12121 ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccgca tcatcgtgga
    12181 agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg agatgtgatc
    12241 gaattgcaag tcgatgtaaa agacggaatc atcaaaaata tcgcctttga cggcagtggc
    12301 tgctccatca gtacggcaag tgcctcgatg atgacagacg ccgtcatcgg aaaatcaata
    12361 gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa agaagtcaaa
    12421 gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt tcccgctcgg
    12481 atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa cgatggtacc
    12541 gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac cagaattaga
    12601 agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag gagaaggctt
    12661 gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat ggatgcttga
    12721 attccgtctg aaatctctag aaacatttaa caaaatgcca atgcaaacat ggggccccga
    12781 cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg acaaacccgc
    12841 tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga ttggtatacc
    12901 agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag aagtcgtgta
    12961 ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca ctgactctgc
    13021 tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac cgccaaccga
    13081 caacaaatta gcagcgctga attcagccgt atggtcaggt gggaccttta tttacgtgcc
    13141 aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg cggaaaacac
    13201 ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc actatgtcga
    13261 aggttgtacg gcacctactt attcaagtaa cagcctgcat gccgccatcg tagaaatctt
    13321 tacaagaaaa gatgcgtatt gccgttacac aacgatccaa aactggtcag acaatgtgta
    13381 caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat ggatcgacgg
    13441 aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg gaaaaggtgc
    13501 acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag atacgggtgc
    13561 aaaaatgatc cacaatgcac caaatacatc tagctctatc gtctctaaat caatttcaaa
    13621 agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca gtgcgggttc
    13681 gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat ccgatacgat
    13741 cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca aagtctcaaa
    13801 aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat ctgaagcaac
    13861 tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa tggaatatgc
    13921 agtcgaactc aatcgattga tcagttatga gatggaaggg tctgtaggtt aataactatt
    13981 tcattgctcg aaacgttgat attaaagcgt ttcgagcgtt tttttatttg tattttaatg
    14041 gttggctacg tttttggcta cgtttggaat tattcgccca taaattttgc aaatcggtca
    14101 ccagttttct taacagattg tggtgtgaca tgagcataga tattcatggt agttttaata
    14161 tctttatgac ctaatcgttc ctgtacttct tttatcgacg caccactttc aaacaataag
    14221 ctacagtggg tatgcctaaa tccatgtgga gtaatttgag gctccatcgg tgtctttttg
    14281 taaatccagt ctaaccaatc gtttactact tgggggtagt acaacttgtt tgtgtttgta
    14341 gtgaaaagaa actgttttgg tgaattcgtg ttatagccat ataagatcat tattttcatt
    14401 tgttcttgtt gccaaatacg cagttgttta agtgtaaaat catcaagctg aatcgttctt
    14461 gatgaatttt tagttttagg tacttgtaga actacttgat taaactcgtc ttgagcgata
    14521 gttttaccaa ttgttattgt tttattgaaa tagtctatat cttcccattg aagcgccaat
    14581 gcttcgcttt tacgcattcc tgtataagct aataaacgga aaaaagtgta cagtttaact
    14641 gaattatttt ctttagtatt ctgtaaaaac agttgtaatt gatctttcgt gtaaaacttc
    14701 aagggttgtt cttcttcaat ttttcttgga agcaatgttt tagacatagg attatcctta
    14761 cagacttcca ttgctactcc aaatttaaat atttgagcag ttgtttttct caagaaacca
    14821 tattgtttta atgttttgtg ccattcattc acctgttttt ggcaatagct aacacttatt
    14881 ttatccaatc gtaattcccc aaaagcaggt aatatatggt ttttgcaaaa acgttgagca
    14941 acggcaacag aagacggttt tactcttgtt ctgtagttat caatccataa ttcatacaac
    15001 tcttgaaaag ttgtaacatt gattttaaac tcttgttttt ctttaaagtc tacaagtagt
    15061 ctattaagtg ctgtttgcgc ctcagctttt gatttgaatc ttctacgctt tgtacgtata
    15121 tcttttccag tcttgggatc tttccctaaa taagtttcaa atccccaata tttttcgcca
    15181 ttttgtaatt tataactaaa aaatgttgcc atttgattct ctccttactt cattattgca
    15241 ggtagagaag ttatttgaag aatcaattat ttttaaattt atcgtgatta tcagttaaaa
    15301 attttgcgat tatactattt ttctcatatg ggaaattgta ttttttacta agatctgaaa
    15361 taaatttttc tgtttcaaat tggtttttag taattttata aaagaaatcc tcgacagatg
    15421 aatactttga aatatcttta taggctaagt ccatttcgtc taatagctgt tttaaagaat
    15481 caatcattct ttctttttca ccgtatttga aatcaccatt tcttatcttg tcttgaagtt
    15541 tactgttggc tcttttgaaa aatagctctt tagagataga gtgtctttgg ttagcgtact
    15601 cttcaatcag aggattgata ttattagcaa gtatctcatt tagataaatt tcaaaatctt
    15661 ttgagtaata ttttgtaaat atatttttta ttataggatt caataactca tagtctttat
    15721 ctagcgaaaa cgtatcacta tatctttctt gaatgtcttt aaagactttg tgaggtattt
    15781 ctgggaagtc cttaatatat agctcatacc ctatatcgat aaggtatgaa gttatatatt
    15841 cttctaaagt tccccattta atccagtcaa ttgttgaatt agtgatagtt gataataatt
    15901 tcagcttttg tggatttggt aaattggtcc cacgttccca gttggatatg gtcgcacgtg
    15961 gtgaattatc tattttcctt ccaagttctt ccatagtaaa accacatgaa aggcgtaatt
    16021 gtttgattcg ttttcctgct tctgaaggat ttggcttcat cttttttctc ctttttattt
    16081 tt
//
LOCUS       DAYYFD010000065        15909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000065 DAYYFD010000000
VERSION     DAYYFD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.065"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..421
                     /locus_tag="ACHA25_001983"
     CDS             191..421
                     /locus_tag="ACHA25_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8763272.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="ACHA25_001984"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="ACHA25_001984"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8763273.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="ACHA25_001985"
     CDS             1832..2008
                     /locus_tag="ACHA25_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8763274.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="ACHA25_001986"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="ACHA25_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8763275.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="ACHA25_001987"
     CDS             2624..3091
                     /locus_tag="ACHA25_001987"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8763276.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="ACHA25_001988"
     CDS             3176..4060
                     /locus_tag="ACHA25_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8763277.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4410
                     /locus_tag="ACHA25_001989"
     CDS             4174..4410
                     /locus_tag="ACHA25_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8763278.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4498..4578
                     /locus_tag="ACHA25_001990"
     tRNA            4498..4578
                     /locus_tag="ACHA25_001990"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Tyr,seq:gta)
     gene            4585..4656
                     /locus_tag="ACHA25_001991"
     tRNA            4585..4656
                     /locus_tag="ACHA25_001991"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Gln,seq:ttg)
     gene            4781..4951
                     /locus_tag="ACHA25_001992"
     CDS             4781..4951
                     /locus_tag="ACHA25_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763279.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5171..5878
                     /locus_tag="ACHA25_001993"
     CDS             5171..5878
                     /locus_tag="ACHA25_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8763280.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5937..6581)
                     /locus_tag="ACHA25_001994"
     CDS             complement(5937..6581)
                     /locus_tag="ACHA25_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8763281.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6707..6994
                     /locus_tag="ACHA25_001995"
     CDS             6707..6994
                     /locus_tag="ACHA25_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763282.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7102..7422
                     /locus_tag="ACHA25_001996"
     CDS             7102..7422
                     /locus_tag="ACHA25_001996"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8763283.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7503..8816
                     /locus_tag="ACHA25_001997"
     CDS             7503..8816
                     /locus_tag="ACHA25_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8763284.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8813..9532
                     /locus_tag="ACHA25_001998"
     CDS             8813..9532
                     /locus_tag="ACHA25_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8763285.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9660..10211
                     /gene="raiA"
                     /locus_tag="ACHA25_001999"
     CDS             9660..10211
                     /gene="raiA"
                     /locus_tag="ACHA25_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8763286.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10433..12967
                     /gene="secA"
                     /locus_tag="ACHA25_002000"
     CDS             10433..12967
                     /gene="secA"
                     /locus_tag="ACHA25_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8763287.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13093..14194
                     /gene="prfB"
                     /locus_tag="ACHA25_002001"
     CDS             join(13093..13164,13166..14194)
                     /gene="prfB"
                     /locus_tag="ACHA25_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8763288.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14299..14985
                     /gene="ftsE"
                     /locus_tag="ACHA25_002002"
     CDS             14299..14985
                     /gene="ftsE"
                     /locus_tag="ACHA25_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8763289.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14978..15862
                     /gene="ftsX"
                     /locus_tag="ACHA25_002003"
     CDS             14978..15862
                     /gene="ftsX"
                     /locus_tag="ACHA25_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8763290.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
BASE COUNT         5352 a         2690 c         3456 g         4411 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
     4501 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
     4561 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
     4621 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
     4681 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
     4741 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
     4801 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
     4861 gaatattccg aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca
     4921 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
     4981 ttcatcgatt ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc
     5041 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
     5101 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
     5161 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
     5221 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
     5281 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
     5341 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga
     5401 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc
     5461 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
     5521 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
     5581 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
     5641 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
     5701 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
     5761 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
     5821 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
     5881 aacaggtgat ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat
     5941 atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt
     6001 ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca
     6061 cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca
     6121 agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc
     6181 aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct
     6241 cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct
     6301 acattgatca gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt
     6361 ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg
     6421 cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg
     6481 tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca
     6541 ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca
     6601 ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa
     6661 ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat
     6721 gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt
     6781 gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag
     6841 ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg
     6901 gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa
     6961 taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac
     7021 aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg
     7081 ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt
     7141 atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac
     7201 tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg
     7261 gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg
     7321 gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc
     7381 gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat
     7441 cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata
     7501 atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg
     7561 aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga
     7621 ttctttgtca tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg
     7681 gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt
     7741 tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca ggacagctta
     7801 ctgaaaaaca aacggaaatc tcaaagagaa tactttgcca ttcagaaaaa agacatcatc
     7861 ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt ttagtggaaa
     7921 ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat
     7981 tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc catgggaata
     8041 cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag ctataccgtt
     8101 tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct tataacgggg
     8161 atgcaggatt agctcatgct gtgcaaacag cgattaaacc agacggacga ttcatctact
     8221 taagtgccac acctgataaa aagttactaa aggagatcaa gcagacgttt gacatccaca
     8281 aacttgcttt acgctttcat cggcgtttgc ttcctgaacc cgtcttggtt ttcatgaata
     8341 attggcgaca aaaatgcaag aacaagaaga agataaaatc tttggtgcgg atcattcatc
     8401 aattaattaa agaaaaccat atccttttat tttgtccaag tatcacgatg atgcatcgtt
     8461 tgaaagccac acttcaagag gctcttccga gatatgagat cacagatgtt tcttcaagag
     8521 acaatcagcg ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac atactgttaa
     8581 ccacgatgat ccttgaacgt ggagtcacat ttgaaagaat atccgttatc gttttaggag
     8641 cggatcatcc ggtttttact aaatcttctt tggtacaaat cgcaggaaga gccgatcgta
     8701 aagggcctta tacgaacagt caagtatatt ttttctatga agaaaaaact gcagctattc
     8761 gaaaagcttg tcaggaaatc aaagaaatga atgaagaagg gaagaagata ctgtgaggtg
     8821 cagttgctgc agtaaagata tcgtccaaaa tttaacattc agcgagatta ttggtgtttc
     8881 gaaatacaga ttacctcaga cttgttcagt ttgtcgcgca caatttcatt tattggaaac
     8941 aagaggctgt tctggctgtc aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa
     9001 agtaaattct atccccgttt actgcagtga ctgtcaagaa tggaagaaga aatatccgac
     9061 ctatgatttt cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg cctggctcaa
     9121 tcaatacaag tttatgggag atatccgact agcaggcacc tttgcatata aatggacgca
     9181 gataaaaaaa gaatacccga catacatcta ttgtccaata cctttatctg aaaaaaggcg
     9241 aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa ttccttttca
     9301 gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc gaaaggaaag
     9361 attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac aaaataaaaa
     9421 aatattgatt attgatgatg tctatacaac aggacagact cttttccacg cagcagattg
     9481 tttgcagatc gcacagccga aaatcatccg aactatttct ttggcgagat gaattagatg
     9541 tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa tagaattaag
     9601 aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg gtaattttta
     9661 tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg
     9721 ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat gcaacggcgc
     9781 atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg atcccattac
     9841 cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt atcgatttgg
     9901 ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat cgtaaatccc
     9961 gtgaaacggg catgaatact gcaaatgctg ctgctttatt caatgaagag gacgaaaacg
    10021 aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa ccaatggata
    10081 gtgaagaagc agtcttgcaa atgaacatgc tgggacataa cttctttatt tttgaagatg
    10141 cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac ggactgattg
    10201 aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct actgacagct
    10261 gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt
    10321 cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta
    10381 aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa
    10441 tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc
    10501 aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc
    10561 aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc
    10621 ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat atccatacca
    10681 cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg
    10741 tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg
    10801 ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga
    10861 actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga
    10921 aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga
    10981 ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta
    11041 tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat
    11101 ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg
    11161 tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga
    11221 agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa
    11281 tactgcattg acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga
    11341 tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat
    11401 catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt
    11461 agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat
    11521 gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga
    11581 aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg
    11641 tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa
    11701 agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga
    11761 gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa
    11821 tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc
    11881 taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg
    11941 tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg
    12001 aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt cattggaaga
    12061 tgatttgatg aaacgattcg gttcagaacg aatcaaatct ttcttagatc ggatgaagat
    12121 cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca
    12181 aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt
    12241 gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa
    12301 aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc
    12361 gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag
    12421 tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa cagctcagga
    12481 gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact
    12541 aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc
    12601 taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc
    12661 ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga
    12721 tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca
    12781 aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc
    12841 caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag
    12901 aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc
    12961 aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac
    13021 cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa
    13081 gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag
    13141 gtcacaagct tcaggaggtc tctttgactt agaccagctg gaagaagaca ttgcggaagc
    13201 agaagcaaaa atggctgaac ctggtttttg ggatgatagc gaagctgctc aaaaagtgat
    13261 cagtgaaaac aatgcaaata aagaaactta tgatcaattc cattctcttg ctgaagaact
    13321 ggaagaaatc gaattgatgg tggagatgtt gcaggaagaa cctgatgcag ccatgcaaga
    13381 agaagcagaa acacgtatca atgcgctaga tgaaaaaatg aagacatatg aattatctat
    13441 gttgttagat ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc caggggctgg
    13501 cggaacggag tcccaagatt ggggcagtat gcttttacgt atgtatacac gctgggcaga
    13561 gcagcatggg tatcaagtag agacactaga ttatcaagca ggtgatgaag caggaatcaa
    13621 gagtgtgacc ctactgatca aaggacacaa cgcatttggt tatctgaaat cagaaaaagg
    13681 tgttcatcgc cttgtacgta tctcgccttt tgactcagcg aaacgacgtc atacttcttt
    13741 ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga
    13801 tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac
    13861 agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca
    13921 acgttcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca
    13981 attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat
    14041 cggctggggt tcacagatcc gttcttatgt tttccatcca tattctatgg taaaagacca
    14101 tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt
    14161 tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat tgaaataaaa
    14221 ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa
    14281 agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt
    14341 acgaccgcta tccgcaatct ctcaattgtc atcgaacaag gagagtttgt ctatgtggtt
    14401 ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct
    14461 tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt
    14521 ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa
    14581 acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgggatatc
    14641 aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt
    14701 cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatagtgaat
    14761 acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg
    14821 gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca
    14881 cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc
    14941 attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca
    15001 tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag
    15061 tgcggtaacg atcacattga ctttagtcgg tgtattcatg gcagttatca tgaacgcaac
    15121 taaattagct caagatatag aaggaaacgt tgatgtctct gtattcgtag atatcggtac
    15181 aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa
    15241 agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc
    15301 ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac
    15361 tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg
    15421 ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac
    15481 atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac
    15541 gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc
    15601 aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg
    15661 tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa
    15721 tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg tttggaaaat
    15781 caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat
    15841 gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt tggacagttt
    15901 cgttccagc
//
LOCUS       DAYYFD010000066        15750 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000066 DAYYFD010000000
VERSION     DAYYFD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.066"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..627
                     /locus_tag="ACHA25_002004"
     CDS             61..627
                     /locus_tag="ACHA25_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8763291.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            772..859
                     /locus_tag="ACHA25_002005"
     tRNA            772..859
                     /locus_tag="ACHA25_002005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:804..806,aa:Ser,seq:cga)
     gene            1195..2169
                     /gene="bsh"
                     /locus_tag="ACHA25_002006"
     CDS             1195..2169
                     /gene="bsh"
                     /locus_tag="ACHA25_002006"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8763292.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2494..4389
                     /gene="asnB"
                     /locus_tag="ACHA25_002007"
     CDS             2494..4389
                     /gene="asnB"
                     /locus_tag="ACHA25_002007"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8763293.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4584..5030)
                     /locus_tag="ACHA25_002008"
     CDS             complement(4584..5030)
                     /locus_tag="ACHA25_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8763294.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5258..5407
                     /gene="rpmG"
                     /locus_tag="ACHA25_002009"
     CDS             5258..5407
                     /gene="rpmG"
                     /locus_tag="ACHA25_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8763295.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5495..6013
                     /locus_tag="ACHA25_002010"
     CDS             5495..6013
                     /locus_tag="ACHA25_002010"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8763296.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6090..7046)
                     /locus_tag="ACHA25_002011"
     CDS             complement(6090..7046)
                     /locus_tag="ACHA25_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8763297.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7212..8018
                     /locus_tag="ACHA25_002012"
     CDS             7212..8018
                     /locus_tag="ACHA25_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763298.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8015..9004
                     /locus_tag="ACHA25_002013"
     CDS             8015..9004
                     /locus_tag="ACHA25_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8763299.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            9001..10005
                     /locus_tag="ACHA25_002014"
     CDS             9001..10005
                     /locus_tag="ACHA25_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8763300.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10137..10679
                     /locus_tag="ACHA25_002015"
     CDS             10137..10679
                     /locus_tag="ACHA25_002015"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8763301.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            10699..11397
                     /locus_tag="ACHA25_002016"
     CDS             10699..11397
                     /locus_tag="ACHA25_002016"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8763302.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            11418..11630
                     /locus_tag="ACHA25_002017"
     CDS             11418..11630
                     /locus_tag="ACHA25_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8763303.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            11630..12592
                     /locus_tag="ACHA25_002018"
     CDS             11630..12592
                     /locus_tag="ACHA25_002018"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8763304.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            12610..13005
                     /locus_tag="ACHA25_002019"
     CDS             12610..13005
                     /locus_tag="ACHA25_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8763305.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13168..13533
                     /locus_tag="ACHA25_002020"
     CDS             13168..13533
                     /locus_tag="ACHA25_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8763306.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            13530..15341
                     /locus_tag="ACHA25_002021"
     CDS             13530..15341
                     /locus_tag="ACHA25_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8763307.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(15403..15570)
                     /locus_tag="ACHA25_002022"
     CDS             complement(15403..15570)
                     /locus_tag="ACHA25_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8763308.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         4899 a         2646 c         3286 g         4919 t
ORIGIN      
        1 tgctaaaaat agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta
       61 atgagaagaa aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca
      121 aaggaagggt ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca
      181 cagccgattt atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct
      241 atctttgaac aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt
      301 gatctcggtt tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg
      361 tacattgatg aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt
      421 gaaatagtta aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg
      481 catactggta catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg
      541 catccaacag aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca
      601 ttagataatc ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc
      661 gttggctgca aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct
      721 attagaaata ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg
      781 gcagagtggt aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt
      841 tcaaatcctg taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc
      901 aagtaataag aacggccaaa caaaaatagc ttttcctatt ttttcgtttg gttgggctta
      961 ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact
     1021 tttgtcacag ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag
     1081 tactattttt tataaatgct agtaaatcaa tcttctttgg cctttttttt gataaaaaaa
     1141 aatactagat gtaagataaa agtgtaaaaa cgatttgttt taggagggaa aacgatgtgt
     1201 accgcaatca cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg
     1261 tcttataatg aagtagtcac tattactcca agaaattatc gtttcgattt tagaaaggta
     1321 aaaaatttag ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca
     1381 ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc
     1441 aatgctgatt ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt
     1501 ccatggatac tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc
     1561 aatctagtca atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta
     1621 gctgataaag aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat
     1681 aatcctgtcg gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac
     1741 aattatcgct cgctatcaaa tggaacacca gaaaatcatt tttcaaacca gatcagcttg
     1801 aatgtttata gtcgtgggat gggaggattg ggcttacctg gagatctctc ttctgtttct
     1861 cgatttgtaa aagccacatt tactaaaatg aatgctgcat caggtgattc agaatctgaa
     1921 agtatcagtc aattttttca tatactaggc tcagtcgagc aacaaaaagg agtatgcgat
     1981 actggagaag gaaaatacga atatacgatt tattcttctt gctgtaacgt tgataaaggg
     2041 atttattatt atcgtacgta tgaagacagt caaattactg ctgtaaatat aaataaagaa
     2101 gacttggatc gtcatgaatt ggtcagttat ccagtcataa aagagcaaca gataaactat
     2161 gtcaattaat taatgaatag atccgtttgg tatcatattt tctgatacag tcataattag
     2221 acacgatctt taggtcgtgt ctttttttgc tgtttttttt ggaaatagca tccactaata
     2281 tttttttaac acgaagagaa taaggtagcc tcaaaaaaga aaacatggta aattagatat
     2341 tgggacttta ttttctgtaa aaacagaaac tttcatcttt tgttttcacg tgacgaaatt
     2401 gtaagagaga aaatattcgt ccgctattta ttctatgata agatgcatat gaatgaaata
     2461 aacagtaatt ttatattagg atgtgaaagt taaatgtgcg gtattgtagg ttttgttaac
     2521 gacaaggaca acaaaaaaac aattattaat acaatgatgg atcgaatcgt tcaccgtgga
     2581 cctaatagtt caggagaata tatcgataaa catgttgctt taggattcag aagattaagt
     2641 attattgact tagagggcgg tactcagcct atttacaatg aagatcggac aaagatcatt
     2701 atttttaatg gcgaaatcta taattaccag cctttgagag aagaattgat tgcagctggc
     2761 cacgtttttc aaacacatgc agatacagaa gtccttttgc atggttacga agaatgggga
     2821 acagaattat tgcaaaagat ccgcggaatg tttgcttttg ctatttggga taatgaaaag
     2881 aatgagttat tcggtgcaag agaccacttt gggatcaaac catattatta tgcagaaatg
     2941 aacggaacat ttatgtttgg ttcagaaatc aaaagttttc tgcctcatcc tgattttaac
     3001 aaagaattga acaaagaagc gttgaaacct tatatgactt tccaatattc tccattaaac
     3061 ggcgaaacat tcttcaaaga tgtctatcgt ttgcctgaag gccattatta tacgtataaa
     3121 gatggaaaac tagacattca gcaatattgg gatgcagatt ttgaaacaaa agaaacgcat
     3181 tcccgacaag aatggatcga aaaaatcgac gaaaccgttc aagcttctat tgaagcacat
     3241 acggtcagtg atgtagaagt tggttctttc ttatccagcg gggttgattc cagttatgta
     3301 acctctgtat tgaaaccaga tcattctttc tcaatcgggt ttgatgataa aacctataat
     3361 gaagcgattg aagccagaaa actaaccgaa ctattggact tagataatac agcagcagtg
     3421 atcgacggag acatgtcgtt caaagcattc cctttgatcc agtatcactt agatgaacct
     3481 gattcaaatc cttcttgcgt acctttgtac tttttagcaa atcttgcttc acaaagtgtg
     3541 cgggtagtcc aatctggaga aggggcggat gaactgtttg cgggatacca aacgtatgga
     3601 ttccatacga attcaaaatt catccgtgtg attgctcaag gattgaaaaa attaccaaaa
     3661 ggaacacgct ataatttagg acgtaaaatc ggaaaaatga aaaatttcca tggacggatc
     3721 catttatatg aatcacttgc tcctgcaaaa gaatatttta tcggtcatgc tcgagtgttc
     3781 gaagaatcag aagcatcaga agtgttaaca ccaaaatatc aaacagcacc atctgtggat
     3841 gaaatcatga ctgttcatta cgagaaaaca gaaggaatca aagatgaagt caataagatg
     3901 caatatgtcg atcttcatca atggatgcca aaagatatct tgttaaaggc agataaactt
     3961 tctatggcaa gttctttgga agtccgagta cctttattgg atatcgaagt catgaaattg
     4021 gctcaacaaa taccaagtaa gtttttattg aatcaaaaca acacaaaaga tattttcaga
     4081 caagctgcga acaaacatct tccggaagaa tggtcgaatc gtgtgaaatt aggattccct
     4141 gtgccaatca aagcttggtt gaaagaagaa cacggctacg aacaagtcaa agcattattc
     4201 gaagcagatt ttgcaaaaga attttttgat caagaaaaaa tcatgaagtt gctagatgac
     4261 catcacgaag gacgtaaaga agagcaaaga aaaatctgga caatcttcag tttcttgaca
     4321 tggtataaag tctactttgt agatgaaacg attccgcaag cagaagcaat cgactatgtg
     4381 actgtttgat gaaaatcaaa tatcaataaa aaagaggttt tctcctaccg tttaacactg
     4441 agtgataaga aacaatttca aaaaacagct cttcccgttt tatagatatt atgacttatg
     4501 gttcggagtt ggcagaggaa caccattgtt tcgaacataa gggggttgtg acacgatttt
     4561 cgtcacaacc cctttacata tgttcaataa tcgatttctt tttcttttgt cccatctttt
     4621 ttataaatga acaaagttga ttctttgttt tgtgctactt gctttccttt ttcgattgct
     4681 tctttttttg tatcaaaatg gttgcttgcc ttttttgccc ctttagattt cacgatccat
     4741 tgttcttcct catattctac gatcacatct gaatcaagca gttttccagc acgaggattc
     4801 gtatcgtgtt catccgtctt gcttggattt ttttcttttt cgaaagttcg tttttctgat
     4861 tcactagcat cttcatacca tttttttgct tgatcaatgg caattgggat cgcacggtta
     4921 tcagggtacc cttcatctaa taatgcattt gcgatgtcga ttgctttttt tcttgtcaat
     4981 ttatctaagt ttttcattga tgcaggaaag tccttcatgt tccatggcat attagtatcc
     5041 ccctttatta aaaaacataa tctatataaa caagtgtaag ggtatttata caaaaagaaa
     5101 aagattttgt ttgagaagta aaaccgttgt atttatcgtc aagtagtttt acttaacaaa
     5161 atcgtgaaaa acactagcaa tctagaaaaa atcatggtaa gataatcaag tctgagagtc
     5221 atcttagagg attcttgtaa aaatgggagg gaaaaacatg cgcgtaaaca tcactttaga
     5281 gtgtacttct tgtaaagaac gtaactatct aacaagcaaa aacaaacgta acaatcctga
     5341 tcgtttggaa aaacaaaaat attgcccacg cgaacgcaaa gttactttgc accgtgaaac
     5401 aaaataatgt ttttaaagtt ctcaattcct tgctggtcaa agaattgaga ctttttttat
     5461 ctcaaaacat ctaaaatcat aaggagcgga taatatgttt atttccatgt gggcacaaga
     5521 taaaaatggt ctgatcggca aaaatggttt gttaccatgg cgattgccaa atgatatgcg
     5581 tttctttcgc gaacatacaa tggatcgaat tttagtgatg ggaagaaaga cctatgaagg
     5641 aatgggggat ctttcacttc cttatcgcca tatcatcgtg ttgacaacgc aaccaaattt
     5701 tcgcacgaaa gaaaatgcgg aagtaatgca ttcgatcgat gaacttttgt cgtatagtca
     5761 aacgatctca gaagatatct atgtttcggg tggtagtcgg atttttcaag aacttcttcc
     5821 ttacactggt cggatctggc gaacattgat cgatagcacg tttgaaggcg atacgtatat
     5881 tgggaatatt gatttttcag acttcgccct tatagaggag tatgagggga taacggatca
     5941 agaaaatcta tatgcccatc gttttcaaaa atgggaaaga gtaaaaaata gggttgcttc
     6001 aaaagaagaa taaaatctat gcgtctagtt tgttaataag aaaaaaagca tctcacgact
     6061 aatcgtgaag attctttttt tagttaaaat caatttagtt tttctttgat atcattgacc
     6121 atttcctgac tggcaatagg tccgttatat agccaactag attcaggtcc gaattgcagt
     6181 acatgattgt ctgtcactgc tttaagattc ttccagcttg gttcattgaa catggcagca
     6241 tctttgtcac tgttcacaag gatgatgtag tcagcatcta attcagctag cttttcagag
     6301 gaaacagcag accagtcaga agtggctttt ttggagacct cttccactag atcaggaaca
     6361 ccaaattcca gatcgtcgta tacgattctt ccacttgacc gatttttgct gaccataaag
     6421 gctgagttgt tggtcaccca caacactgca gctgtcttgt tctcgatttt atctgctagt
     6481 tcttctcgag ttttcttgat ggatttttga taatcggaaa tgattttttc tgcctctttt
     6541 tcttttgcca aagctttgcc gacatctttt agttgttctt cccatgtgac atctgttccg
     6601 ttttttacaa cataagttgg cgcgattttg ctatattgtt cgtatttccc gccttcgaca
     6661 gtagcggaag aagaaatcaa cagcaaatca ggctcaaagc ttaacacttt ctcataagga
     6721 aggtcataag aaatcgttgg gacatccttt aaatcatctt gcagataatc ttgaatggaa
     6781 ccactgccaa cagtccactg ggcgataggt ttttccccta gtgcaatcag atagtcttct
     6841 aagtaactgc cgatgatccg tttgggcgcg tttggaatct ctactttatg tccaagtgta
     6901 tctgtgactg tatgagtact attggatgaa gtcgtcgaat tggcagttgt agctgtttct
     6961 ttatctgaag agggggagca agcgctcagt actccagcca gcagcaatat gctagataat
     7021 gtgaatactt ttgtttttaa tttcatttct ttcctcctaa cataattgag aatctttctc
     7081 attggttatt attcctgata tttcgaacgc tgtcaaccgc ttcatttggc tatagatatg
     7141 aaagtagcgc ttgacgaaat gttgaaacag cgtcataatt gagaatgatt ttcagcgaaa
     7201 ggatgggtga aatgacgaga ttaagagcag ctgacattgc agccggctac gaaaacaaaa
     7261 aaatcattga aaacatgtct gttacgattc ctgaagggaa aatcactagt ttgattgggc
     7321 caaatggaag tggaaaatct actttattga aaagttttac ccgtatacta cctgtgaaat
     7381 caggaaagat cattgtcaat aactctgata tctctacata cagttcaaaa gaacttgcaa
     7441 aaaaaattac attattagca caaactagtg aacaccctac aggtatgaca gtagaagaag
     7501 tagtatctta cggacgatac ccttatcaaa aactgttcag tggcatggac acgcaagatc
     7561 atgaagcaat cgagtgggcg ttggatgcta caggtctagc tggatttcgt atgcgggata
     7621 tcgcaacgct gtctggtgga caaacccagc gtgtctggat cgctatggcg cttgctcagg
     7681 aaacagatat cttgatcttg gatgaaccga caacattttt agatccggca caccaattag
     7741 agatcctaca gttattgact gaaatcaatc aaatgaaaca aacaacgatc ttgatgtcga
     7801 ttcatgacat taatcatgct tcacgttttt cagattattt attagcaatg aaaaacggta
     7861 agttgatcac agaaggtaca cctgaagaag tgatcacaaa agagtgtatg aaaaaaatat
     7921 ttgagattga tgcaacgatt gtcacgcttc caagaacaaa taaaccagtt tttctaacct
     7981 atgatttgtt gccaaaagag caggaggatc aattatgagg actatgaaga aaaatagact
     8041 cctgcttttg gtctgttttg ttcttttgat tatttttttt gttgctcttt caaatggcgc
     8101 agcgactgtg ggaaaagcag atttttggca ggcgttgttt gattttgata gatcaaaaca
     8161 aagtcaccaa atcatacgaa atattcgttt gcctcgtgtg gcgggagcat ttcttgtagg
     8221 gagttgtttt gcattgagcg gtgcattgat gcaaggagtg acaagaaatc ctttagcaga
     8281 ttcaggctta ctaggaatca acgcgggtgc ttcgttggca atggccctta gttttgcttt
     8341 ttggcctaaa gcttctgatt tttctgtgtt tctaatgtca ttggcaggtt cttttgctgt
     8401 aactttgctc gtatttggtt ttttgcagtt ttcgaaaatg ggcatgaatc ctttacagct
     8461 gattttagct ggcgtagcta ttagttcttt ttttactgct atcagccaag cacttaccca
     8521 attctttaat ctgcagcagg atcttgcttt ttggtttgta ggcggagctg ccaatatcac
     8581 atggaaacaa ttagcagtat tgtttccttt atatatactt gctgttttgg tttctttttc
     8641 attaggaaaa gcaatatctt tgattcattt gagtgaaaca catgcattag ctcttggaag
     8701 tcatcctaaa cgaacgcggt tgattgtttt ctgtctagtt gcagtgttag cagccattgc
     8761 cgtttcgtta gtcggacctg tttcatttat tgggctgttg gttccgccag ttgttcgcgg
     8821 aatggttgga acggactatc gttatgttct acctgtctct tttttagttg gcggtggatt
     8881 ggttatgttg gcggatttta cagcgaggat gatcaatccc ccttttgaaa caccttttgg
     8941 attagtcatt gcattgattg gcgttccgtt tttactgata caagtaagga gggaacaacg
     9001 gtgaaaaaaa tatattggac gttctatttc ttgctttttg tacttgtcat cctgtctata
     9061 ggtagtttaa tgattggtac gccttttatt tacctggatc agctttttgc cacgttcata
     9121 gggaaagaat catcttcttt ggcgctgatt gttagagaat ttcgagttcc tcgattactg
     9181 atcagtttat tagcaggagc ttgtcttggc gtttctggtt atatcttgca aggagtaaca
     9241 agaaatgaat tggcagattc tagtatattg ggcattaatg ccggagcagg atttttagtg
     9301 atgctttatt taggtttctt ttcgcactat tcgctgccgt ttctattgct agttgtagcc
     9361 tttctaggtg gtgttttagc tgcttgctgt gtctatcttg cggcctatga tcgaaatggc
     9421 tttttgggta tgaatcgaat cttattgtcc ggtattgccg ttaatgccgg cctttctgca
     9481 ttaacattgc tatgcacgat ccaattatct aaggaaaatt atggatttgt gaatgcatgg
     9541 ttagcgggat caatctgggg tgcgagctgg ccttatgttt gggcattgct tccatgggcg
     9601 gttatcttaa ttccgctagg aggactttat gcacagagga ttggattgct atcatttggt
     9661 caagaacgcg cgcaaggatt aggccttaac gtggctaaaa gtcaaaaaat ccttttgttg
     9721 ttagctgttg ctttggcaag cggtagtgta gcggtgacag gtagtttatc ttttgtagga
     9781 ctactagctc ctcatgctgc gaagctagtc gtacgtaaag aaaataactg gacctttgtt
     9841 ctgagcggtt tgttcggttc catttttctg cttagtgcag atatggttgc tagagtgatt
     9901 ctaccaagtg gggaagtacc tacaggaatt ttgattgccc tttttggtgc gccatatttt
     9961 ctgtatgcac tttttatcag acaaaaacac gcgctgaaca gttgaaattt attaaaaaag
    10021 gaaggccaat catttctagg tcttcttttt attctattta tgcatttatt tgaaagttcg
    10081 attgctattt ttcttttttt cctataccat tttatgagag aaggaagggt gagtttttgg
    10141 accaattgaa agtaagatta cgccggaaag gaatcgaaaa gctgaaaaat ctggccaaaa
    10201 gtggcaagaa aaaggaaaaa gaacagcgaa tctataaaca gctattcgcc agcgagtact
    10261 ggaagtcagc aaaagtgata gggacgactt tatcgaatga atttgaactg gatacacagc
    10321 cggttatcca acgagcagaa aaagaacaaa agaaaatagt tgtacctaaa actttgccga
    10381 aacgtcagat ggcgttttat gaaatggatg aacagtccgt tcttgtacgt tcgagttttg
    10441 gtgttcttga gccacaatca gagaagctct atttgccgga gatgatcgat ctcctcattg
    10501 taccaggtat tatctacaat agagccggtt atcgaatcgg ttttggcggt ggttattacg
    10561 accggtattt aaataatttt cataaaaaaa catgtagcct tgtttttcgt gaacaattga
    10621 atgaacaatg gcaaccggag agctttgatc agaagatcca acaattgttt attgactaaa
    10681 atgaaatggg aggttttcat gaattatcaa cagcaaataa aattgcgtgc atggttaaga
    10741 cgaccgttat ggacctatgc ttttttaggg atacagacag cggtcttcct catgatggaa
    10801 ttatttccgc gacttgagat cccttattat gccggaatgt atggtcctta tcttgttcac
    10861 ttcaatgaat ggtggcgttt agtgacacca atcttcatcc attttggact gatgcatttc
    10921 gtaatgaact cactgatact gtattttatg gggcagcaaa tcgaggcaat ctatggccat
    10981 tggcgttttt tcttgattta tatgttcagc gggataatgg gaaatacagc aagctttgct
    11041 tttaacgaag cgaatgttct gtctggtggt gcgagtactt cgatattcgg tttgttcggt
    11101 gcgttgttta tcttagggtt tcattttaaa cataacccag cgatccagca gctagtcaga
    11161 cattttcttt tatttattgt gttgacattt gtttttggtc ttctggatac ctctgtagat
    11221 gtttggggac atgtcggtgg gctaattggc ggattagtgt tagggaatgt tttgggtcta
    11281 ccgaaacaac agacaagcta ttcgatacac caaaggattc tttctacact agtttttgta
    11341 tttctttttg taatatgtat tttactgggt ttaaaaaaat atggtttact tgtataatat
    11401 gaaggaagtg cttaaagatg gagagcttat atgatgtaca gcagctgttc aaaaaatttg
    11461 gcatctttat ctatgtgggt gcacgcatct atgacattga attaatgact atcgaattgc
    11521 gtaatctgta tgatagccgt ttgattgatc atgatacata catgacagcg tggcgcatat
    11581 taaaaagaga gcaccgaatt gaagaaagcc gaaagaaagg aaatttgtaa tggacaagaa
    11641 gattattggg attgatttag gtggaacaac agcaaaattt gcaattttaa caccagaagg
    11701 agagattcaa caaaaatgga gcatcgatac caatattttg gatgatggta agcatatcgt
    11761 tcctgaaatc attgaatcaa tcaaccatcg tttgaattta tataatatga aagccgaaga
    11821 ctttatcgga atcggcatgg gtaccccagg aagcgtggac agcgaagctg gaacggttat
    11881 tggtgcgtat aacttaaact ggacagaagt tcaatttgtg aaaaaactga tcgaggcagg
    11941 aacaggaatc aaattcgcta tcgataatga tgcgaatgtt gctgctttag gtgaacgatg
    12001 gaaaggtgct ggagaaaacg atccagacgt tgtattcgtt actctaggaa caggtgttgg
    12061 cggtggtatc gttgctggcg gaaatttgat tcacggcgtg agcggagcag gcggagagat
    12121 cggccatatc actgtggatc ctgaagggtt cgaatgtact tgcggtaaac gcggatgttt
    12181 agaaactgtg tctagcgcta ctggtgtggt acgtctaggt cgttacttgg cagaagaata
    12241 cgcaggagat tcaaaactga aagcaatgtt agacaacggt gaagaagtaa caagtaaaga
    12301 tatctttgaa atggctcaag aagacgatcc gtttgcattg atggttgttg atcgcgtatg
    12361 cttctactta ggtttagctt gcgccaactt ggggaataca ttgaatccgt caagtattgt
    12421 gttaggtgga ggagtatcgg cagcaggaga atttttacgt agccgtgtag agaaatattt
    12481 taaagaattt actttcccgc aagtaagaga atcaacaaaa atcaaattag cagagcttgg
    12541 taatgaagct ggtgtcattg gtgctgcctc attagccttg aaatttgctt aaaaaaacgg
    12601 aggcattaca tggttttatg ggtaattaat atcattttgc tattgattgt gcttgcaatc
    12661 atcttttggg aagtctatct acgagtaatg gccaagcgtt cagcaacgac tttgacagaa
    12721 gaagaatttc gagaaggaat gagaaaagca caagtaatcg atgttcgcga aaaagatgtg
    12781 tttgatgctg ggcatatttt aggtgcaaga aacatccctt acactgtttt gaaagattca
    12841 ttaggatcta ttcgaaaaga ccagcctgtc tatatttacg atcaaaagaa aagcttgagc
    12901 atccgtacag caaataaatt acgtaaagca ggttatcaag agatttattt acttaagggt
    12961 ggatatgacg gatggtctgg aaaaatcaaa agtaaaaaag tataaaacag atgattgtat
    13021 gacagcttta aaaaccgaat caatcattag attttgacca agatcacatg aacggcgcaa
    13081 tggatagttg tgccgttcat tttttttatt ttgtgctatt atctacatat ataagctaag
    13141 cgaaaaggca gaaagaagga aacaactatg atcaaattaa aacgtgttta ccttgatgct
    13201 gaaaaagagg atggctatag aatactcgtg gataggttct ggccacgagg aatttcaaaa
    13261 gaaaaagcgc agttagatct gtggttgaaa gaaattgcac ctagtaatga agcacgcaaa
    13321 acttttgacc atgtaccaga acgatttgct tcatttgcaa agaaatacca gcaagaattg
    13381 attttcgaag aagaaaagaa gcaagctatt tttaaactca gtcaacttat gaaagaacat
    13441 ccagtcatta cttttgttta tggtgcaaaa aatgaacatc agaaccatgc tgttttattg
    13501 aaaaaattta tcgaaatgga ggcatgcaaa tgaaaatagg gatcattggt gccggaccta
    13561 gaggattaag tatagcagaa aggattctcc ataataatca ggacagacat tctcttgaac
    13621 tttttctttt cgatcccaat ggtcctggag gtcgagtttg gcggttgaat cagccaccgg
    13681 agttattaat gaattctgtt tctcaacaag tgacgctttt tacagatgaa acattgtcca
    13741 gtggaggatt ggtaacacag ggacccaact tgtttcaatg gagtaaagga cctgctacag
    13801 attatatcga acagacggta accaaaaata agcaactaat tatagaagaa atcaatcgtc
    13861 tggaaaaaga tcagcaatct actcgatgtt tatacggact ctatcaacgc tggtttttct
    13921 caaagttgca agaagaattt ccagcttcta ttcagtttgt ttattcttta gtaaaaaaag
    13981 tcagcaaaag aaaagatggt tatcttttac agacagatca gcaaagctat tctatcgatc
    14041 agcttgtttt agcaagcggt cactgggaaa atgatgagac agaagaagaa agagtgtggg
    14101 tagactacgc aaagaaaaat gatttactgt atcagcttcc ttctaatcct gcagatgtcg
    14161 atctttcgaa gataccgcct aaagaaaatg tctttttaag aggtctaggc ttaagttttt
    14221 ttgattatgt cggattgttt acagtatcaa gagaagggcg cttcgagaac gaaaaaggaa
    14281 aacttgtcta tcatccatca ggcaaggaac cgattgtata cagtggatca agaagggggc
    14341 tcccttatta ccctcgagga agaaaccaaa agcaaggcgg atcaatggca tggccacggt
    14401 tactaacaaa agaaaacctg gagcaatggc atcagtcggg atttttgact ggcgaaacat
    14461 tttttgacta tctgaaaaaa gacgttgagt tgttttatta caagaaaatc atcgaagaat
    14521 atcatttgcc gattccaaag aaaaattttg agcatgattt tctctccctc tcttcagagg
    14581 agtgtctcgg aaagcatcca gcactaatcc cttatagatg ggattggtcg tatttagaaa
    14641 ttcctcttac ctatcaggat gagtcctttg cagaagctag ccgagcattt attgaagggc
    14701 agataaaaga agcggaaaaa ggcaattgca caggagcatt tgcttcaaca tttgatgcat
    14761 taaaagactg gcgtgatcca atccggcagg ctattgaatg gcacgttttt tctgcaaagg
    14821 aatacaaaga aatactatgg ggatggttta ctcatctaaa tagcttttta acgatcggtc
    14881 cgccagtcat tcggacgaaa gaattagcag cactgataga tgcgggtatt tttcatttgt
    14941 tcgaaccgcc atttgatatt cagcaaaagc acaaccgcta tgttatttct ctacaagata
    15001 aagaaatcag cagtcgctat ttgattgaag ctcgattgcc acaaacaagc ttttctcgtt
    15061 caagtaatcc tgtcataaaa aatttgaagg aaaacaaact tgttcgccct ttttcatttt
    15121 cagataatac aggaagttat cattcagaag ctatagatgt tgagcgaggt acgaatcgcg
    15181 tgattgatgc ttcaggggaa atccagtctg acttttattg ctatggaata cctgttgaag
    15241 gtgtggattg gctaactgca gcagtagcta gaccttatac agatccatgg aatctgagac
    15301 aggcagataa aatagcacaa cttatattga ctgacaaata aaaatacgca tgatgactta
    15361 tctgttcttt cacaagtctt catgcgtgtt tttaaataat tattaacgag aaacgcgttt
    15421 gcgcattgct tttttacggt tttcttcgat cattgcttct ttttcgtcaa ttggatcgat
    15481 cactgttttt ttcacagaag cgaccaaacc agcaacggca gcggctgttg ccagacttcc
    15541 tactaatatt ccagatacaa attttttcat tgaatccacc tcatttatta tttgttatat
    15601 ctattatgga acaaaagaga aaaaaaggct agttttacgt ttgtttgtaa acctttgcaa
    15661 aaaatccttg aaaatgttta ctaaatgttt tattataaat ttaccatatg aatttacgaa
    15721 attttacttt gtttgttttc accataaaag
//
LOCUS       DAYYFD010000067        15660 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000067 DAYYFD010000000
VERSION     DAYYFD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.067"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHA25_002023"
     CDS             complement(<1..132)
                     /locus_tag="ACHA25_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8763309.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            541..2913
                     /locus_tag="ACHA25_002024"
     CDS             541..2913
                     /locus_tag="ACHA25_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8763310.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2974..4434
                     /locus_tag="ACHA25_002025"
     CDS             2974..4434
                     /locus_tag="ACHA25_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8763311.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            4445..6649
                     /gene="topB"
                     /locus_tag="ACHA25_002026"
     CDS             4445..6649
                     /gene="topB"
                     /locus_tag="ACHA25_002026"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8763312.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6665..6814
                     /locus_tag="ACHA25_002027"
     CDS             6665..6814
                     /locus_tag="ACHA25_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763313.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6889..7482
                     /locus_tag="ACHA25_002028"
     CDS             6889..7482
                     /locus_tag="ACHA25_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8763314.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            7495..8394
                     /locus_tag="ACHA25_002029"
     CDS             7495..8394
                     /locus_tag="ACHA25_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8763315.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            8397..8894
                     /gene="ssb"
                     /locus_tag="ACHA25_002030"
     CDS             8397..8894
                     /gene="ssb"
                     /locus_tag="ACHA25_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8763316.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            9260..9520
                     /locus_tag="ACHA25_002031"
     CDS             9260..9520
                     /locus_tag="ACHA25_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763317.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9965..10171
                     /locus_tag="ACHA25_002032"
     CDS             9965..10171
                     /locus_tag="ACHA25_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8763318.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            10192..10422
                     /locus_tag="ACHA25_002033"
     CDS             10192..10422
                     /locus_tag="ACHA25_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763319.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            10438..10875
                     /locus_tag="ACHA25_002034"
     CDS             10438..10875
                     /locus_tag="ACHA25_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763320.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10868..11575
                     /locus_tag="ACHA25_002035"
     CDS             10868..11575
                     /locus_tag="ACHA25_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763321.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11739..11939
                     /locus_tag="ACHA25_002036"
     CDS             11739..11939
                     /locus_tag="ACHA25_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763322.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11955..12134
                     /locus_tag="ACHA25_002037"
     CDS             11955..12134
                     /locus_tag="ACHA25_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763323.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            12459..12722
                     /locus_tag="ACHA25_002038"
     CDS             12459..12722
                     /locus_tag="ACHA25_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8763324.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12716..13066
                     /locus_tag="ACHA25_002039"
     CDS             12716..13066
                     /locus_tag="ACHA25_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8763325.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(13090..13704)
                     /locus_tag="ACHA25_002040"
     CDS             complement(13090..13704)
                     /locus_tag="ACHA25_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8763326.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            14018..14440
                     /locus_tag="ACHA25_002041"
     CDS             14018..14440
                     /locus_tag="ACHA25_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763327.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            14450..14653
                     /locus_tag="ACHA25_002042"
     CDS             14450..14653
                     /locus_tag="ACHA25_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763328.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14864..15448
                     /locus_tag="ACHA25_002043"
     CDS             14864..15448
                     /locus_tag="ACHA25_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8763329.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5757 a         2546 c         3158 g         4199 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaagaac
      541 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
      601 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaaaaatg
      661 acgaacggtc aaccaatgaa cgacaagagg aatggccgtt catggtttaa ccagcaagaa
      721 cgaatcgaaa ataaagtcca aagtctgaga gaagagatca agcaacaaga agaatacgtg
      781 gaaaagcttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      841 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      901 gaatcggctt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      961 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
     1021 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
     1081 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
     1141 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctagacca
     1201 actgattcta tgaaggagaa agaaaacgat catttctatc gagtagaatt caacgaaaat
     1261 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
     1321 caactcaaaa cattggataa ccaatacgcc gcagaagcag gttattataa attctatttt
     1381 gaggaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
     1441 aacagtaatc aaccaatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
     1501 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1561 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1621 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaagac
     1681 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1741 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1801 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1861 aaagggtttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1921 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta tacgattgaa
     1981 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
     2041 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
     2101 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
     2161 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
     2221 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     2281 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     2341 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     2401 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     2461 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     2521 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2581 caggagcata aaaatacttt agaaacgcca gagaataaga atattgtgcc cgttggttca
     2641 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2701 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtaatttt aaaaggaaaa
     2761 agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
     2821 ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga aatctattat
     2881 gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact atctgaaaaa
     2941 cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat gagtcttatt
     3001 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     3061 aataaaggac gagattatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     3121 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     3181 tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc cactgatttt
     3241 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga aacagaaaag
     3301 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     3361 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     3421 gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     3481 gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc gagttatcaa
     3541 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3601 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gataaaattt
     3661 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3721 acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3781 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3841 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agtcgatcca
     3901 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3961 gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc agaagctatt
     4021 atggccatac acaagacaga aaacaatctg caattaacga accaactggt ttccgctcac
     4081 aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga agccattaat
     4141 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg cgtggatggt
     4201 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca gattcttttc
     4261 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
     4321 gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca agaaatcaga
     4381 aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga gtgaaatcgt
     4441 catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt atgccgatag
     4501 cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat tgtttcctgg
     4561 tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc ccgatatgta
     4621 cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta cacattatca
     4681 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     4741 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tcgcttggac
     4801 gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt ggatcaactc
     4861 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa aagagaccta
     4921 tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg ggatgaacgg
     4981 tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct tttccttagg
     5041 cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga ttagaaattt
     5101 caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg gcgcatttga
     5161 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     5221 agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg aaaccgagaa
     5281 aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta aaatgaatca
     5341 gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt atgaagggaa
     5401 atatctaagc tatccgcgaa cggatacccc ttacattacg gaaggggaat atgcgtatct
     5461 gttggatcac ttagacgaat acaagcattt tttgagggct gaagaaattt cgactcctat
     5521 tcatacaccg aatagtaggt acgtgaacaa caagaaagtc caggaacatt acgcaatcat
     5581 tcctacaaaa acagttatga cagaagcgac gtatgaaagt ctgacacctt tacaacaagc
     5641 gatttacgag caagttttaa aaacgacagt ggcgatgttt gcggaaaaat atacctatga
     5701 agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg gaaaagtacc
     5761 gatagatcta ggttggaaga agttatttgg caaagagagc gacggaaaag agaaggaaga
     5821 agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc tacaagtcct
     5881 tgaaaaagaa accaagccac cacaacctta taccgaaggt acgttgatca ctgcgatgaa
     5941 aacagctggg aaaaccgtgg atagtgaaga agcgcaatcc atattgaaag aagtggaagg
     6001 aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa aggaatatat
     6061 caaggtagag aaaaataagc ttgtggtgac gaataaaggg gttttgcttt gccaagctgt
     6121 ggaaaaagag ccgctgctta cgagtgccga aatgaccgcc aaatgggaaa gctacttgtt
     6181 aaagattgga gaacgaaaag gaacacaaac cacttttcta gccaatatcc aaaagtttgt
     6241 ctctcactta ttggaagtcg tcccaggaca aatccaatcc attgattttg gatcgacgtt
     6301 acaagaagtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag gaatttgtcc
     6361 aaaatgccag gaacaagaag tcttactcta tcaaaaggtg gcagcttgta cgtctgaagc
     6421 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcga cacagctgaa
     6481 ggagattatt caaaatagcc gaacaagcca accagtgaaa gggttaaaag gtcagaaggg
     6541 ttcgtttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg aattttctga
     6601 aaagaaaaaa acgaacttca agaaaagaac acgacgcacg acaaaataat ggagggttaa
     6661 tcttatgcgt aatacaacac acaaacaaca agaaatcgaa aatcaaaaag ccaaaattcg
     6721 ttcagaacaa gagaaattac gtgccagtaa aagaagactt cagcaattaa agaaccagca
     6781 acggattcaa aaagaaatcg aattagatcg atagacatta accttgttac aaatacacta
     6841 tcatgatata tttaatgtag tgtatttgta acaaaagggg ggaagtaaat gcaagagaaa
     6901 ctgaaagaat tgttggctat tcgcgatgga aatttatcta tgaaggaagc cagaaaatta
     6961 ggaatcgcag caaccactgt acaaagattg gtgaatcaag gaaaattgat caaagttgat
     7021 cgtggttatt atgtagagga tggtcatgga atagatgatt tattttggct tcagcaacgt
     7081 ttttccaggg gaatcttctc tcatgaaacg actttagata tttaccagct ttctacgaat
     7141 atgccgaaat tcattcactt aacttttccc catggatata acgttgatcg ttcaattaca
     7201 aaagagcagc agttacaatt tcatttcgtg aagaaagaag tttatgaact aggaaaagta
     7261 gagggaactt cctttcaagg aaatcctatt actatgtatg ataaagagcg tacactttgt
     7321 gatatttgga atccgagata tgaaattgaa tatgaaatga aattagaagc attaaaagag
     7381 tacatggaag acccattgag agatccaagt aaattaagag attacatgac aaaactacat
     7441 gtagaaaaag aaatgaaata ccacatgcaa agcttatatt gatggaggaa aaagatgaca
     7501 ccgacacaat ttgcagcaaa aacaagaaat ttagcaaaga ggacaggatt aaatgcacag
     7561 ttagtacaac ggcattttat gatggatcgt ttaattgaac aaatttctga atctaagtat
     7621 aaaaatgatt tcattttgaa aggtggcttt ttattaggat caaaatatgg aatcgataga
     7681 aggacgacaa ttgatatcga cacgacattt agaaatagca aattaactga agaaaaattg
     7741 aagagtattt ttaatgattt aactaggaaa cccacgaaag aaggtattgt tttctctatt
     7801 caaggaatga aggaaacaag agaagccgat ttttatcctg gatttcaaat taaattggtg
     7861 gctaatttag agaaagtacg tgtgccattt aaaatggacg tgacaacagg agattctatt
     7921 tatcctgaag tagatacaca ctatcaccaa ttaatgtttg aagataaaaa ggtggagatt
     7981 ccagcttatc caacagcaca aattattgca gaaaagctaa gtgctacgtt cagctttggc
     8041 acagataatt cgagagcaaa agattactac gacttattta ccattccgaa actagaaaat
     8101 atcgatacga aagagttata caaaagtgtt cgaaatacat ttacaaaacg tggggataca
     8161 aaaccgttgt ccttttatta tgaaagagaa atggataaga tcataggaaa tgactatttg
     8221 gaaaagcagt gggaaaaata tcgctcagtt aacacgtttg cgaattccat ttcatttaca
     8281 gatacagtca acgaaattga tcacctaatg aaaagtgtga tcaaaattga aaatcaagaa
     8341 agaaaacggc agtttaaaag atcacaacaa atgaatcaag aaatggagag ataaagatga
     8401 tcaataatgt aactttagta ggacgcttaa ccaaagatcc agatttacgt tataccgcga
     8461 gtggtactgc agtagcgact tttaccctag ctgtcaaccg aaatttcacg aatcaaaacg
     8521 gcaatcgaga agctgatttt attaactgtg tgatttggcg caaaccagca gaaacaatgg
     8581 tgacattagc taaaaaaggt agcttgattg gtgtagttgg gcggattcaa acgcggactt
     8641 atgacaacca gcaaggacaa cgtgtgtatg tgacagaagt tgtggccgat aatttccaac
     8701 tattagaaag caaagcagcg acagaaagcc gtgcgcacgc tgatcaatcg tccaccagtc
     8761 caagcaccac cacttttgaa caacgggata cagcgacgcc taataacaat ggattgaacg
     8821 ctttacagaa tccatttggg aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8881 atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8941 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     9001 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     9061 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     9121 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     9181 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     9241 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     9301 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     9361 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     9421 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
     9481 tagaaatccc gatcatttta cgcgatcgat gggcatataa aaaaagaaga gttgcaatcc
     9541 taatgtagga ctgcagctct ttttgtttta catagaaaca agaaaaatga aaaggccttt
     9601 ttaggaaggc gcgagcgcct tctcaattga aaaacctctc tcactccaac cgccaccgcc
     9661 gttcgagaag aaaggaagtg aacgttgcac gttcactaag ataaagggtc ttgcaaagca
     9721 agacaaaata gaaaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     9781 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     9841 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgattcatt ctggccaaca
     9901 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     9961 aatcatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
    10021 tcatgcacca catactgtcg aatgcgattg tggacaaaga gcgaaatgta tcaaaagcta
    10081 tcgtttgtat caatgtccaa catgcggaaa aaaatacacc ttgtcttatg gcgattatat
    10141 tttgattgat gagaaaggaa agaaacgctg atgaaaacat aacataaatt attgactgta
    10201 aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagct
    10261 ttacttattc cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg
    10321 ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga aaacatggaa
    10381 acttatgaaa acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg
    10441 gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg
    10501 gaggatatga cattcctata ccatgagaca cgtgttccaa aaaaacatta taaggaaaga
    10561 ttgtcggtag ctgtggaaga aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg
    10621 tactatcgaa cactggaaga actgaaaaaa caaaatgcaa aatggttttt ccaagcctta
    10681 ctatgcttgg aagtaggtgt caaaccaagc actattaaac cttctgaata tcaagcttta
    10741 gaactaacgt atgggaaatt tgtcgaaacg aaaaaagcca agactgtttc aagtgaatgg
    10801 ttagattatt ttgaaaatat caataagtat ggcgcctatt acaccatgaa aaaggaggac
    10861 aacgaaaatg aataaggtga tttatcaact aacaacggaa gatgtgtcac actttttaac
    10921 aaacaaagaa catttgaatg aagtaatcag acggattcaa aaagacttta gttgtatgga
    10981 agaaatgaag tactttattc aaagagtcga agaacaattg tatttggaaa gtgaaacgag
    11041 tcaaacgaag gtcacagtca gcaataatta tttagacgtg tataaacatc gtcctgaaac
    11101 ggtaaaaaaa ggaacggagc ttctacgact agaaacagaa aaatacatca tgaatgttta
    11161 cctagaaagg agtgaactgg tcgaaaactt tgtcagctgc acgatttgca ggaaacacta
    11221 ttactcagaa tcagaatatt attcaatttt tgagagcatg gaattaatgg aagaagaaat
    11281 ggaagagcta aacagcaaaa atgcgacgat agacgattat tatcttgtat ttactagatt
    11341 tttgacgtgt ggaacggaat ctatcgtgtt aatgggaatg gattatgaac tcgctgtacc
    11401 cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg actggtacac
    11461 gactttaact gattttttag atacctacac ttacgacgat acagaagaaa tccaaaatga
    11521 acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaaggc aataatcatt
    11581 tataccgaaa agtgagaatg tgtgaaataa gaacaaaaac cttgaaatta agggaatggt
    11641 tcttatttcg caccaatcgc tccaaccgcc cccgccgctt aggtggtgcg aaataagcgg
    11701 gcaaagcccg ataacctaag agaaagaagg agatccatat gaataaaatc attcctctgt
    11761 tgttcgtact gtgtattggc gagatactta tgcgtcatgg tgcagctgta catttaaact
    11821 tcgtagtatt cttaggaata ctagtatttt gtttgatttt ttggcgcttt gtgttagccc
    11881 ttgtgagtct actattcaag ttgattgcag ccatattttt catagggtta tttcgatgaa
    11941 aggagagagt actaatgcca attgcaatag gaaataaacg gttacccgtg acattagatg
    12001 aaaagagaca aaaagaattg cagcaactaa agcagaagta cggaaaaagt gaatctaaga
    12061 ttatgtgtat tgcgttagat ctattgatcg cgcaagaaaa agcaggattt gatataccag
    12121 cactaaaaaa gtgacgtcac ttttcatttg aagaaacaaa agtgatagca cttaattgtt
    12181 cctactattt agaatatttt ctatgtcctt tttattccat tttgtatcaa taactatgaa
    12241 atctttaaag tatccagatc aatttttttc ttacctgatt ctaaagattt tgttttctta
    12301 aacatattat gatttccttt cgactatttt catataaatg ttaacatagt ttatggattc
    12361 atggaatgtt aatctaattt aatagaaaag gataattaag tatatacgtt ttttcttatg
    12421 tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta cctataaaaa
    12481 aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat cttggaattc
    12541 atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag cggctaatta
    12601 tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa aattacactg
    12661 atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat gaaaaatggt
    12721 gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat ctggacacga
    12781 acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg attatagtaa
    12841 cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat atgtaaatat
    12901 aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac taacgacaat
    12961 tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt tagttgagga
    13021 gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata gaaacagcag
    13081 catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata cggtttgtct
    13141 cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta gatcctcaac
    13201 gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt atagcttagg
    13261 acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct gacgtcgttt
    13321 agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca aatctttaat
    13381 gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac ttgtgatgat
    13441 cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt cgtaatttct
    13501 tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga aatccagtgc
    13561 ttcttgaaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcaa tgaaaattct
    13621 ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat cgatggatga
    13681 gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaaaaca
    13741 ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct agttagggta
    13801 taccttatat agacaaaaac taatgatgga ggaaatagta tgtgtcactt ttctttttaa
    13861 atggcaatat tttttgtaaa taaatgtcaa tcaagaaaat ttaaacgatt atgacattaa
    13921 aaatatattt tatgaatttt ctagtatata acttgattta tctgttaaga tttattaaga
    13981 aaacgttttc ttatttttga tttcgaggtg atagacaatg aaaaataagt ttattttatc
    14041 tttaggtatt ttaactattg gaattagtgt aactggaact acaattccta catttgctga
    14101 tagtacaaat gaaccaacta ctgtagtaga taataaccaa gaattaactc ctttagatag
    14161 gaacggaata gccattggta acccaactaa cgacttaact gttactgaaa attatttaat
    14221 agtttctcca ttacttgctc gagcagcttc taaaacgata cgagtgacag gaacgatgat
    14281 ttttgataaa gttggttggc cttctagacc tcctcaatca cggaattgga aagaaaaaag
    14341 aaatggttat tggtactatg gaaaattgta tttacaaagt tataaatcag ctggaaacaa
    14401 ttggattgcg acttattcag gaaccttaac tagaaagtag ggatttttta tgccagaatt
    14461 tgtgaacaca ccaaattttt atcaagatag cgatattcta agtaaaatta atttaccgaa
    14521 agaagaatca gctttacaag ttcagcgaac tttacaggag acacaaaaaa ttcaaaaatc
    14581 aatttctaat ctagaagatt ctgaagaaaa aagaaaaaaa agagaccaaa aaacatatca
    14641 aaaagaaaaa taatgatttt aaaaaaagag tgtctagtcg acattctttt tttctaattg
    14701 aaagtgttca taaaatcacc catttctgga cactttcttt ttttgcttta tttttcaaaa
    14761 aaacaattca tttcatgttc ataaacccat aacaatatct atgttcagtt aaactatatg
    14821 gataaagtta tgttacaata aaattaatga aggagtgatt tttttgaaaa taggatacgc
    14881 ccgggtatct actggtttac aaaatttgga tttacaagaa gacagcctaa ataaatttgg
    14941 ttgtgaaaaa atttttactg atcatatgag tggggcaaaa agtaatcgac caggattaga
    15001 aatggccatt gattttgttc gctctggcga tacacttgtc gtttggcgct tagatcgttt
    15061 aggtagaaat atggaagatt tgatttcaat cgtcaatcga ctgaatgagc gtggtgtgag
    15121 ttttcatagt attcaagaaa atattacgat ggacaaatca agttctacag gacagctcat
    15181 gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat ttgatcctgg aacgatccgc
    15241 agcaggacga gcagctgctc gtgcaagagg acggtttggt ggtcgtcctg aaaaattgtc
    15301 tgaacaagat ttggaattac tgaagacttt agtggatagc ggtacaccta ttaagactat
    15361 tgcggaacgg tggaacgtct caagaaccac catttatcgt tatttggcaa aaatggagga
    15421 gaaaaatgga aagacaaatt ctcattagtt tttgtcattt aatgctgaaa atttttgcca
    15481 tttatctaga aaagtgacaa tagtatgaga attttttgta agtgtctact gataacttat
    15541 tctcaaatgt tgtaaggatt ttattcattt gttaatatta aagaacggaa ctccaaaaaa
    15601 gtccgtactc taatttgcag tctctcactc ttttagagtg agagttttta ttagattttc
//
LOCUS       DAYYFD010000068        15500 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000068 DAYYFD010000000
VERSION     DAYYFD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.068"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..455
                     /locus_tag="ACHA25_002044"
     CDS             105..455
                     /locus_tag="ACHA25_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8763330.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            554..1084
                     /locus_tag="ACHA25_002045"
     CDS             554..1084
                     /locus_tag="ACHA25_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8763331.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1238..2389
                     /locus_tag="ACHA25_002046"
     CDS             1238..2389
                     /locus_tag="ACHA25_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8763332.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2498..4537)
                     /locus_tag="ACHA25_002047"
     CDS             complement(2498..4537)
                     /locus_tag="ACHA25_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8763333.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(4595..5152)
                     /locus_tag="ACHA25_002048"
                     /pseudo
     CDS             complement(4595..5152)
                     /locus_tag="ACHA25_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(5300..5466)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(5691..5897)
                     /locus_tag="ACHA25_002049"
     CDS             complement(5691..5897)
                     /locus_tag="ACHA25_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763334.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5913..6527)
                     /locus_tag="ACHA25_002050"
     CDS             complement(5913..6527)
                     /locus_tag="ACHA25_002050"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8763335.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6674..7357)
                     /locus_tag="ACHA25_002051"
     CDS             complement(6674..7357)
                     /locus_tag="ACHA25_002051"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8763336.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7466..7924)
                     /locus_tag="ACHA25_002052"
     CDS             complement(7466..7924)
                     /locus_tag="ACHA25_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8763337.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8008..9771)
                     /locus_tag="ACHA25_002053"
     CDS             complement(8008..9771)
                     /locus_tag="ACHA25_002053"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8763338.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9992..11452)
                     /locus_tag="ACHA25_002054"
     CDS             complement(9992..11452)
                     /locus_tag="ACHA25_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763339.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11660..12586)
                     /gene="glsA"
                     /locus_tag="ACHA25_002055"
     CDS             complement(11660..12586)
                     /gene="glsA"
                     /locus_tag="ACHA25_002055"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8763340.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12609..14036)
                     /locus_tag="ACHA25_002056"
     CDS             complement(12609..14036)
                     /locus_tag="ACHA25_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8763341.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14067..15281)
                     /locus_tag="ACHA25_002057"
     CDS             complement(14067..15281)
                     /locus_tag="ACHA25_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8763342.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4427 a         3096 c         2664 g         5313 t
ORIGIN      
        1 ttttgtcaca atcctttttg tttttttgaa taatcgcttt ttcttctctt tttcttatat
       61 actataagta gtaaataatt tgaaaaaaag aaaggtgact gattatggcg caaaatcgta
      121 tttttacgac aagttttgca agcgtttatc ctatgtatgt agcaaaagcg gagagaaaag
      181 atcgtataaa agaggaagta gatgaaatca tttgttggct gacgggttat tcccaaacag
      241 aactagaata tcaactacaa aaacaagtga ctttcgaaca attttttgcg gaagcccctg
      301 tccccaatcc ttcacgttcc ttgattacag gtgttgtttg tggtgtacga gtagaagaca
      361 tcgaggataa cttgatgaaa gaaattcgtt atttagataa aatgatcgat gagctagcaa
      421 aaggcaaaaa aatggaaaag atcctcagaa gttgattttt ccagagatat tcttctcctt
      481 cgctcatttt gtaacattta atttggtaaa atgactagtt cacgttaaaa taagtcaaag
      541 gaggttttct tatatgtttg agacgattaa acaaaatatt caaaaatacg ccttattaag
      601 agctgctgtt tatattattt ctgggattgc gattgtgcta aacccttctg ctgtttttca
      661 ttttatcggt tatctgatca ccgcctatct tgttttgctt gggttgttga atctttttga
      721 ggcttaccgt tttaaaagga aaacaggttc ttggagtatc ggtctgttca gtggtctttt
      781 tttcctgatc ttagctctga tcgtctatgc ttttgcacct gcaatcgttt ctatcctgcc
      841 gattcttctt ggattagctg ttatcatcaa cagcttgttc cagttgttct tcagcatgaa
      901 tacaaaagct aaaagttgga gtgtttatag tagtattctg ttgttgattg gcggattcgt
      961 cttattgttc aatccattca aaagtttgat gcttctattc caaatattcg gctttatttt
     1021 gattttcatg ggcatccagg agattatcaa tttcatcaga ataagaaaaa tgactatacg
     1081 ttaatcattt ttagttatta aaatagaccg tataaaaacc aacaatccaa aaaaatcttt
     1141 tggattgttg gtttttttgt gagtatattt tcctattttt tcaataatgt atgttatgct
     1201 aagttcgggc tatattttat acaaaggaat gatgagaatg gaatttattg gtattttatg
     1261 cctgatattg atttcaacaa cgattggctc acatatctcg cggagattag gtatcccagc
     1321 agtgattgga caattacttg ttggtgtttt gctaggccaa gctggattga attgggtcaa
     1381 acctgatatt ctagtccatg acttttcgga gattggtgtg ttcttattaa tgtttttagc
     1441 tggtctggaa agtgatttat ctcttttgaa aaaatatttc cgacctggta tgttcgttgc
     1501 tttgcttggg atcatcttcc cggttgtttt tggctggttg acaggcaaag cattccaagt
     1561 ttcgggaaat gaagcgattt tctttgggat tattcttgcg gcaacttctg tcagtatttc
     1621 agtagaagta ttaaaagaac taaacgtagt gaatacaaaa gaaggatcaa caattttggg
     1681 tgcttctgtg gtagatgaca ttttagtcgt tttagttcta agtttcagcc tatcttttct
     1741 aacgggggaa gcttctagca atgcttcttt accgcttgtt ttgattgaac agctaattta
     1801 ttttgttttt attttcttat tagtcaaatg gattgcgcct ttcctacttt catttgcaga
     1861 aaaagtgtac gcaaattctt ctattatcat cgtatcgcta gttatttgtt taggaatgtc
     1921 ttacgtagcg gatcttgttg ggcttagctc tgtcattggt gctttttttg ctgggatagc
     1981 tgtcagtcag acaaaggtta aagaagaagt ataccacaat gtagaggctt taggatatgc
     2041 agtatttata ccagtatttt ttgtcagtgt aggattagaa gttgattttt ctgactttag
     2101 tgaacaacta ctctttattt ctatattgac gcttgtagcg attttgacca aattgcttgg
     2161 aggttatttt ggtgctcaga ttgccggttt ttctcaacat agttcattga tggtcggtgc
     2221 tggtatgatt tcgcgtggag aaatggcttt gatcatttta caaattggac agcaaagtca
     2281 attgatcgaa ccacagtact attcaccttt agttatcgtg attttattaa gtacacttat
     2341 ttctccactt gttctcaaat attttacaaa aaaagtttat gaagtgtgag taaatcaaaa
     2401 agaagttgtg aaagagacat cgtgaaaacc gtttatttga aaaaaggact gtgacgataa
     2461 cgccacagtc cttttactgt acttaaaaag caatttttta ttgataattt tggttgaggt
     2521 attgcaatag ttccgatgct cgtttggttg ctgaatcatc atcatcttta ttttccaaca
     2581 tgctgaccat catataacct tggttatcaa gattgaaagc aaacaagaaa ctgttctctt
     2641 gcccctttac atcctgtttt tctttgattt ccgctgtacc agttttcgct gccaacggaa
     2701 tccctaaagc agaaagagaa tgtgctgtac catttacatc ttgcacaact tctctcagat
     2761 ctggcacgat cgtttgtact gctgtttcgc tgatgacatt cttcttatct tttgtctctt
     2821 tatctgcaat caatttagga tagacaagtg tgccattgtt ggtaaaaaca gaatacattg
     2881 ctgcttgctg gataggagta attagaagtt cgccctgtcc atatccagta tcagctagca
     2941 agatatctga gttaaagcta tcttcattag aaatttgtgc tggattcata ctaattggca
     3001 aatcaaggtc ttcaccaaaa atgaatttat ctaagcctgt acgaaatttt ttctcaccca
     3061 ttttcaacgt ttcttgtgcc gcatatatat tatcggaata gatcaaagca gtttttaagt
     3121 ctacttgtga tacatcacta ctgacacgcg ttgttttaaa agatccccaa gaactgtctt
     3181 tttgccattt aagcccgttg atcgtcaaca cttcatttgg atcgatagtg ccgttgtcga
     3241 gaccgattgc tgctgtaatt attttaaacg tagatccagg agcataacct gtcgcaaatc
     3301 ggctgatgaa tggatgttca ggattttctt cataagcttt gtaatcttct tgtgagatcc
     3361 cgtttgtcat tttgtttgga tcatagcttg gagagctagc aagcgcaaga agatcaccgg
     3421 tttttggcgt tgtcgcaaca gttgatccag cttttcctcc tagactgtca aaagctgttt
     3481 tttgtgcctt cgcatcgatt gtcaatttga tatctttgcc attttgaact tcatgttcga
     3541 tcagaacttt tttctctaca ccgtctgcat ctgtaatgct taatttctca cctgttgtcc
     3601 cacgaagatc cttatcaaaa gccatctcca aacctgagcg accgattttt ccattactgc
     3661 ttaattctgg attcttatca atatcttctg ctgtgatatc tcccacgtaa ccaatcagct
     3721 gagcagctgc ttcacccaaa ggataatatc tgccgtctac ttcttgaatg gtagctccag
     3781 ctggaagttc tggcgttgct ccatcaatga ttttcaatgg gacaaagtaa tcgggttgta
     3841 cccagctttg tgaaatcgcc tgattgatag catcttctgt taagtcgaag gaggaagcaa
     3901 tcgctttgat attggctgtt ttttcgcctc catctccaag tttacttgga acgactccta
     3961 attgttttag tttgcctgtt gttgctaatg gttcaccatt tcgatctata atattccctc
     4021 ttgctgcttc ttgtgtggtc aggcttactt tgtcatttcc ttccatttct ggaaaaacca
     4081 agttgggttg ccagttgatc gtggtttgac catcatttct gtctaatgtt cctttataag
     4141 aaagatcttt caattcacct aatgaggtat tcatctttgc tttatatgaa aaactgtaag
     4201 tagaatcgtc ttttttatct acttttagat tcgatatctg aagtcctttg acatcagcag
     4261 caccgtatat attctggtat tgatctaaga tctctttttc agataatgca cttttatttg
     4321 ccgccttttt tgatgtcatt tctgcagctt tgttataatc tcccttgttc aaagcttgga
     4381 caaattgctc aactgtcttt tctccagctt ctactgcttg ggtttcttga tagtgctggt
     4441 agtaaaaata accgccaccc gcagctgcta ttaagatcac tgcgccggca atataagcgc
     4501 ctgttcgatt tttgccgtgc ttgtcacttc ttttcatttt ttcctccttc tcttgcttgt
     4561 cattccttta tttcactatt tatacctgtt tattctaaca tatgtctttg aggaaatcca
     4621 taccacaatc cagaagcaca ctttctggaa ggttcgtttg gatactacca agtaatttcc
     4681 caaccgcttt gggtagcttg aatattgaag ttacgtcttt cagctgctga gagatagctt
     4741 tgatcacttg ctgctgcctt ctgatcctcc cgaagtctcc ttctttgtct tcccgaaatc
     4801 gagcatactg gagtaaagta ttaccatcca tcacttgctc tcctttgttt atatatacgc
     4861 catctaaatc tagagatttt tctgcatcga tcttgactcc accaggaaac agttcattaa
     4921 cgcaatcgat aaaatcttga aatgtgatac ttgcataata gggagcttca aatttgaaat
     4981 tttcctttaa tgtttgattt aataaatcaa ctcccccata agagtaagct gcatttatct
     5041 tattcattcc gtaacctgga atatcgacat atgaatctct catgatagag atcagttttg
     5101 cttgttgtgt tttctggttg tattgtgcaa ccagaagtga atccgagcga cctccaccaa
     5161 tcgatctttt ttcaccgcat ctcctcctca cacagcaatc cacttgtcag gtgaggagcg
     5221 agtttgtctc atcaaataag tcagtcacaa aaatataaag aaaaggtcag aaaccttttc
     5281 gaacaagaat tctgacctag catcatctca tcgtccgagt tttagcacta tacaacgtaa
     5341 agaaaacatt cttagctatt ggaaaaagcc ttaatccaac agtttctgtt ttacccattg
     5401 gcgactttcg acgtttctgg gcaacagcct atgtttgtat aggagcctca cctaacaagg
     5461 ttattactat attctattta cgagttcatg atagactttt cctaatgatt aggcaatgga
     5521 atttgaaatg aaatagtgaa atttaacaat ctttaatatt ttttagaaaa aaataacaaa
     5581 taagttgaaa gaatcccgtt gcaaattctt tcagttgcac caggattctt tgataatttt
     5641 ttagttttat gtgttttttt atatgttttt tcacttttcg gaataaatat ttatttgtta
     5701 aagtaattaa acaatccgct aatcgtttga tctggcattt ctatttcctt accatctaaa
     5761 gtaacagtag taacgacaaa atggtcttgc aatggcggaa cttctaagta ctgcccttgc
     5821 agaaaatcat tcactgtttc atattctttt ttcatttctt ttgaatctgt cgatggttta
     5881 tagcttattt ttaacataac tagtgctcct ttttattcgg gacgtgcaat cgtgaaaatt
     5941 tctcctattg ctgcataatc atttcctgcc aaacggctta cagcattcaa ttttcttgga
     6001 tcgatccttc ctgtcttatc atcatagact gtttcattta aatgatattt ctgtacttct
     6061 agcaaaaata aatcagctgt tactttattt ttattccgta tctcgacatg ttcatataag
     6121 gatgtttcaa aacgaacttt tgcttctgtc aatgctggta ctgaaatgat tactgaatca
     6181 gttaaagtca agtcagtcag gtacagttcg ctttcctcat taggcagatt ggctgctgtt
     6241 ttatttactt cttctacgtt gtcctcatca aggatatgga taacagcttc tcttgtctca
     6301 agaatattct ttgctgtgtc ttttactgca tcttgttttc tctggatcgc caaggataag
     6361 atgggtcgat tcgaactgac gatattgaag taactgaagg gtgcgatatt cactatatct
     6421 tttgctgatt tagtcgttac tactgctact ggtctaggga tgactgaacc aatcagcaac
     6481 ttgtaatttt cacgttctgt caattctgaa ggactccgtt caaacatggt ttcgccttct
     6541 ttctataaaa ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa tgcttttttc
     6601 atcttcttag aatctgcttt aaactatcta tcaatttttc ttttttgtca agaatctgtg
     6661 ccgtgcgtgt tttttagtta tttgctttac ttgcggcttc gacatatctt ttagtgatca
     6721 attgcggacg ggtgataata gatccaacta cgactgaaaa agcacctaat tcatatgctt
     6781 tctctacttg ttcaggagag tcagtatgtc cttccataat tactgggata tcgacttgtt
     6841 ctatgatttg attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa tattctgtat
     6901 agcctgctaa agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga agcgcttcct
     6961 caaaagtcga aatatctgcc atagctaacc gaccgtgttc gtgaatcatt tggacaagtt
     7021 cagcagttgt tttttctgtc ggacgtttac gagaagttgc atccagtgca atgatttctg
     7081 cttgcgtctc caaaagttct tccacttctt tttctgtcgc agtaataaac acttctgacc
     7141 catcgtaatc tcttttgata atccctatta ccggtaaatc aacgattcgc ttgatttcat
     7201 tgatatcttc tgtcgtattt gctcgaatac caactgctcc gccttctttt gcagccaaag
     7261 ccattctccc cataataaaa gaactgtgta gcggctcgtt ttccaaagct tgacaagaaa
     7321 cgacgactcc gcctttgatt tgttccagtg tatccacaat atctcctcca gtaatgttat
     7381 ataaaaatac agcttcatat aaaaatgtaa cccttttcta ttcaggagac aataatgagc
     7441 tatttctttc agcagacctt tcttactaaa agaaaattac ttcaaattct tcgtatacga
     7501 ccagcttctc ttctgtaaat tctatgttca acgcttcttt ataagcatgt ttaaaaaact
     7561 cagcatggac tttccgccaa taagctaatg tccgatcccc ttctccttct ttccaagcat
     7621 gctctttcgt gacttcacta aaaggcaaga tggtcacatt tatatttttg atgatacaaa
     7681 ctggttcatt ttttccattc aggaccacgt ctaattgacc aacttttggt aaaggctcat
     7741 tctcttgttt atacagatcg taaccacttg atgtaccagt ctttatcttt tttacaacta
     7801 gttcgctcaa ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa cctaattttt
     7861 ctgattgata actaggatat ttttcttgga actttttgac atattcttct acttgctgat
     7921 acataacatc cacccctttg ctttttataa tgaaaaaacc atccagatac tagtataaac
     7981 aattatctgg atggtgagca taatgaatta ttgaatattc aagacacgca gactatcttc
     8041 aacgatttgt tttggtgtta aatgataacg ttcataaagc tgccctacag gaacgctatc
     8101 cgtgaattct ttctctgcac caaaatttaa tacgttcatt gaagaatcac cataataacg
     8161 actgatattt tcgccaaatc ctcctgacaa actaccatct tctaatgtga tgactaattc
     8221 gtgtgtttct tttaatttat ctaatgtttc tacgtcattt tctgtgatcg ttcctggatt
     8281 gatcaacgtt gcttcgattc ctttttttct aaaagctttt actgtttctt ttcctaattc
     8341 aagaaaaccg cctaaagcta aaattgctac ttttcctcca ttatttactg tttgataggc
     8401 tggaatacta taatcatttg cgatagctgt accatgttct acaccatgtg taggaattcg
     8461 gattgctaca ggctgcgctg tttgttcgaa cgcccaatct agcatagaga tcatatcctc
     8521 ttttgttgct ggagcaagat atttgatgtt cgggatactt gataagtaag gaatgtcaaa
     8581 tgttccttgg tgtgttcggt cactagctga gatcgttccg ccaccgacta aaataatcac
     8641 tggattctcg ttgatcgcta aatcatgtga taattgatca tatgcccgtt gcaagaaagt
     8701 acttgattgg aagacgactg gacgtactcc ctgagaagcc aaagcagaag cgaaagtgat
     8761 agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt cttggaatgt
     8821 tttcagtcca aaggcgcctg gtatcgctgc attgactgcg gcaataggag ctgtacttgt
     8881 ttgcattttc ttatccaaat aatccatgat gatcatatta aatgtttctc cagaagttac
     8941 ttgctttgcc tccccggtgg acagatcaaa tggtacatgc caatgatatt tcattttatt
     9001 atcgattgcc ggctgataac cgcgaccttt ttccgtatgg acgtgaagta caatcggatg
     9061 atcaatatct tttacttctt caaataaacg gatcaccgtt tccagatcgt ttccattatc
     9121 cacgtaacga taatcaaatc ccattgcttt aaataggtta ttgctgcttt taccgttggt
     9181 ttgacgaagc tcagctaagt ttttatataa actgccatga ttctcatcaa tagacatctc
     9241 attatcgttt actaatacga ttagattgga ttgtaaaaca ccaccattgt tcaatccttc
     9301 aaaagctaag ccaccactta gggagccgtc accgataaat gctaccacat ttcctgattc
     9361 tttcttcaga tctctcgctt tagctaaacc tgttgctaag ctgatagatg tagatgtgtg
     9421 ccccactgta aaatagtcat gtttactttc atgttggttg gaatatccgc tcacttcacg
     9481 gtaatgtcct ttttcaaaac cattttttcg accagttaaa agcttatgac cataagactg
     9541 atgggaaaca tcccaaacga ttttatcctt tggtgaatca aatacataat gaaacgcaat
     9601 cgttaattct actacaccta gattcggacc aacgtgccca cctacatcgc tgtccttttg
     9661 caagattttt tggcgcatct cttctgccaa ctgtttcatt tcctcaacag ataatttttt
     9721 gacatctgcg gggctattga ttgcatccaa tacatgtaat actgtactca taatacattc
     9781 tctcctccgt actatcttct tttaatgaaa atagatttgt tacactttat ctattctact
     9841 acttagagtt gactctaagt caatagcatt ttaaaaaaat ttcagagaaa gcttgttata
     9901 taagtagatc ttcgcacgaa tcaggactca aataaaaacc ttctacagac aattaaaaag
     9961 ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat atgttttttt aataggtgaa
    10021 gttcctgagc aatatgatac aaatggatgt ttgcaccttt taatgagatt ggatagatct
    10081 ttgtcttttc agtttctaat ccattttgat agctagcagc atcgcacaaa accactcttt
    10141 ttttcgcaca tagacattca tcaggcaatt cttctaatga acgaaataaa ataggattta
    10201 tttcagcgat gccttcttcc gcatacaaat gaaattgggt tcgataaaaa aattgtttga
    10261 ctgaactttc tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt tcactgttga
    10321 tcaaagaaag acttttgacg aattttcgaa tgaaattttc attccatctt agttggattt
    10381 gatttttctt tttccactct tccaatattt ctacaatttt tccgtacaga agttgttctt
    10441 tttctgatag aagatagatc ctttccggta aaaatacttg tccatctgcc cacaacgtac
    10501 ggacaaattg taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg atttcagcga
    10561 tactaataat ctgtaaaatc aattcattca gtgataggtc attgagaaca aaatttttat
    10621 aaacaacttc gaaatctttt cttaacagct ccatgttctc attggtataa tttctactgt
    10681 tgaacaaaga aaaaatataa tagacatctt cttcttgtaa gtctagaatt cctttttgct
    10741 cttccaatcc ttgacagact aattgaaata acggcttcgt ttctgcgctt ttcttatctt
    10801 cttctttcag tccaagggga aatgcctttc ttccgattgc gatctctatt cctcgtatga
    10861 taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct acataatcaa
    10921 tcaactcgtt acaagcttct tctacatacg gatttttatt ttcatagcct tcccaattga
    10981 cataacgtac taaagctagc aagatgttcc gcgagtcaaa ttctgggata atgatttctt
    11041 tatttttcaa ataatcattt tctttaaaaa aatccaatac gatttttttg attgtatata
    11101 ctttgctcaa agaaataaat tcaagatcag ccagttctgt actcgtgaat ataccattaa
    11161 aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt gtgagctcag
    11221 aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt acttgttctt
    11281 ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc gttggtgtag
    11341 tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca aataattgtt
    11401 cacacagata cacttttcta aatatatttc gctcgatgta attttccagc attcctgata
    11461 cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt atttttgttt
    11521 cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc
    11581 aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg acttttaccc
    11641 cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg actacatctt
    11701 tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg ctgaagacac
    11761 ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg ctttttgcag
    11821 gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca gttgtcataa
    11881 ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc caaggagcaa
    11941 ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg acattgaggg
    12001 agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc atttgattac
    12061 ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca gaatgataga
    12121 tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat gacaagattc
    12181 gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca atcgctcctg
    12241 cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc atgatagaat
    12301 tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct attccgttgt
    12361 ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca aagcgcacat
    12421 cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc atacctaatt
    12481 gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct gtttgataat
    12541 agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga ataaccagct
    12601 ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat cgtaaatgga
    12661 ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc atgctgtcca
    12721 gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga agtcaaaaga
    12781 cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata ggctctttca
    12841 agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa gtacaacatc
    12901 aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt ccccccgccg
    12961 cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat catcaaatga
    13021 gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc ttcttctgct
    13081 gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc gatgacacct
    13141 aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc aatgtgtctt
    13201 aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg ttgaggtact
    13261 gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat aaccaataca
    13321 aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg actggcagat
    13381 gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt aggcaatttc
    13441 gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc gcctgaagcg
    13501 acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc agctacaaaa
    13561 ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag taaaatagcc
    13621 cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat agcaccaata
    13681 cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta gccaactgtt
    13741 atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa tgtgcgactg
    13801 acccaagtat agacaccgcc tttttcccat cctttcaccg ttgccatctc tgctgcgcac
    13861 aatgccacag ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag
    13921 cctgaggtcg cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc
    13981 aaaaagccaa agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat
    14041 cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc
    14101 aatccttctt cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt
    14161 aaaatcactg ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga
    14221 atccctaaga agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc
    14281 cctatcagaa tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca
    14341 tcatcccatt gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg
    14401 atcccaatca gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc
    14461 aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt
    14521 gcaatgatca gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca
    14581 ttgactgctt gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac
    14641 caaagcagtc ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct
    14701 aaaggaatct ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc
    14761 gtgtggatga ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca
    14821 ccccatatcc agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata
    14881 atatagcctt ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc
    14941 acgataaaca tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac
    15001 atgttgggga agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca
    15061 ccgccgatat cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt
    15121 atcccaacgg agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat
    15181 ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc
    15241 atgaatgctg tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta
    15301 cgattacgag tataaaagaa ggtgtagttg tttttttctt ctgttttttt gaattaatac
    15361 ttgcatttat aaaagacaga caattaataa aagcttgatg tgttgtgctc tttttcgttt
    15421 ttcacacaaa taaagacaaa aaataaacgg tcttcttttt atagaaaacc gtttattttc
    15481 gtcttttttt atttttaaag
//
LOCUS       DAYYFD010000069        15333 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000069 DAYYFD010000000
VERSION     DAYYFD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.069"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..822
                     /locus_tag="ACHA25_002058"
     CDS             31..822
                     /locus_tag="ACHA25_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8763343.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            788..1741
                     /locus_tag="ACHA25_002059"
     CDS             788..1741
                     /locus_tag="ACHA25_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFM8763344.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2139..3113)
                     /locus_tag="ACHA25_002060"
     CDS             complement(2139..3113)
                     /locus_tag="ACHA25_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8763345.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3228..3662
                     /locus_tag="ACHA25_002061"
     CDS             3228..3662
                     /locus_tag="ACHA25_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8763346.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3787..4809)
                     /locus_tag="ACHA25_002062"
     CDS             complement(3787..4809)
                     /locus_tag="ACHA25_002062"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFM8763347.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5201..5623
                     /gene="arsC"
                     /locus_tag="ACHA25_002063"
     CDS             5201..5623
                     /gene="arsC"
                     /locus_tag="ACHA25_002063"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFM8763348.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(5903..7825)
                     /locus_tag="ACHA25_002064"
     CDS             complement(5903..7825)
                     /locus_tag="ACHA25_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8763349.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(7912..9852)
                     /locus_tag="ACHA25_002065"
     CDS             complement(7912..9852)
                     /locus_tag="ACHA25_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8763350.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(10140..10880)
                     /locus_tag="ACHA25_002066"
     CDS             complement(10140..10880)
                     /locus_tag="ACHA25_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFM8763351.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(10893..11984)
                     /locus_tag="ACHA25_002067"
     CDS             complement(10893..11984)
                     /locus_tag="ACHA25_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFM8763352.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(11974..13080)
                     /locus_tag="ACHA25_002068"
     CDS             complement(11974..13080)
                     /locus_tag="ACHA25_002068"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFM8763353.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(13099..13761)
                     /locus_tag="ACHA25_002069"
     CDS             complement(13099..13761)
                     /locus_tag="ACHA25_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFM8763354.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            complement(13772..14551)
                     /locus_tag="ACHA25_002070"
     CDS             complement(13772..14551)
                     /locus_tag="ACHA25_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFM8763355.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(14580..14930)
                     /locus_tag="ACHA25_002071"
     CDS             complement(14580..14930)
                     /locus_tag="ACHA25_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFM8763356.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(14946..15302)
                     /locus_tag="ACHA25_002072"
     CDS             complement(14946..15302)
                     /locus_tag="ACHA25_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFM8763357.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         4542 a         3235 c         2614 g         4942 t
ORIGIN      
        1 attgactttt atgttaaagg agtaattcat atgctgaaaa aagaaagaca agaacaaatc
       61 attacattat tagagaaaga tttttattta actaattcag aaatcgctaa caaattaggc
      121 gtatcagaaa tgaccatacg ccgagatgtc actgaattag caaaaaaaaa taaagtaaaa
      181 aagctatatg gtggcgttga acgaaaggaa ccttataatc gagaactatc gactcaagaa
      241 aagattgata cgaatgtagc cgaaaaaaaa tatattggtc aagtcatgaa cagtatgatt
      301 cccgataatt ctactattta tgtcggtgct ggaacaacga tcctatatgc acttcctttc
      361 attcaaaaga aaaatctatt tgtaattaca aacagtttga ttgctttcaa ctatttaaag
      421 aatcatacaa gttatcggat tttacttact ggtggcgaat tttctcctat tacagaagag
      481 ttcattgggg aagttgcaga aagatctttt gaaagtttga atgtcgatat ctctttcgct
      541 gcaacaaacg gcatatttga taacaatgtg acaaccgctc aatttattga gggaaatatt
      601 cagaacgcag cgctcaatgc agcaaaaatc aaatgcattg ttgcagatta ttcaaaatta
      661 aataaatcag acgtctatac ttttagaaaa ttaaccgatt ttgattatct aataaccgat
      721 aatcatttat cagaacagga tttcaatcat tattcaaaat acttaacaat attaaaggag
      781 cccaaaaatg atactgacaa tcacgatgaa cccatccatt gattccattt attttacaaa
      841 acactttgaa ttaggtacga tgacacgctt tgattctccg gtacgctatg taggcggcaa
      901 gggaatcaat tgcggacgag cttctgctct actaggttca tcagttattt taacagggtt
      961 tttgggcggt tattttggca gtatgattca agatatgctg aaaaatgaaa atttgtttga
     1021 tttgcatttt ttacctattg cagaagaatc aagaggtgcc gtcacgatca tgcatgatga
     1081 tgaaacgcag acagagctag tggaagctgg tcctgaaata gcagatacta ctatcaaaaa
     1141 attaatgaat gatatcaaac taatcagtaa aaataattac ctttcagtca ttacaataaa
     1201 tggttctgtt aacaatccag atcccttttt gtatgctaaa ttattacaat atatcaaaga
     1261 acaaatagat ccttccacct atatattaat ggatatatct ggacaacatc tccgtgaagt
     1321 cttagcaagt aagtataaac cgatgttcat caaacctaat atccatgagt tttcagatat
     1381 catccatcaa aaaattactc aaaaaagcga cataaaacac catctaaaaa caaatccact
     1441 atttaaagat atcgatgtta tcatgatttc ttgcggctcg tatggtgcag tagtcaaagt
     1501 aaaagaagaa ttttggaatg ttgagatccc taagattcct atcattaata caacaggctc
     1561 tggggatgcc actgttggag gttttgctca ttcattagaa cagaattatc ctttaaaaga
     1621 atgtgtaagg tacgcaatgg cttgcggcat gtcgaatgca cagcaatcag aagtcgggaa
     1681 agttattcca gctgatgtaa aaaatttagc aaaaagcatt aaaatcacag aaatgaaata
     1741 aatagtacaa agtaaataat tgtttatatg aaatgagaca caaagagact gtgacaaaaa
     1801 acgtgtgtac cgacccccaa aagttagatt ttttggtcta acttttggga gtcgatacat
     1861 tgtcacagtc cttttttcga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     1921 caatattcaa caaacatgag aagctatttt ttgaaattgc tccttgtgac tcagagttaa
     1981 acggcaggct cacgacctct tggtataaac ggtgttcaaa agcttgcatc tacgctaatg
     2041 agtccaacca agtcaaaaat gaggagcttt tttggctgtc cttattactt gatgcagggc
     2101 agttttttca taatctcttt tagattaaat ccttttggtt acacaaggtt ctcttcaaag
     2161 aatgttgcca ttttttcaaa tggaatgatg tctgtctggt cgtatagatc tgtatgggat
     2221 gctcctggaa tgatcatgag ttccttgttg tcacctttta gttctttgaa agcgtcctcc
     2281 ccaaagtaac gtgaatgagc tttttccccg tgaatgacta agactgcatg acggatttct
     2341 ggaatataat gcaatagttt tgcattcata agtgacaacc ctgatgtcct attccaacca
     2401 tcattggagt ttaaagaacg gacatgatat ccgcggtctg ttttataata cgcatgatag
     2461 tctttgacaa attgaggagc atcttcaggc aacggatcaa ccacacctcc agcacgtgcg
     2521 tagtcccctt gtttgaaatc aatgatgcgt tgtgcattta attcttcacg catttcatat
     2581 cgggcatttt catccataac atcgaagtag ccatttgctg ttactcgagt catgtcatac
     2641 attgtggaaa caactgttgc tttgacccgc gtatcgatag ctgctgcatt caatgccata
     2701 cctccccatc cacaaatacc aatgattccg attctctcag ggtttacatt tttttgtaca
     2761 gataaaaagt ctactgccgc ttggaaatct tccgtattga tatcaggcga agcaacatat
     2821 cgaggtactc ctccgctttc tcctgtaaac gatggatcaa acgcaatcgt taaaaagcct
     2881 ctttcagcca tcgtctgcgc atatagacca gacgattgtt ccttcactgc accaaatggc
     2941 ccgctcacag cgattgccgg caatttcttt tcacttactt cttttggttc atataaatca
     3001 gcgactaatg tgataccaaa acgattcggg aagctcacct ttcgatgatt gacttgattg
     3061 ctttttggaa atactttgtc ccattcttgt gtgatgttta attctttatt catattctag
     3121 actctccttt gatcaaaatt gacatttgtt tttgaatgta ttattatgat aaaacttaaa
     3181 cttgacttta ggtcaaggta aaaattttat tttaggagga tttttttatg tattccattg
     3241 gacaagtgtc tgaaatgttt ggattgcctg tttctacttt acgttattac gacaaagagg
     3301 gattatttcc tgatatccag cgtgtttctg gtattagaaa attttcagag aatgagttag
     3361 aggctttaag agtcattgac tgtttgaaaa aatctggttt agaaataaaa gacattaagc
     3421 aattcatgga atggagcaaa gaaggcagta agacatttga ggttcgtaaa gaactatttg
     3481 aaaaacaaaa agaagtagta gaaaaagaaa tagcaaaatt agaaagagta ctcgctatgc
     3541 taaactacaa aagctggtac tatgaagaag caataaaagc cggcaatgaa gaagccgtat
     3601 tgacaatgat tcctgatgat ttaccccaac atgtaaaaga agcttatgta cattctcatt
     3661 gaacaaacgg ataactcact ctccttctct atgaacttca tgaaaacatg aataaacacc
     3721 tctaactgat tgttagaagt gtttattttt tcactcaagt atcctgcact tacaaaaata
     3781 tcctttttac cgccatccta attctggagc gatatctttt acgattgttt caagcaagtg
     3841 catgttatat tctaccccca aagtattagg aatcgtgaca agtactgtgt ctgcttcttt
     3901 gattgcttca tcttcagcta gctccttgat taattgatca ggttcaccag caaaagtttt
     3961 tccaaaaatc gttgggtttc ggtgataagc aagatagccg acagcatctt gttgcggact
     4021 accgtcttgt ccaaataagc gtcgatccaa atcagtggtg attggctgaa tcgagcgagt
     4081 aactaatgtt cttggttcaa aatcatgacc tgcttgcttc cacgcttctt tatacgcacg
     4141 taattggttt gcttgttgga tatggaatgg ctcgtgggat tcgtaattga ctaatgtaga
     4201 tgattgcaga ttcattccat gttgtgctgc ccagattgcc gtttgctgcg aaccagctcc
     4261 ccaccagatt ctttgtcgta atccttctga ataaggttct atccgtaatt ttcctgatcc
     4321 ttgtgggaac attggttgag gatttggttc ttcaaatggt tttccttcaa gtaattccaa
     4381 gaattcaagg gtgcgttccc gtgttacctc tttgatttct tcttcactgt aatcataacc
     4441 aaagtatttc cagccatcta gaacttgttc aggtgaacca cgccccactc ctagttgcac
     4501 tcttccttgc gtgatgatat ctgtcaatcc ggcattttct gccatataat aagggttttc
     4561 atatcgcata tcaataagtc ctgtaccgat ttcaatctta cttgtctttg cgccgattgc
     4621 cgctagcaaa ggaaacggcg acccaatctg aggggcaaaa tgatgtacgc ggaaaaacgc
     4681 tccgtccact ccgatttttt ctgcttccac cgctaaatca atcgattgca aataagcatc
     4741 ttgagctgat ttcacaagcg atccttgctc catccaatgt ccaaagctta aaaagccgat
     4801 ttttttcaag gtaatgacct cctttaaacc tacaaaaatg gaaggttttt tatttttaat
     4861 atgaactgat tctactactt tttttcttaa aagacgaata attgttctcg cctcacaatc
     4921 aagaaatgta tcggcttact acacccctac tttcttcatc atctgccgtg tcttaatctc
     4981 ttttaacagt ctgttacatc gttgattttt gactcaaaat gattgtttct aacatgtatt
     5041 tatggtaaaa aagacaagat tagttctttg tacacatcga tttatacgct gaacaaaaag
     5101 aaatatataa actaagaaac ccattgtttt ttcgctccat cacatagagt ttactttaga
     5161 taaacagtga catttactaa tccataacgg aggaaatcaa atgaaaaaaa tctatttctt
     5221 atgtacgggc aattcatgcc gcagtcaaat agcagaaggc tacgcgcgta aatacttgtc
     5281 tccttcaatg tttgagataa gaagtgcggg gatcgagaca catggcttga atccaagagc
     5341 tataaaaatc atggcagaag acgggatcga tatttccaaa cagacgtctg atttgattga
     5401 tagagattac tttactcagg cagacttgat cattacactt tgtggtgatg ccaaagacaa
     5461 gtgccctgcc gttccaaaca atcacgaaca ccttcactgg gacctaaaag atcctgcaca
     5521 agctgtcggg agcgaagaag aaatattaac ggaattcaga aaagtcagag aaggaatcaa
     5581 aagaaaagtt ttagacttaa atcaacgtac atcgatcgta taaagaagta ttcaaaatga
     5641 tttttcaagc actaagaaac aatgttaaaa aaatagcttt ccatatttta caaaattgtg
     5701 ccttattgcc gacaaattgt ctcttgaaca ccgtttacca gtagtcaaga catcgtaagc
     5761 aactataaaa aattataaga aactcaaaat aaaaggatgt gccacaactt tggccacatc
     5821 ctcttttttt gtatttagtt ttctacttcc aaataaatag atagaattat tataaatata
     5881 aaggcagttt tttaatcgct acttattcat aactagataa caacgttaag atttctagcg
     5941 gatgattgga ttgcatcaac tgagccattt gttctggctg cttttctaga tagtgaataa
     6001 cagaaaatat ttttttgaac tctgcatgtt tcaaatcatt tttctttatt gctaatagca
     6061 gtacgatacg tatctgttcc ccgtgccata ggactggttc tttcattgta gcaatagaaa
     6121 tcatcgaatg cttcacatac agcggattac cgtgagggat tgttacaaat ggtgctactc
     6181 tagtactgga gtgactgtct ctttccaaga cagaatcgat gaattccggc tccacataat
     6241 gatgttccac taagacgttt cccataaagt ttaaaatatc ggctgggtct gtccgttcaa
     6301 gttgaggaaa gatcagaaat ggatgagtaa agcttgctag atcaaatgtt tttttcttct
     6361 tgataggcac atgctccagc atatattttg tcaaataggc tagatcagtt tctgtgatca
     6421 ttggtgagat ctcgattact ggaatgctta tatgtttaaa tggtaacgta gccaagataa
     6481 aatcaacaga gggaaaatct tgtctcttta attctggctt atattcaact agatccataa
     6541 tcgttaattc tggaagttct ctgttcagcc gtgcttcgag aaaagtagcc atagcttttg
     6601 aatattcact aactaaaacc gtttgatatc ttctactttt ctgatgtttg tgtcgctcga
     6661 tcgctgcttg gaaatggatc gttaaataag ctgtctcctc ttgtggaaca taaaaatttt
     6721 ccttttcaaa ataatcttct aaaattaact gtaaaactaa gaaaagttgt gtgtacgtac
     6781 taaacacatt tttcgtcaat ggattagaaa tctgcaaacc ataatggagt cgaaaataag
     6841 ttgaagataa atgagacctt aaattttgtt ttagtagctg atcttctttt aatgaatagc
     6901 ctagaaactg ttccacttgt tcgatcaaat gatcagcaag tgcgctagat tccttttgaa
     6961 aacgtgtggc tgcttgtgaa tttgtaaaaa gattggcgat acgcaaagcc agataattga
     7021 tttcatcagg agaaaattca attggataga tggtggctag ttttgctaat atttgactac
     7081 ttaacttttg tgcttgggtg tgttccacga cttcgttttc gtctttactt aatttcactt
     7141 tctggtactg tttcattcgc tcgagcataa agtagatgtg aatctccaca ctagccagtc
     7201 cattcggctg ttctaaatgg ttttcataaa aaacatcttg taatgtatgt tggataactt
     7261 ttaatgtatc tttagcgaag aattctttca acgacggact ttgtggatct tctttctgca
     7321 tatttcgtaa tgtcttaaca agcaactgtc ttttttcttg ttcatcgcct ttaagaaaag
     7381 ttcctagttt tgttttggat actagctgta aatggtgata ctcaaaaaag ctttgcaact
     7441 ctgtcaattc tttacgaatc gtacgttttc caacatatag tttttctgaa agttcttcaa
     7501 gactaaatcc gtcttttgct actagcagat ggaaaatcaa taactgacga agctgttttt
     7561 tatccatttc tgtttgagtc tcttcattaa gcgctcgttt cagttgttcc ctttcgtgag
     7621 gcagaccatt caacaagata cctgtacctt tttttctttc aaacattaaa ggcagatgtt
     7681 tgtctctcaa ccaagtttca atggtattca aatcgctatg aatcgttctt tctgacacat
     7741 ggaaatgctc tgctagcttt tttgttgtta tataattatt gttctcactt aattgataga
     7801 tcaaagattg ctgacgctga ttcataatgt gcagaaactc ccttctaaat aaaaaatcga
     7861 tcatgataac acatctatta tgtctcatca cggtcgattt ttcatcttat gttacatttc
     7921 gcttaataat tggtgaactg ttgttttatc tggcgcttcc atcagtgctt gacggaagtc
     7981 atcatccatc aatttacgtg atagacgttg cagtattttc aagtgcatat caccttggct
     8041 gttttcaggt acagcaatca taaagatcat atgtgcaggt tttccatcta aagaatgcca
     8101 atctacccca gattgacttc tggcaaatgc cactgttggt ttaagaacat aagctgattt
     8161 tccatgtgga atagcaatcc ccataccgat tcccgttgta ctttcatgtt ctctatcata
     8221 gatgacttct ttaaatctgg ttttatctgt gatcgccgct gttttttcta aacgttggac
     8281 taaatcttcg attactccgt cttttgttgt tgctgtttca cctaaaacga ttgcttcatc
     8341 tgacataata tccgtgatag aatcaaaagt ttcagttttc gtataaacat cacttgtttc
     8401 agtctctgat agatctgcta ctgtcacacc atttgctgca gcaaccgcag tttgttgtaa
     8461 tgtcggttta aggattctta acattagcac ggttacagca ataccagcaa tcactgctac
     8521 aaaaaacatg gctacgtgat ccactgctcc taaaaccgct acgatcggac cgccatgagc
     8581 tacatgatct gtcactcctg aaagcatcgc gatcacactt cctgtcattg ccccgattac
     8641 aatgcttgga atcacccgta gtggatctgt tgaagcaaac ggaatagcgc cttctgtaat
     8701 cccaaacagt cccattgcta aggacgcctt accagcttct cgttctgttt tcgtaaattt
     8761 ggtttttaaa atcaatgatg ccagccccaa tgctaatgga gggatacaga tagctacagc
     8821 aattggcccc ataatggaat aattgccttc tgcaatcaag ccagatccga acaaaaaggc
     8881 tactttattg aacggccccc ccatatcaaa agcaatcatc gctcctaaga tcatagctag
     8941 aacaatttca cttcctcctt gcatccctgc taaaaagtga gtcaatgaac taaatagaag
     9001 agctactggt tctccaataa catagataaa cacaaaacca actgccaatg atcctaaaat
     9061 tgggataaca ataattggca tgatcgattg taaagctttc ggtactcgca ctttcttgat
     9121 tcctaaagca atgaatcctg ctaaaaatcc agtgatgatc ccgcctaaga atcccgcatt
     9181 cgcatcacta ccatataatg ctccgtttgc cgctaacatc ccaccaacaa atccaggaac
     9241 aagtcctgga cgatctgcaa tactttgcgc aataaatgca gatagaattg gaaccatcaa
     9301 ccccatagcg atacttccga tactattcat cgttgcccaa atcgttcctt ctggtatctt
     9361 gatcccttca ggtgtagctg ttcctcctaa tgttaaagaa atcgcgatca ataaccctcc
     9421 tgtaactacg aaaggtacca tgtacgaaac gccattcatc aaactcttat agattttttt
     9481 acctaaattt tctgattcac ttttattttc ggaagaaata attgcctccg tacttttata
     9541 gactggcgct tttcctgcta aaatatctct caccaactca tctgcatggt ggattccttc
     9601 ttgcacacct acggttatca aaggtttacc gccgaatcgt gatttatcta ttttagtatc
     9661 tgctgcgatg attacaccag ctgcttcttc gatatctctt gcagtcaatt cattttcaat
     9721 accgatggaa ccatgtgttt cgacttttac ctcggcgcca actgctttcc ccgctttctc
     9781 taagttttct gctgccatgt atgtatgggc aataccaact ggacaggatg tgattgcaat
     9841 aatttttttc attttcctac cgcctttcta catttatagt atataaaaac ggtttctttt
     9901 tattaagcac aaaatgattc cgtaataact gaaattcttg gaataaatag aaatttcgct
     9961 aaaaaaattg atatacgatt tattatgttg aataaaataa tattggaaaa tcttacattt
    10021 ctgtacttct gcttacgtac atctaaatta ttatctacat taacaatttc cgtaattaat
    10081 tataaaaagc ttattccatt taatttcatt ctggaataag cttcttattt tctaatgagt
    10141 tagtgaataa ttttttttac catgcttaat agtatcttta cgtctcctat acgcgtatct
    10201 cctgtttctt tatctataat tgaactataa acatgaggaa tcactttttt tacacctgca
    10261 tcgattgcaa ttttcagaat ttcttcaaag tttgaaagat caattcctcc tgttggctct
    10321 aaatagaatt catattttgc gcaagcttcc gcaactgctt taaattcttc cctatgtgct
    10381 aagcctttca tcgggaaata cttgattgaa gtacctccca tgtcttgcag catttgaatg
    10441 gcagtttcaa taggcacttc agctgctggc gcttgtgaac ttaacggacc tgtagcaata
    10501 ttgacatagc cgatcttacc tgttggtgaa accaatccat tgatgactgt atcattttgg
    10561 cccaataaag cacgagaagt ccccacgcca gtaaatactt gattgacatg ttgtggttgt
    10621 aaaaccccag ataaacgcga aaccatctga ctttggtttg gatctccagc gcctaatcca
    10681 actgacaacg cattattggt tgcgtcttgg taccttttca tatcttcgat tgctgcttca
    10741 tcgctagcat aatttttcga tagcacacct agtacgacat gcccttccgc tgcttcatag
    10801 catgcttttg cattttcaac tgaatgagct aatacattta aacatacacg atttttataa
    10861 taatttggga taactgacaa tttaaaagcc tcctacatga tttctctcaa tctttttata
    10921 attttttcca tttcgttttg gctgacggaa cgaatgtcga actcaatgat tccgttattt
    10981 gcttgatatt ctcgggtgta gactgctgga tttcgtgctt ttaattcctg aatgacttct
    11041 ttcgcacttt tatcgccatt aatcgttaca cttgctcgat aaatatctct gccggcacca
    11101 tcttgaacta ttttcgctga aaggtcagga agttgattga ttgcgttaac aaaaggcgct
    11161 aatcgttgct tcatggagtc accggattcg ctcccatttt ttaaataatc ttctagtgcc
    11221 tgggtaaagc ctaaaatatt atctttaccg attttcattg ctcggccaat acctttagac
    11281 tgtagccgaa tccattgaat atacttcttt tttcctatga ccagtccggc actcggacct
    11341 tcgattgctt ttgccccaga gtaaatcact aagtcaacgc ccatttcgct atatttgaat
    11401 aaatcttctt ccgcagctgc atcaacgatc aatggcaaac gatgagcttt ggcaacttct
    11461 accatttctg ccaccgtcag catacttttc tgaactgcat gatgactttt aatatataga
    11521 agcgcagcag ttttttccgt aatcatcatg gaaacatgtt ccggtgtaca catatttgca
    11581 tagccagctt ctatgacttt tcctccacct tgagcaacca ttacttcaac cggtgtccca
    11641 taatctacat tatgtccctt aggtatgatg atttcccgtc tagcgatttt ttctgtatag
    11701 ggatgataga catggtacat gtcgccctgt ccgattaaag cagcaacaga ttgagcaatc
    11761 cctgctgaag cagaagaaac gatttgcgca tcttccactt tcagtaattg agaaagataa
    11821 gcgccggttt tgatggcaag atcactcatc tcaaaaaaat gctcgcctcc aaatcgttgt
    11881 gctgctagga cactttcaga taccttagaa acccctagga tcgtcatttt tcccgatgca
    11941 ttaatgactt cttttaaatc aaactttcca tagctaattg tcatatatga cgcctccaat
    12001 aattgtttta atcggtaaaa tttgttcttt agctatacgt gtaaatccgt tggaatcttt
    12061 caattccttt tcattttcaa caatctcaaa tatcgtcaaa tctgcatcat aattgacagc
    12121 taactggccc ttattcttca aatgaaaatt ccgtgcagga actgctgtaa ctttttcaat
    12181 gatttcttcc caagaatagc cgactaccct caatttttct aaagtagttg ctagatcaaa
    12241 aactgggcca tttgtacgat tgcggatgta gatatccgta ctaatagagg tagctttcat
    12301 tcctgctttt agtgcggttt ctgccacatg gaaattaaag ctatccgtcc catgtccaat
    12361 atcaaagacg acaccattat tgtaagcatc ccagacaaag tttttaattt gatctgtctt
    12421 tggatctaaa ataccgtttg gttttccatt aaaacaatgg gtcaatacat cgcctctttc
    12481 cattcttgac aatacttctt ctaactcagg aggtgcagaa ccgatatgaa ccatcaatgg
    12541 cagatcattg ttttctgtct ggattttttt agctaattct aatggcttaa tgccattttc
    12601 accaacaact gtcttactca ttctcgcttt aatcccgaca ataaattccg gcagttctgc
    12661 taacgcagta cttactagat cttcttgaat ctttgttaga tcagcaagtt catcttgttt
    12721 gacaatgccc catttagaga tattcattag cgcatacaca tttgtcttga ccgatttcgt
    12781 caattgataa aattcacgga tgttttctgc tcccgtagtt ccagcatcaa tgaccgtcgt
    12841 cacacctttt tcgaccccta tctgatcagg atagtcataa taaagattca tcttttcata
    12901 gcagtggaca tgatcatcga tccacccagc ggataaataa tactttccct ccaagtcaac
    12961 tacttcttgc gccggctgat ttatttctgc tgcaactgct ttaatcatgc ctttttctat
    13021 agcgacctct attttagatc catcaactaa ccttccattt ttaatcaaag tatcaaacat
    13081 ttaatgaccc tctttctatt aatttactgc cgggaagatt gctcctaaaa tagcacctcc
    13141 agcaattggt tttttccaag catagaatcc tagtgctcct aatgtagcgc caatgccgat
    13201 tggaatagat gctgcacacg cgcttaatat aattaatgga cccaaaaaac gtccagatga
    13261 atttcctgcc cccatcatga cgtcagcacc aaaagtggaa tttgattgat tgatcgtaaa
    13321 tttacgaatc aagacaatga tgccaccaac aatcaaacct agaaccaaac cagtcaataa
    13381 agagagtgca aaattatcga ttggtgccgc tattttcata cttaagagaa ttgctggtac
    13441 cccaattccg atcccagtta ggatcgaacc gcctaagtcc aagatcccaa ctaaagagcc
    13501 ttctaaaatt ctggcaaata agaaactagc accaaacgct gccgctgcgc cataggatcc
    13561 gccatccagc ccggctttta acatcgccac gatcgaaact tcattgaatg cacccacacc
    13621 atacatcaca tacatgtgtg ttcctgcaaa tacagcagcg gacataattc ctacgattag
    13681 cggaaaagac cagtctgcaa accaaaagct cttttgccgc tttgcttcta atacttttgc
    13741 ttgttcttct gttgtctcca tgattctcct cctatttcac actaaagatg ttgtgcaagt
    13801 tatcaagcca attaggcact tgaagctgga agctcttgat catttccata tcaaatccac
    13861 ggaaaaatcc actgaaaata aataataaaa caatagcgat catcatgctc ttcgtgacgc
    13921 gattccaacc gctctcatcg acgcctttac caatcaagat tcctagaaca acaccaggta
    13981 ccgcatttcc cataatcaat tgcgcgatac caccaaaaag agtgccccaa aaaccagttc
    14041 gtttccctgc gtctaaagct gccaaccaaa aaataactgg cattactgta ttgatcagaa
    14101 tcgtcgctgc tggtaccaaa acggaaaccg ccgtcacttg taacgcctct ggaattgctg
    14161 cagctgttgt gtttaagaag gctaccacca ctgccccgat aaccgctcca atgatcccca
    14221 tttttttcgg atcatgcatg gtctgggtga catctttgtt ttttgctaga agtgctgccg
    14281 ctgcccaatt gggaatgatt cgatgcgtca catcttgtgt gaatgcacca gcaccaactg
    14341 aagaagccca tgcattaaag aaaaagccta aaccaaatga aaaatgagaa gctggatcac
    14401 ccatgcaggc attcatttcg cctaatgttc taaaagctcc taaaccttgt gtattaggtg
    14461 catgaaacat tcgagctgcc ccgacagatg ctgcaaaacc aagtaatccc ccgataatga
    14521 ccgatttgac tagaatgatt acgaaatcca cgttattctt tcccccttat gattcaaaat
    14581 tagatttttt tagttacaaa tggaatggca atcccatttg gatcttcagt catttgctgg
    14641 ctgaacgata tgtctgccaa ttttatttct gttacagcca cagtaacaga gagttcgact
    14701 tcatagctaa tacgttgccg tgggaagaag aaaaatagaa agcgttctgt gtatttgttt
    14761 tcgaccgcct ttaaaatatc cacattgact ggttcgattc gcaataatac gtttgattgt
    14821 tccattattt tgttttgaat ttctgctaac gctgatgcaa aagcatgttg ctttgtttcc
    14881 cctttaccgg agacgataac tttttgctga attgtctttt gaacgctcat ttcatgtccc
    14941 tctttctatt ttccatgctt tttgataaat tcttctgtca gacgtttccc taattcctct
    15001 tgatccataa agccgaaacc tagaacagtt ttgccatcct tgatagctgt gatcccttct
    15061 tcaatcgaac gcataccatg tcgttctgga tagccatatt ttgttgctgc tgttaatgca
    15121 cctgcaccac cactaccaca aaaagagata cccatatctg catgctcttg ttgcatcaca
    15181 tcgcccaaac gcatatctgc ccccattcct ggtacgacaa ctgcttttcc accagctgct
    15241 tctacccctt tggccacatt ttgtccttta cccatacggt ctgcaataac tactttaatc
    15301 atgattgttt cctcctcgta aaatatcatt tgt
//
LOCUS       DAYYFD010000070        14704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000070 DAYYFD010000000
VERSION     DAYYFD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.070"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..2402
                     /locus_tag="ACHA25_002073"
     CDS             108..2402
                     /locus_tag="ACHA25_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8763358.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            2395..3060
                     /gene="pgmB"
                     /locus_tag="ACHA25_002074"
     CDS             2395..3060
                     /gene="pgmB"
                     /locus_tag="ACHA25_002074"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8763359.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            3053..4060
                     /locus_tag="ACHA25_002075"
     CDS             3053..4060
                     /locus_tag="ACHA25_002075"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8763360.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            4082..5101
                     /locus_tag="ACHA25_002076"
     CDS             4082..5101
                     /locus_tag="ACHA25_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8763361.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(5382..6749)
                     /gene="nhaC"
                     /locus_tag="ACHA25_002077"
     CDS             complement(5382..6749)
                     /gene="nhaC"
                     /locus_tag="ACHA25_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8763362.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(6858..8279)
                     /gene="tyrP"
                     /locus_tag="ACHA25_002078"
     CDS             complement(6858..8279)
                     /gene="tyrP"
                     /locus_tag="ACHA25_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8763363.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(8522..10399)
                     /gene="tdc"
                     /locus_tag="ACHA25_002079"
     CDS             complement(8522..10399)
                     /gene="tdc"
                     /locus_tag="ACHA25_002079"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8763364.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(10690..11946)
                     /gene="tyrS"
                     /locus_tag="ACHA25_002080"
     CDS             complement(10690..11946)
                     /gene="tyrS"
                     /locus_tag="ACHA25_002080"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8763365.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(12058..12263)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12526..13827
                     /locus_tag="ACHA25_002081"
                     /pseudo
     CDS             12526..13827
                     /locus_tag="ACHA25_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(13896..14495)
                     /locus_tag="ACHA25_002082"
     CDS             complement(13896..14495)
                     /locus_tag="ACHA25_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8763366.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         4580 a         3017 c         2722 g         4385 t
ORIGIN      
        1 ttattttttt aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg
       61 gttgcgttta ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca
      121 aacgactatt tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc
      181 ttcgactgca agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact
      241 ttgaagaaac ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc
      301 ccgataagac acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga
      361 tcaacgcctt gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg
      421 cttctttgga accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc
      481 gtacattcac aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga
      541 gcattgtcaa aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg
      601 gaacagtcaa actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg
      661 atgaacattt ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga
      721 ctaaaactat tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc
      781 atttcgttaa tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg
      841 gttgcttgtc tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg
      901 ttcttactag tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa
      961 cgaaccagac aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac
     1021 gatggaagct tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat
     1081 tcaatttgtt ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc
     1141 ccaaaggatt cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg
     1201 ctgtccctct ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc
     1261 ggcataatca actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc
     1321 tttatcctat ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg
     1381 aggagattca ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg
     1441 gcgatacgga atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct
     1501 gggcagaccg tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg
     1561 gcccaaacga atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt
     1621 gggtcttgcg ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta
     1681 tcagcgaaga agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg
     1741 acgaagaatt aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg
     1801 tatccgatat tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc
     1861 ttagatcttg cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata
     1921 cttttacaaa agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg
     1981 agtcttccct ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca
     2041 aagctgtaga aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca
     2101 cagaagacgg cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat
     2161 ttgctcaaat gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt
     2221 ggaaagctta ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag
     2281 atgaacaagt tactttcgag ttattagaag gttctcctct agaaatttac gtctatgaac
     2341 agtcttattc tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt
     2401 aaaggggtat tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt
     2461 gcttggcgaa agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag
     2521 ctaaaaggcg tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa
     2581 cactcttttt caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta
     2641 gagatgatcc agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat
     2701 tctttaaaag aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc
     2761 ctacttgaaa aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca
     2821 acaaacggaa aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa
     2881 gcagaagaat gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt
     2941 ggcgctcaac caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc
     3001 cctgatacga cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa
     3061 tccagagatc tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa
     3121 cgactactta gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc
     3181 agacaaagaa ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct
     3241 aacagacaaa agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc
     3301 ttcttggggt gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac
     3361 gaaaggctgg tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg
     3421 tgtggtcttt tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat
     3481 cacttaccgt ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga
     3541 aaccatctgc aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga
     3601 catctatgaa catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa
     3661 actgcctact ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc
     3721 aacactgcaa gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca
     3781 tcatcctagg ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat
     3841 gactatagca acttctaata aaagtatggt attattttct acgactggat tcgaagctga
     3901 tttctctgtc aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga
     3961 aatccctgat atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga
     4021 acggatcagt cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat
     4081 catggctatt accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc
     4141 tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat
     4201 tatggatgaa ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc
     4261 cagtgccatc ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt
     4321 ttatcttgag atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc
     4381 aatcgctact gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca
     4441 aaaacaagta gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta
     4501 tttataccaa aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga
     4561 aatcgtttat gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat
     4621 ccagaatgga cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt
     4681 cgaacgatat ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag
     4741 tgtagtacta gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga
     4801 agccacagca ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca
     4861 aatgtatggc tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt
     4921 ctcaacatta acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg
     4981 tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt
     5041 ggtcatccct catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta
     5101 aatagctgac tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag
     5161 aagatactac ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga
     5221 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
     5281 aaataagctg aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct
     5341 tggagttaaa cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga
     5401 cgattcaata aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg
     5461 aatggcaagt aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact
     5521 ccccacggta cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag
     5581 gtttttgata agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg
     5641 gacagatact gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg
     5701 actaaacgag aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc
     5761 agatatttga tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct
     5821 aacatactca tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt
     5881 gcgacataac cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg
     5941 ataaaagaaa gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt
     6001 tttttgattg cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa
     6061 aagctcgctt tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg
     6121 ttatgtccta atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa
     6181 cgcataccag cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc
     6241 gtatctgata aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta
     6301 gtcattgcat cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc
     6361 gaaatcgttg taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct
     6421 gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg
     6481 gttccagata aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga
     6541 atgatccctg gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt
     6601 ttaaatctgc cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag
     6661 agctggaatc cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata
     6721 ctttctttta aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc
     6781 tgccatgaaa aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg
     6841 ctgaaagact tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa
     6901 tctttcttga agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct
     6961 actaataatg catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata
     7021 gagaagatca gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg
     7081 taaggacgtt cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa
     7141 atgtacacga tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt
     7201 acgtcatcca ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga
     7261 acgacatttc tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg
     7321 aatccttcac gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat
     7381 tgtaaaagca caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag
     7441 cctaggattt gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca
     7501 tctggtacga catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc
     7561 aaagcgataa atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa
     7621 acagaactca tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa
     7681 tcaatatgtt ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt
     7741 gagactagtc cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg
     7801 atatataccc ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag
     7861 ttcaagatcg tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta
     7921 ttgccaagtt ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc
     7981 attaccatac ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg
     8041 ctgcccagtg ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa
     8101 agctcaccgg aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa
     8161 aaaatcaatg tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt
     8221 gccattgtca gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc
     8281 gttgacactc tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa
     8341 taaacgaaac aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt
     8401 tcttcaaaga caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca
     8461 gatccaaaca tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg
     8521 cttattttgc ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa
     8581 gagctgcttt gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg
     8641 tcattacgct ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga
     8701 atcctagttg gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag
     8761 tatgtgaagt taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat
     8821 atacgtcatg gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata
     8881 catagtctac catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc
     8941 ctactttaaa tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg
     9001 cacccattaa tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa
     9061 cgctggctgc tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg
     9121 ggatatctgc gcctttttca aatacatatg tcgcaaagta agagataacg tctctcatgc
     9181 ggatatcttt gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt
     9241 caatcgttac agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga
     9301 tataatcttt gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa
     9361 atggaatgaa gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag
     9421 catctacgtg taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga
     9481 ttttatcgat accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta
     9541 ggattggtgt tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca
     9601 tgcgatagtt gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt
     9661 cggcagcttt tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt
     9721 ctaaatgttt tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc
     9781 tgtctaacag attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc
     9841 cagcaactag ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg
     9901 cataccaaag tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat
     9961 ctttatagct cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg
    10021 caggagaaga ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta
    10081 gtaaagatgg catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc
    10141 aaggaacgga atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat
    10201 tcacagtgtg ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt
    10261 gaggcatgta gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt
    10321 tatagatttg gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt
    10381 tcgacaatga ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc
    10441 ctacattaca cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat
    10501 tttaatatgt atcaattatc cgaattgttc acacttttga aataattaaa aaaaacgagg
    10561 gaatttacaa atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt
    10621 taggatgggt ttcaaaattg gaatgttgta atagtatttc cctttttcaa ggtaaaaact
    10681 tactaaattc taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac
    10741 cacaaactta ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg
    10801 gtcaccgtta atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg
    10861 ttcaattttt gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac
    10921 gttcggcatt ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc
    10981 agagaacaat gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa
    11041 tcttgtaact tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac
    11101 ttttttcgct aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt
    11161 cactacatcg cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt
    11221 cttcggatca agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt
    11281 cagcatcaat gggatcgtta aaccgaacgc ttctgcttct ggtccttcta attttcggat
    11341 taggtctaat ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac
    11401 atcgtatgtt ttatgcagat gcaaaaagtc gatagattgc aggatttggt aagtaaactc
    11461 tgtaaatgag attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt
    11521 gatgttgaag tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca
    11581 gtcgtagttg ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt
    11641 ggaaagtgcg tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt
    11701 gcggccgctt gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg
    11761 gcctgccaat tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga
    11821 atcaccagtt ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg
    11881 aagtctttct tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat
    11941 attcatttta ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatatgt
    12001 aattaattat ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca
    12061 agggacgaaa tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg
    12121 aacatttaac gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt
    12181 aaagcatgta ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac
    12241 ttcgtttcga tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt
    12301 ctttgtcaac agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg
    12361 ctctaattcc gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt
    12421 tttctgcaat ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata
    12481 aggaatggga caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa
    12541 aaaattattt taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt
    12601 tttgtcatcc gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa
    12661 aaagcaaaag aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct
    12721 caaagctacg attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct
    12781 actgaaacac aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg
    12841 tctgatccta ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa
    12901 gccttccact ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc
    12961 aaccgttcga tcgaacaact ttatcaaaat gattttgttt tagttaattt gaatgatttg
    13021 atccaaaaag acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa
    13081 aaaccattgg ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt
    13141 tttcctagcc gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa
    13201 gataaagaag aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa
    13261 catcctgatt tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga
    13321 gttttaggtt atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag
    13381 attaaagaag ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc
    13441 catacatggg gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat
    13501 gaattgtggc agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca
    13561 tttggtgcag acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac
    13621 ttgaagagtt aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga
    13681 caattaggta cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa
    13741 tctgatttag acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat
    13801 gacaaagaag cacgcgaatc tgcttgattt tacgtcattt atgaaccaaa aaagccgaac
    13861 tgacatttta tcgtcagttt cggctttttt atgctttatt tcttcttcgg tgcgattccc
    13921 ttgatcagga cttctgtcca ttctgctgta tatggaagca gttttgcgta tatctccggc
    13981 ttagaaattc cttctaccat tgcttgaaaa tagaggatca agaactcatc cgaatatttc
    14041 aaatccactt ttccttcttt ccgccctcga tggaaaagat caagcatgat actgaaactt
    14101 tcttgcgtat acgcatccat cgcgtcagaa aattcttttt tttctttttt tgtatagtat
    14161 gtcatcaaat cgatataaaa ttgtttgttc actcgattga gataacggat tttgttttca
    14221 ctcatggcaa tcagtgtttc ttcaaatgga agattttccg caatcaacgc tttcgcttct
    14281 tgccctaaat cattcataaa tgcaaaaaat acttcataaa tcagcccttc cttgctgcca
    14341 aaatatttaa aaatcgtcgc tttacttacc tgagcgttct cagctacctt atccattgtc
    14401 actccagcgg ttcctatgtc tgtgttcatc aagtcaaaag ctgcttgtaa aatctctttc
    14461 tttttctctt gtgctctctt ttcaaaaccg ttcatttgta gagcctcccg taattttaaa
    14521 cttttataat atatatagtt caaatttatt ctgaggttta ttataactat ctattgagaa
    14581 agtatcaagc tagcaattcc ttgtttatga attcattgac agaaatttat aaaaagaata
    14641 ttatgtacta aaataataaa aaaagtttta taaaaaggta taaaaccttt ttattaaact
    14701 atct
//
LOCUS       DAYYFD010000071        14547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000071 DAYYFD010000000
VERSION     DAYYFD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.071"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..656
                     /locus_tag="ACHA25_002083"
     CDS             324..656
                     /locus_tag="ACHA25_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8763367.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            566..760
                     /locus_tag="ACHA25_002084"
     CDS             566..760
                     /locus_tag="ACHA25_002084"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8763368.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            799..945
                     /locus_tag="ACHA25_002085"
     CDS             799..945
                     /locus_tag="ACHA25_002085"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763369.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1383..2723
                     /locus_tag="ACHA25_002086"
                     /pseudo
     CDS             1383..2723
                     /locus_tag="ACHA25_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2964..4580)
                     /locus_tag="ACHA25_002087"
     CDS             complement(2964..4580)
                     /locus_tag="ACHA25_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8763370.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4698..4916
                     /locus_tag="ACHA25_002088"
     CDS             4698..4916
                     /locus_tag="ACHA25_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763371.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5111..5572
                     /locus_tag="ACHA25_002089"
     CDS             5111..5572
                     /locus_tag="ACHA25_002089"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8763372.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5765..6004)
                     /locus_tag="ACHA25_002090"
     CDS             complement(5765..6004)
                     /locus_tag="ACHA25_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763373.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6028..7551)
                     /locus_tag="ACHA25_002091"
     CDS             complement(6028..7551)
                     /locus_tag="ACHA25_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8763374.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7569..7691)
                     /locus_tag="ACHA25_002092"
     CDS             complement(7569..7691)
                     /locus_tag="ACHA25_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8763375.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7721..8704)
                     /locus_tag="ACHA25_002093"
     CDS             complement(7721..8704)
                     /locus_tag="ACHA25_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8763376.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8728..8877)
                     /gene="rpmG"
                     /locus_tag="ACHA25_002094"
     CDS             complement(8728..8877)
                     /gene="rpmG"
                     /locus_tag="ACHA25_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8763377.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8898..9275)
                     /locus_tag="ACHA25_002095"
     CDS             complement(8898..9275)
                     /locus_tag="ACHA25_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763378.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9307..9486)
                     /gene="rpmF"
                     /locus_tag="ACHA25_002096"
     CDS             complement(9307..9486)
                     /gene="rpmF"
                     /locus_tag="ACHA25_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8763379.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9501..9770)
                     /gene="rpsN"
                     /locus_tag="ACHA25_002097"
     CDS             complement(9501..9770)
                     /gene="rpsN"
                     /locus_tag="ACHA25_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8763380.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10293..12059)
                     /locus_tag="ACHA25_002098"
     CDS             complement(10293..12059)
                     /locus_tag="ACHA25_002098"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763381.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12019..13773)
                     /locus_tag="ACHA25_002099"
     CDS             complement(12019..13773)
                     /locus_tag="ACHA25_002099"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763382.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(13883..14452)
                     /locus_tag="ACHA25_002100"
     CDS             complement(13883..14452)
                     /locus_tag="ACHA25_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8763383.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         4641 a         2835 c         2216 g         4855 t
ORIGIN      
        1 tcacagtctc actcctaaaa ttactttatt tacgtatgaa gtgggcagga ggtcgaatcc
       61 agaactgggc tatggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
      121 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
      181 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
      241 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
      301 gttaccggaa ggaatgaact ctattgagaa aaacatatga tcgtaaattt aaactaaaga
      361 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
      421 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
      481 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
      541 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
      601 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
      661 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
      721 tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga atcgataatt tgaacagaac
      781 caaattgatg ctgtttgggt gatggatatt acttacattc catgtaatga cggtcgcctc
      841 tatttgtcca cctatattga cttagcaact cgtattctac gttactatat ggtagattca
      901 cacatgaaaa aagagattgt cgtccgtcca ttacaaattt attaaggaga attgcctacg
      961 gtaattcatt ccgatagagg tagccagcat cggtctttta tttatcaaga gctgttgaac
     1021 gaccaccaca tcacacattc agtgagccaa actgggactc ctgttgacaa tgcagtaatt
     1081 gaagcgtttc atcgatcaat caaatgagaa ttaatcgact ccaacaaata taaaagtaaa
     1141 atagagatga aggtgttaat ccaagattat ttagaagact actacccaaa caaaagaatc
     1201 catacaaaat ttatgatgac tccttgacaa tttgaagcga agaaaataag ggatctataa
     1261 tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta ataaacgcaa
     1321 acagacacct taaaatcctt tagaataaag tcgacgaaaa ccattcaaag gagtgtttca
     1381 agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta aatcttatct
     1441 ttcatgaaga tgcctttcgc aaagagcgaa tcaatgacga aacatgtttt gtttttgatg
     1501 gcacattgac ctatcaaccg gaggaatgct ttcatcgcca ctatcaaaat gaacaaacga
     1561 tcattaaata gggctggaaa aaagtatcca tcttattgaa tgatgtcagt aactacaaga
     1621 ccattctgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag acttttttag
     1681 cagaagattc agttagtgat cgtcgctgct ccattgctcg cagagtcaaa caagccattc
     1741 ttgagctatt aagcgaacct ctttctatgt ctttgatcgc acgtatgaag cacatttctc
     1801 cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccatttct ttgagccagc
     1861 tgttaccaga agtggtgtgt ttcgatgaat tcaaatcagt gaaaaatgtc tcgggagcca
     1921 tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg gaaaatcggc
     1981 aactcaatcc tttacgcgac tatttcttgc gctaccctat aaaagtccgt gaacaagtgc
     2041 gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag tgttttccga
     2101 atgcccgaat cgtcgccgat cgctttcata ttagccaaca cattggtcgg gcttttacca
     2161 accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg aagcacttaa
     2221 agaaatactg gaaactactt caaaagaatg cgtgggaatt gaaaggtcag catcgctact
     2281 ggcgtccgtc atttcgagat catctgacag aagcagagat agtcgatcgg ctattatctt
     2341 atgatgattc cttaaagcga ggctacgaag tctaccagga ttttctttcc gttatcagaa
     2401 gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag ttgccagagc
     2461 actaccaacc agtgttcacg accttcaaga aataccaaac agaaatcaaa cgagcgttgc
     2521 gcgttcccta ttccaacggc cctatagaat gtttgaataa ccatatcaaa gtactaaaac
     2581 gaattgccta tggctataga aactttcaaa actaccgaga acgaatcttt ttatatcgag
     2641 gaaaatattt taagaaaact aagaatacta cccaactgac taaagccaga acaacaacac
     2701 ggctagacaa gatagctgtc tagccgtgtg agtatctcga ttattctatt gaatttcttc
     2761 accaacactc attgacgaag agccaaaata agtaatctaa ccgactgtta actaaaccat
     2821 tgcaggtccc gttaaagata cttgtgcctg ttgtcaccgc ttctttttct tgtccacaag
     2881 cgcttaacac acttaataaa gcccctgcta ttattaataa taatatcttt ttcttattaa
     2941 aaacctcctc atctatttcc agattagtaa atgtagtaat ctgtgctttc ttttttctca
     3001 atactaataa ctaacttatg gggtaaacaa atactcgttt gaccgggttt tgagatccat
     3061 ccagttctaa ccgcgatttg atcaggacta ttatcttctt taactcgaat tcttccatct
     3121 ttaatctcaa caatattata ttgtcccttc gcaggatgat aattcacgag tttatgattg
     3181 atcttatcca aatcgaagcg atccatttcc ttgccatcaa ttcgaacgat ggcatatttg
     3241 atagcatctt cagttgtttc attctccttt tctatagaga atacaaataa gggaataaaa
     3301 gaggtaataa tcaaaatgcc aataattatg acatcgccta tcttgacacc agcttttctt
     3361 aaaaatttca ttaacgatat ctggctaaac atattagact ttttccatgg aaatggtgaa
     3421 atttttcgga aattatctgc attaagagaa tcagttaaac aaccctcaag ttgttcctta
     3481 atacattgtt gatccatctt cataccaata aaaactagtt tattaatgcg ttcaccaaac
     3541 aacggatgcc aggcttcctt caattcggga tttttttgta taatcatttg acactcaatt
     3601 ttaggtaaac tagctaccca ataatttaat gattcaatac ttttatagct acccgatgtt
     3661 tcaaagatcg tcgaaaaatc actatgttgg gcaaaccaaa gaaacccttt tgctcttgta
     3721 atattgccag gaaaattttt atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac
     3781 cgatttcgat agataaaact tgagatacca tattcttctg tttcaggaat atgattttga
     3841 tagccaagct ctaactcttt gaaccagccc actgaatagg aagtctcatc tatattaaaa
     3901 agatttgtat ttagaatttt cgataagtct acttcacctt taattgtcct aattattttg
     3961 gcattggact gtaacttagc aattagaccg ttaattttgt aaatcgtctt ggttgacact
     4021 aagtcactat tatttaaaag taatatgtta caacactcga attgatcaat taacaagcca
     4081 cgaatatcct gttcttcttc aactacatta ccttcagctt ttctttcctg aatcttttca
     4141 cccgtttcga aattatccca aaaacgtttt acatccacta ctataataat ggcaccaatc
     4201 tctaatcgtt gggtcaaatc aatgtctaaa gggtttttag ctaatataat tgcttgaata
     4261 attggaattg gtttgctgat tcctgaagct tcaataacaa tttgattcaa gtcagaaaat
     4321 tgcactaatt gatctagttc aacaatcaaa tcttcgcgta atgtacaaca aatgcatcca
     4381 ttcggcatcg aaacaagttt ttctccagtt cttttaaaac ctttcttgtc gactaatacc
     4441 ccatcaacat ttacttcccc tatgtcattt acaatgacag caattttctt tccgtgatca
     4501 ccgtacagaa tatgatttag aagtatcgtt tttcctgaac caagatagcc agttaagatt
     4561 gtaataggta tttttttcat agggaacctc tttctatacc ttgaatttac actttaagtg
     4621 caacacataa aaatgagtca taatcgatat ccaactagta taagtttgtc gagaacactc
     4681 ctagaaggaa aataattatg agccagtatc gtcatcttac tatactcgaa cgagaaagaa
     4741 tctttctgct tcacgaagaa ggattttcta ttagtataat tggcaacgac attcagcata
     4801 gcccgtttac tattttacat gaactgagaa gaaacaagaa aaaaactatt caccttccaa
     4861 agcggaaaaa aattatgctt atcgaagaaa aaaatgcgga cgttatcaca ttctaaatga
     4921 tccgtgagtt tggtcaattg ttcgtcattt atttgtcgat tttcagtggt cgccagagga
     4981 aatctcgaat cgtttgaagc atgaacatat ggacttttca attagtttta tcacgatcta
     5041 acgtgcaatt tgcaatgatc ttttcgatac agaacccctc tcacacggca ataaaggcct
     5101 gatccgttca ttgcaccatc gaggaaaaac aagacataca aaaaatcatg tcgagtctcg
     5161 tggaaagatt tgccttactc attcgatcca tggccgtcca aaatccgcag atgatagaag
     5221 tgattttggt cattggaaag ccgatattgt agctggcaaa actggtggta cttgtttagt
     5281 gactttgaca gacagaaaat catgcttcct tttagcagga aagaaggtcc ccagaaagaa
     5341 agccgcttat gtgagcgaga aaatgattga acttttaagc agtattccaa atgaggcggt
     5401 caaaacgatc actccagata gaggaaaaga attctccaat catttcgcag ttacagaagc
     5461 attgaataat atgccctttt attttccaga tcctcatgcc cttggcaaca atgaacaaat
     5521 taaaatacga atagactcat tcgagaatac ttgccaaaat cgaaagagat agctacggta
     5581 gataactcaa cgataaatca gatgattgaa aaactgaata acagacctag aaagtgttta
     5641 gattggagaa caccttatga agtcttcttt gataaagtgt tgcgcttgat ttgacaattc
     5701 aagacataat aaaaagcaga tcaaatccaa ataagggaaa aatgatctac tctggtatta
     5761 tcaattaatt ggccaagcta ttatgccgtt ttgctaaacg tctacttctt ccagtattga
     5821 ccttgataaa acaacaatca ggatcccgtt ttaatactct atgaactgtg tcaatactaa
     5881 catgtaaagt tgcagcaata tcagctaatg tactcccatt taatctcatt tgctttgcaa
     5941 atcgataacg ccgataagca tcaatattct tggaatccat agacaataat acttttctta
     6001 ccatgtattt cctccaaaaa ttgaatttta atgagccatc atttcttgag caatttcatg
     6061 tttagacaat gaagctggat agaaagtagg ccaattatgc aattcattat agagtttttc
     6121 ttgactttct ccaccaaaga aaatatggaa atgctctgcc ttacttggtg caattccatg
     6181 atcactaaat tgaatatatt taaaacgacc agcacctgca tcatcagttt caaaattaaa
     6241 acgaacacca cggtttcctt tttcataatt caaaattttg tatccaacat atttgtatgt
     6301 gtattgatga tgttcaccgt tgactacaaa atcaatggtg ttgtctttaa tgttgatagt
     6361 atcaatatct gtcttatagc cagtagtata ataatctttg tactcggcag ctgtcatgtc
     6421 tttattgatt tttgctttgt aatcgaaaac ttcatccaaa attccatctt ttaacaacgg
     6481 ataaacagat tgccattcgc ctgtataatc actcaattcg cgatccttca catcggcatc
     6541 tttaaagtac ccattagcaa ctgtcttttc atccttcgct tctttttctg gaactacctc
     6601 ttcaccagca accatagttg ttttttctaa cgcttttaga ttgtcttcca ttactgaaac
     6661 ataatcttca ccattatcca tttgttcttt tgtaagactt tcaagaggat tcagtacttc
     6721 caattttatc ccagcttcgt tggccaaagt ttttgcaatt ttatcattag cattcttttc
     6781 aaagtaaata taattgatct tatttttttt aacatactct tttaattcag ctaatcgacc
     6841 agaagatggc tcttcttcag gacttagtcc agcaatagga acttgtttca atccataatc
     6901 caaagctaaa tatccaaagg ctgcgtgttg cgtcacaaaa cttttttgtt tcgcttcttt
     6961 aagactagtg gtatagtcag catccaaccg tttcagttta gtcaaatact tttcagcatt
     7021 tgtctcaaat acttttgctt ttttcggata caatttaact aactgatcct tgatatttga
     7081 aacttcctta attgccattt tgggtgaaac ccacgtatga ggatctagtt catgatggtg
     7141 atcttcttct ccgtgatcat gatcttcttc ttcactacct ggcaataaaa tcatgtcttt
     7201 tgttccttct actacattag gcccagcttt cttccaactt tctttagctt ttggtaccca
     7261 tgattccatg ttttcgttat gatatacaaa aacatcagca tcatgagctt ttgccatatc
     7321 cttagctgag ggctcatagt catgaggttc tgatcccgct ggaaccatta aatccacgtt
     7381 cgcttcatcg ccgacgatgt ttttggtgaa atcatacatt ggatagaatg ttgttacaat
     7441 ttctaatttt ccatctttat cattcgatac ttttgcatca ttagtgctgg agcaagctcc
     7501 caataatcct agagctgcta tcccaataag tcctacactg attttcttca ttctttttcc
     7561 ttccttaatt atgctaattt cttcatttta tttcgtttat gttctttgat aatttttaat
     7621 gccctttttc tagttttaat attcgggctt ttaagtcgtc gatatgcact agatatatcc
     7681 gttttctcca ttagaaatta cccccaatca ctacttatca ttatttgctt tccgccagta
     7741 atttatcaaa actctgtttg atcctttctt catccaacat ttttccgatt agaataatag
     7801 tggtttggtc tttttgtcga gtggtgtatt gaaaattaac ctgttcgttt acctcttgca
     7861 tggcaactat aaaatcctgc tcttgtaaag caatcaatcc tttaaaacga tacaggctcc
     7921 cttggttggt taaaatcagc cagtcaatcc attggagtaa aaaatctttt tttatgtctt
     7981 gatctgaagt taagagaatt gatttaaact catgtacttg atggtgttga tgatagtatt
     8041 tgccattata gtgattttct gatgaatcac tttcaaaaac taagggatga ttaaattttt
     8101 tcaaattata aaaatccttt ttttgaatcg tttcactatg tgtgaacatt cgaatatcgg
     8161 ataaagggtt aatacttttg atttctctct gtaatttttt tagtgcagat aaattcaagt
     8221 tttgttttct agacacaaaa atgcgatctg ccatgacaag ttgcttaagg gcttgtggtt
     8281 ctctttcaat aagttcaaaa ctgtcactat caataacagt caataaacta tcgatatata
     8341 ctcttcctct tagttgaggc gctgttaaaa gtgtttgaat gattggttgg ggatctgcta
     8401 tacctgtagt ttcaatgatg atttgtgaaa ttggttgttg atgttcttct attgtttcca
     8461 gtaaagaaat caatattgct aataaatcac tgcgaagact gcaacacata catccattat
     8521 ttagttgtaa cacccgttcc tcgctatgaa ctaataattt gtgatcaatc ccaacttcac
     8581 caaactcatt ttcaattata ataatttctt ccctggggta gggtgagact tgcaaggctt
     8641 gattgattag cgttgttttt ccagccccta aaaacccaga aacgattgta actggtatgc
     8701 ccattttgtc acctttttac cacttagcta tttcacttct ttaaaaattg cccgtctgcg
     8761 caattttggc gaatactttt ttaattctaa ccgttcaggg ttattccgtt tatttttgct
     8821 ggtgagatac agacgttctc cagtttcgac acattccaaa ataatatttt gacgcataaa
     8881 tcttcctcct tcaatactta tcgatcttca agatatgctt gaatttgttg tgcatcttca
     8941 aaagttaact cgccagattc caattgtgaa actaaatttt ctttcatatc aacaggtatc
     9001 tcccaataac catcgataca ttgaaataac tcctcgtcat actcttcaaa aattggatag
     9061 attcttaaaa aatcatcaaa tgataagcct tggtcctcat agtaatcttt gactaatcca
     9121 ttttcaattg cctgttgggt cggtaaaccc gtttcagtaa taattcctct ctctaccatc
     9181 gaacgataaa aagtgcattt atctagcgat tgctgggtcg tttcaagata atactttaat
     9241 ccttcttcaa ctaaaggtgg gtaaataatt tccattttga ttccctccta aaagttatat
     9301 attaaactat tttgaagctc ctttaattac tttctttcca ttatagtacc cttttaaaga
     9361 tacatgatgg ctacgtctat aatcaccatt cgcttcgtca aatgaaattt ctggttttac
     9421 tacagtttga tgagttcttc ttaaccgttt cttcgcctta gatgtttttc ttgctggtac
     9481 tgccatgata tttcctcctt ttaccaactt gcttttttta ctccaggaat caacccttga
     9541 tgggcaagtt ctctaaactt aatacgggac ataccgaact ttcgcatata ccctcttgga
     9601 cgaccatccg tctgatcacg aagcctcaga cgattcggat ttgaatctct tggcaattta
     9661 gcgagagcgg tgcgatctcc tgcggctttt aattcctgac gtttatctgc atacttttca
     9721 atcatcgcca tttgtttctt tgctttagca attttcgact tttttgccat atacctttat
     9781 actcctttta ttttgattta gacacctcaa atcgtaatcg ttacgttttg acactgatac
     9841 tttaccaaca agagttgctc ttgtcaacaa taaataacat gaaataaaac tcatatgaat
     9901 agataactat ttgaaaacga atttatcggt acatagagat aatcaaatca tttaaaagaa
     9961 tatgcagttt aggctctgaa aaataagaat tcacttttat tagtccaatc aaacacaaat
    10021 accgttttaa gtagcttttc ctctacttaa aacggcaata tgaaagaaac tataactttt
    10081 tattcaatct tttatgttta actggtttat cgccaatttt ttaaaacttt gattattcct
    10141 tatattataa gaatagacat taagtgaact aattcaatca tactgattct ttcgtgtatt
    10201 caaaattaga tgctgtttaa taaaattatt ctttttgaaa aagaattata actatatccc
    10261 aaaaattgta ggttaattta aagcctattt catcatcgtg attgagcttc tttttcatag
    10321 acgaatttct cagatagttt agttttcttt atgagatttt tgtatatttc tgaccttcga
    10381 tacaactcat tatgagtacc ttgatcaact agttttcctt gatccatcac tacaatttta
    10441 tttacctttt caatagactt caagcgatgc gatataatta gtactgtctt atcttgtatt
    10501 aaacgattta aactttcctg aatacgtttt tcattttccg catctaaact ggcagtaatt
    10561 tcatctaaaa ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga taggcgttgt
    10621 cgctctcctc cagaaagtga acttccgttt tctccgacca atgtatgata accgtcaggt
    10681 agtttttcaa taaagtcttc gcagttagct aaacgagctg cttttttcac ttcctcatct
    10741 gacgcttgac tatttccaat acgtatattt tccaaaatac ttgtattaaa caaagttacc
    10801 tcttgaaaaa caatagcgat tttgctaaac aaagaagctg gagatacacg tttaatatca
    10861 tacccatcaa ttaaaataca gccttcatca taatcataca gtctagacac taatttcaag
    10921 atactagttt tcccggaacc actagcacct actaacgcag tcacttctcc ttgtttagcc
    10981 gtaaaagaga tatgatctaa aattggggta ttgttatcat atgagaaaga gacatctcgc
    11041 aactcaatgt caaaagattt cagaggacta tctgatccct cttggatact ggtttccttc
    11101 atagctctaa ttcgctgtat tttaggagct aaataaaata tttcaagcac tgcttctttc
    11161 atagaatcga aggaatcctt tatttttatt gcggctagta agtaaccaac gacgtagagt
    11221 atagtcactt cacccgttaa cagtaagtga acacctacta gaatgactac tgctaaacta
    11281 acatatgaga aaatagagga taaggccatg actgaaaaag tacttaattc tacctttaaa
    11341 tgaatccttt cactctcttc catttttttg aaaagtctgt cttgaacttt tttagataga
    11401 ttaaagctat taatctcttg ctgcaattct atagtttctt gaaattcttc cgagttctct
    11461 ctcaaagtat cgtaatattt agtattggct ttcgtttggg atttctttga taacaagatt
    11521 aagacaaaac taaataacgt tggcaaaata acagctaatc ccattttgac attaccaacc
    11581 aaaagcatca ctgaaataaa agggaaaaac agagccatac cgccggcttt aggtattgca
    11641 tgactcatcg catgctcaat accttcaacg tcagacatga tagtttgaga taaatctgat
    11701 aaattatgtt ttgaaaaata ggataatggt aaatttgaca aatcgtctgc aatttgcact
    11761 cttaaatgcg cactttcttt ataagtacta ttatataagt tttcatactc cctatctaac
    11821 aaccaataca tgaaaagcaa agtaacaaac gatacggcaa aataaagcca acggggcttt
    11881 gtattttcta aaactaattc gtctatcagc atcattataa tgaatgctgg taccatatta
    11941 atgcagtata caaaaaaact agaaatagaa gctttagtta aagcttctgc gccctgatca
    12001 cttaaagcaa atttgttttt ataaaaactt ttcattgtca agcctccaat catttgcatt
    12061 ctcataatta gtgaccaatt gtttatacac ttgatgttta gccattaatt cattatgatt
    12121 tccacgttct actattttcc cttctgacaa gacaagaatt tcatctacag atttaatgct
    12181 ggataatcga tgagctatca taataactgt tttttctttc atcagatgtt gaaaagcctt
    12241 ttgtagttca tactcattat ctggatcaat agatgcactg gcttcatcca taataacaat
    12301 ctttgcatct tttaaaattg ctctggcaat agctatacgt tgtttttctc ctcctgacag
    12361 ataaactcct ttactaccaa ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga
    12421 ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt gctggcgctt gaggatttgc
    12481 tatagaaacg ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa agacaaaagc
    12541 aattgtgtta ataattgtgt cttttgaata gtgatttaaa ggcttaccac caattttaat
    12601 ctgtccctta tccacattgt agaaacctga aattagttta gctatcgtac tctttccgct
    12661 tcctgatttt cctactaatg cataaattct tttttgttct aaaactaagt tgaaattttc
    12721 taaaacacac tcttctttgt atgaaaagct gacattttca aatgcaatgt cacaattttt
    12781 aatctgtgtc tcgttcccat attttagttg gtcttgcttc atttctaaat aaagggagtc
    12841 tagtgtttcg actgcatatc ttgcttcaaa caagtgcata cttacataca ttattctcat
    12901 gaaagaaaca aaaataattc cacttaaaaa aaacaacatc aacaagtcaa cagctaatat
    12961 cttgggagtg gtaagagaag ttataaaaaa ggttatagga actatcaaaa tgcaaactat
    13021 gccaaaaaat agccattgaa aaagaacaaa aggtgttttc ccactcaaag aatacttata
    13081 tgcaaactta gcataatcgt taattgcttt attcaaggct ttaaatgatg ttactttagc
    13141 accaaatatc tttataactt gaattccgcg gatatattct accgtttcag agcttaacac
    13201 atctaacgat tcttgatatt tttgaataaa atttgtattc tctgtcattt tctttaacaa
    13261 gaatacacca attactaaca tggcgattag aaccaatcca attttaaagt taatgaaaaa
    13321 gcctaaggca actgataaaa caggtgttac tatggcttga gcactatctg gaatcaagtg
    13381 agcaattgcc atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga
    13441 agaatttaaa tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct
    13501 caaattagtc tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa
    13561 taaagctcct aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc
    13621 atctcctaca ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt
    13681 taatacgaca gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg
    13741 gacataacta aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt
    13801 ctcttattcg tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa
    13861 cagataaaag gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca
    13921 atacaccaat catagataaa cttgctacaa ctaacactga aatgattccc catataccta
    13981 ccgcatagtg gatcattgca tccattgttg cagcagattg tcctttatct atccaatctt
    14041 gacgaaaacg atctgtaaag aaccatatag gaatccactg tccggcagca ctgcctaatt
    14101 gcattagagc ccaagacgca acttgaggaa ttgctcgatc atatccaaat acagatagaa
    14161 taatatcagc taagatcgcc atactaatcg aactgataaa ccaaggtaag tacgccccgc
    14221 ccattaacgt aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag
    14281 ggtagttata gctaatataa acaaatatga ttccagataa taatccaaaa atcccggggg
    14341 atatgctgtg accaaacgcc ccaccaatac tactaaccat tattgaaaaa aagtaaatta
    14401 ctagggaaat aactgtagct aaaccaataa aaatataatc ttttgttttc atatatattc
    14461 tcctcaattc tatttcgtta tgtcattaaa aatctgttct atcaaagttg aatcttctac
    14521 tctaagtttt atatcttttt ttattcc
//
LOCUS       DAYYFD010000072        13893 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000072 DAYYFD010000000
VERSION     DAYYFD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.072"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..2333
                     /gene="acm"
                     /locus_tag="ACHA25_002101"
     CDS             168..2333
                     /gene="acm"
                     /locus_tag="ACHA25_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8763384.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2605..3090)
                     /locus_tag="ACHA25_002102"
     CDS             complement(2605..3090)
                     /locus_tag="ACHA25_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8763385.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3180..3525
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3570..4211
                     /locus_tag="ACHA25_002103"
     CDS             3570..4211
                     /locus_tag="ACHA25_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8763386.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4230..4865
                     /locus_tag="ACHA25_002104"
     CDS             4230..4865
                     /locus_tag="ACHA25_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8763387.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4883..5698
                     /locus_tag="ACHA25_002105"
     CDS             4883..5698
                     /locus_tag="ACHA25_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8763388.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6017..7453
                     /locus_tag="ACHA25_002106"
     CDS             6017..7453
                     /locus_tag="ACHA25_002106"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8763389.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7642..10074
                     /locus_tag="ACHA25_002107"
     CDS             7642..10074
                     /locus_tag="ACHA25_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8763390.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10304..11371
                     /gene="rlmN"
                     /locus_tag="ACHA25_002108"
     CDS             10304..11371
                     /gene="rlmN"
                     /locus_tag="ACHA25_002108"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8763391.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRSEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11361..11567
                     /locus_tag="ACHA25_002109"
     CDS             11361..11567
                     /locus_tag="ACHA25_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763392.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11564..12667)
                     /locus_tag="ACHA25_002110"
     CDS             complement(11564..12667)
                     /locus_tag="ACHA25_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8763393.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13219..13767
                     /gene="aac(6')"
                     /locus_tag="ACHA25_002111"
     CDS             13219..13767
                     /gene="aac(6')"
                     /locus_tag="ACHA25_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8763394.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4758 a         2299 c         2939 g         3897 t
ORIGIN      
        1 tgtaaaaaca gttttttggc atatttattt tttataaata tttgtaaaat aactatcaat
       61 tttttagtta aaagattgga aatgagtggg ggaggaattg aagaaaggat ttttgagaga
      121 tgatatagta ggttttattt ttagttaaaa taggggagat agaaaaaatg aaaaagtgta
      181 gaaatattat tttatcaatg ttatttatta ttactaatat aacatcactg attcctgttc
      241 atgtctatgc ggatgcaggc agagatatca gcagtaatgt cacttcgttg accgtggacc
      301 caacgaatat tacagatgga ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac
      361 aaaatatcca accaggtgat tatctttgga tcaattggcc aagtgaagga aatattcgcg
      421 gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa taaaaatgtt ggaactttaa
      481 cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa tattaaaaac ctagactcag
      541 tagaaggttg ggggcaattt gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa
      601 atacgggacc attcaccgtg acgagcggtg ataaaacagc tactgtaaat gtgacaaagc
      661 cagcttctgg ttcttcttct agcgtttttt attataaaac aggagacatg ctacctgaag
      721 atactaagca cattcgatgg tttttgaata ttaacaataa cggcacttat gttgaacaac
      781 cagttaaaat cagtgatgaa atccaaagtg gacaaagatt agatccaagt acgtttgaaa
      841 ttaatcaaat acacttaggt gaacagaagg tttatagagg agaagaggga atccaacaat
      901 ttttacaaga tttccccagc gctacattta atttttcagt aactgataat tacattgaaa
      961 taactatacc aaaaaacttt gtcaatctta ggaaaataat ggtgagttat aagacgatca
     1021 tagaaaaccc agaacaaata aattttgaga atcattcgga agcttggttt aaagaattta
     1081 ataaacctgc tgttgatgga gaaagtttca atcatacagt taaaaatatt tcagcttctg
     1141 gtggagtcaa cggtacagtt agaggcgaat tgaagatttt caaatatatc aatgacacag
     1201 agattggtat cccaaatgtg actttcgaat taagacgagc agatgaacag cctattcaag
     1261 gacagtctag tatcctgttg acttcgaatg aacagggaga agctagtatc aaaggcctgc
     1321 aagtaggaga ctatgttgta aaagaaaaag aagcacccaa ttggattgat tttgatccac
     1381 taagttcaaa tgaactcaaa ttctccataa acgaaaatga tacagaggga gtcagtttac
     1441 ctatttataa caagaaaaag gttacgaaca tcacagcaac taaaatttgg aacggtggaa
     1501 ccactccaag accgagtatt tattttaaat tattcagagc aagtacaaac aattgggaac
     1561 cagttcctga tgcagaaaca aaacgattag acaatggaat aacttcagtg acttgggaag
     1621 atatccaaca gtatgatgat agtggaaacg aatatacgtt taaagtgcaa gaagtagatc
     1681 aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta
     1741 caaatgagaa taaagaagtc atttcagtct caggacaaaa aacatgggaa gacaatgaga
     1801 accaagacgg caaacgaccg acgacgatca cagtaaatct attagccgac ggtcaaccga
     1861 tccaacacaa agaagtaagt gaaaaagatg attggcgcta tcgtttcaca aatctgccaa
     1921 aatacagaga cgggcaagaa atcatctata ctgtaacgga agacaacgtg cccgaataca
     1981 gtacgacgat tgacgggtat aacatcaaaa acagttatac accggaaaaa acaaatatat
     2041 ctataccagg tagccatata acaccgtgga aaaattttcc agagaagaca gagagagaga
     2101 aaattagaaa tctattctct caaaaattgc aaatatttgc tcatacagga gaatccagcg
     2161 aacaaagaag caattatatt agtaagagag ctgtacctaa acagataaca aataaatcaa
     2221 aaagcattct tcctaaaaca agtagtaaaa aatctagttt tgctgtaatc attggtttat
     2281 tgatagtaac tatctgtgct gggatattct tacaaaaata taaaaaagtt tagttagaat
     2341 ataatatttt ttgttaaata tgactgaaat caaaagaata aaattccgat ttataatcga
     2401 aaaaagaagc atttttatat atgcttcttt tttcgtagaa tcaataaata tggatttggc
     2461 taactatgga agaatactcg tattagtttt cagcgttcat actagttgtt acattgtttg
     2521 attggccaaa agaatttctg atatattttg tgctaaataa agaaaaagac gcttgaattg
     2581 ggaaataaga aaactactct atcattacaa attttcataa actggcagat gttctaagta
     2641 aacaagtatc ttttcaaagt cggaaaaatc cataatcaac gtagtggtat tgatatttgg
     2701 atgaaagcct acggtatctt gacgatcaat agcagcatcg atcacgactt ggattttttt
     2761 ctctttgtcg tgcatcaatg ctaatggagt cactgtacct gctggaacgc ctaggaggtc
     2821 aggaagctgt tcttcagaaa caaaagataa gcgcttttct tttaattgtt cagctagttg
     2881 ttttaaatcg gcgtgctttt catcaggcaa gatgacaaga tagatttgtt ttccttttct
     2941 tgatttcaat aatagatttt ttacttgcgg accaggtagg gaaaaaggga catttttgac
     3001 tgacgtaatc gcaggatgat cgactttttg aaaagagata cccagttttt ttagtagttc
     3061 ataagcttct tgttcggttg cacgactcat cgcgcatcac cctccttttt ttattagtgt
     3121 atcataaaaa agagttgaca aatgaaaaat aatcatgtaa attacacgta aagttaaaac
     3181 aaatgacatc gaaaagaaga gtagccgtta ggtgttttta gagagtctct ggttggtgga
     3241 aagaggcagc agttaaccgt gaaacacatc ttggagtcga ttttctgaag cgtatagtag
     3301 gaaaatccgg ggagcccgtt acagctgcgg tcgttgctag atgaccagat aaggctggct
     3361 tttccgtgag gagtcggtaa aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg
     3421 gatcaaagga ctttttgttt tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa
     3481 ggaaaaaata aacagaggtg gaaccacgta taatacgtcc tcttggcatt tgccaagagg
     3541 atttttttat tttaaaggag gaaaagaaga tggagtattt gattgaagtt atgccgcaat
     3601 tattagtagg agcactcaca acattgaaat tatttttatt taccttgatt ggctcattac
     3661 cattagggat tgttttagca ttaggattgt ctttgcattt taagccgcta cgctacttgc
     3721 ttcaaacata tgtatgggtt atgcggggaa caccgctgtt gttgcaactg atttttattt
     3781 tttatgggtt gccgacgatt gggatcgttt ttgatcgttt cgaagcagca ctcattgcct
     3841 ttatcttaaa ctatgcagct tattttgcag agatctttcg tggagggata caatcgattc
     3901 ctcaaggtca atatgaagca gcgcaagttt tgcaattaag tccctttcaa acggtgacga
     3961 aaatcgtcat cccacaagtg acaaagattg ttttaccttc tgtagggaat gaagttatca
     4021 acttggtcaa agattcttcc ttgatctata tattaggttt aggtgacgta atgcgtgctg
     4081 ggaaaatcgc gatggaacgc gacgcgactt tactgccttt gctaggcgtg gcaatcatct
     4141 atcttacatt gactggtatt ctaacaatcg taatgaaaca agttgaagga aaattaaatt
     4201 attaccgcta actgatagga ggaagaaata tgattgaatt gaagaaagtg aacaaagcat
     4261 ttggtgaaaa aaaaataatc aaagatttga atctaatcat tccagatggg aaaacattag
     4321 ctatcgtagg tccttccggt ggcggtaaaa ccacgctttt acgtatcttg gctggcttag
     4381 aaagttctga cagcggagaa ttttatttag acggtgtacc gttcaaccca ttcgagctta
     4441 aaatgaaaga acaagttgta ggagtcgtat tccaagactt ccagctattt ccacatctga
     4501 atatttttga aaacattacc ttagcaccaa aaatggtatt gaaacagaca aaagaagaat
     4561 acaccaaaaa agcagaagaa ttagcggctt cattagggat tagtgattta ttgaatatct
     4621 atccttatca gctttctggc ggacaaaaac agcgggtagc gattgccaga gcattggcta
     4681 tgaatccacg ggtattggct tatgatgaac caacaagtgc tttagatcct gaattacgcc
     4741 agcaagtcga gcgtttgatc ctatcattga aagaagatgg ggtaacccaa attattgtca
     4801 cccatgattt agtatttgca gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc
     4861 attaagaaag gagaagatag agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg
     4921 tcttcttctt tttgcaggct gttcgaacaa aaaaacttca gcagataatt gggcgactat
     4981 caacgaagag aaaaaaatcg ttataggttt agacgataca tttgttccta tgggatttag
     5041 agataaagac gggaagctca caggatttga tattgattta gccaaagcag tctttgaaca
     5101 atatgggatt acggtagact ttcagcccat tgactggtca atgaaggaat ttgaactgaa
     5161 caatggaacg atcgatttga tatggaacgg ctattccaaa acgccggcaa gagaaaagaa
     5221 agtgcaattt acaaaacctt atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc
     5281 aggaatcaca aactttggac aaatgaataa taagagatta ggtgcgcaaa atgggtcatc
     5341 tggttatgat gtatttacga aacaaccaga agtattaaaa gagatagtca aaaaccaaga
     5401 tgctgttttg tatgatagtt ttaatgaagc attgatcgat ctgaaaagca gacgggtcga
     5461 tggtctgctg atggataaag tatacgcaga ctattatttg aaacaaagaa acgaactaga
     5521 agacttcaac ataacaaaag gttcttatga gagtgaggat tttgcagttg gtgcacgcaa
     5581 gtcagatagt acattagtga aaaaaatcaa tgaagcccta gatcaactag agaaaacagg
     5641 tgaattccaa aaaatatccg ataaatggtt tggcgaagat gttacgccga gaaattaaca
     5701 aataggggac tgtgacaaaa gacgtgtcac agtccctttt tccgaataaa cagtgttctc
     5761 ctgccaactc cttgtgactc ggagctgaat ggcaggctca caacctcttg gtaaacggtg
     5821 ttcaaaagcc agtttttcgg tattaagcca aatttatgca aaaatgtaga aagccatttt
     5881 cacaaaattt ttcttactac tcgaaactaa gcgacttttt cacaacctcc tttacataat
     5941 tttattttgt tgtggaaggg gtttcgtgtt attctaaaag aggaacgatt gaaggttata
     6001 ggaaaggaag agatgaatgg aacacaaaga acttacctca tttccaaaag attttctgtg
     6061 gggctctgct tctgcagcgt atcaagtaga aggagcttgg caagaagatg ggaaaggaga
     6121 atcagtctgg gatcgatttg tacggatacc tggaaagaca tttaaaggaa cgaacggtga
     6181 tttggcagta gaccactatc atcgttataa agaagatatt gctttaatga aagaacaagg
     6241 tctgaaagct taccgcttct ctgtagcttg gacacggatt tttcctaatg gacgaggaga
     6301 agtcaatcaa gctgggctcg ctttttatga gagattgatc gacgaattga ttgaaaacga
     6361 tattgaacct atgttgactt tatatcattg ggatcttcca caagcattgc aagatgaata
     6421 taatggttgg gaatcacgtc agatcattga agatttcact cattatgcag aaacattatt
     6481 tgaggcattc cgcgggaaag tccattactg gattagtttg aatgaacaaa atatcttcac
     6541 ttcattaggt tatttgttag cagcacatcc gccaggtgtg acagacccga aacgaatgta
     6601 tgaagttaac catattgcta atttagcaaa tgcatctgtc atcaataaat ttcacgagtt
     6661 agaaatgcct ggaaaaatcg gaccaagctt tgcgtatacg ccaaattatc caattgacag
     6721 taatcctgaa aatgttctgg ctgctgaaaa tgcagaagac ctgatggcca attattggat
     6781 ggatgtctat atgtggggaa aatatccgat tgctgccatg aggttcttag aagaaaaagg
     6841 atgggcacca acgattgaag ctggcgatgc agaattgcta gaatctgcta aaccagattt
     6901 tcttgggatc aattactatc aaacggcgac gaatgcattc aatccattaa acggagttgg
     6961 tgctggaaaa atgaatacga caggaaaaaa aagttcatca gaagaaactg gagttcctgg
     7021 aatgtataaa aaagttgaga atccatttgt cgaacgaaca aattgggact gggagattga
     7081 cccagaagga ttaaggatcg gactgcgtcg tatcacaagc cgttaccgca tgccggtact
     7141 gattacggaa aatggcttag gagagtatga caagttgaca gaagataaac aaatccatga
     7201 cgactatcga atcaattact tacaaagcca cgtcaaagcc atcaaagaag caatttcgga
     7261 tggagcagaa gtcctaggct attgcacctg gtcttataca gatttgctta gctggctgaa
     7321 cggttatcaa aagcgatatg gatttgtata tgttgatcaa gacgaaacac aagaaggatc
     7381 tttggaacga tataagaaag acagttatta ctggtaccaa aaactgattg aagaaaatgg
     7441 agagaacctt tagttcacat acttaaaaca agaaggtacg gctggatatg gccgtgcctt
     7501 ttcttgataa aatcatttag aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga
     7561 aagcgtcagt tctttcaaac aaaactttag ttcccttact tttttttgta taatgaaact
     7621 tgatgaaatg agggataata aatggcacaa aagaaacggc ctactaaaaa aaggaaaaag
     7681 acgaaaaaaa cgaaaaagca aaaacagcag caggaacaaa tgctgacgat tggtgttggg
     7741 tttgccttga ttttatttgc tgtcttcggc tttttaaaat taggtttttt aggaatattg
     7801 attgcaaacg gctttcggat cattgcagga aatacttacc aaatcctctg tcttttatta
     7861 gccgtattag gattttggat tgtcattaag aatacagagt tttcaattgg taaaaaccga
     7921 cgctggtttg gcggcatact cttttatttc ggtatcttat tattgttaca tgcccattta
     7981 tttggcaaac tccatactgg ggaaccaaat atcatgggga cgacatggga tttgttagcc
     8041 agtgatataa aacaaagtca agtagacaat aacgttggtg gtgggatgat tggagcaatc
     8101 ctttatcatt ttacttactt cttgattgca cagcctggaa gttatttagc agcagtgcta
     8161 ttactggcag gaggagcatt cctgttcagt aatctggaag gctatcagct gttaaatggt
     8221 atccaatcaa ttggagaacg agtacaggaa ttattggaag gtgatcctgc caagcaagct
     8281 cgaaagcaag cggcaaagga agagagaatg aaacagcgcg cagaagcgaa agaagcaagg
     8341 cgcttagctg caaaagaagc tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg
     8401 gaaaatactg ctgatgaatg gcaggaacca gaaaatacag agccggagca gctaagtttt
     8461 gttccaatcg acagttttca agaaaatatc catcctgcga atctagagaa accagtacca
     8521 gatacaccaa aacagaccaa tacagcagaa ggtttttcag atgagttgcc agaggatgac
     8581 ggtacctcat tagaatttga gattgaagca gaacaggaaa atcaagacta tgaattaccg
     8641 acagttgatt tgcttgattc tataccaact gtggatcaaa gtgatgagta taaaaaaatt
     8701 gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc
     8761 gtcaaagcca gcttaggacc atccgtcaca aaattcgaag tccagccggc tgtaggggta
     8821 aaagtaagta aaattgtgaa cttaacagat gatatcgcgc ttgctttagc ggcgaaagat
     8881 gtccggatgg aagcaccgat tccaggtaaa tcactgattg gtattgaagt accaaatgga
     8941 aagatcagta tggtttcctt tagagaaatc atcgaagcac aaccaaacca cccagataaa
     9001 ttactagaag tacctttagg acgtgatgtt tctggacggg tacaaacggc tgatctttcg
     9061 aagatgcctc acttactcgt ggctggttcg actggtagtg ggaagtcagt agcaattaat
     9121 gggattatca caagtatatt aatgcgtgct aagccccatg aagtaaaact gatgatgatc
     9181 gatccgaaaa tggttgaatt gaatatgtac aacggcatac ctcatctgtt gacgccggtc
     9241 gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa
     9301 cgatatgaaa aattcgcggc aaccggagta agaaatattt ctggttacaa tgaatttgtt
     9361 caacagaaaa acttggaaaa cggaacgaaa catccaacct taccattcat cgtagtgatc
     9421 gtagatgaat tagctgattt gatgatggta gccagcaatg aagttgaaga tgcgattatt
     9481 cgtttagcgc aaatggctcg ggcagcgggg attcatatga tcctagctac tcaacgtcca
     9541 agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg
     9601 gtatcaagcg gtacagattc tcggacgatc attgattcta atggggcaga aaagctctta
     9661 gggcgaggag atatgctatt cttgccaatg ggtgagaata aaccgatccg tgtgcaagga
     9721 gcattcattt ctgaccacga agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa
     9781 gctcattatg aagagaaaat gatgccgaca gatgaagttg aaacgacagg tgctccagaa
     9841 cagccacaag atgagctgtt tgaagaagca aaagcacttg tcgttgaaat gcaaacggca
     9901 agtatttccc ttctccaaag aagatttcgg atcggttaca accgagcagc acgtttagta
     9961 gatgaactgg aagctcatgg agttgttggc ccgtcagaag gaagcaagcc tcggaaagta
    10021 ttcattgagc aggaagaaac atcagaaacg ttgccggacg ataatgacat gtaaaacttg
    10081 tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac aaggcatgat gataccaaaa
    10141 gaaaaaatgg tttttttcca tcttttctaa tacggtatga tgtcttgtct ttttagtttt
    10201 tgtgagatta aagttctatt cgatttattt gtgaagtatt gcattaagga aatgaatcat
    10261 gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat actttggaaa aacagtccat
    10321 ttatggcttg acgaatgaag aattaatcaa ttggtttata gaaaacggcg agaaaaaatt
    10381 ccgagctgcc caagtttggg aatggttgta tcaaaaacgt gtaagtaatt tcacagaaat
    10441 gacgaatcta tcgaaacaat tgatcgaaaa attatcggag cattttatca tcaatcctct
    10501 aaaacagatg gtcgtccaag aagcttcgga tggtacagta aaatatttgt ttgaactccc
    10561 tgataaaaac atgattgaga ctgtactgat gagacaagaa tatggcttgt cagtttgtgt
    10621 gacgacacaa gtaggatgta atatcggctg tacgttttgt gcaagcgggc tgctcaaaaa
    10681 gaaccgtgat ctgactgctg gagaaattgt tgcacagatc atgatggtcc aacattattt
    10741 tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt gtaatgggga tcggtgagcc
    10801 atttgataat tacgacaatg taatggactt tttgcatatc atcaatgatg ccaaaggatt
    10861 ggcaattggt gctcggcaca ttacagtttc cactagtgga ttagcacaca aaatcaagga
    10921 attcgctaat aacggcttgc aagtcaatct ggctatttcc ttgcacgcgc cgaataatga
    10981 ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca attgagaaat tgatggaagc
    11041 tgtggatgag tatttagaga aaacaaaccg tcgaatcacg tttgaatata tcatgttgaa
    11101 tcaagtaaat gatagatcag agcatgcgca acaattagca gatctattaa aagataagaa
    11161 aaaattagca tatgtcaact tgattccata taatccagtc agtgaacatg atcaatattc
    11221 tcgcagtcca aaagctgatg tattgagatt ttatgatgta ttgaagaaaa acggcgtaaa
    11281 ctgtgtgata cgtaaagaac atggtacaga tattgatgca gcttgcggac aattgcgaag
    11341 caagcaaatg aaaaaaacag ttgccaaata atcgtatcct actcgttttt tcaattacca
    11401 taaaaggagc agttttctta attaaggaac tgttcaactt aaataagaga aaagattcta
    11461 tagcgaaagc tataaagctt tttttgacaa gaaaaaccct catcaagcaa agaaatgtcc
    11521 tcaatttgat gacagcctgc ttgatggggg ttttagcaat tatttaactg attttcttat
    11581 atccttgctg gctttcttgt gtagcatgat aagtttctac gtcaagaatg gcctgtttaa
    11641 atttttctcg ggtgatttca taaggcacat gtttaatatc atttgttgcc atggctttat
    11701 ctaagattgc ttctaaatcg gtcgtgacat tgagattcat gtcggttaac gatactggaa
    11761 gttggttctt tttcataaac acatagattt tatcaaacaa atcgaactgc tgatccatca
    11821 tcaataaggt cagtacacca taactgacag atgctccgtg aaggtatccc tccatttttt
    11881 ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc agaattatag ttattgttaa
    11941 ctaaaacaga aaccaatccg gtagtaacaa tgatatccga aacgacttgt tcaattgcat
    12001 atgagggacg atcgtcggca ctatctttca atgcttgcgt accataagtc aagagacgat
    12061 caatgcacat ttttccgatt tggattccta atctatccgt gtagcctaga tcatcgccac
    12121 gagcagaaaa agtagattcg acttctttgg ataaagcatc gccaatacct gcccataagt
    12181 atttatcagg tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca ggtggttttg
    12241 aaggaaagaa gagtccgtcc attacgcctt cttcgttata gatgacacat accgcagtga
    12301 cgggcgaaca gttcgaacca atagtgggaa aggtgaagac tggtttagct agtttttcac
    12361 caatgacttt tacagtatcg caagcacggc caccaccgac tgcaaacagg acgtctgctt
    12421 tttggacact ttcatgctgg atcaactgtt caatatttcc ataagttgaa ttacccccgt
    12481 accagagact ggcgacaacc tctatttcct gtgtcaaagc tacttttatt ttgttttctg
    12541 cctttgaaag tgcagtcttc cctccaatca gcactacttt tgtcccatat tctttgacga
    12601 ttccatttgc tttttcatac gcatctgttc caatggtata atttggtaag aaaacacttt
    12661 gactcatatt catttccttc tttcttcgtt gaaacgggtt aaaattttaa agagtgatcg
    12721 atctgactgc ggctgtagat ggagtagagg tgaacctaag acaggataag tgatgaataa
    12781 ggtggcatga acaccttgaa atcatcaata agtatcctaa aaacgaacaa gtacaaaaac
    12841 attgaaaacg acaataatga atttttgttc ctcttagtat ttctaccgat tccctttgct
    12901 tttttgtaat tatctaaaaa ttctaaaaat taagattatt atatgaaaac aggttttatt
    12961 tgtcaattca taattggttt tttttaaagc aaaagataat ctatatcatg atgaacaaac
    13021 agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc
    13081 tgtataattt tttattttat aagtaagata aataatgaaa gaatagagat actgattggt
    13141 agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa
    13201 gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa
    13261 gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga cagctcggca
    13321 gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat
    13381 gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat
    13441 ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac
    13501 ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta
    13561 gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct
    13621 tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc
    13681 gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg
    13741 attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc
    13801 aagaaataag aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat
    13861 ttccgaagaa gctacttttg gaacaccgtg tac
//
LOCUS       DAYYFD010000073        13781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000073 DAYYFD010000000
VERSION     DAYYFD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.073"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..2106)
                     /gene="glyS"
                     /locus_tag="ACHA25_002112"
     CDS             complement(31..2106)
                     /gene="glyS"
                     /locus_tag="ACHA25_002112"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8763395.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2108..3025)
                     /gene="glyQ"
                     /locus_tag="ACHA25_002113"
     CDS             complement(2108..3025)
                     /gene="glyQ"
                     /locus_tag="ACHA25_002113"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8763396.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3409..4221)
                     /gene="recO"
                     /locus_tag="ACHA25_002114"
     CDS             complement(3409..4221)
                     /gene="recO"
                     /locus_tag="ACHA25_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8763397.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4364..5263)
                     /gene="era"
                     /locus_tag="ACHA25_002115"
     CDS             complement(4364..5263)
                     /gene="era"
                     /locus_tag="ACHA25_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8763398.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5278..5673)
                     /locus_tag="ACHA25_002116"
     CDS             complement(5278..5673)
                     /locus_tag="ACHA25_002116"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8763399.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5657..6133)
                     /gene="ybeY"
                     /locus_tag="ACHA25_002117"
     CDS             complement(5657..6133)
                     /gene="ybeY"
                     /locus_tag="ACHA25_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8763400.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6150..8342)
                     /locus_tag="ACHA25_002118"
     CDS             complement(6150..8342)
                     /locus_tag="ACHA25_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8763401.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8366..9337)
                     /locus_tag="ACHA25_002119"
     CDS             complement(8366..9337)
                     /locus_tag="ACHA25_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8763402.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10043..11422
                     /gene="brnQ"
                     /locus_tag="ACHA25_002120"
     CDS             10043..11422
                     /gene="brnQ"
                     /locus_tag="ACHA25_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8763403.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11576..12022)
                     /locus_tag="ACHA25_002121"
     CDS             complement(11576..12022)
                     /locus_tag="ACHA25_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8763404.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12049..12225)
                     /gene="rpsU"
                     /locus_tag="ACHA25_002122"
     CDS             complement(12049..12225)
                     /gene="rpsU"
                     /locus_tag="ACHA25_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8763405.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12387..12815)
                     /locus_tag="ACHA25_002123"
     CDS             complement(12387..12815)
                     /locus_tag="ACHA25_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8763406.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12907..13767
                     /locus_tag="ACHA25_002124"
     CDS             12907..13767
                     /locus_tag="ACHA25_002124"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8763407.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3766 a         2870 c         2490 g         4655 t
ORIGIN      
        1 ctgttctctg gtttatttac atattagaaa ttattttgtg atgatttgat ctaaacttgc
       61 tagtgacaaa gccatggcag caatttgact taattgcgtt aatcggttct tacgtactgc
      121 tggttcttct gccatgacca tcgtctgatc aaaataattt tcgatcagtg ggcgaaggct
      181 tacaagctga tcataatttt cgttgatcgt ttggtcagca aagcttgttt tcattgcctc
      241 tactgctttg tgcagttctt tctccgcttc gttttcgaac agttcagggt ctacttctga
      301 ttgtttttca agctccgctt ttttcgctaa gttcacgaca cgtgtcaaag cttccatcga
      361 tggtttgaag tcttcatctg caagatgctg cttgaacaat tgtgctgctg caaatacttt
      421 tgtcagatct ttttgttctg cattcaaaac agcttcgatg acatcatgac ggatattctt
      481 cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacctgtt gttgtcccgc
      541 tgacaagcca atgccaaaac gatccgtatc tttactgata acttcactaa tcgtttcttg
      601 cagaacagac aaaggaaagg cccatttttt cgattcgatg attcgaacga caccatatgc
      661 ttgacggcgc aaagcatatg gatcgtttga gccacttggg atcatcccaa ctgcaaagaa
      721 agaaaagata ctatctaatt tatcagcaat cgctaaaaca gaacctactg tagtctcagg
      781 taaatctcct tcactggata taggcatgta atgttcacgg atcgctgttg ctacggctgg
      841 tttctcgcct ttaagcagag catatttttc gcccatgatc ccttgtagtt cagggaattc
      901 tcctaccatg ttggttacta gatcaaattt atagatttca gcagcgcgtt gcagatccat
      961 caattcttcc tcagatagtc ctacttgttc gcctatgatg cgagcgatga cttgtacccg
     1021 ttgcattttc tcatagatgc taccgatttt ttcatggaaa gtaacagttt ttaatttttc
     1081 aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg catcttctaa
     1141 acgagcagtc aatacttttt gattgcctcg taccacattt tccaatttta cccgatcgcc
     1201 attacgagca gaaataaaat gaggaagcaa cataccttga tcgttccgta cttcgaaata
     1261 acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagata gatatttttc
     1321 ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt cttctagtaa
     1381 atcttcatct agatcaatga tccagtgatt tttttctgct aattcgtttg cttgtgtgat
     1441 gatcatttct tttctttttt gcggtgaagc aataacaaat tgttcattca atttttcttc
     1501 gtattgtttt gcatgcgtaa atgtcacatc ttcgcctaag aaacgatgac ctcttgaagc
     1561 tctgtctgtt ttgacatcca agatctcaaa cgggattatt tcgtcatcta acaatgccac
     1621 gatccagtga atagggcgaa tatattcaaa atcgtattct gcccagtgca tcgttactgg
     1681 gaaagtgagg cttttaatga catctgctaa acctgtcaat acttcttttg cttctttgcc
     1741 gtgagtgtat ttcgttacat agacatattc cacgcctttt aattctctaa aagtgatagc
     1801 atctgtcgtc agaccttgtc cgcggacaaa tccttcagct gctttcgtcc agtttccatc
     1861 tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcgctgt ctgcttgacg
     1921 ttctggaatg tttgttactt tgattgctaa gcggcgcggt gtagaaaacg tttcgatcgt
     1981 atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta attgtttcat
     2041 gcttggtgtg actacatgag caggtatttc ttccagcccg atttcaagta ataagtcttt
     2101 tgccatctta ttcagcctcc ttcacgtttt ctggatttgc tgtgttttta ttcaataaag
     2161 ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga gccatgttgc
     2221 ggatacggga aagataacct gcacgctctg ttacggatac tgcaccgcga gcatctaata
     2281 aattgaatgt atggctacat ttcaaaatat aatcatacgc tggatgaaca agattttcat
     2341 cgatacagcg tttagcttct ttttcaaaac gatcaaaatt gcttagtagc aggtcttggt
     2401 cgcttacttc aaaagaatat ttagaatgtt catattctgg ttgtttgaag atctcaccat
     2461 atttcacgcc atcagcccat tccagatcat aaacactttc aacttcttgg atataagaag
     2521 caagacgttc tagtccataa gtgatctcag aagtaactgg tttacaaggt agtcctccga
     2581 cttgctggaa gtatgtgaat tgtgtgatct ccatcccatc aagccatact tcccagccca
     2641 caccggcaca acccatcgat gggttctccc agttgtcttc aacaaagcga atatcgtgtt
     2701 ctaatggatc aattcctaaa agacgcaagc tttctaaata aagctcttgg atattttcag
     2761 gtgaaggttt cattacgact tgaaattgat gatgctgata caaacggttt gggttttctc
     2821 cataacgccc gtcagctggt cggcgggatg gctctacata agcagcattc catggttccg
     2881 gtccgattgc ccgtaagaat gtatatgggc tcatcgtacc tgctcctttt tctgtatcat
     2941 aagcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat
     3001 cttggacggt caatttttta ctcatctgtt ttgccccttt caatttttaa gcgttgctgt
     3061 caataagaat acaggaaaga aaacgacaaa aagtccctat gtctgattac agacataggg
     3121 acgaataatt tcgcggttcc accctacttc cgaataaaga ttcggcagct tcatttatcg
     3181 tactcgcaag tgcctcttca aaaatcgttt gggtccggct tccactctcc cgaactcgct
     3241 aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca gttttctggt
     3301 ttgtataata gatcctctca gtgaacgatc tatttaatct acggttaata atagacggaa
     3361 gtccctgtat ttgtcaaata gttttgattt ttttgttgta tctttcattt attcttcttt
     3421 ttcaggtatc tctttttcag gcatattttt ctctggcgtc tttaatacgt gtgcccaaga
     3481 cttcatttga tcaatgaact ttttgctttt tagatgaata ccgacatatt catcatacaa
     3541 ctggtcaatg aggtcacgga ttgcctgctt cgtttcttct ttcaagtgga ttccactgat
     3601 cttatcataa gaaatagccg aaaacattcg aacgaaataa acagcgcgag gatctgcatg
     3661 ataacggtgt tcgtccattg gccagtgttt ctcgcatatc acaccgccat atttggaaga
     3721 atagtcaaat tttccttttg tttccccgca tacgacacag tgcgtccaaa ttggcgcgat
     3781 tccgaagcgt tgcagtattt gcacttcaaa gatattcgtg atgatttctg catcatctcc
     3841 ttggtccatc cgctgcagag cttgttgcgt aaattggaat aaatgcgggt catattgatg
     3901 atcttcgatt gctgcatcta ctagatttag caaatacgta ccataaccgc tcaaaaagat
     3961 atcttcttgg atgttccgaa acgcatggac gtctttgaca ctgcttaaaa acgacaaacc
     4021 ttcacttcga aaattaccga tatagactgc ttcggtaaag gggagcaaag ccggagctaa
     4081 tggctgattt tgccgatgga ttcctttgat ataaaacatc attttgccag aagattccgt
     4141 aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa tcaagccttt
     4201 cgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa aagcagattc
     4261 tgccgatcca gattatttat gtttcgatct tccgctgttt ttctcaacgt acggattttt
     4321 atccagttta tttctatacc taacctataa gaagtccaga atatcagtac tcgtcttttc
     4381 ggtagccgaa gtcttgcaga tagacttttt tgtctcgcca gtctttttgg actttgaccc
     4441 aaagttctag aaatactttg tctcctaaaa gattttcgat atcctttctt gctttcgttc
     4501 ctacttgctt cagcattttt ccgcctttgc cgataatgat tcctttttgg ctatctcttt
     4561 cgacgatgat cgtggcctgg atatggattt tatcattctc gtttcgcttc atcgaatcaa
     4621 caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga
     4681 tcaattcaga aacgatgaag tattcaggat gatcagtcac ttgatcatct ggaaaatatt
     4741 gtgggccctc tggcatttca ctgactaata catccattaa gcgttcgaca ttgtttcctt
     4801 ctgtagcaga gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa tcttcgatga
     4861 ttcccagcaa ctcgtctgga tggatcttat cgattttatt gataatcaga taaacaggag
     4921 aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccggccacgt ttttgatcag
     4981 cactgatcat aaataagact gcatccactt ctttcaacgc actgtaagct gcttctacca
     5041 taaaatctcc tagacgatgc tttggtttat ggattcctgg tgtatcgata aaaatcagct
     5101 gagcttctgg tgtagtatat actccttgga ttttatttct agttgtctgt gccttgtcac
     5161 tcataatggc aattttttga cccacgattc ggttcaataa tgtggatttt cctacattcg
     5221 ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatacgtt ctccttttta
     5281 attaaacaac aaatgatata ttttagggac aaataaaatc aacccaatga tgacagcaaa
     5341 acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc caatcggatg
     5401 aaaatgaaaa tccgtaaaca tgtccactac attttcaaaa acagtattga tgatctctac
     5461 tatccaaacg agaaaaacag ctaaaagcag ccataaccac tctatccgat caagctggaa
     5521 aatcacgccc ataaataaag caaggatacc gaacgcaaca tgtttcctca tattgcgttc
     5581 ttcctcaaaa acggttttga ctccttggac agcaaattcc acagacatca ggaaatgttt
     5641 gtttttctct acgtttttat cttttgagtc cataggcatc caagatctct ttctgtaaac
     5701 caaacatttc tttctcatct tctggtgtca tatggtcata cccgttgata tgcaagaatc
     5761 catgtaccgc taaaaagcca agttcccgat catatgaatg gccgtaagct tctgcttgtt
     5821 cagcagcacg ttcaatagag atcatgatgt ctcctagtac acgaggtaat ggattctctc
     5881 catcttcaaa aatcactggg atttcgtctt cgccttcttc ttccagagca aaactgatga
     5941 cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt gccgcattgt
     6001 ccatgaaagt aacagacatt tctgtttcct ctggcagctt taaataagat gctgcaaatt
     6061 gcagcaagtc atcgatttct ttcattttct cttctgagac ttggtttgtt tcatccataa
     6121 aggtaatatc cattatacat cgcttctttc tattcttctt gttcttcctt tatcttctca
     6181 acttctgttt tcattttagg atatttgatc cgagaatgga aggtgctact taaaccgctg
     6241 attaatgatt tttccacttt tcggatttct ttcaaggtca gtccactatc gtctaattga
     6301 ccatcggaaa tacgctcttc gataagatta tggacaaaac tttcgatttt ttcgctcgtc
     6361 ggatgatcca ttgcccgtac tgcagcctca caactgtcag caatattgac gatccctgct
     6421 tcacgacttt gcggttttgg tccaggataa cggaattgct cttctgttac ttccggattg
     6481 cgttctttcg ctttgatgta gaaataacgc attagcgtgg ttccatgatg ctgtttgcaa
     6541 atatcgatca ccatttgcgg catattataa tcttccaata ttttggctcc atctgtcaca
     6601 tgtccaaaga taatatgttt actgtcttct ggcaataaga aattgtgcgg gttttccgct
     6661 cctgaaggca agttttcaac gaaaaagcta gcatgtttga tcttgccgat atcatgataa
     6721 tagcaagcta ctcgagttag caaagaacgt ccgccgattt ctgctacagc gttagcactt
     6781 agattagcta ccatcatact atgatgataa gtacctggcg cttcttccag caactgtttc
     6841 aataaaggat gatttggatt gctgagttca tttaatacaa ttacactgtc atcattgacc
     6901 atcaattcaa tatatgggtg tagaccgatc gtcagcaaga atgagaagag tgcaccagtg
     6961 aacccgcaga cgagcattaa ccaagtcgtt gcatcaccaa agctcattcc ttgatagacg
     7021 atcaaaatga catccatcat cacaggaaaa ataatgaccc acatggcagc agataatcct
     7081 tgttctgaga tccgtttgcg tctgacaatc gttgccatga gcccagagaa aacataagac
     7141 atcaagataa tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa aagtgcggat
     7201 actgcttgga aaataccagc catgatccct gctcttcgat tcaagaaaaa gcttaaaaca
     7261 agtggtgcaa aagcagcggg ataaaacagc gggatataag tagcttgttc cgtttggaat
     7321 aattggaaaa atttcatcag cactatgctg actgacatag ctgtcacata gaaaagcacg
     7381 tattttgttc tttctcctgc attttggaat tgaagtgaat tataaatcag tacgccgatt
     7441 tgaagcaaaa cagctaaaac catggcaatt agcggaaaaa tcgaagttgt ttgattagtc
     7501 agtcctagta attccaattt tttcatagct gaagcatcga tttgattacc ttcacgaaca
     7561 atgatttctc cttggaagat catcactggt tgtacagctt ctcgtgcgga ttgtttcaat
     7621 tcttctgtct ttttctcatt caatacgtca ttcactacga taccttgatc cactaagtag
     7681 cgaatcattt gttgttgtgc tggtgtgaca ttcaaatatt ggatttgctc ttctgcttct
     7741 tgtttaaaag cttccaattc actttcacga acttgtttgc tcatctgttc gtcaatgagc
     7801 ttcagactct catccctcac agtatgaatc tcagtttcag tcatttcaaa tatcgtcgaa
     7861 taaaaattgt tcggtagttt ttgataaaac acaacatttt ctgcattcac attttcaaaa
     7921 tttttcttca gcgcggcgat ccgctcttct gtagtaggct ttgggatatt ttcatcttcc
     7981 tttgccttat caatcttttc gtcataagat ttgttgatgg catcattcgt cttatcgatc
     8041 aattcaaata gttgcttgat ccgattgttc tggtcatctg caagatcttt ttggtaagtg
     8101 tattctggag tgactgcttc tgctgccagt tttctttttt gttcggtttc ttccgtgttc
     8161 tcgatcgttt tgttcgcacg gatactttct tctgctactt ggccttctct gaaatcattg
     8221 gccttttgtt tgacactact aaatgtaacg ccgcagataa tgataaaaaa caagaacagc
     8281 aggaaaggca atgctgcctt tccaagtttt tcttgtagtt tctgcagtgt tttattcaac
     8341 atgtgcttat cctcctaagc aaagcttatt cttgatggag atctgccttt tcataagctt
     8401 tgatgatttc ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat
     8461 cgattttctt gatttgcttt aatgttcgtt ctgcatgaac aagaccactt gtcgtccctc
     8521 gaggcaagtc tatctgactt gtatccccat tgacaatcat ttttgattga taaccgagac
     8581 gggtcaagaa cattttcatc tgagcgactg tcgtgttttg tgcttcgtcc aagataacga
     8641 atgcatcatc caatgttcgc ccccgcatgt aagctaaagg tgcgatttcg attacgcctc
     8701 gttccatcaa tcggttcgta tgatccaatc caaaaatttg gtataaagca tcataaacag
     8761 gccgcagata aggatccact ttttctttta gatccccggg aagaaagcct agattttcac
     8821 ctgcttccac agcaggccgc gtcaaaatga ttttttgcac ttcacctttt ttcaaggcag
     8881 cgattgctaa tactactgca agaaaagttt ttcctgtacc ggctggtcct acgccaaata
     8941 cgatatcatg tttggctacc gattcgacat atttcttttg gccgctgttt tttacccgta
     9001 taggttttcc attacggtct ttgacgatct cttcctcata catctcaaca aaataatcta
     9061 atgtaccttt ttgccccatt ttcaatgccg tcacaacatc gggcgtactt acgtggattc
     9121 ctcgtttgat caattcctgt aatgtccgaa tgactgaggc agccaaggaa atctgggatt
     9181 gctcaccaat gatctgaatg atttctccac gtgtatggat ggttgtttcc gttgtttcct
     9241 caatcagttt tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca tcagcagccg
     9301 tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc ctttttttcg
     9361 attcattctt tcctatttta ccatactcag aagaaactat atagcaaata agaaaaggtt
     9421 ctaaaagagt tgcttaacca taagtaccaa gaaacgttaa aaaactagcc gcttccattc
     9481 tctaaaggtc tcgtttgaat aaaaaaagct gatcatgtaa gctatgcata agatagagga
     9541 caagtattcc gtctaactta gcaagtattc tctgggtatt tttcttgttt caaaaaaaga
     9601 aaacaaaata tttgaaatat tcttgcattt caaaaaaaac aggtgtatta taacgtcaac
     9661 aaataaatat acagcaaagc gtcgaggaaa acagtaggct attcagtgat ttattcccag
     9721 tgagtcatgt aagtgagaaa tgacaataaa tcgaatagac gaacatcatt tccgagtcaa
     9781 tgtcgaaaac gtagatcatt gtggctgcct ccattatcag gctagcggat aactttatcg
     9841 aaaagcgtcc gtgaatgaag aagctttttt aagcgaaaaa aggtggtacc gttctagaat
     9901 ctagagccct tttcccaagt ggaaaagggc tttttttata ttttaaggtg gtggttttca
     9961 tggaatctga tattagtggt atttctagtt tctgcaacgt ttattttgtt actcaaaatg
    10021 ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga agcaattatt
    10081 gatggtcagt tcacttatct tcgggatgtt cttcgggtca ggtaatctga tctttcctgt
    10141 acacttaggt caaatggctg gatcaaactg gatcagcgct ggtgtaggat ttgccatctc
    10201 aggcgctctt ttcccattat tagctatctt agctgtagta gtgacaaaaa gtgatggtct
    10261 gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttga tccttgttca
    10321 tttaacgatc ggaccattct ttggtacacc gcggacagca gcaactgctt ttgaaatggc
    10381 tgctaaaccc tttttgcctg aaaaatatga ttcattaggt atgtttttat tctctgctct
    10441 tttcttttta cttgcttatt tccttactgt acatccaaca cgtttggtca aatatgtcgg
    10501 aaagtattta aactccttgt ttcttgcatt attagctatc atttttgtcg tttctttatt
    10561 ccaacccatg gggaatctaa accaatctgc tgcagattct tatcagacga acgctttgac
    10621 aaatggcgtt ttagaaggat acaacacggt tgatgcagta gctttacttg ctttaagtgt
    10681 cacttttgtc catgcagttc gtgatcttgg ttttaaagat aaaaaagtca cagaattaac
    10741 agcaaaagct ggtagcctga gcgttgcgtt agaagtcatg atttattttg gtctggtcct
    10801 tctcggtgcg atgagtttga ataagatgtc tttatcagaa aatggcggaa ctgcactttc
    10861 tcagatcact aatttttatt taggtaactt cggtgctagc ttcttagggg taatggtcac
    10921 attaggtgtc ttcactactg ctatgggact cgttgtttct tttgcacaag atttccataa
    10981 actgttccct aaagtcagtt atatgacatg gctgcggtta acgacttttg tctcttttgt
    11041 cgttgcgaat gccggattgg ataatatcat ccaatggtct ttaccagtat tgatgctttt
    11101 gtatccattg tcacttgcgt taatcttact ttccttaaca gcaaaattct tccaaaagag
    11161 tccatttgtc tatcaagtaa cgatgttctt ttctgccgtt ccagccgtat tggatatgtt
    11221 ggcaaattcg cctgcattag tgagtcagca aactgtcgta gcaagcttac taggatttta
    11281 tcatcattat gttccttttg ctgcattagg tcttggctgg gtcgtcccaa cgttgcttgg
    11341 ttacgctggc agcttgctct tttattatgt atatcgcgta tcaggatata aacaagaagc
    11401 aagtgagtta ccagaagaat aaccaaatca aacacaattt aacataagag atattaaaca
    11461 ggaaaaaaac agctagaaga aagaataaga ttctttcttc tagctgtttt ttagttcttt
    11521 ttttacgaac acaaaaaact gcggataatc actgtccgca gtttaaatga taaaattaag
    11581 attgtaatag ttctttcaca atcgcattca cttggtttcc atctgctttt cctttgactt
    11641 taggcataat tgcacccata actttgccaa attctttcat tgatgaagca ccagtttgag
    11701 tgattgcttc ttgaacaagt tgtcgaattt cttcgtcgga aagttgttcc ggcatatatt
    11761 tttcgactat tacaatttca ctttttactt tttcagctaa atcgtcacgt cctgcttctt
    11821 caaactcatg caaagaatca cgtcgctgtt tcatttcacg agacaacacc gttagttctt
    11881 cttcttctgt caagtcgtga ccttctttaa tctgctcgtt ttgtatagat gacttgatca
    11941 ttcggatgac ttgtaaagat tccttatctt tcgctttcat cgctgctttc atgtcatcgt
    12001 ttaatctact tagtagtgac acactaccaa ctccaattca tcacagaact agaatttttt
    12061 gcgttttcta gctgcttcag attttttctt gcgtcttaca cttggttttt cgtagaattc
    12121 acgtttacga gattcttgta aagtacccgc ttttgacacg gaacgtttga agcgacgaag
    12181 agcatcgtca agtgattcgt ttttgcgaac gactgttttt gacatgttaa ttccctccct
    12241 ccgagcttaa aaagtaaccg taattgttat tgaaatatat ctcaaaaaac aagactgtcc
    12301 tgtaaatatt atatctaatg ctgttttgac cgtcaaggaa atcttgcttt tttttaaaat
    12361 tatcaaatgt tattttattt aaatccttat tgacaatttt cacatcttcc gaaaatctcg
    12421 aatctatgcc cttcgatcgt gcatccacct agctgttctt cgaaataatt catcggacac
    12481 atatggattt ctttcgttgt tccgcacact gtacagataa aatgatggtg atggccgatt
    12541 ttcccctgac agcaatgaaa acgaaacttc atttctccat ttaaatcggt ttcttccagt
    12601 aaatcgatct ctgcaaaatc atggagattc cggtaaatcg tatcataact tacccctttg
    12661 aattcctgat tcatatagtc aaaaacttct ttagcagata cgtagcggtt acgcttcact
    12721 aaataagtta acaatgcttc tcttttttta gtatatttaa aaccgttatt tttcaatgtt
    12781 tctaatgatt gttcgatcaa tgtctgtttt ttcatgactt attcctccaa atcaaaatga
    12841 ttacgaagta cgcattctag agtatggtat aataaacata aaaaaaatgc aagtgaggtt
    12901 ttatccatgt cttccgattc tacagaaatc gttacttttt ttgtcatctc cgactctgct
    12961 ggagaaactg cgacaaaact agcgcaagct actatggctc agtatcctac cgttgagttc
    13021 aacctattcc gcagaacgtt cgtgacagat gaaaaaacat taaggcaggc attgcaagat
    13081 gcattagaag aaaaagcact tgtcctgcat acattaatca atcaagaact gatcgagata
    13141 acacgttctt tttgtgaaga acatcaatta tttagttttg atgtgatgac gccaccgata
    13201 tcagaaattg aacgtttaac tggtatcaag ccaattcggc aacctggtgc cctccattta
    13261 ttgaatgaga actatttcaa acggatcaaa gcaatggaat ttgctgtaaa atacgatgat
    13321 gggaaagatc ctcgtggttt tttagaagca gatgtcgttt tgctaggcgt atcccgaact
    13381 tccaaaacgc ctttgagctt gtttttagct aacaaaaatt taaaagttgc caatttacct
    13441 ttgatcccac aagcacatat tcccaaacag ctttgggaga tcgatcctaa aaaaatcgtg
    13501 ggactaacga ataacccaga tattttaaat aatatccgta aagaacggat gcgctcttac
    13561 ggattaaacc ctgatactgc ttattccgat atcgaaaaaa ttagagctga acttgatttt
    13621 gcgaatgatc tttatgaaaa actaggatgt gtagtgatca atgtggcatc tttatctatt
    13681 gaagaaacgg cttcgcttat tttgaatgct ttagacctag aagatcatag ttattatgga
    13741 actgaaacaa cagaaaaaag cgattaatca tttttgtctt t
//
LOCUS       DAYYFD010000074        13761 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000074 DAYYFD010000000
VERSION     DAYYFD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.074"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..296
                     /locus_tag="ACHA25_002125"
     CDS             102..296
                     /locus_tag="ACHA25_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763408.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            565..864
                     /locus_tag="ACHA25_002126"
     CDS             565..864
                     /locus_tag="ACHA25_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763409.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(961..1395)
                     /locus_tag="ACHA25_002127"
     CDS             complement(961..1395)
                     /locus_tag="ACHA25_002127"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8763410.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1627..2337)
                     /locus_tag="ACHA25_002128"
     CDS             complement(1627..2337)
                     /locus_tag="ACHA25_002128"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8763411.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2327..3187)
                     /locus_tag="ACHA25_002129"
     CDS             complement(2327..3187)
                     /locus_tag="ACHA25_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8763412.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3192..3830)
                     /locus_tag="ACHA25_002130"
     CDS             complement(3192..3830)
                     /locus_tag="ACHA25_002130"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8763413.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3845..4144)
                     /locus_tag="ACHA25_002131"
     CDS             complement(3845..4144)
                     /locus_tag="ACHA25_002131"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8763414.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4187..5671)
                     /locus_tag="ACHA25_002132"
     CDS             complement(4187..5671)
                     /locus_tag="ACHA25_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8763415.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5691..6152)
                     /locus_tag="ACHA25_002133"
     CDS             complement(5691..6152)
                     /locus_tag="ACHA25_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8763416.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6170..7237)
                     /gene="ulaG"
                     /locus_tag="ACHA25_002134"
     CDS             complement(6170..7237)
                     /gene="ulaG"
                     /locus_tag="ACHA25_002134"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8763417.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7455..8225
                     /locus_tag="ACHA25_002135"
     CDS             7455..8225
                     /locus_tag="ACHA25_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8763418.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8498..9325)
                     /locus_tag="ACHA25_002136"
     CDS             complement(8498..9325)
                     /locus_tag="ACHA25_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763419.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9893..11128
                     /locus_tag="ACHA25_002137"
     CDS             9893..11128
                     /locus_tag="ACHA25_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8763420.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12127..12387)
                     /locus_tag="ACHA25_002138"
     CDS             complement(12127..12387)
                     /locus_tag="ACHA25_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8763421.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12414..13715)
                     /locus_tag="ACHA25_002139"
     CDS             complement(12414..13715)
                     /locus_tag="ACHA25_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8763422.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4419 a         2822 c         2170 g         4350 t
ORIGIN      
        1 aattgattct ttttatttat cataaagata tagatacata atcattttca cttacatata
       61 tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat gtagaagtaa
      121 aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa tatcctttac
      181 ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa ataaagaacg
      241 agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa aagtaaatcc
      301 aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa gattatagaa
      361 ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt ataccatatg
      421 attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa gatgaatcaa
      481 atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt tttataatgt
      541 atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag tgaactactt
      601 aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca caaagtgaca
      661 gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc ggcaaaagga
      721 atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt tgctttaaaa
      781 aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt atttgataaa
      841 gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg atactaagtc
      901 tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg ttattatatg
      961 ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat aagaaatacc
     1021 acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg ttttctcagt
     1081 atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct ccaaagcttc
     1141 attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga ggatatcttg
     1201 aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa tcttcccttc
     1261 cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc ttgcagtgtc
     1321 ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat cgatcataag
     1381 ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa taatacagtt
     1441 ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg attccttaaa
     1501 attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt gataaatttt
     1561 cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac ccttacccta
     1621 gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat gtttatctag
     1681 taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt tggccatcag
     1741 acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat ccccccatgt
     1801 gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta attttctttc
     1861 cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc ttacttcttc
     1921 tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat gagtcgtgcc
     1981 ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg
     2041 agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg ggagatctga
     2101 agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta ccaccatttg
     2161 atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga ctcctagctc
     2221 acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc gcggcaatgc
     2281 cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac aagacatcat
     2341 agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt tctcttcgaa
     2401 agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa tctagacgtt
     2461 tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga acatcacgaa
     2521 actggcctga atattctttt gttacagggt aagtatcttt taagtggata gcagtgatat
     2581 aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg gacaaattgc
     2641 ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt tctaaaaatt
     2701 ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg atgccatatt
     2761 tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg gaattcattg
     2821 atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg cttagattat
     2881 gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt tctggatcag
     2941 cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg
     3001 agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct aatcgtgcta
     3061 accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt tcttttacaa
     3121 gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa atcccaattt
     3181 gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg tgcggccttc
     3241 atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac atcgatccct
     3301 tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt aaagcctagt
     3361 tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc agataacagc
     3421 gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca atccatggct
     3481 tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc tttttgtgct
     3541 gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc tcctgctttt
     3601 gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc gactagtttt
     3661 ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg taagcaaaga
     3721 attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc tcctaatgca
     3781 tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat cttagttcct
     3841 tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg atttctttga
     3901 ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc tgagtaaaca
     3961 tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa ctttttccct
     4021 ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc attgcttttt
     4081 ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat actaaaattc
     4141 tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat actctctgct
     4201 tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga tttttccgat
     4261 attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca gcataaccta
     4321 ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac atcccgatat
     4381 aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg cctacttgta
     4441 tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg ttattggcat
     4501 aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg acgatcgttg
     4561 gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga
     4621 acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg tcaattcctg
     4681 gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt agttcatcga
     4741 cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg
     4801 tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga atcaaataat
     4861 ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac gaagtagcaa
     4921 ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca atttttttgt
     4981 cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca gctaattttg
     5041 caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca ccttctgtca
     5101 attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa cctaaaatca
     5161 gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc
     5221 aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt
     5281 tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc agtaataaaa
     5341 tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc acagcatttt
     5401 gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt agtccaacca
     5461 agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata aagtaaccga
     5521 ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc
     5581 ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt gttgcaaaat
     5641 acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa ttaattatat
     5701 tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg ctcaaccaat
     5761 ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc ttctggatct
     5821 tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc ttcactttca
     5881 aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac accatcactt
     5941 ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg accgtatttt
     6001 tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt atcgatcaat
     6061 acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc tgggtaatga
     6121 atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct tataaaaacg
     6181 acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga taaaaattaa
     6241 actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc cagatataag
     6301 gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg atctcttttg
     6361 gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact tttgcattca
     6421 atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc cctcgtgggt
     6481 tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt ttggcaaata
     6541 aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt tcgaataaat
     6601 agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc tcttctggat
     6661 cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca ttgatgtctt
     6721 tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga attccccatt
     6781 tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga caattttgat
     6841 gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta acgattaaag
     6901 cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt aaattaggct
     6961 gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc atgccattcc
     7021 catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt ccttcatgac
     7081 ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca ggatccacta
     7141 cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt tctactaacc
     7201 acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc ccatatcgtt
     7261 gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc ttttatatga
     7321 ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt ataataaaga
     7381 cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa gatgtccctc
     7441 aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa aaagaaattt
     7501 tggaactgct tcagcagact gaacaactat caactttcga attagctgaa tcccttgatg
     7561 tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac gagatcatac
     7621 gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat caatctggtc
     7681 ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac gattatgata
     7741 cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt caatctaacc
     7801 atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa gataattgta
     7861 gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg ggcgacatcg
     7921 cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt agcggagtcg
     7981 atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac gaaatgatgg
     8041 tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc ggactgacat
     8101 caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat aaattttgtt
     8161 ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa gtagattcat
     8221 tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat aacaaattta
     8281 tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga tgtatccatc
     8341 caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg cctaaatagg
     8401 aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt tttcgaataa
     8461 acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg ttttcaatca
     8521 cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt
     8581 ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa agcaagtatt
     8641 ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca cataattgga
     8701 ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa tatacaatgc
     8761 agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc tccacataaa
     8821 ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt gcagctgctt
     8881 gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa taaaataatg
     8941 ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat aaaatttgat
     9001 ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt ttataagatt
     9061 cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc ctgattttgt
     9121 cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg caaagcgcac
     9181 ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca gttgcttgtt
     9241 cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg tattctttta
     9301 gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat tatttctcct
     9361 ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca gaataaaaat
     9421 tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt gatttctttt
     9481 tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt gtcatggcga
     9541 gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta taatgacgac
     9601 agcacaccta tcaattaata taattatttt atttttttat gttctcatgt ttatttttaa
     9661 tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa attgttgcgt
     9721 gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga agttgtgaaa
     9781 acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc tgtgtttttt
     9841 agtatactag taagaaacga agggatagac tcattccaat aaggaggatt tcatgaagac
     9901 aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga ttgaggttgc
     9961 caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa cttttgctcc
    10021 tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta cgaaaaaaca
    10081 agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt ttaccaaaga
    10141 aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg agcctcgact
    10201 gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa agattccttt
    10261 agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc atgcgctgat
    10321 gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact ggacaaaaaa
    10381 gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac aaaaagaaat
    10441 gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc tcatcacaac
    10501 gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg ttggtccaat
    10561 ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac agcgaaagca
    10621 acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc cgcgtatgtt
    10681 acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg cacctattga
    10741 ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag aatatctgtc
    10801 tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg aagggaccgt
    10861 ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat ttgagcaaag
    10921 ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa aaccagtgac
    10981 tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc gtcagcaggc
    11041 acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc aagaaatcga
    11101 aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt taatagagac
    11161 tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa gtaaatagtg
    11221 gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat catccttaaa
    11281 ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta ttcttagtca
    11341 aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa gaaagaccat
    11401 aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag catcgctttg
    11461 tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg atttaccact
    11521 ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa tccgatgcat
    11581 actcctatat aaactgccag caatccccac atattgagta gatcatgtca gaatgttcta
    11641 tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct attcccagca
    11701 caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac atattaccat
    11761 tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct atatttgact
    11821 tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa tttccatttt
    11881 ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta aaaaagggct
    11941 gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa agcttgcatc
    12001 tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa tttaagaagc
    12061 aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca accactttta
    12121 gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa aattgatagc
    12181 aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat gcggtacaat
    12241 gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc gattgatttg
    12301 aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata aaaagataaa
    12361 aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca aacttagaca
    12421 gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt atcataagct
    12481 tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat caagataaca
    12541 acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa tgtccatgtt
    12601 ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact gataatagtc
    12661 aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat cataggagct
    12721 ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa acgaccaacc
    12781 tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac gatcgttgct
    12841 cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat atagggtaat
    12901 tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat gatcagccac
    12961 attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga ccacaacaaa
    13021 tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat attggttaaa
    13081 ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct tcgaatcagc
    13141 caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg ggcaacagca
    13201 ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa acgatatact
    13261 tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc caaagaactc
    13321 ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac aggaacttgt
    13381 aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa tccatatgat
    13441 ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc catcgtcatc
    13501 ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca tccaggaaac
    13561 ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa tgttgaaaca
    13621 accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat tggtgggaca
    13681 agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt tcaataaaat
    13741 atgaaccatt cattgcatga c
//
LOCUS       DAYYFD010000075        13670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000075 DAYYFD010000000
VERSION     DAYYFD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.075"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..479
                     /locus_tag="ACHA25_002140"
     CDS             141..479
                     /locus_tag="ACHA25_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763423.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(684..1246)
                     /locus_tag="ACHA25_002141"
                     /pseudo
     CDS             complement(684..1246)
                     /locus_tag="ACHA25_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(1978..2559)
                     /locus_tag="ACHA25_002142"
     CDS             complement(1978..2559)
                     /locus_tag="ACHA25_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8763424.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2742..3368)
                     /locus_tag="ACHA25_002143"
     CDS             complement(2742..3368)
                     /locus_tag="ACHA25_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8763425.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3390..4721)
                     /locus_tag="ACHA25_002144"
     CDS             complement(3390..4721)
                     /locus_tag="ACHA25_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8763426.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4876..5277)
                     /locus_tag="ACHA25_002145"
     CDS             complement(4876..5277)
                     /locus_tag="ACHA25_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8763427.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5363..5569)
                     /locus_tag="ACHA25_002146"
     CDS             complement(5363..5569)
                     /locus_tag="ACHA25_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763428.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5664..7166)
                     /locus_tag="ACHA25_002147"
     CDS             complement(5664..7166)
                     /locus_tag="ACHA25_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8763429.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7184..7639)
                     /gene="argR"
                     /locus_tag="ACHA25_002148"
     CDS             complement(7184..7639)
                     /gene="argR"
                     /locus_tag="ACHA25_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8763430.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7737..8354)
                     /locus_tag="ACHA25_002149"
     CDS             complement(7737..8354)
                     /locus_tag="ACHA25_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8763431.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8575..9948
                     /locus_tag="ACHA25_002150"
     CDS             8575..9948
                     /locus_tag="ACHA25_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8763432.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10023..11135
                     /locus_tag="ACHA25_002151"
     CDS             10023..11135
                     /locus_tag="ACHA25_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8763433.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11207..12844)
                     /locus_tag="ACHA25_002152"
     CDS             complement(11207..12844)
                     /locus_tag="ACHA25_002152"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8763434.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13012..13620)
                     /locus_tag="ACHA25_002153"
     CDS             complement(13012..13620)
                     /locus_tag="ACHA25_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8763435.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3683 a         2882 c         2448 g         4657 t
ORIGIN      
        1 taactaaaaa aataatccaa gaaacattga acctatggat ccaaaagcta cccaaatact
       61 atagctaacc ccccgctacc ttcaattttg cagataaaac gctttgggag aaaatttcaa
      121 agacaacagg ccatcctatc atgcccgatt ataaacaaaa agctggctat tccttttgct
      181 ctgacaaatt tcaattggga aagttcacga aactagcagg tgaagaagtt tcaactgttc
      241 ggatcaaaga atgcccgctt caaatagaaa cattggttca acgaatttca actagacaag
      301 actttgctat tgtagaacgt gagatcgtag ctatttttgt agaagaaggg attttacatg
      361 atccaactca tatcaatgtg gataaatggc aacctcttat ctataaattc agagaatata
      421 caactgccaa ccagtcgtta actttgaatt ttagattcca agaattcaaa gagtcataat
      481 gataaacatc gttaggtata aataaaaaag actcgcagca cgagtaaaaa acaaatagaa
      541 gctatacatt agaaaggtcg actgtgaccc tatagatgca gtatatgcag cttatgagca
      601 tttcttctgt tctctttgtt tttgggatct gtgctgaagt ctttttcgtt ttttgccgct
      661 ctttctttga aaaatcgatt atgttatacc ttctccatta ctaattcagg catctgctga
      721 gtaatgattt ttcgcgcaaa agaaatatct gttacccaag gttcgatttg ttcttcagtc
      781 aagacaagcg gcattcggtc atggattggt gcgactgtgc ggttaggttc agtagtcatt
      841 atgattgatt ctgccatttt ttcatcctga tggctacgat aaaaaccgcc aatatacact
      901 attttttcct tatttgaaaa cataaatttg tctttttctt cattccactc attgggaaaa
      961 cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact gtctctgcac
     1021 gggcattaat catcaattgt ccttttttaa atccggtaaa tccccacttc atgatttccg
     1081 gagtgaggat gttttcttcc tcttttctta acgtcacaac aagatttgac ggaaaaactt
     1141 ctccagctgc taattctgct gcctgcttcg gttgtttcgt ctgggcttct cgataataat
     1201 tctgaaggag ctctgactct agatcaaaaa aataacgacc acacatccta atccctccca
     1261 atcttttttt ctatcataca ctgcaacaag aagttcgcac caacaaaacg ctttcaattt
     1321 ttgtagaact aaaaaagttg tgacaagtga caaactctcc agtgaatggt attctcctgc
     1381 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgctt tttgaaattg
     1441 ttccttgtga ctcggagtga aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa
     1501 gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa
     1561 tttttcttaa tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca
     1621 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagcag ctatttttat
     1681 ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcca ctaaacggtg
     1741 ttcaatcagc taaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg
     1801 ccgtttgttt ctctaatact caggtcaaag cgctgatttc acaacctcta atacacggtg
     1861 ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaatttg agaagcaatt
     1921 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct ctaattatta
     1981 aagaaccttt tgcgaagtta acaatctttc tttgatcaat tgaaaattct gcctaagtag
     2041 tgatagatcc tgtgtgactt tcaacaataa gatacttccc tggatttcag caatcaacgc
     2101 tttggcttga cggtctgctt cttcatcgga cagtttttct tcagaaagtg ccaggatatt
     2161 ttgtttcgta tacttgaccc attgattgac taattctagt agcaacagac ggtattcttc
     2221 atccactgta ctcaactcaa taatcaaatt acctacagga cagccatgat aagtgatctg
     2281 tgtctcatga tcagcgaaaa tccagtccaa catttcttct aatgcttctt ctggctgttt
     2341 tccagcattt aagatgtctt cgaacaattc tgtctgccat acgtccatat gcgcatggat
     2401 caccgctaga caaacttctt tttttgaaga aaagtaataa taaaattgac cttttcctgt
     2461 atcagaagct gctagaatat ctcggattga cgtttcatga tagccttttt tataaataag
     2521 atctttggtc gcttccaaga tcttttcctt tgttttcaat gatattctcc tttttcattt
     2581 ctcatagtcc tatattatac agtaaattca tcattttttc ttcttaatcg tttaaaaaag
     2641 actgtgccaa attatctgac acagccctca ctataaaact gttctcatat agctagatgt
     2701 tcattgattc aagaactgcg aggacaaaag caattcctat cttatgatag ttgctctaat
     2761 acttcctctg ttgtcatcac ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc
     2821 attgcttctt ggccaggagc accaattgca tctgtcgcta ccacaacatt gaatccactc
     2881 tctaccaatt cacgcatatg ggaatcgcta cacaagtttc cgttcattcc accaagaatt
     2941 actgtgtcga ttccattttt tctcaattgg agcgccaaat cattgctttc tggtccaaag
     3001 attttatgag gagagctgat gatcgtattt tcatccaaat aaggtttcaa ttcttcgata
     3061 aaatcggctc ctgaaccttc ttccactgat tcatattgac tttttctagc aaacatctgc
     3121 tgctcaagca tcatatgttc tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa
     3181 aaataatgag gagaaataaa gagtttgtat cctttttctt ttgcttgttt taccatttca
     3241 atcatatttt cgatcgtatt gtatttttct aagatatctt ttgtcaaacc aaagccaccg
     3301 cctgctggtt tcaagaactc gacttgcggg tcagtgagaa ctactgctgt atttttatta
     3361 ttgaacatgg tggattctcc attcgttatt tattttaata catcatttag agcttcactc
     3421 attgtcggat gagaatagat ttgatctctc aataacgtat aaggaagttt tccattcata
     3481 gctagcgaaa tcaagttgat gacttcatga gaatcttccg catagatact tgcccctaga
     3541 atcaagtcag tttcagggtc gactaaaact ttgaatacac ctttaggatc ttctaatact
     3601 tgtgctttag ggacaccgct tgtcatatac ttgaataatt tgtaattgac gccttgtgct
     3661 ttggcttgtt tttctgttaa acctacattg gataaagtcg gagtgataaa tacactgtaa
     3721 ggcacagctt tccggttatt cgttgttcgt ttattctcgc ccttcaactg atccataatg
     3781 attcgataat catctagaga gatataagtg aactgcaatc cgcctttgac gtctccaatc
     3841 gcccaaatgt tgtctgcatc tgtacgaagg tattcatcca cagcgattgc tccgcgatct
     3901 gttagtttaa catctgtatt ttctaatcca agtgatgctg tacttggttt tcttccagtt
     3961 gcagccaaaa cttttgacgc ttggattgtt acttcttggc catcttctgt aaaggtgacg
     4021 gcagctgttg attcttggtc tgctacttga tctacagata cacctagatg gaattcgatt
     4081 cccgcatttg tcatgtcttc taaaatcatt tgcgcgatat cttcatcttc acgtggaaga
     4141 aattcagaat gggcatcaag aacgacaact ttagatccgt attcattgaa cattgatgca
     4201 aattccaagc caatatatcc agccccgatg atcactaatt tttccggtaa ttgttcctga
     4261 tccatcgctt gcgtagaatc aatcaagtag cgacttgtat tcaatccttt gattggtaaa
     4321 atgattggtt cagaaccagt attgatgaaa atccgttcgc cttcaacaat cactttttca
     4381 ccattattcg tcacttcgat ttgatggtta gaaagaaaac gtgctgtacc gtccatcact
     4441 gtcccagttg cctcatcgct gatcatatga taatttttgg cgcgaagcat gcccgtcaat
     4501 ttttctttcc gagcaacagc ctctgtaaag tcgattcctc tttctccatt gaaaatcaat
     4561 gatttagaag gaatacagcc gatgttgata catgttccgc catacatacg tgttgacttt
     4621 tcaacgacta aaaccgattc accttttgaa gctaacgcct tcgctaaagt cttaccgccc
     4681 tttccaaaac cgatcacaat attttgatat ttttccatca tttttccctc gcttcttttt
     4741 tacctaccga ttggtacaaa aatatgataa ccgataattg tctattctcc aaatcatctg
     4801 ctttatttgt caaaaaaaac cagacaaaaa gcggtccttt cacgttttta gtctggttcg
     4861 atttccttta ttcttctaaa caatcgttac ttctacatcg atatttcctc tagttgcttt
     4921 ggagtaaggg cagaattcat gcgcatcttc tgcaattttt tgggcttctt cttcgctgat
     4981 gccatcgata tgcacttcta atactacacc gatctgatag ccgccatcat ctaaattata
     5041 aagagagaca cgtgctttta ctgtggactc tgctgtcatt cctgcttctt tgatcatatg
     5101 ttgtaccgcc ccattgaaac aagaagcata acctgctgca aataattgtt ctggatttgt
     5161 tccttctgca tggattcctg gaggagtgat tttcatatgc atcgagccat ttggtgcctc
     5221 gacttctcca ttgcgcccgc catggttgat cattgctgtt tcgtatgctt ttttcattgt
     5281 actgctcctt tcgattgacc atttgctact tggctataat tatagtgtat gacaagtaag
     5341 gcgctttcta caaaaattct gcttattcga ataccagaat gccgtcttct actatttttc
     5401 caaaaaacaa aaaaatacta ctcgtgaaca acagcgtcat atttgttcca aaataaatag
     5461 caatcttttc tacataatct tttatatgat tgattttaag atacatatat accagaaagt
     5521 tgaccccata aaccaatact gttaaaaaaa taaccatatt cgttaccatt ttttcctcct
     5581 tttgctggtc tccctaccag cactcgattt aaccatacca ctttttttgc cgtccaagca
     5641 actagccttt caaaaagact ggtttattct gctgttaatg attcaagatc agaaaaatgt
     5701 ccgaataatt ttgctaattt tactaacgcc aacttgttct ttccgaattc atcattttct
     5761 atctgtaata ctagtaacgg ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg
     5821 tatggctctt ctagatcata tcgaatacct tcttcatttt ccgggtcgaa gttcatccct
     5881 ttttcttctt ccaacagtcg cttcatttct tctaatatct tgtttccttg ttcgcgtggt
     5941 tcatcgctga acaattttaa tcggatttct tgtacttcta cgggctgttt caactctttg
     6001 atttgttcat ctaatgtgat accttttctt tttaattctg gcaaaagcat caggatcttt
     6061 gcgatgacat atgcaccatc atccagaaaa tagttttcct tgaatgcagc gtgtccgctt
     6121 gtttcgatgg ctagctgtgt atctttccca agacggttaa gttctaatgc tttattgatt
     6181 acatttcgat agccggaaat ataacgaact tggatgcctc ctttttcttc aatgaatctt
     6241 tgtagatgag aagaagtagg ggaattcgtc acgattcttg ttcctggatg ctcttttaat
     6301 acgattgtac ttaatactgc gatcaaatta tttcgattga tcactgttcc agaagcactg
     6361 acaactgcag aacgatccac atctgtatcg aaaatgatcc caagatctgc ttcatttgct
     6421 aaaacagcct gcttgatact ttccatcgct tctttattat caggatttgg gatatgatta
     6481 ggaaatcggc catctggttc caaaaattgg gaacctgttg aatccgcacc taagctattc
     6541 aatacttttt cagcaaagaa tccaccagca ccatttccgg catcaacaat gattttccaa
     6601 ccatttagtg gtttgctttc tagtgttcca atcccctttt taatttttgt gactaaatct
     6661 tgcgcataag gtgtcaaaag gtctgctttt tctacgcttc cagtttgttc gctttttatt
     6721 ttttgctgct gtgataggat aaaagcaata tcttcttttt ctgctcctcc ttctgaggta
     6781 aagaatttta ttccattata ataataggga agatggctag cagtcagcat aaccgcagca
     6841 tcgcacgaga actgttcaaa ttgagtggac ataaacatag caggcgtagt agctaattcg
     6901 aaatcgatga catgtatccc ttgttctttc agactttgga tcaacgcctc tttcagtgct
     6961 ggtccgctta tcctactgtc atgaccaatc ccaatcgtcc attttttatt tggatcggtt
     7021 gatttcttct ttttctttat ccagtttgct agtccccagc cgatcttttt taccatgtct
     7081 gtcgtcaagt ttacttcata tccttcaact ggtaatgcga ttcctcgaat atccgatcca
     7141 ttttgcagcg ctttcatatc tttcatataa aagtcctcct catttattcc ctcatttttt
     7201 ccagcttttc tgcaaatcgc tgtgcttctt cttctgagcg gcaaataata atgaatgtat
     7261 ctacaccggc catcgttccg acaacttcag ggtaagccac ttcatctagc caattcgtta
     7321 ccacatcggc accatttcgt tcagtcaata cgataaccat gaactgaaca gactggatac
     7381 gcaaaacctc gcgtctaatt gctgtacgca aacgttcttc atttaaagaa acagatggct
     7441 ggttaaataa tgtatagcgt actattttgt tttcattttg actttttact aacttcattt
     7501 ctcgaatgtc tcgagaaatc gttgcttgag tcacatcggc gcctgcttca tgcagccgcg
     7561 caagcagctc ttcctgtgtc ccaataggtt gttctgtaat gatctggcgg atcagtgctt
     7621 gtcgctgttt ctttttcata ttagacacct ctcaatgttg attatagcaa aagataagta
     7681 atatttcttc atattttggt attttttact tttatttttt gttttcattc aggttgctaa
     7741 cctttttcta ttttaacacg caaaatcgtc ttcatttttt caggtgctga ttttcttgga
     7801 tcgctgatgt aaatctcttt gtgttcttta gaagctcttt catagccttc ttttgcgatg
     7861 aatgcttcta atttctcaaa cgttgctggt tcttcggcat agggcccgac gtgtaagatt
     7921 tgggcacaaa gaccttcttc catctttttg aatactactt gttctttcaa gtcttccgga
     7981 agtttgctag aacgctgaat ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt
     8041 cggatcatca attgataaga aaaatgttct tttttcatca ctggttcatg caaatacttt
     8101 tcttgcagtc cccattgtcc ttccagtgga tagacagtat acatctgata atcaggaatc
     8161 agccaatttt ttttaggcga catgcgtatt gcatagctga ctgcatatag acaggctact
     8221 cttcgtttaa agtcttcctg attcgggtcg ccgatccctc tgatgctaaa gaatgatttt
     8281 ttaggaacct ctagtatttg cgggcttttt ttcatactat agctcttgtc tttacgccat
     8341 tcaatttttt ccatttttct tcctccgcga aattccattt agttatttat tatcatacct
     8401 ctttttgagt gacttggcta atcgtgttga cactattttt cagtttgtgc tagaataagt
     8461 gaagtaaata aatcgtacag ataacacgtt tcggcgtgtc gagagagagg cggtttctat
     8521 ttttataata gaaatcactc tcttttttta cgaaattttt tagaggtgat gtaaatgaca
     8581 aaacaaaaac agctgctttt agtacctgca gtaattgcaa cagggatgat gtcttttgcg
     8641 ggcgtattga tcgagacggc catgaatgta actttcccaa ctcttatcag acagtttgat
     8701 ttaacgactg cacaagtcca atgggtaaca acgatttatc ttttgatgat ttcgatcatt
     8761 gttccgctgt ctacttattt gaatcgcaat tttagtctca aaaaactctt tttagcttca
     8821 aatctgcttt tcttggcagg tataatgatc gatttcttct ctcctacctt catggtccta
     8881 ttgtttggtc gattgctcca aggtgcagcg acaggtatcg ctcttccgtt gatgttccat
     8941 attatcttga catttgcccc cctcaatcga cgaggcgcga tgatgggtgt cggtactttg
     9001 acaacggcca ttgctccagc aattggcccc acttatggag gcattatgac ttcacatttt
     9061 acatggaatc atatcttcct gtttcttgtg cccgttttgt ttctttcttt gattatcggc
     9121 tggtttgcta ttcccgagat ggcagtacaa aaaagtggtt atttggattg gataagcgtt
     9181 ttaggaattt gtcttttgtt tagtggaagt ctaacatttt tgagtacgtt gggaacagtt
     9241 tccagctgga ttgctttaat catcggcggt attggctggt tcctttttta ctatcgaagt
     9301 aaaaaagtgg agcatccact gatccgactg tctattcttt caaataaaac attccgcttg
     9361 tttttgattg gcttcttagt ttatcagttt ttattacttg gtgtatcttt tgtcttgccg
     9421 aatttccttc agatcgttca aggaagtagt ccttttgtcg ctggattagc gatgcttccc
     9481 ggcgcagcgg ttggtgcttt gctttctcct ttttccggga aaatgctgga tcaatatggt
     9541 ccgaagaagc caatttttgc tgggctgatt ctttctttga ttggttggct agcattattc
     9601 ctactcatag gacacgcttc agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc
     9661 ggtatcggat tatcttacag caatatgatg accaccggaa tgaatgcact gaatgaagaa
     9721 ctacaaggag acgggaacgc aatgttcaat accttgcagc agttttctgg cgctgtggct
     9781 acttcattag tagcggtcat tatcaattac gtccagcatc atacttccca taactatgaa
     9841 gtgtctacta cattaggatc aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta
     9901 agctttgctc gattcgccta ttatctgttc tttgctaaaa aagcgtagat tccatatacg
     9961 gcaggtgttg catctgccgt ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt
    10021 ttatggaaaa aattgccatt atcggtggcg ggatcattgg tatgacccta gccagttact
    10081 tagatacatc aaaattcaat gttgctttat ttgatgaagg gacaggacag gcaactaaag
    10141 ctagcgctgg gattatctcc ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc
    10201 tggctaaaga cggcgctgct tttttcccta aacttataaa agatttccaa cttgatcaaa
    10261 cgatttacgc acaatcaggg acacttttgc ttcgacctga aaaagattta gctgaattgg
    10321 ctgttcttgc cgagaaacga aaagaagaag cgccagaaat tggaaacatc gaatggcttt
    10381 cacctgaaca gacatctcaa gcacttccgt tattgaaacc agcagtttca ctgcgtatca
    10441 gtggtggtgg tcgtctggat ggaaaagcat atttagaaca tcttggtcag cgtttagcac
    10501 aaaaacaaat ggagcagatc caggcaaaag tcactttgca taagaagagc gattcttggc
    10561 tcatcaaggg accgtttgga gaatacacag ctgatcgtgt aattttgaca cctggcccgg
    10621 cacttaagcc tttattacaa gaattaggct atgctgcaga tatacgtccg caaaaagggc
    10681 agctcttagc atttcagaca tcttataaga tgagtcaaga ctggcctgtt gctatgcttg
    10741 acggagaagc tgatttcatt cctttcatgg atggaaagat ccttttaggt gctacccatg
    10801 aaaacgatca aggctgggat ttggctccta ctagcgaagc ttataaggga ttgactaaga
    10861 gtacagaaca gttcttgact gatccagatc agataaaaaa ctggccatac gagttccgtg
    10921 taggtacccg tgcttatacg tcagattttt ccccattttt cggcgtactg ccagatgatc
    10981 caagcttact ggctgctagc ggtctaggct cttctggctt gaccacaggt ccttatatcg
    11041 gctatctatt agccaacttc ttaaatactg gtcattggcc tacggaagat tatcaaaagg
    11101 acatctccac ctatattacg aaaaatccat catagaaaaa cagctttttc aagtaaggat
    11161 cccgagcgat attgcgcatg gattacttct tgaaaaagct gttattttag ttcaatttct
    11221 gaatcaaatt ctccaaactt tcttttgctt catctggcaa gagatctttt cctatctgag
    11281 cttcaatgaa tcgtaaacga atatcaagtc gttgtttcac cagattttta taggacgtat
    11341 cctcaaaaga aacaggaaaa tctggtatag gtaaatagtg gatgtcttca aatggaccaa
    11401 acgacttgaa ttctgccgtt tgttgcacga ttctttcaac gattgacaat gtcgtttcag
    11461 gtttgatttt tttatcatcg aaatatccag tattcaggac atagcaggct gtttgctgtt
    11521 tcaaaaacaa ctctcgaaaa gcttgatagt cttccctaag aggatagaca cggaaaggat
    11581 tagcaaatgg ttcaatgact agctcatcta gctgtctttt tgtagtgata ttttccgcgt
    11641 ctgatcgttt tgttgctaat gttgcaccaa aagacgctgc taaaactggg tcattgatct
    11701 tgataattgg cggaagcgaa tcatctttca tgatccaaaa aacagcatcg atcttttcgc
    11761 taagatgatc gactcggtcg ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt
    11821 ttcgtatatc tcctgttact aggacttttt ctcctttact tgtacatgtc acgcccacat
    11881 tctgacaagt aaggaaagaa gaaatggccg gatcatcaga cggatagtct tgtgttttat
    11941 caaagtaaga aggttccaag gcagtagcaa aaccattttt tcgagaaata acgaaagcat
    12001 cgtcatgcaa gatcgaaaca gagtgacctt ccgtatgttt tgctaaggta atcgttgatt
    12061 ttccagaacc ggacaaccca aatacagcca tcgtataggt ctggtcatta ctagcatatt
    12121 gtttcatccc accgtggcag gcaataaagc cattgcgatt tcccaatgcc caagcaagcg
    12181 tcaaagtcgc tttttttaat tctccaaagt aacgtaatcc tagcacagcc gctgtattat
    12241 ggagagcatc aaacaaaatg agtccatttg gaaatgtttc atgtgtccat tcgggatcac
    12301 aaaagatata aagatctccc tcatcaaagg aaacagactt ttgataagtc tctaagatct
    12361 tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa attgttctca aatccttctg
    12421 gaatcagcaa atgactttgc acagaaaatt cttcagatag gccaacgatt acttttgtat
    12481 ggtaaaactg gcgcttgcga ctttgataca cagcttctct taaaattttc tcgtatttgc
    12541 tttgttcctt gtctaagtcc tcaaagatcc gccgagcact tgctgttctt cccacaatgc
    12601 ttccatcatt gaatactagt gtcctaacat cagctggtaa atctagttct tcagcatgtg
    12661 agattgttaa atctgtgatg atcgttcctg gactcccttt tgctagttga taagctgtct
    12721 tcctatcaaa aacttcatga acattattac cgtaaaatgt cgtttctaca atcgatcgaa
    12781 tactggataa cagtggattt ccacttgtga tttcgtttaa ttcgagactc tcttttgtac
    12841 tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc tttattatac attattcgag
    12901 tttttatttg tactgctgcg ctgtgggaag caaacgagtt atttttcaca caaaaaaacc
    12961 gagaacttga ataaacaagt ttctcggttt catcattttc cttttatcgg cttagtacca
    13021 gccgtttgct aaccagaatg ttttagcagc atcccatgaa ccataacgag atgttacata
    13081 ttgttctgct acacgttctt gattggcagc tgaatagtcg ccgtttaagt atgaagcatc
    13141 taattgataa cggccgatgt aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt
    13201 ttgtgcgatc cattcttttg cagagcttga agttggcgct gttgcagcag ctggggcagc
    13261 aggagctact tcaactgttt caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc
    13321 agttgtagat gctgtatttt ctgttgtttt gttttctgtt gttgctgaag aatcatttga
    13381 agttgggatt gttaattgtt caccaacata aatgcggttg atgtttgcga tcgaattttt
    13441 ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa
    13501 tgaatcacct gattttactg tataaacttc atcagcgtga gcatttgtag ttcctacaaa
    13561 cattgcagta gcaccagcag caaaagttgt acctaaaaca attttcttaa atgaattcat
    13621 attaaactcc tttttttctc cgttttttat tctttcgacg ctattaactt
//
LOCUS       DAYYFD010000076        13566 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000076 DAYYFD010000000
VERSION     DAYYFD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.076"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1666
                     /locus_tag="ACHA25_002154"
     CDS             29..1666
                     /locus_tag="ACHA25_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763436.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1811..2551)
                     /gene="truA"
                     /locus_tag="ACHA25_002155"
     CDS             complement(1811..2551)
                     /gene="truA"
                     /locus_tag="ACHA25_002155"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8763437.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2742..3377)
                     /locus_tag="ACHA25_002156"
     CDS             complement(2742..3377)
                     /locus_tag="ACHA25_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8763438.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3524..4744)
                     /gene="sstT"
                     /locus_tag="ACHA25_002157"
     CDS             complement(3524..4744)
                     /gene="sstT"
                     /locus_tag="ACHA25_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8763439.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4963..5961
                     /locus_tag="ACHA25_002158"
     CDS             4963..5961
                     /locus_tag="ACHA25_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763440.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6157..7992
                     /locus_tag="ACHA25_002159"
     CDS             6157..7992
                     /locus_tag="ACHA25_002159"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8763441.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8052..8768)
                     /locus_tag="ACHA25_002160"
     CDS             complement(8052..8768)
                     /locus_tag="ACHA25_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8763442.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8995..9537)
                     /locus_tag="ACHA25_002161"
     CDS             complement(8995..9537)
                     /locus_tag="ACHA25_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8763443.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9542..9769)
                     /locus_tag="ACHA25_002162"
     CDS             complement(9542..9769)
                     /locus_tag="ACHA25_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8763444.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9848..11728)
                     /locus_tag="ACHA25_002163"
     CDS             complement(9848..11728)
                     /locus_tag="ACHA25_002163"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8763445.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11917..12531)
                     /locus_tag="ACHA25_002164"
     CDS             complement(11917..12531)
                     /locus_tag="ACHA25_002164"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8763446.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12673..13233
                     /locus_tag="ACHA25_002165"
     CDS             12673..13233
                     /locus_tag="ACHA25_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8763447.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         4250 a         2635 c         2139 g         4542 t
ORIGIN      
        1 ttttattact tttaaatagg agaaaaaaat gaaaaaaaac aaaaaccaaa taatagatat
       61 catttttatg ttatttctaa gttttgctta cgtcatccct ctattcatgt ctaagggaat
      121 ctatcatagt gtcaatcagg atacttactt tcatttatct cgaatcatag gactagataa
      181 tgtatggagt tctccagtaa acttcaataa cttcgatcat catggaacaa tgatgaatat
      241 tttttatcca tggcttactc tttatccagc ttttttattt tataaaatga tgggaaatct
      301 tgttttaagt tataatatct actatttttt tatcactttt ttaacaatgg tcgtttctta
      361 tttttctatg aaacaaataa aaaacaatcg ttatatttcg ctgttatttt cgattattta
      421 tacattttcc gcctatcgtg ccattgacat attccatcgt gcttcattgg gagaagctgt
      481 cgctttaaca tttttgcctt tgatactaat ggggtgttac gaaatttata ttagagatta
      541 ccaaaaatgg tactggttgt ctattggcat gacgctagta gtgtatactc atttactttc
      601 tgtcgctatg gtttctgtat ttattggagg aacgttgttt ctttcttttt atttttggga
      661 ccaaaaaatc gctagactct taagtctttt aaaagctacc gtcctcacct tttttttaag
      721 tgcgggattc ttaattcctt ttattcaaca atctagagca caagaattga aagtcccact
      781 gggaaaagaa ttaagtggta tggctcctag tgatatgctg acacatattt taaataataa
      841 ctataacaac tatactattg gattattctt atttctcgga ttgattggtg cttttatttt
      901 tataaaaaaa ttaactgctg atgatttatt tattttcttt cttggtgtat ttgttctttt
      961 ttgttcaacc aatttatttc cttggcaact ttttaaccat acaccagtta agagtctaca
     1021 atttgtctgg cggttaaatg gtttttcttc tttatttata gcctatacga tgagtatagt
     1081 gatctattat attttttcta ctaaaaataa ttcatggaaa ataatggtct ttacgtttct
     1141 tgcactaata ttgcatctct caggagtaag caattctatc catgctaaca aagattcatt
     1201 aacacttata gcacctgaag acgctgttgc tatagctgaa aattacaatc atactgatta
     1261 tgcaaacaaa gagtcaatct atcatcctga tatgattaat aatgatcaat atttactagg
     1321 taaccaggaa ataaatccaa caactcattt tatgagtaac aaacttacaa ttgaattaga
     1381 taattcgaat aataaaaata ctgtcttgac gacacctatt tatcgttata aagggcaagt
     1441 ggcttctatt aatggcaaac tagtacaaac aaaattatct aagtttggca caactgaatt
     1501 aactattcct ccaggtataa ataaagtcgt tattacttat cagtacacaa aactagcaat
     1561 tgcttcaaga tatttatcta tcgttacttt gattttattt ttactttatc gatttacttt
     1621 ttctaaacaa aaacaaccga gaagagaagt acaacattct cactagattt ttatttaaga
     1681 aagacttttt agctgtacac ataaacaggc taaaaagtct ttttttatat ttctttaatt
     1741 tgcttcatac attatttaac atcgatattt atactgtgaa acatgcgttt catccattat
     1801 caaagtattt ttatcgataa taaacttcat ccaaaaacag accttgtgca gggacggttt
     1861 ctcctgcatg ttgccgcact ttgctttcaa atatttcatc gattgtttct atcggcatgg
     1921 cacctgtgcc gatttctagt aatgtcccca tgataatccg caccatttta tataagaatc
     1981 cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat aattcatcaa
     2041 tcgtacgcac tgtggatttt tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg
     2101 tacccactaa tttttcgcaa gctgcattca gcttgtccat atccaactct tgagggaaat
     2161 aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt gttccaaaca taatagctgt
     2221 attttttgcc caccgcatta tatcttgaat ggaagcgctc aggcatttct tcgattcttt
     2281 tgatgacaat atcccgcgga aggtaacgat tcataaagtc gatcatttct gccagtccaa
     2341 tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca gcatctgttc
     2401 ttcctgatcc aataatttct atctcttcat ttgtcatctg atgcaatact ttttcgattt
     2461 ttccttgaat ggttttatcg tcatctccaa gtctttgcca tcctgaatat ctttttccgt
     2521 catattcgat tgtcaacttg atgtttctca tattttagtg gctcctctgt cttccatttg
     2581 gtcttctctc taccttttta agattgtagc acaaaatata gctccttgct tctctaagac
     2641 agaagctata caagaaaact gtggaaatat atatttcaga aaaacaaaaa acagccaccg
     2701 gatgaagtat ccgctgactg ttcaatttga cggctcaagt tttactgttt gagatgcaag
     2761 atactcaacc aggcaatggg cacataatta aagaaaaaac tgattctttg gcaaaaacct
     2821 attttattca gtaatggcca agaatttata gaaggaattc ttgccagtcc ataaatcgcc
     2881 gcaaaaagca aactgatcac agacagtcca aggtataagt ggcttacgtc catttgccca
     2941 ttttttcgat agatcaagaa aagaagaact ggaaacaata aaaaaccact gtaacctaaa
     3001 cctgatccga tattatggac ccatgtcgag acattccacg aagattcatg ggtattgata
     3061 ctgaacaaac ctgtaaagat acaatcacct ataccataga acgcgatggc tgctgtaact
     3121 agggtagcta acacacgaga tgtactaaca aatgcagcat acaatgcagg caatgacagg
     3181 atgaaaaaga tccctgaaac cacagaccaa ataagaaacg cttttcttac aggactggaa
     3241 acctctccaa atacactgat cacgctcttc atttgatcta attcaggata atacaatcct
     3301 aatacgtacg gagtcaaaaa atcactcagc acgcctaata ataaaaagaa aaaaccaaat
     3361 ttacgtaaaa aatccatgat acgcctccaa taacaattac ctatgctttt aattgttatt
     3421 ataaacgact ttatcttgat ttaacagaaa cctctattta cacgaacatc tatataactc
     3481 attaaaaact cgaatcattt taaaaatact aaatatctaa cgttcaacgt tcacttaatc
     3541 catctaaaca atctttcaat ttgatttttt ccccacttct tttacgcgcg cctagctctc
     3601 ctattgcagt aaacaacaga tcacttgacg aatttaaggc agtttccatt gaatcctgga
     3661 taacaccgat aataaatcca ataccgacga tttgcatcgc gacatcatta gagataccaa
     3721 acaaactaca tgctaatgga atcaataaca gtgaaccgcc cgcaacacct gaagctccac
     3781 aagcagctaa agcagacaaa atacataaaa ggactttcaa aacaaaaggt gcttcaatcc
     3841 ctaaagaatg tacagcagtc aacgtcatca aggaaatggt aatcgcggca ccgcccatat
     3901 tgatcgtagc acctaacggg atcgatatgg aataagactc ttttgtcaat ttcaatcttt
     3961 cagccaacat catgtttatc gggatatttg cggcagaact tcgggtaaag aaagcgggaa
     4021 gtgcgctttc tttcaaacaa aagaaaatca atggataggg attttcttta ataaacaaaa
     4081 acgtgaggaa tgggtacaca actaatgcga caaacagcat cgtaccgatc aaaacaagta
     4141 aaagctgcag atactctgcc aatccagcga ttccagtcgt agcaattgat tgataaacca
     4201 atcctaaaat ccctatcgga gcaaatgcaa taatcacttg tacgacttgt gacaacgcta
     4261 tagatatctg atccaccaac tcttttgtcg attctgaacg tgctcgaaaa gctagtccca
     4321 acaatacagc ccagaataag acgccaaggt aattcgcatc aataagagat tgaacaggat
     4381 tttgtgtgac attcgtcaac atagtagata aaacactttc caaactttgc ggtgcttttt
     4441 cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca ataactgcta
     4501 caagtgcaga aagcaatgtt cccactaaat ataagatcaa aataggcttc acgaatgtct
     4561 tattcccaat tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg
     4621 cgattgcctt caagcaactg acaaacaacg ttcccaatat tgaaataaat gtccatgtgg
     4681 ggaaaaagat accaaacaat attccgacta aaataccaac aacaatacgt tgaactaacg
     4741 acatatccaa aaacttctct aacaaatgac caccctttca caattttaac aatcattgta
     4801 tcattttatg caaagaaact aaaggtataa ttttctaaaa attctaacaa tacaaacaac
     4861 catttcttgc taattttgta ctttttctgt ctacacgttg ttttttgaga tattctaagt
     4921 taggatgtta ttaatgggca acttatcagg aaacgttttc ttatgttttt gtccaatttt
     4981 tttgtgaggt gtttattctt gaaaaaaata aagaaggaga gttatggctt cctgacaatc
     5041 tttgtcgctt gtataatgat ggttttacag cgtatattga tttcagcagt cactattcct
     5101 acatatgcaa caaaccctgg tcgattctac atctatacat ttttacaaac ttccgttgtt
     5161 attggtgcaa atttgatccc actttacatg ggttaccaat atcaaaaaat cgcccgttta
     5221 aaagttttac attatttaag tcgttttgcc tttatattcc taatagccac actagtttgc
     5281 aatatctttt tttacgtaca agctggcagc ttgaatattc gtgattattg gcttatccta
     5341 acacctatca gtcagaatta ctttacttat gctgtttctt gtactttgct tttttgtagt
     5401 attccttata cagttggatt tttaaataaa ttatcgaaaa caacaattaa atatctacta
     5461 ttatttttaa caattggatt agttggctgc tctactctat ttaataaaga cccgtttgca
     5521 ttacagaatg gacagagtat tttatggatt ttttatttat ttttaatggg ttatggtttg
     5581 aaagaatgga actggaaaga aaaggtccgc tataagcttc ctcaagtatt tatcagcttg
     5641 attgttttat tcggtttgat tattttgatg acacaaattt cactcatcat cagaggaaat
     5701 acagatacag cgctgaggtt ttctaaacca tattctctct tcgcaatgta ttatactttc
     5761 tcttcgtttt tatttttaga aatgctatct gataaattag gtataaaagg aagaggaaat
     5821 tttcttgctg tatttttgat tgtcactcaa gttatatgta atttcccttt agtttcttat
     5881 cgcgtatcta catttatgaa acgtgaattt ccaaactcag ggaaggcatg gctgataaat
     5941 atcgggcagt tttttatttg atatttatta gcagtgtccc tcctagttat ctttttattt
     6001 tatatacaaa aaatccgttt cattcatcgt ttcatcgaat acttcgcttt tgattcgatt
     6061 gaacaattaa ctcagcgttg gaaaaaaaga attcattggc tttttgttca taaaagttat
     6121 tttttagttg cagcattttt ttactgcttt accttcgtgc agattttcgt tttagaacca
     6181 aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa tatttactca gcgacaagca
     6241 cccatgatcc tgacaatcat cattattatg ggatttttct tcttactatt gttgatttcc
     6301 aatcgttttt ggtacgcctt gactgctacc ttgatcatta acttactgct cactatttcg
     6361 acagtaatca aaatggaaat gcgggaagag cctgtctttc cttcagattt aaaaatgctg
     6421 accggattgt cagaactatt atcgatggtt agcccagtct tattaatcgt gggcggattg
     6481 atccttctcc tattattgat tactagtatt attgtacaac gtagactaca aaaacagtat
     6541 tccttgaaga ttcattggaa acgtcgattt atcagtatcg ctgtattatt aggatgtttt
     6601 tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt tcttgctgtt taatttattt
     6661 aaagtaaaca agactttctt taaccagcaa ggcgccgtaa aagataacgg accagtcatt
     6721 caattcctaa acaatatcga cattaaaatc atggaagaac ctgctggcta ttcgaaagaa
     6781 aaaattaatg gaataatgaa aaagtacaac acaaaggcag atgaaatcaa ccaaacaaga
     6841 agtgactggt taaacaatca gacgatcgtt ctcaatttaa gtgaaagctt cagtgatcct
     6901 gcacgtgtcc ctaatctgac agtacctaca gaccctattc cttacatcaa aagcattata
     6961 acagaaggaa caggaggatt gatgttatcc gtcggttatg gtggcggaac agctaacatg
     7021 gaatgggaag gactgacagg cttgagtatc agtaacctgt ctgcttcgtt agttacacct
     7081 tatacccagc tagtggagcg tcagaaaata agtccaaata ttaccgatct attcgatgaa
     7141 aaaatagcca tccatccttt tacagctgct ctttatcgca gaaaacaagt gtttgataaa
     7201 tttggttttg acaggttcta ttatttagac agtccggata aattaactta tacggataaa
     7261 atagaacaaa gcccacgcat atcggatgat tcagcataca aagaaacctt gaaagcttta
     7321 caaagcaata cggaaacgtc acaattcatc caattatcca ctatgcaaaa ccatatgcct
     7381 tatgatgatt actatagtga attgaactac actgctgaag gaactgctgt cattcctaat
     7441 agaagaaaag aattacaaac ttatatgcaa ggccttcact ataccgatac agcagtaaaa
     7501 aaattcatcc aagaaatcga caaaatcgat aaaccgatta cctttgtttt ttatggtgac
     7561 catttacccg caatctatag tgggaatgat tcgaaaaaat atggattaga acagcaccag
     7621 acagattatt tcatttataa caacaagtat agtcgtgaac aagcagctac gttaaataaa
     7681 gaagttgttg ctcctaataa ctttggtgct atggctcttg agcatgcgaa tatcaaagta
     7741 actccttact atgcgttgtt gacagaagta tctaatcatc tacctgcatt aacaattgat
     7801 cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg tctttgttac agaggatagt
     7861 caaattatcc aagaaaaaaa tttaactgag aaacaaaaag aaattctaga agattatcgt
     7921 ctgatacaat atgatcttgt tgcaggtaac cagtacagtg caacatgggc gacacaaaaa
     7981 ataaaagact aacaaataaa ctgagcaagg ggttgtgata aaagacatgt cacaacccct
     8041 tgtttatctc attaatttgc ttgtccataa acgatttgat taaagccatt tttcaattca
     8101 ttattcgctg tctcgttgaa gtaaagataa ttatcgggat tcccctgatt ttgttgtgtc
     8161 accaatactt ttggctgatt ttgctgaaac ccgaatagat agacgtgctt tttcagatat
     8221 ggttctgtct ctgcatcgga atagacggct gtgatttgat agtctgcttg acctgtccca
     8281 ctttctgacc attctgcaga aacaggcact ccaccaatag ccatcttcca ctccccgttc
     8341 aaaatagctt gagggacttt aagaccatat aagtccacac tcatttgatt cgtataactt
     8401 ttgtattgtt gtcccattgt ttggccccat ttggtcataa atgcctctaa ttgtgcggat
     8461 ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc tttctgaaga ggaagatgaa
     8521 gcctctacag aagaacttga agtagatgtt tgagttgttt cagtcgtttt ggaagactct
     8581 gacgaagtag aggtatctgc tgtttctgta cttgctgcag ttgacttgct ctctgtgcta
     8641 gttgttgtag tagttgcaac tgttgctttc gctgtttctt ttgttgattc ttttttcgat
     8701 tcattcccgt tggtacaacc agctaacaaa aaagctagac caattgttac taaaatcctt
     8761 tttttcaatt aaaaaactcc tttcgattac tcccacttct ttattctatc aaataagcaa
     8821 tgtgatttta ttgatttttc gtattttctc atcaatcttt caaatcttct aaaaactgag
     8881 aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc ttcttttatt ttaatcttcg
     8941 tgattttcca atttacctca cgctttatat ttgtatttga agaaacatag acttttagtc
     9001 atcatcgtct tcctcatcca aatcttgtcg gacagttttt ccttggagtc tttgtacttg
     9061 gtagatgatt tctttattga aacttaaaag ttcaacaagc aattgttgca agccatattt
     9121 ttcttctttt tcagctaatt tgattgcccg tgtgataaag acattttgga aatcaaaatc
     9181 ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat ttgttttgac catgttcgta
     9241 aatttttcca taacgcatga cttcttctaa agtagacgga acgatttcat tttcaatcac
     9301 taactcatga gagattttat tgaaccatct cgcttcttga tcagcatatt ctgaaaaaag
     9361 ggtacttaat tcttgcgaag atgtacccaa aacatacaat tgtgcttgga tcaatttcat
     9421 ttcttctaat ttaaaatttg ctaatacatg ccccaccatg gcgccagcag ttggtttatg
     9481 atggtcgata tctgcttgtt ttgtttcttg aacaaatttt tcttccacta tattcattga
     9541 tttacgcctc ttttactttt gctgattgga cttcataacc taatttttcc acaactgcag
     9601 ctaattgttc agggtggact tcatctggat caaagtttgc tttgattttg ctcgcattaa
     9661 acaaaacttt cgtattgctt accccaggct gttgagaaac cgcatgttcg attttttgca
     9721 tacagcttgg acaagctagt gtatctaatt gcataataat ttttttcatc gtagttcttc
     9781 cttccttttt cttgatagtc ttattctaga ttacagcttc cataaaaaaa ttgattctta
     9841 tcaagtttta cgatttttct tctttgcttt cgtgtccaaa atacaataaa cgcatcgcgt
     9901 taaagataac gatcaagata ctagcttcgt gaacgaacat cccactagcc atgtaaataa
     9961 acccggcaaa taagccgttg agtaaaaata agacgacacc aatggcaatc actacatttt
    10021 cacgggtatt taaaacagtt ttcttagcca agcggtaggc atgaaccaac gattcaaaat
    10081 tagattgcat caagacaacg tcactcgttt cgatagcaac atccgttcct gatcccatag
    10141 caatcccgat atcagccgca gccaaagagg gagaatcatt gattccatct ccaataaaag
    10201 caacgcgcaa tccttgttct tggtattttt tcacaaattg aaccttttcg tctggtaata
    10261 attcagcatg gacttcatca atgcctaaca tatcagctac ataatcagca gtcatctgat
    10321 tatcacctgt cagcatcact aactgttttg ctcctgcttg tttcaactta gctaattgtt
    10381 cagcgacttc tggtcgaatc acatctgaca cacctatgat ttgagtgatt tttttgtcga
    10441 ttgctacaat aatgaccgag ctgcctgttt gttgtaattg atgtaaatct tgtacttgct
    10501 ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt gttgcccgca caaactgtat
    10561 gaccatcaat ctcagctgtg attccttgtc ccttgattgt ctgattatct ttgactgcta
    10621 attgttgaaa atcaatgtct ttctcctgag cataatggat cacggcgcgc cccagaggat
    10681 gatcggataa cgtttctaat cgagccacta aagctaagct gtccccttca tcctctgtat
    10741 agttcttgat agtggataca gcagtagttc ctttggtcaa tgtaccagtt ttatcaaaga
    10801 caaaagtatc gattttggaa aaagtatcca ttgcttcacc gcctttgact aagatgcccc
    10861 gtttcgctcc attaccgatt cctgctacat tcgaaacagg cgcaccaatg actaaagcgc
    10921 caggacaacc aagaaccaaa acagtgattg ctaaacgaaa atcacgacta atcagaccaa
    10981 caaccaacgc aataagtaaa acggttggag tataatactg agaaaatcga tcaatgaatt
    11041 tttccgcatg ggatttggta tcttgtgctt cttctactaa ttcgataatt ttggcaaagg
    11101 tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta gccatctgct aaaatcgttc
    11161 cagagaagac ttcatctcca ctgattttga cgacttgtcg tgattcacca gtcactgcgg
    11221 cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc cacaggaata gctccaccag
    11281 tttttactaa gacgatatcg ccttcttcca cgtcatcgat ttccacttct tcagtcgttc
    11341 catcttcttg gacaacacta gcagtagatg gggccatctc ggtcaaagcc ttgattgact
    11401 cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc aaacaaaaac aagaatgtaa
    11461 cgatagctga ctccttgtat tctccgatca taaaggcacc aagcacagca atagtaacga
    11521 gtaaatcaat tgagataact ttgactttca ctgcctgata cgcatggatc ataataggta
    11581 tgaccgcaat aatagaagct atggctagag caatgttata gaacaagaca aaaccagtaa
    11641 attttccgat aaaaccaaaa ataatcaaca cagctgataa ggcggtgata tgatttcgat
    11701 gctcttggat ccacttttgt aatttcatct ttctccctct tttcctttca ttcttacgag
    11761 actaagtata gatacttatc catcataaaa aattgaccac tatcaagttt agtcaaaaag
    11821 gcggttttct tttggtagaa tacactacaa aaaatagtta gcaagaagac tcactaaaag
    11881 tgatcttgcc aactattgtc gtttctatcg aaattttcat tgaatgattt tcctgtacag
    11941 catacatcgg agatctattt ctttatctaa gatttttatc ttgctaattt tttcctttag
    12001 tctttcaaat ccacgtcgtt catagaattg atacgtacag ccactatctg taaaaaggaa
    12061 tatttctttt cctttctctc ttttttcaaa ttctgttagc aacttggtac cgatgccttt
    12121 tgttttcaaa tcaggatttg cagctaaaaa aatgatctct ccatctggcg aatgcttctt
    12181 taaatatgct gcaagcattt gttcatttgc tgtctcgtaa gctcctttac tttctttagc
    12241 aaatatattt tgtaaaaaat cgaatatctt aacatatatt tgctgtccaa aagaacgatg
    12301 tttctttttt tctcctttta tctccgccag caaaacacct gccaactcgt cctctgcata
    12361 caaagcaaga atttgagttg ctcgattcaa ctccatatac aaaaaatatc ttccgtaaag
    12421 attcagcaga agttcatttt ccatatatct ctcaaagttc attccttcaa cagcaaaata
    12481 gatagctttt ttgtaatctt tcttttctaa gggtttaaaa gttagtttca tcatttcgcc
    12541 tcctcaactt caatgattgt gttatactta cattataata gacacagtgt ctactatcta
    12601 taatcaatca tctataagtt tgtcgattaa gcgacagctt tgctttgata tgtcggataa
    12661 ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa aaacagaaaa agctttggct
    12721 gaaacactat ccacattatt agtaaataaa aagattcaag caatcactat acgcgaacta
    12781 acagaaacgg cagatgtaca tcgttcgact ttttatacac attataagga tatctatgat
    12841 ctatatgatc aattagaaag caactttttt cgggatctaa atgaaattct ctcttatgat
    12901 ccagcccata gctatgagga actatacact cgcttgattg actatgttta cgcaaatact
    12961 tttttatatc gcttatttat cctcaaccat ggtgatattt cgtttcagaa gaaagttaaa
    13021 caagttattg aagaaaacta tctgaacata tggctatatg aagacaagaa aacgattatc
    13081 acagaagaaa tgagattctt tactacgtac cacgtccaag gttgtctatc cgttctcaat
    13141 cggtgggtag aaagcaatta caagtactcc aaaaaagaat tattagaact cttacagaaa
    13201 ctgaatgatc acatagagaa aatcatgcct tgaataaaaa catactaagc ttagttaaga
    13261 agaaccgtaa ccttctcgta taacaactga atcttattta gccaaattgt ttactgaaga
    13321 ttttttgagt aaaatttcta taattcctac ttattgaaaa gtagtttgta gtgctgcttc
    13381 tccctctcta agtgatatat cttgttgtac caatcaaatt cacctcatca ttaataaatt
    13441 acttttaggc taatttatta caacttatag gttgaagctg taaccaattt atagtcatat
    13501 tatattttta tcatccagtt tattgaattc ttctcgttta cctcaattca ttttcaaggg
    13561 gaatca
//
LOCUS       DAYYFD010000077        13295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000077 DAYYFD010000000
VERSION     DAYYFD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.077"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(244..1116)
                     /gene="scrK"
                     /locus_tag="ACHA25_002166"
     CDS             complement(244..1116)
                     /gene="scrK"
                     /locus_tag="ACHA25_002166"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8763448.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(1380..3326)
                     /locus_tag="ACHA25_002167"
     CDS             complement(1380..3326)
                     /locus_tag="ACHA25_002167"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8763449.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            3511..4950
                     /locus_tag="ACHA25_002168"
     CDS             3511..4950
                     /locus_tag="ACHA25_002168"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8763450.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            4952..5914
                     /locus_tag="ACHA25_002169"
     CDS             4952..5914
                     /locus_tag="ACHA25_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8763451.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(6084..7510)
                     /locus_tag="ACHA25_002170"
                     /pseudo
     CDS             complement(6084..7510)
                     /locus_tag="ACHA25_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7753..8208
                     /gene="tnpA"
                     /locus_tag="ACHA25_002171"
     CDS             7753..8208
                     /gene="tnpA"
                     /locus_tag="ACHA25_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8763452.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            8473..8571
                     /locus_tag="ACHA25_002172"
                     /pseudo
     CDS             8473..8571
                     /locus_tag="ACHA25_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8794..9024)
                     /locus_tag="ACHA25_002173"
     CDS             complement(8794..9024)
                     /locus_tag="ACHA25_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8763453.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(9254..10072)
                     /locus_tag="ACHA25_002174"
     CDS             complement(9254..10072)
                     /locus_tag="ACHA25_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8763454.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            10233..10922
                     /locus_tag="ACHA25_002175"
     CDS             10233..10922
                     /locus_tag="ACHA25_002175"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8763455.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            10936..12438
                     /locus_tag="ACHA25_002176"
     CDS             10936..12438
                     /locus_tag="ACHA25_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8763456.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            12451..12927
                     /locus_tag="ACHA25_002177"
     CDS             12451..12927
                     /locus_tag="ACHA25_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8763457.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(13024..13134)
                     /locus_tag="ACHA25_002178"
                     /pseudo
     CDS             complement(13024..13134)
                     /locus_tag="ACHA25_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4361 a         2220 c         2350 g         4364 t
ORIGIN      
        1 atttgttaaa aaggtccaaa aaaatatccg cacccccttt tttttgtaaa aaactattgc
       61 acaatacaag tggcctacgg ctatcgaaat ttccataact ttcgagcaag aatttatctc
      121 attcaaggct tgatcttcca agtaaaacaa aaaccagtta agcactctgc ctaactggtt
      181 atgactaaat tttcagttgt atctttctct accaacacta aatgtcgaag agcctttttt
      241 cgtttatgaa tgtaagaccg cttctcgagc caatgccaaa caaccgagcg ttccagcgtt
      301 gtcttctaat gctggggtca cgatatattt gcttaactca ggtgtttcga catacccatt
      361 gaccaatttt gcaaatgcgt cgtggacgtt ttgcagcata tggcgttgtt tcatcacacc
      421 accgccaaaa atgatcacat ctggagaaaa catcaaggtt gtattgtagg cacattgagc
      481 aatataggag gcttcgattt cccaataagg atcatcttct gcaacctctt gtcctttttt
      541 ccctaagcgg ttttcgatgg cgggtccagc ggccatccct tctaaacagt tgccatgaaa
      601 tggacagctg ccagcaaagt catcttttgg atgagggacg acgagcatat gccccatttc
      661 tggatgacta aacccttcga taaagcgccc ttcttgtaag gcaccgccgc caacacccgt
      721 accgatcgta tagtaaacga cactgctcaa tcctttgcca ctgccgaaga catactcacc
      781 ataggcagcc gcattgacat ctgttgtcca agaaatcggg atcggaaatt cttttttcat
      841 ggtaccgatg aaatcaaaat tttgccatgc caatttcggg gtcgaggtaa tgtacccata
      901 agtggcggaa tcgcgatgga tatcgatagg cccaaatgag ccaatcccaa tccctgctaa
      961 atctgcttgg tattgtttga agaagtcaat gactaaaggc attgtttcct caggtgtcgt
     1021 tgtcggaaag ctcactcttt ccttgatcgt catttcttca tcgccaatgg cacacacaaa
     1081 ttttgtgccg cctgcttcaa ttgcaccgta tagcatagac atttctccct ttattttaga
     1141 atgaaatcac taaataaatg actatatatc atatttaaat tattttttta tttagtaaca
     1201 tgacaagtaa ataactacta ttttttcttt ttgaattttt atcatttttt aaaattctaa
     1261 taatttttca tacaattgat atatgaaaag attaaataaa atgttattct caaatatttg
     1321 attaatctat attatttgat tttggaaaga aaattccatc tttactcagg gaaatatatt
     1381 tatttgattt caagtaaatc agtaccctca gtgactgtat tttcagctac tatatttact
     1441 tctgaataat ccgaagtatt ggtcacaata atcattgttg tatcatcaag accttctttc
     1501 tcaatcattg aacggtcaaa tgctccaaga atatcgcctt ttctaacttt ttgattagta
     1561 gtaacttttt gtgtaaatcc ttttcctccc attgatacag tatcaatacc aatatgaatt
     1621 agaacttcag caccattatt tgatttaata ccataagcat gtccagtttc aaacgtgagt
     1681 tgaatagtac catctactgg agaatggacc gtgtttccat ttggtttaat ggcaatccct
     1741 tttcccatag cttctgatga aaagactgga tcatttactg aagaaagact aatcacttta
     1801 ccagaaatgg gactaacaat tgtttctgat gaaacttttg tttgtgttga ttcaacatca
     1861 gtagatattg gatctgttgt tgctactttt gaagaattaa ctggtgtttg ttcagcaatt
     1921 tcttctgctt taaagatacc agcagctttt gttttaccgt agaagtaagt aattgcaaaa
     1981 gcaactacaa agctaatcaa ttcacacaat acgtaaaatg gaatagatgt tgcattaatt
     2041 gaaagaaatc ccaagaaacc tgctgaacca agtgaaacag caactacttt aagtaatcct
     2101 gctactgctg tagcaattcc tgaaccaatt aaagcacaaa agaatggaaa gcggtatttt
     2161 aagttaactc cgaacaaggc aggctctgta attccaagaa gtgctgagac acccgcagag
     2221 gaagcaagac tttttgtctt agaattttta gttaagaacc aaatggcaaa tgttgcagca
     2281 ccttgagcaa cgtttgccat agatgcaact acaaagataa agtctccatg tccgatacca
     2341 tttgtccacg ctgaaagtaa ttgagtttca atagctggaa atgattgatg taaaccagtc
     2401 atgactataa gtgaatatgt tcctccaaaa atacccattc caataaatcc agtagtgtta
     2461 tatagccaaa caataatgtc tgtaattcca ttagacaaca ataacattac agggccaata
     2521 actgtgaaag tcaaaaatcc agtaatcatt actgataata atggcgtgaa tgtaaagtca
     2581 atagatgatg gcaatttttt atgaaagaat ttttctaata tagataatat ccaaacagct
     2641 gctaaaactg gaataacttg ataagcatag tttgtttgtg aaacatgata accaaaaata
     2701 ttccaatatt cagttgagcc accaagatct ggtgttgtca tgatcatacc aattgcagca
     2761 ccaagaaatt ggtttgctcc aaaacgttta gcagctgaaa tacctactaa aattggtaag
     2821 aacatgaatg gtgcagcgga catcagttgg atcattgagg aaataccttc aatcgcagga
     2881 tacatttctt caattgattt tggtccaaaa agtccagcag ctgttaaaaa gttttgtaat
     2941 gccattaata aaccacctgc aacaagggca ggtataattg gaacaaaaat atctgataat
     3001 aatttaatta atgccataat ggggttgaat tttttatctt tactagcaat ttgttttaaa
     3061 tcatctgttg aaatttccga aagtccagtt tttttgataa gttcgtcata aacgaaatta
     3121 acatcacctg caccgataat cacttgatat tgtccatcaa ttttaaatgt gcctttcaca
     3181 tctggatttt cgtctaaagc tttttgattc actttcgtgt catcttttag tacgagacga
     3241 agacgtgtag cgcagtgtgc agctgcaata aggttatctt ttccgaccgc ttgtattacg
     3301 tcggaagcta ctttactgta attcattcga atcactcctt ttttattttt acgtgaaaac
     3361 ggttcactga aatgcaaatt tattttagca tatcttaaaa atatgtcaaa cgtttgtcat
     3421 atagtttatt tttttaaatg aaatgattga tttttcaacg gaaaacgttt accatactag
     3481 ttgtaaaaag ataaattgaa ggagtaaaat atgaacttac ctcgagaaat acgatatcgt
     3541 gcttattctg attggactaa agatgagatg gataaaatta aggataatgt aaaacgatca
     3601 ccttggcgtg ctagctatca tatcgaacct aaaacaggat tgttaaacga tcctaatggt
     3661 ttttcttttt ttaacggaaa atatacgctt ttttatcaaa attggccatt tggagctgct
     3721 catgggttga aagaatgggt acacaatgaa tcagatgatt tagttcattt tcatgtaaca
     3781 ggagcagaac ttcttccaga cacaaaacga gatagccacg gcgcttattc cggttctgca
     3841 tatgaaatag aggggaatct attcttactc tatacaggaa acgttcgaaa tgaaaattgg
     3901 gaacgtgata cacttcaaat cggagcatgg atggataaag actacaacat tacgaaaggt
     3961 gagaatgttc ttattcataa acctgctgat gtaactgatc attttcgtga tccacaaatg
     4021 tttaattatg aaggacaatt gtatgcgatt gttggagcac aaaatttaga aaaatcaggc
     4081 tttatcaaac tttacaaagc ggatgacaat aacgtggaaa catgggagga agttggcaac
     4141 cttgattttg gcggcactgg ttcagaatac atgattgagt gtccaaatct agtatttgta
     4201 gacaaaaaac ctgttctcat ttattgtcct caagggttgg ataaatctga gctaaattat
     4261 gggaatatct atccgaatac ttttaagatc tgtcaatcgc ttgacataga aaaagtaaaa
     4321 cttgtgggcg cttcagaaat tcaaaatctt gattatggat ttgaagcgta tgcgactcaa
     4381 ggattcaata ctcctgatgg tcgtgctctg attgtcagtt ggattggact acctgatatc
     4441 gattatccaa ctgataagta tgattatcag ggagcaatga gtcttgtaaa agaactttca
     4501 ataaaagatg gtaaacttta ccaatatcca gtagaagcta ttacatcatt acgtgctagt
     4561 tctgaacaat ttacagcaaa agcagaaaca aataatactt atgagcttga actaactttt
     4621 cctccaaatc agaaatcgga gttacttctt ttctctgata gtaaaggaaa tggacttagc
     4681 ttgattgtag ataccaaaga aggaaaaatt attttagatc gcagcaaagc aggcattcaa
     4741 tatgccactg actttggtac gattcgtgaa tgctcaattg atactaaaga aacaactgct
     4801 aacatttttg tagataactc aatcattgag atttttatta ataaaggaga aaaagtgttt
     4861 actagtcgtg tattccctga aaatcaccaa aatggtattc aaatcaaatc aggcgaacct
     4921 actggaaaat actttgaatt aaaatattaa aatggtagca aaattaactg atgtcgctga
     4981 attagctgga gttagcccaa caaccgtttc acgcgtcatt aataataagg gatatttatc
     5041 agaaaaaaca aaacaaaaag ttcatgaagc tatgaaaact cttggttata aaccaaataa
     5101 tttagcacgt ggacttcaag gaaagtcacc tcagcttatt ggtcttattt ttcctaatat
     5161 tagtaatatc ttttactctg aactcattga gtatctcgaa atagagttat ttaaacatgg
     5221 atataaaacg attatttgca atagtcaaaa tgatcctgct aaagagagag aatacattga
     5281 aatgcttgaa gccaaccaag ttgatggcat aatctcatca agccataatc ttggaattgc
     5341 tgattatgag cgtgtgagag cacctattgt tgcttttgat agaaatttag ctcctaatgt
     5401 ttccattgtt tcttcagata actttgaagg gggaaaactt gcagctaaga ctttacaaaa
     5461 aaatggttgt caaaatacca ttatgattac aggaaatgat aacacggatt ctcccacaga
     5521 attacgtgca ctaggttttt cttttcaaaa tccaaatgga aaaattttca aaattcctaa
     5581 taatctgtct actatccgtc gtgaaatgga aataaagtca actattataa atcacaaacc
     5641 agatggtatt tttgtttctg atgatttaac agccattctc actatgaaaa ttgctcatca
     5701 acttaaactt aatatcccag aagatttaaa aattattgga tacgatggta cctcttttat
     5761 tgagaaattt ttcccacaat tgacaacaat aagacaacca attgatgaaa ttgcaagtct
     5821 tattgttgat attcttctga aaaaaattaa aggcgaaaag acaaataagg attatatttt
     5881 accaatctca atactttctg gaggaagtat ttaacgttaa attaattttt taatgtctat
     5941 aaaattcgtt agaaaataca atttgtagat atccaattga tttgtaaaca aaaaggagtt
     6001 gtcaacttgt agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactccttt
     6061 tttcatgctc taaaatgata tttttacaca actgttacgg ttgttttacg aaattggacg
     6121 ggactttgcc agtttaattt ctgttttatc cgttcattat tgtagtaata gatatagcta
     6181 tcgatggctg attttaattc ctcgaaacag ttataaactt tcccgtgaaa aatctcttgt
     6241 tttaataagc caaaaaaatt ctccataagt gaattatcta agcagttccc tttccgagac
     6301 atgctttgaa agattttgta ctccttcaat ttgtttctat aagctttcat ttgatacgcc
     6361 caaccttgat ctgagtgaaa cgtacgacga tagggacaat cgttagtggt ctggatagct
     6421 tcctccaaac cttccattac cgctaatgca ttgggctttt cagagatacg ataggataaa
     6481 atctcactat tgaacatatc cataaaagga tctagataca atttcttttg acggataatg
     6541 cctttggcat ccacttcatg gtatttaaat tcagttgtat ccgtcgtgat tttctgatgg
     6601 cagatactgg tgtagaaacg acgatggatc cgatttttgg ctactgctcc gacttgtcct
     6661 cgataagaat tgtagcgccg tgatttacgt gtgtatgtcg ttacttttat tcctagcttc
     6721 ttgatcaggc gctgaacttt ctttttattg atagagaaac cgcggtttct tagttcattg
     6781 gtcatccgta aacagccata atccttgtgc ttctcccgaa ctttaagcat ttccgcttca
     6841 atctcttgat ctggattttt acggtccaat cgtttttgcc agtacataaa gattgcctta
     6901 gggaacttta aagtttctag aagctcaact aatttaaacg gtcctcggag gcttgcgatg
     6961 attcgtgcga ttgcttcatt cgtcgttgtt gggcttcctg ttttcgcagc ttcctcaatt
     7021 cttttaaaaa ggcattttca atttgaaggg aacggactcg tttctccaac tcctcgatgc
     7081 gttcacgttc tttagacgtt gctttagttg tagatttttt cttatttttg ggttcatttt
     7141 tcttagacaa gataggtgga catcctttcg tttttgagag tccatcgatc ccaccctcac
     7201 ggaattgacg catccaacta gcgatcaaat tagggttgtt catgttaagt gtgttggcaa
     7261 cttcacgata ggacagctca cttgttagat ataactctat cgcatccagc ttaaattgaa
     7321 cagaatattt tttcttttgt cgctttctta ctagaccctc ttcaccaaat tcttgatagg
     7381 cattgatcca gcgcttcact tgtgatggag ccttaatgga atatttcttt gccagataat
     7441 cacttccgcc tttaccatcc aagtaatcat gaacgatctt taatttaagt tcaaaagtat
     7501 acttagccat aaaaaatacc gacctccaaa agttagattt tttggtctaa cttttggggg
     7561 tcggtacaaa acttaaaagt tttagaccct tttattttct taatgacttc agtcagtttt
     7621 gtgcgtaaag cgtgcgaaac aagaaaaccg cgcgtttcta cgcgtggaag aagtatcttt
     7681 agcgataaaa actcctttcg atatactagt gagtggctac caaccgctca tcagatcgaa
     7741 aggagttttt tcatgtcaaa tgacgataaa agtttagcat atactcgttg gaattgtaag
     7801 tatcatatta tttttacacc aaaatatcaa cgaaaagtaa tctatgggca attacgaaaa
     7861 gatcttggaa aaatacttag gaagttgtgc gaaatgaaag atgtggaaat catagaagca
     7921 catgcaatgc tagatcatat tcacatgctt gttcgagtac caccaaaaat atgtatatcc
     7981 agttttatgg ggtatctgaa aggtcgtagt gcggtactaa tccacgagca gcacgcaaat
     8041 ctcaaatata aatacggaaa caaatccttt tggtcgaaga gatactatgt gagtacagtc
     8101 ggattgaatc aaaagacaaa tgaaaaatac atccgtgaac aagaagctga tgatcgcatt
     8161 cgatatatta taagtaaaag agagtacgta gaaccattta aaaaataaag agaagtaagt
     8221 gagtgaagat gagcagttgg cggtctttta aaaatcccct tttaaggggc agcgagtgtc
     8281 gaccccttct agggggatta aaaatacacc cgtcctcacg ggtgtatggg ttactgatag
     8341 acttgatttt aactggtgtc agaaacttat gtccagaaag ggtgatgaaa ttttggcaat
     8401 ttacggctat tcaagaatta gcacaagcaa ccaagattac aaaacacaaa ttcaaacatt
     8461 agaaaatgct ggcgcagaaa taatcttcct agaaaatcac tggattatcg aacacctttg
     8521 gaagtatttt tgagttacgt aagtattgat gatctgtcta acttaatttg acaattaaga
     8581 gtatatttac ttcagatggt aaggcaacaa aataaaatac aataggcaga tgaactaata
     8641 aataaaatct gtattaaata cagataaatt aatataaaaa tcaaaaggat cataaacttt
     8701 taacaagtga tgaaatgggt ggaaattgat tcttatagac agtttgtctc ataacattga
     8761 agtattttgc aattttagca ttgaaacact tttttaatct tcaacgttgt tcttataaac
     8821 aagatgattg ggaatctttt gaattaacac tgtatagctt agaacaccct ttatagaaaa
     8881 cattcgctta gcaatcttaa taattccttg tgcccgatgt cgtttagatt cagtaggttc
     8941 ctgctcatcg atcatggaaa aaatttgaag gatcagatcc ttaataaatt tatttaagag
     9001 aggatgatcc attgcttcat ccatactcga taaactagtg ataatgagct acacgttttt
     9061 ctttcaagtt gatcaatagc ttccacgata aaaatatctt tagataaaat caagaaccgt
     9121 aggaactaca gggatatttg gtcaataaga gccaccgcta ttagtaaaaa ataagctatc
     9181 aagtatgctc tcatcccaag aattgcctac ttcaatcgtt aaaaaccgtt aggtttagct
     9241 aagtgggggt agttcactca atacttttat caataataga gcgcaaaact ttttctctta
     9301 attcaactga atcaatatta ttgttttgtt cataaccatg aactaataga tcacaaatat
     9361 aaagttgtga tatttttcca gctaaagatc caccattaag aaattcttca atggctgttt
     9421 gtaaaactaa gtctgcagac tttcctatag gtgaatgaat atagttagtt atagcaataa
     9481 tttttgcgcc atttttttta gctattttca atgaatcata agtatctttc gttttcccag
     9541 ataaagaaaa aatgattact aaatcattaa ctgttaataa agaagcgact tgggcttgaa
     9601 aatgagggtc tagtacagct tttgcttgaa ctcctacgcg aagaaacata ttttctaaat
     9661 caagactagt atttccacta gatcccactc caaatatata tatgtttttt gattgagtga
     9721 tcattttaat ggcttcattc aatttttctt cgtctaaaag cctaatagtg gaatagagag
     9781 cttctgttaa actattagca atttcatcat agtactttac actagtcgga tgttcttttt
     9841 tttgactaaa gtcttccttt gcaatttcaa tttttaaatc agaaaaacca gaaaaaccaa
     9901 ttttttgaca aaagcgaata atagtagcat cgccaacttt tgtgtttttc ttaatatcac
     9961 tcatcgtact ataaataata gttttcccag aatttaaaat aaaatcagcg actttttttt
    10021 cagacttcgt taaaaatgga taattttctt taattaaata gatataactc atacaaactc
    10081 ctctacaatt tcaacttttc tccattatac tccaaaataa taaaaaaaac tcctaaatac
    10141 tcccttttta tctttacttt tatgaaagcg gatgctatat tgtttttgga gttgttatcc
    10201 aaaaataata aaaaattgga gtgtttagga taatggataa aaagaggttt ttagatctta
    10261 tttcaaaagg tttgattgtt tcctgtcaag cattacctgg tgaaccattt tacaaagagg
    10321 aagaaagttt aatgccttat tttgctttgg ctgctcaaag ggctggagca gttggtatac
    10381 gtgcaaattc tgttcgagat attattgaaa ttagaaagtg tgtatcatta ccaatgattg
    10441 gaattattaa aagagattat ccaccagaat ctccattcat tactgccact atgagagaag
    10501 tagatgaatt agtagaaacc ggtgttgagg taatcgctct tgattgcact cttagaaatc
    10561 gacatgatgg actatctata aattctttta ttactaaaat aaaagaaaaa tatcctcatc
    10621 agttatttat ggcagatatt tctacatttg aagaaggcaa aaatgcatat aaagcaggaa
    10681 tagattttgt aggaactact ttaagtggat atactgagga aagtaaaaaa caagatgggc
    10741 cagatataga tttgattaaa aaattggtag aagaagggat ttgtgtaatt gctgagggaa
    10801 ggattcatac tccagaacaa gcaaaagaga taaaaaaaat aggagttgca ggtattgttg
    10861 tgggtggtgc tattactcga ccacaggaaa ttgcatcaag atttgttaaa gcattaagtt
    10921 agggggagaa ataaaatgtt taaaaaattt tcacaattag gacgcgcttt catgttacca
    10981 attgctatat taccagtagc cggattacta ttgggactgg gaggagcgtt aacaaatgag
    11041 agtgctataa atgcttatcc atttttagat caaccgtggt tgcatacaat tttaagtatt
    11101 atgagttatg ctggaaatgc agtttttgca aacctagcac taatttttgc aattggtatt
    11161 gctgtaggac ttgcaaatgg agataaagga acagctggtt tagctggtgg agtagcttat
    11221 ctagtatata ctgcaactat tagtgggttt cttgaacttt tttcagcaaa agatgcaaca
    11281 ctagatacag gtgtagtggg atcaattgtt gttggtggta ctgttgcatt tttacacaat
    11341 cgttatagaa aaattgaatt accacaattc ttaggttttt ttggaggatc aagatttatt
    11401 ccgattgttt ctagtttagc tgcaatcatt attggttctt tcttttattt aatttggcca
    11461 ccaattcaaa atggtttagt agttgctggt gaacaaattg cccaaatggg tagtttagga
    11521 acttttttat atggtttttt attgcgttta acaggtgctg ttggactaca ccacaccatt
    11581 taccctttgt tctggtatac atctttaggt ggtaccgaaa ctgtagctgg atcaacaatt
    11641 gctggagccc aaaatatttt ctttgcccaa ctagctgatc caaaccacac tggtctgttt
    11701 acttatggta ctagattttt tgcaggaaga tttgcaacaa tgatgtttgg attaccagca
    11761 gcatgttatg caatgtatcg tgcaattcct aagaaaaatc gtaaaaagaa tggtgggctt
    11821 tattttagcg gggcccttac atcattttta actggaatta ctgaaccagt tgaatatatg
    11881 tttttatttg ttgctccttg gctttatgtg attcatgcat tcttagatgg attatctttt
    11941 tattttgctg atatcttaaa tattcgtgta gggaattcct tttcaggtgg tttgatcgac
    12001 tttttattat tcggtgtact tcaagggaat gacaaaacta attggttaaa attaattcct
    12061 tttggtattg catgggcatt tatatatttc tttgttttta cattctttat caaaaaattt
    12121 aaggtagcta ttcctggaat ggaagaagaa tcagaagttc attctgaaaa agtaataaac
    12181 aaaaatacat cattacatga agagtctctt atcattattg aagctcttgg tggaaaagaa
    12241 aatattgagt ctgttgaagc ttgtgcaact cgtttgcgtg ttgctgttac gaatggggag
    12301 attattcaaa aacaaacaat aaaagacact ggtgcaactg ctgtcttcga agtgaagggt
    12361 ggcatccaag ctgtttttgg agggaaagct gacttattaa gtcaagaaat caatcaaata
    12421 ttaggaaaag ataattagga ggtttttaat atgtttgaaa ttttcaaaaa gaaaaaacaa
    12481 gaaacttttt atgcaccttg ttccggaaaa atcatttcca tcacagaagt aaatgatcct
    12541 gtcttttcac aaaaattact aggtgatggt tttgccgtca tacctgatgg ttcaaaaatt
    12601 tatgctccat tggctggtcg agtaacaaat atatttccta ctaaacatgc tattaatata
    12661 aaaagcaact caggcattga ttatttgatt catattggaa ttgatacagt tgaattacaa
    12721 ggagagcctt tttcgatttg tgttcaagaa aatgatgagg ttactcctga aacctcgttg
    12781 gttgatgtgg attttgacaa aataataaaa tctggtaaag atacttctgt tatagttgtt
    12841 tttccggaga aaaatcagtt caatgattta ataatagata atggtcgaat tgctcatggg
    12901 aattcatgtg gaaaaatcac tttttaaaaa agtgtatata aaaagctata aaataagcta
    12961 aataggatat ttgtcaaatg aagttggtga acagaaagaa ctgttttctt tctgttcatt
    13021 tttttatttt attagtaatg attccccatg ccaaatcatg atccgtaatt taaagttata
    13081 aaagcttcga aatccattgg catttcgttg gaataccttg atatgattat tcaaatataa
    13141 aatatagaga gaagagagag agggaagata tttataaagg gcaaagttat gtttactcaa
    13201 cttaggaaat agaaatagat caatttatat tctcttgtca atcgaatatg aaccttagca
    13261 ctatatacca aatatagtaa aatgctgaaa gatgt
//
LOCUS       DAYYFD010000078        12925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000078 DAYYFD010000000
VERSION     DAYYFD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.078"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1557)
                     /locus_tag="ACHA25_002179"
     CDS             complement(52..1557)
                     /locus_tag="ACHA25_002179"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8763458.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1649..2239)
                     /locus_tag="ACHA25_002180"
     CDS             complement(1649..2239)
                     /locus_tag="ACHA25_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8763459.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2418..4064
                     /locus_tag="ACHA25_002181"
     CDS             2418..4064
                     /locus_tag="ACHA25_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8763460.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4113..4832
                     /locus_tag="ACHA25_002182"
     CDS             4113..4832
                     /locus_tag="ACHA25_002182"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8763461.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5311..6258
                     /locus_tag="ACHA25_002183"
     CDS             5311..6258
                     /locus_tag="ACHA25_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8763462.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6270..7190
                     /locus_tag="ACHA25_002184"
     CDS             6270..7190
                     /locus_tag="ACHA25_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8763463.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7231..8688
                     /locus_tag="ACHA25_002185"
     CDS             7231..8688
                     /locus_tag="ACHA25_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8763464.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9033..9647
                     /locus_tag="ACHA25_002186"
     CDS             9033..9647
                     /locus_tag="ACHA25_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8763465.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9640..11364
                     /locus_tag="ACHA25_002187"
     CDS             9640..11364
                     /locus_tag="ACHA25_002187"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8763466.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11361..12812
                     /locus_tag="ACHA25_002188"
     CDS             11361..12812
                     /locus_tag="ACHA25_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8763467.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         4190 a         2237 c         2591 g         3907 t
ORIGIN      
        1 cacagtccct ttttttctta gaaatgggaa accactagta tataaatacc attattcttc
       61 aatcaaccgc ttcacgattg cataatcccc ttcataaggt tcatctacac cattgatttt
      121 ttgataggga tccacgcctt ttcccgcaat aacgactgaa tcttgcggag cgctttctgc
      181 taaagcgcgt cgaatcgctt caggacgatc catttcataa tgaacgatca aatctggatt
      241 agtaattgct tcattgattt cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc
      301 tgccgtcaaa aaggcgatgt cagctgtttc tgatagcact tttccaaaat catgtcgacg
      361 ggagattgct ttgtttcctg ggctgcctaa tacaacaatt acacggccat ctggatgttc
      421 atttttggca aattccagca atgttttcaa gcttagataa ttatgtgcat agtccacata
      481 gacatgcgct ccattggatt ggatcaattg atccattcgg cctggtacac gagcttcttt
      541 taatccgtct tgtgcatctt gactggaggc acctgttagt gctgcggcaa tgatcgcact
      601 ggctgcattt tcttggttga atcctccagc cagcaaaata tcataagcag tatcgcagat
      661 agctaagcga tcttcatgag aagataaagt aaaaccgtga gtacctttat ctgaacggat
      721 cacttgataa ttgctttctt ccgaacgacc ataagtgatc gttggaatct ggaatagatc
      781 ggctgtttcc ctaagtaaat caaaatagtc actttcatga tttaaaacaa ctgtttttga
      841 attcaagatc aattgtcgct tgcaatagaa atagtcatca aatgtcggat gttcgatcgg
      901 actgatatgg tcaggcgaga tattcaagaa gattccgaca tctaaagtca agccgtatac
      961 gcgttgtgtt ttgtaagctt gtgaagaaac ttccatcact agatgagtca taccattttc
     1021 aactgcttct gacatcatac gatataaatc taatgactca ggcgttgtca aatgggattt
     1081 gaaatatgtt ttgccatcta acgttgtatt catagtagaa agcaatgctg tttttttatt
     1141 tgttgtgtga tccaagattg cttttgtaaa gtacgcagca gttgttttac cttttgttcc
     1201 agtgaatccg atcactttca atttattttg cggatagtcg taaaaagcca tagcaagaag
     1261 cgccattgct ttacggatat ccgttacgat gattcctact gctgaatttt cgtatggttg
     1321 ttctgataca taatattgaa taccctgttc gattgctgaa tccaaatatt cttctttgaa
     1381 attcattcct ttacagaaaa acaaagtaga agcatcagct tttcttgaat cataacttaa
     1441 ctgaagaaat tcagcttttt ctggtacatc aagatgccag cctcttgttg aaacaaattc
     1501 ttttaataga ttttctttta atagtaaatg acggatgtct tctaaagaga agctcattga
     1561 ttgatccctc gctttattca atttcttggt ttattatagc acactctttt taatatatcg
     1621 tttgtttccg ctatgacaaa atccagcttt attcaaattt tcttgacaca ggggacaagc
     1681 ggatgagtat tttcataaac aatgaactga ctgtaataga taggagaacg aatagtgtct
     1741 ccaacaatcc aaaaacataa taattcaaaa agacgataag actattggta agtaaaagtc
     1801 cttgcggcat tggaagccga aaaaatttgt ggaaaataat cggcggtatc gttataccgc
     1861 catttgaggc cccgactgtg tacaatgtgt aaaccccaag actgaacaaa agacttcctg
     1921 aaatcagtga aaagaaatgt gaggagatca tttccacttt tggaacaatc tctaaaacaa
     1981 caggaaaaag cagacttccc gctacagtct taaaaaatac tgattttcct aataggacag
     2041 cggctagaag cagcatcata agattgatgc tccaaacagt aactgagcgt tggatagaaa
     2101 aggccgtttc aactaaatgt ccgatggcgc ccactccggc ggaagctata tcgtgaggga
     2161 gcaagaacca attcaatcca actgtgataa agaaaagacc taaaaatata acgggtagtt
     2221 tttttatttc ttgtgtcatt gatatctcct ataaatgtta gccataaaag gctgtatatt
     2281 tctttacttt accaaaaaaa cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt
     2341 atataaaatc gattgccagt ttctgtttta ccaattaaaa tgatagtaat gttttgtaga
     2401 aagttggaat ctgaccgatg gataatcaaa gaaaaccagc attagatcaa ttaacaccgc
     2461 aagaaaaaat ggcacgaggc tctgcttgga tgaccgccag taatatcatt tcaagattgc
     2521 taggcgctgt ctatatcatt ccctggtatg cttggatggg cgaaaatgct aaggctgcca
     2581 atggattatt caatatggga tacaatatat atgcattgtt tctactgatc tctacagcag
     2641 gaatacctgc tgcgattgca aaacaaactg cacgctataa ttcattgaat gaatatggca
     2701 cttcacgacg attatttatc cgtgcattac agatgatggg cgggctaggc ctgttgtttg
     2761 ctttgttcat gtacatcgcc tctccttggc tagctcatgc ttctggtggc ggagaagaac
     2821 tgattccaac gatgcgttct ttgagtatcg ctgtattggt ttttccttgt atgagtgtga
     2881 tacgagggta ttttcaaggc aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag
     2941 aacaggtggc acgtattttt tatatgctat tggctacatt catcatcatg aaagtcatgg
     3001 atggtgacta tgtgacggcg gtcacgcaat caactttcgc ggcatttatc ggtatgctag
     3061 caagtattct tgtccttttg tattttttga agaaacatca agcgtataca tcggcgttta
     3121 ttcactacag cgaaaacaaa ataaatattg caacgaagga attgttgtta gataccgtta
     3181 aagaagcgat tccgtttatt attgtgggtt caggggtaac gatcttcaaa ttagtcgatc
     3241 agttcacttt tatgaagatc atgtctgcat ccaccgagta ttccaatgca cagttactgg
     3301 atcttttttc gatttttagt gccaatccag ataagttgac gatggtagtt attgcattgg
     3361 caacatcgat cgcgtctact gggttaccgt tgattacaga agctgttacg ttgaaagatc
     3421 gagtaggtct ggcaaaattg acaagcggaa atttacagtt gttttcattt tttatgtttc
     3481 cggcgacatt tggtgttatg ttacttgcct atccgctaaa tacattattt tatacaccgg
     3541 attctctagg aagtaatgta ctgatccaag caagttttgt aggactcttc ctaggactat
     3601 atatgctagt ttccaacatg ctgcaaggaa tgtttgaaaa caaggcggca attaattatt
     3661 tggttattgg gtttctagtg aaactccttc ttcaataccc agccattcgt ttatttgaag
     3721 tatacggccc tttattggcg acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa
     3781 agagaatcca tgaagtagcc cgctttaaac caagttttgt ctggcgcagg acattgctga
     3841 tctttattct taccttgatt atgcttttgg cttcgtggat cacaaagatg atttttggtg
     3901 cttttttaaa cgaagatagc aaattccagt ctctattgtt gattcttgta gtagcaggta
     3961 taggggcttt agtgtatggt tatttagctt tgaaaatccg cttggcagaa agactactag
     4021 gaccaggaat agcgcgttta cgacataaat taaaaataaa ataagtgaat cagaagagct
     4081 agattgatgc tcttttgaaa tgggggaaac gcatgcgttt agataagttt ttagcagata
     4141 tgggacttgg cagccgcaaa gaagtcaaag gattgatcaa aaaaggaacg atcgaagtca
     4201 atggacaagt ggtgaaaagc gataaagcgc aagtaaatga attcgaagat gcggttactt
     4261 atttaggaga aatcattacg tatcaaaaag atttttacta cattttgcat aaacctgcag
     4321 gagtcatttc tgctactcag gataatcatg atcaaaccgt aatcgattta ttggaagacc
     4381 aggattatcg ggaagatctt tttccagtcg gccgattaga taaagacaca gagggcctct
     4441 tgattttaac gaatgacggg gtgtttgccc atcagttact atcacctaaa aaacatgtgg
     4501 agaaagaata tctagctgag gttagcggta taatgacaga agaagatatc cgtttatttg
     4561 cagaaggact gattattgat ggcgatgagc agacattgcc ggcaaaactc caaattgatc
     4621 aagtagatca agagacagaa acttcaatta tccgtctgat tttgcatgaa ggaaagtttc
     4681 accaagttaa gcgaatggtc aaagcagtag gaaaagaagt cttatattta aaaagaatcc
     4741 gtatgggaaa attttggctg cctaaagatt tgaataaagg gcaatatcga cccatgacca
     4801 aagaagagct agaacaagtg agagaaaaat aattgaatgg atagctaaaa gaataagcag
     4861 agtagttgta gcgccatctg aaaagcagac ttgtccgtct gactttcgga tggctttttt
     4921 gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttgt tcggaataag
     4981 ggactgtgac aagacttttg ttacagtccc cattgttcct tgtgactcag agttagatag
     5041 taaggacacg atctccctaa tactcaggcc aaaacgctga attcacaacc tctgataaac
     5101 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     5161 tttcccgttt ttttctctaa tactcaggcc aaaacgctga tttcacaacc tctcctatac
     5221 tttgtttcat tttccctata ctttgtttat ttattctcat tattgaaagc gctataatta
     5281 cgttgcgaat gaatcgaaag gggtttttag atgaaaaaac gaaaggggtt caatcatttt
     5341 tttatgaatc tatggcgtta taaagcactt gtcctgatgg ctgtaccggg tatgctttgg
     5401 atgatttttt tcttttacat acctgtaatg gcgaatgtcg tagcattcaa aaattttcat
     5461 atttcagcag atgggttctt ggctagcttg agggaaagtc cttgggtggg ttgggaaaat
     5521 ttcaaatttt tattttcttc ggatcaagca tttctcatca ctaaaaatac aattttatat
     5581 aatgtagcat ttatcctttt gaatctgctg atctcagtcg tatttgcaat cattatgagc
     5641 gagttgagaa ataaacgact cgttaaagtt tatcaaacga tgtccttact accttatttt
     5701 ctatcttggg tcattatcag ttactttgtt tacgcttttt taagtcctga caaaggaata
     5761 ttcaatcagt ggatcactgg acatggagga gaagctgtca attggtataa tgaaccgaaa
     5821 tattggccgt ttatcttggt gttcattggt acatggaaag ggattggtta taacagcatt
     5881 atttactttg catctgttat gggaatcgat ccgacttatt atgaagctgc tatggttgat
     5941 ggagctagca aatggcagca gatcaaacat gtgacgatac cacaacttat tccactaatg
     6001 acgattttaa cgattttagc cgtaggaaac attttccgag ctgattttgg cctgttttat
     6061 aacattccta ggaattccgg tgcattatat gatgtgactc aagttttgga tacgtatatc
     6121 tacaatggcc tgacatccac aggagatttt ggtatgacgg ctgctgcagg actttaccaa
     6181 tcagttgtcg gctttgtctt gttgatgatc acaaatacga tcgcccgacg ttttgatagc
     6241 gattctgcat tattctaagg aggaaaaaaa tgaaaaaaaa gaaagtggag agagtccaag
     6301 tccgctcttt caataagacg gctaattttt tctctaatat aatgattgca ctctttgcta
     6361 tttcctgtat tttgccattt atatttgtta tcgccatctc ttttaccgat gagtccatat
     6421 tgtcgactca cgggtacagt ttttggccag aaaagttcag tatatttggt tatacgtact
     6481 tgttcgacca gatgcaggat aaaattttcc aagcgctttt cgtcactgta cttgttactg
     6541 ttttaggtac actgatcaat agcacggcta cttctttata tgcttatgcg atttcacggt
     6601 caaactttcc attccgtcgt ttttttaccg ttttttgttt aattacgatg ttgttctcac
     6661 cagggatggt ggccaattat cttgttatga caaatcttct tcagttaaaa gatacgattt
     6721 gggcattgat cttgccaatg gctgtcagtc ccttcaatat tattgtgatg cgcacattct
     6781 ttaaacgttc ggtctctgat tcgattattg aatcggcacg gatcgatgga gctagcgaac
     6841 tgcgaatatt tgttcagatc gttttaccgt tagctgttcc aggtattgcg acaatcagtt
     6901 tatttgccgc attgggttac tggaatgact ggttcaatgc tttactatac atccaagatg
     6961 ataagctagt tccactacag tatcttttga tgaaaatcca aagtaatatt caatatctga
     7021 cacaaaattc aggagcagga agccaattga caggcggatt agcctcagtt ccaggagaat
     7081 ctgccagaat ggcgatcgta gtcatctcga cgttgcctat tgcgatcagc tatccatttt
     7141 tccagaagca ttttgtcaaa ggtctaacaa tcggcggagt aaaagaataa agaaacaaaa
     7201 atataaatca taaaattagg aggaagaaaa atgaagttat ggaagaaact agcctttaca
     7261 ggtgtttcag cgttgttgtt gggaacatta gcagcatgcg gaggttctgg ttctaaagac
     7321 caggcagaag ctagcaattc ggacactttg cagatgtatc aaatcggaga taaaccagat
     7381 aatttcgatc aattgatgga agtagcaaac aaacgaatcg aaaaagagat cggtgtaaaa
     7441 gtcaatatca attacattgg ctggggcgat tacgaaaaga aaatgaacgt catcatttct
     7501 tccggggaaa attatgatat cgcctttgcg aacaactatg tttccaatgc ccaaaaagga
     7561 gcctttgctg atttgactga attagcgcct aagtacgctg aaaaagctta taatgattta
     7621 gatgaagcat atattaaagg aaatctggtc aatggaaaat tatatgcttt cccagtcaat
     7681 ggaaatgtct ttgctcaaca agtacttact tttaacaaag ctttactaga taaatacgat
     7741 ctttcaattg atggaatcca atcttacgct gatgcagaaa aagtcttgaa ggaattccat
     7801 gaaaaagaac caaacacggc agcttttgcc attggacaag gcttcaaagt ccaagggaac
     7861 tttgattatc ctttagggaa tacgttacca tttgctattg atctggatgg agatgattcg
     7921 aaaatcatca accaatacga taatgaaacg tatatcggac tgttacgtac aatgcatagc
     7981 tggtacaaac aaggactcat tccaagtgac gctgcaacga gcaataagga ttatccacta
     8041 gaaggaaata cttggctaat gcgtggagaa acacaaggac cgtatgatta tggcgataca
     8101 attttgacaa atgcagcaaa acaagaactt gtctcaaaag caattactgt tccactaaaa
     8161 tctacagctc aagcacaaat ggcaaatttt gtcgtatcaa atacttcaaa aaataaagaa
     8221 aaatcagtag aacttctagg gctgcttaac agtgatccag aattattgaa tgggcttgtt
     8281 tgggggatcg agggagaagc atgggaaaaa gtcgatggtt cagatgataa aatcaaattg
     8341 ctagatggtt atcagccaaa tacacatatg tctgcttgga atacaggaaa caacaagatt
     8401 ctatatacac aagagtctat tacggatgac atgattgcca aacgtgatca atcgattgct
     8461 gatgcagaaa cttcaccgat tttaggattc agcttcaaca ctgacagtgt caaaacagaa
     8521 ttaagtaata tttctaatgt catgaatcaa tatttagatg gattgaatac agggactgtt
     8581 gatccagatg aaacattacc gaaattgaaa gacgctttgg acaaggctgg ctatgataag
     8641 gtattgaaag aaatgcaaaa acaatatgat gagttccgac aagaataata atgaataaag
     8701 acaaaggggt tgtgacaaaa gacgtgtcac aacctcttgt tccgaataaa cggtgttcaa
     8761 aagccagttt ctcggtatta agctaaatat atgtaaaaat gtggaaagcc atattcacaa
     8821 aatttttctt actactcgaa actaagcggc tatttcacaa actcttggta aacggtgttc
     8881 aatcagctaa ccctcggtat tcgctcacaa aacgacaaaa tatgaggaac tatttttccg
     8941 tttgtttctc taataatcag gtcaaggtgc tgattgccca accttttttt gtataatgag
     9001 aaagtataaa cttaatgttt ggaggagaag atatgttagg gaaggttttg gaagagttat
     9061 ttatccggat ctgggtcatt ataaaactaa ccttgtattt ctggatatat acatttgccg
     9121 gcgggatcat ctttggatta ggtgcggcat ggaaaacagt caatgaacta ttttatttgt
     9181 acggattcga gtataaagaa atcacaataa aaagaggctg gaatatatat aaaagaaatt
     9241 ttttaagagg caatctgcta tttagtttat ttttatctgg tactgcttta ttgagttaca
     9301 atttatattt atctgttcaa atcaaagggc tcattttttt agctattgat tttattttac
     9361 tttttgcctt gctctgccta tttgctactt atcaatacag tatgatttta gatagtgaat
     9421 atacgatctc tgttcctaat ctgctaaagc taagttttat ttcggttttt tccagctttt
     9481 cgtcttttct aaaaataatt attggtagcg gtataatttt aggcgtgact tggcaattca
     9541 aaggattgat cctgtttggt gtcattggtc ttttgacggt ttggaatggt acgatgacaa
     9601 cacattggcg tgaagaatta gataaacagt tggaaagtta tgaataagtt tcggattcta
     9661 ttttcaaaag gaaccttact aaatcgtctg ttgcggaaat acgcggttat catgatcggt
     9721 gtaacgatga ctgctaccgt tatttttagt atccatactt gggaacaaaa tcaaaaacag
     9781 gcagaaaaca tgacaagtga tgccgttcaa agcacaagca gaatgctgaa tgataaaaca
     9841 accttgagta ggatcatcaa aaaccagctt gtagggaatt ccgaaaagat cgaaaatgtg
     9901 acgacctact taacaaaacc catagatcag tatctgatgt atgtatacga acagcaaaac
     9961 agtaccgatg aattagtttc tttcccaaat caaatcaaag atctctacgc aaattatgaa
    10021 gaactaagtg cgatatatat cgtgttgaat caactaccgg agtattatga atcttctaga
    10081 gaaaacaaag gtggggaaat aaaaaagggg acaccaaaat tactagacgc cttttatatc
    10141 aagttgccta tcacacaagg aggagctgag tcaggaagta tttttattgg ttttcaaaag
    10201 aaagaactag atatgatttt agaaaatcta acgtcattca atggactctc tttatacatg
    10261 atttctggaa cgacaaatcg actatacaca ttccatgata ataaaatagc taaagaaacg
    10321 tttgaaagac aagaaatgat catcacagag agtctgaaga aaaattcaca attgccgatt
    10381 gacgatctgg aaaaaaacaa ttttgttcaa caccaagaac tgtctgatga ttatcgtata
    10441 ttagctgtac tagataaaaa aagtgtaaaa atcgaaacgg gaaaaaatct tgccccgctg
    10501 atcattggta tcattatttt agacaatata ctgcttatct ttctttataa aacattcaaa
    10561 cgatattcac agcaagtatc tatcgtcatg caggccatgg atcaaacagc agcagggaat
    10621 ttagagacgc ggatcgacat aacaaacacg gaatatgaac tgaaggaact ttcaatcggt
    10681 atcaacgaaa tgttggatag catcaatcag tttgtagaag acatctacaa attagaaatc
    10741 aaacagcaag acgcacatat gcgggcgctt caagcacaga tcaatccaca ctttttatat
    10801 aatacattag aatatatccg aatgtatgca atcagcgaag gaagtgaaga attagcagat
    10861 gttgtgtatg cgttttccgc tttgcttcga aacaatacga atcaagagaa aacgatcact
    10921 ttaaaagaag aactggactt ttgcgaaaaa tatgtctatt tgtatcaaat gcgttatcct
    10981 aatcgagtag cctatcattt tatgatcgat cctgatttgg aaaagatcga agtacctaaa
    11041 tttgtgatcc agccattagt tgaaaattat tttaaacacg gtattgattt tactcgattc
    11101 gataatgcgc taagtgtcaa agtcttgcag gaaggcaaac gggtaaggat catcatcaaa
    11161 gataatggaa aagggatgac tgaaaaacgg ctaaaacaga ttgaagaaaa actgtctcat
    11221 cctaaagtgg aacttcacgg ttctatcggt ctgcaaaacg tgaatgaacg tttacgtgcc
    11281 agcttcggca gcagttatta tatgtcgctt gagaataatg agacaggtgg actgaccgtt
    11341 tctattacat tcaaggaggg atgagcgtgt atcaagtgtt attggtagat gatgaataca
    11401 tgattttgaa tgggttaaaa aagataattg attggcaaag tttaggcttt caaatagtag
    11461 caacagcaga gaatgccaaa gaaggactag ctgtacttga acagcgacag attgatttag
    11521 tagtaacgga tgtgacaatg ccagaaataa atggactgga atttattgaa gcggctcaaa
    11581 aagaacgtca taattttgaa tttatgattt tatcaggata ccaagaattc gattatttga
    11641 aaggaggtat gcaattaggc gccgtgaatt atttgatgaa acccgtgaat aagttcgaat
    11701 tggttgaaag cctcaagaaa atcaaaacac ggttagatca gcaaaatgaa caaaaaaatc
    11761 aacaagaaat ctatcaagaa ctgttattca gtcaatggtt gaatgaagaa ttggatgaaa
    11821 taagtgaaga agaaataata ggacaaataa gtgagaagca gcataggata gtattgattc
    11881 aactggcaag aactcatgat actcttatca atagatggtt gcaagaacgg caacaaatct
    11941 tttactatca acgaaattac ggagaccgtg tattgatcac gttgttgttg gaggcagatg
    12001 aagttgatgg cttctgttgt tttgttcaag aaaactttcc tgatcaggaa tggctgatca
    12061 gtatcgggga agagacagat tctttggaca aaatccctga aacattccaa caagcgaaag
    12121 acaatctaca gttgcatcaa ttttatggag ataaagacga gcatgttttt tacgcagtac
    12181 aagcagaaac aaaagatcaa accatcgatt tttcaagttt cagacgcgtg ctgcaaaata
    12241 aacaattgga agtcgctcaa aaaatgatca ctgatttttt tgaacagttt cagttagcag
    12301 ccatgatgcc ggaagatata cgatattcca gttttttact gtttatggag attcaacggg
    12361 aattgattga tctggaagat gaggaatatt tacaaggaat cgaaaaaatc catcaggcaa
    12421 aaacggtgca agaacttcag cagctacttc tgtcattcat caaaaagcat cagagacaga
    12481 aaaagtacag tgacaatgtg gaaaaagtaa tcgtaatcct gcaccagcat tatcaagaac
    12541 cacttacact caaagaagtg tcagaaaacc tgcatttaaa tgtcatgtat ctaggacagc
    12601 tgttcaaaaa agaaacgaaa aaaagttttt cagcttattt gaaccattta cgtatggaaa
    12661 aagcgaaaca actgcttttg cacagtaatc aaaacatcaa tgagatagca agtgagattg
    12721 gctacaacaa caccacgtat ttctctaaat tattcaagaa aatcgtcggc agatccccta
    12781 aagagtaccg agaaaacagt ggagaatcat aaaaaaaaaa gccaatgtaa tgaaataact
    12841 tataaaaaat tgtctagaaa tctaattttg ggctgttctt attacttgag gcaaggcagc
    12901 tttttcatga cctctcgata aacgg
//
LOCUS       DAYYFD010000079        12821 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000079 DAYYFD010000000
VERSION     DAYYFD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.079"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1083)
                     /locus_tag="ACHA25_002189"
     CDS             complement(<1..1083)
                     /locus_tag="ACHA25_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8763468.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQ"
     gene            complement(1205..1801)
                     /locus_tag="ACHA25_002190"
     CDS             complement(1205..1801)
                     /locus_tag="ACHA25_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8763469.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2001..4643)
                     /gene="alaS"
                     /locus_tag="ACHA25_002191"
     CDS             complement(2001..4643)
                     /gene="alaS"
                     /locus_tag="ACHA25_002191"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8763470.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5031..5462
                     /locus_tag="ACHA25_002192"
     CDS             5031..5462
                     /locus_tag="ACHA25_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8763471.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5507..6856)
                     /locus_tag="ACHA25_002193"
     CDS             complement(5507..6856)
                     /locus_tag="ACHA25_002193"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8763472.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6926..8089)
                     /locus_tag="ACHA25_002194"
     CDS             complement(6926..8089)
                     /locus_tag="ACHA25_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8763473.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8218..8541
                     /locus_tag="ACHA25_002195"
     CDS             8218..8541
                     /locus_tag="ACHA25_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763474.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8724..10073)
                     /locus_tag="ACHA25_002196"
     CDS             complement(8724..10073)
                     /locus_tag="ACHA25_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8763475.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10142..11095)
                     /locus_tag="ACHA25_002197"
     CDS             complement(10142..11095)
                     /locus_tag="ACHA25_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8763476.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11197..12387)
                     /locus_tag="ACHA25_002198"
     CDS             complement(11197..12387)
                     /locus_tag="ACHA25_002198"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8763477.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(12587..>12821)
                     /locus_tag="ACHA25_002199"
     CDS             complement(12587..>12821)
                     /locus_tag="ACHA25_002199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763478.1"
                     /translation="RTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFLLV
                     YAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         3685 a         2701 c         2219 g         4216 t
ORIGIN      
        1 ttgactacgt aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac
       61 tacatcaaat tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc
      121 tgtacctgtt tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt
      181 atcaacaggc attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc
      241 tttttctatc agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc
      301 gatgtggact gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata
      361 gccaagtata tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt
      421 tgggcgactt cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag
      481 gatatataat gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc
      541 gattgctgct gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc
      601 tgtagctctc aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg
      661 tgcaaatttc aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac
      721 attttctgga tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt
      781 aatgatgatc gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg
      841 ttcctgatct gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc
      901 agtatctgat agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc
      961 gatcattagc ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag
     1021 tactcctttt tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc
     1081 caagtttttt accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt
     1141 gttttcctct cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg
     1201 gtcattattc tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta
     1261 tgattcccgc acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt
     1321 taaataaaag agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc
     1381 ccatgaatcc taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg
     1441 agctgatcgt tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta
     1501 agccaacaag tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa
     1561 atggcgttgt tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa
     1621 tcaccatgcc atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa
     1681 ttgttaaact aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga
     1741 tgatgattgc taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca
     1801 ttttttcacc cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa
     1861 agaccatccg aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg
     1921 caagacttgt ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt
     1981 ttctgtcaaa cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca
     2041 tctggaatac cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg
     2101 ccaccgccta ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca
     2161 tttgattctt tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa
     2221 acaagaatat ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc
     2281 atatctttca cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct
     2341 ttgaatacat caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt
     2401 tgtagctctc ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga
     2461 ggtgatttaa caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg
     2521 tacgcttctt tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca
     2581 gaaacgattt tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt
     2641 tcgattgacc agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac
     2701 aaagccattg cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg
     2761 atagcttccc agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg
     2821 atttgaccaa agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct
     2881 tgatttgcat gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta
     2941 tggtttttga tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct
     3001 tcactcattg gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga
     3061 acatctgtaa caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct
     3121 acttgcccac ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct
     3181 tctgcgactt cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat
     3241 tcttgttctg tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat
     3301 tgtaccccca ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc
     3361 atttcttttt caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc
     3421 gttaattcta ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga
     3481 gtcgtcccgt tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca
     3541 ttgattgttt cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt
     3601 tgttgaagta cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga
     3661 tataagaatg cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa
     3721 cggcgtaaga cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat
     3781 gaaagggcgc gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca
     3841 ccatatttta cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct
     3901 gtctcaaagt tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc
     3961 gtatcaatgt ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct
     4021 gaaaatacta agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca
     4081 ggatcgtctt ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc
     4141 ggaccacatg ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct
     4201 agtggcaggc caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact
     4261 gttacataaa gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat
     4321 gcccagtgga tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca
     4381 aacatcgtat ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat
     4441 ttttgggcat ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc
     4501 aatgttgcaa caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac
     4561 gcactaggtt caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact
     4621 tgtgcactac ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa
     4681 caccatcaca gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc
     4741 attacctctt aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag
     4801 ggagcaattt gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt
     4861 aaaaaacaaa tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc
     4921 aatatctcaa tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata
     4981 agaataaagg aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa
     5041 tagttggagc ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg
     5101 tattagaaca agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa
     5161 cctattatct atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa
     5221 cacctacttg cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt
     5281 taggcggtca gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta
     5341 gttatgtagt agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt
     5401 gcacagaacc ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt
     5461 aaaaacaaga gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat
     5521 ttttcttatt tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt
     5581 tatttttttc tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct
     5641 ttttcttctt cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt
     5701 tggcacgtct attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt
     5761 caaatgagac gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata
     5821 acgtaatggc cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga
     5881 agtctagatc ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc
     5941 ctctagcagc taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca
     6001 tcactcgttt tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt
     6061 ggtttttcaa gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg
     6121 ctaaataagg atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt
     6181 ttgttgaaat cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt
     6241 caatgattgg attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg
     6301 cagaaaaaac taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat
     6361 ttaaaaaacc catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt
     6421 gaatcttcag cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa
     6481 cctgtggctg ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa
     6541 aattcgagac acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga
     6601 tctgtgcagc tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg
     6661 cattgatttt ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag
     6721 attgcccaat gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg
     6781 tgggctgttt gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata
     6841 tatcaaaatt attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata
     6901 acacagacaa agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa
     6961 tcgtgctaac agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca
     7021 aaaatgatag caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa
     7081 tgaatactgc actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca
     7141 actgccccaa ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca
     7201 atgcttttaa agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt
     7261 tgccaagtgt ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta
     7321 ataaaagcca ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa
     7381 tcgccgctac caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc
     7441 gtcgagtcaa tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg
     7501 taggaagcat aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat
     7561 ttgtcactaa atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc
     7621 cacttagctg cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca
     7681 taaaagaaag ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat
     7741 taaaagctgg ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag
     7801 acaagaccaa taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca
     7861 tctttggccc tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa
     7921 aatacgcaca caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa
     7981 atgttccata ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa
     8041 atggaaaaac aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc
     8101 ttgattcttt gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg
     8161 ctattatttc tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg
     8221 ctgaatatgt actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc
     8281 gcttatgtaa gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg
     8341 ggtttcatgc tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa
     8401 atgactccac atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc
     8461 attttcattt ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt
     8521 cagttacgca aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact
     8581 ttgctatttc atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga
     8641 catttcttcc acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt
     8701 ctaataaaat tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca
     8761 gcaagtgtca atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat
     8821 acgataaaag tccctccaat gattgctact agtggaataa acggatacat tggaactttg
     8881 tatggtcgaa acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc
     8941 gtataaaaaa tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc
     9001 atcatcatgc caatagcgat gactagctct aagatacctg cggctacagg aactttagtt
     9061 ttatcatgaa gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca
     9121 taagggagac gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag
     9181 ataccaactg tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt
     9241 gccgcattgc tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt
     9301 actaataaat aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct
     9361 ttcgccggct ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg
     9421 aacatcgttg ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct
     9481 tggatcggaa acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc
     9541 acaaacaaag gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta
     9601 gaacctaaaa aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc
     9661 attgactggg aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct
     9721 gctacatttg ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta
     9781 tttccgtaaa tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc
     9841 agttcagctc ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac
     9901 atatgcaaac tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct
     9961 gcaccgatca ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt
    10021 aattcatttt ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt
    10081 cccctttcat accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt
    10141 tttataccgt ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat
    10201 ctaagtcgta tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat
    10261 ttggccaaga ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat
    10321 ctggtatgat tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata
    10381 tattgaaggc atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg
    10441 catcgatcgc atctggcata tacatcatat ccattgatgt accttgatca atgaaactag
    10501 tgtactttcc ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc
    10561 ctcctggcaa ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc
    10621 catatttcac ataataataa tcacatagta gttctccagc taccttcgtt accccataca
    10681 tggttgtagg acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa
    10741 acgcgccgat tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg
    10801 cattgaccag cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag
    10861 ctgacaaaag agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc
    10921 gcattttttc gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt
    10981 caggcatgcg tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg
    11041 tgatcaactc tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga
    11101 tcctccctga tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt
    11161 tctctaattt taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc
    11221 tattttttga taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg
    11281 catatttctg atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata
    11341 aatcccatgt tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat
    11401 gactggagta ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc
    11461 tttaaaataa ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa
    11521 agtaatcgaa gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa
    11581 tggccgactt ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc
    11641 aacaacacct atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc
    11701 aaaatgcttg acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac
    11761 gtaggtgatc agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc
    11821 tccatccatg gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc
    11881 tatcgcttcc tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat
    11941 ttttgctccg gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa
    12001 gatagcatct tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga
    12061 ttgaaaggcg attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg
    12121 atggattttc agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc
    12181 ggttgcttca atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt
    12241 ggatgctaga ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc
    12301 tagtatgtcg atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa
    12361 atacattgtt agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga
    12421 acataccaaa catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa
    12481 cgcttacact gattatgaga attatttaac cacatttttt atttacagta tctttttact
    12541 ttcaaatgac ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac
    12601 ctgggcagat aaatataaca caaccattga tagaagccct ttcgttaaaa tcaattgtat
    12661 tctgaatatt taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc
    12721 gtttatcagc aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa
    12781 ttcgaaaaaa ggactgtgac aaaagtcttg tcacagtcct a
//
LOCUS       DAYYFD010000080        12733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000080 DAYYFD010000000
VERSION     DAYYFD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.080"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHA25_002200"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACHA25_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8763479.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACHA25_002201"
     CDS             complement(323..892)
                     /locus_tag="ACHA25_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8763480.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACHA25_002202"
     CDS             1078..1980
                     /locus_tag="ACHA25_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8763481.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHA25_002203"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACHA25_002203"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8763482.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHA25_002204"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACHA25_002204"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8763483.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHA25_002205"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACHA25_002205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8763484.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACHA25_002206"
     CDS             8548..8733
                     /locus_tag="ACHA25_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8763485.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACHA25_002207"
     CDS             complement(8795..9991)
                     /locus_tag="ACHA25_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8763486.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACHA25_002208"
     CDS             complement(10161..11597)
                     /locus_tag="ACHA25_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8763487.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACHA25_002209"
     CDS             11890..12699
                     /locus_tag="ACHA25_002209"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8763488.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tga
//
LOCUS       DAYYFD010000081        12612 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000081 DAYYFD010000000
VERSION     DAYYFD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.081"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(271..1254)
                     /locus_tag="ACHA25_002210"
     CDS             complement(271..1254)
                     /locus_tag="ACHA25_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8763489.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1259..1906)
                     /locus_tag="ACHA25_002211"
     CDS             complement(1259..1906)
                     /locus_tag="ACHA25_002211"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8763490.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1991..2623)
                     /locus_tag="ACHA25_002212"
     CDS             complement(1991..2623)
                     /locus_tag="ACHA25_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8763491.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2639..3190)
                     /gene="coaC"
                     /locus_tag="ACHA25_002213"
     CDS             complement(2639..3190)
                     /gene="coaC"
                     /locus_tag="ACHA25_002213"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8763492.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3187..3924)
                     /locus_tag="ACHA25_002214"
     CDS             complement(3187..3924)
                     /locus_tag="ACHA25_002214"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8763493.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3921..4910)
                     /locus_tag="ACHA25_002215"
     CDS             complement(3921..4910)
                     /locus_tag="ACHA25_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8763494.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5168..5710
                     /locus_tag="ACHA25_002216"
     CDS             5168..5710
                     /locus_tag="ACHA25_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8763495.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5727..6812
                     /locus_tag="ACHA25_002217"
     CDS             5727..6812
                     /locus_tag="ACHA25_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763496.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6824..7630
                     /locus_tag="ACHA25_002218"
     CDS             6824..7630
                     /locus_tag="ACHA25_002218"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8763497.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7631..8458
                     /locus_tag="ACHA25_002219"
     CDS             7631..8458
                     /locus_tag="ACHA25_002219"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8763498.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8455..9528
                     /locus_tag="ACHA25_002220"
     CDS             8455..9528
                     /locus_tag="ACHA25_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8763499.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9956..12612
                     /locus_tag="ACHA25_002221"
                     /pseudo
     CDS             9956..12612
                     /locus_tag="ACHA25_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
BASE COUNT         3887 a         2254 c         2587 g         3884 t
ORIGIN      
        1 aaaaacagct tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg
       61 aacaccgttt attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
      121 tctaaacacg tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca
      181 caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct
      241 gtcccagcct cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc
      301 gatgtttttg tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt
      361 acggtcaatg attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa
      421 agtttgtgcg agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag
      481 ttggacacgt ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag
      541 cataccaatc aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta
      601 agaagcgccg atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac
      661 ctcttcttga aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca
      721 aaaggcgagt ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct
      781 agctaattgg atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc
      841 gccttcttgt aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga
      901 gattccagca gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg
      961 catcacagca tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg
     1021 agtaaaagtg gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa
     1081 aatagggtag ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac
     1141 agctgctgta tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc
     1201 aatttgttct aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct
     1261 attcctcctt tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta
     1321 ctagagtcgt acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg
     1381 tagagcgagt gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa
     1441 cttcttcatc gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt
     1501 tcagccattc atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt
     1561 ttgctggttc atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt
     1621 cagctgcgat cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt
     1681 gagagccggc agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca
     1741 cggaccaagt taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg
     1801 aagcaacatc tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca
     1861 ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt
     1921 cgacgtcaaa tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct
     1981 acgatgacta tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata
     2041 ctgctgcagt cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg
     2101 ctagaatagc tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct
     2161 ggaataaaaa gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc
     2221 cagctaagaa taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat
     2281 aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt
     2341 tccagcttcc atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag
     2401 gtgtttggat aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag
     2461 cacctagtct tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg
     2521 cattgattgg accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag
     2581 ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct
     2641 aagcatttct ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta
     2701 aggcaccttt tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat
     2761 agccgacaga tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg
     2821 tgttcatggc aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg
     2881 aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg
     2941 cgatgagaaa caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg
     3001 attcttgcat gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg
     3061 tgataaaatg tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga
     3121 attgacttgt aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga
     3181 tctttttcat tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga
     3241 tttactttga gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc
     3301 gccatggatt tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact
     3361 attttttgcg actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat
     3421 gatttttgct tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa
     3481 aacgagcaca agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc
     3541 attttccgca aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc
     3601 cagctgttct tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat
     3661 aatcgcatcg tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc
     3721 agattcgatc gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt
     3781 agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc
     3841 taatcgtcct gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc
     3901 tccagctgtg attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg
     3961 tgtgttccag caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg
     4021 acgaccagtt catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga
     4081 tctattggta cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc
     4141 aagacagtcg cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta
     4201 taagagatca atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag
     4261 tcgatttgct gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg
     4321 acttgcaaag cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca
     4381 atgattgact ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca
     4441 gaagcaccag taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc
     4501 acacacaaca tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata
     4561 ttttttggta gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct
     4621 ggatgggtga cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt
     4681 ggattgttac tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt
     4741 cgtatcacct gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag
     4801 gcaattttta ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct
     4861 gtatggaaaa caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca
     4921 ctccttttaa aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc
     4981 gtaaatttag acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa
     5041 aagataaaga aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt caataaattt
     5101 cgtatactaa tacgagtggt agtaaacagg aagtttacta ttagaaaact tcgattcgga
     5161 ggaaattatg gaaattggtg agaagttaag aaatttgcgc atccaaaaga atttgacaca
     5221 agaagaacta ggcgaacgca cagatttgtc aaaagggtat atttcccagt tggaacgaga
     5281 tttaagttca ccatcaatgg aaacattctt ttctattctt gaagtcttag gtgtgactcc
     5341 agaagaattt tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa aagaagatca
     5401 tacggtttat ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa
     5461 tgaaaaagaa atggagcctg tcttgctgac ttttgaaggt gcaggagaat ataagacgtt
     5521 tgaaccttca ttatctgaaa cgtttatttt tgtattagaa ggggaattag agttaagctt
     5581 aggagagacg gtctatgtgg caaaagcagg acagtcgatc tattatcaag cgacaaaaca
     5641 acatcaatta aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc
     5701 atatttgtaa tatagggggt aaaaacgtgg ggaaaccaat catttcattt gatcaagtag
     5761 tgaaacgcta tgatgatgaa acagtattaa aaaaagtaag ttttgagatc gaacaaggaa
     5821 aattttatac actgcttggt ccttccggct gtggtaaaac aacgatccta cgtattatag
     5881 ctggattcac cgaagctaca gaaggggaca tctattttga agggaaacgg ttgaatgatt
     5941 tgccagctaa taagcgacaa gtaaatacag tcttccaaga ttatgcactt tttcctcata
     6001 tgaatgtttt tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc aaatcagata
     6061 tcgaaaagaa ggtaaaagat gctttgcgta tggtccagct tcctggatac gaaaaacggg
     6121 agattagtga aatgtctggt ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa
     6181 atgaaccgaa agtattgcta ttagatgaac cgttatcggc gcttgatttg aaactacgta
     6241 cagatatgca gtatgaactg cgggatctgc aacaacgatt aggaatcacg tttatttttg
     6301 ttactcatga ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc atgaataaag
     6361 ggaaaatcgt ccaaagtggt acacccgtag atatttatga tgagccaatc aatcatttcg
     6421 ttgctgattt tgttggggag agcaatatcg tggacggtgt aatgatcgaa gataatctag
     6481 ttgagtttgt tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag
     6541 ttgaagttgt tttacgccca gaggatttga cgatcacgac accagataaa ggaaaactag
     6601 tcgtaacggt cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg
     6661 aacagcaaaa tgaatggatg gtccattcaa cgaaaaaagc taaagaagga agcaaagtag
     6721 gattggcctt tgagccagaa gatatccatg tcatgcgttt caacgagtct gaagaagaat
     6781 tcgatgcacg tcttgacagc tacgaagaat aggggggaca agaatgaaaa caatgcgcag
     6841 gatctattcc gtcccttata ttttgtggct cggtttattc gtgatcgcgc ctgtattaat
     6901 gatcatttat caatcttttt ttgatatgaa tgggcaattc acgctgaata attatctatc
     6961 ttattttacg tcggggacat cgctttctat gacgatcaat tctgtatggt atgcattttt
     7021 gatcacgttt ttcacgctga tcatcagtta cccgactgct tattttttaa caaaactgaa
     7081 gcacaagcaa ttatggctga tgctgattat tttgcctaca tgggtgaatc tgctgcttaa
     7141 agcttacgca tttattggga ttttcagtgt gcacggcagc gtgaatcatt tctttgattt
     7201 tatcgggatc gggccgaaac agatattatt cacagacttc agttttctgt tcgtggcaac
     7261 gtatatcgag attccattta tgattatgcc aattttcaat gcattggaag agatgaatcc
     7321 ttctttgatc agtgctagta gagatttagg cgcgaatagc tttgagacct tccgacgagt
     7381 gatttttccg ttatcattaa atggtgtgaa aagtggtgtc caagcagtat ttattccttc
     7441 tctttcttta ttcatgctga ctcgtctgat tggcggaaat cgggtgatca ctttaggaac
     7501 ggctatcgaa cagcatttct tggttacaca aaactggggg atgggttcta caatcggtgt
     7561 gattttaatc attgctatgt tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg
     7621 tcgctcataa atgaaaaaat ttaaatggtc gtatctttat ttagggatcg tctttttatt
     7681 cctatatgca ccgatcttct atctgatttt ttattccttc aatgaaggag gaacgatgaa
     7741 cagctttaca ggatttactt gggaaaatta tgaagctgtt tttgaagata cacgtttgat
     7801 cattatcgta ttgaatacgt tcatgctggc tttcttatct gcattgatcg ccacgatcat
     7861 cgggacattt ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt
     7921 gttgagcatg aataacatct tattagtctc tcctgatgtt attatcggtg ctagtttctt
     7981 gattttcttt acactactag gattcggatt aggttttcag agtgtattgc tttcccatat
     8041 tgctttcagc attccaatcg tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga
     8101 ttcaatggtc gatgcggcta gagaccttgg tgctaataat ttccaagtag tcaaaaatat
     8161 cattttgcct tatctttctc caggaatcat agctggatat tttatggcat ttacctattc
     8221 tcttgatgat tttgcggtta catttttcgt cacaggaaat ggctttacga cgctctcagt
     8281 agagatctat tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt
     8341 agttttcttg ttctccatga tattggtcat tggatactac tttattagta aagaaaaccg
     8401 tccaaaacgg ctgaagaaaa acagaagagt acgcgagggg gtggcacggc tgcgatgaaa
     8461 aaattacaat ctctgttttt aggtattctc atcattattg cggtgctgct gcttggtgtt
     8521 cgtcaacttg aaaaaacaag tggcatgagt ggagcaaagg tacttaccat ctataactgg
     8581 ggagattata tcgatcctag tttgatcacg aaatttgaaa aagaatatgg atacaaagtg
     8641 aattatgaaa catttgattc taatgaagcg atgttcacaa aaatccagca aggtgggaca
     8701 aactacgaca tcgctatacc aagtgaatat atgatccaaa aaatgatcaa agaaaaacta
     8761 gtacttccat tagatcattc gaagatcaaa ggattaaata atattgatcc acgattttta
     8821 gacttggatt ttgatccaga taatcagtat tcgattccat atttttgggg aactttaggg
     8881 atcgtatata acgataaatt ctttgatgca aatcaaatta agcattggga tgatttgtgg
     8941 aagccggaat taaaagacag tttgatgctg attgacggtg cacgagaagt tatggggctt
     9001 tctttgaata gtttaggcta ttctttgaat agtaagaata tggcacagct gacagaagca
     9061 gcaaataaat tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa
     9121 atgtatatga tcaatgaaga agcctccgtg gcagtgacat tttctggcga agctgccgat
     9181 atgatgtggg aaaatgaaca tttacattat gtgatcccac cagaaggatc caatctttgg
     9241 tttgataata tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc
     9301 aactttatgc tagaaccgga aaatgcggca caaaatgcag agtatattgg ttattctaca
     9361 ccaaatgaag aagcaaaagc gttattgcca aaagacattt cgtcggacga acaattctat
     9421 ccatctgatg acacgatctc ccatcttgaa gtctacaaag atctaggatc gaaataccta
     9481 ggcatctata atgacctatt cttagaattc aagatgtata ggagataatg gcaaaactca
     9541 gtaagaatgg ggacttatca acgttctaaa gagctaaaaa tcgtcctttt tctatctgag
     9601 ttttccaatc ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta ttttattttt
     9661 tctaccacgt ttttctacca gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga
     9721 cgggctagta aatctaagat tgaaaaagtt acagaaggat aaagtaattg gaaactatgc
     9781 aaatatactt aacccttctg taatctatta ataaaactaa tatatgaatc cagttcaata
     9841 tttgtagcta tgaaaaaaca taaaaatcat attagataaa aactaaattg gtagataaat
     9901 tattttgtca gatatctgag gcaaatattc gactatttgt gtttcaaagt aaaaattgga
     9961 atacaaatat agaatagaca tattgcagcc gatagtttat tatgctttta gacacattaa
    10021 actggaggaa aaaatgaaaa agagcgctaa agatcgagtt tttgatgagc tgatacgact
    10081 aacagggaat atgtcttctg tcactgccga gaaaatttcg aagaatctag gtatctctcg
    10141 tcagaatgcc agccattatt tgacgagatt agtggaagat aaaaaggtcg agaaattacc
    10201 gggaaagcct gttttgtgga agcctttaga tgaatatgcg gttgtagata atacggagca
    10261 aataaacgaa gcctttcatt ctgtagttgg tcatgacgga agtctgagag aggtcattca
    10321 aaaatgtata gccgctgtca aatatccgcc taacggactc agtgtattaa taaatggagc
    10381 cacgggtgtt ggaaaaagtt ttttagccac aaaaattttt gaatacgcta tccatgagca
    10441 aattattgaa aaagatgcac catttgctat cttaaactgt gccgattatg cggacaatcc
    10501 cgaactgctt tcggcaacac tgtttggata caagaagggg gcctttacgg gggctgaaaa
    10561 ggataccgat ggtttattgg caacggccaa caacggctat ctttttctcg atgaggtcca
    10621 tcgcctatcg aaagaaaatc aagaaaagct ctttctattt attgatactg gaaactatcg
    10681 tccagtcggt gaaaatgtaa attggcattc tgctaaggtt cgttttattt tcgccacgac
    10741 ggaaaaaggt gaaaattatt tattagacac ctttgatcgg cgtattcaaa ttagtgttat
    10801 gttgccaact tttgaagatc gccccattcg agaacgatta gagctgattc aattattttt
    10861 tcaaaatgaa gcggatgttt tacaaaaaga tattattgtt tcaagagaag ccttgtcttt
    10921 aatgttatct catcccttta gtgggaatat tggtaagctg aagaatatca ttaaaatcag
    10981 ttgtgcggat gtgtatagtc gaacacaaga agaaccatta acgattggga aaaatgagat
    11041 cttgattcag ttaaatatgc tggaatccga agtagaaagc ttgccgattg ctagtttatt
    11101 gataaagaaa aatcaaaaga taaaaacgga tcgaatcaaa ccgtttgatt atgattcgat
    11161 tttggaaaaa attactcagc tgataaatga aggtaatttc aatgaaaatt tttcttcgat
    11221 aaagaatgaa gtacagcatt tggtgcatca cattgataag gattcttttt tatcaatgag
    11281 tagaaaattg atgattcaga tgtttagaaa ggtctgggta tccgttttag gaaaaacgta
    11341 cggattgacg acgagtggac ctgttgcaga aattattagc agatcctatg tcatttcccc
    11401 aagctataag acagacttaa ccgggataaa tcgtacatta gctgcaaatc tacctcggac
    11461 gacttactta tgtaatcagt tcttggagca tttaccaccg ctctcagaaa aggacgagaa
    11521 ctttctaaga ctgcttattg tcgtgacttt atctgattat gtagatgaat ccatcgagct
    11581 gaagggcctg ttgcttgctc acggagatag tacggctagc agcattcaag cagtagtgaa
    11641 tcaattatgc ggcaattatg tttttgaagc gttggatatg ccagtagaga gtaatgtgtc
    11701 agagattgtc gaatcgacaa ggaatttcgt gaaacggcaa gtgaatacag atagtctagt
    11761 gctgatcgta gatatgggct ctttaaatca aatttacaca cagatcaaga acgatttaaa
    11821 aggggagtta cttcttctga ataatttgac gacttccatt gcacttgata ttggcttgaa
    11881 gatgacgatg aatgcgccat ttaaatagat tgcggaggat gcagcagaca agtattcaat
    11941 caatgttcaa tactttgaag gcttttctca aagtagcaac attatcattt cctgtatgtc
    12001 gggtttgggg atttcggaca aattgaaaga tgtttttcgt catcatttat cggatgaatc
    12061 catcgaagtt ttcacgaagg actacagaga actacgtgaa ttaatcgacc aaaatgatga
    12121 aacttatttt gacaagacaa aattggtcat cacgacttcg gatcttccac gttccttttc
    12181 aattcctaat attaatgttt atgacatctt agacggtgat ggagcagcca atttggagca
    12241 tattttgagt cctgaaatga agccggcaga atttaaccgc ttgattcagg agctagtaaa
    12301 attcttttcg ttggaaggaa ttgcggaccg tttgaacttt ctgaacccaa cgatcgtgat
    12361 gagtgaagtg gaaacagtca tttctaaata tgaaaattac tatcatttca ctttaaatgg
    12421 ccgtgtaaaa ctaaatctct atatgcatac ggcattgatg atggaacgtt tattcttggc
    12481 tcggacagaa aaatctgaag aaccgaatta tgatttgaaa ccagatgaag atgaatttta
    12541 ttatgtttct agaagtattt ttcaatcaat ggagatgaaa tataatttta aagtcaatgc
    12601 ttatgagctt tc
//
LOCUS       DAYYFD010000082        12602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000082 DAYYFD010000000
VERSION     DAYYFD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.082"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1955
                     /locus_tag="ACHA25_002222"
     CDS             72..1955
                     /locus_tag="ACHA25_002222"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8763500.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2030..2314)
                     /locus_tag="ACHA25_002223"
     CDS             complement(2030..2314)
                     /locus_tag="ACHA25_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8763501.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2304..3038)
                     /locus_tag="ACHA25_002224"
     CDS             complement(2304..3038)
                     /locus_tag="ACHA25_002224"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8763502.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3167..3790
                     /locus_tag="ACHA25_002225"
     CDS             3167..3790
                     /locus_tag="ACHA25_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8763503.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3982..5103
                     /locus_tag="ACHA25_002226"
     CDS             3982..5103
                     /locus_tag="ACHA25_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8763504.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5110..8238
                     /locus_tag="ACHA25_002227"
     CDS             5110..8238
                     /locus_tag="ACHA25_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8763505.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8257..11451
                     /locus_tag="ACHA25_002228"
     CDS             8257..11451
                     /locus_tag="ACHA25_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8763506.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11597..12304)
                     /locus_tag="ACHA25_002229"
     CDS             complement(11597..12304)
                     /locus_tag="ACHA25_002229"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8763507.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4290 a         2222 c         2648 g         3442 t
ORIGIN      
        1 tctacttgct attttcactt aatacccgta tattacgaat gaaaaaagtt tatgaaaagt
       61 tggaatattg aatggaaaaa caaaaaaggt taaaaaacag ccgctacatc actgggtttg
      121 atgggctccg ttcattagct gtggtgggtg ttatcttcta tcaccttctg ccaactagta
      181 tgaaaggcgg ctatttgggc gttccgattt ttttcgtcgt atctggttat ttgatcacgg
      241 atctgctgcg gcaggaatgg gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca
      301 gaagaatgaa gagactctat ccggcacttg tttttatgct tgtgacagct tcggcttata
      361 tcactttgtt tcagcgaggg ctgttaaaca atcttcgagg agttgtggtc agcagcttac
      421 tgtatgtcaa caactggtgg caaatcaata acggtttatc ttatttcgat cgatttgcta
      481 atgagtcacc gtttacgcat atctggtctt tagcagtaga aggtcaaaac tatttggttt
      541 ggccgatctt gtttgtattg ttgatggtat ttgttcgtaa aagaaaatgg atcttttata
      601 ctgtgttagg tacatccttc ctctccgcca tcttgatgat gatcttgtat acaccaggag
      661 gcgatccaac ccgtgtttat tacggtaccg acactcgtct gttttctata tggttgggaa
      721 gtgcgcttgc gtttgtttgg ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga
      781 aaaatattct gaatatcgct ggaggaatct cgcttttggc actgattctt tgcttcttct
      841 ttttagatga tcattacaat ttcatctatt acggaggaat gctcctagtg agtctgttat
      901 gcgtagtgct agttgcagtg acagcacacc ccggagcaag tttgaatcga tggctgacaa
      961 atccagtatt tacttggatt ggcaaacgaa gctatggcat ttatctgtat cagtttccag
     1021 ttatgatttt ttatgaagca aaggtgactg atattgccga tcatgttttg ttgcatactt
     1081 tagtggaaat cacattgatt ctaatcatca gtgaactttc ttatcgatat gtggagcgtc
     1141 cgctagcacg ttttgattat ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac
     1201 cgatcatcag taagagcaaa ccatgggttg ttccaggcac attacttgta gcaattgcac
     1261 tcgttggttt cgttacggca ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg
     1321 caaaaatcgc tgaaagtaaa aaattagcag aagaaaccag aaacaattca gaaaacacca
     1381 ctagttcggt tgaccaagcg atccttgata agtacgagtt gtccaaggca caagggaata
     1441 aggcacaaaa attagaacta acggcatttg gtgattcagt tatgttggat gctgcaacgg
     1501 atctacaaga actttatcca aaagtagtgg tagatggaga tgtcggacga caattatatg
     1561 ccagtgtacc gtacattgaa aaattaaagg aacaagattt gttgaaagac actgttctga
     1621 tcggactagg aacgaatggc tcattctcgg aagctcagtt tgatagtgtg atggatgcaa
     1681 tcggtgatcg aaaagtatac tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg
     1741 acgtgaatgc gatgcttgaa cagatgacga aaaaatacga caatatgacg ctgattgatt
     1801 ggtacagcta cagtaatgac catagtgact ggttttatga tgatcaagtc catccaaatc
     1861 ctgaagggat gacacattac attcacttag tcagcgaagc cttgctagga ggaagttcaa
     1921 gtacgaacag cagtgagaca aacgtgacag aatgatagct acaaaaaact tcaggcaaaa
     1981 acatttgttt ttgcctgaag tttttttata tttaccgtgt ttttaacatt catatagatt
     2041 caggaagcaa aacggttgag tgtgtctgta aataacgttc tttttgtttt cgggtgagtt
     2101 ttttgaatgc agcttctgct tttgttgctt cgcttcttga agaaaatact tctgcatgga
     2161 tcatcttaac aggtcttctt ttttgaagac gagtatattt agcaccagta ccattgttat
     2221 gttcctctaa tcgtctagaa agatcggttg tatatccacc ataaaatgtc tgatctttac
     2281 agaataatac gtaaaaataa tgttcatttg ccatagagca tctcacgaat ctctgcagtg
     2341 tattctccat ccatattgta agtgatcaat ggtggagcga ctttgaatcc gtctaatttg
     2401 ccttctttga ttccttcaac taacaaaata tttgcctctt ttccaggttt tgggtaaaca
     2461 aattggatcc ttttaggtgc aattcgatag tcttccatga cctgaatgat ttctaagaac
     2521 cgatcaggac gatggaccat agtaaagcgt ccatttgttt ttagtagctt acttgaaata
     2581 tcgatcactt cttctaacgt cgtatgaatc tcatgacggg cgattgccag gtgtttatta
     2641 gggttcgttt tatttgttgg cagccctttg aaataaggtg gattacagac gactaagtca
     2701 caagaattat gtcgaacgac agataagctg tctttcagat cgatggtgtg gacagtcatt
     2761 tgttcattta aatggttaag cgcgatacta cgtcttgcca tgtctgctag acgaggttga
     2821 agttcgatag cgtcgatagc cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca
     2881 tttcctgcac aaaggtcaac aatcttccct tttttaggta cacgggaaaa gtgagcaagc
     2941 agcacagcat ctaaagaaaa agaaaacact ttggaacttt ggataatctt gatatcttct
     3001 gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa
     3061 aatagtcgtc tgtattatac tatttttatt aaaaagaacc aaggtggctt gtcaaacctc
     3121 tattttttat gcatactatg accataagaa gggaaaggaa gagactatgt tttatcgatt
     3181 tatgcgcggg gtagttcgag tactattagc cttagttaat ggaaatgccc gctatgagaa
     3241 taaagaagtt ttaccgcaag atgagaatta catactagtt gctccccatc gtacttggtg
     3301 ggacccatta tatctggcgg tagctgcagc acccaaggaa ttcagcttta tggcaaaaga
     3361 agaactgttc aaaaatccag ttctgcgatt tatattaact cgatccaatg cttttccagt
     3421 taaaagagaa aatccaggac caagcgtcat taaaacacct gtaaagattt tgaaatcgac
     3481 aaatttgagt ttgatcatgt ttcccagtgg gacaagacat gctacagaac ttaaaggcgg
     3541 aatggcattg atttccagaa tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc
     3601 gttgacactt ggggatctgt tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat
     3661 cgacttatca gatatcaaga aaacggataa agaaggtatg gcggagatcg aacgacgaac
     3721 acaagcagct tttgacaaat tggacaaaga agtgaaccct gatttcaaat atgaagtcaa
     3781 aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt gaatctactg tcctgttcag
     3841 ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt ctgccctctt tttgcgttcc
     3901 aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa agtgttataa taattagaag
     3961 gacatttttt gaggtgattg aatgcgtttt ttacatacag ccgattggca tatcggaaaa
     4021 aaactacaag gttatgactt gattgaagac cagcgtcatg tactatcttc tatactagat
     4081 atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg gagacttata tgataggagt
     4141 gtcccagctg ttgatgccgt agaattatac aatgaattga tggtcgattg gaatttaaag
     4201 gaaaagatcc cgatttttgc tatatcagga aatcacgata gcagtacgag actttcagcg
     4261 ggtacgccat ggttcagtta ttcgaactat tttttgcgaa cacagctagc agaagcattt
     4321 gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc cttactttga accaattgca
     4381 gcgagattat attttgaaga tgacaatatc cgaacgatca agcaagcggt agccaaggtg
     4441 atagataaaa tgcaggagct atttgattca gaaaaaaaac aagtattgat tagccatttt
     4501 tttgtaatgg gaagtgtaaa atctgattcg gaaacgaaaa tcgaagtagg cgggttggat
     4561 tcgatccctg gtgaactact tacaccgttt gattatgtag cactaggaca tttgcataat
     4621 caagctgctt tgagacagga taatgctcgc tatagcggtt ccccattgaa attctcttta
     4681 tctgagatca accaaaaaaa aggcgtatgg atcgttgacc tgacaacagg cctggagttg
     4741 aattttaggg agatcgaacc cctgcatgat attgaagaaa taacagcgag tttcaaagaa
     4801 ttgctggatc ctgcctttta tcagtccgtc aatcgggaga attacatcta tatctatctc
     4861 actgatagaa cgatcatccc gaatatgatg aaccagcttc ggcaaatata tcctcgtatt
     4921 ttggggattg aacgggcaaa cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta
     4981 gaaatgaaag atcctcaaaa attagcagaa gcatttttta gtgaaatgac ggaagagcag
     5041 ctaactgcaa accaagaaaa atggctcgga gaaacgttgc aagaaatcat ggaaatggaa
     5101 tgatcaagaa tgaaaccgaa aaaattacaa cttaaaaatt ttggtccttt tatcaatgag
     5161 accgtagatt tttcacgttt gacagaagca ccgctttttt tgatcagcgg gaagacagga
     5221 gctggcaaaa cgacgatttt tgatgggatc accttcgcac tttttggtga aacttccgga
     5281 agacttcgct ctggaaaaga gatgcgttct ttgtttgcta caccagacga agaaacaagc
     5341 gtgacttttt cttttgaaca tcagcagatg agctatgaaa tcgaacgaaa accagaacaa
     5401 gttttagcaa aacgaaaagg aaacggaaca aaaaaacagg cagccaaagt aacgttgacc
     5461 atttttgatg ggaaaggaaa agaaataaaa caaatcacaa aacgtacaga agcagatcag
     5521 ttgatcagag aactgatgaa tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc
     5581 caaggagaat tccgtaattt tttggtttca tcaagcagtg ataaagagac tgttttacgt
     5641 catttattcg gtactcaatt tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa
     5701 caacagcaac aaaaattgga tcatttagag caagagctgc atcttctgca aaaaaggttt
     5761 gttttagtca aagaagagga accagcagca gggtttgaag ccgttttagc ccaatggaca
     5821 aaccatcagg caatactcga aaaacaaatc acggaagaaa aagaagctct aacggaactt
     5881 caggaaaaac aaaagcaact agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt
     5941 tatgaagaaa aaacgatatt aataaacaag caaaaggaat tagctgctca aaaagacgaa
     6001 atagaggaaa agaaacgatg gattgggtat tatgaattta cgcaacgatt agtcgaacca
     6061 atccagcgcc tacaagaaat aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag
     6121 caaacaaaga aactagcgat gactacgcag aattatgaag agtggcaaaa aaatgaagca
     6181 aaccgcgtac gccgacaaac aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag
     6241 atgatggtgc caattgtcga atccttacgt agtaaaaaac aagacgcaga acaattaaca
     6301 atttcattgg ataaagaaga attgaatgcc gagaagctgt atcaaaatct cgctatctat
     6361 caggaaagaa ctcgaattct tcaagaaaat ttactggatc aggaaaaact gcaagaagat
     6421 cgtcttgctt ttggacagtt aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa
     6481 aaagcaaaag acactgggtc gctcgcacag ataagagaag aactcaaaaa agcaactgaa
     6541 gaaataacaa aaaatagtca gctattgaaa gaacagacag aacaagcaga acagctgaaa
     6601 agccaatggg caaagcagca aattgctcgt cttcagcttt ttttgcgtcc aggggagcca
     6661 tgtcttgttt gtggatcaac ggaacacccc atacaacaat taaaacacga acagccaaat
     6721 aaggaagata tcttaaaagt agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga
     6781 actcagcaaa tcattgccaa aagtgaagca catgaaaata atcttcaaca acaattatca
     6841 gatttagaac aaaaaatcat ggcaacgaat atacaattgg ctgaacaaga agaacacttg
     6901 caacaggctt tgaaaaaggc atttcctgag ataacagaac aaaaaacaaa aacaactctt
     6961 gtagtaatcg aacaatcttt agatagacga aaaagcaaga tggccaatgc aagaatagag
     7021 ctggaagaaa taagacaaca agcagaaaag ctcgaacaac aattagctgt ctacaaacag
     7081 cagcagacag aaaaaaataa tcagctccag caactgcaag gagaaatcaa aagtttagaa
     7141 caacaattgc aagatacgga tagtaactgt ttagaagagg agatacgacg acttgaaaaa
     7201 cagttagaag aagactcgaa tcaactagct gttgagcaga aagcaggagt gcaattgttg
     7261 caagatcttc atacgcttcg gacacagctt gatcttcaac atgaacaaat agaaaaaata
     7321 gatgaaaaaa tagaaaagga acaagaaaga atagctaaaa agttagaaag ccagtcgtat
     7381 ttttcaacaa ttgaagaaat ctgtgcattt gcacaacagc aagcacaata taaagaaaac
     7441 caacgtacag ttacttctta tcatgaagag caattagtga cagctgaccg gctgcgtcaa
     7501 ttagaaaata tccagctaac tgagacattc ccagatacag aagagatcag ccaacagtta
     7561 gaagaaataa gacgtatgat cgaaataaag caagaaaaga tatacggttt acaagaacaa
     7621 aaaattagta atcaaaaaat ctacgatgaa tgtctttcga tttatcacac aagccagacg
     7681 aaattagatg agcttacgca attacagcag ctttcgcaaa cattcaacgg agaaaatcct
     7741 aagaagacta gtttggaacg gtatgtgctt caggtttatc ttcaagaagt gttacaagta
     7801 gcaaatgatc atctccaacg tttaacgaaa aatcgttatc aatttgagct agcggatacc
     7861 attggcagtt atcgagggaa aaccggatta gaaatcaata tctatgatga tcaggcaggt
     7921 atgtctcgaa gtgcgcatac cctgtctggc ggtgagagtt ttattgcagc actttcttta
     7981 gcattagctc ttgccgatgt gatccagaca caagctggtg gtgtagcaat cgatgcattg
     8041 tttattgatg aaggatttgg gtcattagat gaagaagctt tggaaatggc aatggaagct
     8101 ttagaaacga tcgaaagtga aggaagaatg atcggcatca ttagtcatgt aagagaattg
     8161 aaagaacgag tattacaaca aataagaatt gatacagaag gtagcggaca aagcaaaata
     8221 cgctatcaac ttggataaaa agggagattg gtaactatga aatggagtat tccagaaaaa
     8281 gtcattgcac gtggacgtca atacttgcaa gaaggccgag tgttatcggt tgtccctgac
     8341 caagaagaaa acgtgtggca tgcagaagtc ttgggcagtg aattgtatat ggttgatttg
     8401 gatggtacag caaaagagat agattactgc caatgtccct attgggagga acataaatat
     8461 tgtaagcata cagttgctgt ggaactttat ttacggtcga aaaatctatc tcgtatcatg
     8521 aaaaaagacc agccaatgct agaaccggta aaagcaacaa gtgagggaga aatcattgca
     8581 aaaggttttt ctcgtctaaa aacacaaaac aatcctcaag cggtgcaacc tttgattatt
     8641 gaataccaag tagaaacgat tgagactaat cagtatcacc cagagctttc tattttggca
     8701 ctttacttgc gtatcggttc attggcatca ccgaaaaaaa catatatcgt aaaaaacatc
     8761 tatgattttt tacaagctta cacagaaaaa gaaacttaca ctgttaataa acagtaccag
     8821 tttcgtctag ataaacgggc gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca
     8881 ggaagtgctc agacgcatca attattaggt acgaacggct tacaagtcaa aggaaaacta
     8941 gataaaagat atctgcttct cccagtagaa caagcccgct tcctgttaga gcagatgaat
     9001 cagactacac gtttgcatct gcagataaat gaacaaaacg atcaaggaat acaattttcc
     9061 gatcaaaaca aacctttatc attcaaagtg gaaaaatttg gagaagatta tttgctgcaa
     9121 gcattgaatg attttgattt tttctttatg cattatcagt ggggagttat ccaagggaca
     9181 ttttattctc tgacgaaata tcaacaaacg gtttatcaaa cttggaaaca attgattcgc
     9241 cgattggaga aaccagaagt agtgttcaag aaaaaagaat taccgtcttt gtttaaagag
     9301 attatccctc tgctgtcaga gatcggcgac gtagctattg cccctgcagt agaagaggaa
     9361 gtagcagata tccccgtcga atttattttc tttttccgaa aagcaaaagg aaaaatccaa
     9421 gctcgagtcg atttcgtcta tggcgaagtc atctattcga ctgacccgaa gcatgaggtc
     9481 tcgacagaac aggcttttca ggtgttacgt gacacagtag aagagaaacg agtacttgat
     9541 ctattcagaa tgtatcgcta ccagaaaaat gaaactggtt atgaacgtcc attgccaagc
     9601 ggggaagaac tatatgcctt tttccgcaca gaactatctg tcttccgtca gtttggtgaa
     9661 gtcagacttg gaagaaaatt gcgagagttg tatttagatg cgcataaaca tcaaccgaaa
     9721 atcgaagtaa ccgagaccgg ttcatggctc gatattcgat tcgatgtgac agggatagag
     9781 gagcaagaaa tcgatcatgt cctgcaaagc ttgctgagaa atgatgcttt ttatacatta
     9841 gaaaatggtc aggttctttc ttttgattca gaagaatttc aacaaacaag ccaagtgttg
     9901 caacaattac gggaatcgat acgtacagaa gaaggaacga tccatgtacc taaaaatcaa
     9961 ggattgatca ttcaaaatca attggaaaaa agcaatgcta cttttagtga atcgttccaa
    10021 acgatggtac aagatctcat tcatccagaa agataccagg cacagcttcc caaaggattg
    10081 aatgcaacga tgagagatta tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat
    10141 tatcagtttg gcggtatttt agccgatgaa atgggacttg ggaaaacact acagacaatt
    10201 gcctttttac tttcggaaaa agaagaacgg aaaagcttct ctgctttgat cgtagcgcct
    10261 gcgagcttaa tttacaactg gcaggcagag gtgagaaaat tcgccccttc tttaagtatc
    10321 caagtaatca atgggaataa aaaagaacgt gaagaattac tggctaaaga cacagatatc
    10381 cgagtgactt cttatgccag cttaagacag gatctagcta attatcagtc gcaaaaaatt
    10441 gattatttga ttcttgatga ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa
    10501 gcattgagag aattagctgt acctcagcgg tttgcactga gtgggacacc aatcgagaat
    10561 aatttagaag aattgtggtc attgtttgca acgattatgc caggattttt cccgactaaa
    10621 acaaaattca gggaacttgc cgcagaagaa attgcgcaaa tgattcgacc gtttatttta
    10681 cggcgagata aacaaactgt gttgaaagac ttgccggaaa aaacagagat gaatctatat
    10741 tctgcactta cagaagaaca aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa
    10801 gaaatcacat caatggattc tgaagcattc aagaaaaacc ggatcgggat acttgctggt
    10861 ctgacaagac tgcgacaaat ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc
    10921 ggttccggca agctcgaaca agtaaaagat ctactggtag ctgccaaaga gaacaaacga
    10981 agagttttgc tgttttctca atttacaggg atgctgacta tcttgcaaga agagttagcc
    11041 gagctaggga tctcgacatt ttatttaaga ggcagtacga aaccacaaga tcggttaagt
    11101 atggtagacg catttaatgc tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg
    11161 ggaactggat tgaatctcac gggagcagat acggtcatct tgtatgacct ttggtggaat
    11221 cctgccattg aagaacaagc tgctggacgt gcacatcgga tcggacaaaa aaatgtggta
    11281 gaagtgtggc gtatgatctc tgaaggaacg attgaggaac gcatggattc actccagcaa
    11341 gaaaaacgcg aactgttcca aaaagtgatc caaggtaatg aagaacagtt gacgaaactg
    11401 actgaagaag acatccgcat gattttaagt gtaggagaaa tcgaggagta agagactcga
    11461 aaaagcgttc agtaatgggt aagaaagcct ttctaaagaa gcgattgttt atttgattta
    11521 aaaaatagca aggaggctgg gattccattt tatcccagct tccttgctat cattttttta
    11581 tttctatttt taaaggttat acaaagattg tcttcattgt ctctaacagc tgatcaaaag
    11641 aagaaatctc gatatcgcaa attggagtct gttcaatttt gcgttctcga tggttgaacc
    11701 aaagcgcttg ccagttagca ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa
    11761 cgtataaggt ctcttctgga agcatatgaa attctttctc agctagctga aaaatctctt
    11821 tttctggttt ttggaaacct gttgcttgag aaatgagcat attgcgggaa ggaatccaat
    11881 tgttcaattg aagttgcttc agtttttttg tctgatggtc agtcggccca tttgtgataa
    11941 ttcctaatgg gacattcttt tccttcaaga aatcgagggt ttttttgacc tcctcatgca
    12001 agcagatatt atcaagttct tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg
    12061 taatatgagg gtagtctaaa tcttttagtg attggctgat ccgatgtgct cgcatatatt
    12121 ctagtgtcca atctcctgcc attacttttg ggaagttttc atcactatga tggcggaacc
    12181 ggatatatag tggatgcata tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt
    12241 ttcgaaatgg ctgctgctga tcatacatcg tatcatcaac atcaaacaca actgatttaa
    12301 tcaaaatttt acacgccttt cggttttcat tgattttttc acaaatcctt gactattgta
    12361 acagaaaaaa ttgtctaaaa aagctgataa ataaaggttt ttcttactat atttctattt
    12421 ccttgacaga ataaggtttt tatatttcag tttttttcac taaaaatcgt gaaacacgtc
    12481 aataagtttg atgaacaaat taatcattat ggtagaagtc gattgattta ttttaatgca
    12541 atctctgtgg aattttcgaa agatttattt ttttaataca gtctcaatat aactattcaa
    12601 ac
//
LOCUS       DAYYFD010000083        12494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000083 DAYYFD010000000
VERSION     DAYYFD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.083"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..652
                     /locus_tag="ACHA25_002230"
     CDS             <1..652
                     /locus_tag="ACHA25_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8763508.1"
                     /translation="DFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVFESSISTLN
                     DTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSHSTDYHI
                     QLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLAKLGDVV
                     FISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTISEIKE"
     gene            1298..1504
                     /locus_tag="ACHA25_002231"
     CDS             1298..1504
                     /locus_tag="ACHA25_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8763509.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1706..2707)
                     /locus_tag="ACHA25_002232"
     CDS             complement(1706..2707)
                     /locus_tag="ACHA25_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8763510.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2712..4625)
                     /locus_tag="ACHA25_002233"
     CDS             complement(2712..4625)
                     /locus_tag="ACHA25_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8763511.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(4793..5299)
                     /locus_tag="ACHA25_002234"
     CDS             complement(4793..5299)
                     /locus_tag="ACHA25_002234"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8763512.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5459..5899
                     /locus_tag="ACHA25_002235"
     CDS             5459..5899
                     /locus_tag="ACHA25_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8763513.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5925..6953)
                     /locus_tag="ACHA25_002236"
     CDS             complement(5925..6953)
                     /locus_tag="ACHA25_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763514.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(7008..7082)
                     /locus_tag="ACHA25_002237"
                     /pseudo
     CDS             complement(7008..7082)
                     /locus_tag="ACHA25_002237"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7085..7441)
                     /locus_tag="ACHA25_002238"
     CDS             complement(7085..7441)
                     /locus_tag="ACHA25_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763515.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7739..8713)
                     /gene="sgrA"
                     /locus_tag="ACHA25_002239"
     CDS             complement(7739..8713)
                     /gene="sgrA"
                     /locus_tag="ACHA25_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8763516.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8909..9748)
                     /locus_tag="ACHA25_002240"
     CDS             complement(8909..9748)
                     /locus_tag="ACHA25_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8763517.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9933..10820
                     /locus_tag="ACHA25_002241"
     CDS             9933..10820
                     /locus_tag="ACHA25_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8763518.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11414..12108)
                     /locus_tag="ACHA25_002242"
                     /pseudo
     CDS             complement(11414..12108)
                     /locus_tag="ACHA25_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(12092..12490)
                     /locus_tag="ACHA25_002243"
     CDS             complement(12092..12490)
                     /locus_tag="ACHA25_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8763519.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3756 a         2460 c         1891 g         4387 t
ORIGIN      
        1 agattttaaa atggccatgt tgatccaaga aaatgatcta tctgctattt ctatccacga
       61 aaatattcag aaaacagata ctgagctcac tatggcgcaa aaggtgttcg agtctagtat
      121 ttcaacttta aatgacacaa aaaagttgtt acaacaaaag gatctaaaaa aagcggctac
      181 tattatcaat gaatctaaac gtgtctattt tttcggtgtt ggcggttcag agattgtagc
      241 aactgacgct tatcataaat ttttgcgttc ccctatcgca acttctcaca gcacggatta
      301 ccatattcaa ttgatggaag cttctttatt gacggaaaaa gactgtgctg ttttgatttc
      361 tcacacaggg caatcaaaag aaacaatcca tattgctgaa accgtaagaa aaactggagc
      421 aaaaacaatc gttattacta gccaagctaa ttcttccctt gctaaacttg gcgatgtcgt
      481 ttttatttct atttctgaag aaacagaatt ccgatctgaa gctttagctt cccgtatttc
      541 tcaactaagc attttggact ctctatatgt gattttgatg ttttacaacg aaaaagaatc
      601 acgtcgttct atcgctaaag ttcgacatac tatttctgaa atcaaagaat agcaccataa
      661 cagaaaaagc gaaaaatcca taaaacaaaa aaagccacta ccaaatattt ggtagtggct
      721 tttttggttc tgcaagggtg gtacagaaca tcaatagtcg gtgggttaga acgtatgtca
      781 tcgcatcttt ggagaaggtt gtattggtat tattcgagtt acttgttaga tgcgataaca
      841 aagttagtat acagtagtaa aaaatttatc ttttcttttt tttattattt ttgtttctta
      901 ttttctaaaa aattaaaaaa aattttggtg aattaagtat ttccttgata acactggctt
      961 ttggcctcat tttaggttat catttgtaat tttatttcac agttgatatg attgttatgt
     1021 aaaataaaaa agtttaggat acatcgaatt tttggggaaa ttcttttggg ggtatcctaa
     1081 atcttttcta ttatattccc atattttcta ttattggtct agacgtatga tggtaaactt
     1141 tgtacctatc tgattgctga atcattaatt gcttatcatt agccatcttt gtttcaagtt
     1201 cgctagatga tcattttatt cacatcctaa caaacttgtc acatttatag attcttaatc
     1261 tatttatcat acaattataa aaaaggaggt gctgcttatg aaagctatcc atatttcttt
     1321 gctttttttc gctagtgcat tgattttatt tgctattcat ctttgttccc agccttatat
     1381 caataacaac ggaatattag tggaaccagc attcttttgc ttaccactcg gctttttgtc
     1441 ccttcttgcc tccgcaggct tcggatttgt tgcttttttt aaacgatcaa aacaacaaat
     1501 ctgacttata aaatttacgt aaaagagatc gtgacaataa tcgtgtgata ttcccaaatt
     1561 tcgaataaaa ggtgcccctt gccatctcta actcctcagt cacaagttac aatattcata
     1621 aaccatgaaa agcttgttta ttgaaattgc tccttgtgac ttggagctaa gtgacagatt
     1681 acaacctctt ttgcatcata agactttact ccttgaaaaa cattactaac ttttgatatg
     1741 tttcttgcgc ttgtttattg ctgaaatcaa actgatattc atggttcaaa acagcatttt
     1801 tctccgcata atctactttt gtgactttca cccccgcttt ctcaaataca gaagttactt
     1861 cttccatttg tttcgtaaac gtatttgtat ttccatccgt taagaatgtg ttttgcgtcc
     1921 aacgatccgc atgtctgacc aaggctaaac ttttgacctt ctctgattgt tcaaagtcct
     1981 tattatcaaa gtaagcccat ccacattttt cataaagaaa gctagagatt ttatcactta
     2041 cttgagtaag tgcagaagga tccaacagac cagacaaaga aataaacttc gtaatctgct
     2101 tgttctccaa agtcggatca agatgatcag cttttttaat ttcttcattt tgaagtgcta
     2161 acaaatattc tccactaatt tgtgcacctg ccgaatttcc accaatcgaa atattcgacc
     2221 aatctgcttg atcttttatc tcttttttag ctgcttgaat ggcttcgttc aattggataa
     2281 ctggtgtagg atgttttgct tccggtgatc gttcataatc tatatttacg atgatttgct
     2341 gcgtgtctga acatagatat tccaaataat cacgacaatc atttttatcc cctcctacat
     2401 atgcgcctcc atgcatccag aaaagaatcg gtaatttttt cttttccact ttcggtgtgt
     2461 agatatccat attattattt ttgtactttg aaggatagga tatttctttt tttatatcga
     2521 tttgttcgat tttttgctgg aaatcatctg gtcgggcata tacttgttct ttttcacttg
     2581 tgtcaaattg ttttctaaca agcaagctaa tcggctttgg tgtgatttga ttaatcaaaa
     2641 atacagtgac taaaataact acgatgataa ctggtactgc aatcactatt cgcttgaacc
     2701 atttcattca tttaccctct ctttcttcgc caaatccatg tgataatcgt gctaacagat
     2761 aaaaatccac ctgaataaat gagaagcgaa tgtttctttt ctccagtttt cggcaacatt
     2821 cttttttttg catctgtctt tttttgtact ttcactgtca tctgaatcgg ttgcgtatca
     2881 tcacttgatt gatcctctcg aggttcattc tttcccttat tgtctgcatt ctctgtcgga
     2941 tcttgctttt ttggtggagc tactgggatt tttttgtaat agaaaataat agttggtatc
     3001 gtctcattaa aaatcccttc agtcggtcct tcaacatttg ctaattcgta tccttcgaat
     3061 gtcttttgct ctgtcatata attttctcca taagctccgt aaagctggat ttcgtcctgg
     3121 tctttgagta gacttttatt gttttcatcc acatacttta cgtttatctg atcatggaac
     3181 actactttca aaaagtccgt tacatgtttc gtgtaggcag catggtcaac ttcctgtgcc
     3241 tttccatgcc ctgctccttc tactaataaa agatctttat accctcctga taactcatat
     3301 aatttttgcg cgttagacac aggtactaca tcatctgctg ttccatggat aatcaattta
     3361 ggcaatgaat ctgacattcc aatcgtaggt agagcagctt ttgtatccaa atgcatctcc
     3421 tgatcaaaat agtattgttt caaaaggccc ataaataaat cttcatgctc ttgatcccca
     3481 atgatgtcta actgattttt tatggcatcc ggtagtgcgg gggctactgt ctggctgtac
     3541 gtaaaacgta gttgttcatc aattgatgca aatccgcaat tttcaataat tcctttgaca
     3601 ttgacaggaa tatctttcgc taatacagac atcatcgtag ctgctcccat cgaaccacca
     3661 taaagtaaaa tcttttgaga tggccgctct cgaacttctt gatttatcca gtaaaggacg
     3721 tcatctgact catattgtcc ataggtcaca taatcgcctc cgctattacc agtcgctctg
     3781 gaatcaacaa ataataaatt atacccggcc ttataaaaat cattgtattc ttctgtaacg
     3841 atcccgttat tccaattccc tcgaaatccg ccatggatca tgactgtttt atctgaacct
     3901 tgatcgacat aatatgcata tacatccatc attttgccat cttgtttgtt tagtacagtt
     3961 tgatgaagtt gttttgtatt tggatagaag cctgtgtttt ctgtaggcgt tgtcccatca
     4021 tctgtacctc caagcatcga catcaacaca ctgttttttg caaaagcgat atcgtacaat
     4081 agataagtag caatcagatg tttgataatc tctttatcta caccataatc attaacagca
     4141 agctcagtga ctaataactc tattaatttt gacttgatgt cttctgttgg tccattaagt
     4201 atttctgtca tctgtttacc gaagtaattc aaatattctt taccattgaa attttcacct
     4261 ttaccgatat catcattttc tgtcccattc tctttgtcac ttactttagc agaagaggat
     4321 tcgtcgtttg acatttttgt agctgttgct tcttctgttt gttggttatc tgtggtagcg
     4381 gtcgtttctt gatctgtgct agtaacagat gtctcaaccg ttgtttcagt atttgtttct
     4441 gtttgtgaag tactcgattt cgtttcattt ccagcatcag tagtttctgt ggtttctacc
     4501 tcccgagatt ccgattcgga agaaatgctc tctccatata ctgtccattg cgggacgctt
     4561 gtacttacag caaataagat gaccaacgct ccctttaata cttgttttgt tctcaagcta
     4621 ttcactcctt tttatgtttc taggagaatt atatgtaaac gcaaacattt tttatacaaa
     4681 aaataaatgg tttatcccat aaaaatagaa caggattatt ttctcatttt acgtaataaa
     4741 gcagaatgga taaaccagta tccattctgc tttttttaaa cgaagaaaca acttattgct
     4801 ccaaaaatgt cagaaatcgt ttatcccaaa ttttctcgta aaaagcgatt gtttcctttg
     4861 ctttcataaa aggattatca taagttgtcg ttgctcctct acacatcata agctggtatc
     4921 ctaatccatg agcaaacttc acagtcgtat ctatacaata ctctgcttgt gccccacata
     4981 attcgatcga atgacagtcc aattcagtta ataaaacact caaattagtc ttataaaatg
     5041 aattcgcatg ggtttttctc acaaaataac tgtgctcagg aatagataat tccgacaata
     5101 gctcccatgg ttttgtttgt ggcactaatt gagcatcttc gtgttgaacg aaaataactg
     5161 gtttcccaat cgtttcgtat gttttgattc ttttattcac taatgctgtc actgtttcta
     5221 attcgtgtat cggcgaagaa tcaaaacata cccctttttg taaatcgatc actactattg
     5281 cgtcggctgt gtttgacata cttgtgcctt ctttcttttt tcttgattta atagtatttc
     5341 ttatctagct agttccatta tacgtgacaa cattttctta ttttacacct ctaaatggca
     5401 cttttcgact atttcttcat gttattattt cataaatact tagctattgg aggaatgtat
     5461 gatgattaaa ttgtatacag gttctggctg ctcgtcttca agacgcgcaa aacgttggct
     5521 tatcgtccat gatataccgt ttatcgaaca gaacttaatt cgtcaaccac taaccaaaaa
     5581 tgaatttatc cacttactaa aattgacaaa caacggcgtg accgaaatgc tctctactca
     5641 ttctattagt tataaaaatc ttgttcacag aatagatgaa ctcagtttga gtgaactata
     5701 tattgagatt cgtacatccc cttctttatt gaaaaaaccg attatctacg accaatctgc
     5761 aaaacttttg actggattta gcgaagataa aatcaggatg ttcctttcta aaactaaaag
     5821 aaagcaagag ctcaacgaac tcttgctttc tggtcatctt gtcgatttta aagaaaataa
     5881 tctttttgca atgtcttaat tttggcataa acaatctgct tttcttaatt aacgaaatcc
     5941 aatttttcca atatggatct atttttctca ttcctgattt ttatgatttc agctttcaga
     6001 cgcgcccaat cattttttgt aggttgtcca ttccagtaaa atatctgtgt attcgtttga
     6061 tcatatgcct ttttgtcgat aggataatcg ctaatgacaa tatcaggtaa ctgatcagcg
     6121 gatgcactat attttattgg gcgttcagtc atagataata tccttttctt tgcccataat
     6181 tcctgtgttt taccatactt tgattgaata tagaatatga caggttgttc gtgttttata
     6241 aaaatatccc tgactaacat acagtattga aaaatcaacc gtctgttctt ttcataataa
     6301 gtattaagct ctggaattct gcgagctacc ccactaaaaa actgttgcat gattggatac
     6361 atcactggaa atatatgctg aaaatctctg tcttctgctg attttccaaa agcatccacc
     6421 ttcttttttg tcccaaaaac ccttgaatac aaatggatca ttgccaaatt gacatataga
     6481 taattttttt ctgaagttgt aaaacaaatc ttgaagtttt tttcaaaatt ttctacaata
     6541 gacgattcaa tccagcccca actttcttgt actttatctg gagtatccaa caaatcttct
     6601 tccaaatagg tgttcattac accaaacata aaataaaggt aagcttgctc tgttggagtg
     6661 atgtgattca tcgaccagaa gtttgcttca aaaaaaggct ctagtatgta atgcgtaaac
     6721 tcttcttttg ttatcaatgg atgcacaaat ttgttcatct cttctgggac atacgtaatc
     6781 gagtggcctt gtttcattcg ttgcaacgaa attcttagta ggctgatcca tttctttcta
     6841 tctatactac gcgtatatgg cgcatgtttc gcaaattttt ctaatagttg acgtttattt
     6901 tttatacttg ataatatatc agaatgattt tcgtcgaatg actgccaata catcaagtga
     6961 aaaaagtatc ggatacggta ttcttctcct tctatcggat tacctttttt caagctgcaa
     7021 gataaatgat agtttttcaa aatagtatta agttctctta attttctttg aatcgtacgc
     7081 aaacttatat aattttttga ctaagatttt ctaaggattg ataagtacct ttgtaaagtg
     7141 aatccagtaa aatataacca atcgttccct ctaaaagtat agaataaact ttactcagta
     7201 aaaactgaga agaaaactcg acttttatca tcttgttttc tgaaagaaaa gataatttat
     7261 attctggata cctttcgcaa aacgattctg cttgttgaat caccgaattc atcttagcca
     7321 gggtgtagtt tagatctaaa gcgatatcct tcaccacata taatttctcg tctctttcat
     7381 aacaatagtt caaaaattga tatacttgcc tttcattgat atcaataaat tcttcaaaca
     7441 aagtacatac tcctttttat ctattattta gacttattcc gacagctaca tgaataacgt
     7501 acaaatagat tcgttactga tttttcagag atactaccat gcgcagtgtg cattgtcaat
     7561 tattcttgct cttattttcc atttgtgttc ctgttatttc ctgtttctca aatatgttag
     7621 taaacaaaaa tagatctggc aaagctacaa cttcgccagg caatacagca ctctcgaatg
     7681 aaatattcac tcaaaaatct atcgttttat tcaattttag atctgttgct tttattcttt
     7741 atttcactcg atggaagaga gaacgaaaat agcttgctcc tacaatcaaa atgagtaaca
     7801 cacccatcca tgtaattagc ttgttcaagg ttctactacc agtttctggt aaatcgcttt
     7861 ttctattatc tgtttctttc gctttttctt gatgatcaat tccacgagaa acaaaatcag
     7921 gctgttttag cacacttttt aggttgctcc ctgcatttct cttttgttcc ttagttggta
     7981 tgataggtac caaatcaagt tcattattta cttttcctgt gctgctttcg ctgctcgtgc
     8041 ttcctgtgct actctcacta ctcatgcttc ctgtgctact ttcactactc gtactctccg
     8101 tactgctttc gctgctcgtg cttcctgtgc tactttcact actcgtactc tccgtactgc
     8161 tttcgctgct cgtgttttct gtgctaattt cactgctcgt actctctgta ctgctttcac
     8221 cgctcgtgtt ttctgtactg ctttcactac ttgtgctatc cgtactgctt ggcggtccaa
     8281 caactgtcca actgcttggt ttcaaattct ccgtgatcgt catctgtcca tcataaaaaa
     8341 caatcaaatc aactttatct ttactcttga tctcatcaat cgatagttct gcagaaatca
     8401 ttattgtata ctcaatacca gcggtccatt gtacgtcttt taaataaact tccccaataa
     8461 ctttttctga acttccatta ccatcccaac catatgttag aacagcatgt acatcactag
     8521 cattcacgct tttgtttggt ttgactggaa tagtaatgac ttgttgtgtc atgtcataaa
     8581 caatccgatc agttttcaca gtaaatgaaa gattctcatt tgcccgttca tttgcagaag
     8641 tactgattcc aatcattgct gcaattatac tcagcccaag aataacaaat aatacggtcg
     8701 ctgttttctt catttctatc actctccata taattacctt ctatttgata aaggaattat
     8761 ataaaatgaa aatgatgaga acacgacagt cagttgtgaa aatctaacaa aagatgtcgt
     8821 gtattcgctt gaatttgttt actttttcgt ttaatcaatg acagacttaa aaaagcaaga
     8881 ccgatatcag ccttgctttc ttcatctttt attgataaac agcagtgtat ttcttcatcc
     8941 gctttgtttt atatgcatag agtaacaaac caacaatcaa gactaacaag aataatgtag
     9001 ttgatataga tgcttcagcc ccaaagtttc ccccagtaat tattgtattt tgcgattgga
     9061 tcagagtgga gaaaaaacct tgttcttgcg ttccgctgac tggtataccc aaaatagctc
     9121 cttgtgtcca attccaagta atatgagcac caatagccgt aaataaactt cctgtactgt
     9181 agataagtgc gctgaaaaag caacctgcta aaaagatatt gaaaactgcc agaaatgtca
     9241 tgttaggatt caatccgtgc aaaagagcaa ataatgcagc agatccaatc attgctatta
     9301 acggaccaaa tctaagcatc tgatgctgaa aaaagccacg gaataaaagc tcttctgatg
     9361 cccccactgc aataaataaa ataaaactcg ttatcagcgg cacaatgttt gcgtttcctg
     9421 aatctaaccg atacgccccc gtcaaatata atggactagt aataagcaga aacactccaa
     9481 ttccaacaac gagacctccc gctaaatacc cccagtcttt tcctcgaaca gaaacaccat
     9541 aatcttctat tctttttcct tcgaccttac tagcaagaaa taaagctagt aaaaaaacag
     9601 ctaaatgtcc gaccgtaccg gcaattgctc caatccattc actatttcca ataaacatat
     9661 ccgcaagtgc attattgaca agaattccta aaacaaaacc aacaaagact attaaaacag
     9721 atccaatcaa ccgaataatc ctgatcatct ttcatccctc ctttatatta agtttaacaa
     9781 cccttttctt tcttgtctcg tgaaaactat gtaaatgttc tccatcatag agagaataga
     9841 tttttacgaa ctaagaatat ctacagcaac cttacgaaaa tgaaagactg tctccctaat
     9901 tcttctgtta gactgttttt aataaggaaa ggatgggaaa ttttttgaaa aaacaactac
     9961 taattatgtg cctttcagca tgtgcaattt tactattctc aggttgtagc aagtccaatg
    10021 aaacgattgc aacgtttaaa ggtggaacac ttacagttca ggatttttat gataacccaa
    10081 aagttaaatc aaataatcaa acactattaa aaaaaatgat tgtcttcaaa gcatttgatg
    10141 atgtatacgg aaaagagatt tcttcaaagg aagtaacaaa agaatataat gaacaaatca
    10201 aaaaactcgg tccaaactac aaggaacaat taaaagcagt tggccaaaca gaagagacat
    10261 ataagaaact ctttaaacag atgctggctt tccaatacgg tttgaaagcc aatgtaaaat
    10321 taacagataa agatctagac actgcttgga aagaattcta tccagaagtt agtacacaga
    10381 tcattctatt ttcgactaaa gaagaagcag ataaagcaaa aaaagaagca aatgaaggag
    10441 aaaatttctc caagcttgtt caagcttatg gaaaaaataa aacattaaag gaaactgatg
    10501 gaaaaatgaa ttttgattca accaatccag agattcctac tgaagtaaaa aaagcagcat
    10561 ttaaactaaa aaacggggaa gtttctgata tcattcctgt aacagatcca acgacttatc
    10621 agcaaagcta ttatctagta aaaatggtaa aaaaacagga taaaggttct aataaagaca
    10681 aatacaaatc tgagctagaa aaaattgcta ccgaagcaaa aactcttgat acggaattca
    10741 tggataaaac aatccgtaaa gtgatgaaaa aagacaatgt aactattaaa gatccttatg
    10801 taaaaaatat attcaaataa acaaatttga cccaattggg gatgtggcaa aatttgagaa
    10861 gtgatttttg gaaattcctt cttatttttg aggcaaacca cttttgccac aacttcatcg
    10921 gggccttttt ttatttgtac caaaacaaat cactggattt gttacaatca tgaggatata
    10981 atttaaaaaa gttttttatt tctatcacat gaatcaatgc gccattaagt agattctttc
    11041 atgcttccct attttttcac ttgccgtatc gcttcaaaaa caactatcat aaactcaaac
    11101 tcgactgaaa agttatttta aaaaagacta aaaatatccc ttctggactt gttcttctta
    11161 ttctgttata ctagagaaac acgaaagata tttttatgcg acacttttca tttgttttta
    11221 tgaaatttat tcaaaactgg cgtaacttca gagttacaag gatgtattag aggttaggga
    11281 atacatcgtt taaatgagaa ataagtaata gttagtaata gtcacccaaa ctttaaatta
    11341 atgaatattg aatcgtcaga tagcctcctc gagttttcga gaaggctatc gtttatttta
    11401 tggagaaatt gattcagaag taatcagcga ccttttttag ttcaccaatc gtttctgctt
    11461 ggaaagtttg cacccaatct tcttggaagc ccctcactgc tttatcaatc gagggcatct
    11521 ctaatacttg atctagtagc tctttaccta acgttatcgc tgagctccag atcgaatggc
    11581 tgtagtgact tgttcacaat ttttgaaact agctgctaat atttttgttg gtctttgcgt
    11641 acgttcgatc tcagtccgca acgaatcaat cactttcata gcatcaatgc cttgattttg
    11701 catcctgtta taataaggtg caatataatc tgctccatta ttaatagcta aatacccttg
    11761 taattctgtg taaatagctg taccggtaat caaataacct tcttttttca actttttgat
    11821 cgcttgaagg cctgcttgat tcaccggcac cttgataaac gtatcttttc ccagttcttc
    11881 tatgataact cgagcatcct ctatcatttc ttcttccgtt cttcctattg tctgaatatg
    11941 gataggaatc tgtccgatta tttttttgat ttctttcata tgcgagaaga agtttgacgc
    12001 tccttctttt ttcagaattg taggatttgt tgttacgcct gttaaattca aaaactgttg
    12061 acaagaataa atactttttg tatttgctgt atcaagcatg atttccatat agctttcccc
    12121 cattctgatc ctcttgatcg attttaatga ttgtcccctt tcttaaatgc ggcatttcct
    12181 tatcttctac atacattgtt cctggaagtt ccggctcatg agatccatca aattttatcg
    12241 tgatatgtcc taattcattc aaattagttt ggacaacatc tcctactgct gtgattacat
    12301 acatttgttt atcaaaacag agcttcatat cacaagttat ttcttttctt acctgattca
    12361 attcaatctt ataacaataa tctgttaatg tttccggtgc ttcatctcca aataaaatca
    12421 ttattttttc tttcctgaat acttctgctt ctataccaac atctgttaca accgtttcaa
    12481 agatcgtcat ctct
//
LOCUS       DAYYFD010000084        12198 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000084 DAYYFD010000000
VERSION     DAYYFD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.084"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..568)
                     /gene="frr"
                     /locus_tag="ACHA25_002244"
     CDS             complement(11..568)
                     /gene="frr"
                     /locus_tag="ACHA25_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8763520.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(571..1293)
                     /gene="pyrH"
                     /locus_tag="ACHA25_002245"
     CDS             complement(571..1293)
                     /gene="pyrH"
                     /locus_tag="ACHA25_002245"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8763521.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVMGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1429..2310)
                     /gene="tsf"
                     /locus_tag="ACHA25_002246"
     CDS             complement(1429..2310)
                     /gene="tsf"
                     /locus_tag="ACHA25_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8763522.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2409..3191)
                     /gene="rpsB"
                     /locus_tag="ACHA25_002247"
     CDS             complement(2409..3191)
                     /gene="rpsB"
                     /locus_tag="ACHA25_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8763523.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3550..4029
                     /locus_tag="ACHA25_002248"
     CDS             3550..4029
                     /locus_tag="ACHA25_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8763524.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4246..5337
                     /gene="phnW"
                     /locus_tag="ACHA25_002249"
                     /pseudo
     CDS             4246..5337
                     /gene="phnW"
                     /locus_tag="ACHA25_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5330..6115
                     /gene="phnX"
                     /locus_tag="ACHA25_002250"
     CDS             5330..6115
                     /gene="phnX"
                     /locus_tag="ACHA25_002250"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8763525.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6461..8152)
                     /gene="argS"
                     /locus_tag="ACHA25_002251"
     CDS             complement(6461..8152)
                     /gene="argS"
                     /locus_tag="ACHA25_002251"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8763526.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8574..9521)
                     /gene="arcC"
                     /locus_tag="ACHA25_002252"
     CDS             complement(8574..9521)
                     /gene="arcC"
                     /locus_tag="ACHA25_002252"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8763527.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9636..10655)
                     /gene="argF"
                     /locus_tag="ACHA25_002253"
     CDS             complement(9636..10655)
                     /gene="argF"
                     /locus_tag="ACHA25_002253"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8763528.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10746..11975)
                     /gene="arcA"
                     /locus_tag="ACHA25_002254"
     CDS             complement(10746..11975)
                     /gene="arcA"
                     /locus_tag="ACHA25_002254"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8763529.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3349 a         2531 c         2183 g         4135 t
ORIGIN      
        1 tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc ttatctactt
       61 ctttaatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc aaatcatctt
      121 cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca cgacggatgt
      181 tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct aattcttttc
      241 tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca ttggttggac
      301 tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa
      361 aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt tggttcaatg
      421 gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc
      481 ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc attttttctt
      541 ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg ttccgatatt
      601 ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga caagcagtgg
      661 aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta atcctttggc
      721 aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta ctttcggatc
      781 agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg cgccgatttc
      841 tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc
      901 aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa tatatggctc
      961 tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa
     1021 cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt aatcagcttg
     1081 agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc cgccacccat
     1141 aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga tgatttcttt
     1201 gattactggt ggtttgattc caaatcctgc atccccagct aaagcttctc cactcaattt
     1261 taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat tttctattgc
     1321 cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg ttttgcttaa
     1381 aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt attttttcac
     1441 ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt cgccaacttc
     1501 gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt caacagtcat
     1561 atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga atttcttcaa
     1621 gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca atgcttgttc
     1681 tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt cgtttacata
     1741 acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt
     1801 tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc caccaaatgc
     1861 agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt ctccgataac
     1921 ttgagttgct tcgatcaagt catattcaat tgtaccttta tctgttttga ttttcattgc
     1981 agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt ttactaaatc
     2041 ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa cgattgctgc
     2101 agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt catttttctt
     2161 cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca tgtcgccttc
     2221 tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt cgcgtagttc
     2281 ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt
     2341 aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa cagctccatc
     2401 attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat ttcttcgatt
     2461 gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc accttggttt
     2521 ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg aattgcatcg
     2581 tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc aaccatagct
     2641 acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca
     2701 acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa gaatttttca
     2761 agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac ttcaaaagtt
     2821 ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg gatagtatcc
     2881 cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc tgcacgagtt
     2941 gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg
     3001 ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt aactgttttt
     3061 tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt catttttgga
     3121 ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg tttcatagaa
     3181 attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt atcttctacc
     3241 ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg atttgctgat
     3301 gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga gcaaatatac
     3361 ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag aaaaacagta
     3421 gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt ataaaagacg
     3481 aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct ttcgtaaagg
     3541 agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat tgaagaattc
     3601 ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt taccgccacg
     3661 caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat
     3721 ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag gactgaggaa
     3781 gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg agtccacttt
     3841 ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt tttagatgag
     3901 atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat ggtcatctcg
     3961 gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac tgaagtatca
     4021 cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat tttcaatatt
     4081 ttccttatta aataaacctt tacactcttt acaaaaatac atcatttttt attcaatttt
     4141 ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa caagagcagc
     4201 ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta taaattatta
     4261 actcctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt
     4321 acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt attggatctt
     4381 gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcgg ctcctttggg
     4441 gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat ttgttcaaac
     4501 ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt
     4561 tatgaggtat cgtaacatca aatcctccaa gctcatgtga ttacacagct gattgaagag
     4621 aatccctcta tctccgcact agcaatggtc catagcgaga ctacttccgg tattttgaat
     4681 aatttggaag agattgctgc tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg
     4741 atgtcaagct ttggtgggat accgattcgt gtagccgaac tagggattga ttttttgatt
     4801 tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact tgctaataaa
     4861 gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt cacttgatct atatgaccag
     4921 taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac ccatacggtt
     4981 ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga aactcgatat
     5041 tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa tgacagagat ggggttccag
     5101 cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc taaggactgg
     5161 cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa acggatttgt gatctatccc
     5221 gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat ctatccggaa
     5281 gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa tgattaaaac
     5341 tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc ctgtccacgc
     5401 ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc
     5461 gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt ctgtcaaaaa
     5521 acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg aattgtatca
     5581 acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga aacccgacac
     5641 attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca cgactggatt
     5701 tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga
     5761 cttcatggca acttctgaag acgtcgaagg gtatggtcga ccgtttcctt atatgatcca
     5821 taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac
     5881 ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg taattgaagg
     5941 tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga
     6001 tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa
     6061 caatttaaaa gagttaacag aatacatttt gtacgctgac aagaatccag aatgactttt
     6121 atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa gtaataagaa cagccaaaca
     6181 aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
     6241 acaccgttta ttcgaaaaaa aaagggagct gtgacacgac ttttgtcaca gtccccttat
     6301 ttacaaacaa acagtgttta agagacaacc tctccactaa acggtgttca ttcagctgac
     6361 tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt ttgtttctct
     6421 aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta tctggtgcat
     6481 gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa actagagcta
     6541 agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag tatttgttga
     6601 atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat ttttccccag
     6661 cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag ctggtatcgt
     6721 cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc aacaggctga
     6781 ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat cgaacaactt
     6841 cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg aaaatgactg
     6901 ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg tttttctcgc
     6961 tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat aagacgatct
     7021 ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat ggaatatggc
     7081 gcatatcgtc agaccagtca aagcccattt ccttcaagac agctttcaac tgtttaaaat
     7141 gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa gtacgtttac
     7201 ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa
     7261 tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct ttatcctcgt
     7321 tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca ttgtagaaag
     7381 cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta ttgaattctt
     7441 tcatcgattc atcacggaac cattgccata attgaatagc ctcttcatcg ccttcttcca
     7501 gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca gctacttcat
     7561 ggaattggac atataaacga agcaattcat tgattggttc tgctttaaca gcttcttctg
     7621 taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag tcacctaaat
     7681 ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc gaattaccga
     7741 taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga gaagacatgt
     7801 cgattggtac tgttccttga ttcccgatat tgctatcccc ataatgttct ttttctttca
     7861 ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag ttcaagtaag
     7921 gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct aaatctgcag
     7981 cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga aatgcaacat
     8041 ctccgtgttc agctgatttt ggattttcta gtaattgttc gacttgttcc agtgttaaat
     8101 catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc atctttattc
     8161 ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct ctagcgacgg catacttagt
     8221 atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta aaaaaggacc
     8281 agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt
     8341 cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa gaatgataca
     8401 agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta tatcagaaaa
     8461 aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc agatggccta aaggagaaga
     8521 aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac aaattaatca
     8581 gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc taaagaagtg
     8641 atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcctc tactttcgga
     8701 agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt gtttcatttc tgaaacagta
     8761 acagtttcta atttcttttg atctggtttt tggtaattga catacacatt gtctactcct
     8821 gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga agcaaaatct
     8881 ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac aggaatccca
     8941 ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat gacacgtgct
     9001 tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg tccagcatct
     9061 tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc ttccgtgtag
     9121 aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc cacaataact
     9181 tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac ttcgcccatg
     9241 gcattttgta accagtaacc gatagatcct tctgtcattg caacacatgt atctaaaggc
     9301 attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt accgacttgc
     9361 ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt cactaaatac
     9421 tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt agacaagatt
     9481 gcattacccc ctaaagcgac aactactttt cgtttggcca tttcattcgt cctttcttca
     9541 gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc
     9601 tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta
     9661 cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc ttggtcaaat
     9721 tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt gatgccaaag
     9781 cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc
     9841 aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt gatttggtaa
     9901 ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga tacccataca
     9961 tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt aatcatcaat
    10021 tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc ttcaggttgt
    10081 aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt gactaataaa
    10141 gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc ttctactgtt
    10201 ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg ccattcatca
    10261 gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac cattttttga
    10321 ctgaatccac ggaattcgat tccatcaaac atacgaccta gtactttagc tgtatcttct
    10381 gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg atgtgcacct
    10441 agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa
    10501 agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt caagtctttc
    10561 aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc tttttctgct
    10621 aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct ttagattgtt
    10681 tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa
    10741 acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg accaccacgg
    10801 cctcgtgaca attcacttga attgatttct aacactttga tgccatagct acgaagcaat
    10861 tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat tgccaatgtg
    10921 tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt accagttggg
    10981 atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa gttatctgaa
    11041 tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat cttaccgtct
    11101 ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt aaatacggta
    11161 tccaaatgca tcattgcacg gttgtttgga attttgattg ctaacacttt ttcaaatcct
    11221 gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt agttcgttga
    11281 gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc ttcgatatga
    11341 tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct tggatgatgt
    11401 ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt catcttattg
    11461 actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt
    11521 gggtccatgt aaaatggata gtcatcgtct gctgacagat cgtatagact gctagaacgt
    11581 acttcaatat cttttgtacg aaccccagcc atgattttat ctaccatttc ttgcgtttcc
    11641 attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat atgagattca
    11701 tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc ttctgccgct
    11761 aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtctttgc aaagttgtca
    11821 tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg atccataata
    11881 tctggtgtca aattttccac ttcttgtcca ggccttttca gtaaaactgt ctttaatttc
    11941 cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct tctttttgat
    12001 tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc attcaaaatg
    12061 agacattcct cacattaata ttcgataatt aaataattaa acgtttttct atttttaagc
    12121 gtaatttatt cattttttat tctgtataca ctttcaaaaa aatatttatt cattgaaaaa
    12181 aattttttga ataaatta
//
LOCUS       DAYYFD010000085        12135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000085 DAYYFD010000000
VERSION     DAYYFD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.085"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHA25_002255"
     CDS             <1..610
                     /locus_tag="ACHA25_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8763530.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHA25_002256"
     CDS             723..1001
                     /locus_tag="ACHA25_002256"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8763531.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHA25_002257"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHA25_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8763532.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHA25_002258"
     CDS             1458..2213
                     /locus_tag="ACHA25_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8763533.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHA25_002259"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHA25_002259"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8763534.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHA25_002260"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHA25_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8763535.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHA25_002261"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHA25_002261"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8763536.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHA25_002262"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHA25_002262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8763537.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHA25_002263"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHA25_002263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8763538.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHA25_002264"
     CDS             6950..7492
                     /locus_tag="ACHA25_002264"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8763539.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHA25_002265"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHA25_002265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8763540.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHA25_002266"
     CDS             9085..9987
                     /locus_tag="ACHA25_002266"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8763541.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHA25_002267"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHA25_002267"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8763542.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHA25_002268"
     CDS             11430..11852
                     /locus_tag="ACHA25_002268"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8763543.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3268 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatt
//
LOCUS       DAYYFD010000086        12024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000086 DAYYFD010000000
VERSION     DAYYFD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.086"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..934
                     /locus_tag="ACHA25_002269"
     CDS             155..934
                     /locus_tag="ACHA25_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8763544.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            927..1244
                     /locus_tag="ACHA25_002270"
     CDS             927..1244
                     /locus_tag="ACHA25_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8763545.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1488..3098
                     /locus_tag="ACHA25_002271"
     CDS             1488..3098
                     /locus_tag="ACHA25_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8763546.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3255..4103
                     /locus_tag="ACHA25_002272"
     CDS             3255..4103
                     /locus_tag="ACHA25_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8763547.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            4334..4813
                     /locus_tag="ACHA25_002273"
     CDS             4334..4813
                     /locus_tag="ACHA25_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8763548.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            4846..7074
                     /locus_tag="ACHA25_002274"
     CDS             4846..7074
                     /locus_tag="ACHA25_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8763549.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            7203..8846
                     /locus_tag="ACHA25_002275"
     CDS             7203..8846
                     /locus_tag="ACHA25_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8763550.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(8919..9464)
                     /locus_tag="ACHA25_002276"
     CDS             complement(8919..9464)
                     /locus_tag="ACHA25_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8763551.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(9487..9972)
                     /locus_tag="ACHA25_002277"
     CDS             complement(9487..9972)
                     /locus_tag="ACHA25_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8763552.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(9962..10774)
                     /locus_tag="ACHA25_002278"
     CDS             complement(9962..10774)
                     /locus_tag="ACHA25_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8763553.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(10913..>12024)
                     /locus_tag="ACHA25_002279"
     CDS             complement(10913..>12024)
                     /locus_tag="ACHA25_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8763554.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         3887 a         2223 c         2397 g         3517 t
ORIGIN      
        1 cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat
       61 ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac aatgagtccg
      121 ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg
      181 gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag taggattagc
      241 ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac tgttatcgat
      301 tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga cgcaagcatc
      361 atttgcaacg acattattga tggttttctt tttagaatta cgctatacat tattaggagc
      421 aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc
      481 actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc cttcctggaa
      541 taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg gtagcaatat
      601 gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc attttgcttt
      661 gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac tgatctttac
      721 aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa atactttcgg
      781 attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga aggcattcaa
      841 aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc cgctcttcaa
      901 cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt ttagttgctt
      961 gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt actcatcgaa
     1021 aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta tttgcggcgt
     1081 tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg aatatcaaaa
     1141 tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag
     1201 ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt ttattttatt
     1261 aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc atttgcaaca
     1321 gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg gcttatgatg
     1381 caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat tttttgctat
     1441 aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg aataaaaaat
     1501 tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt ttaggggtag
     1561 tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg cttgctacga
     1621 cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt acagtcggat
     1681 ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat aacgctgctt
     1741 gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc gcctctgctg
     1801 cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct aacgttaata
     1861 atgggatact agctatcaga agtttatgcc catctttgat tgctatccca attcttagtg
     1921 caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact tctttgatta
     1981 tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg cgcgtattga
     2041 ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc ttcggtgggt
     2101 tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc gaacttaaag
     2161 attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct gtctctatca
     2221 ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt caaatgatcg
     2281 accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca gatcagcaac
     2341 tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg atcctgatct
     2401 ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg aagaaaaaag
     2461 atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta ttgattcttc
     2521 ttccttcatt gactggaatg tctttattag ctggaccact atacacattg ttttttggct
     2581 atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg tttttctctt
     2641 tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt gcgatccgat
     2701 taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta ggattagttg
     2761 gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt gttcgcggct
     2821 atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc aattttttct
     2881 taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc
     2941 tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt gtggctgtag
     3001 gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt
     3061 tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg ttttttgctg
     3121 tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt tccaatttta
     3181 gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt ctttgaaacg
     3241 aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat ctgccggaac
     3301 gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg gatgggaaaa
     3361 tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta aaaaacagta
     3421 aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta tatgaggaat
     3481 tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg atttctggct
     3541 ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc attccttatg
     3601 actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta gacttaaatg
     3661 atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga gataatacct
     3721 acgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt ttgacaaatg
     3781 gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt tctgaaggga
     3841 aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa
     3901 ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg atacatgcaa
     3961 ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat ccaaatgcag
     4021 aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa aaagccatcg
     4081 gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa aatattccaa
     4141 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
     4201 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
     4261 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
     4321 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
     4381 agactatgat aaagttgatg gaagaaatgc attaaatacg gatgcaaaag ggctttcact
     4441 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
     4501 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
     4561 agcgatctta gctgcacaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
     4621 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
     4681 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
     4741 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
     4801 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
     4861 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
     4921 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
     4981 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
     5041 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     5101 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     5161 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     5221 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     5281 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     5341 gaaatcgttc gagcgattga aaaaggatat ttattagaga ttcaagagcc gactgtcatc
     5401 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     5461 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     5521 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     5581 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     5641 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     5701 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     5761 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     5821 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     5881 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     5941 ggaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     6001 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     6061 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     6121 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     6181 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     6241 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     6301 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     6361 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     6421 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     6481 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     6541 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     6601 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     6661 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     6721 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     6781 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     6841 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     6901 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     6961 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     7021 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     7081 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     7141 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     7201 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     7261 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     7321 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     7381 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     7441 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     7501 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     7561 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     7621 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     7681 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     7741 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     7801 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     7861 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     7921 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     7981 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     8041 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaactt gcattaagcg
     8101 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     8161 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     8221 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     8281 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     8341 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     8401 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     8461 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     8521 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     8581 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     8641 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     8701 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     8761 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     8821 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     8881 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     8941 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     9001 cgaggtctga caggtacttg gacatgtttg atatgcatac cgattgatgt gtcaccgata
     9061 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     9121 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     9181 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     9241 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     9301 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     9361 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     9421 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     9481 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     9541 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     9601 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     9661 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     9721 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     9781 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     9841 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     9901 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     9961 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
    10021 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
    10081 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
    10141 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
    10201 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
    10261 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
    10321 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
    10381 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
    10441 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
    10501 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
    10561 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
    10621 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
    10681 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
    10741 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
    10801 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
    10861 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
    10921 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
    10981 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
    11041 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
    11101 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
    11161 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
    11221 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
    11281 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
    11341 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
    11401 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
    11461 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
    11521 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
    11581 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
    11641 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
    11701 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
    11761 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
    11821 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
    11881 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
    11941 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
    12001 gttccattaa ataaagaata ggaa
//
LOCUS       DAYYFD010000087        11957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000087 DAYYFD010000000
VERSION     DAYYFD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.087"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..2045
                     /locus_tag="ACHA25_002280"
     CDS             27..2045
                     /locus_tag="ACHA25_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8763555.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2115..2321
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2472..4886
                     /gene="leuS"
                     /locus_tag="ACHA25_002281"
     CDS             2472..4886
                     /gene="leuS"
                     /locus_tag="ACHA25_002281"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8763556.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5234..6787
                     /locus_tag="ACHA25_002282"
     CDS             5234..6787
                     /locus_tag="ACHA25_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8763557.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7168..8952)
                     /locus_tag="ACHA25_002283"
     CDS             complement(7168..8952)
                     /locus_tag="ACHA25_002283"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763558.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8967..10688)
                     /locus_tag="ACHA25_002284"
     CDS             complement(8967..10688)
                     /locus_tag="ACHA25_002284"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763559.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10788..11549
                     /locus_tag="ACHA25_002285"
     CDS             10788..11549
                     /locus_tag="ACHA25_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8763560.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3918 a         2161 c         2428 g         3450 t
ORIGIN      
        1 gataaattga agttggagga tagaagatgg aaaacattgc aagaaaagaa cggagacgtt
       61 tagaacagac caaacgtttt cgaaaagcaa aacgaggggc agcaatcgtg ggaactgcga
      121 tggtaggctg ttctgtcgct gccccattag tacagccggt tcctgttcaa gcggatgaaa
      181 cacctgttca gttcggcgca agaatcaata cagcggcatt tatcacagaa attgctactt
      241 atgctcaacc aatcgctcaa gccaacgatc tatatgcatc agtgatgatc gcacaagcag
      301 ttgtagaaag cggttggggc agtagtgcgt tatctcaagc accttattat aatctttttg
      361 ggataaaagg cagctatcaa ggacaaaccg tctacatgga tacgcttgaa tttttaaata
      421 atcaatgggt aaataaaaag gagcctttcc gacaatatcc ttcttttgct gaatctttca
      481 atgacaatgc ctatgtactt agaaatactt catttggcaa cggttattac tatgccggta
      541 cttggaagag caacacgaaa tcctatacag atgcgacagc ttgtttgaca gggcgttatg
      601 cgactgatcc tggttacgca ggcaaattaa ataatatcat cgcaacttac ggattgaccc
      661 aatatgatac accagctagc ggaaacgcag gcggtggagc gactacaggt ggcaataata
      721 ctggaagtgc aggtagtact ggcggttcaa gcaattctgg taatacaaac tcaggttcag
      781 caacttcgac cacttatact gtccaatctg gtgattctgt ttggggaatc tcaaataaat
      841 tcggtatcac aatggatcaa ttgatccaat ggaacaacat ccaaaacaat ttcatttatc
      901 caggacaaaa actgacaatc aaaggtagcc aggaaaatgg ctcttcgacg aataattccg
      961 gtaacaatac caattcttca ggaaatgctg gtacatcaaa cggaggacaa acaactggtg
     1021 cgaaatacac tgttcaatct ggtgattctg tttggaagat ctcaaatgat cacggaatta
     1081 cgatggctca attgattgaa tggaacaata tcaagaacaa tttcgtttat ccaggacaac
     1141 agctaatcgt atcaaatggc ggttcttcta atgctggagc aaatactggc tcaaatactg
     1201 ggaatacaag tggctctagt tcgtccagct caagttcttc taatacagct tcaggaacga
     1261 aatatacagt taaagccgga gactctgttt ggagcgtctc aaataaatac ggaattacga
     1321 tggctcagct aatccaatgg aacaatatcc aaaataactt tatctaccca ggacaacaac
     1381 tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg taatacaggt tcgtcaacca
     1441 atacaaataa cagcagttca gttaatactg gcagcagttc gtctcaaggc acttataccg
     1501 tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa cggcatcacg atggatcagt
     1561 taatcgaatg gaataatatc aaaaacaact tcatttatcc aggacagcaa ttgatcgtta
     1621 aaggtggaac ttcggcaaat acaaatacag gttcaactac ttctgcagcg aaaccaaata
     1681 caccgaatac gtctgctacg acaagtacat catccactgg taatacgatg tataccgtga
     1741 aagcgggcga gtcagtttgg agcgtagcga acaaacatca tatcacgatg gatcagttaa
     1801 tcgaatggaa taatatcaaa aataatttca tctatccagg acagcaagtg attgtgaaga
     1861 aaggttcatc tcaatcaaca aaccaacaat cagttactgg ttcgaaaacc tatactgtga
     1921 aatctggaga atctgtttgg agtgttgctg acagtcatgg aattacaatg gctcaactaa
     1981 tcgaatggaa caatattaag aacaacttta tttatccagg tcaaacgttg attgtagcta
     2041 aataaaatag ctgttcgaag aaataacgat tgaattccac aaaaatattt gttactataa
     2101 ggctacttaa atatgacaac acttgaaaag gaagtagtaa aaagactgaa gtttcagaga
     2161 gcgcatggct ggtgagaatg tgcacggatg cttttgaact cgcctttgag ttgcccttgc
     2221 tgaatacccg agtaagcaag cggcggtgac ggccgttaca acgttcgagg ccttttcact
     2281 aaggtaaaat taggtggtac cgcgtagatt acgtcctata aaaagagcaa tctttttata
     2341 ggattttttt atttcttttg tgaagtttga gaaaacaaag caaaaggaaa taaaaataaa
     2401 cagattctgc cggttttttc gttatattta taatagaaac aagtgttttc aatagaacag
     2461 gaggacagca aatgagttac aatcataaag aaattgagaa aaaatggcaa aaatactggg
     2521 caaaaacgaa tactttcaac acccatgatg atcctgaaaa gccaaaattt tacgcactgg
     2581 atatgtttcc ctatccatcc ggtcaagggc tacatgtagg gcatcctgaa ggttatacgg
     2641 caacggatat cctttcacgt gtgaaaagaa gtcaaggata caacgtgctg catccaatgg
     2701 ggtgggatgc attcggtctg cctgctgaac aatatgcgct agatacagga aacgatccag
     2761 ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca aatcaattct ttaggattta
     2821 gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga gtattataaa tggacacaat
     2881 ggatctttac gaagctttat gaaaaaggtc tagcttatga agcagaagta gcggtaaact
     2941 gggtacctga attaggaaca gttatttcaa atgaagaagt gatcgatggg aaaagtgaac
     3001 gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg gatgctgaaa atcactgctt
     3061 atgctgatcg cttattggat gatctagaat tagttgattg gccagaaagc attaaagaaa
     3121 tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat tgaatttaaa gtggctggta
     3181 cggataaatc ctataccgta tttacgacac gaccagatac tctattcggt gcaacttact
     3241 cggttcttgc acctgaacta gatcttgtta gagaaattac gacccctgaa caaaaagctg
     3301 ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga tctaaagaga acggatcttg
     3361 caaaagaaaa aacaggagta tttactggtg cttatgcaat caatccagtt aatggcaagg
     3421 aaatcccaat ctggatcgcc gattatgtat tatcttcata cggcacagga gccatcatgg
     3481 ctgttccagc acatgatgaa cgtgactttg aattcgctca aactttcgga ctagaaattc
     3541 ttccagttat cgaaggtgga gatgttgaaa aagctgctta cacagaagat ggtacgcaca
     3601 tcaactctga atttctagac ggcatgaaca aacaagaagc aatcgataaa atgaatgaat
     3661 ggctagaaga aaatggcgta gggaaaaaag aagtcagcta tcgcttacgt gactggctat
     3721 tctcccgtca acgttactgg ggtgaaccaa tcccaatcat ccattgggag gacggaacag
     3781 tcacagctgt cccagaagaa gagctaccat tacggttgcc taagacaaaa aacatcaagc
     3841 caagcggtac tggagaatct ccattagcaa atatcgagga gtgggtcaat gtcgttgatc
     3901 ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat gccacaatgg gcaggaagct
     3961 catggtatta cctgcgttat atcgaccctc ataacaaaaa agaactagca gattatgaaa
     4021 agttgaagcg ttggctacca gttgacatct acattggtgg agcagaacat gcggtacttc
     4081 atttactata tgcacgattc tggcataaat tcttatacga tatcggtgtt gttcctacaa
     4141 aagaaccatt ccaaaaacta tacaatcaag gaatgatctt aggcgaaaac aacgaaaaaa
     4201 tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt ggtcgaaact tacggtgcag
     4261 atacactgcg catgtacgaa atgttcatgg ggccattaga tgcttcgatt gcttggagcg
     4321 aaaatggctt agaaggaagc cgtaaattct tggatcgtgt atggcgtttg atcgttgatg
     4381 agaacaataa aatgcgtgac cgtatcacta cattgaatga tggcaagctt gataaagtgt
     4441 atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa tcttcatttc aatacagcaa
     4501 tctcccaatt gatggtcttt atcaatgaag cctataaagt ggacgctctg ccttatgaat
     4561 atatcgaagg atttgttcaa ctactcgcac cgattgctcc tcacatgggt gaagaactat
     4621 gggcaatcct tggaaatgat ggtggtattt cttacgcacc atggccaaca tacgatgaag
     4681 cagcacttgt tgaagacgaa gtagaagttg ttttccaagt aaacggtaaa gttcgtgcga
     4741 aaagcaatgt tccccgagac ttaggaaaag atgagctaga aaaagcagca ttagccaatg
     4801 aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa agttattgcg gtaccaaaca
     4861 agttagtcaa tatcgttgct aattaattgg ctctttgtca ataaggactg atgagttgtg
     4921 caaaatcaaa tctgggccag aatgaacttc attctgtctc agattttttg ttatttcaaa
     4981 ttgattaatt gattgatcaa aaaggttttg attttcatgt tctcaaacac gttcaatgtc
     5041 tttttcttct gaatggtctt gtaagagatc tctgtaatca tcaaattttt gtctattaat
     5101 cttagtattt tttgatgtat aaactacctg aaattgagag tacataccaa attcataaat
     5161 tttttattag tgggactagt gattgtatgg attttgacgc ataatgaatg aagtatgaga
     5221 aaaggagaga caaatggatc aagaactatt tattatcgaa gtattatcag ctaaacaaaa
     5281 aattatcgag aaagcgaaaa aaagagcgaa aagaataatg ttcaagcttt taagcttagt
     5341 atttttctct ggtttgcttt ttttcggagc tgtatcgttc ctatatcgcc atatgagtgc
     5401 agtagtccgt tcagctgaag ggatgcaaaa attaaataat ttgtatcact tttcgtgggg
     5461 agttggcgga ataacaattg ttgtgcttat tgtttttctt ctacttattt ggctccgtta
     5521 ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa agagcacaac agtttttaca
     5581 taataaacca acttctcaag atgatgacta tctttatttt gagaagactt cgacgagtca
     5641 agcttttcgg atacaaaaaa gtgattgcca gttattgctg agatcacttg atgatttacc
     5701 tatctactta ggcgaaagca aatcaaaatt cggaatgaac acattccata tttttttact
     5761 agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct ggtggatacc tgacaactaa
     5821 gatgagtaca ttagtcactg gttttttcct cattctttta gctggaacaa tctattatgg
     5881 gagatacact cagccaaaaa tgtcttctca tataaatgct gcttctcaca tgacaatgaa
     5941 ctcaactaac gatacgcata tacaaaagac aaatgaaggg ttgttagctg atcctaagca
     6001 aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa cttgatacag agacgcactc
     6061 tctctatatg acaacagata gtggtgctaa ttgggtattt gtgccgattg attcgcaatg
     6121 gttgcgtttt ggcgaataca ccctgacaac ggggctgatt cctgaaggtt attggatgga
     6181 caacacatat gatatttccg ccgatttctc atggtttatc tattcttctg gggatgatgt
     6241 acgtctgctg acttcaagag ataatggtaa aacatggcaa acgtctatta tcacccaatc
     6301 agttcagccg atacgttatc gaaaagcggt attttatggc aacgatggtc tatttatctg
     6361 tacatcagcc agtgggatgt ctgcagaaac tttgtacatt tacactacta tggatggcgg
     6421 aatcacttgg ataaaaagtg gaagtacaat gatcaatcag ccagtacaaa atgtcagctt
     6481 cgttacaact tctttaggtt ttgtttcgac acgtgaaagt ttgttttata caaacaacgg
     6541 aggacgctca ttcaaagaat cagttctgtc gattccggaa aactatcaga caggggggat
     6601 agatcttttt caatcaccaa atgaagttac tcaaataagc gcgaatacgt tagagacaaa
     6661 attttatttg ttgaaaaaag gaccaatcga tgcagggaaa atgtttgcct gcctcttccg
     6721 ttcaactgat aacggggaaa catggcaatt cgttcagcaa ctatcgcaag tagaacaagg
     6781 agattaaaga agagagattg tgagcctgac gttcagcacc gagtcacaag aaacaaattc
     6841 aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt gattcggagt tgacaagagc
     6901 aacaagaagc gattttgaag tatagcttct cgtattttac aagatcgtgt cttattgccg
     6961 ataggttgtc tcttgaacac cgtttattcg taaaaaggga ttgtgacaag tcttttgtca
     7021 ctacttaccg aacgaaactg aaggagctat ccaaaaagta tcattagtat tattctttgt
     7081 gtttctagtg gcaccactag tacatttaaa aaagctatcg atcatggact aatcatacat
     7141 gatcgatagc tttttagata atgtttgtta cgctatctga aattgactat tataaagact
     7201 ggcataaagc gttggtgatt ggagtagttc agaatgtgtt cctttttcga cgatatcgcc
     7261 attcttcatt acaaggatca tgtctgcatt ttcaatcgtt gacaaacggt gtgcaatcac
     7321 aaaacttgtc ctatcttttg tcacagcttc cattgcttgc tgaatcaatg cttctgttcg
     7381 tgtatctacg ctagaagttg cttcatctaa aataacgaca ggaggattcg ctagaatgac
     7441 tcgagcaatt gtcaataatt gttgctgtcc ttgagataac gtgccgtttt cgctagaaat
     7501 gatcgtatca tatccgtctg gcagtgttcg aataaagtga tcacattgtg cgatttcagc
     7561 cgcttcgata atttgttctc ttgtggcatc cattttcccg taggcgatat tatcagctac
     7621 cgttccttca aacagccagg tattttgtaa aaccatccca aaagaacgac gtagttctcc
     7681 acgtgaaagc ttggtaatat caagaccgtc gaattcgatc tttcctttat tcagttcata
     7741 aaaacgcatc agcagattga ctaaagtcgt ttttcctgca ccagttggac caacgatcgc
     7801 taccgtttgt tttggcttta ctgaaaaatc gacttgattc atcaatattt tatctgatgt
     7861 gtaaccaaac tgtacatttt tgaaagctat ttttcctttc ggttgttcta aaagtcttgc
     7921 atctggaagg tctggaattt catctgtttc atctaatatc tcaaatatcc tatcgattgc
     7981 tgccattgcg gattggatcg agttgatcac ataagaagcg gtagaaattg gttcagaaat
     8041 ctgattgatg tactgaagat aagcttgtaa gaagccgatc gtcacaccac ctgatagaac
     8101 aagcatagca ccgaataccg cacttatgat gaaggcgagt tgattaatga aacgaatggc
     8161 cggatagata gcacagttca taaattgagc acgtttaaaa gcgttgtatt gctttttatt
     8221 tacttcgtgt atagcagctt ctgcatgagc ttgctgattg aatgttttga ccactagatt
     8281 accagctaag atttcttcca ttcgattatt taaaacaccc agttcatact gattctgttc
     8341 agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca attgaactgc caacgatcag
     8401 caataagacg agcaaagtaa gcttgctttc gatataaaac agcatcagac cagcagcagc
     8461 tattgttacg acagaagtaa aaaattgatt gatacctgtc aataacactt gagaaagttg
     8521 attgagaccg gtagttgtcc gactaatgat gtcacctact tgatgattgt caaaaaacga
     8581 catcggtaat gttttgaatt tttttgtgat ttcttttcgc accctcaatg tgatcttttc
     8641 gcttaacgct gccattgtcc gttcctgaat aaatgaagtg atgctgctta agactgaaca
     8701 gccgatcagc aataagacag ggaacaacaa tgcttgtttg atatcactga aagaaagctg
     8761 tccaatacct gtttttctta tttctgataa gagatcatcg atcccgattc ccatgatcaa
     8821 gggcatggct acgactaata ggttcccgaa taaactgcat agaaacaatc cgtaaaaata
     8881 tttatgttca ggtttgatca ttttccaaaa acgtatcaaa gcacgcgtgt tcaacgtttt
     8941 tttcttttcc atcgtatccc ttctttctat attccttgtg attttgcaaa ctcctgataa
     9001 gtttggcaat tagacagtag ttcttcatga gtaccttgtc caacgatttt tccttcatct
     9061 aaaacaatga tgttatctgc ttgttggatt gtactgaggc gttgagcaac aatcacgaga
     9121 gttttctccg acatgtgttt atgaagggct gctcttaatg ctgcatctgt tttatagtct
     9181 aaagcggaga aactatcatc gaatacatag atctctgctg gtttgatcaa cgctcgagca
     9241 atacatatcc gctgtttctg gccccctgaa taattagaac cgccttgagc aacaaagctg
     9301 tccaatccgt caggaagggt gtccactagt tcttctaatt gagcgatctt gatcgcttta
     9361 gacaactctt ctgttgaggc atattcattt cccatctgca agttgcttaa aattgtcccg
     9421 ctgaataaga aagccttctg agggacataa ctgatgtggg agcggatctg ttcttgagtc
     9481 atttgagtaa gaggaatacc accaagggtt acttttccta tcgtagggtc actaagtctc
     9541 aagagaagtt tcgccaaact actttttcca gatccggtcc cgccaacgat cgctgttgtt
     9601 tttcctttag gtattgtgaa atcaatatct tctagaactg gatcagagat ttcagtatat
     9661 ttaaatgtga catgttcaaa agcagctgca tttgtctgat caaaggttgg ttcagctact
     9721 gttttgatcg tctcttcctc aatttcgatc tctgcttgca gtatttcttc tatccgtcgt
     9781 aatgaggcaa acgatttagg gataatgacc aataccatag ctgcgatcat caagtaagca
     9841 agtgtcagca tagagtattc gactacagca gtcacgacac cgatttgtaa tgctccttct
     9901 gctacaagat ttccgccaaa ccacaaaata gaagcataaa cgattcccat gagcaaaaaa
     9961 gcaattggag tgacccaagc aaatattcgg ttcactttga tcatttgatt agcatagtca
    10021 gcaaagctct tattcgttcg cttttcttca tatgattgat tatcaaaggc acggatcata
    10081 ttaatcccag taaagaattc tctcaatgtg atggtgatcc gatccatttt tggctggata
    10141 ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa caatccatac ataaaaaaca
    10201 acagagatca atgtaaccca tgttagtaat agagaataac gaaacatcat gagaatggtg
    10261 aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa ataaaacaat catttgctgg
    10321 acattatcga catcattcgt cattcttgtc aaaagagatc cagaactaaa acgactgata
    10381 tccttcattg acagctgttg gaatttacgg aaatatttct cccgcgtttg gaatccaaac
    10441 ctagcggaag ccaatgctga aaagtaacta gaggcaactg cagcgattgt tcctagaaac
    10501 gctacaagca gcatttcaac tccaattttt tttataactg aggcttcgcc acttgctata
    10561 cctgtgtcga tcagctttgc tactaatttt ggtacaccta atgtaccggc gatttctaaa
    10621 cagatcatca caaaggtaag gagagcgagt cctttgtttt tctgtaagaa tgtttttaag
    10681 atagacatat tattcctcct tttttcctga tacttttcga ttctatacct tcaagtaact
    10741 tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg agaaaaaatg aaaaaaatgt
    10801 atacgattgg ccaagtatcg gaactatttg atttgccaaa atcaacgatc cgctattggg
    10861 atgaacaagg attaatccgt tcaagtagac aagaagagaa tgattatcga ttgtttgata
    10921 tcgatgatat ttttatgctt tatgatatcg ttttttatag aaaattagat ataccgatca
    10981 aacagatgaa gaatttatat ggaaaaacat tgacggagtt atatgagaca cttgatgaaa
    11041 cagaaagaag aatccataaa gaactggtgg tgatgaaaca aaaacaaaaa gagataaggg
    11101 agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga ggaagaattt cctgtagaag
    11161 agatcccttt tgactgtatg atctcaacgg agtttgaaga tattgtggaa atcaaaaaat
    11221 ttctacctaa ctattcaagc tttgggatga tgagtatgcc agcttccagt tcacagacga
    11281 tgtatggatt ttttatcgac cctagtgaag tacatttatt tactcaagac gtgatttggg
    11341 agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa atccgagatg aaccatgcgg
    11401 aacgtaataa tattttagaa atcagagaaa gaatgtatga aaaaggatgg aaaacaggag
    11461 aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa caatatcaga actgaatatt
    11521 atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt ttaagataac tcgaaaatga
    11581 tggttgtttt agttataaag aaaaattttc tggttcgatt acgggtttaa tgtgggggat
    11641 tattagtcgt ggatagatag aattttagtt ttttagagga aatatgtctt taaaaatgga
    11701 atggttgcaa ttcttagcga tatatacgat gtaattgtcc tcttaaaaaa gtatggaaaa
    11761 ataattgtat tgggtgttat gtataattca tggtgaactg agactaatga gaagaaaaat
    11821 tcatattgtt atgagtgaga ttgtatcaga tgagaatgat aaattaaata ggtgcataaa
    11881 agcattacac ttaaaataca ttgttgctta cattattaca atatgttaat aaggcaaaaa
    11941 taaatagatg gattgaa
//
LOCUS       DAYYFD010000088        11755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000088 DAYYFD010000000
VERSION     DAYYFD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.088"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACHA25_002286"
     CDS             <1..177
                     /locus_tag="ACHA25_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8763561.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACHA25_002287"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACHA25_002287"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8763562.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACHA25_002288"
     CDS             1199..1771
                     /locus_tag="ACHA25_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8763563.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACHA25_002289"
     CDS             complement(2023..2280)
                     /locus_tag="ACHA25_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763564.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACHA25_002290"
     CDS             complement(2406..3221)
                     /locus_tag="ACHA25_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763565.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACHA25_002291"
     CDS             3738..5063
                     /locus_tag="ACHA25_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8763566.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACHA25_002292"
     CDS             5056..5406
                     /locus_tag="ACHA25_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763567.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="ACHA25_002293"
     CDS             5388..5573
                     /locus_tag="ACHA25_002293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763568.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHA25_002294"
     CDS             complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHA25_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8763569.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="ACHA25_002295"
     CDS             complement(6724..7677)
                     /locus_tag="ACHA25_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8763570.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="ACHA25_002296"
     CDS             8289..9782
                     /locus_tag="ACHA25_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8763571.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="ACHA25_002297"
     CDS             9917..10213
                     /locus_tag="ACHA25_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8763572.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="ACHA25_002298"
     CDS             10314..11165
                     /locus_tag="ACHA25_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763573.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11398
                     /locus_tag="ACHA25_002299"
     CDS             11186..11398
                     /locus_tag="ACHA25_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763574.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4041 a         1811 c         2179 g         3724 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaa
//
LOCUS       DAYYFD010000089        11703 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000089 DAYYFD010000000
VERSION     DAYYFD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.089"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..635)
                     /locus_tag="ACHA25_002300"
     CDS             complement(111..635)
                     /locus_tag="ACHA25_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8763575.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACHA25_002301"
     CDS             complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACHA25_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8763576.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACHA25_002302"
     CDS             complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACHA25_002302"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8763577.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2357..3037)
                     /locus_tag="ACHA25_002303"
     CDS             complement(2357..3037)
                     /locus_tag="ACHA25_002303"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8763578.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3163..4032
                     /locus_tag="ACHA25_002304"
     CDS             3163..4032
                     /locus_tag="ACHA25_002304"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8763579.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4058..4573
                     /locus_tag="ACHA25_002305"
     CDS             4058..4573
                     /locus_tag="ACHA25_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8763580.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4620..5183)
                     /locus_tag="ACHA25_002306"
     CDS             complement(4620..5183)
                     /locus_tag="ACHA25_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8763581.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5238..5417)
                     /locus_tag="ACHA25_002307"
     CDS             complement(5238..5417)
                     /locus_tag="ACHA25_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763582.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5494..6003)
                     /locus_tag="ACHA25_002308"
     CDS             complement(5494..6003)
                     /locus_tag="ACHA25_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763583.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6099..6479)
                     /locus_tag="ACHA25_002309"
     CDS             complement(6099..6479)
                     /locus_tag="ACHA25_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8763584.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6809..7558
                     /gene="pnuC"
                     /locus_tag="ACHA25_002310"
     CDS             6809..7558
                     /gene="pnuC"
                     /locus_tag="ACHA25_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8763585.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7742..9019)
                     /locus_tag="ACHA25_002311"
     CDS             complement(7742..9019)
                     /locus_tag="ACHA25_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763586.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9098..9742)
                     /gene="pcp"
                     /locus_tag="ACHA25_002312"
     CDS             complement(9098..9742)
                     /gene="pcp"
                     /locus_tag="ACHA25_002312"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8763587.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9941..10885)
                     /locus_tag="ACHA25_002313"
     CDS             complement(9941..10885)
                     /locus_tag="ACHA25_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8763588.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10887..11573)
                     /locus_tag="ACHA25_002314"
     CDS             complement(10887..11573)
                     /locus_tag="ACHA25_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8763589.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3343 a         2474 c         2050 g         3836 t
ORIGIN      
        1 accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt
       61 tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt ctattcaatc
      121 aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc ccctctaggc
      181 atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct ttcattacga
      241 acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca actgatgatt
      301 tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat actgataccg
      361 acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc tgttccaata
      421 atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta tatataatcc
      481 aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa aactaaaaat
      541 tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc tcctggcact
      601 gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt tttctagaat
      661 tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat accgcttttc
      721 taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca gtgcttcttc
      781 taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt catctaaccc
      841 taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg gaatccgacc
      901 ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg ttatccctaa
      961 tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac cagtcaatat
     1021 cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt ctgtcattgt
     1081 taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt ttgccagtat
     1141 atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc agctgctgtt
     1201 tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt gataatcgat
     1261 aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg tttgttcaag
     1321 ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat tttatagccg
     1381 atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc tacttctgaa
     1441 tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg caattcgccg
     1501 tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac tactttgtct
     1561 acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac ttttggttcg
     1621 atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa ttcttgcgca
     1681 gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc tcttccacgt
     1741 accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat gatctgaagt
     1801 gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc gtctgcaatc
     1861 cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt
     1921 tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc atcccactta
     1981 tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct ggcatgcgct
     2041 gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc ttctgctttc
     2101 aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac gattttgatc
     2161 ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg acctccagac
     2221 gtttttatat gtattatcat acactattcc agaaaaaaat aacagtttat tttatctgta
     2281 tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg cactataaca
     2341 ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt tcttctccta
     2401 acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag ccacgacttg
     2461 cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta tcgatcattt
     2521 tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt tcccgttcat
     2581 ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga tgagaatacg
     2641 cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt ttagcccatg
     2701 acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc tctttataga
     2761 tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat aagccatgag
     2821 cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact tcttcataag
     2881 gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt tgggacgcat
     2941 attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat tctgaggaca
     3001 aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc taataaaaag
     3061 aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac ttgattaata
     3121 tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa attaattgca
     3181 tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga aaatgccgag
     3241 gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg tcgaaactat
     3301 caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat cactttgaac
     3361 ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat tccaaataat
     3421 caagcaatct ctgttttaga tattctagat gctaacggta tttactatga agtagcaacc
     3481 aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc ttccatggtg
     3541 gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc agctaagctg
     3601 gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga aaagaatctg
     3661 gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg tccagttgga
     3721 aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa caatatcgaa
     3781 gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg
     3841 atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag catgctgcaa
     3901 gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa
     3961 tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg cgcgattgca
     4021 gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt tcattcagga
     4081 aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt
     4141 tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg ccatgaatgg
     4201 cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt ttgagctgta
     4261 taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg gtgactttta
     4321 ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc aatatcaaat
     4381 ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt caggagatta
     4441 ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta ttgctgcggt
     4501 tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt cttaccgact
     4561 taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg tacatctttt
     4621 tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg accgacaact
     4681 ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc cacacttaga
     4741 ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa taatcgcgaa
     4801 ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata ccattcgttg
     4861 ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata tttctctaag
     4921 ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc gtaagcaact
     4981 cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta acgataattt
     5041 ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt ctgcttcaaa
     5101 aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc tggagcatct
     5161 tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa catgcagaaa
     5221 aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat gaaataacca
     5281 tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg catttcttta
     5341 tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc gcaattaggg
     5401 catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct
     5461 tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc
     5521 ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt
     5581 ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg
     5641 cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt
     5701 gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca
     5761 aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa
     5821 atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt
     5881 caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag
     5941 tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt
     6001 cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa
     6061 tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc
     6121 aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc
     6181 ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact
     6241 ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta
     6301 gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct
     6361 cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca
     6421 cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata
     6481 tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa
     6541 tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac
     6601 agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga aagtaagaaa
     6661 ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta taattgaaag
     6721 actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca gaacacacta
     6781 catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt tagctcatca
     6841 gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg gctgccaggt
     6901 catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta tcggtaccac
     6961 gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg
     7021 tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact atttaagtat
     7081 cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa gtaaagaatg
     7141 gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt ttatcgcaat
     7201 catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt atacagatga
     7261 tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg gtatcatcag
     7321 tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg ctcaaatggt
     7381 cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc
     7441 tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata accaaaaaga
     7501 acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac acgcataaaa
     7561 aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     7621 cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     7681 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctctattt
     7741 tttatagtaa ttccttcaga tactttcttg cctcatcatt gaaacgcagt gattcttcta
     7801 acttctgata atattccaac gcttgtttct gagcttcagt aaaacgataa tgttttcctt
     7861 tccatttgtc gattgtctct tgcacatttc cagattggat atctttcact actagttgcc
     7921 atttttcttt tgagactgta cggtctttct tatcagcaga ttgtatagct tctcgttctt
     7981 tacgcaatac ttttaaaaaa ccatttctga ctatccgagg aatgctgtcg aagaaccctt
     8041 ctgtaaaacc atagcgacaa tattgcttcc aaataaatag atagattttt ggttggatga
     8101 atcgttggtt tttcgcaaaa tattccatct gagcagtgac ttctggtgtt cgttgacgtt
     8161 ttgtctttct caacacggaa ataaatgttt caaaataagc attaggatgt actggcactt
     8221 cttcatatac gatggattgc caatctttac gtaaaaatgc gcgatagttg atcaagaatt
     8281 cttctacatc ctttactaat tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc
     8341 gcttttttga ctgcccattg atgtgttcac ttattttctt gatttcattt tccaatgctt
     8401 gataatccgc tatgtaaatc ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta
     8461 attttatatc cggtaaatat ggctgtttca tccgacgatt ttggtacaag acaaaacagt
     8521 atttttgcca tagtccttca ggaaaatcga tattcttctg cttaagccat aataattcgt
     8581 ctaaggccag atcaactttt gtcattccct gatggatcga agctgccact gtcggagaga
     8641 catgcaggtg atcggcaaag tggttgatgc ctagttttag gatctttccg gtttttggcg
     8701 atttaacgat gtattgcact tttaattctc taccacactc acaatacaat ttttgctggc
     8761 ttcgttttgt tcgaaaaaac ggattgatct tgaagtcaga aaacacccaa tctgttgcag
     8821 ctaaataatg ttcttccata aaacgagagc tgattagata tcggcggtgc ttatcgacta
     8881 gttcccgctg ttttttcgtt aattttgcat agatctccca acgttgactg cttgtgtatt
     8941 gtttagaaat tttgatggga tagcttttgc tgatttcttt tggaaaccat tcttcaaaga
     9001 cggattgcca ttcttccacg gctgtttctt tcctttctaa ttacaaaaag ctggctacta
     9061 acataacgtt agcaaccagc cagtgtttct tgctaaatta gtgagttgtt ccggcatttc
     9121 cggaaatatc ttctttattt tctactgctg cttcaactgc ataagtcaaa gaagaagcaa
     9181 tgtcttctaa agacatagaa ggcgtaccca ctggacgatc gatcacttga tcgggtgaga
     9241 atggcacgtg gatgaaccca cctcgaattg ccgggaattg tgtttcgatc aaatgaagca
     9301 aacgatacat gatatcattg cagacaaatg ttcctgctgt ataagagatg tacgctggta
     9361 gaccatgatc ttgtacattt tttgtcattg ctttgattgg caatgaggta aagtaagcgg
     9421 ctggaccgtt ttcttctaaa gccgtatcga atggctgatt tccttcatta tcagggattc
     9481 ttgcttctgc tagattgatt gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt
     9541 gtccgacaca gatcacgaca tctggttggt gctgttggat ggcttctttg actacttgac
     9601 tgcttttttc gaatacggtc ggtacttcca ctttgatgat ttcagcaccg ctgatttcat
     9661 ctggcatttt ttttactgct tcataggctg gattcacttt gtccccacca aatggatcaa
     9721 aacctgttac tagtactttc atctgttgtt cctccgtccg attgatttaa catatggtta
     9781 tcttttatca taccattttc cttgaacagc gtaaataaag gaaacaatag tttaaataac
     9841 ataacagatc aaacttaata tattcttata aaaaaatagt gttccactac aatgttagaa
     9901 gaggttgcga caattattct gtcacaaccc ctctcttcta ttatttaaat agaatcatat
     9961 aagcaatttg gaaaactagc ataaataacg caataaacaa ctggtttttg atcactccgt
    10021 atttgtcttt catctccagc atcgctacag gcacgatatt gaagttagca gccatcggtg
    10081 tcagcagggt cccacaaaaa ccgcaagtca atgcaaccat tcctattaac gctggattcg
    10141 ctccatgact aaatacgaat ggtgcaccga tcccaaccgt catcacggtg atggccgcaa
    10201 atgcattccc catgattacc gtaaatatca ccatgccaag agcgtaaatg atgatgccga
    10261 tgttcacatt tccttctgga acgatcgtac ctactgcatt ggaaatcact gtcccaactc
    10321 cagcgcttgt aaataccgca cctagagaag ctaaaagcat tggcaacatg cttaacggcc
    10381 ctactgtgcc cagcatatct gctgcatcat ctaaaaatgt acttggtcga ttatctcgag
    10441 aaaagaacat caagattagc atggctgcaa aaacaccgac gcccacgccg actaaagcac
    10501 ctaaattggt aaatagagca aacaagatcg caaagacacc gatactcaaa gctggaataa
    10561 aaattttcat tcctagtttg tctgacattt tttccatata ttcttttgat ggaagccggc
    10621 tttctccttt gctgactttc ttagcaattg ctggtatggt catcgcaaag atcaagccac
    10681 cgttcacgat tgccggaagc catcttcctc ctgcaataac tacccctaag accgtccaga
    10741 aaaaggctga cccataacga tgaggattgg ttttatctgt taaatttctt actccagtgt
    10801 aaatcaatac gagtcccatc acgatataaa ggatctccag taatttatcg cctaataaaa
    10861 tatcaggatt tgtaaaaaag tccatcttat tgcccctttc ctggataata tttcttcgct
    10921 aatttctttt ctttaagata gaagtagaca cttgccacaa ctaaagccgt gatagccact
    10981 ggaacttcca ctaaagcaag ctgtcccaat gttacgtcat atcctagatc tttcagtgtt
    11041 gattggacta gcaacgcccc tgctcctccg atgaatagta cttggccaaa gaaccagcag
    11101 acattttcca tagctgaagc catacctttt agttcttctt catgcttagg atggacttct
    11161 aacccttttg attcgatggt ccccaatgcc atcggtaaaa tgattggacg aacaaatcca
    11221 gcaactccac caaaactcac gttaaaagcc gcaaagatca ttcgaaagat cccataaata
    11281 tctacaatca taccagcaga tactttcttg aatcgcttga tcaaggaagc cgctgattct
    11341 ttcagaccat ttctttcaag cgttcctgtt gccaacataa tgatgataaa aatcgccatc
    11401 ccgcgattgt ttacaaaact tgtacctaaa agttctaaca ttccttgaat gccaagaccg
    11461 ccagtcaaag ctgtaacaac taaagcaacc attacgatca atatcgagtc taatttaaat
    11521 gcaaacccga gaataacaat caagacgcct aaaaggctaa gataattttc catatatttt
    11581 cctccataaa atgacaatat ttttttaatt ataaagcaaa acacataaaa aatatagttg
    11641 ttttctcacg aaatagacac tatttttaaa tatttttcgt tattttttta tcacaaaaat
    11701 tat
//
LOCUS       DAYYFD010000090        11594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000090 DAYYFD010000000
VERSION     DAYYFD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.090"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA25_002315"
     CDS             6..305
                     /locus_tag="ACHA25_002315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8763590.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA25_002316"
     CDS             378..1739
                     /locus_tag="ACHA25_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8763591.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA25_002317"
     CDS             1761..2021
                     /locus_tag="ACHA25_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763592.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA25_002318"
     CDS             2035..2787
                     /locus_tag="ACHA25_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8763593.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA25_002319"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA25_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8763594.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHA25_002320"
     CDS             complement(3539..4513)
                     /locus_tag="ACHA25_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8763595.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHA25_002321"
     CDS             complement(4891..5610)
                     /locus_tag="ACHA25_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8763596.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA25_002322"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA25_002322"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8763597.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHA25_002323"
     CDS             7161..8558
                     /locus_tag="ACHA25_002323"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8763598.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHA25_002324"
     CDS             8639..9079
                     /locus_tag="ACHA25_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8763599.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHA25_002325"
     CDS             9227..9553
                     /locus_tag="ACHA25_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8763600.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHA25_002326"
     CDS             9554..10303
                     /locus_tag="ACHA25_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8763601.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHA25_002327"
     CDS             complement(10383..10838)
                     /locus_tag="ACHA25_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8763602.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHA25_002328"
     CDS             complement(11265..11540)
                     /locus_tag="ACHA25_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763603.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3657 a         2013 c         2355 g         3569 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttat
//
LOCUS       DAYYFD010000091        11563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000091 DAYYFD010000000
VERSION     DAYYFD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.091"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..120
                     /locus_tag="ACHA25_002329"
     CDS             <1..120
                     /locus_tag="ACHA25_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8763604.1"
                     /translation="PTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(735..1775)
                     /locus_tag="ACHA25_002330"
     CDS             complement(735..1775)
                     /locus_tag="ACHA25_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8763605.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2124..2450)
                     /locus_tag="ACHA25_002331"
     CDS             complement(2124..2450)
                     /locus_tag="ACHA25_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763606.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2437..3225)
                     /locus_tag="ACHA25_002332"
     CDS             complement(2437..3225)
                     /locus_tag="ACHA25_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8763607.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3593..3883)
                     /locus_tag="ACHA25_002333"
     CDS             complement(3593..3883)
                     /locus_tag="ACHA25_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8763608.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4019..4198)
                     /locus_tag="ACHA25_002334"
     CDS             complement(4019..4198)
                     /locus_tag="ACHA25_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763609.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4195..4545)
                     /locus_tag="ACHA25_002335"
     CDS             complement(4195..4545)
                     /locus_tag="ACHA25_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763610.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4538..5863)
                     /locus_tag="ACHA25_002336"
     CDS             complement(4538..5863)
                     /locus_tag="ACHA25_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8763611.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6313..6927
                     /locus_tag="ACHA25_002337"
     CDS             6313..6927
                     /locus_tag="ACHA25_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8763612.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7092..7334)
                     /locus_tag="ACHA25_002338"
                     /pseudo
     CDS             complement(7092..7334)
                     /locus_tag="ACHA25_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7538..8857)
                     /locus_tag="ACHA25_002339"
     CDS             complement(7538..8857)
                     /locus_tag="ACHA25_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8763613.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8991..9599)
                     /locus_tag="ACHA25_002340"
     CDS             complement(8991..9599)
                     /locus_tag="ACHA25_002340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763614.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10237..10494)
                     /locus_tag="ACHA25_002341"
     CDS             complement(10237..10494)
                     /locus_tag="ACHA25_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8763615.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10487..10756)
                     /locus_tag="ACHA25_002342"
     CDS             complement(10487..10756)
                     /locus_tag="ACHA25_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8763616.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10887..11066)
                     /locus_tag="ACHA25_002343"
     CDS             complement(10887..11066)
                     /locus_tag="ACHA25_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763617.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11164..11400
                     /locus_tag="ACHA25_002344"
     CDS             11164..11400
                     /locus_tag="ACHA25_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763618.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3550 a         2091 c         1756 g         4166 t
ORIGIN      
        1 cccacgatta aaggcatgga agccatccga ggattatata agaaaacccg aaaagaaggc
       61 actctcttcg ggttttcggt ctgtactgaa atcaaggtat tattgggaat cccagattaa
      121 atcatagata ccgtaaggga ttttattctt tatttaaaac tttgcaacag aaccgtccgt
      181 tgcgggtaat accgtaacac tgaagggcac gcattaaggc gtcatcttaa tgctttcctg
      241 taggacactg acggagatta ccagtctccc gagccttaca ggggcctttc tttttttgta
      301 ttttcaatta tcacaattta tcgtccaaaa gtgcagaacg aaaatcaatt catcacaagc
      361 atatcagaca attaaattta aaacaactaa aaatatacaa aaaagtgtaa ttgaatatta
      421 gcagcactgc ctgaaaagaa gtaatatcag gcttttcagc ttggggaaat aagtgaatta
      481 cctctcttca tgaattatta taactcaaaa aagcacatat gcaaacacta gttccctatt
      541 tttttgaatg aaaaataact aaaatatcta acgattatga ctttaaaacc tacgtttgcg
      601 attgttttca taaactttta atctaaaaac tccggaataa tagctattta tttgtaacat
      661 ttgtaataaa attaaaatca taaaaaagtt ctaatttaaa attgatcatt tttatagtga
      721 tcgcttttta tcaactactc tagccaattt tccgtactaa ctgttggtaa ttcgttaaat
      781 gtttctcttt gttcgtttgc aagctttgcc gcaatagctt tttcgacaaa ccaatttttt
      841 acataaacga aaattagatt ctgcaaattg tttacttttt cagttttttg tttttgataa
      901 gctttcagca aagtcatata taatcctttg tcagcatcaa ttccataaga ttccagttct
      961 tcataaacaa caactgtttg ctctaattct tcagcttttt tctttgcatt atgaatagtt
     1021 tggacggttt tttgagcgtc ttcgtaagta ctagaaaatg tttttatcaa gtttaataca
     1081 ttttctggta caaatgtatt tatactgtcc tctttcatga tgtcgacgaa gttatctagt
     1141 aaaacttgat tttgtaactg atcagtttca gtgtctatta tgtgtctatt agtgtctata
     1201 gaattagaat agaatagatt gtctgcgatt ttcgcaatta cggttgagtc cttgatatga
     1261 ctggattttg ccgtatttgc gattttcgca attacggtgg tatcattgat ttctcgcggt
     1321 tttgccgtat ttgcgatttt cgcaattacg gtggtatcag tattgtcttc ctcttccatg
     1381 attttaaaga cgtcttgatt cgttacttct ggcttgccta agtacaataa attaggcatg
     1441 gattcaatct taccgtcacg tttatttact cttcctctct tctgtttcag aagacctgct
     1501 ttttctaatt cctttttgat tttagatact ttaccttcgc ggcattgtaa caatttcatt
     1561 aattcttcaa ctgtgaatat gaaaaatata ttatcatcag aatctaccca gttatttttg
     1621 actgaataat caaatcgatc tttaagtagc atataagcaa tttttgcatc atttgataga
     1681 tccatgtatt tttcgttagt aaaaaatacc ttaggcattt gataatattt ttcacgataa
     1741 atttcttttg atttgtaata attaaaatca ttcatagcca atcctctttc tgtaggatag
     1801 cgttttgttc aaaactatgt tacaatgaat taaacaaata tatctatcca ctatatattt
     1861 ttctactgct gataagtcgc caaactttgt taaagcagta gtcttttttt atcttttttt
     1921 agtttacctt aaaaaataaa ttaaagcaaa ccgctctatt tcaaggttta agctcatagt
     1981 aactatatca cgatattaca ataatattat atcacgatat cgtaatatta caatatgaag
     2041 ataagcgact tatcagcttt gatactactg atttttataa aaaaaatatc acgatattat
     2101 gatatcgtga tattgaatgt aaattaaatt ttattttgtt tctttgctag tttatatcct
     2161 tcaatcaagt attcttcagg tatggacttt aatagcgcat cttctataac gtctggttta
     2221 cttttgtctg attcagcaat agtcgtccaa tatttgagtg ctgacattag aattggattt
     2281 atacgaatgc tatctgctgt tcttacacta cttccttgat cttttatgac tttacttata
     2341 tttttcttgg ttaattcgat ttcttctact tcttttgatt taaaattctt tttattttta
     2401 tttcctagta atctacttac tgcagcttga ctgcgattat cttctgccat caaaatcacc
     2461 aacttctttt aaatattctt ctgtaaatgc tttatagtca tcttcagctg ttgagggttt
     2521 attaaaattg attagatctg atccagcagc ttgcgcttcg tcaacggtta ttgtcctacg
     2581 aatataagtt ttgaaaattt ttgcaccaat gactctagaa atttcggttg ctgcagacgt
     2641 catttcttta gaaatgttat aacgcttatt atacttagta aatagaactc ctcgtacttc
     2701 aagcttgtcg ttcgtatatt cttttaccgt ttcaatagtt tcacttaact ttattagtcc
     2761 ttttaatgag aaatacgcag cttcaactgg aatgataatt tcatcagaaa cagtaaatgc
     2821 gttgactgta agcattccta aacttggtgg acaatcaatt ataatataat catagttgtc
     2881 cttgattggt tgaagtactc ttttaagcct gtgttcactt cctacttgag taaattccct
     2941 ctctgcagaa gaaagggcta tgtcggctgg caaaacatca attcctttat agtccagaat
     3001 agcatctgta atgtcagcct ccccttttaa tacttcataa attgtaggtt ggtttgttcc
     3061 atctatatcg aaacctacat tatctgtagc atttccctga ggatcaatgt ccactaaaag
     3121 tacattttta ccgtaaacaa atttcaaaca tgctgctaaa ttaacgctag tcgtagtttt
     3181 agaaactcca cctttttgat tggctactga caatattctt gccattaaaa tatcccccat
     3241 tcatcttata ttataatata attatatcat aatattgtga tattgcaata tcgtatatgc
     3301 attatattgt aatattgtgt gattattttg ttttaaagat atatatcggt tcggatataa
     3361 agattattca tatgttattc gaaattaaaa taatcacttt ttattacttt aaaatttttt
     3421 attaatgata agaaatatgt tttgcgagca gcaactagaa taatttaaac attactaaac
     3481 tgtctatatt gttactaata attcccttct tatagtgcat tttcttagaa aaaaacaata
     3541 ttacgatatc atgatattaa gatatcgtaa tattatacaa ttgtgattga tattatatat
     3601 gtttttttat ttttttatca tgtttaatct tgaaggaatc atattctttt tctaaagaca
     3661 ggagcttttc ataaacatca gacatgttta aactagaggc caggcttaaa tcataaacga
     3721 atgaagcagg taatgatttt attctacgtt gtcgggttat ccagcttgca accgttcctt
     3781 gtgggtaggc atataactca caaaattgtt ctacagtgat tcctaaattc ttgataatgt
     3841 aaacattgat cgggtgcgag taaacaaatg tattgataga catggtgcgt aagcctactt
     3901 tcttttatta ttgcgtttgc aataatagtt taacacacgc attattctag tatctactcc
     3961 tatttgtaaa aacaaaaaag gattcataga gataactcta tagaatccgt aaaatacctt
     4021 atatatataa cgggaagata taaaacctaa aattatgata acctgctgca gcttctacat
     4081 agaaagagaa gcgctcaaca ccaacctcat ttatcaaacg ttggccataa ccccaaatct
     4141 cttctgatag ctgatcaaag gttatatact ttagctcata ttgctggata atgttcattc
     4201 taatcgactc catttattat cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaat
     4261 tttgtctttt ccaacgtaga tccccagctc atcactacct aagatcttgc caatgatatc
     4321 ttctggatag tatccttcag cgtttcgttc ttctttttga atagatactg atctattttt
     4381 tatgactgca gcgttcaata cttccgcaat ctcttcagtc gtcatttgtt cttttgctaa
     4441 attttgactg gttcgttgtt cattgtcggc atcaatttct tcagtatgat cagataaata
     4501 aaaccctgcc cactttaaac gtccacgatc attgtaatca atcattttta attcccccat
     4561 ttccgcccgc atgaccacca acaagtgtgc ttctagtaat gcttctagcg ccttctaaca
     4621 ttgaagaagc atgtacaatc gacgtaaaac catatttttg ccgaatcttg tcaataattt
     4681 tatctaaatt ttcttcgtca atttgctttt gtggatcact aaataaatcc agctgaagcg
     4741 aatcggtgta cacaagcttg ctataggtta ttccaacatg ccttacttct tgtccttcat
     4801 aatacttatc aaacaagaaa agacaataat tctgcaattc ctttgtatta tctgtgatag
     4861 ggatcttcat ctggtgtgaa aaccctggtc tcgtttctaa gatcgacgtg ccaatgtgta
     4921 aatggacaca acctgtttta cagttatgct gcctaatgcg tatcgctact tgttccgcca
     4981 tttcttttac caccagctcg atttcatttc ttctagtata atctcgtggt aacacttgtg
     5041 aatttccata tgatttttcc tttgtcggtt ctggtgggat tgctatatct gttcgatcga
     5101 tcccattagc atggaagtac agctgtaagc caatcacacc tagtctattc ttaatcgtat
     5161 aaggattcca gttagcaaga tctctaatgc tcataatccc catctgattg agtctttttt
     5221 tcattcgaga gccaattccc caaaagtccg tcatttcagg aatattccat actttttcag
     5281 gaacgttttc gtacgtccat tccgcaacaa atccttcatt atgctttgct tcgttgtcta
     5341 aggctagttt agccaaaagg ggattatctc cgacacctac tgtagcaatc aatcctagct
     5401 cttctttaat attctcctgg atcatttgag ccagcttctt tctccgttga cttcgtgttc
     5461 cttcagtcgt aaaaagattc agtgatcggg tcacttttaa gattgattca tcgatcgagt
     5521 aaatcagtag atcttcatca gccacatatc ttctgaaaat attatttacc tgcatatttc
     5581 gcttgatata taggttcata cgtggtggaa caacgtgtag tgttttagga aatggttgtg
     5641 gtaagtcacg tggtctactc acatttgtaa taccataccg cttttttgct tcaggagaag
     5701 aagctaatat caatcctgaa ccagtattat cagctcgact cataacaaca agttctgttg
     5761 ttaatggatc taaatttctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga
     5821 ttaaaaagta atcatttact ggctcttttg aataatccat cataaataac actccattct
     5881 ttcacattat acaaacatat gttcgatatt gtaaagaaga aatagctaaa tacataaaaa
     5941 aagaagcata ggtatgctat caccctgctt cttcttttga tttgtatgat caatttattc
     6001 tatcacttta ctaattaatt tgaaaatcta ataaaaactc tcactctaaa agagtgagag
     6061 actgcaaatt agagtacgga cttttttgga gttccgttct ttaatattaa caaatgaata
     6121 aaatccttac aacatttgag aataagttat cagtagacac ttacaaaaaa ttctcatact
     6181 atttcctcca tcattagttt ttgtctatat aaggtttacc ctaaatagac actggtataa
     6241 cattgaaaaa tatggcttcc tcgtgtatga ttagggtgta ttttaaatag ttgcagaaag
     6301 taggtaaaag ttttgagagt tgcatacata cgtgtctcat cgatcgatca aaatgaacaa
     6361 cgccagattg aagaaatgaa aaaattcggt gcagagagaa ttttcatcga gaaacaatct
     6421 ggcgcaacga ttactcatcg acctgttttc caagaagcat tagattacgt ccgagatcaa
     6481 gatattttta ttgtggaagc tattgatcga cttggaagaa attatgacga gatcatcgct
     6541 tcagtcaatt acttgaagaa gaaaaatgtg aagctgatca ttactagttt accgatcatg
     6601 gctgaagcaa ttggcaatcc tctcctggat aaattcatta aggatctgat catccaaatt
     6661 ttggccatga tcgctgaaca agaacggact gaatcgaaac gtcgccaggc acaaggaatt
     6721 aagatcgcaa aagcaaacgg cgtctacaaa gggcggccca aattatacag tgcagacgca
     6781 aaagatcccc aacgtcgcct tgtttacaaa agcatcgttg aagatcttaa aaatggtgtt
     6841 gcgattgcta agattgctaa agactacaat gttacgagac aaaccgtgta tagaataaaa
     6901 aaagacagca tggtcaatga caaatagttg atcatgctgc ttttattatc ttctcatctt
     6961 taatttccta catataatta ctatctataa aattaagtct tactcagaac agataaagca
     7021 taaaattcca acaaaccatt ttttacttaa ctaaataaag agtctgttaa tataatcttc
     7081 acttatttac gttaatcccc tactgtattt gttggttcct tatctatatt ttctacaaaa
     7141 tccttattat cttgtgattt agcttcgtat aattctttgt atgtcgacac attggttata
     7201 ctaattccta tagctagttc ttgagactct acttttctct ctagctcctt tgacaaagtg
     7261 atttgagatt tatttggttc cgttggagtc aattctatag attcttcata aaaatctcta
     7321 tacataaagc tatccctgaa taattcatag ctttaattct ttggcacatt ttattgaaat
     7381 atgtatcact aatatcccta tcaactgatt ttgactaaaa agttacttca aacattcact
     7441 gttcaaaaat attgaggaat aaattttggg tgtaaggcat tttgagcata cttctccctg
     7501 gataacttcc cagtaaaaaa atcgttagat tgttggatta gatccattga ctggctcgcc
     7561 tcaggcgagc aaagaccctg tagaattcat tctgatacac atgataacta gataatttga
     7621 gatagactcg tctacccgtt tggactaatt tcccagcaac tttcagaaac ttcaatcgaa
     7681 tagaatgaat ggtcattccc ttttggactt cttcaaagcc aatcgttctt aagaagttga
     7741 ctaagttgta agctatcaag ctgagcatca ttcggacatg attctccaaa aagcgaggac
     7801 tgtctgtttt gtcaaagtaa aagccagctt tcgcttcttt aatgaagttc tccattgtgc
     7861 cacgtttggc atagatagaa aagatggtat caggagaaac attttctgat agattcgtca
     7921 cgataaattc atgtcgaaag agtagttcgc ccgcttcacg tgttgagcgt atacacacgc
     7981 gacgactttg tgaccaggat tgtgcttgat aagtggtcga gaagtactga acttctcgtt
     8041 cttcccactt ttgattatcg ccataaagta ctgatttctc agctatttga cctagtctac
     8101 gattattctt cagtcgaata acataatgac tcttttttga ttcacacgaa tcatacacac
     8161 caggtgtagc gaacccgctg tctccacgaa ccaagatgtc agtgtttggt agagagtgat
     8221 tatagtgctc taataaaggt gtgagaaact cctttacgcc ttttgatgta tattgatttc
     8281 ctgaacgtag ttcagctttt aagaagtcac cagtcaatcc gtcaaaagct accaatggat
     8341 ggtaaccata ggtttggtag tgtgtattat aattcgtttg ttcttggtga ccaaatgtat
     8401 ctgaatgggt cgaatctaaa tcaatgatta attccgtatc attacggatg aggcgtgctt
     8461 tatcgataag ttcttggttc aaagcttgaa gttcatggat attctcctca gatagtcgat
     8521 ctaaaaatcg ggaaagtgaa gattgagaag cgagttcttt cctatctaaa acagctttaa
     8581 acacaggatc ttgtctcagg agattagctg cagaatcagc tgagtaacca gcaattaatt
     8641 gcataatgaa ctgctcaagt atggataagt tgtcatgagt aaaatacgct cgttcgtctt
     8701 caatgtgaat gtgttgctta gccagttcag aaaaaccaaa gatgttcatc agctctttca
     8761 ctaggacgag acccgaatca ctcgataatt gaccacctgt atgagaaata gtgatatttg
     8821 aattgaattt tacttggttt ttgtgtaagc tagtcatgaa aagaactcct ttcttttggt
     8881 tggtttagtc actttaacca tagcagaaag gggttctttt ttcatcactt aacgggtgaa
     8941 gatgaaaaag taattgtaag ctgccgtgag gcagtttcac atggttttaa ttaaaagtat
     9001 gaattattca ggctatcatt aattttataa aaaaaattaa atgtttttag cactgccata
     9061 taacattcaa aacattcttc tatagttggt ttacttcctt gaggagaatg aaccaattta
     9121 ttacgttttt ctatgaaatt tttaatttcc tttcttattt ctttcggaaa ctcaatatta
     9181 aagtaccctt taaacatgct ccctaataat ttgtctacag ggggagattg taaattctca
     9241 aatattagag ttaaatcagg tttctcttgt tgataaaatt cctttacccc tatttcacag
     9301 gctgtaatta tcgaaataaa cctatctcta gtatttgata tatttttact tcttttaagg
     9361 agtataagag aaaaggggat actttcgtgt ccatgcatgt aattaactaa ttgttgagct
     9421 tcagaagtta tatctctaat tatatagtta acggaattag gaagtgtctt taactctaat
     9481 gaaatttcta tccaattact atcttctaat ctgtatatgc tcgaagtata atttgattcg
     9541 tcagcaagca tcgctacatc ccaaacactg atcataaaat cttttaaacg ttcaatcatt
     9601 tcagcaatat tgctaaaatc agctaatttt tctttagaat aaaaaaaaac atttatatca
     9661 gaatcaataa attctatttt tttattacta tatcgtcatc attatcttga ccatttatgt
     9721 ttattgttcc ctgagtgggg agtacttctt tcaaattcaa gttaaattta tattccatta
     9781 cttctcctcc aatatataaa aattacttaa ctgtatccaa atttaatatg taaataaatc
     9841 attaattttt agctaaattc tatgagaagt ttatttttgt tgtttttgct aattaactaa
     9901 aactagtaca aaaaatttaa aatagattat taaacctatt gcttattcta ttcagtcata
     9961 tatataagcg tcatccttta gtaactaaca tcccacacta caatagtatt ataacatatc
    10021 tattcaactt caaattgttg aaatttaagc catttatatt tacttttcct caactttaga
    10081 aatgatttta ctaaccttat atccgtaata cgcgtaacaa acacattcat taataacaat
    10141 caacactaag ctatattagc tttttaaatg actagggtaa ataaaataag gatcaaaaac
    10201 ttttaagttt ctgacccttt ccttacttcc gattggttaa tagtgatctt ttgcagaata
    10261 aataaatatc gtttcatttt caactctata tatcagtcta tgttcatctg taattcttct
    10321 ggaccatttt ccagataaat catgctttaa tggctcaggt tttcctaatc cagcaaaggg
    10381 ggaacgatcg atatctttta ttaacttgtt aatctttttt atattgcttt tgtttccttg
    10441 ctcatgccaa taaagataat catcccaagc atcatcagac caagccttaa tcatcagatt
    10501 caacctcgat taagtcatgt gttttaaatg cacctttgga gaattgttca tctcctcgac
    10561 gaattttttc catgacgtaa ttattagaaa gtgttctcaa cgtttcttgc atagaatcat
    10621 aatctctttt tgataataca acaactgtat cttctacatc tttacttgtt acaataagtg
    10681 tttcagcatc ctcattaact tgtttcatat aactacgtaa attttggcgg aaatttgaat
    10741 aagctactgc ttccattcct ttcacccctt atttatttct ataaaacaat tgtacattat
    10801 attgtacaat taagcaaatg attaatattc taacttctta taattaaaag tgctatcact
    10861 tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc aaatcctgct
    10921 ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc acttttgccg
    10981 tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt aacgggtaac
    11041 cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa taaccctatg
    11101 aaaaatatgg ctgcaatcat cttgaatagt agactcacaa gggctaatac aaagcgccaa
    11161 aagatcaaac aaaataccag tattgccaaa aatactacga agtttaaatg cacagctgca
    11221 gcatggcgca taagtatttc gccaacaaat aggactaaca atagaggaat gattttattc
    11281 atatggatct ccttctttct cttaggttat cgggtgttgc ccgcttattt cgcaccacct
    11341 aagcggcggg ggcggttgga gcgattggtg cgaaataaga accattccct tagttactag
    11401 gttttttgtc cttatttcac acattctcac ttttcggtat aaatgattat tgcctttttt
    11461 tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg atttcttctg
    11521 tatcgtcgta agtataggtg tctaaaaaat cagttaaagt cgt
//
LOCUS       DAYYFD010000092        11441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000092 DAYYFD010000000
VERSION     DAYYFD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.092"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..2000)
                     /locus_tag="ACHA25_002345"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="ACHA25_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2439..3308)
                     /gene="scrK"
                     /locus_tag="ACHA25_002346"
     CDS             complement(2439..3308)
                     /gene="scrK"
                     /locus_tag="ACHA25_002346"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8763619.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(3328..4449)
                     /locus_tag="ACHA25_002347"
     CDS             complement(3328..4449)
                     /locus_tag="ACHA25_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8763620.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4451..4846)
                     /locus_tag="ACHA25_002348"
     CDS             complement(4451..4846)
                     /locus_tag="ACHA25_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8763621.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4865..5662)
                     /locus_tag="ACHA25_002349"
     CDS             complement(4865..5662)
                     /locus_tag="ACHA25_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8763622.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5664..6452)
                     /locus_tag="ACHA25_002350"
     CDS             complement(5664..6452)
                     /locus_tag="ACHA25_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8763623.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6466..6942)
                     /locus_tag="ACHA25_002351"
     CDS             complement(6466..6942)
                     /locus_tag="ACHA25_002351"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8763624.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(7014..9671)
                     /locus_tag="ACHA25_002352"
     CDS             complement(7014..9671)
                     /locus_tag="ACHA25_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8763625.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(9735..10094)
                     /locus_tag="ACHA25_002353"
                     /pseudo
     CDS             complement(9735..10094)
                     /locus_tag="ACHA25_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(10272..10592)
                     /locus_tag="ACHA25_002354"
                     /pseudo
     CDS             complement(10272..10592)
                     /locus_tag="ACHA25_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(10708..>11441)
                     /locus_tag="ACHA25_002355"
     CDS             complement(10708..>11441)
                     /locus_tag="ACHA25_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763626.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT         3666 a         2248 c         1625 g         3902 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 tgggtatcat gagtgaatga tattgctgaa tacaaatagt ccgaccacat taagttgatc
     2401 ggacttcata agcattttat tgactaatac ttttttgctt aagaattgat tctaacatct
     2461 tttgctaaag ttaagcatcc tatagttcct gcattatctg ctaacttagg tgtgacaatg
     2521 tattcttcga tatttggcgt atcgacatag ccgttaatta gttctaaaaa acgtctttgt
     2581 actttctttt tcagatgttc ttgttgcatg acaccaccac caaaaattat aatatctggt
     2641 gaaagcatca gggtcgtatt acaggcacat tgtgcaatat aatccgcctc gatttcccag
     2701 aacggatctt ctatagaaag atcttgtccc ttggttcccg tacgcatctc gatagcgggt
     2761 ccagatgcca acccttcgag acaatcatga tgaaaaggac aagtaccaga gaaagtatct
     2821 cctgaatgtc tcttaactaa cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa
     2881 atcccatctt gtatagctcc tccaccaatt ccagttccaa cagtgtaata aacaacactg
     2941 gataaacctt ttccatgacc tgaaacatac tccccgtaac aagcagcatt cacatcggtt
     3001 gtccaggcaa ttggaatttc gaaatgctta ttcaacgttc caacaaaatc aaaatttttc
     3061 caggcagttt ttggagtaga ggtgatatgt ccataggtct ttgattcacg atggatatca
     3121 atcggtccaa atgaccctac tccaatgctt tctaattgtt ctttatagtg attaaagaaa
     3181 tcaattacca aactcattgt ttcattcggt gtagttgtag gaaaactcac tctttctaaa
     3241 acctttaaat catcattgcc aatggcacaa acaaatttag tcccacctgc ttctattgag
     3301 ccatacattt cttactccac cgtcctttta tctaggaatt cgttcttccc tgtctaatag
     3361 tttttcgaaa aactcatgtg gttccttttg gtaccaatag gcaacagaag aaatatcgtc
     3421 ttgacgctca taaagcttga tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt
     3481 ttctttgaaa ctaatggggt cgggtaaatg ccagcggtac aacccatgca tcggtaaagc
     3541 atcatcgcca aaaccatgaa ctggatttgg attaggtttt ccagcactaa aataatcccg
     3601 ggtgtgatct cttttagatt gaaaaggata gcccataaat agcgtattaa aattttgagc
     3661 atatgccctt tctccaaagt tctttttatg gaaagcccaa gcgccgccaa aataatcttc
     3721 tgctcctgta gatgttattg tagggaaatc ttcatctcca tctatataaa acttaaattc
     3781 tccttccccc caccagtatc tttccaatgc gcacaaagca aagtaggttc ctacataata
     3841 cccagtgcct ttgattccat caataagggt gtaatctttt ccttttgtcg tgatgggttc
     3901 acggttccat ttagcatgaa aatacatcgc atcatccggt acttcagtca atgcataatt
     3961 aaatgtataa aaaatgtgct taataaactc aggatgttca ttcgttaaag taatcttagc
     4021 tttttttcga aatggcatct caaaatagca attcatccct gatgttggat tcactacgat
     4081 tggtaaggaa ttcacttcgc atcttgtccc aaacccatta caaaaaaaat ctcctaatgg
     4141 actttctaca gaaggagtgg attcatcatc ccaataaata cgcaaaatta aatcacgtaa
     4201 gacgaaactc ccagcgattg ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc
     4261 agggccttcc atttctgcta aagtaattgt ctctccagca tttatcggaa cagaaggtga
     4321 cccttttctc ccaattccta gatgactcgc tgtcattgcc gctttccctt tttcaccatt
     4381 tttattttct ggtgaaatag ttctactttc ctctcccttg tggagccata tattatttaa
     4441 acaattcatt ttattcgtcc tctatccttt cttcactttt ttgattattc atatagatga
     4501 tttgttgttg tgcctccgca ataattgctc caatatctaa attatcattt cctaactgca
     4561 aagcgattga cattgccaag ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa
     4621 tataggtaaa aaacttttga tttacacttc ctccagccat gtcagttaaa acaattattt
     4681 cgtctgtagt atccactaac tctttaattt gttcatctat atcatcaatt tcttcgacat
     4741 atgcacataa gctactaatt tgattatgca tacctgttaa aaactctagt gtctctttaa
     4801 aaccctcagc tagtattgaa tggctagcaa tgatcaatct tctcataatt caaatccttt
     4861 tcctttacgc taatattcca aatgcagcca taaccatcgc aaaaacaata acgagtagaa
     4921 tcaaaatggt catactgatt tttttcttac tgagtagata atatgctgca gcagtaataa
     4981 ttactggaat taatgttggt aaaattttgt ccaacattgg ttggattttc atactaactt
     5041 caccaaaagt gaatttaagt ggcgttgaaa cactgataac tgaagcaatt aatgatccaa
     5101 ctactgttag ccccaatact gaagcagctt cagttaaata attaatcttt tcaccgtatt
     5161 cagtaacaat cttcattccc tgcttataac caaattcaaa gcctctcatt cggaaaaaga
     5221 agattaccat gattaaaatg aaccataaaa agattcctac aggattattt tctaaagcca
     5281 tataagccgc aattgatccc atgatggttg ggatcatcgt catcaaaaga gagtccccaa
     5341 taccagcaat tggacccatc gtaccaactt taaagtcatt aatcgcatct tttgcttcta
     5401 atccttgctt ttcctccatt gcaacacaag cacctaaaat aatggctgcc agccaaggct
     5461 gtgtattgta atactgaaag tgattattta aagcttcgat gtattcatca tcatctttgt
     5521 ataattttct taaaataggt gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat
     5581 aattaaaggt acaaactgac atcaaccaac gatagttcgc tttcctaaga tcctttttcg
     5641 ttacttgata tttttcgctc attttattca tcctccccaa taaacccaac atcagaagct
     5701 cccgcagaag cagtgatttg tgcatctttt tcagctctat gatacacatt aaatgctaaa
     5761 gcgaatccta tgatcgccgc tccaataata ggtaccttca aaaaagcggc taagaaaaat
     5821 ccaacaagta aataactaat gtatcgacct gtaggcataa agcgtaacaa catcgcaata
     5881 ccaacagcag gcaacatttt tccggctaat gttagcccat ccataaacca attaggtaaa
     5941 acatccaaaa cgtaattaac agcagactgt cctaaagtaa cggcaataaa tactggaatt
     6001 gctgcagata aaccaaagaa aattggcccc aaccacaaaa cactattcat tttcttaaat
     6061 tctcgtttat tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa gattttgaca
     6121 attacatcta gttgcacccc taacatacct acaggtagtc ctattgctaa tccggcttct
     6181 actccaagct ggctaccaaa tgccgttgca ataattgcgc acgttccata gtccggtaca
     6241 gatgagccac caaaaccggc aactcctaat gacatcaatt gaactgtacc gccaactatc
     6301 aaacctgttt ttatatcacc catcaaaatt cctgtgaata atccccaaaa aactgcgtaa
     6361 tttacaaaga tcatcggaat cccataataa tcaacatgtt taataaatgc taatatagtc
     6421 agcataataa tttgaaaaat agtaaaatgc attatcctta ctcctttatc ctaattttga
     6481 ttgaatttcc tgtgccttat ccgccggaac aaattgtgta acaatttcta caccagaatc
     6541 caacaatttt ttatacgtgc tgatattttc atctgatgca taggctctag aagatagctg
     6601 taatgcatta tctttaacaa cagtaccgcc aacaattagt cggtcaattc taataccgtt
     6661 atctactaaa tgaaggattg tttccggctc tttcacgatg acaaatactt tttcctgatc
     6721 gtatttcccc tttttaaaat tcgcaatcgc tgtttcttca gtgataattg atacagccat
     6781 tccagcaggt ctagctaaac gcatacttga tttttggatg tcgttatttg cgacagaatc
     6841 atcgatcacc atcactctgt ttgctttaga cattggtgcc cattgcgtga caataatccc
     6901 atgcaataaa cggttatcaa ctctcgacat aacaatactc ataaatactt cctcctttat
     6961 ctttcatttt cttacaagca attaccatgc caaaacaaaa agaacttgat aaatcaagct
     7021 ctttttacat aatcataaac gtatttaacc tcatgaatcg ggatgtcgac actataataa
     7081 tgttcaacga cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc
     7141 gtttcattga aataaatttc atcttccaaa gtattttgtt ttactaatcg ttcaattaag
     7201 caactaatgt gaacatataa tccgaaacac gtattattat caaatgaaat tcctagatca
     7261 ttttgtaaga tgtatatagc atctgaaacc cgttctaata atttagtcgg atttaatatg
     7321 gtcaattgct ccattaaatt cgttagacta aaattcttca gaatattatt gttcaaaact
     7381 ttcagttcat tatcattaaa gtgtctattc aattgattgg agaattttct tatattcatt
     7441 cctaaaatca tatcttcaat tgaaaagaat tctacatctt ctagatgtgg atttagtgtt
     7501 ccaacaataa aaagaatatt gttctcttta cgaagctccg ataaatagtt ttcattcact
     7561 aaagagtaat agtcacacgt ttctatgaca agatcagtat cccttggaaa gctatcttca
     7621 agtatccttt ttaatttaaa cgaagttcct aatccgcttg cacaagaaca gacaatcata
     7681 tccttcttaa tatttgatga ttcataaata taatccacat tttgattttc ttttacttgt
     7741 tcaagaatct gtttaagcgg taaatctaat aatatctgat ttgcaacatc aatagccatt
     7801 cttgttgaaa cattgtttag tagtccgaat tttttttgag cgtctagttc caaatttaga
     7861 taaattttct ccagtgaacc catatcaaca agtaaaataa tttcttcata tccggtacac
     7921 tcttgtaagt atctattaat tttgtctgca atctcactac ttgaagtcat tagtggcata
     7981 tcaatggcgt cgtatatata tctctcaacg atcctattag cagtacttgc tatacaggtt
     8041 gccgttagat ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa ttctttacta
     8101 cacattaact taaataaaat aaccaaaaca tttttttgaa tttttgttaa ataaatatcc
     8161 cctatttgaa taaaacgatt gactaattga acttctcgac tacaccaact attaatatca
     8221 ttgatgaagg aatataattt atcgtccgat gttaattgga tttgataaat ccaagaattc
     8281 aaaaagatag acgttatttg aagataataa tagtcaaatt ctatttgaaa atctctctct
     8341 atttccttaa catactgtct aaatcgaatc tcttgatagg tgggatatgt taaagctgaa
     8401 ttattttgga tatcaacatt cttttgtcta aaatatctat catatttctc aaaaagctgc
     8461 tgcttatcag tgtctttctt ccacgtttga taaagatact ctataagttc gttgatatta
     8521 aaaacttcgt ctactttttc caaatcatcc atttgaatat attttaattc attctgtctt
     8581 ttcataacct tcatgccgtt gaaattcacc cattgtggta aatgatacat ctgaatataa
     8641 agttcttcca aatcatctaa gaaataaaag gcattggcac aggtcaattg aatgatattt
     8701 ttcaaattgc caacattttc actaaagcga tagtttaata aaaaatctaa taattgttga
     8761 gatatcagta ttttctttgc tagtctttct tcttctgatt taaaaaatat cttgattaac
     8821 tgttttcttt cataaagaga acgactgtct aatgttggaa gtgttagctt tacaggtatt
     8881 cttctcaaca aagttgataa tagtttatcc tcaggtggtt ctgatgttgc aaatataatc
     8941 cagcaacttg atgtgtacca gttttcatta tcacccatcc gatgaaattt cctcgtatcc
     9001 ataaactgaa aaagtttctc ctgacattca ttgctcaatg catgaatttc atctaaaaat
     9061 aagactccat tattagctaa ttctgctaaa ccctttcttc catcttcggc tcctgtatat
     9121 gcacctttta catgcccaaa aaaattggac aagaaaagtt ctggattatt ggcatattca
     9181 ctacaattaa ctgaaatgaa agatgccttc tcttctatat aattattggc caacaaatcc
     9241 ttatacacat aatttgctaa atagcttttt ccagttccag tctcgccatg aagtaaaatg
     9301 ggtaagccat ttggcggata gtagattgct gctttaattt gagcaattac atttcgcaga
     9361 cttccttgac ttccgataac cctttctatt ccaaccctaa aagagctatc ataccatttt
     9421 tccgcttcct ctgaagagct aaaaatttgt tgatttggaa ctttctccgg taaagaaaag
     9481 aagattactg gacgagtatt gatttttcca aattctccat cttttacgaa ttcattcaaa
     9541 tattgagaga caatattacg actaacattt aatttttcag atattttctg tgctgtattg
     9601 ttcccccatt tatttttaat ctcagagttc tctatgacat aattgacaat gcgttctttt
     9661 aattcttcca tcacagcacc tcctcgacac tattcgaatt ttgccgacag tatttttcca
     9721 taaatatgac ttgctaaaaa ggctttgttt agatcattca attggaacgt tgcttgtttt
     9781 gcttgatccg ctaagacgtt gaaaagatac tctggcgaaa tcgcataccc caaaatatca
     9841 acgattgttg caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac
     9901 atcgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact
     9961 tgttccaaca aacggtcgga taaatattta tagaaaatca gccccaataa ataattttta
    10021 tattctgacg catccatttt actacggaga ttatctgccg cactaaataa cttctgattt
    10081 aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt
    10141 attttagcat actcagtttt agaaaaagca aaaaaataaa gaaacttatt cttgataaca
    10201 agctacgtga cgtatatccc taacacaaaa atagccatat cctttggttt ggatacggct
    10261 atttgatatt actcattcaa ttatcaaatc acagtaggtg tgaatctcat cgggatcggc
    10321 aatatctact tctgattagg atcaaaggtg attttctcaa taaaggtgtg atacaaagct
    10381 ttaacttgct tcttctcact ctggataaga aagcaatcca aactagtcaa tatctgttgg
    10441 acatctttga cttgaatcgg ttccccttgt tgttgaaaga tccctaagat ttcattctct
    10501 cgaatttgta actgtcttcg tttttcagtc aattcattta aacgatcttt taacatcgga
    10561 aacaattcgg gactatcttc caaagcagct tcctgttttt gctctaatag ttagattttg
    10621 cgtaccattt tcttctttta ttttccttcg attgctgatt ttaatatagt ctaaaggaaa
    10681 tttatattta ttgtattctt tataacttca agagaacttc gtttgagtat tactgtattt
    10741 ttttaattcc ataagaaaat tagttattac ctgccaccta atcagcaaat ctgaacctga
    10801 gtcaatataa ttaatattat tttccatctc caacattgta agtactgttg gtgttaatat
    10861 agggactttg tacaattgta tttttttgat tttatcatct gcacaaagga gattttttaa
    10921 atctaatcct tcttttattt caaaagttga attttttaaa tctataagag taaaaccaat
    10981 tccttcatac tgaatttctg aaaatcgaat ttttctccca gttgatagat caaggggcag
    11041 aaaacctttt attacattgt gagtgtcacc atcatttaca atgtcattag gaattttgat
    11101 tttagaattc tgtggagcac ctattggaag ataataattc attataattt tgtagtcttc
    11161 atgaggaaat aaagtacttt taggattgct aagatattct cgtaccgaat ctaagaatgg
    11221 cttatccatt gaatcttcat ccccatttaa actaaaaaac attcctacaa ttgcttttgc
    11281 aactctaagt gaattcattt tctttattga atgtgttaaa tcagacttac cattctttaa
    11341 acttgtattg ttataaagga aatttttatt gtcttctgca aatctaatat attcctcaac
    11401 atactcacta ccaaaaaaac tattacattt actacataac g
//
LOCUS       DAYYFD010000093        11222 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000093 DAYYFD010000000
VERSION     DAYYFD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.093"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..511
                     /locus_tag="ACHA25_002356"
     CDS             <1..511
                     /locus_tag="ACHA25_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763627.1"
                     /translation="EIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCS
                     DSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRS
                     NRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDED
                     NPKTYDILA"
     gene            complement(572..937)
                     /locus_tag="ACHA25_002357"
     CDS             complement(572..937)
                     /locus_tag="ACHA25_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8763628.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1013..1258)
                     /locus_tag="ACHA25_002358"
     CDS             complement(1013..1258)
                     /locus_tag="ACHA25_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8763629.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1328..1582)
                     /locus_tag="ACHA25_002359"
     CDS             complement(1328..1582)
                     /locus_tag="ACHA25_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763630.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1746..2069)
                     /locus_tag="ACHA25_002360"
     CDS             complement(1746..2069)
                     /locus_tag="ACHA25_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763631.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2256..2329
                     /locus_tag="ACHA25_002361"
     tRNA            2256..2329
                     /locus_tag="ACHA25_002361"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2290..2292,aa:Ile,seq:cat)
     gene            complement(2714..3211)
                     /locus_tag="ACHA25_002362"
     CDS             complement(2714..3211)
                     /locus_tag="ACHA25_002362"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8763632.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3251..4060)
                     /locus_tag="ACHA25_002363"
     CDS             complement(3251..4060)
                     /locus_tag="ACHA25_002363"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8763633.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4394..5251
                     /locus_tag="ACHA25_002364"
     CDS             4394..5251
                     /locus_tag="ACHA25_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8763634.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5244..6146
                     /locus_tag="ACHA25_002365"
     CDS             5244..6146
                     /locus_tag="ACHA25_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8763635.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6179..6529
                     /locus_tag="ACHA25_002366"
     CDS             6179..6529
                     /locus_tag="ACHA25_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8763636.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6578..7501
                     /locus_tag="ACHA25_002367"
     CDS             6578..7501
                     /locus_tag="ACHA25_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8763637.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7579..8472
                     /locus_tag="ACHA25_002368"
     CDS             7579..8472
                     /locus_tag="ACHA25_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8763638.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8510..9073
                     /gene="amaP"
                     /locus_tag="ACHA25_002369"
     CDS             8510..9073
                     /gene="amaP"
                     /locus_tag="ACHA25_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8763639.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9087..9275
                     /locus_tag="ACHA25_002370"
     CDS             9087..9275
                     /locus_tag="ACHA25_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8763640.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9288..10190
                     /locus_tag="ACHA25_002371"
     CDS             9288..10190
                     /locus_tag="ACHA25_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8763641.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10222..10464
                     /locus_tag="ACHA25_002372"
     CDS             10222..10464
                     /locus_tag="ACHA25_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8763642.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10720..11091)
                     /locus_tag="ACHA25_002373"
     CDS             complement(10720..11091)
                     /locus_tag="ACHA25_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763643.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3972 a         1924 c         2130 g         3196 t
ORIGIN      
        1 agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc gttattattt tagtctattt
       61 tcaggggggg aaccttatga taaaaaaact atttttacta tcaatattaa cgctagcaat
      121 ggcaggttgt tctgactctg gcttagcaac tgaactgatc acaaaatcaa ctgaagccac
      181 gacagaggag ttaaaatgtg gaaacgatca cagtgactac agaggcttaa atcgacgatg
      241 catgggaata ttaggcagaa gcaattgtaa gcatcgatgt agtaatcgaa gaaggtgtag
      301 aagaagcaac agaaaaatca aaagaaagat caaaagaagc aatctgtgct gtgcatcgta
      361 tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg agatgttcat ttatgtctag
      421 ggaagttggc gatgaaccaa aactagagta cgcttttagt tttgcacgtg aggatgaaga
      481 taaccctaaa acttacgaca ttttagcttg atctaagatg gttaaagctt tttaatgaag
      541 taagaggaga tagaaggctt ttctagttat attaggtaag ataaattcct ttttgctcca
      601 aatttgctaa acattctgct agataaactt catcgtgttc cggataaatc ggcacgttgc
      661 gctctacagt aggtaaatca aaaaatgctt cgcatgattt gccacgatac ataggatttt
      721 tccactctgg tgctgggctg taaccacgtt tttccatttc atccatgaca agctgatgat
      781 attggaataa tctataagga gaatagtgaa aaacataatc cacagtagca tgtttctttc
      841 cccagccatt tcctcttaat gcacagcatt cccgatgttg tcctaaaagc tgttgacgag
      901 gaagctttga aatcaatttt tcatgccata atcgcataga aatgtctcct ttatatatag
      961 attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa ggctggaaaa aatcacttca
     1021 gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac ggaaatatcg taaaagtcag
     1081 cgatatagat taattcaatg atgcttggaa tttcttctcc ttcttcgtac ttacgatatt
     1141 tgaataaagg aatacttaac gcgctagaaa cagctacttg agataaagat cggccagttc
     1201 ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat agcttcttcg atactcattt
     1261 aatcaccttt cattatagta gaatagttat aatgactata gaaagctaca catgcaatca
     1321 atagtactca atgtttttta tttaaacgta tcaaataaag aatgacgatc aataaagaca
     1381 aaaccagtcc tgataattct tctataaaaa cactatcggg ttcaaagtag ttgtaattaa
     1441 aaactttcca cataaagtag atagccggaa gagtcaaaat agctaaataa aaccgttctt
     1501 tgttccaatt cataagataa acaatacttg cgaccagtgc gaccgcaggc acccaaaaca
     1561 aaactctcac caacgtaatc atgtaacgga ctcctcaacg attcatataa taaaagataa
     1621 catgttattg catacatcac aaatgaaatg ctaggaaaat aaaagaagaa tagatcaatg
     1681 aaaaatgaaa aagcagaaca gttttctgct gataagcagg aactgttccg ctgaatgtgt
     1741 cttatctatc gagaaaaaat caatggattg atatctaagt aattcaatac atataaaaag
     1801 aggacgatcg cattcactaa aaaggaaaga atgatgatcc catagcgaat ggaaacatat
     1861 ttaacaaata cttttgtttg aatacgggca tatctttttc ctttatagct gagcatcatg
     1921 acaagaagca aaagggtaat cacaccttga aaaatcattc caataaacag aattcctgag
     1981 gtttgataat ttaattggag aaaataagct gctgccgcaa ctaaatcgaa taaaatcgca
     2041 ataaatggca aaataatcac tcctaacatt gagaataaat aaaagaataa cattttttct
     2101 aaaataagca aataaaaatt aaagtaacaa aatagttatg acacaagtaa aagaaataag
     2161 aataacaata ttattttact tatattttga aacttgtgag agtttgtcat tcgtgctata
     2221 gttgaaaagt aacaaaaata aattttattt atgatgtacc tttagctcag ttggttagag
     2281 cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta caaggtacat aagaggttgt
     2341 gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt
     2401 ttacttggct gttcttatta ctgcagatgc aagcttttga ataccgttta aggaagaggt
     2461 tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa cggaaaaata gttcctcata
     2521 ttttgccgtt tgttaagcta ataccgaggg tcagctgatt gaacaccatt taagaaagag
     2581 gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc aatctacttt gatagaaaaa
     2641 gcacgtagga ctaaaacagt tctgcgtgct ttttttattg tgtgttaaat atcgtaaatg
     2701 aagatagata tttttatgat tcgacgatag agacgttgaa atctttcaac agctctaaag
     2761 cggattctcc gtgttttcta ctggagacta acattggatc aacaaaaaag tctgcttcag
     2821 gaaaagcact ctgcaggcct atcagattag agagtacaca caagtttgtt tcaatcccga
     2881 ctacttcgat ctctttttgt tcttctttgc tttcaaataa agtttcctgg atttcagcta
     2941 aagtttcagg tggaatcgta aagtaatact ttttaatcac tcgttcattt tcttgtggag
     3001 aaagaagagg gatcaattgc aaatcttctc gttcttcttc tttgtccttc agttcaactg
     3061 gtatatcccg tgtgaagaga atatattcat tattttggcg tgctttagct aatctttgag
     3121 caatacgttc tgccaattga tctgactctg gaatataaaa ttcgctgttt ggatcaagaa
     3181 tatgattttg catatcaata ataactaaca ttttctttcc tcctacattc atatcgtcat
     3241 tttattcttt ttaatagaag ttttttaaaa tataagcaac accagcttct tcattcgtca
     3301 gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc attccccatc gcaatactca
     3361 acccgctgta attcaacata gcaatgtcgt tttcttgatc gccaaaagcc atgatgtcac
     3421 tcgattcgat tccaaggtaa tccgccagaa aaacaagtga atgagcttta gtgatacctg
     3481 taggcgtgat ttctaagaaa tgcggatcag agaatactga ttggatacca tctgcttgca
     3541 gatctctttg tagcttctgt agtttttctt cactcttatc tgttaatgct aatttaagat
     3601 agctaagaga aggatcttcc aacatattta aaatttgatc aaaagaatgg ttctgtaaat
     3661 tgaaatgttc ataataaggg tcatctttga ctagatttga ttcatcgaat gtcagctgat
     3721 catcttgggt ttctcggcta gcaatgatgg atattttcgc ttcttgagca tagaagaagg
     3781 cttttctcag tgtatcgcta tctatttttg aacgtctgag aactttcgcc atttttccat
     3841 ccttcatttc agcaatcagt gaaccattgg aagaaatcaa atagccaccg attcctaatt
     3901 cggaaaaaat atctttagca gaaatgctgt tccttccggt agcgacggtt acgattccgc
     3961 cattttcatc aaatttccga agtgcttgga tattttcttc tggtatcttt ccatttcgat
     4021 caagcaaggt accgtctaaa tcaatcgctg ctagtttcat tttatcttcc tactttcttt
     4081 ttacatatct attttcctgt taccttaaga ttaaatgaga atttaaaaaa atgaaactaa
     4141 cttgctttgt cattatgaat tttttcaaaa aacattctta agaaaaaggt ggctatatat
     4201 agtagttttt ggtcaatacc tttgaataaa aagccatgaa aaacgaatag ttagcgctta
     4261 ccgatgcgtt ttgtgataaa aaaattttaa aatattcaat gaaaacaaag catatatttt
     4321 gctttaaaac caaatgagga atattctaga tgtagaacag gttagaaaaa gattagaaaa
     4381 aggagtgatt ttgatggaga agagatacgg cgtagttcct gtggtaactg cttcagacga
     4441 aaattatgct ccttacttga gcgtgatgat cgcaactgca ttagaaaatt gcaataaaac
     4501 aagacgcata aaattttatg tgattgacga tggtctatca gaatatagta aagaaggatt
     4561 ggaagaaacg gtaaataaat attcaagtaa tgcaagtatc caattcctga cagtggaaaa
     4621 agatatctac gaagacttcc tagtcagtga ccatatcaca actaccgctt acttaagaat
     4681 ttctttacca aacttattag ctaaagagga ttataagaaa gtcctttact tagattcaga
     4741 tgtgttagta ctagacgata tcgtaaaact atatgatgag ccgttaaatg gaaaaacaat
     4801 cggcgctatt atcgatccag gccaagtgaa agctttggaa agattaggaa tcgattccga
     4861 tgacttgtac ttcaattcag gagtgatggt gattgacatt gatcaatgga ataaaaaaga
     4921 aattacagaa aaaactatcc attacctcag tgaaaatggc gatcgaatca tctatcacga
     4981 ccaagatgct ttgaatgctg tactttatga agattgggaa caacttcatc caaaatggaa
     5041 catgcagact tcgctgattt ttgaacgtca tccagcacca aatgaaaaat atgaacgtca
     5101 gtataaagaa ggaaatgaaa aaccttcgat cgttcacttt acaggacatg ataaaccttg
     5161 gaatacgttg aaagatcatc cgtataccaa tttatactta aaaaaattag cacacagtac
     5221 tctaacgaaa gtaggcgaga tcaatgaata aaaaagaaat agcagtagtc gcaagttgta
     5281 acacaaaatt cgtaccacat ttagctgcat tatttgtctc agtattagat aattgcaacc
     5341 cgtctaaatt tgttcggttc tatgtgattg acgatgatat cgattttgaa agtaaacagc
     5401 ttttacgctt ctcagtgaaa aacgcacgaa tgaactcaga tgtggaattc ttgaaaatca
     5461 ataaagaatt ctttacaaat gtggtaatca gcgatcgtat tccagaaaca gcttattatc
     5521 gtattgcgat tccggaattg ttccgcggca cagaagttga acgtatctta tatatggatt
     5581 gcgacatgat cgctttacaa gatatctcta aattatggcg tcttgacttc ggtgattcaa
     5641 tcgtagcagc tgttgaagac gctgggttcc atcaacgact ggaaaaaatg aaaatttcag
     5701 ctaaatcaat gcgttatttc aactcaggtt tgatgctgat caatgtgaaa aaatggttag
     5761 atgaaaatat tacgcaaaaa gtattagatt tcatcgaaca taatccagaa aaattacgat
     5821 tccatgacca agatgcatta aatgctatct tacatgaccg ttggttacca cttcatccgc
     5881 gttggaatgc acaaggctat atcatggcaa aagcaaaaaa acatccaaca gcagcaggtg
     5941 aacgtgaata cgaagaaact cgcaacaatc cttacattat ccatttttca ggacatgtga
     6001 aaccgtggag caaggatttt gaagggccaa cgaagaaata ttacgaaaaa tatgcaggga
     6061 tgacggcttt ccgttgtgta gctaaattcc ctaaatatcc aaaatatgca aaagttcaac
     6121 caaaaacaga tgtagttgtt aaataataga agaccacaac tgaagagggg gagtaggaat
     6181 ggaaattaca atgtctaaaa atgcagtaga gacgttaatt gaaaaggttg gggaaaatac
     6241 aaaaatcgca ctcgcgctta tcaatgattc cgatccattt ctaagggaca aaggtgcttt
     6301 tgcaaaaggc agcttttttc aaatcattcc ttttgtttcg gaatttggag aatatgcgac
     6361 taaaatcgag catccattat tggacatcta tacatctaaa ttagagcaaa attatttcgg
     6421 taaacgattg aacatggact tcaacaaaca gcttgacagc ttttcattag aaaatgaaat
     6481 cgcagtactc gattataata tcaaattaaa gaactgcttc ttcagctaat agtttcgaga
     6541 aagtccaaaa aggaggtata cattgagata ctactttgtg gatgaagaaa agaatgtaat
     6601 agaaacagat gaaaaaaaat ataattggct cgtacttgat agtgcaaagc ccgaagagat
     6661 tgagcaagca atcgaagaat tcgagcttcc tgatgatatt ttcgtaggaa ccgaatatcc
     6721 ggaagaagtt tctcggatgg agcgccttta tgagacgaaa ctagagcatc cattttcttt
     6781 ggtagtgatt aatctagatc agtcagataa tcgtatcgaa aaaaaattaa cgccaatatc
     6841 ttttgtactt tccgataatc tcttgattac ttgtatcgat agaaaaaccg aatttgtgga
     6901 tcggctgctg acaaagcatg gaaatcattt aaactcgttt gaaaagatca tcacgtatac
     6961 attattagat atttacaccc attacgtgaa agaactaaga gagatgaaaa agcgaattga
     7021 cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa gaactgtata aacaggcaga
     7081 tcttgaaaga gacatcgtat atatcgatca taccttaaga gatcaacggg aaacaatgga
     7141 tcaattatgg aattctgaaa ctttcatcaa acgtttagac gatgaacagc tactctatga
     7201 tgtccatttg agacagcgac aaacagaaaa aatgatcgag atctatcgag atttattaga
     7261 gagtattggt gatctattca acgggatgat ggacaataat ctaaatcata tcatgaaata
     7321 tctagattca gctgcattaa tcatttccgt tccggcgttg ttcgcaggga tttggggaat
     7381 gaatacagga ggattaccgg gtaaaagttc tgctctaggc tttatactcg tagtctttgg
     7441 ctctttgata gcagcagtag ttttaggtta ccatttatac cggaaagatt atacaaatta
     7501 aagaatctat ttaaaacagg tatcaaacaa agaaatttgt tttggcaata caatttaaaa
     7561 actaggagga atacagcaat gacagaacca aaattaatcg acccgcgaaa cttgtaccat
     7621 acggagaaat ttcctaaaca agatcaggat acaccagctt tacagcagaa tatgattcct
     7681 gttcctgatt gtggagaaga atcctatgaa ggcaataatc aactagaaaa ccgccgtgta
     7741 ctaatcactg gtggagattc agggatcggt cgtgcagcag cgattgcatt tgctagagaa
     7801 ggggcagata tcgctcttca attcttccca ggagaagaaa aagatgcaga ggaagtagca
     7861 aaatacgtga aagatgcagg acgtaagatt gtcttactac cttttgatct tcgtgacgaa
     7921 caagcaccta aagaaatcat agataaagcc gtgagtgaac ttggcgggct agatacgatg
     7981 gtactaaacg caggacagca aatttcttgt cagtctattg ctgagttacc acttgagcaa
     8041 gtcaaagaca catttatgat aaacatcatc gctatgtttg ctttagtgaa agaagctgtt
     8101 ccgcatatcc cagcaggagg agcaatcgta acaacaactt ctgttcaagc attcaatcca
     8161 agcgaacatt tattagatta cgcagcaaca aaagcttcaa tcgctaactt tacgatcggt
     8221 ttagcaaaac agcttgcacc taaaggaatc cgtgtaaatg gtgtagcacc tggtccaatt
     8281 tggacaccat tacaactaga cgaaggacaa ccttctgatt cgatccctga atttggtcag
     8341 caaagtttac tagaacgtgc aggtcagcca ggagaattag caccagttta tgttttctta
     8401 gcatcgaata aagcaagtta tgttacagcc caaatttacg gagtaactgg tggagaagcg
     8461 atcaacttat aagaagttga tcgtttcacc cctatgagga ggtgcgaaaa tgaataaagg
     8521 tgtaaaaacg atcggagtga tcggaacatt acttttgcta tctgtgcttt tgtttgcgat
     8581 actattaagt gcaacctatc cattgccttt tcagctagag aagtttcgat tcttttccat
     8641 cactaattca tacgtgcaac aatatgtatt ttggcttgcc gttgcatttg ctattttgac
     8701 aattatcacg ttacttgttt tcctgtttta tccaaaaaca atcggaaact ttctactgaa
     8761 acatggtgat ggggagttga cgttggataa aaaagcaatt gaaggaatgg ttcgttctca
     8821 cttgcatgaa gatgaattca tccattcacc aaaagtaaat gtcaaagcaa cgaaaaacaa
     8881 gatcgccgtc aatgtaaaag gggagttgaa acgaacttca gcactcattg gtaaaacagg
     8941 aacattgatg aaagaaatcg aaaatgaagt acaacacata ttgggaacaa aagagccagt
     9001 gaaagtatcg gtgaaataca caggctttga agaaccagct aaacgaagca atgaccgcaa
     9061 acgagtagaa taggggggga ccaaaaatga aagaactatt ttcgacatac aagctcccta
     9121 tcatctttgg ggtacttgga ttgatattag ccgttttatg gatcaccgtg ggctttttca
     9181 agaccttatt gatcttgatc atggcagcga tcggcgtcgc tatcggtttt tatctgaaac
     9241 aaacgaaaat cttagatcac tattttaaat cttaggagga aacatccatg aataacaata
     9301 acaaaaataa taacaataaa atgaacaacc aaaaaccagt gtctgcacat ccagacccaa
     9361 cgacaccaaa ccaatcaaat ggtgtgaacg atgaaaagaa caaaacagca ccagtatctg
     9421 cacatccaga tccagtagtc cctaacgttc aacaaggagt agaagagaag aaacatgatg
     9481 cagaaaagca tgcacctcat catccaggaa ctccagaatc taaagttcca gaacaacatg
     9541 atgcaatcga tccttctaaa cctgtggaag agcaaaaaca tccttctgac catgcgaaag
     9601 cacaagaaaa acttagttct gaccacacaa acggacaagg aaaacttggt tctgatcata
     9661 caaataacca aggaaagcat gattctgaac aggaagattc aatgcgcggt gagctgaatt
     9721 atgaagataa agttgtgcaa aaaatcatcg gaatcgcaat tgaacaagta gacggtttat
     9781 tatctgcaaa cggtggtttc ttctctaatg tggcaggaaa acttgtgaat acagataatg
     9841 taacggctgg gatcgaaaca gaagtcggta aaaaacaagt agcagttgat atggatgtca
     9901 ttgtggaata tggcaaagat atcgaaaaaa tctttgaaga aatgcaagaa gtaatcggca
     9961 aagaagtaaa aaatatgaca catttagaag tgatcgaagt caacgctaac gtagtggaca
    10021 tcaaaacaaa agaagaattt gaaaaagatc aagaaacggt gcaagacaaa gtaactgacg
    10081 cagcgaaaaa aaccggtgaa tttgcttcga atcaaacaga taaagtaaaa tcagcagctg
    10141 gaagcggcgc acaaaaagta aaagaaaata cagaaccacg ggttcagtaa taaggaagta
    10201 aaacattagg aggaacgaaa catgggattt atttggtcac ttatcgtcgg aggaattatc
    10261 ggagcaattg caggagcaat cacgaataaa ggttcttcga tggggattat tgcaaatgtc
    10321 gtagcaggtt tagttggatc agcacttggt caagcattat taggttcttg gggaccaagt
    10381 attgcaggaa tggcaatcgt gccatcattg attggtgcca tcattttagt actaatcgta
    10441 tcattctttg ctcgaaaact gtaaaaagac ttttaaaaag aggtcgtgaa aaagctgttc
    10501 ttcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc atttggttgg
    10561 gcatattatc gaagatgcaa gcttttgaac actgtttata cggaataaga ggctgtgaca
    10621 agacttttgt cacagcctct tatttatgcg ggcaaattcc caattagatt tttaaaagtt
    10681 tttccttaaa tgtgtcttta ttgatacaga gaagcttctt taataaaaca atgcctccct
    10741 aaattttctt gagataggaa aggtgaacat acaaaataat acgaccactt tgaaaaccca
    10801 gatgatccac aaatggatcg tatgatactg atgaagtaaa aagcaatgga tcagaaactc
    10861 aaagagattg aaaaagacaa ggaagaaata atatctcttt tttactcttg tctgttttag
    10921 ccaataatag ctcgtaaagt aatctagaca aacaaataga agtaaacaaa gtttcgtaag
    10981 agcagcaatc gtatcatgtt gctctgctag acgattgacg agcaggaaaa atgttggtaa
    11041 tggaatatag atcacgagta atgaaagaaa aaagtaaaca ataaatggca tactattcct
    11101 cctaattgtg tgagttcgcc ctcattttaa aataacgata tttgtttgtg aagctttttc
    11161 gtgtcgtaac aaatgttatc taatgtattt ttaaataaat aatcattatt atcaaaataa
    11221 tg
//
LOCUS       DAYYFD010000094        10838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000094 DAYYFD010000000
VERSION     DAYYFD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.094"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..1440
                     /gene="lysA"
                     /locus_tag="ACHA25_002374"
     CDS             181..1440
                     /gene="lysA"
                     /locus_tag="ACHA25_002374"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8763644.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1427..2227
                     /gene="dapF"
                     /locus_tag="ACHA25_002375"
     CDS             1427..2227
                     /gene="dapF"
                     /locus_tag="ACHA25_002375"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8763645.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2230..3111
                     /gene="dapA"
                     /locus_tag="ACHA25_002376"
     CDS             2230..3111
                     /gene="dapA"
                     /locus_tag="ACHA25_002376"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8763646.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3108..3824
                     /gene="dapB"
                     /locus_tag="ACHA25_002377"
     CDS             3108..3824
                     /gene="dapB"
                     /locus_tag="ACHA25_002377"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8763647.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3830..4525
                     /gene="dapD"
                     /locus_tag="ACHA25_002378"
     CDS             3830..4525
                     /gene="dapD"
                     /locus_tag="ACHA25_002378"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8763648.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4541..5542
                     /locus_tag="ACHA25_002379"
     CDS             4541..5542
                     /locus_tag="ACHA25_002379"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8763649.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5539..6900
                     /locus_tag="ACHA25_002380"
     CDS             5539..6900
                     /locus_tag="ACHA25_002380"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8763650.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6860..8011
                     /locus_tag="ACHA25_002381"
     CDS             6860..8011
                     /locus_tag="ACHA25_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8763651.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7986..9155
                     /locus_tag="ACHA25_002382"
     CDS             7986..9155
                     /locus_tag="ACHA25_002382"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8763652.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9330..10709
                     /locus_tag="ACHA25_002383"
     CDS             9330..10709
                     /locus_tag="ACHA25_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8763653.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3597 a         1892 c         2422 g         2927 t
ORIGIN      
        1 cttctaatag ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat
       61 atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca
      121 aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa
      181 atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca
      241 cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc
      301 tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt
      361 gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa
      421 atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac
      481 aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat
      541 aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt
      601 atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg
      661 catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag
      721 ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa
      781 atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat
      841 tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat
      901 gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct
      961 ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt
     1021 ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa
     1081 tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa
     1141 tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca
     1201 ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa
     1261 tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac
     1321 tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt
     1381 gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa
     1441 atataatggc tgcggaaatg attttatctt attagattac gcagaagaca cagattatag
     1501 tcaattagcc gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt
     1561 aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg
     1621 cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca
     1681 ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc
     1741 tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa
     1801 tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac
     1861 tagtttattt ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt
     1921 ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt
     1981 caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt
     2041 aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac
     2101 tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc
     2161 tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga
     2221 ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga
     2281 tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat
     2341 ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag
     2401 cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct
     2461 ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca
     2521 gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct
     2581 cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct
     2641 agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc
     2701 attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt
     2761 ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta
     2821 ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta
     2881 gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt
     2941 gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca
     3001 ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa
     3061 aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac
     3121 tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata
     3181 cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc
     3241 cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct
     3301 tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag
     3361 aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca
     3421 tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg
     3481 aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc
     3541 tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa
     3601 aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac
     3661 atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta
     3721 atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag
     3781 gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca
     3841 agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa
     3901 tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt
     3961 tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat
     4021 tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa
     4081 aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa
     4141 tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat
     4201 gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg
     4261 tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga
     4321 agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga
     4381 tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg
     4441 tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt
     4501 gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg
     4561 tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga
     4621 ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt
     4681 attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga
     4741 tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag
     4801 tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt
     4861 aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa
     4921 ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca
     4981 gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga
     5041 tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac
     5101 ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat
     5161 gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt
     5221 acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt
     5281 tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga
     5341 tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt
     5401 tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga
     5461 taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa
     5521 aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg
     5581 atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc
     5641 caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac
     5701 ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa
     5761 aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa
     5821 gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga agattattta
     5881 gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt
     5941 attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca
     6001 gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac
     6061 ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc
     6121 gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga
     6181 atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt
     6241 agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc
     6301 ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa
     6361 gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga
     6421 cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt
     6481 atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca
     6541 gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc
     6601 atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta
     6661 gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg
     6721 ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag
     6781 ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat
     6841 gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga
     6901 aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga
     6961 ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga
     7021 agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct
     7081 ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc
     7141 gatgctttta gggttgactg actttataac taaacacatt gaggatctta tttataatgt
     7201 agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc
     7261 aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact
     7321 agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga
     7381 cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct
     7441 ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga
     7501 gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc
     7561 tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt
     7621 gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg
     7681 ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga
     7741 ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata
     7801 tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca
     7861 tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac
     7921 gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt
     7981 aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa
     8041 aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg
     8101 gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag
     8161 aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta
     8221 tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag
     8281 ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg
     8341 ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg
     8401 aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac
     8461 gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc
     8521 ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat
     8581 atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata
     8641 tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac
     8701 atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac
     8761 taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg
     8821 ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac
     8881 gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg
     8941 gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt
     9001 gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag
     9061 aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct
     9121 gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa
     9181 tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg
     9241 aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt
     9301 ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg
     9361 catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag
     9421 atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt
     9481 atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc
     9541 cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa
     9601 aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat
     9661 tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa
     9721 atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc
     9781 cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag
     9841 caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca
     9901 cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg
     9961 aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca
    10021 aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa
    10081 atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg
    10141 aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt
    10201 ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac
    10261 gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag
    10321 gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta
    10381 cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa
    10441 aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa
    10501 tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa
    10561 atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact
    10621 tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag
    10681 cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa
    10741 aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
    10801 acttggctgg acttattacc gcagatgcaa gcttttga
//
LOCUS       DAYYFD010000095        10552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000095 DAYYFD010000000
VERSION     DAYYFD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.095"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACHA25_002384"
     tRNA            8..81
                     /locus_tag="ACHA25_002384"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACHA25_002385"
     tRNA            101..173
                     /locus_tag="ACHA25_002385"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACHA25_002386"
     tRNA            201..274
                     /locus_tag="ACHA25_002386"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACHA25_002387"
     tRNA            288..361
                     /locus_tag="ACHA25_002387"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACHA25_002388"
     tRNA            385..474
                     /locus_tag="ACHA25_002388"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACHA25_002389"
     tRNA            487..560
                     /locus_tag="ACHA25_002389"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACHA25_002390"
     tRNA            579..654
                     /locus_tag="ACHA25_002390"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACHA25_002391"
     tRNA            656..726
                     /locus_tag="ACHA25_002391"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACHA25_002392"
     tRNA            740..813
                     /locus_tag="ACHA25_002392"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACHA25_002393"
     tRNA            838..925
                     /locus_tag="ACHA25_002393"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACHA25_002394"
     tRNA            947..1018
                     /locus_tag="ACHA25_002394"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACHA25_002395"
     tRNA            1041..1130
                     /locus_tag="ACHA25_002395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACHA25_002396"
     tRNA            1143..1216
                     /locus_tag="ACHA25_002396"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACHA25_002397"
     tRNA            1235..1307
                     /locus_tag="ACHA25_002397"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACHA25_002398"
     tRNA            1311..1383
                     /locus_tag="ACHA25_002398"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACHA25_002399"
     tRNA            1388..1458
                     /locus_tag="ACHA25_002399"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACHA25_002400"
     tRNA            1471..1544
                     /locus_tag="ACHA25_002400"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACHA25_002401"
     tRNA            1556..1627
                     /locus_tag="ACHA25_002401"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACHA25_002402"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACHA25_002402"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8763654.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACHA25_002403"
     CDS             3216..4688
                     /locus_tag="ACHA25_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8763655.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACHA25_002404"
     CDS             4771..5559
                     /locus_tag="ACHA25_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8763656.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACHA25_002405"
     CDS             complement(5626..6294)
                     /locus_tag="ACHA25_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8763657.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACHA25_002406"
     CDS             6409..8121
                     /locus_tag="ACHA25_002406"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763658.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="ACHA25_002407"
     CDS             8124..9896
                     /locus_tag="ACHA25_002407"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763659.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(9978..>10552)
                     /locus_tag="ACHA25_002408"
     CDS             complement(9978..>10552)
                     /locus_tag="ACHA25_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8763660.1"
                     /translation="VQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDG
                     NKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFI
                     SKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGH
                     EDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3084 a         1855 c         2363 g         3250 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag ttgattttgc
    10021 atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc atgtcctaca
    10081 cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat gtgggaatgc
    10141 cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac tgctcttaaa
    10201 gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa aatgtattta
    10261 tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat ttctttagca
    10321 ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt tttaattttt
    10381 aactcgtaat cataagtacc agaaaccgta attttattac catcataatc agattcagtc
    10441 aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt aattaaagaa
    10501 taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac tt
//
LOCUS       DAYYFD010000096        10543 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000096 DAYYFD010000000
VERSION     DAYYFD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.096"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..1067
                     /locus_tag="ACHA25_002409"
     CDS             171..1067
                     /locus_tag="ACHA25_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8763661.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1217..2158)
                     /locus_tag="ACHA25_002410"
     CDS             complement(1217..2158)
                     /locus_tag="ACHA25_002410"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8763662.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2284..3405
                     /locus_tag="ACHA25_002411"
     CDS             2284..3405
                     /locus_tag="ACHA25_002411"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8763663.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3663..5165
                     /gene="eat(A)"
                     /locus_tag="ACHA25_002412"
     CDS             3663..5165
                     /gene="eat(A)"
                     /locus_tag="ACHA25_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8763664.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5232..5759
                     /locus_tag="ACHA25_002413"
     CDS             5232..5759
                     /locus_tag="ACHA25_002413"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8763665.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5970..7031
                     /locus_tag="ACHA25_002414"
     CDS             5970..7031
                     /locus_tag="ACHA25_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8763666.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7034..7930
                     /gene="murQ"
                     /locus_tag="ACHA25_002415"
     CDS             7034..7930
                     /gene="murQ"
                     /locus_tag="ACHA25_002415"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8763667.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7944..9926
                     /locus_tag="ACHA25_002416"
     CDS             7944..9926
                     /locus_tag="ACHA25_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8763668.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9991..10437)
                     /gene="nrdI"
                     /locus_tag="ACHA25_002417"
     CDS             complement(9991..10437)
                     /gene="nrdI"
                     /locus_tag="ACHA25_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8763669.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3444 a         1858 c         2279 g         2962 t
ORIGIN      
        1 caactattca gaaaactttt aggcgaattt taatagaaga atgatttatt tcatcatatt
       61 ttcatacaaa tgttaaattt tcatacaaat ataggaaacg ttttactggc tttttactat
      121 ttaaacaggc tatcatagga aaaagtgaca gagaaagtga ggcggaaaag atgaacaaaa
      181 atgacgtact gctagttgca aaaaataaat cactttattt tttgccttcc atccgctctt
      241 tagatgtttg gtccggaaaa gatttactac agtcggaatt cattaaatca gctagacaac
      301 agttggcaaa ttatcagctg atcgtttttt tagattatgg atttgatatg gagatggctc
      361 gacgcgtgcg cccatttacc aatgcaaaaa ttgtgttatt tttttggaat cacttcaaag
      421 cagaacacga ggcgatgctg aagcaggcac aagatgaacc ggcaatcgat gagatttatc
      481 attttgattt tttggaagca aaagaacttg ggctgaaaca taatagttca ttttattcga
      541 aacatatgaa tttttcttct agcgaaccaa agacggatct attttttggt gcaacgaata
      601 atgggagaaa agaacgagca gaaagctata agcaggaatt tataaaaaga gatgttacga
      661 ccaactattt cattcttcca catcgaggga atgaacaacc tggatatctt acttatgaag
      721 agtacctgaa aaaaacaggg gaatcgcgcg ggattctgga actgttgcgg gagggtcagc
      781 aaggagtgac tttgcggaca tttgaatcca tgtattttca aaagaaacta gtgacggata
      841 atcaagcgat tgctcattat catttttatc gtcctgaaaa tatttttttg cttcgagaac
      901 gtgatttaga tgaattaccg gaatttctgc atacaccatt taagccaatc ctatcagagc
      961 tattggaatt ttatgatgca gaaaattggg caaaacgatt tatcactaac gattccacta
     1021 tttatgagca atacgaatat gacctctcct ctgttgaaaa caaataaaaa acctacattt
     1081 tgaagagatt atgtactgaa acaaacaagc acaattctta tggcatcatg agagttgtgt
     1141 tttctttttg atcttttatt gggcttaaaa aaagagaacc agctgctgat tcagctgatt
     1201 ctcatcagag agccttttat tctgcgccaa ggattccttc ttttgcatgg actaattggg
     1261 tcaaaaaggc acaatatggt gtagcaatat catatttttg tccttttcgc caaatggcac
     1321 cattgatgta gtcgatttca gtcaaacgat ggtttttgat caggtcttgg tacatagatg
     1381 ggtaatgcaa cccgatgttg tcaggatcat aagtagaagc aatatgttgg tacacttctt
     1441 cttgatctag tatgattcct tcttttgctg cgatagaagc aaattcagat acgattgttc
     1501 gaaccatgct ctctgccgct ttttgcgcgc ctagttcagc aatattacaa tccaaaatcg
     1561 tgcataaacc attcaatgtt ccatttacac aagcttttcg ccaaatggaa taacgaacgt
     1621 tatggctata gatcggattt aaatttgctt cttgaaagac ttctacaact ttttttgtaa
     1681 atgcttcgcc ttctggttcc aagttctcta gttcgatttc tccatcacct aacaatgtga
     1741 cttttccggg accagctaga ccagctgtcc acatcgtgat cccaaataag atatttttct
     1801 ttggcacata tttttccagt acatcttcat gacctaatcc atttagtaaa cacaaaacat
     1861 aagtattgtc agtaataatg gattggatcg aacaaaacat atcatctaat tgattggctt
     1921 ttgttaatgc tacgatcaag tccacgtgtt cgttgctttc gatgatttct tctggtgaat
     1981 agattggcag ttttgcgacc acttctttac cgttaaagtc agcaatcaac ccatttgtac
     2041 gaatggcttc aatgtgagca ggccaacgat cgatcaagag gacctcattt ccgctttgat
     2101 gtagcatcaa tccgattcgg ctgcccattg ctccggcccc agctattgcg attttcatgt
     2161 tttttctctc ctttattcaa tgagttccct tttagtatat agaaaaatgg aagaaaatag
     2221 cattgttttg tttgaaaaaa atgaaaaaaa gtgtcttact taagaagaaa gtgagggata
     2281 cagatggata ttttggcggc aattgattca tttaaaggaa gtgcttcttc atttgaacta
     2341 aatcaggcag ttctaaatgg ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt
     2401 gcagatggcg gagaaaatac tgcagaagca atctattacg cgctcagggg aacatggcgt
     2461 accattgatg ggatggatct cttattccgt cagcgaaaaa tcagctattt attgacaact
     2521 tggcaaggaa aaaacaccgc agtcatcgaa tctgcacaag cattggggtt agatctactt
     2581 gataaaccaa cggatcaaac tatccgactt gcttccagtt atgccttagg agaacttatt
     2641 aaagatgcac tgcttcaaga agtagaacaa attattgtca ctttaggagg aagcgggtgt
     2701 agcgatggtg gtctaggatt actccaatcc ttgggtgctt ctttagaagg gtacacagaa
     2761 ggaaatcctt tattatctac taaaaaactt cattttgatc gaagaaacgt tcttgtttct
     2821 aaagtaaaat tgattatcgc tgcagatgtt accaatccat atacaggtca acaaggagct
     2881 gcttggcaat ttggaagaca aaaaggagga atagatacga cgctagcttt cttagataaa
     2941 caagcaggaa agattgctca gcaaataaaa gatttctggg aggtggattt agatgatatt
     3001 ccaggaagtg gagcagcagg cggtattggt ggagcccttt ttttgttggg agcgaagatg
     3061 gtttctggtt tcgatttagt cagccaaatc actggattgg aaaaaaagat cgaacaagca
     3121 gatgtcatca ttacaggaga agggcggatc gatcagcaaa cgatccacgg aaaagttcca
     3181 tacggtgtag cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt
     3241 agagaccaag agctagaagg actttctgat tttctcccaa ttgtcttaag tattcatttg
     3301 ggtccaatag gtttagaaga agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc
     3361 ttaggacaga cacttagcag atttttcacc atcctcccga agtagttgac aaaatcattt
     3421 tctttatgct atcgtaattt caaatatagg acaaagtgag ggatccgtgc attgaaaaat
     3481 taagtcgatt gatgaaagct gcattcagac tgtaccttgt ggagacaagg aagcagtgtg
     3541 cagattttcg ggattggctt ggttttttcg atgtatgggt ctttttgtgt gtatccaacc
     3601 cttgcatcct ctttagccag ttctgaaaat aggactggtc tttttttacg aggtgatgga
     3661 aaatgtctaa aatcgaaata aaaaatctga cattcggcta cgacagccaa ggcacattat
     3721 tatttgaaca agcaaatcta aattttgaca cacaatggaa actaggactt atcggacgaa
     3781 acggtcgagg aaagacaact ttactgaata ttctacaaaa caaactacct tatcaagggc
     3841 aagtaatcca tcagcaagaa tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt
     3901 taacctatta cgtgttaaat gatattacgg attttgagat atgggaaatc gaaagagagc
     3961 tccaattgat gcaaacagat cctgaaatct tatggagaga attcagcaca ctatcggggg
     4021 gagagaagac aaaagtccta ctggcacttt tatttgtgga tgacactcat ttcccgttaa
     4081 tcgatgaacc aacgaatcat ttggatatct ctggtagaaa acaagtagcg gcttatttga
     4141 aaaagaaaaa acaaggcttc atcgtggtca gccatgaccg gggatttatc gatgaagtag
     4201 tggaccatgt tttagcaatc gaaaaaagtc aactggaact ttatcaaggg aatttctcta
     4261 tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga
     4321 aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa agccgaatgg tctcgttccc
     4381 gagaaggaga taaaacaaag aaacaagtcg gattcatcga tactgaatct agacgagtga
     4441 ataaaggagc agtgggtgct gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga
     4501 atcggatgga gacccagatc agcgagaaag aaaaactatt aaaagatatc gaatatatcg
     4561 attctttgac gatgaatagc caagcgtctc accataagcg acttttaagc gtagaagatc
     4621 ttcaattagg gtatgaaaat ctgttattcg agccaattca ttttacaatc gagcctcatc
     4681 agcgggtggc gatttcaggt cctaacggtg caggaaagtc atccattatc cattatcttc
     4741 tgggggcatt caacggcaag gttataggag aaaaatacca gccaaaacat ctgagcatta
     4801 gttatgcaag ccaaaattat gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa
     4861 accaagtaga ctaccaagca tttttgaaca acctccgaaa gcttgggatg gaaagagatg
     4921 tttttcataa caagatcgag cagatgagta tgggccaacg gaaaaaagtg gaattggcta
     4981 aatctttatc acagccagct gaactatata catgggatga accattgaat tatttggatg
     5041 tcttcaatca agaacaatta gaacaactga tcttgaacgt gaaacctgcc atgttactag
     5101 tggaacatga tcaaaccttc ctggataaag tatctactga gattatttct cttgagagaa
     5161 tctaaccctt gtaatctcaa gaacaacaag gtatggttaa ggggaacttt gccaacgaag
     5221 gagagaatca catgctttat gtaacgagac acggagaaac aacttggaac gcacaaggat
     5281 tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg acagatgcag gcacagaaac
     5341 tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa aatcatccat tcccctcttc
     5401 aacgagcaag agatacagca caagcagttg ctgatcgtct cagcttgccg ctgaccgttg
     5461 atgaacgatt ggtagaaatg gattttgggg attatgatgg actgccaagc aaagatgaga
     5521 actttcaaaa agcacgttta gcgttcgctg tacgattccc aaatggggag tcagtcttag
     5581 atgtgtatgc acggatcgtt cctttattga aagaatgcat cgaagacgaa gaaaatgtct
     5641 atctgttagt ttgccataat gccttgataa gagtgatcaa tgcttatttt catccgatgc
     5701 ctaatgaggg gttctttaca ttcatggtag ataatacaga attggtttct tatgagtaat
     5761 tatgtttttg tgttatttaa ataaaatatt tcaaggcact gcttttgctt agcagtgcct
     5821 tttttaggtt atgacaattc ttttgataat ataaactcaa cctttagaaa tgaaatttaa
     5881 ttattttaat aaaagtattg aaattttgtt tctttagata tatactctag tcatagaaat
     5941 gaagacggat acagaaaaag agggataata tgtttggctt ttcaatattt atgaacgatt
     6001 ctttgtcaga agagaagaaa cagtatatcc gccaaatggc atccaatggc tttgtcggta
     6061 ttttcacttc catgcatata ccagaagatg atgtttcggc atatcaaaag cgattgatcg
     6121 aactaggaag cgaagcgaaa aagaataaac tagaattgat ggtcgatatt tcaggagagg
     6181 cattggaacg agcgggtttt tcattagaag atctaacgcc tttgaaagca attggtgtaa
     6241 caggtttaag aatggattat catatttcca atcagcagat tgccgactgg tcacatcaat
     6301 tgaaaatcag tttgaatgct agcacgatca cgcctaaaga tatcgatgaa ttgaaagaag
     6361 cagaagctga tttctcacag attgaagctt ggcataatta ttatccacgt cccgaaacag
     6421 ggctggacaa agaatggtat cagaaaaaaa atcaatggct caaaattcaa ggtctccttg
     6481 tccaaggttt tgtaccagga gatacagagt tgagaggccc actatatcaa ggattgccaa
     6541 ctttggaaga acatcgaggg atccatcctc ttgcaggcgc tctagatcta ttagcaagta
     6601 acacggatat tgtttacatc ggagatgcag gtctgtcaga aaacgttcag gagcaatttg
     6661 cttctttcca aaaagaacag acagtcttgc tccatacgga acctgtagat gaagaatttt
     6721 acgaatatat attagggaag catacgaatc ggcaagacga tgcaagagat gtcatacgaa
     6781 gcgtagatgc acgttttcga gagattccgc ctatcccagc aagaaataca gctacgcgta
     6841 tgaaagggtc tattacttta gataacgaaa agtatttgcg ctatatgggt gagatccaat
     6901 taaccaaata tgatttgcca gcggatgaaa aagtgaatgt agtagctaaa gttatcaaag
     6961 aagagctacc attgatcaac caaatcaaag ctggtatgaa ctatcaattt ataagaaaag
     7021 aaggaagata aaaatgagca aaatcaattt agagaaccta acaacggaac gcagaaacca
     7081 agaaactttt ggattagatg agatgagtat ttctcaagcc gttcaactaa tgaacaaaga
     7141 agatcagaaa gtggccgctg cggttgaaaa agaactgaca agtatcgaac cagtgattgc
     7201 taacacgatt gagtccttta aaaaaggtgg acgtctgatt tatatgggag ccggaacaag
     7261 tggaagacta ggtgtgttag atgctgctga atgcgtaccg acattcggtg tagaaccgga
     7321 aatggtcata ggtctaattg ctggtggaga agaagcaatg actgtagctg tcgaaggggc
     7381 cgaagattct tatgagctag ggcaagaaga tctaatcaag ctgaatttga caaaaaatga
     7441 tatagttatt ggtatcgcag ctagcggtcg tacaccatat gtgatcggtg gattagatta
     7501 cgcaaaatcg atcggtgctg tcacaggaac gatttcttgc aataaacaag ccgaaatcag
     7561 taaacacgcg gatttcccaa tcgaagtcga tgcaggtcca gaatttttga caggctctac
     7621 tcgattgaag tcaggaacag cacaaaaact tatcttgaat atgatttcta ctatcagtat
     7681 gattggtata ggtaaagtat tcaataactt gatggtcgat gtcaaaccaa caaatgaaaa
     7741 actaatcgaa cgctcaaaac gaatcatcat gcaagcgaca gaagctgatt acgaaacagc
     7801 tgaaaagtac tttgaagaag cagatcaaaa tgttaagctg gctatcgtca tgattttgac
     7861 aaatagtgat aaagaggaag ctcaagcaaa attagctgaa gcaaacggat ttgtaaaagg
     7921 gaccttatga gggggaaaag accatggcaa aaaacgaact gactttggaa gaactggcaa
     7981 aacaaattta taaaggggca ggcggcatgg gcaatgtaga aagcattgtc cactgcatga
     8041 ctcgtgtgcg tatgaagatc atcaatgatg atcttgtaaa tgtctctgaa ttaaaagcga
     8101 ttccaggagt gcttggtgta gtagaagatg aacagcttca agtcatcatc ggacctggga
     8161 aagtgaacaa agtagcacag caaatggttg atttggcagg cgtgaaatta ggagaaaaac
     8221 tttcttctgt aagtacagct acagcaaatt catcagcatc tggaaaagac cgtgtaaacg
     8281 aacgggcaca agaaatgaaa gcggcacaaa aatcgaaaca aaaaccatct aaaatcaaat
     8341 cggtattaaa agacatttct aatatctttg tgccaatgat tcctgcattt gtcggtactg
     8401 ggatcgtggc agggattgcc gcagttttag caaacttagt cacagccgga acacttgatg
     8461 cagcaacatg gcaacaatat atcgatgtga tgaacatctt gaaaaacggg atgtttagct
     8521 acctagttat ctatacaggt atcaatgctg cacaagtttt tggtgcaaca ccaactcttg
     8581 gtggagttat cggggccgtt gtgatgctga cagggatgaa tccggaagca ccactgaaaa
     8641 acttatttac tggtgaagct cttgcagctg gacaaggtgg gatcctaggt gtcatcttcg
     8701 ctgtttggct attgtcaatc gttgagaaaa aactgcataa agtagtacct gatgcggtag
     8761 atatcatcgt tacaccaact ctttcattga ttgccatcgg tctgattgaa attttcttca
     8821 tcatgccttt agcaggtttc atttctgatg gcatggtcgg tgggatcaac tgggtacttg
     8881 gcgttggtgg agcatttgcc ggcttcgttc taggcacatt attcttgccg atggttatgt
     8941 tcggactgca tcaaattttg actccaatcc atgtgcaaat gattgatgaa actggtcgta
     9001 cgctattgct tccaattctt gcgatggctg gtgcaggaca agtgggtgca gcattggcat
     9061 tatggattcg ctgcaaaaaa gataaagaat taacagaaat gatcaaagga gctttaccag
     9121 tcgggatcct aggaatcggc gaaccattga tttacggagt aacattacct ctaggacgcc
     9181 catttattac tgcttgtatc ggtggaggta tcggcggggc agtgatcgga atgatcggaa
     9241 atgtcggggc tatcgctatt ggaccttcag gagccgcttt gatcccattg atctctgacg
     9301 gtaaatggtt aggctacatc cttggattat tggcagctta tgctggtggc ttcgttgcta
     9361 cattcttctt cggtattcct aaagaccgtt tagaaaaaga agaattagcg actgaaacag
     9421 ccacagtaaa tgaagtgcct gaaaaagtag tacctcaaac ttcagctgaa agtgtgacta
     9481 tcctctcatc agttgctgac ggaacagttg tttctttaga taaagcaagt gatccagttt
     9541 ttgcacaaaa gatgatgggt gaaggctact ttgtagaacc agaaaacgga catatctatt
     9601 ctccagttac tggggtagtc agctctgtct tcccaacgaa acacgctatt gggatcacaa
     9661 cgccaaatgg tttagaaatc ttgcttcata tgggaatcaa cacagttgac ttaggcggta
     9721 cgccatttga tgtgaaagta acagaagggc agcaagtgac acctgatacg cttgtagcag
     9781 aagctgactt agaagcgatc aaagcagcag gcaaagaaac atccatgatc gtcttggtaa
     9841 ccaatatgga tcgtgtaaaa aactttgtct tggaaaaaac aggtaaagca aaagcaaaag
     9901 cacctgtgat ggatgtcaaa gcttaatatg atagaaacaa tagaaacgaa agaagaaact
     9961 gaaatctatt tttcagcttc ttctttttta ttatttattg ttttctgcta aaatctgata
    10021 aattcgtgtc acatcagatg gtgttccccg taattcataa tctgccaaga aaggaaagcc
    10081 aaatgtttgt gcatattgtt tggctgttag acaatactga ttattgaagt ttttatttcc
    10141 gctaccgact acaccaagac aaagtttatg attgtcttca tgttccagat attcacgcat
    10201 cgtttcggtc aagatttctg tatcaccgtt atctaaacca tttccgccgt ctaaataagt
    10261 aggaacgaaa gtaaaaaatg gttctgtttc tttcgtaaaa tcactatttt catggatttc
    10321 ttttaaagta acttcaggat tgacaggatc ttctgcatgc attttttcag catattctgc
    10381 tagatgtttg gcaaaggcac gcgtatttcc tgaaatcgat atatataaaa tgttcatgga
    10441 tcatcctcca attctttcct tttagtatag catctttttt aagttttttg gtggtgtaat
    10501 ttggaaaggg actaggcgct ttgcttgtat aatgagaaaa aag
//
LOCUS       DAYYFD010000097        10382 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000097 DAYYFD010000000
VERSION     DAYYFD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.097"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHA25_002418"
     CDS             complement(79..2199)
                     /locus_tag="ACHA25_002418"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8763670.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHA25_002419"
     CDS             2429..3607
                     /locus_tag="ACHA25_002419"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8763671.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHA25_002420"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHA25_002420"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8763672.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHA25_002421"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHA25_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8763673.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHA25_002422"
     CDS             complement(4960..6213)
                     /locus_tag="ACHA25_002422"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8763674.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHA25_002423"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHA25_002423"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8763675.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHA25_002424"
     CDS             7790..9136
                     /locus_tag="ACHA25_002424"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8763676.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHA25_002425"
     CDS             complement(9444..9830)
                     /locus_tag="ACHA25_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8763677.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHA25_002426"
     CDS             complement(10098..10247)
                     /locus_tag="ACHA25_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763678.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3308 a         1874 c         2094 g         3106 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 ct
//
LOCUS       DAYYFD010000098         9717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000098 DAYYFD010000000
VERSION     DAYYFD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.099"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            347..1396
                     /gene="trpX"
                     /locus_tag="ACHA25_002427"
     CDS             347..1396
                     /gene="trpX"
                     /locus_tag="ACHA25_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8763679.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1393..2274
                     /locus_tag="ACHA25_002428"
     CDS             1393..2274
                     /locus_tag="ACHA25_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8763680.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2277..3047
                     /locus_tag="ACHA25_002429"
     CDS             2277..3047
                     /locus_tag="ACHA25_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763681.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3326..4180
                     /gene="aroE"
                     /locus_tag="ACHA25_002430"
     CDS             3326..4180
                     /gene="aroE"
                     /locus_tag="ACHA25_002430"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8763682.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4358..5521
                     /locus_tag="ACHA25_002431"
     CDS             4358..5521
                     /locus_tag="ACHA25_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8763683.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5638..6048
                     /locus_tag="ACHA25_002432"
     CDS             5638..6048
                     /locus_tag="ACHA25_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8763684.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6222..8450
                     /gene="topB"
                     /locus_tag="ACHA25_002433"
     CDS             6222..8450
                     /gene="topB"
                     /locus_tag="ACHA25_002433"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8763685.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8621..8905
                     /locus_tag="ACHA25_002434"
     CDS             8621..8905
                     /locus_tag="ACHA25_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763686.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9065..9301
                     /locus_tag="ACHA25_002435"
     CDS             9065..9301
                     /locus_tag="ACHA25_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8763687.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9298..9696
                     /locus_tag="ACHA25_002436"
     CDS             9298..9696
                     /locus_tag="ACHA25_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8763688.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3377 a         1649 c         2065 g         2626 t
ORIGIN      
        1 agttgacgaa attcaaaaat tcgattagac ttaaatcaat tcttaaggaa agggtgacct
       61 gtaatgaact tatcaaaacg tatttcagca gaaaacaaaa aaaattggcg cagctggcgt
      121 agataagttg tattaatggt gagactttcc catagataca actttttaga gtgcatttct
      181 aaagcttgta tctatgctag acatgaacat tgactgccgc atagattaga atctatgcgg
      241 ttttttgtgt aaagacaatg accgctttgg ccattgtctt tttttgtaga ctggatactt
      301 gcctgattat aataaataaa aagattgact ttgcaggagg aaaaaaatga aaaacaaacg
      361 attaataaca gttgtagtaa ttattttagt ttatttagca ggtgcattta tttatggaaa
      421 agcctcaggc gaaaaagaaa gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg
      481 ggtattgcaa tacgtgagcc atccggcatt agatgagatt tatcgaggaa tcaaagatgg
      541 tctgaaagaa gaaggattaa aagaaggaga aaatcttgag atcaaatttc aaaatggaca
      601 agccgatcag agcaagctag ccacaatgag tcaacagcta gtacaatctg atccagatgc
      661 cttgattgga atcgcaacac ctgccgctca atcattagca aacacgactt catcgatccc
      721 agttgtgtta ggtgctgtaa cggatcctgt gggggctgga ttagtcgctt ctttagagaa
      781 acctggtggt aatgtaacag gtgtttctga tcagccgcct gttacttcac aaatcaagct
      841 tgcttctgat ttgttgccaa atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga
      901 taattcaaaa taccaagtag cacaagcaga agaagctgca aaaaaagaag ggttagaatc
      961 agtaaaatat gcggtgcctt ccactaatga gattgcacaa acggttcaag tgatgagccg
     1021 acaagtcgat tttatctatg ttccattaga taataccatt gccaatgcga tgcaaacagt
     1081 ggtaaaagaa gcaaacaaag caaatatccc cgtcattcca tctgtggata caatggtgga
     1141 acaaggtgga ctagcaacaa tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa
     1201 gatggcggca gaacttgcga gtggaaaaga aaaaccggaa acgacgcctg tttatacatt
     1261 tgatcaaggg gatacagtga tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc
     1321 ccaatcgatg aaggaaaaag ccaaaatcat tacagatgac tcgcagcaag aaacatctaa
     1381 ggaggatgac aaatgatcgt atcagctatt tcacaaggca tgctttgggc aatattagga
     1441 ttagggattt tcatgactta tcggatctta gactttcctg atatgacgac agaagggtcc
     1501 ttcccattag gtgctgctgt ctgtgtgaca gctattactc atggggtagc gccgattgta
     1561 gctacattat taggcgtagg tgctggcatg atagcaggac tcgttacggg gttactttat
     1621 acaaaaggaa agataccggt tattttagca gggattttag tgatgtcagg attgaactca
     1681 gttattttgt ttgtgatgcg aacaccaaat ctttctttat tgaatcatcc gattctacaa
     1741 gacagcttcc atcgctttgg gttacctgat tactttgaca cagtccttct tggcttcatc
     1801 tctttgggca tcttgattgc tgtactgcta ttcttcttca atacagattt gggtcaaggc
     1861 tatattgcga caggagacaa cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt
     1921 atgaaaattt taggtttaac tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt
     1981 gcccagaacg acggatatgc agatgtaaat aaagggattg gcgtaatcgt catcggtctt
     2041 gcttccatca tcatcggtga agtgatcttc caagaattga cgctagcaga aagattgatt
     2101 gctattgttg tcggcagtat catttatcag ctattgattt tagtagtaat caaactaggg
     2161 tttgacacga cttatctgaa gttgttctcc gctatcatct tagctatttg tctgatgatc
     2221 ccgcaattga agaaagcact gaacttaaaa acgggttttg agaaggaggc atagtaatga
     2281 ctgatatatt gacaatcaaa aatggcagaa aagtcgtaaa caatggaatg aatgaagaga
     2341 aagtactatt ggatgaaatt aaccttacaa tcagagaagg ggacttcatc actgtccttg
     2401 gcgggaatgg tgctggaaaa agcacactgt tcaatacgat cgctggtacg cttcaactca
     2461 gtcaaggatc gatctctttt aaagatcagc tgatcacaaa agattcagaa gaaaaaagag
     2521 cacgattcct ttctcgagtt ttccaagacc ccaagatggg aacggctcct cggatgacag
     2581 tagcagaaaa tttgctactt gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta
     2641 acttgaaaaa tcaaaaagaa gattttttca gactatgtca agaagtcggt aatggcctag
     2701 agcaacactt ggatactcca gcgggtgaat tatctggtgg tcaacgccaa gcattaagct
     2761 tgctgatggc gacgatcaca gctcctgagt tactattatt agatgaacat actgcagctt
     2821 tagatcctaa aacagctaaa gcattaatgc gattaacaaa tcagcggatc aatgaacaaa
     2881 aattgacttg tctcatgatc acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc
     2941 tgatcgtact ccaaaaaggg aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt
     3001 tgacaatggg cgaccttttg cagttctttg aagaagtagt ggattgattt cgacagaaaa
     3061 attcctttca gtagaaggac ttgaaaactc attgacatct tcctttgatt catcctatac
     3121 ttaaaacaat aaacaattaa acatatttgg gtgttttgta ggatcacgta gtcaatgatt
     3181 ttaacaatta aatagaatat tctttgctct gacaagtaaa cgatgagaaa agaatttgct
     3241 actttgccac ctagacgttt cgtacttttt cgtatcaaac gctaagtggc tttttttatt
     3301 gaagtaagaa caaaggagga ataagatgat ttctggacaa accaaattag ctgggctctt
     3361 tgctgatccg gcttctcaca gtttatcccc attgatgcac aatactgctt ttgaagcaaa
     3421 caacatcgat gcggtgtatt tagctttcca agtaaatcca atgaatttga agcaagcagt
     3481 cgaaagtatt cgaacttttg atatgttagg tgtaaatctt tcgatgccga ataaaacagc
     3541 ggttattcct tatctggatg aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac
     3601 gatcgtgcac gaaaagggtc gtttgattgg ctataatacc gatggtgcgg gcttcatgca
     3661 atccgtgcaa gaagctggga tttcgatcaa aaagaaaaaa atgactctac tgggagctgg
     3721 aggtgcagca aaggcagtca ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt
     3781 ttataaacga aacaaccaaa cattcgctca agtaaaagaa acattcgaac aactcagcta
     3841 tcgaacttct tgtaagataa aagttcgtga ctttgccgat acgcatcaat taagaaaaga
     3901 cttgaaagaa agctgtcttc tagtcaatgc aacagatatc gggatgggaa agaaaatcaa
     3961 tcagtcaccg ctcttagata taagcatgat gcatgagaat ctagcggttt ttgatttgat
     4021 ttatgcacct agagaaacac gtttattaaa agaagcaaaa aaagtaggag caaaaggata
     4081 caatggactt ggcatgttga tctaccaagg agcaatcgcg tttgaattat ggacaaagga
     4141 aaagatgccg atccatgtga ttgaaaagct gtttgattaa aaaaggataa tagataaaga
     4201 tcttttattc tcgcataaac ggtctcaaga aaagtaatcc tttatgtatc gaagcattta
     4261 tttcgttatt ttttattaaa ttatcaaaaa atgataggct tttgtcgatt ggttatcgta
     4321 tcctttattt atcagtaaga aacgaggagg aaccacaatg agtcggatgg atcgctataa
     4381 ggacatccat aagaaggcga aacctctgaa gcgggatacg gggagtattt ttgctagaag
     4441 agaacgaaaa cgaacgcgta tggaaaatga ggaagatgct acccgtattt atgaaagaga
     4501 gcagcattca tttgaacatt tgaaccaata cgagcaacag atatacagag atgcaccaag
     4561 agcagaagaa caacaaaaaa ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt
     4621 aaaacctcga agaaagcgat cgtggaaaaa aatcattcta tcgattttgc ttgttttgat
     4681 cctttattca ggtatctcat tttttattgg taaatatgta gctgaacatg acacaagctt
     4741 agaggcagcg acttcagaaa cctttcatgg gcaaaaagcg acgaatggtg cgcataatat
     4801 tttgatcctt ggaagtgata cacgcggaga agatgccggg cgagccgata cgatcatggt
     4861 cttacagttg gatggtcctt cccataagcc gaaactgatt tcttttatgc gtgacagctt
     4921 cgttacgatc ccaggggtag gacaaaataa gatcaattca gcttatgcat acggaggagc
     4981 cgatctagtg cgtcaaactt tagtagaaaa ttttggaatc gattgtcaat actatgcgaa
     5041 agtcgatttc aaatcgtttg aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat
     5101 cgatgcagaa aaagatctga atctggatgg tgtagatatt aaaaaagggg tccagaagat
     5161 ggatggacat gttcttttgc agtacgcacg tttccgaatg gatgaacaag gagactttgg
     5221 ccgtgtcaga cgccaacagc aagtaatgaa cgctattttc agccaattga agaatccatt
     5281 gaatctgatc cgtgcacctt atgcagctgg taaagcttta ggatatacat ccacagatgt
     5341 cactgcttgg ttcttgacga aaaacctctt ttcgattgca cgaggagctg gtggcattga
     5401 tcgaataagt gtgccagtag acggttcgtg gaattatggg caaagtgatt atgctggcag
     5461 tattcttgaa attgataatc aaatgaatca atctgctatt acagattttt tgaataaatg
     5521 atcataaaaa agatcaagaa gaagctgcgc aaggcttttg tcatgaatca atcaaacgat
     5581 tcactcgtaa aaaacgcttt cttatggtag atttaaccaa gagaggagct gtttacattg
     5641 gatatctatc tgaacttcaa aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt
     5701 tttcagactg aagcatcagg tatcaccaca tacggtgaaa tgcctggaat agaacaaatg
     5761 aacttgccag aagaagcaaa aaatctaatc acgaatgcac ggctgaacat ttatgataca
     5821 ttagttatgt ttgcagatac gccagaagga attggtcctg aattagttgt gggaaataat
     5881 gtttctttag tcatccagac aagaaatgaa gaagaaacga agcgcctatt tgaaagctta
     5941 gctgagggtg gtaagattga gatgccactg gatgctgttc cgtgggctca gttgtatggg
     6001 atggtaactg atcgatttgg catcaactgg cagattaacc aagagtgaca gctaaagatg
     6061 aatattttct ggaaacatca agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg
     6121 tgtctatttc gactatacgg tatctttttt ttattgccag atgcgaaaat atgttccata
     6181 atagagaagt ccgcaaaaaa ttaagtaacg gaggtcagaa gatgaagacg gtgatactag
     6241 ctgaaaaacc ttcacaagca aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg
     6301 aagggtatta tgaaatcagc gatccattgt ttgctggtga agtaaccatc acttatggtt
     6361 ttggacattt ggtagatatg gttgctccag gagcttatga agagcgttgg gcaaaatggt
     6421 ctttagaaaa tttgcctatt ttcccagaaa ccttccgtta cgaagttcca atggacaaac
     6481 gtgcacaatt ttcgatcgta aaacgagaat tgcagtcagc cgataccatc attattgcaa
     6541 cagatggtga tcgagaaggt gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat
     6601 tttctaagga aaagaactat ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct
     6661 atgaaggttt taagaatctc caacctggaa agaactattt tccaaaatat aaagaagcgc
     6721 aagcaaggca gaacgctgat tggctgattg gaatgaatgg aagtccatta tatagcttgc
     6781 tacttcaacg aaaagggatt gatggcagtt tttctcttgg tcgtgtacaa acaccgacac
     6841 tctatatgat ttatcagctg caagaagaaa tcaaacactt caaaaaagaa acgtactttg
     6901 aaggagaagg tttaattacg acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa
     6961 aagcatttaa aacccaagaa gagttaacga aggaaataga aaaacttgga gcgcatttag
     7021 gtaagcaaaa cggacggatt cttgaagtta cgaaaaagga aaagaggttg catagtccac
     7081 gtttattttc gctatctagt ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca
     7141 aagctacact tgatgctgct caaggattgt acgaaagcaa gtttctaagc tatccaagaa
     7201 cggattcctt ttacattaca gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt
     7261 ataaaaaatt tttaggaata gaagaagtac agacaactga gatcaaagca aagaaacgat
     7321 atgtcgatgc gaaaaaagtt caagaacatc atgcaattat tccgacaaag acgattccga
     7381 ctcctgcacg ctttgcgaag ctttccaaat tacagcaagc aatctatttg caagttttgc
     7441 gcactactgt ggctatgttt gctgcagatt atatctacga agagacgacc gtaatgacgg
     7501 gcgtacagca attaaagcta aaaagcatcg gaaagatccc attacagcaa ggttggcagg
     7561 aaatcttacg catcaagtca aaaaagaaag aagctcagac tcttcctcct atcgtagaaa
     7621 atgaagaagc agaagtagaa ctgaaaagta tggagaaaga aacacaacct ccaaaaccct
     7681 ataccgaagg aacgttgatc acagcaatga aaactgcggg aaaaacgctg gataatgatg
     7741 aagcgcaaga aattctcaaa gaagtagaag gaatagggac agaagctaca cgagccaata
     7801 tcattgaaaa tcttaagcag aagcattaca tcgaagtcaa taaaaacgaa ataactgtta
     7861 cgccaaaagg aatcactctt tgtaaggcag tagccaatga accattactg acgagcgcag
     7921 aaatgactgc gcgctgggaa gggtacttga gaaaaattgg taaacaagag gggacaccat
     7981 ctgttttttt agagaatatc aaaaaattca tcctacattt attggcagaa gttcctaatc
     8041 aaatcgacaa ggtagacttt tcagatgaga ttagcgggac acgtcagata aaagcgatgg
     8101 agaagaaaaa cgatcaatta ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt
     8161 atcctaaaat cgctacatgc ttgaatccgg aatgcgactt caaattgtgg ccaacagtag
     8221 ctaaaaagaa gctgactaaa acgaatctca gagatttgct ttcaaaagga aagacaagta
     8281 aagttgtcaa aggttttact ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag
     8341 aagatatgac aatcggtttt tcttttccat ggatcgaaaa cagtggaaca aaagcagttg
     8401 atggaaacaa agaaacatca agaaaaaata atgaaaacga agtagagtaa atgaaaacaa
     8461 tgaaatagcg gaatatttat tccgaaaagt gagaatataa ataccaataa aactaaaaaa
     8521 tatcagtgaa tcaagcaaaa acgttggggt aggctgattt tatatagata gcaaggagtt
     8581 tttttatttg aaaaaagtgc attcattttt aaattatctt atgggagcaa tcacgattgt
     8641 ggctgtgtac caagcatttc taaagtatcc tgattacggg acgaagttgt tgtcattgac
     8701 gcacaatcaa acagcatatt caccaacgtt gattttatcg actttgactg caggagctaa
     8761 aagcgctact tatttttttc ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa
     8821 acaattgttg gtggtggtag cggctatgat cattatttat tttctaccag ttattacaca
     8881 agtatttcat caatattttg gttaagaata cgagagtttg tgacaaaagt catgtcacaa
     8941 acttttttct ttgtcatttt ttcgtaagaa aaagcaaaat aaaagtatat acattgatta
     9001 aaagtatata cttggatata atgtacttac tatagaatga gaatggtata gagggtggcg
     9061 aaatatgaaa aaaatgaaaa agtataaaat tagaaaatct ggcaatagcg atgtaacaac
     9121 tatcccacca gaagtaaaag aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt
     9181 ttttcaatct gatggttctg tacgaatgat caaagcacaa gaagaacctg atattgattc
     9241 attagttgat tcgatcatga atcaatatga agacgcgtta aaagatttgg ttgatttatg
     9301 aggtatcttt cgactaaaga aattatcaaa atcaacgcga aagttatttt acgtcactcg
     9361 ccaggcgaaa tgatcggtgt aaaagatgca aatgcattag atatggccgt caaacagcct
     9421 tcacaagcag tattcaatca agaactctat cctgaaatct atgaaaaagc ggcgatttta
     9481 gcaattaatt tagctaaaaa acatcctttt cataatggca ataaacgaac tgctcttgtt
     9541 gccatgttgt tgtttcttca attgaataat tgtccagttg ctttttctcg tcaagaagct
     9601 gttgatttta tcataatgat taccactagt tcattggatt tcgattcact aaagtccaag
     9661 ataaccagtt atttacgaca aaccgcaata aaatgaacga attaaaaact ttatttc
//
LOCUS       DAYYFD010000099         9603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000099 DAYYFD010000000
VERSION     DAYYFD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.100"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..271)
                     /locus_tag="ACHA25_002437"
     CDS             complement(<1..271)
                     /locus_tag="ACHA25_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8763689.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCG"
     gene            472..1686
                     /locus_tag="ACHA25_002438"
                     /pseudo
     CDS             472..1686
                     /locus_tag="ACHA25_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1865..2200)
                     /locus_tag="ACHA25_002439"
     CDS             complement(1865..2200)
                     /locus_tag="ACHA25_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8763690.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2184..2447)
                     /locus_tag="ACHA25_002440"
     CDS             complement(2184..2447)
                     /locus_tag="ACHA25_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8763691.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2907..3710)
                     /locus_tag="ACHA25_002441"
     CDS             complement(2907..3710)
                     /locus_tag="ACHA25_002441"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8763692.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3688..4812)
                     /locus_tag="ACHA25_002442"
     CDS             complement(3688..4812)
                     /locus_tag="ACHA25_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763693.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4828..5643)
                     /locus_tag="ACHA25_002443"
     CDS             complement(4828..5643)
                     /locus_tag="ACHA25_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8763694.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5633..6505)
                     /locus_tag="ACHA25_002444"
     CDS             complement(5633..6505)
                     /locus_tag="ACHA25_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8763695.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6590..7879)
                     /locus_tag="ACHA25_002445"
     CDS             complement(6590..7879)
                     /locus_tag="ACHA25_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8763696.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8023..8535)
                     /locus_tag="ACHA25_002446"
     CDS             complement(8023..8535)
                     /locus_tag="ACHA25_002446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763697.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8602..8751)
                     /locus_tag="ACHA25_002447"
     CDS             complement(8602..8751)
                     /locus_tag="ACHA25_002447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8763698.1"
                     /translation="MTMITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLI
                     LAHDR"
     gene            complement(9228..>9603)
                     /locus_tag="ACHA25_002448"
     CDS             complement(9228..>9603)
                     /locus_tag="ACHA25_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8763699.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2959 a         2037 c         1538 g         3069 t
ORIGIN      
        1 ttccacaatc atggcagaga aaacgggtac gttttaattc taaaatagtt aacttttctt
       61 tgactttcaa tatctgagtg cgtgtcgtat aggaaccgtt gcggataata cgaaccgatc
      121 gacagtgttc acaataggaa ggagagtcat ccaattttcc tttaataaac tgcgcttgaa
      181 cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta
      241 atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag gatgacgttt
      301 tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaagtgt
      361 tagtagaaaa tcgccttcga ttttccacca acacaaaata ttatagaagc aaaatcaatt
      421 ctaccttaga tgttcttata tcactctatc agtcctattt gacaaagagc caagaaaaag
      481 acgttggtcg tcgatgctgt tctctcgcct actcctcgtg cttgtagaag ttgtggttct
      541 actgtggtag atggaaacgg gaaagcaatt atagtgaaaa atgggaagaa ggaaacgatt
      601 gtccgttttg aacaatacaa tcatatgcct ttggttacgc gcctaaaaaa gcaacgctat
      661 acctgtaaaa actgtcgtac ccattggacg gctcaaagtt attttgtccg actcagccat
      721 tcaatcgcag atcatgttcg atataaaatt gcttctttac tgactgaaaa agtatctttg
      781 tcttttattg cgaaaagttg tcaggtatct ttgactaccg ttactcgtac attgaaagag
      841 tttaaaagct atttaccaaa gccttctaag aagattctcc ccagagtatt gatggttgat
      901 gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc agatggtgaa
      961 acaggaaact taattgctgt cttgcctacg cgtaaattac ctcgattaac aagctatttc
     1021 ttaggctgca ccaatccaga agaagtagaa ttcttagtca cagacatgaa cgccgcttac
     1081 ttccagctta ccaaacgtgt tctgccaaat gcgaaagtag tgattgatcg gtttcatatt
     1141 gtcaaacaca tgaatcaggc actcaatgag ttgcgtatcc gtgaaatgaa tgagcttcgt
     1201 aaagcaggac agaaaagtca ggcagaaaaa ctgaaaaaga actggcgctt tctgctaaaa
     1261 aatcgtgcaa gcatcaacca ttatgagtac aaaacatgga aaagtttccg agcaccaaaa
     1321 tacccatttc tcactgaagc aatgatgatt gatcgattgc ttgagttttc tgcgcctcta
     1381 aaggaggcgt atcccttttt tcatgaatta gtcgaagcct ttcgagacaa agaccctgac
     1441 ttatttttct ccttattggc agaacttccc gaaacgttag atgacggctt tcgggagaag
     1501 cttcaaaacc ttctgaccta tgaagaaggt atcaccagcg caatgatcta tccttattcc
     1561 aatggaaaaa ttgaagcgaa gaatacccac ataaagacaa tgaaacgagt atcctacgga
     1621 tttaaatcat tcgagaacat gagaattaga atctttttga tcaatcaatt aatcaatgta
     1681 agataacaaa aaatctgaac cagaatgtgg ttcattctga cccggatttg attttgcaca
     1741 actcatcagt ccttattgac aaagagccaa aaatctgagc cagaatgagg ttcattctga
     1801 cccagatttg attttgtaca acccatcagt ccttattgac aaagagccct tctttttagt
     1861 attattataa ttcttccatg cgttcttcca gtcgctgtaa atcgcttgga cttaattccc
     1921 caattttttt ccatttgacc tttttgttgt ttttgggtaa ttcgtttaga gttcctacgt
     1981 caatccaaga ttcttttttt agtcctgcct gtttccaatc ccttatttca acgtatttca
     2041 gctttatttt atcggattta gtatgatatt ttgaagtaat tgacagaaac gaaattgtgt
     2101 ccagtccatt ttttagaata tagacaggcc ggcgtttacc gccctctgca gctgtgaaag
     2161 caacgtagac ggttgctata tcattagtat tcatcttcat tgaaccattc ttcaattttt
     2221 tcgtcagtgt ctagtttttg gacaggcaat ttttccgcta atctttgaat attatggatt
     2281 cgtttacttt tttccgacaa ttcaatagaa aatgggattt ttccttgctc aacaatttgc
     2341 ttataaaaaa cggtgacggc tgttttgggg ctcatgccta gttcatcaat aatgctttct
     2401 gcttcatttt tcaatttttc gtccatagaa atttgtaact tagccatttt ctccacctct
     2461 ttctgacaaa cacattatat cacggaaaga gttcaaatgg aattaataca actacttttg
     2521 aactccttga aatgcagtgt atttatctaa atataaggaa aggatatagt gctaaatggc
     2581 cttgatagat agaaaagttt caaaatccgc cacagcgata attgaagcga acactcaacg
     2641 acacacagaa caggaaaaga ctcctaaaag aaccaaatta actaggacgt gtctgaaaac
     2701 tccacaaaga tgtggtatgc tctggtaaaa aacgaatggc acaaccacga agaccgatat
     2761 ggttcttgga aaactttctc cacccttttt gtaattggag ggatagtggt ttattgatag
     2821 cgattttcca agttcttcaa gtggaaccag acattgagca ccacggctaa aaagtgaatg
     2881 aagtctttat ttttaatcaa taatttttaa tgattaccag taattggttt tttcatgcgc
     2941 cctatttcta tttgatgtgg agttaaataa tgctgataag taaaaatttt tccgtcaatt
     3001 aaactgccac tagtgaatcc tgttctattg gtaaaaagag aatagtctgg gtattcatct
     3061 acaccaaatg ataaagattc agccataaac tgttttttac cagcaaattc agcactgcta
     3121 ccttgatgga tatgtcctac aaaaatacct aaaatatcac tttcggcaat caattcagca
     3181 aaaccttccg gaacttcagt tacaatagat ggatcttccc acgacaatgg atgatgaacc
     3241 aataaaatcg ttcccttttc agcaggtgta cccaaccact cttgtaattg aatcatctgt
     3301 cctatcgata tttttccttc gtgctgggta tcttgagcag aatccaagga aataattcgt
     3361 aacccatttt gattcgtaca atggatatac tctcccgttt cattgctttc aacactcatt
     3421 ccttcaaaga aaacactgcg acgatcatga ttcccacgac aaaaatagta aggtgtggtt
     3481 gggaaatatt gatccaacat tttccgtaag acttgataat cagaaatccc cccttcatgt
     3541 actaaatcac ctgttaatag aataaaatcg tacactgtta acgagagttc ttgtaacaat
     3601 ttttccaatg ccaactgtgg atttttaatt gttggaaata gttcagcagt aaacaagtta
     3661 gtttgatagt tttctctaaa atggatatca gagagatgca aaaagcgcat ggctattccc
     3721 tctttcttca ataaaataat caggatatcc taaattttct tgcgtctgat aatcaaaaaa
     3781 atgcattctt tcaggttcta tgcggagatt gactgcatca ccaatctgcc aagtaggttt
     3841 ttcattaagg acaatcattt ctataccatc gactttaacg gtgtacgcat aactttgacc
     3901 taatagctca cgatacttca cttgaccgtg taaatcgctt gtttcttttg aaaaggttac
     3961 gtgttctggt cttatgccta aatacaatga gccactaacc tgaagaagtt gtttttgcca
     4021 agctggattc aatgttaaaa attgactggc tacctttaat gttccttgat cataagtaac
     4081 tggcataata ttcatgcctg gcgaaccaat aaaatttgca gtaaaagtat ttttaggttt
     4141 cgtataaaca tttttcggcg tatcaatcat ctgaattcta ccttcattca ataaaacaat
     4201 ccggtcagct aacgtcatcg cttcaatttg atcatgagtg acgtagacaa aagtttgttt
     4261 ataacgttga tgaattttta ccaattcttt tcgtgcatcc aatcgtaaac gcacatccaa
     4321 atttgataac ggttcatcta ataaaaaata attactccgt ttaaccaccg cacgagccaa
     4381 tgctacacgc tgccgttgcc ctcctgacaa atctttagga agtcgatctt ggtatggcgt
     4441 taaacctaac atcttcaaag cttcttccaa acggtgctga atgtcagctg tcttaatttt
     4501 attcgccctt aatgcaaaaa taatattctc ctttacagtc atgtgaggat ataaagcata
     4561 attttgaaaa accattgcga tctctcgttc tccacaaggt agcttatttg ctagttgtgt
     4621 acctaaaaaa agctctccac tcgtaatttt ttccaaacca gcaatcattc tcagcaaagt
     4681 agattttcct gaaccagatg gtcccagtaa cacaattcgt tctccagcgg ccacagctaa
     4741 atcaatttct tttaaaatgg tatgttctcc atattttttt ccgatatttc ttaattcaag
     4801 cgtttctccc atgatttgtc ctccaattta tttaatacct gcagaaacaa aagtcccgat
     4861 aatatatttt tgaaaaattg catacaagac taacggtaca aacattgtaa ttaaactaat
     4921 cgccattgct acggtaaaat ttgtcccacc ttcagaacta atataggtct gcatcgccaa
     4981 cggcaaagta taattatcta ctgtccgtaa aattaatacc ggccaaacat actcattcca
     5041 agaggtaata aaaaaccaaa taccggtaga agtaatcgca tgtctagtaa gaggaaaaac
     5101 aattttactc atgacggttg tttgtttgat gttatccaat gccgccacat ctagaaggct
     5161 cactggaatt tcccgcattg tttgcgtcaa taaaaaaatg cccatcgcat cagcaaattg
     5221 tggtattatc acaccccaag tagtatcaat taaattaagt tttgcaatca acaaataatt
     5281 aggaatcatc gcaacagtaa aaggaatgaa catcgtacca atgatgataa agtaccagat
     5341 tttcttccct ttgattcggc aataagtaaa actatatgcc gcaaaaaaag ctacgcctaa
     5401 cttaacaacc gttataattg tagcaataaa aaaagtattt ctcgtaattt tgataattgg
     5461 aagattttcg aacaaatagc aaaaattttc caaggtaaac ggctgtggta tcaaactagt
     5521 aatcgtctga taggcatctt tcaatggctt aaaagaattg gccataataa acaataatgg
     5581 atagataata atgattatga tgaacataaa taagagatgt ggcaaccagc gtttaatttt
     5641 catagtaaac ccccttttcc acaaatttaa atattacccc taagaaaaca gcaaatatta
     5701 ataaagttaa aactgaaatt gctgaagatt ctccgacttt atacatttca aaagcctttt
     5761 gataagcata atagatcacg tttgcactac cattattcgg tccaccacga gtgatgatct
     5821 taatcggagt aaacacatac tgcagacctt gaacaatggt catcaccaat aaatatagtc
     5881 ctgtcgaact actcataggc aacacaattg taaaaaaaat gcgccagatg ggtacacgat
     5941 ccaacttagc tgcttcgatc atctcttgat caattcccac aattgctgca aaaatgacaa
     6001 tgaaattata accaaaaact ttccaagcag taatacctgc aatcacagca attacccaac
     6061 cttgtaaatt catccacata ggcagtctta aaccaaaatt tcccaacaag acagcaattg
     6121 gtcccgatac cggatttaat atccatgtaa aaaccatccc cgccactacc aaagaaatca
     6181 cagctggaat aaaaaataat ggtttataca aattctgccc tttttctata actattccta
     6241 ataaaaagga tgtaatatat gggaacacac agctcagcaa gacaaataga acggtatagg
     6301 acaaagtatt cttcaacact ttatgaaaga ctggatcagt cagcaccttt acatagttgg
     6361 caaagccaac aaattcttta gtgggggcca ccatattcca gtcaaaaaaa ctcaaataga
     6421 tcgtataaat caatggataa aaaacaaaca cgcccaacaa aataattgct ggcgccagat
     6481 acaaataacc tacatagcgc ttcatttccg acagcctcct catccttatg tcaaaagcag
     6541 acgaccttgt ttttcctttc ttacaggcgc tgtcgtctgc ttgctttgct tatttgagta
     6601 actcattaat ttccttttga gtagctttca tcgtttcatt gacggaatta cctgataaaa
     6661 ttttatcccg catatccaac aatttttgtt cagcttctaa tccagcatct ccaggaaatg
     6721 aactccaact gaccatctta tccatctgat caattgctgg tatcatcatc tgattttcag
     6781 ctaagaaagc ttttaatcca ttttgggcgt ctgcaacatc tttacgtggt ggtaaataac
     6841 cagtaccttc tgtccattta gctaaagatt caacactata aagatatttt aagaaatccc
     6901 aagttgcttc ctttttctta tccgtttgtg atgtaacagc taacattgcg ccaccagcag
     6961 gtagtttaac ttttttccct tcccaacttg gtgactcaac tgctttagca gtaaaagtac
     7021 tatttttttg aatgttgtcc ctttgagcaa tagtggtata gaccatcgcg acgttaccat
     7081 caataaagct ttgcacacct tgttcccacg tagtgtgtaa tgccgtctgg tcttttacca
     7141 tcctttgata taattcataa gcagcgatac catctttaga agcaaaggta gctttgccat
     7201 cttttattac acttgcccca ttactttcta aaattgcttg ttgtgcccaa ttatcagcag
     7261 gttcttgaat atacacgcca tactttccag ttttttcttt tattatctta gaaaaagttt
     7321 gaacttcttc ccaagttttt ggaccatttt catccaagcc cgctgctttt aatatatctt
     7381 gatttaaata taaaaccgga ttactaatgg agtaaggaat acccacttgt ttaccatctt
     7441 tttcagacag tgccatgata ttgtctaaaa aatgatcttt aaaataatca gcattatccg
     7501 gatccacttc ttctactaaa gattgaggat tagtatattt gaaattttct gcaaaataat
     7561 ctttaaaagc ccatcccact tgtactacgt ccggtatttc tttggaggca acagaagctt
     7621 gtaaattttg catcaagcct ttatacatat caggattata ttttgctaca accttcactt
     7681 tgtcttgaga ttgattgtat ttatctacta attcattcac tgtttttcca cctagggtat
     7741 ccgcattaac atgccaatac tctatcgtta cacgaccctc cgattttttt gttgtcttgg
     7801 tattcccact accacaagct gccaaagaga tgacacttaa aacagacaca acccctataa
     7861 caagattttt ctttaacata acattaactt cctcctaatt tctttatggc catttttcta
     7921 atcaagaaat attatgaact accaatgtaa atttctagtt aattttctgt aaataattcg
     7981 aaatttgtgc atttcggggt acagggtcac tggacatatc catcaagcta agaataaagc
     8041 ccccatatgg tcatgtatcc catctccaaa tctcttaact cctctgtttt gattataaaa
     8101 tagtttattc tgtgctcctt tcttccataa ataggcccat gcacaacgtg aggttatatt
     8161 ataaacacca cagcgcgcaa ggtactcaat cgtttctagt tcattgactg tccatacaga
     8221 aaatgtcaat cctagctgct gccccacttc acgggccaac ttagttaacc atccttcatg
     8281 aatattgacg atttgaacgc tttgataggc taatttctgt aaggtatcga tcaaatctgc
     8341 ttcggtaata tccccaagtt ttttcccttc gaactgattt tcaatattat aaaatatatg
     8401 ctttccccaa atcgcagccc aagataattg tacatagcca ctaagaacaa tttgtggcca
     8461 gatttgatag gtttgtgcca gcgaaaaaac ggccatttcc aatccctctt cttttaaatc
     8521 acagttaatg gtcatggttg ccttctcatt ggccactgct tgaaacacct gctcaagggt
     8581 taccgaatct gggacaactt cttacctgtc atgggctagt attagcgtac catctgtttg
     8641 ctgacgcaca tctacttcaa aacaaagctg gcgatccaaa caaaagcgaa gaaatgccca
     8701 gctattctcc tccgtattgt cactaccaga gtgtgcagtg atcatggtca ttttgacccc
     8761 tcctttaatt ccgtgatttt ctcccacacg tccactatac cgatgaagcg ttaatttctt
     8821 gttaatttct tgtaaattcc ctgcgttttt tcagtaaact agttgtaaat cttgcatgct
     8881 ccttcctacg aactcatgaa tcgtcgattt tgcccttcta gctagtttca ccaattaagt
     8941 agttctacat tccccgcttt taacctacta cacctttttt tgttttcagc tattccttct
     9001 tcttgctgaa agtccttgag gttctttctt acaggatacc atcaaggtgt tcaatggctt
     9061 attcccattg aatgaccgag acttactatc agatttctgc tcgttgaacc aagtctttga
     9121 tacaggattt cttccgtcat cttctcacaa ataccacctt gcgtttctct agcgattggt
     9181 cgatgtgcac cctcccagtg gactgtcact accccttttt gctatgccta gcccacaaaa
     9241 aaagctgtcc tcacagacag ccagactgta gacaaaagct gttccaaact cgtaactcga
     9301 gcttggaaca gctttttatc tacttcgttt cgaatttttg aacaaaaaaa taaaggctcc
     9361 ttgcctttcc atagccaggt ggctaatttc tttaaattca ttgcagcaaa cactagcatc
     9421 gtatgcgtcg cgactttttc caagcctcga tacttggtcc aacgcatccc atgcttttct
     9481 tttgcatccg caaatattcg ttcaatcgtt tgtttccgtt ttttgtaaat tgatttattc
     9541 agatctgtta ggcgaagatg ttccacttca tccatcagat ctccccaaac atgtctcgtg
     9601 atg
//
LOCUS       DAYYFD010000100         9546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000100 DAYYFD010000000
VERSION     DAYYFD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.101"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..977
                     /gene="gnd"
                     /locus_tag="ACHA25_002449"
     CDS             75..977
                     /gene="gnd"
                     /locus_tag="ACHA25_002449"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8763700.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            979..1980
                     /locus_tag="ACHA25_002450"
     CDS             979..1980
                     /locus_tag="ACHA25_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8763701.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1977..2261
                     /locus_tag="ACHA25_002451"
     CDS             1977..2261
                     /locus_tag="ACHA25_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8763702.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2294..3091
                     /locus_tag="ACHA25_002452"
     CDS             2294..3091
                     /locus_tag="ACHA25_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8763703.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3105..3938
                     /locus_tag="ACHA25_002453"
     CDS             3105..3938
                     /locus_tag="ACHA25_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8763704.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3956..4813
                     /locus_tag="ACHA25_002454"
     CDS             3956..4813
                     /locus_tag="ACHA25_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8763705.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4835..5344
                     /locus_tag="ACHA25_002455"
     CDS             4835..5344
                     /locus_tag="ACHA25_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8763706.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5381..5782
                     /locus_tag="ACHA25_002456"
     CDS             5381..5782
                     /locus_tag="ACHA25_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763707.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5894..6247
                     /locus_tag="ACHA25_002457"
                     /pseudo
     CDS             5894..6247
                     /locus_tag="ACHA25_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6281..6724
                     /locus_tag="ACHA25_002458"
     CDS             6281..6724
                     /locus_tag="ACHA25_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8763708.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6741..7826
                     /gene="uxuA"
                     /locus_tag="ACHA25_002459"
     CDS             6741..7826
                     /gene="uxuA"
                     /locus_tag="ACHA25_002459"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8763709.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7819..8532
                     /locus_tag="ACHA25_002460"
     CDS             7819..8532
                     /locus_tag="ACHA25_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8763710.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8739..8888)
                     /locus_tag="ACHA25_002461"
     CDS             complement(8739..8888)
                     /locus_tag="ACHA25_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763711.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9284..9484
                     /locus_tag="ACHA25_002462"
     CDS             9284..9484
                     /locus_tag="ACHA25_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8763712.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3211 a         1498 c         2113 g         2724 t
ORIGIN      
        1 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
       61 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
      121 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
      181 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
      241 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
      301 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
      361 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
      421 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
      481 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
      541 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
      601 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
      661 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
      721 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
      781 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
      841 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
      901 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
      961 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
     1021 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
     1081 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
     1141 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
     1201 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
     1261 caccagacaa tcgtatctat catacctgct ttaattgaac gaaatgctgt agcagaaaat
     1321 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
     1381 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
     1441 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
     1501 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
     1561 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
     1621 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
     1681 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
     1741 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
     1801 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
     1861 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
     1921 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
     1981 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
     2041 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
     2101 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
     2161 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
     2221 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
     2281 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
     2341 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
     2401 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
     2461 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
     2521 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
     2581 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
     2641 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
     2701 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc
     2761 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
     2821 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
     2881 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
     2941 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
     3001 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
     3061 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
     3121 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
     3181 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
     3241 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
     3301 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
     3361 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
     3421 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
     3481 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
     3541 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
     3601 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
     3661 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
     3721 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
     3781 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
     3841 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
     3901 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
     3961 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
     4021 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
     4081 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
     4141 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
     4201 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
     4261 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
     4321 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
     4381 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
     4441 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
     4501 ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc
     4561 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
     4621 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
     4681 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
     4741 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
     4801 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
     4861 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
     4921 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
     4981 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
     5041 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
     5101 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
     5161 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
     5221 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
     5281 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
     5341 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
     5401 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
     5461 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
     5521 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
     5581 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
     5641 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
     5701 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
     5761 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
     5821 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
     5881 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
     5941 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
     6001 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
     6061 gggaaattat ttttcaaata aagaacagtg actgaaacaa gttgataagt tgaaagaaaa
     6121 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
     6181 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
     6241 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
     6301 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
     6361 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
     6421 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
     6481 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
     6541 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
     6601 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
     6661 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
     6721 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
     6781 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
     6841 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
     6901 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
     6961 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
     7021 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
     7081 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
     7141 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
     7201 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
     7261 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
     7321 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
     7381 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
     7441 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
     7501 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
     7561 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
     7621 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
     7681 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
     7741 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
     7801 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
     7861 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
     7921 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
     7981 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
     8041 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
     8101 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
     8161 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
     8221 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
     8281 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
     8341 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
     8401 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
     8461 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
     8521 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
     8581 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
     8641 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
     8701 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
     8761 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
     8821 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
     8881 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
     8941 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
     9001 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
     9061 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
     9121 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
     9181 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
     9241 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
     9301 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
     9361 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
     9421 agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc
     9481 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa
     9541 aaattc
//
LOCUS       DAYYFD010000101         9495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000101 DAYYFD010000000
VERSION     DAYYFD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.103"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1108
                     /locus_tag="ACHA25_002463"
     CDS             134..1108
                     /locus_tag="ACHA25_002463"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8763713.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="ACHA25_002464"
     CDS             1090..1551
                     /locus_tag="ACHA25_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8763714.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="ACHA25_002465"
     CDS             1601..2065
                     /locus_tag="ACHA25_002465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8763715.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="ACHA25_002466"
     CDS             2079..2828
                     /locus_tag="ACHA25_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8763716.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="ACHA25_002467"
     CDS             2828..3658
                     /locus_tag="ACHA25_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8763717.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="ACHA25_002468"
     CDS             3658..4305
                     /locus_tag="ACHA25_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8763718.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4403..5179)
                     /locus_tag="ACHA25_002469"
     CDS             complement(4403..5179)
                     /locus_tag="ACHA25_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8763719.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5323..6282
                     /locus_tag="ACHA25_002470"
     CDS             5323..6282
                     /locus_tag="ACHA25_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8763720.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6399..6707
                     /locus_tag="ACHA25_002471"
     CDS             6399..6707
                     /locus_tag="ACHA25_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8763721.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6734..7033
                     /locus_tag="ACHA25_002472"
     CDS             6734..7033
                     /locus_tag="ACHA25_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8763722.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7207..7758
                     /locus_tag="ACHA25_002473"
     CDS             7207..7758
                     /locus_tag="ACHA25_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8763723.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7904..8197
                     /locus_tag="ACHA25_002474"
     CDS             7904..8197
                     /locus_tag="ACHA25_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8763724.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8190..8486
                     /locus_tag="ACHA25_002475"
     CDS             8190..8486
                     /locus_tag="ACHA25_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763725.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8557..>9495
                     /locus_tag="ACHA25_002476"
     CDS             8557..>9495
                     /locus_tag="ACHA25_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8763726.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFR"
BASE COUNT         3356 a         1363 c         1780 g         2996 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa atttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtattgtaaa gttatatgac tttataatac ttattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtatcaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgacttc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     4321 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     4381 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     4441 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     4501 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     4561 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     4621 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     4681 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     4741 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     4801 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     4861 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     4921 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     4981 cccttaatcg ttcaactgtt ccactttctg ctaaaagttt aaaagctttt cgaatggtaa
     5041 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     5101 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaaa tataatggtc
     5161 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     5221 tattaaaacg tcgattgtaa aattaagcta gacaactaaa aagatcattt gtgctacact
     5281 caaaatagtt ccgaccaaag aattaataag gagtgagtac aaatgactta cacccatctt
     5341 acaacagacg aactagtaat catagagtct tatttcaata tcgagaaacc tgtcgctctt
     5401 gtcgcacaaa gtttaaatcg ctctagacaa accatttata atgtttattc gtttctcaaa
     5461 caaggaaaaa aagtgctaga ttattaccgt caatacaaac aaaataaatc tcgttgtgga
     5521 cgacgttcaa ccgttttatc tgatgaacaa tcaacatatg ttcaagaaaa ggtagctcaa
     5581 ggatggtcac ccgatgtgat tatcggccgt ggcgagtttc caattgattg ttctgttcgt
     5641 acactctatc ggatgtttaa aaaaggcctt tttgacacac agtcgttacc tatgaagggg
     5701 aaacgtaaac cgaataatca tcaagaaaag cgtggaaaac aggcctttcg cagaacgatt
     5761 catgaacgga acagtctcta tccacacctg actaaagaat ttggtcatct cgaagtcgac
     5821 accattgtgg gagttaaaca caagagcgct gttattacat tggttgagtg tttatccaag
     5881 gttattatta ctttgaaacc gacaggcaga cgagccgaag atatcgaaaa caggttaaat
     5941 gattggttca aatccatgcc taaacacttg tttaaatcca tgacatttga ttgtggaaaa
     6001 gaattttcca actggcgatc tataagtaac tcgaacgata ttgccatctt tttcgcagat
     6061 ccaggaacac cttcgcaaag agggctaaat gagcactcaa acggcttgct tagacgagat
     6121 ggcttaccta aaacaatgga ctttagggac acggatgaaa cctttattca atccgttgcc
     6181 tcgaaaagaa accatattcc aagaaaatca ttaaattatc gaacaccatt ggaagtattt
     6241 ttgagctgca tagataaaga catattgtct agcttaattt gacaaatcag atagtataaa
     6301 atgaatcaaa ttgaatatca aatatatatt atatccatga agcccaatct tctttaatag
     6361 aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat aaaggacaac
     6421 gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct ttatgttgga
     6481 atctaatcga tcaaaacgtt caaaagcaat ttcatttaga ctatcttcaa atctttgaat
     6541 tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaacca gaagcaagaa
     6601 aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa atatgggtca
     6661 ttgattcggg tgagtatcaa acgatgttat taccagaaga atactgatat ctaaaagaaa
     6721 ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg cacagctagt
     6781 cactactcct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg aaattgaaat
     6841 gggtatgctt cctgaagatg ttactctacc acgtttagtg atgacacatc tttatattta
     6901 ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata ttaccagcca
     6961 gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc agattggagg
     7021 aacgaatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa gatatcctta
     7081 cagcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt ttcaactagt
     7141 attgacactt ataacggcaa tggtgattga attgcttgaa caaaaattag cataggagaa
     7201 aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg agatcctcat
     7261 catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga agcggaagat
     7321 aaattagcgg tagtcattac tattcgtcca acagatacag aaccatttac gaatgatttg
     7381 agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt tattgctgtg
     7441 aatctttttt cgtatacaaa agcgaaaagt cctcgggtat ttcctcgtgg aaatgatgaa
     7501 caatctttgg aaatattaac aactgttttc acagaaaaga aaatcaacac cattattttt
     7561 gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcgaa gaccatttac
     7621 gatcaattga gtgcgaaaca aaaaaagatg accaaagtat tgataaatgc agaaggtaag
     7681 ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga tagtcaatta
     7741 ttatttcaag aaaaataaca ttttattttc ttatgttact tataagtagt tttacatgct
     7801 ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat gtacccagta
     7861 atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga ttgatgaaac
     7921 ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg aactggattc
     7981 agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt ttccaatgaa
     8041 aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac aggaaaaaac
     8101 tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga ttcaaaaagt
     8161 aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta ggtggtttaa
     8221 tttacggtgg atattggtta tataaatata tgcttgcgca tccgaatata gataaaggaa
     8281 aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact aagaaaaata
     8341 atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga agggaaattt
     8401 ctactagttt taatccatct gaaagccctc aaggaagaga tcgttgtcat gctggctatc
     8461 gacattcttg ggaaaggatt aactaaatga acaccattga gattaattga ctgataaaat
     8521 aagtacgcga gggagaaatt gaaaggggga aataaaatga aaaaatggtt gttattacta
     8581 attggaatgg ggattttatt cactggttgt acatctgaga atacgaggac taaagagact
     8641 gtagaaactg atacaacaat tgaggctaaa aaaagtaaaa cgcaatcaac tgaacaaact
     8701 acaacagaaa tgaaacaatc tgtggaagca agtacaagtg aaagttcaat cattgaatct
     8761 tcttctgaag aggttaaaaa tactttgtgg gatagtgaga aatctactca actagcaatt
     8821 tttgtcccac aatggggaaa gacattaggt caagaatata aaagctacaa tcctcagaac
     8881 aatgtaagtc tgtatggtac gcctttacca agtgcagtta ttgatggaaa ttggaaaatg
     8941 gcaatcaatg aagcaccagt taccgtacaa tggtcagaag atggaactgg tcatgcagat
     9001 ttcaatttag tagcagttta ttctgatgta gaaactggag aatatttagg gcagcatgta
     9061 tatttctttg gctttcaaaa tggacaacct aaagtttatt taacgcaaca aaatcaaggc
     9121 aatgataaaa actacatgta cttcaatgaa actcagaacc aacagttaaa acaaggtttc
     9181 attgatattg tgaatggaca aactcctcaa actccagtag tagaagaatc tactcaacaa
     9241 gcaacaacta caacgacaca atctacaagt agctcagcaa tttcaagttg ggaagctgct
     9301 aaagaaactg tactaaataa taaagcttta tggttgtatg attctcctga taaaaatgca
     9361 tcaaatacta atttaattag cacaaatgaa tatatgaaag atctaatgca agatgaaaat
     9421 ggaagctact attcaattaa tattgtaacg gatgataatg gaaatacaac aatggctggt
     9481 ggaaaacaat tccgt
//
LOCUS       DAYYFD010000102         9306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000102 DAYYFD010000000
VERSION     DAYYFD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.104"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..705)
                     /locus_tag="ACHA25_002477"
     CDS             complement(37..705)
                     /locus_tag="ACHA25_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8763727.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            839..1126
                     /locus_tag="ACHA25_002478"
     CDS             839..1126
                     /locus_tag="ACHA25_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763728.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1542..3080
                     /locus_tag="ACHA25_002479"
     CDS             1542..3080
                     /locus_tag="ACHA25_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8763729.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3077..4042
                     /locus_tag="ACHA25_002480"
     CDS             3077..4042
                     /locus_tag="ACHA25_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763730.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4029..5351
                     /locus_tag="ACHA25_002481"
     CDS             4029..5351
                     /locus_tag="ACHA25_002481"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8763731.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5414..5518
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5592..6335
                     /locus_tag="ACHA25_002482"
     CDS             5592..6335
                     /locus_tag="ACHA25_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8763732.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6350..7342
                     /locus_tag="ACHA25_002483"
     CDS             6350..7342
                     /locus_tag="ACHA25_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8763733.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7354..8091
                     /locus_tag="ACHA25_002484"
     CDS             7354..8091
                     /locus_tag="ACHA25_002484"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763734.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8081..8803
                     /locus_tag="ACHA25_002485"
     CDS             8081..8803
                     /locus_tag="ACHA25_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8763735.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8887..>9306)
                     /locus_tag="ACHA25_002486"
     CDS             complement(8887..>9306)
                     /locus_tag="ACHA25_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8763736.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAVTPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3049 a         1604 c         1965 g         2688 t
ORIGIN      
        1 tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact gttcaagagg
       61 atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc
      121 aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt
      181 atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat
      241 caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat
      301 atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag
      361 aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct
      421 ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc
      481 gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt
      541 gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc
      601 atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc
      661 taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc
      721 ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa
      781 aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat
      841 gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg
      901 agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc
      961 aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt
     1021 tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat
     1081 tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa
     1141 acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg
     1201 atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac
     1261 tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag atatagctga
     1321 ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa cgagaaccaa
     1381 aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta gaggtatttt
     1441 tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa
     1501 agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat tttatacaaa
     1561 cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg tccatttctc
     1621 tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg gcagccaatc
     1681 ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg attccttcac
     1741 ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca gcattgattg
     1801 cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt ctagctgaaa
     1861 ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg agacgcttgc
     1921 tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga actgccttag
     1981 ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga ttagggacat
     2041 ttattttgtt aggtattgac cgaaatacac ctgttttgac attgattggt gcgatcagtt
     2101 ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat aagaaacctc
     2161 gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc
     2221 agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga gcggaacctg
     2281 aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca gaagttgaat
     2341 taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa ggtcaaatcg
     2401 atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa
     2461 tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa ttattgaata
     2521 agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat gccttggcag
     2581 tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg gcagctatct
     2641 ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat ggatataaag
     2701 gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag ccaagtttgc
     2761 gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc acagatagcc
     2821 agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc ccagcttatc
     2881 aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc gttaaagcac
     2941 tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat taccaagtca
     3001 atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat gaaggattag
     3061 taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga
     3121 tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt
     3181 ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg gattatctaa
     3241 accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata atctgcagaa
     3301 aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga ccatgacagt
     3361 caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga aaagagcaga
     3421 ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc gagatcgtat
     3481 gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag ctattgctgc
     3541 aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga tttcaagaaa
     3601 ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca tcgttttcgt
     3661 tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta tgaaggatgg
     3721 agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa acgactacgt
     3781 tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag atttagtcgt
     3841 caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt gctttgatac
     3901 gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga tagtagaaaa
     3961 gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa gtcgagagga
     4021 ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg
     4081 ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg
     4141 gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg
     4201 cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca actgccaact
     4261 gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa agccgaaaaa
     4321 aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt ttggatgatt
     4381 catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg gcaaccgggc
     4441 aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt gcagactttt
     4501 tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat attgcttttg
     4561 agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat cataatcaac
     4621 ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa atggaagcat
     4681 ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa ggtactcgct
     4741 atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt gcgactggga
     4801 gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt gataaacacg
     4861 gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg tgtggagata
     4921 ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga aattatgtag
     4981 ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata cctacgatcg
     5041 tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag
     5101 atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag
     5161 ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct gcagtgatta
     5221 gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa aaattgacta
     5281 aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat ctctcctatt
     5341 tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct tgtgctacac
     5401 tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt aagttgtgaa
     5461 cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag cagacgcttg
     5521 aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat tagaagaaag
     5581 gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc attttaatta
     5641 tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc ggacctgctg
     5701 ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct gtcacgacat
     5761 taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt actgccttgg
     5821 caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg atttcacaaa
     5881 tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc ggtatgcttc
     5941 caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct ttttcggata
     6001 cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg gcagatacag
     6061 ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt tccggtttaa
     6121 aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta agcgctcaag
     6181 caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat aaagtttttg
     6241 ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt tcattattga
     6301 aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt
     6361 atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa aagaaacaag
     6421 tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt atgtaccgaa
     6481 tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag aggctggttt
     6541 aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag taggtgcgga
     6601 caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg cagatggaca
     6661 gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg gattcgccac
     6721 cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat atgcaggttg
     6781 gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt
     6841 ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag gaaaatcctc
     6901 agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag cgggtattga
     6961 actaaactat atcccattga aagaattaga tgaacggtta gattactata ctccggtaat
     7021 tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt ttatggatgc
     7081 cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta aactgttgca
     7141 aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa
     7201 aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt ggcgcaatta
     7261 tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc aagaactatt
     7321 cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag ccacttgaat
     7381 gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc taaagatacc
     7441 tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa aactttaaca
     7501 ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt aactggtctc
     7561 catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct gagcgtctat
     7621 caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga acaagtaagg
     7681 gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga acaattatct
     7741 ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc ttcttacttg
     7801 cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat gcaagactgg
     7861 atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca tgatatcgaa
     7921 gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc acatgtgatt
     7981 gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca gcaatcagag
     8041 ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg atgctcattg
     8101 tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga ttttgacgat
     8161 catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag caagtaagac
     8221 tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt ttgaacaagt
     8281 tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata atatttggac
     8341 gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt ttgcagccat
     8401 aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct taaattatat
     8461 ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact atcaacaaga
     8521 ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga atccagcagt
     8581 ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg atggactaca
     8641 tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct cactgcttga
     8701 agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa
     8761 tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag atgtgacaca
     8821 actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt gattaataaa
     8881 tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata
     8941 acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt
     9001 taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga
     9061 accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg ctggagtaac
     9121 agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtttttc
     9181 cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt cttgaacggc
     9241 tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta attgttgtcc
     9301 tgagta
//
LOCUS       DAYYFD010000103         9273 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000103 DAYYFD010000000
VERSION     DAYYFD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.105"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1216..2382)
                     /locus_tag="ACHA25_002487"
     CDS             complement(1216..2382)
                     /locus_tag="ACHA25_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8763737.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            complement(2470..2898)
                     /locus_tag="ACHA25_002488"
     CDS             complement(2470..2898)
                     /locus_tag="ACHA25_002488"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFM8763738.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            complement(2984..4408)
                     /locus_tag="ACHA25_002489"
     CDS             complement(2984..4408)
                     /locus_tag="ACHA25_002489"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8763739.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            complement(4776..5786)
                     /locus_tag="ACHA25_002490"
     CDS             complement(4776..5786)
                     /locus_tag="ACHA25_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFM8763740.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            complement(6038..6976)
                     /locus_tag="ACHA25_002491"
     CDS             complement(6038..6976)
                     /locus_tag="ACHA25_002491"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM8763741.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            complement(7013..8104)
                     /locus_tag="ACHA25_002492"
     CDS             complement(7013..8104)
                     /locus_tag="ACHA25_002492"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM8763742.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSINMLASTS
                     IVFLLISNNFIRIIQNIFVLNYIIYASVLMSNYKRTKVKNYIYLLAYFFFIIVCLIFI
                     NIRGTAFVNGILPLFRENIIFKAVYYFFE"
     gene            complement(8076..8972)
                     /locus_tag="ACHA25_002493"
     CDS             complement(8076..8972)
                     /locus_tag="ACHA25_002493"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8763743.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(8962..>9273)
                     /locus_tag="ACHA25_002494"
     CDS             complement(8962..>9273)
                     /locus_tag="ACHA25_002494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763744.1"
                     /translation="KCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVK
                     RYNVGINYNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDE
                     K"
BASE COUNT         3188 a         1667 c         1175 g         3243 t
ORIGIN      
        1 cctcttctgt ccaaattatt ctaagatttc tctttaccaa catcatttga caaagagctt
       61 cttttttgaa tactatattc gttctgtttt tcatcttcaa atacgaactc atccgcagaa
      121 atatttgtca caactccatg aatcttcttt tcattaccac ctatatacca caggattcct
      181 acacagaaac aacaaaaaaa tagcataaaa taggttttct ttattttata tccctccttt
      241 tagattaaaa gctcacagaa aacgctttct aaaaagacaa tagaataatc ataatttttc
      301 attttgtatt atttatacaa ataatacgca tgttactgaa aaatgatatg gttccctact
      361 ttttgagata agttaaataa aatctgtcat cgataaaaca gctagtacag taatgataaa
      421 tagtaagaac ttaaaatatt ttggattttt atttttgaaa taatcgaaga aaagtaaaag
      481 tgataacgaa atagcaaaca agattatatc tataagatta ttcattgaga aatcgcccca
      541 tatggaactc tattttgacc tgacacaccg agacataaag caatcatatc ctctgcattg
      601 ttttatacta ttagaactat agctacacct attggatgct actggctcta agattaatgc
      661 taatacagta acatcaatac ctaaactagc agaaactcta tttgcatctt caatttcaga
      721 tgcctggact gtcgtgacta gcgccgtagt agaaaaattt actggtacta acaaagatgc
      781 agacacgact tttatttcac ttatccttta tcccataacc taaacttcaa tcattacttg
      841 tgagctgatc aatgttcttc caagtctgaa agaacattga tcagcttggc aaacaaataa
      901 tgttttcaaa tacttgatcg atgaaaaaaa gctattggta ataacagtaa ttgcattttc
      961 cattttcgtg tcagatactt cgcttacagc tggtaaacga tcaatcccat tgtcaaattt
     1021 tggatttttt tctgatgatg tcttgcttta gttcataaaa taatccagca ataaattcct
     1081 cgcaaggata gccaacttca ttttcctaat cggaattttt aaacttatcg gtatagatac
     1141 agatgcaatg agaacaaaca tttatttcga gtcaccatct aaaatatttt cctccatgta
     1201 cccgttcatt attatttagt tatcaccaaa gatatctctg gtataaactt tttcttcaac
     1261 atcctttaaa atatcttcaa accgattaga tacgatcaca tcagaaatat ttttaaattc
     1321 attaatatct ttcaatactt gtgaatgata aaattcatct tcatccaatg taggctcgta
     1381 aacaaccact tcaattcctt tggctttaat ccgtttcata attccttgga tcgacgaata
     1441 tcgaaaatta tctgaaccgg ccttcatcgt taaacgatag attccaacaa catttggttt
     1501 tttcgccaaa atctgtccag caataaaatc tttccttgtt gtatttgcat caacaattgc
     1561 actgatgata ttgcttggta ctttttcata gttggctctt aattgttttg tgtctttggg
     1621 taaacagtat ccaccatagc caaaagaagg attattataa tgcgaaccga tccgtggatc
     1681 caaaccgaca ccttcaataa tttgcttact atcaagccca cgaaattctg catacgtatc
     1741 taattcattg aaatacgcca ctcgcaatgc caaatatgta ttcgagaata atttaatagc
     1801 ttctgcttca gttgagtgag tcaaaagaac tgatgcatct ttgttaaatg cgtttctttg
     1861 aaacatagtt gcaatctcgc gaccacgagc agaacattct ccaactataa ttcttgatgg
     1921 atataagttg tcgtacaaag cttttccttc acgcaaaaat tcaggtgaaa aaatgatgtt
     1981 ttctgtacca aatttcgctt taatttcatt tgcatagcca actggaactg tcgacttaat
     2041 aatgaatgtt gaatctggac gaattgctaa agccttctca attacatctt ctactgttga
     2101 tgtattaaaa tagtttttct tttcgtcata atctgttgga gttgcaataa tcaagtaatc
     2161 accgaagttt actgcatcag taaaatttga cgtaaattcg acatttaagt catcccgttt
     2221 taaaaattca tgaacttctt tatcttctat tggtgagatg ccattttgta acatcgtgat
     2281 tttttcttcc acgatatcat aagctttgac ctgctctttt tgtgctaata atagtgaatt
     2341 tgctagacca acatagccta atccgaaaac tgaaattttc atattcttcc atacgctcca
     2401 atcaaatttc attttatagt attttagctg ttatcctttt gaacatattc cttattcaat
     2461 caatattatt taatagaaaa aatttttttg cttcaaaatc atcgaggttc gatatttaat
     2521 aaattcattt ttatttttct cataatgctc ctctatagtt gaaatcactt tacatgaact
     2581 acccgctaca acactattat ttgggatatc tttatttact aaagtgttcg ctctaataat
     2641 tacattatcg ccaattgaaa ctcctgggaa aacaatagag taaataccaa taaaaatatt
     2701 atttcccaat ttgatcctac ctatttttga agttgactcg ctcacagaac tcatcttcct
     2761 taagacccag actccaccat cgtgtgttat aaatttaaca ccaaatgaac atctgaaatt
     2821 atcccctatt tcaattaaat atggctcgct cccaaattca ggaacctcta ctaaactatg
     2881 tttttttccg aatatcaaac cttcattaat acattctcgc agattttttc caaatagttt
     2941 tctctttatt cttcgaaaga ttctattctt acttcgtata ccctcacctt ttagccattt
     3001 ttattctgaa aaattttttt agatacgtta taattaattg tttatacgta aaaacgaaaa
     3061 tagcactaaa aataatcgtt aagaaaagat taaaatagaa gcctctatga atgcatacaa
     3121 aagcgattgc ggaatagaga actaaaagta gcgatattcg cttccaatca aatgcataat
     3181 aagcaatttt ccttccaaaa taatatctaa gtccaaataa tactaaagta ccgagcaaag
     3241 tagcaaatga gacaccaatt actccaaaag aatagcctaa taaataggtg ccaacaacac
     3301 tcaatagacc tgacactact gaaatcatta ataactgatt tgttttattg tatactgcaa
     3361 atatatttcc gatatttgtt gtcatcccac tgaaacatgc cataataact aatattggaa
     3421 ctagaactaa tgatttttta taatcagggt tggttataaa agagtatgca cagcaaatga
     3481 taggaataga aagagtcgct aacctaatgt acattttaaa aatataggta tttttctcaa
     3541 cgaatctttc gatgaaatca tcattctcag acgtgataac cgcatcctct atcatagaca
     3601 tactaacaat acttgtaaga gttaaaatta tagatgaaaa tttcatcgca aaagaaaaaa
     3661 taccattttc tgttactccg atgtacgttc caatataaaa tcttgaaaaa ccaactatta
     3721 gccatccaaa agtgagattc aaagtgagtg ggatagagta tttcagcatg gactttaaaa
     3781 ataaaaaatc aaaagaactc gttgcgaaat attgaaagat ctgccccctg aaaatcaaaa
     3841 aaatcaaatt tataacatta gaaattatga tagagagtaa gagaccaagt atccccatac
     3901 ccattgtaac aacaaaaata gtaaggagta acaggttgat acccgcagcg attataccac
     3961 tcatcacaaa aatctttgat tcacctagtg ctcttacaat atattgaaaa acacttagga
     4021 taggagtgaa aattaattga aaaacaaaca attgataata ctctaaattc gggaaaatca
     4081 ttctatatat tattggtaaa aaaatggcta atatcactgt atttgctata acaaaacaaa
     4141 tactgttcgt cactactttt ttctgaatat ttttttcttt gcctaatccc atgcgtaaaa
     4201 ttgcttccca tattgctcca aaaataactg gagaaagaaa ttgagcaatt gtattttgat
     4261 aatcaaagct tcctaaatcg gcaacagaaa tatatactgc atagataggg attataatga
     4321 cagatatcat ttttgatgat aatgttccta ttgcgtaaat aaaagctttt ttagctattg
     4381 aatgtgattt attactgcta ctatccaacc gtatgacctc cctcaactca catctataga
     4441 tgactttctg gtttaattag aactacctaa attcctctgt atcgtgtgag tcctcgtagt
     4501 aaaaattttt ttgaatatgt gaatagctaa gatcttttca aataaagata tccatgatca
     4561 actatatatt tcttcaatct catcaatcaa tattggcagt cagaatccag ttcacacatc
     4621 ttgatacaca attttgtttt atagaaattc ttttagttat agaaaatatc tattcggcga
     4681 gaaagttcgt cacgtttgaa agtagaattt cctaaaatac ctaacaaagc taaaaaaatc
     4741 gtatgaatta cccatcttca atattccttt caaaattaac tcaaataata cactgtatac
     4801 cattcaacga attttttaat gccctcatcc aaggttatat tcggtttaaa gttgattttt
     4861 tcctctaaat cttcaatatc ggcataagtt ctaacgacat cacccggctg catgtccaaa
     4921 aaaatcttgt tcgctttaac acctaaatac ttttcaattg tttcaacaaa ctccaacaat
     4981 gcaaccggct tattatttcc aatattgtat atcttatatg gtgcccaact ttcactactc
     5041 aaatcatcga tgtctgtcca gtcggagttg ggttcaggta ctttattgat taatctagtg
     5101 attccctcta cgatatcatc gatgtaagtg aaatctcttt ccatctttcc atggttaaaa
     5161 atcttaattt cttttccaga taaaatattc ttggtaaaag agaaataggc catatctggt
     5221 cgcccaaatg gaccatagac agtgaaaaat ctcagcccag ttgttggtat tttataaagg
     5281 tggctataag tatgagccat taattcattc gattttttgg tagccgcata aaggctaact
     5341 ggatgatcca ccgaatcttc tgttgaaaac ggtactttcg tgttcccacc atatactgaa
     5401 ctagatgatg catataatag atgctttaca gggaagtttc ggcaggcttc aagaatatta
     5461 agaaaaccga taatattaga atcaatataa gcataagggt tttcaataga atatctaaca
     5521 cctgcttgag cagctaaatg aattactatg tcaaaacctt cactttcaaa tagagaatct
     5581 aatttttctt tctctaatag atccattctg ataaatttaa aattattata atcgtacagt
     5641 atcgttaatc gatcttcttt taattggaca tcataataat cattgagatt atcaatcgct
     5701 acaatttgat tttcagtttc cttgagtaat tttctagaca aatgaaaacc aataaatcct
     5761 gctccacctg ttattaatat tttcattttt attcatccac ctttttctga aaggaaccaa
     5821 tagggttaaa cccaaaattg ctttcttctt tatactcgta ttacatttat tatttaggca
     5881 taccccataa tttaagatat aacgaagttt atgatactat gcgtcttctc cattcactca
     5941 aaaaatataa cttaattttt tatgacttta aaaaggaaat cttctatctc caaatattta
     6001 ctaggatttg aaagaatagt ttagaaatca tttagggcta ataatgactt atgattctta
     6061 atttagcgct tttatataca ttattgccta aggtagtcac ttccttcatt gcatgtccgg
     6121 acttccacaa cacgtaagga aatgcgagct gatctcgccc actttctgag ttcaaatact
     6181 ctctccacca caaatctaga agattctcag cgattgaatt atttaaatca actaaaataa
     6241 ccgtggcttc agccaatcca aaattatcag gaaatccttc ttctctgtac ttactaattt
     6301 gctctataat actttcctta ttacctttgc caactgcaat taatgcatct gcctcagaat
     6361 ataaagattt tctttcacta tgtttatgca tacttaaccc aagtttggaa gaactgttca
     6421 gtaatccggt aatatcagaa gttattgtta cattaccatc aacatacaaa gtaaaatcgt
     6481 agtcagtaaa tagctcatta ggatgaaatt taatatatct attgatcaaa atattatttt
     6541 ttaattccga tatatattta ggtatctctc taatattcca atgctcactt gtaatcacat
     6601 tatctgtata caaatagtaa tctaccaatg gatgagaaaa atccggttct ttaatagaat
     6661 cataattacc agtgatacaa ctatacacag caatcctttt aggtgttcct tgattagtta
     6721 actctttttt tcttagttca tcactgcgat tctctctgtt tgaatagtct ttaataggcc
     6781 ccttaccggt catcgattta agggtatatt ttgctggcaa cagattatct cgttttttca
     6841 tttggttaag ttgattaatt ttatttcgta ttttcatgcc taaaatagaa ttattttttt
     6901 gatttaattc atgtatttca agatttcttt tttccaattc attgatagct aaactatata
     6961 attcttgtct tcccataaat ccctcatctc tatttttaag tgctatttat tcttactcaa
     7021 aaaaatagta aacagcctta aaaataatat tttctctaaa taatggtagg attccattta
     7081 caaaagcagt tcctcttata ttgatgaata tcaaacacac aatgataaaa aagaaatacg
     7141 caagcaagta gatataattc tttacttttg ttctcttata attactcatt aatacactgg
     7201 cataaataat ataatttaat acaaatatat tttgaataat tctaataaaa ttattactta
     7261 taagtaagaa aactatacta gtagaagcta acatattgat agaaaatata tatgagctta
     7321 agctgataac ttttttttcg taaatattca tttttgataa tttatgaact aaaaaaagat
     7381 ttgctgaaaa aaagagagca aacatcacta cgtgagaaat cgatgtactg tttctatcaa
     7441 aatatgcatc atacttcgta tttataaata ttgaactgat ataactttgt ccaatagaga
     7501 ataggagcaa gctaaggatt gtaacatatc cagtaatctt tttattaatt tttacaataa
     7561 aaaaagggag ataaaaatat gcaagattat ggaacagtcc agccactaag ataaaaaaaa
     7621 tatacttaaa tatagctttg ctgcctcgca atttagttag atgataaaaa gctagtaata
     7681 tgaatatcaa tgcgctaaac atacgtactt gtattgcttg tgaagtaaaa ccaaaaataa
     7741 aaaacaagaa caaaactaaa aaaacattag ttgtgaataa tctgatgaca tacaataaag
     7801 ctgagagtaa gacagtagaa ataatcaata aaaaaatctg atactgactg gaaataaagg
     7861 tacatacgta cataacagct ctgaatcccg gttcaatact atctgaacta aagtccatac
     7921 caaattgtgc tatcccatta tacaagacag aatatgcata ataatcagcg ttttgtgtgt
     7981 taaatgccat gatgatccac attatcaaaa gtagcacccc agacaaaatc ttgttcgatg
     8041 tactccattt tttttgtgaa acactctttt cttgattaat ctccaatgac gcctctgtat
     8101 ccatctcgaa ccccccttat agccgctcta aatctactga gcttatcagt ctcaaaagct
     8161 attgcaataa aaaatttcaa taatctttta ttcataaggc gggaaaattc cttatcactt
     8221 ttatacattc ttttcaccaa tgttgaattt cttgcaataa aatactttcg tgttgaatta
     8281 tgattgtcta ttttaatttt ttttgatcct atttttaaat tttctccatc tccaacgtga
     8341 tgataaaaat agacttgatt tgttacataa atgttatagc cattatttgc aaccctttta
     8401 caaaattcaa catcaatctg atcaataaaa aattcatcct tcatatttcc aacatcgtca
     8461 attaattcac ttttgaaaaa cattccagag gaatttgcaa atgctatttt catggaacca
     8521 ctagttgaag caaagtgttt ttttacgtat ctccctctct catttttgca ggtaaaagag
     8581 acagattcat cattgctatt ctcaaagtaa ttgggaacaa taatcccaat gtccttatct
     8641 ttcgcttcaa tattggtaaa ctcttctaat aattttaaag aaagattctt ttgtatgatg
     8701 gtatcttgat ctagtaaaag gatataatca catttctctt ttttggcaat ttcaatacct
     8761 atgttttgag ctgtcgcaag cccaatgttc tcattcattt tttttaaaat taagttttcc
     8821 atcatttcaa attgaaaaga gttaatatta tctgagccgt tatccacgat aataagtttg
     8881 ttctgtttct cttgtattaa cgtatccact aataacttta tgttattatc tggattgtag
     8941 gtaacaataa ttatcccaat tttatttttc atcgttcatc accttgtaca cttcattcca
     9001 tttcattttc aatacttgct cagagaatat agtggcgtaa atttttctaa cattttcatt
     9061 tatgttttta ttacactgat aaaatttcaa catctcattt tcatcaacgg tactattata
     9121 attgattccg acattatatc ttttaactat ttctttcgta tctcctgata aatttgaaat
     9181 tataggtaat ccaaacctaa aatactcaat cgatttcaga cttaaaccaa ttatggtatt
     9241 tgacttaaat aaatttagtc caaaatgaca ttt
//
LOCUS       DAYYFD010000104         9071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000104 DAYYFD010000000
VERSION     DAYYFD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.106"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..372)
                     /locus_tag="ACHA25_002495"
     CDS             complement(<1..372)
                     /locus_tag="ACHA25_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763745.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLS"
     gene            complement(669..3050)
                     /locus_tag="ACHA25_002496"
     CDS             complement(669..3050)
                     /locus_tag="ACHA25_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8763746.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3040..3687)
                     /gene="recU"
                     /locus_tag="ACHA25_002497"
     CDS             complement(3040..3687)
                     /gene="recU"
                     /locus_tag="ACHA25_002497"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8763747.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3751..4290
                     /locus_tag="ACHA25_002498"
     CDS             3751..4290
                     /locus_tag="ACHA25_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8763748.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4380..4799
                     /gene="gpsB"
                     /locus_tag="ACHA25_002499"
     CDS             4380..4799
                     /gene="gpsB"
                     /locus_tag="ACHA25_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8763749.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4821..5188
                     /gene="rnpB"
                     /locus_tag="ACHA25_002500"
     ncRNA           4821..5188
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA25_002500"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5397..6563
                     /locus_tag="ACHA25_002501"
     CDS             5397..6563
                     /locus_tag="ACHA25_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8763750.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6609..8105
                     /locus_tag="ACHA25_002502"
     CDS             6609..8105
                     /locus_tag="ACHA25_002502"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8763751.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8186..8869
                     /locus_tag="ACHA25_002503"
     CDS             8186..8869
                     /locus_tag="ACHA25_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8763752.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8878..>9071
                     /locus_tag="ACHA25_002504"
     CDS             8878..>9071
                     /locus_tag="ACHA25_002504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763753.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2896 a         1619 c         1898 g         2658 t
ORIGIN      
        1 tgataataaa aaaataccat ttataactaa acataaacta atggatttgg gcggtttcag
       61 gctcatcaga aaaatataga ttctggtgaa caacaaccat aaaaaagtga gaatcattgc
      121 atgagagctt ctttccataa attttccttt aagagtagtg cttaaaagta cagaaaaaat
      181 aataaatgca aggataggag caattaaagt aaatttattt aataataaac aaaatttttc
      241 agctttttta ttcttggaat aacgaggtat tgttttcgat aaaaaaagat ggtgcagaca
      301 aattatgata attaagcata ctatcagata actcaaaatg aagccttctt tcaaaaaatt
      361 attcaaaacc atatattgta ttttttattt gataataaca atgggattat taagaagtgg
      421 cactaaataa aagactagtg tgtatttagc cgatttaatt ataaacaagt aaatggataa
      481 agtatatgta atgataaaag aaaaatggat tttctaattt ttaagctaaa tgttagatca
      541 cacatgtata tgctggatag tcttataaaa aatcatgtaa tacagcttgg tacatgattt
      601 ttatttgtag ctgtttttta catagacaca aaaaagaaac cacgcttgta gacgtggttt
      661 cttttttact atctgtttcc agaagctgag gtgctgctgg atggtgtatg gtcatttgca
      721 gcatccgctc cgctagttgc tggctcactg gaagaagcag gaggagcaga tgaagacggt
      781 gtcgagcttt cttcggatga agaagaagga accgttgttg aagacgctgg ctcgcttgtt
      841 tctgctgtcg tgctttcttt tgaggactca gcagttgatt cgctttgact agtagagcta
      901 gagctttgag tcgttgtctc tgttgttgag cttccaggag tctggacata gctggaagat
      961 ggaatagttg ttgatggtgt aattgcattc gatctagctg tatattgatc tttgtagtac
     1021 agttcaccgc ccacacgaat taatccgctt ggcatctccc agtctgtatt tgtcacgtta
     1081 gctgaaacat actgcataag ctcgcgataa acatcagagg ctacgtgtga cgattctgaa
     1141 gtgacaggcg tcattttttt attgtaacct gtccacacgg aaatcgaata atttggcgta
     1201 tagcccgcaa ataggatatc tggatacacc ccagaagaaa taccaagttt tgcatactcg
     1261 tcatctgtgt aattggatgt tccggtctta ccggcttgat ataacccagc gatttgcgca
     1321 ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt ctgtgatcat ataagctgtt
     1381 tctggagaca tggctgtttt tccatctggc tcataagttt cttctgttcc gtcttggaag
     1441 acgattttat taacatattg aggtttgtaa taagtccctc cattggcgaa agccgcatag
     1501 gctgctgcca tttttaagga agaagctccg tattttgtcc catcttgttc ttccgtatta
     1561 ctagagatcg cattcgattg atggatcgta ctgtattcga tcccaagatt ttttaagaaa
     1621 gaagccactt tatctgaacc gacttcattg aataatttga ctgctggaac attacgggat
     1681 aagtaaagtg cttggcgtaa agtaatggtg cccatatatt gattatccca gtttcctaca
     1741 ggagtcgaag tcccttcata gttatacggt gcgtcagtaa tagtttttcc agtagaatat
     1801 ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt tcatcgtaga accaaaatca
     1861 cgggaagtat tcactgctaa gttgttgcct aaagttacgt cttctgcgat gtgacgtccg
     1921 ccaatctggg cttttacttt cccggtattg gtgtcgataa gggtggaggc aacttgcatc
     1981 tcgtcgtctg ggtaagagac atactgatcc gtgttgacga tatcatatag tttcttttgg
     2041 gcgtccagat ccaaattcgt ataaatctct aaaccgtctg tgtagacatt cttatctgtt
     2101 ttttcttgta cttcattgat gacttctttg acataattat cgacgatctt agtattgtca
     2161 tcggattgag ttagttcttg cagaccatcc gttaccggta cattaacagc ttgatcatat
     2221 tctgtttgag agattttttc gttttgaagc atcgtataca aaacagtatc tcgtcttttc
     2281 ttcgcctgat ctggataaac ataaggatca taggcggaag gggcttgagg catcccagca
     2341 agcaatgctg tttgcggtaa agaaagttct gataatttct taccaaagta catctcagaa
     2401 gctgtctcca tcccgtacaa gccgtttgac atatacactt tatttacgta gtaggtcaag
     2461 atctcttgtt tcgatttttt ttgttccaat cttacagcca tccacgcttc ttgggccttg
     2521 cgtttcagag tctgatcctc ggcactggtg gagaagaagg aaagtttgat caattgctgt
     2581 gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt tagagagggc agaaccgatg
     2641 atccgaatag ggtcaacccc gatgtgttta taaaaacggc gatcttctac cgacacgatc
     2701 gcatcttcta atgttttcgg taattcattc gcagaaattt tttcacgctt ttctgcgcct
     2761 aaatcttcga atagttcgcc atcttgtgta tatagtttgg aagatacggt agcgtctaat
     2821 tttttatccg tcaattcggg cgcatctttt gcgtaatacc agaaaagtcc tacacctgct
     2881 aaaaagagga tacagaaaaa tgagagtatt cctaaaagga tcttgataaa taatcctttt
     2941 ttatgtgttc cagaggactt tccagaacct ttgccagaat tcttcttcac actcttttta
     3001 ggagtaggct gtttctgtcg tctagagctt ctagtttgtt catttgccat tcttgctttc
     3061 tccttcaata gataatgttg ataaatagaa gtcaaccgcg tccaaataag gaatccgcgg
     3121 agaaatacca gttttgatct ctattcctag ttcttcgatc aaagataaag gtagtgattt
     3181 gcgtcctttt tccttttggg cgtcccagta agcaatcaaa tgaacactat tcaaataaaa
     3241 acagcgcttc aaagacgaaa accataacaa gacaaagcag accccatttt gttcgaggca
     3301 tgctttcata tgttcgattt gatgggcatg gaagttttta aagggaaaag atgttttgtt
     3361 cttggtttct tttgcttcaa agtccaagta aaagcctcga tagacgccat tatagtctgt
     3421 agttgaggct tgggtaaagt aggcttcctt gatcacagca gcacttctct taggatagtc
     3481 tactttcacg atttggatag gggtaggctt tttatggata accgccaacc cgcgggaaag
     3541 ataatattta ttactttcat tgattgcttc ttcaaagcgc atcccccgat tgccgaattc
     3601 agtgatctga tttctttttt cttgaatttt ttcgacggga gaggcattct tataataagg
     3661 ttggccattt ggatagcgca ataccatctt taatcacact cctaggccac attatagcaa
     3721 aaaagcggat agaaagaaaa ggaatctgag atggataagt caaaagtaat ctatgtgtct
     3781 gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg atcctaaaat ccaagttgtt
     3841 aaaaatgttt taaaaaaaga actccatcag ttagcagaag aagggctgga atgggtactt
     3901 gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa tagtagcgga attgaaagaa
     3961 gaatatccgg aactatctct tggtattttg tatccttttt ctgattttgg taataactgg
     4021 aatgaaggga accaagaaaa aaaacaatta gcagaacaat tagcagatta tgtcgaagct
     4081 gttagccatc aaccctacca atcacctagt cagctaaaga atcacactcg ttttatactt
     4141 gagcatacag tgggctgtct gctgatctat gatgaagaat tccctggaaa aacgaagtat
     4201 ttttttgaag atgcacagaa ttaccaagca gatcatccct acgaaattcg gctgatcagt
     4261 atggacgatc ttcaaaattt tagtgaataa tcgatattgt gtttgattta tcaacttttt
     4321 tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat gaaaactaga ggtgtaaata
     4381 tggcgaattt ggtttatagt cctaaagaca ttttacaaca agaattcaaa acaaaaatgc
     4441 gcggctatga tcccgttgaa gttgatgaat tcttagacaa tatcatcaaa gattatgaaa
     4501 cttacagcaa agaactcctt gctttacaag aagaaaacga tcgattgagt gcaaaagtag
     4561 ctcaattatc taaaactcaa ggagcagctc aaacacgcgt gcaacaaaca gaggcaccaa
     4621 agagcgcagc agtgaccaat tttgatatct taaaacgctt gtcaaatctt gaacgtgaag
     4681 tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa accagcacaa cctaatccaa
     4741 ataactacac aaatgcagac acaagcttag atgataatga aaaaacacgt caattttaat
     4801 ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc tgacttgatc aggctgagga
     4861 aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg tgcttagcga aaccataagc
     4921 taaggtactc tttggagtaa cggcaggaaa acaagctaag gcttcggcta tgcttaagta
     4981 tccttgaaag tgccacagtg acgaagcggc tggggaaacc catccggtgg aacgcggtaa
     5041 acccctcgag cgagcaaccc aaacaatggt aggggcgctt tttgtctgga agaagaacag
     5101 gacaaaaagg cagtataact gcagatagat ggttaccgct ttgttttttc tccctggaaa
     5161 agcaaagtac agaacatggc ttatagaaaa ccgcaaacat cagggaatta ggtcgtgaca
     5221 aaagtaatgc gttataagaa acaagaaaaa catttgaaag ctatttttca atacttcaga
     5281 tgaatcgttc ttattacaaa acaaactttt gtatcaccgg ttattcggaa tttgagtaaa
     5341 agtgtggggt aggactgatg tcccacctcc tttttttata aataaggaga ttgtgtatgg
     5401 aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag cgggctagaa gcactggttg
     5461 gtaaagaatt aagagatctt ggaatcgaat gccaagttga gaacggacgt gcacgttttg
     5521 aaggaacaat ggaaacgatc gctacagcta atttatggtt acgtacagca gatcgagtaa
     5581 aaatcgtggt tggggaattc gatgcgtata cgtttgatga attgtttgaa aacgtaaaag
     5641 cattgccttg ggaagatttt cttccactag atgcagcttt tcctgtagct ggaaaatcga
     5701 tcaagtcgaa actatatagc acacctgatt gccaagcgat cacaaaaaaa gcaatcgttg
     5761 aacgtctccg tacctattat caccgtccag cttctgtacc actgacggaa actggcgcac
     5821 attttcagtt ggaagtagct ttgctgaaag accatgtgat ggtaactctg gatacgacag
     5881 gaccaagttt attcaaacgt ggttatcgct tagaaaaagg tggagcacca ttgaaagaaa
     5941 acatggcagc agcacttatt atgttgacaa attggcgaaa agaccgtcca ttttatgatc
     6001 ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt aataggccat aatatcgcac
     6061 caggattcaa tcgtgagttt gcttgcgaat cttgggattg gttcagtcaa gaaatcatgg
     6121 aaaatgttcg atcagcagcc gaagaaaaag cagattatga tattgaatta gatattactg
     6181 gttcagatat caatggacgg atgattgaaa ttgccaaagc caatgcagaa gaaatcggat
     6241 taggctcttc gattaccttt aagcaacaag cagtcaaaga cttcaaaacg gataaagaat
     6301 acggggtcat cgtggctaat ccgccttacg gagaacgtct aggagaagaa gaaactgttc
     6361 ggaaattata taaagaaatg ggcgatgtat tccgtccatt aaaaacatgg agtaaatata
     6421 tcttgactag cgacttagcg tttgaggaat tttacggaca aaaagcaacc aaaaaaagaa
     6481 aattgtacaa tggtgcattg cgtacggatc tattccagta ttggggaact cgtccgccta
     6541 gaaaaaaaga gaaagacgaa taatcttact aattgattca ttcaattgga tgtttggagg
     6601 aatagggaat gaaagaaaaa gagtttttac aagaagtaaa agaaatcaat cttctacaac
     6661 aagctttagg aatcctcgat tgggatacac aaacaggtat gcctgaaaaa gctagtgcat
     6721 accgaagtga agtagacagc tatctttaca gcatttattt ttcaaaaaaa atcggtccaa
     6781 aaatcaaaga agccattcgc tatttttctg aacatccgga agaattgtcg gaagttggaa
     6841 ctgccgtttt tgccaaagta aaagaagagt atgatttgga acatcgcgta ccggaagatc
     6901 ttatgagaaa atatacagct gccgtttctt cagcacacgg acaatggcaa aaagctagag
     6961 aaacacaaca attttctgat tttcaacacg cattgaatca aaatattgaa ttaacaaaac
     7021 agttgatacc ttactggaga aaagatgaaa agacgaatta tgatgttttg ctgaatcaat
     7081 acgaacccgg gatgactgtc gaaatccttg ataaggtatt tgatcagctt aaagaaggaa
     7141 ttctttcgat ccgccgtacg ctccaagaaa aagggactga acctgaaaca gactttttaa
     7201 atcggagaat gacgaaaaaa caacagaaga gatttgtagt gaaagtaatc gaacaacttg
     7261 gttatgattt tagtcgtggt cgtttagatg acactgtcca cccatttatg actggaatta
     7321 atccgaatga tgcacgaatc acgacacgat ggaacgaaac agatttcaaa atggctgttt
     7381 ttggagtgat ccacgaggct ggtcatggta tgtatgaaca agacatcgat gataaatatg
     7441 cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca tgaatcacaa tcgttgttta
     7501 acgagatcat tattggaagt agtcgcagct tctggaaaaa gcaatatcct ttctttcaag
     7561 aatgtgcaga aggaacattc gatgatattc cttttgacaa attttatcgt tcattgaacc
     7621 atacaaaatc aagtttggtt cgtatcgagg cagacagttt gacttaccct ctccatatca
     7681 tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg ggtagatgtt gctgaactac
     7741 ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat acgaccaaca aatgatgtgg
     7801 aaggcatcct acaagacgtt cattggtcag gtgcaagctt tggttatttt ccctcttatg
     7861 cattagggta catgtatgcg gcacaattac ttcattcttt gcagcaagaa atagatgtgg
     7921 aaaatgttct agcgtcagac gattattctc caatcaaaaa ctggatgtca gagcatatcc
     7981 atcaatatgg tgcatctaga aaaccaaatc aattaatcat ggatgcaaca catgaaccgc
     8041 ttaatccgca atacctgatc gactatatga aagacctgta ttttaatgta tatcaggtaa
     8101 aataaaatac aatttacaaa gatagctgaa attcatctaa tctatagatg ttttcagcta
     8161 tttacttaag aaagaagtgg aacgaatgga taaattacaa gtgatcgctg attatagtat
     8221 ggaccaatta gcgcatgatc aaacaggaca tgggagcgat cataccaaaa gagttgtcaa
     8281 gttagctgaa aaaattttgg agacggaacc gcaggcagac cgctttgtga cgcttgcagc
     8341 agcttaccta catgatacga ttgatgataa agtcgttaaa aatgagaacg aggcaaagca
     8401 acagcttcgt gtattccttc gctctttgcc aatagtagag gaacagatct cgatgatttt
     8461 ttccattatc gaaaatatgt ccttttctaa aaatctctct gaagcggccg aactatctct
     8521 ggaaggtaag atcgttcaag acgctgatcg tatcgaagca ttaggagcaa tcggcatttt
     8581 gcgtactgct tatttcggcg gtggacatgg gcacccgatt tttgatcctg aactgtatcc
     8641 gcaaacattc aaggacaaag aaaattaccg aaaaggcacg acagtgatca atcattttta
     8701 tgaaaaactg ttcttgctgt ctgataaaat gaatactgat tacggatatg aagaagccaa
     8761 acgaagagaa accttcatgc gtgaattctt agaagaattt tttctggaat ggtctattga
     8821 cgatgaaaat tttactccag acaattggat caaaaataac aaaaaatgat ggaggttgtg
     8881 acagaagtgg tcagctccaa gatatttcgg atcattttcc gtaaaagtta cttctggaac
     8941 actgtttacc aagaggtcgt gagcctgccg ttcagctccg agtcacaagg aacaatttca
     9001 aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg attcggaatt ggcaggagaa
     9061 caccgtttat t
//
LOCUS       DAYYFD010000105         8940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000105 DAYYFD010000000
VERSION     DAYYFD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.107"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHA25_002505"
     CDS             complement(13..1152)
                     /locus_tag="ACHA25_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8763754.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHA25_002506"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHA25_002506"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8763755.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHA25_002507"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHA25_002507"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8763756.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHA25_002508"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHA25_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8763757.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHA25_002509"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHA25_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8763758.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHA25_002510"
     CDS             complement(6166..7257)
                     /locus_tag="ACHA25_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8763759.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHA25_002511"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHA25_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8763760.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHA25_002512"
     tRNA            8800..8871
                     /locus_tag="ACHA25_002512"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DAYYFD010000106         8913 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000106 DAYYFD010000000
VERSION     DAYYFD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.108"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..768)
                     /locus_tag="ACHA25_002513"
     CDS             complement(139..768)
                     /locus_tag="ACHA25_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8763761.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(761..1879)
                     /locus_tag="ACHA25_002514"
     CDS             complement(761..1879)
                     /locus_tag="ACHA25_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8763762.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1881..2513)
                     /locus_tag="ACHA25_002515"
     CDS             complement(1881..2513)
                     /locus_tag="ACHA25_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8763763.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2524..4341)
                     /locus_tag="ACHA25_002516"
     CDS             complement(2524..4341)
                     /locus_tag="ACHA25_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8763764.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4354..5118)
                     /locus_tag="ACHA25_002517"
     CDS             complement(4354..5118)
                     /locus_tag="ACHA25_002517"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8763765.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5146..5844)
                     /locus_tag="ACHA25_002518"
     CDS             complement(5146..5844)
                     /locus_tag="ACHA25_002518"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8763766.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5856..6635)
                     /locus_tag="ACHA25_002519"
     CDS             complement(5856..6635)
                     /locus_tag="ACHA25_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8763767.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6651..7595)
                     /locus_tag="ACHA25_002520"
     CDS             complement(6651..7595)
                     /locus_tag="ACHA25_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8763768.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7641..8420)
                     /locus_tag="ACHA25_002521"
     CDS             complement(7641..8420)
                     /locus_tag="ACHA25_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763769.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            8763..>8913
                     /locus_tag="ACHA25_002522"
     CDS             8763..>8913
                     /locus_tag="ACHA25_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8763770.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRI"
BASE COUNT         2442 a         1831 c         1511 g         3129 t
ORIGIN      
        1 agaaattgtt ttcttatatg tgagtaatca gatacaacat taaaatattc gatactgttt
       61 tccgtcagaa tttcctctaa tatattcgtt cgactgatat aatcaaaagg atgaataagc
      121 agccactttt ttttattctc actcatctat cttcgcaccc tcaaatttct cagtagtccc
      181 acttgctact gatgttattc catcacggac aataactttc tttagcgtta aaaaaaatat
      241 ttttatgtct ttcatcattg aaatgttctc cacatattca atatcttttt caaatttttc
      301 tttccaacta attgcattac gaccactgac ttgagctaat ccggttaaac ctgggcgcac
      361 aaaatgtctt tttcgttgtt cattactgta tagtgaaaga tactcaacta acagaggacg
      421 cggtccaata atactcatat ctccttttaa aatattccat aattctggta gttcatcaag
      481 actagtcgaa cgcaatattt taccaaattg agtcaatctg atatcgtctg ataataaatt
      541 accttcttca tctctttgat ctgtcattgt tcgaaattta tacatcagaa aaatcttttc
      601 atgttttcca ggtcgttgtt gtttaaagat cactggcgat ccaagcttta ttcgaactag
      661 gattgcaaca aacgtcatca gtggccataa cattattaaa cctaaaattg aaaaaagaat
      721 atctatcaaa cgtttaaaga acttttcgta gatacctcta ttagacattt tgcatcactc
      781 gcttaattgt attagttact cgatacaaat catcatcggt cattttagaa tctgaaggta
      841 aacagatacc tttttcaaac aatgtctgag caacatctgt accaatatac tcatacttat
      901 cgaaaactgg ctgtaaatgc attggtttcc atatcgggcg ggcttctata ttatttttat
      961 ctaactcatc aataagatga ttcgtattta ttccctttac tgttatgaca cttaaccaat
     1021 aatttggttc attccaatta ttttctggca tgaattgtat atttgacaat ttagacaatt
     1081 cttttttata aaaactatga atatatcgtt ttttctcaat tctttcattt aaaactttca
     1141 attgtcctcg accgatacct gcaacgatat tgctcattcg ataattatag ccaatttcat
     1201 tgtgttgata atggcgtgcc ttctcacgag attgcgtagc ccaaaacctc actttttcca
     1261 ccttttcttt gtcattagac acaagcattc cgccacctga agtcgtgatt atcttatttc
     1321 cattaaaaga ataaattcca tagtcgccga atgtaccagt atattttcct ttatatgttg
     1381 ttcctaatga ttctgcagca tcttcaatta aaacaacatt atgttctttg catattttag
     1441 taatttcatc tagttcagct gataaaccgt acaaatgcac tactaataca gcttttgcgt
     1501 taggatattt ttctagcgct gttcgcaaat gttcagggtt catattccaa gtttcgtaat
     1561 cactatcaac aaatactgga atagctcctt gatagatgat tggattggca gtagctgaga
     1621 acgtcaaaga ctgacaaatc actatatcat ctttaccgac cccagccgct tttaaagcta
     1681 gatgtatagc tgctgttcca gaagatagtg cagctgcatg actagcaccg acatattctg
     1741 ctaattcctc ttcaaaacca ttgacattca tacccaaagg agccacccaa tttgtatcaa
     1801 atgcttcttt tacatactct tgttcatatc cttcatcact catatgagga gatgctaata
     1861 atattcgttt attttccaac ttaatcccat ctttctatga ctttggtagg tacgccaaca
     1921 tttttagtct tattattaat attttttatt actacacttc ccgcacctat aatgttcata
     1981 tttccaattc ttagattttg tattatttta gtacctactc caatttcatt tttatctcct
     2041 aatagaacat gtcctgaaat atttacactt ggaaaaattg aattataatt accgatttga
     2101 gtatcatgtc cgatagtaga gccgatattg atcatattga aatttcctaa aactacgtca
     2161 gcagtgtaag tcgtataggg catcaaaata ttgccaattc ctaactgaac atttatgccc
     2221 actagtgcac ttgatgcaac taaattaggg aaaatcaatt tattattttt atttatcttt
     2281 tgatatattt tctctttgat atctggcgaa gcgattccca gaaaaatagc agtttcttct
     2341 tctaattcta ataacgagtc tattgttccc aatacagttt ttcccactaa ttcttgattt
     2401 tgcattttta acgaatcatc gacaaaacca atcaattcat atctagagaa actttctagt
     2461 agaaaagcaa tctctttgcc aaatccacca gctcctacaa taattgcttt cactttactc
     2521 cccctactta ttcgaagcat tcgcaaaagt gacaatgtct ttagccaatt gttcatcgtc
     2581 ttgcggcagg ctcttgacaa attccatcac tgtttgcatg gaataacctt tgatgtttcc
     2641 cacaaagatc ttctcgtaaa ctgcttcatc gtttctttcc ttatctaaca atagttcttc
     2701 atatagtttt tcccctggac ggatacctgt ttcaatgatt tcgatctcgt cttcgctata
     2761 tccgctcaat cggatcatgt tttttgctag atctacgatt ttcactggtt cactcatatc
     2821 taaaataaag atttcgccgc cttttgctaa cgccccagat tgaataacta aacgacttgc
     2881 ttcaggaatc gtcatgaaat atcgggtcat acgaaagtct gttacagtga ctggccctcc
     2941 ttgagcaatt tgttcccgga atactggaat cacactaccg cgagaaccaa ggacattccc
     3001 aaatctgaca gctgagaatt tcgtacatcc ttcttcattc aacccagtca cgatcatttc
     3061 agcgattcgt tttgttgaac ccatgacgtt tggcggattg ttggctttat ctgtcgagac
     3121 catgacgaag ttttttactt tcgctgcttt ggctgcttct gctacatttt ttgtaccgaa
     3181 aatattgttt ttgactgctt cttttgggtt atattccatc aatggtacat gcttatgagc
     3241 ggctgcatgg tagacgatat ctgggtggta ttgttccatg atctcattga ttttcttgcg
     3301 atcttggata tccgcaatga tcggcacgat ttctgtagga cacttttgat gatgcgtgcg
     3361 taattcacgg tcaatcaagt aaatcgagtt ctccccatga cctaaaagta atagttttgc
     3421 agggtcaaat tggatgattt gccggcagat ttctgaaccg attgatcctc ctgcaccggt
     3481 gacaagaatg actttgtttg tgatctggtc tttgatggat tcgatatcca actctacctc
     3541 atctcttccc agtaattcca ctacgtcgat ggtttttaat tttgatacgc tgattttccc
     3601 tgaggcaagt tcttccatag aaggcatcgt attgactttc acatgagcag attcgactaa
     3661 ctcgaagatt cttcgtaatt tttttcttga taaagaaggg atagcgatcg ttaccatttg
     3721 gatgtcatat ttttcgatca attccggtat atcttttaat gaccccagta ctttttttcc
     3781 tgaaagatag gtatttcttt tattcggatc atcatccacg aatccaatga catggatatc
     3841 ttgagccgtt ttagaaccaa gaaagctgtt gtataaaata cgtccacctt cgccggcccc
     3901 aacgatcaag gtatttttag cggaatctgt tgatagatac ctcatatttt ttgtttcgat
     3961 atagatacgc cagatcaatc ggctgccaat gatcagtaat agcgaaagta ggtatgcaaa
     4021 gatcactaaa cgtaaactgt attgtttatt tatgaaaagc aatatgatga tacttgaggc
     4081 cgcacttgca gataacgaac caaaaatcgc aatgatctct cttaaatttg tataacgatt
     4141 gattcgagta aaaactttga ataaactacc atagaataaa taaaaaccaa tcgacaaacc
     4201 gatagagatt aaaataaggt ccattggtat agctacaaac ggcttcatga atttagctgc
     4261 tgcaatattc gcagcaatca acaacaagct gtccacaaat accaatgtgg caatttttct
     4321 tcttcttgtt aatacaaaca ccctacattc cccctaaaat aaaccaaact tcttcttttt
     4381 tacctcttca tagactggat aactgatctc atccccgttg atcaggtcct ttgcgacttg
     4441 tttcatctgt gctacttttt ccttaccaaa gtccttggcg atttgctcat aacattcttt
     4501 taagtaaaag gtacgtttag agaccccatg ggcatcggaa gcagccattt gcacgaggtt
     4561 atgtttgacc atcattttgg ctgttttctg gatgtctttt ccaaatacac cgacataact
     4621 tggtgcggtg agttgagcca agcatcccat atctaggaat ggcagcaaat gatttgggtc
     4681 ttctcgaaaa tgcgcgttgc gttctggatg gacgatcact ggtgtctttc ctagttgtaa
     4741 caactcaaaa aagagccatt ctgcatataa cggcacttcc agtgtcggaa attcgattag
     4801 aagatatgta tcatctaaat ctgtaaataa aatttcatct cggtggatgt cttcaatcag
     4861 tgttccggta atacgtactt cttgtccttc caataacgtc aaaggcaatt gtcttttctc
     4921 taattctgct tgtaacgaag cgactagaga aatgacttgt gatttttcat tgctgtattt
     4981 tccattattg tgatggggtg tgcataaaat atgtgtgatc ccttgttgaa tcgctttttc
     5041 agccatggcg atactggcat ctatgttttc tgctccgtca tcgattcctg gcaggatatg
     5101 gcaatgtaga tcgatcattt ctcttgtctc ctctcttttt atttcttaat tcccgtagta
     5161 atagccagca tctctgctgt gttctgctcc attgtataca acaccaagga ctcgcgcttg
     5221 taccatatct aacagttctt ttgctttagt caatgattct tttcgtgacg tattttcccg
     5281 tacgaccaat aaggtgccat ccgtttttga agacatgatc tgtgcatctg ttacggcaac
     5341 gactggtggc atgtcgaaaa tcactacatc aaatagttgt cttgcttctg ctaaaatctg
     5401 atccattctt ggcgaactta gtagttctga tggatttggt ggtttaggtc cacttggcag
     5461 aatcgacaaa ttgtcgactg gtgttcgttg gatctcatct gctacacttc ctgatgaact
     5521 aagaacagta cttaatccgc ttgcgttgtt caacttgaac gttttataga tgacgggctt
     5581 acgtaagtct gcatcgacta gcagtacccg ttgaccagat ttggcaaaaa caacggcaat
     5641 gtttgctgca gtggtggatt ttccttctcc aggacctgat gaagtcacga caatcgtttt
     5701 gatttgctga ccagctgcgg aggcaaactg gatattcgtc cggatcgtac gatattgttc
     5761 ggcgattggt gaagatgggt ttgttaatgt gatcaaactg actgcatgag tttgagttgc
     5821 tttttgtttc tgtgttcgtg ccatttttct tcctcctaga ctcttgagcg gcttctgcgt
     5881 gatggggctg ttttatcgtt tgtagctttt ggtcgtgcag gagctgatcc ttttttacgg
     5941 ataccagcgt tcaattcttt tgctgacatt tctggaactg ttcctaagat tgggaatcct
     6001 aatgtatccg tcacatattt ttcatctttc actgtccgat caagtaattc gaataagaat
     6061 gccagaccta cgcctaccat cattcctaac actaagccaa tcactagatt caatttatta
     6121 tttggcgaaa caggtgtcgt attggcaact gcacctgaaa taatcgagat tttatcgaca
     6181 ttcatcacgt ctttggcgtt ttcttggaaa acttgtgaag ttgtattagc gatctgttga
     6241 gcagtcactg cattcgtgct tattgcctgg atcgagaaca tttgtgaatt ttgtgactga
     6301 ttgacggaga tcgcatcttt gatttgtcca gcagtcattt tcacattgtc ttctgtttct
     6361 aaacgatctt tgacttcatt tagtactaaa tcactgacga tcatatcttt atacgtattg
     6421 atcatttgca ggttcgtatt gacatcattg acatttgctg tgtctgattg tggcagtgtc
     6481 acgatcaatt gtgcttgtga actgtatttt ggtgtcaaga tgaaaaatgt tacgattcct
     6541 gagatggcca agccagcaaa cattgagatg atgattgtta ccaagtgttt gcgtaatgtt
     6601 tcaaaaattt ctttcaagct gattgtctct tccatttttt ctccactttc ctatttattc
     6661 tctagattca tttgatcttt taattctttt ttcacacgat caagttcttg atcgctgact
     6721 ttttgatagg agatattatc ttgcatgaag ccttctcctt gcagctgatc ttgcttgatc
     6781 gtaccaaatg cctcccgata atctaaagcg atttttttca tttcatcaaa gctcatgtct
     6841 gttttcatat tcgactctag agcattcaaa acggattgat agttgctgac accgtttaaa
     6901 ctcaatactt tttttgctac accttctacg atctttcttt gacgctcttg tctaccatag
     6961 tcaccatttg gatcttcata acgcatacga gaataagata atgcctgatc cccgttcaat
     7021 gtaattttac cgattggaaa gtcatagcca tcttgtgaga aagtgaggtc attattgact
     7081 tcgataccac caactgcatc aacgagttcc tttagccctt tcatattgat agagacatag
     7141 tgatcgattg ggatgtctac atatttttgt agggtatcca tcgccatggc agctccgccg
     7201 aatgcgtaag cgtggttgat cttgtctttt gtgtcacgac ctacgatgtc gacatacgta
     7261 tctcgaggaa tgctgacgat cgttgtctgt ttgtcttcag gactgactgt ggcaaccatc
     7321 atcgtatctg atcttccttg atcgacccga cctaaatctc cagtatcaat gcccatcaat
     7381 agaacagaga aagattcttg tttgtccaga tcaactggtt tcgttcgttc tccttgggtt
     7441 cgttccactg attcgtaagt tttctcaata gaatgtgaga gatccagata taattttgct
     7501 cctactccta caattgctaa tactaatacg ataagaactg ctaaaataat cagaaatatt
     7561 tttttccctc ttgattttct tcttcgtctt gccatagttt cctccgtttg ctaaccttgc
     7621 ttgaatcaac gttttaactg tcaataactt atattgctat agcccatttg ctgagcaaca
     7681 gtttgtgctt ttgttgcgta tgctgcgctt attttttcat tccggatctg ctctatttga
     7741 ctgagcgccg tcaaatattg atcgtaagta gcggtggcag tcacttgacc atccttcaac
     7801 atcgtatcga ataactgatc tagttgcgtg acttgcgcga tctgagtatc agcgaatcct
     7861 agatattgca taacaaccgt tttccaagga ctatctttaa aaaagctcat attttcacgt
     7921 acacgagcga catctgcact tgtcaccttt tctttgatga cgacatcgtc tacagcttgc
     7981 tgccagtttg atacattttt agtaaataaa tcgttgacag ctgtctggat cttcagtttt
     8041 gattctgcac tatctaactg tgtcacgatt gctttcttct catcttggat tgcaacagct
     8101 tctttaggca attctgattc tttgatgtcg taatcatctg ctgaaagttt gacagaggat
     8161 agcttatttg acaaacttgt gattgtttct tctgttattt cagggtttag aaaatttttt
     8221 tcttttgtac tatacagctt gtctacttct tttgataaag aagtcaaggt gtctttttga
     8281 ttggtgatgg cagattctgc ataattgatc cgttgctgat gttgccgctg attattgata
     8341 tggtaaccaa acaaacagat cgcaatagca cctgcaccta ccgccacagc agtcaatgac
     8401 ttgacgaatg gcttttccat agattcactc ctaagtattg atgatagata aattaagata
     8461 taattcacat acataaaaat gacagctttt tattttaaaa aacataatta aattaatata
     8521 ttcacagaaa tatttgtaaa caagttttcc ctatataatc ataccacaaa ttgttttctt
     8581 gactagggca atttacgatt taaaagtaaa taaaacatta ataagataac aattaatcga
     8641 tttcattttt atttccttaa ttttcataga gagtgtaaaa tattttgtgt aaatagaaaa
     8701 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc
     8761 atatgaacga ttttactaca gaaattctaa agactctagc gaacaaaggc gatttgaatg
     8821 aattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg
     8881 ctttcctcga ttacgaaaag tacgatcgca ttg
//
LOCUS       DAYYFD010000107         8720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000107 DAYYFD010000000
VERSION     DAYYFD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.110"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..658
                     /locus_tag="ACHA25_002523"
     CDS             62..658
                     /locus_tag="ACHA25_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM8763771.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            639..1085
                     /locus_tag="ACHA25_002524"
     CDS             639..1085
                     /locus_tag="ACHA25_002524"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8763772.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1428..1751
                     /gene="relB"
                     /locus_tag="ACHA25_002525"
     CDS             1428..1751
                     /gene="relB"
                     /locus_tag="ACHA25_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8763773.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2071..2298
                     /locus_tag="ACHA25_002526"
     CDS             2071..2298
                     /locus_tag="ACHA25_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8763774.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2314..3480
                     /gene="hemW"
                     /locus_tag="ACHA25_002527"
     CDS             2314..3480
                     /gene="hemW"
                     /locus_tag="ACHA25_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8763775.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3715..4758
                     /gene="hrcA"
                     /locus_tag="ACHA25_002528"
     CDS             3715..4758
                     /gene="hrcA"
                     /locus_tag="ACHA25_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8763776.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4852..5415
                     /gene="grpE"
                     /locus_tag="ACHA25_002529"
     CDS             4852..5415
                     /gene="grpE"
                     /locus_tag="ACHA25_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8763777.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5466..7295
                     /gene="dnaK"
                     /locus_tag="ACHA25_002530"
     CDS             5466..7295
                     /gene="dnaK"
                     /locus_tag="ACHA25_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8763778.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7446..8612
                     /gene="dnaJ"
                     /locus_tag="ACHA25_002531"
     CDS             7446..8612
                     /gene="dnaJ"
                     /locus_tag="ACHA25_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8763779.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3051 a         1419 c         1877 g         2373 t
ORIGIN      
        1 tttttcgatt tttcaagtta tctaatgcaa ttatattgat atcattagga gctgcattgt
       61 tatgaatcca acattgatta ctaagaaaga attactaaaa aaattagata tttctactgg
      121 tgtattagct aatttaatta gaaatggcat gccaaaagag ggagaaatgt ttaatcttga
      181 taagattatt acatggcgag aaaattggtc taaaaacata ttaggtgagt tagaagttgg
      241 tagagtatat actaacaaag aaatttcaga gaagtttaaa tgttccaaac agggtggaat
      301 gagaagatct catcaaacta atactcttgt actgtttagt gatcaaacag gaagtaatgt
      361 ttataaagat aagtggttaa atggtatttt acaatatacc ggtatggggc taaaagggga
      421 tcaagtattg gataagaatc aaaataaagt tttagccaat tcaaaaagta attttgtaaa
      481 gattcattta tttgaaacat ttaaaccaaa agaacacaca tatctgggag aagtatattt
      541 agcaggacaa atatatactg ttaatgaaaa agatagttcg ggcaacagca gaaaagtata
      601 taaatttcca ttagctttaa taaatcaaga gcaattgatt gaagataaag atatataacc
      661 aggaagaaaa tcaaactaga catattcgta atttatctaa tgctaaatta gaggaagaag
      721 caagaaaagt tagtaattat aataaaaaaa tggctgcaaa atatgttaac gaagaggagt
      781 tttgcttctc aaaaacaaaa atatataaac gtaatccctt cgtatctgaa tatgttaaac
      841 gacttgcaaa aggtatttgt caactttgcc aagaaaaagg gccatttata aaagacggtg
      901 taccttattt acattgtcat catatagaat atttgtcgca aggtggaaaa gatgtgattg
      961 aaaactgtat cgcgttatgt cccaattgtc atgctagaat tcatgaactt gagttacctg
     1021 acgataaaga aaaattgctg aagatcgttg aaaaaagaga aaatagcttt tctaaaatac
     1081 cgtaatttat ttttatagga caaataagct aatgtgagct atgccttcta tataagacta
     1141 agttctatta taagtaacga agtaattctc gttccttcct gtagtaatat tttttaaata
     1201 acctataggc ttcttcaaaa gtacatcctc cagtaaattc taagtactgg aggattcttt
     1261 ttctagtgtt gctttactca tgaaaaagta gttggctact tcatcaaaat ttctgaactt
     1321 ttttaatgta taataaagag ctcgatcatt tactacaagg atgctagctc ctacattttc
     1381 acagtttttt agcaagtaaa tttttctaaa atcacatcag gaaatgcatg agattcttta
     1441 agagaggcaa ttatagggct atgattacgg ttcgtgtaag tgacgccaaa aaagaatggc
     1501 tttcttatat ggctgaattt tatgggatta ctttatctga acttctaaaa aactattcga
     1561 tggaacaact agaagatgaa tacaatcgtc aaacggctga aattgcccat aaacattgga
     1621 taagaagtgg gaaaacaaca gtattcatgg aagaatcctt aatgaatttg gtgggctgga
     1681 atgacctgcc gtttaaaatt cacgcccgaa attcagaagc agctctgatt ggcaaatacg
     1741 tgtattggta gagatagaag ataatcaatt cgttgttatc gctattcaag tagggcaccg
     1801 aagaaatgtc tatgattaag agactgttta tttttacaaa aaaataataa ttggaatcta
     1861 gttaaaactc ttgaagtgag agatttgact agattttttt atgaataaaa taaggacaaa
     1921 attgtttagg aagaatcatg agtgtaaaaa aatcatccaa gaagtcgatt cttggatttt
     1981 ttagtggatt tgttcgccaa atacgtttag tatgcgtata tgagaggttg taagcaaagc
     2041 gttgtggtct gagcacttaa ggaggaatct gtgaaaatag cttttcatat ttttgcagat
     2101 tttgacttaa tgccgaaggt cagctttgcg attaccgttt atcgagaggc tgtaaaagca
     2161 ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct tgattctaaa
     2221 tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt cgaaaacagg
     2281 cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa tcccgcagaa
     2341 aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta ttactgtgac
     2401 tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat gctgctgaat
     2461 gaaatgagat taatgctgga aagtcatcct acagcgaatt tggaaacact ttatgtagga
     2521 ggcggaacac caacttcttt atctgctaaa caacttgatc gactattatc aggtgctaga
     2581 gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc tggtgatttg
     2641 acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca atcgtttatc catgggagtg
     2701 cagacatttg acaatcgctt attgaaaaaa attggacgta aacatactgc agaagatgtg
     2761 tatcaaacaa tgcaattttt agaagcagaa aattttacca atgtaagcat cgatttgatt
     2821 tatgcattac cgggacaaac actagaagga tatcgggata ctttggaaaa agctttggct
     2881 ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac aatgttcatg
     2941 aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac cagaatgttt
     3001 gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc aatatgaaat aagtaacttt
     3061 gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga ccactatttt
     3121 gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa caaaggacca
     3181 atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga agaagaaaaa
     3241 ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa aaaaacaggg
     3301 attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt gtacggtgat
     3361 gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg tgtttatttg
     3421 acaccaagag ggactttttt ggggaacaat gtgtttgagc gctttttatt tgaaaaatag
     3481 tttgaagaat atttgttttt tatcgaaact tgaaaagaaa aagtttagaa agtttgacaa
     3541 tgaggtattg aagtattgat ataactggat ttacatcaat tatttagaat tagcactctt
     3601 gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag gagaagggct tcttgctata
     3661 tttataagtg aattagcact tagaatatat gagtgctaat gagaggtgag gaagatgtta
     3721 acaaagcgac aaaacgacat tttacgtctg atcatccaac actatacgag tagtggcgtg
     3781 cccgttggtt ccaaaacatt aatgtctgag ggagtcaagg ccagctcagc aacgattcgc
     3841 aatgatatga aagcattaga agatgaagga ttgctgttga agactcactc ttcttctggg
     3901 cggattccat ctgctttagg ctatcgttat tacgtggatc atttgttaaa acctgctcgc
     3961 gtagcagaag atgatttgct gctgattcgt cagtctctag gcagagaatt tcatgaaatc
     4021 aatgagatta ttaagcaatc tgctgagata ttatctgagc tgacaagcta tacgactttt
     4081 tcattgggac ctgaaataaa agaccgccga ctgactggct ttcgtatcgt acccttgaat
     4141 aatcgacaga ttattgctat cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc
     4201 actcttcccg aatatatggg aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac
     4261 cgtctagtcg gagatcctct gatgacggtg taccataaac ttcgtacgga gattccgatg
     4321 attcttcata aatatttcca gacgacagaa gggatgtctc agttatttga caccgttctt
     4381 ggtcatgttt ttgaagaaaa ggtttatgtc agtggacaaa tgaatctctt ggattatgag
     4441 ccccgtcaag atcttgatca gttcaaatcg atgttttctt ttatgaaaga ctcagacgga
     4501 cttacacaaa tgatcactcc gatggatagc aatattcata tcaagattgg tgctgaatta
     4561 ggcaatgatt tattgcagaa tatgagtatg attcaagcaa gttacgagat agatggtcat
     4621 ggaagaggta caatagcctt gttagggcct actagtatgc cttattctcg gatgttcggt
     4681 ctagtcgatg tttatcgaag agaactagca gctaaacttg ctgattacta ccgctcgctt
     4741 gacttgtcaa actcgtaatg tgaataacga agctatttgc agctgatcgt tctctctttc
     4801 ttagtaagag caatcaaaca gctgactgaa taatgaaagg aagactgtat cgtgaaagaa
     4861 aatcaagaag aactagataa agaaatgaca gatgctcagc cagaaccgga aatcgatgtc
     4921 gaagctgcag aagattcggg tatttcagaa gctgaagcgg aagaattcga aacagcgaaa
     4981 ttaaaagctg agttagaaga aatggaagat aaatacttgc gggctcgtgc tgaaatcgca
     5041 aatatggcaa atcgaggaaa aaatgagcgt gagcaattac aaaaataccg ttcacaagat
     5101 ttagccaaaa aattacttcc atcaattgac aacctggaac gtgcattagc cacagaagtg
     5161 tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt
     5221 aacgcactgg aagaagaagg aattgaaaaa atccctgcta aaggagaagc ctttgatcct
     5281 aatcttcatc aagcagtaca aactgttcca gcgactgaag atacaccagc cgatacgatt
     5341 gtagaagtac tacaagaagg ctacaaatta catgatcgcg tgttaagacc aactatggtc
     5401 attgttgcac aataattgtg cggatataaa acttacactt tcaattaaag aaggagacgt
     5461 taattatgag taaaattatc ggtattgact taggtacgac taactctgca gtagcagtat
     5521 tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg aaaccgtaca acaccttctg
     5581 ttgtatcatt caaaaatgga gaaatccaag taggggaagt tgcaaaacgt caagctgtga
     5641 caaacccaaa tacgatttca tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag
     5701 atgtagaagg taaatcatat acaccacaag aaatctcagc aatgatcctt caatatatca
     5761 aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag
     5821 cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc tggtaagatc gctggtctag
     5881 aagtagaacg tatcgtcaat gaaccaactg cagctgctct tgcctatggt ttagataaaa
     5941 cagatcgcga cgaaaaaatc ttagtattcg accttggtgg cggtactttt gacgtatcta
     6001 tccttgaact aggagacggt gtctttgatg tattgtcaac agctggcgac aaccatctag
     6061 gtggggacga ctttgataac aaaatcatcg actacatggt agcagaattc aaaaaagaaa
     6121 acagcattga cttgtctcaa gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa
     6181 aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat cagcttgcca tttatcacag
     6241 caggagaatc tggaccattg catttggaaa tgacattgac acgtgcaaaa tttgatgaat
     6301 taacagctga cttagtagaa cgtacaaaag taccagtacg tcaagcgtta aaagatgcag
     6361 gtctgtcaca atcagaaatc gacgaagtta ttttagtcgg tggatctact cgtatccctg
     6421 cagttgtaga agcagtacgt aaagaaacag gaaaagaacc aaacaaatca gttaaccctg
     6481 atgaagtagt tgctatgggt gctgctatcc aaggtggcgt gatcactggt gatgtgaaag
     6541 atgttgtttt acttgacgtt acaccattgt cattaggtat cgaaacaatg ggtggagtgt
     6601 tcactaaatt gattgaccgc aatacaacga ttccaacaag taaatcacaa gtattctcaa
     6661 ctgcggcaga taatcaacca gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg
     6721 ctgctgacaa caaaactcta ggaagattcc aattgacaga tattccagca gctcctcgtg
     6781 gtattccaca aatcgaagta acattcgata ttgataaaaa cgggattgta aatgtatctg
     6841 cgaaagatct aggtacacaa aaagaacaaa aaatcacgat caaatcttct tcaggtctaa
     6901 cagacgaaga aatcgaacgt atggtgaaag atgcagaagc aaacgctgaa gcagataaag
     6961 ctcgtaaaga ggaagttgac ctacgtaatg atgtagacgc attgctattc tctgtggaca
     7021 aaacattgaa agaattagaa ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag
     7081 ctcgtgacga attaaaagcc gctgttgaag caaacaacat cgaggaaatg aaaactaaac
     7141 gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa actatacgaa caagctgcac
     7201 aacaacaagc tcaagaaaat ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg
     7261 ctgattttga agaagtcgat ggcgacgata aataatcagt tcactgaatt tcgagagaag
     7321 gccaaagttg ctactttggc tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt
     7381 tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg atgagaacca ataggaggaa
     7441 agcctatggc aacaaaacgt gattattatg aagtcttagg gctctcaaaa ggagcatcag
     7501 aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa ataccatcct gacatcaata
     7561 aagaacctga tgcggaagaa aaattcaaag aagtatcaga agcatatgag attttaagtg
     7621 acccgcaaaa acgcgctgca tacgaccaat acgggcatgc aggcacagat ccaaattacg
     7681 gtgctggtgg agctggtggc ggatttggtg gattcggcgg cttctctggt ggcggcttcg
     7741 gtggttttga agatatcttc gactctttct ttggaggagg cggtcggtca gtcgatccaa
     7801 atgcgcctcg tcaaggtgct gatttgcaat atacggtcga cttgtctttt gaagaagcga
     7861 tttttggtgt agaaaaagaa atcaagtata atcgggaaga aatctgtcat acttgtggtg
     7921 gaaacggtgc aaaaccaggc acacaaccaa ctacttgtca taaatgtcat ggtgcaggta
     7981 cgatcaatgt agaacgccaa acgccacttg gacgagtgat gagccgtcaa acttgtgatg
     8041 tttgtcatgg cacagggaaa gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac
     8101 atgagaagaa agcacattca gttaaagtga atgtaccagc tggtgtagaa gaaggacaac
     8161 aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg cggaccattt ggtgatttgt
     8221 atgtggtttt tcgtgtagaa gaaagtgata tctttgatcg tgaaggttct gaaatttact
     8281 atgaattgcc tttaagtttt gtacaagcag ctttggggga tgaagtccaa gtaccaactg
     8341 tccatggaga tgtgaaattg aagatccctg ctggaacaca gacaggtacg aacttccgtt
     8401 tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag
     8461 taaaactgat cacaccgaaa aatctaaatg aacaacaaag agaagcatta cgtgcattcg
     8521 ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga cggtttcttc gataagttta
     8581 aagatgcttt tggcgggaaa aagcggaagt agtttttcta gtaaaaatat aaagtagact
     8641 tgcccaaatt ttctatttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga
     8701 acaatcatac gttcgcctgg
//
LOCUS       DAYYFD010000108         8684 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000108 DAYYFD010000000
VERSION     DAYYFD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.111"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..1656
                     /locus_tag="ACHA25_002532"
     CDS             25..1656
                     /locus_tag="ACHA25_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8763780.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1718..2359)
                     /locus_tag="ACHA25_002533"
     CDS             complement(1718..2359)
                     /locus_tag="ACHA25_002533"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8763781.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2545..2621
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2641..2949
                     /gene="rplU"
                     /locus_tag="ACHA25_002534"
     CDS             2641..2949
                     /gene="rplU"
                     /locus_tag="ACHA25_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8763782.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2976..3320
                     /locus_tag="ACHA25_002535"
     CDS             2976..3320
                     /locus_tag="ACHA25_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8763783.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3333..3626
                     /gene="rpmA"
                     /locus_tag="ACHA25_002536"
     CDS             3333..3626
                     /gene="rpmA"
                     /locus_tag="ACHA25_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8763784.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4060..6867
                     /locus_tag="ACHA25_002537"
     CDS             4060..6867
                     /locus_tag="ACHA25_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8763785.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7058..7549
                     /locus_tag="ACHA25_002538"
     CDS             7058..7549
                     /locus_tag="ACHA25_002538"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8763786.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7652..8641
                     /locus_tag="ACHA25_002539"
     CDS             7652..8641
                     /locus_tag="ACHA25_002539"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8763787.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
BASE COUNT         2787 a         1481 c         1902 g         2514 t
ORIGIN      
        1 tttcatgaaa aaggagttag ataggtgttc aaaaattatg tttcctcact aaaaaaagaa
       61 ttttcaggtt atgatctttc aagatttcag aaagatcttt tggctgggat cacagtagct
      121 gctgtagcat tgcctttagc cttggctttt ggggtatcaa gcggagctga tgcggccgca
      181 ggtttgatta cggctatcat tgccggtatc gttattggcg gtttatcagg cggtttttat
      241 caaatatcag gtccgacagg tgcgatggca gctatcttga tgtctattgc ggcaacaaag
      301 ggaatgcaag gtgtattgct tgctactttt ttagcgggtc tatttttact tgcagcagga
      361 cttttccgat taggaacact gacttctttt atcccagcac ccgtaattac ggggtttact
      421 tcaggtattg ctatcattat tgcattaggt cagatcgata atttatttgg tgttcactcg
      481 gaaggtacaa acgttattga aaaactaagt agttatcaaa cgataggatt tccgataaac
      541 acagcctctt tgatcatggg agggctagtc attttaggga tgtttatcta tccaaaaaaa
      601 tgggcgaaac gaatgcctag ttcgctgctg gctatcattt tggcaacagc agttatgcta
      661 ttgttccatt taccagtagc aactgtgggt aagattccac aaacattgat cagtagtaat
      721 cgtttgaata tgggagattt tagcttttct gccttgcaac aagtggcagt tccagctatt
      781 agcattgctt tattaggaat gattgaaagc ttgctatgtg gtgcttcggc tggccgaatg
      841 gcaaataagc ccttagatag caatcaagaa ttaatggcgc aaggaattgg aaatctgttg
      901 cttcctttct ttggcgggat tcctgctact gcggccatcg cccgtacaag cgtggccatc
      961 aaatctggtg cacaaactag attagctggt atgatacatg cagttgtctt actgctctcc
     1021 atgcttgttt ttgcgccagt gatgtcacag tttccaatgc cagcacttgc aggtgttttg
     1081 attgtgacgg cttggcgtat gaatgaatgg gaaacgatca aggagctatt tactaataaa
     1141 tattggtcag cagttctatt gtttatcctt acgatgattt gtacagttat ctttgattta
     1201 agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat tttttgttgt caaaagcgct
     1261 gctatcacga tttcattaga aacgatcgat tggcaaagaa tggggctgcc agaaacggaa
     1321 aagctgaata actggacagt agtgtatatt agtggtccac tgttttttat gtcttcagaa
     1381 aaactcaaaa ataaattgga agaactaaat catcacgatg gtgtcatttt ctccatgcga
     1441 ggcgtaccaa gtattgattt gacggcacgt gcagtttttg atgaatttca agaaaaagca
     1501 gagcaaagag gacaaacgat tatctatact tccttgcagc cagaagtaga aaaacaactt
     1561 tctcacttgt gggaacatac attcacgcat caacatcaaa cagttgctca tgcgttgaca
     1621 gctttacatg cgaaatatac tgcaattagc cattaaaaca atgaaaaaga acagacaaga
     1681 ctgtaaactg aagattttat ggtcttgtct gttcttttta atattgctgt aaaagtgaag
     1741 ctagtacttg ccagtcttga tggataacag gtacaaggga gcgattgtag aaaatagctg
     1801 ctggatgaaa aagtggaaga attcgatagt ttttttctga ccattcgtag gcatcctttt
     1861 gttctgttaa ttgtaagaca ggccctgtat agagttgacc atgatttttg gtaaccgtat
     1921 attctttgcc aagtaaacgt tgaagaccaa tgtttccaag tgtcgcaatt aattttggct
     1981 ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc aaatacttcg gaacgagtag
     2041 gtgtccgatt tggataaaca gtttctgact gttgtgtccg tttattgatc cgatgagtca
     2101 cgcgatacgg gcggctcctt acagcactgg tgatatacac ctcttctcgt gttaagccag
     2161 ttgaagaaag tgcctgcatc aattcttttc cagcttgacc gctaaaagga acatgcgtat
     2221 caatctctgt tttccctggg gcttctccca cgagcatcag tttaggatga agaggtcctt
     2281 gcccagcgat aaatccttct acaggacggc cgttcattct taaagaagct tcttgtatca
     2341 gttcttttgg gtattccatt tatgctcctc ctttttcttt tattataaga ataaaagaaa
     2401 cacaatgcga ctattttatt tggtagaact gtgaggatag aaaagcagaa gtattgcttg
     2461 aatttttgtt ttactttttg attttaaatt tagttgacta acggttgctg ttctggtaag
     2521 ctagtactgt tgtaattgtg gactccacag ctacaaccgc tcagaacaag taataagcac
     2581 agcatatgct gtcacttcgg atggcgagtc taagtatatc agaaggaggt gctttatagc
     2641 atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag ttgaagtggg acaagcaatc
     2701 tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg tttttgacga agttatctta
     2761 gtaggcggag aatctacaaa agtaggcgct ccaactgttg caggtgcaac tgttgaaggt
     2821 actgttgaaa aacacggcaa acaaaagaaa gtcgttactt tcaaatacaa acctaaaaaa
     2881 cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa aagtggttat cgacgcgatc
     2941 aacgcttaat agcgtttgag aaagaaggcc tatatatgat caaaggaaca tttaagcgaa
     3001 atgacgcagg tcagatcgtt tcattcactt tgaccgggca tgctgatgct ggtccttatg
     3061 gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat cagcactgtc aacgggattg
     3121 cctcattagc cggttttgag ccgattgttg aaatgaatga agaagaaggc ggttatcttt
     3181 ataccgaagt cacttcagga atgacacaag aacaaaacaa tattgctgag attctattag
     3241 aaaatctttt attaggatta caatcgatag aagctgaaaa ctcagaatat atccaaatta
     3301 aaacaattaa tgataaatag gaggtgcaga ccatgttgtt atctatgaat ctacaattat
     3361 ttgcccacaa aaaaggtggc ggttctacat ctaacggccg tgactcagaa tcaaaacgtc
     3421 taggtgctaa acgtgctgat ggacaaacag ttacaggtgg atcaattctt taccgtcaac
     3481 gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg tggagacgac actttatttg
     3541 caaaagttga tggcgtagta cgtttcgaac gtaaaggccg taacaaaaaa caagtgtctg
     3601 tttatccagt agctcaagaa gcttaattga atcttatgat taaagaggtt gtgacaagac
     3661 ttttgtcaca gccttttttg tttataaaaa aaaaagttgt agtggaggct acgaagttta
     3721 ctttgtcttt ttacacttaa tatttttttg ccaatttgga atcaggatgt tttgataact
     3781 atcgttttca attgatggaa gctatattgt ttgctatcag tgaaataatt atatggtaaa
     3841 cagtgttctt tttcttgttt ccattataaa aatcgtatcg ggatttttcg ttcttttaaa
     3901 ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa aatcaggtaa atagttgacg
     3961 aaacagaaat aaatacatag tattatgttt tgaagcctaa gtacaatagt ctatagaaaa
     4021 ggagcgatac cattggcaaa acgcattgac cgaatttata tgtatgtcaa agaaaaaacc
     4081 gctcatttaa caaaggcaga atatgatcaa ggagtaacaa cacaggaaat tgctgaagtg
     4141 ttagggattc agcgaacaaa tagcagtaaa gatctgaatc agctagtgcg cgaaggaaaa
     4201 ctgataaaga cagacggacg accagtacgt tacatatatc aaacactcgt tacgcacgta
     4261 aagccagtta caaaacatgt agtcagttac aaagaaacgc cggtctctga agaaaaaaat
     4321 acactccaaa tagatactaa ggatatcttt gcaaaaatca ttggagctaa cggcagcatg
     4381 aaaaattcag tggaacaggc aaaagctgcg attttgtatc cgccaagagg attaaattgt
     4441 ttgattacag gacctaccgg atcaggaaag acttattttg cacacgcgat gtatcatttt
     4501 gcaaaagcgc atcatgtaat agatgaggaa acggaactga ttgttttcaa ttgtgctgat
     4561 tatgccaata accctgagct tttgatgagt catttatttg ggtacgcaaa aggtgctttt
     4621 acaggcgcag atgaagaaaa aatggggatt atcgatcaag cggatggcgg tatgctgttt
     4681 ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa tgatctttta ctttatggat
     4741 catggaactt acagtcgtct tggggaaaca acgaaatccc atgaagcaaa tgtacggatc
     4801 gtaggtgcga ccacagaaga cccaggttct tctctattag aaacttttgt tcggcgtatc
     4861 ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg caaatgagaa agtcgattta
     4921 gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc gaaagatttc tttgacagaa
     4981 gatgtagcaa aagcattgat cggcagtgtg acctatggaa atataggaca attgaagtca
     5041 aatatccagt tagtatgtgc acgaggattt ctaaatcata tgcaatcacc cgaaatctcg
     5101 atcacaatcg atgatttgac agaagggatt cgtagcggat tgattcaatt agcaagcaac
     5161 cgcagtgcaa tggcagagct ttctaaaata ttggagccaa agataactgt agcaccaaat
     5221 gatacgatca tgaaaatcca atcggactcc tatgagttac cttataatct atatgatatc
     5281 atcggagata aagcagcttt actgaagtcg gatggtttgg atcaagaagc aatcaatcat
     5341 ttcatttcta cagatatcaa catccatctg aagtctttct ataaagacca tggtttttct
     5401 tttaatgcag acagcaaatt agcagaattt gtagatccga aaatcataga agtgaccaat
     5461 cagatttatg taatggtcaa aagatcttta ccatatgagt ttcagcaaaa ctttatttat
     5521 gctatgagct tgcatatcag ttcttttttg aaaaaaatca acttaggaga agaacggcat
     5581 acgaatgata acatccgtga gatggctttt gattttccag atgaatatgc tgttgcgaaa
     5641 gaagtgcgta gttatatcga aacgtatttt caagtggaga ttccagaaag tgaagactat
     5701 tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag caagcggcag aatcggtgta
     5761 gtcgttgcag ctcacggaaa cagcacagca agcagtatgg tccaagttgt ccaacagttg
     5821 ttagatgcag agaacgtacg agcagtagac atgcctttgg atatggatcc gaaaacagca
     5881 ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag gaagcggggt catactctta
     5941 gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc aaagagacac tggaattcca
     6001 gtacgtactg ttgatatggt aacaacttct ctagttttgg aaactgtacg aaaggtctca
     6061 gtattaggga cagatctaga tatgttgcat gaatcattga aaaattttcg aggttacgca
     6121 gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta ttcataagaa aaaagctgtt
     6181 ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa tcaaagaatt gatcgaacga
     6241 gcggtaaaca agcgtcaaga aacagaatta gaagttctag ctttgtcgat tgtggaagtg
     6301 aaagaagaac tgccagttat ccaaaaagaa tatcagatca tagctactac tgggattact
     6361 gatttgaata tccaagcccc atttattcct ttagaacgtt ttattgatca aaatatcgaa
     6421 gtcattttgg atcagttgct gatggaatca gaattggagg aaactgaatt catctcccta
     6481 gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct ctgataactt catctttatt
     6541 aatggtagta agttaatcga tccaatgtgg caattttcta cacaaatctc tcagacgacg
     6601 ggaatcggcg atgaagaata tggattcaaa atcaacctag tgatgcacac agcaggaatg
     6661 atcgaacgta ttattcggaa tgaaccattg actgtggaag aaaacgaatt gacaaataca
     6721 acgaatgatc ctctttatag ccaattggca gcctcagttg ttctgttgga agatcagata
     6781 aaagtaaaag tgccgataga agaaatgtat tatctgctac gtctagtcca taatcagtta
     6841 gataaaaaag aatacactgt gccatgaata cacaaaaacg atacactttt atagtgtatc
     6901 gtttttgtgt attggttaag aatcctttaa attcaattag tgtatcgtaa aaaagttggc
     6961 acgctttttg cttatagtag tgtgtgctag gaggtggttg ttcacacaga aatgataggg
     7021 gtttctgtga agtagtgagg aagaaggagt tttgcagatg gatattcgat tggcacgtat
     7081 agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg tcgaagcaaa caggtgttga
     7141 acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa ttgagaaagt ttctactaaa
     7201 agaagctgca ccgccgggga tcacagcaaa tgtaataacc gtgcagaaaa tgattcaaat
     7261 ctatcatagt ggattattat cacgagcaaa agtgatgctt ttattcacca atccccaaga
     7321 tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg ttgaatatcg gcggtatgag
     7381 ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca gtaaatcaac aagatattga
     7441 ttcctttcgt tacttgaaca gtcaagaagt ggagttagag attcgaaaag tacctgctga
     7501 caggaaaatc cttttgatgg aactactagc gaaaatcaaa aaggtgtagc aagaatggtt
     7561 gttggaaaag gtcaaataat tctgttgtaa tggttagcaa ttattaatat ttttgaattt
     7621 gttacaacaa aagtaaatag gaggtataaa tatggtaggg attatcctag caagtcatgg
     7681 acaatttgct gaaggaatct tacaatccgg ttcaatgatc tttggcgaac aagaaaacgt
     7741 caaagccgtg attttgaaac caagtgaagg tccagatgac ttgagagcca aattggaaga
     7801 agcggttgct tcttttgata atcaagacga agtgttattc ttagttgatt tatggggcgg
     7861 aacaccgttc aatcaatcaa acacattatt cgaagagcac aaagataaat gggcaatcgt
     7921 cagcggtttg aatttaccaa tgttgattga agcctacgca tcacgctttt caatggaatc
     7981 tgctcatgaa attgcagcac acatcattga aacagcaaaa gacggtgtaa aagtaaaacc
     8041 ggaagaatta gaaccggcag aagcaccgaa agcagcagtt gaagacgcac aacctaaagg
     8101 agcacttcca gaaggaactg tggtcggaga cggaaaaatc aaatatgtat tagcacgtgt
     8161 tgactcacgt ctgttacatg gacaagttgc gacagcttgg acaaaagcag tgcaaccaaa
     8221 ccgtattatc gttgtttcag atgcagtatc aaaggatgat ctgcgtaaga gactaatcga
     8281 acaagctgct cctccaggtg taaaagcaaa tgttattcct attagcaaaa tgatcgaagt
     8341 agcaaaagac cctcgttttg gaaataccaa agcattgctt ttatttgaaa atcctgaaga
     8401 cgtattgaca gctgttgaag gtggcgtaga catcaaagaa ttaaatgttg ggtcaatggc
     8461 gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct atgggacaag aagacgttga
     8521 agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat gtacgtaaag tacctaatga
     8581 ttcacgcgat aacatggatg atattttgaa aaaagcaaaa gctgaattag caaaagcgta
     8641 ataaataata ttaaaattta taggaggctt atcatgtcta ttat
//
LOCUS       DAYYFD010000109         8414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000109 DAYYFD010000000
VERSION     DAYYFD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.112"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..1074)
                     /locus_tag="ACHA25_002540"
     CDS             complement(106..1074)
                     /locus_tag="ACHA25_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8763788.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1306..2139
                     /locus_tag="ACHA25_002541"
     CDS             1306..2139
                     /locus_tag="ACHA25_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8763789.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2173..3012
                     /locus_tag="ACHA25_002542"
     CDS             2173..3012
                     /locus_tag="ACHA25_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8763790.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3038..3598
                     /locus_tag="ACHA25_002543"
     CDS             3038..3598
                     /locus_tag="ACHA25_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8763791.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3797..5518
                     /locus_tag="ACHA25_002544"
     CDS             3797..5518
                     /locus_tag="ACHA25_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8763792.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5683..6825
                     /locus_tag="ACHA25_002545"
     CDS             5683..6825
                     /locus_tag="ACHA25_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8763793.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6841..8052
                     /gene="thiI"
                     /locus_tag="ACHA25_002546"
     CDS             6841..8052
                     /gene="thiI"
                     /locus_tag="ACHA25_002546"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8763794.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2886 a         1455 c         1744 g         2329 t
ORIGIN      
        1 tacgaaaatt gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa gaagctattt
       61 ttgtcacagc ctctcttttt aagtttagct attagataat gatgtttagt tttggataaa
      121 cgaaatcaat tcttcacccg acaaaccata gtttacagca atcaatagtt ttaaacgtgc
      181 tttttgacta ttaatactgt tacagaaaat aatccccatt ttttctagtt cttttccccc
      241 gccttcataa tcataaacag gctctgcgat tccattgaaa caacgagaaa caagtactac
      301 agggattttt tggacaagaa gcttttccaa agcagctaag gcttgtggag ggagattccc
      361 cgcacctaaa gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg cttccaataa
      421 gtccccttgc atcccagcat atgctttaac gatcgggata gtcccatcta cagcggaaat
      481 gtccagtcgc tttgtttcta aaagttcctg caagaataaa attcgattct tagttactaa
      541 gccgatggga cccaatgtag gtgttcggaa agtagcaaca tttgtggtat gggtctttgt
      601 cacgtagcgt gcagaatgga tctcatcgtt catgaccacc aaaaccccct tatctagcgc
      661 ttcttcacaa cttgcgacac gaatcgcact ttcaaaatta taaaggccgt cacttccaag
      721 ttcattactt gaacgcattg ccccagtcaa aacaattggt aatttgttgc caatcgttgt
      781 atcaagaaaa aaagcagttt cttctaatgt atctgtaccg tgtgtgatca ccactccgga
      841 tatacctgat gcataagcgg acaagatacg ttcttttaat accaacatgt gagaaggcat
      901 gatgtgtggt gagggaagat tgaagatatc ttcaactaat aattctacat gcttggggat
      961 tgtgatttct gcatctaata atgggtttgc cacatcagga ctaacaccgc ccgtgagcat
     1021 gttttctttc atggcgatcg ttccgccggt gtgtagtaca agtatttttt tcattttaca
     1081 gacttcctta cgaataattt tgcatagccg ttaaatctta ttgtatacta tataaaagag
     1141 ttttgctatc agtgaagaat aacgaatagc ctaacttatc ataaaaaagc tgaccttatt
     1201 aaaatggaaa agactcaacc ttgacctcct aatacaatgg agtatctggt tgagtttctt
     1261 ttacaagaaa gcagccaagg aaatcaatca cgaaggtggc cggaaatgat cagaatcgga
     1321 ttaacatcgt ttaaagaaca tgacaaatta actggtaaaa agaataacac tctttatgaa
     1381 tatgcagggt ttctaccctt agtggaatta gatactgcct attatggaat tccccgaaag
     1441 tcctcagttg agaattggat aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc
     1501 tatcaaggaa tcagcggtca aggagactgg aagccttatt atcagaccga agaagagatg
     1561 attgcgcatt ttttagatgt tatgtcgcca atggtagatg ctggaaagat gttttgcttt
     1621 ttagtccaat tcccctcatc attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa
     1681 cttagaaagt ggtttgaggg ttatcctgtt tctatagaat tgcgggatta ttcttggtac
     1741 agcgatgaat ttcttgaacg tacaagaaac tttatgtcta gagccgcttt ttctttagcc
     1801 atcatcgatg agccacagtt gctgaataca acgattcctt ttgatccatt tgtcaccaat
     1861 ccgaactttg ctctttttcg attccatggt aggaatgcgc aaggatggca agcaaatgat
     1921 aaagagtggc gtaaaaaaag aacgttatat cgttattcag aaaaagaact gcaagaacta
     1981 gcacatgaag tcaaagtagt tgattctaag acaaaagagg taggtgtgat ttttaataat
     2041 aattctggtg gtgatgcggc tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag
     2101 ccagatggac tcaatccaac ccaaatggat ctgttttaag ggcttatttt attcaagaaa
     2161 ggggcgaagt ctatgatcaa agctattttt tttgatattg acggaacatt ggtcaccaat
     2221 cgttcaaaag cgttggaatc aacaaaacaa gcaattgaat atgctcggaa aaacggcatt
     2281 ttatgcggtg tagcaacggg gcgcagccca gtaaagatca aagaaatcat tgatgaattg
     2341 gaattagata tgtatgtcgt gtataatggt caactggttt ttactgcaga tcgcacgatt
     2401 atcgaccacc cttttgaaca aaaagtattg gaacatattg ttgaatttgc agatgagaac
     2461 catcgacaaa tcgtttttgg tgcgagaaat cggttagatg gatcaacgac gatgctatta
     2521 ggacaatcga tttttatcaa gcgactagtc agctttttac cgcgtaaatt cccggtacgt
     2581 ctgatgaaaa aaatcctcca ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg
     2641 ttagagatat taaaagaacc aatttatcaa tgtatattgc tcagtccgga gtcagaacag
     2701 aaaaagttag aacatcgctt tcccgagtgt acgtttcagc gttcaaatac gtatactgta
     2761 gatatcatcc caaaaggtgg ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc
     2821 gaaattgaaa tggaagaaat tatggcattc ggcgatcatt acaatgatat cgagatgctg
     2881 aaaggtgtag gaattggagt ggccatgggg aatgctcaga tcgaagtaaa acatgctgca
     2941 gactttgtga ctcaatcaaa tgaagaagat gggatatacg aaggactgaa acacttcggt
     3001 ctgatttatt agtcaatgaa agaaggaaaa aatcagtatg gaagcagaga caccttttca
     3061 aaaagtagaa acattccaca aaacttttga tccaagaaga ccagagatac ccacggcttt
     3121 tacagaaaat gaagctcata cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt
     3181 tgcagctact tcacctaata aagaacaact gcatgaagct gttatgctgc tgcacgaagc
     3241 attagataaa gcagaagaaa aaatacttgg taaaacacag tggggaagtg atccgctggt
     3301 tgaacaagta gatgcactga cagatatcct ttactttact tatggttctt tttcattact
     3361 aggaatagtg cctgatcgaa tattcgaaat cgtccatcag gcaaatatgg ggaaattatt
     3421 tccagatgga aaacctcatt atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg
     3481 gcagatggaa tatgctccag aacctaaaat cgcggcagaa atccaacgtc agattcaaga
     3541 agcgattgct aagaagaaat ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc
     3601 agagtttcac tatttttgtt atctttatac gatggaaaaa caaaagattt tcgtttaagt
     3661 tagtgaatgc atgaaaatag tatgctataa tctagaatag atgtcttttg cacgagagac
     3721 gtattatttg gatttatgcc aagcagaacg ccatctgttg cataaaagat gcatactgaa
     3781 aacggaggaa ccataaatgg gaaataatgt tatcatcgga atcgtcattg caatcattat
     3841 agtcgccatt gtcgtctttt tggttagtta cttgatgaag aaaaaaaacc aagatcgatt
     3901 ggatgcctta gaaaaacgca aggaagaatt atttgacttg ccagtgattg aagaagtcga
     3961 tgatgttaaa aaaatgcatc tggttgggca aagccaaaat acctttcgtg aatggagtca
     4021 aaaatggaat gacatatcta cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt
     4081 ggaaaacctc aatgaaagtt ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga
     4141 agctacaatg gaccaaatgg agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt
     4201 gcgggaaacg gaagaacgca actcactagc tgttcagcag gcacttgacg tctatgaaga
     4261 actgaaagaa aaagtcagag aagacgagca tgaatacgga ccagctcttt ttgaattgca
     4321 aaaacagatc aaagtgatcg aaaaagaatt tacgcagttt gtgactttaa acacttctgg
     4381 agaccctgtt gaagcccgcg aggttttgga taaagcagaa aaacatacgt atgaagtgga
     4441 agctctgatg aaaaaaattc caccgctgta tgaggagctc cataccactt tccctgaaca
     4501 acttgaagaa atcagtgata cttacgagaa attgatggaa gaacaatatg tattcccaga
     4561 agaagatctt gctgaagata tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa
     4621 tcttgaaaaa acagaagttg aaacggtcga atttgaaaac cgtgaaacag ctgatttgat
     4681 tgattcgtta tatgatatct tagaacggga aatggaagct cagagatatg taaaaactaa
     4741 ccaatcaacg attgcagagt atatcaaaca tacaacaaag aataaccgtc aattattgat
     4801 tgagctggat cacacatctc aaagctatac attgaaccac aatgaaattg gccgtgtacg
     4861 aggtttccaa accgaagtgg aagaaatgga acgtcaaaat gaacagatga ttccacagat
     4921 ccgtcaacac gaaattcctt attcagaagt gcgcacgttc tataaaacag tcttcaaagt
     4981 cttagaagat atcgaaaagc aacaagtaga gatcgacgac tcattacatg aattgagaaa
     5041 aggcgaaaaa gaagcgcaag aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa
     5101 acgttttgta gaaaaacaac gtttgcctgg cttgccgaat gattacctag aattcttttt
     5161 tgttgctact gaccgtattg aagaactaag tgttgtcttg aataaaattc gagtaaacat
     5221 ggaagaggtc aatcgtttag ttgctctatg tgaagaagat ctagaattat tagataagaa
     5281 gacacatgat ttagtagatg cagcagcatt aacggagcaa atgctccaat atgcaaatcg
     5341 ttatcgtcat acacacgaag aagtgagaga agcaattgat aaaagctact atttattcaa
     5401 taaagaatat cactatcaag aagcattaga cgaaatcggt acagcattag agcgagtaga
     5461 acctggtgct ttcaaacgca tcgaagattt ttattttaat catccagact tagtataaag
     5521 caaaaaagtt agtaaaccag caagagcaag acggattatt gtcttgttct tgtctttttt
     5581 tgtatatttg aaaattgaaa caagtgaaaa tttcacctgc tcttgaaagc accttggaca
     5641 accgttataa tagaaaagaa tttcaaaaaa gaggggtttc ttatgatata ttttgataac
     5701 agtgcaacaa ccccaatctt tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg
     5761 attattggga atccatcaag cttgcatgat ttaggcaatc aagctaaccg gttgttgcag
     5821 caagcaagaa agcagatcgc tgatttgatc catgtagaac cagaagaaat ctattttact
     5881 tcaggcggaa cagaaggaga taactgggta ttaaaaggaa cgatgatcga aaaaagagaa
     5941 tatgggaatc atatgatcat ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa
     6001 caattaaaag aattaggaat cgaagtaagt atcgccccag tggataaacg cggatttgta
     6061 cgtgtcgacg aattgaaaga attaataaga aaagagacag tattagtctc tgttatggct
     6121 gtgaataatg aagtaggatc gattcagccc atccaggaaa tcagtgatct tttggatgat
     6181 tttcctaaaa ttcatttcca tgtagatgct gttcaggcga ttggaaaagt accattctcg
     6241 caatggttga cagaaagagt tgattttgca acattttcag ctcataaatt ccatggccca
     6301 cggggaactg gtttcattta ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt
     6361 ggagggcaag aaagaaatca gcgaagtggg acagaaaatg ttccaggtat tgtggctatg
     6421 gttaaagctt tacgtttatc attagagata aaggaacaaa aacctgagca gacacaacgc
     6481 ttaaaagtag ctctaatcaa tgcgttgaag acgtatgaga aagtgacgat tttttcagaa
     6541 ggaactgact atgcaccgca tattttatgt tttgctctaa aaggaattcg cggcgaagtt
     6601 ctcgtccatg ccttagaaga aaaacaaata tttgtttcta ctactagtgc ctgctcctca
     6661 cgtaaacata tggcaagcag tacattgcac gcaatgcatg taccaaatga tctggctaca
     6721 tctgctgttc gaatcagctt agatgaaaac aattcgcttg cagaagtcga gcaatttatg
     6781 attattttca agcagcttta taagaaattt tctgcaatca attaaaagag gagtgttctt
     6841 gtgaaataca gtgaaattat ggtccgttat ggggaacttt cgacaaaagg aaagaatcga
     6901 aaaagcttta tcatgcaact agcacaaaat gtcagacaag ctttgaaaga ctttccagaa
     6961 atcaaaatcc atgctgatcg ggaccggatg catcttttat tgaatggcgc agacgggcag
     7021 ctagttattc caaaactagc aaaaatattt gggatccaaa atttctctcc aagtattcgt
     7081 gtagaaaaag atgtcgaagt tttgaaacaa gctgttcaag acatcatgaa agaaatttat
     7141 actgggaaag aaacatttaa aattatcgct aaaagaagcg atcaccaatt tgaattgaca
     7201 agtaatgaat tgaatcaaac attaggtaat gctgtttttg atattttccc gcatatccaa
     7261 gttcaaatgc gtcaaccaga tattccatta cgtgtagaga ttcggcgtga tggcgcttat
     7321 ctatcgtatg aaacgatcaa gggagctgga ggattgcccg taggaacaag cggaaaagga
     7381 atgcttatgc tttctggggg gattgattct cctgtagcag ggtactttgc catgaagcgg
     7441 ggagtggaaa ttgaagctgt tcattttgct agtccgccgt atacaagtga acaagcactg
     7501 caaaaagcaa aagatctaac agccaaacta gcaccttatg tgggaacgat ccattttatc
     7561 gaagtgccat ttaccgagat tcaagaagaa atcaaaaagt cagtgccaca aggatattgg
     7621 atgacaatta ctcgtcgaat gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat
     7681 ggtttagtca ttttgaatgg tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt
     7741 atggtggcta tcaatgaagt aacgaatacg ccaatcattc ggcctgtagc gacaatggat
     7801 aaacttgaaa tcattgaagt agcagaacag atcgatacat ttaatctttc tatccagcct
     7861 tttgaagatt gctgtacgat ttttgctcct ccacagccaa aaacacgccc tcaactggaa
     7921 aaggtccatc aatatgaaga gcgtttagca atagatgaaa tgatccaacg agcactagct
     7981 ggcttgaaga tggaagagat acaagcagaa tcgtctaatg aaaaaactga agaattttca
     8041 gaattgctat aagaggttat gaaaatagtg ttttgaccag agtattagag aaacaaaacg
     8101 aaaaaatagc tccatgtatt ttaccgtttt tgaactaatg tcgaagatta gctgattgaa
     8161 taccatttat caagaagtta tggaaaagcc ggttcttttt tactaactga aaaataaaat
     8221 tttatttaga gatcaagcta cagttgataa atgatataaa aaagagaatt tcccatgaaa
     8281 tagcgaggtt gtgacagaag tgggcagctt caagaaataa gaaggaattt acgaaaattg
     8341 cttttcaaat ttttgtgaaa tttcggctta tttttcgaag aagttacttc tggaacaccg
     8401 tgtatcagag gttg
//
LOCUS       DAYYFD010000110         8229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000110 DAYYFD010000000
VERSION     DAYYFD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.113"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACHA25_002547"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACHA25_002547"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8763795.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACHA25_002548"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACHA25_002548"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8763796.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACHA25_002549"
     CDS             2591..3376
                     /locus_tag="ACHA25_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8763797.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACHA25_002550"
     CDS             3389..4240
                     /locus_tag="ACHA25_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8763798.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACHA25_002551"
     CDS             complement(4364..4816)
                     /locus_tag="ACHA25_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763799.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACHA25_002552"
     CDS             complement(5281..5649)
                     /locus_tag="ACHA25_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8763800.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="ACHA25_002553"
     CDS             5799..6281
                     /locus_tag="ACHA25_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8763801.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="ACHA25_002554"
     CDS             6318..7328
                     /locus_tag="ACHA25_002554"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8763802.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="ACHA25_002555"
     CDS             complement(7443..8156)
                     /locus_tag="ACHA25_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763803.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DAYYFD010000111         8093 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000111 DAYYFD010000000
VERSION     DAYYFD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.114"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..715)
                     /locus_tag="ACHA25_002556"
     CDS             complement(<1..715)
                     /locus_tag="ACHA25_002556"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8763804.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVL"
     gene            complement(725..2101)
                     /locus_tag="ACHA25_002557"
     CDS             complement(725..2101)
                     /locus_tag="ACHA25_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8763805.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(2221..2868)
                     /locus_tag="ACHA25_002558"
     CDS             complement(2221..2868)
                     /locus_tag="ACHA25_002558"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8763806.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2952..4379)
                     /gene="uxaC"
                     /locus_tag="ACHA25_002559"
     CDS             complement(2952..4379)
                     /gene="uxaC"
                     /locus_tag="ACHA25_002559"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8763807.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(4453..5532)
                     /gene="uxuA"
                     /locus_tag="ACHA25_002560"
     CDS             complement(4453..5532)
                     /gene="uxuA"
                     /locus_tag="ACHA25_002560"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8763808.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(5535..7166)
                     /locus_tag="ACHA25_002561"
     CDS             complement(5535..7166)
                     /locus_tag="ACHA25_002561"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8763809.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7476..>8093
                     /locus_tag="ACHA25_002562"
     CDS             7476..>8093
                     /locus_tag="ACHA25_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8763810.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRW
                     LRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNE"
BASE COUNT         2641 a         1610 c         1079 g         2763 t
ORIGIN      
        1 ataaaacatc agttcttaag ttttcccatg attctttttc tttattgacc aaaataggat
       61 aaaagaaaac tttattttta tttgcagctt ctagatcacc aggagaatca ccaatcatta
      121 atatcttatc caaatcacaa ccattttcaa ttacacttcg aataatatct tctttttttc
      181 ctttatcttg acaacacagt tcatcaacta atgataataa ttcatgtctt tcccattctt
      241 cttgaacagc ttccttatta gctgagctta caataattat cttcccatat tttttcagag
      301 cttttatagt atctctagca cttaaaaagg gtttatctaa tccttttgct tgactaatct
      361 gttgattgac ttcttccgac cattttaaag cttttaataa atcctctgac tgagaatatt
      421 ctatttccct cttcaaagat ggattagaca attcttttgt tagagatacc cagtgcttta
      481 atcgattaat cgatgttaca tcattttttt ctaatgtttt aactaaacct aaaaatctgt
      541 ttattccacg agtctctgaa tataaattaa tattattcca atcctgtagg aatttttctc
      601 tatgatgaac atcaaaaatc tctgcagcaa ttggtccgaa aaagagattg tgtttataag
      661 tcatcgtatc cataacacag ccatctgaat caatacaaaa tatccaatta ttcatatttc
      721 tctcctattt atgtttttgt tttaaaagct cgcaattttc taagacttta tctttagata
      781 atggataaag gaaaaataaa attaatccta caatcagaaa acaaattgat ggaattaatg
      841 ttgaagcgtt atataacttt tctaaaacat ctcccgtttg tacatttgct aaagagtcat
      901 agcctataat agaaagagtc caacctccga cgcctccagc tagagcttga cctatttttc
      961 tagcaaaaga atataccgca tatacggtcc catcttctct ttgatcagac tttacttctt
     1021 gatcatcaat aacatcagta atatttgccc aaataacagt attaaacaca cccaaaccca
     1081 tatagccaac cgaactaaga attaagaata catacatatt actagctttt atcaaaaata
     1141 agatcaaata tactattgct gaaaagaaca ttcctactga tgccagttct ttttttccat
     1201 attttttact taaaaacaaa gttaaaggcg aaacaactat taaaaccaaa ataggattga
     1261 taaaattcat taaaactatt ccctgtgcat cattatagta atcaggaaat acataattat
     1321 tcatactcat aattaataat tgagcaagga gtaagaaaat agccgcacca attatagaaa
     1381 gtaatgccct atttttaact aaccctttta aagattttaa caatgaaact ttgtattgag
     1441 attggtcaat tcttactcgt tcagtagtta gtttataaca tattaaataa caaataagtg
     1501 cacatatcga aaatattccc gcaacaattg taaaagttga tccaccacga actatttgag
     1561 cgcctgaatc atctcttgta taaataaata atggtgttaa tacaccaatg accaaacttg
     1621 ctaaagtagc tccaacattt cggaatgtcg atagcattgt tctttcttct gtaatagaag
     1681 taatagctga tgccattgat ccataaggaa tatttataga tgtgtaaaaa atactccccc
     1741 ataatatata cgtaacatac atgtatacta ccttaacgct catagaagct gaaatcatag
     1801 aagtttgata cattaaaaaa cttgctaaag cgactggacc actcattctt aaaatccacg
     1861 gtttaaactt accctgctta gttgctttcg atctatcaac aatacgcccc atagttacat
     1921 cagtaactgc atcgataaat cttgctacta aaaataatgt tccaacggca gctccgctta
     1981 tccccaatac ttttgtataa aagatcatta aaaaagaact agcaaaaatg aaagtaaaat
     2041 cgtttccaaa atctccaaac atatatccta ttttgtctct tataccaaat tttctttcca
     2101 tacaattctc tcccttctgt tgttaagcgt tttcattctg atgaaagtac gtactatact
     2161 ctgaagcgta aagagtcagt atttattttt tacatttcag agtattattt gttttttctc
     2221 ctaataattc tttatctcat aaaacttatc tatatattta ctagctactc gacttacttc
     2281 ttcaaaatca cctttttcaa cagaagccaa taaattccca cctacaccaa ctgcaacaac
     2341 tcctacttca aaccaatctt ctaagttctc tatacttact ccgccagtag gcattatatt
     2401 tacgtgtggt aatggagctt gataggcttt tacaatggat ggaccaaaag cagaacctgg
     2461 gaatagttta atgatatcta cgccagcagt aagtgcctcc tttatctcag taattgtcat
     2521 acaacctggt aaataaggaa tttgatataa attacaaatc tcagctgttt gtcgatcaaa
     2581 agatggacta acgatatatt ctgccccagc cataattgcg atacgagcag taaacgcatc
     2641 taaaaccgta ccggctccga ttattacatc tttttgatca gcatattttt tagcaatttc
     2701 tttgattatt tcttcagcat taggaactgt aaaagttagt tcaatccctt taattccacc
     2761 cgcaataatt gaatttattg ctttaatggc ctcatcctta tttttcccac gtacaaccgc
     2821 aatgactcca gccgcttcta acttacttaa aatttctact tttttcatat ctatctcctt
     2881 cctttatgga tacatacttt gtctagctgt actaacttac aaagaataaa tttaagcttt
     2941 tatttcaagt attattttct acttccgatt tcaaaaaaat taaaataatt atacgcatta
     3001 cgataacaaa tattctccac tatctgacta agatacattt catcatctgg aattcggcct
     3061 gctttaaccc actcgcttag taattgacat aatactcgac gaaaatactc atggcgagga
     3121 tagcttagaa aacttcgaga atcagtcaac atacctgtaa aattcgacaa caaactttgt
     3181 tgtgccatcg tagtcaactg ttgacgcatt ccttcagctg tatcattgaa ccaccaagca
     3241 catcccaatt ggattttttg aatcataccc ccttggaagc tttgcattgc tgttgctagt
     3301 ggaagccaat cagttggatt taatgaatat aaaattgttt ttggtaagca cccactcttt
     3361 tcagcggtat cgaacaatgc tacaacattt tgtgtcaaac tagcttgatc ccccatcgaa
     3421 tcaccaccga catctactcc aatttgttcc tttaatatac ttgaattatt tcttaaagca
     3481 tttaaatgca tttgcatcgt ccaaccatat cttgtatact cagacatcaa atgtaattga
     3541 atacctgtaa caaattgtgc aatttctgat aaatggaaat ccgtttttcc tgctaaagct
     3601 tttattaaca gattgtcgag tattttttca tctgctattt cataaaaata agtattcaaa
     3661 ccgtgatctg ctaatcgaca gccaatttga tgaaaatgat ccactcgttg gctcaatgct
     3721 tgcttcaacc cattaaagtc tttgattggt atagccgctg tttgactaag ttttaaaata
     3781 tagttcgaaa agcctgatga tttgatactc cagaccttat ctggacggaa agtaggtaat
     3841 accttgaaag aattatcctg tttagaaagc tcagtatgat accacaagtc atctgtagga
     3901 tcatccgttg tacagatggc tttcacatcc atcatctcca acaattcttt cgggcgatag
     3961 gaatccgttt gaattttttc gttagcacgt cgccaaattt ctggtgcatt cttctcagaa
     4021 attgtcaaat caatatcaaa gtaacgttgt aattccaaat gagaccattc aaaaatagga
     4081 ttacctggcg ccttctccac tgtttttaca aaagctaaaa atttttcata atcatcacct
     4141 ggaccactga tcaactcttc ttctatacca ttagctcgca tcaatcgcca tttataatga
     4201 tctccagctc ctttatcatt taaccatacc tgagtcaaat tcttgtattg cttattttct
     4261 agcaaatatg ctgggtctaa atgacaatga tagtcgataa ttggttggtg ttttgcaaat
     4321 ttatgaaaaa gtcttttagc tatttcattc tctaataaaa agttcttatc taaaaacata
     4381 tttaaacact cctttccttc cgttagtttt tagaagagaa aatttttcct ctaaataaac
     4441 tcccttatat aattatttac tcgccccctt ttcaagcgct tcccaaatac catttaaata
     4501 aactgcacct agagctcggt catataatcc ataaccaggt ctaccggttt ctccccaaat
     4561 catccgacca tgatctggac gaatatagcc attaaatcca ttatcgtaca ttgtttttaa
     4621 tatcttatac atatctagag aaccatattc tgatttatgc ggcgcttcgt aaaaatcgag
     4681 tccattgtca cttaaatatt tgatattacg aacatgtgca aaaggaatcc gatcccgttg
     4741 acaaaattca gatagaattt catagacatc attatctggt cgttctgcaa tacttccagt
     4801 acataatgta ataccattag aaggagaatc cactacatta cacaaccaat ctaaatcgtc
     4861 acgattttta acaatacgag gtaatccgaa aatggaatat ggtggatcat ctgggtgaac
     4921 tgccatttta atcccaactt cttcacatgt cgggataatt gctttgagaa aatatgcaaa
     4981 gttctctcgt aatttctctt catcaacttc tttataggct tctaataatt gcttaatttg
     5041 tgataaccga tgaggttccc aacccggcaa ttcaaaatta ttagtagaat tttctacttc
     5101 tctaataatt tcctccggat cagtagggat attttttgaa atataggcta acgtagaaga
     5161 cccatcatct aaacgataat ccaaatccga ttttacccaa tcaaatactg gcataaaatt
     5221 ataacagata acctcaatcc caaattctgc caaattgcga atcgtctgtt tataattctc
     5281 aatgtattga tctcgtgttg gtaaaccaat tttgatatca tcatgaacat taacactctc
     5341 aataactttc aaggtaagtc cattggcttc gacttcatct tttaaagatt taattcgttc
     5401 tctcggccaa acctctccgg ctggtatatc cattaatgtt ccaacaattc ctttcattgc
     5461 tgggatctga cgaatttctt gtagtgagat tttttcttca tttgccccat accatctaaa
     5521 agtcatttcc atagttactt tcctccaact tctgttatat atttattgat tgttttacga
     5581 actgcttgag gacctactag catttcgcaa aagatttttt cagttttctt tcctacccca
     5641 acttgatata aatctaaacc aaagatttct ttattttcta aaatttcctg taccgcacaa
     5701 tgaatctttg aaggatcttg ttcacctaat ttcaatactg ataatttatc ctgcaactct
     5761 accaataacg gatcaggact tggcacaaaa ttttcccctt gatcatcgac tgccagtaaa
     5821 taacgtaacc aagcagcaat agcgatagga ataaatgtta gattttctgc atttccgtct
     5881 ttttcgacat actttttaat tgtttcacca tagcgaatcg ctactttttg agaagtatct
     5941 gttgcaattc gttgtggtgt atctggaata tagggattag gcaaacgttt attaattact
     6001 tcatcaataa attgttttgg atcaataatg cctggatttt caactactgg taaaccttct
     6061 aaataaccta ttcctttgat taaaccaacc aaatcatcat cctgcatctc tgctgaaatt
     6121 gattgatatc ctagcagaca acctgttact gccaaagcag tatgaagtgg attcaagctt
     6181 gtcgtaacct tcatgatgtc tgctttatca accgtctctc tatctgtcat aatcacacca
     6241 gccttttcca atttaggccg gccatttgga aaactatctt caataactaa ataatgaact
     6301 acctcagtat tagtgaaagc agcaatattg gtatgttttt ttgtgtgaat gatatctaaa
     6361 tcctcgagcc ccatcatcaa tagatcttcc tttacattct ctgaaggatt tggcgtaatt
     6421 cgatcaatca tactccaagg aaaagtcact ttttctttat cctttaaata agcaataaat
     6481 ttttcagaaa catatccttc tttttgccaa gcttctgcaa tcgtaaaaat actatcttga
     6541 aaacgttgac cattctgtga gaaattatca gtagaaacca tcgcaatcgg caactctcca
     6601 gcattaaatc tgctatataa caaggatgtt acaatactca tcgtatgttg aggattgcta
     6661 ggtccagatt tgatatcagc tttgactacc gaaaagaatt gccctttaga atcttttaaa
     6721 ctgtaacctt tttcagtaat agtaaacgtt gtaaactgta atttaggatc ttcaaaatat
     6781 ttaaccactc tttgatagtc cgctatattc ttcggattgc aatataaact atcagccgtt
     6841 actgccaaaa gtcttttttt gagttcacca tctccactca taacaatttc caaaatgtca
     6901 ttgttatagt catgataagc tttttccact acttcttcat caaatgtttc acacacaact
     6961 actcctgttt ctaattcatt ttgatctgct aagcattgag caatttctgc atgaaatcct
     7021 cgataaagat ttcctccacc aaaatgaagc catactggat gttgctggcc ttcttgctta
     7081 acttctttga catcaaaaca cggcagtaga atctgcattt cattgaattc ttcgatagaa
     7141 ttttgccaat ttttaatcga aaccataatt aacctcctca aactgatatg ttagttttga
     7201 tacattttat attttaaaac ccattgtaac cgttgtcaat ataaaaaggg aataaacttg
     7261 cttttttcta ccatatcaga atgattgtat tttttattca gaaaaataaa atactccttc
     7321 tttttggact gattttatcg aaacttattt tttctttttt tcatgtattc ttttattttt
     7381 ataactagtg tattacttca aaatttgata ttatatggca tgaacagaat taaaatatca
     7441 gttaaaataa aagttaagga gcattgtgta tgacaattaa aactagtctc caaaatcaag
     7501 cttaccaatc tattcgccga caaattatct atgctgactt agaacctgga aaaaaagtat
     7561 cagaaaaaaa attagaagaa acactttcta ttggtcgtac accaatacgt gaagcactga
     7621 ttcaactccg acaacaaggt cttgttgata caattcctca aagcggaaat tatatctcat
     7681 taattgattt agattcggcc aaaaatgctc gatttgtcag agaacatttg gaacgacaaa
     7741 ttatgttaga gtgttgtgcc aaaatgaata accaaaaaaa acggatttta gaaactataa
     7801 tttcagaaca agaaaaagct gttagtcaaa aaaatgctcg tagttttttt cataatgata
     7861 atttattcca taaagcttgt tttgaaattg cagagcgaga agaagtatgg caatggttag
     7921 aaaataacaa tacacactta gaacgatttc gatggctacg agtaaaaacg gaaggattaa
     7981 agtgggaaaa aattatggaa caacattatc aactatttga ggcattaaca aaacaagatc
     8041 cagaagaagc taactttcta acatcgttgc atcttcattt aatgcttaat gag
//
LOCUS       DAYYFD010000112         7975 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000112 DAYYFD010000000
VERSION     DAYYFD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.115"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..841)
                     /locus_tag="ACHA25_002563"
     CDS             complement(<1..841)
                     /locus_tag="ACHA25_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8763811.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMD"
     gene            complement(1065..3005)
                     /gene="thrS"
                     /locus_tag="ACHA25_002564"
     CDS             complement(1065..3005)
                     /gene="thrS"
                     /locus_tag="ACHA25_002564"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8763812.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3342..3521)
                     /locus_tag="ACHA25_002565"
     CDS             complement(3342..3521)
                     /locus_tag="ACHA25_002565"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8763813.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3568..5391)
                     /locus_tag="ACHA25_002566"
     CDS             complement(3568..5391)
                     /locus_tag="ACHA25_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8763814.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5669..7948
                     /gene="helD"
                     /locus_tag="ACHA25_002567"
     CDS             5669..7948
                     /gene="helD"
                     /locus_tag="ACHA25_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8763815.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2334 a         1703 c         1446 g         2492 t
ORIGIN      
        1 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
       61 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      121 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      181 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      241 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      301 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      361 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      421 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      481 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      541 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      601 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      661 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      721 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
      781 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
      841 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
      901 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
      961 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1021 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1081 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1141 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1201 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1261 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1321 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1381 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1441 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1501 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1561 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1621 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1681 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1741 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     1801 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     1861 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     1921 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     1981 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2041 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2101 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2161 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2221 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2281 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2341 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2401 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2461 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2521 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2581 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2641 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2701 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     2761 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     2821 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     2881 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     2941 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3001 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3061 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3121 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3181 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3241 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3301 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3361 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3421 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3481 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3541 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3601 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3661 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3721 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     3781 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     3841 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     3901 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     3961 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4021 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4081 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4141 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4201 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4261 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4321 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4381 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4441 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4501 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4561 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4621 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4681 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4741 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     4801 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     4861 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     4921 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     4981 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5041 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5101 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5161 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5221 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5281 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5341 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5401 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5461 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5521 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5581 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5641 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5701 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     5761 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     5821 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     5881 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     5941 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6001 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6061 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6121 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6181 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6241 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6301 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6361 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6421 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6481 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6541 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6601 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6661 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6721 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     6781 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     6841 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     6901 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     6961 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7021 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7081 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7141 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7201 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7261 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7321 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7381 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7441 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7501 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7561 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7621 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7681 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7741 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     7801 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     7861 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     7921 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatg
//
LOCUS       DAYYFD010000113         7882 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000113 DAYYFD010000000
VERSION     DAYYFD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.116"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACHA25_002568"
     CDS             complement(220..972)
                     /locus_tag="ACHA25_002568"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8763816.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACHA25_002569"
     CDS             1085..2005
                     /locus_tag="ACHA25_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8763817.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACHA25_002570"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACHA25_002570"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8763818.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACHA25_002571"
     CDS             3311..4789
                     /locus_tag="ACHA25_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763819.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="ACHA25_002572"
     CDS             5066..7126
                     /locus_tag="ACHA25_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8763820.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="ACHA25_002573"
     CDS             7209..7778
                     /locus_tag="ACHA25_002573"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8763821.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         2805 a         1318 c         1505 g         2254 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagaaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcagtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaatca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg cc
//
LOCUS       DAYYFD010000114         7806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000114 DAYYFD010000000
VERSION     DAYYFD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.117"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACHA25_002574"
     CDS             complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACHA25_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8763822.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVTLGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1378..1896)
                     /locus_tag="ACHA25_002575"
     CDS             complement(1378..1896)
                     /locus_tag="ACHA25_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763823.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1912..2238)
                     /locus_tag="ACHA25_002576"
     CDS             complement(1912..2238)
                     /locus_tag="ACHA25_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8763824.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2251..4230)
                     /locus_tag="ACHA25_002577"
     CDS             complement(2251..4230)
                     /locus_tag="ACHA25_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8763825.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4251..4565)
                     /locus_tag="ACHA25_002578"
     CDS             complement(4251..4565)
                     /locus_tag="ACHA25_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8763826.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4733..5026
                     /locus_tag="ACHA25_002579"
     CDS             4733..5026
                     /locus_tag="ACHA25_002579"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8763827.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5104..6216)
                     /locus_tag="ACHA25_002580"
     CDS             complement(5104..6216)
                     /locus_tag="ACHA25_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8763828.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6369..7217
                     /locus_tag="ACHA25_002581"
                     /pseudo
     CDS             6369..7217
                     /locus_tag="ACHA25_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7570..>7806)
                     /locus_tag="ACHA25_002582"
     CDS             complement(7570..>7806)
                     /locus_tag="ACHA25_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8763829.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1553 c         1262 g         2496 t
ORIGIN      
        1 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
       61 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
      121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
      181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
      241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
      301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
      361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
      421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
      481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
      541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
      601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
      661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgtaacta
      721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
      781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
      841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
      901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
      961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
     1021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
     1081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
     1141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
     1201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
     1261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
     1321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
     1381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
     1441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
     1501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
     1561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
     1621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
     1681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
     1741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
     1801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
     1861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
     1921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
     1981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
     2041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
     2101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
     2161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
     2221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
     2281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
     2341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
     2401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
     2461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
     2521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
     2581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
     2641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
     2701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
     2761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
     2821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
     2881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
     2941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
     3001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
     3061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
     3121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
     3181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
     3241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
     3301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
     3361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
     3421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
     3481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
     3541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
     3601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
     3661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
     3721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
     3781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
     3841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
     3901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
     3961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
     4021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
     4081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
     4141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
     4201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
     4261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
     4321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
     4381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
     4441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
     4501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
     4561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
     4621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
     4681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
     4741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
     4801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
     4861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
     4921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
     4981 gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg
     5041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
     5101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
     5161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
     5221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
     5281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
     5341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
     5401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
     5461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
     5521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
     5581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
     5641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
     5701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
     5761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
     5821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
     5881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
     5941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
     6001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
     6061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
     6121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg
     6181 tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg
     6241 gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa
     6301 aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga
     6361 tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga
     6421 ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc
     6481 tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga
     6541 aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc
     6601 atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc
     6661 attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac
     6721 ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt
     6781 aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt
     6841 cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt
     6901 tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat
     6961 ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa
     7021 gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt
     7081 ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag
     7141 gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt
     7201 tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta
     7261 tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact
     7321 gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact
     7381 acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt
     7441 tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg
     7501 tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt
     7561 tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac
     7621 acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt
     7681 atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt
     7741 ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt
     7801 cgcatc
//
LOCUS       DAYYFD010000115         7631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000115 DAYYFD010000000
VERSION     DAYYFD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.118"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHA25_002583"
     CDS             <1..107
                     /locus_tag="ACHA25_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8763830.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHA25_002584"
     CDS             complement(176..904)
                     /locus_tag="ACHA25_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8763831.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHA25_002585"
     CDS             complement(917..2197)
                     /locus_tag="ACHA25_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8763832.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHA25_002586"
     CDS             complement(2338..2940)
                     /locus_tag="ACHA25_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8763833.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLE
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHA25_002587"
     CDS             3185..3391
                     /locus_tag="ACHA25_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763834.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHA25_002588"
     CDS             3703..4422
                     /locus_tag="ACHA25_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8763835.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHA25_002589"
     CDS             complement(4503..5186)
                     /locus_tag="ACHA25_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8763836.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHA25_002590"
     CDS             5405..7591
                     /locus_tag="ACHA25_002590"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8763837.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2741 a         1289 c         1437 g         2164 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattccag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggttg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa a
//
LOCUS       DAYYFD010000116         7599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000116 DAYYFD010000000
VERSION     DAYYFD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.119"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACHA25_002591"
     CDS             complement(136..426)
                     /locus_tag="ACHA25_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763838.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACHA25_002592"
     CDS             complement(544..804)
                     /locus_tag="ACHA25_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763839.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACHA25_002593"
     CDS             complement(941..1354)
                     /locus_tag="ACHA25_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8763840.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="ACHA25_002594"
     CDS             complement(1810..2295)
                     /locus_tag="ACHA25_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763841.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="ACHA25_002595"
     CDS             complement(2434..2649)
                     /locus_tag="ACHA25_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8763842.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="ACHA25_002596"
     CDS             2873..3673
                     /locus_tag="ACHA25_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8763843.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="ACHA25_002597"
     CDS             3692..5560
                     /locus_tag="ACHA25_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8763844.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="ACHA25_002598"
     CDS             5553..6044
                     /locus_tag="ACHA25_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8763845.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /locus_tag="ACHA25_002599"
     CDS             6032..6586
                     /locus_tag="ACHA25_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8763846.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="ACHA25_002600"
     CDS             6604..7599
                     /locus_tag="ACHA25_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8763847.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAYYFD010000117         7349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000117 DAYYFD010000000
VERSION     DAYYFD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.120"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..682
                     /locus_tag="ACHA25_002601"
     CDS             <1..682
                     /locus_tag="ACHA25_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763848.1"
                     /translation="VLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVYKGDF
                     HFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNL
                     KYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYY
                     WEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKIYKE
                     IIEQYFPK"
     gene            865..3540
                     /locus_tag="ACHA25_002602"
     CDS             865..3540
                     /locus_tag="ACHA25_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8763849.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3722..3961
                     /locus_tag="ACHA25_002603"
     CDS             3722..3961
                     /locus_tag="ACHA25_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763850.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3977..5350
                     /locus_tag="ACHA25_002604"
     CDS             3977..5350
                     /locus_tag="ACHA25_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8763851.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5354..6988
                     /locus_tag="ACHA25_002605"
     CDS             5354..6988
                     /locus_tag="ACHA25_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8763852.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7151..>7349
                     /locus_tag="ACHA25_002606"
     CDS             7151..>7349
                     /locus_tag="ACHA25_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763853.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2622 a         1142 c         1458 g         2127 t
ORIGIN      
        1 tgtcttatta ttacatggaa gagttattaa cttagttaat cgaaaaagta cgactaaatt
       61 tgcaaactca ttctggtttt taaaaaatat taataaaaca gtggaaaaga atggagttta
      121 taaaggtgat tttcattttc aaaatgctga gactattgaa cgagaacatc attatttcga
      181 tttattagtg aatacagtgg tagaattagc gaaatgggca aagtcaaacg aaacttatag
      241 tcaagtactt aaaagaatta tgagtcaaga aacttcattt atctatgagt taagcaagtc
      301 aaatttgaaa tatacttata atgaagatga acttgaaatt ttagataatc cagaaaattc
      361 acaatggatt aaagagttga agagagaaga ctttattgag acactaagaa ttctaaaatc
      421 gttatttgat ttgaaattag ccaaagctac catcatggga attgaaagag attcatatta
      481 ttgggaggaa attggactat atgctcccca gattgaaggt gaagataaag agaaaaggat
      541 agacaacatt attaactata ttaatagttt aagtgaacct ttaaaatgta gaggacttga
      601 ttttatagga acattaggat ggacagaaac atactatgag aaaatatata aagaaataat
      661 tgaacaatat tttcctaaat aatttaataa ggattgattt atcgtaaaag aaaaaagttg
      721 tgatttccaa aatcacaact tttttctttt gtcaagattt tgagtgggat atatttttgt
      781 agaactgaag atcatgaaat tatgagaggt tggaggtaat gtagtcatac aaataaaaat
      841 tttttagaaa gtgagtggta attaatgaaa atgaatcaca cttcaattca gaagcattta
      901 caactgttaa atacactggg gatttcttat gttaatttga gaactttttc tacattaaac
      961 aagaaaaccg tttctcatat tttttctact gcagattctc aagcaattat taactattta
     1021 gaaaaacaga aattgtctga aggtacagta aacataactt ataatcctat taaacaagag
     1081 gtgattacac aaaaaaagca ttctgtgaga gacaatgata ttgaaaaaat tcggtttgta
     1141 tttattgata ttgaccctaa aaggaaagag ggaagtgcaa cagatagcga aaagaaaaag
     1201 gcagaaaatg ttatggaaca agtagaacgt tatttgaaag aaaagagaat agaatcattt
     1261 gtaaaagttg atagtgggaa tggctaccat atttttcttc ccataaatga acaaccaaat
     1321 aatcacgaga cgattcttac tattcagaat tttcttcaat tacttcaccg aagatttgga
     1381 gtagaagatg aagttgatat tgatacttct gtttataatc cttcaagatt atgcaaatta
     1441 gttggtacac cagcggtcaa aggaaagtcc acaaaagagc gtccgcatcg tttatctagt
     1501 ttcctttcta ttcccgataa catagataga agtaattgtt ttaaaattct tgaacagata
     1561 gttgaagaaa attccatgat gtcaacctct agagtaaaaa gccatccatc ttttagagaa
     1621 gtgttacctt ttattcaagc agactataaa caatggttag ctcactatcc tgaattaact
     1681 tataccataa aagagggcga tcataaagga gttaccttac ttatatttga cgaatgtcca
     1741 ttaagaaaac acacgaataa ttctaatggt tcttgtttgt ctgttacggc tgataataag
     1801 attcgatttc attgtctgca tgcttcggat aaaaataaag atattcaaga ctttgtaaaa
     1861 aaatatccgt tgccaaaacc tgtaaatcct tctgtagaat cattagaatc caatcaagaa
     1921 tattattttg accagtttaa actgaatcaa gaaggagtat ttttggtgaa agaaggtcaa
     1981 gaagaaaaag ttgcttgtcc gatgtttatt aagcaaatac gcaaaaatat cgaacaaaaa
     2041 gtcattacac ttgttcttgc ctattatgtc gatcattgtt gggatgaaat agaaatagaa
     2101 ggtggatgtt tacaaactaa taattttaaa caattagcaa aacatggctt aatcattaaa
     2161 gctagaagag aagctgaagt cgttgacttt ttacagattc aacgggagaa tgctccaatc
     2221 ttttttgaac atgaatctct tggctggaaa aaagaaaatg aagcaatgat ttttcagtta
     2281 caagattctt ttggacgtga aattccttct gtattaacta aaaactcatg ctaccaatta
     2341 acaactaatg gtacagaagc ggaatttgac gcattggttc ataagtatgt tttaggaacg
     2401 aacttagaac ttgcttggtc gattggtatt gcttctattg tgttaggcta tttaaacgtt
     2461 acgaaggcta atactttttt gagtttacta attaacctac agggcaagag cacaacgggt
     2521 aaaaccacta cgttacactt tattgcctct ttatttggaa atccaactac ggttcttaga
     2581 aatatgaatg ctaccaataa agccattata aagctagcat caaacaatca gggaggtgtt
     2641 ccactaatac tagatgagtt aggagcagca actcaaaata actttagctc gttgatctat
     2701 caattagctt caggagaaga aaggctaaga ttagattcta attgtcagct tatgccacaa
     2761 gagacttttt ctgttttgac ccttacctct tctgaagagc gtttggaaaa ttatttcaat
     2821 gagaaattaa ctggtttgaa agttcgatat atcgaatttt ctgatatcaa gtttacaaag
     2881 gatgcgaatc acgcagagaa agttaaaagt ctttcttcta atacttttgg ttggggtata
     2941 aagttatttc ttttgaaact ctttgaacaa gggattacaa aaattgaagc aacttttgtc
     3001 gaagctcaaa aaaaactatg ggctgtttta cctgaacatt ctttgaagca acgcattggt
     3061 aataatctag ctttgattta tagtggagcc ctcctaattc aagatatact gggctatcct
     3121 ttacgattag agcatataca gacggtgctt attaattcgt acgaacagtt aattgtagat
     3181 actagtttag gaagtgagca aacggtattt gaccgagtga aaacattgtt ggtgatgaat
     3241 agccataaat ttttaacaaa cagtgacaac aaacatacgc ccagtacgat ttggggaaaa
     3301 gtatcaattc aaaaaaatgg ggatattaag gtaaatattt ttcctatccc gtttgaggac
     3361 ttgttaaggc gagaatttca agtaaaggat ctaacctatc tttttaggca attgcttgat
     3421 tcgggtaata tgtgtgcaga aaaagggaga agaacgaaac gtattcacat gaatcgtcaa
     3481 gttcttccaa catacgagat tctattacct tcttcactca aatcgtattt tcgattgtaa
     3541 tttagaataa accaaagtat gaaagtgagg caccatgtta tggaaatatt tcttattgat
     3601 ttagcaaaac aactgattac ttatctgtat gatagcgagc aaacccaaac ggaagtaaat
     3661 gaagcttgat ctatgcttca tttacttcat tcaatcaaac atgaaagaaa ggaaaattct
     3721 aatgactaaa aataaaatga cactaaaagc agaggttctt ctgtatattc aagaacattt
     3781 ttcaaatcaa gcctttttta caaaaccaat ttacttggat ttcgaaataa gaggggtatc
     3841 tgctggatct atcgggggta ctcttcaagc gttgaagaac gaaggatatt tagaaaatca
     3901 ctttgtgcaa cgttcgttca atagaagagt cgtgaaaaaa tggtatttag ttcattcata
     3961 gagaggggaa tcaagcatgc gtgtggcttg ttatgatcga acgtctgttt atgaagaagc
     4021 gaaaattagt aatgacgctc aatatgatgc agtgaaagag tatgttttag agaaatatga
     4081 acaaattccc caacgatttt cagacaaagg agaatcaggg gaaaaagcgg agcgtccagc
     4141 ttttcgacag cttagagctt gggtttgtgc gtcagaagaa gagggggtga tctatgtttg
     4201 gcgttatgat cgattggcac gtcaagccaa aatagcctta gaatttattg agttgtgcca
     4261 acaacatcag gttcaagtgt attcgatttc tgaacctctt cctgcttctt tctcacacgc
     4321 gtcaacgaat aaactatttg tgcaattatt gttcactttt gcagaacaac aacgaaacgt
     4381 cattattgag aatgttcgtg ccggactttc ctataaaaag caacaacggc aatatttgtc
     4441 ttctgcggta ccatatgggt atcgtttagt gaatgggaaa atccaacaag aagtacacga
     4501 agcaaggatt gttcgctata tttttcaact ttatttgacc aaaaaatatg gctacaaaaa
     4561 gctttgtcaa cgattgacgc aacaaaagct tttcttccgc gaacgtcctt ttcagcccta
     4621 tcatatttat agtatcctaa aaaatccatt gtactatggc gaaatcaaag gaggatcttt
     4681 ggggaagtat ctaggaacct ttgaaccgat tctctcgaaa gcaacctttt tacaagccca
     4741 agagattcgc caatcaaggt gtacagctaa aaaagatacg tatctgtact tattacgtca
     4801 aaaaattcgg tgtcccttct gtggtcgtca tctgagttcg aagtatcaat ggaatacaaa
     4861 gaagacgaag acgcttcatt attatcattg taccgaccga agctgccaag gaatttttct
     4921 tcgggcagaa agggttgagt cagaagtatt ggaaaagata caggcttttt ttcaatacga
     4981 aggcgtctat caagccttga ttgcccaatt aacgcaacaa ttagccgaaa agcgaaaaga
     5041 tgaaaaacgc aaagtaatcc aacaaaagaa acaagaagca caatggatta aggcatttga
     5101 agagggcaat tgttcactgg aagaactcaa gcaacaactg caagctcttc aaaagccgat
     5161 gtctacttct tcgtttcttt cactccaaca gtgtgaagca aaagtgaaag aatggcttca
     5221 actaaaagac ctctctagtc gacaattatt atgtgactat gtttgtcgga ttgaggtaac
     5281 aaaggataaa acagtttgtg ggatttactt ccgacaactg acacaaaata ttttagaaag
     5341 gaacgaatga tctatggaaa tgatacaaac caaacgagtc gcattgtata ttcgcgtgag
     5401 tacacaagaa caagctttag aaggacatag tattcaagca caagaacaaa atggacgaca
     5461 gtttgccgaa cggatgggct atgaagtggt agaagtttat gccgatgaag gaatcagtgg
     5521 gaaatccaca aaaaatcggt tcgcctatca acgaatgatg aacgatgctc ggaaccatcg
     5581 ttttgatcta ctagtgattt ggaaattgac ccgtctcgga cgcaatatgt tagatgtctt
     5641 acaaacagtc gaggaattat tgcgatatga cgtaggatta cattcgatct cggaacaatt
     5701 tgacgtgacc acttcgacag gtaaattgat gttacaactc cttgggagtt tcggtgaatt
     5761 tgaacgcaac caaattgcgg agaatgttca aatgtcaatg caaagcttag tgaaagataa
     5821 aaagcgttat gcaggaggcc gacgtttagg ctatgtcagt ggtattgaca cgaatggtca
     5881 aaaacaattg gtcattgaac cagaagaggc gaaaattgtt cgtctgatct atgcaaaata
     5941 tttaggcgga gcagggtatc gagcgattgc caattttttg aatcgtcaag gatatcaaac
     6001 agtcaaaaag aatgcttttt ctaccacggc agtcaaagat atcttacgca ataaaattta
     6061 tgcagggtat ttagaatatg cgcgctacat caattgggaa accaaacgac ggaaggggaa
     6121 gaatccgaga ccgattttag tgaaaggaac ccatgaaccg attatttcgg aagaagatta
     6181 tcaaaaagta caagaacgcc ttgctttaga aggaaaacaa cccaaatgga accatacagg
     6241 tgaaaatgta ttgacaggct tgttacgttg tccagaatgt ggcgcaccga tggcagcgag
     6301 taatgtcaca aataccctaa aggatggcac aaagaaacgg attcgatatt attcttgttc
     6361 agtctttcgg aataaaggag caagtgtgtg tcatgccaat tcgattcgtg ccgaacaagc
     6421 ggaaacattt gtcgccgacc gattaaagga gatcgttcag ctaccagaag ttcttccccg
     6481 attggtggca gcgttaaatg aagaaatagt gcgacaaagc caaccattgg aacaagaact
     6541 cgtggtgtta ctggaacgaa aggaagaatt gaagactaaa attgaaaaat gggaagctgc
     6601 tttggaagat agtcctgaat tgtttccgat gttaaaagac cgcttagacg aattgactga
     6661 aaaacgacga cagttgcaca ttcgagaaaa tgaaatctta gggatctttc aacaaggaga
     6721 gccgattcaa gtcaaagatg tccaacggat attgactagt ttggatcgtt ttcttgccca
     6781 gagtgagaag aagcaaatca aagccttgta tcgtaccttt atagagaaaa taacctttga
     6841 tcctaaccat aaggagatgt tggaaattac gatgaaattc acgcatgccg tggtacaaca
     6901 attgaatgag caatatcaaa tagccgtatc caaaacaaag gatacggcta tttttgtgtt
     6961 aaaaacaccc tttacgttaa cgatctagtc taggttatat caaacaacgt tagaggggcg
     7021 ctaacgcttc tctagcgatt tgaaatcgtt agagaagtag ttagggaggt ccttatcata
     7081 aatagggata tttattaatg aatatttgaa atataattgt atataagaat taaaaagagt
     7141 ggggatagat atgaaaaaga tgggtaagtg tattttatgt gaaaaataca ctgaacttac
     7201 taaagaacat gtaccaccta aaaaatcagg gaatactgga ggtaaaaaga ccagaactgg
     7261 taatttagat gatttcctta agagcgattt tacaaaaggt gattttccga aggggattaa
     7321 aaggaaacct caaggaaatg tttattata
//
LOCUS       DAYYFD010000118         7343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000118 DAYYFD010000000
VERSION     DAYYFD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.121"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(303..1028)
                     /locus_tag="ACHA25_002607"
     CDS             complement(303..1028)
                     /locus_tag="ACHA25_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763854.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1182..1508)
                     /locus_tag="ACHA25_002608"
     CDS             complement(1182..1508)
                     /locus_tag="ACHA25_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763855.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1545..1910)
                     /locus_tag="ACHA25_002609"
     CDS             complement(1545..1910)
                     /locus_tag="ACHA25_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8763856.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1947..2162)
                     /locus_tag="ACHA25_002610"
     CDS             complement(1947..2162)
                     /locus_tag="ACHA25_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8763857.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2291..2626
                     /locus_tag="ACHA25_002611"
     CDS             2291..2626
                     /locus_tag="ACHA25_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8763858.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2702..4699)
                     /locus_tag="ACHA25_002612"
     CDS             complement(2702..4699)
                     /locus_tag="ACHA25_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763859.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4797..5072)
                     /locus_tag="ACHA25_002613"
     CDS             complement(4797..5072)
                     /locus_tag="ACHA25_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763860.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5304..6038
                     /locus_tag="ACHA25_002614"
     CDS             5304..6038
                     /locus_tag="ACHA25_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8763861.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6077..7219
                     /locus_tag="ACHA25_002615"
     CDS             6077..7219
                     /locus_tag="ACHA25_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8763862.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2485 a         1351 c         1039 g         2468 t
ORIGIN      
        1 gatacatatg aaacaattac atatgatttt ttcataaaaa aacagtactg tctagtagta
       61 taaaatatac aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa
      121 acacttatat atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata
      181 tagtcgggct ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga
      241 tgtataagta cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat
      301 cattattcaa taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat
      361 accacagaat aatctttttt cccattttca agtaaccgaa gtggaatatc tttaccaact
      421 ttctctgtca ctgcattact gatacctgtt agattagcta ataaatctga taaagattga
      481 gcatctgtag aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagt
      541 ttccaagtag gtggtatagc tgtgtatgtt tctacaaatg aattaagttt ttcattaagt
      601 gacttctctt ttatatttcc atctaaaaac tctgtatata tggcttgagc tatactaaaa
      661 caaagctggc cttgttctaa taattttaca tcattagtta gtaaagctgt gttcacgtta
      721 tcaatcgctt caagaagagt atctttggtg tccctaacag ctttaggtgt gccttctgga
      781 atttccctac ttgatacctc gtccatttta tcttccataa accccttaaa atcatatctt
      841 tctcccaaat ccatagaagt ttttgagaaa gctttcattt ctgagtctat ttcttcaata
      901 atatttaaca tgtctgatac atattgttta ggctgattcg tttttaatct atatgtggta
      961 aatatctcaa ttcctccaat tatcaataaa attgccaaac aaatccataa taattttttc
     1021 tttttcattt ttcctcctat acactgctgt tataacagtg actaatgtaa agattttccc
     1081 agcaatcatc agactattat ttacagggaa tcaaaaaaac tccttagtaa gcttacacct
     1141 ttaggagttc ctgtttgaac ggtatgcata atacatttaa atcaatttaa cattaacacc
     1201 tcagtatatg gtatctctac atatacacgg tctttttttg gatagtactc gacatttata
     1261 ttgatagcac ttccattgtc taacgtagct ttagcccggt acgtgttatc accattatca
     1321 tgagtgatcg tcacatcatc acatgtccga tttaagttat actgttcttg taaaatctga
     1381 gttaccaatg gtacagatga cttctctagt ccagatgaac ccgtcattct tgaaaaaatt
     1441 atacctataa tagccaatac aacgatacct ataatgacaa acttcttatt catatttact
     1501 ttattcaact ttttatctcc tatctccaaa atgattttct ctcatcagta actcattgac
     1561 atccccaata gtacaccaaa gatggctcca gctacccctg ctatcatcaa aatcgtaccc
     1621 atttgttcat ctttctctcg atataagtaa cccataataa cagcaacagc accaaatacc
     1681 acaggaataa atgctaatga tactaccata gaaatccaac caataatcaa atacagcttc
     1741 cctgtctctt gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt
     1801 ttctttactg tgtctgttcc ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg
     1861 caatacttac tttcatttgt tatttcttta ccacattttc tacaatacat gtcttctcct
     1921 cctaaaatca ttcaattgct tattagctaa ctgtttactt tattaaatac atcagaaaac
     1981 agctcattta ttggtacact taatatctca gaaagcattt tcatttgata gggtttagga
     2041 atcgttctcc ctgtttgcca cgaacttaca cattgctgtg taacattcat ttttttggct
     2101 aactcagttt gggaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc
     2161 atgattctcc cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat
     2221 atataaatac ttcaaatgtc attacaaata caacaacttg ttgtactcta ctgagaaggg
     2281 gggtgtatta atgaaacaga agcaatatgc aaaggtcatt agagaaaagc gaaaagcact
     2341 cgggtggaca caaaaagaat tagctaaaaa aatcttctct acacaacagg cagtagcaag
     2401 atgggaaaat tctgtaacag aacctaacct cgatagctta acagctcttt ctagagccct
     2461 aggaacccct gtaagccatt ttctagacaa tgttgacata gattatgagg aagagttttt
     2521 ggctctctat cgctctctta gtactgaaga tgcaattcga acaattgact atatgaagct
     2581 attaaaaagg caagaaaatg aacgcaatca gctattgaaa gattgaaaaa taaataaccc
     2641 accgaacttt aacggtgggt gtattgagat gaaataagat taagagctat ggataacctg
     2701 tctagtttaa tataattggg tctcttctat tccttactct ccgtccatca tctcttgtta
     2761 cacttgtaaa ttgataaata ggaactaatt caactcctga taatatcaat tcttttatta
     2821 cttcatctat ccctgcccct ttcgctagat atactaaaaa actactgctt gctttcacat
     2881 tgtatagttt cttaatatcc ttagaagctc gttcaagagt gaatccttgt ttaacaaagt
     2941 agcctctgtt ttctattaaa gcatttttca cgctgttatc cctgacattt cgtaggcgtt
     3001 cacaaacagt gttaataagt tctttttctg tcattagata tgtcccttgc cacgataagc
     3061 cctgtaaaac ttcatgtgct tcatctaaaa tcaattgttt ttccttgcta agttctgatc
     3121 gggaattttg aggaaatact cttttagcct ctttttcgtt aaaacatatc ttaacccatt
     3181 cataagaaaa atattgatat gtaaaatgaa atttttccag aagtggaagg atatcttcat
     3241 tcaatttttg aatcacagaa gttgcattaa aaacgtctaa tcgatagaca ttgcttctct
     3301 tatgtttaaa gtttcctaac gctttctctt cctttaacct atttaggtat tcgagcatct
     3361 tcaaaggaac atcttcatca tctaattttt ttatcaatcc tataaaagtc ataagtgata
     3421 gtaacttatt acatttatct atgtcataag ttttaccata gtaagaactc atttttttag
     3481 ctaattgttc ccccgacaaa atacaagtca cactttcctt actatcatca aaaaaattaa
     3541 catcagaaag caagacatca tagacacgtc gcatttcctc tagataattc cttattgtcc
     3601 tgaaaagttt gtctttcatt gattcaagag tgtcaaaaag tacttttgat tcctctttca
     3661 attttcgaaa atgtgtagga tacaagtcta ggttctctaa tagccaatta aatgtttcta
     3721 cccgactgtc tttctttaaa attagcatca caactccaat aaaattttca ctgatatcac
     3781 aattactgga atgacaataa aaaatggaat gcccagtttt actggtaaaa atactagcag
     3841 agggattatg atcgttgtga aacagacatc ttaagttttt acttgactta agtaatttat
     3901 tcatatcaat ggttaacaat ctttcataaa tctcattaaa agacatgtcg gaggaatcaa
     3961 acaggcagtc tttaaaccac tgtttcattt cttctttgtc gtcatttttt atcaacttaa
     4021 caaaatcggt ttcattgact agttcatact ctctccctga atcactttga caatttttag
     4081 aacatgtcat ggtaaaatca atcggaagct cagtaatatc taacatgttg tcttcattaa
     4141 ttattatcac tgcatctgac ttatatcctc caaaaaacat tctattcgaa gcatttacat
     4201 tgtcatcaca cccggggatg ttctttttca agtattcgta aacagcaacc attttaccat
     4261 agtcagtaat tgcttctttt aatagaaaga caatcttgta tttttctaga ttttcagagt
     4321 accgaatact tttttgtatc gcaaacgcag aattatgaac taaattgata gataaaacct
     4381 tttctatgct taagtatcta ctatcatctt tactcaaatc tactaccttc cccttctcat
     4441 ctttaacagt gttatcgaca tccagaataa ttactctggt ttccacaaaa ctctccttgt
     4501 cattgccttt attctttgtc ttgcttggaa taaatgattg tgatgaattt agtatctcta
     4561 caaactcatc tcttgtaact tctctatagt tatctaaaca cctatctctt acatcagttt
     4621 tttctttttt tgtaactggc ttctctaata atttttcttt atctacataa attggaatat
     4681 taatcattga tgtttccatt tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca
     4741 tacagataat aaaaacaaac tctttattac tatatgtttt agtcttattt gtttactcaa
     4801 cctttatacc gttccatgaa actatcaata tcagattttt taaatctaat gaccgtatca
     4861 atttttataa caggtaaccc gtattgagaa acatattttc taatcgttcc tactgataca
     4921 cctagatatt cacttgcctg ctgatagtcc atatactcta gagtgactcc agaaataatg
     4981 tcttctagca taggctttag cactgtaatt atctctttca cttgttgttc aacttgtata
     5041 gaaacaatgc tatcaatgat ttgtagagac atatttcccc ctcctttcgt gattgcttga
     5101 acaaattttc taagaattat cttcgtattt tttgctcaaa ataaaagtat tttgttatta
     5161 accgattaat caaacaaaaa atgattacca gttcatcgac atacacataa tacctcgata
     5221 aaaaaggggt gtcaatagtt ttgtaaaatt tatttacaac accccttttt tgaggtatac
     5281 taattttagg aggtgtggtc aatatgataa aacgtccacc aataaattat ttagaacgaa
     5341 aaaagatact cggaacaaaa ataaaagcta taagaaagag taaaaaatta acacaaccag
     5401 catttggtct tatgattaac aacggtcaat taattgacaa aaagactatc tacgaatggg
     5461 agaagggaac ctatttacca ataccagagc gattatcccg aattgctgat ttgggaaata
     5521 tgagtataga ggagttagtt tgtggcaatg tagaagaata tattttggga attattcttt
     5581 atagagatag cattgtacta gatggtataa catttccaga taaaaacctg ttccagcatc
     5641 ttcgtcaaca gttcccacca gtacactcta atttagatac atggctggat agatattcca
     5701 aattagaacc agagatgcaa gaatttattg ctaacaagac ttgtaataaa gttaaaaatg
     5761 aaaaaattag tcttttcaat atcttaaaaa tagaagaatt attcatcaat gctattgtag
     5821 aagaatttga caataacatt cttttcctca ctagcagtat agaagaacta ctagaaagaa
     5881 tggtagatga atggctacct attcaattga aagacatgtc ttatcccgaa gaagctgtga
     5941 gagaaattac tgataatata aacaagctag aacaaacgat ttcatcaatt gggaaaaaat
     6001 acactaagaa aaaaatgaag ggaggtgata ccatctagtg gtaagtactc cttgtaaact
     6061 aagaggagat gatttcatgg caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc
     6121 gtggatgtat aaaggacaca ttgccaccga tccacgaact ggtgaaaaaa taaatactac
     6181 gagaagaggt tttagtagca aacaagaagc tcaaaaagat tatgacgagt acagacatca
     6241 aatattatac ggtgttaaaa agaaagcacc tgatatgaca tttgaagacc tgtataatga
     6301 atggataact catcagcgta ccagtgttaa agcttcaaca atcgcaatct cagtaagata
     6361 cgcaaataat caaatacttc cagcatttgg taagcttaaa ctatcgaata tctctgtccc
     6421 atattgtcaa aaagtggtag atgaatggca cagtaaatat gaaagttacg attatatgag
     6481 aaaacagact gcacaaattt tacgctacgg ggttgcaatg caatatattg acaataatcc
     6541 tatggaaaaa acacttcttc cacgaaaaaa agagtatgag aaaaatcgta aattttactc
     6601 aaaagaggag cttaataatt tactggatgc ttttaaagat tttggtaaca tgaaacaata
     6661 cgccttcttc agattattgg catacacagg tatgagaaag agtgaagtac tcgctttaca
     6721 gtggaaagac atagacacgt ttaataaaga actacatgtc aataaaactc ttgctgtaga
     6781 tgaatttggt aaagttataa ttcaatctcc gaaaacaaga gcttctagac gagttatctc
     6841 tttagatact gagactcttt ctatcctcaa caactggaaa ttacagcaaa aagaagagta
     6901 tttaaaactt ggatacaata ctagctctaa agagcaacat gtttttacaa cagttaaaaa
     6961 cactctatac attccaaaca ctgttaatga ttggttaaga tatattttga aaaaatataa
     7021 cttgcctcgt attacacctc atgggtttag acatacacat gctagtttat tattagaagc
     7081 aggagaatct gtcaaggtag ttcaacaacg attagggcat gaaaatagta aagtgacatt
     7141 agacatctat gcacatatta caaataatgc acctaagaaa acaggtcaag actttgctga
     7201 tatgatggct catcagtaaa agagctggtc aaaatctggt catttctagt atacaggcaa
     7261 aacaaaaaag ctagaaccct tatatatcaa gggttctagc tcctataaca ggtttaccca
     7321 acactgcctt ccaatgactc ttt
//
LOCUS       DAYYFD010000119         7264 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000119 DAYYFD010000000
VERSION     DAYYFD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.122"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..221)
                     /locus_tag="ACHA25_002616"
     CDS             complement(24..221)
                     /locus_tag="ACHA25_002616"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8763863.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(367..1245)
                     /locus_tag="ACHA25_002617"
     CDS             complement(367..1245)
                     /locus_tag="ACHA25_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8763864.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1284..2402)
                     /locus_tag="ACHA25_002618"
     CDS             complement(1284..2402)
                     /locus_tag="ACHA25_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8763865.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2399..4498)
                     /locus_tag="ACHA25_002619"
     CDS             complement(2399..4498)
                     /locus_tag="ACHA25_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8763866.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4549..5370)
                     /locus_tag="ACHA25_002620"
     CDS             complement(4549..5370)
                     /locus_tag="ACHA25_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8763867.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5367..6176)
                     /locus_tag="ACHA25_002621"
     CDS             complement(5367..6176)
                     /locus_tag="ACHA25_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8763868.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6197..6691)
                     /locus_tag="ACHA25_002622"
     CDS             complement(6197..6691)
                     /locus_tag="ACHA25_002622"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8763869.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6703..7203)
                     /locus_tag="ACHA25_002623"
     CDS             complement(6703..7203)
                     /locus_tag="ACHA25_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8763870.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
BASE COUNT         2207 a         1680 c         1215 g         2162 t
ORIGIN      
        1 ttttatacgg ctgaaaaaat ctatcaccaa aaaaattcaa acattaagcc aaggattatg
       61 aagtcaacat ttcgaaaaat gaacatacat tggtaagcaa ctataactat taccacagac
      121 ataaagtaac acaatatatt actatttaag tgtacgccac cagtgaatat cctcaaggta
      181 tacagcgcac aacaaaaaga aaaaaggaaa cgcagaagca tttcctttca gtgatttaat
      241 gaaaacaaca cgatttgact tagagccttt ttatttgaat tcattttcgc tttatccaac
      301 ttcatttgat catttgatta ctcggttaaa tacttttgac atgagtatca atgaaacatt
      361 cattaattat ggttgcttat ttcttaccgc ttttctggcc aatgctaatc cgccgattat
      421 tccggcatta tcacctaatt cgggcgtaat aatgtagcta tcaattggtg gtatttcaac
      481 atatccattt ataagctcaa caaatttatc ctgaactttt ttcttcatgt gttcttgttt
      541 cattacacca ccgccaaaaa taattctatc tggcgaaaac atcagtgttg tattgtatgc
      601 acattgggca aggtaaaagg cttcaatctg ccaaaacgaa tcgtcagcca ataaattctg
      661 gccttttaca cccaaccgtt tttcaatcgc tggtcctgct gccatcccct ctaaacaatc
      721 ttggtggaag ggacagtttc ctgaaaaatt gtcctttgga tgtcgcttga tcaacatatg
      781 ccccatttcc ggatgactga acccttccac gaaagtccca ttgaacaagg caccacctcc
      841 gatacctgtt cccacagtga agtatgcaac acctgctaac cctttgccag aaccagcaac
      901 atattcacca tagcaagccg cattgacatc tgttgtccat tcaatcggaa tattgaaata
      961 ttgctttagc gttcctacaa aattgaaatt ttgccaagca agttttggtg ttgatgtgat
     1021 atgaccgaaa ctcgttgatt tgcattgaat atcaattgga ccaaagcagc cgaccccgat
     1081 gcctgccaat tgttccttaa aaggtttaaa aaaatcaatg acctgcttca ttgtttcaac
     1141 tggagttgtt gtgggaaaac tgactctttc caaaatcgtt aattcctcag taccgactgc
     1201 acagacaaat ttagtccctc cagcttcaat tgccccatat atcatcatag gttcctccct
     1261 tcgaatatgt gaaaaacact tcattaacgc ggcaagcgtt ctcgatcagt gaaatgacgt
     1321 ttagtatttc ctgaagcttc tgttgaatac cagtaagcga cactagcaac atcgtcttgc
     1381 cgctcaaata agcgaacatc atcgttgccg atttgttgta aagtgaccga taaatcttcg
     1441 gtaaaacaga tggggtcttg aagatgccaa cgataaagac catgtcgtgg cattgcgtcg
     1501 tcaccaaagg catgaacggc attcaatttt cccgtttcaa aacgatctct tgtggcatct
     1561 ctatttgatt ggtaaggata ccctaaaaag gctgttgaat agtttttcgc atgtggacgt
     1621 ccgaactcat cgtactcatg aaaggcccac gccccgccaa aataatcttc tgaacctgtt
     1681 gaggttacgg tcggatactc actatcccca tcaatgtaga atttaaactc gccttcgccc
     1741 caccaataac gttctaggca ggtaagcgca aggtaagtac ccacgtaata gccttgaccc
     1801 tttaatcgat caattaactt ataatcctcc tgtaatttgg taatgcgttc ccgatgccaa
     1861 tttgcgtgaa aatataggct attttcttcc aattcgtcca ccaaagcgta atttatcgta
     1921 taaaaaaatg aggacatatc cttcggatgt aaattctcga tcgtaatttt agcggatgtt
     1981 tggaaaggca tttcgaaata ggaattaaag ccccctgtag gattaaccac tatcggcaac
     2041 gagttcacct cgcaacgctc tccaaaccca ttacaaaaga aatcccctaa aggactttca
     2101 acagcagggc tatccgaccc atcccaataa attcttaaga ttaaatcacg taaaacaaaa
     2161 ctgccaaatt ctgttcgatc acgaatcgtc atccacatat gacgaattat tcccgttcca
     2221 gtaatatccg caatcacagt ttcttcacca gaagtcagcg gaattgcgcc acgacctttg
     2281 cggcttggtc ctagctcgct tggcaacatc gcagctttac ctttctcgcc tgttggattt
     2341 tcgggagtga ttgctcgact ctggattttt ttaaattggg taatattttt tagtatactc
     2401 atctttttct cctttgaatg tgaatcgttt ttcccttttc tacgtgaaga atttttttcg
     2461 tatcacttat tttcaatggc ttttctgaca aatttgaaat actcaactcg gattgcgtac
     2521 attcaatcga aatgaaggct tgaccgaact tcaaattctt ccaagaaaac gttaaattat
     2581 caacaggatc aatttgcaat actccttttt tgaatttcaa tccgaaatat tgactgatca
     2641 ttaggctgac aaaaagaccc gatgcccagc cgcatttgcc gcagccaaag tctcgcacga
     2701 cttctccttg ttgagcctct aagcgatacg gccaccacca ccacgaaccg tctaaatctg
     2761 ccaaacaaat tagttcttct aatcgggtta cgaaagcttg agggttgtgc agctcgctca
     2821 ttaaaacagt gataaagcca ggaaaagtgg cgccggattc taatccccaa gtgatgcctt
     2881 tgatttcctc gctataggtt ggattgaaag aactagcaga aaaggccatg gtgttctgat
     2941 ataaatgatc tttattatct aaaaattgat aaaaaggcat caacgtagtg tctgattctt
     3001 ctccatcatg catcaataaa ttaatttttc catccacaat cacatcactt aaaaagatta
     3061 acccttgctc aagaatcggt ttttgatagt gcttcactga ataggttgtt ttctcctcat
     3121 caccgattcc ctccaaaaat tgctcaccaa aaggaccatc gatagtcatt tgtttacaaa
     3181 ttgattcctt tagtttacag gcaatgtttt tgtatcgctt gctaagactc agttgttcta
     3241 gggcttcaaa aatttctgca agtccaatac aggccttcca aagacaaaga ttgctgccgg
     3301 tatgatactt tccaagtgca aaggcgtctg ataaccaaac agatggaaaa agcattggtt
     3361 cccctgaccg acgttgagct aatatgtcct caataattct tgaagcattc agtagaacct
     3421 gcggacaccg ctcaaagaat tttgtatccc cagtatgttc aaaataaaga ctagccagta
     3481 acgcactgga taacgaatta ctcagagaat gattgatacc tcctgtaaat tttgaaccag
     3541 gaaacttaac gccatattga tcaaaccata gaaccgtttt ttgacataac tccggttcaa
     3601 acatggtgaa tggcaatgat gcgtaataca tatccttatt ccaaattcga ttagtagcag
     3661 gatagctgcc ccaattgctg ccgacgacct gtccctgtcg attcataccg aaactagaaa
     3721 agctttgata aagtgcacga cgatagagat ggacaactct atcgtttggc attgacagct
     3781 gcccaaatag ctgagcaaaa tagttcaatg tttccttcag ccatttttcg atatcagatt
     3841 cctgaaaagt cattacttct tcaaaagcat ttgggtccac aaaagcaatg gcgaagcttg
     3901 ccacttcctg ttgtgcgatc gtgtccttat ttcctttttg agaaatcaac acattttgct
     3961 gatccgaata cttctcttga tacgaaggca catctttcac tgaaacttga aggctttcgt
     4021 gtgtttcatt cacaatgtaa acctgttgaa caagcatact taaacgctta ttagccagta
     4081 tgggagcaaa ggatactaca atgaccttaa acgtcgaata atgatgaata tatctaggca
     4141 aacagtcttg aatcacatcg acttcaatac gatgatccga ttctgacaaa tggtagacaa
     4201 tcccgtttac ttcaaccttt aaggataacg aattggtttg agagagctgt ttattgaccc
     4261 aaacacctgg tttttcctca gttagtaaat tttctcgata aaaggtcaac gtttgaatcg
     4321 tcccgcaaac atcaaggttt gctaatgtct tttgattact tacatcgaag gtcaatccct
     4381 ttacatcagg aatattccga tagtagctgt attcgtttga agtagtcaca tttccgtcgt
     4441 ttaactcagc attaaaaaat gggtgtttgg gtgaataaat tacttcatta atagtcatct
     4501 tttccctcct tgctaaaaaa actgcagcct agtagctgca gcttcctact atgcgattcc
     4561 aagtaatgac aataaaattc ccaagacgac cattccaaaa ataattttga tgacgccgac
     4621 ttttttatct agtaatttga aacagctcaa tgttaccaat aacggtacaa ttcctttaaa
     4681 aatactatcg aggatttctt ggacattcat tagttcttcg cccttcattg aaaagctcaa
     4741 aacggtattg aatttgacca tactgatcgt cattgcacct accatgatca gacccaagat
     4801 actagcagct tttgtcagaa tttcaattaa cccgttcgca tacagctttt caatatattt
     4861 agagcctaat gaatagccta aaacgccgcc atagtatcgc atcaagtaat taggtatatt
     4921 gaacataact aaaaagataa ttggtgccaa aatatttccg gcattggcta agttgatcgc
     4981 tagtcccgcg gtaacgacac gccaaactcc ccagaacaat gaatcgccaa ttccagccaa
     5041 tggtcccatc aaactcgttt tgatcgcgtt gatactttcc acgtcaaaac cagctttttc
     5101 agaattttcc ttctccatcg atgccgcaat ccccatgaca aaggacgcgc aaggaatggt
     5161 cgtcgaaaaa tactgtgtat gacgttccag cgccgcagct cgatcttctt cacttttgta
     5221 gaaatgatta atgaagggca tcatagaata ggcgaaacca ccgccgccca tcgtcaccgg
     5281 attcaccgaa gcatacatag tcatcgagcg ccaaaacatc ttccgaacca attttttctc
     5341 atctttagaa attccatcag ctaatttcat tgaaagaaat cctcctcttc ttggtcaagc
     5401 gttgcattta cagcagcatc gctttgaact gcatgaacag atatattttt tagttgtaga
     5461 tctctttggg ctataacaat tgcaataatc gttccaactg cggcaattgc aatcaatggt
     5521 aactcgagat acgccataac aacggcccca aagaaaaacc acacagcaat atttttactc
     5581 caaagcatcc gtaacagcat cgccatccca actgccggca gcagatttcc agcgactgtt
     5641 aatccgttca tgatgacctc tggaatatta tccaagacgc ttttgacaac atctgctcca
     5701 aataaaacgg caaagaatgg aataagagca taaagcgcat agtggacaaa ccacataaag
     5761 aagtgcagat tgatgattcc tttttgatca ccagattttg caaatttgaa aaatttagca
     5821 gccatcggtc cacatacaaa agaaaaaatc atattcatta actgtaatcc taatactgct
     5881 agtggaaaga caatgggaat aatcgcatcc acgtttcctc ccattttgat tgcgaacgct
     5941 gtagccagcc cagcagccaa agaaggctcc gccgtactag agattccgat attgacgaca
     6001 cccataaaga tggcttctag tgacgccccg acaatcaacc ccgtctgcaa atcccccatc
     6061 aacgctcctg ctaacggagc aacaacaatc ggacgactca gcatggacat cccgaacatt
     6121 tcatgtccag caactccaat aaaaacggcc aaagcgacta caaatgcatc atacataatt
     6181 tttctccttc ctttttctaa aaatcactca aaatagagat agctgaacgt ttttccatcg
     6241 ccggtgtagg ctgaaaaacg gtatctggat aaagttcaac taattctttt aagtcttgaa
     6301 tttctgacgt cgttaacatc acttcctttg ataaggtttt cttatcttcc gatggcggtg
     6361 tcattttccc agcattcccc acgttcatta attcgatttc cgttgggaaa gctttcgcaa
     6421 cttttaccgc atctttgaca ttagggacta aaaccatcaa gcgcatgtcc ttcgcccgcg
     6481 gatcttttaa caaatgaatc gcgtcatcaa ctgttttaat aatgactttg acattcgatg
     6541 gtgcagccat cttcaatgtc atcttttgaa tatcgttggt agccgcctca tcgcttgcta
     6601 ctactaaccc ggttaaattt agactctttg tccaagtcat cgctacttgt ccatgaatta
     6661 atcgttcatc aattcgcact gctttaatca tttttcttcc tcctaaaaaa aatcttcctc
     6721 ttcatttact acatgtacct catcaagaat ggtcatcttt aattcctgtt ggcatttatt
     6781 taccagttcc tgaatatcct cctttgttaa ttccttggtt gccaatacta tttcgagcag
     6841 aattggcaga ttaaagccag acaaaatagt tacagcaatc tctttctcca aaaaggtttt
     6901 ggtaatcttc tgattgacac ttccgccaaa taaatcggta aaaacaaaaa tctgatcctg
     6961 ctcgttcacc tggtttacaa atacattcaa ctcctgttca aaatcacact catccacata
     7021 agcattaatc accgtaatct cttgaacctt ccccgtcagt aattgaatac tgctaagaaa
     7081 gcctgaagct aagtgaccgt gagtcgctat tagaattttc ttcatatggt taattccttt
     7141 ctttctgttc acttttatat aagcaattcc tgtgccaact tttaaaaaca ttggtttaat
     7201 cacgttaatt cacattaaaa aaagtgtcta acctaaaaaa aacagattag acactttttt
     7261 agac
//
LOCUS       DAYYFD010000120         6892 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000120 DAYYFD010000000
VERSION     DAYYFD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.123"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..1216
                     /locus_tag="ACHA25_002624"
     CDS             62..1216
                     /locus_tag="ACHA25_002624"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8763871.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1238..1990)
                     /locus_tag="ACHA25_002625"
     CDS             complement(1238..1990)
                     /locus_tag="ACHA25_002625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8763872.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2354..5020)
                     /locus_tag="ACHA25_002626"
     CDS             complement(2354..5020)
                     /locus_tag="ACHA25_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8763873.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5361..6812
                     /gene="cls"
                     /locus_tag="ACHA25_002627"
     CDS             5361..6812
                     /gene="cls"
                     /locus_tag="ACHA25_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8763874.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2036 a         1416 c         1251 g         2189 t
ORIGIN      
        1 ttaatctttt tatgatacct tagtaaagta agttttttat cataagtaaa gaggtgttga
       61 aatgaaaata gggattgatc gtctttcctt ttttattcct aatttatatt tagatatgac
      121 agagttggca gaaagccgtg gggatgatcc tgcaaaatac catataggga ttggtcaaga
      181 ccaaatggca gtcaatcgtg caaatgaaga catcattaca ctaggagcaa atgctgccag
      241 caaaatcgta acagaaaaag accgtgagct aatcgacatg gtcatcgtcg gaacagaatc
      301 cgggattgat cattcaaaag cgagtgcggt aattatccac catttactga aaatccaatc
      361 ttttgctcgt tcttttgaag taaaagaggc ttgctacggt ggcaccgcag ctttgcacat
      421 ggcgaaagaa tatgtcaaaa atcatccaga acgaaaagta ctagtcatag caagtgatat
      481 tgctcgttac agcttggcaa gcggtggtga agtgacgcaa ggtgtcggtg ctgttgcgat
      541 gatgatcact caaaacccgc gtattttatc gattgaagac gacagcgtat ttctgacaga
      601 agatatctat gatttctggc gtccagatta tagcgaattt cctgttgttg atggtccttt
      661 atctaattct acgtatatcg aatcattcca aaaagtttgg aatcgacata aagaattgtc
      721 gggtcgagga ctcgaagatt atcaggcgat tgctttccat attccgtata ctaagatggg
      781 aaaaaaggca ttgcaaagcg tattagacca aacagacgaa gaaaatcagg aacgtcttat
      841 ggctcgctat gaagaaagca tccgttacag ccgacgaatc ggtaatcttt acactggttc
      901 attatacctg gggctaactt ctctactgga aaattcgaaa tcactacagc caggagatcg
      961 catcggtcta ttcagctacg gttctggtgc agtaagtgaa ttttttacag gttatctgga
     1021 agaaaactat caagaatatc tcttcgcaca atctcatcaa gagatgctag attctcgaac
     1081 acggatcact gttgacgaat atgaaacgat tttcagtgag acgcttcctg aacacggaga
     1141 atgtgcagaa tatacttcag atgtaccttt ttcgattaca aagatcgaaa atgatattcg
     1201 ttactacaaa atataataaa atcataaaaa gaacccttca cgagtcgtga aaggttcttt
     1261 ttttatctgc ggaccaaagg agcagtctat aggtcagctt agtcgtacta aggatttctc
     1321 gtttttcact gaatagtttg cgaatgtacc tggtcacaac aggatctttt gcgtacgcgt
     1381 catagtatag cttcatcaat tcttcagtaa ttgattcctg tgtaaaataa gtaaaatctt
     1441 ttttcttcac tttgaatcct tcatcaatta agtatgtttc aaattgatcc attacaactg
     1501 gcgatgtatt aggatcttct tctatcattc ctagttgctt ttcgatagga taaaaaacat
     1561 catcgattga ttccactact ctgattatca cgcaccattt gcttgacttt ttcatcaatt
     1621 tttctaattg aataagatgt accaatgctg gatttgctgc tgtaaaaacc atttcatatg
     1681 tctcatttga ttggataaat tcttctaacg actgtttgat aaaggaaaca tttggcaact
     1741 catttttttc tgcttctttt tttgcatttt ctagcattcc tgaagaaaaa tcgatcaaat
     1801 catagcgatt aatttctttt gctaaggaaa gaaatctgcc agatcctcca gcaacgtctg
     1861 ctgctgtttc agcaggcagg actttctccg ttttcagata attttttaca tcttcagcaa
     1921 tagttacttg cgattcttgc tggatttcat aatattcttc agcaaattca tcccattctt
     1981 tttcattcat ttgatttcct ctaatttttt gttatatgtt ttcacttttc ggaataaatc
     2041 attctcctct tttatctatt ctatctcttt tgatgaataa taaaatgatt ggctttccgt
     2101 ctatcataaa taaaaaaagg gactgtgacc aaagtcgtgt caaagtccct ctttccgaat
     2161 aaacagtgtc caatcagatg accctcagta ttagctcaaa atgctgattt tacaacttct
     2221 tccttaaacg gtgttctcct gccaactctt cggtcacaag tcacaatatt caacaaacat
     2281 gagaagctgt ttgttgaaat tgcaccttgt gactcagaat taaacggcag gctcacaacc
     2341 tcgttgattc gttttattta tttccttgta ctcgtacaat gatcaattta gtgatttcca
     2401 tcaaaacaat tgtcacaagt gcgattccca tacatgtaaa gacttctgtc aatccaaatg
     2461 ctgatgggat agagaagact tctttcagtc ctgggatcat tgttaagcag taaagcgcgc
     2521 tacatacaac tacagctagt aagacacttc tgttagagaa gaaccctgct tggatagctg
     2581 tttgtgtgtt tgaacgtgct gggaatgttt gcaatgtccg acaccaaatc aatgttgaaa
     2641 atgacatggc tacaccgaat tcaggtgaat gctgcatgcc aagccattga gcaaagataa
     2701 cagcaatccc aatcagcaca ccacgatacg ttacagaaat caatgttttg tccgtaaaga
     2761 ttcctgaatt tggatctctt ggcgggcggt tcatcgtagc aggttcagct ttttctgtac
     2821 ctaatgcaat tgctggcagt gaatcattga ctaaattgat gaacaacagt tgtaatgctg
     2881 taaatggatt ggaccagtca gcaactaagg caaagatgat cgcaatgatc gcacctaaat
     2941 ttcctgcaaa tagataagaa atagctttct tgatattatc aaacacatta cgtcctactt
     3001 ctactgcgct gacaatagaa gcaaagttat catccgtcaa taccatcgct gctgcatctt
     3061 ttgccacatc tgtacccgtt cccatagcaa ttccgatgtc tgcttgtttc aatgctggtg
     3121 cgtcatttac accatcacct gtcatagctg agatatgacc tttattttgc caagcacgaa
     3181 cgatacgaat tttattctca ggtgaaacac gtgcataaac agtgactttt tctaaaactt
     3241 cgtttagttg atcttctgtc aattcatcta gttccgtccc ggtcaaagcc aggtttcctt
     3301 cttcaaaaat cccgatttgt tttgcgatag caacagctgt tgttttgtgg tcaccagtaa
     3361 tcatgattgt tttgattcct gcacttcttg cttctgttac cgcctgagtc acttcagcac
     3421 gtggaggatc gatcatagca agcaaaccaa caaggatcaa atcgtcttca tcttcaaatg
     3481 ataaatcaaa attatcaagt ggacggtaag caaaagctag tacacgtaat gcgcgtgttg
     3541 aaaaagcttc attttgctct tggaattttt tcatcaattc atccgtcatc ggaacgactt
     3601 caccatttag caatactttt ttactgcgtt ggaagacgat atctggtccg cctttaacaa
     3661 acaagtaatt tttgttatca attgtgtgtc cagtagacat taattttcga tctgagtcaa
     3721 aaggaagttc tgcttgtctt gggtagttgt ttcggatttc ctgataaggc tggtttaatt
     3781 tttcactaaa gtcgataaac gctacttccg tagggtcgcc caatttagta ccgtcttcac
     3841 tgatcgatgc atcattcgct aaaacactga tttcgatcaa gcgacgttcg tcagctgtcc
     3901 attttgaagg gtcatttgtg aaatccccag tatcgccatt acctaaaaag tagtcaacaa
     3961 ctgtcatttt gttttgtgtc aatgtaccag tcttatctgt acagatgata cttgtcgaac
     4021 caagtgtttc aactgctgga agcttccgaa taatggcatg acgttttgcc attttattcg
     4081 ttcccaaaga taacacgatt gttacgatag actgtaatgc ttctggaata gcagctacgg
     4141 ctacggctac ggcaaacatc actgcgctga cgatatctgc agtcatatct cctgtacctt
     4201 ctccaagata aatacgaaac agttggatac ctaaaatcaa taaagacaat gccaaaatag
     4261 ccaaacttag ttttttactg aatttatcca gacctcgttg taacggagtg acttgctcag
     4321 ttgttgattc caaaaggcca gcaacttgtc cgatctccgt attattccca gttgaagtca
     4381 ctaagaattt accgcgtccg taagttacga tcgttccact gaataccata tttgtacgat
     4441 ctccgattgg taccgtactt gtgatatccg ttacttcttt ttctgcagaa gtcgactctc
     4501 ctgtcagcat accttcatct acttttaatg atcccgcatc tagtaaacgt ccatcagcag
     4561 gtacataatc gcctgcgtct aacaacacga tatctcctac cacaagttcg tttgccggaa
     4621 tcgttttttc cttaccatca cgaatcactc gagcatcggg cgctgaaagg tttttcaacg
     4681 cttctaaaga accttctgct ttttttgttt ggacaacact gattatagaa ttcaacatca
     4741 atactgcaaa aataactaag aattcgatgt agtcacccat gaccatctga atcagtgcga
     4801 caatcagcaa tacgataacc attgcatctt tgaaagtttc taagaacaat ttccaagttg
     4861 gcttcttcgg cttttctttt agctggttca agccatctct ttctaatttt tccttcgctt
     4921 gcgtggtgct caatccatcg gttgagctct gcgtttgctg catcaacgag tcgatcgttt
     4981 ctttatagac cgcttgttgt gcttgttcct tgttttccaa atttcatccc ccattttttg
     5041 atcttggccc tagtcctgtc tgaaaaaaaa gagacctaag acaaatacgc atttgtctta
     5101 ggtctcacca tttcatacaa taccagaaaa ccatttggtt aacttactgt tgatattatc
     5161 gcaacaaacg ttgccggtta ctcccctaag gagatattca actgtactaa tatcctacct
     5221 tattaaccta caaaaagcaa ctactttagc ccgatttacc tacattttca tgaaaggagt
     5281 gaaaagtata ttcaccttgg taggcttctt tcttctatca tgctatacta atgttattgt
     5341 atgtaaggag gaacattttt gtggtatcta gtattataac cgccctttat ctattaaatg
     5401 cacttattgc tttggtggct attttgatca aaccccgaga tgtagcagcc atttgggcat
     5461 ggctcttagt attgtttgcc cttcctgggg tgggctttgt attatattta tttttcggac
     5521 gtggattaac ggataagaaa aaattctatc tccgacaaag tgacttgaaa gaattagaaa
     5581 actttcagtc ttttagagga gataccattg aacattacga tcctgacatg ggcgataaag
     5641 acaaacagca atttgttgac ttcttctctt cattaaatcg tatgccgctg acaagaatga
     5701 attctgtcac tcttctcaca gacggacaag agaaattgga ttcactgctt caagatctaa
     5761 aaaaagccaa acattcgatc catatcgaat attacgcatt tgtgacagat aatatcggcc
     5821 agcaagtctt acatgtttta gaagaaaaag ccgcagaagg cgtggaagtt cgaatattat
     5881 atgatgcatt tggctctcat ggcacaaaag caaaagattt caatcgtcta atcaaaaatg
     5941 gtggacatgt ccatacattt gttacctcac aaagggcatt acttcgtttc cgattgaatt
     6001 accatgatca ccgaaaaatc gttgtgatcg atggaaagat tagttatacc ggtggtttca
     6061 atattgccaa tcaatatgta aatacaacaa aaaaattcgg ctattggcgc gatacgcata
     6121 tacggatttt cggtgccgct tcttctttgc tccagcttcg cttcttaaca gactggaacg
     6181 tctcggtacc tgaagaaaaa aaggtcggct atcatttgaa ttatttcttt aaaaaagcag
     6241 atcgagatga atctaagctt gctgatacat ccatccagct tgtttcaagc ggaccgaata
     6301 acgaaaggga acaaatcaag ctttcattta tcaaattgat tacttctgct aaaaaacgtg
     6361 tttggataca gacaccttac cttgttcctg atgaaagtgt cattgctgct ttaaaaatcg
     6421 caactgcctc tggtgtagat gtgaaaatca tgattcccaa caaaccggat catcctttta
     6481 tttatcgagc aacacaatat tatgctcggc agctgatcaa ggaaaatgta caaatccttg
     6541 tctatgagaa cggcttcctc catgcaaaaa cattgataat ggatgatgaa atctgcatgg
     6601 taggttcagc aaatcaagat attcgtagct accgattgaa ttttgaaaca agtgctgtca
     6661 tttacgatcc tgagttttta gaagaacttg ctactcagtt caaagaagat gagacacatt
     6721 gttcatccat gacaactgaa acagtcaagg aaatgtctaa ctggctatta ttcaagcaac
     6781 aaatttctcg attattttct ccaatcctat aattttttta ttcaagtaga gaaaaatata
     6841 ctaagattaa agggactgtg acaaaaatct tgtcacagtc ccttcttccg aa
//
LOCUS       DAYYFD010000121         6684 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000121 DAYYFD010000000
VERSION     DAYYFD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.124"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACHA25_002628"
     CDS             <1..615
                     /locus_tag="ACHA25_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8763875.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACHA25_002629"
     CDS             612..1385
                     /locus_tag="ACHA25_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8763876.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACHA25_002630"
     CDS             1382..1780
                     /locus_tag="ACHA25_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8763877.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACHA25_002631"
     CDS             1965..2636
                     /locus_tag="ACHA25_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763878.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACHA25_002632"
     CDS             2695..3135
                     /locus_tag="ACHA25_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763879.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACHA25_002633"
     CDS             3147..3815
                     /locus_tag="ACHA25_002633"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8763880.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACHA25_002634"
     CDS             3879..4502
                     /locus_tag="ACHA25_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8763881.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACHA25_002635"
     CDS             4558..4944
                     /locus_tag="ACHA25_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8763882.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACHA25_002636"
     CDS             5157..6548
                     /locus_tag="ACHA25_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8763883.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2128 a         1081 c         1488 g         1987 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttg
//
LOCUS       DAYYFD010000122         6591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000122 DAYYFD010000000
VERSION     DAYYFD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.125"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHA25_002637"
     CDS             complement(1273..1938)
                     /locus_tag="ACHA25_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763884.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACHA25_002638"
     CDS             2064..4100
                     /locus_tag="ACHA25_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8763885.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHA25_002639"
     CDS             4104..4742
                     /locus_tag="ACHA25_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763886.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHA25_002640"
     CDS             4770..5198
                     /locus_tag="ACHA25_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763887.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHA25_002641"
     CDS             5655..6566
                     /locus_tag="ACHA25_002641"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8763888.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2265 a          999 c         1219 g         2108 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac t
//
LOCUS       DAYYFD010000123         6437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000123 DAYYFD010000000
VERSION     DAYYFD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.126"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA25_002642"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACHA25_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHA25_002643"
     CDS             complement(1878..3080)
                     /locus_tag="ACHA25_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8763889.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHA25_002644"
     CDS             complement(3140..3343)
                     /locus_tag="ACHA25_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8763890.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHA25_002645"
     CDS             complement(3485..4552)
                     /locus_tag="ACHA25_002645"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8763891.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHA25_002646"
     CDS             complement(4610..4717)
                     /locus_tag="ACHA25_002646"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763892.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHA25_002647"
     CDS             4880..5692
                     /locus_tag="ACHA25_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763893.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHA25_002648"
     CDS             complement(6066..6257)
                     /locus_tag="ACHA25_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763894.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1931 a         1314 c         1009 g         2183 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaa
//
LOCUS       DAYYFD010000124         6417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000124 DAYYFD010000000
VERSION     DAYYFD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.127"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1126
                     /locus_tag="ACHA25_002649"
     CDS             <1..1126
                     /locus_tag="ACHA25_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8763895.1"
                     /translation="FLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFT
                     INFNHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRI
                     MIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISN
                     IGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLA
                     KFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILL
                     NMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSL
                     ILHILINQMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            1139..2146
                     /locus_tag="ACHA25_002650"
     CDS             1139..2146
                     /locus_tag="ACHA25_002650"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8763896.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2158..3162
                     /gene="neuB"
                     /locus_tag="ACHA25_002651"
     CDS             2158..3162
                     /gene="neuB"
                     /locus_tag="ACHA25_002651"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8763897.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGLQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            3159..4307
                     /gene="neuC"
                     /locus_tag="ACHA25_002652"
     CDS             3159..4307
                     /gene="neuC"
                     /locus_tag="ACHA25_002652"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8763898.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            4280..4957
                     /locus_tag="ACHA25_002653"
     CDS             4280..4957
                     /locus_tag="ACHA25_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8763899.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            4908..6089
                     /locus_tag="ACHA25_002654"
     CDS             4908..6089
                     /locus_tag="ACHA25_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8763900.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            6102..>6417
                     /locus_tag="ACHA25_002655"
     CDS             6102..>6417
                     /locus_tag="ACHA25_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8763901.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEV"
BASE COUNT         2325 a          814 c         1212 g         2066 t
ORIGIN      
        1 gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg atatgaacca
       61 acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta gttatatcca
      121 gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt ttatatctac
      181 ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg atgataaata
      241 tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag taatttattt
      301 aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat acgctttaaa
      361 aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac aattcgatag
      421 aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct acattagtaa
      481 cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg ttccatggtt
      541 ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta actattattt
      601 agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt tagcaaaatt
      661 tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa ccttaggcta
      721 tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga aaaaaacatc
      781 gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa acatgatttt
      841 catacctagg ttaggaataa ttgctgctgg atacacaacc gtatttacgt attttttact
      901 ttttattttt cattataata tcgctaaaaa aattacgaat aaacaattat ttgacacaaa
      961 aaaaatatgt atcataataa tttttgtatc aattttttct ttgatacttc atattttaat
     1021 caatcaaatg ttgattcgtt acgttttgac atcattattg ataattagtt tagttatata
     1081 ttttttaaag attaagaatc gaattataga tgactataat tcataggagg agatataagt
     1141 gtttaaagga aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt
     1201 tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa
     1261 acaattttat atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga
     1321 tatcagagac tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc
     1381 tgccgcaatg aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa
     1441 tgtgatggga actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt
     1501 aggtatcagt acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt
     1561 gagtgaaaaa ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc
     1621 ttgtgtaagg tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa
     1681 acaaataaaa aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat
     1741 gtcaataccg gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga
     1801 aacatatgtc ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa
     1861 aatatataat gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag
     1921 gccaggagaa aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga
     1981 aaatgataaa atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga
     2041 ttatgaaaaa attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca
     2101 ggaatttatt agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg
     2161 aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta
     2221 gctttagaaa gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca
     2281 tttaaaacga ataagctagt ttccaaaaag gctccaatgg caaaatatca gaaggataat
     2341 cttaaagagg acagttctca atttgaaatg ctaaaaaaat tagaattatc tcaatctgat
     2401 tttattagaa taaaagatta ttgtatacaa aataaaattg attttttgtc tactcctttt
     2461 gatgaagaaa gtgcagactt tctaaaacaa attggtctac aaggatttaa aattggctca
     2521 ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta
     2581 ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat
     2641 agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat
     2701 ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat
     2761 tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa
     2821 cattttacct tggataagga actacctggt ccagatcata aagcttcttt agatcctcag
     2881 gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt
     2941 aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc
     3001 actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct
     3061 ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca
     3121 ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa
     3181 gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata
     3241 attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta
     3301 aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag
     3361 agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata
     3421 tatttgaaca aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg
     3481 cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag
     3541 tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt
     3601 ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag
     3661 tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta cctagtttta
     3721 gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt ttgcaagtat
     3781 ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct ttgatcaatt
     3841 caatggaaca acaatctcaa ccaattattt gcattttacc taatagtgat gctggtagcg
     3901 aaattatcag aaaaatgtac cagaaatatt taaaaacgga tattatttat attacgaatt
     3961 tagagccgga ggaatacctg actatattaa aagagtgcaa atttatgatc ggaaattctt
     4021 cttctgggat aattgaagct gcatcattca aaataccagt aataaattta ggaaccagac
     4081 aaaatggacg agaacaatct aaaaatatta taaatgcaaa tctaaatgaa aatgaaattg
     4141 aaactgcttt gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga
     4201 acatctacgg tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga
     4261 atgatgggga agatattaag tggattcaaa aacaaatagc ttattaaaat atactagtga
     4321 tccatacatt attattgggg ctggcggaca cgcaaaagtc attattgaaa ccttaagatt
     4381 agaaaataag ttaatatatg gattttttga tgataagcaa aataaaatgc aagatctgaa
     4441 atatcttggg aaaataaacg aattgaatag ctgggcggat agcaaaaaaa aaatctataa
     4501 atttattgta gctattggtg actgtgtagt aagaaaaaag attataaaaa aattgaatat
     4561 gccaagtgag caatatggaa tagctgttca tccaagtgct atagtggcaa aagaaatttc
     4621 aattggttat ggaactgtta tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg
     4681 agagcatgct ataataaaca caggtagcat tgttgagcat gattgtagaa ttaataacta
     4741 tgttcattta tctccaggtg tatgcttagc gggtggagta catgtaggtg taggaactca
     4801 aataggaatt ggttctcaat gtatccagat gaagaaaatt ggatcttggt gtatgatagg
     4861 cgcagggagc actattgtta aggatattcc ctcacattcc ttagcttatg gcaatccagc
     4921 taaaattaag aaagaaggaa ttaattttga attctagaat tttattatca agtcctcata
     4981 tgggaggaac agaagaaaaa tatgttgcta aggcttttaa agataattgg atagcgccat
     5041 taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa atatactggt tctaagggcg
     5101 gagcttctgc gttaagttca ggtacagctg ccatacatct tgccttaaga ctgcttaatg
     5161 ttggaaaaaa cgatattgtc tttgctcctt catttacttt tatagctaca gtaaatccta
     5221 ttttatatca aggggctgta ccagtgttca ttgactcaga acctaataca tggaatatga
     5281 gccctgttgc actagagagc gcattagaag aagctgaaag aaacaaccaa ttacctaagg
     5341 caatcatcgt agtgaattta tatggtcaaa tggctgatta tgaacgaatt ttagctattg
     5401 caaaccaatt taatgttcca gtgatagaag atgcagcgga atcgttaggt tctacttata
     5461 agggaatttc gtcaggctta tttgggacaa ttgggatcta ttcatttaac ggtaataaga
     5521 tcatcacaac ctcaggtgga ggagctctag tgtctagtga taaaaaatat gttgagcatt
     5581 caagattttt agcaacacaa gcaagagatc aagcaccgta ttatcaacat agcgaaatag
     5641 gttataatta ccgtctaagc aatatttctg ccggtatcgg acgtggacaa atggaagtat
     5701 tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg atataaggaa gctttttctg
     5761 gaattgatag aattgaaatg atgccagaag tggatggcat ggtgccaaat cgttggttgt
     5821 ctactttaac gatcaatgat tataattcta ttgatcaagt agttaacgta ataaataaac
     5881 taaatgaaaa aaacattgaa acaagagctt tgtggaaacc actacatttg caaccagtat
     5941 ttcaaggggt aaaaatgtat taccatgata ataatcttag tgtgtcagaa aagttattta
     6001 atagcggctt atgcttacct tctggaagta atatgacaga tagtgaacaa gaatttgtta
     6061 ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt tatgaacata ggaattattg
     6121 gtttaggttc catggggaaa agaagactta gactcattca agaacacttt aatgatattt
     6181 tactatgtgg tgttgatact aatgaggcga gacgaaagga agttgaaaag gaatttggaa
     6241 taccaactta taatagtcta acagaggcaa aaaatttgtt tgaaataaat tcagtttttg
     6301 tttgtacatc ccctataacc catgaattta ttataaaaga ggctttaaat ttagattgta
     6361 atatctttac ggaaatcaat ttattaaatg aatattatga tgaaataatc gaagtag
//
LOCUS       DAYYFD010000125         6387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000125 DAYYFD010000000
VERSION     DAYYFD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.128"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACHA25_002656"
     CDS             330..1175
                     /locus_tag="ACHA25_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8763902.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACHA25_002657"
     CDS             1456..2676
                     /locus_tag="ACHA25_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8763903.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACHA25_002658"
     CDS             2673..5393
                     /locus_tag="ACHA25_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8763904.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACHA25_002659"
     CDS             5377..6321
                     /locus_tag="ACHA25_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8763905.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2378 a          987 c         1232 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccagcaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DAYYFD010000126         6334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000126 DAYYFD010000000
VERSION     DAYYFD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.129"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACHA25_002660"
     CDS             complement(29..1261)
                     /locus_tag="ACHA25_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8763906.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACHA25_002661"
     CDS             1516..2085
                     /locus_tag="ACHA25_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8763907.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACHA25_002662"
     CDS             complement(2263..2703)
                     /locus_tag="ACHA25_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8763908.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACHA25_002663"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACHA25_002663"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8763909.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACHA25_002664"
     CDS             3657..4580
                     /locus_tag="ACHA25_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8763910.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACHA25_002665"
     CDS             complement(4805..5044)
                     /locus_tag="ACHA25_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763911.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACHA25_002666"
     CDS             complement(5049..5468)
                     /locus_tag="ACHA25_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8763912.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..>6334)
                     /gene="mef(H)"
                     /locus_tag="ACHA25_002667"
                     /gene_synonym="mefH"
     CDS             complement(5695..>6334)
                     /gene="mef(H)"
                     /locus_tag="ACHA25_002667"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8763913.1"
                     /translation="VIAVAKNDLKDSYTFLKKEKPIFISVIFLVCIFNLVLSAVLIVG
                     LPILISEVLKMPDTLYGFSQSALSLGGLCGGILTALVASKLKLKNLYTLLLICSVSVL
                     LMGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQTPPQLVGKIMATLV
                     AVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKRIFQKLEI"
BASE COUNT         2000 a         1190 c         1138 g         2006 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat taca
//
LOCUS       DAYYFD010000127         6066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000127 DAYYFD010000000
VERSION     DAYYFD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.130"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACHA25_002668"
     CDS             complement(254..541)
                     /locus_tag="ACHA25_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8763914.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACHA25_002669"
     CDS             complement(561..785)
                     /locus_tag="ACHA25_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8763915.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACHA25_002670"
     CDS             complement(1240..1410)
                     /locus_tag="ACHA25_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763916.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACHA25_002671"
     CDS             complement(1425..1958)
                     /locus_tag="ACHA25_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8763917.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACHA25_002672"
     CDS             complement(1979..2923)
                     /locus_tag="ACHA25_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8763918.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACHA25_002673"
     CDS             3560..3646
                     /locus_tag="ACHA25_002673"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8763919.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACHA25_002674"
     CDS             complement(3767..4456)
                     /locus_tag="ACHA25_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763920.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4701..5615)
                     /locus_tag="ACHA25_002675"
     CDS             complement(4701..5615)
                     /locus_tag="ACHA25_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8763921.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACHA25_002676"
     CDS             complement(5597..5977)
                     /locus_tag="ACHA25_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8763922.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DAYYFD010000128         5890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000128 DAYYFD010000000
VERSION     DAYYFD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.131"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..574)
                     /locus_tag="ACHA25_002677"
     CDS             complement(116..574)
                     /locus_tag="ACHA25_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8763923.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(587..1918)
                     /locus_tag="ACHA25_002678"
     CDS             complement(587..1918)
                     /locus_tag="ACHA25_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8763924.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1929..3371)
                     /locus_tag="ACHA25_002679"
     CDS             complement(1929..3371)
                     /locus_tag="ACHA25_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8763925.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3358..4122)
                     /locus_tag="ACHA25_002680"
     CDS             complement(3358..4122)
                     /locus_tag="ACHA25_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8763926.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4226..5101)
                     /locus_tag="ACHA25_002681"
     CDS             complement(4226..5101)
                     /locus_tag="ACHA25_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8763927.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5356..5440)
                     /locus_tag="ACHA25_002682"
     tRNA            complement(5356..5440)
                     /locus_tag="ACHA25_002682"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5404..5406),aa:Leu,seq:caa)
     gene            complement(5458..5529)
                     /locus_tag="ACHA25_002683"
     tRNA            complement(5458..5529)
                     /locus_tag="ACHA25_002683"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5495..5497),aa:Gln,seq:ttg)
     gene            complement(5539..5611)
                     /locus_tag="ACHA25_002684"
     tRNA            complement(5539..5611)
                     /locus_tag="ACHA25_002684"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5576..5578),aa:His,seq:gtg)
     gene            complement(5673..5757)
                     /locus_tag="ACHA25_002685"
     tRNA            complement(5673..5757)
                     /locus_tag="ACHA25_002685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5721..5723),aa:Leu,seq:caa)
     gene            complement(5778..5848)
                     /locus_tag="ACHA25_002686"
     tRNA            complement(5778..5848)
                     /locus_tag="ACHA25_002686"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5814..5816),aa:Cys,seq:gca)
BASE COUNT         1799 a         1254 c          897 g         1940 t
ORIGIN      
        1 tcgataatta acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa
       61 aaattttgta aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa
      121 attcaaaaca actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa
      181 aaatgcctgg agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca
      241 aatctataat agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct
      301 gaaacggact tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc
      361 cagattcact ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt
      421 taactggcgc atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt
      481 ttgaagaaaa gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag
      541 cttttagaat gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg
      601 gccaatattt tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat
      661 ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt
      721 aagcatctac tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat
      781 cactgattgg ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta
      841 tttcaacaac tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca
      901 taaagcgacg acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat
      961 gctccccaaa attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca
     1021 tttctttggt atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt
     1081 cagtatgctc aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat
     1141 tacttggaaa atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat
     1201 ctcctatatt aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg
     1261 gcataatttc tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta
     1321 aaccataata cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt
     1381 cttcaattga aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac
     1441 gaacagcttc agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg
     1501 catagtcttg tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag
     1561 caaatcctcc gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt
     1621 cgtccttttc tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc
     1681 ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc
     1741 ttttcagcat aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat
     1801 ataaacgtat ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc
     1861 ctggtgtata cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct
     1921 tttcctttct attcttctaa taatttattg aattctgcca taacataagt cacttctaag
     1981 ccgatgataa tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc
     2041 ttgattcgct gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta
     2101 caactataga catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc
     2161 atatattcat aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg
     2221 ccaaactctg ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa
     2281 taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca
     2341 ttggtagcga ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca
     2401 attcgtatat tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc
     2461 acgtacaaca taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca
     2521 ataaatgaag ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta
     2581 taagctgctt tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa
     2641 aaaactgata aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct
     2701 ctgaaaactt ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc
     2761 aacacatggt gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca
     2821 cctaatgctc ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc
     2881 ggccagataa agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat
     2941 gggaattttt cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga
     3001 ttatacgtcc atgccgctac taaaccggaa ataattccac caaaaatccc catattcact
     3061 tctgggtcca taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt
     3121 gcaggtattc ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag
     3181 atatccaagt ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata
     3241 tcgtcagctg ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc
     3301 atcgctcttc caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta
     3361 aatatcctca tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc
     3421 tgaatgaatt aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa
     3481 caattcctga tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa
     3541 tatcatgagt aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc
     3601 atcagtttct cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc
     3661 ttttcgttca aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac
     3721 ctcctgattt tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat
     3781 ccccgcatga taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc
     3841 aatgttaaat ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt
     3901 atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag
     3961 cctaggcatt tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa
     4021 tgtagttgat gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg
     4081 gctatagcca aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt
     4141 tctactaaaa taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc
     4201 gcctatagat agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga
     4261 tgatttcatt ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta
     4321 gtgctgtaca atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta
     4381 ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc
     4441 gatgataagt cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca
     4501 aaatcgtggt cgtcatttct actattttta agctctttaa actattaggc tctcctagct
     4561 cagcattaat gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat
     4621 atcgatgatt gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga
     4681 atctcatttc tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag
     4741 caaaataaat atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc
     4801 tagctgttat cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg
     4861 agactatctc actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag
     4921 catcctcaac atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg
     4981 caattttttc tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca
     5041 catacaccgt taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca
     5101 tacatatttt tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt
     5161 tcttaggtcg tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca
     5221 cataataaaa gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat
     5281 tgatttggag gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg
     5341 ctatattgat aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg
     5401 attttgagtc cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg
     5461 gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc
     5521 tataccccaa gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa
     5581 tccggtgcgt taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca
     5641 ataaaaaact aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg
     5701 ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag
     5761 ttgatttatt agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac
     5821 ctctgcctta ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa
     5881 taaattatag
//
LOCUS       DAYYFD010000129         5781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000129 DAYYFD010000000
VERSION     DAYYFD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.133"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..539
                     /locus_tag="ACHA25_002687"
     CDS             51..539
                     /locus_tag="ACHA25_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8763928.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            835..1116
                     /locus_tag="ACHA25_002688"
     CDS             835..1116
                     /locus_tag="ACHA25_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763929.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            1118..1534
                     /locus_tag="ACHA25_002689"
     CDS             1118..1534
                     /locus_tag="ACHA25_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763930.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            2148..3302
                     /locus_tag="ACHA25_002690"
     CDS             2148..3302
                     /locus_tag="ACHA25_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8763931.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            3275..3718
                     /locus_tag="ACHA25_002691"
     CDS             3275..3718
                     /locus_tag="ACHA25_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763932.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4852..>5781
                     /locus_tag="ACHA25_002692"
     CDS             4852..>5781
                     /locus_tag="ACHA25_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8763933.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKE"
BASE COUNT         2157 a          983 c          943 g         1698 t
ORIGIN      
        1 aaaacttact agataatctc caaaacttat ttaaagaata aggggttttt atgacagata
       61 aaactatcaa agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac
      121 tgaggaaatt acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag
      181 aagcagtaac aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg
      241 aattacccga ttcattacct accgattacc tactcaagga gattgaaact aagaataatc
      301 atattgaaga aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa
      361 aattactcga ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc
      421 acgaacaact ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta
      481 ctgaagaaac tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac
      541 ccaacaattt ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat
      601 agcgacatga tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc
      661 ttacgaaagg tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca
      721 ctttttcgtc attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat
      781 actatatttg tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac
      841 attttagttc ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa
      901 ctcacaacaa aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa
      961 aacgattttg accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa
     1021 aaatttatcg atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac
     1081 aaccaactca ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa
     1141 acacgttacc ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc
     1201 agatatcaat aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac
     1261 taatacgaca actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat
     1321 tatgtccgaa atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat
     1381 cggtacaaat tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc
     1441 ctcccgacga aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag
     1501 aaattttgac ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt
     1561 tctcagtttc ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg
     1621 cacaaaaaaa gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc
     1681 caatctccaa atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg
     1741 aaataaaaaa agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga
     1801 aaaagcagcg cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa
     1861 gtttctaaaa aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa
     1921 tcaagcttcc gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg
     1981 atcccttggt acaggcgtta cttggtagta tacacattat gttaactaac gctataaata
     2041 acgattatgt tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt
     2101 gtatatttta attataggtg tataccactt aaatttaaca caaaggcgtg attattttga
     2161 aacccaaaaa gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta
     2221 taggaattac aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa
     2281 ttgaagaaca agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc
     2341 ttttaatgga aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa
     2401 tgctcgatgg agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg
     2461 ataatacgat taaatatatt tataccgtca aaaaaaatac aaatggagaa tattcaggaa
     2521 gtatttcaca atttttagca aaagaacttc aaaatctagt agatagtaat ataacagata
     2581 caaccttatt ttttcaagat aatagcattt attattccga taatggacaa attaaactac
     2641 tttggaaatc acctttaagt acagaaaaaa atggtgtttc ttcccttaca gaaaaaaatg
     2701 atcttatatc cgtaaatata aaaaataaaa ttaaaaattt ttctaaaaaa caaaattttc
     2761 ctgcggttaa acgtgtaaaa agtgtctcct ccggagataa ctttattatg attaattggc
     2821 gtatagcaga aacccaaaca agttttccgt ggtgcgctgc ttacgtagaa gctgctatct
     2881 tatgtaacaa aacagatgta cgtgctactt cagctagagc cattatgcat tatacctatc
     2941 ctaatttaaa caacagtcaa attgaatcaa aaagtcttaa tcaagatcaa ataataaaat
     3001 atgcaaatta ctgtggtgtt tatcctaaaa aaatagcacg aatactaaac ctttcagagg
     3061 tccaaacaca actaagaaaa ggaaatgcca tttatatggg aactgctggt tatggaaatt
     3121 atagtaacag ccgtcatgca cttgcattat ttgggtgggt caatcaaggt ggttatcaaa
     3181 cttactatgt gtggaatcct tggtataact acccaactgt aataagtgca aatgcaggac
     3241 cagttacaat tcctgtacct ggaggtggtt ttacttggga caataccatt acaaattggt
     3301 aaataaactc attgctttag ttatgggtct agtgttagtt cttttcttaa ctagttgttc
     3361 aaaaaaacaa gaaatacaaa agactgatct gattcctttt ctaggaactt ggcaaactaa
     3421 agatcattca gaacgtctag taatcactaa tttaaatact tcaaaaaata tagaacaata
     3481 taccatctta gtaaaagaga acaatcataa aattcaaatc attgataaaa atttaaatat
     3541 aaatgaacaa gttttcattt acaaagcaac aaataattta ctaacactgc gctcgaataa
     3601 aaaacaatta gattacactt ttagtttgga ttctccaaaa aagctcgtat ttattttgac
     3661 aacgaatgat gggacagaag ggacagctaa accaattgaa ttttttaaag ctgattgatg
     3721 aatagttgtt tgatattaca aaacaataat ttcaccgaag gtgaaacaag cgaaacttta
     3781 caaaaagtgg ctagcatttg ctagcctttt ttgtattgtg gagcgtgggt tttgaggggg
     3841 attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcaccaaat acgtaggtat
     3901 ttggctagca aatatagagc ttgccaagcc ctttagtgtg ttttgtagaa agggacctta
     3961 tagtcacttt cacattacaa acgatacgtc cataggacgt atcaaaaggg tgggacttat
     4021 gagcaaatta aagcaaaatt tggctagcaa tccttctaaa aaatacacct attgatcaga
     4081 gtcaaaataa atcagttttc gagtgagcga atccaaattt gcaactctga agctttgcaa
     4141 atgagtgtgc cggcttccgt aaaagtctag gtaacattag tcgatatata catagggctc
     4201 taatttgcgt tttaaggcgt tttaaacgtt ctggtatgta attataccaa agcgacttta
     4261 aaacgcctta aaacgaattt tagaggttct gtagaacgtt ttagggttgt tagggcttgc
     4321 cctgaccgcc tgtaaggcgc tttaaaaaca agataaggga ttttatctag acttgtctta
     4381 aaacgccgta gatgagcgct cagagcttct aaaaggactc cttacgaact gtttttaagc
     4441 aaaggagcct ttaggcgact gcgcagttta atgtgagagc gtagcgatca cacctttaaa
     4501 gcgaccgtag ggagcagatt cccagtttag gaaatttcgc gaacctgttt ggagaaaaaa
     4561 agtaggaaaa ttttaggcgg gaaattttgc ccccctcttt tataatctct tttataacct
     4621 ctttttaaac ctctttaccg tcggctgtat ccttactctc ccaaggtgtg cagaatcttt
     4681 taggggtctt tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttttgtc
     4741 tgtttttagg ggtctttttg tctgttttta ggggtcttta attagggggt tgacccccat
     4801 tagaaagctc tttataattt tgttaagtag ttaatcttga aggaggattt tatggcaaat
     4861 gaaattgtga aataccacca tgaattaaat acgattcctt tacgcaaatt tacgtcagta
     4921 gaaatgaatt tattcttctc tataatatct agaatgcgtg atgtaggcga taaaaaagta
     4981 caatttacat tcgaacaatt aaaagattta agcaattata aagcaactgc aaatgttcga
     5041 ttcatagatg atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga
     5101 agtgctgacg gtttgcaacg tgaacgattt gttttattta atcaatttaa aatcgatgga
     5161 aaagctgata ttccttttgc tgaaatacaa gtccatgaga aagccttacc cttacttaat
     5221 aatcttgaag aatgggttcg atattctctc caacaattca atgaattaga aagcagttat
     5281 tccaaaacca tgtttagatt attaaaacaa tacagaacca aaggaattgc caatttttca
     5341 aaagaagact ttcatgaatt acttgatatc cctaaaagtt atagagaaag tgatataaat
     5401 aaaaaaatta taaagcctat tcgccaagaa ctaaccgcaa tcttcaaagg tttgacaatc
     5461 caaaagagat acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa
     5521 cctgagataa aaaaatcaga cgactttaac aaacatgctc caaccaaaga agaacaaaat
     5581 aaagctttgg aactagctaa tcaacaggtt caatctgaaa aagaacctac ttcattacaa
     5641 gaaaaaatca aagaacaaaa acgaaaatta gaacttttac aacagttagc agaattagaa
     5701 agaaaacaaa ataccccaag taagaaaatt aacaaacaaa tagcagataa caattccgat
     5761 attaccccag aatttaaaga a
//
LOCUS       DAYYFD010000130         5769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000130 DAYYFD010000000
VERSION     DAYYFD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.134"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHA25_002693"
     CDS             complement(<1..300)
                     /locus_tag="ACHA25_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8763934.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHA25_002694"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHA25_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHA25_002695"
     CDS             complement(1014..1304)
                     /locus_tag="ACHA25_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763935.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHA25_002696"
     CDS             1706..1942
                     /locus_tag="ACHA25_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763936.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHA25_002697"
     CDS             1949..3901
                     /locus_tag="ACHA25_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8763937.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHA25_002698"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHA25_002698"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8763938.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACHA25_002699"
     CDS             complement(4797..5489)
                     /locus_tag="ACHA25_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8763939.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1893 a          997 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttccagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DAYYFD010000131         5727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000131 DAYYFD010000000
VERSION     DAYYFD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.135"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACHA25_002700"
     CDS             196..921
                     /locus_tag="ACHA25_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763940.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACHA25_002701"
     CDS             911..1420
                     /locus_tag="ACHA25_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763941.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACHA25_002702"
     CDS             complement(1490..2938)
                     /locus_tag="ACHA25_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8763942.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACHA25_002703"
     CDS             complement(2938..3654)
                     /locus_tag="ACHA25_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8763943.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACHA25_002704"
     CDS             complement(3635..3985)
                     /locus_tag="ACHA25_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8763944.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACHA25_002705"
     CDS             4129..4902
                     /locus_tag="ACHA25_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8763945.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHA25_002706"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHA25_002706"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAYYFD010000132         5578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000132 DAYYFD010000000
VERSION     DAYYFD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.136"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..470)
                     /locus_tag="ACHA25_002707"
     CDS             complement(<1..470)
                     /locus_tag="ACHA25_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8763946.1"
                     /translation="MNDFTTEILKTLANKGDLNGLFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDK"
     gene            927..2438
                     /gene="gadC"
                     /locus_tag="ACHA25_002708"
     CDS             927..2438
                     /gene="gadC"
                     /locus_tag="ACHA25_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8763947.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            2451..3851
                     /locus_tag="ACHA25_002709"
     CDS             2451..3851
                     /locus_tag="ACHA25_002709"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8763948.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(4016..5451)
                     /locus_tag="ACHA25_002710"
                     /pseudo
     CDS             complement(4016..5451)
                     /locus_tag="ACHA25_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1724 a          931 c         1063 g         1860 t
ORIGIN      
        1 tacttatcat ttagagttct aactttaaat gcatttactt cttcagtcag cactttagtc
       61 atgttggaca tggtttgggg cgtgtagtga tgcccataca ttttctcaat caagtctgcg
      121 atttccgaca tggtaatacc ttttcggaag aggtgaatga cggtctcctc taatgtgtca
      181 ttcgtccgtc tataagcagg aacagtctgt tgcttgaact cgccattgcg gtcgcgcgga
      241 atctggagat gaagttcccc atactcggtc ttgaccgtac ggtcatagga gccgttacga
      301 gaattacccg tgttaaaacc aatgcgatcg tacttttcgt aatcgaggaa agccgttaac
      361 tccgttttga gaagcgtatt gacagctttt tccaaatgga cacggaataa tccattcaaa
      421 tcgcctttgt tcgctagagt ctttaggatt tctgtagtaa aatcgttcat atggaagtcc
      481 tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat
      541 ttacacaaaa tattttacac tctcaacaaa agtatcttcg attaatactt gctcaagtta
      601 tgtgcagcta cgaaaaagta ggtaccgagg tactttcttt ttcgcagctt tttttggact
      661 tatgagaagt acattcgctg gaagaatacg atataaaacg agctttttca ctaaagatat
      721 taatagagtg aaagaaacaa gcttttgttg tcagtttttt cacttttcta aaaatgaaaa
      781 taaaaaattc tggaagtgga gaagaatttt gcttgctata ctttgtcttg tagaaaagag
      841 gtagacaaaa taatgcaagc tcaaataagc gattttccat aaccttggta ggaatgacaa
      901 attaaatagg aaagggaagt aataatatga ataaaaaaaa gttatctcta ttcggttttt
      961 ttgccctgac agcttccatg gttttgactg tttatgagta tccgaccttt gcaacatcac
     1021 aattacattt ggttttcttt cttcttttgg gaggtatttt atggtttcta ccagttgctt
     1081 tatgtgctgc agaaatggca acagttgaag gatggaaaga tggtgggatt ttcagttggg
     1141 ttagtcaaac tttaggagaa cgttttggtt ttgcagctat ttttttccaa tggtttcaaa
     1201 ttacagtagg ttttattaca atgatctact tcatactagg agcgctgtcg tatgtaatta
     1261 attttccaga gcttaataca gatccattta tcaagtttat tggtgtatta gtcattttct
     1321 gggtactgac tttttcacag ttgggcggga ccaatcgtac cgctaaaatt gctaaagcag
     1381 gatttgtcat tggaattgtt attccttcga tcattctctt tggtttagca gcagcctact
     1441 ttatcggcgg taatccgatt caaatggcta ttaatgcgaa agcttttatt cctgactttt
     1501 ctcagctttc aactttagta gtatttgtat cttttatatt ggcttatatg ggcgtagaag
     1561 cttcagcatc acatattaat gagctggaaa atccccaaag aaactaccca ttggcaatgt
     1621 ttttactagt gattttggcc atcgccttag atgcaatcgg cggtttctca gtagcagcgg
     1681 ttattccaca acatgacttg tctctcagtg ctggtgtaat ccaaacattt gaaacgctta
     1741 ttttacattt taacagtcat ttacattggt tggtaaaagt gattgctctc atgattgcct
     1801 gtggcgttat gggtgaagtt agttcttggg tagttggtcc atcacgaggg atgtttgcag
     1861 ctgctcagcg aggtctgtta ccagttttct tccggaaaac taataaacaa gacgttccag
     1921 ttccgttgat tatgatacaa ggaattatcg ttactatttg ggcagcagtc ttaacttttg
     1981 gtggaggagg caacaattta tcattcttag tagcaatctc cttaactgtt gtcatttatc
     2041 tcgtaggata tttactattt ttcctaggtt actttgtatt agtttttaag aaaaaagact
     2101 taaaacgaac ttacaatgta ccaggaaaaa ctgttggcaa aacgattatc ggtggaattg
     2161 gttttatttt atcagtgttc gcattgctta tctcttttgt accaccagct tcaattgcag
     2221 ctagtgagca ccgaacatac caaatcatac tacttattag ctttgtagtg actgttcttt
     2281 taccttttat catttatgaa gtacatgata aaagtgctca cacaactatt gaggaaccta
     2341 ctcacgtgaa agctgaagac gttaatcccg ctgtttatcc agctgctaga ggagagcacc
     2401 acgttacaaa aaatgatgaa catattttaa aacattagga ggaattcatt atgttatacg
     2461 gaaaagataa tcaagaagaa aaaaactatt tggaaccaat ttttggctct gcaagtgagg
     2521 atgttgactt gcctaaatat aagttaaaca aagaatccat tgaaccacga attgcttatc
     2581 aattagtaca agacgagatg ttggatgaag gaaatgcgcg attaaactta gctacttttt
     2641 gtcaaacgta tatggaacct gaagcagtga aattgatgac ccaaacgtta gaaaaaaatg
     2701 caattgataa atcagaatac ccacgaacaa cggaaattga aaaccgctgt gtaaatatga
     2761 ttgctgattt atggcatgct ccaaataatg aaaaattcat gggaacttca acgatcggct
     2821 cttcagaagc ctgcatgctg ggtggtatgg ccatgaaatt tgcttggcgt aaacgtgctg
     2881 aaaaattagg tcttgatatt caagcaaaaa aacctaacct ggtgatctct tctggttacc
     2941 aagtttgttg ggaaaaattc tgtgtatatt gggatgtgga actgagagaa gtcccaatgg
     3001 atgaaaaaca tatgtcaatt aatctagata ctgtcatgga ttatgtggat gagtacacaa
     3061 ttggtattgt aggtattatg ggtattactt acactggtcg ttatgatgat atcaagggtc
     3121 tgaatgattt agttgaagct cacaataaac aaactgacta taaagtatac attcatgttg
     3181 acgctgcatc gggtggcttt tatgcaccat ttactgaacc tgatctagtt tgggattttc
     3241 aattgaaaaa tgttatctca attaattctt caggtcacaa atatggtttg gtatatccag
     3301 gtgtgggttg ggtcttatgg cgtgaccaac aatacttacc agaagaatta gtatttaaag
     3361 ttagttactt aggtggagaa atgccaacta tggctatcaa cttctctcat agtgcagcac
     3421 aactaattgg gcaatactac aactttgtac gctatggctt tgatggttat cgtgatattc
     3481 accaaagaac tcatgatgtt gctgtttatt tagccaaaga gatcgaaaaa actggtattt
     3541 ttgaaatcat taatgatgga tcagaattgc cagttgtgtg ctataagctg aaagaagatc
     3601 ccaatcgcga atggacacta tatgatttat ctgatcgtct gttaatgaag ggatggcaag
     3661 tcccagccta cccattacct aaagacttgg atcaattaat tattcaacgc ttagttgttc
     3721 gagcagactt tggaatgaac atggctggtg attatgtaca agatatgaac caagcaattg
     3781 aagagttgaa taaagctcat attgtttatc ataaaaaaca ggatgtgaaa acgtacggct
     3841 ttactcacta aaattcttca gagaaatata aaagaagtta attaactaat aaaaggagtt
     3901 gtgattacca cacagctcct taaatctatt tgaataaata tatgatattc atttgttaca
     3961 ctctcaatct tagctgttgt tctattcatc attactctta ttgtaagtag ttcagtcaaa
     4021 tttgcttgtt caattgatcg ataacagtat gaattgtttg aaaagtaatc ggatatataa
     4081 ttgttttctc actacctaag ccttcttggt ataaagaact atcgcaaagt ataatccgct
     4141 gagtacaaaa gaaagtgtcg tctactattt ctctaagttg tttacaaatg atattactaa
     4201 tagaaaagtg ttctcccaaa atgtcttcta actgttgtcg atagtataaa tacctaaagt
     4261 ctgacggagc tactatgaac acttcaattg gagatttccg tttatgctca tttaaaagac
     4321 ttaaactaat ggttagttta cgaatagaat tctcattcca acgcagaaat aaattgtttt
     4381 gttttcgcca ttgttcaaga atttcatgaa cttctaagta caaagcgcgt tgctcttcat
     4441 ttaacaagta agtcttatca ggttgaaata tctgaatatc agcccaaagt gagcggataa
     4501 acgggagaag tgctttatca aaaagtatct gattttcttc agatatattc agagataaga
     4561 taagtttttg actgagctca atcaccaaag ggttatctgc tacatggttt tgataaacaa
     4621 cgttcaagtc tttttgtaac aattcaaagt tatcgcatag ataatttcta gagttgaata
     4681 gaaagtagat ataaaggcat tcatcttcgt catgacataa agagaaagaa agttgctcta
     4741 atcctttctg tattagctga aacaagggag tatgtcgggc aactcttttt tcttcctgac
     4801 taaaatatat tgggttagtc ttagatctct gcaaaccgat agcgatacct aataaaataa
     4861 attgtttttc aactgtaggg taacttcttg aaaaaaatgt ttttctacat aatcaatcaa
     4921 ttgttgaccg ctttgaataa ttgatttatt tagctggatc aactctgtcc gatttgtctc
     4981 atagattatc gttagtaaca aacttcgata atccttttca ggaatttcaa aatggccatc
     5041 agcatttaag tactgcattt gtttaaaaaa attaatcaac tcatttcgta agttgtaaac
     5101 tttgcttaac gaaatgaagg tagtatcagc tatattactc caattgtgtt cccctgataa
     5161 ataatgtcct agtacattga aaaagtcaga gcgctgatag atgagttgag tgagtgccaa
     5221 cctcgaaaac gaagagtcga atgcgactgt aaagctactt ctagatttct ccactgaaat
     5281 aatttgctga tggagtatgt cggtaatgca ttccaagtcg ttagtgacag tgagtgggca
     5341 aacgttcaat tgatctgcta tttcgcgaat ctgaatggtc cctttttcgt gtaattgctc
     5401 acaaatgtaa acttgacgaa aaatactttt ctccagatag gtttccaaca tagtactcct
     5461 cctagtcatt ttatacataa ctattataac tctaccaatc ggaatagtcc tgtaatatat
     5521 tgaatcaaaa atataaatta taaaataatt agatggggta tttggtttaa gtatccct
//
LOCUS       DAYYFD010000133         5486 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000133 DAYYFD010000000
VERSION     DAYYFD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.137"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..475)
                     /locus_tag="ACHA25_002711"
     CDS             complement(<1..475)
                     /locus_tag="ACHA25_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM8763949.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIV"
     gene            complement(480..665)
                     /locus_tag="ACHA25_002712"
     CDS             complement(480..665)
                     /locus_tag="ACHA25_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8763950.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1019..1264)
                     /locus_tag="ACHA25_002713"
     CDS             complement(1019..1264)
                     /locus_tag="ACHA25_002713"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763951.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(1258..1659)
                     /locus_tag="ACHA25_002714"
     CDS             complement(1258..1659)
                     /locus_tag="ACHA25_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8763952.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1846..3654)
                     /gene="uidA"
                     /locus_tag="ACHA25_002715"
     CDS             complement(1846..3654)
                     /gene="uidA"
                     /locus_tag="ACHA25_002715"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8763953.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(3677..5443)
                     /locus_tag="ACHA25_002716"
     CDS             complement(3677..5443)
                     /locus_tag="ACHA25_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8763954.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         1719 a         1078 c          689 g         2000 t
ORIGIN      
        1 gtacaattgc ccttttaaaa actactctca acttattgaa tatctgttta atataatttg
       61 gtgataatgg ttgctcaatg ccctttttta caacatggat tttcgataaa aactgttgaa
      121 attcctgtat atgaattggt cgaatcttta caacctgcat ttctccaaaa aaatccaatg
      181 cacgttgtaa aatatgatct gttttcatat atgttttttc tacagttccc aacttatacc
      241 aaggaaaaaa atactctcga taaaaatcat gaaacgttat tccttgcaga taaattcctt
      301 gtgaatcaga aaaactttgt tttaactctt cgagtcccct tctagcttct ccttgagttt
      361 tgaagcctct tctggtcact tgaattcttt ttccaaagcg atcttgtcct aaagttgcag
      421 aaaaaaacca agtgcctctt tttctgtctt tataaacatt cgcaattttc ctcattttac
      481 tattcctccc aagaaatatg gagaacttct tctaaaattg aaataggaat tctttttatt
      541 tttgaattac gataaaaaaa gtatcctttc tcaactaaaa gttctttaca ttccttgaag
      601 attttattag catagtactg agaaaaacct agattaatca aatcttttcg agtagctgtt
      661 ttcattatag caccttcctt actttttttc atttatttct atcagctgct tttgtcaaaa
      721 gcacatagga atttcacctc tccccaagtt tacggcgagc cttacggaag tatcattatc
      781 ccaactttaa atcatcgctt tttattgact ataaaaatta ttttagaata tttatcgctc
      841 cttctttaca gcaagaaatc atggcgcaac taaaatatgc tccttaaagt gttaacgtac
      901 tgatagaatc tatttaattt tcaatgaaca ataggttttc tacctaaaag aatctactaa
      961 ttccttaact attagaagat tcttttaaac agaaaaagtg gaaaggggat ttttttaatt
     1021 agttaatttt aaattttaag atcgcttcta ttaatttaat ttctagctga cttatcatat
     1081 attcatctac ataggtaacg aattgaccta cctcgttaaa tccatttgtc aaacataact
     1141 tagccatata agaatgataa tgtttgagta tcatcttgat cgaaattgga tctccttcta
     1201 gagcagataa gataatggat agtggaacta ataggtactg ccgcttttta gatttatcta
     1261 ccataggtca taccccattt tcaattgttc aactgccttc tttcgttgat accagatacc
     1321 acttgtgctc atattgagaa ttttaccgat ttcagcatca ttaaaacctg caaaataata
     1381 aagtaggata atctttcttc ttatctccga taactgatca attgctgctg ataacttttg
     1441 atctgaaatt aatatttcca ttccaaataa gaaatagaca tcttgttcat caaaatgagt
     1501 ttgggaatac ctgccttcat ttgaatctgc taatggttcc tcattatcct ttatgttagc
     1561 gtttcgtttt cgaatagact tagcttcgtt tcgtaatact tttttacagt aggcatcaaa
     1621 ttggtgttgt atagatatgt cattgtaatg ttgttgcatg ataatatgct cctttttccc
     1681 cctttccact aatacaacaa tcagaaagcc cctttttttc gaaaaaacta gaaaaaaaac
     1741 ataaaaatat ttatcattct ttaaacagaa aatctttcta taaaaaacaa aaaaaaacga
     1801 ttaggctagg ctaatcgttt tttgaacatt cttaaaaaat ccaaattatt ttttataact
     1861 gaaattgggt attgatgacc atcgtttctg tagatagtaa gcaatcattt taggttttct
     1921 atctctagta aagattcctt tcttatttcc ttgcactctt aaaatccctt gacttgttgc
     1981 aaaatctgca aaattccaca cttgttcacc aacaaaattt tcacatgtat ctattacttc
     2041 gtgatttgct tcgtaataag ccacttgata ctcttctgta aacattgttg gtactgtatc
     2101 atgaaatcca gctactgtat ctgctccata ttctgtaaac attattggcg tatcaggaca
     2161 tctttccatc catccatcta gctctttttt caacagatat tttgcatgtg ccagatcccc
     2221 tcccgaaaca taccaaccat aataacgatt taaacataaa acatcgacaa tatctcctac
     2281 cttacaaaca tctggcgttg attttagata agtaacgatt gttacaggtc ttttctgcgg
     2341 atcaagttct ttagttaatt caactaaagg ttcaaaatat tctttagcac cttcggaatc
     2401 cgaatctggt tcatttgcaa tagaccacat tacaacactt gggtggttct tgtcgcgttc
     2461 tatcaattcc ttgatcaccg ttttatgaga attttctgtt cgtatctctt tccacgtatt
     2521 atgtttttcc acattatcat ctaataaagt ggccatgaaa tttaaatgta accctactgc
     2581 aggtacttca tcaataacta caataccttc tcgatctgct aatcgcataa tttcttctga
     2641 gtaaggataa tgtgatgttc taaaagaatt agcacctatc catttcatta aattaaaatc
     2701 tttaatattt acagcctcat tcaaaccacg tccattaaca tacgaatcct catgcttacc
     2761 aaatccttta aaataaaaag gtttattatt aatcaaaaat ttgccattgg taacctgaac
     2821 actgcgaata ccaaattctt ctgtataggt atcgatcact ttattatcat caattaaact
     2881 aattttcata ctatataaat aagcgtctag aggttcccaa aaaattggat tcttaagctg
     2941 aatcattcct ttctttcctt cagcttcagc tacaagttga gcctcttcgt catatatcgc
     3001 tattaagatt tttccggtcc attctgtatc gatattgaat tctacacttg catccaaatc
     3061 agttattctt gtattaatag taatatcact tatatagtct ttcggagttg tatataactt
     3121 aacaggtcta tgaatacctg cataattgaa gaaatcaaaa ttaactaagt tttccttttt
     3181 tatatcccca ttttcctgta ctgtttcctt cataatcccc acaggtaaag tactatcgtc
     3241 aataatattg tttacagcaa ctgtaattcg attattcccg tcttttacaa accgattaat
     3301 ttcagactca aatggtgtaa acccaccttt gtgttgagta ataaactttc cattcaaaaa
     3361 aacttttgct tcatgtgttg cagatccaaa acgaagaact aatctttctt ctaataatct
     3421 tttaggaaga gtcacttctt tttcatacca cacccatcca acatgatttc taatgttctc
     3481 tgtttcaaat aaatcattat aagaagaggg aactgaaatc ggataactct ctttttcatt
     3541 tattggttct aaagcaagtt cttcctgtaa gtcatcattt gtatctaatt taaatctcca
     3601 gagaccgctt aagtcaatta cttgcctcgt ctctgtcata atgggataca acatataaac
     3661 cactcctatc taaatattaa ataaatgtat cctttaaacc acaataagca tctattggac
     3721 ttactccctt aaattcagaa agacccatta gtttgtccac caatatttct aaagtttctt
     3781 gttttccatc ataagtatta atataagtct ttacttgagg tacatcagcc aaatgaaacg
     3841 gacactgtac agatacaaaa attgttggaa tttcatgaat gtaaaacggt atatccggag
     3901 ttcctttact tgctggccag acaattcgtt gaactgtgcc tggattaaca ttagctatat
     3961 taatgatcaa gtcatatttt ttagttaaat ttttaattgg tctcttagca gcatatattc
     4021 cagctatatt tttaattttt tcttcttgcg ataatccttt caattcttct tcaggtgaaa
     4081 tgtatttttc aacttcaaat ccttttgctg acaaaatctg tttcaataaa tcaatggctt
     4141 gaggtctatt tccagcaact acttcaccaa acccaccttt taccccatgt acatctacta
     4201 gtaaaatcct tttatatttt tctggtacaa taggccaaat atcttgttta tctttaacta
     4261 atgtaattgc ttggtcagcc acctctttaa aaattgattt attttcaatt aaaccaattt
     4321 tttccagagc ttcttgctta ggtaataaaa tttgttcttt tgcttttttg tgtaatccca
     4381 atttagcttt taaccctaaa attcttttta aggcatctaa taaccgatca tcagtgataa
     4441 ttcctttacg atagccttcc atcatataat taaagtcttc atctggatca ttaaaaaata
     4501 gaaatagatc acatccagct gcaatagaag tgggcaataa ttctgatcgt ttcattgccc
     4561 cagtcattcc taacatatga ctagcatcag taactactaa tccattaaat ccgagttttc
     4621 ctctaagtaa gtcagtgatt aattcttttg ataaagttgc aggaagtaaa tcttcatctt
     4681 tcagatcagg attaaaatgt tttgaataag ctggcatatg gatatgacct gccataattg
     4741 aaggtaatcc agcttctatt aagcctgagt atacttttcc aaaagaatta tcccattctt
     4801 ccgtacctaa actattaata ctaaaagata gatgctgatc tcgttcgtca ataccgtcac
     4861 cagggaaatg tttagctgca ggcaaaattc cactctcctg aatacctttc atataagcta
     4921 aactaagttc taaaacttta tcagggtttt gtccaaaaga tcgactagaa atgataggat
     4981 ttctccaatt tctattaata tctacaatag gagcaaaact ccagttacat ccaatagccg
     5041 atgcttcaat cccagaaact ctccctaatt cataagcata tttcgcatca tttgttgcac
     5101 caatttttac ttctacacca acttcagtac catctgtaca agctccgtta ccacctgctt
     5161 ccgtattagc agcaattaat agaggaattt tactttctgt ttgtaagata tagttttgat
     5221 catatacctc atcagcagtt cctggattat atcgtacagc tccaatatgg tactgattga
     5281 ccatattact caagtaattt ttatcacgac tagatcccat attaacaaac aattgaccta
     5341 ttttttcttc caaagtcata gaatcaatcg tctcttcaat ccaagaaata gcatcttcat
     5401 ctaaattata tggttttttt cgtaaatcta cttttatagt cattttcttt ctcctctact
     5461 tattagtaag atttttccaa aaagta
//
LOCUS       DAYYFD010000134         5355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000134 DAYYFD010000000
VERSION     DAYYFD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.138"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..1813)
                     /gene="glmS"
                     /locus_tag="ACHA25_002717"
     CDS             complement(8..1813)
                     /gene="glmS"
                     /locus_tag="ACHA25_002717"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8763955.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2223..2513
                     /locus_tag="ACHA25_002718"
     CDS             2223..2513
                     /locus_tag="ACHA25_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763956.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2526..3551
                     /gene="sppA"
                     /locus_tag="ACHA25_002719"
     CDS             2526..3551
                     /gene="sppA"
                     /locus_tag="ACHA25_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8763957.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3554..4213
                     /locus_tag="ACHA25_002720"
     CDS             3554..4213
                     /locus_tag="ACHA25_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8763958.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4370..5302
                     /locus_tag="ACHA25_002721"
     CDS             4370..5302
                     /locus_tag="ACHA25_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763959.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
BASE COUNT         1684 a          960 c         1124 g         1587 t
ORIGIN      
        1 tcttttatta ttctactgtc actgatttcg ctaaattacg tggtttgtca acgtcgaatc
       61 cccgtaatag cgttgcgtaa tatgcaagta attgagtcgg tacgactgtg gttagagtag
      121 ttagataagg gtggacagtt ggtagcacca gctggtcact ttccttttct aatccctctg
      181 agacaataac tagtgtttta gcgccacggc tttcgacttc tttcaggttt cctcgagtat
      241 gcgcaccagt cacttcttca ctgatgattc caatcactgg tgttccttct tcgatcaaag
      301 caatcgttcc atgtttcaat tcgccagcgg caaaaccttc tgcctggata taactgattt
      361 ctttcaattt caaagcagct tcaagagaaa cgttgtagtc agcgccacgt ccaatgtaaa
      421 aggcatttct tgtatcgctg agatatactt ctgccaactc ttctaaataa tctttttggg
      481 cgatgaccga ttccatcgca ttagcaacta aactcaattc atgagtgata tcgaatacaa
      541 gagaagtttc aaattctttt ttatcaccga tagctttggc taataaagtt agtacagcta
      601 tttgtgcagt ataagctttc gttgatgcta ctgcgatttc gggtccagca tgcaatagca
      661 gtgtaaagga tgcttcccgc gaaagagtag aaccagcaac atttgtaatc gtaagtgaag
      721 ggtagccgag acggttgatt tttaccagaa cttggcggct atctgctgtt tcgccacttt
      781 gactcaagaa aataaagaat ggtttttttg aaagaagagg tgtgttatag ccaaattcac
      841 tggctaaatg aacctcaaca gggatctgtg tcaatttttc cagagtctgt ttacctgcta
      901 accccgcatg gtaactagtt ccgcaagcaa cgatatagat tcgatcgcta tcagacaatg
      961 tatcgaccag cttttgatca agagagacac gattgtcttc tgttacgtat ttttgagcga
     1021 tacgacgcat gataataggc tgttcgtcga tttctttcag catataaaaa ggataggtac
     1081 ctttctcgat atctgaagaa tctacttgtg cttcgaatga agaacgttct acgatgtcac
     1141 ctgcaaaatt ttgaataata actttttctg atgtgacggt aaccatctct tcatcttcaa
     1201 tctcaacgaa ttccttcgta tgagcaagca tagccattgc atcgctggca atcacattga
     1261 atccatcgcc gagtccgatt aatagtggac ttttattttt agcgacgtaa ataacatcag
     1321 gtgctgtccg atcaatcaaa gcgaaagcgt aagaaccttt gatgacccgc aaagctttta
     1381 aaaacgcctc ttgcgttgta gtatccttcg cgaaagtttc aattagatga gcgataatct
     1441 cagtatcggt ctcaccaata aagtggtcat ttgctaaata agcaatttta agttcttcaa
     1501 agttttcgat tacgccattg tgaacgagca cgaagcgtcc actttgggaa gtatgtggat
     1561 gggcgttttc ttcagaaggt tgcccgtgag ttgcccagcg tgtatgacca atacctatat
     1621 ttcctgtagt atcaatattt attttgtttt tcaaattttg aattctgcct tgtgctttga
     1681 ccagataatc agttgtgcca tctgagacaa agataccagc tgaatcataa ccacggtatt
     1741 ctaatttttc tagaccgtca atcaatcctg gtgttacatt ttttaatcca atcattccaa
     1801 caattccgca cataaaaaac atcctacttt ctataatgaa ttcgtatgaa tgcttccagc
     1861 tgcaggaaaa tccttttgtt ttatctttgc agatattttt tgtggatttt ctgtagagtg
     1921 cagtccctag ggccatccgc cgaatctttc gataaaccct ctcctcgtca tctgtttaca
     1981 cagacctggc gctatcctaa aaggtataac tatcctcctc ttttgttttt tatctggaaa
     2041 catttaaaat cataaccttt tgcgaatgtt cttgtcaaca aaaaaagaat aaaaaataaa
     2101 ttggtatata tcaaatgctt tgtcaaatgc tctttttatg tcaaaaaata atgggttttt
     2161 atctatcatc tttttcagga gttcggtaca atagaaagta atcggataga ggaggagtaa
     2221 aaatggattc aaaaattgag atcatgacac tgggaatgtt aaaaaaacaa ttgtccaaat
     2281 tcgaagcatc agctggtgta tcagatgata cgaaaatatt tttagatact ggctgggata
     2341 gcatccaaga aatcgcacct gatgccttgg aagttgctga agctcgtgag tttacggtag
     2401 aagatgaatt gacaaaagag tcgttttccg ggtatgcgcg ggaagaaaaa gcagagcggt
     2461 tcgacacttc tgagcagtca gaaactgtga tcgtcataaa aaacttgtat tagaaaggag
     2521 aaagaatgaa taagagacgt tgggcagcag ttctgattgc tgcaggatta tttgtggttt
     2581 cgttagtttc agctagtttg actaagcctc aggaaaacga tcaaattgag ggattgaaca
     2641 gttggttata tggggatgaa gaactagctt caaatgtgtt ggaagaggga gacagtacgc
     2701 aaaaaatcgt aaaattatca gtagatggaa cgattgctgc gggagggtct tcaggtttat
     2761 ttgcttctga aggttacaat cataaaaatt ttatggaaca actgaaggcg attgaagaag
     2821 atccaacagt caaaggtatc tttttagaag taaattctcc tggtggcggt gtctacgaaa
     2881 gtgcggaaat cgctaagaaa ttggatacga ttcgtaaaga acatgatatc ccaatgtatg
     2941 tcagcatgaa aaacatggca gctagcggtg gctactatat ttctgctcaa gcagataaaa
     3001 tctttgcaac agaagaaaca gtgaccggct caattggtgt gatcatgtca ggattgaatt
     3061 attcaggact tctagaaaaa ctgggtgtgg aagatacaac agttaaaagc ggagcgttga
     3121 aagatatggg ttcaggaacg cgaccagaga caaaagaaga agaagcggtg cttcaagcat
     3181 atatcgacag cgcatatagt cgttttgtag caatcgtcag cgagggacgg aataaatctg
     3241 aagaagaagt caaaaaaatt gctgatggaa ggatttatga tggtgtccaa gcaaaagaag
     3301 taggtttagt cgatgaattg ggatttcctg aagatgcttt aaaagctatg cgaaaggaac
     3361 aacagcttga agatgcggaa ctagtagaat attcttccac gccatctggg tttgctagta
     3421 catggttagg aagtaaacta gcagaattcc agggattgaa gccttctgag actagtcaga
     3481 tcttatccgt tcttgagaat ctagggacag cggaatcacc aaaagcaatg tattattacg
     3541 gaggtgagta agcatgtcag agctttcaaa tgaaaataag gaacaagaaa aagataagca
     3601 acagcagcct gctgtaggac ctgactccaa cttttcccaa aaggtactga gctcttttga
     3661 aagagagcct ttgacgcccg aagaaatcaa aaagaaacaa cgtaaatgga aaaagtatac
     3721 ggacacaaga aatgaaccgc caaaagcaaa tgattttcct aagtatttct atgcaggttt
     3781 ttttatccga atgtttgctt tcttggtcga tcttttgtgt atcggagcta ttacgcgttt
     3841 aacacttggt attgccaaaa atctaggttg gcttaatttt tcagattctt atctgagtat
     3901 ctacggattc acagccttac ttatttatct agcgtatttt attttattga cgaaattcaa
     3961 tcatggtcaa acgattggga aaatgatctt tggtattcga gtcatcagct ttgatgaaga
     4021 agaacttagc tggtcaaccg ttttagttag agaaggtgct tgccggttca ttctaaaatt
     4081 tcctttgctg atgttagggt atcttccagc aatctttaat cagaaaaagc aacatctagg
     4141 cgattttttc actaatacaa gtgttgttac gatcaatctg atcaaagcat tcaaccagga
     4201 agtcaacagc tgatgagcaa taaaaagcag taacagttga aagcaccttc taaaagtcag
     4261 atctaaaatc tagcttttag agggtgcttc agttattttt aattgtcatg ctttttttgt
     4321 tttgctacta ttgaacatga gctgaacatg agcaagagga ggacactgcg tgaagccgat
     4381 tgattttcca gaaaaatatg agaatttaat gcgagtagca caacaagcac tagcaaacca
     4441 gcaatatcag caggcaaaag aattatttca acgagcttac gagttgaaag aaagctttga
     4501 ggctaatagc ctgcttgttt tttgtcttta cgagttggac gagaaaaaag aagcattgaa
     4561 gcaagcactc ttgcatgaaa aacagtatct tcaaaatgag gagtttgctg aattttattt
     4621 tgacttgtta atcagtacac aagatttttt atatgctaga aaattgatag caagtacaga
     4681 tttttatgaa gctttcgagc agcgggtcat tgaaaaaatc caatttgcag aagaattatc
     4741 cggacaaatg gagaggcaga agatcaaggc actccacaat aaatcggagg aacttccttc
     4801 gttggaacct gcacgtcagc ttgcgtctat tgatgagatc gaacagcttc cttatcatga
     4861 gtttatccaa acagcgagta aacttattgt atcgccagag gtccatattt tagcaagggc
     4921 aaaattactg gagacactta ggcaattgaa cgaatgccat cctgttttct atttgacgat
     4981 tgaagaaaag ttagtcaaag tcataccaaa agatctgcca aagccgcagc agcaggcaag
     5041 ttaccagcaa ttatgtgtat tttcagatcg ttacggtaat gaagatgcgc tgcttagttc
     5101 cgtgttaaat gaagaattca cgttgcaatc tgcaattgtt tatcctgtct atgatacata
     5161 catcgaagac ccgaagcgct ggtttcaact gactgtagag gcgtgtacag gaaaaacgat
     5221 gtatgaaggt acagaagatg aaaaacaaga ttttttaaaa aaaagagaaa aaattttgca
     5281 gcaaatgatc ttcttccatt agattttatc agtgaaaaag ataaaaatgt tatgaaaaac
     5341 aaggattcta tccat
//
LOCUS       DAYYFD010000135         5341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000135 DAYYFD010000000
VERSION     DAYYFD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.139"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHA25_002722"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHA25_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHA25_002723"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHA25_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACHA25_002724"
     CDS             2090..3130
                     /locus_tag="ACHA25_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8763960.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /gene="traE"
                     /locus_tag="ACHA25_002725"
     CDS             3954..4286
                     /gene="traE"
                     /locus_tag="ACHA25_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8763961.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="ACHA25_002726"
     CDS             4905..5246
                     /locus_tag="ACHA25_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8763962.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1771 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattgata
     4861 taaaacatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt
     5341 t
//
LOCUS       DAYYFD010000136         5336 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000136 DAYYFD010000000
VERSION     DAYYFD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.140"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1336
                     /gene="tig"
                     /locus_tag="ACHA25_002727"
     CDS             47..1336
                     /gene="tig"
                     /locus_tag="ACHA25_002727"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8763963.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            1583..2833
                     /gene="clpX"
                     /locus_tag="ACHA25_002728"
     CDS             1583..2833
                     /gene="clpX"
                     /locus_tag="ACHA25_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8763964.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            2916..3503
                     /gene="yihA"
                     /locus_tag="ACHA25_002729"
     CDS             2916..3503
                     /gene="yihA"
                     /locus_tag="ACHA25_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8763965.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(3577..3732)
                     /locus_tag="ACHA25_002730"
     CDS             complement(3577..3732)
                     /locus_tag="ACHA25_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8763966.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            3868..5196
                     /locus_tag="ACHA25_002731"
     CDS             3868..5196
                     /locus_tag="ACHA25_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763967.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         1852 a          892 c         1096 g         1496 t
ORIGIN      
        1 acgtttttga tgtataaaat gaatttctaa actcggaggg aattaaatga ctgcaacatt
       61 tgaaaaaaaa ggcacgaatg atggtgtttt aaaatttacg attgcgcaag acgaaatcca
      121 aaaaggatta acaactgcgt ttaataaagt gaaaaaaagc ttgaacgtac ctggattccg
      181 taaagggaaa gtttctcgtc aagtattcaa ccgtatgtac ggtgaagaag ctttatatga
      241 agatgcattg aatgctgtat taccagaagc ttatgaagct gctgtcaaag aagctggcat
      301 cgatcctgtt tctcaaccaa aaatcgatgt tgaaagcatg gaaaaaggtc aagactgggt
      361 aatcactgct gaagtaacag tgaaaccaga agtaaaatta ggacaatata aagatctagt
      421 tgttgaaaaa caagaccgtg aagtaacaga tgctgacgtg gatgcacgta tcaaacgtga
      481 acaagaagca caagctgaat tagttatcaa agaagatgct gcagctgaaa atggcgatac
      541 agtagtaatc gacttcgaag gctttgtaga tggcgaagcg tttgatggcg gaaaaggcga
      601 aaattactca cttgaattag gttcaggctc attcattcca ggttttgaag atcaattagt
      661 tggtcataaa tcaggtgaaa gtctagatgt aaatgtaact ttcccagaag attatcaagc
      721 agaagatctt gctggaaaag aagcagtctt caaagtgaca atccatgaag taaaagcaaa
      781 agaagtgcct gaattagatg acgaattcgc aaaagatgtt gatgacgaag tatcaacatt
      841 agacgaattg aaagaaaaat atcgcaaaga attagtagaa acaaaagaaa aagcagcgga
      901 cgatgcaaaa gatgaagcag cgatccgtat ggctgttgaa aacgcagaaa tcgttgaatt
      961 gccacacgtg atggttcatg atgaagtaca tcgttcaatg gacgaattct tgaacaacat
     1021 gcaacgtcaa ggtatctctc ctgaaatgta ctatcaatta actggttcta cagaagaaga
     1081 cttgcataaa caattcgaag gtgaagcaga aatgcgcaca agaacaaatc ttgtgatcga
     1141 agcaattgca gctgctgaaa atcttcaagc aacagaagaa gaaatcaatg cagaaatcca
     1201 agaattatca gaaacataca acatgccaat cgaacaaatc aaacgcgtgt tgacagaaga
     1261 tatgctggaa catgatgtga caatgaaaaa agcaatcgaa ttgatcacag gtacagcgac
     1321 agaagcgaaa gaatagaaat caagtaataa atcatttctg aagaagctac ttctagaaaa
     1381 ttgtttattc gaaataaggg actgtgacac gattttgtca cagttcctta ttttttgtta
     1441 agagtaaaaa atgcaaaggt ataggtcagt tgatcctttg ttaaacactt tttcttttta
     1501 ctaaactatg ttatgatagt aaaagactgt aaagtctttg atagtttctt gcatactttt
     1561 tgcaagttaa ggggtgaaga ccatgtatga caatccaagt agcaatgaga ccgtacgctg
     1621 ttctttttgc gggaaaaccc aagaagaagt gaaaaagatc gtagctggtc caggggtcta
     1681 catctgtaat gaatgtatcg acctatgtaa agaaatcatt gatgaagaat tttatgaaga
     1741 agctgtacgc gaattcactg aagtccctaa accactagaa attcttgaag tattgaataa
     1801 ctacgtgatt ggacaagatc gcgcaaaacg ctcgttagct gttgccgtat acaatcacta
     1861 taaacgtgtc aatcagcaag cacaagaaaa cacagatgat gttgaactgc aaaaaagcaa
     1921 tatctgcttg atcggaccta cagggtctgg taaaacattt ttagcacaaa cattagcaaa
     1981 aacattgaat gtaccttttg caatcgcaga tgcgacaagt ttgacagaag ctggttatgt
     2041 gggggaagat gtagaaaaca ttttgcttaa attgcttcaa tctgctgatt acaatgtgga
     2101 acgtgcagaa aaagggatca tctatattga tgagatcgat aaaattgcgc gtaaaagcga
     2161 aaatgtatct attactcgtg acgtttccgg cgaaggtgtc caacaagcct tgctgaaaat
     2221 tctagaagga actgttgcaa gcgtgccacc tcagggagga cgtaaacatc cacatcaaga
     2281 atttattcag attgatacga cgaacatatt gttcatcgta ggtggtgcat ttgatggcat
     2341 cgaaacgatt gtgaagaatc gtatgggcga aaagacgatt ggatttggta cacaaaacca
     2401 aaaactttct gaagacgaaa gtgtcatgca gcatatcatt cctgaagatt tactaaaatt
     2461 cggattgatc cctgagttta tcggacgatt acctgttatg gcagctttag agaaattgac
     2521 aacagatgat ctggtacgga tcttgactga gccgaaaaat gcgctagtca aacaatatca
     2581 aaaacttctt tctctagata ataccgaact tgaattcgaa cctgaagcgt tgagagccat
     2641 tgcgaaaaaa gcaatcgaac gcaataccgg tgctcgagga cttcgttcca tcattgaaga
     2701 aatcatgatg gatgtgatgt ttgatatccc ttcaaatgaa aacatcgaaa aagtgattat
     2761 cacaaaagaa gcagctgaat tgtctggtga gccaacagta atctataaag attcagagaa
     2821 aaaagcgggt tgatgaaaag aggctgccgt aaaagtcatt ttggcttttg cgacagccgc
     2881 ttgctttatt catcaccttg ttaaggagag agaccatgaa agtccatcaa gctgaaatta
     2941 tcataagtgc agtggcacca aaacaatatc cagatacaca attgccagaa atagctctgg
     3001 caggacgatc aaatgtaggg aaatcttctt ttatcaacac attgattgat cgaaagaatt
     3061 tagcccgtac ttccggtaag ccaggaaaga cccagacatt gaatttttat ttgatcgaga
     3121 acgcattaca ttttgtcgat gttccgggat atgggtatgc aaaagtatcc aaaactgagc
     3181 gagcaaaatg gggaaagatg atcgagacat atttaacgac tagagaccag ctacgtgcgg
     3241 tcgtttcttt agtagatttg cgtcataagc cttctcaaga agatatccag atgtatcaat
     3301 ttttgaagta ttatgatatc cctgtaattg ttgttgccac taaggcagat aagatccctc
     3361 gtggtaaatg gaacaagcat gagagcgaaa tcaaaaaagc gttggatttt gatcgcaatg
     3421 acacgtttat tctcttttct tccgttacaa aacagggaaa agaagaagcg tgggcagcaa
     3481 tcgaacaagc aattgaatca taaaaagaat aaaaaagact taccagtcaa actctttcat
     3541 aaaagtttgg ctggtaagtc tttttttgag cttaacttac ttttgtttct tgtctttttt
     3601 cttttcattt tgtggctgtg gttttagaaa tcgatcgatc tcttcttttg gtgcaactgt
     3661 tttttccgga tcgacagcaa atgattcgaa aagtttatct aaattatctg tacgttcaaa
     3721 tttcaacccc attgtaaaca cctctttgtt tctttttact tagtctatca tgtaatcgtc
     3781 caaattggtt tctaagacat aaagttttag tttttcttta tcgttggtta taattaaatt
     3841 ataaaaagat tttaaagagg tgagaaaatg attggcgaag ataaattatt gatgactatc
     3901 cagctcacag acttaaatga agaagaaaaa aaacaggaaa taagcgattg ggctattttg
     3961 acacgtctgt tgatccagcc aacatttatc cgctctttca cacaacaagc ggatccgtat
     4021 gatcttcgaa agcttcaaga aaaaataatg ctagcatcgg taagagacga aagttggtta
     4081 gttgtaggaa atgacgagaa cgaatgtagt tttcgcttgg aagataatca attattgata
     4141 aaaaatgtat tgtctatctc tgttttcaaa gaaaagcaat ttttgattcg agacttcatt
     4201 cagaaaaaaa tgattgctca tggggtgttc gcctatatgc gagcttatag cgagtttatc
     4261 tatcacaata caaaacagat ctctcaacgg ctattgtttg agaaaaagga tgaaatcgaa
     4321 catttaccta aaatgaaaca acaaaacggg gaagtagtag tcgattgcaa ccaatttcca
     4381 ggatacgatc ttttttacca aggtttatgt ttcacttcgt gttgggaaat gtattatagt
     4441 cgctattatc atcaaatcat ccctaaaccg atttttctcg acatccaaca agtagaaaaa
     4501 gtgaaggaat tagaaaacga agtcatatgt atccagcttt atcgagaccc ctttaactgg
     4561 caaaagccaa acaatcaaat gtttcaatcc tattttaggg accagcttgg gtttgatcat
     4621 cttgcttggg acaatggtgt cggtttattg aaaccgccat tcgttgaata catttacact
     4681 gaccatacga tccaatctgt ccagtatcag aatgcacaaa tgcaacctgt tcctaaaaag
     4741 aatgcttcat tttttgtaac aaaaagttat gatattcaac aaggagatta taaagaaagg
     4801 cgtgttcgcg gaacactaaa tgctcaggcg tattttcctt gggttgacga gaatcgctcg
     4861 cgcatgatgt gctataaagt tatcgatccg actgttgctt tggataatgg aatcgaagct
     4921 tattgttatt atattaggga atatctagaa gtagaggtaa cagatgaaaa atatcaagag
     4981 tatcttttat ccttaagaat ctatgttcct tcagaatccc tgtcagaatt accgttaaag
     5041 gaaatcaaac atagactctc tgatgttacc ttcaaacgaa taaagaaaaa gcggcgaagt
     5101 atccaattcg atgtcaagaa aggtgagcag catctgcgtg tgcaattttt agattaccga
     5161 gaattagaac aattgattac tttacaaaaa atatgaggtg aagaaaatgg ataaacaact
     5221 ctattatcga ataatggttc cggcatcatt attatccgtc tgtctctttt ttgttcctaa
     5281 ttttcctatt cgatcagtca tcggtttttt ggtcatgttt ggcgggatgg gacttt
//
LOCUS       DAYYFD010000137         5313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000137 DAYYFD010000000
VERSION     DAYYFD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.141"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA25_002732"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA25_002732"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8763968.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHA25_002733"
     CDS             complement(1297..2133)
                     /locus_tag="ACHA25_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763969.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHA25_002734"
     CDS             complement(2317..3534)
                     /locus_tag="ACHA25_002734"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8763970.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHA25_002735"
     CDS             complement(3683..3844)
                     /locus_tag="ACHA25_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763971.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHA25_002736"
     tRNA            3917..4000
                     /locus_tag="ACHA25_002736"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5313)
                     /locus_tag="ACHA25_002737"
                     /pseudo
     CDS             complement(4240..5313)
                     /locus_tag="ACHA25_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1083 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca tac
//
LOCUS       DAYYFD010000138         5148 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000138 DAYYFD010000000
VERSION     DAYYFD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.142"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..229)
                     /locus_tag="ACHA25_002738"
     CDS             complement(<1..229)
                     /locus_tag="ACHA25_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8763972.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
     gene            complement(434..2242)
                     /gene="pepF"
                     /locus_tag="ACHA25_002739"
     CDS             complement(434..2242)
                     /gene="pepF"
                     /locus_tag="ACHA25_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8763973.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2418..3416)
                     /locus_tag="ACHA25_002740"
     CDS             complement(2418..3416)
                     /locus_tag="ACHA25_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8763974.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3642..4295)
                     /locus_tag="ACHA25_002741"
     CDS             complement(3642..4295)
                     /locus_tag="ACHA25_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8763975.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4627..5025)
                     /gene="spxA"
                     /locus_tag="ACHA25_002742"
     CDS             complement(4627..5025)
                     /gene="spxA"
                     /locus_tag="ACHA25_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8763976.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1519 a         1070 c          808 g         1751 t
ORIGIN      
        1 cagacacatc attcagtaac aaccgaacgg ttgtcgtccc ccaacgaatg atcctagagg
       61 ggacgacaca aagacaatgg gaacactctt ctggcttgta cagaagagtt cctttgtaaa
      121 tcaagcaaat ttgccctttg tatttttctt tagttaaaca attttcctcg aaatagatat
      181 ttaggtctag aatatttagg gtatctttga taagctgagt atgagacatc ttgaatcctc
      241 ctatgaattg gtagccaaat taattctaaa ggatttcagg gtgtcttttc tatatttttt
      301 tatacaaaaa agatgttggt gaagttcacc aacatcaaaa agtatagagc cttaaaaaaa
      361 tacgtcgact cccttacaag agagccgacg tatttttttc gtttctattc gagaaaatca
      421 aaaatagaaa gccttataaa cgatcgatca attcttctaa ttcatttaga cgttgttcaa
      481 acatcgacat tgcatcttca atatatgcag cctgtgtcat atcaactcct gcttttttca
      541 ttacttccac aggataatcg ctgttacctg cttttaggta agccaagtag ttctctagtg
      601 cctctggttc ctggtttagt attttcttcg ccagtgctga agcagcagaa aagccagtcg
      661 agtattggaa aacatagtaa ttataataaa aatgcggtat ccgtgaccat tcgaatttaa
      721 tttctgggtc ttcttcgact gctggaccat aatattttgc attcaattta ccataactat
      781 cacttaggta ttcactagtc aatggcacac ctttttcatc ttccgtatgc atgaaatgct
      841 cgaattcagc gaattgtgtc tggcggaaaa ctgttccttt aaacccatcc aagtaatggt
      901 tgagtacata agcccgtaca cgagggtctt tttctgtttc caataaatac tccgttagga
      961 tattttcatt cgttgtcgaa gcaatttctg ctaaaaagat ggagtagtcg ccgtacacat
     1021 aaggctggtt cgaacgagtg aaataactat gaacactatg tcccatttcg tggacaagcg
     1081 taaatagctg atccagcgta tcatgccaat tcaataaaat atatggattt gtgtcatagc
     1141 ttcccgaaga ataagcaccg cttcgtttcc ctttattttc gacaacatcg atccaacgtt
     1201 cagagaatgc tttttctacg atggccatgt attcttcacc cattggtttc agtgcttcta
     1261 aagctttttc ttttgcttct tcgtacgtaa aggtaattgg agcttcccct aagaccggtg
     1321 tataaagatc atacatgtgc agtttttcca cttctaataa acgtttccgt aattccatgt
     1381 atcgatgtaa caaaggcaaa tgcttgttta ccacgtctac caaagtatcg tatacacttt
     1441 caggaatatg attattgctc aaagacgctt ctctggcaga gctgtaatta cggactttcg
     1501 ctttaaaatt atgtcctttt atatgtgtgc ctaaagtaga agcaaatgta tttctaaatt
     1561 gttcgtaaac actgtacaac cccttaaatg ctgcttcgcg cacccttcga tccgtgcttt
     1621 ctagcaactg accatacacg ccatgagata attggactat ttcaccattt tctccttcaa
     1681 tcgttggaaa aactagatct gcattattca aaacagcaaa tgtatcactt gaagcatcaa
     1741 agatttcacc tgctccagca agaagagatt cctgctcagc agataagaca tgggctcgat
     1801 tatctactat ttgctggata taatggcgat agacttccaa ttttggttct tctttgaaat
     1861 attgccaaat ctggtcatct gacaattgca atatttctgg ttcaaaccag gaaacggctt
     1921 cactaacttt cgaaaacaga ctgcttgctc ttgcataaag cgcttggtaa tctgtatttc
     1981 cagtgtcttg atcgttttta aggtgcgcat atacataaat gacttcagtt tggcgataaa
     2041 ccctcaatac gaattcaatc gcatttaaaa attgagaagc gccttgatca agtgttcctt
     2101 tgtgtttttc agattgtttt aactcttctg ataattccaa atatttctca tcaaactctt
     2161 ggtcgcttga aaagatcttg gtcaagtccc aagttaaatt ttcaggcaat tcttctcgtt
     2221 ttggcaactg ctttacttcc atgaaatcca ctccttttct tcccttagct ctatctatca
     2281 aaatagatca tagtctaaca tccacatgtc acagcatcta acttttctac tcattcatgt
     2341 gtctgcttcc atcaatttta gcataactaa atcttgtttt acatagaaaa ccgattccta
     2401 gcctattttt atacttacta ttttttcaaa gaagacaagt gtatgcattc gttaaaaaac
     2461 tgctgataga ccatctcttc atcaatcata ggaaaaagcc attccctttt atgttctttt
     2521 atttggcaaa gccatgagtt gaaatcagga actttctggt tttccaaggc ttcttcgtac
     2581 aacattcgat aaagaaaaac ctgttcttgt aaataaaaga aaaaccgact tttttgatag
     2641 atccacgatg atagatgcaa aagatgttta tgacgcaagt agcattgttc ttgtatggat
     2701 actgtattct tttgcttgtg aataagttgg cgtgtcagcc atttcttccc atcttcactc
     2761 actttttcta tttttttcat ttggttcatt ttaatttcgt tactagtgaa tatttcttta
     2821 aggtccctac tagaaaaagt ccagcagcta ctgcaaaagg aaatatgccc tgatacagtc
     2881 tctgtaatat gatgatacaa ataaagcttt tgtttcttga gatcagcttt ccagcaatgc
     2941 actcctcttt ttctattata gtagcagcaa cttttttcta tcaatgagaa ttgcgattgt
     3001 ccaagaaaag aattaccaag tatccaccat acttggtaac tgttattcaa ataattttgg
     3061 gttctttcaa taaatctctg gtgagacagt ctagaacatt ggatttctat tgctagattc
     3121 gccaacaaaa tatccggcct ttgccgcaac ctcggcaaat aggcttccaa aaatactggt
     3181 tctactgact gtcccagcca atccatgaag cattccttta actgtaagtg ctcttttgat
     3241 tctctttcac taaagaccga acagttatct gatttttgat gggcaaaatg agcacatttt
     3301 ttctttccct ttttcaaaat cactcttttt cgacagccac gacaataata tttctttcct
     3361 ttttctgcat gtttagcaaa acagcactta ttatattcat tcattgcaag cagcattttt
     3421 ctccctctga acttatttac gtctaagaat ttatttacac gaaaaaagag ggcgtgaaaa
     3481 agctattctg catcaagtaa taagaacagc taaacaaaaa tagttcccaa tatttttcgt
     3541 ttggttgggc ttgttaccgc agatgcaagc ttttgaacac atttatacga aaaaaagact
     3601 gcgacacaat ttttgtcaca gtctcttact ataatgctga tttatacaaa ataaaatttc
     3661 gttcgctcga tagcattacg cgccatcaga cattgtccat attcattcaa tgtttctggt
     3721 gtacgatcgc tcttttcagc aaactccaaa attcgagcaa tctgattctc tacatccgct
     3781 tggttgagat tttccatcca gaagtgaacg actaggtaat agcgctcttc gaataaatac
     3841 aaatcagtcc aagcagactc tagttcgaca tctgttgcaa gttggatcac ttgttcaaaa
     3901 tcttcaaaag caaaagttgc ttgtctttcc tgttcttcta gggcgcttcc agataattct
     3961 tcctccggat cgctgccaat aatctgctga cgaagaaagt cactcacatc ttctgaatca
     4021 acagtagaat cttctgattg agaaatttct tcatccggaa gatttttact gataaacagt
     4081 tctaatccgt ctccttttgg caaaacttgg aaagtgacag cttcgcttcc tcggaactcc
     4141 tcttcgatat caacttcttc caaaatacta taaaagaaat tttcgatttc tttgtgattg
     4201 ccaagtaaat ccaaaaatga aacgccccgt tcctctaaat cttcatgacc aattagtaca
     4261 cggatcgtat tttcattaat atgttccatt tccattttgc tacacctcac tttgccacaa
     4321 tgtttaatat agattcattg tagcgaaatc agcttaaatg taaagaaaaa aaatctcgct
     4381 gttaagcgaa tttaataaaa ccagcggatt tttcaacctt tcaagctatc ttatttaact
     4441 ataacatttt tggcagacga atactatata gccgactttt cagctaattt tccctctcta
     4501 tttttcgtgc aatcatcatc ttctactacc tttttataca tctaatcaag aatacttttt
     4561 gtaaaaacaa aaaaacttgg aaaaactatc tttgtaatag tcttttccaa gtttcgtatt
     4621 atccttctat aatccagcca tcaattgtgc ttgacgtaat tctaattgtc gtacatcgcg
     4681 cggtaggaac cgacgaattt catcttcatt aaaaccaact tgcagacgtt tatcatcaat
     4741 catgattgga cggcgaagca atccaggatt gttttgcaca agttccaata attcttgtaa
     4801 cggcagatca tctaaatcca tgttcagttt ttggaatact ttggaacgtg tcgagatgat
     4861 ttcttctgtt ccgtcttctg tcatttgtaa aatcgctttt aattcagaac tgttcaatgg
     4921 ttctgaaaaa atattacgtt caacaaatgg aatctgatgt tcttgcaacc acgcacgagc
     4981 ctttctgcag gaagtacaac ttggggaagt atataatgtt aacaacgtgc accactcctt
     5041 ttcgtgataa gaatcacttt tttgtaacag aagatcataa taatctccca tctgtttcta
     5101 ttatacataa tcaactttta aatagctacc tttttttcat aatttttt
//
LOCUS       DAYYFD010000139         5042 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000139 DAYYFD010000000
VERSION     DAYYFD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.143"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHA25_002743"
     CDS             complement(<1..182)
                     /locus_tag="ACHA25_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8763977.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHA25_002744"
     CDS             517..951
                     /locus_tag="ACHA25_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8763978.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHA25_002745"
     CDS             974..1294
                     /locus_tag="ACHA25_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763979.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHA25_002746"
     CDS             1435..1626
                     /locus_tag="ACHA25_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763980.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHA25_002747"
     CDS             complement(1697..1954)
                     /locus_tag="ACHA25_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8763981.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHA25_002748"
     CDS             2112..2375
                     /locus_tag="ACHA25_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763982.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHA25_002749"
     CDS             2399..2587
                     /locus_tag="ACHA25_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763983.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHA25_002750"
     CDS             complement(2670..2855)
                     /locus_tag="ACHA25_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763984.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHA25_002751"
     CDS             3070..3441
                     /locus_tag="ACHA25_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8763985.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHA25_002752"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHA25_002752"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHA25_002753"
     CDS             complement(3709..4137)
                     /locus_tag="ACHA25_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763986.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHA25_002754"
     CDS             complement(4197..4955)
                     /locus_tag="ACHA25_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8763987.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1757 a          784 c          986 g         1515 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 ga
//
LOCUS       DAYYFD010000140         4961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000140 DAYYFD010000000
VERSION     DAYYFD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.144"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACHA25_002755"
     CDS             complement(139..1338)
                     /locus_tag="ACHA25_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8763988.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACHA25_002756"
     CDS             complement(1468..2844)
                     /locus_tag="ACHA25_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8763989.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="ACHA25_002757"
     CDS             complement(2844..3500)
                     /locus_tag="ACHA25_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8763990.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..4787)
                     /locus_tag="ACHA25_002758"
     CDS             complement(3510..4787)
                     /locus_tag="ACHA25_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8763991.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4806..>4961)
                     /locus_tag="ACHA25_002759"
     CDS             complement(4806..>4961)
                     /locus_tag="ACHA25_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8763992.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1556 a          903 c          608 g         1894 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg
     3781 aatataaatt gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt
     3841 tcataaattc tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct
     3901 aacataatag aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa
     3961 gactcttcaa acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc
     4021 tctttcaatt tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt
     4081 ttatttgcaa tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct
     4141 acaaacacca tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta
     4201 ccagaaaaga tccctataat tttaaattta tgacttttta tttttccact tttttcaata
     4261 tctagtaatt caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct
     4321 tcatgaacaa taattgaatt cttatcattt tcttctatat ttttaccttc tttaattgta
     4381 aatactccac tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca
     4441 acattcttaa attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact
     4501 ttagcatctt ttagttttgc taatccatca tattgaatta ttttttcttc aatttctttt
     4561 aatttttcaa tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt
     4621 gatattgaag aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt
     4681 attgttaaac aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa
     4741 gttctatttt tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact
     4801 cctctctatt ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt
     4861 ccacatctga tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa
     4921 acttttttac attttccaca attaatacac ttatccttat c
//
LOCUS       DAYYFD010000141         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000141 DAYYFD010000000
VERSION     DAYYFD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.145"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHA25_002760"
     CDS             85..972
                     /locus_tag="ACHA25_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8763993.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHA25_002761"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHA25_002761"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8763994.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHA25_002762"
     CDS             2274..2720
                     /locus_tag="ACHA25_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8763995.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHA25_002763"
     CDS             2993..4345
                     /locus_tag="ACHA25_002763"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8763996.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHA25_002764"
     CDS             4516..4803
                     /locus_tag="ACHA25_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8763997.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1696 a          813 c         1029 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gaactgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYFD010000142         4930 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000142 DAYYFD010000000
VERSION     DAYYFD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.146"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..357
                     /locus_tag="ACHA25_002765"
     CDS             58..357
                     /locus_tag="ACHA25_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8763998.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            622..1497
                     /locus_tag="ACHA25_002766"
     CDS             622..1497
                     /locus_tag="ACHA25_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8763999.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1494..1712
                     /locus_tag="ACHA25_002767"
     CDS             1494..1712
                     /locus_tag="ACHA25_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764000.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1699..4326
                     /locus_tag="ACHA25_002768"
     CDS             1699..4326
                     /locus_tag="ACHA25_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8764001.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4341..4559
                     /locus_tag="ACHA25_002769"
     CDS             4341..4559
                     /locus_tag="ACHA25_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764002.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1930 a          612 c          988 g         1400 t
ORIGIN      
        1 ttgtacttat aaaggtagag gagttataga ataattaatt aaaaaggaga ggtttttgtg
       61 gaagcaataa aattaatgat ttcagaagaa caggaggaaa tgtttctaaa gcagttttat
      121 caaattgcta caaaagctat tgaaaaggct caagaaaatg cggggatgac aagggaatat
      181 ctcaatcaaa aagaaatggc ggaatactta aatgtgagtg aaaacttttt aaaaaatgct
      241 gtgagaaatg agggattgcc cgtagttgaa ttaggggcaa gaaaatttta ttcgaaaaag
      301 gcagttaatg agtttatgtt tgctcgaatg aaaagagggg aaaatattgg aagatgatga
      361 agtagaaatt ataaaaaaat gctttaaaaa aagaaaaaaa gtgttcccaa aagtttgcag
      421 actgggggaa cactcaataa taaagtgctg agaaaacttt atttatagct attatatcaa
      481 aaaagaaggt gaaaaggtgt tttcttctgg aaatgttgta gttgacaaac ttagagaaat
      541 aaattttgac ggaaatgtaa tccacacaca tggtacgatc aattaagaaa aaaaacgaaa
      601 aaaggtgttg agaagcccta tttgaatgca atagttatct tagcagaaat tacctattgg
      661 tatagaccta aaaaaatttt taacgatgat ggtcagctac ttggttatca aaaaaagttt
      721 cttgaagata ttttgcaaaa atcatataag caattaagca aaaaaacagg attatcagaa
      781 cgagctataa aagatgcaat tgtgtactta gagaaaaagg ggattatcaa aagggttttt
      841 agaaatattt attatgagaa aagagcagtt acattatcaa atgttatgta cattgtttta
      901 aatgtacata aattgataga aattactttt cctactttgg attgcgaaaa aaaggagtat
      961 atgccgaaaa ttcgtcagag gggtatcgaa aaaaagagat atgtctcttt aaaaaaaggt
     1021 agaggtatgt acaaatttgt atatacgtat acaaagacta cttcacagaa catatcaaag
     1081 actactctcg ttatggaaga agaggatatt aatataaaga ggatttacag taagattagt
     1141 gttctttatg acgcaaataa cgaagatggt cgaaaattat ttcaagtgta tcactatttg
     1201 agaaacgcta atgtagatac agaagatatc agaaaaatca ttgaatattt atctgaaaat
     1261 actcagtatc ttattgctga gtgtattata aaacaacatg aagcatgctt ttttcaaaga
     1321 aaaactgaag aagggttata cgattatgta acatattttt tgaatggact tcagaaaaga
     1381 tataaaagtc aatttttaga aaatgtagag cctatagatc agttcaaaaa agctttcgaa
     1441 atcaaagagt tacctgaaat tactatgcat aattggttga aaggagaaga atcatgatta
     1501 aaaatcgctt aatagatcaa atgtggtgtc ttgatcaaaa tacagatact ctcgaattaa
     1561 tcgagagtat ctgcttcaac acagtagttc tcgatttaag agctgaaaat gacaattgtg
     1621 taactcatat cataattaat caaaaaatgg ctcagcagct ctctgagagc ctaagaaaat
     1681 gggctgaagg agaaaactat gaaagcaatt aaagaaaaaa tcgttagtag aaaaataaac
     1741 tggttagcac tcagtgtact tgttttaatg attagcgttg tttttacaag taatattgtt
     1801 gaagctagca ttttcaattg gtttgatgac acaaaaagtc aaacagagtt tttgaataat
     1861 tctctttata gaccttacct aaaaaaatac aatggagatt tccttagtca gttcgggatt
     1921 tggcttggat gggctgtggt aaaaggcatg tttactgtga ctgattcgat tcaaaatatg
     1981 attccagatg tgttggattt gttcaatttc attgaaagta cagggctaaa taatgtttat
     2041 caatcagtca taaatacaat tgtcgttgga ttaatgatcc tttcattgat gtttgttggc
     2101 tacaagatga ttactggaaa aggaacaata gatttaagat ctgttggaat gaatatagtg
     2161 atgtctgtag cgttaattct tttaatgcca acaatgattt ctagcggtat tcaatttgcc
     2221 aaaatatttt acaatgatgc tactacgatt acaaattcag atgacggagt cgcttggtca
     2281 ttgataaaac aaggagtaac agatctggca tatattaata aaacagatca atactcaagc
     2341 attgataaaa cggaggatcg aaataagtta acacgaaaaa attttcaaca gacagattta
     2401 acacaagtgt tgacggataa agtgattgat aaattggaaa aagaaaatcc agctgcggat
     2461 aatcttcgct atgaattagt tgaaaattct aataatgaat ttgttgccac aaagttttct
     2521 gataacttct tatcaacatt ttctgatagc ttgaaatcag gttattatcg atatcaagca
     2581 aatctttggg ggatttcgat tgggttgaca gcgttggcaa tcgcctatgt gttcagcgcg
     2641 tttgtgatca ttacagcaat attagaactt gcatttaaac gtgtgttagg tgttcttgtt
     2701 tttgcgacag atattgaaac aggacaacgt tcaaaagttg ttttatccga tattttacaa
     2761 tgttacttaa cagttggatt tcaagggttt ggactatcta tgtttgctat gtttatcaat
     2821 tttttaaatg ctggacaagg tatctccaca aatattttta taaaaacaat tgcttatatc
     2881 tgtgctgtgt ttgtgctgat aaaaggcagt ggaacagtca tgagatactt tggtgttgat
     2941 attggactca aagaaggcta tgggcaactt gcttctgctt ttggtatggg cgcaatgctg
     3001 tttagaaaag gatcaactgg atttaatcgt gctaaaggaa gcggaaatgg aaatggatca
     3061 gacagtggag aaggagaaga tcgaaaacca gaaaagaatt tcggcgaaac tctatcaaaa
     3121 aaagctggca aaactggaag agctttggga tatgcccatg aacgtggatt atcaggttta
     3181 gcttcagatg gagctacaat ggctagtgaa agagcaataa aaccatttaa gagtatgcgt
     3241 gatatggcta atgataccaa gaataagttc aaagaaggtt tagatgatgg tacagtgtcc
     3301 gctattaata aaaatagcaa acctatgtta gctaaaaata aagaagatga aactggaaaa
     3361 tatgctgatt ccatgccaac acgtctttcg gatagaaaag atggtgctaa ggttgaaaat
     3421 gccgatagaa ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa
     3481 tcgaataata atcctgtaag tgcaattcag caaaaagttc aacaagatat tgaagaacgt
     3541 aaaaatgcta tgcacggtca agctaagtct gctgaagaat taattaatca gaagcgacaa
     3601 gaagctaaat acacgcctga agcaatgaat cgtgaggaaa tgttaaggaa acgtgtagaa
     3661 gggcgtacag gagctaatac ggacgaaaaa gaagcgttga ccaaagaaag aatccaagaa
     3721 gctaaaaact caaataccgg attagaaaaa ttggtcaaag aaaatctaca aaattctaca
     3781 agtagtcaag gtggccgatc agtagatgtc cgagaaaata ttcaaggttc attgaatggt
     3841 catgtcggac ggaatgttga tgtaagagag aatttacaaa gctcttcaaa tggtcaaggt
     3901 actaagacaa ttgatgttcg agaaaatgta caaaattcta caagtagtca agggtttaaa
     3961 tcaatggatg taagagagaa tgtacaaaat tctacaagta gtcaagggtt taaatcaatg
     4021 gatgtaagag agaatgtaca aagttctaca agtagtcaag gattcaaaac agtagatgtt
     4081 cgggagaacg ttcaaaaaga tagtggagaa atgaaagaaa aaacacaaaa aatcaatatc
     4141 gttgaagaca gaaagtcagc gaaaaaattt gattcaaatc atgaaacagt gattattgat
     4201 agtgaaatcc gtgaaaatga taagggatca aaaccaagac gaagatttac ctatgaagac
     4261 aacgagttat tcagagatac acttaatgat cctaatccgc ttttcgataa tctattaaaa
     4321 aaataaaagg gggaattgtc atgaaattaa ttctaaagat tagtacgcat gatttcttag
     4381 aacttgacga attatgtgaa tcagtaaaga acgcaacaaa tgaaaaaatc acggtcacta
     4441 attatattga actgatgatt caagaagaat taaagatgaa acatgagata aaaaacaact
     4501 tgaaatattg ttatctagaa aaaagtgaag agataatcca tgaattaaat ggcttttaaa
     4561 aattgtattt gacatgaatt tacgacaagg aattagtggg gattaagcga ttattctctt
     4621 ccactttaga gaaataatgg gttaaaaagc cagaatacgt agagcaatat tgacaaccta
     4681 taaaggagct attgttgaaa attgaagtaa caaaaagata aagggaggtt tacatctatc
     4741 agattaaaag tgatagtaca cccatgaaac ctgcaaaaga gttgaaccta atgtatatcg
     4801 gtttaactct ttttatttgc aaattaaaaa aatatcaccg aagtgacgaa tgaaactatg
     4861 accaaattaa gcaagaaaat atatcttgca aataaagtag tataaaaaga tattgataaa
     4921 gccaatcaaa
//
LOCUS       DAYYFD010000143         4894 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000143 DAYYFD010000000
VERSION     DAYYFD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.147"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..1730)
                     /locus_tag="ACHA25_002770"
     CDS             complement(120..1730)
                     /locus_tag="ACHA25_002770"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8764003.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1952..2197)
                     /locus_tag="ACHA25_002771"
     CDS             complement(1952..2197)
                     /locus_tag="ACHA25_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764004.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2923..4263)
                     /gene="glnA"
                     /locus_tag="ACHA25_002772"
     CDS             complement(2923..4263)
                     /gene="glnA"
                     /locus_tag="ACHA25_002772"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8764005.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4291..4683)
                     /locus_tag="ACHA25_002773"
     CDS             complement(4291..4683)
                     /locus_tag="ACHA25_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8764006.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1439 a         1053 c          799 g         1603 t
ORIGIN      
        1 gtcttttgct ttctatcttt tttcctacaa ataataacaa atcagaaaaa gagaaaagtg
       61 acaagaaaat tgcttatccc cttctcttgc tacttttctc tttctatcaa gttcaaaaat
      121 tattttgttt cgtgatctgc taatgatgct ccgacaaatg ctttgattaa tttttgcggg
      181 cgatttggac gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa ttttttctct
      241 ggcagctcta cgatttctac taaacggtta tctggtgaga cacctgaaaa gactagccct
      301 tgttcttcaa acaattgacg atatttgtta ttgaactcat agcggtgacg gtgacgttct
      361 tgcacaacat cggctccatc atatgcttca gccgtcttcg ttcctttttt gattttacat
      421 gggtataatc caaggcgtaa agttccaccc aaattctcga tgttttcctg atcagccatt
      481 aaatcaataa tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc atcttctaat
      541 ccaacgacgt tacgtgcaaa ttcgacacaa gccatctgca taccaagaca aatacctaaa
      601 aacggaacgt cattttcgcg agcataacga atcgcttcga ttttcccttc tactccgcga
      661 tcaccaaatc caccaggaac caagattcca tctgcgtctt tcaaacgttc ttctacattt
      721 tctctcgtta attcttgtgc tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa
      781 ccagaatgtt tcaatgcttc aactactgag atatatgcat ctggtaactc gacatattta
      841 ccgacaagcg caatccgtac ttttttcttc aagttcaaga cacgttcttc taattctttc
      901 cattctgtca tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac aatttgatcc
      961 attccttgtg cttgtaaaac taatgggatg gagtaaagtg tatctacatc tggcgactcg
     1021 ataactgctt ctggttcgac atcacaaaat tgtgccaatt tgtttttcac gctttgagaa
     1081 acaggttgct ctgtacgtac caccaaaata ttcggttgga tacctaaacc acgtagctct
     1141 tttacactgt gctgcgttgg tttagttttc atttctccag cagctttcaa gtaaggaatc
     1201 aatgttgtat ggatatacat aacattatct gtaccgacat ctgctttcat ttgacgaagt
     1261 gcttcaagga atggcaaaga ttctatatcc cctactgttc cccctacttc tgtaatgatc
     1321 acatctgaat cagtcatttt agctgctcgc atgatttttt ctttgatctc atttgtaata
     1381 tgaggaataa cttgaacagt tgctcccaag tattcgccct tacgttcttt acgcaaaact
     1441 tcagaataga tttttcctgt cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa
     1501 cgttcgtagt ggcccagatc caaatctgtt tcagcaccat catctgttac gaatacttct
     1561 ccgtgctgat atgggctcat cgtccccgga tccacgttga tgtatggatc aaatttttgg
     1621 atagttactt ttaagccgcg gtttttcaat aagcgtccta aagaagctgc aacaatccct
     1681 tttccgattg atgaaacgac accgccggtc acaaaaatat attttgtcat aagttcaaaa
     1741 actccttttt aatttatgtg caggaaaagt ctcagacaat aaacaaagct ccctattctt
     1801 caataagaat agggagccga aatttctgta aacttctgac cctctaaaag ggtgcccaag
     1861 gtgtataata caagctgact aagggaaagt caagtactca aatgaatact atccgcttac
     1921 aattttttct ttttcgatat cctttgagaa attatttctt aagtccttta tgagtgcaaa
     1981 aaaaatttaa cccacataat aagcttgata taaacatagc taacgttaaa cctaagcaaa
     2041 tcagaaaatc gttactgaaa aattttaagt gaaataatag ataaaaaaat aaaacaaaca
     2101 ttatgataga taatatccca cctaacaaag aactactttt tcctacttgc agcaacagtt
     2161 tatatagctt atcgtagaaa caatcttttt ctttcaccct tatttacctc ctttacgtaa
     2221 aaaacgtagt ccacagttct cggatttttg gcatatcttt tctattaaat aatatcaaaa
     2281 acaaaacaat tccactaatt ttttcaaaga tgaccttttt ttcaacaaaa gtgccttgta
     2341 gggattcatt gaaggtaacg aatcacataa gtaaaaaata aggagatgcg acaaaagtcg
     2401 tgcctcctta tttctaaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     2461 tatccatcaa acatgagaag ctgtttttga aattatttct tgtgattcgg agttaaatgg
     2521 taggctcaca acctctggat aaacggtatt caaaagccag tttctcggta ttaagccaaa
     2581 tttatgcaaa aatgtagaaa gccattttca caaaattttt cttaatactc gaaactaaac
     2641 gtctttttca aaacctcttg gtaaacggtg ttcaaaagct tgcatctgcg gtaataagtt
     2701 cagccaattc aaaaacaaga aaagctgttt ttgcttggct attcttatta cttgttgcag
     2761 ggcagctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
     2821 ctcaaaaaac ggcaaaatat gaggaactat ttttccgtta gtttctctaa tactcaggtc
     2881 aaaacgctga tttcacagcc tctaattcac ggtattcctg ttttaataaa gttctagata
     2941 ttgttctctt tcccactcgg aaacagtttg tctgaatgcc gcccattcga gacgttttgc
     3001 ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca atcattacat tatccttacg
     3061 caattctttg atagcattat ggatagttga aggtaagtcc tggatatgtg cagcttctct
     3121 ttcttcctca tccattacat aaatgttacg gtcaacagct gctggcggtg tgatctgatt
     3181 acggataccg tctagtcctg cttgcaacaa tactgccatt gccaagtatg ggtttgcaga
     3241 tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg cgagattctg gtacacgtac
     3301 taatggcgaa cgattacggc cactccatgc gacataaaca ggcgcttcat aacctggaac
     3361 gagacgttta taagaattga ctgttggatt gcaaacggct gtgtaggcac gagcatgttt
     3421 cagcaaacca cctaagaaat gataagctgt ttcacttagt tccaattcac cgtctttatc
     3481 atagaaaaca tttccttcat cattgaacaa agacatattg caatgcatac cagaaccatt
     3541 gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt ccatgcttgc gtgcaatcgt
     3601 tttgacaacg agcttgaagg tttggatatt atcacaagca tctaccacat ctgcatattt
     3661 aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt gaagcttcca cttcaaagcc
     3721 tagactttct aattccaaca cgatgtcacg gcggcaattt tcacctaaat ctgttggtgc
     3781 aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg atcccaccgt cttcgtctaa
     3841 tttaaataag aagaattctg gttcaggtcc taaattgaaa gaggtaaaac caaggtcgcg
     3901 catatctttc aatgctcgtt tcaaattccc gcgaggatcg ccggcaaatg gtgtcccatc
     3961 cggattatag atatcacaaa ttaaacgagc aacttttcca tgagcacttt cccacgggaa
     4021 gatcatccaa gtggagagat ctggatacaa atacatatcg ctttcttcaa ttcgaacaaa
     4081 tccttcaatc gaagaaccgt caaacatcat tttattttct aatactttgt cgatctggct
     4141 cactggaact tctacatttt tgatcgtacc taagatgtct gtgaacatca atcgtaaaaa
     4201 tcgtacgttt tcttcctcaa tgattcgttt gatttcttca cctgttatct gtttctttac
     4261 catggtatcc ctcttccttt tttaatttat tcagtcaaag aattgtcttt gcaatccttg
     4321 cgactgataa tgacctgttt tgcttaaccc accttgcgcc aaaatctcat cacgaaaaat
     4381 ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt gcttcttgtt ttttcgcctg
     4441 tgccatttcg tatacacgtt tgatcccagc catattcaat ccctctgaaa gatagtcttt
     4501 aatctcaagc aacatatcaa tatcattcag cgaatacata cgtcggtttc cttcgcttcg
     4561 ttccggatga actaaatctt gttcttcata atagcgaatt tgccgagcag acaaatcagt
     4621 cagcttcata actgtaccaa ttggaaatac ggacatagat cggcgcaatt ccttttctcc
     4681 catcagacgt cccctttcgt tttataagtt ggctaaagtg taccaattaa aaaaaatatt
     4741 gtcaactgca tatgttaggt tttctaacat gagatctcta ttttttataa aataacaaaa
     4801 aaataagaac acaaaaaagg tatctagttg attttcgtac cagatacctt ttatttttta
     4861 ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DAYYFD010000144         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000144 DAYYFD010000000
VERSION     DAYYFD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.148"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHA25_002774"
     CDS             186..2855
                     /locus_tag="ACHA25_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8764007.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHA25_002775"
     CDS             complement(2946..3560)
                     /locus_tag="ACHA25_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8764008.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHA25_002776"
     CDS             3680..4219
                     /locus_tag="ACHA25_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764009.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHA25_002777"
     CDS             4216..4554
                     /locus_tag="ACHA25_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8764010.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAYYFD010000145         4473 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000145 DAYYFD010000000
VERSION     DAYYFD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.149"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHA25_002778"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHA25_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8764011.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHA25_002779"
     CDS             complement(2020..2922)
                     /locus_tag="ACHA25_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8764012.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHA25_002780"
     CDS             complement(2919..3611)
                     /locus_tag="ACHA25_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8764013.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHA25_002781"
     CDS             complement(3654..4295)
                     /locus_tag="ACHA25_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8764014.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1381 a          956 c          770 g         1366 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tct
//
LOCUS       DAYYFD010000146         4433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000146 DAYYFD010000000
VERSION     DAYYFD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.150"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHA25_002782"
     CDS             complement(543..2048)
                     /locus_tag="ACHA25_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8764015.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIPNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHA25_002783"
     CDS             complement(2045..2731)
                     /locus_tag="ACHA25_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8764016.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHA25_002784"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHA25_002784"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8764017.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          918 c          731 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttggaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAYYFD010000147         4369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000147 DAYYFD010000000
VERSION     DAYYFD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.152"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..874
                     /locus_tag="ACHA25_002785"
     CDS             173..874
                     /locus_tag="ACHA25_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8764018.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1446..2462)
                     /gene="tsaD"
                     /locus_tag="ACHA25_002786"
     CDS             complement(1446..2462)
                     /gene="tsaD"
                     /locus_tag="ACHA25_002786"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8764019.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2459..2923)
                     /gene="rimI"
                     /locus_tag="ACHA25_002787"
     CDS             complement(2459..2923)
                     /gene="rimI"
                     /locus_tag="ACHA25_002787"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8764020.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2930..3472)
                     /gene="rimI"
                     /locus_tag="ACHA25_002788"
     CDS             complement(2930..3472)
                     /gene="rimI"
                     /locus_tag="ACHA25_002788"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8764021.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3456..4178)
                     /gene="tsaB"
                     /locus_tag="ACHA25_002789"
     CDS             complement(3456..4178)
                     /gene="tsaB"
                     /locus_tag="ACHA25_002789"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8764022.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1300 a          926 c          744 g         1399 t
ORIGIN      
        1 aatcattcgc ttcaaaacaa attattcaat accattgtat attttttaca taataacaaa
       61 ttatatttgt aaagtattaa aacttgtttc taatgttggt atgttaaaac tttacaaatt
      121 ctttaaaaaa agtgacaata aatatagtga acaataagga gaggactttt taatgcgtgt
      181 taataaaatc gattttcact tttcaaaact cagatatcaa cctgattttg aacatttctc
      241 ggatgaagag ttccaacaga tcaaggaaca tactgttctt cgttcttata aaaaaggaca
      301 gatccttttc gacgatggtg atcccagaca acggttgtat atcttgacca aaggactcgt
      361 tcgtttagaa cgttatgatg aaagtgcaac ttattactac tatgactatg tgaatacaaa
      421 taaccttttt cctatgtcag gaatgtttgg agaagagacc tacacttata cagctcaagc
      481 gatgactgat attgaaacat tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa
      541 aaatcccgaa cagttgatct atatgataca aaaaatttct aaagtagttg aaatacatga
      601 attgcgtatc caaatcggat tgacctctag cgcctttgac cgggtaaaac aaaatttatt
      661 cattttgaaa gaagaattgg gcgtaccaac aaaagatggt catatcacca ttccttatcc
      721 gatcacctta aaagaattag cagtgaatag cggcacgaca cgtgaaactg ctggacaagt
      781 aatcaaacaa ctacgtgatt ccggactgtt agattatgac aaaaagcgtt ttactttctg
      841 taagacagct actactccag ctatgagaga ataacgcgaa caaaaataag cagtgaccct
      901 gatatttatc agggtcactg cttattttcc tccctaaaac ttctgaaaga tccggcagtt
      961 ttagatcact gggcaagact caaagatttt ctatgtgtgt ttcagttatg taatattcac
     1021 tgtttgcaaa aaatactgat agattaacta gaggaagcgt cttgtcctca tccgacctag
     1081 attattcgat attcctcgac aataaacgat taatcagtga gggcaatcag aggaacggat
     1141 aatgatgaat aagctgtaaa aaatagggct ttgacaaaag cgtgtcacag cccttatttc
     1201 gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga
     1261 ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc
     1321 ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac
     1381 atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcaga tttttcacaa
     1441 cctctttatt cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt
     1501 cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt
     1561 cgaagagggg gaatcactag ttctacctca gggagtttag ttgccaaagc atcctgcaat
     1621 ccttcacgca acccttgatt ggccgctaca cctccagcta caatcagttg cttgactgga
     1681 taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag
     1741 ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa
     1801 ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc
     1861 gctcgtggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg
     1921 cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca
     1981 tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta
     2041 tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct
     2101 gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca
     2161 aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg
     2221 acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag
     2281 cataaggtga tttgttctac gtgatggcgg ctagctactt caggaactac gccaccaaaa
     2341 cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta
     2401 acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc
     2461 atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc
     2521 gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg
     2581 ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg
     2641 taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag
     2701 caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct
     2761 ggttatcttc aactaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact
     2821 gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt
     2881 ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt
     2941 ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga
     3001 tcgttgttgc ttgaaaacct aacttccgat aaagtctttg tgcatcttga ttactcatac
     3061 gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa
     3121 taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat
     3181 ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga
     3241 tacctagata tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc
     3301 atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg
     3361 tagtcaattc tcttagtacg taacgacgat cgatttgttc gattgttttt tctgtataaa
     3421 tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg
     3481 tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctacccgttt taaatagcga
     3541 ggaagaaaag cttgtttctc tgcaactggt tcagctaaag cacctaattg agccacagtt
     3601 acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc
     3661 tttatttcat agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc
     3721 ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacaatttct
     3781 aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac
     3841 atgggcacaa taattttatc cacaccgatg caattagccg cgatggtttt cagactagat
     3901 actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata
     3961 cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga
     4021 cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg
     4081 cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc
     4141 aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg
     4201 acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca
     4261 aaactagtac aatgatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac
     4321 acaaaaaagc tgcggtatca cttcatttcc ccacagcttt tccatcttt
//
LOCUS       DAYYFD010000148         4363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000148 DAYYFD010000000
VERSION     DAYYFD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.153"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..917
                     /locus_tag="ACHA25_002790"
     CDS             36..917
                     /locus_tag="ACHA25_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8764023.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            930..2540
                     /locus_tag="ACHA25_002791"
     CDS             930..2540
                     /locus_tag="ACHA25_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8764024.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2617..3276)
                     /locus_tag="ACHA25_002792"
     CDS             complement(2617..3276)
                     /locus_tag="ACHA25_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8764025.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3559..4224)
                     /locus_tag="ACHA25_002793"
     CDS             complement(3559..4224)
                     /locus_tag="ACHA25_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8764026.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1167 a          853 c          868 g         1475 t
ORIGIN      
        1 tagtatatat ttttagaaaa aggagtgggg tttttatggc agaaatcgtc aaagtcacac
       61 atttgaaaaa gagctttggt cgtttcgaag ctttaaaaga tgtctctttc accgtacatt
      121 caggtgaagt cgttggattt atcggaccaa atggttctgg aaaatccacg acgattcgga
      181 cacttttagg gattataaaa aaagacggcg gaacggcaac gatttttggg caagatgtat
      241 gggaagatag tttatctatc catgaacgtc tttcttatgt tccaggcgat atctctttgt
      301 ggggaaattt gactggagga gcgatcatcg atctcttcat gaagcttcat ggaaaaggga
      361 gtgctcaaaa aaaagatgaa ctgattcaac gtttctctct tgatcctaag aaaaaagcaa
      421 aaaactattc aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg gctgttgatt
      481 cggatctcta tttatttgat gagcctactt ctggtttaga tcctttgatg gaaacgattt
      541 tccaagaaga agttgaaaaa ttgaaagaac agggcaaatc catcctactt tcttctcata
      601 ttctcagtga agtcgagcga ctagcggata aagtcgtgat catacgcgaa ggaacactcg
      661 atgaagaagg aacactcgat gaattgcgtc atttgactcg ttcgaccatc actatagaaa
      721 cagaagcaga gaatagtcac ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag
      781 gtcacctcgc tcaatttgct gctgataacg aagctttcga tgtgatttta gctgaagcgt
      841 ccaaattagg tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat ttattcatga
      901 gacattatga aggatgagga ggaagcgaaa tgactgataa attttcacgc tggtccaccc
      961 ttttttctca aaaccttagc cgtgactgga aaaagatcct gatatggcta gtcggtgtca
     1021 ctgctttttc tggtggtttt gtccctgcgt ttgaagaatt gacttccgga caaggattag
     1081 ctggattata cgaaaccttg caaaacccgg caatgattgc aatggtcgga ccaactccag
     1141 cttcgaatgc tgctgagtat actttaggag cgatgtatgc aaatgaaatg ttgctttttt
     1201 gcagtttgtt tgctgctgtg atctctgttt tacacgtggt gagccaaacg agaaaaaaag
     1261 aagaacttgg tctgattgaa cttattcgtt cctttcgagt cggacgtcaa gcaaactctt
     1321 tagctgtcat cgctgaaagt attctgatca atttgctttt agcaatatcg atcagctgta
     1381 cgatgatttt tttccaagcg gattcgatca ctgcagaagg tgcttggctt tttgggctat
     1441 ctgtaggtag tatggggatt ttggggagtg ccgttgcact gtttgctgca caactgatgc
     1501 caagttcagc cagttcgaat ggtctgtctt tagcgttttt aggtggactt taccttctac
     1561 gagcaataac agatgtagct gattcctccc tttcgatgtg gaatccttta ggctggtctt
     1621 atttgaccta tccatttaca actaacaact ggattccttt gctttatttg tttttgttca
     1681 gtctaatctt gataatcagt tcatttgttt tagaggaaca tcgagatttg aacaccggct
     1741 atatccccga acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt
     1801 tattattcta tctaaataaa gggatgacaa tcagttggct tatcactttt ggtttgatgg
     1861 gggcggcata cggctctatc tacgggaata tgcaacagtt tttagaaagt aacgacttaa
     1921 tgaaacagat gtttatccaa tctggcgtct caattgaaaa aaactttaca agcacgatca
     1981 tggtagtctt gatttgtcta gctgctattt taccaattgc tagcatcaat cgtttattta
     2041 cagaagaaac ccacggacgt atgaatcagc tgttcgcaac aaaaatttct aggaaccaac
     2101 tttattggac caatatgtta ctagctattc tatttgctat attggcaatc ctgattagtt
     2161 ctggtgcact agggctttct gctttggctt cgatgaagga tgcgaaagac atggatctat
     2221 tgatgtttct taaagccggg ttcaatttcc tgcctgccgt tttagtgttt atcggaatta
     2281 gtggctttct attcggatgg cttcctcgaa tcggtaaact tgcttatatc tacttagctt
     2341 actcctttgc actcaattac tttggcggaa ttttggattt accagaatgg ttttccaaaa
     2401 cagctgtccc tagctgggtt ccacggatgc caaacgattc ttttgatctt tccgtgttcc
     2461 ttacgttatc atgtatcagc cttgcactgt tcgtccttgg ttctattggt tataggcgaa
     2521 gagacttact agaaggataa taaaaaagca tgtacaattt catgctagcc tccaaatctg
     2581 ggtcaaaaat ccaacatttg gaggtcattt ttttatttac aagtcttcta actgctcttt
     2641 atcaatgatc cagacttctc gattctttct ttttacataa ccattctcct ctaataattt
     2701 aaattttcta gataaagttt cgggggtcgt accgataaat gcagcaagtt ctttcatttt
     2761 cattggcagg aaaaagtgat ccttccctgt cgctttagct aagtcaagca gatagagagc
     2821 gactcgttct tctacttttt ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa
     2881 tttctcaacg ttgattgcta aaagtttcag actaagggcg ggataatgca gtaagagttc
     2941 cgagaaatct ttttgtctca atatgcaaat ttccgttttt tctagtgctt cgccaaataa
     3001 agactcattt ggaacgccta gcaactggct ctctccctca tatccgcccg gctctattac
     3061 tcgtaaaagc tgttcctttc cgctagcaga taattgatac actttcatac tgccggaggc
     3121 cacgatcact aattgaggtt ctccattcgg cgcaagaatc tgttctcctt tttccacata
     3181 gtgatgtttg acaagtgcgt gtactttttc ttgatcttgc tgtactaagt gattgaacaa
     3241 aggaacaaat gaaacacata aatgatcatg actcataatc tctcctcctt ctaaacagct
     3301 atttctaata aagaaacata acttccctta ttccaactga taattttctt ttatcatagc
     3361 ataaacgtca agcgaaccag ataccagcaa atagagtgga tttctttttg tcccttgctt
     3421 tattttcctt cttttgagac cttaagtaat tgattcctat caatttttta aatatttact
     3481 ttttgaataa tcaaaaacaa accttcactc gcaagccgag agtaaggttt gtttttcgca
     3541 ttgcttattt cgtgttgtct attgaaattt ttccgcacct agcttttgat atagtggtgc
     3601 tagttcttgt aaggtttcag cggttatttg ctcaacgtgt tcgatttctt gcggatagaa
     3661 gaatagttta cccatttgaa aggttcggtc tctcatcgct tttttcaact cttcttgttt
     3721 ctttgtatcg gacaaatagt cagacaaagc taagtggtcg tcaagttcat gttctggttg
     3781 cggccatatg taataatcct tgatatcgac tgttttgctc cactctttca gtatcgcaag
     3841 caactggtta tacgctgcaa tgctaccata tcgttcttcg cttttataat gttggtacat
     3901 caaatagatt tggtattgtt tttgattcaa caaaactgct agacaggcat tctcgtcttg
     3961 acgagattcg cttcggtacg cgctccaaaa atgactgcgt aaattccagc cattcgtcca
     4021 gctttcgatt ttaggtttgc tcatgccgta catatcaggc aaaagtgcag ctgtctggag
     4081 ctgaatctct ttccattttt gccagtgttc tttatattct ttcttgattt gttcaatctt
     4141 atctgttgga tggctctctt tgagctgttt gaatgcgtag attggtcgat caaacaactg
     4201 ataatctgat tttccaagtt ccattgtttc gcctctttcc gttatctttt gtattctacc
     4261 tgaattttgc gatatggaca aatagaggtt gtgaaaaagc tgttctgcat caagtcataa
     4321 gaacagccaa acaaaaaact gcgatgagca tcaccgcagt ttt
//
LOCUS       DAYYFD010000149         4335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000149 DAYYFD010000000
VERSION     DAYYFD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.154"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..498)
                     /locus_tag="ACHA25_002794"
     CDS             complement(103..498)
                     /locus_tag="ACHA25_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764027.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            complement(578..2929)
                     /locus_tag="ACHA25_002795"
     CDS             complement(578..2929)
                     /locus_tag="ACHA25_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8764028.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            complement(3054..3743)
                     /locus_tag="ACHA25_002796"
     CDS             complement(3054..3743)
                     /locus_tag="ACHA25_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764029.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            complement(3757..4209)
                     /locus_tag="ACHA25_002797"
     CDS             complement(3757..4209)
                     /locus_tag="ACHA25_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764030.1"
                     /translation="MKKTILSFFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
BASE COUNT         1395 a          867 c          548 g         1525 t
ORIGIN      
        1 aataaactag ttatccagtt tttcttttat gtcttccatg gtatatttta tttttcacat
       61 cagaatttga cttctttaac ttttacaatt tttttaacac tattattcta ttacaacact
      121 atatatttct aatttagggt taaaaataaa tctttttgat acttttgcct ttacaatata
      181 attgtattct ttcttttttt ctttgaagtc gcttgattca actgtaactt caaaccacat
      241 ttcatttgga gactctttta atttttttac ttgaaatgaa ttatctcttg gatcataagg
      301 tggtactctt tcaaactcag gaagttttct ctgtatttgt tcttcaggag tattcgcaaa
      361 gtaatctact atcttgacag ttattaaaag tactactatt cccaaaacta taacaaacat
      421 aaagataatt ccacatccag aattattttt attattttga gtcagattat tccattctat
      481 ttcttccata atcatcatgt ccttttacaa ttcatatact tattttatcg tatatattta
      541 aaaaggaaaa ggacactgtt taaatagtaa acgattctta attaagttta cttctatttt
      601 cttgaaattg aataattttt tcagaaaatc catttgcaac tttcccctca tctccaataa
      661 aatcaagata agtaaaatct gtcccaagtg aattattaac tattttatca atgaaaaatg
      721 aaattatccc tacacctaag atatcaactg tttcgtcatt ttctaataaa ctatttgtta
      781 ctgaagcgat tttatctgaa gaaccaaatc tgttcttaac aaattgatta aatctatcaa
      841 tagcaccctt atcacaatag tacgttttta aagccgataa agctgttgaa tcagaatttc
      901 tttttgctaa ggtataaaca tttaccgcgt caacatcttg gtataaatct tctagactaa
      961 atgttccacc tgcagccgta cctactaatg caaatacacc ttgctccatt tgtattccta
     1021 atttcttaac ttcaccccag caagtcatta aatctcctaa ccaacctgca aagtcagaaa
     1081 cactatcatt gattgtgtcg gctagaaaca tctgtgattg taacgtcaca attaagtgag
     1141 gcaaacctat tattgaacca gtattaggat ctataaaata attatacagg ccctcgctat
     1201 tagttaattt attcctatat tcatcataaa tttttctatc atgctctgtt acaggagaag
     1261 aaatgatgtc ccatgacaaa ccagaataat ttacatatct ataaaatgtc aaatgagggt
     1321 tccaaggatt atcaagacca gcttgtgata gatattgata cgcacgtacc atctgattta
     1381 taaccttctg attgtatttg gcaatgtaat tatcattatt agtatcattt gataactcat
     1441 taaatccgct atctctaccc gatgtagcga ttaaatcgat accaacagct ccgttgccac
     1501 ttcctatggt aaattcaaca aattgatcaa aagaccattg tctaggcata ggaaaaccta
     1561 aattcccact ataaccagtc gacatatctg aaacaaatgc aaacgaagca tacccgttat
     1621 tgattatttg agaacaaaca tttcttgttc cgtaaacacc cgtttgatac ccgctatatg
     1681 acattacagc agataccccc ttaaaatatt ctccagcata tgcaagaatt tcttccccta
     1741 acatgtctag atcaacagca aaataaataa ctgttgttaa tggtatattt agattttttg
     1801 ctgcatctat agccttctta ccatctgata agccacgatc ataagtaaaa taagctaatg
     1861 ctgcacctcc atcttgatag ataggaaaca cagataaatt tttagaaaaa atattattca
     1921 attcctctgt agttagatac ttatttcgtt catcttttcc aattccaaca gtaccagtaa
     1981 gatatctacc aactattttt atatcattat caaccaatgt ttggatttga gatctagtta
     2041 attgcgtaga agtatctgcg ccatctgcag atcttctaat atctccagcg ctagacaata
     2101 gtccttttat tacaacaaca tctgctattt cagtatattc aggcaatacc ataaattttc
     2161 ggaatgccag tacttctaaa cccatatatt tagtgaaaat accatcgaat gtaccgtctt
     2221 caaaataacc attaacatat aatccatatt gaattattct tacatagttt cccgagttac
     2281 cttcagacac aggataagtt ttactagttg tcccattacc ataagaccct gttgcttcac
     2341 tgggagaata tcctagttca gcttgtaatg caaaaattaa tgcttgattt gaatttcttt
     2401 gataaatacc atcacaaggc attattcccg tgtattctaa ataatttaca tttaaccatt
     2461 gctgcatttt tctaatttta ctatctccgc cgggaacaag aacaaaagca ctcatatcaa
     2521 ataacgcttt ggcccatttc gcatccaaaa ctccagttac agcgcttcca ttaaaccctg
     2581 catcagtctg caactctttt acagcagtat ctaaatatgg agtatatacc ccatcgaagc
     2641 cacctggatt aatcccctta caccaaaagg caccttgaat aagaaataca atatttttag
     2701 gtagattttt gccccattta ggagtaacct gattatcgta cattttttca gtggtaggtc
     2761 caaaattagc tactagatta gtaataccaa gttctacttg tagtccttca ataagtccat
     2821 agattgtagg ccaccctgtt cgcccatttt ctggaacctt atcaaaccct gtaacattac
     2881 tgtatttctt gtttagccat ttctgagtac ttaaaaccat ctcatccata aaaatcccct
     2941 gctttcttgc cgaacatacg ttcgtttatg tgttgattat attttatatt ttagtgtttg
     3001 tcaacaacta aaaataataa aacagcacaa agaaatctct ttgtgctgtt ttattaaata
     3061 ttttgaacaa cataatacgt acctgcaaca ggattataag tgtaataaat acctatattc
     3121 attgtccaac caggcccgtt tggtgcaaag tatttgaatc cttcacgagc atacaatgcc
     3181 attccttgaa tagaaccaga tttagttgcg ttggcttgat ttacatttcc tactcttcca
     3241 tcgtcaatgt aggatttcca acgtaaaaag ttgcctgtac catcagctgg ttttacaccc
     3301 atacctccaa aacgccatcc cgcaccacta aatggctgtg atttatagct tcctcctcct
     3361 gctagtacaa gaaggtgtcc gctagtacca acatcgggtg gaaatgctcc tcgaggcata
     3421 attggagtag tttcaatatc atttttgaat ttttcagcaa caattttttt tgcttctgtc
     3481 actgaaactg aagcaggatc agtttctgaa ttatatgaac cttgaattat atctggatta
     3541 tccatagata caattttagc ttcccctttt aaaaaaccac cttctggcaa tgcaagtact
     3601 ttatcttctt cttcaaaatc agcaaagtat cgtgaggcgt actgctctgg atctgaaata
     3661 acttctttct cagcagcata tactgcattt ccaaatgtta acatcataag tagcccaaca
     3721 gaaacaccaa aaatcttttt cataatgaat cttcctttac tctaattcca ctgaattagt
     3781 ttcttttatc tgctttaatt catttctttt actatttaaa agctctttta gatccttaat
     3841 cgaaatagcg ttggaatcag tattaacatc aataaactgt ctgtgatttt gtttataact
     3901 aactaattcg gatttgccat ctcccattaa ttgcccacca gcagacaaga caacaatttt
     3961 atctgagtca ctaattcctg gaatactttc ttctaactgt tccccggata aatcaccaaa
     4021 ttctttatac aattcttcat ctgacataga atagatattt ttggtcaaac tcattttttt
     4081 attattatgt tcagctactc tttgatcttt tatttgagcg tttgaattaa cagaatctaa
     4141 attcaaaaga aaatagcccc caattaatat tgtaaatact gaaaaaaaac ttagtatcgt
     4201 ttttttcacg attctcacct cgctttctta ttaaatttat aatatatatc tacgataaga
     4261 taattggttc tgttgcaaag ttttaaatct actatcaaat aagatagaat aatagaaaaa
     4321 gatagcagga ggaat
//
LOCUS       DAYYFD010000150         4305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000150 DAYYFD010000000
VERSION     DAYYFD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.155"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..543)
                     /locus_tag="ACHA25_002798"
     CDS             complement(58..543)
                     /locus_tag="ACHA25_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8764031.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(545..1216)
                     /locus_tag="ACHA25_002799"
     CDS             complement(545..1216)
                     /locus_tag="ACHA25_002799"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764032.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1479..2672)
                     /locus_tag="ACHA25_002800"
     CDS             complement(1479..2672)
                     /locus_tag="ACHA25_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8764033.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2756..2956)
                     /locus_tag="ACHA25_002801"
     CDS             complement(2756..2956)
                     /locus_tag="ACHA25_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8764034.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3380..3613)
                     /locus_tag="ACHA25_002802"
     CDS             complement(3380..3613)
                     /locus_tag="ACHA25_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8764035.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3620..4051)
                     /locus_tag="ACHA25_002803"
     CDS             complement(3620..4051)
                     /locus_tag="ACHA25_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8764036.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1070 a         1069 c          837 g         1329 t
ORIGIN      
        1 cagggataag taagtaaaaa aataacatgt cattagatag tagacagttc caactcttta
       61 tttacctttt ttgtatgctg cactgtcaat ccaagttcct aaattgttgt atccatcatc
      121 acttatataa tcatattggc catcaataat ttcgtcaaac cacaaatatt ctccatctgg
      181 tttttgccca attattttaa ttactgattg actagtagtt gtcatctggt taaaatcttt
      241 tattttacct atattaacgg ccaaaatagc atttcctcgc ttataactct cctggatttc
      301 atatttaaca tattctcgat caagagtttc tttacctatc agcaccacag tcactgatgt
      361 tccaattaat tgattatcta tccagttttt tatagcaata tctccattac gtttaacttt
      421 ctcaaattca gccttatcta caaaaccagc ttcctttgtt cctcgcaaag tccagctgtt
      481 tctaactacc attgctcttg ttacatctgc ataatggaaa ctaaaaaaaa cccttctcac
      541 catattaatc ctcctgaata ctatttatta aactgacaat ttgttcaact tttttatcta
      601 cttctccttc agtatctaat ttttctagcg cttgaaattt actttttaag gaatcatcaa
      661 aataatcact gaaattctct tgaatcttat caaacaaaac cttacttgca cctttggtaa
      721 atccaatcgg aactactata ttttcatttt gaacagataa ttcaaattct tcaatcattc
      781 catctgctgt aactatttca cctgattcaa gtttgtttcc aaaaacaaaa atactaatcc
      841 cagactctgc aataatgttt tctcgatatc ttctccactc atgaggcata tcaaggggaa
      901 gtggtcttaa agaaagtacg tcttgtatgt tattttttct taaatttctt ctctgcttta
      961 gaactcctga aacaatacta ttaccaattc caagtccgta acctgatact attctataac
     1021 cggattcaga aagcctatat gctaatttct cgacaaattt taatcctttc gattcagatg
     1081 taaatggagt ataatcaaca gcacttcccg aaataaaaat agtttttctc aaatactttt
     1141 ttctaatact tttcaaaata tcagtaatct cagaataatc atctacttca acaacgttta
     1201 tcgaaaatct ggacaaatca ttgatttgta gtcttctttt tgtcagttca taattatatc
     1261 tctcttgatc aaaaaaatcc tcagaatcga caaaattttc ctttaaaatt atatgggatt
     1321 ttagattatc tttttagaag ttcttgaatg tcggtatagc ccgtgtttcc gggcttttcc
     1381 ggcatttttg atattgttag atacttacaa atgttcttat tacctctcac catttccccc
     1441 tcaaaagtag taaatggagt agtaaaaccg gccttgtgtc aggctgccag ccgttccatt
     1501 tctgcccttg cggaatcgaa tgtggcgtgt gcataatagt tcagcgtcat ggtgatattg
     1561 gaatgtccca taatgtactg caaggctttg gggttcattc ccgcattggc taaccgggtg
     1621 cagaatgtat gacgcatagc atggggcgtc gtcgttttcg gcaacgcttc ttcatggctc
     1681 ttgttgtact tcttcacaag cctgccaaac atcccgccat aatctaccgc cgtcatggga
     1741 tagccgttct gtttgagaaa caggaaattg gcatacccgt caataatgaa cggctttgca
     1801 cctttgcggt tttttatcac tcgctggaac gcctgataca cttcctcatt catggggatt
     1861 tttctgacgc cgctttgggt cttgggttcg tcaatatagt aaccgacgcc ggtatttctc
     1921 aaaagctggt ggctcacgat gattacccgg ttttcaaaat ccaaatcttt gtccgtcagt
     1981 ccgcacagtt ccgaaatgcg aagccctgtc cccagcagaa taacgattgc gtcataatgc
     2041 ttttggtaaa ccttatcacc ttgaataaag gacagaaaac tttcttcctg tgctggtgta
     2101 agaggcacct ttggttccgt atcgtcgtcc agaacgtcac taagctggaa gtcaaacggg
     2161 ttttttcgga tgcaatcgtc ctgtatggcg gtgtaaaaag ccgctttcag ggaacgcttg
     2221 tcgttattga tggtcttata agaaaggccc ttttctttca tccgaagcgc ccattccttt
     2281 gcgtcggaca gcttcacact gtcaatcggg cagctcccca gccgatcttg ttccagaata
     2341 cgcatcagcc gttcccggcc cagtttggtg ttgtgcttca cattgccccg gtgcctgatc
     2401 tgcttggcgt agagctggca gacggtcatt ttcttgccga tggtgtctat cccgtcgtca
     2461 aggtctttct ggatttcttt ctccttttcc cggagtgaaa tatcctcccg tttacctgcc
     2521 ggggtcttat ccgtaggcac taacttccat gagtaaacaa actgcggttt tccaaaggta
     2581 tcaacatatt tgtaagcata tcgtccgtct tttctctggc tctctccaga ccgcaaaacg
     2641 cgatttttgc tgtctcgtct tttttctgac atctcgtcat gctcctttcc atgacggaaa
     2701 gagccgcata tatgcttata aattattata acatatctgc ggcccttttt cactattaga
     2761 ttgtgtccaa agtgtcaatt attttctcaa actgcttgcg tttgatctgg atgcggttgc
     2821 cattcataat gacccagcca gaggccggat tttcctcggc cagcttacgc aatttgtttt
     2881 ctccgatacg gaaatattta gacgcttcct caatcgtcag cgtgtatttt tcccaaatag
     2941 gcacatcatg gttcataatg cagccccctt tcacaaattc aaaggtttgt gtgatttagg
     3001 ggcatagacg gacggctgct ggcacagctc cacgggactt tcacccccgc gtggttctca
     3061 cgcggcccta cccattgcct gcgacgcttt taacgctcgg actgtggcta taagggagta
     3121 tcatagtcct gcctgtacgt cgtcgcccgc aagccgccac gctcacattg cggcgtggtg
     3181 ttcctcgctc cgcttttccc gtagggaaaa gtatcatggc gcaccagccg tccggctcgg
     3241 cacagacaga atgtgtccgt atgccctatg atgcgccgcc gttatcttgc gggcatattt
     3301 atcaaggtgc gatcaggcca ccgggaaaac accgaaacgg ggaaagggta agccgtttca
     3361 tgctgccggg gccggttcgt cagttcgtga cgatagcgtg gatgagcttg atttccagac
     3421 ggcgcttcat ggcctcgtcc agacacacat gcggatagcc gttttcgtca tagagcgtcc
     3481 gggtacacag cttgttcatg taaccgctgt aatactgcaa aacgcgctct acggctccgg
     3541 catccccagc gcgggcggcc tcgatcaccg aaaggggcaa aagctcaatg cccttgtagt
     3601 tcgggttctt catccgcgtc taacctccct tcggcatcaa cgcctttagc tgtttccgca
     3661 gctccgataa ggtatttgtc ctgtgccgct gaacggcact ccgggacatt cccacaaggc
     3721 cgccgatctc accatctgcc atatccagaa cacagtgcag gattaaaata ctctgctcca
     3781 tgctgggcag cgcggcaaag gcttcggcaa caagctcatt gttgatatgt aggtcatagc
     3841 catgcgacga aaacgtaaag ctgtcgctgg ggtagtgatc tactgtacag agcttgtcca
     3901 aatctgcctg tgacaaagag cttagtgaca cttcacggtc acggcggcgc ttcatttcag
     3961 ccaagtagct tttggcctcg ttacgcagta ccgtcttgca aaaagcatcg aacatgcacc
     4021 gcgcttcata gtcgcggggg atagcatcca tcttctcacc ccctttctgt gggggactta
     4081 ggtggttctc tccctttccg ctaacaagac tttgacaagg ccgccagcta cccgctaaat
     4141 acctcctttt tgaaaatttc tcaaaaaatt tttttgggga cagccgggcc gcaaaagacg
     4201 acaaaaatcg cccggcaggt cgcaaaccta ccgggcgaga aaatgctttg ctatgctgct
     4261 gatttacatg gtatagtaca tactcataac cggaatagcc ctgtg
//
LOCUS       DAYYFD010000151         4188 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000151 DAYYFD010000000
VERSION     DAYYFD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.156"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..794
                     /locus_tag="ACHA25_002804"
     CDS             <1..794
                     /locus_tag="ACHA25_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8764037.1"
                     /translation="TGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFL
                     YENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITL
                     GMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVV
                     QFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSN
                     HTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            811..1245
                     /locus_tag="ACHA25_002805"
     CDS             811..1245
                     /locus_tag="ACHA25_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764038.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1862..>4188
                     /gene="esp"
                     /locus_tag="ACHA25_002806"
     CDS             1862..>4188
                     /gene="esp"
                     /locus_tag="ACHA25_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8764039.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1545 a          583 c          789 g         1271 t
ORIGIN      
        1 ttactggaaa aatagaagat ctattccatg aaccgttaat tgaatataca gaaaatctaa
       61 gtaaaactat cgagcaaatc aaaatagata atctgttatc acaaaattac gatccagaaa
      121 tatatctgtt tctttatgaa aataaaatac tggaatatgt taaaaatgga gatattcgaa
      181 atttagaaaa catggttttt aatctgagta atggaataat tccaagtgtt agtggagata
      241 cgattcggtc tgaaaaaaat tattctatca ttgtttttga aaagttagca caaacgtcta
      301 taacattagg aatggatata atagaagcgt atcaaagtag agatgctttg atacaagaaa
      361 acgaattagc tgtatcgtta cctgaggtct taaaagtgag ggattctggg attgtttatt
      421 atactaagga aatcggaaaa actaagatag agcatctttc tcccttaatt tcttctgtcg
      481 ttcaattcat cggcttaaat atctataaaa gaattactgt aaaggaaata gctaattatt
      541 tttctgtaag tgaaactaaa ttaagagaaa gctttaagaa tgaaatgcat attacgattt
      601 ataattatat ctctaagcga aaaatttcta cggctaaaat aatgttgaaa tcaaaccata
      661 ctatcagtga agtttcttta ggtttaggat tttcagattc ttcacacttt tcgagagtat
      721 tcaaaaagta cgcaggtgta tctcctaagc aatatcaatt aggtttagtg gacaatttca
      781 acaacattag ctgagaggtt ttattttatt atgagactat atcatttacg tagtttggag
      841 gagttgagga aattggattt taacaaaaaa tttttttggg aaatttctga tttgagttgt
      901 ttcgtagaat ctgagcgtct aagtaatggt aataatagtg tgttaaatga atatgacaaa
      961 gtagtaatta ataaagtaaa aagtttatgt gaagaagcta gagaatctat cagtcatatt
     1021 aatggtagca attttaaaca aacattattt aatctgatta agctagatgc taaaatatca
     1081 agttatttat tctttttgat acatgatgaa tttatggatt atcaaaaatt ggatcagtta
     1141 attgaaagag aaagctggga agattattac gtgggtctaa ctttttcaga aaagttaaac
     1201 aactacaagt taatagatta taagtatttg agtgaaagca attaattatt catgtagagt
     1261 actagacact ttaaatatgg acaattcaat tataaagaaa aagaatcaat aatttaatga
     1321 ggctgtgaca aatgtcattt tttgtttcgc taaaaacgag tgaaaaaatg ttgataactc
     1381 caatcattcg tttaagattc taggaatcat tcagaaatgc tgtgaaaccg atactttata
     1441 attataaaga aggtgatttc aaattccgaa gaaggaattt gaagtcacct ttttacttat
     1501 gtcacagtct cattcagtaa tgcgggataa ggctccttat tccgttattt gaatactctg
     1561 aagtttaatc taagaaaggg taatattttg aaaacatgct tcatcgcata gaaatgaact
     1621 taatttcaga aagaatacat acatatcttt ttatatgttt tcagctgctg aatggttaaa
     1681 aagcacaaat atccgaaaaa aacatgaaga aatgtacttt ttttgttgta atgttcatga
     1741 aattctctaa gtaaattagt taacataagg ttagtaagat tcctattctg tgaataatac
     1801 gtatcagatt tatcttttat tcttactagg aaagtaatta ttaagggagt tgatttgaat
     1861 aatggttagc aagaataata agagagtatt tctagaaaaa acaaagaaac gagtattgaa
     1921 gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt ttgattggtg ttggtcttgt
     1981 cttgggaaca actgaactag ttaaagcaca agatgaaatt tctccaagca gcccacttga
     2041 aacagcgata agctccgtac aagtaggtga caaagtagct tcggggaata cttttcagga
     2101 gaatcctggt tatacgaaaa attataactt ttcggattta caatttagtc ctcaagaact
     2161 aactggagac acactaaaag gcaatacaat tggatttgaa gtttacggaa aacataatat
     2221 tgcagcatca actaaaaact gggaaatccg tcttcaatta gatgaacgat tagctaaata
     2281 tgtagaaaaa attcaggtcg atccaaagaa aggaattgga tcttctagac ggacctttgt
     2341 aagaattaat gattcgctgg gtagacctac aaatatttgg aaggtcaatt atattcgtgc
     2401 aagtgatgga ttatttgctg gggcagaaac aaccgatacg caaaccgctc ctaatggggt
     2461 aattacgttt gaaaagagcc tagatgaaat ttttaaagag ataggcatag acaatctcaa
     2521 gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat caagacgatg ataaaattgt
     2581 accgggtatt gatagtacgg gctacttttt aacagattca gatgatttct ataatagctt
     2641 agatgtatct gaaaataatc ctgaccagtt taaacatgga tcagtaagtg caaaatatga
     2701 gaaacctaat actcaaacaa aagatggttc aggatccaca ggtgctaatg gagctataat
     2761 cttagatcat aagttaacga aaaattataa tttctcatat tcatcttccg caaagggcac
     2821 gccttggtat gctaattaca aaattgatga acgattagtt ccatatgtag cgggtataca
     2881 gatgcacatg gttcaggctg acaaagtaac atatgatgtt tcgtttgaat ccggaacaaa
     2941 agtagctgat ttagcaatcg agcgacggga aggtcatgaa aattatggca taggttcaat
     3001 aactaataat gatttaacta aacttatcga ttttgctaat gcaagtccac gtccagtcgt
     3061 tattcgatat gttttacaat tgactaagcc attagatgaa atcttagaag atatgaaagc
     3121 aacagcacaa gttgaagaaa ataaaccatt tggtgaagat ttcatctttg attcttggtt
     3181 gtcggatacg aataaaaaat taatccagaa cacttatgga acaggttatt attatttgca
     3241 agatattgat ggtgatggaa accctgacga taaagaagag agcggagaca cgaatccata
     3301 tatcgggaaa cctgaattag aagaagtata tgatgttgac acaacagtta aggggaaagt
     3361 attcatccac gagttagcgg gaacaggtca caaagcccaa cttgttgata aagaaggtac
     3421 tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat ggggctccaa tttcagatac
     3481 tgtagaattt gaatttacgg gtgtagattc aagtaaatta atcgcgaaag atgaattaaa
     3541 aatccaaatc gtttctccag gttttgataa accagaagaa ggttcaaccg ttattaagga
     3601 atcaccaaaa gcggttgata aacaaaccgt ggtagttgga tttaaaccag atgctaaaga
     3661 atcaattcgg aataataaaa acttacctga agatgcagag tattcatgga aaacagagcc
     3721 tgatacttct aacgttactg atagtacgaa aggtattgta actgttaaga tcggaaatcg
     3781 aactttcgac gtggatgtag agtttgctgt aaaagcttct caagctatgg aaaatgatgc
     3841 aacatacgta cctataacaa caactccaga aacgacagtt caaagtggaa aacctacatt
     3901 tgataaacca gatgttcctt tagctaaaga tgccttttca atttcagatg tctataataa
     3961 gtacttcggt aatgcaagtg ttgatgcaaa cactggtatt gttacattca ctccagctaa
     4021 aggtgtagga gaatcggagc cgattactgg aacaattcct attaaaattg tttaccaaga
     4081 tggttctgta ggcacgaccg atttagcagt aactgtaagt aaagatattt atgaaaatcc
     4141 aggagaaaac attcctgcag gctaccacaa agtaaccttc accgcagg
//
LOCUS       DAYYFD010000152         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000152 DAYYFD010000000
VERSION     DAYYFD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.157"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHA25_002807"
     CDS             85..1434
                     /locus_tag="ACHA25_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8764040.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHA25_002808"
     CDS             complement(1562..2152)
                     /locus_tag="ACHA25_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8764041.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHA25_002809"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHA25_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8764042.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAYYFD010000153         4065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000153 DAYYFD010000000
VERSION     DAYYFD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.158"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1155)
                     /locus_tag="ACHA25_002810"
     CDS             complement(<1..1155)
                     /locus_tag="ACHA25_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8764043.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..3407)
                     /locus_tag="ACHA25_002811"
     CDS             complement(1158..3407)
                     /locus_tag="ACHA25_002811"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8764044.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3504..4007)
                     /locus_tag="ACHA25_002812"
     CDS             complement(3504..4007)
                     /locus_tag="ACHA25_002812"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8764045.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1310 a          743 c          580 g         1432 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgatatctt aaaaatgtaa cttatctcct gattaaacaa atgaaattgt
     3361 tggttttctg atcgataaat aatattcatt tcattatcct tatacataag ctctcctccg
     3421 tttctttatt gcattataaa aaacgctcac ataaatagta agcgtttaaa gtaacgaata
     3481 tttatcaaaa tcaaatgttt agtttattca ccattaatga ttcctagacc taatctacgt
     3541 tttcgatata ctcccggtga aacaccattt gctttcttaa aggctttaga aaaagattgt
     3601 actgttgaga atcctatctt gtcactgatt tgctggattg aatattcagt tgatgataac
     3661 agatcgtttg caatagaaat tttgatttca ctaatatact cttttattga tattcccata
     3721 tcattcttaa acaatcgtga taaataattt tcgtgtatcg atagactgac agcaatatct
     3781 ttaacagata atttttgata cgcattctca cgtattattt caattgtttg acgaacaaaa
     3841 ggattcatct ttttagtaat tatcccttcg ttcagataac tagctgaatt taagaggagt
     3901 tctaaaaatt tataaacttg tttttgtctg gacaattcac tgactagatt gtcctgatct
     3961 ttcagctgag tgttaggttc tgttactaaa tatatagaat tttccatatc caccctccta
     4021 tgttagattt ggataatttt tgttttatta tgtttatatt attac
//
LOCUS       DAYYFD010000154         4062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000154 DAYYFD010000000
VERSION     DAYYFD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.159"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1465
                     /locus_tag="ACHA25_002813"
     CDS             41..1465
                     /locus_tag="ACHA25_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8764046.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1646..2419)
                     /locus_tag="ACHA25_002814"
     CDS             complement(1646..2419)
                     /locus_tag="ACHA25_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8764047.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2416..3321)
                     /locus_tag="ACHA25_002815"
     CDS             complement(2416..3321)
                     /locus_tag="ACHA25_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8764048.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3475..3795)
                     /locus_tag="ACHA25_002816"
     CDS             complement(3475..3795)
                     /locus_tag="ACHA25_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8764049.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1218 a          751 c          815 g         1278 t
ORIGIN      
        1 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
       61 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
      121 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
      181 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
      241 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
      301 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
      361 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
      421 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
      481 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
      541 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
      601 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
      661 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
      721 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
      781 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
      841 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
      901 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
      961 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
     1021 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
     1081 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
     1141 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
     1201 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
     1261 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
     1321 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
     1381 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
     1441 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
     1501 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
     1561 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
     1621 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
     1681 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
     1741 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
     1801 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
     1861 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
     1921 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
     1981 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
     2041 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
     2101 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
     2161 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
     2221 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
     2281 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
     2341 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
     2401 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
     2461 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
     2521 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
     2581 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
     2641 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
     2701 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
     2761 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
     2821 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
     2881 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
     2941 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
     3001 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
     3061 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
     3121 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
     3181 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
     3241 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
     3301 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
     3361 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
     3421 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
     3481 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
     3541 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
     3601 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
     3661 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
     3721 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
     3781 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
     3841 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
     3901 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
     3961 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
     4021 caatttcata gaaagaagtt gttatttatt aatcatttaa aa
//
LOCUS       DAYYFD010000155         4039 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000155 DAYYFD010000000
VERSION     DAYYFD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.160"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..555)
                     /locus_tag="ACHA25_002817"
     CDS             complement(97..555)
                     /locus_tag="ACHA25_002817"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8764050.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(726..1871)
                     /locus_tag="ACHA25_002818"
     CDS             complement(726..1871)
                     /locus_tag="ACHA25_002818"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8764051.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1884..2801)
                     /locus_tag="ACHA25_002819"
     CDS             complement(1884..2801)
                     /locus_tag="ACHA25_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8764052.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3229..3792
                     /gene="efp"
                     /locus_tag="ACHA25_002820"
     CDS             3229..3792
                     /gene="efp"
                     /locus_tag="ACHA25_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8764053.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1264 a          758 c          769 g         1248 t
ORIGIN      
        1 ttttcccgtt tagaactctt ttcagacaat aaatatttaa taaaatacga agataagata
       61 gctatattgc ttctcatctt tacgttcttt tagaaactat gcttctactt cagaccattc
      121 atcgcgtttt gctaaaaatt cacgagccaa agcttgtgca ccttctagtg tatggctggc
      181 agcccagcca cattgtgttt cattgcttgc aggtacttca gtagcttcaa gaacatcttt
      241 cattgttgct tcaagaacat ctaaaatgtc ttcgtagctt tcatgattca atacagtcaa
      301 gtaaaagcct gtttggcatc ccattggaga ccagtctacg atgtaatctg cgtgattgcg
      361 gattagttcg gcagtcaagt gctcaagtga atgtagactt ggcatgtcca tgtgttcttt
      421 atttggttgt ttaaagcgga cgtcgtattt aatgatgaca tcgccatgcg cacccgtttt
      481 tcgatcagct acacgtacat aaggtgcttt gacttttgta tggtcaagat taaaactttc
      541 gacattcatt ctcatttgta agactcctat ctgtttgttc attcgttttg atgcttttca
      601 ctaaagaatt atttagtgaa atttacaaaa acgtctttct acttgattct aacacttatc
      661 gtttccaagg tgtagcaatc tgaaatattt tttagatttt tctgctaagt cgtttattgg
      721 gacttctagt tcttgattct gtcaaatgcc tgagctaaat cgtttatgag atcctgacca
      781 tactcaattc ctacagaaag acggatcaag ccgtccttta tgccagcctc ttctcgtttt
      841 tcttttggaa tagacgcatg agtcatgaca gaaggaattt ccactaaact ttctacgcca
      901 cctaaacttt ctgctaaagt aaatagttgg agtgattcaa caaatggaat agcagattct
      961 tcatttttta aagtaaaaga aatcattccg ctaaagccgc tcatctgctg cttagcaact
     1021 ccatggagtt catgatcggg taatcctgga tagtagacct tttctactgc tggatgagaa
     1081 aatagaaaat cagctaccac aaaagcattt ttttgatgtt cctccatacg aacacttagt
     1141 gtcttgattc cgcgctgaag taaccagcta tcttggggac caagtacggc tccgatagca
     1201 ttttggtaaa atccgatctg atcggctaga tctttatggt tagaagtcac taaaccagct
     1261 actacatcgc tgtgtccgcc tagatatttg gtaccgctat ggacaacgat atcagaaccg
     1321 agatctaaag gtctttgaaa ataaggagta gcaaatgtat tgtcagcaat aactatcaat
     1381 ccatgttgat gagccagagt ggcacttttt tctaaatcag taatctttag aagcggattg
     1441 ctaggtgttt caagataaag tgcttttgta ttgggtttga tggattgttc tattttatca
     1501 agattactcg tatctatgat cgtgtactcg agaccatttt tagtaagaac tttgtcaaat
     1561 agacggaatg ttcctccata gacatcatct cctaataaga tatgatcgcc agcttgaaaa
     1621 agagaaaaaa cggcatgaat cccagaaaga ccagaagaaa aagcgaatcc tcgaacaccg
     1681 ccttccaaat cagcaatcag ttcttccaat gcaaaacgag taggattacc agatcttgaa
     1741 tattcatatt gtttaggctg gccgacgcca tcttgccgat aagtggatgt ttgataaata
     1801 ggtacactga cagctcctgt tgtgggatct tcactgatgc ctccgtggat caattttgtt
     1861 tgaatgtgca tgtttctttc ctcctataag taaatgtttt ttgataaata gcggtctgct
     1921 gcgtctggaa aaatcgtaac gacacgatga ccaggtggta aacgacgaat ttctcttaat
     1981 gctgctgcaa aagccgcacc actcgaactt ccaactaata gtccttgttc tcgtgctaac
     2041 tgcctagtat agttaaaccc ttctacatca gagattgtct cgatctgatt gattgaaagg
     2101 ggagaaagaa acggcggaat gaattctaca ccgataccct caatttcgtg aggggctgga
     2161 tcaccaccat ttaacacaga gccctctggt tctacaccga ttattcgaat gtcgggatat
     2221 ttttctttca aaaaggtaga agttccagca aaggttccac cactcccaat tcctgctaca
     2281 aaactatgga tattttcttt gatttcatga aagatttctg gccctaacgt atgatagtaa
     2341 gcagcaggat tatctttatt ttcaaattga agaggcagat agctgtttga tatctcctct
     2401 gcaagttttt tacttttgct gatggcaccg atgattcctt gctcactagg agaatggatg
     2461 atctttgctc ccaatgcttt catcaattgt tgtttttcta agctaaactt ctctggaaca
     2521 acaaaaaccg ttcttaattt gtatgtgagt gcggccagag cgatcccaat tcctgtattt
     2581 ccggcagttg gttcgatgat cgtcgttttt tcattgattt ttccttgggc aaatccttct
     2641 ttgatcaaat aactgcccaa tctatctttg atacttcctc caggatttaa atattctagt
     2701 tttgcataaa tagcagaacc tttaggcata gaatagtgct gattgtcaaa gcgataaagc
     2761 ggtgtggaac caatagcttc tgtaattgtc gagataatca tgtgggcctc ctaaaaaaag
     2821 ataataaaaa aaggagctga ctattaaatc agctccttga aagacataga gaaaaattga
     2881 gtagataaaa agataagtta cctggtactc attctatgaa gggcaattaa tagacatgac
     2941 aaatagtatg ctgcttttat gcaccataca acagcaacga ttcaattgtt tttggttgct
     3001 gactgtcatg tgtatccctc caagtaaaat ttttattaac gatagcatga aaaaaaataa
     3061 aagtcaaagg cttgaaagaa aaaaatattt cgatagcatc gtttacccgt gtttattttt
     3121 ctgacaaata ggcatgagac cttaaaagat agagaatact ctagttttct ggccggttgt
     3181 ctgatacaat aaagttgatt atgataaaga aaagaaggag caaaaataat gatctcagca
     3241 agtgatttaa gagccggtat gactttcgta caagacggca aactgattaa agttctggat
     3301 gcaagccacc ataaacctgg taaaggaaat acagttatgc gtatgaaact gaaagatgtc
     3361 cgttcaggtg caacatacga cacaacgttc agaccagaag aaaaatttga aaaagcacat
     3421 atcgacacaa aacctgtaca atatttgtac acgatggatg atacagcttt cttcatggat
     3481 ttggaaacat atgagcaata tgaaattcct gtagaaacag tagaagaaga aatgaaatat
     3541 atcctagaaa acatggaagt aaaaatccaa tttttcggta gcgaagtcat cggcgttcaa
     3601 ttaccaacaa ctgtcgtatt acgtgttgtg gaaacacaac cttccatcaa aggtgcaaca
     3661 gtcactggtt caggtaagcc agcgacaatg gaaactggct tagtagtcaa tgttcctgac
     3721 ttcatcgaaa cagatgaatt gttggaaatc aatacagcag aaggttctta tgtaaaacgt
     3781 gcgtcaaaat aattcgctaa cggattgata agaatttgaa aacagagctt gacctcttga
     3841 aaggcagatc atcggatctg cctttcaaga ggttttttgt ggaatcagca aattgaaaaa
     3901 taaaaaagta tttgatttta tttatatttt gaaagatgaa tggcaaaagc tagagaaaga
     3961 aaaaactgca cttatttatg aagaaattga aatgattcat aaaattgaaa atatccgaac
     4021 tattccgaac tatattgaa
//
LOCUS       DAYYFD010000156         3878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000156 DAYYFD010000000
VERSION     DAYYFD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.161"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACHA25_002821"
     CDS             complement(718..3867)
                     /locus_tag="ACHA25_002821"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM8764054.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAYYFD010000157         3753 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000157 DAYYFD010000000
VERSION     DAYYFD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.163"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..316)
                     /locus_tag="ACHA25_002822"
     CDS             complement(152..316)
                     /locus_tag="ACHA25_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764055.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            611..1939
                     /locus_tag="ACHA25_002823"
     CDS             611..1939
                     /locus_tag="ACHA25_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8764056.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2141..2974
                     /locus_tag="ACHA25_002824"
     CDS             2141..2974
                     /locus_tag="ACHA25_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8764057.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2990..3727
                     /locus_tag="ACHA25_002825"
     CDS             2990..3727
                     /locus_tag="ACHA25_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8764058.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
BASE COUNT         1254 a          590 c          784 g         1125 t
ORIGIN      
        1 tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt
       61 ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc
      121 tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata
      181 cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga
      241 cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag
      301 caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa
      361 tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac
      421 aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt
      481 atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata
      541 gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa
      601 ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca
      661 agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc
      721 tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac
      781 ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac
      841 attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta
      901 cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac
      961 aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga
     1021 tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc
     1081 ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa
     1141 attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga
     1201 cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc
     1261 attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca
     1321 tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga
     1381 tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac
     1441 agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat
     1501 ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg
     1561 ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc
     1621 aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg
     1681 agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc
     1741 aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt
     1801 tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt
     1861 gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc
     1921 aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga
     1981 gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg
     2041 tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg
     2101 atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt
     2161 atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac
     2221 ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa
     2281 aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc
     2341 ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga
     2401 gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac
     2461 agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc
     2521 ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt
     2581 ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga
     2641 acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct
     2701 gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt
     2761 ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc
     2821 ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact
     2881 gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg
     2941 ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa
     3001 atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt
     3061 tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag
     3121 aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga
     3181 gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta
     3241 caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt
     3301 caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa
     3361 tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa
     3421 aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga
     3481 tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata
     3541 tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga
     3601 gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac
     3661 aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa
     3721 taaataaaaa agaggttgtg acagaagggg gca
//
LOCUS       DAYYFD010000158         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000158 DAYYFD010000000
VERSION     DAYYFD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.164"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHA25_002826"
     CDS             complement(<1..353)
                     /locus_tag="ACHA25_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764059.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHA25_002827"
     CDS             complement(613..1161)
                     /locus_tag="ACHA25_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764060.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHA25_002828"
     CDS             complement(1162..2019)
                     /locus_tag="ACHA25_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8764061.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHA25_002829"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHA25_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHA25_002830"
     CDS             complement(2581..2910)
                     /locus_tag="ACHA25_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8764062.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHA25_002831"
     CDS             complement(2987..3259)
                     /locus_tag="ACHA25_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8764063.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHA25_002832"
     CDS             complement(3259..3522)
                     /locus_tag="ACHA25_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8764064.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYFD010000159         3713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000159 DAYYFD010000000
VERSION     DAYYFD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.165"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..152
                     /locus_tag="ACHA25_002833"
     CDS             69..152
                     /locus_tag="ACHA25_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8764065.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="ACHA25_002834"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="ACHA25_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8764066.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1277..2140)
                     /locus_tag="ACHA25_002835"
     CDS             complement(1277..2140)
                     /locus_tag="ACHA25_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8764067.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2142..2414)
                     /locus_tag="ACHA25_002836"
     CDS             complement(2142..2414)
                     /locus_tag="ACHA25_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8764068.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2431..2640)
                     /locus_tag="ACHA25_002837"
     CDS             complement(2431..2640)
                     /locus_tag="ACHA25_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8764069.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2718..3614)
                     /locus_tag="ACHA25_002838"
     CDS             complement(2718..3614)
                     /locus_tag="ACHA25_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8764070.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1140 a          652 c          575 g         1346 t
ORIGIN      
        1 ttttgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctacc gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttata gatgtaatca cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag ataaattatt
     1081 aggtataggg cacctctaat aactaccaat taattcacct caaaatgaaa ttagaaaaaa
     1141 acagcgaaat ttttaaatct atttcttatc gatacaaatt cctcgtaggc gctcgggacc
     1201 ccaacatgag gaaatgtttt tataaaaaaa gagtagttac cgttttttgg taactactct
     1261 tttccttttt taaggtttaa atacctggaa gtttaggtgt tttgggtatt ggtggtgtag
     1321 gtagctgcaa gctttccatt ttttgttgaa ttgctttaaa ttcaggggtt tcttggtgtt
     1381 gattttgaac cattttttgc tctattctct ccaaagtcgg ttgaatttct ttccctgata
     1441 ctttacgatt taattctctt tctaaggtct ctttcggact aatggaaggc gtttctaagc
     1501 ttgaatagag tttcactcct tctctattgt aaagccttat atcgctaaat aagcccgttt
     1561 tatgaagggt ttctaaattg gtcggtaagt ttttgacaac aatatcatgc gcttgttttg
     1621 gtgttgccct agctgtcatt ggatcatctg catacatggt ttcatatcgt tcaatcgttc
     1681 ctaagtatga ttcaattttc ggtactgcca tgacgtatat ttttgtttca taacctttag
     1741 attgaagcat tgtggctgtt tgaatgggaa cgtctgttgt tcgtcctgtt ccttcaatca
     1801 ctaaattata gccttgatcg cttaaacggc taataagtgc ttctgtcatg cgattcgaat
     1861 aaggggtaac gtgttttact acgtcttttt catagagttt cactaattcg tcaaaatttg
     1921 ggtgctgttg tttaaaggta tcattatcaa tgacaataac attcccttgt gtttcttcaa
     1981 aaattgctga tcgcaaactg gttttccctg accctggttg cccaccaagt aaaaaagcgg
     2041 ttggcgattc aaccgctagt ctatttttag ttagtcgttc aacattcttt tctaaacgat
     2101 cttcaaattg cttttcggtg aagtcagtaa tattcgccat gttaagccac tttctcttta
     2161 ttcaaaattt gttttgaata ttgattcatt gaccgccaat actcatgaat agcttgtaat
     2221 tcttcaaatg gttgttgaaa taaatcaact tcttgtgcca cttctaattg atttaaaaga
     2281 ttcacttcta gtaaacgagt atttttagta tctagttcat gattcaaaac ataatttaat
     2341 acccgattat aaacacttgc cgataactca ttaatgattt caatttcaaa tgttttttca
     2401 tacgtaactg ccatttcatt ttcactcctt ttaaagtttg tcaggtaaat atttccgtat
     2461 atattcttct aaggcttgat ccataacttc ttttacgttt ccgccattct ttgctgtttc
     2521 gatttttatg atatggtgca agtcggcacg aacacgaacc gtcttatccc ccattatatc
     2581 tttttttgcg ctgattggcg tatcatttcg ttttgctttc tgtgcgccta aatttcccac
     2641 gatcgctcac ttctttctat ttcttcttac tatttatttt atcatcacct atcacaaatc
     2701 acaagtgatt tgtgattcta ttctttttca ttttctaatt gaatgattcg ctcaatcatt
     2761 tcaaaaaata cgttctcata catagacaaa acttttttgt catagccttt gtgttctgta
     2821 atgccatttt tagaccaagt acttacttta ttacttcgct tgataatatt tcggaaaacc
     2881 aaattatcct ctttatgttc tttgtacagt tcttccaggt ttgattttat cgttgcgctg
     2941 tccgtatcaa ctaaataagg aacaaaacca atcatatcta gtccagggtt aaactgttct
     3001 tgcaaatcaa tcaaatagga aatatagttt tgaatgttgt ttgtactttc ttcttctgct
     3061 tgtaaaggga tcataacgta atcactcgcc acgattgcat tatttgtata aacgcttggc
     3121 gttggtacag tatcaataat aataagatca tagtcacttt ttaaaggtgc taaaagagta
     3181 gcaagcaatc tactttcatt ttcaaacgtc catgagcgag ttaattttgg cagtaacatc
     3241 aaatcaaacg tgccagggat caagtctaaa ttatcagtca aatgaataat agaagaagcc
     3301 aagtttccat ttttcaagcc ttcataaaaa ttgacacgtg gcaattctac ctcaaaagtt
     3361 tttgctaagt cttttgtcaa tgttgcctgt aagtccttat cgatcattaa aacttttaaa
     3421 ttcaatttgt ctgtcaagta agcaaacata gtcgataatt tggactttcc aacaccacct
     3481 ttaaagtaat tattcaagat aataattgct tcatttttat tgtttaaaat acgtcttaat
     3541 tcttcaagta tttttagttt ttccttctcc ataactacgc cccattcttt ttttgtatag
     3601 taatattgta tcattaagaa tcacaaatca caagtgattt gtgattaatc acttgtgatt
     3661 tgtgataagt gatttgtgat taatatataa aagccctctt taaagggctt tta
//
LOCUS       DAYYFD010000160         3557 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000160 DAYYFD010000000
VERSION     DAYYFD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.166"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1867
                     /gene="yfcC"
                     /locus_tag="ACHA25_002839"
     CDS             128..1867
                     /gene="yfcC"
                     /locus_tag="ACHA25_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8764071.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2024..3208
                     /gene="iadA"
                     /locus_tag="ACHA25_002840"
     CDS             2024..3208
                     /gene="iadA"
                     /locus_tag="ACHA25_002840"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8764072.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3319..3522
                     /locus_tag="ACHA25_002841"
     CDS             3319..3522
                     /locus_tag="ACHA25_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8764073.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1136 a          592 c          823 g         1006 t
ORIGIN      
        1 acaaaataga aaaaagtgat tttctaataa attattcatg tggaaatgtc atttttactg
       61 tataattcat ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga
      121 aataaatttg aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc
      181 gacaccccat acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt
      241 tgtcgttcca gcaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga
      301 aacaagtact cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa
      361 gtcgtatgtg tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact
      421 ggatgttccg gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca
      481 ggaaaaacta gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa
      541 tatctatgat acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt
      601 tggcggcttt ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg
      661 atctgctgta gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat
      721 gcgtaccgga gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga
      781 gcgtttagct ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat
      841 ggcagaagag gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata
      901 tgactcgatc gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc
      961 ttggatgagc ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc
     1021 tggtgcgaca ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat
     1081 gatgatatat gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga
     1141 tgattatttc cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg
     1201 gcataaattg atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac
     1261 tcaaaaaggc tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc
     1321 tggaatcatt gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc
     1381 tttccaatct ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg
     1441 gattcttctt gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt
     1501 gcatgggatc gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta
     1561 catcttccaa agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac
     1621 tatgccaatc atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct
     1681 tgcttatcag cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat
     1741 tggtgtgcta ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat
     1801 gcaaggattc ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct
     1861 gcagtagtac tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa
     1921 gaaccgtcat tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt
     1981 aagatcctat catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca
     2041 aattgaagtg tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa
     2101 gattttggct gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact
     2161 ttcaggtgag ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg
     2221 tggaggagga gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac
     2281 tacagctggt gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat
     2341 gactgcgttg atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata
     2401 cgaaggttcg taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt
     2461 gacaattgat aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca
     2521 gccaagtttt gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc
     2581 cggaaaagct gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt
     2641 gacacgaatc gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa
     2701 tcgcacacca gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga
     2761 cttcactgct agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag
     2821 caaagcactg aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc
     2881 agatggacaa ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt
     2941 aggaagtgcc aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc
     3001 tttggaaatc gccttacgag ctataaccag taatccagca cgtatattga aattggataa
     3061 aaaaggaaaa atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct
     3121 tgatattgat acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt
     3181 atgggggact ttcgaaaaaa gtttttaatc aacagctcta actatctttt ttagaaaacg
     3241 agcgttctgt ttgtctgtat ctgttgttga tgttaccctg aaggtagatc aataaacaaa
     3301 ctaagaggag gaagcacaat ggcagattta aaaggacgtt ctaaagatat gaaagataaa
     3361 gtagtcggtg aaggaaaaga agcatacggc aaagcaacag acgacaagag caaagaaaca
     3421 gaagggaaag cacaatcact aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac
     3481 gaagtcaaag acaagttttc tcgaaaagac gaaaaaaaat aaaaaccact tattactaat
     3541 tgaatcaagc aaataaa
//
LOCUS       DAYYFD010000161         3555 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000161 DAYYFD010000000
VERSION     DAYYFD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.167"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(186..863)
                     /gene="nth"
                     /locus_tag="ACHA25_002842"
                     /pseudo
     CDS             complement(186..863)
                     /gene="nth"
                     /locus_tag="ACHA25_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(870..1568)
                     /locus_tag="ACHA25_002843"
     CDS             complement(870..1568)
                     /locus_tag="ACHA25_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8764074.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1836..3020
                     /locus_tag="ACHA25_002844"
     CDS             1836..3020
                     /locus_tag="ACHA25_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8764075.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3026..3469)
                     /locus_tag="ACHA25_002845"
     CDS             complement(3026..3469)
                     /locus_tag="ACHA25_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8764076.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          913 a          716 c          596 g         1330 t
ORIGIN      
        1 tacatcgggt attttcccaa taaaaataac aacttttatt tttatcgcaa taaattgtaa
       61 taagttacca tcctagctac tgagtatgta catttacttt ttcatgcaaa aaagataaac
      121 gaaacagctt ttctccataa taacaagata attcatacat ttatttgcaa ttaaagaaac
      181 cacgtctaca ggcgtggttt cttttttagc gccttttctt ttagacgcat ccgatttttc
      241 ccatcttgac atattgatag caaaggacat acctcgcact tgggatttct tgccgtgcaa
      301 tgatatcaac caaaaaaaat cagtgtatga tgtgtcttta cccataattc ttgtggtact
      361 ttccgcatca gtgtttcttc tacttccatg accgtagcat ctagtttgca gatcctcagg
      421 cgttttgaga cacgttccac gtgggtatct actgcgattg ctggtatccc gaatgcatcc
      481 cccaatacga catttgctgt ctttctgcct acaccaggaa gagacatcaa ttcttctctt
      541 gacgttggca cttgcccatc gaaacgttca atcagctgct gtgcacacgc tttgatgttt
      601 tttgctttat ttcgatacaa accaatcgtt tttattttaa gaatgatttc atcgattgaa
      661 gcttctgcta acgcatcggg tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc
      721 actgatacat ctgtcgcctg ggcacttaag atcactgcaa tcaataactc aaatggatta
      781 ttgtgcttga gttctccatg tgcttcggga aacattccat acatcgtttc tagagcttcc
      841 attgtttttt gtttgcttaa catattccgc tactcctcca accaattttt cagtgagact
      901 ttcggcaatt cttcttgttg agctgactgt tctacttctc tttgaagaag ctgctgtttt
      961 cttcgttttt ggtcttcttc cacttgttgt tttgtacgta gattttttct ttcccacgat
     1021 agcaaaatgc ggtcaacgta attaaaacta taagcctgat ttaatacggc ttcccgcagt
     1081 gctagcttca aaatttccgg ctgataatgg tcttcttcta gccattgtcc gatccgctgg
     1141 aactcgatcg aagaaagcgg gcgtccaaat tcttgttcaa ataattgata cactgactgg
     1201 atttcttttt cttgtgtttt ttcttcgtat tttttttctt gtgtttttaa atactcacct
     1261 aatagatcat agatggggta aagattatat cgatctgctt ttttaccgtt gacaatcgtc
     1321 gtttctatct tgataaatcc atttgttacg agacgatcca gtatttgaaa aatctgatct
     1381 tgtttgattc ccatatccat agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc
     1441 tgtgccatat gcaactgtag aacaaataaa aattcttcag attgtagacc gatcctatga
     1501 taatttttaa gtaaaagatt agatatgact gtttgtccag cgcttaagta attttctaaa
     1561 ttcaacatga tttctcctcc gctcaatcac agtatacgta aaaaaaacat gggaagcaag
     1621 aacaggtttc ttttctattt acttcactga tcggtcagat ttacaatgtc gtctacttga
     1681 tttttgtttg ttcatgtttt tgatgcgtat cttcactttt cggaataaat aaattaagta
     1741 gatttcgctg ttccttagaa tgttgacaag gaaacaaaat tactatacac tatttttgtt
     1801 tccaagttaa cattattgca taaaggagtt tttacatgta cagtttcaca aataaacatt
     1861 ctaaagtttt tggatacgtc tctgtcttat tcactggatt aggatttact atcgtcagtc
     1921 ctgtacttcc gtttttaacg ctgccttatt ctcactccat acatcagcaa gctttctata
     1981 tcactttgct catgtctgtt tatgctctag ctgccttctt atctgccccc attctcggta
     2041 gcttaagtga tcattttggg cgtcatccga tccttattat cagtttatta ggatcaagta
     2101 tcggatattt gctttttggt ttaggaaatt cgatatggat gttgttcctt ggtcgtatca
     2161 ttgaaggatt gacggcagga gaaatctcca ccttatatgc ttactttgcc gacatcacgg
     2221 aacctaatga aagaaccaaa gtttttggat ggatgggtgc tttagtcggt ataggaacaa
     2281 cgctgggtcc tattataggg ggtctattag ctgagttggg gaattctgtt cctatattta
     2341 ttggagcact ctttactttc ttaaatgccg tttatggcta cacatttatg ctagaaagcc
     2401 ttccaataaa aaaacgttca gtagatttaa gtttctccca tgtaagacct tttcaccaat
     2461 tacaacaatt attcaagatt tcatccgtta ttcctttatt gacagcaggt tttgctgttt
     2521 ggttggcagc tggctctctt caatctatct tttctcaatt ttcgattgac gcctttcagt
     2581 ggaaggctgg gttggttggt ttaagcttct ctttgattgg aatcttggat actgtctcgc
     2641 aattgttcat tatgcctcga ttgctcaaaa aattttcaga acagcagatc acaagaatag
     2701 ggatgttcag tgaaatcctc gcctatctat tcatcacatt atctggaatt cttctactgc
     2761 cgattttgtt tttatttggg attatctgtt atggtttcgg tgattctatt tttactcctg
     2821 tattcaatgg ccaactatcc aattctgtct cagataacca gcaaggtctt gtaatgggcg
     2881 gtacccaaag tatacaatct ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt
     2941 atgctgcagc accatttctt ccgacggtat tcggattttt gcttatgatt tttgcctatt
     3001 atcactacaa taaaaaatag tagcttcatt tgctacttcg tataaattct ctgtatcgac
     3061 cgataaaacg aaatactgtg tcaaattctt cttttgacat attttcaaat acagtagcat
     3121 ctcgctgtac ccatttttga tggagttgct cgtgtctatt ccttattttt tgtccgattg
     3181 gcgtcaattg aaaatagatt tcttttttat tctcttcgct ttgataagtg tttatgatcc
     3241 ctctagctat cagttttttt gttagcttac tgatggctcc tctagtcatg taactagcat
     3301 tcgctaattt tgtcacgtta ggatcaggga ttttcccgat atgttcaatc acttctagtt
     3361 catttagagt aaatcctgca aattcttttt ccatttctgg tttattacga tgaataatct
     3421 cattagctaa atccagatat tcttctatta gcagttgtct tttatccatt tctacacctt
     3481 ctttttcttt cattatattg tttttttgtt tcctattcaa caaagataaa aatattttgt
     3541 aggaaaaccc aatct
//
LOCUS       DAYYFD010000162         3517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000162 DAYYFD010000000
VERSION     DAYYFD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.168"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..1016
                     /gene="galE"
                     /locus_tag="ACHA25_002846"
     CDS             27..1016
                     /gene="galE"
                     /locus_tag="ACHA25_002846"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8764077.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1028..2512
                     /gene="galT"
                     /locus_tag="ACHA25_002847"
     CDS             1028..2512
                     /gene="galT"
                     /locus_tag="ACHA25_002847"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8764078.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2536..3516
                     /locus_tag="ACHA25_002848"
     CDS             2536..3516
                     /locus_tag="ACHA25_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8764079.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1189 a          642 c          775 g          911 t
ORIGIN      
        1 ttttactaat caaaggagtg ttttttatga caattttagt actaggcgga gccggttata
       61 tcggttcgca cgctgtagac cagctagttc aaaaggggta tcaagtagct gtggtagata
      121 atttgctcac aggtcacaaa caagcagtac atccagatgc tcatttttat gaaggagaca
      181 tccgggataa agaatttcta cgttccgtat ttgaaaagga accgattgaa ggagtaatcc
      241 attttgctgc aagctcttta gtaggagaat ctgtcgaaaa accattgatg tatttcaaca
      301 acaacgtata cggtatgcaa atcttacttg aagtcatgca cgagttcaat gtgaataaaa
      361 tcgtgttctc ttctactgca gcaacttacg gagaaccaaa agagtctcca atcacagaag
      421 atacaccagc taatcctaaa aatccgtatg gtgaaagtaa attgatgatg gaaaaaatga
      481 tgaagtggtg tgaccaagca tatggcatgc gctatgtcgc tttgcgctac ttcaatgtag
      541 ccggagcaaa agcagatgca tctatcggag aagatcatac accagaaacg catcttgtcc
      601 caatcatttt acaagtagct cttggtcaaa gaaaagcatt ggctgtttat ggagacgatt
      661 atgatacacc agatggaaca tgtatcagag attatgttca agtagaagat ttgattgctg
      721 cacatatttt agctttggaa tacttgaaag aaggaaatga aagcaacttc ttcaatttag
      781 gcagcagcaa aggatactca gtcaaggaaa tgctagaagc agctagagaa gtgacaggaa
      841 aagaaattcc agcagaaata gcaccaagaa gagctggaga tcctagccga ttagtcgctt
      901 caagtgaaaa agcgagagag gttctgggtt ggaaacctga atacacagat atcaaagcaa
      961 ttatcaaaac agcatgggat tggcatgtca gccatccaaa tggttatgaa gaatagggga
     1021 atgagatatg tcaatcagtc aaacgatttg tgattttgcc acacttgcga tcgaagctgg
     1081 cggatggatg gaaatggacc gcttatattt acagaatcgt cttttaggga tggtaggaga
     1141 agatgctcta gaaagcattg aagtaagacc agttaaggaa gaatctgtag acttattgga
     1201 tcagcttgtc gctaaggcaa gagaaaatgg tgttctttca gaattgccag cagaacaaga
     1261 gatttttgaa gcgcaattaa tggattttct cacaccgcca ccttcagtcg tcaatgcatt
     1321 ctttgcacag cactattcga aaagcccaga ggaagcaact gagtatttct ttaatttaag
     1381 taagaaaaat gactacatca aaacaagagc catcgcaaaa aacatcaaat ttccggcaga
     1441 gtcggagtat ggtgaactgg aaatcacaat caatttatct aagccagaaa aagatccaaa
     1501 acaaatcgca gcagaacgcg aggcggtaaa agtagactat cctacctgca tgctttgcat
     1561 ggaaaacgaa ggatatagag gtcgcttgaa ctatccagcc cgcaccaatc accggattat
     1621 tcgaatgaat ctagacggtg aaagctgggg attccaatat tctccttatg cttattacaa
     1681 tgaacattcg atcattttat cagaacaaca ccgcccgatg aaaatcacaa aggaaacatt
     1741 tgctagactg cttcggatta ctgaagtctt gcctcattac tttgttggtt cgaatgctga
     1801 cttgccgatt gttggaggat cgattctttc tcatgatcat tatcaggcag gacgtcatga
     1861 atttcctatg gcaaaagcac cgatagaaaa acttatcaaa ctagcagatt atccgcaagt
     1921 catcgcagga gtcgtgaaat ggccgatgtc tgtgatccgt ttgcaatcaa cagagaaaaa
     1981 attgttgatt gaagcagcta actcagtatt ggaaaagtgg aaagcatatt cggatgaaac
     2041 agtatctatc agagcttttt cagcagatgg tacaccacac catacgatta cgccaattgc
     2101 acgaagaagg gacaattact ttgagttgga ccttgtcctt cgagacaaca atgtttcaga
     2161 agaacatcct gatgggattt ttcatccaca taaagatgtc caacatatca aaaaggagaa
     2221 cattgggttg atcgaagtaa tggggcttgc tgttttacca cctcgtttga aaccagaact
     2281 agtagaagta gagcatttct tattaggaga agaaaaccag atggctgaat atcatcgtcc
     2341 gtgggcagaa caattaaaaa aagaacatcc tgatttgacg aaagatacgg tcaccgatgt
     2401 cgtacaaaaa tctgtaggtc aggtttttgc acgtgtactg gaagacgctg gagtattcaa
     2461 gcgagatgaa aaaggacagg cagcgtttga acgatttact gcaattttat aaacgtaaaa
     2521 aaggaggaga gacaaatggc aacaattaaa gacattgcgc agttggcagg agtttctcct
     2581 gctactgttt caagagttct caactatgat cctgatcttt ccgttggctt agccacaaag
     2641 caaaaaattt ttgaagcagc agaacgctta aattatacga aacatcgtaa aaatgcagca
     2701 aatactaagc aaacgcttcg cctgatccag tggtacgacg atacggaaga gcttgaagat
     2761 ctttattatt tatctattcg tctaggaatc gaaaaaaagg cagaagaagc aaacgttact
     2821 ttattgaaag aaacgtggga ttccattagt gatcttccga cagatggaac gatcgcacta
     2881 ggaaaattcg acgaagctca gttattagaa atgaaaggat cccaaaatgc cttgcttttg
     2941 gtagattctg acggcacttc tcaaggaatc gactctctag ttgtcgactt taagtcaagt
     3001 gttcaaaaag tcattgaaca ttttgtctcc cataaagcgc aacatatcgc tatgcttgct
     3061 ggaacagaat atacgaagaa aaaccatcag cggctaaaag atcccagaat tatggcattt
     3121 caacaagttt tggcagaaaa aggctacgac gaaggtccaa ttatcgaagc agatttttct
     3181 gtcgagtcag gatatcaagc agtgaagcag tatttaaaaa cgaatccgca tgttcctgat
     3241 gcgctttttg cagctaatga tgcattagcg attggcgcac tgaaagcgat tcaagaaaaa
     3301 ggtttagtgg taccagaaga tatttctgtg atgggtttca atgatgtcag tgtagcgaaa
     3361 tatgtaacgc cctctctttc tactgtaaaa gtttataccg aatggatggg agagctagca
     3421 gtggagacga ttcttcaatt aagtaattcc aaagcaccgg tttcaagaaa gattattgtg
     3481 gaaacagagt tgattattcg agaatcaacg aaataaa
//
LOCUS       DAYYFD010000163         3515 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000163 DAYYFD010000000
VERSION     DAYYFD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.169"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1359
                     /locus_tag="ACHA25_002849"
     CDS             28..1359
                     /locus_tag="ACHA25_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8764080.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1325..1675
                     /locus_tag="ACHA25_002850"
     CDS             1325..1675
                     /locus_tag="ACHA25_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764081.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1767..2186)
                     /locus_tag="ACHA25_002851"
     CDS             complement(1767..2186)
                     /locus_tag="ACHA25_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8764082.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2187..2531)
                     /locus_tag="ACHA25_002852"
     CDS             complement(2187..2531)
                     /locus_tag="ACHA25_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8764083.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2881..3477
                     /locus_tag="ACHA25_002853"
     CDS             2881..3477
                     /locus_tag="ACHA25_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8764084.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          923 a          640 c          641 g         1311 t
ORIGIN      
        1 taaagaaaat gaaaaagaag gtgtgtaatg gaaaacgaaa ctgacgttat ttatattcat
       61 cctcaaaaac gaatcgtttc acagaaaaga aagtattttt atcttggttt tacaggtgtt
      121 ttttttcttt ttattggctt gctttctaat actcctactg ataattggag tggattgcta
      181 actattctta cctcgccaag caatcttttg acagattatt ttgctttagg tgttttcgga
      241 agcgcattca taaatgtagg gattttgacc ttactttctg tgcttttagc ttatcggcac
      301 aaggtgattt taaacggtcc cttatttgct tcgattctaa cagttaccgg tttctctttt
      361 tttggcaaaa acctttataa ttctatctca atcattttag gtgtgtatct atacgctgta
      421 tttgtcaata aacctttttc tcagtacatc atgatcggac tgtttggtag cgtattatca
      481 ccggtcgtta gttatatcac ttttggtatg cgttttcctt tactgatagg tattttattg
      541 gggaatttag ctggcatagc aattggtata cttcttccgc ccttagctgc tcaaacttta
      601 gttttccatc gtggattcac actttataat attggattca cctctgggtt gattgcgatg
      661 acttttactg ctgttttgag gttattcagc tattcaatcg tcgaaaatac attggttttc
      721 aatgaatatc actttcctct catttggatt atttttggct ttttttcgct gacagtcgga
      781 attggtttct attataattc ctttcggctt tcggggattc gagaaatttt cgattcatct
      841 ggaaaattga caactgattt cattgctaat tctggtattg gtgctacttt gatcaacatg
      901 ggcttagttg gtctgatgct gtcttcttat gttttgctgg ttggcggtca gttaaatgga
      961 cctgtcatcg gtgctatttt atctgcggtg ggtttcagtg cttttggttg tcatttaaaa
     1021 aacagttttc cgatactagt cggaatattt attgcttctt tgtttggaac ttttcatgag
     1081 atcaccagta ctggcatgct tgttgcagct gtgtttggga caggtcttgc gcccataagc
     1141 ggtttttacg gcagttttta cggtgtaata gctggtgtgc tacatattgc tcttgtccat
     1201 aacgtcagca ctttgcacgg aggactaaat ctatataaca gtggtttttc aactggtttc
     1261 gttgcaggta tccttgtacc gatactagat aactttaccg ctgtcagaaa ggaaaaaaag
     1321 acacttggaa aaagaattat caaaaagaat catcgatgag cttcttactt atttatattc
     1381 ccatgaaata actgaaatac gtattggagt agaatttgct gaggaagggc ttttcataga
     1441 gatacagggt acggcagaaa ctcagccaga agatttagat gtacttgctg atttgctgaa
     1501 caatcctcgg gaccctagta tggaaatcta ctatcaagaa ctattaggta atccccgaca
     1561 tgatcgggaa gattatcatc tgttaggtag tttgattgat gacgtggaaa tcatgtatgc
     1621 ccagccgatt atcaatatta agatctttcg acgtgcttct cattcctatt attaacccta
     1681 ttttctattt ataaaagact gaatcaaaaa agcagctcct gaaagttcga ttaaaatcta
     1741 ctttcaggag ctgttcaagc ttgacattat tgattttcca atccttcata cacttcatct
     1801 gttacaggtt ctaaaaactc tgaattacca gcagtgatcg ccaaatgttc aaaccaactg
     1861 tcttttgtcg caccatgcca atgtttaatc cctttgtgcg ttacgacgac atctccagcc
     1921 attaatcgtc ttgctggctt tccttcttcc tgataccagc cttctcctcc ggtcacaagt
     1981 aagatttgat agccgtctgt atggacatgc cagtgattgc gacaacctgg ttcaaaagtt
     2041 acattaccta caccaacaga aacttcggga tcattcacta acatattcag atagctttgc
     2101 ccggtaaata cttgagcgta agcttcgttt ttttcacctg ctggaaaaat cacgccattg
     2161 tgtatttctt cgtgttttgt catcatttac gcctctttct tatcttcgct attttcttct
     2221 aaatagattt cttttgctaa actaaaagct gaccaagctt ttggccattc tacataaaaa
     2281 gctaaatggg taatgatttc tgctatttct tcactagtaa tgccattttg cttgccaatc
     2341 ttcagatgag ctggcaattg ttcaaaattt cctcctgaaa ttaatgcaga tatggtgatg
     2401 atactacgct gatgagcaga taattcttct ctggaccata cttgtccaaa taaaacatca
     2461 tcatttaatt ctgcaaattt tggtgcaaaa tctcctaatt gatctcgtcc tgcggtttgt
     2521 ttttctgcca tttctcttat tctcccttca cgactgttag gtgattcaac tatggttcta
     2581 aggcaacttt attttctatt gtttcttcgc atttcatttt aaatgtagtc tttcagcctg
     2641 tccaatgctt agtttattca gtcgatatgc tttttaagca tagttgctaa cgcttttttt
     2701 attttaaaaa aaacaagtta ttaaataagt ctttagttgg atgttttttt gagatcaatc
     2761 ttttatactt atgatgaact tacaaaattg tttgttttct gtcatatcaa aaaatggcgc
     2821 agttagttgc ttttctagta aagtatagtt aacaaagaac ctctaaggag ttgatgaata
     2881 atgaatattg ctgcctctcg aaaactgata aatttcatat ccattatcgg attaggccta
     2941 tcaatcgcgc tgacgattta ttttattaat ttaggtgttt tcaaagattt gaatgcgtta
     3001 agaggattag taggtgattc catcatttta ggaccgatta tttttgtctt catccaaatc
     3061 ctgcaagtag ttattccaat cattcctgga gggatcagta ctgcagctgg tgtcctgata
     3121 tttggccctt atgctggatt tatctataac tatattggaa tctgcattgg ttcgattatc
     3181 atttttctgc tagggcgtcg ttatggaaag ccattcatac tttccatgat tagcgataag
     3241 acatataaca aatatattgg ctggctggat aatcaaaacc gttttgagaa gctgtttgct
     3301 ctagctattt ttcttccagt tgctcctgac gatgcgttat gtttgatggc tggattgaca
     3361 aacatgtcag tgaaacgata tacactaatc attttgattg ctaaaccact gtcgattttc
     3421 ttatatagta tggccttgat ttatggaggt caatatctaa gtggtcttct tggataatat
     3481 ttattttgta aaattagtca ttgattgaca atgtt
//
LOCUS       DAYYFD010000164         3434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000164 DAYYFD010000000
VERSION     DAYYFD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.170"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHA25_002854"
     CDS             complement(<1..2232)
                     /locus_tag="ACHA25_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8764085.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHA25_002855"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHA25_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          955 a          760 c          546 g         1173 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt ttta
//
LOCUS       DAYYFD010000165         3162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000165 DAYYFD010000000
VERSION     DAYYFD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.171"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHA25_002856"
     CDS             complement(63..2555)
                     /locus_tag="ACHA25_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8764086.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHA25_002857"
     CDS             complement(2562..3029)
                     /locus_tag="ACHA25_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8764087.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAYYFD010000166         3021 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000166 DAYYFD010000000
VERSION     DAYYFD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.172"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACHA25_002858"
     CDS             complement(287..649)
                     /locus_tag="ACHA25_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764088.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACHA25_002859"
     CDS             complement(723..1103)
                     /locus_tag="ACHA25_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8764089.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACHA25_002860"
     CDS             1185..2360
                     /locus_tag="ACHA25_002860"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8764090.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..>3021)
                     /locus_tag="ACHA25_002861"
     CDS             complement(2437..>3021)
                     /locus_tag="ACHA25_002861"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8764091.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          941 a          578 c          498 g         1004 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca t
//
LOCUS       DAYYFD010000167         2753 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000167 DAYYFD010000000
VERSION     DAYYFD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.173"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..746
                     /locus_tag="ACHA25_002862"
     CDS             240..746
                     /locus_tag="ACHA25_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8764092.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            763..1662
                     /locus_tag="ACHA25_002863"
     CDS             763..1662
                     /locus_tag="ACHA25_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8764093.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1749..2633
                     /locus_tag="ACHA25_002864"
     CDS             1749..2633
                     /locus_tag="ACHA25_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8764094.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT         1001 a          508 c          535 g          709 t
ORIGIN      
        1 tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
       61 aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa cacaaaatat tatagaacct
      121 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
      181 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      241 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      301 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      361 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      421 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      481 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      541 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      601 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      661 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      721 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      781 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      841 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      901 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      961 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
     1021 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
     1081 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1141 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1201 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1261 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1321 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1381 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1441 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1501 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1561 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1621 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1681 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1741 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1801 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1861 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1921 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1981 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     2041 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     2101 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2161 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2221 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2281 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2341 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2401 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2461 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2521 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2581 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2641 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2701 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat ttt
//
LOCUS       DAYYFD010000168         2675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000168 DAYYFD010000000
VERSION     DAYYFD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.174"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACHA25_002865"
     CDS             699..785
                     /locus_tag="ACHA25_002865"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8764095.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACHA25_002866"
     CDS             complement(1087..1311)
                     /locus_tag="ACHA25_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764096.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACHA25_002867"
     CDS             1677..1961
                     /locus_tag="ACHA25_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764097.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACHA25_002868"
     CDS             1964..2380
                     /locus_tag="ACHA25_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764098.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2675)
                     /locus_tag="ACHA25_002869"
     CDS             complement(2517..>2675)
                     /locus_tag="ACHA25_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8764099.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DAYYFD010000169         2661 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000169 DAYYFD010000000
VERSION     DAYYFD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.175"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(193..759)
                     /locus_tag="ACHA25_002870"
     CDS             complement(193..759)
                     /locus_tag="ACHA25_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8764100.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(759..1715)
                     /locus_tag="ACHA25_002871"
     CDS             complement(759..1715)
                     /locus_tag="ACHA25_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8764101.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1715..2371)
                     /locus_tag="ACHA25_002872"
     CDS             complement(1715..2371)
                     /locus_tag="ACHA25_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8764102.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          828 a          543 c          489 g          801 t
ORIGIN      
        1 aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa aacagcttct catgtttttg
       61 atttggttgg acttattacc gcagatataa gcttttgagc accgtgaata aagaggttat
      121 gaaatcaacg ctctaacctg agtattagag aaaaaaacgc aaaacatatc atttatgttt
      181 tgcgtttttt atttatcttt ttttctcaat acaatataaa ataggcggct ggttcttttg
      241 gttgataaac cgataattca gcacattata tgtttcctgt ggcagtttgg aacaaaaaga
      301 atcaaccata tccagttctt tttctccacc ttcatgacca tagtagacga caaggatcaa
      361 tcgtccgcga ggcacaagtc gtttcaatat ttcttccata gctgtgcgtg tagtctctgg
      421 catagtgatg attgctttat cactttttgg gagataccct agattgaaaa tccctgcttt
      481 gagattttgt tgttcagcta tcacttctcc taacgtttcg tggccttgta gaaacaatga
      541 gacacgttct tctaactgac gctgttctag cctttcacga gtagcatcga ttgcttgttg
      601 ctggatatca aaactataga catgaccagt ttttccgatg tgttctgcta gaaaaaccgt
      661 atcgtggcca ttgcccatcg ttgcatctac gacgtgatcc cctggttgca agatttcttg
      721 cagtaatgta tggctgaaat gcaaagcagt ttgcaacatc agacgctcac ctttttccgt
      781 acatcatatt ttccttggaa gctatctctg cgaagaagtt cttcatcaat tgcattcaag
      841 acttcccatt ttttcaagct ccacattggt ccaatcaagg aatcccacgg cgcatcccct
      901 gtcaaacgat ggatgatgat ctcttttggt atcatttcca gctggtcgca aatgacattg
      961 acatagtcag gacggctcat taattgaagg cgaccttcat gatagtcccg caacattctc
     1021 gttttgcgca tcagatgcat caaatggagc ttgattcctt ggatatctga atctaaaatg
     1081 gtgcgtctga cattttcttt catcatttct aaactttcgc ctggtaatcc gttgatcaaa
     1141 tgcgtacaaa cacggatatt atgtttgcgt aatttagcca cgccatctaa ataagtttga
     1201 taatcatgtg ctcggttgat cagttttgaa gtttcttcaa aagttgtttg caatccaagt
     1261 tctacccata aatacatccg ctcgttcagt tcagcaaggt aatcaactac ttcatctggc
     1321 agacaatccg gtcgtgtacc gactgataat cctacgacac ctggtaaatt gactacttgc
     1381 tcaaatcgtt ctttgattat ttcaactggc gcatgggtgt ttgtaaaatt ttggaaataa
     1441 acgatgtatt gttggacatg aggccatttt tgatgcatca tgtcgatttc tttctggaat
     1501 tggattggaa gagggtcttc aggtgcaacg atcatgtctc ccgaaccaga tacactgcaa
     1561 aaagtacaac cgccatgggc aacagtccca tctcgattcg gacagtcaaa tcctccatcg
     1621 atcggcacct tgaaaatttt cccgccaaac tcatttcgta aagcgtaatt ccaagaatga
     1681 tatcttttat ttccttctga ataaggaaaa gtcattaaaa gtccttcttt ctgtaagagt
     1741 tatctatttg ttttcttttt tctctataca ctggataagt caggcacagc caagaaagac
     1801 tgagtaagta accgccgatg atatcagtag gaaaatgaac gcccagataa atgcggctta
     1861 tcccgacact tactataata aacatcatca gcaattgaag caatgtgcgt aaggttcttt
     1921 tttgaacaaa agctggaagc agaaatagta aagaaccgta aagaatcata ctgcctgctg
     1981 catgtccact agggaaactg taacttgttt caactactaa gtgcgccaat gaaggacgct
     2041 ctcgcatgaa aaatagtttg atcaccgggt tgatgattcc agcaattaca ataaatccag
     2101 aaacgaacca aaatgtttct gccctttttt tcttatacca catccataaa gccgcacaag
     2161 caaataaaat gatgacggta agaggattac cgaatttagt cataaagagt tggtagctgt
     2221 tcagatcagg ataagaagct ctgacaatgc ctgtaaacca ctgatcgaaa ccagaaagcc
     2281 acgacagatg gaactttacg atccagccca aaatgaaaaa caggacaaga aagaagctac
     2341 cagccacctg aaatgatgtt ttatatttca tacaattcct cctttgaagc aatgacttat
     2401 tatagcattt ttggtccagc gaaaaaaacg gtaaagagat aaaaccaatg tatgattcct
     2461 ctttgaataa ccaatattct tataaaaaaa ataactgtaa tcggaaagac attttttggt
     2521 aatgcaaatg aaatgttagg gaaataaaat cagtgttttt cgaaatcctt ttaaacaaga
     2581 acgtagacgt aggaagaact tgaaaatgaa cagaagttgt tcattacggt aaattacaat
     2641 atgattacaa aaaacaacaa a
//
LOCUS       DAYYFD010000170         2585 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000170 DAYYFD010000000
VERSION     DAYYFD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.176"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..422
                     /locus_tag="ACHA25_002873"
     CDS             210..422
                     /locus_tag="ACHA25_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764103.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(700..2499)
                     /locus_tag="ACHA25_002874"
     CDS             complement(700..2499)
                     /locus_tag="ACHA25_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8764104.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          940 a          459 c          471 g          715 t
ORIGIN      
        1 aagaaaaaaa cgcacgaaca acataggaat ataaacaaac gtttttgtca caacgtctaa
       61 gatcgtgtta aaaaatagtt atctaaaaag cagaaagata ctcaaaataa aaaataaaaa
      121 aatgaataaa aacgttttca aatacaaggt aatttgttat tataaaataa agaaaaaaca
      181 aaataaaaat aaagaaaaga gggaatgaaa tggtaaaagc agaagtagga agatcttata
      241 tatgcaaacc gatcggagta tctcatgaac taatcggctg tattgaacgt acatatatca
      301 acacggctgt cgtgtatgtt gaaagttatg accaacgaga ctatcaactt atcaatgaaa
      361 gcaagtatcg catgctcgta aaattctgtg atattagcgc acctactgaa ttggtttctt
      421 aaacagtatt tttgagaatt cgcaagaact cccttataat ataaataaag acagaaggga
      481 gcgagaagat atgccaaaaa gaaaaagttc agggaaaatg ggaattattc ttgcagtgat
      541 ctctgcaatt cctgctattt tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta
      601 agcacacatt atgagcgtca aacaaaaaac gcatatacct gcggtggcag gttatgcgtt
      661 ttttgttgta taaagacttt tttcattaac gatttttgat tagccgactc ccaaaacaaa
      721 atttagcagt ttatctgaat aagtgatttc ttcatctgtt tgtttcacac tttcatttag
      781 tagtcccatc tcatcggtta tgataccatc cacaccataa aacatcatgc gactcatggc
      841 atctgaatcg ttgattgtcc atacatatac ttcttttccg tcttggtggg cagaattaat
      901 gaagttacgg ttgatcgtac tatattccat tgtcagaaaa tcagcaggcg tcacaggggg
      961 cccaacaata ttgaaaggta agatgtagcc cacataaaag tttggttctg cttcttttaa
     1021 atctgaaacg gtttggtacg tcaaactttg caaaatatga tggttctcta agataacttt
     1081 gcggtattta tggacaaatc gttcgacaag gtcatccgaa tcttttttag aggtcttgat
     1141 ctcaataagt aatttttggt ttagcttatg tgctttttcc aaataagaat caaaagaaca
     1201 aacaggggct tgcactccgt tttcagtaac tgtaagattt gtcaattctt tcaatgtaag
     1261 atcctgtggt gttccattta cccctgttaa gtgacgaaga tttgcatcat gcatcactac
     1321 gaaatgacca tcttttgttt cctgaacatc catctctaca taatcaggat gatagtctct
     1381 gcttgttttt tcaagtgctt ctaaagtatt ttgcgcccca ttttgggtac tcacacctct
     1441 gtgagagaca gtcactggtt ttgtctgaac agctgaagtc agataatcca tattgtaaag
     1501 acagacgcca ataccgaaaa aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga
     1561 gaaacgcaag ttgggggctt gtgggataaa ccacttaggg atctcaggca agaagccttc
     1621 gtcatccatg aaatcaacaa tgatataaaa aatccctacg gtagacatca cgatattgaa
     1681 taataaaatc ccttgtaaaa gcgtcatagc aaacacagcg ctgatcaatg catagtctgg
     1741 gaaaaattgt tcaaccatac tttgtgccaa aataacaacg atatatccta aagaagaaag
     1801 gagtaaaatc gtaccgccaa tcacaataaa ttggcccgtg atggccaaaa gacgcgattt
     1861 ggtcaatgac cagctttccc gaatggctgc tcgaaaagaa cgatctcgta gaatcatttc
     1921 cggtaaagca aaaatcagtc gaatcccaat atagccaagg aaaatataaa cgagtaaaaa
     1981 ggaactgacg atgatccaac gattagcaaa aataaaatcc ataatgaaag cggggatttt
     2041 tatttttgac agtagatcag aattaaaact taatccgctg atcggtagaa tcaataaaaa
     2101 ataagcaagg aaaaacaaaa aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa
     2161 gtgtaagagt tgcttcaaag agattggttc tttcttttta ataaaaaaaa cactcatcaa
     2221 aagaaaagta aattcgaaat aaactaacag taaaatcgtc atcaaaacta cgattaacga
     2281 gagtagaacc ccaggatgtt gagaaaaaat tgaaggaata ttgtcataag acaaatagtc
     2341 aattgcccct cggtgtaaaa taaaacgggt cgtactgctc aataagggta aacaaataaa
     2401 taaaataaag ccatgcatca gcaagacatc acggaaataa gcttgtgttc cttttaaaaa
     2461 gtcccaagca ttacctaagc tacgtttcaa ataattcatc ctatcacctg ctcttcacat
     2521 tgtagcgcaa atctaatgaa aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag
     2581 attta
//
LOCUS       DAYYFD010000171         2582 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000171 DAYYFD010000000
VERSION     DAYYFD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.177"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..2513
                     /locus_tag="ACHA25_002875"
     CDS             72..2513
                     /locus_tag="ACHA25_002875"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8764105.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          860 a          460 c          610 g          652 t
ORIGIN      
        1 aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata tttttgtagg
       61 aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg ataggaaagt
      121 atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg actaaaggat
      181 tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt ggaaatgtcc
      241 tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc ggtctccctg
      301 tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa gcagtgatct
      361 tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc ggaacagaga
      421 gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa tatggtgaac
      481 caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca catatgggat
      541 taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag gatcgctatg
      601 ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga
      661 tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca gttcgtggaa
      721 atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa
      781 cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag
      841 aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta gcggaggtcg
      901 gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa ttgacagata
      961 gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca tttgcagctt
     1021 cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat atatatggag
     1081 gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg actacattag
     1141 cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt attggtggag
     1201 gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag gatgtgcaaa
     1261 aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata gaaaaaaatg
     1321 tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa gagctctccg
     1381 atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc gcgcagaatt
     1441 ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca tctgtcattg
     1501 cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc
     1561 tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg atcaatgcag
     1621 ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct tcaatcgtga
     1681 aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt ttccatgcat
     1741 acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac atgatcaact
     1801 cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa gagattctat
     1861 tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt gaaatcccat
     1921 tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg
     1981 caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa gggatcatga
     2041 atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt agcgcttcga
     2101 tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg cagatcaaag
     2161 gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta ggcggagctt
     2221 ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat tcagccaagg
     2281 aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat
     2341 tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca ttagcgatct
     2401 caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa
     2461 agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca
     2521 atatctttag aagaataaag caagatttac agttatttta tttactgcag atcttgcttt
     2581 tt
//
LOCUS       DAYYFD010000172         2579 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000172 DAYYFD010000000
VERSION     DAYYFD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.178"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2258
                     /locus_tag="ACHA25_002876"
     CDS             <1..2258
                     /locus_tag="ACHA25_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8764106.1"
                     /translation="DKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYFNS
                     NEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIVEQ
                     LDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSVLA
                     KDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLETF
                     ELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQSI
                     VLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIELL
                     ISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIFEI
                     DYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAEHY
                     RQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPELSV
                     SITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDILI
                     FVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRNSA
                     NDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPENGN
                     MYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQASSY
                     LFKFKQKRSDG"
     gene            2393..2521
                     /locus_tag="ACHA25_002877"
     CDS             2393..2521
                     /locus_tag="ACHA25_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8764107.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          985 a          339 c          495 g          760 t
ORIGIN      
        1 tagacaaatt agatacgaaa gacattgtca attggctcaa tcaagacgaa gaattgacag
       61 atttattatc ttcggtaaaa aaagaatcaa gtgcaagtca gcaatcgaac tggagtggat
      121 tgtactttaa ttcaaatgag tatgtgaaat acattttgaa taaaactgca tggattaaga
      181 ttgatggaga aaaatatagc ccgagacaaa ttgtgctata tgaaaagctc caggcacacg
      241 ttccgggatt gtatggagtt tctgaacaaa gcttaatgga actcttgggg caacgcattg
      301 ttgaacagtt ggatttcaga aaaaatatgg cgatgttttc ggatgatgaa ataagagcta
      361 tattagttga gttaccaaaa ttcgataaag gcgaaataag tcgcagatta tataatgaac
      421 ttataagaaa taaaaaagga atgaatccta cttattctac cgaaggatta tcagtcttag
      481 ccaaagatgg cttcttttat ccaaacaaag aattaaggta tgctgataag cgactgccta
      541 aatcaataga aaaacaaatg cgttttattt acgtgcctga aaaagctagt acaactacga
      601 tatatgaatg gcttggggtt gaacgcttca aaacaaattt aaaattggaa acatttgagt
      661 tattggaaga ttattctgag gatttcaaaa aagagattga tgatatcaaa actgcggtat
      721 tatctacgat agatgacaat agtaagaatg ttagttcatt aaaacgtatt cagattattc
      781 catgcagcaa aatatctgct aaagatgttg agcaagatgg tcagtcaatc gttttggaag
      841 attattattt tgtggaaagt aaaggaaaat tctacttaaa attacctgct aattctattg
      901 aaattaatca attacgtttg attgatacgt tttcttcaac aatcgtagac atattcaagc
      961 aggtgttaaa tctaagctta gatttgaatt tgattgaatt gttaatttcc cgagatacgg
     1021 aacataaaca aagaaaaata actgaagaat tcggtgtaga taagtggaat gaatcgtttg
     1081 agctattgtt tagcgaaaat gcgttaaata aaaaagttaa tgactttttg ttatcaaatg
     1141 gactaaatga agaggaatat cctaccattt ttgaaattga ctattcatat gcattaaaag
     1201 aagaagagta tcaattgttg gcgatagctc tagaaagcat ttcaaaagat attgaggatt
     1261 tgaatggatt gtcagaattg attcatttgg atattcgtga attttggaaa ctgaaattaa
     1321 gatcttatct agataagaaa gcggaacatt accgccagat tttatatagc aatgcaaata
     1381 aaagcggtga tgaaatactc aaagaaacat tcctagaaaa aattgataga tacaaagatt
     1441 atcaatttga gatttctgaa attccaaatt cagttcaagt cgacattgag aaagaaagta
     1501 agaagagatt tccagagcta agtgtgtcaa ttactgatga aattgacatt gatggcctgt
     1561 atgataataa tgtcgaagag ttagtcacgg ttgcagctat tacaatagat gattttgatt
     1621 attatattag aggtcatcaa aaagtttctt ccttactgta ttttggtatt ccgtcccgtt
     1681 taagagaaga tatactgata ttcgttacta aagaaaatga tgttgatttg aacggagaaa
     1741 aatctggtat ctctattgaa ggaacatcaa ctgttcaaac cattcttgaa ccatcgaata
     1801 gtgttagtag gaaaggtaag aacacacgaa atggagaaag aagtaaacga gattatgata
     1861 atagaaactc cgcaaatgat gatgctggtg aaacagcaga acaaattgct tacgctgaat
     1921 tgactcagaa tcaaaaactt aatgtaattt ggaatacgaa atattctagt aaatcttcag
     1981 atcgtaataa gcagccacca aacggaattg tttgtgatat gtgggttcat gacccagaga
     2041 atgggaatat gtactttgag gtaaaatcat caattacaga atttgaaatg tcgattaatg
     2101 aatataattc tatgttagat aaccctgact cttacgaggt cgttttagtc aatcgtgata
     2161 ccaaagagat aagtcgtcat aaatttgatg aacttgatgg attgaaacag gcaagtagct
     2221 atcttttcaa atttaagcaa aagagaagtg acggttgagt gatttgtttc cacaaaccta
     2281 ttcaatctat caactcaaaa atcataaaca accattatta tagtaggaag atacagagtg
     2341 tactctcaac gcatatcgaa ttagtcgctt cattcgttaa aggagaataa aaatggacca
     2401 aaaagaaaaa atgaaagaat tgatcaaaaa gaaacaaggt ggaggccgat ccaaacaaat
     2461 ggaaacacct aagaatgatc gtaaaaacat gagaaaaggc cctaaaatct ttaataaata
     2521 gacgtgctaa agaaagacta agaagtgtta acttttgtta ttgtttctta gtcttttta
//
LOCUS       DAYYFD010000173         2572 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000173 DAYYFD010000000
VERSION     DAYYFD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.179"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACHA25_002878"
     CDS             complement(45..1364)
                     /locus_tag="ACHA25_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8764108.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACHA25_002879"
     CDS             complement(1361..2014)
                     /locus_tag="ACHA25_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8764109.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2572
                     /locus_tag="ACHA25_002880"
     CDS             2302..>2572
                     /locus_tag="ACHA25_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8764110.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCG"
BASE COUNT          852 a          401 c          248 g         1071 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgt attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gc
//
LOCUS       DAYYFD010000174         2564 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000174 DAYYFD010000000
VERSION     DAYYFD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.180"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..1614)
                     /locus_tag="ACHA25_002881"
     CDS             complement(100..1614)
                     /locus_tag="ACHA25_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8764111.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            1752..2558
                     /locus_tag="ACHA25_002882"
     CDS             1752..2558
                     /locus_tag="ACHA25_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764112.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT          687 a          553 c          421 g          903 t
ORIGIN      
        1 ctttttcaca acctcttcac taaacagtgt tcaatcagct gaccctcggt attagctcaa
       61 aacgctgatt tcacaacctc tttttattta cgttctattt cattcatttt ccgaagcaaa
      121 tcgagctgct tgtatgcccg cttgtcgccc aaaaataatg atctccgcaa ctgaatttcc
      181 accgatccgg ttgtttccat gtaagccacc tgtgacttca ccagcagcat ataaccccct
      241 aattggctgg ttttctttat ctaatacttc agtgttcgta ttgattttca caccgcccat
      301 cgtgtaatgg attcctggtg cgattttgat cgcataataa ttcggagacg ataagtcatg
      361 ttccatggct gtggcacggt tgaattgagc atcttttttc tccgcaacat ccgtattcca
      421 tccatcaact gtttttgcta gtgtttcttt tggtaaatcg attttttctg ctaattcttc
      481 cattgtcttt ccagttgtga catatccttt ttggtcataa aattcaatag ctgtggcacg
      541 atcacgcacg ccttggtcaa aaatcaaata agctgatttt tcaggcaatg ctgtaattgc
      601 tgcagatact ttatcccttg tatccatttc attgacaaat cttttcccct cttgatcgac
      661 caagattgct ccttctccgc gaacaacctc accaatcaag accccctcat cctgctgcac
      721 agttggatga atttgtattt tatccatatc aacggtttgt ccacctaatt tttcaatcat
      781 ggtgatacca tctccagttg atccttcttg atttgtcgta atataatcag ctaaatcggg
      841 acgatatttt tcaataaatt ctttgctggc accaaaaccg cctgtggtaa ctacgattgc
      901 ttttccggtg acctcttttg gttcttctcc ttgaacatgt acagtcactt cattgaccgt
      961 ttccccattt ttcttaatgt ctgtcacatc tgcgttgaca aaaataggga tttcccgctc
     1021 atggacgtta ttcaataacc cctccactaa gtatccacca atagccgagc catcagacgg
     1081 acgatgtgtc ctcttttcac tcatccctcc agtgatcgtt aagttgtcca gtttgatacc
     1141 gttttgatct agccaatcga ccgcatcagc agagtgatcg acaaagtatc gaagcatttc
     1201 tttatcattc gttcctccac caccttttag cgtttcttca tagaaggcat cattcgagtc
     1261 tttgattcct tcttcttttt gaaattttgt ttctgaagca ttcataccgc ttgatgattt
     1321 agatgtgttt cctccggcaa caggcatctt ttccaaaatt actggattca acccagcatc
     1381 ttttgcttcg atagctgctg ccatccctgc accaccagct ccaataatca cgacatcata
     1441 agattctttc atttcttttg gatctgtata tacttgttca gaagcgcctg atgatacttc
     1501 ggcagattcc ttcgtactag aacttgtctg cgctgtattt ttctgatctg cgccgcatgc
     1561 gctcatagca agaagtgcaa cggctgttaa taaaccagca actaattttt tcatttgtat
     1621 cccccttttc tccttatact atattaagtg atttttttca caatatcaac tctgtttttc
     1681 tttaagtcat atagctttgg taaacacacg cttcttagta taattaagga tatacatgaa
     1741 ggagttattt tatgaatcat ttgaaaacct tttttacgaa cagtacaaaa actgcaggta
     1801 gaatcacaaa cggaatctat attttattac gtatcatcgt cgttattatg ctagtggatc
     1861 aggtctggaa ccaacactgg ggaaatacaa tgcttttatt actgacatta attttatttg
     1921 aaatccctta ttttctggaa aagatattaa agatacagat tccaaatatg ctagaaatca
     1981 ttttgttatc ttttattttt agttcaacag ttttaggaga gttaagtgat ttttatggct
     2041 atttttcaat gtgggatact tttctacatg cattcagcgg atttttagct gggggtgtcg
     2101 gcttttccct tgtttatttg ctcaataaaa atacaaaaac catgaacttg actcctttat
     2161 tactagcagt tgtttccttc tgtttttcga tgacagttgg tgttatgtgg gagtttgtgg
     2221 aattttcagc agataaatta ctgcaattcg atatgcaaaa agatacgatc atccagcaaa
     2281 tcaatagtgt ggagttaagt aaagacggaa acactgtcta tcatgttcca gatatcactc
     2341 ggacaatcat tgaaagtcaa aatgcagatg gtaaaacaga taaaacaatc atccaaaatg
     2401 gttacttaga cgtgggaatc atggatacta tgaaggattt atttgttaat tttattggag
     2461 cagttgtttt cagtgtattt ggttacctct atagtcgaaa cgattaccaa aaatttacgt
     2521 tcatcaagaa ttttattcct aaaaaaatca aacgataaaa catg
//
LOCUS       DAYYFD010000175         2551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000175 DAYYFD010000000
VERSION     DAYYFD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.181"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHA25_002883"
     CDS             207..2069
                     /locus_tag="ACHA25_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8764113.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="ACHA25_002884"
     CDS             2069..2401
                     /locus_tag="ACHA25_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8764114.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          914 a          371 c          494 g          772 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 atatttagtc taatggtgat aagtcaagat gaaatttaag attttcttta ttgagtaata
     2461 tgttttgaag cgcacgaaac tagacccgat caaaatcaaa tttttttcgg gttgattcaa
     2521 ggatttgcgt caataacgga ttgttatttc t
//
LOCUS       DAYYFD010000176         2540 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000176 DAYYFD010000000
VERSION     DAYYFD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.182"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..245)
                     /locus_tag="ACHA25_002885"
     CDS             complement(<1..245)
                     /locus_tag="ACHA25_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764115.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKK"
     gene            407..1516
                     /gene="pdhA"
                     /locus_tag="ACHA25_002886"
     CDS             407..1516
                     /gene="pdhA"
                     /locus_tag="ACHA25_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8764116.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1519..2496
                     /locus_tag="ACHA25_002887"
     CDS             1519..2496
                     /locus_tag="ACHA25_002887"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8764117.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT          846 a          471 c          532 g          691 t
ORIGIN      
        1 tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt
       61 gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca
      121 acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat
      181 gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt
      241 ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca
      301 tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg
      361 cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa
      421 agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca
      481 agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga
      541 tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac
      601 agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag
      661 tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga
      721 tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg
      781 acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat
      841 cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa
      901 aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc
      961 aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta
     1021 tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt
     1081 cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt
     1141 atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact
     1201 gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa
     1261 agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac
     1321 ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga
     1381 aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt
     1441 gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc
     1501 gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta
     1561 gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac
     1621 ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc
     1681 gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga
     1741 tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta
     1801 gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca
     1861 attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa
     1921 ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca
     1981 aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg
     2041 aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat
     2101 aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg
     2161 cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt
     2221 gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact
     2281 ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt
     2341 tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca
     2401 cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat
     2461 atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc
     2521 atggcttatc aatttaaatt
//
LOCUS       DAYYFD010000177         2518 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000177 DAYYFD010000000
VERSION     DAYYFD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.183"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..257
                     /locus_tag="ACHA25_002888"
     CDS             6..257
                     /locus_tag="ACHA25_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8764118.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            359..2356
                     /gene="tkt"
                     /locus_tag="ACHA25_002889"
     CDS             359..2356
                     /gene="tkt"
                     /locus_tag="ACHA25_002889"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8764119.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
BASE COUNT          838 a          465 c          568 g          647 t
ORIGIN      
        1 tgagcatgct gtcacctgaa aaaatagagc ggatcaatga acttgcacgt aaaaagaagg
       61 aaaatggtct ctcaaaagcc gaagaacaag aacaaatcaa cttgcgcaaa gagtatttgg
      121 aagcttttcg ttccggtatg cgtcatcata tcgaaggaat gaaagtcgtc gatcctgagg
      181 gcaatgacgt gaccccggaa aaattaaaag aaatccaaaa gaaaaaaggc ttacacaacc
      241 gaaatgacaa cttttagtaa aaaatactga aaaagattgc tttttcgtgt gacatcttat
      301 agaatgaaag tgtaagaaat aaaggtaaaa aaaacctaaa ttaataggag gggttatttt
      361 gtttgacaaa attgaccaac ttggcgttaa cacaattcgt acattaagta ttgaagcagt
      421 ccaaaaagca aactcaggac atcctggatt gccgatgggc gcagcaccta tggcatatgc
      481 gttatggaca aaacacctga aagtaaatcc aacgacatca aaaaactggg ctgaccgcga
      541 ccgtttcgtt ctttcggcag gacatgggtc agcaatgttg tatagcttgc ttcatttagc
      601 tggctatcaa gtgacaatcg atgatttgaa acaattccgt caatgggatt ctaaaacacc
      661 aggacatcct gaagtacatc atacagatgg tgtagaagcc actacagggc cattaggaca
      721 agggattgca atggctgtcg gtatggcaat ggcagaagcg caccttgcag caacgtataa
      781 caaagaaaac tttaatgtga tggatcacta tacgtatgca atatgcggtg acggagactt
      841 gatggaaggg gtatctcaag aagcaagttc gatggcaggt catatgaaac tagggaaatt
      901 gatcgtttta tacgattcaa acgatatttc actagacggt ccaacttcaa aagctttcac
      961 agaaaacgtt ggtgctcgct atgaagcata tggctggcaa catatcttag tgaaagacgg
     1021 aaatgaccta gaagcaatct ctaaagcgat tgaagaagca aaagctgaaa cagacaaacc
     1081 aacattgatc gaagtaaaaa cagtcatcgg ctatggtgct cctaaagaag gaacatctgc
     1141 tgttcatggt gctcctcttg gtgaagatgg catcaagatg gctaaagaag tttatggttg
     1201 ggagtatcca gatttcactg ttccagaaga agtagctgcc cgcttccatc aaacgatgat
     1261 agaagaagga caaaaagctg aagatgcatg gaatgaaatg tttgccaatt ataaaaaagc
     1321 ttatcctgaa ttagcacaac aatttgaaga tgcttttgat ggcaaactac cagaaaactg
     1381 ggatgcagaa ttgccaactt atgaagttgg aagcagtcaa gcaagccgtg tttcaagtaa
     1441 agaagtcatc caagaattat cgaaagctat accaagcttc tggggtggtt cagcggactt
     1501 gtcaggttcc aataatacaa tggtggctgc agataaagat ttcactcctg aacattatga
     1561 aggacgcaac atctggttcg gtgtccgcga atttgcaatg gcttcggcta tgaatggtat
     1621 ccaacttcat ggcggaactc gtatctacgg tggaacattc tttgtctttg tcgattatct
     1681 aagaccagca gttcgtttag cagcaatcca acatactcct gttgtttacg tcttaacaca
     1741 tgactcggtt gctgtcggag aagacggacc aacgcacgaa ccgattgaac aactagcatc
     1801 tgttcgctgc atgcctggtg ttcaagtgat tcgtccggca gacggaaatg aaacacgtgc
     1861 tgcttggaaa gtagcgatgg agacaacaga tgcgcctact atccttgtat tgagtcgtca
     1921 aaaccttcca gtattgcctt ctacaaaaga agtagcagat gaaatggtga aaaaaggcgc
     1981 gtatgttctt tctccatcac aaggagaaac accagaaggt attttgattg caacaggttc
     2041 tgaagttgat ttggctgtga aagctcaaaa agaattggct gaaaaaggca aagatgtttc
     2101 tgttgtttct atgccaagct ttgacctctt tgaaaaacaa tcttctgaat ataaagaaag
     2161 tgtacttcct aaatcagtga agaaacgtgt ggctatcgaa gctgctgcat catttggatg
     2221 ggaacgctac gtaggaacag agggcgcaac gattacaatc gatcatttcg gtgcatctgc
     2281 ccctggcact aaaattttag aagaatttgg atttacagta gaaaatgtcg tgaatacgta
     2341 caatcaacta tcatagatcc aagcaattgt ttgactgata agaacagctg tctgcaagtt
     2401 ggagacagct gttctttatg tgttagaaga ataaatcaat attacttttc gttttcggct
     2461 atgttacatt taaatgacaa aacaaagaat tcttcggatt agtttgtgtt tcttgaga
//
LOCUS       DAYYFD010000178         2426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000178 DAYYFD010000000
VERSION     DAYYFD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.184"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..2276
                     /locus_tag="ACHA25_002890"
     CDS             123..2276
                     /locus_tag="ACHA25_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8764120.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          829 a          426 c          536 g          635 t
ORIGIN      
        1 caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct
       61 tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg
      121 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
      181 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      241 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      301 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      361 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      421 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      481 ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag
      541 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      601 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      661 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      721 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      781 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      841 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      901 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      961 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
     1021 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
     1081 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1141 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1201 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1261 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1321 ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1381 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1441 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1501 catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1561 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1621 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1681 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1741 aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1801 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1861 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1921 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1981 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat
     2041 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     2101 gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2161 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact
     2221 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2281 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2341 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2401 cggcaaaaaa ggttgtgaca aaaatt
//
LOCUS       DAYYFD010000179         2395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000179 DAYYFD010000000
VERSION     DAYYFD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.185"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHA25_002891"
     CDS             316..570
                     /locus_tag="ACHA25_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8764121.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHA25_002892"
     CDS             571..888
                     /locus_tag="ACHA25_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8764122.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHA25_002893"
     CDS             1447..2382
                     /locus_tag="ACHA25_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764123.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAYYFD010000180         2275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000180 DAYYFD010000000
VERSION     DAYYFD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.186"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..526
                     /locus_tag="ACHA25_002894"
     CDS             8..526
                     /locus_tag="ACHA25_002894"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8764124.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            654..1337
                     /locus_tag="ACHA25_002895"
     CDS             654..1337
                     /locus_tag="ACHA25_002895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8764125.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1646..1719
                     /locus_tag="ACHA25_002896"
     tRNA            1646..1719
                     /locus_tag="ACHA25_002896"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1680..1682,aa:Arg,seq:tct)
     gene            1878..2237
                     /locus_tag="ACHA25_002897"
     CDS             1878..2237
                     /locus_tag="ACHA25_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8764126.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          762 a          353 c          456 g          704 t
ORIGIN      
        1 gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt tgattatatt
       61 tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat
      121 tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc
      181 gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa
      241 aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttatacaaa
      301 agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata ttatcggtag
      361 tcccatcttt cagattccat atattggact agtcctttca aaaacggcat ttgcatggat
      421 tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat ggaatacctt
      481 tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat gttaaaaaca
      541 taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa aacactcatg
      601 cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt agtatgcgca
      661 atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg cgtacccagc
      721 aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta gcagaagtac
      781 cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag cttcagcctg
      841 aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg gccacgacaa
      901 tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc gagaaagcta
      961 aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa ggtcaagcag
     1021 cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat agtgcaaaat
     1081 caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt ggcgttttga
     1141 tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag attccccgtg
     1201 gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg gatcatcccg
     1261 atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca aaggaatcag
     1321 aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt gaatcgaggg
     1381 ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag gatacaggaa
     1441 atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat tgataacctt
     1501 agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg ggccaaaata
     1561 attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt aattatctat
     1621 aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag catccgcctt
     1681 ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg ttagaaactg
     1741 aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc aaaggcaaat
     1801 aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata aaactcatca
     1861 gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta gttgcaattg
     1921 aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg acaagtaggg
     1981 tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg tttaagaacc
     2041 aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat ctttcttccg
     2101 catctaaaga aatcacaagt atgctattaa tatctatttt ttttgttgca ttatatggca
     2161 gtttgactag cgatcgttgg attttactca aacaaggtgg gctagctctg ttggcattgc
     2221 tttctatttt cttataaata tagaaaaaat aagaatttat ctaacaggaa acttt
//
LOCUS       DAYYFD010000181         2192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000181 DAYYFD010000000
VERSION     DAYYFD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.187"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..540
                     /locus_tag="ACHA25_002898"
     CDS             91..540
                     /locus_tag="ACHA25_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764127.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..1034
                     /locus_tag="ACHA25_002899"
     CDS             540..1034
                     /locus_tag="ACHA25_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764128.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1048..1479
                     /locus_tag="ACHA25_002900"
     CDS             1048..1479
                     /locus_tag="ACHA25_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764129.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            1481..1618
                     /locus_tag="ACHA25_002901"
     CDS             1481..1618
                     /locus_tag="ACHA25_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8764130.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1655..1948
                     /locus_tag="ACHA25_002902"
     CDS             1655..1948
                     /locus_tag="ACHA25_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764131.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1945..2169
                     /locus_tag="ACHA25_002903"
     CDS             1945..2169
                     /locus_tag="ACHA25_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8764132.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          812 a          329 c          469 g          582 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaattcaa gttatggatc agcttattca tttgttatca
     1021 cttattcaat ttaagggagg aaatctaatg aaagtagttt acaaatcaat caagccttac
     1081 ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc ctgaaaactg tacagagatt
     1141 aaaccaccag ttcctaactg gagaccaaga tttgattttg ataaaaaaca gtgggttgag
     1201 ttagctactg aagaagagaa aaaagggaat gctgttgata gcgtagatga attggctaat
     1261 attaaggcat tatacgaaac gctgaaggca gaaaatgatg aattgaaaca gctaaattct
     1321 aaagcaatgc taaacaatgt agcaatcaag caagaaaatg tcttattgaa agaaaagtca
     1381 gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt ctgtacaaaa taccaaggaa
     1441 atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac atgtattcat atgaagatat
     1501 caaactaatg tatgactggg gctgtttcac taacgaacaa gtaatggttt tcgttccatt
     1561 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
     1621 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
     1681 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
     1741 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
     1801 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1861 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1921 aactttgttt agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc
     1981 gcactggtaa ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat
     2041 tggcagtaca cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt
     2101 gtgttgggtg tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga
     2161 aacaaatgaa aaagaaaatt actattactg cg
//
LOCUS       DAYYFD010000182         2150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000182 DAYYFD010000000
VERSION     DAYYFD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.188"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACHA25_002904"
     CDS             288..695
                     /locus_tag="ACHA25_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764133.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACHA25_002905"
     CDS             709..1110
                     /locus_tag="ACHA25_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764134.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACHA25_002906"
     CDS             1112..1483
                     /locus_tag="ACHA25_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764135.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="ACHA25_002907"
     CDS             complement(1519..1818)
                     /locus_tag="ACHA25_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8764136.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1824..2054)
                     /locus_tag="ACHA25_002908"
     CDS             complement(1824..2054)
                     /locus_tag="ACHA25_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764137.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          782 a          289 c          354 g          725 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
     1801 tgtctcatta aaaagcataa ccattactcc gatctgtttt taaaattagt ttctacctct
     1861 aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca
     1921 ggttgtttat tttcggaaaa agctaatata gctaagattg aaacatccat ctggaattta
     1981 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
     2041 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
     2101 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt
//
LOCUS       DAYYFD010000183         2146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000183 DAYYFD010000000
VERSION     DAYYFD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.189"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACHA25_002909"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACHA25_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACHA25_002910"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACHA25_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACHA25_002911"
     CDS             1898..2059
                     /locus_tag="ACHA25_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764138.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          775 a          332 c          364 g          675 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaa
//
LOCUS       DAYYFD010000184         2014 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000184 DAYYFD010000000
VERSION     DAYYFD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.190"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..768
                     /locus_tag="ACHA25_002912"
     CDS             <1..768
                     /locus_tag="ACHA25_002912"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8764139.1"
                     /translation="KNEKKLFLNTTFENLTLINPNIMNFEDESIIEASGFRIQPEYEL
                     FKRLFDIIVSLVLLVIASPFMLFTAILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSM
                     TATAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFF
                     VDQFNAENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKIL
                     LQTIKILFDKMSSQGLEEDENVISLAEIAFPNEKIFR"
     gene            784..1824
                     /locus_tag="ACHA25_002913"
     CDS             784..1824
                     /locus_tag="ACHA25_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8764140.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            1843..>2014
                     /locus_tag="ACHA25_002914"
     CDS             1843..>2014
                     /locus_tag="ACHA25_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8764141.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGG"
BASE COUNT          717 a          316 c          381 g          600 t
ORIGIN      
        1 aaaaatgaaa aaaaattgtt tttgaataca acatttgaaa acttgacatt gatcaatcca
       61 aatattatga atttcgaaga tgaaagtatt attgaagcat ctggatttag aatccaacct
      121 gaatatgaac tattcaaacg tttgtttgat atcatcgttt cacttgtttt attagttatt
      181 gcttcaccat ttatgttatt cacagcaatt ttagtcaaag tgacatcacc gggaccaatc
      241 atctataaac aagtgcggat cacaaaaaat caaaaggaat tttcaatcta taaattccgc
      301 tcgatgaccg ccacagccga agcaaaatct ggaccagttt tagctaagag caatgatgca
      361 cgagtaacgc ctgtaggtaa atttattcga gctgtccgct ttgatgaatt accacaaatc
      421 ttcaatgtgc tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg tcctttcttc
      481 gtcgatcagt tcaatgcaga aaacccttat tactacttac ggcataatgt tcgtgctggt
      541 atcactgggt atgcacaagt ctatggaaaa tatgtctctg attacaacag taaattacgt
      601 tttgatttac tttatatcaa aaaatattca ttgatgcttg atttaaagat cttgctgcaa
      661 accatcaaga tcctatttga taaaatgtcc tctcaaggac ttgaagaaga cgaaaatgtg
      721 atctcattgg cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt gggaggcgta
      781 aaaatgaaca atccgaaatt cagcataatc gtaccagtat acaatgcaga agcaactatt
      841 ggtcgaacga ttgatattct aaaagaattg gattatgaag agtacgaagt cgttttagta
      901 aatgatggat caacagatca aacacaggaa atcattcaag aactgattga aaatgatgaa
      961 cgttttgaat tgattactac gaccaatcaa gggcccggat tagcaagaaa cgaaggaata
     1021 agacagtcaa aaggagaata tctcttattc ttcgatgcag atgacacgcc agtaaaaacg
     1081 atcttgtcag attatgatca tttgctagaa aagagtccaa aagcggatct tgttgttagt
     1141 tcgtttgttt ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga aaacttagtt
     1201 acagaatgtc actatctaac tcacgaagag tttataaaag acatgtatca gttaatgaat
     1261 gaacagttga tgtatgttgt atggaacaaa tgctatcgta gcgatattct gaaaaataac
     1321 caaattctat ttaaaactta taatagttgt gaggatcgaa tatttaactt acattattat
     1381 gaatactgtc atcaagtgat catgaaccca aaagtagaat atatttatga atttgaaggt
     1441 gggaagggga tcactaatca ataccgacct aacaagttcg aaacatttaa agaattctat
     1501 tcattagcaa atactgtaac agacgatatt aataaagacg gtatggcggc gcttctttta
     1561 aaagggacaa cttcagttat tttttctatt tatagtacct ctgcaagaac ttcaaaagag
     1621 aagaaagacg aagcaaagca gatattgagt gatccagtga tccgagaagc caagcaaatt
     1681 gctaagacgg attcttcagc aaaaaaaata acaaagttgc tttacaatat gccagctcca
     1741 ctatttttat ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc agggttaatg
     1801 gctgctttga agcgagtata ttagttaatg gaggaaaata taatgaaaag aattttgcat
     1861 tttcagggac gaatgggttt aggtggagca gagtctttca tgatgaattt atatcgtaaa
     1921 attgatcgta caaactatca atttgatttt ttgatttatg aagattatgc agatgttcaa
     1981 gactatcatt cagagattga aagattagga ggcc
//
LOCUS       DAYYFD010000185         1921 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000185 DAYYFD010000000
VERSION     DAYYFD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.191"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            270..836
                     /gene="amaP"
                     /locus_tag="ACHA25_002915"
     CDS             270..836
                     /gene="amaP"
                     /locus_tag="ACHA25_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8764142.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            849..1037
                     /locus_tag="ACHA25_002916"
     CDS             849..1037
                     /locus_tag="ACHA25_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8764143.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1050..1607
                     /locus_tag="ACHA25_002917"
     CDS             1050..1607
                     /locus_tag="ACHA25_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8764144.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1639..1881
                     /locus_tag="ACHA25_002918"
     CDS             1639..1881
                     /locus_tag="ACHA25_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8764145.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          707 a          296 c          395 g          523 t
ORIGIN      
        1 aaatagaatc ttgtaataat aactgagcat aatctttgca atttttaatc ttgaataata
       61 tcctttaagt atagccagca atcaaaagta aatagagaat aagtaagcaa ttgttatttc
      121 agtatcagac tggcaataaa acgccgttat gatccagcaa tgtttggtag cgctttattt
      181 actgatgcta tgctaactgt tcgctatcta tactgtgatt gtcggtagag ttatttacag
      241 ggtaaaaata agtttatagg aggtggaaaa tgaataaggg gttaaaaata atcggtgtta
      301 tcatgagttt actgctactt tcagtatttc tagcgattgc gttgatcaat gcgccgtata
      361 ttatgcctga acaattggaa cgatttcgct tctttacttt aacgaattac tacatgcaac
      421 aatatatttt ctgggcagct gtagtatttg ccgttcttgc tattatcatc ttgctagtcg
      481 ttcttttcta tccaaagtca agaggaactt tcgttatgaa acgggaagat ggcaaactaa
      541 caatcgacaa aaaagcgatc gaagggttgg tacgttccca tttacacgaa gaagagttca
      601 ttcattcacc taaagtacgg atacgttcaa cgaaaaatcg aatccatatc catgtcaacg
      661 gagacttgaa acgaacttct tcattagtag ggaaaacagg tgtattgatg caagatatcg
      721 aagaagaagt aactaaagta ctcggaacaa aagaaacggt caaagtggca gtcacataca
      781 gcggttatga agaacaagaa gatacaagag actacaagca ctctcgagtg gaataggagg
      841 gccaaataat gaaagaacta ttttcagcat acaagctccc tattatcttt gggggtcttg
      901 gactactgct agctgtttta tggttaacga ttggattttt caagacattg ttgatcataa
      961 tcatgacagc aattggcgca ggaatcggtt tttatttgaa acagtcagga atagttgatc
     1021 aattttttaa atcataggag gaaacaaaga tggaaaacaa aaacatgaat acaacaaatg
     1081 gatcagtttt ggcaaatgaa aaaggagaaa accgcatgat gaatggtgaa ttgacttatg
     1141 aagacaaagt catccaaaaa atcatcggtt atgcattgaa agatgtagat ggtctattga
     1201 cattagatgg cggatttttc tcaaatatgg ctgaaaaatt agtcaacaca gataacgtaa
     1261 cagcaggtat caatacagaa gtaggtaaga aacaagtagc tgtggatcta gatgtgatcg
     1321 tagaatacgg taaagacatc gaagatattt acaaccaaat caaagaattg atcagtactg
     1381 aagtaaacaa tatgacacat ttagatgtta tcgaagtgaa tgtgaatgtt gcagacatca
     1441 aaacacaaga agaataccaa aaagatcaag aaacagtaca agacaaagta acagaagcag
     1501 caaaatccac aggacaattt gcttcacgcc aaacagataa agcaaaatct gctgtaggca
     1561 aaggtgcaca aaaagtcaaa gaaaataacg aaccacgtgt acaataagaa gtaagaagtc
     1621 agttaggagg aatttaaaat gggatttatt tgggcattaa tcgtcggcgg tatcattgga
     1681 gcaattgcag gagcaatcac gaataaagga tcatcaatgg ggatcatcgc aaacatcatt
     1741 gctggtttag taggttcagc aattggacaa gctttactag gaacttgggg accaacaatt
     1801 gcaggaatgg ctatcattcc atcattgatc ggtgcaatca ttttagtatt agtcgtttca
     1861 ttctttgcac gtaaattata agcaaattga agaaaaactt atgttgaaat gaagaacaaa
     1921 t
//
LOCUS       DAYYFD010000186         1918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000186 DAYYFD010000000
VERSION     DAYYFD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.192"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHA25_002919"
     CDS             <1..1087
                     /locus_tag="ACHA25_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8764146.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..>1918)
                     /locus_tag="ACHA25_002920"
     CDS             complement(1305..>1918)
                     /locus_tag="ACHA25_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8764147.1"
                     /translation="PQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVRSEE
                     LLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWK
                     KTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEH
                     NKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          659 a          344 c          334 g          581 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggac
//
LOCUS       DAYYFD010000187         1893 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000187 DAYYFD010000000
VERSION     DAYYFD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.193"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..371)
                     /locus_tag="ACHA25_002921"
     CDS             complement(<1..371)
                     /locus_tag="ACHA25_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8764148.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYS"
     gene            1065..1451
                     /locus_tag="ACHA25_002922"
     CDS             1065..1451
                     /locus_tag="ACHA25_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8764149.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1545..1736
                     /locus_tag="ACHA25_002923"
     CDS             1545..1736
                     /locus_tag="ACHA25_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8764150.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          711 a          281 c          253 g          648 t
ORIGIN      
        1 aaagaataca aagtcaaatt ttgcaatgaa ttatagagtc gttctctatc agccattgca
       61 acattcaaat tgttcatttt tagtttaaaa aaaactttat caatattatt tgctctaaat
      121 ggaccaaata agtaatatct ggttttaaac atgtatagaa gaaacatctc attaaaatca
      181 ccagtaaaga aatggaaatc tttagttttt ttaatattat cagttagtaa ctttttataa
      241 tcataaaaaa aataagtagt tctatctgag cgatactctt ttataatact gaattcggaa
      301 ttaaaaacca cgataggtaa ctttaaacaa acatgaagag actttaataa gacttcttta
      361 tctgaaaaca cttttgtttc tctctttcct ttctacatca ttaaatagta ttttatacgt
      421 tatctaaaat actattcctc tgtcaaaata agctaactta cttcattata aaatatccac
      481 ttatcgcttt catgtatatt ccaccgtaaa aagtacattt tatcaccgag aactcatcat
      541 ttttcatcat gatgaatact atttacatat atctagttca aagacaataa ttattatgat
      601 ccgttattat ctaagcacta tacagataat aggaattttt cgtttctaaa tattccaatt
      661 tgggaatcca ataaaaattt tcgttcaaat acgtgtctat tcgaactctt ttctagttgt
      721 taattatgaa gggggaagaa ataaaaaata caatctatac acgaataaga atattatcca
      781 aaaggaggtt ttagaaaaga tttattatac gtaaaaagct caagacattt tcttagccat
      841 aaggacatgt ttgatattta agaagatata tgaattttct tttctaagta taaagataac
      901 tatccattat caactaaaaa aacatttttg aaaataaaca tcagcagtcc tatcaatgca
      961 tgtgagctta tagaagttat tcgcagcgca gcataattgc attagcttct tttcgtgttt
     1021 tttgcctctt ttccctttaa atcttaatgg aggaggtgtg caatatgtca gataacgacg
     1081 aacgcgtaaa tgcccagttg attcgattct ttgaaaaggt cattttgaac accgctaata
     1141 cttattataa aaaacaagaa gaaattcaaa aacatgaaca acttgatcaa ctcccaccat
     1201 atttgtttac taaagatact ataaaattta aacaaccgat taatgtttta aatgtgactc
     1261 tccttgttga agatataaaa ttagcaaaaa tccttccttt gttgaaagaa cctgaacaaa
     1321 tttttcttat cgaaaaattt atctttgata aaactgataa agaaattgga aaggttctgg
     1381 ggattacaag acagggtgcg acaaatttaa aacatcgact ttacaaaaaa attcaaaaaa
     1441 tgctggttta atatctgcat aataactaat tttgtatgct tctttgctcg ttctcctcct
     1501 tcccaaagaa atagattata agtccatggg aagaaggtag atttatgaat tctttaaaag
     1561 aagatttcat tttagcaaag gctggaaatg aagaagctgt tgaggcaatt ctcaaaagat
     1621 ttagctcttt gattcataag caatcctgga gaactggaaa atatgatcaa gattgttatc
     1681 aagaatgtat gcttgcaata tatctagcta tctcgaaatt cgaaattaaa gaataactca
     1741 actaagggag tctgtgacat aagtaaaaag atagtttcaa atttcgaaca ataaatttga
     1801 aactatcttc tttataatta taaagtatcg attttacagc gttctataat gattcataga
     1861 atctaaaacg aatgatttta attatcaacg ttt
//
LOCUS       DAYYFD010000188         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000188 DAYYFD010000000
VERSION     DAYYFD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.194"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHA25_002924"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHA25_002924"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8764151.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYFD010000189         1787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000189 DAYYFD010000000
VERSION     DAYYFD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.195"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..782
                     /locus_tag="ACHA25_002925"
     CDS             186..782
                     /locus_tag="ACHA25_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8764152.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(840..1322)
                     /locus_tag="ACHA25_002926"
     CDS             complement(840..1322)
                     /locus_tag="ACHA25_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764153.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1428..1625)
                     /locus_tag="ACHA25_002927"
     CDS             complement(1428..1625)
                     /locus_tag="ACHA25_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764154.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          601 a          360 c          274 g          552 t
ORIGIN      
        1 aacacaacaa tgaaagcgat aactaagaaa atccttacat aacgattttt cttattaaaa
       61 ttgttttttg aacttcgctt attttttgtg agcatatctg tttacttact ttttgtaagg
      121 tgctatactt catttcgtaa tcaattaaaa aaatgcaaaa cgcaaatcaa aggagaaatt
      181 ttattatgac aacttttact gacactttga aaaaccgccg ttcaatctat catctaggac
      241 gtaatgtttc actttctaat gaagaattaa caactttgat caaagaagca attaaagaaa
      301 gcccaactgc tttcaatgct caatcaacac gcgcagttat ccttttcgga gatgcacatg
      361 aaaaactttg ggaaatcact gaagaagcac ttcgcccatt gactccagca gaagctttcc
      421 caaatacaca aaacaaatta gctggtttca aaaatggtta tggtacagta ttattcttca
      481 aagatactga cgtggtaaaa ggtctacaag aacaatttga attatatgca gacaacttcc
      541 cagattggtc agaacaatca aacggaatcg caacagctaa tacttgggta gcattagttg
      601 ataaaggatt gggcgcaaac ttgcaacatt acaacccagt tattgacgaa gcagttgcaa
      661 aagaatggaa tatcccttca aactggaaac tacgttcgca attagtattc ggttcaccag
      721 aaacaccggc tgaggaaaaa gaatacatga acgatgccga tcgcttccgc gtatttggat
      781 aattttattt gaaataaaaa acagaggttg tgacaaacat cttgtcgcaa cctctgtttt
      841 tattctggat caatcataat atcaatctct gcctcatcgt ttaagcgttg ggacaaaaat
      901 ggaactaatt cttctaatga tgcagaaaat ttcagcatcg gtcctcgcca tccgtttcgg
      961 aaaaaattca tttttacgat tttagaacct tttggctgat ataatacaat tttattgatc
     1021 tcagaaaaag gaacaccttt tttgtcaaag ctattcaaaa caagtccttc ttctgtgatt
     1081 cctctactgt ccaacaaaaa actaataaaa atcattgctg acaagatccc ttttgccaat
     1141 tcatctgctg taggatgact gatgagtgta taccaaatga ctagtgacac taagaagggt
     1201 atcaccaagt agacccatcg atttcgcaca gtccgataaa taatttcttt tcgaaccagc
     1261 caaagaaata aaaacatcac aacaacgaat aaaaacaata gaaccgtaaa gaatgttacc
     1321 atgacaactc gcctcgcttt ttcttctcca gaaaatagga aaagaatact gatttacctt
     1381 ctcaaagaaa ttggacttgc agcacgccgc acgtccaatt gttttcttta ttcaaatgta
     1441 tcatattttg caacaaccat gcttgctttt tcatctaata attcttggtc ctctgcagca
     1501 cattgggcaa ctttgaccac ggcgcagttt gtcatttttg atacaacttc accaatgacc
     1561 gtttttgtaa aaccaattgg tttgcatgca tattgttctc ctaaattaat catttcttcc
     1621 gtcatcaaaa tcctcctaaa ctgctggaac ttttctccac attaccactg ctatttcttt
     1681 ggagatttaa ctcattgatt tttatttaca gttaaaaatc aaccttacag gcattatagc
     1741 gtactgtttt cgttttcaca attaaaacag ctgttgtatt tagaaga
//
LOCUS       DAYYFD010000190         1699 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000190 DAYYFD010000000
VERSION     DAYYFD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.196"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(200..1648)
                     /gene="cls"
                     /locus_tag="ACHA25_002928"
     CDS             complement(200..1648)
                     /gene="cls"
                     /locus_tag="ACHA25_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8764155.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT          508 a          366 c          283 g          542 t
ORIGIN      
        1 tactggcttt gaaatgtgtc tcttttaata gcatatatta gcttatttct tgctttcccc
       61 aaaaaacaag agatacccca aagttaattt cgctcatgcg agacgctttc tccatttctg
      121 acgtactctc agaaatggcc ccgttagaaa aggacacgag atctccatct gttgatggtt
      181 ctcatgtcct ttttatgttt taaaggagcg gcgaaatcaa tcttgctaat ccttctttca
      241 tcttgatgat tattcctcgg ttttcataca tttcttttgt gagatgcgta gaaattttcg
      301 agtcttcaaa aaaagctttt cttacacgtg aagccattcg ttcatcataa accagtgtat
      361 tgacctcaaa gtccaagcga aatgaacgaa catcgatatt tgctgagcca actgaaacaa
      421 tgccaccatc gataatcatc gtctttgcat gaataaatcc attctcatat gtttcgatcc
      481 ttgctccata ctcgattagc tctgcagcga aagaatacgt tgcccagtaa acgagaagat
      541 gatctggttt gttaggtatt tggatatgaa cttttacgcc tgaaagaaga gccaacttca
      601 gtgcttcatg aatcgatcca tccggtatgt aataaggcgt ttggatcaat atctcccgtt
      661 tggctaacga aatcattttc aaataagtca ttttgatttg ttcatgttcg gtatctggtc
      721 cgcttgtcac gatttgaaca gctatatcac cagtagagtg aatggacgga aaaaatttgg
      781 gagaatagcc tacttctttt gtatgctgag aattccagtc catcaaaaaa cggttttgca
      841 aggtatacac cgcttctccg taaatacgca aatgattgtc tcgccaataa ccaaatttct
      901 ttaccaatcc tagatattca tttccaacat tgaacccacc tgtatatccg attttcccat
      961 caataactac gattttacga tgattgcgat aattgatccg aggattaata tacggaacaa
     1021 atagaggaaa gaaaaatgcg acttctccgc ctgctttttt caaattctga aaaaagctgg
     1081 aagaaacttg tgtggaaccc catgcatcaa gaaggactct gacttttacc cctcgtcttg
     1141 ccgcctctgt caatgcatct cgaacttctt cacctaatgc atcactgcga taaatataat
     1201 actggaagtg gatatggtct tctgcctgct tgatatcttc aatcagcgca tcgaattttt
     1261 cacgaccatc tgtaaataaa gtgatttcat ttttagtcgt atagagcgta ctttcaaata
     1321 ccgtgagcat atagatcaat tgtttcacat ctacttgtcc agtaggcgga tgaggataaa
     1381 gccctcgcat caatgcctgt ttgtcttctt caatttccag gttcttaccg attttccctt
     1441 ggatcttcaa atcaaagatt ttctctttgg aaatccctct tcctaaaaaa atatataaaa
     1501 taaatcctac aactggaatg aacatcaaaa cgagcaccca tgcccatgtt tgagcagttt
     1561 cctttctttc tcgaaaaacg atgataaagg aaagcaaaat attcataatc aaaaggatcg
     1621 taaaaaaatt gtcaacgata attttcacgg cttcacctct tcttctacca acttaagaat
     1681 acccaagttg ttttttaaa
//
LOCUS       DAYYFD010000191         1691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000191 DAYYFD010000000
VERSION     DAYYFD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.197"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1455
                     /locus_tag="ACHA25_002929"
     CDS             <1..1455
                     /locus_tag="ACHA25_002929"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8764156.1"
                     /translation="VLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGY
                     AISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQ
                     QRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMM
                     AQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMN
                     LILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNR
                     YGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYA
                     VIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILE
                     TYAERKDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQ
                     QVLEKELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1448..>1691
                     /locus_tag="ACHA25_002930"
     CDS             1448..>1691
                     /locus_tag="ACHA25_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8764157.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          590 a          282 c          358 g          461 t
ORIGIN      
        1 gttcttttag cagatgaatc cttagaggaa tatgatacgg taagcaaaca aacgatgttg
       61 tatcgcgaat tactttcaga tgaagaatca aaagaagatc tgattgcaac aatcgttgat
      121 attttggggt atgcgatttc gccagagtat cttttcaacg tcttagcgga tcaagcaaaa
      181 caagcaacgt tccaattgaa tgatctaaac aaagcctttg tgcaattggc atctacctac
      241 aatcaattta atgggttatt tgatgatgtg gatctccaat caaaaaaact aggaacagat
      301 gagcaacaac gaaatgtgac gattaccgaa gtcattaaga aattgaatga cgtggaagtt
      361 ttaggtcacg atggggacgt gatcggggat gcttatgaat ttctgatcag tcaatttgct
      421 tcagaagcag ggaagaaagc cggagaattt tacacgcctc atatggtttc tgatatgatg
      481 gcacaaatcg tgacgttgga tcaaaaagaa cgacgattct ttagtgtttt tgatccaacc
      541 atgggttcag gttcattgat gttaaatgtg cgtaactact taactcaccc agataacgtt
      601 aaatatcatg ggcaagaatt aaatacaacg acatacaatc tagcaaaaat gaacttgatt
      661 ttacatgggg tagatgccga agaaatgaat ctccgcaatg gggatacgtt gaataaggat
      721 tggccaacag atgaacctta cacatttgat gcagtagtaa tgaacccacc ttattcggct
      781 aattggtcag cagatacgac cttcttagac gattctcggt ttaatcgtta tggaaaatta
      841 gcgccaaaat ccaaagcaga ttttgcgttt cttcttcatg gtttctatca tttgaaggaa
      901 acagggacta tggccatcgt cttgccacac ggagttttat tccgtggcgc tgcagaaggg
      961 gtcattcgtc aaaaattact agaagacggc agtatttatg ctgtgatcgg tatgcctgcg
     1021 aacttgttct tcgggacatc gattccaacg acggtgattg tattgaaaaa gaaccgccaa
     1081 actcgagacg tattattcat tgacgctagt cgagaatttg tcaaaggaaa aaatcagaat
     1141 aaattatcgg aagaaaacat tcaaaaaatc ctcgaaacct atgctgaaag aaaagatgtc
     1201 gagaaatacg ctcatttggc aaactttgat gaaatcaaag aaaatgacta taacttaaat
     1261 attccacgct atgtagatac gtttgaagaa gaagaaccaa ttgatatggt tcatgtcgga
     1321 aatgacatca agaagatacg acaagaacag caagtgctag aaaaagaact tttagaagct
     1381 atctcttccc tgcaaacaac acctgaaaat gaagcgtggt tacaaggtgc gctggaggtg
     1441 ttcaaacatg agtaatgata tacaaccaga aattcgtttc ccaggattta cggaagcttg
     1501 ggaacagcgt aagtttgaat gcttacttga taaaaaagat ggagtaagga ggggaccatt
     1561 tgggagtgcc ttaaagaaag aattctttgt atctaatagc aattttgttg tctatgaaca
     1621 acaaaatgct atttatgata attacgaaac aagatataaa attactgaaa aaaaatataa
     1681 tgaattaatc a
//
LOCUS       DAYYFD010000192         1675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000192 DAYYFD010000000
VERSION     DAYYFD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.198"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHA25_002931"
     CDS             <1..1604
                     /locus_tag="ACHA25_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8764158.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          617 a          269 c          346 g          443 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg ataga
//
LOCUS       DAYYFD010000193         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000193 DAYYFD010000000
VERSION     DAYYFD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.199"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA25_002932"
     CDS             131..505
                     /locus_tag="ACHA25_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8764159.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA25_002933"
     CDS             complement(619..1248)
                     /locus_tag="ACHA25_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8764160.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA25_002934"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA25_002934"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAYYFD010000194         1649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000194 DAYYFD010000000
VERSION     DAYYFD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.200"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1604
                     /locus_tag="ACHA25_002935"
     CDS             138..1604
                     /locus_tag="ACHA25_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8764161.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          553 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcctg
//
LOCUS       DAYYFD010000195         1643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000195 DAYYFD010000000
VERSION     DAYYFD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.201"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACHA25_002936"
     CDS             complement(<1..244)
                     /locus_tag="ACHA25_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764162.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQF"
     gene            complement(246..>1643)
                     /locus_tag="ACHA25_002937"
     CDS             complement(246..>1643)
                     /locus_tag="ACHA25_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8764163.1"
                     /translation="SLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKIAFAWGGIIVL
                     YEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLLITLIVIFPAD
                     FSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGFIEH
                     RLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRG
                     AQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFL
                     ATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPT
                     LLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICI
                     YAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFW
                     YYLGSISYLYLKKEN"
BASE COUNT          651 a          270 c          193 g          529 t
ORIGIN      
        1 aaaattgatt atggttagtt aacaagttaa ttttacgttt aaaggaagaa ccgattttcc
       61 ggtgattcac aaaaacaaca tcattaaata gccctaattc ttttatacgc tcactatatc
      121 ttctagcgtt ttcaaattca gaataaataa ataaatctcc ttttgattct cggtaaaaat
      181 tttgataaac atttaatata ttaatcaaat gtaaaggagt ccaagcaatg aaagctgtct
      241 tcatattaat tctccttttt taaatacaag taagaaatgc ttcccaaata ataccagaaa
      301 acaattgaaa tgacatcgcg cttattgaaa agtacatgag cctccaccat ttcatctata
      361 aaaatagata gtataaaaat taaaacaata ccaagaaacg cataattcac tttacttcta
      421 aaaaatgttt tgagtaaact aaagatattt aacacataga aagcatagat acatataaaa
      481 gcaattatac cacttgatac aagtacttga agaaatccat tatgcatgtt tccatgtgct
      541 ctttttagtt cattcacatc tgtagtatca agatctactt ttttcaaaaa ttttgcatcg
      601 ctattcccat agaaatcggt ctctccataa ccaaataaca aagttggtct tataacttta
      661 agcgcagctt tccaaatcgt aaaacgacca gctgaaattt cggagttatc actagaatgc
      721 tgtaatatta cttctttttc tggaagtgga gttttatctg attcatttga ctctataact
      781 tctacttttc tatcaatcga tacaggaatt gttgctaaaa aatccttagc ataattagta
      841 ccaaaagaaa aatataccaa tagaactaat aatatgctta ccatccattt gatttttaca
      901 ggtagtactt ctctaatgct ctttctaaat tttgaattgg caaacaacaa aagaaataat
      961 actaaaaaag aacaaattac tacctgggca cctctagacg aagaaagtgt aatataaaat
     1021 atttgtacga taaaattcat catataaaaa acacgaatcg tttttgttat ttttgaaatt
     1081 aatacactaa ttaaacttaa aataatagaa gtatatccaa acatagctcc aacatttgga
     1141 cttgtataga caccaaataa ccgatgttca ataaaacctt gtcttgcaag taatcctggc
     1201 gttcctgttg ggacatgata agaaatcaaa aaaataaatt gatagataga tattagcgca
     1261 gctaaaaaaa ttatgataat gaaaacatta ttaaaataca ccattttttt ctttttttcc
     1321 tcttcacttt gtgaaaagtc tgcaggaaaa attactatca aagtaatcag caaatatcca
     1381 aaattataaa tagatggaat taaaaaattt tctctgttaa tgattatagt taagataaaa
     1441 ctaatcattg ctccaatcag tagccaagaa aaacgcattt taaaaaaatt tttctttata
     1501 aaaacctcat acaaaactat aattccaccc caagcaaaag ctattttcat cgggatatta
     1561 gttacattaa aagtactagg cataaaataa agcagacaaa tgattaaatt tattactcga
     1621 aacgcatctc gacttaataa act
//
LOCUS       DAYYFD010000196         1506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000196 DAYYFD010000000
VERSION     DAYYFD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.202"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1077
                     /locus_tag="ACHA25_002938"
     CDS             <1..1077
                     /locus_tag="ACHA25_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM8764164.1"
                     /translation="INLLKKEKEDLNDEINQKYDETLKNVYSSLIVSNIEETSSSEIK
                     NKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNID
                     STREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKE
                     QMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEE
                     KLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSA
                     SVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENY
                     NGTVTFTKLAKAEQYRRSLELSKD"
     gene            1149..>1506
                     /locus_tag="ACHA25_002939"
     CDS             1149..>1506
                     /locus_tag="ACHA25_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8764165.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDY"
BASE COUNT          665 a          171 c          234 g          436 t
ORIGIN      
        1 attaatttat taaaaaaaga gaaagaagat ttaaatgatg aaataaatca aaagtatgat
       61 gaaacattaa aaaatgtcta ttcttcactt atcgtttcaa atatagaaga aacttcatct
      121 tctgaaataa aaaataagct ttctttgtta aaattaaaag aaaaggaata tattattgac
      181 gatgctgttt ctgtccctct tattttaaat aaacgagaga aaaataattt aaagaaaaaa
      241 atgctcattc catttgattc agaagtgaaa ggattactca ataaactaac tatttctaat
      301 atagactcga ctagggaaaa aatcataaaa atatttgata aaattaataa tttattcaag
      361 aatgacaatg ctgaattaag gaagggtctt ttagagttta aacttcaaga actagaactt
      421 cattactctt atttagtgaa aattaccgaa gaaaaagaac aacaaaaagc aataaaagaa
      481 cagatgatag aagaggaaaa agttcgtaga gaaattgatc gtgaaaagaa acgtattgat
      541 aaagaacaac gtcaattcaa ttctgaaatt tccaaattaa tcgcatatat gcaaaaagct
      601 aatgcagatg tggaaaaaga attatatgca aataaaattc aagaactgga agaaaaatta
      661 aaagaattag aagttatacg tgaaaatgta ttacaaagag aattaaatac tagagccggt
      721 tatgtgtatg taattagtaa tattgggtct tttggtgaag acatatataa aattggtatg
      781 actagaagat tagaacctat ggatagaata aaagaattga gtagtgcatc ggttccattt
      841 gaatttgacg ttcatgcgat gattttttca gaggacgctc cgtctttgga aactaaattg
      901 cataatcatt ttagaaaaca agaagttaat aaaattaatc aaagaaaaga atttttcaaa
      961 gtttcactag acgaaataga gaaagttgtt ttagaaaatt ataatggaac agtaacattt
     1021 acaaaattag ctaaagctga acaatataga cgatctttag aattgtcaaa agattaaata
     1081 tataaaaagc cttcgggctt tttcttttaa caattaatag gaacgtatat tcgaaaggaa
     1141 tagaaataat gtggttagaa aaaataaatg acaaaaaatt taagtacacc gaacgttata
     1201 cggatccctt aactgaaaaa aagagaaaag tgtccgttac tttatctagc aattcacgcc
     1261 aagcttggaa tcaagctaac ttacttttga atgaaaaaat cgcagaaaaa ataaaacgaa
     1321 atgaagaatt accactaact tttggagaat taaaaactaa atgggatgaa aaatacaagc
     1381 caactgtaaa agaaagttcc tatcgaacaa ctcaggttta tctatcatta atttcaaaat
     1441 atataaaaga tgatgtactt gtaaaaaacg ttaatagtaa tcttatacaa gatatgctag
     1501 attact
//
LOCUS       DAYYFD010000197         1483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000197 DAYYFD010000000
VERSION     DAYYFD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.203"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHA25_002940"
     CDS             64..972
                     /locus_tag="ACHA25_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8764166.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1483
                     /locus_tag="ACHA25_002941"
     CDS             972..>1483
                     /locus_tag="ACHA25_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764167.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          511 a          216 c          345 g          411 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DAYYFD010000198         1422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000198 DAYYFD010000000
VERSION     DAYYFD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.204"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..447
                     /locus_tag="ACHA25_002942"
     CDS             37..447
                     /locus_tag="ACHA25_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8764168.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            461..817
                     /locus_tag="ACHA25_002943"
     CDS             461..817
                     /locus_tag="ACHA25_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8764169.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          524 a          207 c          267 g          424 t
ORIGIN      
        1 tgtagaattg gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct
       61 agctgtactt cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa
      121 gagaagaata tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg
      181 actgaaggag gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc
      241 atagatttta acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca
      301 ttattgagac gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat
      361 gagatccggt catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta
      421 caaaacaata acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg
      481 atcagcgcac tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc
      541 ttatcaattt gttgacttag aagaacgcga tttaagtaca gaagaagcag aagaattatg
      601 cagttttgaa gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa
      661 cattgcgatt gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca
      721 taccaatctc ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa
      781 tgagcagttg atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc
      841 actgagcatt tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc
      901 tttttttgac tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa
      961 atctaggtgg ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt
     1021 catttctgtt gttttaagta gcaaagcaat aaaatagtat tttaaaaaac atataaaata
     1081 gttgttgaca tgttagaaat gacatgataa gataactgag ttgttaaaaa agaaccactt
     1141 gtgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata acaaatgata
     1201 agttatgata aatgagttgc tgtaagattt cgaaaaacga aataaaaaaa acttttaaaa
     1261 aagagttgac aattacttgt ctctctgata tgatataaaa gttgctacgg cagccaaaaa
     1321 cgaatcgggg aacactcctg atgaagtaga cctttgaaaa ctgaacaaag taagacaaac
     1381 caaatgtgta gggcgtcttg attcaattca agacaacaaa ca
//
LOCUS       DAYYFD010000199         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000199 DAYYFD010000000
VERSION     DAYYFD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.205"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACHA25_002944"
     CDS             82..>1364
                     /locus_tag="ACHA25_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8764170.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAYYFD010000200         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000200 DAYYFD010000000
VERSION     DAYYFD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.206"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHA25_002945"
     rRNA            <1..1355
                     /locus_tag="ACHA25_002945"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYFD010000201         1292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000201 DAYYFD010000000
VERSION     DAYYFD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.207"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..674)
                     /locus_tag="ACHA25_002946"
     CDS             complement(<1..674)
                     /locus_tag="ACHA25_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8764171.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFG"
     gene            complement(674..>1292)
                     /locus_tag="ACHA25_002947"
     CDS             complement(674..>1292)
                     /locus_tag="ACHA25_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8764172.1"
                     /translation="KRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSN
                     KKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGT
                     LSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYS
                     HTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          440 a          222 c          140 g          490 t
ORIGIN      
        1 ccaaaataat ctaaatgaat ttctataatt ttatcatcgt atctcaatat atatgttgct
       61 aaatcattac tgttaatttc aagattcgaa tatttaccaa actgtttttt tactgattta
      121 gggaaaccaa ataggtttat aatataatcc aattcatgta tacagtctaa ttctactcct
      181 ccccccatag atttaaaggc agaataatta tttctataat cagtaccttt tctccaatca
      241 ggaagataag tagaacaaat agctcttaca gaatatactt ttttactttg aatactatta
      301 cgaacaactt ccatgatatt tttatacctc agtggagcag caacataaat atttttgtaa
      361 tcataattat attgagtaag atcaatactc tcagaaaaag caggtttttc taaaaatatg
      421 taatcaactt tttcaacaat tacatttagt gtttccagat gcttaaaagt cggatttgta
      481 ataaaagcta tatcataatg tgtatccaaa tcgtctctat tttgaatata cgttatttct
      541 tcaaactctt tttttccact gctactataa gctgttatat caaaattaat agaattcttt
      601 tttccatagc ttattagatt ttttagatgt ctctttccaa ttgaccctaa cccaaaaaaa
      661 attactttca tcattaattt acctcaatgt cgttaattaa gttaataata tacttatctt
      721 tttcaaaagt atattggggt tccattttct tttccaaaag cgtgaaaaat tctttaatct
      781 cctctaaata tgcatcttca ataatagtat gtgaataatt attatcatgc ataacctcat
      841 cgtacaattg aatatctgtc atactagaag tttcttcatt ccatgaaaat agagaatccg
      901 ggttaccttc ccattctatt aaaatctttt caccaattat agttaaattt cttttagcta
      961 ctcttgaaac aacatctaca cttaaagttc caactatacc agtttcatgc tcaataataa
     1021 gagtgaaact gtccgaataa tcaatatcaa gattggataa tttctgggtc attacagtaa
     1081 acgatttaac tttcccaaat gtatctataa tccaaggtaa ttcaatagcg aatatttctc
     1141 tacacccatt agtttttttg tttgaaacaa aaaaattgcg ataatcttcc caaggatgcc
     1201 agtcgggtaa atactgtcct acatggtatt gataagaaat ttttttatta tgcgtcactt
     1261 tagacttaat atattgaatt tcttttctct tc
//
LOCUS       DAYYFD010000202         1280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000202 DAYYFD010000000
VERSION     DAYYFD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.208"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..702
                     /gene="lexA"
                     /locus_tag="ACHA25_002948"
     CDS             79..702
                     /gene="lexA"
                     /locus_tag="ACHA25_002948"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8764173.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            882..1100
                     /locus_tag="ACHA25_002949"
     CDS             882..1100
                     /locus_tag="ACHA25_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764174.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          390 a          238 c          248 g          404 t
ORIGIN      
        1 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
       61 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
      121 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
      181 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
      241 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
      301 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
      361 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
      421 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
      481 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
      541 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
      601 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
      661 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
      721 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
      781 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
      841 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
      901 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
      961 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     1021 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     1081 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     1141 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     1201 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     1261 ggtctttgct tgtttccatt
//
LOCUS       DAYYFD010000203         1246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000203 DAYYFD010000000
VERSION     DAYYFD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.209"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHA25_002950"
     CDS             200..742
                     /locus_tag="ACHA25_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764175.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACHA25_002951"
     CDS             898..>1246
                     /locus_tag="ACHA25_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8764176.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAYYFD010000204         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000204 DAYYFD010000000
VERSION     DAYYFD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.210"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHA25_002952"
     CDS             414..947
                     /locus_tag="ACHA25_002952"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8764177.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYFD010000205         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000205 DAYYFD010000000
VERSION     DAYYFD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.211"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..957
                     /locus_tag="ACHA25_002953"
     CDS             265..957
                     /locus_tag="ACHA25_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8764178.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          526 a          136 c          173 g          360 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatg
//
LOCUS       DAYYFD010000206         1194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000206 DAYYFD010000000
VERSION     DAYYFD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.212"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1027
                     /locus_tag="ACHA25_002954"
     CDS             <1..1027
                     /locus_tag="ACHA25_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8764179.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
     gene            complement(1081..>1194)
                     /locus_tag="ACHA25_002955"
     CDS             complement(1081..>1194)
                     /locus_tag="ACHA25_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8764180.1"
                     /translation="FISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          423 a          176 c          203 g          392 t
ORIGIN      
        1 gttgacaggg aatcctaaaa acattcggct ttttttatat aatcttttta actcaacaga
       61 gataagtttt gtggaattgt atacgctacc tataattata aaaacagaaa aaatgttaaa
      121 agatttaaaa cagtattatc ctctgtctgc ttatgaaaaa aagaaattat tccacttttt
      181 agcaatcaac ctgttggctg aacaagctga ttctttagat attgctagca atcagtcgct
      241 atttttttcg gatataggat tttaccactt aaaaggagta ttaggagaaa aatccgttat
      301 ttttatcaat aagacactct cttggcttta tcttcatgat aaattggatc aaaaatactt
      361 gagaaaaaat gaagatgaaa caatcgaatg gttgaatgaa cgattcattg aaagttttga
      421 aaatcagttt aatctgttgc gagaagagac gagccaattg atccgaagag agttaaccaa
      481 aatacatttt aaacttttgc attacactat taataaagct gatgaatttc cacttgatgt
      541 ttcttttttt agagaaaact atccagcatt ttattgctat ttgatagagt atataaatga
      601 tctaacgtca aaacgcaaag agttgcttca agataaattt tttctttttt tcagttattt
      661 attattattg atcaatcaca tacccattca actgatttct gaaccagtaa agatcatcat
      721 cgatttttct tatggtgctg cctataatca atttatcaag aaaaatttaa gcttgtacat
      781 taatctgaac acggaagtga tagaggcagg aaagcctgag ttcccggatg ttataatcac
      841 gaataggaat aatctatatg aagaaagtgc atctcaagta gtggtttggc tcgatccgcc
      901 aagagcagta gactggggga atcttacaaa gattttgttg actatccaag aagagaaata
      961 caaagacagc aaaattgaca agcaaataga cgattttcct gaagaaatca tcgacaggat
     1021 cgaatagtga acttctttta gaaagtccga ctgctttttc taaaaatatt atttgtcaaa
     1081 ttaagcgaga caaaactttc catttacgta actcaaaaaa acttctaatg gtgtttggta
     1141 atttagtgat tttctaggga tatggtttct tttggacgca acggatgaga tgaa
//
LOCUS       DAYYFD010000207         1194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000207 DAYYFD010000000
VERSION     DAYYFD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.213"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHA25_002956"
     CDS             <1..1076
                     /locus_tag="ACHA25_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8764181.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tctt
//
LOCUS       DAYYFD010000208         1162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000208 DAYYFD010000000
VERSION     DAYYFD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.214"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHA25_002957"
     CDS             <1..969
                     /locus_tag="ACHA25_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8764182.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          394 a          205 c          232 g          331 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt ac
//
LOCUS       DAYYFD010000209         1152 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000209 DAYYFD010000000
VERSION     DAYYFD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.215"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..114
                     /locus_tag="ACHA25_002958"
     CDS             <1..114
                     /locus_tag="ACHA25_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8764183.1"
                     /translation="ILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            119..1066
                     /locus_tag="ACHA25_002959"
     CDS             119..1066
                     /locus_tag="ACHA25_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8764184.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          370 a          170 c          245 g          367 t
ORIGIN      
        1 attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt
       61 ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat
      121 gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt
      181 aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct
      241 tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga
      301 agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt
      361 ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat
      421 gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc
      481 agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc
      541 attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca
      601 tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa
      661 tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat
      721 cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt
      781 ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg
      841 gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat
      901 caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat
      961 ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg
     1021 tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata
     1081 agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa
     1141 atctgggata ga
//
LOCUS       DAYYFD010000210         1109 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000210 DAYYFD010000000
VERSION     DAYYFD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.216"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACHA25_002960"
     CDS             <1..792
                     /locus_tag="ACHA25_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8764185.1"
                     /translation="NLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQN
                     ATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGN
                     IGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLG
                     ERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVS
                     LDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            825..>1109
                     /locus_tag="ACHA25_002961"
     CDS             825..>1109
                     /locus_tag="ACHA25_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8764186.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSG"
BASE COUNT          435 a          121 c          203 g          350 t
ORIGIN      
        1 aatttatggt tagccaaaaa gaatggaatc agacagagaa ttgctcatag ccatgcaact
       61 gaagatggaa aatctcaaaa tattgtaatg aatgtattgc gtaaattttt aaccaaattg
      121 ttgttacaaa atgcaacaga ctaccttgcc gttagtcaag aagctggtga aagtctgttt
      181 caaaaccacc cctttgagat agttcataat ggtattgatt tagagcttta tagtaaaaat
      241 tcagaagcta aggttaataa gagaaaagaa ttggatatct caatgagtac ttttgttgtg
      301 ggaaacattg gacgattaga gaaacaaaaa aatcaatctt atttgttaga gattttttta
      361 gaaatactaa aaatgaattc tgattcttac ttgattttag taggtgaagg gtcacttaga
      421 aataaactga aagccaaagc agtagaattg gatatagaga aaagagtaaa atttttagga
      481 gaaagaaacg atattcccga gttattatct gtgatggatg tttttgtaat gccttctctt
      541 tatgaaggtc tgccaatggt tggattagaa gcacaagcag caaaattaaa attagtctta
      601 gcagatacta tttccaaaga tacaaagtta acaaataatg tggattttgt atcattagat
      661 gaaacaccac aagaatgggc aaaaaaagta ataaaagagc cgtatgctaa taaaataaca
      721 caagaactaa agagttatga tgtttcgtac actactgaac aaatggagag tatatatagt
      781 agagatcatt aaaaatgttc aagatatttt aggagtgatt atttttgaaa atatgttatt
      841 tgatattggc atacaatgat attggcaact taaagagatt ggtcaatcga ttgaacgaaa
      901 atgcagtttt tattattcat atagataaaa aaactgatat agaaccattt attgaagcat
      961 ttaaatcata tgataatgtt gaatttttat gtaataaaaa ccgtgttaaa atttattggg
     1021 gtggtttttc cattgtacaa gcagaaatca atttagtaaa gagagcatta caaaatgaga
     1081 aatatttaaa atatgtatta ttatctggt
//
LOCUS       DAYYFD010000211         1102 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000211 DAYYFD010000000
VERSION     DAYYFD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.217"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(257..961)
                     /locus_tag="ACHA25_002962"
     CDS             complement(257..961)
                     /locus_tag="ACHA25_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764187.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          191 c          199 g          391 t
ORIGIN      
        1 tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa
       61 tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata
      121 ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt
      181 tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt
      241 atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag
      301 gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat
      361 tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa
      421 taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg
      481 aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt
      541 ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg
      601 tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca
      661 cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa
      721 ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat
      781 ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt
      841 gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc
      901 ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca
      961 tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag
     1021 gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc
     1081 cgttttttga aagaaaaata aa
//
LOCUS       DAYYFD010000212         1071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000212 DAYYFD010000000
VERSION     DAYYFD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.218"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..563
                     /locus_tag="ACHA25_002963"
     CDS             <1..563
                     /locus_tag="ACHA25_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8764188.1"
                     /translation="AVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            563..>1071
                     /locus_tag="ACHA25_002964"
     CDS             563..>1071
                     /locus_tag="ACHA25_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764189.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          379 a          137 c          240 g          315 t
ORIGIN      
        1 atgctgtttc aaaaacagaa ggagaaatgg gttcttattg gcaatcaatt gaagatttaa
       61 ttgctgatat gacagatttt attaatgaaa ataaaggcga ttttacggac tggatgaatg
      121 aaaggaaaga agaatttgat cgctggagag aggaacagga gaatagtttc caagattgga
      181 gagaaggaca agaatcagat tacttgtcat ggtttgaatc aatcaaggat attttgaagt
      241 ctatcgatcc aggtggagta atgttagccg aattaatgga tgcacgtgta gatcttcaag
      301 gtgtacgcca tgaatcgatt tctgaacgtt tcctatccga tctaaattat ttgtatcaaa
      361 aaatgaaagc agcacttttc acgattgaat acggtgaaat tgaagtgact gatattcttc
      421 aagacgatct cttttcagat aatcacgaag tcgaaaaaat tgaaacagta gaatttccaa
      481 ttgaagaagg agctttgatt attgcaactg ttgatgatcc aaaacagaat gttttcactc
      541 ttgagaaagt tggggtgatt tgatggttaa agtaaagaga atgatggaaa ctgatgaatc
      601 tggtgttaaa cgtcaattta tgccaattac acatgtctct gcagttttag gcttagaaaa
      661 aatgatatca ggacaatcaa aagttctttc ggttaatggt aaatatggtg cagtcatttt
      721 aacgaaagct gatttaggat tagagaatgc aattactgaa ctaccttatg caagcgagac
      781 aagcgatggt attcttactg ctgaaatgtt tcaaaagatc gtaaatggag aaggtggaac
      841 atatattttg ccgattgcta ctcctgaaca attaggaggt ataaaagttg gggaactctt
      901 agaagttact gaagaaggtg ttttatctgc aactaagcaa acagacttca atttctctga
      961 agaattaaaa ggaaaattag aatctctaaa aatattaaaa gcaggtgcaa atatatcaat
     1021 tgcagaagat ggaacaatta gttctacagg tggtagtggg actggtggtg t
//
LOCUS       DAYYFD010000213         1031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000213 DAYYFD010000000
VERSION     DAYYFD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.219"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..453
                     /locus_tag="ACHA25_002965"
     CDS             61..453
                     /locus_tag="ACHA25_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764190.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(626..829)
                     /locus_tag="ACHA25_002966"
                     /pseudo
     CDS             complement(626..829)
                     /locus_tag="ACHA25_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            795..992
                     /locus_tag="ACHA25_002967"
     CDS             795..992
                     /locus_tag="ACHA25_002967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764191.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
BASE COUNT          370 a          129 c          161 g          371 t
ORIGIN      
        1 acgtaaatca attatttttc attttacttt aactaataat gtttatttgg agggattatt
       61 atgttagatg atagattgac gtgttctaat tgtggtacat tattttacta tgaggaagtt
      121 agaaatattg taaaacatag aaaaaaagaa atgcctatac gttgtccaaa ttgcaatgaa
      181 gttattaagc gaaagttgtc tcatggttat ttcgtcagct acgaactata tgaagaaagt
      241 aaaataaatc tgaaaaaaaa accaaaatac tataaaaatt tgaaaactgg agctattata
      301 actgaaaaag aatttaaaaa tttagtgact gaagaagaaa gcaggtttta taatttgtct
      361 ggtaatattt gccctggatt tatggatcag attattgaaa atgagtcaat tgaatcaatt
      421 gaaaactatg ttcctgttga cttaccaaaa tagaaaaaat aatcctgttt ttacagtgaa
      481 ttattttaat tttgattttc aataaaaata tattacaata ataatcgtaa tagctaatga
      541 aacaatttct caatcttttt accactccaa acagggagcg tcaataattt tgtgtaaata
      601 actcgtcctt ctgcaaaata atgggttact cagtaaacat tgaagctaat gtatccgtca
      661 cttgttggaa gcctttatgg cttctattta agaatttttg attgtatgta tcaaagatgg
      721 atactaggaa acgctctagt gattcttcat tttgaaactg ctcttttctg cggctgtatt
      781 tcttgatttg tttattgaaa gattcgattg tagaaccaga aggaatggag aacacttttg
      841 aactttacaa caaaactact gttgataaat taattccaat tttagttcca actgatttaa
      901 atggtcatga tgttttactt cgttttaacg gatttttagg tgaggattat agatacctaa
      961 aaatttctga tcctcaacct ttaacagaat aataaaaaac gtgtaatcaa ttatttgatt
     1021 acacgttttt t
//
LOCUS       DAYYFD010000214         1021 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000214 DAYYFD010000000
VERSION     DAYYFD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.220"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..460
                     /locus_tag="ACHA25_002968"
     CDS             2..460
                     /locus_tag="ACHA25_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8764192.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="ACHA25_002969"
     CDS             469..696
                     /locus_tag="ACHA25_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8764193.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          166 c          180 g          356 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 t
//
LOCUS       DAYYFD010000215         1003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000215 DAYYFD010000000
VERSION     DAYYFD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.221"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1003
                     /locus_tag="ACHA25_002970"
     rRNA            <1..>1003
                     /locus_tag="ACHA25_002970"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          312 a          198 c          288 g          205 t
ORIGIN      
        1 taagctatga tccagagatt tccgaatggg ggaacccaac atctttaata ggatgttacg
       61 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
      121 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      181 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      241 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      301 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      361 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      421 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      481 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      541 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      601 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      661 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      721 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      781 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      841 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      901 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      961 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYFD010000216         1002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000216 DAYYFD010000000
VERSION     DAYYFD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.222"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1002
                     /locus_tag="ACHA25_002971"
                     /pseudo
     CDS             370..1002
                     /locus_tag="ACHA25_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cc
//
LOCUS       DAYYFD010000217          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000217 DAYYFD010000000
VERSION     DAYYFD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.223"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHA25_002972"
     CDS             <1..>986
                     /locus_tag="ACHA25_002972"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8764194.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYFD010000218          978 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000218 DAYYFD010000000
VERSION     DAYYFD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.224"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..957
                     /gene="thiD"
                     /locus_tag="ACHA25_002973"
     CDS             142..957
                     /gene="thiD"
                     /locus_tag="ACHA25_002973"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8764195.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          354 a          152 c          219 g          253 t
ORIGIN      
        1 attgcaaatg ttttttcaaa taaatttgat taccaagaaa aggtagtggt atatttactg
       61 agggatactt tgaaaaacat atgatagaaa cagtaacagt attatgaaaa atctttttat
      121 aaaaatggag gaatcgattt gatggaagat cagtatgtac aagcaatgac gatcgcagga
      181 tcagatagcg atggaagcgc agggatgcaa gcagatatgc atacattctt tacaagaaat
      241 gtttacggtg tttctgtcat gaccgcatgt gttgcaggta attcttacgg aatcggagca
      301 agtgtgacat tgccaacaga ttttatcgac aaagaatttg aactgattgc gaaggacttt
      361 caaatacgtg cagctaaaac aggtatgcta gcagattcaa agttgattga gacagttgta
      421 aaaaattacc aaaaatatga ttttggacca ttagtcgtgg atcccgtaat cgtaacgaaa
      481 catggaaatc ttttactaga agaatctgct ttgcaaagct tgaaagaaaa gcttgtccct
      541 ttggccgaag tactgacacc taatttttat gaagcagaaa agctgactgg acggacatta
      601 gcagctgaag aagattttgt gcaagcagca aaagaactgc aagatatggg tgcaaaaaac
      661 gttatgatca aaggacatca tgctaaaaat gcgcagcaag aaacagtcaa tgactacgta
      721 ttgttcgaat caggaaaatc attttggtta aatgcaccat tttatcctac cgaccggatc
      781 aacggaactg gcgattcgct atctgcatgt attgcagctg agttagcgaa aggaaaaaca
      841 gttgaacaag cgatccgatt ggcaaaagac tatgtaaacc gtgcaattcg tgaagaaata
      901 aatgtagggc acaaatatgg ccccatcaat cattgggcag ctggtgaatt agagtaaaag
      961 aaagaagttg tgaaaaag
//
LOCUS       DAYYFD010000219          922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000219 DAYYFD010000000
VERSION     DAYYFD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.226"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>922
                     /locus_tag="ACHA25_002974"
     CDS             <1..>922
                     /locus_tag="ACHA25_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8764196.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECLNN"
BASE COUNT          280 a          187 c          183 g          272 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa tgcttaaaca ac
//
LOCUS       DAYYFD010000220          906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000220 DAYYFD010000000
VERSION     DAYYFD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.227"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>906
                     /gene="bsh"
                     /locus_tag="ACHA25_002975"
     CDS             6..>906
                     /gene="bsh"
                     /locus_tag="ACHA25_002975"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8764197.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMN"
BASE COUNT          311 a          123 c          163 g          309 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaata
//
LOCUS       DAYYFD010000221          890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000221 DAYYFD010000000
VERSION     DAYYFD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.228"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACHA25_002976"
     CDS             <1..888
                     /locus_tag="ACHA25_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8764198.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAYYFD010000222          858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000222 DAYYFD010000000
VERSION     DAYYFD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.229"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..117)
                     /locus_tag="ACHA25_002977"
     tRNA            complement(45..117)
                     /locus_tag="ACHA25_002977"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(82..84),aa:His,seq:gtg)
     gene            complement(120..193)
                     /locus_tag="ACHA25_002978"
     tRNA            complement(120..193)
                     /locus_tag="ACHA25_002978"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Trp,seq:cca)
     gene            complement(200..280)
                     /locus_tag="ACHA25_002979"
     tRNA            complement(200..280)
                     /locus_tag="ACHA25_002979"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Tyr,seq:gta)
     gene            complement(282..357)
                     /locus_tag="ACHA25_002980"
     tRNA            complement(282..357)
                     /locus_tag="ACHA25_002980"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Phe,seq:gaa)
     gene            complement(393..465)
                     /locus_tag="ACHA25_002981"
     tRNA            complement(393..465)
                     /locus_tag="ACHA25_002981"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(430..432),aa:Asp,seq:gtc)
     gene            complement(475..547)
                     /locus_tag="ACHA25_002982"
     tRNA            complement(475..547)
                     /locus_tag="ACHA25_002982"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Val,seq:tac)
     gene            complement(563..634)
                     /locus_tag="ACHA25_002983"
     tRNA            complement(563..634)
                     /locus_tag="ACHA25_002983"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(599..601),aa:Glu,seq:ttc)
     gene            complement(649..738)
                     /locus_tag="ACHA25_002984"
     tRNA            complement(649..738)
                     /locus_tag="ACHA25_002984"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(702..704),aa:Ser,seq:gga)
     gene            complement(778..851)
                     /locus_tag="ACHA25_002985"
     tRNA            complement(778..851)
                     /locus_tag="ACHA25_002985"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(815..817),aa:Asn,seq:gtt)
BASE COUNT          239 a          237 c          194 g          188 t
ORIGIN      
        1 aagtcccttg ccttaccgct tggctatacc ccaagaataa aaagggcgaa tgatgggaat
       61 cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca cattcgccat
      121 ggcaggggca gcaggaattg aacccacact aacggttttg gagaccgctg ttctaccttt
      181 aaactatgcc cctataaaat ggaggagagt ggattcgaac cactgaaccc taaggaacgg
      241 atttacagtc cgtcgcgttt agccacttcg ctactcctcc atggtggcgc gggacagaat
      301 cgaactgccg acacacggag cttcaatccg ttgctctacc aactgagcta ccgcgccata
      361 ttttaaaatt attaattgaa taacaagaac gacggttccg acgggaatcg aacccgcgat
      421 ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccagttc attatggagg
      481 ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc agctgagcta
      541 aacctccaac attcaattaa aatgacccgt acgggattcg aacccgtgtt accgccgtga
      601 aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg gagagtaagg
      661 gattcgaacc cttgagacag tttggcacca tctacatgat ttccaatcat gctccttcgg
      721 cctctcggac aactctccag agtgaaagaa gctcaatact ctttctcaaa atgagaactc
      781 cggcagtagg actcgaacct acgacatcat gattaacagt catgcgctac taccaactga
      841 gctatgccgg aataacat
//
LOCUS       DAYYFD010000223          843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000223 DAYYFD010000000
VERSION     DAYYFD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.230"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>843)
                     /locus_tag="ACHA25_002986"
     rRNA            complement(<1..>843)
                     /locus_tag="ACHA25_002986"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DAYYFD010000224          811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000224 DAYYFD010000000
VERSION     DAYYFD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.231"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACHA25_002987"
     CDS             <1..672
                     /locus_tag="ACHA25_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8764199.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAYYFD010000225          785 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000225 DAYYFD010000000
VERSION     DAYYFD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.232"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..198
                     /locus_tag="ACHA25_002988"
     CDS             <1..198
                     /locus_tag="ACHA25_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8764200.1"
                     /translation="DECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVT
                     GKENWNDLYHANEVLKIAEEK"
     gene            199..>785
                     /locus_tag="ACHA25_002989"
     CDS             199..>785
                     /locus_tag="ACHA25_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8764201.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKK"
BASE COUNT          307 a           93 c          139 g          246 t
ORIGIN      
        1 gatgaatgta tcaatgttga cttattaaat aataaaataa gttttgaaga aaacattgtt
       61 aaagaaatgc ctgaatctgc taatgatatg tatgagaagg agttgaaata tttctttgaa
      121 aacgtagtaa caggcaaaga aaactggaat gatttatatc atgcaaacga agtattgaaa
      181 atagctgagg agaaataaat ggaaatttta tttactattt gtggtagagc tgggtctaag
      241 gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc ccctagtcca tttcgcttta
      301 gcaatgatag atatctatca acaaaaacat cctgaacata attgcacgac ttgtttgaat
      361 acggacagta atgagttagt agaattgacg actaagttaa atccgtctgt gaaaattgta
      421 gaacgtaaac ctgaattagc tacagatact gtagggaaaa ttgaagtgat acgtgattca
      481 ttcttacaac tagaaagaga agataatata tttgatttaa ttatagattt agatttgact
      541 tcacctttac gtgaagtttc taatcttgaa gaattaatag atgaacatct aaaaaattta
      601 gataaagacg tctacttttc agttgtttct gcaagacgta atccctattt taatatggta
      661 aaaagaattg aaaatgaagt tactatagtt aataaatcaa gatacacatc taggcaacag
      721 acacctgaag tttttgaaat gaatgcctca atgtattctt ttaaacctga atttctaaaa
      781 aaaag
//
LOCUS       DAYYFD010000226          779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000226 DAYYFD010000000
VERSION     DAYYFD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.233"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            195..770
                     /locus_tag="ACHA25_002990"
     CDS             195..770
                     /locus_tag="ACHA25_002990"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8764202.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
BASE COUNT          285 a           90 c          168 g          236 t
ORIGIN      
        1 ttccttcagt ttttcagatg atataccact caaaaaacaa tatgagagat gttcaggaat
       61 accatctcta atggcttata aattggtttt agggatagta gatgttatag acatatcaag
      121 gaataaacga gcagctgcat aaaagcagat ggaattaagg aataattgaa ttgtaaaaga
      181 agggaataaa tttaatggca caattatttt acagatatgg aacaatgaat agtggaaaga
      241 gtattgagct actgaaagtg gctcataatt atgaagaaca agggaagaga gttatcataa
      301 tgacaagcgc aatagatact cgatcaggat ttggaaaagt tgagagtcgt ataggtgttt
      361 cgaaaccagc tattgcaatt acttctgaaa tggatgtgta cggagaatta gtggagatta
      421 ttgatcagta cggaaaagtt gattgtattt tggtcgatga gtctcaattt ttaagtagag
      481 agaatattat tgatctatca cttatcgtaa tgaatgagga agtaccagta atttgctatg
      541 gattgaagaa tgattttcaa aataatctct tcaagggatc cgaagcatta ttgacatttg
      601 ctgataaaat tgaggaaatt aaaactattt gccatttttg caataaaaag gctgtaatga
      661 atgctcgtat tgttgatggt attccagttt attcaggtga acagattgta gtaggaggta
      721 atgaagatta cctttcggta tgtcgcaacc actactacaa cgaagattga aataaaaaa
//
LOCUS       DAYYFD010000227          758 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000227 DAYYFD010000000
VERSION     DAYYFD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.234"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>758
                     /locus_tag="ACHA25_002991"
     CDS             <1..>758
                     /locus_tag="ACHA25_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764203.1"
                     /translation="NRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKF
                     TNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGA
                     MGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALI
                     IQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIY
                     LGKEFLIGSIDSELLSFGIGLTLLICLIILTFNF"
BASE COUNT          236 a          106 c          110 g          306 t
ORIGIN      
        1 taatcgtgcg ttaagtttag aatctatttt tagctcccct attaattttg aaacgtttag
       61 atcatatggc atgcaagtaa ataattttta cccatggtta actctatatc ctttattttt
      121 attgatcaag tttactaatt tagcaatagg ctataacctt tttctttata ttgttacgct
      181 cattactcta tttatatgtc attatgtaat gtatgaaatt accaaaaaac atgttactag
      241 ctcctttttc gctattatat atacaacatc ttcttttagg agtgtagaaa tatttcttcg
      301 aggggcaatg ggggaattac tcgcaatgtc aatacttcct cttattttac tcgggtttat
      361 aaagctttac gactcaaaaa aagaatcttg ggttatgttg gcgattagca tgactttatt
      421 aatttacact catgttcttt ctgtagctat gactatggta atgattataa ttgctttaat
      481 tattcaattt tatcggaaaa agaaaataga aatcttctta attattaaat taataaaagc
      541 tgctatagtt actttacttc tttcactagc gtttataggt cctatgattg agcagacatt
      601 gtatcaagat ctgaatagac cttatgtgga tatattacaa aaaagagcaa tttatctggg
      661 taaagagttt ttgattggaa gtatagattc agaattacta tcatttggta ttggcttaac
      721 tttgcttatt tgtttaatta tactaacatt taacttta
//
LOCUS       DAYYFD010000228          743 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000228 DAYYFD010000000
VERSION     DAYYFD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.235"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..549
                     /locus_tag="ACHA25_002992"
     CDS             <1..549
                     /locus_tag="ACHA25_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764204.1"
                     /translation="KKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            536..>743
                     /locus_tag="ACHA25_002993"
     CDS             536..>743
                     /locus_tag="ACHA25_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8764205.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNS"
BASE COUNT          266 a          102 c          111 g          264 t
ORIGIN      
        1 aaaaaaattc ccaaaataga tgaaaacagt aaattgatga aaaacataca atcaatcttt
       61 gggatagaag aagtttttgt cggagataaa tgtatctttt ttgatcaacc tttattatct
      121 gatggttatg gcatagatga aacagatatt ttcgctaaag taaatagtat attttctgga
      181 aaaaaagaaa ctttaattaa gcttcatcct agagcaagga caaatcgtta tggtgaaaaa
      241 aacatattga attccagttt accgtttgaa gtcttgctac ttgaaaatga cttctcttca
      301 acacttttga ttagtcctat atctacaatt tctttttcac catctatgat gtttgaaaag
      361 acgatcaact ctattatgtt ggctaaatta attttaaatg aatatacagc attatctgaa
      421 gataaaaaag aaacattgac aaatattgtt cttttctgtg aacattataa ccgtctatca
      481 gaagaaaaaa ttttactccc agaaaactgg gcagaattaa gaaagttggt agagtatggg
      541 attatttaac acactttcca agtcaaaatt agttaagtcc agtttctggt atactatagg
      601 gaattttttt attaagggaa tttcattcat aactattcct atttttgtaa atattactac
      661 tcaagcagaa tatggtttga ttaataattt cacttcattt gtatcaattt tttcgattct
      721 tgttgggcta agcctaaatt cgg
//
LOCUS       DAYYFD010000229          737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000229 DAYYFD010000000
VERSION     DAYYFD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.236"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            441..632
                     /locus_tag="ACHA25_002994"
                     /pseudo
     CDS             441..632
                     /locus_tag="ACHA25_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          290 a           85 c          117 g          245 t
ORIGIN      
        1 taagcctgaa attaagcgtt ttgagataaa agactattct gctttagggc tgttctcaat
       61 tggttactgg tgataaaaaa taagaatact aaaactatac gtagaatata agtaatcggc
      121 atcaatccta attatgtgat tgaatgccga tttttgttac gcgctatttt taattatctg
      181 taagaagaat aattttattt tactcatatt cgttttcact ttgaagaagt aaattgcaat
      241 ttcttataag ttaagcagct atgcaaaagg ctatttattc tattgtgaaa tataagatgt
      301 agttgaaaca ggatatatag aatcaaaata cgaatcttat tttgattttt ctagtttttt
      361 gaaaaaagca ccctttgatc gttgtattag tggaaaagag aaaaaggagc atacttatca
      421 tgtcacaaaa ctacaactaa atatctaaac aacaccaatt tgatgcctat tgaaaaaaag
      481 tattgcgaaa tgaagccaag tctattagaa aatgaaataa tagaataaaa gaaaatggga
      541 aaccgctgaa tgatgtaaat gaaggtaagt atccacgaac taattttgat gaacaagata
      601 tctatttttt atttgtattg gagatactag tttaatatca aaagttatca gcagtcataa
      661 ttagttatcc aatgtaaaaa gtacaaacaa tagttgttat gcagaatagt ttatgaaata
      721 tattacataa taagaaa
//
LOCUS       DAYYFD010000230          727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000230 DAYYFD010000000
VERSION     DAYYFD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.237"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHA25_002995"
     CDS             33..656
                     /locus_tag="ACHA25_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764206.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          254 a          111 c          156 g          206 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatc
//
LOCUS       DAYYFD010000231          714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000231 DAYYFD010000000
VERSION     DAYYFD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.238"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..474)
                     /locus_tag="ACHA25_002996"
     CDS             complement(61..474)
                     /locus_tag="ACHA25_002996"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8764207.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            553..>714
                     /locus_tag="ACHA25_002997"
     CDS             553..>714
                     /locus_tag="ACHA25_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8764208.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLF"
BASE COUNT          206 a          107 c          132 g          269 t
ORIGIN      
        1 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
       61 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
      121 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
      181 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
      241 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
      301 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
      361 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
      421 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
      481 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
      541 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
      601 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
      661 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct attt
//
LOCUS       DAYYFD010000232          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000232 DAYYFD010000000
VERSION     DAYYFD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.239"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACHA25_002998"
     CDS             complement(54..629)
                     /locus_tag="ACHA25_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8764209.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAYYFD010000233          654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000233 DAYYFD010000000
VERSION     DAYYFD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.240"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..>654
                     /locus_tag="ACHA25_002999"
     CDS             208..>654
                     /locus_tag="ACHA25_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM8764210.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          227 a          100 c          130 g          197 t
ORIGIN      
        1 atttttatgg attttttagg atacaaatta attatttttg cttttatcct gattaccagt
       61 ccagtactgt cggtatccca ttttgcagca aaatcatgtt gataaagtga caagaagact
      121 gatgagatat attatccaaa ttgagaagat aattaatcgg attcttgagt ataccctact
      181 ttttaagaaa gtagggtata caagcgtatg aataaagaca taactttccc tgtgatggac
      241 gttcggcttg ttccaattac caaaatacag gcgaacgatt ataatcccaa tcgagtagcg
      301 tctccggaaa tgaacttatt ggagctttct atcattgaag atggctttac tcaaccatta
      361 gtttgttatt acaaggaaaa agaagagaag tatatcttgg ttgatgtgct tcataggtat
      421 cgtgttgcta aggaaagact caacttaaaa gttgtaccgg taacagttat tgataaaccc
      481 atagaagaac gtatggcttc aacaattcga cacaatcgtg caagagggac ccatgaaata
      541 agaggaatgg aaattgtcat ccaaagatta gtaaaaaatg gatggtcaga caagagaata
      601 tccaaagaat taggcatgga cattgaagaa atttttcgct ttaagcaagt gtct
//
LOCUS       DAYYFD010000234          630 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000234 DAYYFD010000000
VERSION     DAYYFD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.241"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..>630
                     /locus_tag="ACHA25_003000"
     CDS             71..>630
                     /locus_tag="ACHA25_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8764211.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIR"
BASE COUNT          234 a          118 c          103 g          175 t
ORIGIN      
        1 tacaaaaaag gaagtccctt ctgtagaata aagttaacga caaccaattc acagaaaaga
       61 ggacttccct atgaatgatt ttactacaga aattgtgcaa actctagtca ctaaaggcga
      121 tttaaatgaa ttattccgtt cgcacttaga aaaagcgata aacacactcc tacggactga
      181 attaacggct tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc
      241 gagaaacggt tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat
      301 acctagagat cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa
      361 cgatacattg gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat
      421 tgctgatttg atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat
      481 gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta
      541 tgtcgctatt tttatggacg ctacttacat tccactaaaa cgtcaaaccg tatccaaaga
      601 agcgatttat attgccattg gtatacgaga
//
LOCUS       DAYYFD010000235          625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000235 DAYYFD010000000
VERSION     DAYYFD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.242"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..625
                     /locus_tag="ACHA25_003001"
                     /pseudo
     CDS             1..625
                     /locus_tag="ACHA25_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DAYYFD010000236          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000236 DAYYFD010000000
VERSION     DAYYFD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.243"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACHA25_003002"
     tRNA            complement(65..150)
                     /locus_tag="ACHA25_003002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACHA25_003003"
     tRNA            complement(167..238)
                     /locus_tag="ACHA25_003003"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACHA25_003004"
     tRNA            complement(250..322)
                     /locus_tag="ACHA25_003004"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACHA25_003005"
     tRNA            complement(340..421)
                     /locus_tag="ACHA25_003005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACHA25_003006"
     tRNA            complement(429..501)
                     /locus_tag="ACHA25_003006"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACHA25_003007"
     tRNA            complement(505..577)
                     /locus_tag="ACHA25_003007"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYYFD010000237          583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000237 DAYYFD010000000
VERSION     DAYYFD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.244"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..451)
                     /locus_tag="ACHA25_003008"
     CDS             complement(<1..451)
                     /locus_tag="ACHA25_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8764212.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          168 a          103 c           71 g          241 t
ORIGIN      
        1 aatttgtaat aagttcgtat atgttttgcg gtgtttcttg tttcttgtaa ttttccacga
       61 atgtttcatc catgaataaa ttcataaacg cttgaagtaa tcctacttgt tcttttggtt
      121 ctttgattcc taaaaaaaat agattttcta cagtcatttc ctgattatcc cccatttgat
      181 aaaaataaat tgggttcttc agctgcataa caaaaacaaa tggcttttcg acatattcac
      241 attccgaatg tggcagcgcg atatttaaat acttagtaat caatccagta ggaaattctc
      301 tttctcttgc aatcagtcca ttcaaataat tctcattgac gtaccccatt ttttctaatt
      361 gttgtgcagc gattaaaaaa gcttcttctt tattttttac ttctaccgct aattgaatca
      421 aactaggaac gaacatctct ttatatttca ttccaacctc ctcctttttg attaagtttg
      481 ttaatttatt gttctactaa attcatcttt gttttacaag aacattatat ttctgttagt
      541 ttgttttgtc aacgctttca aaagaaattt attttgttaa att
//
LOCUS       DAYYFD010000238          582 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000238 DAYYFD010000000
VERSION     DAYYFD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.245"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(178..543)
                     /locus_tag="ACHA25_003009"
     CDS             complement(178..543)
                     /locus_tag="ACHA25_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764213.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          227 a           78 c          111 g          166 t
ORIGIN      
        1 tcaatatcta ttattggaat cattattgct gcaattatta cacaactgtt tttagtgttt
       61 ggagagaaga aaagaagtaa aaaatagaaa aactagtgat agttttggtg atataagaaa
      121 aacatatatg tataaaaaga agaaagtagc ttctctttgt acatatatgg tttgttttta
      181 tggatgccga gcttttttat aagataaggc taccgcccat aacgacccaa ggatttgtgg
      241 aactaagtaa atacctgcaa gaagcaagat cagctgggtc ggttttaaag gatgatagaa
      301 gtaacgccat tcagtaaaat tggcgcaaaa taaataggaa gtgacagtag atataatatt
      361 ggcaatgaat aaagatataa tacgcctgtt cctttgaaaa taatatccag caaaaagaga
      421 taaaaatatc aatgtgatga acccgcttac ggaatgataa cgaaaatcaa gcagtaaggc
      481 gaaatgcaca taaggaacaa aagataaaag caacggaagg tttttccagc aagtttttat
      541 catctttcat caccacctaa caagtgttat tattttgtta gt
//
LOCUS       DAYYFD010000239          571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000239 DAYYFD010000000
VERSION     DAYYFD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.246"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..419
                     /locus_tag="ACHA25_003010"
                     /pseudo
     CDS             186..419
                     /locus_tag="ACHA25_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(392..>571)
                     /locus_tag="ACHA25_003011"
     CDS             complement(392..>571)
                     /locus_tag="ACHA25_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764214.1"
                     /translation="LTPRNKKKFTKIAFQIFVNFGLFPKELLLLPPFTRESVAKLIFR
                     FIPHSSVYLVKNVLP"
BASE COUNT          216 a          107 c           89 g          159 t
ORIGIN      
        1 aatatcaata tccatttcga ggaaaattgt ttaactaaag aaaaatacaa aggacaaatt
       61 tgcttgattt acaaagggac tcttcagtac acaccagaag aatatccaca tcttccaata
      121 tattaaaaga accttcttga atcattgagt gaaagaaaag agattcgtca ctatttgagg
      181 actgtttaac tagagacatt agcgatctgc ctgaaaccta tacaaccact ttacgaacgt
      241 ttaaaaattt tctgccacag attcaaaatg cgcttaactg cagctattca aacagaccat
      301 tggaatgttt aaacaaccac atcaaagtat taaaacgcaa tacgtatggc tttcgcagct
      361 tttacaattt caaactacgg atcatgattc gtcatggcaa aacattttta accaaataaa
      421 cagaagaatg tgggataaat ctaaaaatca gttttgctac agactctcga gtaaacggcg
      481 ggagcagaag caactccttc ggaaataagc cgaaattcac aaaaatttga aaagcaattt
      541 tcgtgaattt cttcttattt ctcggagtta a
//
LOCUS       DAYYFD010000240          561 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000240 DAYYFD010000000
VERSION     DAYYFD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.247"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>561)
                     /locus_tag="ACHA25_003012"
     CDS             complement(<1..>561)
                     /locus_tag="ACHA25_003012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764215.1"
                     /translation="HIWFSIKTLLFIRKEKPEIIYCRIPPNTLVFFIAKFSRCNRVIF
                     DVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSLVITECDYFKKFIKISGESKNM
                     TIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTINNIVDLEAINELLKTCKRKFGN
                     KINLYFIGKGESKTKLADICEKNHIE"
BASE COUNT          188 a           88 c           64 g          221 t
ORIGIN      
        1 attcaatatg atttttttca caaatatcag ctaatttagt tttgctttca ccttttccaa
       61 taaagtaaag atttattttg tttccaaatt ttcttttgca tgttttcaat aattcattaa
      121 tcgcttctaa atctacgata ttattaatcg tacctaaata agcaaggttc aacgtagact
      181 ctaagctttt attgactatt ttctctccat aatcattttg gtcatctaca ttgaaagaac
      241 atggataaat ggtcatattt tttgattctc cggaaatttt tatgaatttt ttgaaataat
      301 cgcattctgt tataactaga ctagaattac tgagatattg atctcgtagc tttttccata
      361 aatcagtaaa aaatttatat ttgttcccta ttcccggcat tgactctggc cataaatcat
      421 acacatcaaa aataactcga ttgcatctac taaacttagc aataaaaaaa actaaggtat
      481 taggtggtat tctacaataa ataatttcag gcttttcttt tcgaataaat aataatgttt
      541 ttattgaaaa ccatatatga g
//
LOCUS       DAYYFD010000241          548 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000241 DAYYFD010000000
VERSION     DAYYFD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.248"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>548
                     /locus_tag="ACHA25_003013"
     CDS             <1..>548
                     /locus_tag="ACHA25_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM8764216.1"
                     /translation="DRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLL
                     KNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDK
                     DPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMK
                     RVSYGFKSFENMRIRIFLINQL"
BASE COUNT          194 a          106 c          101 g          147 t
ORIGIN      
        1 gatcggtttc atattatcaa acacatgaat caagccttta atgagttgcg tatccgtgaa
       61 atgaatgaac ttcgtaaagt aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      121 cgctttttgc taaaaaatcg tgcaaacatc aaccattatg aatacaaaac atggaaaagt
      181 tttcgagcac caaaataccc atttcttacg gaagcgatga tgattgatcg attgcttggc
      241 ttttctacgt ccctgaagga ggcgtatccc tattttcatg aattagttga agcctttcga
      301 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      361 ggctttcggg aaaagctcca aaaccttctg acctatgaag aaggcatcac caacgcgatg
      421 atctatcctt acaccaatgg aaaaatagaa gcgaagaata cccatataaa gacaatgaaa
      481 cgagtatcct acggatttaa atcatttgag aacatgagaa ttagaatctt tttgatcaat
      541 caattaat
//
LOCUS       DAYYFD010000242          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000242 DAYYFD010000000
VERSION     DAYYFD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.249"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHA25_003014"
     CDS             <1..>534
                     /locus_tag="ACHA25_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8764217.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAYYFD010000243          499 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000243 DAYYFD010000000
VERSION     DAYYFD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.250"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..146)
                     /locus_tag="ACHA25_003015"
                     /pseudo
     CDS             complement(33..146)
                     /locus_tag="ACHA25_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          164 a           75 c           92 g          168 t
ORIGIN      
        1 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
       61 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      121 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      181 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      241 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      301 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      361 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      421 atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc
      481 ctttattcta tgctttata
//
LOCUS       DAYYFD010000244          497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000244 DAYYFD010000000
VERSION     DAYYFD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.251"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..497
                     /locus_tag="ACHA25_003016"
                     /pseudo
     CDS             1..497
                     /locus_tag="ACHA25_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
BASE COUNT          165 a           90 c           90 g          152 t
ORIGIN      
        1 actgaagtaa cgcgatacag tgattattgc ttgtgttata tggggcataa ttaatggcta
       61 tactagccaa agagccacgt atagagcggt ttgttccgtc ttatttccta atggtgactt
      121 tcctagtagg agtcgattca ctcgcttaag ttcaaactta gcttacacta tcaagattat
      181 tcgatacttt ttcatcaaga agctcaccaa aggtgaacta gtcggaatta tagatagttt
      241 tcctagtcca ttatgtaaac cagttagaaa tagacaagca aaactattga atcaaatagc
      301 caaagttggc tataactcaa caaaaaaatc ttatttttat ggtcttaaaa ttcatatgat
      361 cgttactaag actggctttc cgattactta ctcaatcacg aatccaggtg tccatgacgt
      421 gaaagtgtta gagactttat cggaagaagc gaatcttccc aatatcctag gggacaaagg
      481 atatattagc cataaaa
//
LOCUS       DAYYFD010000245          495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000245 DAYYFD010000000
VERSION     DAYYFD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.252"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          168 a           96 c           57 g          174 t
ORIGIN      
        1 aaaaataaga atgctaaatc actctacaat aacggcgaaa gcacaaaaaa taaaaaaatt
       61 attacgttat taaaaaaaca gatgaaagat caggttttat tagatctcat tctttcatca
      121 cttgaaggga tttaatgaac tactttttcc tctttgtcgt ttttatatat tcgtattcct
      181 tcaaacagta ggatagcctc tttcaatgct tgatatttga actgtccgtt acttaaaaat
      241 ttagatgtgc cgccctcttc ctagaacata ttctttttta ctttttcatt aattgacaga
      301 ccaatcgaac gaaactgtaa atttcgatag gataatgatt ggtcaaactc attcattttc
      361 aagacatctc gattcatatt caagatagga atctttttat caacatcact ttattcgtat
      421 tttcctctac cataaaagct tcacgctcca caaaattgtt ttatacacta ctattcagcc
      481 cattcaacga ccaat
//
LOCUS       DAYYFD010000246          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000246 DAYYFD010000000
VERSION     DAYYFD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.254"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..>468
                     /locus_tag="ACHA25_003017"
     CDS             43..>468
                     /locus_tag="ACHA25_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8764218.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT          149 a           87 c           85 g          147 t
ORIGIN      
        1 cttgaatttg tatattactt aacaaaagat aatggagtgt gaatgatgga gaccataacg
       61 tattcccctt tgaacttatt tacgaatttt caagaagctg cagctaactt ttccgagacc
      121 ccgattatct ttgatgagcc actttcagct ttcccagaat taggacttga aacaacttac
      181 aaaaaatgct gcgaaagtgt gttgaaacgg gcttatcaat tggctcatct tggagtgaaa
      241 tccggagaca aagttattat ttataagagt tccgcttttg acacatacct tctagctgtt
      301 tctgtgtctt accttggtgc agtgcctgtc atgacttctt atcatctacc aacaacaaca
      361 atggaagtat ttatcgatcg tttagaagac cctttcattt tatttgatga tgagacaaaa
      421 aagcgagtga gagaaatttc aaatggaaca aaatcaaaac agattcct
//
LOCUS       DAYYFD010000247          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000247 DAYYFD010000000
VERSION     DAYYFD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.255"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHA25_003018"
     CDS             complement(<1..430)
                     /locus_tag="ACHA25_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8764219.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAYYFD010000248          459 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000248 DAYYFD010000000
VERSION     DAYYFD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.258"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..400
                     /locus_tag="ACHA25_003019"
     CDS             101..400
                     /locus_tag="ACHA25_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8764220.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          176 a           56 c           92 g          135 t
ORIGIN      
        1 gttttaacaa tccagtaatg atttaaaaaa atctagatgt agcgtatact ttgtttatag
       61 aatgtagtgg gaagaaatat atttttggga ggaaatcgtg atgtctaaaa gtgaagaaat
      121 agaaaacatg gtactaaaat tatatgatct cgttttaaat ccagagatca aagaaaagga
      181 acgtatactt ttgatagacg caaagacagg cttagaaaaa ggtcagtatt atcctaaagt
      241 cataaataat ttggaacgta gtttacgtcc gttagcaatc aggggagaat tgtctaaacc
      301 agtatcaccg ttttatatgg agatatcaac aataggaaaa tttgaaaaag aattaggtcg
      361 aggaatggct tcggcaccga ttacttttgg gcatttataa aaacgagtta ttcatctcaa
      421 tgaattaaaa agtatagtca atctcataat gcaggctag
//
LOCUS       DAYYFD010000249          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000249 DAYYFD010000000
VERSION     DAYYFD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.259"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHA25_003020"
     CDS             <1..>451
                     /locus_tag="ACHA25_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8764221.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYFD010000250          449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000250 DAYYFD010000000
VERSION     DAYYFD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.260"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>449
                     /locus_tag="ACHA25_003021"
     CDS             <1..>449
                     /locus_tag="ACHA25_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8764222.1"
                     /translation="ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITA
                     DDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKE
                     KVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          182 a           67 c          105 g           95 t
ORIGIN      
        1 gaaagtggaa caacagtctt tgcagtgaaa gacggcgtaa gcttaccaga agataaactt
       61 ccagtgttga aagcaaaaga tggttataca gatgcgaaat ggccagagga agcaactcaa
      121 ccaattacag cagatgatac agaatttgta tcaagtgcaa caaaactgga tgataaatct
      181 gacgctgaca aatataatcc tgaaggtcaa aaagtgacta cagaattgaa taaggaacca
      241 gacgcatttg agggaattaa gaacaaagaa gatttaccaa aagatactaa gtatacttgg
      301 aaagaaaaag tagatgttag cgcagctgga aataaaaaag ggactgttgt agtgacatat
      361 ccagatggat catctgatga agttgaagta gatgttacag tgacagacaa tcgttctgac
      421 gccgataaat atgagccaac agtagaagg
//
LOCUS       DAYYFD010000251          442 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000251 DAYYFD010000000
VERSION     DAYYFD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.261"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..431
                     /locus_tag="ACHA25_003022"
     CDS             <1..431
                     /locus_tag="ACHA25_003022"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8764223.1"
                     /translation="VYSLKKYAGKPIIIVNTATKCGLAPQFKQLEELYQAYQTQGLVI
                     LGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTDPLFTYLKNEAPGTV
                     TKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          159 a           87 c           67 g          129 t
ORIGIN      
        1 ctgtctattc attgaaaaaa tatgcaggca aaccgataat cattgttaac actgcaacaa
       61 aatgcggtct agcacctcaa tttaaacaat tagaagaact ttaccaagct tatcagacac
      121 aaggtctggt tatcttaggc tttccttcca atcaatttaa acaagagcta gattcagcga
      181 ctgaagcagc agaagcttgt aaaaccactt atggcgttac cttccctatg catcaattga
      241 ttgacgtaaa tggagaaaat acagatcctc tttttactta tcttaaaaat gaagctcctg
      301 gtactgtcac taaagcaata aaatggaatt ttactaaatt tttgattgat agaaatggca
      361 aagtaatcga acgatttgca ccacaaactt ctccattgaa aatgaccgct gcaattgaat
      421 ccgttcttta acttaataaa aa
//
LOCUS       DAYYFD010000252          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000252 DAYYFD010000000
VERSION     DAYYFD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.262"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACHA25_003023"
     CDS             complement(<1..339)
                     /locus_tag="ACHA25_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8764224.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAYYFD010000253          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000253 DAYYFD010000000
VERSION     DAYYFD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.263"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACHA25_003024"
     CDS             complement(109..>414)
                     /locus_tag="ACHA25_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8764225.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAYYFD010000254          410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000254 DAYYFD010000000
VERSION     DAYYFD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.264"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACHA25_003025"
     CDS             <1..387
                     /locus_tag="ACHA25_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8764226.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           74 c           65 g          113 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa
//
LOCUS       DAYYFD010000255          408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000255 DAYYFD010000000
VERSION     DAYYFD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.265"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>408
                     /locus_tag="ACHA25_003026"
     CDS             <1..>408
                     /locus_tag="ACHA25_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8764227.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL"
BASE COUNT          138 a           67 c           83 g          120 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaa
//
LOCUS       DAYYFD010000256          386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000256 DAYYFD010000000
VERSION     DAYYFD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.266"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          123 a           53 c           56 g          154 t
ORIGIN      
        1 ttttcaaaac attttttctt gatgtgatat ccttgtcttt tttgtaatgt ttttaactta
       61 ttgcttttgc aaaatttatt tacctgtttt ctggatcaac gatagtgtat attatgctat
      121 ccttgtttgg attttaggac gtttaaagag gcactttttt acataactta atccatagac
      181 cttttaagca tgctgtgaga taataataaa gaagagttta aagcgcaccc caaaccactt
      241 ccccataagt gtattacgct ttaaactctt ttatatttga attcactaag aagcatacca
      301 tatttttgaa aaaaagtgag aaaaaaggct tataattagg atttagagta attaattaga
      361 tgtttatttt ataggactga tactat
//
LOCUS       DAYYFD010000257          346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000257 DAYYFD010000000
VERSION     DAYYFD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.267"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           65 c           57 g          111 t
ORIGIN      
        1 gatctgttct aacattctca actttgtcag tttaccatag gaaaatggga aaatctacga
       61 aagctctcaa cagaatcgtc ctacttaaat acgcttttct ttaataataa ataagttggt
      121 tgtttttgtt atttgcgctt tcacttttcg gaataaataa tgtgtattta tatggaataa
      181 ataaaaaaag aggtcgtgac taaaaacaag tcacaacctc tggtaaacgg tgttctcctg
      241 acaactcctc ggtcacaaga cacaatattc aacaaacatg agaagctatt ttttgaaatt
      301 gctctttgtg actcagagtg aaacggcagg ctcacgacct ctttct
//
LOCUS       DAYYFD010000258          338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000258 DAYYFD010000000
VERSION     DAYYFD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.268"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>338
                     /locus_tag="ACHA25_003027"
     CDS             <1..>338
                     /locus_tag="ACHA25_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8764228.1"
                     /translation="FEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHV
                     RYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSF"
BASE COUNT          107 a           66 c           59 g          106 t
ORIGIN      
        1 gctttgaaca atacaatcac atgcctttgg ttatgcgctt aaaaaagcag cgctatacct
       61 gtaaaaactg tcgaacccat tggacggctc aaagttattt tgttcaaccc agacattcaa
      121 tcgcgaatca tgtcagatat aaaattgctt ctttactgac tgaaaaagta tctttatctt
      181 ttattgcgaa aaattgtcag gtatctttga ccaccgttat tcgtacgttg aaagagttta
      241 aaagctactt accaaagcaa tctaagagga ttctccccag agtattgatg gttgatgaat
      301 ttcgttcgca cgcttccata gaagataaaa tgagcttt
//
LOCUS       DAYYFD010000259          330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000259 DAYYFD010000000
VERSION     DAYYFD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.269"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          108 a           45 c           60 g          117 t
ORIGIN      
        1 ctcttttggg aatcagagga aagaatcagg ctttatagag atatttcatc taacaaatta
       61 ctttagactg acagtatata tcagcaggta ctgtcatcgg gaacgtagcc aatatcgtat
      121 ccaaaacagc aggtagagaa gctatttgag gattcaagtt attctatttc aaccatgaag
      181 ttaagttcat taaaaataaa gatagttggt atttcaataa tttttgtata gagttaatca
      241 ctttattgat gctaattcat cttgattggt attataatcg tattcaagtt gcgtggcgtg
      301 cttttgctag aaaatatata actgttcttt
//
LOCUS       DAYYFD010000260          313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000260 DAYYFD010000000
VERSION     DAYYFD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.270"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..187)
                     /locus_tag="ACHA25_003028"
     CDS             complement(<1..187)
                     /locus_tag="ACHA25_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764229.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWL"
BASE COUNT          105 a           59 c           29 g          120 t
ORIGIN      
        1 ggagccattt tttgattttc actaattgat tgctcataaa tctttcactg gacttttgtt
       61 caatcttaaa ttgcaaaact tctaactcat aattcccatc ttctgattta tttttgggaa
      121 caactatcaa acttttataa agtttatcca catcaaaatt tatctttttt gcataatttg
      181 tagccattaa tacatctcct tattcataat aaatttcata tgacgctcct ctcaaaattt
      241 accggtagac tagaaaaaag aataaaagat acgtcaattc acactttatt cttaaaaaaa
      301 aactggtctc tct
//
LOCUS       DAYYFD010000261          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000261 DAYYFD010000000
VERSION     DAYYFD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.271"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHA25_003029"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHA25_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYFD010000262          307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000262 DAYYFD010000000
VERSION     DAYYFD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.272"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA25_003030"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA25_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8764230.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DAYYFD010000263          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000263 DAYYFD010000000
VERSION     DAYYFD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.273"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHA25_003031"
     CDS             <1..>282
                     /locus_tag="ACHA25_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8764231.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYFD010000264          266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000264 DAYYFD010000000
VERSION     DAYYFD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.274"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..>266)
                     /locus_tag="ACHA25_003032"
     CDS             complement(36..>266)
                     /locus_tag="ACHA25_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8764232.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaa
//
LOCUS       DAYYFD010000265          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000265 DAYYFD010000000
VERSION     DAYYFD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.275"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACHA25_003033"
     CDS             <1..>253
                     /locus_tag="ACHA25_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8764233.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAYYFD010000266          247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000266 DAYYFD010000000
VERSION     DAYYFD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.278"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>247
                     /locus_tag="ACHA25_003034"
     CDS             <1..>247
                     /locus_tag="ACHA25_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8764234.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTF"
BASE COUNT           80 a           51 c           61 g           55 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 accttta
//
LOCUS       DAYYFD010000267          235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000267 DAYYFD010000000
VERSION     DAYYFD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.280"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>235)
                     /locus_tag="ACHA25_003035"
     CDS             complement(<1..>235)
                     /locus_tag="ACHA25_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8764235.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT           69 a           45 c           36 g           85 t
ORIGIN      
        1 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac
       61 actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt
      121 gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg tttttctttg
      181 ttttccaagt aagctgtggt ctagtttttc tgatttatcc atgtttttac gtggc
//
LOCUS       DAYYFD010000268          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000268 DAYYFD010000000
VERSION     DAYYFD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.281"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>233
                     /locus_tag="ACHA25_003036"
     CDS             22..>233
                     /locus_tag="ACHA25_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8764236.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEI"
BASE COUNT           87 a           44 c           44 g           58 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat cat
//
LOCUS       DAYYFD010000269          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000269 DAYYFD010000000
VERSION     DAYYFD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.282"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAYYFD010000270          231 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000270 DAYYFD010000000
VERSION     DAYYFD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.283"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..201
                     /locus_tag="ACHA25_003037"
     CDS             <1..201
                     /locus_tag="ACHA25_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM8764237.1"
                     /translation="YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTR
                     KEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT           77 a           45 c           45 g           64 t
ORIGIN      
        1 tacctgaata atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat
       61 cgaagtttac gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat
      121 aagaaaaccc gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta
      181 ttattgggaa tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DAYYFD010000271          228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000271 DAYYFD010000000
VERSION     DAYYFD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.284"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>228
                     /locus_tag="ACHA25_003038"
     CDS             <1..>228
                     /locus_tag="ACHA25_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8764238.1"
                     /translation="LKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSI
                     SNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           82 a           42 c           41 g           63 t
ORIGIN      
        1 cttgaaacca gaaggccgta aggcagttga tattgaaaat tcgattaatg aatggcttca
       61 atccgtaccc aaaaatcttt tcaaatcaat tacctttgat tgtggaaaag aattctctaa
      121 ttggaaaagt attagtaata cgaatgatat tgatatttat ttcgcagacc caggaacgcc
      181 ttcccaacgg ggattaaatg aacattcaaa cggactcctc cgaaaaga
//
LOCUS       DAYYFD010000272          227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000272 DAYYFD010000000
VERSION     DAYYFD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.285"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="ACHA25_003039"
     rRNA            complement(<1..>227)
                     /locus_tag="ACHA25_003039"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAYYFD010000273          224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000273 DAYYFD010000000
VERSION     DAYYFD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.286"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACHA25_003040"
     CDS             <1..>224
                     /locus_tag="ACHA25_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8764239.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAYYFD010000274          220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000274 DAYYFD010000000
VERSION     DAYYFD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.287"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACHA25_003041"
     rRNA            complement(<1..175)
                     /locus_tag="ACHA25_003041"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DAYYFD010000275          217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000275 DAYYFD010000000
VERSION     DAYYFD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.289"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           71 a           31 c           50 g           65 t
ORIGIN      
        1 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat aagataatca
       61 tcatagtttc tgaaggtggt atcgactatg tacttgatat cgtcaatgaa ccgatggtac
      121 tttcttatgc gatggaacag gtagagcgat tgcttgtttg tgcaggagaa caagcgatgc
      181 ggctggtaca gttaagtgaa aaaacaagaa actaaaa
//
LOCUS       DAYYFD010000276          215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000276 DAYYFD010000000
VERSION     DAYYFD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.290"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>215)
                     /locus_tag="ACHA25_003042"
     CDS             complement(<1..>215)
                     /locus_tag="ACHA25_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8764240.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQL"
BASE COUNT           68 a           37 c           35 g           75 t
ORIGIN      
        1 tccaattgtt gtaaggtttc aaatgtgtat tgatagacaa attctacttt caacgactta
       61 taagttgatt cagctacggc attatcaaaa ggacagcctt tatgactcaa tgatcgattg
      121 atgtcaaaag ttgttaataa ttcatcaata gcttggttat caaactcttt tccacgatca
      181 gtatgaaaaa tctcaacctc tgtcagaggt tgttt
//
LOCUS       DAYYFD010000277          212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000277 DAYYFD010000000
VERSION     DAYYFD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.denovo.291"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>212
                     /locus_tag="ACHA25_003043"
     CDS             82..>212
                     /locus_tag="ACHA25_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8764241.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     "
BASE COUNT           77 a           36 c           37 g           62 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat ac
//
LOCUS       DAYYFD010000278        10137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.guided.098, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000278 DAYYFD010000000
VERSION     DAYYFD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.guided.098"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(292..662)
                     /locus_tag="ACHA25_003044"
                     /pseudo
     CDS             complement(292..662)
                     /locus_tag="ACHA25_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            807..992
                     /locus_tag="ACHA25_003045"
     CDS             807..992
                     /locus_tag="ACHA25_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8764242.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1150..1317
                     /locus_tag="ACHA25_003046"
     CDS             1150..1317
                     /locus_tag="ACHA25_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764243.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1935..2594
                     /gene="vanR-B"
                     /locus_tag="ACHA25_003047"
     CDS             1935..2594
                     /gene="vanR-B"
                     /locus_tag="ACHA25_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8764244.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2597..3958
                     /gene="vanS-B"
                     /locus_tag="ACHA25_003048"
     CDS             2597..3958
                     /gene="vanS-B"
                     /locus_tag="ACHA25_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8764245.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(4049..5350)
                     /locus_tag="ACHA25_003049"
     CDS             complement(4049..5350)
                     /locus_tag="ACHA25_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8764246.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            5629..6435
                     /locus_tag="ACHA25_003050"
     CDS             5629..6435
                     /locus_tag="ACHA25_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8764247.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            6453..7160
                     /locus_tag="ACHA25_003051"
     CDS             6453..7160
                     /locus_tag="ACHA25_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8764248.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            7157..8128
                     /locus_tag="ACHA25_003052"
     CDS             7157..8128
                     /locus_tag="ACHA25_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8764249.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            8121..9149
                     /gene="vanB"
                     /locus_tag="ACHA25_003053"
     CDS             8121..9149
                     /gene="vanB"
                     /locus_tag="ACHA25_003053"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8764250.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            9155..9763
                     /locus_tag="ACHA25_003054"
     CDS             9155..9763
                     /locus_tag="ACHA25_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8764251.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(9829..10002)
                     /locus_tag="ACHA25_003055"
     CDS             complement(9829..10002)
                     /locus_tag="ACHA25_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764252.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         2878 a         2084 c         2527 g         2648 t
ORIGIN      
        1 cgcgcagcac gatttttcgc tttctgtttg ccatgcttca ccgtcctttc cattcagggg
       61 tttggggatt tccccaacaa gcaaaacgga cgcggcccgg cagggacggg gcaggccgtt
      121 tttccgggag tggctacact ccctatgctt gctacgttac tgtttctcca ctaacaagac
      181 gtttccaaag ccccaaacta cccgcaaaat agccctctgg aaagaaaaaa gtgcaaaaaa
      241 atagaagcta cgctgtgcag ctcccgctaa aagtaaaaca gaggatgcca gttacacatc
      301 ctccgtttca ttcttcttcg gcttttcaat tagtatggcg ctgtcaatcg cgccctcgat
      361 gatcggcagg tattcttcgg ggcagagttt cagcttgtgg ctgacccttt gccgcagttc
      421 gctttcctcc cgcatgatct ccgggttaaa gtagcgttcc acaggaagcc cgcaggcttt
      481 aataagctgt atgacaaccg gcagactggg aatggcccgt cgttttcgat gttggcaaga
      541 tagcgccagt caatatttat tttctctgcc attgctttgc gcgaaacgcg ctttgcttgc
      601 cgggccgctt tcacatccgc gccgaaagtt tcaaagccgg gacaatcttc aatattcgcc
      661 atatcgcacc tcccacttac attgtatatt tcatactctg cccgtggaat gttggtatat
      721 gcacctttct taaagtttat atttcacatt ttgtttcttt tattcttcga gcaaaagaat
      781 tataatgtac cctgtggagg tgtactatgg actacatgac gttgaaagag gcaggcgaga
      841 aatggggtgt gcccccacga tggataaact actattgtgc tgcgggacgc atccccggcg
      901 ctgtaaagat ggcgacgatc tggctcattc ccaaaactgc ggaaaagccg attgacaggc
      961 gtacaaagaa aggaaaggcg gttactgaat agtataatga aaccggcgca ttttggatat
     1021 ggttttgaag agagagaaag catggtattt tgtcacggaa aaactataag atgatagatg
     1081 gcaggctgct ccagaccgat aaaaagtatt ctcatctgaa acagaaagaa aagatttaca
     1141 catggatgta tgaagaagcg aaactttatc atgacagaaa tggaaaatgc ccgaaacaaa
     1201 gacgaggaag ctgctgctgt atcagccgta tatggtagaa ttgaaaaggc cggcatctgg
     1261 attctttatg gtgaagtctc cagacattgg ataactgctg gtctaatgcg cctttgagta
     1321 tggggtgatt gattttaata ccggggaggt acggcttttt gaggaaaata ttctgggatt
     1381 tacatttggc gattattaca tttcctgtaa ctgcaaggac acggatgatg aagatatgag
     1441 gaatatagac ttgctgattt tctactgtct tgaacggtat taggactgaa tctgctgcgc
     1501 ggagtgcggc aagagcgacc ggagggagta cacacaggcc ctgcctgtgt gaaaagccat
     1561 accgcaaggt gcattcgcaa aaaagaggcc gcgcattttg ctgaggcttg gggtgtcccc
     1621 aacaagcaaa acgagaacgc cgcaggcggg caagtggtgg atcagaatat ccgtaaagga
     1681 tattctgatc cattgcttgc cagtattatc gggatgtttt gacaaccgct gtaatcgaaa
     1741 catcccaaac attaagaagg tgttaattat tattatcttg atttaattcg tttaaaccaa
     1801 taatatagat acatacacca tgaaaagaag cgagtatccc aatgacggat gattaaaatc
     1861 cgaaaagaaa tcgctttgaa actacaggga aactacagac tgttatgtta tcttcttaaa
     1921 atggagggat ttttatgtcg atacgaattc tacttgtcga ggatgatgat catatctgta
     1981 atacagtaag ggcgtttctg gctgaggcag gatatcaggt ggatgcctgc acagatggaa
     2041 atgaggcata caccaagttt tacgaaaaca cttatcaact ggttattctt gatattatgc
     2101 tgcccggtat gaacgggcat gaacttttgc gtgaatttcg tgcgaaaaat gatactccca
     2161 ttctgatgat gacagccctg tcggatgacg aaaaccaaat ccgggcgttt gatgcagagg
     2221 cagacgacta tgtaacaaag ccattcaaga tgcagatttt actaaagcgg gtggaagccc
     2281 tgctgcggcg cagcggtgcg ctggcaaagg aaatccgtgt cggcaggctg acacttctgc
     2341 cggaggattt cacggtactt tgtgacggta cagagctgcc cctgacacga aaagaatttg
     2401 aaatcctttt gctgctggtg cagaacaaag gcagaacctt aacgcatgaa atcattttgt
     2461 cacgtatatg gggatatgac tttgagggtg atggcagcac agtccacact catatcaaaa
     2521 atttgcgggc caagctgccg gaaaatatca tcaaaaccat tcgcggtgta ggataccgat
     2581 tggaggaatc ataataatgg aaagaaaagg gattttcatt aaggtttttt cctatacgat
     2641 cattgtcctg ctactgcttg tcggtgtaac agcaacactg tttgcacagc aatttgtgtc
     2701 ttatttcaga gtgatggaat tacagcaaac agtaaaatcc tatcagccat tggtggaact
     2761 gattcagaat agcgataggc ttgatattca agaggtggca gggctgtttc actacaataa
     2821 ccaatccttt gagttttata ttgaagataa agagggaagc gtactctatg ccacaccaaa
     2881 tgccaataca tcaaatagtt ttagacccaa ctttctttat gtggtacata gagatgataa
     2941 tatttcgatt gttgctcaaa gcaaggcagg tgtgggattg ctttatcaag ggctgacaat
     3001 tcggggaatt gttatgattg cgataatggt tgtattcagc cttttatgcg cgtatatctt
     3061 tgcgcggcaa atgacaacgc cgatcaaagc cttagcggac agtgcgaata aaatggcaaa
     3121 cctgaaagat gtaccgccgc cgctggagcg aaaggatgag cttggcgcac tggctcatga
     3181 tatgcattcc atgtatgtca ggctgaaaga aaccatcgca aggctggagg atgaaatcgc
     3241 aagggaacat gagttggagg aaacacagcg atatttcttt gcggcagctt ctcatgagct
     3301 aaaaacgccc atcgcggcta caagcgttct gttggaggga atgcttgaaa atatcggtga
     3361 ctacaaagat cattctaagt atctgcgcga atgcatcaaa atgatggata ggcagggcaa
     3421 aatcatttcc gaaatactgg agcttgtcag cctgaatgat gggagaatcg tacccatagc
     3481 tgaaccgttg gacatagggc gcacggttgc cgagttgctg cccgattttc aaaccttggc
     3541 agaggcaaac aaccagcggt tcgtcacaga tattccagcc gggcaaattg tcctgtccga
     3601 tccgaggctg ctccaaaagg cactatccaa tgtcatattg aatgcagttc agaacacgcc
     3661 gcagggaggc gaggtacgga tatggagtga gcctggtgct gaaaaatgcc gcctttttgt
     3721 tttgaacatg ggcgttcaca ttgatgatac tgcgcttcca aggctgttca ccccattcta
     3781 tcgcattgat caggcgcgaa gcagaaaaag tgggcgaagc agaaaaagtg ggcgaagcgg
     3841 tttaggactt gccatcgtac aaaaaacgct ggatgccatg agcctccagt atgcgctgga
     3901 aaacacctcg gatggcgttt tgttctggct ggatttaccg ctcacatcaa cattgtaaat
     3961 atttaaaact taaatgattt tgggctcttt gtcaaatagg actgatagag tgagataaga
     4021 acatctaagg tagaattcct tttctacttt agatgttttt tgattcatag gtaatcagat
     4081 tatttagtaa gaaaattctg gtcttcattt tccgaaagct tcgaaagcca taagcgactc
     4141 gtttcaatgc tttgatgtgg gtatgcagat tttctatttt ggcatttgaa taaggcaatt
     4201 cgagggctaa acgaatgccg gattcatagt ttataaaagc tttttttata gaatgcagtt
     4261 cttttggaac ggaattgggc atcgttctta caagtttaaa gaaagtgtcc gcctctttgt
     4321 cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc gattcgtaat tcgtcagaga
     4381 tcgaaagcag acgatccacc acttcacttt cggtgagata cgcccaacca aaattgcgcc
     4441 atttggtcca tttgtcataa acaagcttgt cctgtcgttt gatcagaagc cgagacaacg
     4501 cctttaattg tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag cttttcatga
     4561 ttcgtacgcg aatatcctca aatttacgaa tgagatgttt gacaatgtga aaacgatcca
     4621 caacgagttt tgcctttgga aaacattcct ttatcagaga ggcataagaa acattcatgt
     4681 ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata aaataagaaa
     4741 cgagagcctt tttcttccgt gaaggaagaa tatccagtaa ttttccagta tcgccatcca
     4801 tacaagaaaa ggagtaggga ccgacttgat ttttcagtga acgaaattca tccattaata
     4861 aacaacgcgg taaccaattt ctttttactt ttgtcatggg gatcagagaa gcgagtgtcc
     4921 gagaaacggt cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt gcttgttttt
     4981 cagttagctc ttctaaaatt ttatagcgca cttgtttagc gataaagcag tttctatcga
     5041 caatgtaggt attcgccgta aaataggtgt ggcagccacg acagtggtag cgttgtttcg
     5101 ctaaagcaag ataagtcagt tggttcgcac atttattaag ttgaatgagc gattttttct
     5161 ttccgttctt aacgatcatg ccttcatgat tcatctgttt acacaaagga cagcgacgaa
     5221 tttctttact taacgtcgca gaaacaacaa gactcttgat attcttaatg ctcttcgatt
     5281 ccacatttgt gatgaaaatg tttttatctt tgattccaag tagtagtttg atatgatgaa
     5341 gatcaggcat acagaacgct cctttgattg tgttgtggtg attcaattct aacggagctt
     5401 ctgtatgttt tgttttttca tgctcaaaag ggaccgatag aatgattttc tatcagtcct
     5461 agaaattata gagccgattt tgaccgacag gtataaccct gccggtcttt ttttgctgct
     5521 acaggaaaac tacagactgg ctacagggaa agtacagatg cgcttgccat aataacaatc
     5581 gtaccagcca caaatcgtag ttttattgca aaggaggcat tcgatcaaat ggaaaaaagc
     5641 aactatcatt ccaatgcgga tcatcacaaa cggcatatga aacaatccgt ggaaaagcgg
     5701 gcttttctat gtgcgttcat tatttcgttc acagtctgca cgctgttttt ggggtggaga
     5761 ctggcttccg tattggaggc aacacagata ccgcccatcc ctgcaactca tacaggcagc
     5821 agcactgacg tagtggagaa tttggaggaa aacgctcttg ccaccgccaa agaacaggga
     5881 gatgaacagg aatggagcct gattttagtg aacaggcaga accccatccc cgcacagtac
     5941 gatgtggagc ttgagcaact atcaaatggt gagcggatag atattcggat ttctccctat
     6001 cttcaagatt tgtttgatgc cgcaagaact gatggagttt acccgattgt cgcatccgga
     6061 taccgaacaa cagaaaaaca gcaagaaatt atggatgaaa aaattgccga atataaggcg
     6121 aaaggctaca cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga
     6181 acgagcgagc atcagcttgg tcttgctgtg gatatcaatg cggacggaat tcattcaaca
     6241 ggcaacgagg tttatagatg gctggatgaa aacagctatc gttttggttt tattcgtcgc
     6301 tacccaccag acaagacaga gataaccggt gtgagcaacg agccgtggca ttaccgatat
     6361 gttggcatcg aagctgccac agagatgtac aaccaagggg tttgccttga ggaatattta
     6421 aaaccagaaa aatgagaaaa ggatgttatg ttatggacag aaaaagattg acacagcgtt
     6481 tcccgttcct gcttccaatg agacgggcgc agagaaaaat gtgcttttat gcgggaatga
     6541 gatttgacgg ctgtcgctat gcgcagacga taggggaaaa atcgctttcc catttgctct
     6601 ttgaaacgga ttgcgcatta tacaaccaca ataccggatt tgacatgata taccaggaaa
     6661 acaaggtgtt caacttaaag ctggcagcaa agaccttaaa cggcttattg ataagaccgg
     6721 gggaaacctt ttctttctgg ttgttcctgc atacgccatt gacaattatc cagcgcagag
     6781 gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg gtggatgcaa
     6841 ccatttcaga gggctggatt gatttaaaag tgcgaaacga taccgactgc acctaccaaa
     6901 tatgggtgac cttagatgat gagaaaatca tcggtcaggt gttcgccgac aaagagcccc
     6961 aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt ggcgggattt
     7021 atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa ataatagatt
     7081 gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa agtgtggata
     7141 ttcaggagga gaaccaatga gaaaaagtgt gggcattact gtttttggat gcgaacagga
     7201 tgaggcaaat gctttccgcg ctttatcgcc ggattttcat attatcccta cgctgattag
     7261 cgatgcgata tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg ttagcgtaag
     7321 ccataagtcc gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg gggtaaaata
     7381 catttctacc cgcagcatcg gctgcgatca cattgatacg actgccgccg agagaatggg
     7441 aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt gcggattatg ctttgatgct
     7501 gatgctgatg gccatacggg gtgcaaaacc caccatgcac gccgtggcgc aacaagattt
     7561 cagattggat cgtatccggg ggaaagaact gggggatatg actgtgggag ttattggaac
     7621 cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc atgtgctggc
     7681 ctatgataac agccgaaaaa tggatgcaga ttatgtccag cttgatgagc ttctaaaaaa
     7741 cagcgatatt gttacgctcc atgtgccgct ttgtgcggat acccgccatc tgatcggtca
     7801 gaagcaaatt ggagagatga agcaaggcgc atttttaatc aacaccgggc gcggagcgct
     7861 tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg gtgcggcact
     7921 ggatgtgttg gagggcgagg atcagtttgt ttataccgat tgctcgcaga aagtgcttga
     7981 ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac cccatacggc
     8041 gtactacact gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga attgtctcaa
     8101 ttttgaaagg agtttacagc atgaatagaa taaaagtcgc aatcatcttc ggcggttgct
     8161 cggaggaaca tgatgtgtcg gtaaaagccg caatagaaat tgctgcgaac attgatacgg
     8221 aaaaattcga tccgcactac atcggaatta caaaaaacgg tgtatggaag ctatgcaaga
     8281 agccatgtac ggaatgggaa gccgacagtc tccccgccat actctccccg gataggaaaa
     8341 cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt gatgtggctt
     8401 tcccggtttt gcatggcaaa tgcggggagg atggtgcgat acaggggctg tttgtattgt
     8461 ctggtatccc ctatgtgggc tgtgatattc aaagctccgc agcttgcatg gacaaatcac
     8521 tggcctacat tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa atgattgata
     8581 aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt
     8641 caggttcgtc ctttggcgta accaaagtaa acggtacgga agaacttaac gctgcgatag
     8701 aagcggcagg acaatatgat ggaaaaatct taattgagca agcgatttcg ggctgtgagg
     8761 tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt cggcgaagtg gatcaaatcc
     8821 ggctgagcca cggtatcttc cgcatccatc aggaaaacga gccggaaaaa ggctcagaaa
     8881 atgcgatgat tacagttccc gcagacattc cggtcgagga acgaaatcgg gtgcaggaaa
     8941 cggcaaagaa agtatatcgg gtgcttggat gcagagggct tgcccgtgtt gatctttttt
     9001 tgcaggagga tggcggcatc gttctaaatg aggtcaatac cctgcccggt tttacatcgt
     9061 acagccgcta cccacgtatg atggccgccg caggaatcac gcttcctgca ctgattgaca
     9121 gcctgattac attggcgtta aagaggtgat aagtatggaa aatggttttt tgtttttaga
     9181 tgaaatgttg catggcgtcc gttgggatgc caagtacgcc acatgggata acttcacagg
     9241 aaaaccggtg gatgggtatg aggtgaatcg catcatcggc acaaaggccg tggcgtttgc
     9301 tctgcgcgag gcacaaatcc atgcggctgc gcttggctat ggcttgcttt tatgggatgg
     9361 atatcggcca agaactgcgg tggactgctt cctgcgttgg gcagcgcaac cggaggacaa
     9421 tctcacaaaa gaaaaatttt accccaatat agagcgagcc gagttgatta caaagggtta
     9481 tgtggcctca caatccagcc atagccgtgg aagcgcaatt gatcttacgc tctaccacct
     9541 ggatacaggg gaacttgttt caatgggaag taacttcgat tttatggacg aacggtcgca
     9601 tcatacagca aaagggatag gggatgcaga ggcacaaaat cgaagatgct tgcgtaaaat
     9661 catggaaagc agcggatttc agtcttatcg ctttgaatgg tggcactata agttgattga
     9721 tgagccatac cccgatacct attttaattt tgccgtttca taatgaaagt attggttttg
     9781 tatcagattt tggatttgat aaaatctatg ctggtcatgg acaatctttt actggaaagt
     9841 ggttaaagca tcttttgtaa tacattgcaa attatagtat tcaatctgcc cagcggcaga
     9901 aaagaaaaaa accgctgttg ggcagacctt tcccacgccc attttgtatt ttgctggcgg
     9961 cggttttgag aaatcaaggc cgccgttctt ttctggcagc aaaaggtgtg gcggcccata
    10021 ggaggatgcc gctatgctgt tttgctattg ccatgctcta atccacttcc accactcaaa
    10081 aaaagccttg cccctccatg aactgaaccc caaaagttga actatttaat ggactgt
//
LOCUS       DAYYFD010000279         9501 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.guided.102, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000279 DAYYFD010000000
VERSION     DAYYFD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.guided.102"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..1096)
                     /locus_tag="ACHA25_003056"
     CDS             complement(155..1096)
                     /locus_tag="ACHA25_003056"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8764253.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1222..2343
                     /locus_tag="ACHA25_003057"
     CDS             1222..2343
                     /locus_tag="ACHA25_003057"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8764254.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2601..4103
                     /gene="eat(A)"
                     /locus_tag="ACHA25_003058"
     CDS             2601..4103
                     /gene="eat(A)"
                     /locus_tag="ACHA25_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8764255.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4170..4697
                     /locus_tag="ACHA25_003059"
     CDS             4170..4697
                     /locus_tag="ACHA25_003059"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8764256.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            4908..5969
                     /locus_tag="ACHA25_003060"
     CDS             4908..5969
                     /locus_tag="ACHA25_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8764257.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5972..6868
                     /gene="murQ"
                     /locus_tag="ACHA25_003061"
     CDS             5972..6868
                     /gene="murQ"
                     /locus_tag="ACHA25_003061"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8764258.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            6882..8864
                     /locus_tag="ACHA25_003062"
     CDS             6882..8864
                     /locus_tag="ACHA25_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8764259.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(8929..9375)
                     /gene="nrdI"
                     /locus_tag="ACHA25_003063"
     CDS             complement(8929..9375)
                     /gene="nrdI"
                     /locus_tag="ACHA25_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8764260.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3089 a         1689 c         2075 g         2648 t
ORIGIN      
        1 aataaaaaac ctacattttg aagagattat gtactgaaac aaacaagcac aattcttatg
       61 gcatcatgag agttgtgttt tctttttgat cttttattgg gcttaaaaaa agagaaccag
      121 ctgctgattc agctgattct catcagagag ccttttattc tgcgccaagg attccttctt
      181 ttgcatggac taattgggtc aaaaaggcac aatatggtgt agcaatatca tatttttgtc
      241 cttttcgcca aatggcacca ttgatgtagt cgatttcagt caaacgatgg tttttgatca
      301 ggtcttggta catagatggg taatgcaacc cgatgttgtc aggatcataa gtagaagcaa
      361 tatgttggta cacttcttct tgatctagta tgattccttc ttttgctgcg atagaagcaa
      421 attcagatac gattgttcga accatgctct ctgccgcttt ttgcgcgcct agttcagcaa
      481 tattacaatc caaaatcgtg cataaaccat tcaatgttcc atttacacaa gcttttcgcc
      541 aaatggaata acgaacgtta tggctataga tcggatttaa atttgcttct tgaaagactt
      601 ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa gttctctagt tcgatttctc
      661 catcacctaa caatgtgact tttccgggac cagctagacc agctgtccac atcgtgatcc
      721 caaataagat atttttcttt ggcacatatt tttccagtac atcttcatga cctaatccat
      781 ttagtaaaca caaaacataa gtattgtcag taataatgga ttggatcgaa caaaacatat
      841 catctaattg attggctttt gttaatgcta cgatcaagtc cacgtgttcg ttgctttcga
      901 tgatttcttc tggtgaatag attggcagtt ttgcgaccac ttctttaccg ttaaagtcag
      961 caatcaaccc atttgtacga atggcttcaa tgtgagcagg ccaacgatcg atcaagagga
     1021 cctcatttcc gctttgatgt agcatcaatc cgattcggct gcccattgct ccggccccag
     1081 ctattgcgat tttcatgttt tttctctcct ttattcaatg agttcccttt tagtatatag
     1141 aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat gaaaaaaagt gtcttactta
     1201 agaagaaagt gagggataca gatggatatt ttggcggcaa ttgattcatt taaaggaagt
     1261 gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg taaaggaaga
     1321 aaaatcaatg tgccgattgc agatggcgga gaaaatactg cagaagcaat ctattacgcg
     1381 ctcaggggaa catggcgtac cattgatggg atggatctct tattccgtca gcgaaaaatc
     1441 agctatttat tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc tgcacaagca
     1501 ttggggttag atctacttga taaaccaacg gatcaaacta tccgacttgc ttccagttat
     1561 gccttaggag aacttattaa agatgcactg cttcaagaag tagaacaaat tattgtcact
     1621 ttaggaggaa gcgggtgtag cgatggtggt ctaggattac tccaatcctt gggtgcttct
     1681 ttagaagggt acacagaagg aaatccttta ttatctacta aaaaacttca ttttgatcga
     1741 agaaacgttc ttgtttctaa agtaaaattg attatcgctg cagatgttac caatccatat
     1801 acaggtcaac aaggagctgc ttggcaattt ggaagacaaa aaggaggaat agatacgacg
     1861 ctagctttct tagataaaca agcaggaaag attgctcagc aaataaaaga tttctgggag
     1921 gtggatttag atgatattcc aggaagtgga gcagcaggcg gtattggtgg agcccttttt
     1981 ttgttgggag cgaagatggt ttctggtttc gatttagtca gccaaatcac tggattggaa
     2041 aaaaagatcg aacaagcaga tgtcatcatt acaggagaag ggcggatcga tcagcaaacg
     2101 atccacggaa aagttccata cggtgtagca gtgcttgcaa aaaaatatcg aaaaccaata
     2161 atcgctattt gcggaagtag agaccaagag ctagaaggac tttctgattt tctcccaatt
     2221 gtcttaagta ttcatttggg tccaataggt ttagaagaag caatggaaca caggcaaaca
     2281 ttagaaaaaa caaaaatctt aggacagaca cttagcagat ttttcaccat cctcccgaag
     2341 tagttgacaa aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg
     2401 atccgtgcat tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg
     2461 agacaaggaa gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct
     2521 ttttgtgtgt atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt
     2581 tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg
     2641 acagccaagg cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac
     2701 taggacttat cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca
     2761 aactacctta tcaagggcaa gtaatccatc agcaagaatt tgcctatttc ccgcaacaga
     2821 caaaagataa agaacgttta acctattacg tgttaaatga tattacggat tttgagatat
     2881 gggaaatcga aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat
     2941 tcagcacact atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg
     3001 acactcattt cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac
     3061 aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg
     3121 gatttatcga tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt
     3181 atcaagggaa tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg
     3241 ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag
     3301 ccgaatggtc tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata
     3361 ctgaatctag acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac
     3421 gatccaaagc aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa
     3481 aagatatcga atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac
     3541 ttttaagcgt agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt
     3601 ttacaatcga gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat
     3661 ccattatcca ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc
     3721 caaaacatct gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg
     3781 cggaatttgc agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc
     3841 ttgggatgga aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga
     3901 aaaaagtgga attggctaaa tctttatcac agccagctga actatataca tgggatgaac
     3961 cattgaatta tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga
     4021 aacctgccat gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga
     4081 ttatttctct tgagagaatc taacccttgt aatctcaaga acaacaaggt atggttaagg
     4141 ggaactttgc caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa
     4201 cttggaacgc acaaggatta gtttgcggac gggcagatgt gccgctgact gaaaaaggac
     4261 agatgcaggc acagaaacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa
     4321 tcatccattc ccctcttcaa cgagcaagag atacagcaca agcagttgct gatcgtctca
     4381 gcttgccgct gaccgttgat gaacgattgg tagaaatgga ttttggggat tatgatggac
     4441 tgccaagcaa agatgagaac tttcaaaaag cacgtttagc gttcgctgta cgattcccaa
     4501 atggggagtc agtcttagat gtgtatgcac ggatcgttcc tttattgaaa gaatgcatcg
     4561 aagacgaaga aaatgtctat ctgttagttt gccataatgc cttgataaga gtgatcaatg
     4621 cttattttca tccgatgcct aatgaggggt tctttacatt catggtagat aatacagaat
     4681 tggtttctta tgagtaatta tgtttttgtg ttatttaaat aaaatatttc aaggcactgc
     4741 ttttgcttag cagtgccttt tttaggttat gacaattctt ttgataatat aaactcaacc
     4801 tttagaaatg aaatttaatt attttaataa aagtattgaa attttgtttc tttagatata
     4861 tactctagtc atagaaatga agacggatac agaaaaagag ggataatatg tttggctttt
     4921 caatatttat gaacgattct ttgtcagaag agaagaaaca gtatatccgc caaatggcat
     4981 ccaatggctt tgtcggtatt ttcacttcca tgcatatacc agaagatgat gtttcggcat
     5041 atcaaaagcg attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta gaattgatgg
     5101 tcgatatttc aggagaggca ttggaacgag cgggtttttc attagaagat ctaacgcctt
     5161 tgaaagcaat tggtgtaaca ggtttaagaa tggattatca tatttccaat cagcagattg
     5221 ccgactggtc acatcaattg aaaatcagtt tgaatgctag cacgatcacg cctaaagata
     5281 tcgatgaatt gaaagaagca gaagctgatt tctcacagat tgaagcttgg cataattatt
     5341 atccacgtcc cgaaacaggg ctggacaaag aatggtatca gaaaaaaaat caatggctca
     5401 aaattcaagg tctccttgtc caaggttttg taccaggaga tacagagttg agaggcccac
     5461 tatatcaagg attgccaact ttggaagaac atcgagggat ccatcctctt gcaggcgctc
     5521 tagatctatt agcaagtaac acggatattg tttacatcgg agatgcaggt ctgtcagaaa
     5581 acgttcagga gcaatttgct tctttccaaa aagaacagac agtcttgctc catacggaac
     5641 ctgtagatga agaattttac gaatatatat tagggaagca tacgaatcgg caagacgatg
     5701 caagagatgt catacgaagc gtagatgcac gttttcgaga gattccgcct atcccagcaa
     5761 gaaatacagc tacgcgtatg aaagggtcta ttactttaga taacgaaaag tatttgcgct
     5821 atatgggtga gatccaatta accaaatatg atttgccagc ggatgaaaaa gtgaatgtag
     5881 tagctaaagt tatcaaagaa gagctaccat tgatcaacca aatcaaagct ggtatgaact
     5941 atcaatttat aagaaaagaa ggaagataaa aatgagcaaa atcaatttag agaacctaac
     6001 aacggaacgc agaaaccaag aaacttttgg attagatgag atgagtattt ctcaagccgt
     6061 tcaactaatg aacaaagaag atcagaaagt ggccgctgcg gttgaaaaag aactgacaag
     6121 tatcgaacca gtgattgcta acacgattga gtcctttaaa aaaggtggac gtctgattta
     6181 tatgggagcc ggaacaagtg gaagactagg tgtgttagat gctgctgaat gcgtaccgac
     6241 attcggtgta gaaccggaaa tggtcatagg tctaattgct ggtggagaag aagcaatgac
     6301 tgtagctgtc gaaggggccg aagattctta tgagctaggg caagaagatc taatcaagct
     6361 gaatttgaca aaaaatgata tagttattgg tatcgcagct agcggtcgta caccatatgt
     6421 gatcggtgga ttagattacg caaaatcgat cggtgctgtc acaggaacga tttcttgcaa
     6481 taaacaagcc gaaatcagta aacacgcgga tttcccaatc gaagtcgatg caggtccaga
     6541 atttttgaca ggctctactc gattgaagtc aggaacagca caaaaactta tcttgaatat
     6601 gatttctact atcagtatga ttggtatagg taaagtattc aataacttga tggtcgatgt
     6661 caaaccaaca aatgaaaaac taatcgaacg ctcaaaacga atcatcatgc aagcgacaga
     6721 agctgattac gaaacagctg aaaagtactt tgaagaagca gatcaaaatg ttaagctggc
     6781 tatcgtcatg attttgacaa atagtgataa agaggaagct caagcaaaat tagctgaagc
     6841 aaacggattt gtaaaaggga ccttatgagg gggaaaagac catggcaaaa aacgaactga
     6901 ctttggaaga actggcaaaa caaatttata aaggggcagg cggcatgggc aatgtagaaa
     6961 gcattgtcca ctgcatgact cgtgtgcgta tgaagatcat caatgatgat cttgtaaatg
     7021 tctctgaatt aaaagcgatt ccaggagtgc ttggtgtagt agaagatgaa cagcttcaag
     7081 tcatcatcgg acctgggaaa gtgaacaaag tagcacagca aatggttgat ttggcaggcg
     7141 tgaaattagg agaaaaactt tcttctgtaa gtacagctac agcaaattca tcagcatctg
     7201 gaaaagaccg tgtaaacgaa cgggcacaag aaatgaaagc ggcacaaaaa tcgaaacaaa
     7261 aaccatctaa aatcaaatcg gtattaaaag acatttctaa tatctttgtg ccaatgattc
     7321 ctgcatttgt cggtactggg atcgtggcag ggattgccgc agttttagca aacttagtca
     7381 cagccggaac acttgatgca gcaacatggc aacaatatat cgatgtgatg aacatcttga
     7441 aaaacgggat gtttagctac ctagttatct atacaggtat caatgctgca caagtttttg
     7501 gtgcaacacc aactcttggt ggagttatcg gggccgttgt gatgctgaca gggatgaatc
     7561 cggaagcacc actgaaaaac ttatttactg gtgaagctct tgcagctgga caaggtggga
     7621 tcctaggtgt catcttcgct gtttggctat tgtcaatcgt tgagaaaaaa ctgcataaag
     7681 tagtacctga tgcggtagat atcatcgtta caccaactct ttcattgatt gccatcggtc
     7741 tgattgaaat tttcttcatc atgcctttag caggtttcat ttctgatggc atggtcggtg
     7801 ggatcaactg ggtacttggc gttggtggag catttgccgg cttcgttcta ggcacattat
     7861 tcttgccgat ggttatgttc ggactgcatc aaattttgac tccaatccat gtgcaaatga
     7921 ttgatgaaac tggtcgtacg ctattgcttc caattcttgc gatggctggt gcaggacaag
     7981 tgggtgcagc attggcatta tggattcgct gcaaaaaaga taaagaatta acagaaatga
     8041 tcaaaggagc tttaccagtc gggatcctag gaatcggcga accattgatt tacggagtaa
     8101 cattacctct aggacgccca tttattactg cttgtatcgg tggaggtatc ggcggggcag
     8161 tgatcggaat gatcggaaat gtcggggcta tcgctattgg accttcagga gccgctttga
     8221 tcccattgat ctctgacggt aaatggttag gctacatcct tggattattg gcagcttatg
     8281 ctggtggctt cgttgctaca ttcttcttcg gtattcctaa agaccgttta gaaaaagaag
     8341 aattagcgac tgaaacagcc acagtaaatg aagtgcctga aaaagtagta cctcaaactt
     8401 cagctgaaag tgtgactatc ctctcatcag ttgctgacgg aacagttgtt tctttagata
     8461 aagcaagtga tccagttttt gcacaaaaga tgatgggtga aggctacttt gtagaaccag
     8521 aaaacggaca tatctattct ccagttactg gggtagtcag ctctgtcttc ccaacgaaac
     8581 acgctattgg gatcacaacg ccaaatggtt tagaaatctt gcttcatatg ggaatcaaca
     8641 cagttgactt aggcggtacg ccatttgatg tgaaagtaac agaagggcag caagtgacac
     8701 ctgatacgct tgtagcagaa gctgacttag aagcgatcaa agcagcaggc aaagaaacat
     8761 ccatgatcgt cttggtaacc aatatggatc gtgtaaaaaa ctttgtcttg gaaaaaacag
     8821 gtaaagcaaa agcaaaagca cctgtgatgg atgtcaaagc ttaatatgat agaaacaata
     8881 gaaacgaaag aagaaactga aatctatttt tcagcttctt cttttttatt atttattgtt
     8941 ttctgctaaa atctgataaa ttcgtgtcac atcagatggt gttccccgta attcataatc
     9001 tgccaagaaa ggaaagccaa atgtttgtgc atattgtttg gctgttagac aatactgatt
     9061 attgaagttt ttatttccgc taccgactac accaagacaa agtttatgat tgtcttcatg
     9121 ttccagatat tcacgcatcg tttcggtcaa gatttctgta tcaccgttat ctaaaccatt
     9181 tccgccgtct aaataagtag gaacgaaagt aaaaaatggt tctgtttctt tcgtaaaatc
     9241 actattttca tggatttctt ttaaagtaac ttcaggattg acaggatctt ctgcatgcat
     9301 tttttcagca tattctgcta gatgtttggc aaaggcacgc gtatttcctg aaatcgatat
     9361 atataaaatg ttcatggatc atcctccaat tctttccttt tagtatagca tcttttttaa
     9421 gttttttggt ggtgtaattt ggaaagggac taggcgcttt gcttgtataa tgagaaaaaa
     9481 gccggtagaa ttatatttct a
//
LOCUS       DAYYFD010000280         8845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.guided.109, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000280 DAYYFD010000000
VERSION     DAYYFD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.guided.109"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..425)
                     /locus_tag="ACHA25_003064"
     CDS             complement(<1..425)
                     /locus_tag="ACHA25_003064"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8764261.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAV"
     gene            complement(418..1557)
                     /locus_tag="ACHA25_003065"
     CDS             complement(418..1557)
                     /locus_tag="ACHA25_003065"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8764262.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(1762..2955)
                     /locus_tag="ACHA25_003066"
     CDS             complement(1762..2955)
                     /locus_tag="ACHA25_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8764263.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(3034..3240)
                     /locus_tag="ACHA25_003067"
     CDS             complement(3034..3240)
                     /locus_tag="ACHA25_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8764264.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(3233..4375)
                     /locus_tag="ACHA25_003068"
     CDS             complement(3233..4375)
                     /locus_tag="ACHA25_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8764265.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            4887..5522
                     /locus_tag="ACHA25_003069"
     CDS             4887..5522
                     /locus_tag="ACHA25_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8764266.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            5658..5750
                     /locus_tag="ACHA25_003070"
                     /pseudo
     CDS             5658..5750
                     /locus_tag="ACHA25_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            5864..6499
                     /locus_tag="ACHA25_003071"
     CDS             5864..6499
                     /locus_tag="ACHA25_003071"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8764267.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            6606..7844
                     /gene="mef(H)"
                     /locus_tag="ACHA25_003072"
                     /gene_synonym="mefH"
     CDS             6606..7844
                     /gene="mef(H)"
                     /locus_tag="ACHA25_003072"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8764268.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            8071..8490
                     /locus_tag="ACHA25_003073"
     CDS             8071..8490
                     /locus_tag="ACHA25_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8764269.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            8495..8734
                     /locus_tag="ACHA25_003074"
     CDS             8495..8734
                     /locus_tag="ACHA25_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8764270.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         2710 a         1572 c         1649 g         2914 t
ORIGIN      
        1 acagccagct gctcacggat gaggtgctga gttgttccat cttcattaag aatcttaggt
       61 gctaacatcg cacattccgg atgttctttc aataacaaga ccaatgctgt aactgtctct
      121 tcatccacta aaatatctgg gttacaaata atgccatagc gagaatcgct agctatcaga
      181 ttgtgattat gtccataacc aaatccggaa ttttcatcat gaaaatagat atcgatgtag
      241 ggatatttcc tatactctgt tagtcttttt ttgtactcgg gaagtgaatt gttgtcaaaa
      301 attcggattt ttactgctgt ttcatctttt acagttgaaa taaaattatc tagtgtccga
      361 aaaattttat cactgtttgc cgtcacaata ctaataagga tcggctggtt taatttgtta
      421 ttcatctata tgacggttcc atttcttcaa atatttctgt tttacttgcg tacggtactc
      481 tttattccct aaaaatcgca atgtttttaa aataaactga tgtttctcat tgatcagacc
      541 aatcaattca aataataaaa ttgcagcaaa caacatggca ataatcaata tgatcgttcc
      601 gattcttcca gtataattca tgaccatagc tgtgaaagaa aacatcaaag ccatcgcata
      661 aatcgtcagg acagcacctt tatgagtaaa gcctaaactg attagtcggt gatgaagatg
      721 ttttttatct gctgtagaaa aagaaacacg gtttgctttt ctcctaatga tagcaaaaaa
      781 cgtgtctgtt accggcactc ccatgacgat cagcaaagaa atcgatgaaa caaaagtcac
      841 gttcttcagc ccttgtagtg aaaggacggc catcataaac cctaggaata gagctcctgt
      901 atcgcctaaa taaatctttg ccggataaaa attgtaagga aaaaaaccga taatacttgc
      961 tacaagacag aaaataacaa ctggaatata cactgtagaa gcatggagga aaaaatatcc
     1021 aatgattcct atcgtaatta aaccgatcaa tgaaatccct gccgccagcc catccaaacc
     1081 gtcaatcaga ttgaccgcat tcgtgatacc gaagatccaa aaaatcgtta cgggaaaact
     1141 aagccagcct agttggatat atccaataaa aggaagcgtg aaggaatcaa tacgtatatc
     1201 tgctacaaaa taaataataa gagaagcaat caagatccct atactttttt gtttaggtgt
     1261 cagctcatat ttatcatcga gcactcctgt gactatgatt actaaagaag caatcaagat
     1321 cggtacaata tacgattgag ggataatttt acggaataag aataaagtcg atataacaaa
     1381 tgaaataaag ataggcagtc cgcccgctga aggcattgga actttattta ttcttctttc
     1441 atttggtttg tcgaccatac ctgtttgtac tgaaatgatt ttgaatacag gtgttaatat
     1501 cagtgataat aaaattgtca gaaaaaatct tataaccata ctaaaagaaa caagcatatg
     1561 ggcacctact ttaattaaga gtcataatta ttataaatta tatatgtctt tttgactatc
     1621 tttttatatc ttctcaaatg tccgcaaagc cttattttat cggctctacg ggcattttgc
     1681 ttttgtggta aacctcgcat atctaggtct atcttcttat attttccctg tcaatcgtgg
     1741 ttaaaatcgt ggtaaatact tctatgcgat tagacgctga acctctgatt ttgcggtatc
     1801 tatggacgca tgagcgtacc agttcattgt gatactgatg tttgaatgcc ccatgatata
     1861 ctgtaaatct ttcgggttca tgttcttgct tgccagcctt gtgcagaatg tatggcgtag
     1921 cgtatgcggt gtaatatgtg gcaagggatt gtccttgtgg tgcttgttgt atttctttac
     1981 catacggata aataaggcgt tataatcaat ggcaactttt ggattacctt tcggattgat
     2041 aaacacaaaa ccgctgtgtc cgtctacctc tatcgacttt atctttggtc ggctgttgat
     2101 aacccgttga aatgcctgta tcgtttcttt gcttaatggc acttgcctta tcccgctttt
     2161 cgtcttaggc gtttcaatat aatagccctg ttccttactc tttagtaact ggtggtcgat
     2221 aatcacaact tcattcttga aatcaatgtc ggctactgtc agtccgcaca gttccgagat
     2281 acgaagtcct gtctttaaca gtatcagcac atcatcataa tgcttgtgat acacgttgtc
     2341 cgtcttaata aatgacaata aggcttgctc ctgttcctct gtcaatgcga ctttctcttt
     2401 ggtatcattt tctatgactt cacttagctt gaaatcaaaa gggtttttcc tcacataatc
     2461 atcttgtatg gctatgtaga atgacgcttt taacgagcgt ttatggttgt taatggtatt
     2521 gtaggaaaag cctttgtctt tcatgcgtaa cgcccattct ttagcgtcag agggttttat
     2581 cgtatcaatg ctcctagcac ctaacttgtc ctcttttaat aaccgcatga gttgttcccg
     2641 ttgtttctgt gtgcttttct tcacatttgt cctctgtgcg ttctgtttgg catagagttg
     2701 gcaaagcgtc attttcttgc ctgtgctgtc gataccgtcc tcaatatccc gtcttatctg
     2761 ctgttccagt tctcgaagtg agggtttttc ccgttttccc tttggtgtcg ggtctgtggg
     2821 tgtcaatctc caagcataca catattttgt gtttcccaat gcgtctacat atttatataa
     2881 gtattttccg tctgttcgtt ggctctctcc agtatgcagg atacgacctt tgttatcccg
     2941 tcttttttct ttcatggtgt ctgctccttt ccttgttgga aagagccttg atatgacttg
     3001 tgttcatcat aacacataca aggctcattt gcattagatt gcgtccaatt tatcaataac
     3061 cttttcaaac tgtcggcgtt taatctgtat gcggttgcca ttcataatga gccagccagc
     3121 gtccttgttt tcctctgcca atcgtcttaa cttgttttct ccaatgcgga aatactttga
     3181 cgcttcttca atggtaaggg tgtatttttc ccatacggga atatcctgtg tattactcat
     3241 aaaacacccc ctcactttcg attacgtctg atatgtccgt acacatctgt tccacaagca
     3301 tttcctgttt ttcagaaagg tgggtacatt catcaatcat tttcatgtag tttgaacctg
     3361 tcagcctgtc gatttcctct atcatcacga ttgacggcat gacctgtctt tccagccatt
     3421 gcagagtgcg ttccatattg acaggtacag ggttcatttc aaaaggtaaa ctgtcatggg
     3481 aaatgaatat ctcccacaat ggatagtcag gaaattccac aaaatcggta atgagataaa
     3541 acaccagccc atgagggtta tacgtcaata acagttcttc aactgcctgt accgcctttt
     3601 tgtcacgcag acgaatttca aaacggttga taatatcggt atgtttcttt ttcgttgcct
     3661 gttccttttg tttttcatac aggcagaaat attttgtgct tgcctttgag ccgatataaa
     3721 gggtacttgc taaattacgg ttttttccgc ttaattttcc accttgtaca ttttcatagt
     3781 ttttacagcg acattccgca ccaccatttc tgtatttttc agatagcaca ggaatatcca
     3841 gcagaccgca catatcattg attgccaagt caaaacgtcg gataacaccg ccacagtcaa
     3901 gacagcggtt taagaatgaa taccagtccc tgttctgtgc ctgtaagaca tattccatgt
     3961 tgcgtgagcc tgcccctttt aattctaaaa atacgcccat gtgttcatca tcagaagcca
     4021 tgaccttgat ttccccgtaa gcataatgct ccttatagcc ataataccca tagtcataat
     4081 ggataaaata atctgctttc atgccaagca cctttcggat aacttccaat gcgtcagtcg
     4141 ttggaaaacg gacagacaga taatcaacga gcagataaaa aggcttatcg cagatatagt
     4201 aattcagaca acgcaggata atgttctgtg tttccttgct ggcgttagct tttccgttct
     4261 caaagcggtt gatactatat cttgatacat gggtaagact tgcaagttct ccttgtgaaa
     4321 gaccgctggc aagacgtttg ttcttcattt caagtgcaaa ttctaaattg ttcattgtat
     4381 tcctccaatc ccacaatagc cttgaaatac ctgtaaacca cctttcaaaa agtgtgatat
     4441 gtactatctt cttaaaacct ctcattacta ctcacaggtt tgggggtgta tggtttttga
     4501 cttgtctttt gtgaaatagt gcccccctgt tagataacgg gggcacagac aaggctcgca
     4561 ggctcgccag ccgacaaagc atttcttccc cttgacacaa ggtgtcatgg cggtaagcct
     4621 gagaggcttc cgccaccgtg tcagaaatac tttgtcagca agttctgttt tgccttttct
     4681 aacaagttcc atttcaaccg atattcatat ctacggattt aatcagatag gtatttcttt
     4741 ttgaatgttt ttatatttca ttctattgtg cttattcgat tacaagaaca tagtaattct
     4801 tatatgctta tttgtcaata tctttttttg cctttacaaa tcatattaag catgatagac
     4861 ttataatagc gaagatataa cgagatatgg agggttatgc aatgaaaata agccatttag
     4921 gaaataatat acagaccata agaaaattta ggggaatgaa acaacaggaa cttgcggaca
     4981 aaatcggtat caatatgcag agcctttcca agattgaaag agggttgaac tatcctgcct
     5041 atgaaacgct ggaaaagata atggaagtgc tggacgttac gcccaatgaa ttgttgtcgg
     5101 gagaatggaa gtatgtcaat caatctgaaa aggaagtctg tcagttttta agagccgaag
     5161 aacgtctaaa tgcagaactg aaacatggac actatgataa cttctttgac agcgaggaag
     5221 aatggctgga atatgagtta gaaaagttac gggaatatat tacagactat atcaacggga
     5281 aaagcattga agcgtccgac ctttacccaa tcaaagaatt tatccagcat ttgaagtttc
     5341 aaaaattatt agaccgctat gatgatttat acagtatgga tatgtttggg gaaagcatag
     5401 agggacataa gtataagaca ccttatcaag tcgtgaaaat gataaacccg aactcaaaag
     5461 aagatatgga actgttacga gaagtattaa ggaataacaa ttttgatgat gaggacgagt
     5521 aacttttttc ctcttgcgag aagatacagc aaacaaaaag cccgatcaag agtgcaaagc
     5581 accaccaatg gtggcaaagc tcttgacagg gctttttctt ttctgttgtg ggcaatcaag
     5641 aggaagaaag ggataaaatc tgatttgtgg taaatcaggc aaaggcaacg caggtatcac
     5701 agtatgaatt gacaccgcat aaggaaagca actggaagat tgtagaataa atgaaaaagg
     5761 cttgcattct gcatttttat aaaattgcaa gtctttatat tgacaagcat aaaatactaa
     5821 gatatacttt aatcagacca acggtttgta ttggaggtaa gttatggcac gaaataaata
     5881 tccagaagaa acagtaaaac tcattttaga tgtttctaca aaaatgtttt gtgaaaaagg
     5941 atatgataac acatcattgc aagatattat caatgaaaca aaactttcta aaggtgctat
     6001 ttatcatcat ttcgattcaa aggaagatat tttaaaagca atttttcaaa gaattggaaa
     6061 tgaaaatgca gtaatatttg ctaaaattag agataacgat agtttaaacg gtttagaaaa
     6121 gttaagagaa atatttaaga cagcggtatt caattctaac caaagcgttt tattgacagt
     6181 ttctcctcaa ctattaaaca atcctcgttt tcttgctatg caaatagaac aaatgtatga
     6241 aattgtagta cctgaatttg ttgaacccgt tttagaacaa ggtatcaagg ataagtcttt
     6301 ggagattgaa aattcacatg aaattgcaga agttatcatg gtattaagta atgtatggct
     6361 caacccatta gttgaaatga cggataaaga gggtatggaa aaacgttgca atacattcaa
     6421 tgccctttta aatggaatag gcatagacac attattagac gaagaaacaa tttctgcatt
     6481 tatacagttt tgtaaataac tgtatttgtt atccctatac gatagactgc tctataaaag
     6541 agcagtttat ttaaaccatt atataaaccg acggtctgtt tgattaaata aaaaggagag
     6601 attttatgca agcagaaaaa aaacttttta atcgtgattt tatacttgtt gttataggac
     6661 agataatttc tttatttgga aacgctatcc tacgatttgc attaccgtac tatttgttaa
     6721 aagaaactgg ttcatcagct ttgtttggta ttgctacagc ctgctctttt ttacctatgg
     6781 ttattttgtc gctggtaggt ggtattattg ctgacaggac aaataaaaga aacattatgg
     6841 tcatactaga ttttgtaaca gcaatcataa ttttagtatt ttatttgtca ttgggaaaaa
     6901 ttcctatcat acctttgttt attgttgctt tgatgttact ttatggtatt tctggtgcat
     6961 atcaaccagc ggtacaagca agtattcctc tattggtatc atcagataaa cttaccgtcg
     7021 gtaatgctat aataaatcaa gtaaatactt tagcaaactt tatcggacca gctataagcg
     7081 gagtagtttt tcaattttat ggtatcaatc ccattttaat tgttagtatt atatgtttta
     7141 ctttttcagc cattatggaa atttttatcc atattccaca ccaaaaaaga cctcgtgaag
     7201 taggtgtaat tgcagttgct aaaaacgatt taaaagatag ttataccttt ttgaaaaagg
     7261 agaaacccat ttttatatcc gtaatctttt tggtttgcat tttcaatttg gttttaagtg
     7321 ctgttttaat tgttggtctg cctatcttaa tttctgaagt tttaaaaatg ccagatacac
     7381 tatatggttt ttcacaatca gcattatcat taggtggttt atgtggtggt attttaacag
     7441 ctcttgttgc ttcaaagtta aaactaaaaa acctttatac cttgttactt atttgttctg
     7501 tttctgtttt acttatggga atttcgctat ttttagaatt gcctgcaata gtttcctact
     7561 gggtagttac agtgatgtgc tttgtggcta tgggtgtatc tactatgttt gttgttcaaa
     7621 tttacactct tgttcagaca caaacaccac cgcaattagt aggaaagata atggctacgc
     7681 tggtagcagt tgctatgtgc ggtcaaccta ttggacaaat ggtttacggt attttattcg
     7741 atgtatttat acaagaaaca tgggttgtta tgtttgtagc ttctattgct tcaatcatga
     7801 tttcaatata ttcaaaaaga atttttcaaa agttagagat ataatacaag gggttaaata
     7861 gtatgttagg ctaaaaacaa tatataaaag aaaatttgaa atctgctaaa aggatagtgt
     7921 tataaaagaa agagtaatat tgcagtattt aaggctcgca caaatatatg ttttgtgtgg
     7981 gcttttttct taccattttt ctgtatttct aaagggtata aacggtattc cgtttatata
     8041 tttcagcatt agcagaaagg gggtgagatt atgaggacat cttctttcga gcatatcgtc
     8101 agattacagt tcaattcttt aatgctgatt gtcattaaga acaaagtgaa aagtcgaaac
     8161 agacagcttg caagacattc taaacgtgaa gtattgtttt gtgaaatagc cgaaacgaaa
     8221 caagttgaac gtggaacgat tgatacctgt ttttatgatt gtgtatcatt taaagtcttg
     8281 cactttacaa tttatgtatc tgatgaaaca cttggtacag ttttacatag actgtcagaa
     8341 aaacagcgta gtgcgatttt attacgttat tttcaaggaa tgaacgacag agaaatttca
     8401 gaactgtacc atgtatcacg ttctgctatt tgcagtagaa gaactagagg actaaggaag
     8461 ttaaaaatgc ttttgagcga aaggaagtaa agaaatgagt aaagattatg gctatccctc
     8521 ttttgaattg atttgcaaag cgtcaagcgg agatgaaatg gcagttaaag agattttgaa
     8581 gttttatgac ggttatattt ctaaattatg cttgcgtcct ttttaccact ctgaaagtgg
     8641 taaaatcatt atgcaggttg atgaagaact aaaaggagaa attcatacag acatgatgaa
     8701 agcaatattg aaatttgaaa tcggagtgaa gtaatcaact gtataaaaaa atcgggagat
     8761 acacagtttc gtgctatctc ccttagtagt tttacggtca tatcaaggct cattcaatcg
     8821 tggtaaattc gtggtaagat gagtg
//
LOCUS       DAYYFD010000281         5851 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.guided.132, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000281 DAYYFD010000000
VERSION     DAYYFD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.guided.132"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..1014
                     /locus_tag="ACHA25_003075"
     CDS             322..1014
                     /locus_tag="ACHA25_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8764271.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="ACHA25_003076"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="ACHA25_003076"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8764272.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="ACHA25_003077"
     CDS             complement(1910..3862)
                     /locus_tag="ACHA25_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8764273.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="ACHA25_003078"
     CDS             complement(3869..4105)
                     /locus_tag="ACHA25_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764274.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="ACHA25_003079"
     CDS             4507..4797
                     /locus_tag="ACHA25_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8764275.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(5069..5418)
                     /locus_tag="ACHA25_003080"
                     /pseudo
     CDS             complement(5069..5418)
                     /locus_tag="ACHA25_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5511..5813
                     /locus_tag="ACHA25_003081"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="ACHA25_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1997 a          925 c         1011 g         1918 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagctggaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcca t
//
LOCUS       DAYYFD010000282         4418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.guided.151, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000282 DAYYFD010000000
VERSION     DAYYFD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.guided.151"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            368..1846
                     /gene="msr(C)"
                     /locus_tag="ACHA25_003082"
     CDS             368..1846
                     /gene="msr(C)"
                     /locus_tag="ACHA25_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8764276.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1893..2339
                     /locus_tag="ACHA25_003083"
     CDS             1893..2339
                     /locus_tag="ACHA25_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8764277.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2538..3053)
                     /locus_tag="ACHA25_003084"
     CDS             complement(2538..3053)
                     /locus_tag="ACHA25_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8764278.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3170..4372
                     /locus_tag="ACHA25_003085"
     CDS             3170..4372
                     /locus_tag="ACHA25_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8764279.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1328 a          738 c          955 g         1397 t
ORIGIN      
        1 ctatgttcga ataaaaggtt ttcagttact attcagtagc tattgctttt tttgaatcgt
       61 gtgtggtaac ctaataaaaa aggaggtcgg aaaaatgact gcatcgatga aattacgttt
      121 cgaacttttg aataacaact aaataaattc aaaagacatg tataaacaac aatttcatgg
      181 atggagtatg tccatttttt cgaacgacag tagaataggt gaaagctatg acagatttgg
      241 tgatttagtc atagttattt cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa
      301 acagattttt ctgctctttt ttagtatcga taattgttgt tttacatgaa ataggagtgt
      361 aaaacatatg gaaaatttag cagtaaatat aacaaatcta caagtcagct ttggcaacca
      421 gctagaacta tctattgatt ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg
      481 agaaaatggt gtcggtaaat cgactttgct caaactaata gccggtgaac tttttcccga
      541 tcatgggaaa atccaaacag agatcacctt caactacctg cctcaattaa cctatctcgc
      601 tgaagcaaag gacctaaatt tggaattagc cagtcatttc cagttaagac tggaagaaac
      661 ttcagagcgg aaatggagcg gaggggaaga acgaaagatc gagttgatac gtcttctttc
      721 ttcttatgaa caagggatgc ttctagacga gccaacaacc catctagata gaaaaagtat
      781 tgatcgactg attgaagagc ttcgttatta ttatggtacg ctggtttttg ttagtcatga
      841 tcgctatttt ctagatgagc tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg
      901 agagttttcg gggaattata gtgcctatct cactcaaaag gaattggaga aaaagactca
      961 gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga
     1021 aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat
     1081 cagactggat cggttgtctt cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa
     1141 gaatgcgaaa acattagaga gaagactcca aaaaatagga gaaacaacca agccgcagca
     1201 gatgaaacaa atccgttttc cagtaccaaa atctcttgaa ctccacagcc gctatcccat
     1261 catgggacaa aatgtccaat tggaaagaag tgggagaaca ttactggtaa atggtgattt
     1321 tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac
     1381 cttattagaa catatccgca aacaaggaga aggaatcctt ctctctccga aagtaagctt
     1441 tcaagtatat cagcaaaagg gttatcaaat gacatctgaa gaatccatca ttcgttttgt
     1501 catgagacaa acagagtttt cagaatcgct tgtccgtagt ttgctgaatc acttagggtt
     1561 tgctcaggaa actttgacga aaccgttatg tacattaagt gggggagaag cgacccgtct
     1621 gacgattgct ttgcttttta ctaagccaag taatgtgttg ctgttagatg aaccgactaa
     1681 ttttattgat atggcaacga tcgaagcttt agagaagcta atgcaaatat atccgggaac
     1741 gattttgttt acttctcatg attcttactt tgtcgagcgt acggctgatg aagtttatga
     1801 aataaaaggg cagaaaataa aaaaagtact tacgagaaat ttttaaatgt gctacgatga
     1861 ttttcgtgac aaatttaaag gagtggtaca gattgcctac aaataaaaaa gaaggaataa
     1921 tttttacaac aatgatgtgt tttttgatgg tcttggggat gagcgcgtat aatttatggc
     1981 tgcatcaaga tcttcattta tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg
     2041 ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa aaaaattgcc tttaaactac
     2101 cagtaaatcc agaaagcaag gtccaaatga tcttaactat ttcatgcttg atggttttag
     2161 gaatggtgac atgtatgtct ttattcggtg tgatcatgga acaaggaata cctgatcaat
     2221 tattctctgc ttatatgaat gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt
     2281 tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat
     2341 ggaacactgt tatttgaaaa aaggccttgg catgcttttt gtcatagtct cttgaatgca
     2401 gttctagaaa aatagtgatc cgaacgatag gttttggcct gtcgttcgga tcgctttttc
     2461 ttttccaact gtattgctct tttattgaag ataatttgct cttcaataga tatttctaga
     2521 aaggttctgt ttatccctta ctcaaaaaac tgctgcattt gttctcttac ttgctgacac
     2581 cattggagtt cctgctgaca aagcataatt ctgcgctgga tcacaacata acgtccaaat
     2641 tcttgtttct tgtctagcga gaggtcattc aatttttttt gatttttttg catgattttg
     2701 ttcagctgtt gttctctagt gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa
     2761 aaagcataga tttttgctag aaattcatct ttttgcgtag gagcgggagt ttcctctttg
     2821 atccattcct ctacaagagc acgtccttca tcagtcaagt ggtagatctt tttttgagag
     2881 tgctcgtctt tgtccaaaac ggtcacataa ccctgctggt ctaacttggc aagcgttgta
     2941 tagatctgac tgtgatgggc ttcccaaaaa attgtgatca gctgcttcaa ttcataccca
     3001 gaacgtggtt tggataaaag cacgcataat aatatatagg ctaaagtatt cataaaaatc
     3061 ctacctctcg tcttattttc tttattatat cacagtaaag tttccacgtt ttacatgtgc
     3121 atattgacat gtaaaataaa tcatgtatga tgctaataga gagaaaggga tggaaatatt
     3181 ggagaatcaa atagatatac aagaacaaac aaatgaagaa acaaagggga aaagttcagc
     3241 gctagttgta gcagctacct gtatgatcgc ttttatggga gtgggtctag tagacccaat
     3301 cttaaaaacg attgctgcac agttaaacgc tacgccagct gaaacgaccc tgttatttac
     3361 tagttacatg ttagtcactg gtgtggtgat gctatttaca ggtttcttat ctagtcggat
     3421 cggtttgaaa aaaacattga tgacaggatt gtttatcatc gttgtattcg ctggtcttgg
     3481 cggattatct gggaatatcg gaatgctgat tgggttacgt gccggctggg gtgtcgggaa
     3541 tgctttattt gtctctactg ctttagcaac gatcgtcagt attttagttg gtaacacaga
     3601 aaaagcaatc atgatgtatg aagcagcact tggctgcgga atggctgttg gccctctagt
     3661 cggtggggtc ttgggcagtg gttcatggcg ttatccattt tttggggtag catttttgat
     3721 gttttgcggt tttatagccc tagcggtact tttacctgaa actgagaaac caaagagaaa
     3781 agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt cagttggact
     3841 aatcgcattg ttatataatt tcggattctt tactttactt gcgtatgcgc catttttatt
     3901 gattggctat tctgaattag aagtcggttt tgtatttttt ggctggggtg tgcttctggc
     3961 tattgcttcg attttcgttg caccggctct tgagcggaag tttacgactt atcggacaat
     4021 tttagcagca tttgtcttat tcattatttg tttgctgtta ttaggagctg gaacttctat
     4081 gccagtcctt gtaccgatca gcattgtgtt agcaggattt ttccaaggaa ttatcaatac
     4141 actgttgacg acgattgcta tggaaatacc aggtctgcaa cgaaacgttg cttcgtccag
     4201 ctatagtttt gtccgctttt tcggtggagc tttagcacca tttattgcag gtgtgattgg
     4261 tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt gccattgttc ttatcagtgt
     4321 atgttgtatc atttatcacc gtaattattt tgctacaaat gaagaagaat aagaaatcag
     4381 aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DAYYFD010000283         3795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.guided.162, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000283 DAYYFD010000000
VERSION     DAYYFD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.guided.162"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHA25_003086"
     CDS             110..193
                     /locus_tag="ACHA25_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8764280.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHA25_003087"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHA25_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8764281.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHA25_003088"
     CDS             complement(1318..2181)
                     /locus_tag="ACHA25_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8764282.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHA25_003089"
     CDS             complement(2183..2455)
                     /locus_tag="ACHA25_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8764283.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHA25_003090"
     CDS             complement(2472..2681)
                     /locus_tag="ACHA25_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8764284.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACHA25_003091"
     CDS             complement(2759..3655)
                     /locus_tag="ACHA25_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8764285.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1373 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccct
//
LOCUS       DAYYFD010000284          923 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6631
            SAMEA115708493-rid22596413.guided.225, whole genome shotgun
            sequence.
ACCESSION   DAYYFD010000284 DAYYFD010000000
VERSION     DAYYFD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708493
            Sequence Read Archive: ERR13198215
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290289.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290289.1
            Genome Coverage       :: 127x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,092
            CDSs (total)                      :: 3,031
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 33 of 86
            Pseudo Genes (incomplete)         :: 37 of 86
            Pseudo Genes (internal stop)      :: 31 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708493-rid22596413.guided.225"
                     /isolate="VRE6631"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            310..858
                     /gene="aac(6')"
                     /locus_tag="ACHA25_003092"
     CDS             310..858
                     /gene="aac(6')"
                     /locus_tag="ACHA25_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8764286.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          334 a          131 c          187 g          271 t
ORIGIN      
        1 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
       61 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
      121 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
      181 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
      241 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
      301 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
      361 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
      421 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
      481 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
      541 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
      601 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
      661 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
      721 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
      781 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
      841 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
      901 gaaggaattt acgaaaatta ctt
//
